LOCUS       DASRMS010000001         1981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_872443, whole genome shotgun sequence.
ACCESSION   DASRMS010000001 DASRMS010000000
VERSION     DASRMS010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1981
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872443"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1039
                     /locus_tag="VFC74_00005"
     CDS             <1..1039
                     /locus_tag="VFC74_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23757.1"
                     /translation="IQAKWLLEGTLPRAALPRWNACQRYLIDRLKPAGADVGAKDASR
                     VLRLVETVNSKSNEICRVVHVENGADGQPVRYNFEYLAEVLLPVARWDIEKQKQERDE
                     RQKRKQLKLLDGYKVSSNLRGFSGRQLAWHRLEDLRTLATLRGGVAEGDRMRHLHWQL
                     NFLLLSGATNSSLMYHEAAALAREINPGWAYNSKELMSLYSKARQFEAGEKINFGGKD
                     YPPLYTPRNDTLINLFEITDDEQAQLRTIISKDVAACRHKDRETVRRRAAGAVDRETY
                     LNAANEREKQAHELRGLGYTVRGIAKQMAVSVGAVSGYLKTYVSAVDKETVIAAKNVC
                     SKSVRITNGVA"
     gene            1712..>1981
                     /locus_tag="VFC74_00010"
     CDS             1712..>1981
                     /locus_tag="VFC74_00010"
                     /inference="COORDINATES: protein motif:HMM:NF023168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZK23758.1"
                     /translation="MTKSNIPVEVRDRIIAAAEEIYEQLGRETFPTVDTVRRAAKADM
                     NTTSMVMKDWRRQQTTQAVPVAVTVPEAVVQANTSALAQLWAEAQE"
BASE COUNT          535 a          383 c          510 g          553 t
ORIGIN      
        1 catacaggct aagtggttgc tagagggcac gttgccccgt gctgcattgc ctcgctggaa
       61 cgcttgccag cgttatctaa ttgaccgatt gaagccagca ggtgcagacg taggcgctaa
      121 agatgctagt cgggtacttc gtctggtcga aacagtaaac agtaaaagca atgaaatctg
      181 ccgcgttgtt cacgttgaaa acggagcaga cggccagcct gtacgctata actttgagta
      241 tttagcagaa gtgcttttgc cggttgccag atgggatatt gaaaagcaaa aacaagagcg
      301 tgacgaacgc cagaagcgta agcagcttaa attgctggat ggttacaagg tcagcagcaa
      361 cctacggggc tttagtggcc gccaactcgc atggcatagg ctagaagacc tgcgcacctt
      421 agccacattg cgcggtggtg tggccgaggg cgacagaatg cgccacttgc actggcagct
      481 aaattttttg ctgctatcag gtgcaacaaa cagcagcttg atgtatcacg aagctgcagc
      541 attagctcgt gagatcaatc ctggttgggc atataacagt aaagagctga tgtcgctgta
      601 ctcaaaagca agacagtttg aggcaggtga aaaaatcaat tttggtggaa aagattatcc
      661 accactttac actccgcgca acgacacatt aatcaatctg tttgaaatca ctgatgacga
      721 gcaggctcaa ttacgcacga tcattagtaa agatgttgct gcatgtcgtc acaaagaccg
      781 cgagacagtc cgtaggcgtg cagcaggagc tgtagataga gaaacatatc taaatgcagc
      841 taatgaacga gagaagcagg cccacgaact gagaggcctt ggttacactg tcagaggtat
      901 tgccaagcag atggctgtgt ccgttggtgc cgtttcgggt tatttaaaaa cctacgtatc
      961 tgcagtagat aaagagacag taattgctgc aaagaatgtg tgttcaaagt ccgtgcgtat
     1021 tactaatggc gtagcctagg tgctctagtg acttagctct taggcttctg aaaagatatt
     1081 tatatctgtt gttaggagcg aaagtgagtg ctcgttagtg ttggtcaagt caaacacata
     1141 taactggtta acaggctgtg tgtttggctt gtaatggtta aaaaagggcc ggctagggtt
     1201 tgtatgggtt gccttgtttc gttgcgtgta cggctttttg gagcgcttta gtggtcagct
     1261 aaacgtattc accagcgtaa agtgcacaat tcaggctgaa aatattaagt gttcaaagtc
     1321 catgcgtatt actaatggcg aagcctaggt gctttattga tttggctttt aggcttctaa
     1381 aaagatattt atatctgttg ttaggaacga aagtgagtgc tcgttctagt tggcggctgg
     1441 ttcaacacgc ataacttatt aactggctgt gttgttttct ttccttagct gttttgcagg
     1501 ctaaagcatg cgctggctag ggttcgcgtt ttggtggtga gtgtacggcc ttccagggcc
     1561 atctaaagtt ctcgcgtatt actaatttga tctttagggt atttggtctc tttgcttgct
     1621 ggcaaaagat aattctattt catattgcta aataataaga ataataatat aattcttatt
     1681 attcttatta ttcttattca aggtgcctga catgactaaa agtaatattc ctgtagaagt
     1741 tagagatcgt attattgctg ctgctgaaga gatttatgag caattagggc gcgaaacatt
     1801 ccctacagta gatactgttc gccgtgctgc taaagctgat atgaatacga caagtatggt
     1861 tatgaaggat tggcgcagac agcaaacgac gcaggctgtc cctgttgcgg taactgttcc
     1921 tgaagctgta gtgcaagcta atacttctgc gcttgctcag ctgtgggcag aagctcaaga
     1981 g
//
LOCUS       DASRMS010000002        11254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_537138, whole genome shotgun sequence.
ACCESSION   DASRMS010000002 DASRMS010000000
VERSION     DASRMS010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 11254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11254
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537138"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..795
                     /locus_tag="VFC74_00015"
     CDS             <1..795
                     /locus_tag="VFC74_00015"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZK23759.1"
                     /translation="TTVLLIVIALTALAFSLNVGARVYRLFYGVKAGVVLEEIPMERL
                     LEKEVREKVRQMAGAIDQAPQNARFEQSTMEVTPEVVGIKVDIETTVAAVMQAAAEEK
                     VALVVIRLEPEVTAAVFRSINTKIASYHTSGGGSAGRTDNLYMAAKYMHGTVLAPGDI
                     FSFNHVTGPRTFERGYSMAPVVGGLGIGGGVCQVATTVYNVALIAEMEIVERHPHSIR
                     VGYVPPGKDATVTNYIDFKFRNSTDKFVQIRSGAGGGSVWVELWSQ"
     gene            864..1307
                     /gene="dut"
                     /locus_tag="VFC74_00020"
     CDS             864..1307
                     /gene="dut"
                     /locus_tag="VFC74_00020"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020074532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZK23760.1"
                     /translation="MCKLFVQKLPHAADLPLPAYMTTEAAGMDLLAALTEPLPLLPGS
                     RVLVSTGLQMALPSGFEGQIRPRSGLALKHGITVLNSPGTIDADYRGEIKIILINLGQ
                     EPYTISPGERIAQFVLSRVENATLVLCEELPASVRGGGGFGHTGR"
     gene            1319..1486
                     /locus_tag="VFC74_00025"
     CDS             1319..1486
                     /locus_tag="VFC74_00025"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HZK23761.1"
                     /translation="MPIADEAQLQVLQAALATAIWKNEGIISCPEVISISQELDQLII
                     KLMRAERDELV"
     gene            1483..1803
                     /locus_tag="VFC74_00030"
     CDS             1483..1803
                     /locus_tag="VFC74_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23762.1"
                     /translation="MTLLFVLIVLLSCSLYLRMADQVRTYASQLPEHAKSSPFSVALQ
                     ELLAQSGGIYLSLVLLVSFLNIEIADKWRIMGMDTEPIALLALVVAALQPLFLKIYRM
                     FKGR"
     gene            1809..2078
                     /locus_tag="VFC74_00035"
     CDS             1809..2078
                     /locus_tag="VFC74_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HZK23763.1"
                     /translation="MELSQLSRKEIINLHDGSRLGFVGDSDLVIDDQSGYIQAIIIYA
                     KGGAGKVTNARELVIPWEAVKIIGEEVLVVDIAPDHGSRKYSRYE"
     gene            2140..2625
                     /locus_tag="VFC74_00040"
     CDS             2140..2625
                     /locus_tag="VFC74_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HZK23764.1"
                     /translation="MSTVVGLFRSEDQAEEAIRELRAREFDDLDVSLVARGEGDDREA
                     DNASLNFDRQNLTDGTATGGAIGGLAGLLAGAGALLIPGLGPIIAVGPLAGALTGAVT
                     GGIAGGLIDYGIPETEGERYEQEIQKGSVMVAVEADEDLEAEVAAVFRENGAFDVKTH
                     D"
     gene            2728..3612
                     /gene="dpsA"
                     /locus_tag="VFC74_00045"
     CDS             2728..3612
                     /gene="dpsA"
                     /locus_tag="VFC74_00045"
                     /inference="COORDINATES: protein motif:HMM:NF006162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit DpsA"
                     /protein_id="HZK23765.1"
                     /translation="MQQPTLKGYDIAIIGGDRREVFLAEELLNRGANVWLYGFLQFKL
                     PIEQRIKQTLPKKVDIIILPLPGVLADHSIYSAYTTERIYFAELNHLLQSGVLLFCGK
                     MPDFLRQQLQEQGLHVILASDLAELTIFNAVPTAEGVLEIAMRESQITFNNSSVLITG
                     FGSCAQQLARISSALGAKVTVVARKQKDITAAKVLGYDALNFTELTALMSNFNFIFNT
                     VPNLVLNKEVLLHVRKSVLLYDIASAPGGTDFAAAQELGLQARLLPGLPGKVAPLTVG
                     KQLAEVYDYYINLHRKED"
     gene            3617..4228
                     /locus_tag="VFC74_00050"
     CDS             3617..4228
                     /locus_tag="VFC74_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipicolinate synthase subunit B"
                     /protein_id="HZK23766.1"
                     /translation="MRLKDKKIGFGITGSHCTLEPILPEIDRLIKEGAEVYPVISPAI
                     DFTDTRFGDAIEWKTKLVKATGRKLINSIVGVEPLGPSKLLAAYVIAPCTGNTLAKIA
                     NGITDTAVLMGAKAHLRNQRPLIVAISTNDGLGLNAKNISTLLIAKNVYLVPFGQDQP
                     FTKPNSLIAKMELLVPTILAALEGRQLQPLLINHAQEEFQLKS"
     gene            4240..5466
                     /gene="dapG"
                     /locus_tag="VFC74_00055"
     CDS             4240..5466
                     /gene="dapG"
                     /locus_tag="VFC74_00055"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZK23767.1"
                     /translation="MRLLVQKFGGSSLATQELRQQVVEHIVTAKQAGWQVVVVVSAIG
                     RQGDAYATDTLLDFLYQLTTTPPPREKDLLLACGEIIAAAVTAAAVNEAGEEAVALTG
                     WQAGIITDGAFGQAEIVKIKKERILRHLQDNQTVIVAGFQGISDDAEITTLGRGGSDT
                     SAVALGAALNAELVEIYSDVDTVMTADPRLVPEAQPIKNIGYQEVLQMAHQGAKVIHP
                     RAVDLALQYNLPLLLKKTGASAPGTLITHHKLGNGQRLRQSSNVVTGITHTNNLAQVC
                     VQAPSTDGYQLEELLKKLSSAGISIDLINFSPQEKMFTVSASHQEKVKQILQELGYKA
                     QVAPGFAKVTVVGSGMRGIPGVMARIVSALNKAQTPILQTADSHLNISCLVAEDNVTA
                     AARALHLEFGLHEDSI"
     gene            5548..6435
                     /gene="dapA"
                     /locus_tag="VFC74_00060"
     CDS             5548..6435
                     /gene="dapA"
                     /locus_tag="VFC74_00060"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HZK23768.1"
                     /translation="MIAPFGEVLTAMITPFTDDGEVNYEEAVRLATYLADNGSDAIVV
                     AGTTGESPTLTDEEKIKLFTTVAEAVKGKAKVLAGTGANSTKHSVELTKKAETSGVDG
                     IMLVVPYYNKPTQEGLYQHFAAIAKATTLPVLLYNVPGRTSLNMAAETTVRLAAAVTN
                     IVAIKEASGNLEQIARIRSTAPPDFAIYSGDDALTLPILAVGGCGIVSVASHVAGKEI
                     KKMVSAYRAGDTATALTIHLRLLPLFKAMFMVTNPIPVKTAMHMLGFKTDVLRLPLVA
                     PTLVEKAPLEEVLTRMGYL"
     gene            6670..8379
                     /locus_tag="VFC74_00065"
     CDS             6670..8379
                     /locus_tag="VFC74_00065"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HZK23769.1"
                     /translation="MSNGQSRRRAKVKHVANPTRKVQVIPIGGIGEIGKNMYVIRYED
                     EIIIIDAGMSFPTEEMFGIDVVIPDITYLLENQDKIQAVLLTHGHEDHIGGLPYFLQK
                     INVPVYGTKLTLGLVEVKLREHRMLADCTLHTIQPEDKLELGSFKIEFFRTNHSIPDS
                     VGMAVKTPVGTIVHTGDFKFDQTPVSGQVTDYYRLAKLGEKGVLVALSDSTNAERPGY
                     TYSEKEVGSAIMDIFRTARQRIILATFASNIHRIQQVIDAATHYKRKVAVVGRSMVNV
                     VSIAQELGYLHAEAGTIVEVDEINKMPPHKAVILTTGSQGEPMAALTRIARAEHRKVE
                     LIPGDTVIISASPIPGNEKLVSRTIDNLFALGADVIYERVSGVHVSGHASQEELKLML
                     NLLKPKYLIPIHGEYRHLIHHAQVAEKVGIPSDNVFIMENGQVLEFTPGSARIVGNVP
                     AGHVLVDGLGVGDVGSIVLRDRKLLSEDGIIMVVMTIMRENGEIMAGPEIYTRGFVYV
                     RESEILLDEAREHVYKSITKALQGRNTDWSSVKGQMRESLSRYIWEKMHRRPIILPVI
                     MEI"
     gene            8547..9266
                     /locus_tag="VFC74_00070"
     CDS             8547..9266
                     /locus_tag="VFC74_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HZK23770.1"
                     /translation="MPATAPDIPVKPEANHENALQNIEQLGQTTVPVQPEGNIHTISI
                     IGQIEGHLTLPPQNKTTKYEHIIPQLVAIEQNPKIQGLLIILNTVGGDVEAGLAIAEM
                     IDTLTKPTVSLVLGGGHSIGVPIAVSTDYSFITETATMTIHPIRLSGLVIGVPQTYEY
                     LDKMQDRVIKFVTNHSRISSEKFRRLMFCTDQLARDIGTVMVGKDAIEDGLIDEVGGL
                     GAAIAKLQSLIDGHYPSEVIH"
     gene            9266..9496
                     /locus_tag="VFC74_00075"
     CDS             9266..9496
                     /locus_tag="VFC74_00075"
                     /inference="COORDINATES: protein motif:HMM:NF025401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlzJ-like family protein"
                     /protein_id="HZK23771.1"
                     /translation="MLWTVMPLEMVMEGSDSFAPTYTEISWQESTLVVEPLEMNTARV
                     VRIISTNPEDYLNPQVQPGSIIQIRNNQPDLS"
     gene            9697..10659
                     /locus_tag="VFC74_00080"
     CDS             9697..10659
                     /locus_tag="VFC74_00080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23772.1"
                     /translation="MRGKALLILLSMLLVLTGCAAPFSEVQVAEEIAACYQLLSPTVV
                     TINLLATLPHHGNLLVLVADHSAAEAPLMDLFLLNTKGVVSLASAPLTNKIKLHSLVD
                     GNETLLYGPLPTAAEDGIPVENLPDAIDTLQTITGKAAITLNSWKIIEQQLPIAKGFI
                     LALKQKRQAEAVNLYDETGKILAQFAKLSKGAMKETSFTAWDELPLPRELEQENMAAV
                     QVAASVLAAEDEGVSLAEEEVREPLIAAFNACLPELRRKGSEAADTNLVVTIHLNDGS
                     SVLIKQHNSNFTISFERAEVTLNYEASSSELAALFKTYSKLLKL"
     gene            complement(10665..11228)
                     /locus_tag="VFC74_00085"
     CDS             complement(10665..11228)
                     /locus_tag="VFC74_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23773.1"
                     /translation="MRKKRYFQNLYKFEKESQHYLIEVSLDSYNDVYDSWDPSPFKRR
                     DIEDEFHDFIYNSSEDIPLAYQLSIVLYLPLAKQDEKKEQAVISAYHNYYDYAQARLA
                     KTMNKLNRKIVTSLLLAVLFLTVGYFWGSKVTGILFNVLQEGIFIGGWVFLWDFFTDI
                     FITKRAQQDEFKLYHRLSQADLRFVYQ"
BASE COUNT         3282 a         2223 c         2670 g         3079 t
ORIGIN      
        1 actaccgtat tattaatagt gattgcctta acagcacttg cgttttcatt aaatgtgggt
       61 gcacgcgtct accgcttatt ttatggtgtt aaagctggtg tggttttaga ggaaataccg
      121 atggagcggc tgttagagaa agaggtccgt gaaaaagttc ggcagatggc tggagctatt
      181 gatcaagcac cccagaatgc gcgctttgag caaagtacga tggaagttac accagaagtt
      241 gtagggataa aagtggatat agaaactacg gttgccgctg taatgcaggc tgccgcagag
      301 gaaaaggttg ccttggttgt gattcgttta gaaccagagg taacagcggc cgtctttcgc
      361 tccattaata caaagatcgc ctcctatcat actagtggcg gtggcagtgc gggtcgtaca
      421 gacaatttgt atatggcagc caaatatatg catggtactg tgctggcacc aggagatatc
      481 ttctctttta atcatgtgac gggaccccgt acctttgagc gtggttacag catggcgcct
      541 gtggtcgggg gattgggcat tggcggaggc gtctgtcaag tcgccactac tgtttacaat
      601 gtagcgttga ttgccgaaat ggagattgtg gaaaggcacc cgcatagcat acgcgtaggt
      661 tatgtaccac cagggaaaga cgctacagtg accaactata ttgattttaa atttcgcaat
      721 tctacggata agtttgtcca aattcgctca ggtgctggcg gtggttctgt gtgggtggaa
      781 ctatggagtc aatagtcctg caatattgac aaaacaaaac aaaggcagta agctggggaa
      841 aactatgcca aaggagtatt acgatgtgta aattatttgt gcaaaaactt ccccatgctg
      901 ctgatttgcc tttaccggct tatatgacaa cagaagcggc aggaatggat ctgctggccg
      961 ccctaaccga acctctccct ttgctgccag gaagcagagt actagtgtct acaggtctgc
     1021 aaatggcctt gcctagtggt tttgagggac aaatacgtcc acgcagtggt ttagcgctta
     1081 aacatggcat tactgttctt aatagtcccg ggaccattga tgctgactat cggggcgaaa
     1141 taaaaatcat tctcattaat ctagggcaag aaccctatac catttctcct ggcgaacgta
     1201 tagcgcaatt tgtactaagt cgggtagaaa atgctacttt agtcttatgt gaggagctgc
     1261 ctgctagtgt aagaggcggc ggtggtttcg gacatacagg tagataggag gactgccgat
     1321 gccgatcgct gatgaggctc aactgcaggt actgcaggca gccttagcga ccgctatctg
     1381 gaaaaacgaa ggtattataa gttgtcccga agttattagc attagtcagg agctagacca
     1441 actgataata aagctcatgc gtgctgaaag ggatgaactt gtttgaccct gctctttgtt
     1501 ttaatcgtgc tgctcagctg ttcactttat ctgcggatgg cagatcaagt gcgtacttat
     1561 gcctctcagc tgccggaaca tgccaagtct tctccctttt ctgtggccct acaggaactt
     1621 cttgctcagt ccggtggaat ttatttgtct ttagtcctct tggtttcatt tttaaatata
     1681 gaaattgcgg ataaatggag aattatgggt atggacacag agccaatagc tttattggct
     1741 ttagtagttg ctgcactgca acctcttttt ttaaagattt atcgtatgtt taaagggaga
     1801 tgaaattaat ggagttatca caactgtcac gtaaagaaat tattaatctt catgatggct
     1861 cacgacttgg ctttgtcggt gactctgatc ttgtgattga tgatcaatct ggttatattc
     1921 aagcaatcat tatttatgct aaaggtggtg ccggaaaagt tactaatgcc cgggagttag
     1981 taattccttg ggaagcagta aaaataatag gtgaagaagt tctagtggtt gatatcgcgc
     2041 ctgaccatgg ttcacgcaag tattcacgtt atgaataatg catctttttc ggttgatact
     2101 agaaaagaaa ttagcatcaa ctgaaagagg aggattcata tgtcaacagt agttggtttg
     2161 tttcgttctg aagaccaagc agaagaagcg attcgtgaat taagagcgcg tgaatttgat
     2221 gatctggatg tctctctggt tgcccgtggt gaaggggatg acagagaagc ggataatgcc
     2281 agcctgaact ttgatcgcca aaacttaacc gatggcacag caacaggagg tgccatcgga
     2341 ggtttagctg gccttttagc tggtgctggt gctctgttga tcccggggct gggaccgatc
     2401 atcgccgtgg gtccgctggc tggtgcctta acgggagccg taaccggtgg tattgccggt
     2461 ggtttaatag attatggtat tccggaaaca gaaggagagc ggtacgagca agaaattcaa
     2521 aaaggtagtg tgatggtagc agtagaagct gatgaagacc tcgaagctga agtagccgcc
     2581 gtattccgtg aaaatggtgc ctttgacgta aaaactcacg actaaaagaa aaatctggca
     2641 actgccagat ttttttttgc catattatca ccctttacaa gccccctctc atatattgaa
     2701 ttacatggaa cgttgagggg tgacaggatg cagcagccaa ctctaaaagg ttacgatatc
     2761 gccatcatcg gtggtgacag acgtgaggta tttttagcag aagaattatt aaatagggga
     2821 gcaaatgtgt ggttatatgg tttcttacag tttaaattac ctattgagca gcgcataaag
     2881 caaactttac ctaaaaaggt tgacattatt attttaccgc tgcctggagt tcttgctgat
     2941 cacagtatct atagtgccta taccactgaa aggatatatt ttgccgagct aaaccattta
     3001 cttcagtctg gtgtcttatt gttttgtggt aaaatgcctg attttttacg gcagcagttg
     3061 caagagcaag gtcttcacgt catacttgcg tctgatttag cagagttaac tatttttaac
     3121 gctgttccta cggcagaagg tgtactagaa atagcaatgc gtgagtcaca aatcacgttc
     3181 aacaatagct ctgtgttaat taccggcttt ggcagctgcg cacaacagct agctagaatt
     3241 tcttcagctt taggagccaa ggtcactgtt gtggcccgta aacaaaagga tataaccgca
     3301 gcaaaagttt tgggctatga tgccttaaat tttacagaac tgacagcact gatgtcaaac
     3361 tttaatttta tcttcaatac tgtaccaaat ttagtgttaa acaaagaagt attattacac
     3421 gtgagaaaaa gtgttttgct ctatgatatt gcctcggcac ccggcggtac agattttgcc
     3481 gcggctcagg aacttggttt acaggcaaga cttttaccgg gcttacctgg taaagtagcg
     3541 ccgctgacag tcggcaaaca attagctgaa gtttacgact attatatcaa cttacataga
     3601 aaggaggatt aagaccatga ggcttaagga caaaaaaatc ggttttggca ttaccggatc
     3661 acattgtaca ttagaaccca ttttgccgga aatcgataga ttgattaaag aaggagcgga
     3721 ggtctatcca gtaatttcac cagcaattga tttcacagat acgcgctttg gtgatgccat
     3781 tgaatggaag actaaactgg taaaagccac gggacgtaaa ttaatcaata gtattgtcgg
     3841 tgtagaacca ctaggaccaa gtaaactgtt ggctgcttat gtcattgcac cttgtacggg
     3901 aaatacctta gcgaaaattg ccaacggtat tactgacaca gctgtgctga tgggagcaaa
     3961 agcacatcta cgtaatcaaa gacctttgat tgtagcaatt tctacaaatg acgggcttgg
     4021 tttaaatgct aaaaatatca gcactttatt gatagcgaag aatgtctatc tggtaccatt
     4081 tgggcaagat caacctttta caaaacccaa ttcactgatt gccaagatgg agctgctggt
     4141 gccaacaatt ttagctgctt tggaagggcg gcagctacag ccgctattaa taaatcatgc
     4201 tcaagaagaa tttcaattaa aaagttagaa ggtggattta tgcgcctgct tgtgcaaaaa
     4261 ttcggtggtt cttccttagc cactcaggag ctcagacagc aggttgtaga acatattgtc
     4321 acggccaaac aagcaggatg gcaagtggtg gtggttgttt cggctattgg ccgccaaggt
     4381 gatgcctatg ctactgatac tttattagat tttctctacc agcttacaac aacaccgccg
     4441 ccgcgggaga aggatttact gctggcttgt ggggaaataa tcgcagctgc agttacagca
     4501 gctgcagtaa atgaggcggg tgaagaggca gtcgctttaa ccggctggca ggctgggatc
     4561 atcaccgatg gcgcgtttgg tcaggcggaa atagtaaaaa taaaaaaaga acgaattttg
     4621 cgacacttgc aggataatca gacggttatt gtagcaggat ttcaaggaat tagtgatgac
     4681 gccgaaatta caaccttagg acgcggcggt tctgatacct ctgctgtggc gctaggtgcg
     4741 gctctaaatg cagaattagt agaaatttat tctgatgtag atacagtgat gacggcagac
     4801 ccacgtctgg tgcctgaagc tcaaccgatt aaaaatattg gttatcagga agttttacaa
     4861 atggctcatc aaggagcaaa agttattcat ccacgtgctg ttgatttggc tttacaatat
     4921 aatttacctt tgctgttaaa gaaaacaggg gcgtccgcac cgggtacact gattacccac
     4981 cataaattag gtaatggtca aagattacgc caaagtagta atgttgtgac aggaataacc
     5041 cacactaaca atttagcaca ggtctgtgtg caagctccct ctactgatgg ttaccagtta
     5101 gaagaactgt tgaaaaaact ttcaagcgcg ggtattagta tagatttgat aaatttctca
     5161 ccacaagaaa agatgtttac agtctccgcc agccaccaag aaaaagttaa acaaattttg
     5221 caggagcttg gttacaaagc tcaagtggcc cctggatttg ctaaagtaac agtggtagga
     5281 tcaggtatgc gtgggattcc cggtgtgatg gcccgcattg tgtcagcgtt aaataaagcg
     5341 caaacaccaa ttttacagac cgcggattcg catcttaata tatcttgttt agtggcagaa
     5401 gataacgtaa cggcagcagc tcgtgctttg cacttggaat ttggtttaca tgaagattct
     5461 atttgaatat tttaacttct atgatataat gtcataacta ggtataaata aaacttttag
     5521 aggaactaaa gctggaggtg acttttgttg attgcacctt ttggtgaagt attaacagca
     5581 atgatcacac cgtttacaga tgacggtgaa gtgaattatg aggaagccgt tagattagct
     5641 acatatctgg cggataacgg cagtgatgcc atcgttgtgg ccggaactac cggggaatct
     5701 ccgaccttaa cagatgaaga aaaaatcaag ttgttcacga ctgttgcaga agccgtcaaa
     5761 ggtaaggcga aagtgcttgc cggaaccggt gctaattcca ccaagcactc tgtggaatta
     5821 actaaaaaag ctgaaacaag cggcgtggac ggcattatgt tggtagtgcc ctattacaac
     5881 aaacctactc aggagggttt gtatcagcat tttgcggcca tcgctaaagc gacaacgctc
     5941 ccggtgctgc tttataatgt gccaggtcga acgtcactaa atatggctgc ggagactacc
     6001 gttcgtttag ccgctgcggt gaccaatatt gtggcgatca aagaagccag tggtaattta
     6061 gagcagattg cccgcattcg cagtactgcg ccgccggact ttgcaattta tagcggggat
     6121 gacgctctga ccctgccgat ccttgctgtg gggggctgtg gtatcgtctc tgtggcctca
     6181 cacgttgccg gcaaggaaat taaaaaaatg gtttcagctt accgggcagg tgacacagca
     6241 acagcattaa ctatccatct tcgtttgctg cccttgttta aagcaatgtt catggtgact
     6301 aatcctattc cggttaaaac tgccatgcat atgcttggct tcaaaacaga tgtgctgcga
     6361 ctgccgctag tggcaccaac tttagtggaa aaagcaccat tagaagaagt attaaccagg
     6421 atgggttatc tatagtcagc aaaaccgtgt tcggcacggt tttttttttg ctaatttagg
     6481 aagccagcgc ctcgtaataa acaaatgact agagacgccc ttctaaatgg catgcaatct
     6541 gaagaaaacg caagctttct ttaagctgcc ttgcatattt tatgcggact tgcatccgct
     6601 tcttcaattc agttataatg gtagctagtg atatttggac ggaaacaaat tagaaggagg
     6661 tgaagtattt tgagtaatgg tcaaagtaga cgcagagcaa aggtaaaaca tgtggcaaat
     6721 cctaccagaa aagtgcaggt cattccgatt ggtggtatcg gagaaatcgg taaaaacatg
     6781 tatgtcattc gctatgagga tgaaataatc attattgatg ccggcatgtc atttccgaca
     6841 gaagaaatgt tcgggattga tgtggtcata ccagatataa catatctgct ggaaaatcaa
     6901 gataagatac aggctgtcct cttaacgcat ggtcatgagg accatattgg cggattacca
     6961 tattttttac aaaagatcaa tgtgccggtt tatgggacaa aattaacttt aggccttgtt
     7021 gaggttaaat tacgcgagca ccggatgtta gctgactgta ccttacatac tattcagcct
     7081 gaggataagc ttgaactggg ttcttttaaa attgaatttt tccgcaccaa tcacagtatt
     7141 cccgattccg ttggcatggc agttaaaaca ccagtcggca cgatagtcca tactggcgac
     7201 tttaagtttg atcaaactcc cgtcagtggt caggtcactg attattatcg cttagcaaaa
     7261 ttaggagaga agggtgtgct ggtagcactt tctgatagta ctaatgccga gcgccccggt
     7321 tatacctatt ctgaaaaaga ggttggcagt gcgattatgg atatcttccg tacagctcgc
     7381 caacgcataa ttcttgctac ttttgcttct aatatacacc gtattcagca agtgattgat
     7441 gccgctacac actacaaacg aaaagttgca gtagtcggcc gcagtatggt caatgtcgtt
     7501 tcgatcgcgc aagaacttgg ttatctgcat gccgaagcag gcacgattgt agaagtagat
     7561 gagatcaaca aaatgccgcc gcataaagca gtaatcctta ctacggggag tcagggtgag
     7621 ccgatggctg ccctaacacg gattgccagg gctgagcaca gaaaagtcga attaatacct
     7681 ggcgatactg ttatcatctc ggcgtcacca atcccgggta acgagaaact ggtgtcgcgt
     7741 actattgata atctctttgc gctaggagcc gacgtaattt atgagcgtgt ttctggtgtt
     7801 catgtctcag gtcacgcctc gcaagaagaa ttaaagttaa tgctaaatct gcttaaaccc
     7861 aaatatctaa ttcctattca tggtgaatat cgacatctta ttcaccatgc gcaagttgct
     7921 gaaaaagtag gcattcctag cgataacgtg tttatcatgg aaaatgggca ggtattagag
     7981 tttacgcctg gatctgctcg tattgtcggc aacgtgccgg caggacacgt actagtagat
     8041 ggtttgggtg ttggtgatgt cggcagcatt gtcttacgcg atagaaaact gctgtctgaa
     8101 gatggcatta tcatggtagt aatgacgatc atgagggaaa acggcgaaat tatggctggc
     8161 ccagaaatct acacacgtgg ttttgtctat gtgcgcgagt cagaaatctt gctggatgaa
     8221 gcacgtgagc atgtttataa gtccataaca aaagcacttc aaggtagaaa taccgattgg
     8281 tccagtgtaa aaggtcagat gagggaatcc ttgtcccgct atatctggga aaaaatgcat
     8341 cggcgtccaa tcatcttgcc ggtgatcatg gaaatttaaa gtctgcatgg aattctctag
     8401 ttctgggtgc gtgcgaacat tagcttcagt ataagagtca gtctgggcga aatactagta
     8461 cagagattct gatgaggatg tgaagcataa tgactagtaa tcaccgccga cagcgcacgc
     8521 cccaaactac acgcagaccc cgcactatgc ctgccacagc gccagacata cctgtcaagc
     8581 cagaagcaaa tcatgaaaat gctctgcaaa acattgaaca actaggacaa acaactgttc
     8641 ctgttcagcc tgaaggaaat atacatacta tctctatcat cgggcaaatc gaaggtcatc
     8701 tgacattgcc gccacagaat aaaacaacaa aatatgaaca cattattccc caactagtgg
     8761 cgattgagca aaaccctaag attcagggac tgctaataat ccttaataca gtaggcggag
     8821 atgtggaggc aggtttagcc attgcggaaa tgattgatac attaactaaa cccactgttt
     8881 cattagtgct cggaggtggc catagtattg gtgtgcccat tgctgtcagc acagattatt
     8941 cttttatcac cgagacagca accatgacca ttcaccctat tcgcctatct ggtctggtta
     9001 ttggcgtacc acaaacttat gagtaccttg ataaaatgca ggatcgagta ataaaatttg
     9061 ttactaatca ttctcgtatt tcgtcggaaa aatttcgtcg tttgatgttc tgtactgatc
     9121 agctggcacg agatattggc acggtgatgg tgggtaagga tgccatcgaa gatggcttaa
     9181 tcgatgaagt gggaggcctg ggtgctgcta ttgccaaact gcagtcatta attgatgggc
     9241 attatccaag tgaggtgatc cactaatgct gtggactgtc atgccgctgg aaatggtcat
     9301 ggagggaagc gacagttttg cacctacata caccgagatt tcctggcagg aaagtacctt
     9361 agtagttgaa ccgttggaga tgaacaccgc cagagtggta agaatcatct ctactaaccc
     9421 ggaagactat ttgaacccac aggtacagcc aggcagcatc attcaaatca gaaacaacca
     9481 gcctgacctt tcataggtca ggctatttgt ttctagacaa ttagttgcag atagttttct
     9541 agccgacaaa ctagcagcga acatcattag gctaagaaaa gcacgaaagg tgtaataatt
     9601 tgctatggat gtaaatagaa gaaaataaag gaaatagctg ctatggtgtt taaaatagat
     9661 ataaagaaag ctaaaggtga taagggggaa aactgcgtgc gtggcaaagc gttgttgatt
     9721 ctattgtcga tgctgttagt cttgactggt tgtgcagcac cattttcaga ggtacaggtt
     9781 gctgaggaaa tcgcggcatg ctaccaactg ctatcaccaa cagtagtaac cattaatttg
     9841 ttagcaacat taccgcatca tggcaattta ctggtgttag tagcagatca tagtgctgca
     9901 gaggcacctt tgatggattt attcttatta aacaccaagg gggtggtatc cttagcttca
     9961 gcaccactga caaacaagat aaaactgcac agtttagtag atggcaatga aacgctcctt
    10021 tatggccctt tgccaacggc agcagaagat ggtattcctg tcgagaattt gccggacgct
    10081 atagataccc tgcaaactat cactggtaag gcagccatca ctttgaatag ttggaagatt
    10141 attgaacagc aactaccaat agcaaaaggt tttatcctag ctttaaagca aaaaagacag
    10201 gcagaagcag tcaaccttta tgatgaaacg gggaaaatac tggcacagtt tgccaagtta
    10261 agcaaaggag caatgaaaga aacaagtttt actgcctggg atgaactgcc tcttcctcgg
    10321 gaactagagc aggaaaatat ggccgcagtg caagttgcag cctcagtctt ggcagcagaa
    10381 gatgaaggcg ttagtttagc agaggaagag gtgcgtgagc ctctgatcgc agcctttaac
    10441 gcttgtttgc cagaattaag gagaaagggc agcgaagctg cagacactaa ccttgtggta
    10501 actattcact taaatgatgg aagctctgta ctcatcaaac aacataacag caactttacg
    10561 atttcttttg agcgtgctga agttaccctt aactatgagg ctagttcttc agagttagca
    10621 gctttgttta aaacatacag taaattgttg aaattataac ttgattattg ataaacaaaa
    10681 cgaagatcag cctgagatag tctatggtac aatttaaatt catcttgctg tgctcttttt
    10741 gtgataaaaa tatcggtaaa gaaatcccac aaaaataccc aaccaccgat aaatatgcct
    10801 tcctggagga cattaaataa aatacctgta actttgcttc cccaaaagta gccgacggtt
    10861 aagaacagta cggccaacag taaggaggta actatttttc gatttagttt gttcatagtt
    10921 ttagcaagtc tagcttgagc ataatcataa taattatggt aggcggaaat cacagcttgc
    10981 tccttttttt catcttgctt ggcaagaggt aaatagagca ctatactaag ctggtaggca
    11041 agcgggatgt cctcagatga attataaata aagtcatgaa attcatcctc aatgtcccgt
    11101 cgtttgaagg gtgagggatc ccagctatca taaacatcat tatagctatc caaggaaact
    11161 tcaattaaat aatgttgact ttccttttca aatttatata agttctgaaa atatcttttt
    11221 tttctcatct gccaccctaa tttctattct atct
//
LOCUS       DASRMS010000003         2015 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_872772, whole genome shotgun sequence.
ACCESSION   DASRMS010000003 DASRMS010000000
VERSION     DASRMS010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872772"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="VFC74_00090"
     CDS             <1..263
                     /locus_tag="VFC74_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23774.1"
                     /translation="IILFTLLAFSYLSAHANQIPRYNPEAYCQQVANFSGGSSMILNG
                     CIDMEQQSYNNLKRIWASIPNRTQNYCEEVASTTPYVTLVVR"
     gene            313..1865
                     /locus_tag="VFC74_00095"
                     /pseudo
     CDS             313..1865
                     /locus_tag="VFC74_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          607 a          465 c          469 g          474 t
ORIGIN      
        1 gaatcatatt atttacgctt ttggcttttt cttacctcag cgctcatgcc aatcagatac
       61 ctaggtacaa tcccgaagca tattgccaac aggttgctaa cttttctggt ggctcgtcaa
      121 tgatattaaa tggttgcatt gatatggagc agcaaagcta taacaatctt aagagaatct
      181 gggcttcaat tccaaatcgt acccagaatt attgtgagga ggtggcctcc actaccccct
      241 acgtcacact agttgtccgc taatctgatt ttacctttat actaattaac ttggggcggt
      301 caggagtgtt agatgccgtc ttatagccca gaattcaaag agcaggcagt taaaaaaatg
      361 atgccacctc aaaatcatcg tgtctctgac ctcagtgttg agcttagtgt ggctccagca
      421 actttgtacc gatggaaaaa tgaataccgt gccaaaggat ttgtagtgcc gtcaaaaaag
      481 actttacccg ataagtggga ctctaaagcc aagcttgcag ccgtgattaa aaccgctgcc
      541 atgaatgagg cagaacgttc tgaatactgc cgcgccagcg gcttatatgt tgagcagctt
      601 gatgagtggc gtgagtcgtt tgaatcaatg ggagcttctt ccagtgctaa agataaggcg
      661 gaattggcac gtgagcgcaa aaggaataaa aagcttgaaa gagagctcaa acgcaaggac
      721 agggctttag ctgaaacagc agccttgctg gtgttgtcaa aaaaagcgca ggccatctgg
      781 ggcatagaag aggaagatta attcctctta agatgaagca gatggccata actcttataa
      841 atgaagcagt gcacacgggt gcaaggcaga ccaaggcttg tgaggtcatt gggattactt
      901 gtcgtacgct gcgccgctgg cgcagcgtac acgacctgac cgataagcgc acctgcccac
      961 aaccacgcgc ttatccacat gctctgacgg cagaagaaaa agacctgata ctagacatta
     1021 gcaacagcgc acggtttaaa agcctgccac cgtcacaaat agtgcctatg cttgccgatg
     1081 agggcaagta catagcttct gaatcaagct tttaccgtgt gctaaaggct aataatcagc
     1141 taaaccgtcg cggccacgca cttcccccaa aggagaatct ggtgccagca gcattaaagg
     1201 ctactgcttc aaaccaggtg tggagctggg atataacttt tttacccagc gccgtgcggg
     1261 gtcagttcct gcgtttatac atgataatgg acgtatacag ccgttatata gttggctggg
     1321 aagtgcagca caatgagtcc tctgagcatg cagcaagcct gattgataaa gcctgcctaa
     1381 aacatcaaat caataaaaat caacttgtgc tgcattccga taacggcagc cccatgaagg
     1441 gggccaccat gctatctacg ctgcaacggt taggcatagt gccgtcattt agtagaccat
     1501 cggtaagtga tgataatcct tactcggaat cactatttag gacacttaaa tataccccag
     1561 cctatcctag caaaccattt aatgatattg atgccgcaag gcaatgggtg catacctttg
     1621 ttagttggta caacaccacg caccgccata gtgctattgg atttgtaacc cctgagcagc
     1681 gtcacaatgg tctggatgtt gcaatactta ataggcgcaa ggcggtgtat gagtatgccc
     1741 gacttgctaa accacagcgc tggaaaggcc ggaaaacgcg taactggaac cactgtgata
     1801 cagtgctact aaacccacta aaagagcatg taaaaacgga gaaaaacctg aaaattgctg
     1861 catagaaaac cggacaacta gcgagaaaga caccggctta ctaaaccttg acctatcccc
     1921 caaaaacagt gccataaggt tgatgagatt tccattaaac tgggacaaaa ggagatctca
     1981 ccatgaagaa gcgttacagc gaagaacaaa ttatc
//
LOCUS       DASRMS010000004        28724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_537374, whole genome shotgun sequence.
ACCESSION   DASRMS010000004 DASRMS010000000
VERSION     DASRMS010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 28724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28724
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_537374"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VFC74_00100"
     CDS             <1..311
                     /locus_tag="VFC74_00100"
                     /inference="COORDINATES: protein motif:HMM:NF039975.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6063 family protein"
                     /protein_id="HZK23775.1"
                     /translation="FTSSQDKAVDEETGLAITDIAKYWFALTNEDDAAVFKTRRWYIF
                     QVMNFLKKQDLISIQDETVIIPTAKFNRLAANYFLNYERLDDIKRILSEKRGEEDSHA
                     "
     gene            304..4632
                     /locus_tag="VFC74_00105"
     CDS             304..4632
                     /locus_tag="VFC74_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23776.1"
                     /translation="MPKLSKTRIINLNYNDGKRTIYNEVFDYGNGKDTLFSMDNGIGK
                     TVLIQFFMQPFIRNKRELQGRKFDDYFSGNAPTYIMHEVLLDNGENLLVGMLIKKDST
                     DDGRNKLKILAFTNRYAQANSFDIINAPFVEDKRILNFSESEERIKKYQSGKLNFKYY
                     NFNDSSKKTAYFEDLKSYKLDYKEWEDIIRNINNDESGLSNLYDKHKTSEALLKNVIV
                     PLIESKLNGEKNIIASIRSNVSKYIESYKRSKDSFFEIKLLKEFTGDLAPVNQLLQEG
                     VLLEQQREALEQKLANIALLYQKVLAAKVAEKLHHEELREYLQSELTEVAYEEHSLNY
                     YKLHDQEERQLQKLKDMKEEFHKQEEKKQELVKEKYIQECAEAYGELCLIEADLTEIR
                     ERIANYEKDDSEIARNIKNYQFSLKHQYGLELEKLRKIEGAFLKKQADEESELKEIAA
                     AIKRNDLQQKEAIRLEESYKNSINNFAKLEASFQQKYPDFVVIRNILLAEYDETELHS
                     YRLSIAESMKSTSQLEEKLNKEAAFLQRTRTLLAESEQEKSNLLTELKIELASQEKDL
                     QVFQQETEKIAVILRIKNLPPDPVAQRGKLLQRLESENRQLQNDLTKQNDQLREVQDT
                     MHRYETGLIQLPEDVLQCFANKGIEFQYALNWLQNYQGSREEKESIVNHNLFFPYGII
                     LSEKDIDLLKNESIDVFTSIPIPIINLNELNNQPDTDQHNGVLTIQNQDFLIAFNYLL
                     IDEEERAALLTKLAYEKSRLVSVIEDIQNAINRNQEYEKTVLDYPYHGDEGHDMAAKI
                     SSLIQNIAEITVQLAEQSEEIKSSIQRSEAINSELRNNEQQQFLLREKERQFDKFISE
                     HRELKKNNQALLDVQHHLKNLNKEEIKLSEAKAALEDSLKGLVLEIHDIELALKSCLA
                     DLELYKNVDSGMLLAEDKDALEAKLKACEEKLNKDIKRDKDEARKLNDNLINTNKRLK
                     KKANEGNILPADYVGVNYYELVLTDLENSISKLETQMKLLNGQIHNLDTAVKIMANDK
                     QHELKDIQKLGFAVPIAREKIKDPNFKARENKISEDLQQNEIIIGEYLEQISSLSFLK
                     YKLEPFTDYVQQTDDFAFDFSHIPAAIKSINANLSSADAVRKQISQVEENLTRMINRI
                     YENYRDKDRFLKDRLSDYQSKTSKIRNHNEIESLLEVVGRKINTLEMALKHIHEEEEV
                     VIDEILRYANNVLVELKTIDKKSSIKHAGKTQKLLEISIPEDREEESLKEYIKDKVSY
                     YANLEEDYTNLLDHDIQSTELLSKLVGNITRIRIDIKKIERARLVRKSWSEALTQNSG
                     GERFVSMFILLSSLMSYMRRRATDIDNREEKKILIMDNPFAKTHTEHLLEPMFQIAEK
                     YNIQLICFSGIGGSAIYNRFNKIYVAKVIEDRFRNKEHVSFKAGNEETLELSDFTITK
                     QQLSMF"
     gene            4795..5535
                     /locus_tag="VFC74_00110"
     CDS             4795..5535
                     /locus_tag="VFC74_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK23777.1"
                     /translation="MNEVLKQLKNRKSVRVFEDKPIAVELKEEILGAAFEAPTAGAMM
                     LYSIIDVTDDILKQELAIVCDDQPFIAKAPLVLVFVADYQRWYDAYGVAGCEVRQPGE
                     GDILLACADALIAAQNTVVAAEALGLGSCYIGDIMENCEKVRELLALPDYVLPAAMVV
                     YGYPTAKQLKREKPARFNQQYLVFENQYRKLSAKEHLAMQEERLKKRGLNDVDGKEEI
                     RAVCLRKYLSDFTTEMNRSVGEYLKKFR"
     gene            complement(5736..6107)
                     /locus_tag="VFC74_00115"
     CDS             complement(5736..6107)
                     /locus_tag="VFC74_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23778.1"
                     /translation="MEIKEHLIIPLGYGKFVRSDKIIALEPIEENRGPGRRTKVFIEQ
                     MPNPVIASRTEQAIIANIVETPLEIINATAALELLYDLLDDISQVGPMLRNSIKQEAD
                     LDLDKLELKIKDVLEQRLDDE"
     gene            6556..7419
                     /locus_tag="VFC74_00120"
     CDS             6556..7419
                     /locus_tag="VFC74_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015614666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK23779.1"
                     /translation="MKDVITVDQLSKSYGNLNVIDKISFSVSEGEIFALLGANGAGKT
                     TTLECIEGIRSYDAGSITIDGTIGVQLQSSSLPANITVLEAYKLFCKWNHVREDPELL
                     DTFGLALLKNKQYEAMSTGQKRRLHLVLALINNPAIIFLDEPTAGLDVEGRVALHTEI
                     RKLKEQGKTIIMASHDMAEVESLCDRIAILKGGDIVFIGSSTELTSRYVGESKIFIKT
                     QLPITRDDFKHSTYLATHEGYASVRTVSIGNALLELLNEAKENGNEVLDIKIERASLE
                     QRFVEIATEGK"
     gene            7422..8138
                     /locus_tag="VFC74_00125"
     CDS             7422..8138
                     /locus_tag="VFC74_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK23780.1"
                     /translation="MSAFLYGVILQWKLDLRNKGVILTYYVVPLLFFAFMGAIFTSIN
                     PTAKDSLIQSMTIFGTTMGAFLGTPTPLVELYGSDIVKAYKVGGIPVWMAAVNNFISA
                     FFHLFIMSIIIYLLAPMAFAAKVPQSTLYFFLSLAFFIVVCLAIGSVLGLFIKDISKL
                     TMFSQLIFLPSLMLSGIMFPVDLLPIVLQNVGKVFPATWALMILGANEFDFTLFLPLL
                     FILCLAVFASVYKLSRVGIE"
     gene            8501..9205
                     /locus_tag="VFC74_00130"
     CDS             8501..9205
                     /locus_tag="VFC74_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23781.1"
                     /translation="MKKDVLICDDQQVFIEEFKQRHGQYYEIREVNDVGMLVDKLHDM
                     KHLPDLLLLDLYHPRAQGEDFEQKRQVAEQELANLDEQIKITKTAVEAAWNPVGLEIL
                     KDIRQDFSAEALPVIIYSQRGLFLLDDEKVRLVDEYDAHWLLKRQFGPITEKTRMDRV
                     MRHEQKTTSLPKIYNRALTISWSITAIVLAVYVLPIDSLLWQIAVGVLGGLATNVLAN
                     YYNKLMKPWQNKNSHR"
     gene            9228..9896
                     /locus_tag="VFC74_00135"
     CDS             9228..9896
                     /locus_tag="VFC74_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK23782.1"
                     /translation="MKKRILICDDQARFITEFKKRFSQDYEIEVVDDVRQVMAALQSG
                     SQLPDLLMLDLYHPLVVDAEFEEHRQAAERELARLAEQVKKAKTAVELTWSPLGAQVL
                     QELREHYGAEELPVMIYSQSGLFVLDEEKVRAIEENDGEWLLKKQFGPLTEKTRMERV
                     MRTARRGRLVKKYQLLVGVSWAVTLFMVSLHFFPFTAVVWHLLVGVGGGLLSYIWIGR
                     FQRV"
     regulatory      10000..10090
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            10132..10830
                     /locus_tag="VFC74_00140"
     CDS             10132..10830
                     /locus_tag="VFC74_00140"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HZK23783.1"
                     /translation="MLGEHFLGKIDVEADLPYEISKNFEKSIRRVFDTKEEQIVEFAY
                     QIAEGKTLHYYTRWVPEFNDEGLVKSVLCIARDITKRKETELRLLAGDVTERKTIEEE
                     NYRMRETLENVVSSGAVGIRIVDRNYSVIWSNESSEVVYFSINDDQPCEIFSYAGGTC
                     LSHLDCDLAGQLGKIFSEKKEIVKWEQQVIRNGKLVWLRVIATPIHNKLLEVESVALF
                     LIPITELKSALVKL"
     gene            10843..12186
                     /locus_tag="VFC74_00145"
                     /pseudo
     CDS             10843..12186
                     /locus_tag="VFC74_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            12220..12390
                     /locus_tag="VFC74_00150"
     CDS             12220..12390
                     /locus_tag="VFC74_00150"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0E family sporulation regulatory
                     protein-aspartic acid phosphatase"
                     /protein_id="HZK23784.1"
                     /translation="MKPAIFSNQLDLERNRLHQIAEEKGITSIEAIEQSTKVDHIINQ
                     INQLETIRKMSK"
     gene            12692..13306
                     /locus_tag="VFC74_00155"
     CDS             12692..13306
                     /locus_tag="VFC74_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK23785.1"
                     /translation="MELLQVQNVKKVYKTRLGAQKFTALQNVSLEVAEGEFVAIMGAS
                     GSGKTTLLNIIASLDNLRRAMYFQQVTGRAETLLAKEILLLTNEQLAEVPAHVKKVIA
                     DTKFTTGKNGTPHRAIFLVAADRAQAEAWYQKLAPEGIGIRIKGNLVLNIAGEGEQPL
                     TFSNELRSQASTIENVIWTADIYSERVEGYGIFGRCHAAYFSRQ"
     gene            complement(13668..13985)
                     /locus_tag="VFC74_00160"
     CDS             complement(13668..13985)
                     /locus_tag="VFC74_00160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HZK23786.1"
                     /translation="MGKKSKYKVIISDRSRQMLADHVRFLAQKSPAAARKTKNELLAA
                     LRSLSTMPERFPFLNAEFLPLNKYHKMFVEKWYLILYQIKDQTVYVDYIVDCRQDYGW
                     LIS"
     gene            complement(13969..14253)
                     /locus_tag="VFC74_00165"
     CDS             complement(13969..14253)
                     /locus_tag="VFC74_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZK23787.1"
                     /translation="MRIKPSASIRQNYNEIAALCKSSGEPVYLTKNGEGDLVVMDIDA
                     FTRREKMLKLREELLAVEEDRLAGRSGVTPDDLDSSLDSIIDEVEHGEKK"
     gene            14467..14613
                     /locus_tag="VFC74_00170"
     CDS             14467..14613
                     /locus_tag="VFC74_00170"
                     /inference="COORDINATES: protein motif:HMM:NF013077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK23788.1"
                     /translation="MKEVLKQLKNRKSVRVFEDKPIAAEMKDEILGAAFEAPTAGAMI
                     LLQR"
     gene            14820..15983
                     /locus_tag="VFC74_00175"
     CDS             14820..15983
                     /locus_tag="VFC74_00175"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK23789.1"
                     /translation="MKTEELLQYRRQLIEDTVRLKKRERVPHFSNICLWLVLDYGCRL
                     TEACSDWQLMEKCQLAFFNKYKFDFAQTIAGLLCHPMAMIKDIGQGFYFYNDVNSTVS
                     LRDMRLLLPNEYEQFIADLPRYTWEVLLPRKYERWPELKVKDLLEPLAEYQRYTDFAT
                     RMGEAAVTRYSIPRASAEMVGMPGIEMLCNLYTGMKGLAIDMRRQPEKVKAAVQMLES
                     GTDAVVQRILEEPRQNNVAAFDLWLGMLAHNFLSIKQWDAYYWPWLKKVLDAVVAADK
                     TAYIFVEGYVSRFYDYFRVFPAGHIALHIEQDDVYEVKKNLPNCCVVGGMSSTLLGSG
                     SKEQCIARAKQLIDELGKDGGFILSQDKMISYMRDADPANLKAVCDFVSEYYI"
     gene            15998..16150
                     /locus_tag="VFC74_00180"
     CDS             15998..16150
                     /locus_tag="VFC74_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23790.1"
                     /translation="MEKKDINLELSLANYEEAVAEISKLLDFLPADKAVRKEYVDAIA
                     NKLLWL"
     gene            complement(16598..17743)
                     /locus_tag="VFC74_00185"
     CDS             complement(16598..17743)
                     /locus_tag="VFC74_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK23791.1"
                     /translation="MSSLQAERNQLFADIWEGRKPKRVPVTAMISLEYAIQHQGYSLF
                     REYYSAKHCFEVAEEMAKLINSDTLPTHPENSAAAFRYSLSKFMVQGRDGFYQHPNYS
                     PMKFEEYPDFIKDPFEYWANVVRPRIFGILEEDERYGELRLNIARNVVASKSWGMGTP
                     QLVEKYERSNIEAMAMMSCEPLDYIADFLRNFSDICIDMRRAPEWVCDAAEAMLQFSC
                     DSLDRVTKVPGKINKVTLPLHMAPFLNAKNFEKFYFPTFKKLIEAIQERGLHASIYCE
                     HDWTPHLEALNDLPGRVEIGFEMADPKLVVEKLDKRHIISTFFKTHMLRTATPEQVSD
                     EVKRLLDIVAVNGNYIFAMNKPVLNPGDAKIENIQAMVDTAVEYGVY"
     gene            complement(18006..18722)
                     /gene="prxU"
                     /locus_tag="VFC74_00190"
     CDS             complement(18006..18722)
                     /gene="prxU"
                     /locus_tag="VFC74_00190"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084691462.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(18477..18479),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HZK23792.1"
                     /translation="MPEEFKPGCQRPAIKKAAPPAANAAIAPIKEEHTMLPKVGKPAP
                     DFAAPGFFQGKFMNFDLSEFKGKWVLLCFYPGDFTFVUATEISAVAEKHEELKALDVE
                     VLSVSVDSIYVHKMWNEHELSKMVNKDIPFPMLSDQDGSIGKMYGIYNEDAGVETRGR
                     FIIDPDGIIQGFEVLTPSVGRNVAESLRQIKAFQLVRASKGTEATPSGWQPGKDTLKP
                     NPDLVGNVWKVWKVSQAFDE"
     gene            19674..20912
                     /locus_tag="VFC74_00195"
     CDS             19674..20912
                     /locus_tag="VFC74_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZK23793.1"
                     /translation="MSPMLIGVDVSLRSHHVQFMDGAGRSLASFSVPNDQSGADTLIH
                     KMLDTASKEKLKHLHIGMEATSNLGWHLAHYLKSELQNYEPNVMSQVYVLNARKVARF
                     KKGYDTLPKNDRIDAWVIADHLRFGRLPHEMKDIIQYEALQRLTRTRFHLMKNIKRDK
                     TYFLNQVFLKFSGMRQDNPFSNTFGSTSLAVIQELEPDQIMAMSMEELIEFLQDKGKN
                     RFENPEEIATYLKKIARSSYRLDKAMADPVNISLATTLSVIQHLESEVKKLDKEIAKI
                     MKGIPQTLTSVKGIGPVYAAGIMAEVGGIDRFTNHADLAKYAGLVWNQNQSGEFEAEE
                     TSRMRTGNKYLRYYLVQAADSVRKHDTEYAAFYQKKYDEVPKHKHKRALVLSARKLVR
                     LVFMLLKTNTLYTPPERRNP"
     gene            21317..21460
                     /locus_tag="VFC74_00200"
     CDS             21317..21460
                     /locus_tag="VFC74_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23794.1"
                     /translation="MKRFLLVEDEQIMAKNIAFFWSGKGIGLILLMMVKQRWSCLPTR
                     NMI"
     gene            21433..21996
                     /locus_tag="VFC74_00205"
     CDS             21433..21996
                     /locus_tag="VFC74_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK23795.1"
                     /translation="MFANQKYDLVLLDWTLPKLDGLEVCKQIRQASRVPIIMLTAKGE
                     ILDKVIGLEVGADDYLVKPFDQRELLARVHVLLRRLPAGLAADSWLQYEELRLDREKL
                     LLVCGEEAAALTANEYKLLELMMKKPHNVFARTYLYEEVWGEMPGYNDRTVDVTISRL
                     RKKLLELSGKKYFQAVRGLGYRLGEQP"
     gene            21993..23354
                     /locus_tag="VFC74_00210"
     CDS             21993..23354
                     /locus_tag="VFC74_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK23796.1"
                     /translation="MKIQSQLWLILSVLFLVMSLVVYLVVADAYEARLQTSYQQISFA
                     QGSALLDELTKAYPDSRQRSSGYLQNYSEQLQARLILLTEQKKVFADGFAVLEANSAL
                     DLTVLKAGTGYASHFAQTEAFGYVQYTLLPFDKASSKGYLLIIQEAGGLSAELSSFRA
                     WMLRLLVLALLSFFVLSYVVAAWLTKPMRRIIASLKQITPHKRDFALTYRRQDEIREL
                     IAAIGRMVEELNLYDERQRRFVSTSSHELKTPLATIQLILENLPYVRENEETYQEFTE
                     DLVFQVKKMRQIVEQLLQIMRLGDSPLQKETVSEEDIRQHLLQVFQYLVQEKELTWEF
                     ELQKTSFNVDRLLFLRALDNLVANAIRYSPQGKTIKIVVKGDKIASVVSVCDQGIGIA
                     AADLPQIFEPFYRANDATAYNQEGSGLGLAIVKQIAELHQGKITVNSVPQEGSCFHFL
                     LPT"
     gene            23449..24426
                     /locus_tag="VFC74_00215"
     CDS             23449..24426
                     /locus_tag="VFC74_00215"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HZK23797.1"
                     /translation="MKKLLTSIGIILFSLLLVVGCAKNNATPPKNDEQEPPAVVDPDD
                     NQEGPIVIDPAEPTEEPLDENDLTPTPEDKEQVHQIVLYFPDSDLLRTYRVKTQITLK
                     EHEQLPKAALEAWLAGPRDKKLTGLADEAVVIEYVKDVKGVAQVSFSKEVQDLNVGFE
                     GELLFAEQVAMIMQQFGYEQTQLLLEGKVVETLFGHLYTKEPMMAKAPADYRWVEEMD
                     TRKFVLQNTAFRIYEPAPGTVVKDRIVIRGLARVFEANFLYEFEDGHNILAKGYTTAS
                     EGAPGWGEFTIVIEIDEVANNSGTIIIYEASAKDGSRMNELRIPVTVAK"
     gene            24616..25995
                     /locus_tag="VFC74_00220"
     CDS             24616..25995
                     /locus_tag="VFC74_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK23798.1"
                     /translation="MKEDVKKLQQERGKLFSDFYNNKIPSRMFVSVSVGSHILAEFGG
                     LNDFEVQYDYSLLAEPAKKICEMIYSDTSPVSPVGIISRPPSFYQLLGSQSFVMGNRG
                     FVQHPEVVGMHEDEYPELIADPYSFLLEKVIPRQYKELDASNPVRMLNRMQMAKSALA
                     HDSASAMPWFLPLAKEYGYYQGAPRGSGGFTEAPYDFIADQLRSFSGISKDIRRHREL
                     LKEACDAVLPLMFRMGLPANPHPEGAVGTPLHMPTFMREKDFMEVWLPSYKKMYENYA
                     ALGIRGSAFCEDDWMRYLDNLLELPAGMRLAFEYGDPQQIKDKLGKKFILSGLYPLDL
                     IKHGTKEECVYKVKELLDIMMPGGGYVFGFDKGPLTLSDVNLENLAAVAETVRDYGVY
                     DNAGESFGTPVNSEGFVFDESIMKLDSKYFFNWDEFKAKYPLTPDSARANFEKYDKEL
                     FDQYMLMLI"
     gene            26396..27775
                     /locus_tag="VFC74_00225"
     CDS             26396..27775
                     /locus_tag="VFC74_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK23799.1"
                     /translation="MKEEVKKLQQERAKLFSDFYNNKIPTRQTVSMSVGNFILAEFGG
                     LNVFEIQYDYSKLAEPARKICEMIYSDTSPVMPASIISRTPSFYQLLGSQSFVMGNRG
                     FVQHPEVVGMYEDEYPELLEDPYAFLLEKVIPRQYKELDATNPARMLNRMQMAKSSLT
                     HDANSCMSWFLPLQEEYGYHRGAPMGSGGFCEAPYDFIADQLRSFSGISKDIRRNREV
                     LKEACDAVLPLMFRMGLPANPHPEGGVGTPLHMPTFMREKDFMEVWMPSYKKMLENFA
                     ALGVRVNAFCEDDWMRYLDNLLELPAGTKLMFEYGDPQKIKDKLGKKFMLQGLYPIDL
                     IKHGTKEECVYKAKELLDIMMPGGGYIFGFDKGALTFSDLNLENLAAVAETVRDYGVY
                     DNPGESFGTPLNSEGFVFDESIKKLDSKYLFNWDEFKAKYPLTPESARANFEKYDKEL
                     FAQYMLMLI"
     gene            complement(27846..>28724)
                     /locus_tag="VFC74_00230"
     CDS             complement(27846..>28724)
                     /locus_tag="VFC74_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23800.1"
                     /translation="DFDHLYRYANLLELDKNIPAQKLVKEYVEFTPGRPTIAEHRHPS
                     DTVRGYINFKSADIRTKLNIMIITAAEQQTMNFYMNLGPTYYNDLGRQLYQEIALIEE
                     EHVSQYGALMDPNCTWLENLLMHEYMECYLYYSFYQDERDPHVKDIWEMHLRQEIAQL
                     HRAKDLLAQHEHKDWQQVIPGGVFPKLLQFHDTKDYVRKILGQQVELTADQEVLKNVN
                     DLPDNHRFFWYQQTVNHDLKAVPSHAVIAQHQQKKGQDYRYEDKPNAVEALRDRRQDN
                     VKIARTKQKQRQLVGV"
BASE COUNT         8554 a         5410 c         6769 g         7991 t
ORIGIN      
        1 agtttacatc tagtcaagat aaagctgtag acgaagaaac tggtttagcc attacagaca
       61 ttgccaagta ctggtttgcc ttaacgaacg aagatgatgc tgctgtcttt aaaacaagaa
      121 gatggtatat ttttcaggtc atgaactttc taaagaaaca agatttaatc agcattcaag
      181 atgaaacagt aattattccg accgccaagt ttaaccgtct cgccgccaat tatttcttga
      241 attacgaaag gcttgacgat attaagagaa tcctttctga aaaaaggggt gaggaagact
      301 cccatgccta agttgagcaa aacaaggatt atcaacctta attataatga tggtaaaaga
      361 actatttaca atgaagtgtt tgattatggc aacggcaagg atactctttt ctccatggat
      421 aacggcatag gaaaaacagt gctgattcag ttttttatgc aacccttcat tcgcaacaaa
      481 agagaactgc agggtaggaa gtttgatgat tacttttctg gtaatgcccc aacctatatt
      541 atgcacgagg tacttttaga taatggagaa aacctgttag tcggcatgct gattaaaaaa
      601 gacagcactg atgacggcag aaataagctt aagatcttgg cctttaccaa taggtatgct
      661 caagcaaata gctttgacat tatcaatgca ccttttgtgg aagacaaaag aattttaaat
      721 ttttctgaaa gtgaagaaag aattaagaaa tatcaaagcg gtaagcttaa ctttaaatac
      781 tataatttta atgacagcag caaaaaaacg gcatacttcg aggatctcaa gtcctataaa
      841 ctggattata aagagtggga agatatcatt agaaacatta ataatgatga gtccggtctg
      901 tctaaccttt atgataagca taaaacgtcg gaagctttgc tcaagaatgt catcgtgccc
      961 ctgattgaaa gtaaattaaa tggtgagaag aatatcattg cctcaattag aagcaatgta
     1021 agtaagtata ttgaaagcta taaaagaagt aaagattcct tcttcgagat aaaactgctc
     1081 aaggagttca caggggactt ggcgcctgtg aatcagcttt tgcaggaagg cgtcttgctg
     1141 gagcagcaaa gggaggcctt agagcaaaag cttgctaata ttgccttgct gtatcaaaag
     1201 gtcttagctg ctaaagttgc agaaaagctt caccacgagg aattacggga atacttgcaa
     1261 agtgagctta ctgaggtggc ctatgaggag cactctctaa actactataa actacatgac
     1321 caagaagagc ggcagctgca aaagctaaag gacatgaaag aggaatttca caagcaggaa
     1381 gaaaaaaagc aggagctggt taaggaaaaa tatatccaag aatgtgcgga agcctacggg
     1441 gagctttgtc tgattgaggc cgacctgacg gaaataagag aacgaattgc taattatgaa
     1501 aaggacgatt cagaaatcgc ccgcaacatc aaaaactatc agttcagctt aaagcaccaa
     1561 tatgggttgg aactagaaaa attacggaaa atagaagggg ccttccttaa gaagcaggca
     1621 gacgaagaaa gtgagcttaa ggaaattgcc gcagcgatta agcgtaacga cttacagcag
     1681 aaagaagcga tcagacttga ggagtcttat aaaaatagta tcaataactt cgcgaagctc
     1741 gaggcatctt tccaacaaaa gtatccggat tttgtcgtaa ttagaaacat tctcttggct
     1801 gagtacgatg aaaccgagct tcatagttac cgcttaagta ttgccgagag tatgaagtct
     1861 acatcacaat tagaggaaaa gttaaacaaa gaagctgctt tcttacaaag gacaagaacg
     1921 ctattagctg aaagcgagca agaaaagagt aatctcttaa cggagctgaa gatagagctt
     1981 gccagccaag agaaagatct acaagtcttt cagcaagaaa ctgaaaaaat agcagtaatc
     2041 ttacgtatca aaaacttacc tcctgaccca gttgcacagc gaggaaagct tttgcagcgg
     2101 ctagaaagtg aaaatcgtca gctgcaaaat gacttgacta aacaaaatga tcaactgcgg
     2161 gaagttcaag ataccatgca caggtatgaa acgggcttaa tccaattacc cgaagatgtc
     2221 ctgcaatgct ttgccaacaa gggcattgag tttcagtatg cgctcaactg gctgcaaaac
     2281 tatcaggggt ccagagaaga aaaggaaagt atcgtgaatc ataacctctt tttcccttat
     2341 ggcatcatct taagtgaaaa agacatcgac ctcctgaaaa atgagagtat agatgtcttt
     2401 acctccattc ccatccccat cataaattta aatgagctaa ataatcagcc agacactgat
     2461 caacataatg gcgttttgac gatccaaaac caagactttc tcatagcttt taattatctg
     2521 ctgatagatg aagaggaaag agcagcacta ttaaccaaac ttgcatatga gaaaagtagg
     2581 ttggtgtctg taattgagga tattcaaaac gctatcaaca ggaatcaaga gtacgaaaaa
     2641 acagtgctcg attatcctta tcatggtgat gaaggtcatg acatggccgc aaagataagc
     2701 agcttaatac aaaatattgc agagatcact gtgcaattag cagagcaaag tgaggaaata
     2761 aagagcagca tacaaagaag tgaagcaatc aatagtgagc tgcgaaataa tgagcagcag
     2821 cagtttctcc tcagggagaa agagcgtcag tttgataaat ttatcagtga acaccgggaa
     2881 ctcaagaaaa ataaccaggc acttttagat gtgcaacatc acctgaaaaa cctcaacaag
     2941 gaagaaataa agttaagtga agctaaagca gcactggaag atagtcttaa agggttagta
     3001 ctagaaattc atgatatcga gttagccctc aaaagctgtt tggctgactt ggagttatat
     3061 aaaaatgtag actctggtat gttgctggca gaagataagg atgcccttga agccaagctg
     3121 aaggcctgtg aggaaaagct gaacaaagat attaaaagag ataaagacga ggcaagaaag
     3181 ttaaatgata acctgataaa cactaacaag aggctgaaga aaaaggccaa tgaaggtaac
     3241 attttgcccg cagactatgt tggtgttaat tattatgagc ttgtcttaac ggatttggaa
     3301 aacagcatta gcaaattaga gactcagatg aaactactaa atggccagat acacaattta
     3361 gatacagcag tgaaaatcat ggcgaatgac aaacaacatg aacttaaaga tatacaaaag
     3421 ctgggctttg ctgtgccgat cgccagggag aagattaagg atcccaactt taaagcaaga
     3481 gaaaataaaa tcagtgagga tttacagcaa aacgaaataa ttattggcga gtaccttgaa
     3541 caaattagta gtttaagctt cttaaagtat aaactggaac ctttcaccga ctacgtacaa
     3601 caaacagatg attttgcttt tgattttagc cacatcccag ctgctatcaa gagcataaat
     3661 gctaacctct cgtcagcgga tgctgtgcga aaacaaatct ctcaggtaga ggaaaacctc
     3721 actagaatga tcaatagaat atatgaaaac taccgggata aggacaggtt ccttaaagat
     3781 cgccttagtg actaccaaag caaaacaagt aagattagaa atcataacga aatagagtcc
     3841 ctgctcgaag tagtaggacg aaaaataaac accttagaaa tggctttaaa acatattcac
     3901 gaggaggaag aggtagtaat agatgaaatc ttgagatatg ccaataatgt gcttgtggag
     3961 ctcaaaacaa tagataaaaa gtctagcatt aaacatgcag gcaaaaccca aaagctccta
     4021 gaaatcagca ttcctgaaga tagagaagaa gagtctctaa aagagtacat caaagataag
     4081 gttagctatt atgctaatct ggaagaggat tacaccaacc tgctggatca tgatatccaa
     4141 agtactgagc tgcttagtaa actagtaggc aacattacca gaatcagaat agacattaag
     4201 aaaattgagc gagcaagact ggtaaggaaa agttggagtg aagccctaac ccaaaactct
     4261 ggtggagaaa gattcgtctc tatgtttatc cttctttctt ccttaatgtc ttatatgcgc
     4321 aggcgggcaa cagacattga taacagggaa gaaaaaaaga ttttaattat ggacaatcct
     4381 tttgctaaaa cacatacaga gcatttgtta gagccaatgt tccagattgc agaaaaatac
     4441 aacatccaac taatctgttt ttccggcatt ggtggtagtg ctatatataa tagatttaat
     4501 aaaatctacg tggctaaggt cattgaagat agattcagaa ataaagaaca tgtatctttc
     4561 aaggcaggta atgaagaaac cctggagctg tcagacttta caatcactaa acagcaatta
     4621 tcaatgtttt agacagcctg cagttttgca tataacaata cacatgaata tgcatctctt
     4681 tagaagtagg attacctgat tctttagcaa atggctgcaa ctcgtcctct attattaaag
     4741 agcccatttt gagtatgata aaattaccgt tacaggaaat gtgaggatag tatgatgaac
     4801 gaagtactaa aacaactgaa aaacagaaaa tctgtacggg tgtttgagga taagcccatt
     4861 gctgtggagc tgaaagaaga gattcttggt gctgcttttg aggcgccgac agcaggggcg
     4921 atgatgcttt atagtatcat tgatgttaca gatgatattt taaagcagga actggcaata
     4981 gtgtgtgatg atcagccctt tattgcgaaa gctcctttgg tgttagtgtt tgtggctgat
     5041 tatcaaagat ggtatgatgc ttatggtgta gcgggctgcg aagtgcgtca gcctggagaa
     5101 ggagatattc tgctggcttg tgccgatgct ctgattgcgg cacagaatac ggtggtcgcc
     5161 gctgaagctt taggtcttgg cagctgctat attggcgata ttatggagaa ttgtgagaag
     5221 gttagagaat tattagcact accggactat gtgctgccgg cggctatggt ggtttacggt
     5281 tatcccacag caaagcagct aaaaagagaa aaaccggcac gttttaatca acagtatcta
     5341 gtctttgaga atcagtatcg taaattaagt gccaaagaac atctggctat gcaggaagaa
     5401 aggcttaaaa agcggggatt aaacgatgtg gacggcaagg aagaaattag agctgtttgt
     5461 ctacgtaaat atctgtctga ctttaccacc gagatgaatc gttcggtggg agagtattta
     5521 aagaaattca gatagaaaaa aggttgtctg gcaagcaata actcgctaga caaccttttc
     5581 ttattgatca acgaaacaag cagtcgtagg ttactggctg cttgtttatg caagtgagat
     5641 aatttcgtta gcacaaaaaa cacggaacaa tgataaattg ttccgtgtct gatttatctc
     5701 tatttctcat ttgtaaagcg gttgagattg ttttatcatt catcatctag tcgctgttct
     5761 aagacatcct ttattttaag ttcgagctta tctaaatcta aatcggcttc ttgtttaata
     5821 ctgttgcgca gcatgggacc aacttgactg atatcatcta ataaatcata aagtagttca
     5881 agggcagcgg tggcgttgat gatttcaaga ggagtttcta cgatgttcgc aataattgct
     5941 tgttctgtcc tcgatgcaat gacggggttt ggcatctgtt caataaagac tttagtccgg
     6001 cgtccaggcc ctcggttctc ttcaataggt tcaagggcaa tgattttatc tgaacgtaca
     6061 aattttccgt aacccaatgg tataattagg tgttccttaa tttccattac cgacacatcc
     6121 tttaaagata tattattagt ctttcattta taggagatat cattcttcag attaatgatt
     6181 tgacgtggtt ttatgcaagc cgttgctgcc atggcaaatc catttctcta gacataacac
     6241 atgttttgaa tatatatcgc ttgcagttag cagggggtta atgtgactag taatgaaatc
     6301 gactgatgat ttttgccagg taaaatgata tgacttagtg cgtgactgtc gtcacttatc
     6361 atatgatttc ttccttcagt ataatgagtt taacctttat actattagaa tacgcagatg
     6421 tagttgataa ttattgggtg cttttgttat tatgtaactg tgacggggca aaagagatga
     6481 aggcataaag tggaggcacg ggtatgacgg gaggagtttg ggcttgatta gaaattaaaa
     6541 aaaggggagt ttacagtgaa ggatgtaatt acggttgatc agttaagtaa aagttacggt
     6601 aaccttaacg tcatagataa aatctcgttt tcagtcagtg agggtgaaat ttttgcgctc
     6661 ttaggcgcaa atggagcggg taaaactacg acactggaat gtatcgaggg aattcgcagc
     6721 tatgatgctg ggtcaatcac tatagatggg actattggtg ttcaacttca atcatcttcc
     6781 cttcctgcaa atattacagt tttggaagca tataagcttt tttgtaaatg gaatcatgta
     6841 cgcgaagacc cagagctgct agatactttt ggtttagctc tgttaaagaa taagcagtac
     6901 gaagccatgt ccactggtca aaagagaaga cttcatttgg tgcttgcttt aattaacaac
     6961 cctgctatta tttttcttga tgaaccaacg gcgggattag atgtagaagg aagagttgcc
     7021 ctccatactg aaatcaggaa acttaaagag cagggcaaaa ccatcattat ggcaagtcac
     7081 gatatggcag aggtagaaag cttatgcgat agaatcgcta ttttaaaggg cggcgatatc
     7141 gtattcatcg gctcatctac agaattaaca agcaggtacg taggtgaaag caaaattttc
     7201 ataaaaacac aattacccat tacgagagat gattttaaac atagcaccta tttagcaaca
     7261 catgaaggat atgcaagcgt acgtactgtt tccattggta atgctctgct ggaattactt
     7321 aatgaggcaa aagagaatgg taatgaggtt ctggatatca agatcgaacg agcatcgctt
     7381 gagcaacgat ttgtagaaat agcaacggag ggcaagtaat tatgagcgca tttttatatg
     7441 gtgtaattct gcagtggaaa ctagatctac gaaacaaggg cgtaatactg acatattacg
     7501 tagtaccact tctgtttttc gcctttatgg gagccatttt tacatccatt aacccaactg
     7561 ctaaagatag tttaattcaa tccatgacta ttttcggcac aaccatgggt gcgtttctag
     7621 gaacgcccac acctctagtg gagttatatg gcagtgatat tgtcaaggcg tataaagtgg
     7681 gaggcatccc tgtttggatg gcagcagtga ataattttat ctccgcattt tttcaccttt
     7741 tcatcatgag catcatcatc tatttgcttg cacccatggc ctttgcagcc aaagtacctc
     7801 agagtacttt atatttcttc ttatctctcg ccttctttat tgtagtttgt ttagccattg
     7861 gctctgtgtt gggtttattt attaaagata tctctaaact gacgatgttt tcacagctaa
     7921 tattcttgcc ttcattaatg ttatcgggaa taatgtttcc ggtggatcta ctgcccatag
     7981 ttctgcaaaa tgtaggaaaa gtatttcccg cgacctgggc attgatgatt ctgggggcga
     8041 atgaattcga ctttactttg tttttgccct tactttttat cctttgtctc gcagtttttg
     8101 ccagtgttta taaattatca cgagttggta tagaataaca ggtattttta tagaaaaacc
     8161 taccatgtcg gagtttagat ttgcgatgca ttcacggaaa aggtaggcat acaaagtttc
     8221 attaccgtag gatgtaatgt cgctacgttc tccattaata cctggaaaga acggatggcc
     8281 agaggccacc cctttgtgga aacgtaaatg gcgggagtga taagctgtac tacaaagggg
     8341 cgctatttta tgcctgggga cgacgaggcc tatcgtggca ggattactgc tgaataattt
     8401 ctctggcgta aaggagaata agtttgctta agagaaataa ttaacaatta tcgaactaat
     8461 tttgccgaat tatgtggtag gcagttggaa ggggttaggg atgaaaaaag atgtgttgat
     8521 atgtgatgat cagcaggttt ttattgagga gttcaagcag cggcatggtc agtattatga
     8581 aatccgggaa gtaaatgatg tggggatgtt agttgataaa ctgcatgata tgaagcatct
     8641 gccagatttg ctgttactgg atttatatca tccccgggca caaggggagg attttgagca
     8701 gaagcggcaa gtggcagaac aggaactggc caatttagat gagcagatta aaattactaa
     8761 gacggcggta gaagcagcct ggaacccggt agggttggag atcttaaaag atattcggca
     8821 ggactttagt gctgaggcgc tgccggtgat tatttattcg cagcgggggc tctttctctt
     8881 agatgatgaa aaggtacgtt tagtcgatga atatgatgcg cactggttat taaagcgaca
     8941 gttcgggccg attacggaaa agacgcgtat ggatagagta atgcgccacg aacagaagac
     9001 gacctcactg ccgaaaattt ataaccgggc gctaaccatc agttggagta ttacggctat
     9061 tgtcctggct gtttatgtgc tgccgattga ctctttgctg tggcagattg ccgtgggggt
     9121 actaggtggc ttggcaacca atgttttggc taattattat aataagctga tgaagccctg
     9181 gcaaaacaaa aacagccatc gttaagatag gaagtgcgag gtacaaaatg aaaaaacgga
     9241 tactaatatg tgatgaccaa gcaagattta tcactgaatt taaaaaaagg tttagtcagg
     9301 attatgagat agaagttgtg gatgatgtac ggcaagtgat ggcagcgctg cagagcggtt
     9361 cgcagctgcc ggatctgctg atgttagatc tttatcatcc gttagtcgta gacgcagagt
     9421 ttgaggagca ccgtcaggcg gcggaaagag aattggcccg tttagcagaa caggtgaaaa
     9481 aagcaaagac tgctgtggag ttaacctggt cgccgctggg agcgcaagtc ctgcaggaac
     9541 tgcgggagca ttacggggca gaggaactgc cggtgatgat ctattcgcag tcgggactat
     9601 ttgtactaga tgaggaaaaa gtgcgtgcga tcgaggaaaa cgacggcgag tggctgttaa
     9661 agaaacagtt tggaccgttg acggaaaaga cacgcatgga gcgggtgatg cgcacggctc
     9721 gccgtggtag gctagtgaaa aaatatcagc tgttggtagg tgtgagttgg gcagtaactt
     9781 tgtttatggt ttctttacac tttttcccct ttactgctgt agtctggcat ttgttggtag
     9841 gcgtaggcgg cggactgctc agctatattt ggattggtcg atttcagaga gtataagtaa
     9901 ctaaaatagt aatacagtct caaaaattga aaaaaaggta atgaattgta ataatatgtt
     9961 gcatattacc aaaattcatg ataagttttg tataggttca ctcgataata gcaaacctgt
    10021 tgaaagatag ggacgcaaag ctatagggtc taaagcataa gttatgtgct atgacagcct
    10081 ggccgccgaa cgtgatattt ttttaccttc acctgagcag taattactgg catcctaggc
    10141 gaacacttct taggcaaaat agatgtggaa gcagatctgc catatgaaat atcaaaaaat
    10201 tttgaaaaga gtattcgcag agtatttgat acgaaagaag aacaaatcgt tgaattcgct
    10261 taccaaattg ctgagggtaa aacactacac tattatacac gttgggttcc tgaatttaat
    10321 gacgaaggct tggtaaaatc agttctatgt atcgctagag atatcactaa aagaaaagaa
    10381 acagaactga gactattagc tggtgatgtg acggaacgca aaactataga agaagaaaac
    10441 tatcgcatgc gggaaaccct agagaatgtt gtctctagtg gagctgttgg tattcgtatt
    10501 gtagatagga attattcagt aatctggtca aatgaaagta gcgaagtagt ttattttagt
    10561 atcaatgatg atcaaccttg tgagattttt tcctatgcgg gtggaacctg tttatcccac
    10621 ctagattgcg atttagcagg tcagctgggg aaaattttta gcgaaaaaaa agaaatagtt
    10681 aagtgggagc agcaggttat tcgaaacggc aagctggttt ggttgagagt catagcgacg
    10741 ccaatccata ataaattgct ggaagttgaa agtgttgctt tatttttaat accaattacg
    10801 gaattaaaat ctgctttggt caaactctag gagagcgaag agcgctggca atttgccctg
    10861 gaaggaaacg gtgatggcct ttgggatcat aacctgcaag agaaaactgt atattattcc
    10921 cgccagttaa aagcaatatt tggttatgcg gctgatgaac ctcttgagaa tatctccgtt
    10981 tggacagacc gactgcaccc agcggataaa gagagtgctg tggctgaata taaggcacat
    11041 ttaaatggcg aagtagcttc cttttcctgt gagagccgat tgctttgtaa cgatgataac
    11101 tataaatggt ttcatatcag gggtaaggtg gtcagcttta cggaacaagg tgagccctta
    11161 cgcctggtgg gaacgtatac cgacatttca aagcgcaaag aaagtgaaaa gagaatactt
    11221 aaccttagcc agcgggataa tttgacggga atctttaacc gtaattattt tgaagagcaa
    11281 ttaaagaagc aggatattgc caataatctg cccttgggcg tgctaatgat tgatgttaat
    11341 ggtcttaaat tagttaacga tacttttggc tttgaaatgg gcgataaata cttacagcgg
    11401 gttgccgtct tactaaaatc tgtggtgagc aaaaaagacc ttattgcgag aactggtggc
    11461 gatgagttta tcattatcct gccacagaat aacttgcagc aattaaatca gctagccatg
    11521 aaaattaaac gtttatctga aaataaagat attgaacctt ttcctttaag cttagctctt
    11581 ggggcagcgg taaaagagaa tgaaaagcaa gatattcgta atgttactcg agaagctgaa
    11641 gatagaatgt atcaaaataa actatttgaa ggcaaaagct caagaagtac tgtagtgatg
    11701 tctttacggc aatctctgga tgaaaagact catgaaacgc aaaagcacag catgagactg
    11761 gcgaaacttg tcctgcgtat tggtgtaata cttgacctct cggacaatca aattgatgaa
    11821 ttaaggctgt tagcatttct ccatgatata gggaaaatag ctgttgccaa cagtattttg
    11881 gagaagtcaa atactcttac gctggctgaa tggattgaaa tgaaaaagca ctgtgagata
    11941 ggttatcgta ttgcaatctc tgtgcctgag ctgtcactaa ctggcaatta tatcctaagt
    12001 caccatgagc gatgggatgg taatgggtac ccgcggggac taaagggcga aaaaataccg
    12061 ctgctggcaa gaattattgc tgttttggat gcttatgaca ccatgactaa cgggagagtt
    12121 tataaaaaca agatctccaa agaagaagca ctagaagaga tagtccgctg ttccggaacg
    12181 cagtttgacc cccaagtggt taaggtgttc caaaaaatga tgaaaccagc tattttctct
    12241 aatcagttgg acctagagcg aaatcgctta catcaaatcg ctgaagaaaa ggggataacc
    12301 agtattgagg caatagaaca aagtacaaaa gtggaccaca ttatcaacca aataaaccaa
    12361 ctcgaaacaa ttagaaaaat gagcaaatga agtcgggtct catgatttaa tataaaaata
    12421 taagatatat ctagttggct cggctacacg acataatcca gctaaggcca tattcaagta
    12481 accaagtatg agttggataa agcagaggaa gtaaggtctt agaaatggtt attagcaaaa
    12541 cactctaaac agggtgtttt tttcatcgga aagtcaatta tctcaaccta gaattgcttt
    12601 ctttcctatc cgacgggggt tttctcgatg gcaggacatt tcccgttttc gctataatca
    12661 gagtataatt cagcggaaat gagggaaaac catggaacta ttgcaagtac agaatgtgaa
    12721 aaaagtttat aaaacgcgat taggggccca aaaatttacg gccttacaga atgtcagcct
    12781 ggaagtggct gaaggagaat ttgtagcgat tatgggggcc tccggttcgg ggaaaaccac
    12841 tttgctaaac attattgctt ctttagataa cctacggcgg gcgatgtatt ttcagcaggt
    12901 taccggccgg gctgagactc tgctggctaa ggaaatcttg ctgttaacga acgagcagct
    12961 agcagaagta ccagcccatg taaaaaaggt gattgccgat accaagttta ccacaggtaa
    13021 aaacggtaca ccgcaccggg cgatttttct agtagctgct gacagagcgc aggctgaggc
    13081 atggtaccag aaactggccc ccgagggaat aggtattcgt attaaaggta atctggtttt
    13141 aaatatagcg ggtgaaggag aacagccgct gacatttagc aacgaactgc gcagtcaggc
    13201 ttcaaccata gaaaatgtga tttggacagc ggatatctat agcgaaagag tggaaggtta
    13261 cggtattttt ggcaggtgcc atgctgcata ttttagccgc cagtaagatg attattaaga
    13321 tgctggaagt cttcgcttta tacaatgtta cgctgacagt gatgtgtatt actgccactt
    13381 gcatcatctt taccctaatt tacgtgctag tttaccggat gacagcacga acttattata
    13441 aactggtcaa ataataagtc agctaaaaga ggtggagaag gtttgtgtgc cagctgagct
    13501 gttttaccct atacaaaaat gatagcaaag cgccacaaat tacatcatgc aatttgtggc
    13561 gcttttttaa aatgcattat tgggttgggt caaaccatac ttttttgctc agattttata
    13621 gcttattttt tacctttagc atagggatag agctatccag ataagtttta acttatcagc
    13681 catccataat cctgcctgca atccacaata tagtcaacat atactgtctg gtccttgatc
    13741 tgatatagta tcaaatacca tttctcaacg aacatcttat gatacttatt cagcggtaaa
    13801 aattctgcgt tgagaaatgg aaatcgctcc ggcatagtag acagggaacg gagggcagcc
    13861 agtaattcat tttttgtttt gcgggctgca gcagggcttt tttgtgcaag aaaccgaaca
    13921 tggtccgcca gcatctgacg cgaacggtcg gagataatta ccttatattt actttttttc
    13981 cccatgctcc acctcgtcga taatgctatc cagagagctg tccaagtcat ccggtgtcac
    14041 tccggagcgt ccggctagac ggtcttcttc aacggccagc agttcttcgc gcagcttgag
    14101 cattttctcg cggcgggtaa aagcatctat atccatgacc acgagatcgc cctcgccgtt
    14161 cttggtaaga tacacaggct ccccggagga tttgcacaaa gctgcaattt cgttgtagtt
    14221 ctgccggatg ctggcggatg gcttaatccg catggtatca actccttata gtaatattct
    14281 aataaaatta tacccttttc atgctatgaa atcaatcaac aattcggtta ctacggcaaa
    14341 aaatccaacg ctctgctaag aaagaggatt ctatttctcc aaatagaagt tatattttca
    14401 acagctctat ggcttaggtt tttttgtccc atgcgttgta tcactcgaca attagggggt
    14461 agtgtgatga aggaagtact aaaacaacta aaaaacagaa aatcagtacg ggtgtttgag
    14521 gataaaccca ttgctgcaga gatgaaagat gagattctgg gtgctgcttt tgaggcaccg
    14581 acagcagggg caatgatttt gctacagaga tgaaccgctc ggtgggagag tatttaaaga
    14641 agttcagata agccactgta gtcctaaaac ttaataaagg tatgaaccct aacattgcca
    14701 ctacattact gtagtggtct ttttttggtt tacaacaact tgtcttagat gtgtgataat
    14761 ccttgaaatt agaacatatg ttactagcga taatactaga tggggggaag ggagattaga
    14821 tgaagacaga agaattattg cagtatcggc gacagttgat tgaggataca gtgaggttaa
    14881 agaagaggga aagggtgcca cacttttcta atatttgttt gtggttggtg ctggattatg
    14941 gctgtaggct gacggaggcc tgcagtgact ggcagctgat ggaaaagtgt cagctggcct
    15001 tctttaacaa gtacaaattt gattttgccc aaactattgc cggcctgtta tgtcatccca
    15061 tggcaatgat caaagatatt ggacagggtt tctattttta taatgatgtt aactctactg
    15121 ttagtctcag agatatgcgt ttgctgctgc cgaatgaata tgagcagttt attgctgatt
    15181 tgccaagata cacctgggaa gttttgctgc cgcgtaaata tgagcgttgg ccagaactta
    15241 aagttaaaga cctgctggaa ccgcttgctg aatatcagag atacactgat tttgccacga
    15301 gaatgggtga agcagcagtc acaagatata gtattccccg ggctagtgca gaaatggtgg
    15361 ggatgcccgg tattgaaatg ctgtgtaatt tatataccgg tatgaagggg ctggcgatag
    15421 atatgcgcag gcagcctgaa aaagtcaaag ctgccgtgca gatgttggaa agcggtacag
    15481 acgcagtggt acagcgcatc ctggaggagc ccagacagaa taatgtggct gcttttgact
    15541 tatggctggg tatgctggca cataactttt tgagtattaa acaatgggat gcatactact
    15601 ggccgtggct gaaaaaagta cttgatgcgg tagtggcagc tgataagaca gcgtatatct
    15661 ttgttgaggg ctatgtgagc cgcttttatg attatttccg tgttttccct gcaggccaca
    15721 ttgcgctgca tattgagcaa gatgatgttt acgaggtcaa aaaaaatctg cccaactgct
    15781 gtgtggtagg cggcatgtcg tctacgctgc tgggcagcgg cagtaaggaa cagtgcattg
    15841 cacgtgctaa acagctcatt gatgagttag gcaaggatgg tgggtttata ctcagccagg
    15901 ataaaatgat ttcttatatg cgggacgctg atcccgccaa cttaaaagca gtctgtgatt
    15961 ttgtcagtga atactatatt taaacgggag gattacaatg gagaagaagg atattaatct
    16021 ggagttaagc ctcgctaact atgaagaagc agtagcagaa attagcaagt tgcttgattt
    16081 cctgccggca gataaagcag tacgaaaaga gtacgtggac gctattgcca acaaactgct
    16141 ttggctgtaa gtacagatat atggtaggtt gttgttagga ttatgtaaac gtagtattaa
    16201 aagagataga caacgtagca aatctattgg catgaatctt acaaaagaac agtattacca
    16261 aatgattggt tactaatcgt gtcaagttac atatattcgg aatgtcagcc gagcgcacca
    16321 agaccactat gcgtttgtct aataaatgca aaaaaaacaa gcagccgtag gttagaatct
    16381 taaccaaggg ctgcttgtct agatacttaa gctgatatta gctgatattt gattagactg
    16441 gtatgacaat atgcctacgg acggtgttct ctatttccac gaggagcggg agcaggtata
    16501 cttgctcatc ttcgttcaac ttgtactttt ctattaattc ttcctcgctg aggattattt
    16561 ttcttttgga cattatttat cccccattcc tgttaaatta atatacgcca tactctacgg
    16621 cagtatccac catcgcctga atgttttcta ttttagcatc acccgggttc agtacgggct
    16681 tattcatggc aaaaatgtag ttaccattta cagctacaat atctagtagg cgttttactt
    16741 cgtcactaac ttgctctggt gtggcggtgc gcagcatatg agtcttaaag aaggtgctga
    16801 tgatatggcg tttgtcaagt ttctccacta ctaatttggg atcagccatt tcaaagccga
    16861 tttccactct gcctggcaaa tcattaagag cttcaagatg tggagtccaa tcgtgttcac
    16921 aataaatact ggcatgcagg ccacgctcct ggatagcctc aattaacttt ttaaaggtgg
    16981 ggaaatagaa cttttcaaag ttcttggcat tgaggaaggg agccatatgt aaggggagcg
    17041 taactttgtt tattttacca ggaacttttg tgactctgtc tagggagtca caggaaaatt
    17101 gcagcatcgc ctcggcggca tcgcataccc attctggcgc tctgcgcata tcaatgcaga
    17161 tgtcagagaa gtttcttaag aagtcagcaa tataatctaa aggctcgcaa gacatcatcg
    17221 ccatcgcctc tatattgctg cgttcatatt tttctactag ctgtggtgtg cccatgcccc
    17281 agcttttaga ggctacgaca ttgcgggcga tattaagtct taattcaccg tagcgctcat
    17341 cttcttccag aatgccaaat attcttggcc gtaccacatt agcccaatat tcaaaggggt
    17401 ctttaataaa gtcgggatat tcttcaaact tcattggtga gtagttaggg tgttgataaa
    17461 agccatctct gccctgcacc ataaacttac tcagggaata gcgaaaggca gctgccgagt
    17521 tttcagggtg ggttggtaaa gtatcactgt tgattagttt ggccatttct tctgcgactt
    17581 caaaacaatg tttggcgctg tagtattcgc gaaatagtga ataaccttga tgctgtatcg
    17641 catattccag actgatcatt gctgttacgg ggactctttt gggttttctt ccttcccaaa
    17701 tatccgcaaa taactgattt ctctctgctt gtagactact cattttgctc tcctccttta
    17761 ccttcagaac gaattatacc gtttgttaac tgcttgcagc agtattctag ctaaaaaaca
    17821 accctccttt ctagctaaac atttattaac ttttacatac tgatgccgga atcctgccta
    17881 ttttttgaat tgtgcaaata ttaatactat caattattat tatgccctgc aggtaactac
    17941 ctttgttata aaaaaatgcc cccaggttag gtgttgtcta acctggaggc actcccggtt
    18001 gctatttatt cgtcaaaagc ctgactaact ttccagacct tccacacatt gcctacgaga
    18061 tctgggtttg gctttaatgt atctttgcca ggctgccaac ctgagggggt ggcttcagtg
    18121 cctttggagg cgcgtactag ttgaaaggct ttaatttgcc ggagggactc agctacatta
    18181 cgtcctacag aaggtgtgag aacctcgaag ccttggataa tgccatcggg atcaatgata
    18241 aagcgtcctc tggtttctac gccagcatct tcattataga tgccgtacat tttgccgatg
    18301 ctgccatcct ggtcagaaag catggggaag ggaatgtctt tattaaccat cttcgatagt
    18361 tcatgttcat tccacatttt atgtacatag atgctgtcta cgctaacaga aagaacttct
    18421 acatcgagag cttttaactc ttcatgcttt tcggcaactg ccgaaatctc ggttgctcag
    18481 acgaaagtga aatctcctgg gtagaagcag agcagaaccc acttcccttt aaactcactc
    18541 aaatcaaaat tcataaattt cccctgaaaa aacccgggtg ctgcgaaatc tggggcaggc
    18601 ttacctactt tgggtaacat agtatgttcc tcctttatgg gggctattgc agcattagcg
    18661 gctggcgggg cagctttttt aattgctggg cgctgacacc ctggtttaaa ttcttctggc
    18721 atatgtatcc tcctcgtata attagtttag agctcatttt tgagtcagtt tccagttgaa
    18781 ccggacgact agaaacgact taaatgaatt tccagtttaa aatgacatgg tgatagtgta
    18841 gacaatcccg catcttagcc cgaggggcgt ggtgaaacgg aagccggggt agcgctgctc
    18901 cgtaccgaac cagtaggcgg tattgaaagg tcttaacacc atcggtcatg tcttgagaaa
    18961 gcctaggctt cctcatcctg ctttaccatc accccgtttt acccttatta ccggatgggg
    19021 aagccgggat tactcaacag aataactact acagtcttta cacattcccg gtgcgcgttt
    19081 gcgtgtggac cggtaagtgg gatgcactca ttccattcgg attgggaacc gccgtttgga
    19141 gcggacactg ttggagagaa gtacaactag cggcaggaat caagggcgag ctttagcgag
    19201 ttcatcttga cccttgattc cctcaccgta caaatatcct attatttgcc ggtctgcata
    19261 caggccggct gccttccggc gaggacgggg tttggggcag ccccccaagc ggatcctctt
    19321 tcctgtaaga actttgctct ttgacaattc catttgtttt tttaatgttc aacaagaatg
    19381 ctcatagggt atttacactc agacctgcct gtggaacaat ggggtcgaca agcttttttc
    19441 aggagatttc ttttcctcct tgcggaccga aaggtactgc ttattaagcc tgttacccgt
    19501 ggcccatcac tcatgttcta accgctggtt gcgcctccat accacttggg gagtagcacc
    19561 cttaattgtc gggtggtatg gatgagtgct gatgacgagg ctgaagtcgg tggtttccac
    19621 gggcacccag gtctgatatt agcttgatta ttttgctaag aggaggtgat tctatgtcac
    19681 caatgctgat tggtgtagac gtaagcttac gctcccacca tgtacagttc atggatgggg
    19741 ccggaagatc gctcgcttct ttttccgtgc ctaacgatca atccggggca gataccctga
    19801 ttcataagat gttagacacg gcgagtaaag aaaagctgaa gcatcttcac atcgggatgg
    19861 aggccacgtc caaccttgga tggcaccttg cccattatct gaaaagcgaa cttcaaaact
    19921 atgagccaaa cgtaatgtct caggtttatg tcttgaatgc aaggaaggtg gctcgtttta
    19981 agaaggggta tgacaccctt cccaagaacg accgcatcga tgcctgggta attgcagacc
    20041 atctgcgatt tggtcgactg ccccatgaga tgaaagatat catccaatac gaagccctgc
    20101 agcgccttac ccggacccgc tttcatttaa tgaagaatat taaacgggac aagacctatt
    20161 ttctaaatca ggtctttttg aagttcagcg ggatgcgtca ggacaacccg ttctcaaata
    20221 cgtttggcag caccagcctg gcggtaattc aggaactgga gccagaccag atcatggcaa
    20281 tgtccatgga agaactcatc gaattcctac aagacaaagg caaaaaccgc tttgaaaacc
    20341 cggaggagat tgcgacttat ttgaaaaaga ttgcccgttc atcctaccgg ctggataaag
    20401 ccatggccga tccggtaaac atttctttgg caacaacact aagtgtaatt caacatttgg
    20461 agtctgaagt caagaagctg gataaagaga ttgccaagat catgaaaggc attcctcaaa
    20521 cactgacttc cgtaaaagga attggccctg tctacgcggc cggcatcatg gcggaggtcg
    20581 gaggcataga ccgctttacg aatcatgccg acctagccaa gtatgccggt cttgtttgga
    20641 accagaatca atccggcgaa tttgaggctg aagaaacgtc acggatgcgt accggcaaca
    20701 aatatcttag gtattatctt gttcaggcag cggattctgt ccgcaaacat gataccgagt
    20761 atgcagcttt ttatcagaaa aagtatgacg aagtcccgaa acataagcac aaaagagccc
    20821 tcgtcctttc ggcacgaaaa ttggtacggc tggttttcat gctactgaag accaatacac
    20881 tgtacacacc acctgagagg aggaatccat agccttaccg gattcttaca ttttttacag
    20941 tttgcccacg ctattggatg tcgaaaaaaa tgacctatta ggtgggtttt gtttaatgat
    21001 cccgttttta cctcaaagac acggttttga ctgatatttt tccaattctc gaaaattagc
    21061 ctcttgacat atcaccgctg tgcttcacaa aatgctagat gttactgcat gttaattata
    21121 tccaatacag caataaatat attaataaat tactaaaagg gacagggttt tgggggatta
    21181 agtaaaccaa ttcattcgcg tggagagtgc ggtgtggttt gggaagctag atgcagaaaa
    21241 agctgctaag atgcacatga taagaggaag aggagaataa ggtaagatgt taatgttaag
    21301 ttggggagga gatggcatga aacgttttct gttagttgaa gatgaacaga ttatggccaa
    21361 gaatattgcc tttttttgga gcgggaaggg tatcgggttg atattgctta tgatggtgaa
    21421 gcagcgctgg agttgtttgc caaccagaaa tatgatttag tgctgctgga ttggacctta
    21481 cctaaattgg atgggctgga agtatgtaaa cagatccgcc aagcatcccg ggtgccgatt
    21541 attatgctga cggcgaaagg tgaaatctta gataaggtga ttggcttgga agtgggtgca
    21601 gatgattatc tggtcaagcc ctttgaccag cgggaattgc tggcgcgggt tcatgtgctg
    21661 ctgcgtcgct tgcctgcagg gctggcagct gattcctggc tgcaatatga ggagctgcgt
    21721 ttagatcggg agaaattgct gctggtctgc ggtgaggaag ctgctgcttt gaccgctaat
    21781 gagtataaat tattggagct gatgatgaaa aagcctcaca atgtctttgc gcgtacatat
    21841 ctttatgaag aagtttgggg tgaaatgccg ggttacaatg accgcactgt ggatgtgacc
    21901 atcagccgct tacggaaaaa actgctggag ttgtccggga aaaaatactt tcaggctgta
    21961 aggggtctgg gttaccggtt aggggaacaa ccatgaaaat ccaatctcaa ttgtggctga
    22021 ttctctctgt gctctttctg gtaatgtcgc tagtcgtgta cttggtcgtc gcagacgctt
    22081 atgaggcacg tttacagaca agctatcagc aaatctcttt tgcgcaaggc tcggcgctgc
    22141 ttgacgaatt aacgaaagca taccctgact cccggcagcg cagtagtggt tatctgcaaa
    22201 actacagtga acagctgcag gcccggttaa tcctgctgac tgagcaaaaa aaggtgttcg
    22261 ccgacggctt tgcggtactg gaagcaaata gcgcactgga tttgacggtg ttaaaagcag
    22321 gtacagggta tgcttctcac tttgcgcaga cggaggcttt cggctatgtg cagtatacgc
    22381 tgctgccctt tgacaaggct tccagtaaag ggtatttact aatcattcag gaagctgggg
    22441 ggctatctgc tgaattaagc tcctttcgtg cttggatgct gcggctgctt gtgctggcac
    22501 tgcttagttt ttttgtgctc tcctatgtgg tagcagcttg gctgacgaaa ccgatgcgca
    22561 gaattattgc ctcactcaag caaatcacac cgcacaaacg tgactttgca ctcacctacc
    22621 ggcggcagga tgaaattaga gagctgattg cagccattgg cagaatggtg gaagaattaa
    22681 atctctatga tgagcggcaa cggcgttttg tcagtacttc ctcccatgaa ttaaaaacgc
    22741 cactggcgac gatacagctg attttagaga acctgcccta tgtgcgggaa aatgaagaaa
    22801 cttatcagga gtttactgag gatctggttt ttcaggttaa gaaaatgagg cagatagtgg
    22861 agcagctgct gcagatcatg cgcctggggg acagcccgct gcaaaaagag acggttagtg
    22921 aagaggatat ccggcagcat ctgttgcagg tgtttcaata tctggtacag gaaaaagagc
    22981 ttacctggga gtttgagctg cagaaaacta gctttaatgt tgatcgtctg ctcttcttgc
    23041 gggcgttgga caacttggtg gctaatgcta ttcggtattc accccagggg aagacaatta
    23101 aaatagtggt caagggtgat aaaatagcaa gtgtggttag tgtttgtgac caggggatcg
    23161 gtattgccgc agccgatcta ccgcagattt ttgaaccctt ttatcgtgct aatgatgcta
    23221 cggcctataa tcaggaaggc agcggtttag gcttagcgat tgttaaacag attgccgagc
    23281 tccatcaagg aaagattact gttaattccg tgccccagga gggttcatgc tttcattttt
    23341 tgctccctac ttaggtaaca aaattgttac atttcatcaa tgatcatgat acaaaaaagc
    23401 agtatattaa aggtacacaa aaaaaggtga catagaggag gtttaaccat gaagaaatta
    23461 ctaaccagta tcggaattat cttatttagt ctgctgcttg tggttggctg tgccaaaaac
    23521 aacgccacac caccgaaaaa cgatgagcag gagccgccag cagtagtaga tcctgatgac
    23581 aaccaggagg gtcccattgt gatagatcca gccgaaccaa cagaggagcc tcttgatgaa
    23641 aatgatttga cacctacccc ggaggataag gagcaagtgc atcagatcgt gctttacttc
    23701 ccggatagcg acctgctgcg tacttatcgt gtcaaaacac aaattacgct aaaagagcat
    23761 gaacagctgc ctaaagcagc gctggaagct tggctggccg gtccccgaga taagaaacta
    23821 acgggtctcg ctgatgaagc ggtagtgatt gaatatgtga aagatgtgaa gggcgttgcc
    23881 caagtgagct ttagtaaaga agttcaagat ctcaacgtcg gtttcgaggg cgaattgctg
    23941 tttgctgaac aggtggccat gattatgcag caatttggct atgagcagac gcagctgctg
    24001 ctggaaggca aagttgtaga gaccctgttc ggacatcttt atactaaaga gccgatgatg
    24061 gccaaggccc cagcagacta ccgttgggtt gaggaaatgg atactcgtaa atttgttctg
    24121 caaaatactg cttttcgtat ttatgagccg gcaccaggca cggtggtcaa agatcggatt
    24181 gtgatccgcg gcctggcacg cgtttttgag gccaattttc tgtatgaatt tgaggatggc
    24241 cacaatattt tagctaaagg ctatactact gcctcagaag gagcacctgg ctggggggaa
    24301 tttactatcg tcatagaaat tgacgaagtt gccaataact ctggtaccat catcatatat
    24361 gaggcgagtg ccaaagatgg ttcgagaatg aatgaactgc gcataccggt gactgttgct
    24421 aaatagtaat gagcggaaga gaacggccat ttaccattac ggtaagtggc cgttcttagt
    24481 ttgataagtc tgtaattgtg gcggaatcaa cttgcttaaa ctaatcagat aatttataat
    24541 attattgtta gtataaatat agagacaagc ttccggttag ttcgtgggag aagattattg
    24601 aaaggagatt aggttatgaa agaagatgta aaaaaattac aacaagaacg tggtaaactg
    24661 tttagcgatt tttacaacaa caagattccc tcaagaatgt ttgttagtgt ctctgttggc
    24721 agccatattt tggcagagtt tggtggtctc aatgacttcg aagtacagta tgactactct
    24781 ctattagcag agccggcaaa gaaaatctgt gaaatgattt actcagatac cagcccggta
    24841 tcgccagtgg ggataatctc ccggccgcct tctttttatc agttattagg gtctcagtcc
    24901 tttgtcatgg ggaacagagg ctttgtgcag catcctgaag ttgtgggtat gcatgaggat
    24961 gaatatccgg aactaattgc tgatccttat tctttcttgt tagaaaaggt aattcccaga
    25021 cagtataaag aacttgatgc ttctaacccg gtaagaatgc ttaaccggat gcagatggct
    25081 aagtctgctt tagctcacga ttctgcctct gctatgcctt ggtttttacc attagctaaa
    25141 gagtatggtt attatcaagg tgcacctcgt ggttctggtg gtttcacgga agcaccatat
    25201 gattttattg ctgaccaact gcgcagcttt agtggtatca gtaaagatat cagaagacat
    25261 cgcgaactgc tgaaagaagc ttgtgatgca gttttaccgt taatgtttag aatgggctta
    25321 ccagctaatc cacatccgga aggcgccgta gggactccct tacatatgcc gacctttatg
    25381 agagaaaagg atttcatgga agtctggctg ccttcgtata agaagatgta tgaaaactat
    25441 gcagctcttg gcatccgtgg ttcagccttc tgcgaagatg actggatgcg ttatttagac
    25501 aatctgcttg aactgcctgc tggcatgcgt ttagcctttg aatatggtga tccgcagcaa
    25561 attaaggaca agcttggcaa gaagtttatt ctctccggct tgtatcccct tgatttaatt
    25621 aagcacggta ctaaagaaga gtgtgtctac aaagtaaaag aactgcttga catcatgatg
    25681 cctggtggcg gttacgtctt tggttttgat aaaggaccgc taaccttatc ggatgttaat
    25741 ctagagaatc tagctgctgt ggccgaaact gtcagagact atggcgtcta tgataatgca
    25801 ggggaaagct ttggtactcc tgtcaatagt gaagggtttg tctttgatga aagtatcatg
    25861 aagcttgatt cgaaatactt ctttaactgg gatgagttta aggcaaaata tccgttaact
    25921 cccgattcag cacgggcgaa ctttgagaag tatgataaag agctatttga tcagtacatg
    25981 ctgatgttaa tctaataaga gaagtaaaga aaaagtatag gcgcgtagag tgcgcctata
    26041 ctttttcttt gggaaatatc acgtaaaatg ttgtgccttg ggggctggta tctacttcta
    26101 tcttggcctg gtgccgggac cattatcttg aatggttagt gttactgtgt cagtagtttt
    26161 atctggattc ctttcatcat tcttgtttaa attaataatt ttctgcaaat attttcaaat
    26221 tcctgagcag gccgttattc gccaaggttt agtcgacaaa ctattggttt aagtcagata
    26281 atttataata ttgtcatttg tgtattgact taaggttaat attgacaata taatctaagg
    26341 tggacaaggt ctaagactag gtcagcagta agggaaatcg aaaggagcta aaaaaatgaa
    26401 agaagaagtg aaaaaattac aacaagaacg cgccaaactt ttcagtgact tttataacaa
    26461 caagataccg acaagacaaa ctgtgagtat gtccgtaggt aactttatcc tcgcggaatt
    26521 cggcggtctg aatgtctttg aaattcaata tgattactct aaactagcag agccagccag
    26581 gaaaatttgt gaaatgattt actcagatac tagtccggtt atgcctgcta gtatcatctc
    26641 gcggacacca agtttttatc agttgttagg ttcccagtct tttgtgatgg gtaacagagg
    26701 attcgtgcag catccagaag ttgtgggtat gtatgaagat gaatatccgg aactgctcga
    26761 agatccttat gcctttcttt tagaaaaggt catcccccgg cagtataaag aacttgatgc
    26821 cactaacccg gcaagaatgc ttaaccggat gcagatggct aaatcttctc tgacccacga
    26881 tgctaattct tgtatgtcgt ggttcctgcc gctgcaggag gagtatggct accatcgcgg
    26941 tgcaccgatg ggttctggcg gcttttgtga agccccctat gatttcatcg ctgaccaact
    27001 gcgcagcttc agtggtatca gtaaggatat tagaagaaac cgcgaagtat taaaagaagc
    27061 atgtgatgca gtcctgccct taatgtttag aatgggctta ccggctaacc cacatcccga
    27121 aggcggtgta ggaacaccct tgcacatgcc gacctttatg agagagaaag acttcatgga
    27181 agtttggatg ccctcttata agaagatgtt ggaaaacttt gctgcgcttg gtgtgcgtgt
    27241 taatgccttc tgcgaagatg attggatgcg ttatttagat aacctgcttg aactgcctgc
    27301 cggcaccaag ttaatgttcg aatacggtga tcctcaaaaa attaaagata agcttggcaa
    27361 gaagtttatg ctgcaaggtc tctatcccat tgacttaatt aagcacggta ctaaagaaga
    27421 gtgtgtttat aaggcaaaag aactgttgga tatcatgatg cctggcggcg gctacatctt
    27481 tggttttgat aagggggcac taaccttctc agatctcaac ctcgaaaacc tagcggctgt
    27541 agcggaaacg gtcagagatt atggcgtcta tgacaatcca ggtgaaagct ttggcacacc
    27601 acttaacagt gaaggctttg tctttgatga aagcatcaag aagcttgatt caaaatactt
    27661 atttaactgg gatgagttta aagccaagta tcccttaact cccgaatcag cacgtgctaa
    27721 cttcgagaag tatgataaag agttgtttgc ccagtatatg ctgatgttaa tttaaaaagt
    27781 aaaaacaatg aaacaagagt gttgcgagct acttttaagt agtttcgcaa cactctttgc
    27841 tatacctaaa cgccgacgag ctgcctttgt ttttgtttag tgcgggcaat tttaacattg
    27901 tcttgtctgc ggtcccgcag ggcttctact gcattgggtt tatcctcata gcggtagtcc
    27961 tggcctttct tctgctgatg ttgagcaatc actgcgtggc taggcacagc ctttagatca
    28021 tgattaactg tttgttgata ccagaagaag cggtgattat ctggcaggtc attaacattt
    28081 ttgagaactt cctggtcagc agtgagttct acctgttgac cgaggatttt tcgtacatag
    28141 tccttagtgt cgtggaactg cagcagttta ggaaaaactc cgccggggat cacctgctgc
    28201 caatctttat gttcgtgttg ggcgagcaga tctttggcgc gatgcagctg agcaatttcc
    28261 tgccgcaggt gcatctccca aatatcttta acatggggat ctctttcatc ttgatagaaa
    28321 gagtagtaaa gatagcactc catgtattcg tgcatcagta agttttccag ccaagtgcag
    28381 tttggatcca ttaatgctcc gtattggctg acatgctctt cttcgattaa ggcaatttcc
    28441 tgatacagtt ggcggcccag atcattatag taggtaggac cgaggttcat ataaaagttc
    28501 atggtttgct gttcagcggc ggtgataatc ataatgttta acttggtacg aatgtcggcg
    28561 cttttaaaat taatgtagcc tcttactgta tcactagggt gacggtgttc ggcgatggtc
    28621 ggtcgacctg gcgtaaattc tacatattct ttaactaatt tctgtgcagg aatattctta
    28681 tccagctcca ggaggttagc gtagcgatat agatggtcaa aatc
//
LOCUS       DASRMS010000005         5341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_403272, whole genome shotgun sequence.
ACCESSION   DASRMS010000005 DASRMS010000000
VERSION     DASRMS010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403272"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(524..829)
                     /locus_tag="VFC74_00235"
     CDS             complement(524..829)
                     /locus_tag="VFC74_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23801.1"
                     /translation="MPKQDAEWGGKREGAGRPSTGRKARSVYVTDKEHEAIKDLIKKM
                     REGEEMTKYRGIVKDDMGNMESRQTRWYSTYKEAHDAAEKLCERTIGERGSVEVEQG"
     gene            complement(831..1058)
                     /locus_tag="VFC74_00240"
     CDS             complement(831..1058)
                     /locus_tag="VFC74_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23802.1"
                     /translation="MKRYAAYHIDGSKANEFREAAVKLFSNEELKREIQERMMNLNRE
                     TMSFDLRVGPGCPKEIEELAKRFGKGYNSYA"
     gene            complement(1220..2293)
                     /locus_tag="VFC74_00245"
     CDS             complement(1220..2293)
                     /locus_tag="VFC74_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZK23803.1"
                     /translation="MRIVLNPGHGGKYRSNVGPTGYIEADGVLDIGLRLKALLQAHSV
                     ATVYMTRETDKTLELYDRAELANKWDGDLYISLHTNAGGKTAEGTEIYHTKRNEWGTK
                     DAAEAKRVATIVQKNVVSNTGMVDRGTKTKTVTNKTSPIYGMDYYADIRLANMPTLLM
                     ETGFHSNPEEEAKLKTPAFRQKIAEGILAGLLEAYPAIKKPVEKPEQSAVRLYRVQTG
                     AFKHRDNADRLADKLEKEGYDTYIVESDGLYKVQTGAFAKQENAIALAKKLKAADYDV
                     YITTKSGQPAAPPKPEPKPTIKVGSKVKIKAGAKDLNSKQTISSTARNRISIVSELKG
                     SRAVVTYGGVVVAAVNVKDLILQ"
     gene            complement(2296..2694)
                     /locus_tag="VFC74_00250"
     CDS             complement(2296..2694)
                     /locus_tag="VFC74_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZK23804.1"
                     /translation="MEKGLIILKAAVAVVLGALSTLLGGWDVALQVLVVLVVLDYISG
                     VVAAFHRKEVSSYKGFWGIVKKIMLFVPVAVATMLDIVIGQEILRNLAIWFYIANEGI
                     SILENLGKIGVLVPPGITDALEQLKSKGGK"
     gene            complement(2759..3511)
                     /locus_tag="VFC74_00255"
     CDS             complement(2759..3511)
                     /locus_tag="VFC74_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23805.1"
                     /translation="MAMLGDTIIIGGFLNTELIRAKSIVAEMLAVEDLAAITGKFSGD
                     VWVGGDIVVGGQGVLSVFQFESSGGGSWGDSGGWSSLGLMEWELTKGRMGVTVFIPDN
                     FTVTRATIYLYAMPMFYDNPAGLGSHPTKWKQSRNLRLYHSDGTEGYHYRTANSGDSQ
                     VLWRSGVNITSAVLGVSAWSPTLAYSGTDPNNTSNKIQIRSGDLTNYLSSGMRRTFYV
                     ETTDAGTEANYAVNEGLGKMIVLVEGYTSVSQ"
     gene            complement(3683..4210)
                     /locus_tag="VFC74_00260"
     CDS             complement(3683..4210)
                     /locus_tag="VFC74_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23806.1"
                     /translation="MALYKESMINKIEDDLAGRNQNWDALDTYLAEKAFKGALLRKGT
                     AQSIPHNTATALTWTDVVYNIGNIYSAGLPTRLTIPSGVNKIIVKASVGFQENTTGKR
                     EVYIYKNGNGNYNGRATATVIPVSGTGAIAHVVSPVLEVVAGDYFEIFAMQTSGGSLN
                     VTDWYNIWFAIEAIE"
     gene            complement(4210..>5341)
                     /locus_tag="VFC74_00265"
     CDS             complement(4210..>5341)
                     /locus_tag="VFC74_00265"
                     /inference="COORDINATES: protein motif:HMM:NF018329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZK23807.1"
                     /translation="YVDLLANRGGDYGKRFTYTKDLLSVTKTVHRDDIITALYGYGKG
                     EEIVDETGEPTGGYGRRIDFADINDGKAYVENNDAKAIWGRNNPDGTKSHVFDKVEFD
                     DCEDPHELLALTLAKLEEVSAPLITYQATVIDLKAFGIEHEGVELGDYVAVIDKEFKP
                     ELRLKARTIKIVRDLLEPENNDITLGNFMPSFVDKINEQEKYISNFRGRQGVWDRAKI
                     IDSDGINTQYLNGVIDILKHQLLSTQSGWHTDDNGNLIFENNDKTAAMMLTGEGFMLA
                     NAKLPDGEYDWRAFGTGAGFVADEIIAGILKGGKVRFDLTNGTFLIGNGVEDYQLLFD
                     GDTLDYKGKLHITPETTYEAEEIYTWQRYAGMTWQQVINEVM"
BASE COUNT         1240 a         1411 c          985 g         1705 t
ORIGIN      
        1 aacaaggcca caatcccgcc gataaccatg cctaaagtcc ctaaccatat agacaatatg
       61 agtggtacag ttataaccag agttaataaa cagccgcatc cttgcatttt ttctcccgct
      121 ctttgcattt ttttgcctgc attttctaaa tcagccataa actccccccc ttatttttac
      181 cttaccataa ttcggacgag cttacaagtt cagctttacc ggcggccttc cagatcgtat
      241 tctttaatgc actacataaa aatccccgct ttctaacagg tataatcacc ttaggcacat
      301 ttaaaaaagc tcgtacaggg caattgtggg cttcgttttt tcgcattttg ggcaaaaaaa
      361 taagcccctt gcttggggct aggatttgat atatctgtcg tattcttcgg ccgtcatcct
      421 atatttgatt agcacatctt cccctgtcct agtgctacat tcttcgcagt tttctttatt
      481 gactatagca tagcagcaat cggcgcattt tttcaatgtc aggctatcct tgttcaactt
      541 caacgcttcc cctttcgcct attgtccgtt cgcaaagttt ctctgctgca tcatgcgcct
      601 ctttatatgt actgtaccac cttgtttgtc tgctctccat attgcccata tcgtctttaa
      661 caatacctcg atattttgtc atttcttcac cttctctcat ttttttaatt aaatccttta
      721 ttgcttcgtg ctccttatct gtcacatata ccgaccgtgc tttccgtccc gtggaggggc
      781 ggccagctcc ttcacgttta ccgccccatt ctgcgtcttg tttaggcata ttaggcataa
      841 ctgttataac ctttgccgaa cctttttgca agctcttcaa tttcttttgg gcaccctggg
      901 ccaactctta agtcaaagct cattgtttcg cggttcaggt tcatcatgcg ctcctgtatt
      961 tctcttttta gctcctcgtt gctaaacaac ttaactgctg cttccctaaa ctcattggcc
     1021 ttgctaccgt cgatatggta cgctgcgtat cttttcattt ttttaatccc ctttccttat
     1081 cttgatttaa ttatagcatt atcaagcttg attgtcaaca tataaaatca agttttttaa
     1141 gtttttttca actttttttt taaaaatgca aaaaaaaata agcccccagc caaaaggcca
     1201 ggggcgctat atcgctatgc tattgtaata ttaaatcctt tacattgact gccgccacta
     1261 ctacaccgcc ataggtgact accgcccggc taccctttag ctctgacacg atgctaatac
     1321 gatttcttgc ggtggagctg atagtctgct tactatttaa gtctttagct ccggctttaa
     1381 tctttacctt gctaccgact ttgattgtag gcttcggctc cggcttcggt ggtgcagccg
     1441 gctgtcccga cttcgtggtt atatacacat catagtcggc cgccttaagt tttttagcca
     1501 gtgcaatggc attctcctgc ttggcaaaag cgccagtctg cactttgtaa agcccatctg
     1561 attctacgat atacgtgtcg tagccttctt tttcgagctt atccgcaagc ctgtctgcat
     1621 tgtcgcggtg cttaaatgct cccgtctgga cgcggtagag cctgactgct gattgttctg
     1681 gcttttctac aggcttctta atggcggggt atgcttctag cagtcccgca agtatgcctt
     1741 ccgctatctt ctgtctaaat gccggtgtct ttaactttgc ttcttcttcc gggttactgt
     1801 ggaatcctgt ttccatgagc aatgtcggca tgttggcaag ccgaatatct gcgtaataat
     1861 ccatgccata gattgggcta gtcttgtttg tgacagtttt ggtttttgtc cctctgtcta
     1921 ccataccagt attagatact acattctttt gcacgatagt agccactcgt ttagcttctg
     1981 ctgcatcctt ggtgccccac tcgttgcgtt tggtatgata gatttccgta ccttctgcgg
     2041 tcttaccacc tgcgttagta tgcaaagaaa tgtacagatc accatcccat ttattagcta
     2101 gctcagcgcg atcatatagc tctaaagtct tatcagtttc acgcgtcata tacacagtag
     2161 cgacgctgtg agcctgtagc agggctttta gtcgcaaacc tatgtctagt accccgtcgg
     2221 cttcaatgta ccccgtaggg cctacattgc ttcgatactt gccgccatgg ccggggttaa
     2281 gtactatcct cattactact tacctccctt actttttaac tgttccaacg catcagttat
     2341 tcctggaggt accagcacac cgatcttgcc caggttttct aaaatcgaaa tgccttcgtt
     2401 agcaatgtag aaccagatgg ccaaattccg caggatctcc tgcccgatca caatgtcaag
     2461 catggtagca actgccaccg gcacgaacag catgattttt ttaacaatgc cccagaaccc
     2521 cttatatgag ctaacctctt tacgatgaaa cgcagcgaca acaccagaaa tataatctaa
     2581 tacaacgagt actacaagaa cctgcagagc cacgtcccag cctcctaata gcgtagacag
     2641 ggctccaagc accacggcca ctgccgcctt tagtatgatt aatcctttct ccattacagt
     2701 aacccccttg cttttttgca ctaaaaaacc ggctacttgg ccggctgctc tttttacttt
     2761 actgactcac tgacgtatat ccctcaacca gcacaatcat tttacccaag ccctcattca
     2821 ctgcatagtt cgcttcagtt cctgcgtctg tggtttctac ataaaaggtt cttcgcatac
     2881 ccgatgatag gtagttagtt aagtctccac ttcttatctg tatcttatta gaggtgttat
     2941 taggatctgt accactgtaa gcaagcgtcg ggctccaagc tgatacccca agcacagcac
     3001 ttgtaatgtt tacacctgac cgccagagga cctgcgaatc tccgctattc gctgttcgat
     3061 aatggtaacc ttcggtgcca tccgagtggt ataatctcaa attacgagat tgcttccact
     3121 ttgtagggtg tgaccctaac cccgcgggat tgtcataaaa catcggcatg gcatacaggt
     3181 aaattgtcgc tctcgttacg gtgaagttat caggtatgaa aaccgtaaca cccatacgcc
     3241 cctttgtaag ctcccactcc attaagccta atgaactcca acctccgcta tcgccccaac
     3301 tgccgccgcc gctactctca aactgaaaca ccgacagcac tccctgcccc ccaacgacta
     3361 tatccccacc tacccacaca tcaccggaga acttacccgt gatggcagct aaatcctcca
     3421 ccgcaagcat ttcagcgaca atggattttg ccctaataag ctccgtgtta agaaaaccac
     3481 caattattat ggtgtctcct agcatggcca cttgaacctg atcggaatac gcaagtctgc
     3541 ccaggtcttt gtttagctgt tcgcgcaggt atgctatttt gtaatttact gattcggggt
     3601 tattataact ccttgccact tgtagccaga ttggcatttt atcacctgcc tttgcgacgc
     3661 attattgtcc taatatgtcg tattactcta ttgcttctat agcaaaccag atattgtacc
     3721 aatctgttac gtttaggcta cccccagatg tttgcatagc aaatatttca aaatagtcac
     3781 ccgctaccac ctctaaaact ggggacacta cgtgggctat tgcgcctgtt ccactaacag
     3841 gtattacggt agctgttgct ctgccgttat aattaccgtt tccattttta tatatatata
     3901 cttctcgttt acccgttgtg ttttcctgaa aacctacact agctttaact attattttat
     3961 taactcccga cgggatagtg agtcttgttg ggagtcctgc actatatata ttaccaatat
     4021 tgtagactac atctgtccat gttaaagctg tcgcagtatt atgagggata gattgtgcag
     4081 taccctttct taacaatgca cctttaaaag ctttttctgc caagtacgta tctaacgcat
     4141 cccagttctg gtttcgaccc gccagatcgt cctcgatttt attgatcata ctttctttat
     4201 ataaagccat cacatcacct catttatcac ttgttgccac gtcataccag cgtaacgttg
     4261 ccatgtgtag atttcttcgg cttcgtaggt tgtctcaggc gtaatatgca gcttcccctt
     4321 gtagtcgagc gtatctccat cgaatagtag ctgatagtcc tcgacaccat taccaattaa
     4381 aaaagtgcca ttcgttaggt cgaatcgcac cttaccgcct ttgagtattc ctgctatgat
     4441 ttcatcggct acaaagcctg cccccgtgcc aaacgctctc cagtcgtact caccatctgg
     4501 gagctttgca tttgcaagca taaagccttc accggttagc atcatggcag ctgtcttgtc
     4561 gttattttca aaaatcagat tgccattatc gtctgtatgc caaccgcttt gtgtagatag
     4621 cagctggtgt tttagaatgt caataacgcc gttgagatat tgagtattaa tcccatcgct
     4681 gtcgattatt tttgccctat cccaaacgcc ctgcctgcct ctaaaattgc tgatatattt
     4741 ctcctgctca ttgattttgt caacaaagct gggcataaag ttgccgagcg taatgtcgtt
     4801 gttttctggc tctagcaagt ctctaactat ttttattgtc cttgctttta gtcgcaactc
     4861 cggcttaaat tctttgtcaa ttactgcaac ataatcgccc agctctacac cttcgtgctc
     4921 aatgccaaaa gcttttaagt ctatcactgt tgcctgatag gtaatgagtg gggcagatac
     4981 ttcctctagt tttgccagtg tcaaagcaag taactcgtgc ggatcctcgc agtcgtcaaa
     5041 ctctaccttg tcgaaaacgt gtgacttcgt gccgtctgga ttattcctgc cccagatggc
     5101 tttagcgtca ttgttttcca cgtaggcttt gccatcgttt atatcagcaa agtctattct
     5161 cctgccgtaa ccgccggtag gttcgcctgt ttcgtctact atttcctcac ccttgccata
     5221 accgtacagg gcagtgataa tatcatcacg atgtacggtt tttgtgacgg acagcaggtc
     5281 tttggtgtag gtaaagcgct taccataatc cccgccgcga ttggccagta gatcgacata
     5341 c
//
LOCUS       DASRMS010000006         7797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_672277, whole genome shotgun sequence.
ACCESSION   DASRMS010000006 DASRMS010000000
VERSION     DASRMS010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_672277"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..358
                     /locus_tag="VFC74_00270"
     CDS             26..358
                     /locus_tag="VFC74_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HZK23808.1"
                     /translation="MKIKNLKHDAYSDGVLHYGKMTIVYGAGNKKIGETFEPEGKLFF
                     SVMSARDSDNITANAFGYTIDKKLKTHYCPQLKTSHKVKVDEEIFDIKSLDSDRRNSY
                     LYLQRAGV"
     gene            362..718
                     /locus_tag="VFC74_00275"
     CDS             362..718
                     /locus_tag="VFC74_00275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23809.1"
                     /translation="MAKWTVTNETIVSKLEEFGLPVEYGNVSQEVLESYNFFYYREDS
                     LEGTGKILSQTLNIYYVSLNQESLMEPEIIEALTSIKLDFRRATYERLQIEGTTNFVD
                     VVTFICTRKLKVKSCG"
     gene            711..1106
                     /locus_tag="VFC74_00280"
     CDS             711..1106
                     /locus_tag="VFC74_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23810.1"
                     /translation="MARFELKAEDLKRLEEKIGRLQEQAEEEINKILHTETVRDVSNS
                     IQQLINVSKKKKGTHAKHGKPFKTEPFNLGFFIRNYPSYGYLVFPDEGRGIRNQIKQD
                     FTGRGIERERPRVVDRLLEVLTKRIEEEL"
     gene            1106..1684
                     /locus_tag="VFC74_00285"
     CDS             1106..1684
                     /locus_tag="VFC74_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23811.1"
                     /translation="MVTEIKEFDAMKVSKVALQYIGDEAPAVESFECLGSIGGETEMR
                     TIVKRCGGAVRKSKSIPVSHTLTLSGHVPVKTLRSIYGLSNDELKAGVYSYGLDSKGL
                     DFCLTADVVDEFEDVTKLVAFPNCSSLTGLKYTIDNDADEVAYVELEFDARPDDDKQF
                     YYEAFVGEVDAAIASGWHTAFTRELVEEIVAG"
     gene            1696..2196
                     /locus_tag="VFC74_00290"
     CDS             1696..2196
                     /locus_tag="VFC74_00290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23812.1"
                     /translation="MIERVYLYDLEIVEIEGEFKEIKRNAEVLPCMLTNHALYVGKRD
                     GLLETSLTSELYNIIKVFQGLGVNPKNIKEEDIVKFGEEAYKGIIEVTDEDKIARVIY
                     LGLIGANPQLSYSLEDFILKYHDGMEEKVETYMNLINSLASRDNQFKAEFEKKTSQRR
                     EKGEKK"
     gene            2375..4600
                     /locus_tag="VFC74_00295"
     CDS             2375..4600
                     /locus_tag="VFC74_00295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HZK23813.1"
                     /translation="MAKKSEIQIEFKVINAEFQKAMKEMSGEVTKLNREFKLEQEQLR
                     LNGTEKQKLESRVKYLTDRYKLQQDEVKATKEHLDKARESFGENSKEVQTLENRLLEA
                     QRKEAYFANELQVATKALAEQNDKFKQIGKSMQEFGKKMQDIGKKMTDMGKNLSTKVT
                     LPIVGVGVAASKLGIDFEATMSEVQAVTNATGDELQALEGAAREAGATTNKSARDAAD
                     ALKYMGLAGWDVADSQKALMPMLKLSSAGNMELGRTSDLVTDSMSALGLEIGDMDGYL
                     DILAQTSRNSNTDIDKLSEAFVAVGGRLNLLGVPTNDAAVALGILGDNGIKGSEAGRG
                     LNAVLTNLTAPTGKAQKALDELNISAFDSNGEFIGLEETFQLVKDATNGMTQEQQNMY
                     YSMIAGKEHGKTFNAILGSLGDGWDSLSDDVANAEGALDEMYDTMTDNTAGAIDELKS
                     GLEELGLKIYDNLQPTIESLIGIVQGLTDGFNNLSPEAQENIVKIGMMVAAIAPALVI
                     GGKLIDTVGKTADRFGKLIPVIAKTGTFIAGLSAPVLIAVGVITALIAIGVALYKNWD
                     TAMKWGRKLKDSVSTSFSNIKKSIADSIDAAKESVRIAIEKIKSFFKFEWSLPKLKMP
                     HVKISGSFSLVPPRVPKFSIDWYKEGAIFTKPTLLSTTTGLKGVGEAGAEAVLPITKL
                     AGLIKDIIKSDTNLSDAYAKTVAVLEVIVPVSLDGRQIAVATAPHMNVELGKIADRGR
                     R"
     gene            4604..5311
                     /locus_tag="VFC74_00300"
     CDS             4604..5311
                     /locus_tag="VFC74_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="distal tail protein Dit"
                     /protein_id="HZK23814.1"
                     /translation="MIFNNIDMCDYFTANDVRRDILPPVAVATKAIPGRVGERFIKRN
                     LGMGIIEVDITVEGFLLSEMREKVREIAGILNTDEEKQLIFSDEPDKYYMAILNGDTR
                     LKEIVSVGEGMLTFLCPDPLAYSLDAVTASLANKVYNGGTAEATGVITVNVTDSVDYV
                     QAKLKSTGEIVRIGDDFVAGDTVIINLAQEHAEKNGHSIMAKVALASDFFDLPTGEFE
                     IEVTGGTGTLEFRERWL"
     gene            5312..5653
                     /locus_tag="VFC74_00305"
     CDS             5312..5653
                     /locus_tag="VFC74_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23815.1"
                     /translation="MRIKGTDIYMTRGDTENLYVSCVDEDNVPIDFVAGDTVYLTIKK
                     STRTETVELQKVVTEFEDGGALIEFAPEDMKLFSFGSYVYDIQLTQADGTVTTIVKPS
                     KFVIEDEVTDD"
     gene            5646..7400
                     /locus_tag="VFC74_00310"
     CDS             5646..7400
                     /locus_tag="VFC74_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23816.1"
                     /translation="MTKLIGVLENKTKVLTGQLEPKNHIQVAVISGGKPGKPGSDASV
                     TSENIEGALGYVPADKQELDTHENDYAQYKKPFHYDPIKQEYVNLDKFWDTLRNGKIY
                     TVEFNQFDTSPSPLGTKKDDNANLVCEPSTNTVKGRNDYENIGLFMSFDVNAYVDENG
                     DYHVTAIKGDGRFANDGTMGDVYVMAMPGYQKRYATDEAWGVSYSDTMYPGFEILDEA
                     VKPDGTIKPYLLHAKYVAGRNHHEGNNLASISGVNPEYVNMSHNGQITEFGEKGSQYS
                     GKTSHDDYYVQLMFWLKYATTHSDSVMKGCQLYYLQYTNLVPETGVNRVIITNAQANN
                     LLLGSTVSIGDYGDGSINADRAAAQNFNIANRVKVTDIVDLGDGRSAVYVDVDSFDTT
                     LSTTIATYPWASGACDDVLGQDGSPYSNSSGKEPFIINGIEMMVGGYEVIQNLIIYNN
                     NTDSDDYKIQVFVCYDCTKYATSPNEYYNLVAHELVQTDQVWKYASKLDIDNNHPSVL
                     IPIEAEASSTTGFADGVHTSTPTTSYRVWLSLGRLYDGALAGFRFLDAINSLAGTTWR
                     VLGRLSATGQSRRRAGVS"
BASE COUNT         2544 a         1462 c         1906 g         1885 t
ORIGIN      
        1 ggcattgaag aaatgaaggc tgaagatgaa gattaaaaat ctaaaacatg acgcttattc
       61 tgacggagtt ttgcattacg gtaaaatgac cattgtttac ggtgctggta ataaaaaaat
      121 aggcgaaaca tttgagccag aaggcaagct gttttttagt gtgatgagcg ccagggattc
      181 tgataacatc accgccaatg cctttggcta caccatcgac aaaaagctaa aaactcacta
      241 ctgcccgcaa ttaaaaacat cacacaaagt aaaggttgat gaagaaatat ttgacatcaa
      301 aagcctagat agcgaccgca gaaatagcta cctctacttg caaagggcag gtgtatagtc
      361 tatggcaaaa tggacagtta caaatgagac aatagtaagc aagttggaag agtttggctt
      421 gccggttgag tacggcaacg tcagccagga agtgcttgaa tcctataact ttttctacta
      481 ccgcgaagat tccttggagg gtaccggcaa aatcttgtct caaacgctaa acatttacta
      541 cgtgagcctt aatcaagaaa gtttgatgga accagaaata attgaggcat taacatctat
      601 caaacttgac ttcaggcgtg caacctatga gcgcttacag attgaaggca cgaccaattt
      661 tgttgatgtt gtaaccttta tctgcacacg gaaattaaag gtgaaaagct gtggctaggt
      721 ttgagctaaa agcagaagac ttgaagcggc tagaagaaaa gattgggcgt ttacaagaac
      781 aagcggaaga ggaaataaac aagattctcc atactgaaac tgtgagagat gtaagcaact
      841 caatccaaca actaattaat gtgtctaaaa agaagaaagg cactcacgcc aagcatggca
      901 aaccttttaa aacagaaccg tttaatctag gctttttcat taggaactac ccctcctacg
      961 ggtatctggt gttcccagat gaagggcggg ggataagaaa ccagataaag caagacttta
     1021 ccgggcgtgg catcgagcgt gagcgtccta gggtagttga caggctccta gaagtattaa
     1081 caaaaagaat tgaggaggaa ttgtgatggt gacagaaatt aaagagtttg atgcaatgaa
     1141 ggtcagtaaa gttgcattgc aatacatcgg tgatgaagca ccagctgtgg aatctttcga
     1201 gtgcctcgga tccattggcg gtgaaacaga aatgcgtaca atcgttaaaa ggtgcggtgg
     1261 cgcggtcaga aaatctaagt ctatccccgt ttctcacaca ctgacactat ctggtcatgt
     1321 gccagtcaag acattgcgca gcatttatgg cctatcaaac gacgaattaa aggctggtgt
     1381 gtactcttac ggactggata gcaaaggctt agatttttgc ctaactgcag atgtggtaga
     1441 tgaatttgag gatgtaacaa agctagtggc tttcccgaac tgctctagcc taactggcct
     1501 aaagtacaca attgacaacg atgctgacga agtggcctat gtagagctag agtttgatgc
     1561 aagaccagac gatgataagc agttttacta cgaggcattt gttggcgaag tagacgctgc
     1621 tattgcaagc ggctggcaca cagcctttac gcgtgaatta gttgaagaaa ttgttgcagg
     1681 gtaagggtgc tgcagatgat tgagcgtgta tacctttacg acctagaaat agttgaaatt
     1741 gaaggcgagt ttaaagaaat caagcggaat gcggaagtct tgccgtgcat gctaaccaac
     1801 cacgcccttt atgtcggcaa aagagacgga ctgcttgaaa catcccttac cagtgaactt
     1861 tacaacatca taaaagtatt ccaggggttg ggcgtcaatc ctaagaacat caaagaggaa
     1921 gatattgtca agtttgggga agaggcttat aaaggcataa tagaggtcac ggacgaggac
     1981 aagatagcaa gggtaattta tctagggttg atcggtgcta acccgcaact gtcgtatagc
     2041 cttgaagact ttatcctaaa atatcatgac gggatggaag aaaaagttga aacatatatg
     2101 aacctaatca acagccttgc ctccagagac aaccaattta aagccgagtt tgagaaaaag
     2161 acctctcaaa ggcgagagaa aggcgaaaaa aagtaataag tcctcaacta aaatttgagt
     2221 gcgtagaaga caagtactcc ttctatgtcc ttgaactcaa tttaggtgag gacttttttt
     2281 ggcataaacc gattgctatg gttgagcgcg ctgcagacaa cattattgcc atcagaggct
     2341 ggaaaaataa cccgcggaca aggtaggtga tacagtggct aaaaaaagcg aaatccaaat
     2401 tgaatttaag gtaattaacg ccgagtttca aaaagcaatg aaagaaatgt cgggggaagt
     2461 taccaagcta aatagggagt ttaaactaga gcaagaacag ctgcggctta acggtacaga
     2521 gaagcagaag cttgagagcc gagttaaata tttaactgac agatataaac tgcagcagga
     2581 cgaagtaaaa gccacaaagg aacaccttga caaagcgcgg gaatcctttg gcgagaactc
     2641 caaagaagtc cagacgctag agaacagact gctggaagcg caaaggaaag aggcctattt
     2701 tgccaatgag ctgcaggtag caacaaaggc actggccgag caaaacgata agtttaaaca
     2761 gatcggtaag tccatgcaag agtttggcaa gaagatgcag gacattggca agaagatgac
     2821 tgacatgggc aaaaatcttt ctacaaaagt gacattacct atcgttggag taggagtggc
     2881 agcctctaaa ctaggaatcg actttgaagc caccatgagt gaagtgcagg ctgtgacaaa
     2941 tgcaacgggc gatgaattac aagcactaga aggcgctgca agagaagccg gagcaacaac
     3001 taataaatca gctagagatg cagcagatgc tttaaaatat atgggacttg caggctggga
     3061 tgtagcagat tctcaaaaag ccttaatgcc aatgctaaag ctttcaagtg ccggaaatat
     3121 ggagctaggc agaacatctg atttagtaac agacagtatg tcagccctag gtctagaaat
     3181 tggggatatg gacggatact tagatatctt ggcacagaca tctagaaact ccaataccga
     3241 cattgacaag cttagcgaag cttttgttgc agttggtggc agattaaatc tattaggagt
     3301 tccaacaaat gatgcggcgg tagcgcttgg aatactggga gacaacggaa taaagggcag
     3361 cgaagcaggc agaggactta atgcagttct gacaaatctt acagcaccaa ctggaaaagc
     3421 acagaaagca ctagatgagc taaacatatc cgcatttgat agcaatggtg aatttattgg
     3481 actggaagaa acttttcaat tagtaaaaga tgccacaaat ggaatgacgc aggaacaaca
     3541 aaacatgtat tactctatga ttgcaggcaa ggaacacgga aagactttta acgcaatact
     3601 gggcagtttg ggagacggtt gggatagtct atctgatgac gtagcaaatg ccgaaggtgc
     3661 actggatgaa atgtatgata ctatgactga caacactgcc ggagcaattg atgagctaaa
     3721 atcgggactg gaggaattag ggctcaaaat atatgacaac ctacaaccta caatcgaaag
     3781 cttaattggt atagtacagg gcttaactga tggatttaat aacttatctc ctgaagcgca
     3841 agaaaatata gtaaaaatag ggatgatggt tgcggcaatc gcccctgcct tggtcatagg
     3901 tggcaaactg atagatacgg taggtaagac ggcagatagg tttgggaagc ttataccggt
     3961 cattgctaaa accggaacct ttatagctgg cctaagtgca ccagtattaa tagcggtggg
     4021 ggttataaca gcactaatag cgatcggcgt ggcactatat aaaaattggg acaccgccat
     4081 gaagtggggc agaaagctaa aagacagtgt ctcgacatcc ttttctaaca tcaaaaaatc
     4141 catcgcagat tctattgacg ccgcgaagga gagcgtccgg atagccatag aaaagataaa
     4201 atcattcttt aaatttgagt ggtcactgcc aaaactaaaa atgccgcacg taaaaataag
     4261 cggatcattt agcttggtgc ctcccagggt gccgaaattc tcaatcgact ggtacaaaga
     4321 gggggcaatt tttactaagc cgaccttgct tagcactacg acagggctta aaggtgtagg
     4381 cgaggctggt gctgaagcag tactgcctat tactaagctg gcagggctca ttaaggacat
     4441 catcaaaagc gacacaaact taagcgatgc ctacgcaaag actgttgcag tacttgaagt
     4501 aattgtacct gtaagcttag acggcaggca aatagccgtg gccactgcgc cgcatatgaa
     4561 tgtcgaatta ggcaaaattg cagatagggg gaggagataa gtcttgattt ttaataacat
     4621 tgatatgtgc gactacttta cagccaatga tgttagacgc gacatcctcc ctcctgtcgc
     4681 cgtagccaca aaagcaatcc ccggcagagt aggagagaga tttataaaaa gaaaccttgg
     4741 catgggcatc attgaagttg acatcactgt cgaaggtttt ttattaagtg aaatgaggga
     4801 aaaggttaga gaaattgctg gaatcttgaa cactgacgaa gaaaagcagc taatctttag
     4861 tgacgagccg gataaatact acatggccat tttaaatggc gacacaagat taaaggaaat
     4921 agtatctgtc ggtgagggta tgctgacctt tttatgccca gaccctttag catacagcct
     4981 tgatgcagtc acagccagtc ttgctaataa agtctacaac ggtggcacag cagaagctac
     5041 aggcgttatc acagttaatg tgaccgattc ggtcgattat gtccaggcca agctaaagtc
     5101 tactggcgag attgtcagga taggggacga tttcgtagct ggcgacacag tgataatcaa
     5161 cctggcgcaa gaacacgcag aaaaaaacgg tcacagcatc atggccaagg tggcgcttgc
     5221 aagtgatttt tttgacctgc cgacaggtga atttgagata gaagtgacag gcggcactgg
     5281 cacactggag tttagggaga ggtggttata aatgagaatc aaaggcacag acatatacat
     5341 gacgcgcggc gatactgaaa acctttatgt gtcttgcgta gatgaagata atgtcccgat
     5401 tgattttgtg gcgggtgata ctgtatattt gacaatcaaa aaaagtacaa gaacagaaac
     5461 ggtggagctg caaaaagtag taacagagtt tgaagatggc ggcgcgttaa ttgagtttgc
     5521 gccagaggac atgaaattat ttagcttcgg cagctacgtc tacgacatcc aactaaccca
     5581 ggctgatggc acagtaacga ctattgtcaa gcctagtaag ttcgtaatag aggatgaggt
     5641 tacagatgac taaactaatt ggagtgttag aaaataaaac taaagtctta actggtcaac
     5701 ttgaaccaaa aaatcatata caggtagctg taattagtgg tggtaaaccc ggtaagccag
     5761 gtagcgatgc gtctgtaaca agcgaaaaca tagagggcgc actgggctat gtgccagcag
     5821 acaagcaaga gcttgatacg catgagaatg attatgcgca gtacaaaaag cccttccatt
     5881 acgaccctat aaaacaagag tacgtaaacc tagacaaatt ttgggatacc ttgcgcaacg
     5941 ggaaaattta caccgtagaa tttaatcaat ttgacacatc tccttctccc ctcggtacaa
     6001 aaaaagatga taatgctaat ttagtctgtg agccatccac taacacggtc aaaggcagaa
     6061 atgattatga aaatataggg ttatttatgt catttgatgt aaacgcctat gtggatgaaa
     6121 atggcgatta tcacgtgacc gccataaaag gtgatggtag atttgctaat gacggtacca
     6181 tgggcgatgt gtatgtaatg gctatgcctg gctaccaaaa aagatatgca acagatgagg
     6241 catggggagt gtcttattct gatactatgt acccaggttt tgagatttta gacgaagctg
     6301 tgaaacccga tggcacaatt aaaccttatc tactccatgc taaatatgta gcgggcagaa
     6361 atcatcatga gggtaataac ctagccagca ttagtggtgt aaatccagag tatgtaaata
     6421 tgtcccacaa tggacagata acagaatttg gcgaaaaggg ttcgcagtat tcagggaaaa
     6481 catcacatga tgactactat gtacagctaa tgttttggtt aaaatatgcg acaactcatt
     6541 ctgactcggt gatgaaaggg tgccaattat attatttaca atacaccaat ttagtgcctg
     6601 aaacgggtgt aaatagggtt attattacta atgcacaggc taataattta ttactcggtt
     6661 caacggtttc tataggtgat tatggtgatg gtagcattaa tgcagatagg gcggcggcac
     6721 aaaactttaa tatagccaat agagttaaag ttacagatat agtagatttg ggagatggta
     6781 gatctgccgt ctatgtagat gtagatagtt ttgataccac cttatctact acaatagcta
     6841 cctacccttg ggcaagtggg gcttgtgatg atgtattagg tcaagatgga tctccttact
     6901 ctaattcatc tggtaaggag ccttttatca ttaatggcat tgaaatgatg gttgggggct
     6961 atgaggtaat acaaaacctc attatttaca acaataatac tgactctgac gattataaga
     7021 tacaggtgtt tgtttgttat gattgcacta aatatgcaac atctcctaac gagtattata
     7081 atttagtggc gcatgaatta gtacagactg atcaggtgtg gaaatatgct agtaagctag
     7141 acatagacaa taaccacccg tctgtattaa tacccatcga agcagaagca tcttccacta
     7201 ctggctttgc tgatggagtg catacaagca cacctacaac atcttatcgg gtgtggctct
     7261 ccctcggtcg tctgtacgat ggggcgcttg cgggatttcg tttcttggat gcgattaact
     7321 cgctggcggg cacgacttgg cgcgtcctcg ggcggctttc tgctactggg caaagccgta
     7381 ggcgtgctgg ggtgagctag gcttgcctag cgaggggctt gccccttatt gtgacaggta
     7441 actttgtaat taatatatat aaggacttgt ggtgcggctc gggtgtggct ctccctcggt
     7501 aatctgaaca atgggacgaa tgcgggatta cgttacttgg ctgcgaatca ctcgctggcg
     7561 ggcacgaatt ggaacatcct cgggcggaac tctgaaaatg attttagctg caccacaggt
     7621 atagctatga cacataagga acattaagcg caactaggga ggtgagcaaa ataaaattcc
     7681 tgctatttga taaaaacgag aaccttatat ccaccctaaa agatgtcacc atgaccaagc
     7741 acaccgaaga aatcaacggc gagaacaccc tagaactaga aactctggac gaagtag
//
LOCUS       DASRMS010000007         2330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_270192, whole genome shotgun sequence.
ACCESSION   DASRMS010000007 DASRMS010000000
VERSION     DASRMS010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270192"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..187
                     /locus_tag="VFC74_00315"
     CDS             <1..187
                     /locus_tag="VFC74_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23817.1"
                     /translation="GRQGLTLAEVVKCRHGQVGGTVIQMQGAYARFVNAAEGDYDRVT
                     GGTGSKTEYLDKTFKSR"
     gene            287..871
                     /locus_tag="VFC74_00320"
     CDS             287..871
                     /locus_tag="VFC74_00320"
                     /inference="COORDINATES: protein motif:HMM:NF018772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HZK23818.1"
                     /translation="MSKVNLVRNGRSFAPATEQDEDLLKRVRTGEVIACDFTKPRNPQ
                     FHRKFFALLNLAYEYWVPDGGLMPQQELFGITGLAKYIGAKMPGVDAQELAKEYVTMQ
                     IAGRSEKYPAVEKDFELFREWIISESGHSELKQTPRGIVKKAKSISFARMDETEFSGL
                     YKSVFNVAWRHMLQASFASEAEAMAAADSLGEFA"
     gene            1601..2242
                     /locus_tag="VFC74_00325"
     CDS             1601..2242
                     /locus_tag="VFC74_00325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23819.1"
                     /translation="MKPKTKKLVCGIGWNDADYVVNKKETIGYEDGKQRFKLVWACKY
                     YSTWASMLSRCYSAKAQKKRPTYAGCTVSEDWHTFSKFKSWMEKQNWEGNQLDKDLLF
                     EGNKLYSADTCVLVTPMVNSFTIDCRAVRGEWQVGVSWDKIKNKFQANCRNPFTRKQE
                     NLGRFDSEQEAHNAWKKRKLELAHELAAIQTDQRVAKALIDRYSKPQISENEL"
BASE COUNT          698 a          476 c          611 g          545 t
ORIGIN      
        1 tggtcgtcaa ggtcttacac ttgctgaagt agttaaatgt cgtcacggcc aagtgggtgg
       61 aactgttatt caaatgcaag gtgcctacgc aagatttgta aatgccgcag agggtgacta
      121 tgaccgagta acaggcggta ctgggtcaaa aactgagtat ttagataaaa cgtttaaatc
      181 taggtaatct gctataataa ccaagcggct agggcatcga acccgaacac cagctagtca
      241 ctgactgccg ctttaacctt tcgactagcc ttgactgggg ttcgttatgt caaaagtaaa
      301 tctagttcgt aatgggcggt cattcgcgcc agctaccgag caagacgaag atctattaaa
      361 gcgcgtgcgc accggtgagg ttatcgcgtg tgattttacc aagccgcgca accctcaatt
      421 ccatcgcaag ttcttcgcgc ttttaaatct tgcttatgag tactgggtgc cggacggcgg
      481 cctaatgcca cagcaggagt tgttcggcat cacggggctg gcaaagtaca ttggcgcaaa
      541 gatgccaggt gtagacgctc aagagctggc caaagaatac gtcacgatgc agatagccgg
      601 gcgatcagaa aagtacccag cagttgaaaa agactttgag ttgttccgtg aatggataat
      661 ttccgaatcc ggccatagcg agctaaagca aacgccgcgc ggcatcgtaa agaaagccaa
      721 atcaatcagc tttgcaagaa tggatgagac cgagtttagc ggcctgtaca aatctgtttt
      781 taacgtggca tggcgacaca tgctgcaggc cagttttgca agcgaggctg aagctatggc
      841 agcggcagat agtcttgggg agttcgcgtg actaatcaga ggaagaggaa gtgtaagttt
      901 tgcggcgagt atgttcgcgg ttggataagc gtacctgccg gtacgttttg cgattacgac
      961 catgctatac agttcgctaa tcagcccaag ataaaaaagg ctacagaaaa taaactacac
     1021 gctgagcgaa agaagaggct gcgtgacaat gaccgcggct attggctaaa gagagcgcag
     1081 gcagatttta acgcatacat acgcaaacgt gatgagcatt cgccctgcat cagctgccag
     1141 cgccatcata ccggccagta tcacgctgga cattatcgtt cggtcggcgg caatcctgag
     1201 ttgaggtttg agccgctgaa ctgccacagg caatgtgccc cgtgtaactc gcatctgagt
     1261 ggaaacctga ttaattatcg gataaaccta atcaagaaga ttgggcaagc caaggtggac
     1321 tggctggaag ggccgcacga gccaaagcgg tacaccatcg aagaccttaa aaacataaag
     1381 gcacgataca ggaaaaaact aagagagata aaaagcggct aagtcaggag taaaagttgc
     1441 aagccttggt ggtagtgcgt ataattagag ttgttgtaag cggcgtagta accgttaaag
     1501 tctaaagcta ataaaggtct gtaccatttt tctcgaggct agcttttgcg cctttactac
     1561 cgagactgat ggcacagacc ttttttattg ggtaaacggt atgaaaccta aaactaaaaa
     1621 actggtgtgt ggcataggct ggaatgacgc tgattatgtt gttaacaaga aagaaactat
     1681 tggatatgag gatggaaaac agagatttaa gctggtttgg gcttgcaagt attacagcac
     1741 atgggcaagc atgttgagta ggtgctactc tgctaaagct caaaagaagc gccctactta
     1801 tgctggctgc actgtctcag aggattggca cactttcagc aagtttaaat cttggatgga
     1861 aaagcaaaac tgggaaggca accagttaga taaagacctg ctttttgagg gaaacaaact
     1921 gtacagcgca gacacctgcg tgcttgtaac cccaatggtt aactcgttta ccattgattg
     1981 cagagcggtg cgcggtgagt ggcaggttgg ggttagctgg gataaaataa aaaataaatt
     2041 tcaggctaac tgcagaaatc cttttacccg gaaacaagaa aatcttggac gttttgattc
     2101 cgaacaagaa gcccataatg cttggaaaaa aagaaagcta gaactagccc acgagctggc
     2161 tgcaatccaa acagatcaaa gggtagctaa agcattgatt gatcgctact cgaagccaca
     2221 aataagcgag aatgaattat gaaaaatata ttaattggtt tagttttatc ggttgccgtg
     2281 agtgcgccag tgtttgctgc tgagcagatg agtaaagaac agttttgcgc
//
LOCUS       DASRMS010000008        17285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_337313, whole genome shotgun sequence.
ACCESSION   DASRMS010000008 DASRMS010000000
VERSION     DASRMS010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 17285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17285
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_337313"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            156..677
                     /locus_tag="VFC74_00330"
     CDS             156..677
                     /locus_tag="VFC74_00330"
                     /inference="COORDINATES: protein motif:HMM:NF039198.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HZK23820.1"
                     /translation="MNILLNAFFIFFARVSDVTLSTVRILMIMRGRSISAAIIGFAEV
                     SIYVLALSRVIGSLESPILVVAYALGFSAGTLTGGLLEERLAIGYATAQIISLEQSRQ
                     ITEQLREDGFGVTIIEGSGRAGTHEILHVLLKRKDIPDLLNLIKQADQQAFVSIMDTR
                     KILGGYFRRIKAK"
     gene            693..1496
                     /gene="murI"
                     /locus_tag="VFC74_00335"
     CDS             693..1496
                     /gene="murI"
                     /locus_tag="VFC74_00335"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZK23821.1"
                     /translation="MNRNHAIALLDSGIGGLTVVREINKQLPQERIVYFGDTARMPYG
                     PRPQAEVRGFASQIIRFLLTQDVKLVIVACNTATAAGLPAYQQAYDLPIIGVIEPGVR
                     AALHQTRTKRIGVIGTPGTIASKAYEDSLRTHDATVEIFSQACPLFVLLVENDLVNTP
                     AAVCVAEEYLRPLREAGVDTLILGCTHYPLMAEVIGKVMGPEVTLISSAAETAQEAKA
                     ILSQQQSLRDDLAEPLRHRFFVSGNPKPFTQLAETLLAYQLETYQVLLT"
     gene            1558..2550
                     /locus_tag="VFC74_00340"
     CDS             1558..2550
                     /locus_tag="VFC74_00340"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZK23822.1"
                     /translation="MRKGLSLFLVLLLVFCSGCSLLPEKKEVAEQAEDRLPEPPPVQQ
                     PNLRETMLYLPNETHQFLLPLRVYIPWEEGIAKATLTYCTAGNLLPEVAALGVAPLLP
                     AGTAITGLSVREGLARVDFSEEFLDYLPEQEEQIINGLTYTLTEFSTIDAVEILCAGQ
                     QLQTLAATAPTPPLSREFVLNLEAPVAADDSASMQRITLYFLHEVGSRIFYVPVTRLV
                     AETAELLPTIAKELLLGPKPGTSLLTAVPSGLTLKEITTEGSKVVLHLSGELSADGGQ
                     LAADLLRQQLALTFTEIHGITEVAVLCNDKPPELDSGVDFPASFGRPKQWNLVQ"
     gene            2640..3356
                     /gene="rph"
                     /locus_tag="VFC74_00345"
     CDS             2640..3356
                     /gene="rph"
                     /locus_tag="VFC74_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HZK23823.1"
                     /translation="MMRIDGRQPTQMRPLIITPHYLKAPEGSVLIEFGETKVICTASV
                     EEKIPPFLRGANQGWVTAEYAMLPRATEVRTQRERGKVSGRSTEIQRLIGRALRSVVD
                     LKALGERTIWLDCDVIQADGGTRTASISGAYVALHLALAKLVTAGTLGEIPLVDFLAA
                     TSVGLVDQELYLDLAYSEDSRAEVDMNIVMTGSGKLVEVQGTAEGAPFTRQEMQQMLD
                     LAASGINQIVEVQKQILTGK"
     gene            3358..3969
                     /locus_tag="VFC74_00350"
     CDS             3358..3969
                     /locus_tag="VFC74_00350"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HZK23824.1"
                     /translation="MKIIIATKNNGKLLEFKTLLADLPLTITSLRAYPTIGELAETGT
                     SFAENAALKAETVAQLTGVVTLADDSGLEVDILDGRPGIYSARFAGKQASDQENNMKL
                     LLALRGVAPSARTARFRSVIALAQPTGKTVFAEGVCEGLIAAEPQGCGGFGYDPLFFV
                     PELAQTFAQIPAEVKNSISHRARALAAVKTILADLLAENRQRF"
     gene            4073..4149
                     /locus_tag="VFC74_00355"
     tRNA            4073..4149
                     /locus_tag="VFC74_00355"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4107..4109,aa:Pro,seq:tgg)
     gene            4283..4356
                     /locus_tag="VFC74_00360"
     tRNA            4283..4356
                     /locus_tag="VFC74_00360"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4315..4317,aa:Gly,seq:tcc)
     gene            4407..4482
                     /locus_tag="VFC74_00365"
     tRNA            4407..4482
                     /locus_tag="VFC74_00365"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4440..4442,aa:Arg,seq:tct)
     gene            4576..4652
                     /locus_tag="VFC74_00370"
     tRNA            4576..4652
                     /locus_tag="VFC74_00370"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4610..4612,aa:His,seq:gtg)
     gene            4669..4754
                     /locus_tag="VFC74_00375"
     tRNA            4669..4754
                     /locus_tag="VFC74_00375"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4703..4705,aa:Leu,seq:tag)
     gene            4765..4839
                     /locus_tag="VFC74_00380"
     tRNA            4765..4839
                     /locus_tag="VFC74_00380"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4797..4799,aa:Gly,seq:gcc)
     gene            5078..6397
                     /gene="tig"
                     /locus_tag="VFC74_00385"
     CDS             5078..6397
                     /gene="tig"
                     /locus_tag="VFC74_00385"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HZK23825.1"
                     /translation="MVNLEKIDDSKVVLEIEVPEEKVADALTQAYRKVVKTVSIPGFR
                     KGKVPRPILESRFGVEILYEDALELLVPDAYDSAVEEVGIVALDKPEIDLVRIEKGKS
                     LIFKATVEVTPEVTLGEYRGVAVTKQLREVAETDLENKLTQLQTQHATLEIVEDSTAE
                     NGDIVVIDFTGYQDDEPFAGGAAEGYSIEIGSGQFIPGFEEQMIGMKQGEEKEVEVTF
                     PEDYQEESLAGKPAVFKVKVNEIKRKNLPELDDELAKEISEFDTLAELKEDIMNKLKQ
                     KEEERAQTEVENELIEKVSAATAEFPLPKVLVEREIDRQLSEMEQFLRMQGLTLDKFL
                     ELTNRELAELREEQKPEAEKRVKANLVLDAIITKEGIIATDEELDERVDKFAASYGQD
                     VQTIKSYFAAQGQLDVIRQEIQFRKAIDFLVAAAQITPTAAPIQTEA"
     gene            6409..6990
                     /gene="clpP"
                     /locus_tag="VFC74_00390"
     CDS             6409..6990
                     /gene="clpP"
                     /locus_tag="VFC74_00390"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HZK23826.1"
                     /translation="MSLVPMVVEQTNRGERAYDIFSRLLKDRIIFIGSAIDDTIANLV
                     IAQLLFLESEDPDKDINLYINSPGGSVYSGLAIYDTMQYIKPDVSTICVGMAASMGAV
                     LLTAGAAGKRYALPNSRVMIHQPLGGAQGQAVDIEIHAREILRIREGLNEILAKHSGK
                     PIEQITSDTDRDNFMSAEQAKAYGLIDGVLQKR"
     gene            7012..8274
                     /gene="clpX"
                     /locus_tag="VFC74_00395"
     CDS             7012..8274
                     /gene="clpX"
                     /locus_tag="VFC74_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HZK23827.1"
                     /translation="MFKFNDEKGQLKCSFCGKTQEQVRKLVAGPGVYICDECIELCNE
                     IIEEELSEEMELDLIDLPRPTEIKDILDQYVIGQDESKKSLAVAVYNHYKRINAEQKP
                     DDVEIQKSNILLIGPTGVGKTLLAQTLARILNVPFAIADATSLTEAGYVGEDVENILL
                     KLIQAADYDLEKAEKGIVYIDEIDKVARKTDNPSITRDVSGEGVQQALLKILEGTVAS
                     VPPQGGRKHPHQEFMQIDTTNILFVCGGAFDGIEKIIQSRIGNKGIGFGAEVSGKKEK
                     NIGDILRHVLPQDLTKFGLIPEFVGRVPVVATLDALDKNALMRILTEPRNALIKQYQR
                     LFEMDNVALEFKEGTLEVIAEEAINRETGARGLRSILESTLLDVMYELPTFEDISKCI
                     ITKEVITKQDKPILVTVDKRKKKKEESA"
     gene            8426..10099
                     /gene="lonB"
                     /locus_tag="VFC74_00400"
     CDS             8426..10099
                     /gene="lonB"
                     /locus_tag="VFC74_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease LonB"
                     /protein_id="HZK23828.1"
                     /translation="MLRLAPIIGVVQIIIAIVIGIYFWSLLRSQRSNKVAVKQESNKE
                     MDQLRKLREISLTEPLAEKTRPASFAEIVGQEEGIKSLRAALCGSNPQHVIIYGPPGV
                     GKTAAARLVLAEAKKNAASPFGPEAKFVELDATTARFDERGIADPLIGTVHDPIYQGA
                     GPLGMSGVPQPKAGAVSKAHGGILFIDEIGELHPTQMNKLLKVLEDRKVFLESSYYNA
                     EDKNIPLHIHEIFQNGLPADFRLIGATTRTPEEIPPAIRSRCLEIFFRGLFADEIAVV
                     AANSAKRINFSIDEMALDLITKYAKNGREAVNTIQIAAGIAICDKVTQEITVDDVEWV
                     INASQMSPRPEIKIPPRSQVGYVNGLAVYGPNMGMVLEIEVAAHQAEPGRGQVHVTGV
                     VEEEELGGGQRLIRRKSMAKGSVENVLTVLRDYLEVDAQDYDIHVNFPGGTPIDGPSA
                     GIAIATAIYSALTNIPVLNTVAMTGEISVRGYVRPIGGVVCKVEAARQAGAKKVIIPK
                     ENWQKMFTKLPEVEVVPVENLREVLDHALCRSPQSTRKAQTQMLSASSV"
     gene            10219..12564
                     /gene="lon"
                     /locus_tag="VFC74_00405"
     CDS             10219..12564
                     /gene="lon"
                     /locus_tag="VFC74_00405"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HZK23829.1"
                     /translation="MANDTKQIPLLPLRGILVFPNMVLHLDVGRERSVNALEQAMIED
                     NKILLVAQKEARVDEPTPDDIYSLGTIAQVKQMLKLPGGTIRVLVEGLSRAKVQTFID
                     EEPYFKAEAVELAEDDTKSVEVEALMRSVIYQFEQYIKLSKKIPPETLVTVSSVEEPG
                     RMADLIASHLTLKIQQKQEILEAISPTNRLEKLSNILSHEMEILEVERKINLRVRKQM
                     ERTQKEYYLREQMKAIQKELGEKDERLAEADEYREKISEAQLPDEVEEKALKEVERLE
                     KMPPAAAEAVVIRNYLDWILALPWTTQTEDRLDLGMAEQILDEDHYGLEKVKERIIEY
                     LAVRQLAQKLKGPILCLVGPPGVGKTSLARSVARALQRNFVRLSLGGVRDEAEIRGHR
                     RTYVGAMPGRIIQAMRQGKSQNPVFLLDEIDKMSTDFRGDPSAALLEVLDPEQNNTFS
                     DHYLEIPFDLSHVMFITTANTLYNIPRPLLDRMETIYLPGYTEEDKLQIAKRYLLPKQ
                     IRENGLGKENISISEKTLRKVIRQYTREAGVRNLERQIATLCRKVAKDVVRDNKLKSA
                     ITVQNLHKYLGVPRFRYGIAETNDEVGIATGLAWTEAGGDTLIIEVTLMQGKGKLLLT
                     GKLGDVMRESAQAGLSYIRSRAKELNIAEDFYEKYDIHIHIPEGAIPKDGPSAGITMA
                     TALISALTKRPYKRTVAMTGEITLRGRVLPIGGLKEKVLAAHRAGIKTIILPADNKKD
                     ISEIPENVRRRLELIPVEHLDEVLELALAAVPEEAAEVIEE"
     gene            12561..13154
                     /gene="yihA"
                     /locus_tag="VFC74_00410"
     CDS             12561..13154
                     /gene="yihA"
                     /locus_tag="VFC74_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HZK23830.1"
                     /translation="MKISTAEFIISAVKNTQYPQTDLPEVAFVGRSNVGKSSLLNSLL
                     NRKKLALTSGSPGKTRLINFFLINEAFYFVDLPGYGFARVSQEMKRQWGQIIEAYLTS
                     REPLKLVVQLVDVRHPPTADDLQMYKWLIHFNLPTVIVATKADKIPRGKWQKHLQQIR
                     SGLGLGPQGPVILYSAQTGQGKDELWGVIKQYVLSRG"
     gene            13333..15975
                     /locus_tag="VFC74_00415"
     CDS             13333..15975
                     /locus_tag="VFC74_00415"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HZK23831.1"
                     /translation="MTDKNLAPVYDPSEVEERLYQEWLEKDYFQAPRDLKRKTFNIVI
                     PPPNVTGSLHMGHALDNTIQDILIRRKRMEGFDTLWLPGTDHAGIATQIKVEEHLAAD
                     GLTRYDLGREKFIERVWQWKDEYHERIIGQLYKLGVSCDWSRERFTMDEGCSDAVRQV
                     FVALYHKGLIYRGNYIINWCPRCHTALSDIEVEHEDAEGTLTYIRYPLVDGEGHVVVA
                     TTRPETMLGDTAVAVHPQDSRYQALIGKEVLLPLMNRKIPIIADDYVDPEFGSGAVKI
                     TPAHDPNDFAIGERHQLPSVVVIAEDGTMTQEAGKYAGLDRTEARKQVVDDLEAEGVL
                     LQIEKHQHAVGHCQRCHTVIEPLLSKQWFVSMKPLAEPAIQQVKNGEIRFVPERFTKV
                     YLNWVENIRDWCISRQIWWGHRIPAWYCECGKTIVAREEPQTCPHCGGQALKQEEDVL
                     DTWFSSALWPFSTLGWPEETADLEHYYPTDVLVTGYDIIYFWVARMIFMGLEFMHKKP
                     FHTVYIHGLVRDAQGRKMSKSLGNGIDPLEIIEQYGADTLRFTLITGQAPGHDQRFRQ
                     ESVDNSRNFANKIWNASRFVLMNLGDTTAAELELDSLTDADRWLLHRYNQTVREVNRL
                     MDTYELGEAARTIYEFLWSDFCDWYIELAKIPLYDAQTEAEKQTVRSVLTYVLDRTLR
                     LLHPFMPFITEEIWQKIPHSGKTIMLASWPEYDPALEFSAAHERMATVMEVTRTIRYL
                     RSEVQLPPGRKAQVILNVDSGASAQALAAGEAMLKKLANLEEMTVVRELPEQPQQALT
                     AVVGDIEIYLPLKGLVDIEQEIKRLQKELKKLESEVARAEGKLNNQGFLAKAPQQVIA
                     EERAKAENYRLRRDKVRERIISLR"
     gene            15993..>17285
                     /locus_tag="VFC74_00420"
     CDS             15993..>17285
                     /locus_tag="VFC74_00420"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HZK23832.1"
                     /translation="MKYEEALNWIHGLYRFGSNLGLERVTKLMELLDNPQDKFRSVHV
                     AGTNGKGSTAAFLAQILQAEGLSVGLYTSPYIEAFTNRMALNGHDIGEAELVELVKQV
                     KWAVDELAASAIGQPTEFEVVTALAFTYFAQNAPDWVVVEVGLGGRLDATNVITPTVS
                     VITNIALDHTDVLGGTIAAIAGEKAGIIKPGVPVVTGAQKDEALAVFQQVAAERQSPL
                     YAIDHDFTEEVTASSLAGLTFSYQSPWHQLSDLQVQLLGRHQARNASVAIAARELLPL
                     PFSEEAVREGLRKTMWPGRLEVFSQQPLVMVDGAHNPDGVLALRSALLELLGDRKLRL
                     VIGILGDKAVEEIVGVIAPLAQAGIIVTTPNNPRAADPYVVAELVRKFAGNTPVEVVP
                     EIQAAVQQAVAITQPNEALCVSGSLYTISEAREVLKNIY"
BASE COUNT         4885 a         3512 c         4333 g         4555 t
ORIGIN      
        1 agaccaaggt gcgtgcgctc aactgccatt ttgctcctta caggataccc cctagccagg
       61 ggtaagtgcg cagtgaaata atttattgcc gtataaaata atagggactg gtaaaatatc
      121 tgcttttaca ataactaaag taggtgggtt aaaaggtgaa catattactc aatgcattct
      181 ttattttctt tgctagggtg agtgatgtta cactctccac agtcaggata ttaatgatta
      241 tgcgtggtcg ttccattagt gcggctatca ttggttttgc cgaagttagt atttatgttt
      301 tagctttaag ccgcgtgatt ggtagtttag agagtcccat tttggtcgta gcttatgctt
      361 taggtttttc agcgggtacc ctgacaggcg gccttttaga agagcgtctg gccatcggtt
      421 atgctacagc acaaataatt tcgctggaac aatcgcggca gattactgaa caactgcgtg
      481 aggatggttt tggggtgaca attatcgagg gaagcgggcg ggcaggcaca catgagattt
      541 tgcacgttct cttaaaacgt aaggatatcc ctgatttact taacttgatt aaacaggctg
      601 accaacaagc ttttgtcagc attatggaca cgcgtaaaat cttgggcggc tattttcgta
      661 gaatcaaggc aaaataagcg ttggaggagc atatgaaccg taatcatgcc attgcattgt
      721 tagactcggg gattggcggg ctcaccgttg tacgtgagat taacaaacag ttacctcaag
      781 agcgaattgt ctattttggc gatacggcca ggatgccata tggccctcgc ccgcaggctg
      841 aggtacgtgg ttttgccagc cagatcattc gttttttgct gactcaggat gttaagttgg
      901 tcattgtcgc ctgtaataca gcaacagcag ctggcttgcc agcttatcag caggcatatg
      961 acctgccgat tattggagta attgaaccag gtgtgcgggc tgctttgcac cagacacgta
     1021 caaaaagaat cggcgtaatt ggtacgcctg gcacgattgc cagtaaagct tatgaagatt
     1081 cgctccgtac ccatgacgcc acggtagaga tcttctcgca agcatgtccc ttatttgtgc
     1141 tgctggtgga aaatgattta gtaaatactc ccgcagcggt ctgtgtagca gaagagtatt
     1201 tgcgcccgct gcgagaagca ggtgtggata ctttgattct tggctgcacc cattatcccc
     1261 tcatggcgga agtgattggc aaggtgatgg ggccagaagt cacgcttatt tcttcagctg
     1321 cagaaactgc gcaggaggcc aaagcaattt tgtcgcagca gcagtcttta agggatgatc
     1381 tggccgagcc gctgcgtcat cgcttttttg tcagcggcaa ccctaaaccc tttactcaac
     1441 ttgcggaaac attgctcgct tatcagctgg aaacctacca ggttttactc acttaaagcc
     1501 tgcatatctt tcttcctctg ctcatacatt tcagtaaaac atgaacagga ggaaactatg
     1561 cgtaaagggt tgagcttatt tttagtgtta ctgctcgttt tttgtagtgg ctgctcactc
     1621 ctgcctgaaa aaaaggaggt tgctgagcaa gcagaggaca ggctgcctga accaccgccg
     1681 gtgcaacagc ctaatttgcg agaaacaatg ctttatttgc cgaatgaaac acaccagttt
     1741 ttactgcctc tacgcgttta cataccttgg gaagagggta ttgccaaagc aactttgact
     1801 tattgtacag ctggcaatct actgccagaa gtggccgcac tgggcgttgc tcctctctta
     1861 ccagcgggaa ctgcaatcac aggtctctcc gtgcgcgaag gtttggcacg agtagatttt
     1921 agtgaagaat tcttggatta cttaccagaa caggaagagc agataattaa tggtcttacc
     1981 tacacgctga cagagttttc tacgattgat gctgttgaga tcttgtgtgc aggtcagcag
     2041 ctgcagacgc tggcggctac agcacccacg ccacccttga gccgtgaatt tgttttgaac
     2101 ttggaagccc ctgtggcagc agatgattct gcctccatgc aacggataac gctttatttt
     2161 ctacatgaag taggaagccg tatcttttat gtcccagtaa cgcgcctagt agccgaaaca
     2221 gctgaactgc tgccaacaat agcaaaagaa ctattactag gacctaaacc ggggacgtca
     2281 ctgcttactg ctgtccccag tggtctgacc ttaaaggaaa taactactga gggtagtaaa
     2341 gtagtactac atttatcagg agaattgtct gctgatggag gccagttggc cgcagatctg
     2401 ctgcgtcagc agctggcctt gacctttacg gaaatccatg gcataactga agttgccgta
     2461 ctttgcaatg ataaaccgcc agaacttgac agtggggttg attttcctgc ttcttttgga
     2521 cgcccaaaac aatggaatct ggtgcagtaa tggataataa aggggtaatt gaaaaaataa
     2581 ctatgtaacg ctataatgaa aagacaccag tgaaatttta gcgaaagacg gaggttcctt
     2641 tgatgagaat agatggacgt cagcctacac aaatgcggcc gcttattatc acgccgcatt
     2701 atctgaaagc accagaaggt tcagtgctaa tagaatttgg tgagactaaa gttatttgta
     2761 cggcctcagt agaagaaaaa ataccaccat ttttacgagg tgcaaatcaa ggctgggtaa
     2821 cagcagaata cgcgatgctg ccgcgggcta cagaagttcg tacccagcgg gagcggggta
     2881 aagtttctgg ccgcagtaca gaaatccagc gtcttattgg acgtgctctg cgttcagttg
     2941 tggacttaaa ggcgctcggt gagcggacta tttggctcga ttgtgacgtc attcaagcag
     3001 atggcggcac gaggacggcc tctataagtg gtgcttatgt ggcattgcat ttagcactgg
     3061 ctaaattagt tacagcaggc acgctgggag aaatacccct tgttgatttc ttggcagcca
     3121 ccagtgtagg cctagttgat caagaactgt atctagactt ggcttacagc gaggatagta
     3181 gagctgaagt ggatatgaat attgtgatga caggcagtgg taaattagta gaagtgcagg
     3241 gaactgccga gggagcacca tttacgcgtc aggagatgca gcaaatgttg gatctggctg
     3301 ccagtggcat caatcagatc gtcgaagtac aaaaacaaat cctgactggc aagtaatatg
     3361 aaaataatca tagcgacaaa aaataacggt aaacttttag aatttaaaac tttattggca
     3421 gatttgccgc tcacgatcac ttcattaagg gcttatccca ccatcggtga gcttgctgag
     3481 acaggtacta gttttgcgga aaatgccgcg cttaaagccg agactgttgc ccagcttact
     3541 ggtgtagtaa cgttagctga tgactccggc ttggaggtag atattttgga cggtcgtccg
     3601 gggatttatt cagcgcgttt cgccgggaag caggctagtg accaggaaaa taatatgaag
     3661 ctgctgttag ctttgcgtgg tgtagcaccg tcagcccgta cagctcgttt tcgctctgta
     3721 attgccttgg ctcaacccac gggaaaaaca gtttttgctg aaggtgtatg tgaaggctta
     3781 atcgccgcag aacctcaagg ctgtggtggt tttggctatg atcccttgtt ttttgtgccc
     3841 gagttggcgc aaacttttgc gcaaattccg gcagaagtaa aaaatagcat tagccatcgt
     3901 gcccgtgccc tggcggcagt aaaaacaatt ttagcagact tgctggcaga aaacagacaa
     3961 agattctgac gatcaagaca aaactcaaag taaactagct aattaagcca ataatgcaga
     4021 tattaccttt gacaagtagt agcttaatct ggtatactaa tttttgcaac gtcggggcgt
     4081 agcgcagttt ggtagcgcac ttggtttggg accaaggggt cgcaggttca aatcctgtcg
     4141 ccccgaccac tattgaaagt tagaagttag aggttagaag tcggatgcgc acaactagcc
     4201 aaggctaact ttatttgatt ttataactgg tctgggcaag aggtttagct ctaaccgctc
     4261 accgccagtg accaagctag ttgcgggtgt agctcaatgg tagagcgctg gccttccaag
     4321 cctgttacgt gggttcgatt cccatcaccc gctccaaatt ttgtcgcttg attttatgca
     4381 gttaatcaac tatactagtt aattatacgc ctgtagctca ggggatagag caacggactt
     4441 ctaatccgtg ggccgtaggt tcgaatccta ccaggcgtgc catagacaga tgtcagacct
     4501 cagaggtcga aattgactga atgagagcag agtttcgtct gctgtcgcct ctgaagtctg
     4561 tagtttataa ccatggtggg tgtagctcag gtggttagag caccagattg tggctctggg
     4621 ggccgtgggt tcgagtccca tcatccaccc caattttatt aattttgtgc gggcgtggcg
     4681 aaattggcac acgcgctaga cttaggatct agtgggtttt tcccttgggg gttcaagtcc
     4741 cttcgcccgc accatttata aattgcggaa atagctcagt ggtagagcat cgccttgcca
     4801 aggcgagggc cgcgggttcg aatcccgttt tccgctccag ggtatgaaga cgcctgacaa
     4861 ggcgtttttt ttgttgcctg ataagttatt tcatttagct taagaagtaa atgctttttc
     4921 ccgatctact ttcattttcc ttcatttagt ggtaaaatac ttggcaggct gacaatatat
     4981 atgctataat gcagcgcaag aacaaatggc tacatatccg ttcactgcac tctaattaaa
     5041 ggcatacgat aaaatatggt taaaaggagc atagggcatg gtaaatctgg agaaaataga
     5101 cgacagtaaa gttgtattag aaattgaggt gcctgaggag aaggtcgctg atgctttaac
     5161 acaggcgtac aggaaagtag ttaagactgt gagcataccg ggcttccgta aaggtaaagt
     5221 gccccgtccc atcttagaaa gccgctttgg tgtagaaatt ctttatgaag atgcattgga
     5281 acttttggtt ccagatgcat atgatagtgc agttgaggaa gtcggtattg ttgctcttga
     5341 caagcctgaa attgatctgg ttcgcataga aaagggtaaa tcacttattt ttaaagctac
     5401 tgtggaagta acaccggaag taacccttgg tgaatatcgt ggtgttgccg tcactaaaca
     5461 gctcagggag gttgcagaaa ctgatctgga aaataagtta acccagctgc agacacagca
     5521 cgccacttta gaaatagtcg aagatagcac tgctgaaaat ggtgatattg tagtcataga
     5581 ctttacaggt tatcaagatg atgagccctt tgctggcgga gcagccgaag gctattcaat
     5641 tgaaattggt tcaggtcagt ttattcctgg ttttgaggaa caaatgattg gcatgaaaca
     5701 gggagaagaa aaggaagtcg aagttacctt ccctgaagat taccaggaag agtctctggc
     5761 cggtaaacct gccgttttta aggtcaaagt gaatgaaata aaaagaaaaa atttacctga
     5821 actagatgat gaattggcta aggaaatttc tgaatttgat actctcgctg aattaaagga
     5881 agacatcatg aataagctga aacaaaagga agaagagcgt gctcaaacgg aagtagagaa
     5941 cgaactcatc gaaaaagtat ccgcagctac tgcagaattc cctttgccta aagtgcttgt
     6001 ggaacgtgaa attgatcgtc agcttagtga aatggaacag ttcttacgga tgcaggggct
     6061 aacattagat aagtttttag aattgaccaa tagggaactt gcagaactgc gtgaggagca
     6121 aaaaccagaa gctgaaaaac gtgttaaggc taatttggtt ttagatgcca ttattacaaa
     6181 agaaggaatt atagcaacag acgaagaatt agatgagcgt gttgacaaat tcgcagcaag
     6241 ctacggccaa gatgtgcaaa ctattaaaag ttattttgca gcacaggggc agttagacgt
     6301 catacgccaa gaaatacaat ttcgtaaagc tattgatttc ttggtagcag cagcacaaat
     6361 aacgcctaca gctgcaccta ttcaaacaga agcataggag ggatttaaat gagtttagta
     6421 ccgatggtcg ttgagcaaac gaaccgtggt gaacgtgctt acgatatttt ctcaaggctg
     6481 ctaaaagatc gcatcatttt tattggcagt gctattgatg acacaattgc taatttagtg
     6541 attgctcagc ttttattttt agaatcagag gacccggata aggatattaa tctctacatt
     6601 aattctcctg gtggttctgt ttattcaggt ctggcaattt atgatacgat gcagtatatt
     6661 aaacctgatg tctctacgat ttgcgtaggg atggctgcca gtatgggggc agttttgctg
     6721 accgcaggcg ccgcgggaaa acgttatgcc ttacctaatt cccgtgttat gattcatcaa
     6781 ccacttggtg gtgcgcaagg tcaggctgta gacattgaaa ttcatgcccg tgagatttta
     6841 cgcatcagag aaggattaaa tgaaattttg gctaaacatt ccggcaaacc aatagaacaa
     6901 attactagtg atacggatcg cgacaacttt atgtcagccg aacaagccaa agcatatgga
     6961 ctgatcgatg gcgtactgca aaaacgctag actcctctag gaggtacatt tatgtttaag
     7021 ttcaatgatg aaaagggaca attgaaatgt tccttttgcg gcaagaccca ggaacaggtt
     7081 cgcaaactcg tagcgggtcc cggtgtttac atttgtgatg aatgcatcga actatgcaac
     7141 gagattattg aagaagagct ttctgaggaa atggaactag atttaattga tttgccacgt
     7201 cctactgaaa ttaaagatat tcttgaccaa tatgtaattg ggcaggatga atcaaagaaa
     7261 tcattggcag ttgctgttta taaccactac aagcggatta atgcagaaca aaaacccgat
     7321 gatgtggaaa tacaaaaaag taatattctg ctgattggtc ctactggtgt gggtaaaact
     7381 ttgttagccc aaactctggc tcgtattctc aatgttcctt tcgccattgc tgatgccaca
     7441 tcattaactg aagctggtta tgttggggaa gacgtagaaa atattctgct caaactaata
     7501 caagctgctg actatgattt ggaaaaagca gaaaagggta ttgtctatat tgatgaaatt
     7561 gacaaggtgg cgcgtaagac tgataaccct tctattacca gagatgtctc aggagaaggt
     7621 gtccaacaag cacttttgaa gattttagag ggtacggtgg ctagtgtgcc gccacaaggt
     7681 ggacggaaac atccgcatca agaatttatg cagatagaca ccaccaacat tctctttgtc
     7741 tgcggcggcg cctttgatgg tattgaaaag atcattcaga gcagaattgg caataaaggg
     7801 attggctttg gcgcggaggt ctctggtaaa aaagagaaaa acattgggga tattttacgc
     7861 catgttctgc cgcaagacct gactaaattt ggtttgattc ctgaatttgt cggccgtgtt
     7921 cctgtggtag cgaccctaga tgctttagac aaaaatgctc tcatgcgcat tttaactgaa
     7981 ccgcgcaatg cgctcattaa acaatatcag cgcttgtttg agatggataa tgtggctctg
     8041 gaatttaagg aaggcacttt agaagttatc gcagaagaag cgattaaccg tgaaacaggt
     8101 gcccgagggc tgcgatccat tttagaaagt actttacttg atgtaatgta tgagctgcca
     8161 acattcgagg acattagtaa atgtatcatt actaaagaag ttatcaccaa acaagacaaa
     8221 ccgattctag ttacggttga taaacgtaag aagaaaaaag aagaatcagc ataagcaaaa
     8281 acagtaaaaa actgctagcc ttaaggttag cagttttttt gcctgcagaa acttttgaca
     8341 atcatcataa aggagtacgg ttttttactt tctcttgggg ttatactatc agcagtagtt
     8401 tttaagtacc aaggggggga atatcatgtt acgtttagcg cccatcattg gtgttgtgca
     8461 aattattatt gcgatagtga tcggcatcta tttttggagt ttactgcgct cgcaacgttc
     8521 caacaaagtt gcggtcaagc aggagtcgaa caaagaaatg gatcagttaa gaaagttgcg
     8581 cgagatttca cttaccgaac ctttagccga gaaaaccaga ccagctagct ttgcggaaat
     8641 tgtggggcaa gaagaaggca taaaatctct gcgggcggct ttatgtggct caaatccaca
     8701 acatgtcatt atttatggac cgcctggtgt tggtaagaca gcagctgcgc gcttggtctt
     8761 ggctgaggcg aaaaaaaacg cagcctcacc ctttggtcca gaagctaaat ttgtggaact
     8821 agatgcaacg acagctcgtt ttgatgagcg gggcatcgct gaccccttaa tcggtactgt
     8881 tcatgatcct atctatcagg gcgctggacc tttgggtatg tcaggagtgc cgcagcctaa
     8941 ggcgggtgcg gtgagtaaag ctcacggtgg gatcttattt attgatgaaa ttggtgaatt
     9001 acatcctaca caaatgaata aactgctaaa agtgctagaa gaccggaaag tgtttttgga
     9061 gagctcttac tataatgctg aggataaaaa tataccacta cacattcatg aaatattcca
     9121 aaatggcttg ccggcagatt ttcgcttaat tggcgctaca acacgtacac cagaagaaat
     9181 tcctcccgct attcgttcac gctgtttgga aatctttttc cgtggtcttt ttgcggacga
     9241 aattgctgta gttgcagcca acagtgccaa gcggattaac ttttctattg acgagatggc
     9301 acttgactta atcactaaat atgcgaaaaa tggtcgtgag gcggttaata ctattcaaat
     9361 cgctgctggt attgctattt gtgataaagt gacgcaagag attaccgtag atgatgtgga
     9421 atgggtgatt aatgccagtc agatgtcacc acgtcccgaa attaaaatcc cgcctcgttc
     9481 ccaagtgggg tacgtcaatg ggttagccgt ttatgggcca aacatgggca tggtgctaga
     9541 aatagaagta gcagctcacc aggctgagcc tgggcgaggt caagtacacg tcacaggagt
     9601 ggtggaagaa gaagaattgg gtggcggcca gcgtttaatt cgacgtaaaa gcatggctaa
     9661 aggctctgta gaaaatgtct taactgtgct gcgtgactat ctggaagtcg atgcacagga
     9721 ttatgatatt catgttaatt ttcccggcgg cacaccgatt gatgggcctt cagccggtat
     9781 tgccattgct acggcgattt attcggcact gaccaatatc cctgtgctta acacggtagc
     9841 tatgacggga gaaatatctg tacggggtta tgtgcgtcct atcggcggtg tagtctgtaa
     9901 agtggaggca gcacgccagg ctggcgccaa gaaagtaatt atccccaaag aaaattggca
     9961 aaaaatgttt accaagctgc ctgaggtgga ggtagttccg gtagaaaact tacgtgaagt
    10021 cttggatcat gcactttgcc gatcgcccca atctacacgc aaagcacaaa cacagatgct
    10081 atcggcatca tcagtttagt gcagctaaac taagctatgt ggttaaaggt ggaaagtgga
    10141 ctggttaaaa aggaaatcaa ccaactttag cgaatacgac tactagtgta actatatccg
    10201 aaatggaggc agagcttaat ggctaacgac acaaaacaaa taccgcttct gcccttgcgt
    10261 ggaattctag ttttccctaa tatggttctt cacttggacg taggacgaga acgctcagtg
    10321 aacgccttag aacaggctat gatcgaagat aataaaattc ttttagtagc ccaaaaggaa
    10381 gctagagtcg atgagccaac tcctgacgat atttattcgt tgggaacaat tgctcaagtg
    10441 aagcagatgt taaagctgcc cggtggcact atcagagtgc tggtagaagg attatcacgg
    10501 gcaaaagtac aaacgtttat agatgaagaa ccctatttta aagcagaagc ggtagaactt
    10561 gcagaagatg atacaaaaag tgtagaagta gaagcactga tgcgcagtgt gatctatcag
    10621 tttgaacagt atattaagtt aagcaaaaag attccacctg aaacactggt cactgtatct
    10681 agtgttgaag agccaggtcg aatggcagat ctaatagcct ctcatttaac gctaaaaatc
    10741 caacaaaagc aggagatatt agaggccatt tcaccaacta atcgcttgga gaaactctct
    10801 aacatcctga gtcacgaaat ggaaattctt gaagtagagc gtaaaattaa ccttcgtgta
    10861 cgcaagcaga tggaacgtac acaaaaagaa tactatttac gtgaacaaat gaaggcaatc
    10921 caaaaagaac tgggtgaaaa agatgagcgt ctggccgaag ctgatgaata tcgagaaaaa
    10981 atcagcgaag ctcaattgcc ggacgaagta gaggaaaagg cgcttaaaga agtagaacga
    11041 ttagaaaaaa tgccgccggc tgcagcagaa gctgtggtga tacgtaatta tctggactgg
    11101 atactggctt tgccatggac cactcaaacc gaagatcggc tcgatttagg gatggcggaa
    11161 caaattcttg acgaagacca ctatggcctg gaaaaagtta aagagcggat tattgagtat
    11221 cttgctgtac gtcagctggc gcagaaatta aaaggaccaa ttttgtgcct ggtagggccg
    11281 cctggagtgg gaaaaacctc cctggctcgt tcggttgccc gcgccctgca gaggaatttt
    11341 gtccgtcttt ctttaggcgg cgtccgtgat gaagctgaga ttcgcggtca tcggcgcact
    11401 tatgtgggcg cgatgcctgg tcgtattatc caagccatgc ggcagggcaa atcgcaaaac
    11461 ccagtctttt tattagacga aatagacaaa atgtctacag acttccgtgg tgacccttca
    11521 gccgcactcc tggaggtttt agatccagag caaaataata ctttttctga tcattattta
    11581 gaaattcctt ttgatctttc gcacgtgatg tttattacta cggctaatac tctttataat
    11641 atccctcggc cacttctgga tcggatggaa acaatctatt tgccgggcta tacagaagaa
    11701 gataaactgc aaattgcgaa gcgctatttg ctgcctaaac agattcgcga aaatggcttg
    11761 ggaaaagaaa atatctcaat ttccgagaaa acactacgca aagttatccg ccagtataca
    11821 cgagaagcag gggtcagaaa tttagaaagg caaattgcga ccctctgccg aaaagttgct
    11881 aaggatgtag tgcgcgataa taaacttaaa tcagctatta ctgtgcagaa cttgcataaa
    11941 tacttgggtg tgccgcgctt ccgttatggc atagccgaga caaatgatga agtgggcatc
    12001 gctaccgggc ttgcctggac agaagctggc ggtgatacac tgattattga agttaccctc
    12061 atgcagggta agggcaaatt gttgctgacg ggtaagctgg gcgatgtcat gcgggaatca
    12121 gcacaagccg gcttatctta tattcgttct cgtgcgaagg aattaaatat tgctgaagat
    12181 ttctatgaaa aatatgatat tcatattcat attcctgaag gcgccattcc aaaagatggt
    12241 ccttcagcag gcattaccat ggcgactgct ttaatttccg cgttaacgaa acgaccctac
    12301 aaaagaactg tggccatgac tggtgaaata accctacgcg gccgtgtgct gcccattggt
    12361 ggactgaaag aaaaagtttt agctgcccac cgcgccggca ttaagacgat cattctgcct
    12421 gctgataaca aaaaggatat ttctgaaata ccggaaaatg tacgccgtcg tctggagtta
    12481 atcccagtcg agcatttgga tgaggtatta gagctcgcct tagcggctgt tccagaagaa
    12541 gcagcagagg ttatagagga atgaaaataa gtacggcgga gtttattatt agtgccgtaa
    12601 aaaatacgca atatccacaa acagacctgc cggaagtggc gtttgtcggg cggtctaacg
    12661 ttggtaaatc ttctttatta aactcactgc tcaaccgtaa aaagttagcc ttaaccagtg
    12721 gtagtccagg gaaaacacgt ttgatcaact tctttttgat taatgaagct ttttactttg
    12781 ttgacctgcc aggctacggc tttgcccgtg tctcgcagga gatgaagcgg cagtggggac
    12841 agattattga agcgtattta actagtcgtg aaccacttaa gctggttgtc cagttggtag
    12901 atgtcaggca tccccccaca gcagatgatt tgcagatgta taagtggctc attcacttta
    12961 acttacctac agtaattgtg gcaactaagg ctgataaaat cccccgcggt aagtggcaaa
    13021 agcacctcca gcagattcgg tcgggactgg ggctgggtcc acagggaccg gtaattcttt
    13081 attctgcaca aaccggtcag ggtaaagatg aattgtgggg agttataaaa caatatgtac
    13141 tatcaagggg gtagagcagt ctaccccttt ccctttgctg ctgctgttga tgagacgact
    13201 gcaggaacaa ttttaccctt ttcaccgagc aaaccgaaca gagcaagcct ttcttgacat
    13261 caaatctgcc atctattata gtgataaagg agcaagaatt gagtgtgtgt atgttagtga
    13321 ggaggacttt taatgacaga taaaaatctt gcacctgtat atgacccctc tgaagttgag
    13381 gagcgtttgt atcaggaatg gttggagaag gactattttc aagcaccacg tgatcttaaa
    13441 aggaaaacgt ttaatattgt cattccacca cctaatgtaa caggatcttt acatatggga
    13501 catgctttag ataatactat tcaggatatc ctcatccgcc gcaagcggat ggagggtttt
    13561 gataccttat ggctgcctgg cacggaccat gcaggtatag ctacacagat taaagtggaa
    13621 gagcatttag ccgcagatgg cttaactcgt tacgatttag gtcgtgaaaa atttattgaa
    13681 agagtttggc aatggaaaga tgagtatcat gagcgtatta ttggccaact ttataaacta
    13741 ggtgtctcct gtgactggtc acgggagcgc tttaccatgg atgaaggttg ttcagacgct
    13801 gtgcgtcagg tctttgtggc gctctatcat aaaggtttga tttatcgtgg taattatata
    13861 attaattggt gcccgcgttg tcatactgcc ctttctgata ttgaagtgga gcacgaagat
    13921 gcagaaggga ccttaacgta tattcgctat cccctggtag atggtgaggg acatgtggtg
    13981 gttgctacta cccgtccgga aacaatgttg ggggatactg ctgtggctgt gcatccacag
    14041 gactcacgtt accaggcttt aatcggcaaa gaagtactgc tgcccttaat gaacaggaag
    14101 attcccatta ttgctgatga ctatgttgac ccagaattcg gcagcggcgc tgtgaaaatt
    14161 acgccagctc atgaccccaa tgattttgct atcggtgagc gccatcaact accctcggta
    14221 gtggtgattg cggaagatgg taccatgact caggaggctg gcaaatatgc cggtttagac
    14281 cgtacggaag ccagaaaaca ggtggtagac gatttagaag cggaaggtgt actcctgcaa
    14341 atagaaaagc atcagcatgc tgtgggacac tgccagcgct gccatactgt gattgagccc
    14401 cttttgtcta aacaatggtt tgtcagtatg aaacccttgg cagagccggc aattcagcaa
    14461 gtaaaaaacg gggaaatacg gtttgtgcct gagcgtttca ccaaggttta tttaaactgg
    14521 gtagagaata tccgtgactg gtgtatctca cgtcagattt ggtggggtca tcgtattcct
    14581 gcctggtatt gtgagtgtgg caaaacaatc gtggcacgcg aagaaccgca gacttgcccg
    14641 cactgtggcg gacaggcttt aaaacaagaa gaagatgtcc tggatacttg gttttcttca
    14701 gctctctggc cattttctac tttaggctgg ccggaggaaa cagccgatct agagcactat
    14761 taccccactg atgtcttagt taccggttat gatataattt atttctgggt agcccgcatg
    14821 atttttatgg ggctggaatt tatgcacaag aagccattcc ataccgttta cattcacggt
    14881 ttagtacgtg atgcgcaggg tagaaagatg agcaaatcct tgggtaacgg cattgacccc
    14941 ttagaaatta tagagcagta cggtgcagat acactgcgct ttactttaat taccggtcag
    15001 gctcctggcc atgaccagcg tttccgacag gaaagtgtag ataacagccg caactttgct
    15061 aataaaattt ggaatgcttc gcgttttgtg ttaatgaatc taggcgatac tacggctgca
    15121 gaattagagc tggacagctt aacagatgca gaccgttggc tgctgcatcg ttataaccag
    15181 actgtgcgcg aagtgaaccg cttgatggat acttacgaac taggtgaagc tgcccgtacc
    15241 atttatgagt tcctctggag cgatttctgc gattggtata ttgaactggc taagatcccc
    15301 ctctatgatg cccagacaga agcggaaaaa caaactgtgc gctcagtgct tacctatgtt
    15361 cttgatcgta cgctgcgcct cttgcatccg tttatgcctt ttattaccga ggagatctgg
    15421 caaaaaatcc cgcatagcgg taaaacaatt atgctggcat cctggccaga gtatgaccca
    15481 gccctggagt tttctgcggc ccatgaacgg atggccacag taatggaagt gactcgtact
    15541 atccgttatt tacgtagtga agtgcagctg ccgccaggca gaaaggcgca agtgatactt
    15601 aatgtggaca gtggagcatc ggcacaagca ttagccgctg gggaagctat gttgaagaaa
    15661 ttggcaaatc tggaggaaat gactgtagtg cgggaactgc cggagcagcc ccaacaggca
    15721 cttacggcag tggttggtga tatagagatt tacctgcccc taaaaggcct tgttgatatc
    15781 gaacaggaaa taaaacgctt acaaaaagaa ttgaaaaaat tagaatcaga agtagcacgt
    15841 gcagaaggta agcttaataa tcagggcttt ttggctaaag ctccgcagca ggtgatagca
    15901 gaagaacggg ctaaagccga aaattataga ttacggcgcg acaaggtccg tgagcggatc
    15961 atcagtctgc gctagagcgg aggcaagggt agatgaaata tgaagaagct ttaaactgga
    16021 ttcatggttt gtatcgtttt ggctccaacc taggcttgga gcgagtcaca aagctaatgg
    16081 agctgttgga caatccacag gataagttcc gtagtgtaca tgtagcgggc acaaatggta
    16141 agggttcgac agcagctttc ctagcccaga ttctgcaagc agaaggatta agtgttggtc
    16201 tttacacatc gccatatatc gaagctttta cgaaccgtat ggcgcttaat ggtcatgata
    16261 ttggtgaagc agaattggta gagcttgtaa aacaagtgaa gtgggcagtg gatgaactag
    16321 ctgcctcagc aataggtcag cccacagaat ttgaagtagt tacagcactg gcctttacct
    16381 actttgccca aaatgcacca gactgggtgg tagtggaagt cggtttgggg ggacggctcg
    16441 atgcaacaaa tgtcatcacc cccactgttt cggtcattac caatattgcc ctagatcata
    16501 cagatgtctt aggcggaact attgcggcta ttgctggtga aaaagcagga attatcaaac
    16561 ctggtgttcc tgtggtcacg ggagcacaaa aggatgaggc cttagcagtt ttccagcagg
    16621 ttgcggctga gcggcagtcc ccgctttacg caatagatca tgattttacc gaggaagtga
    16681 cggcatcttc cttggcgggg ctgactttta gctaccaaag cccttggcat cagctgtctg
    16741 atctacaggt ccagctatta ggacgccatc aagcgcgcaa tgcttcagta gcgatcgctg
    16801 ccagagagtt actgcccctg ccctttagcg aagaagctgt gcgcgagggg ttaaggaaaa
    16861 caatgtggcc aggtcgcttg gaggtattct cacaacagcc tttagtcatg gttgatggtg
    16921 cgcataatcc tgacggtgtt ttagccctgc gttcagccct gctggaactg ctgggggaca
    16981 gaaaactacg tctggtgatt ggtatcctgg gggataaggc cgttgaggag attgttggtg
    17041 tcattgcccc cttggctcaa gctgggatta ttgttaccac accgaataac ccccgtgccg
    17101 ctgaccccta tgttgtggca gaactggtcc ggaagttcgc aggcaacacc cctgttgagg
    17161 tggtaccaga aattcaggct gcggtgcagc aggctgtagc aataacgcag cccaatgagg
    17221 ccttatgtgt ttctggttcc ttatatacaa tcagtgaagc acgtgaggtt ctaaaaaata
    17281 tttat
//
LOCUS       DASRMS010000009         1675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_69029, whole genome shotgun sequence.
ACCESSION   DASRMS010000009 DASRMS010000000
VERSION     DASRMS010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1675
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69029"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(172..441)
                     /locus_tag="VFC74_00425"
     CDS             complement(172..441)
                     /locus_tag="VFC74_00425"
                     /inference="COORDINATES: protein motif:HMM:NF027408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HZK23833.1"
                     /translation="MPQRKIMRIRDYVNQIPDAAAISTAQTTTISSAAVGFLSWVGSI
                     NWVGLISVLVAVAGLIANVYFSYRNHKREEELHQFTLKIKGACDE"
     gene            complement(492..725)
                     /locus_tag="VFC74_00430"
     CDS             complement(492..725)
                     /locus_tag="VFC74_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23834.1"
                     /translation="MTTEIETDIQPRAEWSREEVERRRRKAYADPINGSDRHFNEAAR
                     LRASGEREEAEIAEQKGIEAALKIKDKYPYPEN"
     gene            complement(1025..1363)
                     /gene="avd"
                     /locus_tag="VFC74_00435"
     CDS             complement(1025..1363)
                     /gene="avd"
                     /locus_tag="VFC74_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diversity-generating retroelement protein Avd"
                     /protein_id="HZK23835.1"
                     /translation="MKSNTEPKPLLIKLKIEQMIIWSNSPLKNFPRHEKYVMGAEIRK
                     SMYQLLRLVIKANRQHHRKTTLLEVDVELDFLRSLVAEASDPSKRYISRNQYKTWSEH
                     LAVIGRLVGG"
     gene            complement(1400..>1675)
                     /locus_tag="VFC74_00440"
     CDS             complement(1400..>1675)
                     /locus_tag="VFC74_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23836.1"
                     /translation="IDTLVEAAGNGTFSTRPYDDPPLILKQAMAVPTAGLTHDGYFWK
                     NETGQRVPRRGGHWSSAGDAGLDALALNNTRTRTDTYIGLRPRFRNQ"
BASE COUNT          382 a          431 c          340 g          522 t
ORIGIN      
        1 gggcttgtcc acttggtaat acctttgcat accgtgggca gtccaccagc taacttatca
       61 gcataaacaa catactcgcc ctcgccctcc catttaccta agaacgacaa caaaaaaggt
      121 gatgctaaac taatcgcacc gcctatcact aaagctatta tcccgttacg cctattcatc
      181 acacgcgcct tttatcttta aagtaaactg atgcagctcc tcctcgcgct tatgattgcg
      241 gtacgaaaaa taaacattgg ctatcagtcc agcaacggcc accagcacac taattaagcc
      301 aacccagtta atgctgccca cccagcttaa aaaccccacc gcagcagagc tgatagtggt
      361 cgtttgagcc gtggaaattg cagccgcgtc cggtatttga ttcacgtagt cacgtatacg
      421 catgattttc ctttgaggca tagctgcctc cctatttttt gacaataaaa aaaccgctta
      481 aaaagcggta tttagttttc tggatacgga tatttatctt ttattttcaa agccgcttcg
      541 atgccttttt gctctgcaat ttctgcttct tcgcgctctc ctgatgccct tagcctcgcg
      601 gcttcgttaa agtgcctgtc gctgccgttg attggatctg cgtatgcctt tctgcgcctg
      661 cgttcgactt cctctctgct ccattcggcg cgcggctgga tgtctgtttc tatttctgtc
      721 gtcattttca atgctcctaa tgaatggatg tttgtttaaa tatttttcaa ttgctgctga
      781 tgcatcgcca tgtcttgcgt gtgctatcca gctagctagt tgctgaccaa tttcttttgc
      841 tgtgatttct ctatttgcat aaagtctttg aagtctcctt actctacgtt taaacctgct
      901 caagctggct tttcgtaacc tcctcttgtg cggccataag tgataaccaa gaaaatctaa
      961 tcccctgcca ttttttactg ccactggaaa cacttgcgtt ttatgattaa gctctagttg
     1021 caggttatcc gccaaccaat ctaccgatga cggccagatg ttctgaccat gttttatatt
     1081 gatttcttga gatgtatctt ttacttggat cacttgcttc agcaaccagc gaacgtaaaa
     1141 aatccaactc aacatcaact tcaagcaatg tggtctttct gtgatgttgt cgatttgctt
     1201 taatgactaa tcgtaaaagc tgatacatgc tctttctgat ttctgcgccc atcacgtatt
     1261 tctcatgacg aggaaaattt ttaagcggag aatttgacca aataatcatc tgctctattt
     1321 ttaacttgat tagcagaggt ttaggttctg tgtttgattt catgttttta acgctcagct
     1381 gccgctgagc tctagcagat tactgattgc gaaagcgggg ccgcaacccg atgtacgtat
     1441 ccgtgcgcgt ccgcgtgttg ttcagagcca acgcgtcgag gcctgcgtcg ccggcactgc
     1501 tccaatggcc gccacgcctc gggacccgct ggccagtttc gtttttccaa aaataaccgt
     1561 catgagttag gcctgctgtt ggcacggcca tcgcttgctt cagaataagc ggcggatcat
     1621 cgtagggtct cgttgagaat gtgccattgc cggctgcctc aacgagcgta tctat
//
LOCUS       DASRMS010000010        41407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_1655, whole genome shotgun sequence.
ACCESSION   DASRMS010000010 DASRMS010000000
VERSION     DASRMS010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 41407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41407
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1655"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1049)
                     /locus_tag="VFC74_00445"
     CDS             complement(45..1049)
                     /locus_tag="VFC74_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK23837.1"
                     /translation="MRYRVSAILKSPKVLQLFFMLLLLLIIANYFRPVLSPATSICAG
                     VNLAGKDLGGLTTEEAKAVIEQVARMQDTPPIDCRIDPTTNGVIPGLNGLSIDVAETL
                     ARVSSAQEHETVKPVFRQAAPAVGLTAFPEHPIYQGNPVKQQVAFLINVAWGNEYLPE
                     MLAVLKEQQAEAVFFLVGRWVQANEADARLIADAGFELGNHGYSDALSMQELTLAAAT
                     DDISRAAEVIEKVCGKRPVYFSPHRGELSADVLKAAASLKQRTVMWTVDTVDWMLPGV
                     SVMLDKILSKAEGGSLILMHPTAQTPEFLRQVIPALRTKGLEPVSLTKLLNPMRAAKE
                     "
     gene            complement(1063..1677)
                     /locus_tag="VFC74_00450"
     CDS             complement(1063..1677)
                     /locus_tag="VFC74_00450"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK23838.1"
                     /translation="MPLVKGAYYQVETQDRAVALTFEVVWEPGFTHEVLNILDRYQIR
                     ATFFLTGSWLRQNPDLAREIIDRGHEIGQHSYAHKHLTDLDDEELTKEFDLMAETLQE
                     ELNLQTHLFRPPYGELDQRVFEFAAKRGYTTVVWSINPHDWQEPGKDKIISRITKQLH
                     NGAIILMHTNSSQAVEALPVILQSLLMQEYEVLPFSELAQRGLK"
     gene            complement(1926..2744)
                     /locus_tag="VFC74_00455"
     CDS             complement(1926..2744)
                     /locus_tag="VFC74_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK23839.1"
                     /translation="MLSNDLTTQAASAEHLAYLQARKRYRWRVTAAQILLLVSFIALW
                     ELAATLRWIDPFIMSQPSRIVQTLRSLYAGGDLFTHVGVTLLETVAGFTLGTLLGTFI
                     AVALWWSDYLAEVFDPYLVVLNSLPKVALGPIMIVWLGQGMRAIIAMALLVSVIVTIL
                     SVYSGFSQVDKNKIKLLKTFGATKKQIMQKVILPASIPTIISALKINVGLSWVGVIVG
                     EFLVSRAGLGYLIVYGGQVFRLDLVMASVIILCVAAALMYQAVVLLEKRFVKWQ"
     gene            complement(2731..3510)
                     /locus_tag="VFC74_00460"
     CDS             complement(2731..3510)
                     /locus_tag="VFC74_00460"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK23840.1"
                     /translation="MGAEKLLRIEKVSMKYYSPEGEISALQDISLDIAEGEFVSIVGP
                     SGCGKTTLLNILAGLITPSAGAVYIQEQLVSGATPLVGYMPQDDQLFEWRTIWHNVLL
                     GLEVQNNVNQATKAAAEKMLRTYGLIEFKDAYPSQLSGGMRQRAALIRTLAINPEILL
                     LDEAFSALDYQTRLVVADEVKEIISSENKTAVLVTHDIAEAISMSERVVVLSKRPGTI
                     KNIHDIVLTCEINSPLGRREAIEFRAYFNRIWKELDVHVEQ"
     gene            complement(3531..4553)
                     /locus_tag="VFC74_00465"
     CDS             complement(3531..4553)
                     /locus_tag="VFC74_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020457868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK23841.1"
                     /translation="MKGVSIVQKRVVVYVLVPLLLVALALTGCERDDSLTKVRLSEVT
                     RSVFYSPLYAAINLGYFEDEGLELEVTTGQGADKVMTALLSGEVDVGFMGPEASVYVH
                     NQGQDNYAVNFAQLTTCDGSFLVAREPDPNFTWDKVRGKSIIGGRKGGMPLMTLEYVL
                     KNQGLMPGEDVEVMTHIQFALMGGAFTGGEGDYVTLFEPVASALEMEGVGYVVASVGA
                     DSGKIPYTVFCARKKLLAENPEIIQKFTNAIYRGQVWVQESTPEEIAQVIAPSFPDSD
                     EVVLAKVAARYKETDAWAATPVFTEEAFARLQDVMETAGELDKRAPYSDLVTNEFAEK
                     AMELVR"
     gene            4677..4982
                     /locus_tag="VFC74_00470"
     CDS             4677..4982
                     /locus_tag="VFC74_00470"
                     /inference="COORDINATES: protein motif:HMM:NF013957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HZK23842.1"
                     /translation="MSNAMPSVLVHAEKNTYKLGDSVIITIIAKAEGQPAPHVPLKLY
                     VFDPTGKKVFQSSLHTNINGKAMANYQLKKTLPTGNYYLEAQNKNRSVALSSFIVLQ"
     gene            complement(5062..7248)
                     /locus_tag="VFC74_00475"
     CDS             complement(5062..7248)
                     /locus_tag="VFC74_00475"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014261973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HZK23843.1"
                     /translation="MCMKVYKMQLAGRELSFETGRIAQQASGAVLIRYGDTVLLCTAT
                     ASAEPREGIDFFPLTVDYEERLYAVGKIPGGFIKREGRPTEKAILAARLTDRPCRPLF
                     PKGFRNAVHIVSTVLSVDQDCPPEVLSICGASAALSISHIPFGGPIGAVVVGWVDGKP
                     VINPTQAEAEASRLHLTVASTKDAIMMVEAGAHELTEAEILEAIMAGHEANRTIVALQ
                     EQMMAELGQPKMAVSQFKPAEEIAAEVRQFSQEKLYNAVRTIEKQARETAIDELRNET
                     VTHFAEKYPDNERDVRVAYENLIKETMRTMIIKENVRPDGRKSDEIRPISVEVGILPR
                     THGSGLFRRGQTQVMNICTLGAPGDVQRLDGLGLEESKRYMHHYNFPPYSVGEAGFMR
                     GASRRDIGHGALAERALMPVLPTQEEFPYTMRLVSEVVESNGSSSMGSVCGSTLSLMD
                     AGVPIKKPVSGIAMGLIIEGDDAAILTDIQGMEDFLGDMDFKVAGTKDGITALQMDIK
                     IKGLSRELLTRALAQAKTGYMYIMDQMTAVIPEPHAELSPYAPRIITMQINPDKIRDV
                     IGPGGKMINKIVAETDAQIEIEADGTIYIAAVDSAGGLRAQQMIENLTKDVEIGTIYQ
                     GKVTRVEKYGAFAEVLPGKEGLIHISRLAHERVEKTEDVVNLGDEIPVKVIGIDDRGR
                     IDLSRRAAIPNAQGEMIEEEPQQRRDDRRDRRPNNNGRDNKRSRRT"
     gene            complement(7413..7676)
                     /gene="rpsO"
                     /locus_tag="VFC74_00480"
     CDS             complement(7413..7676)
                     /gene="rpsO"
                     /locus_tag="VFC74_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003375280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HZK23844.1"
                     /translation="MDTARKQDIINSFRLHEHDTGSPEVQIALLTERINYLTEHLKEH
                     KKDHHSRRGLLKMVGQRRGLLNYLKKIDAERYRTILTRLSLRK"
     gene            complement(7827..8774)
                     /locus_tag="VFC74_00485"
     CDS             complement(7827..8774)
                     /locus_tag="VFC74_00485"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HZK23845.1"
                     /translation="MEIVHGIESFQLCHREQVYLALGNFDGVHCGHKAIISTTVALAR
                     EHHKKSAVLIFFPHPLSILKPGKSTKLLNTAADRIRMLGEAGIDYVIIHPFTPDFAAI
                     SPQQFVVDILHRQLSVAGVIVGYDYSFGSRGIGTPADLQRLAQDLGFSVQVVERISIL
                     DQPVSSTAIRQLLQQGKMEEANLMLGYPYFLRGTVIHGDGRGRTLGFPTANLQLPAEV
                     ILPANGVYLTQVKLKEVKYWALTNVGMRPTFKKDAVSVEVYLFDTEKNLYDQELFVSF
                     LQKIREEMSFTDVAALVAQIQADVRQAKNLICDLPKQEQ"
     gene            complement(8780..9661)
                     /gene="truB"
                     /locus_tag="VFC74_00490"
     CDS             complement(8780..9661)
                     /gene="truB"
                     /locus_tag="VFC74_00490"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HZK23846.1"
                     /translation="MNGIINLLKPPGMTSHHVVSALRRLTGIRKIGHTGTLDPGAAGV
                     LPICIGQATRVAEYVLTMDKTYRAELTLGTATDTEDAAGQVIDEKTVPPLDFNQVKTL
                     LLDFTGPRQQIPPMYSAVRVHGQKLYQLARRGETVTRKARAINIYQLQLLQLYPQKIL
                     FDVSCSRGTYIRTLCREIAEALDTVGHMSFLVRTRVGPYHLDQTYTLEECASLQAANE
                     LTTALLPLDSALEQLLPYNVTAEDAEKIQNGRVLKFTDLPPEGTLMRVYGPGGELLAI
                     SSVENSTVRPQKVFRSR"
     gene            complement(9658..10617)
                     /locus_tag="VFC74_00495"
     CDS             complement(9658..10617)
                     /locus_tag="VFC74_00495"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HZK23847.1"
                     /translation="MHSLAEIAQKLKTCRAVLITAHIEPDGDAIGSVLAMGLALQQLD
                     VAALMFSADDVPKRYQFLVGTNRFYTHRLPTTSFDCVLTLDCADSKRLQPIWGEIQDK
                     QIINIDHHPTNELFGHLNYVDHTAAATGELVFALLQELGCQIDLEIAKALYVAISTDT
                     GSFKFESTTARTHTMAAELIAAGVNPGALSPLIFDLQTPAALAILRQALSSLTLSADG
                     KIAWIVLTDADMKEAQAHDEDLGGVINYAKNIVGVEVGLIFREQADGTIKVGFRSHQI
                     DVAKVAASFGGGGHVRASGCSLAEDLPTALQLVLASVSEEINA"
     gene            complement(10610..10969)
                     /gene="rbfA"
                     /locus_tag="VFC74_00500"
     CDS             complement(10610..10969)
                     /gene="rbfA"
                     /locus_tag="VFC74_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011460392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HZK23848.1"
                     /translation="MSEQRAHRVAEEIKREMSSILRDEMKDPRVSGMISVTSVEVTRD
                     LSYAKIYLSVFGNDEEQQDTLTALDKATGFIRSIIGKRIRLRHTPELTFHLDQSIAYG
                     AHISEVLRKLKKDDDGA"
     gene            complement(10996..13299)
                     /gene="infB"
                     /locus_tag="VFC74_00505"
     CDS             complement(10996..13299)
                     /gene="infB"
                     /locus_tag="VFC74_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HZK23849.1"
                     /translation="MTKIRVYQLAKDLDVPSRRIIDLLTEIGCEVKNHMSVVDKEAAQ
                     RITYQLTGKGEPPAAYAKELNKKVEKEQKKETSPNNKSKTTQHDTKKKPQQQSGAATK
                     PKRNSGRSEQSQQASPKQPTASTADQTQKSKPTQGQGSRPVRQSSTGQQRYNAKRQSP
                     KKKIDKRERRARREARLLDEQQKTENTVVLEGRITVGELADKLAVSASEIISKLIGFG
                     VMAAINQAIDTEVAKLLAEEYGFAVEIKVDKFEAELVEAVEEHNEEDLQPRSPVVTVL
                     GHVDHGKTSVLDVIRKANVTAQEAGGITQHIGAYQANYQGKKIVFLDTPGHAAFTSMR
                     ARGAQITDIAVIVVAADDGVMPQTVEAINHVKAAGVPIIIAINKIDKEAANAGRVKQQ
                     LTEFGLVAEEWGGDTIMVEVSALKKVGFDELMEMILLVAEMAEFKANPSKLALGAVVE
                     AKLDKGRGPVATVLIQDGTLRIGDSIICGTIYGKVRAMVDDKGKRIKKAGPSTPVEIL
                     GLTDVPQAGDDLQAVTDDKMARQIAEKRADKKREVELNKSAKISLDDLFNQIQQGEVK
                     DLNIIIKADVQGSLEALRDSLLKLSTDEVRVQVIHGGVGAVAESDVMLASASNALIIG
                     FNVRPEPNARKMAEKENVEIRLYRVIYEAIAEVQAALKGMLAPQFKEVILGQAEVRQL
                     FKVSRLGTIAGAYVLEGKITRSADIRVIRDGVVIHEGKMETLKRFKDDVREVLAGYEC
                     GVLMEKFHDYKEGDIIEAYTIQEIKPA"
     gene            complement(13335..13640)
                     /locus_tag="VFC74_00510"
     CDS             complement(13335..13640)
                     /locus_tag="VFC74_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HZK23850.1"
                     /translation="MPNKALALLGLARRAGKLTWFEEANLAAIRSGQAKLLLLASDAG
                     EAAKKRYRDKSKSYSVPMIVFVDKIQLGAALGTSPRAAVTVTDEGFAERLKTLLIIE"
     gene            complement(13642..13920)
                     /locus_tag="VFC74_00515"
     CDS             complement(13642..13920)
                     /locus_tag="VFC74_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005807691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HZK23851.1"
                     /translation="MSWLKQRKIPLRTCIGCQEQKPKKELVRIVRTPEGRVELDPTGK
                     KAGRGTYLCKQKSCLEKAVKAKRIERNLQQAVSPAVVEELAAQLSEDE"
     gene            complement(13898..14992)
                     /gene="nusA"
                     /locus_tag="VFC74_00520"
     CDS             complement(13898..14992)
                     /gene="nusA"
                     /locus_tag="VFC74_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HZK23852.1"
                     /translation="MNEEFLAALAAIEREKGVKKEILFEAIEAALVSAYKRNFNAAGN
                     VRVQLNRETGGIVVYTRLTVVPEAAQPQLEISLQEARELDPHYNIDDIVEREVTPKNF
                     GRIAAQTAKQVVIQRIREAEREMVFDEYVDREEDIITGIVQRIEQKNIIVDLGKTEAV
                     LLPTEQISSDDYQPGERLKAYITEVRRTTKGPQIFISRTHPGLLKRLFELEVPEIFDG
                     TVEIKSVAREAGHRSKLAVYAKNTDVDPVGACVGPKGVRVQAIVNELRGEKIDIVKWD
                     EEPQLFVANSLSPAKVVSVTLHKDQKVANVIVPDYQLSLAIGKEGQNARLAAKLTGWK
                     IDIASESQAAEQHEAANSSPDEGDELVETT"
     gene            complement(15023..15481)
                     /gene="rimP"
                     /locus_tag="VFC74_00525"
     CDS             complement(15023..15481)
                     /gene="rimP"
                     /locus_tag="VFC74_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HZK23853.1"
                     /translation="MSKITDNLFAVAEPLAANLQLELVDVEYVKEGGQFVLRFLIDKE
                     GGVTLEDCEAFSRIMDSELDQLDPIESSYQLQVSSPGIERPLKKAADYERFSGRLATV
                     KLFAPLNGSKQYTGVLRGLANQEILLAKNKDATVVKIPLELVAKANLVFE"
     gene            complement(15863..16924)
                     /gene="ispG"
                     /locus_tag="VFC74_00530"
                     /gene_synonym="gcpE"
     CDS             complement(15863..16924)
                     /gene="ispG"
                     /locus_tag="VFC74_00530"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HZK23854.1"
                     /translation="MITRENTKAVQIGSLTMGGGAPVVIQSMTNTDTADLEGTLLQIK
                     RLAQAGCELVRVAVPNEAAAAVLPQIVELSPLPVVADIHFDYRLALAALDAGVPKLRI
                     NPGNIGNHQRLQAVVERAAEKNAVLRIGVNAGSLEKRLHEKYGAVIAAALVESALDHI
                     RMVEELGFDNLVISLKASSVPLTVAAHRLLAREVAYPQHVGITEAGSSFSGTIYSAVG
                     IGSLLLDGLGDTLRVSLTADPVEEIRVAKEILAAANLRRFGPRVISCPTCGRTQIKLQ
                     ELARQIEERVKDIKEPLTLAVMGCAVNGPGEAREADLGVAGGKEEGLIFRRGQVVKKV
                     PQDKIVDEFMLALQEYLQG"
     gene            complement(16935..17960)
                     /gene="rseP"
                     /locus_tag="VFC74_00535"
     CDS             complement(16935..17960)
                     /gene="rseP"
                     /locus_tag="VFC74_00535"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HZK23855.1"
                     /translation="MLTLVASVIIFGLLVFFHELGHFLVAKRVGIGVMEFALGFGPRL
                     VHKEVGETVYSIRAFPLGGFVRLVGEEPEEVEAEGSFQKQAVWKRFLVIAAGPLMNFI
                     LAIVLFSLIYFAFLGVPVYSSTAIGELLPEGAAAQVGLQSGDRIITIAGEDVNNWDEL
                     VALIHSHPGKELEIVYERNNQTSTVTVTPERDAETGEGRIGIGAETRKYALLPAIRMG
                     IEHTVWFISFTATSLLQMVTGKVSADVMGPVGIISLVGEVARTGIMNLLSLAAIISLN
                     LGFINLLPIPALDGSRLMFIALEGVRGRPVDPKKESFIHFIGFALLIVLMIVIAYKDL
                     VRLNIFN"
     gene            complement(17965..19122)
                     /locus_tag="VFC74_00540"
     CDS             complement(17965..19122)
                     /locus_tag="VFC74_00540"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HZK23856.1"
                     /translation="MKKISILGSTGSIGKQALAVVEQYPALFCLHGLSAHRQVKELAD
                     QARLYKPAKVVIAEPSLYQELKLACSDLAVEVAAGPEALCDLAADAQHELVLNSVVGF
                     AGLQPTLTALAAGKNVALANKESLVAGGHLVMPLAAAVNKAILPVDSEHSAIWQCLQG
                     QPRAAVEELIVTASGGPFFDFSTAELEHVTPQAALKHPNWSMGAKITIDSATLMNKGL
                     EVIEAHWLFDITYDKIKVVVQRESIIHSMVTFHDGAVLAQLGRPDMRVPIQYALTYPK
                     RLSGDAPRVQWEQLPLLHFAPPDTEKFPCLQFAYEAGKIGGTLPAVMNAANEEAVRLF
                     LAGSISFLAIPRIIESVMNKHKVIMAPEFDVLVAVDQEARKLAQLLGKRKG"
     gene            complement(19122..19916)
                     /locus_tag="VFC74_00545"
     CDS             complement(19122..19916)
                     /locus_tag="VFC74_00545"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HZK23857.1"
                     /translation="MFRQRLLSALVGIPLVVLLIWQGGVAIFVAIIFISSLALREFYT
                     LCSLENRLVRTLGYAGNILLLTVAGLANPEAYFFALIVFFIVLNLVAVFAYPQDYQEL
                     MILLWGALYITSLLAFFLWLRATANGFLLLLSVLVTTWASDTGAYACGLTLGRHKLIP
                     AVSPKKSLEGAVGGLLFAAAAMSLVFAPLLNLNPLTALLLGVAISFVGQCGDLAESAL
                     KRRGKIKDSGKFLPGHGGVLDRLDSLLFAVPVGYYLLLALAAKGGI"
     gene            complement(19916..20680)
                     /locus_tag="VFC74_00550"
     CDS             complement(19916..20680)
                     /locus_tag="VFC74_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HZK23858.1"
                     /translation="MSFSLKRLFQKKIPQPNTSDEIPRHVAIIMDGNGRWAAKRGLPR
                     TAGHHAGMVALRRIIRAANEIGLGVLTVYAFSTENWHRPPAEVNFLMRLPSEFLRTDL
                     PELIAKNVRVMMSGAREGLPDYTLAAIDQAMTSTAANTGMILNFALNYGSRAEILHAV
                     QGIAQAVIAGSLHVQEITEETIKQHLYTSELPDPDLLIRPSGEVRLSNFLLWQLAYAE
                     LWFVDIYWPDFKAEHLLAAIEAYRQRDRRFGGLGGI"
     gene            complement(20716..20934)
                     /locus_tag="VFC74_00555"
     CDS             complement(20716..20934)
                     /locus_tag="VFC74_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23859.1"
                     /translation="MHKRTGIPDLLAYPLAEAVRITESWGLTPSLQHTSPPKKINQGS
                     ERVVRVRLTQDKTVLELTVAAEDCGPLV"
     gene            complement(20936..21493)
                     /gene="frr"
                     /locus_tag="VFC74_00560"
     CDS             complement(20936..21493)
                     /gene="frr"
                     /locus_tag="VFC74_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HZK23860.1"
                     /translation="MLNEVYRDAEERMKKAVQALKKDLSTLRTGRATPALLDRIVVEY
                     YGAQTPINQLANISVPEPRLLVIQPWDKKVIGEVERALLKSDLGLMPNSDGSLIRLSI
                     PQLTEERRKELVKISKKKAEDGRVAIRNIRRDANDTTKNMEKAHEITEDELHKAQDEI
                     QKLTDKMIEAADNVLADKEKEIMEV"
     gene            complement(21496..22218)
                     /gene="pyrH"
                     /locus_tag="VFC74_00565"
     CDS             complement(21496..22218)
                     /gene="pyrH"
                     /locus_tag="VFC74_00565"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HZK23861.1"
                     /translation="MEKAVYKRIVLKLSGEALAGERGFGIDSDVLSEIAAQVEEVWKM
                     GVEVAVVVGGGNIWRGAPAGERGMDRATADYMGMLATVLNALALQDALESRGVDTRVQ
                     TAIEMQQVAEPYIRRRAIRHLEKNRVVIFAGGTGNPYFSTDTTAALRAAEIEAEVILL
                     AKGKVDAVYDADPLTNPTAKKFTELTYIDLLNRGLGVMDSTAASLCMDNKIPLIVFGL
                     NKTGNIKRVLLGVKIGTFVKGE"
     gene            complement(22331..22978)
                     /gene="tsf"
                     /locus_tag="VFC74_00570"
     CDS             complement(22331..22978)
                     /gene="tsf"
                     /locus_tag="VFC74_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HZK23862.1"
                     /translation="MIPASVVKELREKTGAGMMDCKKALTEAGGDLEKAAEFLREKGL
                     SAAAKKAGRIAAEGLVDSYIHMGGKIGVLLEVNCETDFVARTDEYRNFVRDVAMQIAA
                     TNPQYLAREDVPAEILDKEREILRAQAKNEGKPEKIIEKIVEGRISKFYTENCLLEQA
                     FIRDTDVTITGLLQDKIAKIGENISIRRFVRYEMGEGLEKKGCNLADEVAAMTKS"
     gene            complement(23150..23899)
                     /gene="rpsB"
                     /locus_tag="VFC74_00575"
     CDS             complement(23150..23899)
                     /gene="rpsB"
                     /locus_tag="VFC74_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HZK23863.1"
                     /translation="MPVVSMKQLLEAGVHFGHQTRRWNPKMKPYIFTERNGIYIIDLQ
                     QTVRMLDDIYRFVRDLALDGRRIMFVGTKKQAQESIQLEAERCGMFYVNQRWLGGMLT
                     NFKTIRKRINRLIELEKMEEDGIFGVLPKKEVIQLRHEHDKLNKFLSGVRNMHTLPDA
                     LFIVDPRKERIAVAEARKLGIPIIAIVDTNCDPDEIDYIIPGNDDAIRAVKLISGRMA
                     DAVLEGLEGRQDHVVVEEQLEVEDNTPAVEL"
     gene            complement(24061..24594)
                     /locus_tag="VFC74_00580"
     CDS             complement(24061..24594)
                     /locus_tag="VFC74_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23864.1"
                     /translation="MGRSKYRWVLDRKSYLFGVGSALAVVVVFSAMALYYVAAYGVTV
                     HLDSEALARTIELEVVSQAKQELPKIITDAKAEIPHIVETEMHDQFSERMEIAGFIFS
                     MPEELMTQLREKMRTNVENATGQILDGINTQILAEQFGDNVYTMVKQTMSGELSAKGF
                     PVYIFDKIPVVVRVQVK"
     gene            complement(24775..25530)
                     /gene="codY"
                     /locus_tag="VFC74_00585"
     CDS             complement(24775..25530)
                     /gene="codY"
                     /locus_tag="VFC74_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HZK23865.1"
                     /translation="MEALLEKSRRINKILQKTAGQHVDFKDVAEVLKNVIAANVYIVD
                     RSGKILGISAIDEFNGEMLSEGDTLPADYNGQLLSYDESKVNLQHDETDALFKDNTTT
                     IIPILGGGKRLGTLLLARNGKDFRSEDLVLAEYGATVIGMEVLRIRADKIEEEARNKA
                     VVQMAIGTLSYSELEAVENIFAELNGDEGILVASKIADQLGITRSVIVNALRKFESAG
                     VIQSRSLGMKGTYIKVKNPYLLEQIEKRNAVAM"
     gene            complement(25562..26983)
                     /gene="hslU"
                     /locus_tag="VFC74_00590"
     CDS             complement(25562..26983)
                     /gene="hslU"
                     /locus_tag="VFC74_00590"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HZK23866.1"
                     /translation="MEKQLNREELTPRQVVAELDKYIVGQKEAKKCVAVALRNRYRRK
                     LLAADFREEVFPKNIIMIGPTGVGKTEIARRLAKLVNAPFVKVEATKFTEVGYVGRDV
                     ETIVRDLVETSVRIIQAEKLGAVEEKAQQLAEERLLELLLPQPPKEKKVQNPLTLLFS
                     GPEEQEENEEDPHYSQSEQQVRTEKRTILAQRLHAGSLENELIEMEVEASPPMMEIIP
                     GSGMEDMGINFQDIMGQFLPKRRKTRKITVAQARKILQQEEAEKLIEMDEVHAEAIER
                     AEQSGIVFLDEIDKIAGKDLKGGPDVSREGVQRDILPIVEGSTVITKYGPVKTDYVLF
                     IAAGAFHVAKPSDLIPELQGRFPIRVELDSLTQEDFYRILTEPANALIKQYTELLATE
                     GVKIIFTDDAIGEIAKTACLLNQEMENIGARRLHTILEKVLEDLSFAAPEIAGQEVTI
                     TAQYIRDKIQQIVQNKDLSRYIL"
     gene            complement(26988..27518)
                     /gene="hslV"
                     /locus_tag="VFC74_00595"
     CDS             complement(26988..27518)
                     /gene="hslV"
                     /locus_tag="VFC74_00595"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HZK23867.1"
                     /translation="MFRATTIVAVIKDGRCALAGDGQVTFGDKTIMKHQAVKVRRIYQ
                     GKVLAGFAGSVADAFTLFERYEEQLEKYQGNLRRAAIELAKDWRTDRVMRRLEALLIV
                     GDLTGILVISGNGEIIEPDDGVTAIGSGSPYALAAARALSRYTKMDAGEIARRALEIA
                     GEICIYTNDRIVLEVL"
     gene            complement(27529..28425)
                     /gene="xerC"
                     /locus_tag="VFC74_00600"
     CDS             complement(27529..28425)
                     /gene="xerC"
                     /locus_tag="VFC74_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HZK23868.1"
                     /translation="MIHWIDGFLSYLQVEKNASYHTIRNYAEDLAQFASFLEKEAIEI
                     SSIDYMTLRQYLALLQKKAYERRTIARKLSAIRSFLRYLNREELLEDKTWHSVSTPRV
                     GKKLPIFLYPEEMIRLLDAPDAKTPAGQRDAALLEVMYASGVRVGELVALNVQTMDLH
                     LGQFIVKGKGARERMVPLGRCACNAVQRYLHEARPQLLRKNSAGEREQALWLNKYGTR
                     LSDRGVRRIVDKYVQQASLTHGISPHSIRHSFATHLLNAGADLRAVQELLGHINLSTT
                     QIYTHLTRDRLKEVYHGAHPRA"
     gene            complement(28516..29838)
                     /gene="trmFO"
                     /locus_tag="VFC74_00605"
     CDS             complement(28516..29838)
                     /gene="trmFO"
                     /locus_tag="VFC74_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HZK23869.1"
                     /translation="MAKITIVGGGLAGAEAAWQAAQSGCEVLLLEMRPQKMTPAHHTA
                     ALAELVCSNSFRGASLENAVGLLKEEMRRQNSLIMAQADKYAVPAGGALAVDREAFAQ
                     GVTEAITNHPLITCREQEVTEIPETTNGPVIIATGPLTSETLAQKIGELTGQSSLHFY
                     DAAAPILTAESIDMERVFKASRYDKGTADYLNCPLGKEEYKSFYEALTTAEVYEGHDS
                     VEEKKYFEGCMPVEVMAVRGFETLLYGPMKPVGLKDPHTGKQPYAVVQLRQDNAAASL
                     YNIVGFQTRLRWPEQKRVFGMISGLEKAEFVRYGVMHRNTYINAPQVLKATLQFAGNP
                     QLFFAGQMTGVEGYVESAAMGLIAGLNAARQLAAKEPQIWPAASAHGALTHYITTADP
                     NNFQPMNVNFGLLPPLAQRIPKKKRKLALAERALTELEAWQKTTLTNY"
     gene            complement(29831..31957)
                     /gene="topA"
                     /locus_tag="VFC74_00610"
     CDS             complement(29831..31957)
                     /gene="topA"
                     /locus_tag="VFC74_00610"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HZK23870.1"
                     /translation="MSDYLVVVESPTKAKAIKRYLGSRYKVIASLGHVIDLPKSQIGI
                     DIENNFTPKYITIRGKGKVLKELKNASKDAKKVFLAADPDREGEAICWHLARALKLQQ
                     DIPCRVEFHEITKKAVKEAFKEPRLIDEDRVDAQQARRVFDRLVGYKISPLLWKKVKK
                     GLSAGRVQSVALRLIVEREDEIDRFQAEEYWTLDANLQVEKAAFLARYYGQQGKKQVP
                     ATKEEVDRLLAELKGHDFIVADVKKRERRRRPAPPFTTSSLQQEASRKLGFTTRKTMS
                     VAQQLYEGLKIGKEGVVGLVTYIRTDATRISHDAQMAVRAYISEEYGKQYLPDKPPVY
                     AARKGAQEAHEAIRPTAVDYTPARMKEFLSRDQYRLYKLIWERLAASQMSPAVYDTVT
                     AKINVSTYEFRATGSQIKFDGFMKLYIEGRDVEETEEGGLLPELTAGQKLKLLAFKPE
                     QHFTQPPPRFSEAMLVKTLEEKGVGRPSTYASIIGTLQTRGYIIREKKVFYATELGRI
                     VLEQLLEFFPDIIDVDFTAQLEEKLDQIAEGKEYWVDVIRAFYNSFAIRLKVAEEHME
                     KVEIKPEVSEEVCPNCGRNLVYKMGRYGKFLACPGFPECRFAKPIIKELDVPCPECGK
                     MLVERKTKRGRVFYGCSGYPECEFTTWNLPQKEKCPQCGYLTVLKGKKLQCANQECGL
                     ILNDAAENKKQTKASKKQASRRGSHG"
     gene            complement(32013..33083)
                     /gene="dprA"
                     /locus_tag="VFC74_00615"
     CDS             complement(32013..33083)
                     /gene="dprA"
                     /locus_tag="VFC74_00615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HZK23871.1"
                     /translation="MSDLPYWLALNRVSVLGARRISQLITAYGSAQRAFTVEPGELEQ
                     LGLPVKVSATFLRERGQINPQDEWQRCQRLGISILTLADEGYPQILREIYDPPVVLYY
                     RGDISCLSQFSVAIVGSRKATAYGRAAAEKFAGELASAGVVIISGLARGIDSHAHLGA
                     LQANGLTAAVLGSGLDICYPPENRALREKIVQSGVVISEFPPGAQPKPAHFPMRNRII
                     SGLSQATIVVEAAEKSGALITADCALEQGREVFAVPGSIHSPTSRGCHRLIKEGAAVA
                     ESAADVLQYLGQNVLLETVAALELTAVQALVISALEYEPTHFDQLIKATNLTAAELSS
                     VLVELELAGFLQKLSGNFYLRV"
     gene            complement(33460..33774)
                     /locus_tag="VFC74_00620"
     CDS             complement(33460..33774)
                     /locus_tag="VFC74_00620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23872.1"
                     /translation="MPAQTTLAYFTNFADASAVKNDLENRIYTIAVKSYAAELPDPEL
                     NVSSLMVGFVPDLAHGIFGTNGRVAPTQESGAYLTIIVDEAGHENEIKKITDAHGGTI
                     VS"
     gene            33926..34237
                     /locus_tag="VFC74_00625"
     CDS             33926..34237
                     /locus_tag="VFC74_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-type small acid-soluble spore
                     protein"
                     /protein_id="HZK23873.1"
                     /translation="MANKNRKRLVPEAEKALAKFKMEIAGELGLIDGSTNSWETALDQ
                     YKHEIANELGIDTYVKQKGWQRVPSAVCGSIGGRMGGKIGGNMVKRMIEMAEKSLAGK
                     Q"
     gene            complement(34239..35234)
                     /locus_tag="VFC74_00630"
     CDS             complement(34239..35234)
                     /locus_tag="VFC74_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate dehydrogenase family
                     protein"
                     /protein_id="HZK23874.1"
                     /translation="MYTITLLPGDGIGPGITAATKKVLAATGVPITWEEHVVGEQALA
                     DFGTPLPETVLASIKKNKVALKGPVTTPIGTGFRSINVALRKQLDLYANLRPVRTYPG
                     INTRYQQVDLVIVRENTEGLYAGVEHMVGEDAAESIKIITRKGSERICRFAFTYAQEN
                     NRRKVTAVHKANIMKCTDGLFLSIAREIAQEFPDIEFEDRIIDNMCMQLVQKPENYDV
                     LVAPNLYGDILSDLCAGLVGGLGVTPGANIGQDVAVFEPVHGSAPKYAGLDKANPTAL
                     ILSAVLMLRYLGEHTAAERVENALAAVLHEGKTVTYDLGGSAQGMAMAEAIIAKL"
     gene            complement(35324..36250)
                     /locus_tag="VFC74_00635"
     CDS             complement(35324..36250)
                     /locus_tag="VFC74_00635"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK23875.1"
                     /translation="MNDKELKIINVAYSYLDDSWPLAAVDGVSFTLAPGSALGLVGES
                     GSGKSTLGKCIAGLLCPQRGRIIFRGQEITGNTNRDIQLVFQDSATALNPRMKVSALV
                     EEGLQIQRIGSAKERQKAATELLQTVGLELSLCSRYPYQLSGGQRQRVALARTLILKP
                     QLLILDEPVSSLDVTAGARLLALLHDLKAKHHLTYILISHDLAVVRQLCQRVAVMYAG
                     KLVEIGEVTTVFSQPRHYYTKMLLASHPVPDPRQRKLPETNIYSEPLDPTLRPTGCRF
                     HPRCPAADSHCRRIPPGFKEFAKGHYAACHQA"
     gene            complement(36273..37217)
                     /locus_tag="VFC74_00640"
     CDS             complement(36273..37217)
                     /locus_tag="VFC74_00640"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK23876.1"
                     /translation="MLEIRELKVGFPEQGEILWVVKGSTFSVTAGEVLGIVGESGSGK
                     SMTALALTGLLPPEAIVQGEMIYQQKTFSFDKREQWQKLRGTEIGLVFQDPASSLNPL
                     YPVGRSVWEALRYHLQYSEEKAKKETEKLFSALGILPAAKRVRQYPHQLSGGLKQRVM
                     LAAAICCRPAVLIADEPTTALDVTVQAQILKLLHSYVELQQAALVLISHDLGVIAQIA
                     ERVIVMCQGVIVEEASVYELYQQPLHPYTKLLLTSLPRLDRAILHQVPQASGAAAADK
                     ACVFAKRCQHCQQQCLTKEPVLKTMTPGHLVCCHFPLL"
     gene            complement(37210..38043)
                     /locus_tag="VFC74_00645"
     CDS             complement(37210..38043)
                     /locus_tag="VFC74_00645"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK23877.1"
                     /translation="MRLLNNSSLFFGFTLITGLVMIAILAPLLATHSPYQTDVDNWLQ
                     APSQQHLFGTDRLGRDIFSRVLYGARISLTVGLLATFLSVGIGTLLGLMASFYGGLID
                     MLIMRFADIVLVFPSLLLVLTLVAIFEPAIWLVILVIGCTGWPSVARLVRGEVLRLRQ
                     SDFVASARLLGATNRRIILQHLLPNALGPVLVAATLCVPAAVMTEAGLSYLGLGVQLP
                     MPTWGNMLRDAQAYLRYAWWYAFFPGLFIFLTVFSFHLAGEGLRQALGIDRSTRRNKS
                     A"
     gene            complement(38062..39003)
                     /locus_tag="VFC74_00650"
     CDS             complement(38062..39003)
                     /locus_tag="VFC74_00650"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK23878.1"
                     /translation="MILSLLRRFAQTLLVLFGVSAATFLAMHAAPGHPLQANPELRLD
                     PTAVERWLQLRQLDQPLLSQYLSWLSRVFRGDFGDSLLYNRPVVELIAERLPSTLLLT
                     VTAFCLALFLAIVIGIYAATRQNSWLDRFVCTLSLAGISVPNFWFGIVLIILFAYRFP
                     LLPSVGLRTPGDGSLLDILRHMLMPLFVMVAGNFAHYVRYVRSAILDVLNQDYIRAVR
                     SRGLQEKEILYRHVLPNAAIPIITIAALSLPRLFTGAMVAEYVFGWPGIGRWIITSTM
                     ARDYPVIMTVNLYTASLVAMANFLADALYLLIDPRTR"
     gene            complement(39000..40601)
                     /locus_tag="VFC74_00655"
     CDS             complement(39000..40601)
                     /locus_tag="VFC74_00655"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK23879.1"
                     /translation="MRRILVIFILLFMLFCTGCQQETATSGQSLHVAALTGPLTLNPV
                     FLRDSASAEIAGLLHPQLLVTDPKTLAPQPRLFTSWQLLEDKLTYIFTLHEDACWSDG
                     KPLTTEDIAFTLRVICHPDYTGWLFLPLRYIAGAEGYKQEHRSPQATGELSGVTIIDE
                     KKVQIRLKEPYAPFLAMLTFSPLPAHLLQEVAVADLEADAYSQELTVSAGPYLLADWQ
                     RAEYVHVRANPNYFLGQPDIENIYYRFIPNQEAQLIELLAGKLDIIPTAVRVEDIKTL
                     KQNPDIAIHTNPRLVYDYLVINSQQADSALADKKVRQVLAMLLDRDEVIKNLLLGYGE
                     PLYGPLLPMHFAYNETRKAEKVDLAAARRLLVEAGYAELKLTLIYNVGNTVRENVALM
                     LKEKAASIGMEIKIELLEWEAFLAAVNEGDYDLAILGRGVEADPDLSVHWHSQSPGNS
                     LAYHNEEVDQLTAEAVTTSEHSQRTLLYRQAEELIIKDVPAIWLYVRQAVHATNARLE
                     NFKPHPDSLFFNVHEWRLASEEETP"
     gene            complement(40821..>41407)
                     /locus_tag="VFC74_00660"
     CDS             complement(40821..>41407)
                     /locus_tag="VFC74_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="group II intron maturase-specific
                     domain-containing protein"
                     /protein_id="HZK23880.1"
                     /translation="NIYVRSRKAANRVMASITRFIEGELKLKVNKEKSDVDRPWKLKF
                     LGFSFYPKKGGIGIRVHPKSVAKLKERLKEITGRSNAMSMYQRMVKLRQIIVGWVNYF
                     GIADMRGLAKSLDKWLRRRIRMCYWKQWKKIKTKHNNLVALGVSGYKAWEHANTRKSY
                     WRTAGSPILSKTLTNEYLKKLGLLSISERYSLVH"
BASE COUNT        11273 a        10093 c         8254 g        11787 t
ORIGIN      
        1 gtagtaattt tgaaagttgt aaatgttgtt cttctcaaac aggatcactc ctttgctgct
       61 cgcattgggt tgaggagttt agttaaagaa actggttcca gacctttagt gcgtaaggca
      121 ggaatgacct gtcgtaaaaa ctctggcgtc tgagctgtag gatgcatcaa aataagactg
      181 ccgccctctg ctttggagag gatcttatca agcattactg aaacgccggg cagcatccag
      241 tctaccgtgt ctacagtcca catcacggtg cgctgcttga gtgaagctgc tgcttttaag
      301 acatcggcag acaattcacc gcggtgggga ctaaaataaa ctggtctttt accacaaacc
      361 ttttcaataa cctcagcggc tctactgata tcgtcagttg cagctgctaa tgtcagctcc
      421 tgcatactta atgcgtctga atatccgtga ttaccaagct caaagccggc atcagcaatc
      481 aggcgggcgt cagcttcatt agcttgcacc cagcggccaa ccagaaagaa aacagcctct
      541 gcttgttgtt ccttaagaac agctaacatt tccggcagat attcgttgcc ccaggccaca
      601 ttaattaaaa aggctacttg ctgcttaact ggattcccct ggtagatggg gtgttcaggg
      661 aaggcagtaa gtccaactgc tggtgcagct tgtcgaaata ctggtttcac tgtttcatgc
      721 tcttgtgctg acgatacgcg tgctaaggtc tccgctacat caatcgataa accattcaaa
      781 ccaggtataa ctccattggt agttggatct atcctgcagt cgattggcgg tgtatcttgc
      841 atccttgcca cctgctctat caccgcttta gcttcttctg ttgtcaagcc acccaaatcc
      901 ttgccggcaa gattaacccc tgcacaaata ctagttgctg gacttagaac aggccggaaa
      961 taattagcaa ttatcagcag gagcagaagc ataaaaaata gttgcagcac ttttggcgat
     1021 ttaaggatag cagacacacg gtagcgcatc agatttcccc ctttacttca gacctcgttg
     1081 cgcaagttca gaaaaaggca atacctcata ttcctgcatt aacaaacttt gcaaaatgac
     1141 tggtagggct tctactgcct gtgaggaatt tgtatgcatc aaaattattg caccgttatg
     1201 cagctgtttg gtaatacggc tgataatctt gtctttgcca ggttcttgcc aatcatgcgg
     1261 attaatactc caaacaactg tagtgtaacc tcgcttggca gcaaactcaa aaacacgttg
     1321 atccagttcc ccataaggag gtcgaaatag gtgggtttgc aagttaagct cttcttggag
     1381 tgtttcggcc attaaatcaa attcttttgt taattcctcg tcatctaaat ctgttaaatg
     1441 cttgtgggcg taactgtgtt gtccgatttc gtggccgcga tctataattt cgcgtgccag
     1501 gtcggggttt tgacgtagcc agctgccagt tagaaagaat gtagctctaa tttggtaacg
     1561 gtctaaaata tttaggactt catgcgtaaa ccccggttcc catactacct caaatgtcag
     1621 ggctacagcc cgatcttgag tctcaacttg ataataggca ccttttacta agggcacaaa
     1681 tactccttta gttatttgtc tatgtaggat gaaggttaga agcaataaaa caacgctcaa
     1741 cgataacaaa aaacgttttt tacgtttgcg taaacgctct tctctagaca agcggtcaca
     1801 cctttctcca caggattcgc tttctaaaag gtatgcaagc gaaaaaataa aaatgacaag
     1861 aacacaagca cagaaaagcc tgggctttac ccaggctatt ggactgatta tgttgatgtt
     1921 agctcttact gccacttaac aaaacgtttt tctaggagca ccacggcttg gtacatgagg
     1981 gctgcagcca cgcataagat gatcacactg gccatcacta aatctaagcg aaaaacttgc
     2041 ccgccataga ctattaaata gcctagcccg gcacgcgaaa ccagaaattc accgacaatc
     2101 acgcccaccc atgataaacc gacattgatt ttcaaagcag aaataatagt gggaatactg
     2161 gcaggcagga tcaccttctg cataatttgc ttttttgtag caccaaatgt ttttaataat
     2221 tttattttat ttttatcaac ttgcgaaaaa ccggaataga cgctgaggat cgtcacgatg
     2281 acagaaacca gtagggccat agcgatgata gcccgcatcc cttgccctag ccacacaatc
     2341 ataatggggc ctagtgctac tttaggcaga ctatttagaa ctaccagata tggatcaaat
     2401 acttctgcca aatagtcaga ccaccagagg gcgacagcaa taaatgtacc taacaaagtc
     2461 ccgagagtaa aaccggctac tgtctcaagc agagtaacac ccacatgcgt aaagagatct
     2521 ccgccggcat aaagtgagcg gagtgtttgc acaatccgac taggttgact catgataaac
     2581 ggatcaatcc aacgcagtgt ggcggcaagt tcccagagcg caataaagct gactagcaat
     2641 aaaatttgtg ctgccgtgac gcgccaacga tatcttttgc gagcttggag gtacgccaga
     2701 tgctctgcag aggcagcttg agttgtaaga tcattgctca acatgtacat ccagctcctt
     2761 ccagatacgg ttaaagtaag ctctaaattc tatcgcttca cgacgaccca ggggggagtt
     2821 tatctcgcag gtcaaaacta tgtcatggat gtttttaatt gtccctggtc gtttgcttaa
     2881 aaccactacc cgttcagaca tactaattgc ttcagctata tcatgtgtga ccaaaacagc
     2941 tgttttattt tcgctgctaa taatctcttt cacttcatca gctaccacta aacgagtctg
     3001 gtagtctaag gctgagaagg cctcatccag caataatatt tccggattaa ttgctaaggt
     3061 acggattaat gctgctcttt gacgcatacc gccagatagt tggctgggat aggcatcctt
     3121 aaattcaatc agaccatacg tacgcagcat cttctcggca gctgctttgg ttgcttgatt
     3181 gacattgttt tgtacttcta accccaaaag aacgttatgc cagatcgtac gccattcgaa
     3241 caattggtca tcctgcggca tgtaaccaac taaaggagtt gcgccgctaa ccagctgttc
     3301 ttggatatag acagctcccg cagaaggtgt tattaaaccg gcgagaatat taagtaaagt
     3361 cgtttttccg cagccgctag gaccaacaat actaacaaat tccccttctg caatatctag
     3421 agagatgtct tgcagggcag atatttctcc ttcagggctg taatacttca tgctaacttt
     3481 ttctattctt aataattttt ctgcccccat ctgctctctc ctttcaatta ttaccgtaca
     3541 agttccattg ctttttcagc aaattcattc gttactaaat cagaataggg tgcccgctta
     3601 tccagctcac cagcagtttc cattacatct tgtaagcgag caaaagcttc ttccgtaaaa
     3661 acaggcgtag cagcccaggc atcagtctct ttgtagcggg cagcaacttt tgctaagacg
     3721 acttcatccg agtcgggaaa agaaggagca attacttgag ctatttcttc aggtgtactc
     3781 tcttgaaccc agacctgccc tcggtaaata gcattggtaa acttctggat tatctctgga
     3841 ttttctgcta ataatttttt tcgcgcacaa aaaacagtat agggaatttt tccgctatct
     3901 gcacctactg aagctactac atagccaacg ccttccattt ctagagcgga agcaacaggt
     3961 tcaaataatg tcacataatc accttcgccg ccagtaaagg ctccacccat cagggcaaac
     4021 tggatatggg tcataacttc cacatcttca cccggcatta aaccttgatt tttaagtaca
     4081 tattctaatg tcatcaacgg catgccgcct ttacgtccgc caataatgct tttaccacgc
     4141 actttatccc aagtaaaatt cggatctggt tctctggcca ctaaaaagct tccatcacat
     4201 gttgtcaatt gagcaaaatt aacagcataa ttatcctggc cttggttgtg cacataaaca
     4261 ctcgcctcgg gacccataaa ccctacatct acctcgcctg acaaaagagc tgtcatgacc
     4321 ttatctgctc cttggccagt agtaacctcc aattctaacc cctcatcctc aaaatacccc
     4381 aagtttattg ctgcgtacaa gggagaataa aacacagatc gcgtcacttc acttaatctt
     4441 actttggtta aactatcatc tctttcacag ccagtaagag caagggcaac tagcaatagt
     4501 ggtacaagta catagacaac aacccgcttt tgcacaattg aaaccccttt catgattatt
     4561 tttcataatc ataatattgc cgaagctcat ttatgtgacg gtttcacaca ccaaattatc
     4621 tctttacgca tatctatata agagtgttgc taattttcta tctaaggagt gagcatgtga
     4681 gtaatgcgat gccatctgta ctagtccacg ccgaaaaaaa cacttataaa cttggtgata
     4741 gtgtgatcat aacaattatt gcaaaagccg agggtcagcc tgctcctcat gtaccgctaa
     4801 aactttatgt ctttgatccc acgggcaaga aagttttcca gagctctttg cacacaaaca
     4861 ttaacggtaa agcaatggca aactatcaac taaaaaaaac tctacccaca ggaaattact
     4921 atctggaggc acagaataaa aatagatccg tggccttaag ctcatttatt gtgctgcaat
     4981 aattgcaatc ttgcaaagtg ggactttgtt tgccaggacg ttaaaatatc ccgataaaaa
     5041 aaagaacata aacatttttg tttatgttct tcttgaacgc ttattatcac gcccattatt
     5101 gttgggtcgg cggtcacgtc ggtcatcacg tcgctgttgg ggttcttctt ctatcatttc
     5161 cccttgggca ttaggaattg ctgctcgacg tgagagatcg atacgaccac gatcatcaat
     5221 gccaataact ttaacaggta tttcgtcacc taagttaacc acatcttctg tcttttccac
     5281 tctttcgtgc gctaaacggg agatatggat taatccttct ttgcctggta aaacttcagc
     5341 aaaggcgcca tatttctcca ctctagttac tttcccttga tagatggtgc caatttctac
     5401 atctttagtt aagttttcaa tcatttgctg tgctctcaaa ccaccggcag aatcaacggc
     5461 ggcgatgtag atagtaccat ctgcttcaat ttcaatttgg gcatctgttt ctgccacaat
     5521 tttattaatc attttgccgc caggtccaat cacatctctg attttatctg ggttaatctg
     5581 cattgtaatg atacgcggcg cataaggcga gagttctgcg tgcggttcag ggatcactgc
     5641 tgtcatttga tccatgatat acatgtagcc agttttagct tgtgccaagg cacgggttaa
     5701 aagctctcgc gataatcctt tgattttaat gtccatttgt aaggcagtaa tgccgtcttt
     5761 agtaccggct actttaaagt ccatatcacc taaaaagtct tccatgccct gaatatcggt
     5821 gagaattgca gcatcatcac cctcaatgat caaacccata gcgataccgg aaaccggttt
     5881 tttaattggc acgccagcat ccattaagga aagggttgag ccgcaaacac tacccataga
     5941 agaggagcca tttgactcta ctacttcaga aactaggcgc atggtgtagg gaaattcctc
     6001 ttgtgtggga agaaccggca ttaaggcacg ttctgctaaa gcaccatgac cgatatcgcg
     6061 tcgactagca ccacgcataa agccagcttc gccaacacta tagggtggaa aattgtaatg
     6121 atgcatatag cgttttgact cttccaatcc taaaccatct aaccgttgca catccccggg
     6181 agcacccaaa gtgcaaatgt tcatcacttg tgtttgacca cgccgaaaca aaccagagcc
     6241 atgcgtacgg ggcaaaatgc caacttcgac tgagatagga cgaatctcat cgcttttacg
     6301 tccatctgga cgtacatttt ccttaataat catagtacgc atagtttctt taatcaaatt
     6361 ttcataagca actcgtacgt cccgctcatt atcaggatat ttttctgcaa agtgagtaac
     6421 ggtttcgttt cttaattcat caatagcagt ttctcgtgct tgtttttcaa tagtacgtac
     6481 agcattgtac aacttttcct gagagaattg acgtacttca gccgcaattt cttcagcagg
     6541 cttaaattgg cttactgcca ttttaggctg accgagttca gccatcattt gttcctgtaa
     6601 agccacaata gtacgattag cttcatgacc agccataatt gcttccagga tttctgcttc
     6661 cgttagctca tgggctccag cctcaaccat cataatggca tctttagtac tggcaacggt
     6721 taaatgaagt cgagaagctt ctgcctcggc ctgagtagga ttaattactg gcttgccatc
     6781 tacccaacca actactactg caccaatagg tccaccaaaa gggatatggg agatggataa
     6841 ggctgcagaa gcgccacaga tagacaatac ctctggcgga cagtcctgat caacagataa
     6901 aactgtgctc acaatatgta cggcgttgcg aaaaccttta gggaataagg ggcggcaggg
     6961 cctgtccgtt aaacgagcag ccaaaatagc cttttccgtc ggtcggcctt cccgtttaat
     7021 aaaaccgcca ggaattttac ccacggcata gagtctttcc tcataatcaa ctgttaaggg
     7081 aaagaaatca attccctcac gtggttcagc agaggctgtt gctgtgcaaa ggagcactgt
     7141 atcgccgtag cgaattaaga cagcaccgct ggcctgttgt gcaatccgtc cggtttcaaa
     7201 gcttaattct ctaccggcta attgcatttt ataaactttc atacatatac atcctttcta
     7261 ggttacttaa aaagcttgcg tcttcagtta ctgatggcgc tgttttactg gctgcctaca
     7321 cagcttcgct aagtgcgcag tgatacaggt tcatagctag tataaaaaag cggggtttcc
     7381 cccgctttcg tggcaaatgt gctaccggga aactatttac gcagcgaaag ccttgttaaa
     7441 atagtgcggt aacgctctgc gtcaattttt ttcaaatagt ttagcagacc acgtctttga
     7501 ccaaccatct ttaacaggcc acgtcttgag tggtggtctt ttttatgctc tttcagatgt
     7561 tccgtcaaat aattaattct ttctgttaaa agagcgatct gaacttccgg ggaaccagta
     7621 tcatgctcat gtaagcgaaa actgttaata atatcttgtt tacgagctgt atccatactc
     7681 atgcagcaca cctccttttt ttattagatc cccttaagcc aaggcaacgg cggagactcg
     7741 tttacctgag ccaaggttaa cagttaagat tttaccacaa tgattaggca aaggcaagaa
     7801 agagagcaag aatgacgcag cttgcgctac tgctcttgtt taggcaagtc gcaaatcaaa
     7861 tttttagcct gtcttacgtc agcctggatt tgtgcaacta gagctgcgac atccgtaaaa
     7921 ctcatttctt ctctaatttt ttgtaaaaag gaaacaaata attcctgatc atagagattt
     7981 ttttcagtat caaataaata tacttcaaca gaaactgcat cttttttaaa agttggtctc
     8041 atccccacat tagtaagagc ccagtacttt acttctttta acttcacctg ggttaaatag
     8101 acaccattag ccggcaggat aacttcagca ggaagctgta aatttgcggt aggaaaacca
     8161 agtgtacgac ctctgccatc gccatggatc actgtaccac gtaagaagta aggatatcct
     8221 aacatcaagt tagcttcttc catcttaccc tgctgtagta attggcgaat agctgtactg
     8281 ctaactggtt gatcaaggat agaaatacgc tccactacct gcacagaaaa tcctaaatcc
     8341 tgggctaacc gttgtaaatc ggctggtgta cctatcccac gactgccaaa cgaataatcg
     8401 taacctacaa tcacaccggc aactgaaagt tggcggtgca aaatatcaac aacaaattgt
     8461 tgtggggaaa tggcagcaaa gtcaggcgta aaggggtgaa ttattacata atcaattcct
     8521 gcttccccta acatgcgaat gcgatctgct gcggtattta agagttttgt actttttcct
     8581 ggctttaaaa ttgataaagg atgaggaaaa aagataagga cagcactttt tttgtgatgt
     8641 tctctggcta aagctacagt agtactaata atagctttat gtccgcaatg aacaccatca
     8701 aaatttccta aggccagata aacttgttcc cggtgacaaa gctgaaacga ctcaattccg
     8761 tgtacaattt ccattggctt cacctgctac gaaaaacttt ttgtggccga actgtactat
     8821 tttctaccga ggaaatggcc aacagttctc cccccggccc ataaacacgc attagtgtac
     8881 cctctggcgg caaatcagta aactttagta ccctgccatt ttgaattttt tccgcatcct
     8941 ctgcggtcac gttatacggc agtagctgct ccagtgcaga atctaggggt aataacgccg
     9001 ttgtcaattc atttgccgcc tgtagtgatg cacactcttc taaggtgtat gtttggtcta
     9061 gatgataagg accaacacgc gtacgcacta aaaatgacat atgacccact gtatccagcg
     9121 cttccgcaat ttcacgacac aaagtacgaa tataggtgcc acgtgaacag gacacatcaa
     9181 ataaaatttt ctgtgggtac agctgcaata gttgaagctg ataaatgttg atcgcacggg
     9241 ctttgcgggt aacagtttca ccacgacgtg ccagctgata tagtttttgc ccgtgcacac
     9301 gtacagctga atacatcggc ggtatttgtt gccgtggccc agtaaagtca agcaacaggg
     9361 tttttacctg gttaaaatcc aaaggcggta cagttttttc gtcaattact tgtcccgcag
     9421 cgtcctcagt atctgtagcc gtgcccaaag ttaattcagc tcgataggtc ttgtccatcg
     9481 tcaaaacata ttcggcaacg cgtgtggcct gtccgataca gataggcagc acaccagctg
     9541 ctcccggatc taaagtgcct gtatgtccaa ttttacgaat acccgtcaaa cggcgtaaag
     9601 ctgagacaac atgatgtgaa gtcatacctg gcggtttcag tagattgata ataccattca
     9661 tgcgttaatt tcctcactga ctgacgctag tactagctga agtgctgtag gcaaatcttc
     9721 tgctaaggaa catcccgatg cccgtacatg tccaccgccg ccgaaagaag cggcaacttt
     9781 ggctacatca atctgatgag accggaaacc aactttgatt gttccgtcag cttgttcccg
     9841 aaaaattaaa ccaacctcaa cacctacaat attttttgca tagttaataa ccccgcccaa
     9901 atcttcatcg tgggcttgag cctccttcat atctgcgtct gttaacacaa tccaggctat
     9961 tttaccatca gcagaaaggg tcagagagct aagcgcctga cgaaggatag caagggcagc
    10021 cggtgtctgt aaatcaaaga ttaagggaga gagagctcct gggttgaccc cagcggcgat
    10081 taattcagcc gccatggtat gcgtacgtgc tgtggtactt tcaaacttaa aagaacctgt
    10141 atcggtgcta atagcaacat ataaggcctt agcgatctcc agatcaatct gacaacctaa
    10201 ttcctgcagt aaggcaaaca ctaattcccc agttgccgct gccgtatggt caacatagtt
    10261 taaatgtcca aacaattcgt tggtaggatg atgatctata ttaataattt gtttatcttg
    10321 tatttccccc cagattggtt ggaggcgctt gctgtctgca cagtctagag ttaacacaca
    10381 atcaaaacta gtcgtcggta aacgatgagt atagaatctg tttgtaccga cgagaaattg
    10441 atagcgttta ggtacgtcgt cagcagaaaa catcagtgca gccacatcaa gttgctgtag
    10501 ggctaaaccc atcgctaata ctgagccgat ggcatcgccg tcaggctcaa tatgagcagt
    10561 aatgagcact gcacgacaag ttttaagttt ttgggcaatt tcagctaaac tatgcaccgt
    10621 catcgtcctt cttcaatttc cgtaacactt cactgatatg agctccatag gcgatagatt
    10681 gatccaagtg gaatgttaat tctggtgtat gacgtaaacg gatacgctta ccaattatcg
    10741 agcgaatgaa gccagttgct ttatcgagcg ccgttagtgt gtcttgctgc tcttcatcgt
    10801 taccgaatac actaagataa attttggcgt aacttagatc tcgggtgacc tctacgctag
    10861 tcacactaat catgccactg acgcgagggt ctttcatttc atcgcgcaag atactgctca
    10921 tttctcgttt aatctcttca gcaactctat gtgcccgttg ttcagacata ctaatccctc
    10981 caaaccgcca caagattatg caggcttaat ttcctgtatg gtatatgctt cgatgatgtc
    11041 gccttcttta tagtcgtgga atttttccat taagacccca cattcatagc cagctaaaac
    11101 ttcacgcaca tcatctttaa acctttttag tgtctccatc ttaccttcat gaattacaac
    11161 cccatcacgt ataactctga tgtcggcaga acgtgtaatt tttccttcca gcacataggc
    11221 accagcaatg gtaccaagac gagaaacttt aaatagctga cggacttcgg cctgccccaa
    11281 aattacttct ttaaattgtg gagccagcat gcctttgagt gccgcttgca cttctgctat
    11341 cgcttcataa attactcggt ataaacgaat ctcgacattt tctttttccg ccattttgcg
    11401 cgcattaggc tccggccgta cattaaagcc aataattaaa gcattggaag ctgaagccaa
    11461 catgacatct gattcagcaa ctgcaccgac accgccatga ataacctgaa cccgcacttc
    11521 atctgttgat agtttaagca aagaatcacg cagcgcttct aaagaacctt ggacatcggc
    11581 tttaataata atattcaaat cttttacttc accttgctga atttgattaa acagatcatc
    11641 taaagaaatc ttggctgatt tgttcagctc tacttcacgc tttttatcag ctcgtttttc
    11701 cgcgatttga cgagccatct tatcatcagt gactgcttgt aaatcatctc cagcttgtgg
    11761 cacatcagtt aaacctaaaa tctcaaccgg agtggagggg ccggctttct taattcgctt
    11821 gcctttatca tcaaccatag cgcgaacttt accataaatt gtaccacaaa ttatagaatc
    11881 tccgatgcgt aaagtaccat cttggatcag cacagtggcc acaggacccc ggcctttatc
    11941 tagtttagct tcaactacag caccaagggc aagtttactt gggttggctt taaattctgc
    12001 catttccgct accaacagga tcatttccat caattcatcg aaaccaactt ttttcaaagc
    12061 tgatacttca accatgatgg tgtctccgcc ccactcttcc gcaaccaagc caaattcggt
    12121 caattgttgt tttacacggc cagcattggc cgcttcttta tctattttat taatcgcaat
    12181 aatgatcggc accccagctg ccttgacgtg gttaatggct tctacggttt gcggcatcac
    12241 gccatcatca gcggccacca caataacagc aatgtcggtg atttgcgccc cacgtgcccg
    12301 catcgaggta aaagctgcgt gacctggtgt atctaaaaag acaatttttt tgccttgata
    12361 gtttgcctga taggcaccaa tatgctgtgt aatgccacca gcttcctgcg ctgtcacatt
    12421 agctttacgt atgacatcaa ggactgaagt tttaccgtga tcaacgtgtc ctaagacggt
    12481 gaccacaggt gaacgcggct gtagatcttc ttcattatgc tcttcaacag cttccactag
    12541 ttcagcttca aacttgtcaa ccttaatctc tactgcaaaa ccatattctt cagccagcag
    12601 ctttgctact tctgtgtcaa tagcttggtt aatagcagcc attacgccga agccgatcag
    12661 cttactgatg atctcgctgg cactaacagc taatttgtcg gcaagttcac caactgtaat
    12721 acgaccttct aaaacaactg tattttccgt tttttgctgc tcatcgagta gtcgggcctc
    12781 acggcgtgcc ctacgttctc tcttatctat tttctttttg ggactctggc gtttagcgtt
    12841 gtatctttgt tggcctgtag aactctgtct caccggccgg cttccttgtc cctgagttgg
    12901 tttggacttt tgtgtttggt ctgctgtcga ggcagtgggc tgctttggtg atgcttgctg
    12961 cgactgctct gagcgtccac tattcctttt tggtttagta gctgcgccac tttgttgttg
    13021 aggtttcttc tttgtgtcat gctgcgttgt cttactcttg ttgttggggg aagtttcttt
    13081 tttctgttct ttttctactt ttttattaag ttctttagca tacgcagcgg gcggttctcc
    13141 tttacctgtt aattggtagg tgatgcgctg tgctgcttct ttatcaacaa cactcatatg
    13201 gttctttact tcacaaccaa tttcagttaa taagtcgata atgcgcctac tcggcacatc
    13261 taaatctttg gctaattgat acacccggat ttttgtcatg ggctacacct ccattagttt
    13321 ggcctcctta aacatcactc tatgattaac aaagttttta atcgctcggc aaaaccttca
    13381 tcagttacgg ttacggctgc acgaggggat gtccctaagg ccgctcctaa ctgaatttta
    13441 tccacgaaaa caatcatggg cactgaataa gatttgcttt tatcacgata tctttttttt
    13501 gctgcttcgc cggcatcgct ggctaagagc agtaatttag cttgtccgga gcggattgca
    13561 gctagattgg cttcttcaaa ccaagtaagc ttaccggcac gacgtgccaa gcctaagagt
    13621 gctaatgctt tatttggcat tttattcatc ctccgacaat tgtgcggcta attcttctac
    13681 aactgcggga gatactgctt gttgtaagtt tcgttcgatt ctttttgctt taaccgcctt
    13741 ttccaggcag cttttctgtt tacaaaggta ggtaccgcga ccagcctttt tcccagtagg
    13801 atctaattcc accctacctt cgggcgtacg gacaatccgt acaagctcct ttttgggttt
    13861 ttgttcctgg cagccaatac aggtgcgcaa agggatttta cgttgtttca accaactcat
    13921 ctccctcatc tggagaacta tttgctgctt cgtgttgttc cgccgcctgg ctttcactag
    13981 caatgtcaat cttccaacct gtcagcttgg cagctaaacg ggcattttgt ccttctttac
    14041 caatagccag cgataattgg tagtcaggta ctatcacgtt ggctaccttt tggtctttgt
    14101 gtagcgtaac agaaactact tttgccggag agagagagtt agcaacaaac agttgcggct
    14161 cctcgtccca tttaacgatg tcaattttct ctccgcgtaa ttcattaaca atagcctgca
    14221 cgcgaactcc cttaggcccc acacaagctc ccaccggatc aacatctgta tttttggcat
    14281 acacagctag tttagagcga tgacctgctt ctcgcgctac agatttaatc tctactgtac
    14341 catcaaagat ttcaggcact tctaattcaa acaacctttt taataaacca ggatgtgttc
    14401 tggaaataaa aatttgtgga ccctttgtcg tgcgccgtac ttccgtaata taagctttaa
    14461 gacgttcgcc gggctgataa tcgtcactgg atatttgttc tgttggtaag agcactgctt
    14521 ctgttttccc taaatctaca ataatatttt tctgttcgat gcgctggacg attccggtga
    14581 taatatcttc ttcccggtca acatactcgt caaacaccat ttcacgctct gcttcacgaa
    14641 tacgttggat gacaacttgt tttgctgttt gtgcagcaat acgaccaaag ttttttggtg
    14701 taacttctcg ttctacgata tcatctatgt tataatgcgg gtcgagttcc cgcgcctctt
    14761 ggagtgatat ttctaattgt ggttgtgctg cctctggcac aacagttaag cgtgtataga
    14821 ctactatgcc tcctgtctct ctgtttaatt gtacacgcac gttgcccgct gcgttaaaat
    14881 tacgtttgta agccgagacc agtgcggctt cgatggcttc aaaaagaatt tcctttttga
    14941 cacctttttc acgttcgatc gcagctaggg cagctaaaaa ctcttcattc atttggcaat
    15001 catgcctccc ttccaccgtg ttttactcaa aaactaaatt tgcctttgcc acaagttcta
    15061 aaggaatctt gacaactgtc gcgtccttat tttttgcaag taaaatttcc tgattagcta
    15121 atccccgcag cactcctgta tattgtttag aaccatttag tggagcaaac agttttactg
    15181 tcgccagcct accactaaaa cgttcataat cggctgcttt tttgagcggt cgttcaattc
    15241 caggtgagga tacctgcagc tgataactac tctcaatagg atcaagctgg tcgagttcac
    15301 tgtccatgat ccggctaaaa gcttcgcaat cctcgagtgt cacgccaccc tctttatcga
    15361 ttagaaaacg cagcacaaac tggccgccct cttttacata ctccacatct actaattcaa
    15421 gctgcagatt tgccgctaag ggctctgcca cagcaaataa attatctgta atttttgaca
    15481 ttctcaaccc cccacagagc agtacatggt gatggttaca agtctagtct gtccagatta
    15541 gagcaaattg gccgctaaaa aatactaatt ttttaaactt aatcatggca acacgaaagg
    15601 cgtgctcttt actatttccc ttaagcacgc tgcttagccc tgtccaggtc atcaaccctg
    15661 ctagagctgc caagcgattt tcgaaaagaa aggcttgctt tggcattctg caagcaagcg
    15721 ttaagtaccg tacagtaaaa gttaagagtg ggcggacccc actctccagt aaaaagcatt
    15781 gttttcttag tcattctacc acaattgcca gtaaaccgca agtatggcaa aaagatacgc
    15841 accaagccaa ttgttcagca gattatccct gtagatattc ttgcagggca agcataaact
    15901 cgtctactat tttatcttgg ggaacttttt tcactacctg cccgcgacgg aaaataagtc
    15961 cttcttcttt gccgccagcc acacccaagt ctgcttcccg ggcctcacct ggtccattaa
    16021 cagcgcagcc catcacagcc aaggttaaag gttctttaat atctttgaca cgttcttcaa
    16081 tttgtcgagc taattcctgc aatttaattt gcgtgcgtcc gcaggtggga caggaaatga
    16141 cccgtggacc aaagcgtctg agattggctg ctgctaatat ttctttagct actcgtattt
    16201 cctcaacagg atcagctgtc agggatacac gcaaagtatc tcctaaaccg tcaagcaata
    16261 agctgccaat accaactgct gaataaattg taccactaaa gctggagcct gcctctgtaa
    16321 tccccacgtg ttgtggatat gccacttctc gcgctaaaag gcggtgcgcg gctaccgtca
    16381 aaggtacaga ggaagctttt agggaaatca ccaggttatc aaaaccaagc tcttccacca
    16441 tgcggatatg atccaatgcc gactcaacta aagctgcggc tattactgcg ccatatttct
    16501 catgcaggcg tttttctaaa gaaccggcat taaccccaat tctcaacaca gcatttttct
    16561 ctgctgcccg ttctaccaca gcctgtagtc gctggtgatt accaatatta cctggattaa
    16621 tccgcagttt aggaacaccg gcgtccagag ctgctaaagc aaggcgataa tcaaagtgaa
    16681 tatcagcaac cactggcagc ggcgaaagct caactatttg cggcaagaca gcggctgctg
    16741 cttcattagg tactgccact cttactaact cacagcctgc ttgagctaaa cgtttaattt
    16801 gcagcagggt gccttcaaga tcagctgtat cagtattggt catcgattga ataacaacag
    16861 gcgccccacc gcccattgtc agggagccta tctgcacagc tttagtattt tccctagtta
    16921 tcatcagcta ctcctcaatt aaaaatattt aaacgtacta aatccttgta ggcaatcaca
    16981 atcattaaca caatcagtag ggcaaaacca ataaaatgga taaatgattc ttttttggga
    17041 tccacaggac ggccacgtac cccctccaga gctataaaca ttaaacggct gccgtctaaa
    17101 gccgggatcg gcaaaagatt gataaaacca agatttaggc tgataatcgc cgccagtgac
    17161 agtaaattca taatccctgt acgcgccact tcaccaacga ggctaatgat acctaccggt
    17221 cccatcacat ctgctgagac cttgcccgtt accatttgca gcagactggt agcagtaaag
    17281 ctaataaacc agactgtatg ctctataccc atccgaatag caggcagcag ggcatattta
    17341 cgcgtttccg ccccgatgcc aatacgacct tcaccagttt cagcatcacg ctctggggta
    17401 acagtgacag tggatgtttg attattgcgt tcataaacaa tttccagttc tttaccaggg
    17461 tgactatgaa tcaaagccac cagctcatcc cagttattaa catcctcgcc tgctatggtt
    17521 ataatgcgat ccccgctttg caagcctacc tgtgccgctg ctccttcagg cagcaattcc
    17581 ccgatggcgg tcgaagaata aaccgggacg ccaaggaaag caaaataaat aagtgaaaac
    17641 aaaacaatgg ctagaataaa gttcatcaat ggtccggccg caatcaccag aaaacgtttc
    17701 caaacggcct gcttttgaaa actaccttcc gcttcaacct cctcaggctc ttcaccaact
    17761 aagcgcacaa aaccgcccaa aggaaaggcc cgtatggaat aaacggtttc cccaacttct
    17821 ttatgaacca gacgtggacc aaacccgagt gcaaactcca tcacgccaat cccgactcgt
    17881 ttagcgacaa gaaagtgtcc cagttcatga aaaaagacta gtaaaccgaa gatgattacc
    17941 gaggcgacta atgtcagcat tatttcaccc tttcctttta cccaaaagtt gtgccagttt
    18001 tcgagcttct tgatcaactg cgactaatac atcgaattcc ggcgccataa ttactttatg
    18061 tttattcatt actgattcaa ttatacgggg gattgctaaa aagctaattg atccagcgag
    18121 gaaaagacgt actgcctctt cgttcgctgc attcatcaca gctggtagag tcccgccgat
    18181 tttccctgct tcataagcaa attgcaggca aggaaatttt tctgtatcgg gtggtgcaaa
    18241 gtgcaataat ggcagctgct cccactgcac gcgaggggca tcccctgaca gccgtttagg
    18301 atatgttaag gcatattgaa tgggtacacg catatctggc ctacctaatt gggccaagac
    18361 tgcaccgtca tgaaaagtta ccatggaatg tataatactc tcccgctgta caactacttt
    18421 aattttatcg taggtgatgt caaatagcca atgagcttca attacttcta atcccttatt
    18481 catcaaggtg gcggagtcga tggttatttt agcccccatt gaccagttag gatgtttaag
    18541 tgctgcttgt ggagtaacat gctctaattc agctgtagaa aagtcaaaaa agggaccacc
    18601 tgaggccgtc acaattaatt cttctacagc tgctcttggc tgtccttgca gacattgcca
    18661 aatggcagag tgttcactat caactggtaa aatagcttta ttcacagctg ctgccagcgg
    18721 cattaccaaa tggccaccag ctactaacga ttctttattg gctaaggcca catttttccc
    18781 cgcagccaga gcggttaaag tcggctgcag tccggcaaag ccaaccacag aatttaagac
    18841 aagttcatgc tgcgcgtctg ccgccaggtc acataaggct tctggtcctg cagcaacttc
    18901 cacagctaaa tcgctgcagg ctaattttag ctcctgatac aaacttggct ctgcaataac
    18961 tactttcgcc ggtttataca aacgcgcttg gtcagccaat tctttcacct ggcggtgggc
    19021 tgataagccg tgcaggcaaa atagggcagg atattgctcc acaactgcta aagcctgctt
    19081 gccaatagaa ccagttgaac cgagaatact gatcttcttc atcaaattcc tccctttgct
    19141 gctaaggcca gcagaagata atagcctacc ggtactgcga atagtaagct gtcaagtcgg
    19201 tctaaaaccc cgccatggcc aggcagaaat ttaccagaat ctttgatttt gccccgacgt
    19261 ttcaatgccg attcggccag atctccgcac tggccaacga aggaaatcgc cacgcccagc
    19321 aataatgcgg ttaatggatt taaattaagc aaaggagcga aaactaatga catagctgct
    19381 gcagcaaaca gcaagccgcc gaccgctcct tccagcgact tcttagggct aacagcagga
    19441 attaatttat gacgtcctaa ggtcaggccg caggcataag cgcccgtatc cgaagcccag
    19501 gtagtaacaa gcacacttaa caagagtaaa aacccattag ctgtggcccg cagccacaga
    19561 aaaaaggcta gtaaagaggt gatatataaa gccccccaga gcaagatcat taattcttga
    19621 taatcttgag gatacgcaaa aacagcgaca agattaagta caataaaaaa aactattaga
    19681 gcaaaaaaat aagcttctgg gtttgctaga ccagctactg tcaacaacag gatattccct
    19741 gcgtaaccta gagtacggac gagtctattt tccaatgaac aaagtgtata aaattcgcgc
    19801 agtgccaagc tgcttatgaa aataattgcc acaaaaatgg ccacaccgcc ctgccaaatc
    19861 agcaagacga caagcggtat acccaccaag gcactcaaaa gccgctgtct gaacattaaa
    19921 tccctcccaa tccaccaaaa cgcctgtcac gctggcgata agcttcaatg gccgctaaca
    19981 agtgttctgc tttaaaatcc ggccagtaaa tatctacaaa ccaaagttcg gcataagcta
    20041 attgccatag taaaaaatta ctgaggcgaa cctcgccact ggggcgaatc aataaatctg
    20101 gatcgggtaa ttcagaagta tacaagtgtt gttttatggt ctcttcagtt atttcttgta
    20161 catgcaaact gcctgcaatc accgcttgcg cgataccttg tacagcatgc agaatttcag
    20221 cgcggctgcc gtaatttaaa gcaaagttaa gaatcatgcc agtattagca gcagtgctgg
    20281 tcatggcttg gtcaatagcc gctaaagtat aatccggtaa tccttcacga gcgccagaca
    20341 tcattacacg cacattctta gcgattaatt ctggcaaatc tgtacgcaaa aattcactgg
    20401 gcagccgcat taaaaaattt acttctgctg gaggccgatg ccaattttca gtggaaaaag
    20461 cataaaccgt gagcacacca agacctatct cgttagcggc acggatgatc cgacgcaaag
    20521 ctaccatgcc ggcatggtgg cctgcggtac gcggcagacc gcgtttggca gcccaacggc
    20581 cattaccatc cataataatt gccacatgac gtggtatttc atcactagta tttggttgag
    20641 gtattttttt ttgaaaaagc cttttcagtg aaaaagacaa tctatccacc tcgagtcata
    20701 tagtaacagg ccccctcaga cgagggggcc acaatcttca gccgctacag tcagttctaa
    20761 cacagtttta tcctgtgtaa gacgtactct cactaccctt tcgctcccct ggtttatctt
    20821 tttgggtggt gaagtgtgct gtagggaagg ggttagaccc caactttctg tgatgcgtac
    20881 agcctcggca agcgggtaag ctaggagatc cgggatgcct gtcctcttgt gcattttaga
    20941 cctccataat ttccttttct ttatcggcaa gcacattatc tgcagcttcg atcattttat
    21001 ctgtgagttt ctggatctcg tcctgtgctt tatgtaattc atcttccgta atttcatgtg
    21061 ccttttccat attcttcgta gtatcattag catcgcggcg aatattacga atggcgactc
    21121 taccgtcttc agccttcttc ttactaattt taactaattc ttttcttcgt tcctccgtca
    21181 attgcggaat agacagacga ataagtgaac catcactatt aggcattaag cctaaatctg
    21241 atttcagcag cgcacgttca acttcgccga tcaccttttt atcccagggc tgaatgacca
    21301 acagacgcgg ttcgggtaca gaaatattag caagttgatt aataggcgtt tgggcaccgt
    21361 agtattccac gacgatccta tccaaaagag ctggtgttgc ccgacctgtc cgcagtgtgg
    21421 ataaatcttt ctttaaagcc tgcactgctt ttttcatcct ctcctcggca tcacggtaaa
    21481 cctcgttaag catgcttatt ccccctttac aaatgtccca attttaacac ctaatagtac
    21541 ccttttaata ttaccggttt tatttaggcc aaaaacaatc aacggaatct tattatccat
    21601 acatagcgat gcggccgtgg aatccatgac acccagacct ctatttaaaa gatcgatgta
    21661 cgttaattcg gtgaattttt ttgctgtggg atttgtgagg ggatctgcat cataaacagc
    21721 atcaacttta cctttagcga gtagaataac ttcagcttca atttcagctg cgcgcagtgc
    21781 tgctgttgta tcagtagaga aataggggtt tcctgtgcca ccggcaaaga tgaccacacg
    21841 atttttttct aaatgtcgaa tcgcccgccg acgaatatag ggttctgcca cttgctgcat
    21901 ttcaatagct gtttgcacac gtgtatcaac gccacgtgat tccaaggcat cctgcaatgc
    21961 cagggcatta aggacagtgg cgagcatgcc catgtaatca gcagtggcac gatccatgcc
    22021 tcgttcacct gcaggtgcac cacgccaaat gttcccaccg ccaacaacta cggcaacttc
    22081 cactcccatt ttccaaactt cttcgacttg ggctgcaatc tcagatagaa catccgagtc
    22141 gatgccaaag ccacgttcac cagctaaagc ctcaccactt aatttgagca caattctttt
    22201 atatactgct ttctccatag aagccgtatg acctccattc ctaattttcc acataagatt
    22261 gtattatcct gtaaatattc tgtaaaaaaa gaacaccgaa gtgctctttt tatagttgtc
    22321 catcacttaa ttaagactta gtcatcgcgg ctacttcatc agctaaattg catccttttt
    22381 tctctaagcc ttcgcccatt tcatagcgaa caaaacgacg aatggaaatg ttctcgccga
    22441 ttttagctat tttatcctgc aataaacctg taatggtgac atcagtatca cgaataaatg
    22501 cttgttctaa taagcagttt tctgtataga atttgctaat acgaccttca actattttct
    22561 cgataatctt ttccggtttc ccttcgtttt tagcctgagc tcgaaggatt tcacgttctt
    22621 tgtccagaat ttctgcaggt acgtcttcac gcgctaaata ttgtggatta gtggctgcaa
    22681 tctgcatcgc aacatcacgc acaaaattac ggtattcatc tgttctggcc acaaagtctg
    22741 tttcacagtt tacttccaag aggacaccaa tttttcctcc catatgtata tatgaatcaa
    22801 ctaatccttc agcggcaata cgaccagctt ttttagctgc cgcagataag cctttttcgc
    22861 ggagaaattc agcggccttt tctaagtcac cgccagcttc agtcagtgcc tttttacaat
    22921 ccatcatgcc agcacctgtt ttttcgcgta actctttaac aactgatgcg ggaatcataa
    22981 aaaaacctcc caatgtttga tttttaaaaa agcttcgttt tatatttcgt ttgctatttt
    23041 tatccttatc ccgtgctttc agtacacacc tagctagtgt taactgccaa gtaattgtca
    23101 tcgcaagtat aaagaaggcc gggcacacgc ccgaccgtaa tttatgactt tacaattcta
    23161 cagcgggcgt gttgtcttcg acttctagtt gttcctcaac cacaacgtga tcctgacggc
    23221 cttccaggcc ttcgaggact gcgtccgcca tacggcctga gattagttta acagcacgga
    23281 tggcatcatc attaccaggg ataatatagt cgatttcatc tggatcacag ttggtatcaa
    23341 caatggcaat gatggggatg cctaattttc tggcttccgc aacagcaatt ctttctttgc
    23401 gtgggtctac gatgaaaagc gcatcgggaa gggtatgcat attccgaacg cctgaaagaa
    23461 atttattcag cttgtcatgt tcgtgacgaa gttgaatcac ttccttttta ggcaggacac
    23521 caaagatacc gtcttcttcc attttctcta actcaatcag gcggttaatc cgtttacgaa
    23581 ttgttttaaa gttcgttaac atcccaccca gccagcgttg gtttacataa aacattccac
    23641 aacgttcggc ttcaagttga attgactcct gcgcttgctt tttggttccc acaaacataa
    23701 ttcttctgcc gtctagggcg agatcacgca caaaacggta gatatcgtct aacattctga
    23761 cggtttgctg aaggtcaata atgtaaatac cgtttctctc cgtaaaaatg taaggtttca
    23821 ttttggggtt ccaacgacga gtctggtgtc caaagtgaac gccagcttct aatagctgtt
    23881 tcatggatac tacgggcatg ctcctgcacc tcctctctgt ttttcctccg ttccttcatt
    23941 tgtttgcagc actgtcaaga cagcacagct gcaagcatca ggtaacgtgt gtatttaata
    24001 ccgttttgta gtataacata gtttgcctgg tttggcaatg cggtgctcaa gataatttcc
    24061 ctattttact tgcacacgta ctactaccgg gatcttgtca aatatataga cgggaaaacc
    24121 tttagcactt aattctccac tcatggtttg tttaaccatt gtataaacat tatctccaaa
    24181 ctgttcagct agtatttgtg tattaatgcc atctaaaatt tgcccagtcg cgttttctac
    24241 attcgtacgc atcttttccc tcaattgcgt catcagctct tccggcatag aaaaaataaa
    24301 accggcgatt tccatgcgtt ctgaaaactg atcatgcatt tcagtttcca cgatatgtgg
    24361 tatttccgct ttggcgtcag taataatttt agggagttcc tgttttgctt gggaaactac
    24421 ctctaattca atcgtgcgcg ccaacgcttc actgtctaag tgcacagtca ccccataagc
    24481 agcaacgtag tagagggcca tggcactaaa taccaccact acagctaaag cactgcctac
    24541 gccaaataaa tatgactttc tatccaagac ccaacgatat ttacttcttc ccatttttca
    24601 cctcttcaga actagtatgc atgtatttgc ctataacaat atagtataac aaatcttgct
    24661 gtaaattatg actaacataa ttttttcagc taggctcacc tggttaaggt gggaagcaaa
    24721 atatcctctt gaataaatga aagacgcgcc agctcaaagc ttgcgcgcct acttttacat
    24781 ggctacggca tttctttttt ctatttgttc aagtaaatag ggatttttca ctttaatgta
    24841 agtacccttc attcccaagg aacgggactg aatcacaccg gcactttcga atttacgcag
    24901 cgcattaaca attacggaac gtgtaatgcc aagctgatct gcaatcttac tggctactaa
    24961 aatcccttca tcaccattga gctctgcaaa gatgttctct acagcttcca gttctgaata
    25021 agaaagtgta ccgatagcca tctgcacaac agctttatta cgtgcttctt cttcaatttt
    25081 atcagcacga atacgtagta cttccatacc gatcaccgtg gcaccatatt ctgccaatac
    25141 tagatcttcc gatctgaaat ctttgccgtt ccgggccagt aggagtgtgc ccagtcgttt
    25201 tccgccgcct aaaataggga taatagtggt agtattatct ttaaacagag cgtctgtttc
    25261 gtcatgctgc aaattcactt ttgattcatc atagctaagc agctgtccat tgtaatcagc
    25321 tggtaaggtg tcaccttcac ttagcatttc accattaaac tcatcaattg ccgatatccc
    25381 gagaattttt ccactacgat caacaatata gacattggca gcaatcacat ttttcaaaac
    25441 ttccgccaca tctttaaaat ctacgtgctg acctgccgtt ttctgaagaa ttttattaat
    25501 gcggcgggat ttttccaata aggcttcaat taccatattg atcattcctt tctctttaga
    25561 attacagaat atacctgctt aaatccttat tttgtacaat ttgctgaatt ttatcccgaa
    25621 tgtattgtgc tgtgatcgtt acttcttgac ctgcgatctc tggcgccgca aaagacaggt
    25681 cttccaatac tttttccaat attgtatgca aacgcctggc cccaatgttc tccatctctt
    25741 gatttaggag gcaggcggtt ttagcaattt ctccaatggc atcatccgtg aaaattattt
    25801 ttacaccttc tgtggccaaa agttcagtat actgcttaat taaggcattt gccggttcgg
    25861 tcaatatgcg ataaaaatct tcctgtgtta gactatccag ttcaacgcgg atcggaaaac
    25921 gtccttgtaa ctcaggaatt agatccgatg gtttagctac gtggaaagct cctgcggcaa
    25981 taaataaaac ataatctgtt tttacaggac catatttagt gatgaccgtt gatccttcaa
    26041 cgatcggtaa aatatctctc tgtacacctt cgcgtgagac atcaggaccg ccttttaagt
    26101 ctttacccgc gattttatcg atctcgtcta agaaaacaat tcctgattgt tcagctcttt
    26161 ctatcgcttc tgcatgaact tcatccatct ctattagttt ttctgcttct tcttgctgca
    26221 aaatctttct tgcttgcgct acagtaattt tacgtgtttt tctccgcttg ggcaaaaatt
    26281 gccccataat gtcctgaaag ttaatgccca tatcttccat accggagcca ggaataattt
    26341 ccatcatcgg tggactagcc tcaacttcca tttctataag ttcattttct aagctgccag
    26401 catgcaaacg ttgtgccagt atggttcttt tttcagtacg tacttgctgc tcgctctgcg
    26461 aataatgggg atcctcttca ttttcttcct gctcctctgg accggagaac aacaacgtta
    26521 aaggattttg tactttcttt tctttggggg gttgtggaag taacagttct aaaagacgtt
    26581 cttctgccag ttgttgtgct ttttcttcca cagcgcccag tttctctgcc tgaattatcc
    26641 gtactgaagt ttccactaag tcacgcacga ttgtttccac atcgcgtccc acgtagccaa
    26701 cttcagtaaa ttttgtcgct tcaactttaa cgaaaggcgc atttaccaac ttcgctaaac
    26761 gccgagcaat ttctgttttc cccacgcctg tggggccaat cataataata ttcttgggaa
    26821 atacttcttc acgaaagtca gcggctaata gtttgcgtcg ataacgattc cgcaaggcga
    26881 cagccacaca ttttttcgcc tctttttggc ccactatata tttatccagt tctgcaacga
    26941 cttgcctggg ggttaactct tctctattta actgtttttc catgtaatta tagtacctcc
    27001 agcacaattc tatcattggt atagatacat atttcaccag cgatttccaa agcacgtctg
    27061 gcaatctcgc cagcatccat tttagtatac cgacttaagg cacgcgccgc tgctaaagca
    27121 taaggactgc ctgaccctat agctgttaca ccgtcatccg gctcaataat ttcaccattg
    27181 cctgagatga ctaaaatgcc agttaaatcc ccaacaataa gcagggcctc taaacgacgc
    27241 attacacggt cagttctcca gtctttagct aactcgatgg cggcgcgacg caaattacct
    27301 tgatattttt ccaactgctc ctcatatctt tcaaagagcg tgaaagcatc tgccactgaa
    27361 ccagcaaaac ctgccagcac cttgccttgg taaatacgac gaactttaac ggcttggtgc
    27421 ttcatgattg tcttatcgcc aaaagtaact tgcccatcac cagcgagggc acagcgacca
    27481 tccttgatca cagccactat tgttgttgca cgaaacataa tctactcctt acgcccgagg
    27541 gtgggcacca tgatatactt cttttaagcg gtcacgtgtc agatgagtat agatttgtgt
    27601 tgtagaaaga ttaatatgtc ctaataattc ttgaaccgct cgtaagtccg caccggcatt
    27661 cagtaagtgc gtggcaaaac tatgccgaat actatgagga ctaatgccgt gcgtcaaact
    27721 ggcctgctgt acatatttgt ctacaatccg cctaacccct ctatctgaga gccgcgtacc
    27781 atacttgttc agccacagtg cttgctctcg ctctccagca ctgttcttac gcagcagctg
    27841 cggtctggct tcatgcagat agcgctgcac tgcgttacaa gcgcaacgac ctagtggcac
    27901 cattctttcc cgtgcgccct tacctttaac aataaactga cccaggtgta aatccatcgt
    27961 ctgcacatta agcgccacaa gttcacccac gcgcacacct gaggcgtaca taacttctaa
    28021 aagagcagcg tctcgctgtc ccgctggggt tttagcatca ggggcgtcta atagacggat
    28081 catttcttct ggataaagaa agatgggcag cttttttcct acacggggag tggacacact
    28141 atgccaagtt ttatcctcaa gcaattcctc cctatttaaa taacgtagaa aagaacgaat
    28201 agcagaaagc ttgcgggcaa tcgtacgccg ctcgtaagct tttttttgta gtaaggctaa
    28261 atattgccgt aaggtcatat aatcgattga cgatatttca atagcttctt tttctaaaaa
    28321 ggaggcgaat tgcgctaaat cctctgcata attacggata gtatggtaag atgcgttttt
    28381 ctctacttgt aaataagata aaaaaccatc tatccagtgt atcattcgac gaaaatctcc
    28441 tttttcaaaa tattttaaca tattgcagta ccattggcaa gtaagaaatg gtgaaaggtg
    28501 aaatttgtgt caatgttaat aattcgttaa cgtcgttttc tgccatgctt ccaattcggt
    28561 taaggcgcgt tcggctaagg ccaattttct ttttttcttt ggtatgcgct gagcaagtgg
    28621 tggcagcaga ccaaagttaa cattcatagg ctgaaaatta tttgggtctg ctgtagtaat
    28681 atagtgcgta agagctccgt gtgcagaggc agccggccaa atttggggct cttttgctgc
    28741 tagctggcgt gcggcgttaa gaccggcaat taggcccata gcggccgact ctacataacc
    28801 ttctactccg gtcatctgac cggcaaaaaa gagttgagga ttgccggcaa actgtaaagt
    28861 tgcctttaat acttggggag cattgatgta ggtgttacgg tgcatgacgc cgtagcgtac
    28921 aaattctgcc ttttccaaac cagatatcat accgaaaacg cgcttttgtt ccggccagcg
    28981 caaacgagtc tgaaaaccaa cgatattgta aagggacgct gcagcattat cttgacgtaa
    29041 ctgcaccaca gcatagggtt gtttacccgt atggggatct tttagcccga caggcttcat
    29101 gggtccatac aataaagttt caaaacctcg taccgccatt acttcaactg gcatacaacc
    29161 ttcgaaatat tttttttctt ctacactatc gtgaccttcg tacacttcag cggtggttaa
    29221 ggcttcataa aaacttttat attcttcctt gccaagcggg caattcagat aatcggctgt
    29281 tcccttatca taacgggagg ctttgaagac acgctccata tcaatagatt ctgctgtcag
    29341 aattggtgct gccgcatcat aaaaatgcag cgatgattgc ccggtaagtt caccaatttt
    29401 ttgtgctaaa gtttctgaag tcagcgggcc tgtggctata attacaggac cgtttgtcgt
    29461 ctcggggatt tctgttactt cttgctcgcg acaagtaata agtggatgat tagtaatcgc
    29521 ctctgttact ccttgcgcaa aagcttcacg atcaacagcc aaggcaccac ctgccggcac
    29581 agcatattta tctgcctgcg ccattatcag cgaattttgt cgacgcattt cttcctttaa
    29641 caaacctaca gcattttcta gagaagcgcc gcgaaaggaa ttactgcaca ccaattcagc
    29701 taaagcggcc gtatggtgag caggcgtcat cttctgggga cgcatttcca gtagtaacac
    29761 ttcacagccg ctttgggctg cttgccaagc agcttcagct cctgctaaac cgccgccgac
    29821 gatagtaatt ttagccatgc gaacccctcc tagatgcttg ctttttacta gctttagttt
    29881 gttttttatt ttctgcagcg tcatttaaaa tgaggccgca ctcttgattg gcacattgta
    29941 gttttttacc tttaagcacc gttaaataac cacactgtgg acatttttct ttttgcggca
    30001 gattccaggt ggtgaattca cactcgggat aaccgctgca gccataaaac actcgtcccc
    30061 gttttgtttt tctttctacc aacattttac cgcattccgg gcaggggaca tccagctctt
    30121 taataattgg tttagcaaaa cggcattcag gaaagccagg gcaggccaag aatttgccat
    30181 accgtcccat tttgtatact aaatttcgtc cacagttggg acagacttcc tcacttacct
    30241 caggtttaat ctctactttt tccatatgtt cttccgccac tttcagacgg atggcgaaac
    30301 tgttataaaa agccctaatc acatcaaccc aatattcttt tccctcagcg atttgatcta
    30361 gtttttcttc taactgcgcc gtaaagtcta catcgataat gtcgggaaaa aactccagta
    30421 attgttccaa gacaattcgt cccagttcag tagcgtaaaa aacttttttc tcccggatga
    30481 tataacccct cgtctgcagt gtcccgatga tggaggcata agtactagga cgccccacgc
    30541 ctttttcctc taaagtcttg actaacattg cttcactaaa gcgcggtggc ggttgtgtaa
    30601 aatgttgttc tggtttaaat gccagcaatt ttaatttctg cccggccgtt aactcaggca
    30661 gcagaccgcc ttcttccgtt tcttcaacat ctcgaccttc aatatataat ttcataaagc
    30721 catcaaattt tatttgtgac cctgttgccc tgaattcata cgtactgaca ttaattttag
    30781 ctgtaactgt gtcatagacc gcaggactca tttgactggc agctaaacgc tcccagatta
    30841 acttatacaa gcgatattga tcacgggata aaaactcttt catccttgct ggcgtatagt
    30901 caactgctgt cggccgaatg gcttcatgcg cttcttgcgc tccctttctg gccgcgtaaa
    30961 ccggtggttt gtcaggcaga tactgcttgc catattcctc gctgatgtaa gcgcggactg
    31021 ccatttgtgc atcatgagag attctggtgg catctgtacg gatataggtt actaaaccca
    31081 ctacaccctc tttgccaatt tttaatcctt catatagctg ctgggctaca gacatcgttt
    31141 tgcgcgttgt aaaacccaat ttccgggaag cctcttgctg caggctgctg gtcgtaaatg
    31201 gtggcgccgg tcttcttctg cgttctcgct tttttacatc ggctacgata aaatcatgac
    31261 ctttaagctc tgcaagcaaa cgatccactt cttctttcgt cgccgggacc tgttttttac
    31321 cctgctgacc atagtatcga gcgagaaaag cagctttttc tacctgcagg ttggcatcta
    31381 gtgtccaata ttcttccgct tggaaacgat ctatttcatc ttctctttcc acaattaagc
    31441 gtaacgctac agattgaaca cgaccagctg aaagaccctt tttgactttt ttccagagca
    31501 atggtgatat tttataaccc accaaacgat caaacacgcg gcgtgcttgc tgcgcatcca
    31561 cacgatcttc gtcaattaag cgcggctcct taaaagcctc ttttacggct ttttttgtga
    31621 tttcatgaaa ttcaactcta cagggtatat cctgctgcag cttaagtgcc cgcgctaaat
    31681 gccagcagat tgcttcgccc tctcgatcgg ggtcagcggc taaaaatact tttttggcat
    31741 ccttgctggc atttttcaat tcttttaaga ctttgccctt gcccctgatt gttatatact
    31801 ttggtgtaaa attgttttct atatcaatcc caatttggct tttaggtaaa tctatcacat
    31861 gacctaaaga agctataacc ttgtagcgtg agcctaaata ccttttaatt gctttagctt
    31921 ttgtcggtga ttcaacaacc accagataat ctgacatttg gcacccccaa aacatcctct
    31981 actttctact acatatacca atttcataat ccctatacac gtaaataaaa gtttcctgat
    32041 aatttctgca aaaaaccagc taattctagt tctacaagca cacttgacaa ttccgccgcg
    32101 gttaagtttg ttgctttaat cagctgatca aaatgtgtgg gttcgtactc taaagctgag
    32161 attaccaaag cttgaacagc agttaattct agcgcagcca ctgtttccaa caacacattt
    32221 tgtcctagat attgcaagac gtctgctgca ctctcggcaa cagctgcccc ttctttaatt
    32281 aaacgatgac aaccacggct ggtagggcta tgaatacttc ccgggactgc aaaaacttcc
    32341 cggccctgct ctaaagcaca atccgcagta atcaaagcac cactcttctc tgctgcttcc
    32401 accacaattg ttgcttggga taaaccgcta ataatccggt tgcgcatcgg gaaatgagcc
    32461 ggtttaggtt gtgccccggg cggaaactca ctgatgacaa caccactttg cacaattttt
    32521 tctcttaaag cacgattttc cggtggataa caaatgtcca ggccgctgcc caaaacagca
    32581 gctgtgagcc cgtttgcctg taaagcaccc agatgggcgt gactatcaat gcctcgtgcc
    32641 aggccgctga tgattactac accagcactg gccagttctc cggcaaactt ttctgcggca
    32701 gctcgtccat aggctgtagc tttacgcgaa cccacaatag caacgctaaa ttgcgataaa
    32761 caactaatgt caccacgata atataaaaca actggtggat catatatttc acgtaaaatt
    32821 tgcggataac cctcatctgc caaggtaagg atactgatcc ccagccgctg acaacgctgc
    32881 cattcatctt ggggattaat ttgtcctcgt tcccgtaaaa aggtggcgct aactttcact
    32941 ggtaaaccta gctgctcaag ttctcccggc tccacagtaa aagctctctg ggcactgcca
    33001 taggcagtta tcagctggct gatacggcgt gcacctaaca cggaaacacg attcaaagcc
    33061 aaccaatacg gtaaatcact catcatttta cccctttcgg cactttgctt tagattctac
    33121 gcaacctgaa ggattcctgc acatttccat ttttttgcac aacagaattt tatgcactta
    33181 acagcagtta agcctgaact ggtccggctt aatactagcc agcttaagtt taagcgcggt
    33241 tttaggcagc caagcatttt ttgtattata ccatacgcac tcaacacgaa caagcgttcc
    33301 gcctactttt gcgtaattat tcagaaactt aactagctgc taatcgtcat agtcatagag
    33361 gaaataatag cgacgcctga gggcgtcgtt tttctctaga ggcatttttt actagtgaca
    33421 gagaactaaa caaggctgca agctgctccc tatcgcctat tacgagacaa tggtgccgcc
    33481 atgtgcatct gttatttttt taatttcatt ttcatgtcct gcttcgtcta caataatcgt
    33541 caaataggct ccgctttcct gagtaggcgc tactctgcca ttggtaccaa aaataccatg
    33601 tgctaggtct ggcacaaaac caaccattag agaagacacg ttcagctcgg gatctggcaa
    33661 ttctgcagca tagctcttaa cggcaattgt atatattcta ttttccaagt cattctttac
    33721 tgcagaagca tcagcaaaat tagtaaagta agctaaagtt gtttgtgcag gcatctcatc
    33781 agctcctttt ttctattttg ccaagataaa gctttcctat gcttgcagta tttcgctagg
    33841 caagtaaaat tttgttgtgg ataggatcac gcaactagcc aaaactagag gcaaagattt
    33901 ataaaaaata aggatggtga aaaatatggc caacaaaaac agaaaacgcc ttgttcctga
    33961 agcggaaaag gcgctcgcta agtttaaaat ggaaattgca ggtgaactag gtctcatcga
    34021 cggcagcact aactcttggg aaacagcctt agatcagtac aaacacgaaa tagctaatga
    34081 attgggtatt gatacatatg taaaacaaaa aggctggcag cgtgtcccct ctgctgtctg
    34141 cgggtctata ggcggccgca tgggcgggaa aattggcggt aatatggtga aaagaatgat
    34201 agaaatggca gaaaaaagtt tagccggcaa acagtaaact atagtttagc aataatagcc
    34261 tctgccatgg ccataccttg agcagagccg cctaaatcgt aggttacagt cttaccctcg
    34321 tgaagtacgg cagctaaagc attttcaacg cgctctgcag cagtatgctc gcccaaataa
    34381 cgcagcatca atactgcaga taaaattagg gcagttggat tagctttatc tagaccagca
    34441 tatttaggcg cgctgccgtg aactggttca aaaacagcaa catcttgtcc aatattggct
    34501 ccaggagtca ctcccagccc tccaaccaag cctgcacata aatcagaaag aatatccccg
    34561 taaagatttg gtgctacaag aacatcatag ttttcgggtt tttgtactaa ctgcatacac
    34621 atattgtcaa taatccgatc ctcaaactca atatcaggaa actcctgggc aatttctcta
    34681 gcaatagaca aaaatagtcc atctgtacat ttcatgatat ttgccttatg aaccgctgtt
    34741 actttacggc ggttattttc ttgagcatat gtaaaagcaa aacgacaaat acgctcactg
    34801 cccttacggg tgataatttt aatgctttca gcggcatctt cgcccaccat atgttcaaca
    34861 cccgcataca gaccctctgt attttctcgc acgatcacca agtctacctg ctgatagcgt
    34921 gtattaatac caggatatgt acggactggg cgtaaatttg catacaaatc gagctgcttg
    34981 cgcaaggcaa cattgatgct gcggaagcca gtaccaattg gcgtagtaac tggccctttt
    35041 agcgcgactt tatttttttt aatcgatgcc aacactgttt ccggtaatgg ggtgccaaaa
    35101 tcagcgagag cttgttcccc taccacgtgt tcctcccaag ttatcggcac tcccgtcgcg
    35161 gctaagacct ttttggttgc tgctgtaata ccgggaccaa tcccgtctcc tggcaaaagc
    35221 gtaattgtat acatttgcta gatcctcctt ttgatcaagg catgtccatg tttgcttact
    35281 attatagcaa tgacctagta cgctgtaaat ggctgcacca gccttaagcc tgatggcagg
    35341 cagcataatg tcccttagca aattccttaa agccaggggg aatacgccgg cagtgggaat
    35401 ctgcagctgg acagcgggga tgaaagcgac agcctgtagg acgcagcgtc gggtcaagcg
    35461 gttcactata aatattcgtt tctggtagtt tccgctgcct ggggtcaggc accggatgtg
    35521 aggccaaaag catttttgtg taataatgac ggggttggga gaaaacagtc gtgacctcac
    35581 ctatttcgac taattttccg gcatacatca cggctacccg ttgacatagc tgccggacga
    35641 cagctaaatc gtgggaaatt aaaatatagg taagatggtg tttagctttt aaatcatgca
    35701 aaagagcaag taaacgtgct cccgccgtca catctaacga ggaaacaggt tcatctaaaa
    35761 ttaatagctg tggttttagg attaacgttc tggccaaagc tactcgttgt ctttgccctc
    35821 cactcaattg atagggataa cgtgagcata aggaaagttc cagcccaaca gtctgcagca
    35881 gttcagtggc tgctttttga cgctcctttg cgcttcctat ccgctgtatc tgcaggcctt
    35941 cctcaactaa agccgatact ttcatccgag gattaagagc agtagcacta tcctgaaaaa
    36001 ccagctgaat atctctgttt gtgttcccgg ttatctcctg ccctcgaaag attatccgac
    36061 cccgctgcgg gcaaagcaag ccggcaatac atttgcccaa agtgcttttg cctgaacctg
    36121 attcacccac taatcccagg gcactgcctg gcgccaacgt aaatgataca ccatcaacgg
    36181 ctgccaacgg ccaggaatca tctagatagc tgtaggcaac gtttattatt tttaactctt
    36241 tatcgttcat ctttaccctc tttttactgc gattatagca aggggaaatg gcagcagacc
    36301 aaatggcctg gtgtcatagt tttaagcaca ggctcttttg tcaggcattg ttgttgacaa
    36361 tgctggcagc gtttggcaaa aacacaagct ttatctgctg cggctgctcc gcttgcttgc
    36421 ggtacctgat gtaagatcgc acgatccaaa cggggcaagg aagttagtaa aagcttggta
    36481 tagggatgta atggctgctg gtaaagttcg taaacagaag cctcttccac aatgacgccc
    36541 tgacacatca cgataacccg ttcagcaatt tgtgctatca cccccaagtc atgcgaaata
    36601 agcactaaag ctgcctgctg cagctcgaca taggagtgta acaactttag gatctgtgct
    36661 tgcaccgtta cgtccaaagc agtggtaggt tcatcggcaa tcaatactgc aggacggcag
    36721 caaatagcgg cggccagcat cacccgctgt ttaagtccac cacttaattg atgtggatac
    36781 tgccttactc ttttagccgc gggtaggata cccagcgcag aaaataactt ctctgtttct
    36841 ttttttgctt tttcttcaga atactgcagg tgataacgca gcgcttccca gacagacctg
    36901 cctactgggt agagaggatt taaactgctt gccggatctt ggaaaaccaa accgatttct
    36961 gtgccgcgta acttttgcca ttgctcacgt ttgtcgaaag aaaacgtttt ttgttgataa
    37021 atcatttctc cctgtactat agcctcagga ggcaataaac cggtcagggc taaggctgtc
    37081 atacttttgc cactgcctga ttcaccaaca atgcccagta cttctcctgc tgtcactgaa
    37141 aatgtactcc ctttgacaac ccacagtatt tccccctgtt ctggaaaacc aacttttagt
    37201 tctctaattt caagcacttt tgttcctccg tgtactgcga tcaataccca acgcttgacg
    37261 taaaccttca ccagctaaat ggaaactaaa tacagtcaga aaaataaaaa gaccggggaa
    37321 aaatgcatac caccaagcgt aacgtaagta agcttgggca tcacgtaaca tattacccca
    37381 agtcggcatt ggcagctgta cacccagccc cagatagctt aaaccggcct ctgtcattac
    37441 ggctgcggga acacaaaggg ttgccgccac cagtaccgga ccgagggcat taggcaaaag
    37501 atgctgaaga ataatccgtc tgtttgtggc tcctaaaaga cgagccgaag caacaaaatc
    37561 actttgccgc agacgcagca cttctcccct tactaaccga gctacgcttg gccagcctgt
    37621 acagccgatg accaaaatta ccagccagat ggcaggttcg aagatagcca ccagcgtcag
    37681 cactaaaagt aaggaaggaa acactagaac aatatcagca aaacgcataa ttaacatatc
    37741 tattagcccg ccataaaaac ttgccatcaa accaagcagt gtgccgatcc cgactgaaag
    37801 aaaagttgct aacaagccca cagttaagga aattcgtgct ccgtaaagta cccgactaaa
    37861 gatatcacgg cctaagcgat ctgtaccaaa aagatgctgt tgactaggcg cttgtaacca
    37921 attatccaca tcagtttgat aaggactatg ggtagcaagc aggggggcca aaatagcgat
    37981 catgacaagg ccagtaatca gagtaaagcc aaaaaataat gaagaattat tcagcagccg
    38041 cattgattaa cctcctcccg cctatcttgt tctaggatct atcagcaaat ataaggcatc
    38101 agcaaggaaa tttgccattg ctaccagtga ggctgtatac aagttaaccg tcataatcac
    38161 gggatagtca cgtgccatcg tagaagtaat aatccagcgt ccgatcccag gccagccaaa
    38221 aacatattca gcgaccatcg caccggtaaa aagtctgggt aaagaaagag cggctatcgt
    38281 aatgatggga atagcagcat tgggtaaaac atgccgatat agtatttctt tttcttgcag
    38341 accgcgtgac ctgacggcac ggatatagtc ctgatttaaa acatctaaaa tagcactgcg
    38401 tacatagcgt acataatgcg caaaattacc agccaccatc acaaacaaag gcatgagcat
    38461 atgccgtaga atatccaata aactgccgtc acctggcgtg cgcagcccaa ccgagggcaa
    38521 aaggggaaaa cgataagcaa ataatataat cagcacaatg ccaaaccaaa aattgggcac
    38581 ggaaatacct gctaaagaaa gcgtacagac aaagcgatca agccatgagt tttgtcgtgt
    38641 agcagcataa atacctataa ctatagccaa aaaaagagcc agacagaaag cagtaaccgt
    38701 taatagcagg gtggaaggca aacgttctgc aatcagctcc acgacaggtc ggttatatag
    38761 taacgaatcg ccaaaatcac ctcgaaaaac acggcttaac cagctaagat actgtgataa
    38821 taatggctga tccaattgtc ttaattgcag ccagcgttcc accgcagtcg ggtcaaggcg
    38881 taattccgga ttggcctgca ggggatgccc cggggctgca tgcatagcta aaaaggtagc
    38941 cgcagataca ccaaataaga caagcaatgt ttgagcaaag cggcgtagta aggataaaat
    39001 caaggggtct cctcctcact ggccaatctc cattcatgaa cattaaaaaa aagtgaatca
    39061 gggtgtggct taaaattttc cagcctagca ttggtagcat gcactgcttg acgtacatac
    39121 aaccaaatcg ccggcacatc cttaatgatt agttcttctg cttgacgata caatagtgtg
    39181 cgttgactgt gctcacttgt ggtcaccgct tctgctgtaa gttgatctac ttcctcattg
    39241 tgataagcta aactattgcc aggactttgt gaatgccaat gcacggacaa atctggatca
    39301 gcttccacgc cgcgacctaa aatggccaaa tcataatcac cttcattgac ggcagctaaa
    39361 aaggcctccc attctaaaag ctctattttt atctccatac caatactagc agctttttct
    39421 ttgagcatta aggccacatt ttcacgcact gtattgccta cattatagat taaagttagt
    39481 tttagttctg cgtaacctgc ttctaccagt aaacggcggg cagctgccag atcaactttt
    39541 tctgctttgc gcgtttcgtt ataggcaaaa tgcatgggca gcagtggtcc ataaagtggt
    39601 tcaccataac ctaacaataa atttttgatc acttcatccc ggtctaaaag catagctaaa
    39661 acctgtctca cctttttatc agccagtgct gaatctgctt gctgggagtt tataactaaa
    39721 tagtcataaa ccagacgggg gttagtatgg atggcaatgt cgggattttg cttcaatgtt
    39781 ttgatatcct ctaccctgac cgccgtagga ataatgtcta gtttaccagc caacaattcg
    39841 atcagctgcg cttcctgatt aggaataaaa cgatagtaaa tattttcaat atctggctgg
    39901 cctaaaaaat aattagggtt agcccgtaca tgcacatatt cggcacgctg ccagtccgct
    39961 aaaagatagg gacctgcgct gactgtcaat tcttgtgaat aggcatctgc ttccaggtca
    40021 gccaccgcta cctcctgcaa aagatgtgca ggtaaagggg aaaaggtgag catggctaag
    40081 aagggggcgt aaggttcttt taaacgaatc tgtacttttt tttcatcaat aatcgtcaca
    40141 ccggacagct ctcctgtcgc ttgtggcgag cgatgttcct gcttatatcc ttcagcaccg
    40201 gcgatatagc gaagcggtaa aaagagccaa cctgtataat cagggtgaca aatcacccgt
    40261 aaagtaaaag caatatcttc tgtagtcagc ggtttcccgt ctgaccaaca agcgtcttca
    40321 tgcaaagtga aaatataagt caatttatcc tccagcagct gccaggaagt aaataaacgt
    40381 ggttgtggcg ccagcgtctt aggatctgtt actaacagct gtggatgcaa taaacctgct
    40441 atttcagcag aggcactatc acgtaagaat acaggattca gagttaaagg accggtcagt
    40501 gctgctacat gtaaagattg cccgcttgta gcagtttctt gctgacaacc tgtacaaaag
    40561 agcatgaaca gtaaaatgaa gattaccagg atccgccgca ttggatcacc ccctgtttag
    40621 aagctggata gcttgtcaac agctatatcg taaatatatg ttaacatcac cacaaaaaga
    40681 actggggtca ggcttgaatt attcacttgc tcattttccc acaaagattt tcccaatcga
    40741 gtaggagact acccattaat tgaatagccg tcctctcaca ccaccgtacg taccgttcgg
    40801 tatacggcgg ttcaatagaa ttagtggact agtgaatatc tttcactgat tgacagaagt
    40861 cccagcttct taaggtattc attggtgagg gttttcgaaa ggatagggct accggcagta
    40921 cgccagtagc tctttctcgt gttagcgtgt tcccaagcct tgtaacctga tacgcctagt
    40981 gcgacaaggt tgttgtgctt ggttttaatt ttcttccatt gcttccagta acacattcgt
    41041 atccttctgc gaagccactt gtccaatgat ttagcgagtc ctctcatgtc tgcaatgcca
    41101 aagtaattga cccatccgac gataatttgt cttaacttaa ccatcctctg gtacatactc
    41161 atggcattac ttctacccgt gatttcctta agtctttcct taagtttagc aacagattta
    41221 ggatgaactc gtatcccgat tccgcctttt ttagggtaaa atgagaatcc aagaaatttg
    41281 agcttccatg gtctatccac atcacttttc tctttattga ctttgagctt tagctcaccc
    41341 tcaataaagc gagtgatact tgccatcact ctatttgccg cttttctgct tctgacgtag
    41401 atattgc
//
LOCUS       DASRMS010000011        34449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_874293, whole genome shotgun sequence.
ACCESSION   DASRMS010000011 DASRMS010000000
VERSION     DASRMS010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 34449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34449
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874293"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VFC74_00665"
     CDS             complement(<1..727)
                     /locus_tag="VFC74_00665"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZK23881.1"
                     /translation="MQEKIKRLLTPEGVRDLLPDIARQKRMIEDKIQGLFSRWGYQEV
                     STPAFEYSANFVGEMKDGLDNKLYRLLDERGRTLVLRPDFTIPLARVAATHLTQAPKP
                     LRLCYGGSIYRYTTGQQGKQRELTQAGVELLGSPSAASDAEVIALAAAVLQAAGLTEF
                     TLCLGHSGFLENLLSAYQVDTAERALIKHLFNKKDFVALKNQVSQLPLAAWQKENILR
                     VPSLRGGREVLTAARELLPEPQAA"
     gene            complement(801..2174)
                     /locus_tag="VFC74_00670"
     CDS             complement(801..2174)
                     /locus_tag="VFC74_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK23882.1"
                     /translation="MAARSGEEQGVLLVNPNWQQLEAPFEHLGLGYLAACLRQAGIET
                     LILDLPLTGGDLSTVVQKVQQEKYALVGFSLTFQESAAETLQFITKLKAQTTVKIVVG
                     GIFPTFAAREIMSLCPAIDFVVKGEGELTLVELAQAILTNSSLQGVTGLLGRSGSELW
                     ETEDRPNIKDLDLLPEPARDTLPQVLLQTGYASILSSRGCYGRCSFCSVDAFFARFGS
                     KYRQRSAESVLAELEKLQTAYGVTNFAFIDANFIGGKGRAQARARLLAEAIISKGWQI
                     KFSIECRVNDVEPKLFALLKKAGLCKVFLGVESGSQAVLDRFQKDVTVAENLEALKIL
                     SELDIFVAMGFIMFDDMTSFDDLTANLQFLQQVKTVTGRPYLAKVDPASKVLPLAGTK
                     IEARLKAEQRYQGNTLNSSYKIADPLVEKVYQIIRVGAKVSQKFRTLRGRSAYHEFDW
                     QTGKIKD"
     gene            complement(2197..3321)
                     /gene="bshA"
                     /locus_tag="VFC74_00675"
     CDS             complement(2197..3321)
                     /gene="bshA"
                     /locus_tag="VFC74_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HZK23883.1"
                     /translation="MKIGMICYPTHGGSGVVATELGKELAKRGHVVHFISYQQPFRLD
                     SFQQNVFYHEVEMLAYPLFKYPPYTLALINTIAELAEREQLDILHAHYAIPHSFCAHL
                     AREMMSRQDLRVVTTLHGTDITLVGSDPSYHRITRYSIDKSDGLTAVSQSLRAETEQT
                     FAPAQEIRTIYNFIDPKRVFRLPGARRVVGCPEESCKVLVHISNFRPVKRLQDVVQIF
                     AHVRRVMPARLLLVGDGVERRTTQALVDELGLQQDVRFLGTQDNVIPILSAADLFLLP
                     SEKESFGLGALEAMACGVPVVASNTGGIPEVVVHNETGYLAAVGDVEAMSNYALEILT
                     NPAKMQQMREAARRRVEDVFDSELIVPQYEEFYREIINSK"
     gene            complement(3325..4059)
                     /gene="bshB1"
                     /locus_tag="VFC74_00680"
     CDS             complement(3325..4059)
                     /gene="bshB1"
                     /locus_tag="VFC74_00680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HZK23884.1"
                     /translation="MQLDILAIGPHPDDVEIGIGGTLIKHVQMGYKVGIVDLTAGESG
                     TNGTPEIRRQEALRAAEIMGVATRDCLGLPDARLAVNEEMLRPIIEIIRRYRPQVVLG
                     PYSKDRHPDHLRAHQLVREAAHVAGFWKYPADGEAHRPPVVLQYFLGGRAEPTLVVDI
                     SPYHELKMAAICAHASQFGIREDVELQTYLNDPEFLRFMRVRDQYMGSLIQVAYGEGI
                     VMDEQVAIGDLMTLQGRLRQTKKRER"
     gene            complement(4095..5714)
                     /gene="bshC"
                     /locus_tag="VFC74_00685"
     CDS             complement(4095..5714)
                     /gene="bshC"
                     /locus_tag="VFC74_00685"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HZK23885.1"
                     /translation="MLKSIKTLTPAGIYQDYLQGLPQATAFLGPHYRERKVFAETAAR
                     VSTAYQGDRAKLVALLLAYNRSIGGSAAAEVQLKKLTNPQSVVVVCGQQAGLLTGPLY
                     TIYKALAAVKLAAQLEGQLARPVVPLFWIASEDHDFLEANHCYLQNSEQQLQKLELPL
                     TPSGEPLGRLPLTAEAKETVIKELNEILPASEFKAELMQWLTAVCQLADTPVSWFARL
                     FSKLFNESGLVLFDPLLAEARQLAAPVLAKAVELRQEIEETLAEREAALVQHGYSLQV
                     QRAPDSTLLMRMDERRAALFFREGHYSTRDGSQTWKEAELLAAARKEPQSVSANVLLR
                     PLVQDTIFPTLATVLGPGETAYFAQALALYPLFGLQQPCLVPRPSVTVLEARLNRYLT
                     RYQVPETALLTDLDSYLQQVLQESSVVDLTAVFAQLKEQLAAAYQEAKSNLRQLNPQL
                     ATLAEKNLQHVYQQVAYLENKAQAEQKQQQHVTIRHFAALQEALTPQDKPQDRVLNIV
                     QYLAKYGPDWWQQLQEEFTAAPGHYLYQYEQ"
     gene            complement(5799..7085)
                     /gene="aroA"
                     /locus_tag="VFC74_00690"
     CDS             complement(5799..7085)
                     /gene="aroA"
                     /locus_tag="VFC74_00690"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HZK23886.1"
                     /translation="MLMTIAGQGALQGAVQVPGDKSISHRAAIIGALATGETRIEGFL
                     SAEDCLATLTCLRQLGVPITVSDTTVQIQGVGLTGLREPEGVLDCGNSGTTARLLLGV
                     LAAQQFSTVITGDDSLRQRPMGRVTEPLLLFGARISGGRQAQLLPLTCHPAAMRAGHY
                     RTPVASAQVKSALLLAGLFNSGETQISEPHQSRDHTERLFAYFGAGIKMQGTTVCLQG
                     PAQLTGRLVQVPGDLSAAAFFLVAASIVPGSEVVLHNVGINPSRAGILNVLQAMGGDL
                     QLFNQREVGGEPIADLRVRSADLHGVEISGALIPKLIDELPILAVAALFAKGRTTIKD
                     AQELRVKETDRLAAVAEEFSKLGAVITPTADGLVINGAQHLSGGTVHSRGDHRMVMSL
                     AIAALRAAEPVTIIGADAVAVSYPHFWQTLQGIQRN"
     gene            complement(7089..8186)
                     /locus_tag="VFC74_00695"
     CDS             complement(7089..8186)
                     /locus_tag="VFC74_00695"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HZK23887.1"
                     /translation="MLCLRRVAIIGVGMMGGSLGKALLKRNLAKEVVGFDADPAALKQ
                     AQEVRAITRCAATVAEAVAEAEVVVLAAPVLAVCQLLAEIAPHLHDDTIVTDLCSTKA
                     VVTEAAEQVLPAAVTFIGGHPMTGSEKEGVLAAEADLFENAIYILTGLPSPQLTEMQH
                     LVRRLGGLPVVFTPQQHDRQVAAISHLPHLAASVLVQTVASLNETTELLSLAGGGFRD
                     STRIALGSPQMWQEICLSNRDNLLAMLDLFAGELLQVRDFIACGNAEELLAHFAGAKQ
                     VRQQVPMRGKGLLPLLYNLFVFVPDQPGLIGEVASLLGEGGVNIAEIELLRVREEAGG
                     PLRLGFVEADARRLAAKILTAAGLRVELQEE"
     gene            complement(8186..9214)
                     /gene="aroF"
                     /locus_tag="VFC74_00700"
     CDS             complement(8186..9214)
                     /gene="aroF"
                     /locus_tag="VFC74_00700"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZK23888.1"
                     /translation="MVIVMQSGVKTEEIQQVVARLEEVGLGVHLSEGKFRTIIGAIGD
                     ETLVRQMSLEALPFVEKVLPITQPFKLVSREFQQENSVFTVGDCTIGDQQVVLMAGPC
                     AVESEEQLLAAAEGVKAAGAQILRGGAFKPRTSPYAFQGLEKEGLKLLARVREQTGLP
                     IVTEVMDQHTVGLVAEYADILQVGTRNMQNFHMLRELGKLDKPILLKRGLSATIEEWL
                     MAAEYIMSSGNHQVILCERGIRTFETYTRNTLDLSAVPVVKHLTHLPVIVDPSHGTGS
                     WRWVPSMSRAAVAAGADGVMIEVHPHPDQALCDGAQSLTVEKFTTLTKELEQVAAVLG
                     KHITQVPA"
     misc_binding    complement(9397..9643)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(9697..10692)
                     /locus_tag="VFC74_00705"
     CDS             complement(9697..10692)
                     /locus_tag="VFC74_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZK23889.1"
                     /translation="MKSKGKGPLTILAVVLLLLLQNGYAAAQPSKVVADAPTIAVESA
                     FLPTYLQQLQEQLADWAAATALRHTIARGETLNMIAKEYGISLEQLINDNNINNPNII
                     YVGQVLTFSEEKDNSQDSEALLTHTLQRGETVWELAKHYNAQVSAILTANKITDPTKL
                     EVGRELVIPQGSKPSGSKNTTRVLAARGSDSTSQSSGSNERRVISRNGRNISFTQGYK
                     VSATAYCAGTAASGCPIDSKGRSVCTGSYNDGITASGRRAVAGDGSEANPHLVAVDPK
                     TIPLGSRLYIDDYGFAVAADTGGAIKGKRLDLLLPSHQTALKFGRRQLQIYLLPQ"
     gene            complement(10980..12236)
                     /gene="dapG"
                     /locus_tag="VFC74_00710"
     CDS             complement(10980..12236)
                     /gene="dapG"
                     /locus_tag="VFC74_00710"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZK23890.1"
                     /translation="MQIVVQKFGGTSVANPALRKEVVKRVRDAQAAGFKPVVVVSAMG
                     RLGEPYATDTLKELMLKTSEQTEPSLRDMDLIMACGEIISAVVMSSTLCANGIPSLAL
                     TGGQAGLVTDGNYGSAQVDEFQPERLMAHLRNDEVVVVAGFQGETAEGEINTLGRGGS
                     DTSAVILGAGLGAQRVEIHTDVNGIMTADPRLVANARIIDHLTYNEVCQLAYEGAKVI
                     HPRAVEVAMQYNVPLIVKHLSEDEAGTVISNEVIASREAHFHKVSRVITGIAHIPGLV
                     QIFVEMATEDAEAELELFDQLAKAHISLDMISIFPERKSFTIVEADLANTESILESLG
                     LTYRVVNNCAKVAVVGLGMRNLPGVMASVVKALRDARIRILQTGDSNITISLLINEDD
                     LSEALCTLHEHFHLSSPPSEDENVLA"
     gene            complement(12248..13285)
                     /locus_tag="VFC74_00715"
     CDS             complement(12248..13285)
                     /locus_tag="VFC74_00715"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HZK23891.1"
                     /translation="MKKYNVAVVGATGMVGQSLVQVLEERNFPVGELKLLASARSVDT
                     QVEVMGRTFTVEEACPEAFDGVDVAFFSAGEAVSLALAPEAVRRGTVVIDNSNAFRMD
                     EGLPLVVPEVNPEQLANHQGIIANPNCSTIQLVVALQPLQKAAGLKRVVVASYQAVSG
                     AGKEAVDELTAQTQAVLNGAEAMPRERFPHANAKVQHQMAFNMVPQIDAFLEDGYTKE
                     EMKIIKESQKIMGLPDLKITSTTVRVPVIYGHSEAVSVEFERPITPEEVREVLRNAPG
                     IVVMDDPAELLYPMPQDLAGRDEVFVGRIRVDYSVPYGVNIWVVADNIRKGAATNSIQ
                     IAETLIARGQL"
     gene            complement(13366..14703)
                     /gene="lysA"
                     /locus_tag="VFC74_00720"
     CDS             complement(13366..14703)
                     /gene="lysA"
                     /locus_tag="VFC74_00720"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HZK23892.1"
                     /translation="MFLSGTMLINEQGHLEIGGCDTVALAKQFGTPLYVMDEELIRHN
                     CGIYRDTFSRLYPHSQVAYAGKAFLTTAMCRLLDEEGLALDVVSGGEIFTALTAGFPA
                     AKMIYHGNNKTPAEIKQALAVGVGRFVVDSISELELLESIAAAAGKKANIYFRIKPGI
                     EAHTHHYIQTGQLDSKFGLGLADGQALAAVKMALQMKHLVLQGLHCHIGSQIFDLKPF
                     HLAAQVMMDFMAEINNETGYVIKELDLGGGVGIRYLQSDAPHPVSDFLELITTTVKEK
                     AAAHNLSLPKLLVEPGRSIVGEAGTTLYTVGTIKDIPGIRRYAAVDGGMMDNLRTALY
                     EAKYEALVANRALEEAEETVSIAGKACESGDMLIWDIKLPKLARGDLLAVLSTGAYHY
                     SMANNYNRFTRPPVVFVYRGQADLVVARESYEDLVRNDLMPARMQRAAHKIAE"
     regulatory      complement(14790..14980)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00168"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Lysine riboswitch is most abundant in Firmicutes
                     and Gammaproteobacteria where they are found upstream of a
                     number of genes involved in lysine biosynthesis, transport
                     and catabolism; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="lysine"
                     /db_xref="RFAM:RF00168"
     gene            complement(15065..16456)
                     /locus_tag="VFC74_00725"
     CDS             complement(15065..16456)
                     /locus_tag="VFC74_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HZK23893.1"
                     /translation="MRLLVKYLLYLFTSLILLIMLALPTTADSPQHEPPAAVPIETTV
                     SPAMVRLGYAALRGHFTAFRSVAETERLLGSTTTALSQQQFYQILARTPNEQLTAAPR
                     EYFLGIEGEILPLEAWAQLGIVEVPGTRLFKAAEQVSWQAAQQDLARAVFPQLRLQNE
                     PAVVSPQQVYSYQQLEEDLQRLVTSYPELLKLQVIGESVEGRAIYALRLGEGEEEIIL
                     DAAIHASEWLTAPLVMKLAEEYAHHAHYQTDFASHDVAALLKEVSFWFIPMVNPDGLN
                     LVQAGPQAITNGALVEQMLAASEEIESFKAWKANIRGVDLNRQFPISWEVLENVQEEP
                     APSHFKGFTPLSEPEARALSEFALARQPLLTLSFHQQGEWLFWYHKQKGAQFDRDQRL
                     AAALAALTGYRYQYYDDNGGKYMDFVIQELGVPAITVEVGTVVGDLQQWPRIWQQVRT
                     LPLVAAKLRLQDQ"
     gene            complement(16556..17122)
                     /locus_tag="VFC74_00730"
     CDS             complement(16556..17122)
                     /locus_tag="VFC74_00730"
                     /inference="COORDINATES: protein motif:HMM:NF014573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HZK23894.1"
                     /translation="MLVLGIAGSPRRKGNTEILLDEALASAKQAGAEVEKVVLSTLDF
                     SPCISCGECEKTGECIFKDDMQDVYKKIGAADVMIFASPNYFYNVSAWAKKAIDRSQA
                     LWARNHILKDPRFRQGKKGYFISVAATKGARLFKGSIWTMQYFFEAVGYKMAGRLLVR
                     AVDGKGDILKEPQYLEAARKLGLEAASE"
     gene            complement(17116..17562)
                     /locus_tag="VFC74_00735"
     CDS             complement(17116..17562)
                     /locus_tag="VFC74_00735"
                     /inference="COORDINATES: protein motif:HMM:NF016160.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR reducing system RseC family protein"
                     /protein_id="HZK23895.1"
                     /translation="MEQVGLVVEVKDDKAIVAVQRHNVCTKCGGCGVAVSGRGETHLE
                     ALNKVNANLGQTVKIYSDTSHILTASFMVYIVPLLALLLGLFIGQQLDGTAGGFRFDI
                     LLGAAFLLLSYLLVRGYDRKVASRQLEVSVIEILPEPYTGPEDEQC"
     gene            complement(17819..18694)
                     /gene="ccsB"
                     /locus_tag="VFC74_00740"
     CDS             complement(17819..18694)
                     /gene="ccsB"
                     /locus_tag="VFC74_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR03144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcsB"
                     /protein_id="HZK23896.1"
                     /translation="MSSYIQVSPMGLFWENTFFNLALVTYLFATLCYVAHLLGKKQLG
                     RFASYILRVSFLLHTGFIVTRALNVQRVPFVGSFEFGNMFVWATVLVYLWTEWRMKEQ
                     YYAVGAFVTPLAMLYLGYLTVVPRLFPAIRISRAHTQLAPVLQNNWLTVHVSASIFGY
                     AGFTLAFAAAIMWLLKVNLKADHLITRNLPQSKTLEEYMYRGAAFGFLFQSVMIITGA
                     IWADISWGRYWGWDPKEMWALITWFVYAVYLHARFTRGWTGLRTIALVMIGWVCMVFT
                     WVGVAWLLTGLHSFG"
     gene            complement(18687..20000)
                     /locus_tag="VFC74_00745"
     CDS             complement(18687..20000)
                     /locus_tag="VFC74_00745"
                     /inference="COORDINATES: protein motif:HMM:NF016995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein ResB"
                     /protein_id="HZK23897.1"
                     /translation="MTKETPKTAVKSPLQQLLNFLSSMQLGIILLLLLAVISVFATLK
                     ENETAITSIYHSWWYLGMMAFTALNLLLCTARRIKPVTKLAFHPHKLTSPAALRQMPH
                     HYELKMEKVTAEAALAQAEAAFKKNGLQVEVTAGQQGRVLFAERGKIGYFGSLVTHLS
                     LLVILLGAMYGSLTGFEARNGGLAGDHFHVEQGDFRVDIKDVKMEQEADPTVRPRVFS
                     ELVITRDDQPLSEGTVAINQPLRFNGNTIYHTTFRYVSDVVATDLATGKEQQLKLWDI
                     DQLQLDEEGKVILHVMQFFPNFSMHGGGVPYSKNYLPENPVLAGLLIENNKAVRNVFL
                     ILNEPEVIKTSTGEIELLMTGFENAAVFTITRNLGRPILFTGAVLLILGLYLSFFTFP
                     RRFWAVFAQESNSLIIGGRSRNRLLLEQELEQINQEISKGGEARV"
     gene            complement(20046..20432)
                     /locus_tag="VFC74_00750"
     CDS             complement(20046..20432)
                     /locus_tag="VFC74_00750"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HZK23898.1"
                     /translation="MKRYDKIVLLILLVVFGVGIFMTARNALSTYVTFAEAKATERAV
                     QVKGLAVAGTLEELADGEYVFQMEDQNGEVARVVASGQAPDNLFAAESVVAKGKYSGA
                     DFLADNILVKCPSKYEAEEHPDEIEK"
     gene            complement(20419..20538)
                     /locus_tag="VFC74_00755"
     CDS             complement(20419..20538)
                     /locus_tag="VFC74_00755"
                     /inference="COORDINATES: protein motif:HMM:TIGR04391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CcmD family protein"
                     /protein_id="HZK23899.1"
                     /translation="MAEGLSYVLAVTLITWLGLFFYLLRVDLKVKEAERREEI"
     gene            complement(20540..21196)
                     /gene="ccsA"
                     /locus_tag="VFC74_00760"
     CDS             complement(20540..21196)
                     /gene="ccsA"
                     /locus_tag="VFC74_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HZK23900.1"
                     /translation="MFWQIILGIWMVGVIIASFLYMPATQGLGETGRILIYHVPSAWI
                     AVLAYLMAMVNSLGYLRQGDLRWDRQAQVNAELGTIFCILATVTGAIWSRGAWGQYWN
                     WDPRQTSITVLLMIYAAYFVLRSAIPDADRRARLAAVYAILAFLTVPFLVFIVPRVYV
                     SLHPDLINLENRSFDMDRKMLVVFLSSLLGFTGLYAWMYKVQMRLENLLYAIDMKRGE
                     "
     gene            complement(21246..21926)
                     /locus_tag="VFC74_00765"
     CDS             complement(21246..21926)
                     /locus_tag="VFC74_00765"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HZK23901.1"
                     /translation="MIKPLRWLKQGLAIWRKDLRIEFRTRYTYSALVMFAITTLVTVS
                     FSIGGFVTDTDITAALLWIILFFSAMAGLARTFVAEEETGTATALKMAAQPEPVLLGK
                     YLFNVTILLSLTVLVVPLFIMMLNLSIALPLAFVAAVVLGSLGLAGASTILAAIVAKA
                     GAKSSLMTVLAFPVLLPLLFAAISATRAALGGGLAADVTADLLMLFFYNGVALAASLL
                     LFEYVWNE"
     gene            complement(21901..22518)
                     /locus_tag="VFC74_00770"
     CDS             complement(21901..22518)
                     /locus_tag="VFC74_00770"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK23902.1"
                     /translation="MLNQVVVKNLSKAYQRRKLFTDVSLTIPDNSVLVVLGPNGAGKT
                     TFLRILCGLTPATSGQVDIMINGQILTAGQRRAQLGLVTPELELYGELSAVENLEFFA
                     GVRELPFSRQQARELLAFVGLKGRGHDFLKTYSSGMKQRIKYAYALLHQPHILVLDEP
                     TSNLDEAGVALVDTIIRQQKERGIVIMATNEPGEVAYGDQTFTLA"
     gene            complement(22720..24540)
                     /gene="asnB"
                     /locus_tag="VFC74_00775"
     CDS             complement(22720..24540)
                     /gene="asnB"
                     /locus_tag="VFC74_00775"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HZK23903.1"
                     /translation="MSGFIGLWNKTSSRLERDLDAVTAAMRHRGPDEADSLYLGSVAL
                     GSRRLKIMDLSARGRQPMLNEDGTLALIFDGKIYNYRELREGLLLRGHVFRSDSDYEV
                     VLHLYEEEGEACVTKLRGMFAFCLYDQRNKQLFGARDHFGIKPLYYTETADYFALASE
                     AKAFTKLPAFRVQPNAAAIPHYLTFQYVPEPETMFAGVYKIPPAHSFKWREGKLSLTR
                     YWQIAFAPQDRPLEELLEGTQAIMRESVRLHTQAEVPWGAFLSGGIDSTVIVALLREL
                     GPVSTYSVGYEEEGYSELSDAAATARYLETNHHEYVIGAEEYWRNLPQLVWHFDDPVA
                     DPAGIALYYVARLASKDIKVALSGEGADELFGGYGIYKEPGDLKLAQHLPQPLLAAAA
                     KLWPGFLPGKNFLRRAATPLENRFFGNACIFNEAEKQKILKQKRFAPATVITAPLYRQ
                     MAAFDDVTKMQYVDLHTWAVGDILVKADKMTMANSLELRVPFLDQQVFEYAAAIPSAY
                     KIKGGMTKYVLRQAFAELLPPEVIKRPKRGFPVPTRVWLRGPLAKEVAELLNTPLLAQ
                     YFNRAYIRTLLAEHREGQADHSRKIWVLVILALWLQQFAN"
     gene            24693..24842
                     /locus_tag="VFC74_00780"
     CDS             24693..24842
                     /locus_tag="VFC74_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23904.1"
                     /translation="MCHRETNYEFAIMPDNEISKIRDVEKTLSQNGEVILLAYKRSDQ
                     NVMDD"
     gene            complement(24947..25987)
                     /locus_tag="VFC74_00785"
     CDS             complement(24947..25987)
                     /locus_tag="VFC74_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HZK23905.1"
                     /translation="MTEKQGFLARKNIVFSLERYFIDALGAMALGLFSSLIVGLILAT
                     LGEQLTALIGANAIFTFLINSGKTAQSMMGPAIGIAVAYGLKAPPLVLFASVITGAAG
                     ASLGGPAGSFVATVFGAEFGKAVSKETKVDIIVAPAVTILAGLLAAAVVGPWVNNFMQ
                     SFGQLIMRATELQPIPMGIIVSVLMGLALTAPISSAALAIMMNLSGLAAGAATVGCSA
                     QMIGFAVASYRENGWGGLIAQGVGTSMLQVPNIVRNPWIVVPPTLASALIGPLATTIL
                     KMYNNPAGAGMGTSGFVGQFGTATVMGFTMPVLFKIILLHFLLPAILTLFFSEWLRKS
                     GRIKPGDYELKL"
     gene            complement(26014..27063)
                     /gene="selD"
                     /locus_tag="VFC74_00790"
     CDS             complement(26014..27063)
                     /gene="selD"
                     /locus_tag="VFC74_00790"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(27013..27015),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HZK23906.1"
                     /translation="MTNEQPRLTTLTKSGGUAAKMPPGALAQVLCQLPKMNDENLLIG
                     CDSFADAGVYRLREDIALVQTLDFFTPVVDDPYVFGQIAATNALSDVYAMGAQPRLVM
                     NIVCYPAKTLGTAPLAEILKGGADKVLEAGALLVGGHSVEDNEPKYGLSVTGFVHPQQ
                     LITNSAAQIGDALVLTKPLGSGLIMTAVKAEMAGSGELEAAVQTMITLNKEAAAVMTE
                     IGVSAATDITGFGLLGHARELALASGVAIELSFGRIPLLPGALRAAEMGLVPGGAYAN
                     REYVADGLQVVGPAVGDRELDVLCDPQTSGGLLISVPAAKEAALIQTLAARDVPAAVI
                     GRVTAGAAGQIKVLA"
     gene            complement(27056..28990)
                     /gene="selB"
                     /locus_tag="VFC74_00795"
     CDS             complement(27056..28990)
                     /gene="selB"
                     /locus_tag="VFC74_00795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HZK23907.1"
                     /translation="MSHVIIGTAGHVDHGKTALIKALTGEDTDRLHEEKERGISIELG
                     FAPFKLPSERLAGVIDVPGHERFIHNMLAGIGGIDLVLLVVDVTEGVMPQTREHVEIM
                     DLLHIPRGLVVLAKIDLVEDVDWLDLVEEEVREALAGTFLAQAPYYRVSAHTRQGLPE
                     LLQAIDELTADIENRDEKAPLRLPVDRVFTVPGFGTIATGTLLAGTIHLGSTVQVLPL
                     GRTARVRQIQVHGAQVESAAAGQRTAVNLSGLEKEALPRGSVIAALDSLEATYLIDAK
                     LKLLASAPRAMKNLTRVHLYLGTGRAVGRVALLDRDELKQGEEAPVQLRLERKLVAQR
                     HDRFIIRSFSPMTTIGGGLVLDARPSKHKRFKNDILHKLKELEKGDPAAPILQRIRRE
                     GAVAHSLLLKESGLAPEVLQTELSKLLTDGKIKQVGELYVDALAAQEWSERLEQVLTE
                     YHQAYHLAVGMSRAALKKTLPQQVTPKLYDWLLTTLVTAERLRLYEDLVALAAHHPQP
                     SEKETKQITALTELIKSGGFMPPTSKEAAAKLVLTTAQIETYLSYIAQQGLLVRLDEQ
                     LALHQEFFQQAKEVLCQHFRAHQTLTTGEFREKLNTTRKYVIPLLEKFDHLKWTRRLR
                     DERVAWRLDITDCGEDGKDD"
     gene            complement(28978..30378)
                     /gene="selA"
                     /locus_tag="VFC74_00800"
     CDS             complement(28978..30378)
                     /gene="selA"
                     /locus_tag="VFC74_00800"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HZK23908.1"
                     /translation="MVENWRNEYLRSLPAVDAVLMSYQSVFTDEHYAHTLLVQVVQEV
                     LAQLRQQILAAPAAEKLVTLNLTPQAVASVVQARLESLKQPSLRRVINATGTVLHTNL
                     GRAPLAQAATTAITEAAHYCNLELELQSGKRGSRHVHVEKLLQRLTGAEAACVVNNNA
                     AAVFVALNTLAAGKQVIVSRGELVEIGGSFRIPEVMAASGAELVEVGTTNKTHLRDYR
                     NAVGEETALLLKVHTSNYQVVGFTAAVGVAALVELAQEVNLPVMEDLGSGMLLDLSAF
                     GLAKEPLITERIAAGVDVLTLSGDKLLGGPQAGIIVGKRKYIEQIKRNQLLRALRPDK
                     FTLAALEATLQIYVTGNPLQEVPVLAKLSITAAALRQRAAKLAAAITAQAQARLIVEV
                     REDVSYVGGGAMPTTYLPTYVIAVRHRQVPLHQWVAQLRLGTPALVGRVQDDWLLLDP
                     RTIAESEEEEVVQCLT"
     gene            complement(30488..31852)
                     /locus_tag="VFC74_00805"
     CDS             complement(30488..31852)
                     /locus_tag="VFC74_00805"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK23909.1"
                     /translation="MKNSLSRRFTIAMIILAVGLILVFALVTQLALGWNFERYLDNVQ
                     EQQNRLILGTLADLYSESTSWQSIRLATLYVGNTTGAQIRVFDAHGDLIVDSLTGMMQ
                     RKHGRHWQNAQQAKGNTYSYPLVAGQTTVGTVEITHLGQEGLWTTEALVFQQTVQQSG
                     LITGLLAAVAAIILAGLLARRLTARLQSLTETADKWGSGQIEARVEVDGEDELAVLGT
                     TMNRMAAKIADQNALRRKLTGDISHELRTPLTTIQSYLEAFRDDVLPPDEENIAAVLE
                     ESTRLGSLVNDLQELANADFHTRNVTLKVLDFNELLQTEVERLKPLLQRKELDLQLEL
                     PAQTEYVRADETLLGRVLNNLLNNAYKYTPLGGVIKVTLFQQAAEVGLVVKDTGIGIA
                     AEHLPYLFERFYRVDPSRARTTGGSGIGLAIVKELVEAMGGRVTVESEPGEGSSFSVY
                     LPRP"
     gene            complement(31855..32547)
                     /locus_tag="VFC74_00810"
     CDS             complement(31855..32547)
                     /locus_tag="VFC74_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK23910.1"
                     /translation="MERILVVDDEQKITRVITAYLQKEGFAVTEAYDGAEALKLAQSG
                     DFALMVLDLMLPVFSGEEVTKRLRQTGNKIPIIMLTAKGGEEERILGLGLGADDYVVK
                     PFSPGELVARVLAVLRRVRSAPGVLADVLEYADGELVINTLCHQVSFRDQIVELTATE
                     YKLLVAMAKSPGRVFSRAELLEIAQGVYATGYDRSIDSHIKNLRHKLEPNANEPRFIR
                     TVYGVGYKFEGN"
     gene            complement(32678..33049)
                     /locus_tag="VFC74_00815"
     CDS             complement(32678..33049)
                     /locus_tag="VFC74_00815"
                     /inference="COORDINATES: protein motif:HMM:NF022378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2680 domain-containing protein"
                     /protein_id="HZK23911.1"
                     /translation="MKKGLLLALVVMLVLAAAVPAAMALTDKQKDELAKLYEQEHQLR
                     LQIVEKQAESGVLTKEDAKVMQERMTEQWQDHEKSMQEGNYSFGPQGRGGGRGGRRNG
                     NCGNCPYNQQGSAVTPEQSAL"
     gene            33206..>34449
                     /locus_tag="VFC74_00820"
     CDS             33206..>34449
                     /locus_tag="VFC74_00820"
                     /EC_number="1.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HZK23912.1"
                     /translation="MKQKKLKKALLIGAGAATGLTAAKLMVRGAINKTHDHALKILME
                     DKYDENMWELISAATRIGLQNVLETNLRATEGKAIARPMGSPKKFPSLDDLVFTFAQM
                     YIMPTPLEANIDTTVVIGKKAKKPFTVDFPIMIAPMAYGEALSKKAKIALAKGAALAG
                     TSTNTGEGPFLPEERKAAKYLIFQFNRGDWNKDPEIIRQCDAIEIQIGQGALGGVGHV
                     MPAEMIDKELREAFSFPKGKDAVAHSRQPGVESPEDFTRLVSRLKEQGEGIPIGVKMA
                     AGKHLEADLRILCEAGIDYIAMEGAEAATKSSAPILQDDFGVPMVFAISRAANWLKEN
                     GYSDKVTLIASGKMRTPGDVLKACALGADAVYMGATALFAVAHTQVLKPLPFEPPTQI
                     VWHNGKYAKQFSIKKGTKYLFQ"
BASE COUNT         9216 a         9168 c         7349 g         8716 t
ORIGIN      
        1 gcgcagcctg cggctccggt agtagttctc gggccgccgt taagacctcc cgcccaccac
       61 gcaagctagg tacacgtaaa atattttctt tctgccacgc ggctaagggc agttgactaa
      121 cctggttttt tagcgctaca aaatcctttt tattaaataa gtgtttgatc aaggcacgct
      181 cggcagtatc tacctgataa gcagaaagta agttttctaa aaagccacta tggccaagac
      241 acaaggtaaa ttctgttaaa cctgctgctt gtaatactgc tgccgccagc gcaatgacct
      301 cggcatcact ggcagcactt ggggagccca gcagctccac acccgcctgc gttagctcac
      361 gctgcttacc ctgctgccca gtcgtataac gataaatact gcctccatag catagtcgta
      421 aaggcttggg tgcctgcgtc aaatgggtgg cagctaccct ggcaagggga atagtaaaat
      481 caggccgcag cactaatgtc cgcccacgct catctaaaag acggtaaagt ttgttatcta
      541 aaccgtcttt catctcgccg acaaaattgg cactatattc aaaagccggt gtggatacct
      601 cttggtatcc ccaacggcta aataatccct gaattttgtc ttctatcatt cttttttgtc
      661 gtgcgatatc cggcagtaaa tctctcacac cctcaggtgt taagagacgc tttatttttt
      721 cctgcaactt gctcaccccc tctactttac cacgttaaag tgataacgta ataaagtata
      781 acacggggca aagctcactg tcaatcctta atctttcctg tttgccaatc aaattcatga
      841 taagcagaac gcccgcgtaa agtgcgaaac ttctggctaa cctttgctcc cacgcgtatg
      901 atttgataaa ctttttccac tagggggtcg gctattttat aagaagaatt taaagtatta
      961 ccttgatagc gctgctctgc tttcagccta gcttcgattt tagtccccgc caaaggtaaa
     1021 accttgctgg caggatcaac ttttgccaag tacggtcgac cggtaacagt ctttacttgc
     1081 tgcaaaaact gcagattggc agttaaatca tcaaagctgg tcatgtcatc aaacataata
     1141 aagcccatgg ccacaaaaat atctaactcg cttagtatct ttagtgcctc taggttttcg
     1201 gctacggtga catctttttg gaaacgatcc aatacagcct gtgaaccgga ctctacgcca
     1261 agaaaaactt tacacaagcc cgcttttttc agtaaggcaa agagctttgg ttccacatca
     1321 ttgacgcggc attcaatact aaatttaatc tgccagccct tgctgataat cgcctcggca
     1381 agtaaacgtg ctcgtgcttg ggcccgacct ttgccgccaa taaaattcgc atcaataaaa
     1441 gcaaaattag tcacaccata agcagtttgc agcttttcta gctctgctaa gactgactcg
     1501 gcactgcgct ggcgatattt gctgccgaaa cgggcaaaaa aagcatctac actgcaaaaa
     1561 ctacaccgcc cgtaacaacc tcgtgaggag aggatggagg cataacctgt ttgcagcagc
     1621 acttgcggca gcgtgtcacg cgccggttca ggcagaagat ctaaatcctt gatattggga
     1681 cgatcttctg tttcccacag ctcactgccg ctgcgcccca aaagtcctgt gactccttga
     1741 agcgaactat tagtaaggat ggcctgcgcc aattccacga gggtcagctc accctcacct
     1801 ttgaccacaa aatcaatggc cggacaaagg ctcataattt cccgggcggc aaatgtgggg
     1861 aaaattcctc ccacgactat cttgacggtt gtttgtgctt taagcttggt tataaactgc
     1921 agtgtttccg ccgcactttc ctgaaaggtg aggctaaaac caactaaagc atatttctcc
     1981 tgctgcactt tttgtactac tgttgacaaa tcaccgccag tcagcggcaa atcaagtatg
     2041 agtgtctcta tacctgcttg gcgcagacag gcggccaaat aacctaaccc taaatgctca
     2101 aatggagcct ccagctgctg ccaattggga ttgaccagca gtaccccctg ctcttccccg
     2161 cttcttgctg ccatttttct cccttcccca caccttttat ttactattga taatctctct
     2221 gtagaactct tcatattgag gcacaatcag ttcactgtca aacacatctt caacacggcg
     2281 ccgcgcggct tcacgcattt gctgcatttt tgctggattt gttaagatct ccagggcata
     2341 gttagacatg gcctctacat cacctactgc ggccagataa ccagtctcgt tatgcaccac
     2401 aacttcggga ataccgcctg tattgctggc taccaccggc acaccacatg ccatggcttc
     2461 caaagcgcct aaaccgaagc tctctttctc cgaggggagc aagaagagat ctgccgccga
     2521 taaaatagga attacattat cctgtgtgcc taaaaaacgg acatcttgct gcagacccag
     2581 ctcatctacc agcgcctgtg tagtgcggcg ctcgacccca tcacccacta atagcagacg
     2641 cgcgggcatc acacggcgca catgtgcaaa aatttgcacc acatcctgca atctttttac
     2701 cggacgaaaa ttagaaatat gtaccagcac tttacagctc tcttccggac aaccaacgac
     2761 acgacgagct ccaggcagac gaaaaacacg tttaggatca ataaagttgt agattgtccg
     2821 gatctcttgt gcaggggcaa aagtttgctc tgtctccgcc cgtaaactct gggaaactgc
     2881 tgttaagccg tcacttttgt caatactgta ccttgtgatg cggtgatagg agggatcact
     2941 tcccaccagt gtaatatctg taccgtgcaa tgtggttacc acccgcagat cctgcctgga
     3001 catcatttca cgggccaaat gagcacaaaa agaatgggga atggcataat gagcgtggag
     3061 aatatccagc tgctcccgtt cagccagttc tgcgatggta ttgatcaagg ctagtgtata
     3121 gggtggatat ttaaaaagcg gatacgccaa catttctact tcatgataaa aaacattctg
     3181 ctgaaagcta tccaaacgaa agggctgctg ataagaaata aaatgcacca catgaccgcg
     3241 ctttgccaat tccttcccca gttcagtggc tacgacaccg ctgccaccat gggtcgggta
     3301 gcaaatcatg ccaattttca tctgctatcg ctcccgcttt ttagtctggc ggagacgacc
     3361 ttgtagtgtc atcaagtcac ctatcgctac ttgttcatcc atgacgatac cttcgccata
     3421 agcaacctga attaaggagc ccatatactg gtcacgtacc cgcataaaac gcagaaattc
     3481 aggatcattt agataggttt gcagttctac atcttccctg atgccaaatt gactggcgtg
     3541 agcacaaatt gcggccattt taagttcgtg atatggtgaa atatctacca ccaaagtagg
     3601 ttctgctcgg ccaccaagaa aatactgcaa gactaccggt ggacgatgcg cctcaccgtc
     3661 ggcgggatat ttccaaaaac cagcgacatg agcagcttca cgcactagtt ggtgggcacg
     3721 caagtgatcc ggatgccgat ccttgctata gggccccaag accacttgag gtcggtagcg
     3781 gcggataatt tcgatgatcg gtcgcagcat ttcttcgtta actgccagcc gtgcatccgg
     3841 cagccctagg cagtcacgcg tggcaacacc cataatctcg gctgcccgta gggcttcctg
     3901 gcggcggatt tccggcgtgc cgttggtacc gctttcaccg gctgttaaat ccacaatacc
     3961 aactttataa cccatttgca catgtttaat cagcgtacca ccaatgccaa tctccacatc
     4021 atcagggtgg ggaccaatcg ctaaaatgtc aagttgcatc ttatcgcctt ccttctgcag
     4081 ttaattgcgg caagttactg ttcatactga taaaggtaat ggccgggtgc agcagtaaac
     4141 tcctcttgta attgctgcca ccagtctggc ccgtattttg ctaggtactg cacaatattt
     4201 aagactctat cctgcggttt gtcctgtggg gtcagagctt cctgcagtgc agcaaaatgg
     4261 cggatggtga cgtgctgctg ttgtttttgt tcagcttgcg ctttattttc taaatatgcc
     4321 acctgctgat aaacatgctg caggtttttt tctgctaatg tagccagttg gggatttagc
     4381 tgccgcaaat tgctttttgc ctcttgataa gctgctgcca gctgctcttt tagctgggca
     4441 aaaactgctg tcaaatcgac cacgctgctt tcttgcagca cctgctgcaa atagctgtcg
     4501 aggtcagtta gcagagcggt ttccggcact tgatagcgtg tcaggtaacg atttaagcgc
     4561 gcttccaaca ccgtcacgct tggtcggggc accagacacg gctgctgcag tccaaagagc
     4621 gggtaaagtg ccagggcctg cgcaaagtaa gctgtttctc ccgggcccaa caccgtagcc
     4681 aacgtaggaa agatagtatc ctgtaccaag ggacgcagta agacattggc acttactgac
     4741 tgaggttcct ttctggcagc agccagcagt tcagcttcct tccaggtttg gctgccgtca
     4801 cgggtgctgt agtggccctc gcggaaaaag agggcggcac gtcgctcgtc catcctcatt
     4861 aaaagggtgc tgtccggtgc acgttgtact tgtaaggaat agccgtgctg cactaaagct
     4921 gcttctcgtt cagctagtgt ttcttctatt tcctgccgca gctctaccgc tttagctaaa
     4981 acgggagcgg ctagctggcg tgcttctgcc aacagcggat caaacaaaac taagccgctc
     5041 tcattaaaca gcttgctgaa cagccgcgca aaccaactga ccggagtgtc tgctaactgg
     5101 cagacagcgg ttaaccactg catcagctcc gctttaaact cgctagctgg taaaatctca
     5161 ttcaattcct taattactgt ttctttggcc tcagctgtaa gcggcaagcg gcctagcggc
     5221 tcgccgctag gggttagcgg tagttctagc ttctgtaatt gctgctcgct attttgcaga
     5281 tagcaatgat tagcttctaa aaaatcatga tcttcactgg caatccaaaa cagcggcacg
     5341 actggtcgtg ctaactgtcc ttctagctgc gcagccagct tcacagcggc cagtgctttg
     5401 tagatagtat acagcggtcc cgttaataaa ccggcttgct gcccacagac cacaacgacg
     5461 gactgggggt ttgtcagttt cttcagctgc acttccgcag cagctgaacc gccaatactg
     5521 cggttgtacg ctaacaatag ggccacaagt ttagcacgat ctccttggta ggcggtagat
     5581 actcgtgctg ctgtttccgc aaaaacttta cgctcacgat aatgaggccc taaaaaggct
     5641 gtcgcctgcg gtaagccttg cagataatcc tgataaatac ctgcaggcgt tagagtttta
     5701 attgatttta acatttgccg ctcctgttca ctagattata gtgcattatt ccacaaaaaa
     5761 cctattttcc ctttcagtcc attgctctta gtccagtatt agtttctttg tatgccttgc
     5821 agcgtttgcc aaaagtgggg gtaagaaacc gccaccgcat cagcgccgat gatggtgaca
     5881 ggctctgctg cccgcagtgc agcaatcgct aatgacatga ccatgcgatg gtcaccgcgg
     5941 ctgtgcacag tgcctccgct taaatgctgc gcaccgttaa tcactagtcc atcagcagtt
     6001 ggggtaatca cagcccccag cttgctaaac tcttccgcca ctgcggctag gcgatctgtt
     6061 tctttgacac gtagttcctg cgcatcttta atcgtcgtgc gtcctttagc aaacaacgct
     6121 gctacggcta aaataggcag ctcatcaatt aatttaggaa ttaaggcacc gctaatctcg
     6181 acaccatgca agtcagcact tctgacacga agatcagcta ttggctcacc gccgacttca
     6241 cgttgattaa acagctgtag atcccccccc atcgcctgta agacgtttag aataccggca
     6301 cgggaagggt tgatccccac attatgtaaa actacctcgg agccaggcac aatactggct
     6361 gctaccaaga aaaaagcggc cgccgaaaga tcacccggca cctgcaccag gcggcctgtc
     6421 agctgcgccg gtccttgcag gcagacagtt gttccctgca tctttatccc tgccccaaaa
     6481 taagcaaata aacgttcagt atggtcacgg ctctggtgcg gctcgctgat ttgtgtctcg
     6541 ccactattaa ataaaccagc cagcagtaga gcggatttga cttgggcact agctacaggc
     6601 gtgcgatagt ggccggcacg catcgctgcg ggatggcagg ttaaaggcaa aagctgtgcc
     6661 tgcctgcccc cggaaatacg ggcaccaaac aagagcagag gttctgtcac tcgccccatt
     6721 ggacgctgtc gtagagaatc atcaccggtg atcacggtgg agaactgttg ggccgctaaa
     6781 accccgagta gtaagcgagc ggtagtaccc gaattaccgc agtctaacac accctcaggt
     6841 tcacgcagtc ctgttaaccc tacaccttgt atctgtaccg tcgtatctga caccgtaatg
     6901 ggcactccta gctgacgcaa acaagttagt gtggccagac aatcttcagc tgaaagaaat
     6961 ccttcgatcc tagtttcacc tgtcgccagg gcgcctatta tggcggcccg gtgcgaaata
     7021 gatttatccc ccggcacctg cactgcaccc tgtagagcac cttgccctgc aatcgtcatg
     7081 agcatcgttt attcctcctg cagttccacc cgcagccccg ctgcagttaa gatcttggcg
     7141 gctaaacggc gagcatcggc ctccacaaag cccagtctca gcggtccgcc agcttcttcc
     7201 cgtactcgca acaattcaat ctctgcaata ttaacgccgc cctcgcctaa caaactggca
     7261 acttcaccaa ttagccctgg ctgatcgggc acaaagacaa atagattata aagcagcggc
     7321 aacagccctt tgccccgcat aggtacttgc tgacgcacct gctttgcccc ggcaaagtga
     7381 gcaagcagct cttccgcatt gccgcaagct ataaaatcac gtacctgtag aagttctccg
     7441 gcaaataaat cgagcattgc cagcagattg tcccgattag acaagcagat ctcttgccac
     7501 atctgcggac tgcccaaggc aatacgtgtg ctgtcacgga aaccaccacc agccaggctt
     7561 aataactcag tcgtttcgtt gaggctggcc acagtttgta ccagtaccga ggccgcaagg
     7621 tggggcagat gggaaattgc ggccacttgc cggtcatgct gctgtggggt aaaaacaacc
     7681 ggcagaccgc ccagccgacg cactaagtgc tgcatctctg tcaattgcgg tgatggtaac
     7741 ccggttaaaa tatagatagc gttttcaaaa agatctgctt cagccgctaa aacaccttct
     7801 ttttctgaac cggtcatggg atggccgccg ataaaggtaa ctgcagccgg cagcacctgc
     7861 tctgctgcct ccgtcaccac agctttagta ctgcagagat ctgtgacaat agtatcgtcg
     7921 tgaaggtgtg gggctatctc ggcgagcagt tgacagactg ccagcactgg tgcagccagc
     7981 actactacct ctgcttctgc tacagcctct gctacagtag cagcacagcg ggtgatcgcc
     8041 ctaacttctt gcgcttgctt taaggcggca ggatcagcat caaagccgac aacttctttg
     8101 gctaaattgc gttttagcag tgctttgccc agcgatcctc ccatcatccc taccccaata
     8161 attgctactc gacgcagaca aagcatcagg ctggcacctg tgtgatatgt ttgcctaaaa
     8221 ctgctgctac ttgttccagt tctttagtga gggtcgtaaa cttttccaca gttaaagatt
     8281 gggcgccatc acataatgct tggtctgggt ggggatgaac ttctatcatt accccatcag
     8341 caccagcggc tacagctgcc cggctcatac tgggcaccca gcgccaactg cctgtgccgt
     8401 ggctaggatc aacaatcact ggcagatgag ttaaatgctt caccacaggc actgcactta
     8461 aatctaaagt attgcgggtg tatgtttcaa aagtgcgaat accacgctca caaaggatca
     8521 cctggtgatt accactggac ataatgtatt cggcagccat cagccattct tcaatagtgg
     8581 cagataaacc tctttttaag agaattggtt tatccagttt gcccaattcg cgcagcatat
     8641 gaaagttttg catgtttctc gttcccacct gcaaaatatc agcgtattct gctactaacc
     8701 cgacagtatg ttgatccata acttcagtca ctatcggtaa accagtttgc tctcgcaccc
     8761 gggctaaaag tttaagccct tccttctcta acccctggaa ggcataaggt gaagtacgtg
     8821 gtttaaaagc cccgccccgt aaaatttggg caccagcggc tttaacacct tctgctgccg
     8881 ccagcagctg ctcctcactt tccaccgcac acggaccagc catcaacacc acctgctgat
     8941 caccaatggt gcaatcaccc actgtaaaga cagagttttc ttgttgaaat tcacgactca
     9001 ccagcttaaa tggttgtgta atgggcaaca ccttttctac aaaaggcagt gcttccaaac
     9061 tcatttgcct caccagcgtc tcatctccaa tagcaccaat aatcgtgcga aacttcccct
     9121 ctgataaatg tacccctaag cctacctctt ctaaccgtgc tactacctgc tgaatttcct
     9181 ccgtctttac ccctgattgc atgacaatta ccacagtttc tgatcctcct taattctttt
     9241 tagtttatca tactttctcc taccataatc accaaactac aacaagcaat ctcttatcgt
     9301 ttaaaaagct tactcccaat tttcctctgc caagcttata cagcggctag cagcagcaga
     9361 aaaaaatata aaagcaaaca aaaatccgcc ccttgaagaa gggacggaat aattccgcgg
     9421 taccacccaa attggtttat caataaaccc gctcaatttc agcacgccca gtgtgggtta
     9481 tgcctaatcc taggtaacgg taggatctcc gtcgaagcct actacagttc ggttcgccgc
     9541 tccagggggt attttcagtc tgacgcggat accggtttgc agccaccacc ggctctctgt
     9601 aatcctgctg tcctgcctac tatttccctg tcattgcatt ttcatattag tgctaattta
     9661 tcgcttgttg gcagtcttgt caagacaaat tttctgttac tgcggcaaaa gataaatttg
     9721 caattgccgg cgcccaaact taagagcagt ttgatggctg ggcagtaaaa gatcaaggcg
     9781 tttcccttta atcgccccgc ctgtatcagc agcaacagca aagccgtaat catcaatata
     9841 gagacgactc cctaaaggaa tagttttggg gtcaaccgcg acaagatgag gatttgcttc
     9901 actgccatca ccagccacag cccgacggcc gcttgccgta atgccgtcat tatagctgcc
     9961 tgtacaaaca ctgcgccctt tactgtcaat ggggcagccg ctagctgccg tgcccgcaca
    10021 gtaggctgtt gccgatactt tgtagccctg cgtaaaacta atattgcgcc cgttgcggga
    10081 gattacccgg cgttcattac tgccactgct ctgggaggta ctgtcactgc cgcgcgccgc
    10141 aaggaccctg gttgtatttt tgctgccgct gggtttactt ccctggggaa tgactaactc
    10201 tctgcctacc tctagctttg tgggatcggt aatcttattg gccgtcagga ttgcggagac
    10261 ttgagcattg taatgtttag ccaactccca gaccgtttcc cctcgttgca gcgtatgggt
    10321 cagcaaagcc tcactgtcct gggaattatc tttttcttcg ctaaacgtca gtacttgccc
    10381 cacataaatt atattagggt tattaatgtt attgtcatta atcaattgtt caaggctgat
    10441 tccgtattct ttagcaatca tatttagggt ttccccgcgg gcaatagtat gccgtaaagc
    10501 tgtcgccgcg gcccaatctg ccagctgctc ttgcagctgt tgtaaatagg tagggagaaa
    10561 agcactttcc actgcaattg tcggtgcatc cgccaccacc ttgctaggct gtgctgcagc
    10621 gtaaccattt tgcagaagca gcaataaaac aaccgctaaa atggttaaag gaccttttcc
    10681 tttgctcttc atatcttcac ctctttagtt aaactcacag tgtgataaaa ataaaagccc
    10741 acattaaagc aaagatgcct caatgtaggt aattattccc agtatgatgt tttctatact
    10801 agataggaga acaaagcatc aagttcagcg gcaagccgta cattttgctg ccgttgaaat
    10861 taacacaaaa tcagcaaatc atgttgttca cacagaaaaa tacacaaaaa aatcaggcgg
    10921 ttaaacctga tttttagtgc ttttaggatg atcactccgt tggcttgctt aaagaaaaat
    10981 taagctaaaa cattttcatc ttcactgggt ggcgaagata aatgaaagtg ttcatgtagt
    11041 gtgcaaagtg cttctgaaag atcgtcttca ttaatcaaca gggaaatggt gatattggag
    11101 tcgcccgttt gtaaaatcct gattctggcg tccctcaaag ccttaactac actagccatt
    11161 acccctggca gattacgcat acctaaacct accacagcta ctttcgcgca attattgacc
    11221 acacggtaag ttaaaccaag actttctaaa atgctttctg tattcgccag atcagcttca
    11281 acgatcgtaa agctcttacg ctccgggaaa atagaaatca tatcgaggga aatatgcgct
    11341 tttgccaact ggtcaaaaag ctccaattca gcttcggcat cttccgttgc catttccaca
    11401 aagatctgta ccaagcctgg aatatgcgca atgccagtaa tcacacggct tactttgtga
    11461 aaatgcgcct cacgagacgc aatcacttca ttactaatta ctgtgccggc ttcatcttca
    11521 cttaaatgct tcacaatcag cggtacatta tactgcatcg ccacttccac tgcccgggga
    11581 tgaataactt tagccccttc ataggctaac tgacaaactt cattataagt aagatgatca
    11641 ataatccggg cattagctac cagtcgggga tcagcagtca taatgccgtt aacgtcagta
    11701 tgaatttcta ctctttgggc ccctaaacca gctcctaaaa ttaccgcact ggtgtcactg
    11761 ccgccacgac ctaaagtgtt aatttcgcct tctgctgttt cgccctggaa gcccgctacc
    11821 accactactt catcgttacg taaatgagcc attaaccgct ctggttgaaa ctcatctacc
    11881 tgggcgctgc catagttgcc atccgtcact aaaccagcct ggcctccggt caaggctaaa
    11941 gaaggaatac cattggcgca cagcgtggaa gacattacta cggcagaaat gatttcgcca
    12001 caagccataa tcagatccat gtcacgcagg gaaggttctg tctgttcact ggttttgagc
    12061 attaactcct tgagcgtatc agttgcgtag ggctcaccta aacggcccat cgctgaaaca
    12121 acaacgacag gtttaaaacc tgccgcttgt gcgtcacgca cccgcttcac cacttctttg
    12181 cgcagcgcgg gattggcaac agaggtcccg ccgaactttt gtactacgat ttgcaacgtc
    12241 ttccccccta caattggccg cgtgcgatta atgtttctgc aatttgaata gagttggtag
    12301 ctgcaccttt acggatatta tcagccacca cccagatatt gacaccataa ggaactgaat
    12361 agtcaacacg aatacgaccc acaaatactt catcacggcc agccaggtct tgcggcatgg
    12421 gataaagcag ttctgccggg tcatccatca cgacaatacc tggagcgttg cgtaaaactt
    12481 cccgcacttc ttctggggta ataggacgct caaattccac actcactgct tcggagtgcc
    12541 cataaattac tggtacgcgt acagtggtgg aagtaatttt taaatctggt aagcccatga
    12601 ttttttggct ttccttaatt atcttcattt cttctttggt ataaccatct tcaaggaagg
    12661 catcaatttg cggtaccatg ttaaaggcca tttgatgctg cactttggcg ttcgcatggg
    12721 ggaagcgctc cctaggcata gcctcggcgc cattcagtac tgcctgggtt tgtgccgtta
    12781 attcatctac tgcttctttg cctgcaccgg aaacagcttg gtacgaagcg acaacaacac
    12841 gttttaaacc ggctgctttt tgtagtggct gcagtgccac cactaattga atagtggagc
    12901 agttggggtt agcaattatc ccctgatggt tggctaattg ctctggattt acttctggca
    12961 caaccaaagg caggccttca tccatgcgga acgcattact attgtcaatc actactgtac
    13021 cgcgacgtac tgcttccgga gccaaggcca gacttacggc ttctccggca ctgaaaaagg
    13081 cgacatccac gccgtcaaac gcctccgggc aagcctcttc caccgtaaag gtccgaccca
    13141 taacttcaac ctgggtatct acagagcgtg ctgaagctaa caacttcagc tcaccaacag
    13201 gaaaattacg ctcctcaagc acttgtacca aagactgacc aaccatacca gtggcaccaa
    13261 caacagctac attatacttt ttcacttcat tacccccata agtattttaa ttttcctcag
    13321 ccggctgagg ctgctcccca acattagtta caagaaaaca ccttactact ctgctatttt
    13381 atgggctgct cgctgcattc gtgccggcat gagatcatta cggacaagat cctcatacga
    13441 ctcccgcgct acgaccaagt cggcctgccc acgataaaca aaaacaactg gcggcctggt
    13501 aaaacgattg tagttattag ccatggaata atgataagca ccggtagaca gtaccgctaa
    13561 taaatccccc cgtgccagtt taggcagctt aatatcccaa atcagcatat ctccggactc
    13621 acaagcctta ccggcaatcg aaactgtctc ctctgcttcc tcaagggcgc gattggcaac
    13681 gagtgcttca tattttgctt catataatgc tgtccgcaga ttatccatca tccccccatc
    13741 tactgctgca tagcggcgaa tgccgggaat atctttgatc gtacctactg tatacagtgt
    13801 agttcctgct tcgccgacaa tagaacgacc cggttccacc aacagcttag gtaaggataa
    13861 attatgcgct gcggcctttt ctttaacggt agtggtaatt aactctaaaa agtcgcttac
    13921 aggatgcggg gcatcacttt gcagataacg gatacctaca ccaccgccca aatcaagttc
    13981 cttaatcaca taaccagttt cattattaat ttctgccata aaatccatca ttacctgcgc
    14041 tgctaaatga aagggtttta aatcaaaaat ctgtgaacca atatgacaat gcagaccttg
    14101 cagtactaga tgtttcatct gtaaagccat ttttaccgca gctaaagctt gaccatcggc
    14161 aagaccaaga ccaaactttg aatccagctg ccctgtttga atataatggt gtgtatgcgc
    14221 ctcaatccca ggtttgatgc gaaaataaat attcgctttc ttgccggctg cggccgctat
    14281 actctccaac agttctagtt cagaaatact gtctaccaca aacctgccca cgccaacggc
    14341 caaagcctgt ttgatttcag cgggtgtttt attattgccg tggtaaatca ttttggctgc
    14401 cgggaaccct gccgttagcg ccgtaaaaat ctcgccgcct gaaactacat caagagccaa
    14461 accctcttcg tccagcaagc ggcacatcgc cgtggttaaa aaagctttgc ctgcatacgc
    14521 tacttgactg tggggataca ggcgggagaa ggtatcacga taaataccgc aattgtgccg
    14581 aattaattct tcatccatca cataaagcgg cgtgccaaac tgtttagcca gtgccactgt
    14641 atcacacccg ccaatttcta gatgaccctg ttcattaatc agcattgtgc cagatagaaa
    14701 catcgcgtgc ctccttttgt ttgatagaca gaaagaccga catgctgctg tcggtccacc
    14761 aatatccaga catacagcct caaacccttt cgtgagatag ctcttcatcg cctgtcaagg
    14821 gcacaaaacg atgacaaacc ggcagttatt cactgacgat ccagccgcct gtcctaggga
    14881 aatacttgca ggggcttcgg cggtgtcccc ttttctgctt gttcatggaa ttccccttct
    14941 cctaagcagt acttttgttc accgcgcctc tacctcacct tgtggtgagg catgtataag
    15001 tttgcagaaa gtttatcata ggttataaac tctgtcaatg agcagctcac cacaactcct
    15061 gcttttactg gtcttgaagc cttaatttag ctgccactag gggtaaggtt cgtacctgct
    15121 gccagatccg cggccactgc tgtagatcac cgactactgt gcccacctcc acagtaatcg
    15181 cgggaacgcc cagctcctgg atcacaaagt ccatatactt gcctccgtta tcatcatagt
    15241 actggtaacg gtaacctgtc agcgcagcta acgccgctgc caagcgctga tcacggtcaa
    15301 actgcgctcc tttttgctta tgataccaaa agagccattc tccctgttga tgaaaagaaa
    15361 gtgtcagcag cggctgccgt gctaaggcaa attcagataa agcgcgcgct tccggttcag
    15421 acaacggggt aaaaccttta aaatggcttg gcgccggttc ctcctgcaca ttctctaaaa
    15481 cctcccaaga gatggggaac tgacggttaa gatcaacacc gcggatgttg gccttccagg
    15541 ccttaaaact ctcaatctcc tcactagccg ccagcatttg ctccactaaa gccccattgg
    15601 taatcgcttg ggggccagct tgcaccaagt ttagaccatc gggattcacc atcggtataa
    15661 accaaaaaga tacttccttg aggagtgctg cgacatcatg tgaagcaaaa tccgtttggt
    15721 aatgggcatg atgtgcatac tcttcagcca gtttcatcac cagaggtgcc gtcagccatt
    15781 ccgaggcatg aattgccgca tcaagaatga tctcctcttc accctcacct aaacgcaagg
    15841 cataaattgc acgtccctct accgactccc ctatcacctg cagttttagc agttctggat
    15901 aggacgtcac taaacgctgc agatcctctt ccagctgctg gtaggaataa acctgctgcg
    15961 gtgacaccac cgccggttca ttctgcagac gcagctgcgg gaatactgct cttgccaaat
    16021 cctgctgtgc agcctgccag cttacctgct ctgccgcttt aaacaagcgc gtacccggca
    16081 cctcaacaat tcctaattgg gcccacgcct ccaaaggtag tatttccccc tcaataccca
    16141 aaaagtattc ccgcggcgcc gctgtcagct gttcgttagg cgttctggcc aagatctgat
    16201 aaaattgctg ctgtgacagt gcagtcgttg tactgccaag gagcctttct gtttccgcca
    16261 cgctgcgaaa cgcagtaaag tgtcctctca aagccgcata acccagccta accatcgccg
    16321 gagataccgt agtctcaatc ggaactgctg ccggcggttc atgctgcggg ctatcggcag
    16381 tagtcggcag ggccagcata atgagcagga ttaaagaagt gaaaagataa aggagatatt
    16441 ttaccagcaa acgcatgatg agtactccct tgctttgtat aattatatct attacctatt
    16501 tgacagaaaa cggcacttcc ctgctgcggg aaatgccgtt atttcaactt acagtttatt
    16561 cgctggcagc ttccagccct aacttcctag cagcctctaa atactgtggt tctttgagaa
    16621 tgtcgccttt gccgtcgaca gctcgtacca gcaaacgtcc ggccatttta tagcccacag
    16681 cttcaaaaaa gtactgcatc gtccaaatac tacctttaaa caggcgagct cctttagtcg
    16741 cagccacgct gataaagtag ccttttttac cttgcctgaa acgtggatct tttaaaatat
    16801 ggtttcttgc ccataaggcc tgtgagcgat caatagcttt cttggcccaa gcagaaacat
    16861 tgtaaaaata atttggtgag gcaaaaatca tgacatcagc tgcccctatt tttttatata
    16921 catcctgcat gtcatcctta aagatgcact ccccagtttt ttcacactct ccacaggaaa
    16981 tacaaggaga aaaatccagt gtacttaaga cgactttctc tacctcggcg ccagcttgct
    17041 tagcgctagc caatgcttca tccaacaata tctccgtatt acccttcctc cgggggctcc
    17101 ccgcgatccc taatactagc attgctcatc ctccggtcct gtatatggtt cgggcagaat
    17161 ctcaattact gaaacctcga gctgcctgct ggcaactttg cgatcataac cacgcactaa
    17221 aagataggaa agcaggagga aagcagcacc tagcaaaata tcaaaacgga agccgcctgc
    17281 tgtaccatct aactgctgtc cgataaaaag ccctaataac aaagccagca gcggcactat
    17341 gtacaccata aaagacgccg taagaatatg cgaggtatca ctataaatct tcactgtttg
    17401 tcctagattg gcattaactt tatttagtgc ctccaaatgt gtttcaccac gtccggaaac
    17461 tgcgacaccg cagccgccgc attttgtgca tacattatgg cgttgtacag caacaatagc
    17521 tttatcatcc tttacttcaa caacaagtcc aacttgttcc acggacttca cctcctgtgt
    17581 tcatttttcc acagttaaga aactataaac ctaatatata aacattatat atttaataaa
    17641 accatttgcc aacccatcaa taagctagcc attaatattc ataagctgaa accctctggc
    17701 agacaagaga agtacacggc tctggataaa gataaataag gcaagaaccc ttgaacgaag
    17761 ggttcttgcc ttattagttt tgaaggtcac gaattactct tttttatact cggttggttt
    17821 aaccgaagga atgcagaccg gttagtaacc aggccacacc tacccaggtg aagaccatgc
    17881 aaacccagcc gatcatcact agcgctattg tacgcaaacc tgtccagccg cgggtaaaac
    17941 gagcgtgtag gtagacagca tagacaaacc aggtaattaa agcccacatc tccttgggat
    18001 cccagcccca atagcggccc caggaaatat ccgcccaaat ggcaccggtg ataatcatca
    18061 cgctttgaaa gagaaagcca aaagcagcgc cacgatacat gtactcttcc agcgttttac
    18121 tctggggcag gttacgggtg atgagatgat ctgctttaag attaacttta agcagccaca
    18181 tgatcgctgc ggcaaaagct agtgtgaagc cggcataacc aaaaatcgaa gcactaacgt
    18241 gcaccgttaa ccagttattt tgcaggactg gtgccagctg ggtatgcgca cggctgatac
    18301 gaatagcggg aaatagccgc ggcactaccg ttaggtaacc tagataaagc atcgccagcg
    18361 gcgtcacaaa ggcgccgaca gcataatatt gctctttcat ccgccattct gtccataaat
    18421 aaactaaaac agtcgcccac acaaacatat tgccaaattc gaaactcccc acaaatggta
    18481 cgcgctgcac attaagcgcc cgcgtaacaa taaaaccggt gtgaagtaag aatgacactc
    18541 gtagaatata ggaggcaaaa cgtccaagct gctttttacc cagcaaatgc gccacatagc
    18601 acaaggtagc aaacaaatat gtcaccagcg ccagattaaa aaatgtgttc tcccaaaata
    18661 atcccatagg agacacttga atatagctag acacgtgctt ctcccccttt gcttatttcc
    18721 tgatttatct gctctaattc ttgctctagg agcagacggt tacggctgcg accaccaata
    18781 atcaaggagt tactttcctg agcgaagacc gcccaaaagc gacggggaaa agtaaagaaa
    18841 cttaaatata gccccaggat aagcagcacg gcaccggtaa aaagaatagg ccgtcctaaa
    18901 tttcgtgtaa ttgtaaaaac tgctgcattc tcaaaaccgg tcatcagcag ctctatctca
    18961 ccagtgctgg ttttaatcac ttctggctca tttaatatga gaaaaacatt acgtaccgcc
    19021 ttgttattct cgatcagtaa ccctgccaat actgggtttt caggcaaata gttcttggag
    19081 tacggtacac cgcccccgtg catggaaaaa ttggggaaaa actgcatcac atgcaggata
    19141 accttacctt cttcatccag ctgcagttga tcaatatccc ataactttaa ttgctgttct
    19201 ttaccagtag ccaggtctgt ggctacaaca tctgaaacat agcgaaaagt agtatggtaa
    19261 atcgtattgc cgttaaaacg cagcggttga ttaatagcaa ctgtgccttc agataacggt
    19321 tggtcatccc tagtgataac caactcacta aagacacgcg gccgcactgt cgggtcagct
    19381 tcctgctcca tcttgacatc cttgatatct acacggaagt cgccttgttc cacatggaaa
    19441 tgatctccgg ccagaccgcc gttacgagct tcaaagcccg tcagtgaacc atacatggcg
    19501 cccagtaaga ttacaagtaa actaagatga gtcaccaaag aaccaaagta gccgatttta
    19561 ccgcgttcgg caaacaaaac ccggccctgc tggccagcag taacttctac ctgcaaaccg
    19621 ttctttttaa aggcagcttc tgcctgcgcc aacgctgctt ctgctgtgac cttctccatt
    19681 tttagctcat aatggtgagg catttgacgt agtgctgccg gtgaagtcag tttatggggg
    19741 tgaaaagcaa gctttgtcac tggtttaatc cgtcgggcag tacataaaag taagtttagg
    19801 gccgtaaaag ccatcatgcc aagataccac caagaatgat agatgctggt tatagctgtt
    19861 tcattctctt tcagtgtagc aaagactgaa attacagcca gtaacaaaag taaaatgatg
    19921 cctagctgca tcgaactcaa gaaattcagc agctgctgca gtgggctttt taccgctgtt
    19981 ttaggtgttt cttttgtcaa gattgtcccc tcctcactct gccatcatca gcggttgcta
    20041 gctgattatt tttcaatctc atctggatgt tcttccgctt catatttgga tgggcacttg
    20101 accaagatgt tatctgccag aaagtctgca ccgctatatt tgcctttcgc tactacgctt
    20161 tccgctgcaa atagattgtc gggtgcctga ccgctggcca caacacgtgc cacttcacca
    20221 ttttgatctt ccatctggaa aacatattcg ccgtcagcaa gctcctccag ggtacctgcc
    20281 acagcgaggc ctttcacctg cacagcacgt tccgtagctt tagcttctgc aaaggtaaca
    20341 taagtgctga gggcattgcg cgcagtcata aagatgccca caccaaagac taccagtaag
    20401 attaggagga ctattttatc atatctcttc acgccgctct gcctccttca cctttaaatc
    20461 aacccgcaat aaatagaaaa atagaccaag ccaagtaatt aaagtcactg ctaacacata
    20521 agataaacct tcagccatcc tactcccccc tcttcatgtc tatggcataa agtaaatttt
    20581 ccagtcgcat ctgcacctta tacatccagg cataaagacc tgtaaaacca agcagcgagg
    20641 aaagaaaaac taccagcatt ttacgatcca tatcaaagga gcggttttca agattaatta
    20701 aatctgggtg cagggagaca taaacacgcg gcacaataaa aacaagaaac ggtacagtta
    20761 aaaaagccaa aatcgcataa acagcagcta acctagctct gcgatcagca tcagggatcg
    20821 cactgcgcag cacaaaataa gcagcataaa tcattaataa gactgtaata gaagtctggc
    20881 gcgggtccca attccaatac tgtccccagg caccgcggct ccagatcgca ccggtaacgg
    20941 tggcgagtat acaaaaaatt gtacctaatt ctgcattaac ctgtgcttga cgatcccagc
    21001 gcagatcccc ctgacgcaaa tagccaagag aatttaccat cgccattaag tacgccaaga
    21061 cggctatcca cgctgacggt acatggtaga tcaaaatacg gcctgtctct cccagacctt
    21121 gtgttgccgg catgtataaa aaagaggcga taattacacc caccatccag attcctaaaa
    21181 taatttgcca gaacatcttt cgccctccat ccgctgttat cattatcaca agctatcacg
    21241 tatctttatt cgttccaaac atactcaaaa agcagcagcg aagctgccag cgccacccca
    21301 ttataaaaga ataacattaa taagtcagcc gtaacatctg ccgccaatcc tccacctaga
    21361 gctgcccggg tggcgctaat cgccgcaaat agcaacggta ataaaactgg aaaagctagg
    21421 acagtcatga gcgagctttt agctcccgct ttggccacaa tggctgccag aatagtgctg
    21481 gcgccggcca aaccaagtga gcccaaaact actgcagcta caaaggccag tggcagggca
    21541 atacttaagt tcagcatcat aataaaaagc ggtactacca acacggtcag gcttaataag
    21601 atagtgacat taaagagata cttgccaagc aaaaccggct cgggctgcgc tgccattttt
    21661 agtgcggttg ctgtacccgt ttcttcttcg gcgacaaagg tgcgggctaa tccggccatt
    21721 gccgaaaaaa agaggataat ccacagtaaa gctgccgtaa tatcagtatc tgtaacaaaa
    21781 cccccaatgg aaaagctaac cgtgactaga gtggtgatgg caaacatcac taaagcacta
    21841 taagtataac gagtgcgaaa ttcgatgcgg agatctttgc gccaaatagc tagtccttgt
    21901 ttaagccagc gtaaaggttt gatcaccgta ggccacctcc cccggttcat tggtcgccat
    21961 gatcacaatc ccacgctctt tttgctggcg gataatggta tccacaagtg ctacccctgc
    22021 ttcatcaaga ttagaggttg gctcgtctaa gaccaagata tggggttgat gcagcagagc
    22081 ataagcatat tttatccgct gtttcatgcc ggaggagtat gtctttaaga agtcatgccc
    22141 acgccccttt aaccccacaa atgctaataa ctcccgcgcc tgctgccggg aaaagggcaa
    22201 ctcacgcacc ccggcaaaaa actccaggtt ctcgactgct gacaattcac cgtacaattc
    22261 tagctccgga gttaccagac caagctgcgc acgtcgctga ccggccgtca agatttgtcc
    22321 gttaatcata atatccactt gccctgaagt cgcaggggtt aacccacaaa ggatgcgcag
    22381 gaaagttgtt ttacctgcgc cattggggcc caggaccaca agcacagaat tatcagggat
    22441 agttaaggaa acatcggtaa atagtttacg acgttgataa gctttgctta agtttttaac
    22501 tacaacttgg ttcaacatcc tcgacactcc tgctggtgaa acttgggcag atttacctac
    22561 cacgagtata ccacccagtg tggtgagaca agtgaacaaa gtgtgtccaa aaaatgaact
    22621 ttctttcatc ctggctgcct aaacagccag aaagaaagcc tgccgccggc agcgcaagcc
    22681 ttaaatcagc agagggtact cggcgtaccc ctctgcttac tagttggcaa attgctgcag
    22741 ccataaggcc aaaatcacca gcacccatat tttccggcta tggtctgcct ggccctcacg
    22801 atgttctgcc agcaaagtac gtatataggc acggttaaaa tactgggcta agagcggcgt
    22861 attaagcagc tccgctacct cttttgctaa cggtccacgc agccacacac gtgtgggaac
    22921 aggaaaacca cgtttgggcc gtttaatcac ttccggcggc agaagttcag caaaggcttg
    22981 ccgcagcaca tattttgtca tgccgccttt aattttatag gctgagggaa tggccgccgc
    23041 atattcaaag acctgctgat ctaaaaatgg gacccgcagc tccaaactat tggccatcgt
    23101 catcttgtct gctttaacca ggatatcacc aaccgcccaa gtatgcagat ctacatactg
    23161 catttttgtc acatcatcaa aagctgccat ttgccggtac agcggagccg tgattactgt
    23221 agccggggcg aatctctttt gcttcaatat tttttgtttc tcggcctcat taaagatgca
    23281 ggcattgcca aaaaacctat tttctaaggg ggttgccgca cgacgtaaaa agttcttccc
    23341 gggcaaaaaa ccaggccaaa gttttgctgc tgctgccagc agcggctgtg gtaaatgttg
    23401 ggctaatttt aaatcaccag gctctttata gataccgtac ccgccaaaga gttcatctgc
    23461 gccttctcct gacaaggcaa cttttatatc cttgctggct agccgtgcca cataatataa
    23521 agcaatccct gccggatcag ccactgggtc atcaaagtgc cagaccagct gcggtaaatt
    23581 acgccaatac tcctcagcac cgatgacata ttcatgatgg ttagtttcca agtaacgagc
    23641 cgtcgctgcc gcgtctgata gttcgctgta accttcttcc tcataaccta cactataagt
    23701 agacacaggt cccaattcac gcagcaacgc cacaatcaca gtggagtcaa tgcctccgga
    23761 taaaaatgct ccccacggca cctcagcttg agtatgtaga cgtacagatt cgcgcattat
    23821 tgcttgcgtt ccctcgagaa gttcttctaa cggtctgtcc tgcggcgcaa aggcaatctg
    23881 ccaatagcgt gtcaggctta atttgccttc acgccactta aagctatggg caggtggaat
    23941 tttataaaca ccggcaaaca ttgtctctgg ttcaggtaca tattgaaaag ttaagtaatg
    24001 aggaattgct gcagcattgg gctgcacccg aaaagcaggc agtttggtaa aagccttggc
    24061 ctctgaagct aaagcaaaat agtctgctgt ttccgtataa tacagtggtt taatgccaaa
    24121 atgatcccgt gcgccaaaga gctgtttgtt gcgttgatca tacagacaaa aagcaaacat
    24181 tccccgtagt ttagttacgc aggcttcacc ttcttcttca tataaatgca agacgacctc
    24241 gtagtcactg tcactgcgaa aaacatggcc gcgtaagagt aatccttcac gtaactctcg
    24301 gtagttatag attttaccat caaagatgag tgctagggtc ccgtcctcat tcagcatggg
    24361 ctggcgtccg cgtgcagaca aatccataat ttttaaacga cgagagccta aagctaccga
    24421 gccaagataa agggaatcag cttcatctgg cccgcggtgt cgcatcgctg ccgtcactgc
    24481 atcaagatca cgctctagac gactactagt tttattccag agtccaataa aaccactcat
    24541 tgactgtaaa ctcccttcag taaacaaaag ttcaaaaaaa ttgttctcct tataatatgt
    24601 agggcaaagc ttaaatggaa ttgctatttt tatgcataac aaatttacct tcaccagata
    24661 ctaaaaccag taatggaaag gaggtgtagt cgatgtgtca ccgtgaaaca aactatgaat
    24721 ttgcgataat gccggacaac gagattagta aaatccgtga tgtagaaaaa acgctatcgc
    24781 aaaatggtga agttatattg ttggcctaca aacgttcaga ccaaaatgtg atggatgatt
    24841 gagaataaaa taaggctgcc ggtcggcagc ctcaaaaaaa gctgacagag cttgtcctct
    24901 gccagctttt ttgtctggat aattttattt tgcagtttta gggttactat aattttaatt
    24961 cataatcccc aggcttaata cggccgcttt tgcgcagcca ctcgctaaag aataaggtca
    25021 aaatagcggg taaaagaaaa tgcagcaaaa taatcttaaa caaaactggc atggtaaaac
    25081 ccattactgt tgctgtacca aactgtccca caaaaccact ggtccccatc ccagcaccag
    25141 ctgggttatt gtacattttt aaaatggttg tcgctagcgg cccgatgaga gctgaagcca
    25201 aagtgggagg cacaacaatc cagggattac gcacaatgtt aggcacctgc agcatggaag
    25261 tgccaacccc ttgggcaatc agtccacccc aaccattttc ccggtaactg gccacggcaa
    25321 aaccaatcat ctgcgctgaa caacctacgg tggcggcacc agcggccaac cctgagagat
    25381 tcatcataat ggctaaggcg gcactggaaa tgggtgcagt tagcgccaag cccattaaga
    25441 cagaaacaat gatacccata ggaataggct gtaattctgt cgcgcgcata attaactgtc
    25501 caaaactctg cataaagttg tttacccagg gtcctaccac cgctgctgct agcaagccgg
    25561 ctaaaatcgt aaccgcaggc gcaacaatga tatccacttt tgtttcttta gagactgcct
    25621 tgccaaattc cgcaccaaaa acagtcgcca caaaactgcc ggctggaccg cccaatgatg
    25681 ctccggccgc accggtaatc acactagcaa agagcactaa aggcggtgct ttgagaccat
    25741 aagctacagc aatgccaatt gctggcccca tcatactttg ggctgtttta ccactgttga
    25801 ttaaaaaagt aaaaatagcg tttgcaccta ttaatgcggt caactgttcc ccaagcgttg
    25861 ctaagatcag gccaacaatt agtgaggaaa atagaccaag cgccatcgcc cccagagcat
    25921 caataaaata acgctccagt gaaaaaacaa tattctttct tgccagaaaa ccctgttttt
    25981 ctgtcatcct gaaaccccca acttttataa gttttacgct aagaccttga tctgacctgc
    26041 tgcacctgcc gtgacgcgac caattactgc cgccggcaca tcacgtgcgg ctaaagtttg
    26101 tattagagct gcttccttgg cggcaggcac gctgatcaac agtccaccag aagtctgggg
    26161 atcacataag acatctagtt cacgatcccc aactgccggc ccaaccactt gcaggccatc
    26221 tgccacatac tcccgattgg cataggcacc accaggaacc agtcccattt ccgcagcacg
    26281 taaagccccg ggcagaagcg ggatcctgcc aaatgataat tctatagcta caccactggc
    26341 cagcgccagt tccctggcat ggcctaaaag gccaaaacca gtaatatcag tagccgcaga
    26401 gacgccaatt tcagtcatca ccgctgctgc ctctttgttt aatgtgatca ttgtctgtac
    26461 cgccgcctcc aactcaccgc tgccagccat ctctgctttt accgctgtca tgatcaaacc
    26521 agaaccaagt ggtttggtta ggactaaggc atcaccaatt tgcgccgcac tattggtaat
    26581 cagctgctga gggtgaacaa aaccagtaac tgagaggcca tatttaggtt cgttgtcctc
    26641 cacactatgt ccgccaacaa gcaaagcacc ggcctccagc accttatcag caccgccctt
    26701 gaggatctca gccaaaggcg ccgtaccaag agttttggca ggatagcaga caatattcat
    26761 gaccaaccgc ggctgtgctc ccatggcata aacatcggac aaggcattag tagcagcaat
    26821 ttgaccaaag acatagggat catctacgac aggtgtaaaa aaatccaggg tttgtactag
    26881 cgctatatct tcacgtaagc gataaacacc agcatcggca aaagagtcgc aacctatgag
    26941 cagattttca tcattcattt tcggcaattg acacagcacc tgtgccagtg ccccgggggg
    27001 catcttggct gctcagccac cacttttggt taatgttgtt agccgaggct gctcattagt
    27061 catcttttcc atcctcccca caatccgtaa tatccaaacg ccacgcgaca cgttcatcac
    27121 gcagccggcg tgtccacttc agatgatcga atttttccag cagcggaatg acatacttcc
    27181 gcgtggtgtt taatttttcc cgaaactcgc cagtagttaa agtttggtga gcacgaaaat
    27241 gttggcacaa aacttctttg gcttgctgaa agaattcttg atgcagtgct aactgttcat
    27301 ctaaacgcac cagcagaccc tgctgtgcta tataactaag gtatgtctct atttgcgcgg
    27361 tggttaaaac tagcttggca gccgcctctt tactcgtagg cggcataaac ccaccacttt
    27421 ttattagttc cgttaaagct gtgatctgtt ttgtttcctt ttcacttggc tgtgggtgat
    27481 gtgccgccaa ggcgactaaa tcttcataaa gacgcaagcg ttctgctgtt accaaagttg
    27541 tcaacagcca gtcataaagt tttggcgtaa cctgctgcgg cagcgtcttt tttaatgctg
    27601 cccgcgacat acccaccgct aaatgatagg cttggtgata ctctgttaac acctgctcca
    27661 gccgctctga ccactcctgg gccgctaaag catcaacata cagttctcct acctgcttta
    27721 ttttaccatc tgtcagcaat ttgcttaatt ctgtttgcag cacctcaggg gctagaccag
    27781 actccttcag cagcagacta tgagcaactg ccccctcacg gcggatgcgt tgtaaaatcg
    27841 gcgcagcagg atctcctttt tccagttcct ttagtttatg taaaatatca tttttgaaac
    27901 gcttatgttt gctagggcga gcatcaagca ccagcccgcc gccaatggtg gtcatcggtg
    27961 aaaaactacg gataataaaa cggtcgtggc gctgcgcgac taattttcgt tccagacgca
    28021 gctgcacggg cgcttcctca ccctgtttta attcatctct atccagtagt gctacccgcc
    28081 ccacagcgcg tcccgtaccc aaataaagat gtacgcgtgt cagatttttc atagctcgtg
    28141 gtgcagaggc taaaagcttt aatttagcat ctatcaagta ggtcgcttcc agggaatcaa
    28201 gtgccgcgat gacactgccg cggggcagtg cctccttttc taaaccagaa agattaacgg
    28261 ctgtccgttg tccggccgcg gccgactcta cctgtgcccc atgcacttga atctgacgca
    28321 cccgtgccgt acgcccaagg ggcagaactt gtacagttga acctagatgg atagtacctg
    28381 ccagtaaggt acctgtggca atagtaccaa aaccaggcac tgtaaagacg cgatccacgg
    28441 gtaaacgcag cggagctttt tcatctctgt tttcaatatc tgccgtcagc tcatcaatgg
    28501 cctgcaataa ttccggcagt ccctgccgtg tgtgtgcaga tactcgatag taaggtgctt
    28561 gcgctaaaaa agtgccagct agagcttcac gtacttcttc ttccactaaa tcaagccagt
    28621 ctacatcttc tactaaatct atttttgcca gtaccaccag cccacggggg atatgcaaaa
    28681 gatccatgat ctctacgtgc tcacgggttt gcggcataac tccttctgtc acatccacta
    28741 ccagcaggac taaatcaata ccgccaatac cggccagcat attatgaata aaccgttcat
    28801 gtcccggcac atcaatgacc ccagccaaac gctcgctagg taatttaaag ggggcaaaac
    28861 caagttcaat ggaaataccc cgctctttct cttcatgtag tcgatccgta tcttcaccag
    28921 ttagtgcttt aattaaagca gttttgccgt gatcaacatg ccccgccgtg ccaatgatta
    28981 cgtgagacac tgaaccacct cttcttcttc gctttccgca atcgtgcgcg ggtccaacag
    29041 cagccagtca tcctgtacgc gacccaccaa agctggcgta cccagccgta attgtgctac
    29101 ccactggtgg agtggcactt gccgatggcg caccgctatc acgtaagtag gcagataggt
    29161 tgtcggcata gcaccgccgc caacatagga aacatcctcc cgcacttcga cgatcaagcg
    29221 tgcttgggct tgtgcggtaa tcgccgccgc tagcttggca gcccgctgcc gcagggccgc
    29281 agcagtaata gatagtttcg ccaacacggg cacttcctgc aaagggtttc cggttacata
    29341 aatttgcagg gtagcctcca gagccgctaa agtaaattta tcaggacgca aagcccgcaa
    29401 tagctggttg cgcttaattt gttcaatata tttacgcttg ccaacaatga tacccgcctg
    29461 gggaccgcct aaaagcttat ccccgctgag tgttaaaaca tcaacgccgg ccgcgatacg
    29521 ctccgtgatc aatggttctt tagccaaacc aaaagcagaa agatctaata acatcccgct
    29581 gcctaaatcc tccatcaccg gcaaatttac ttcttgcgcc agctccacca gggctgccac
    29641 tcccacagcg gcagtaaagc ccacgacttg atagttgctt gtatgtacct ttaataaaag
    29701 agctgtttct tcaccaacag cattccgata atcgcgcaga tgtgttttat ttgtcgtgcc
    29761 cacttccact aactcagccc cgctggcggc catcacttcc ggtatccgga aactaccgcc
    29821 aatctccacc agttccccac gggaaacgat gacttgtttg ccggcagcca aggtgtttaa
    29881 ggctacgaac acagcagcag cattattatt aaccacacag gcagcttcag caccagttag
    29941 gcgctgcagc aatttttcca catgcacatg acgtgagcct cgcttacctg attgcagttc
    30001 aagttccaaa ttgcaatagt gggcggcctc agtaatagct gtagtagccg cctgcgctaa
    30061 aggagcacga cctaaattag tatgtaatac cgttccagta gcattaatca cccggcgcaa
    30121 agaaggttgc tttaacgact ccagtctagc ctgtaccact gaagccacag cctgtggggt
    30181 taagtttaaa gttactaact tctctgctgc gggagcggca agaatttgct gacgcagctg
    30241 cgccagcact tcctgtacga cctggaccag taaagtatgt gcataatgct catcagtaaa
    30301 aacagactga taagacatca gcactgcatc aactgccggc aaagaacgca aatattcatt
    30361 tcgccagttt tccaccatat ttttcaccta ctgacgtatt tttcattaag cttcatccca
    30421 attattatat agcttaaata aaagtaacgc aacaaaatct gccggcgtga cagggaaccg
    30481 tcccctctta cgggcgtggc aaatatacgg aaaaggagct tccttctccg ggctcacttt
    30541 ccacggttac ccgtcccccc atggcttcga ctaattcctt gacaatggct aggccgatcc
    30601 ctgaaccgcc ggtagtgcgg gcccgggaag gatcaacgcg ataaaagcgt tcaaagagat
    30661 agggcagatg ttcagcagca atgccgatac cggtatcctt gacaactaac cctacttcag
    30721 cagcctgctg aaaaagggtg accttgatca ccccgcctag cggtgtatac ttataagcat
    30781 tgtttagtag attgttgagc acacgcccta acagggtttc atctgcccgc acatattctg
    30841 tttgtgctgg cagctctaac tgcagatcga gctcttttct ttgcagcagc ggcttcagac
    30901 gttccacttc cgtctgtagc agttcattaa aatcgagcac ttttagcgtc acgttacgtg
    30961 tatgaaagtc cgcatttgcc agctcttgta aatcatttac taaactccct aaacgagttg
    31021 actcctctag cacagcggca atgttctctt catcaggagg taatacatca tcgcgaaatg
    31081 cttccaaata gctttgaatg gttgttaaag gagtacgtag ttcatgggag atatcaccag
    31141 taagtttgcg gcgcagagca ttttgatcag caattttagc cgccatacga ttcatcgttg
    31201 tccctaacac cgccaactca tcctcaccat ctacctccac acgtgcttca atttgcccgc
    31261 ttccccactt atcagctgtt tccgtaagtg actgcaggcg agctgttaga cgacgtgcta
    31321 ataaaccagc aagaatgatc gccgccacag cggctaacag gccagtaatt aggccacttt
    31381 gctgcactgt ctgctggaaa actagcgcct ctgttgtcca taacccttcc tgccctagat
    31441 gggtaatttc tacagtacct actgttgttt gaccggctac aagcgggtaa ctgtaagtat
    31501 ttccttttgc ttgctgggca ttctgccagt gcctgccgtg tttcctctgc atcatgcccg
    31561 ttaaagaatc aacgatcaag tctccatggg catcaaagac acgaatttgc gcacccgtgg
    31621 tattaccaac atagagtgta gccagacgaa tggactgcca tgaggtactc tcactatata
    31681 gatcagccag tgttcccaga atcaaacgat tctgttgttc ctgtacatta tctaaataac
    31741 gttcgaaatt ccatcctagt gctaattgcg taacaagcgc aaagactaag attaacccga
    31801 cagctaaaat gatcatggct attgtaaatc tgcgggaaag actatttttc atctttagtt
    31861 accttcaaac ttatagccaa cgccataaac tgtacgtata aaacgaggct cattggcatt
    31921 gggctccagc ttatgccgta aatttttgat gtggctatca attgagcgat cataaccggt
    31981 agcatagacc ccttgagcta tctccaacag ttccgcccgt gaaaataccc ggccggggct
    32041 tttggccatt gccactaaca gtttatattc tgtggccgtt aactctacta tttggtcacg
    32101 aaagcttact tgatgacaca gggtattaat tactaactcg ccatcagcat attccagaac
    32161 atcggccaac accccagggg ctgaacgcac acgtcgcaat actgccagca cacgagctac
    32221 cagttcaccg gggctgaaag gttttacaac ataatcatcg gcccccaggc ccaagcctaa
    32281 aatgcgctcc tcttcgcccc ctttggcagt caacatgatg atagggatct tgttgccagt
    32341 ttgtcgcagc cgtttggtta cttcctcgcc agaaaaaacg ggcagcatca aatccaacac
    32401 cattaaagca aaatcaccac tttgggccaa ttttaaggcc tctgcaccat cataggcttc
    32461 agttacagca aatccttctt tctgcaggta agctgtaatc acccgtgtga ttttctgttc
    32521 atcatccacg accaaaatcc gctccatccc tgctcacctc cagctgatcg ggtttatcta
    32581 attgagctaa ttttatcaaa ccagcaaaaa caatgcgagc tttttgataa aacgttcctc
    32641 aagaaaaaga aagccggcac aagtccggct taattgctta taaggctgat tgttcaggtg
    32701 tgaccgcact accttgttga ttgtaggggc agttgccgca gttaccattt ctccgaccgc
    32761 cccggccgcc gccacgacct tgagggccaa aagaatagtt gccttcctgc atgctctttt
    32821 catgatcctg ccactgttca gtcatgcgct cttgcatcac tttagcatct tcttttgtca
    32881 atacgcctga ttctgcttgt ttttctacga tttgcaagcg aagctggtgc tcctgctcat
    32941 aaagtttcgc tagttcatcc ttttgtttgt cagttaaagc catcgccgca ggtacagcag
    33001 ctgctaagac caacattacc actaaggcta ataataatcc ctttttcact attacacctc
    33061 cttatctact tctttgtcat ccatagtata taccaacttt gtcaagaact aatggaggaa
    33121 tgatggagat tttgtggaga ggataaatat ctatgaaaac aggcaattta gaagtgagag
    33181 gccgtttagt ttaggagggt caaatatgaa acaaaagaag ttaaaaaagg cactgctcat
    33241 aggtgcaggg gcggccactg gtctgactgc agccaaactt atggtcagag gagcgattaa
    33301 caaaacacat gatcatgccc tgaaaattct catggaagat aagtatgatg aaaatatgtg
    33361 ggaactgatt tcagccgcaa cccgcattgg tttgcaaaat gtgctagaga caaatctgcg
    33421 tgccactgaa gggaaagcta tcgcccgtcc catgggttca cccaaaaagt ttcccagttt
    33481 agatgatcta gtcttcactt tcgcgcaaat gtatataatg cccacgccac tagaagcaaa
    33541 tatcgatacc acggtagtca ttggcaaaaa agcgaaaaaa ccattcacgg tcgattttcc
    33601 cataatgatc gcacccatgg cctatggtga agccctaagt aaaaaagcga aaattgcgct
    33661 ggctaaaggg gccgcactgg ctggtacatc taccaatacc ggcgaaggac cctttctccc
    33721 cgaagagcgt aaagctgcta aatatttaat ttttcagttt aaccgtggtg attggaacaa
    33781 agacccagag atcatccgcc aatgtgatgc tatagaaatt cagattggcc aaggggcctt
    33841 agggggagtg ggtcatgtga tgcctgcgga aatgattgat aaagagttgc gtgaagcttt
    33901 tagttttccc aaaggcaaag atgctgtggc tcattctcgg caaccaggcg tagaaagtcc
    33961 cgaggatttt actaggctgg taagcagact aaaagagcaa ggtgaaggta ttcccattgg
    34021 ggtcaaaatg gctgccggca aacatctaga agctgattta cgtattctct gtgaggccgg
    34081 catcgattat attgcgatgg aaggagctga agccgccacc aagtcttcag cgccaatttt
    34141 acaagatgac tttggagtac cgatggtttt tgccatcagt cgtgctgcta actggctaaa
    34201 agaaaatggg tacagcgaca aagtgacact gattgccagc ggtaaaatgc gcacccccgg
    34261 ggatgtgctt aaggcatgtg ctctgggagc tgatgctgtg tatatgggag caacagcact
    34321 ttttgctgtt gctcatacac aagtactaaa accactacct tttgagccgc ccacacaaat
    34381 agtttggcat aatggcaaat acgctaaaca atttagcatt aaaaaaggca caaaatattt
    34441 atttcaatt
//
LOCUS       DASRMS010000012         1430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_270499, whole genome shotgun sequence.
ACCESSION   DASRMS010000012 DASRMS010000000
VERSION     DASRMS010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_270499"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="VFC74_00825"
     CDS             complement(<1..210)
                     /locus_tag="VFC74_00825"
                     /inference="COORDINATES: protein motif:HMM:NF016961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZK23913.1"
                     /translation="MENNRIIKAVLAGGATTLHFLFGGWSLPLQILVAVVTIDYLTGI
                     GAAFVGKRLDSSIGSKGIVKKVGFFV"
     gene            complement(233..457)
                     /locus_tag="VFC74_00830"
     CDS             complement(233..457)
                     /locus_tag="VFC74_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23914.1"
                     /translation="MPKDKQKHLLAGLALSTLAGLLFCPLVGLVTAAAVGALKEIIWD
                     WLLKKGTPEFLDFVATVAGGVIGCVLIGWL"
     gene            complement(507..>1430)
                     /locus_tag="VFC74_00835"
     CDS             complement(507..>1430)
                     /locus_tag="VFC74_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23915.1"
                     /translation="VTTTKINTGAITETKISDNAISTPKIKAGAIEADKIAANAVTAN
                     KIAANAVTANKISAGAVETEKLAANAITTGKIAAGAIETNHISAGAVKADQIDANAIT
                     ASKIMAGAVTAAKIATRTITALEIKSGAITANEIAAATITAREIAGQTITAAEIAANA
                     ITAGKIQAGVVSYDKLRVAFAVIGSAYLGQPGGGSMNGVIVQAQHQDAWNEILFPGAS
                     FPLNERLWIQLYHQALTTLVGGYMWQINTNNDGWRAIDQSSQTINITGVNVAIDPPSV
                     GQGLLSTTLQNTRPGRSVADIKIRFKRHAIE"
BASE COUNT          307 a          399 c          315 g          409 t
ORIGIN      
        1 gacaaaaaag cccacctttt taacgatccc cttgctccca atactgctat caaggcgttt
       61 cccgacaaac gctgccccta tccctgttaa gtaatcaatt gtgacaaccg cgaccaatat
      121 ctgcaatggc aacgaccacc ctccgaataa aaaatggagc gtagtggctc cgcctgctag
      181 tactgccttg ataatgcgat tattttccat ccctgccctc cttaactctg tgttacaacc
      241 acccaatgag tacacacccg atcaccccac ccgccaccgt ggctacaaaa tccaaaaact
      301 ccggcgtgcc ctttttgagc agccagtccc aaatgatctc tttgagtgct cccaccgccg
      361 cggctgtgac taacccgacg agcgggcaaa acaaaagccc cgccagtgtc gacagggcca
      421 gtccggccag taggtgcttt tgtttgtctt tgggcattag agtagtactc cttcttgcga
      481 ttgttgggtg gttctatttt taaccactat tctatcgcgt ggcgcttaaa ccttatttta
      541 atgtcagcaa cactgcgccc agggcgagtg ttctgcaatg tcgtcgataa caagccttgt
      601 cccacactgg gtgggtctat cgcaacgttt actccagtaa tgttgatagt ttgcgaactc
      661 tgatcaatcg ctctccaccc gtcgttattt gtgttaattt gccacatata acccccaacg
      721 agcgtcgtta aagcttgatg gtacaactgt atccataaac gttcgttcaa tgggaaagag
      781 gcgccgggga acaagatttc attccacgca tcttgatgtt gcgcctgaac tattaccccg
      841 ttcatggaac ctcccccggg ttgaccaagg tacgcgctgc cgataacagc aaatgctacc
      901 cttaacttat catagctaac cacgccagcc tgaattttcc cagccgtgat tgcattggca
      961 gctatctctg ccgcggttat ggtttgccca gctatctctc tagctgtaat agtcgccgcg
     1021 gcgatttcat tggcggttat cgcgcccgat tttatttcga gggccgtgat ggtcctagtt
     1081 gctattttgg cggctgtgac tgcacctgcc ataattttgc ttgccgttat agcattagcg
     1141 tcgatctggt cagctttaac ggcaccagca ctaatgtgat tagtctcaat ggcgccagcg
     1201 gcaatcttcc ccgtcgtgat agcgtttgcg gcaagttttt ccgtttctac ggcgccagca
     1261 gatattttgt tcgcggttac tgcatttgca gcgatcttgt ttgcggtcac agcattggcc
     1321 gctatcttgt cggcctcaat tgcccccgct tttattttag gcgtgcttat ggcgttgtca
     1381 cttatcttag tttcggtaat ggctcccgta ttaatctttg tggtagttac
//
LOCUS       DASRMS010000013         8677 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_538910, whole genome shotgun sequence.
ACCESSION   DASRMS010000013 DASRMS010000000
VERSION     DASRMS010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 8677)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8677)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8677
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_538910"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..1077
                     /locus_tag="VFC74_00840"
     CDS             103..1077
                     /locus_tag="VFC74_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZK23916.1"
                     /translation="MRFNSFKRYLQNERGTAIVILAGAMTLLMACTALVADIGVNFVT
                     QSRLAVAADAAALAGATKFQDGTAQVQETALAIAAQNGIKAEDVSVNVAGSGQEVTVT
                     ATAPIKLFFAKLFSKEDGEMSQSARVIAGRPTAMDSIVPLGINEHASFEFNETYKLFA
                     KNANTDELELGSGNSGALAFREGAEGANEFEYYLRKGYHEVISLGDILYTESGVKYSA
                     LKSGFAARLSAAQADSSHTCGFATCPSSCPRIIYIPVYRSLWENGKVTRVEVVDFAAF
                     WLEAKQLGSGEWKIDKEITGRFIRRSKGGFTTEEGESPWGLSSGKLVE"
     gene            1200..1901
                     /gene="cpaB"
                     /locus_tag="VFC74_00845"
     CDS             1200..1901
                     /gene="cpaB"
                     /locus_tag="VFC74_00845"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HZK23917.1"
                     /translation="MKGKKLLLLSLVLALAAAGAIYTYLNQLEAKSKAAAALVTVLVA
                     KEEIPARVKLDNSMFTTVELPKAALHTDAVLDAAEVAGTFSRERLLAGEQVLSSRLVF
                     EQSKDGLAYQISTNHRALTVPVNNVSGVAGYILPGDFVDAIVTIDPASANEGETLTKV
                     VAAKVRVLAAGQYLREQATEQLVVDTVTLDAPQDAVTALIQASERGSLRLVLRPVSDT
                     STTVMPAYSIGQFHQ"
     gene            1939..3216
                     /locus_tag="VFC74_00850"
     CDS             1939..3216
                     /locus_tag="VFC74_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK23918.1"
                     /translation="MEKIKIMIVDDIPQTRKDLIRLLYFEEDMKVVGEAENGQEALEK
                     MGELQPDVVLMDINMPQMDGITATEKACQLFPQVAVVIISIQGESEYLKKAMVAGARD
                     YLVKPLSSDEMGLTIRTVYKQQQLRRSSQYEIEPLPPLLRHDQAEAEKRAEGSTKPAI
                     AEEKSDGKVVVVFAGKGGCGKTTIAINLAVALAQNQKKRVALVDYDLQFGDIAVMLNL
                     SDGHNLSQLVQSTESITEADLAGYLIRHFSGIDILPAPLFPQDAEYVTTEHADNILQL
                     LKLQYDYIIVDTASLFNDVNLQVLELAEQIMLVVTRDIPTIKNAKTSLNILETLNYRD
                     KIRVVLNRSNQDLGVEVPDLETGLEITVSHQITSDEKAVISAINKGAPVTVSHAAAEI
                     SKDFRRLGERLVNGQRLGGAEKQKGIITRIFSL"
     gene            3230..4603
                     /locus_tag="VFC74_00855"
     CDS             3230..4603
                     /locus_tag="VFC74_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HZK23919.1"
                     /translation="MSLLERLERIKLQERQVQEQKPHLVTIEKKTEPVPKQDPYTKLK
                     IKIHKKVIEELGQQMEGEGDDALPEHIEKLVAAAIDAEVNFIPIGDKQRITTEIVAEA
                     VGFGPIHPLLNDETVSEVMVNGPNQVYVERHGKLELTDVVFRNDEHVLHIIEKIVAPL
                     GRRIDESSPMVDARLPDGSRVNAVIPPISLVGPSITIRKFAKDPFTIDDLINFDTLNL
                     EMAAFLESAVKARLNVVVSGGTGSGKTSTLNVLSSFIPHGERIVTIEDAAELQLGQDH
                     VVSLETRPANVEGKGAISIRDLVRNSLRMRPERIIVGEVRSGEALDMLQAMNTGHDGS
                     LTTGHANSPRDMLSRLETMVLMAGMELPVRAIREQIASAIDIIVQQSRLRDGSRKITH
                     ITEVQGMEGDVIVLQDLFVFEQSGLDGNGKCIGTYRSTGIRPKAIQKMEAAGIALPAA
                     MFASARF"
     gene            4618..5580
                     /locus_tag="VFC74_00860"
     CDS             4618..5580
                     /locus_tag="VFC74_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZK23920.1"
                     /translation="MQQLKIALLTFLACFFTIYSLTTQREQEKVEVKQRVWQYTGNRE
                     NNEEKEEAKPQKSLLKRCLTLFSLLMPKRLLKNVEADLVQADIPLKVEEMVGLNIALA
                     LLPAILTMLATHDSLLALLLACVGAYLPRLLIKSAKAKRAQKFNDQLGDALQLMSNSL
                     RAGFSFLQAMDSISKEMAAPISHEFARTMREMRLGTTTEEALHNLGTRIRSDDLDLVI
                     TAVSIQRQVGGNLAQILENISTTISERIRMKGEVKTLTSQGRISGTIVALLPVFLVIA
                     IGLMNPSYIMALFTHPIGLVITGGAVISEIVGIMAIKKIINIEF"
     gene            5594..6511
                     /locus_tag="VFC74_00865"
     CDS             5594..6511
                     /locus_tag="VFC74_00865"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZK23921.1"
                     /translation="MLLASTGLVFLTIYTFFSAIRSRQTQEKADLQGRLKNALEWEEP
                     ADVREAELSRPFLERLSAPLLQAVSTLASRLLPAIILQSLEKKVQQTGRQGGLSPRNY
                     LGMKLLAVVTVPVVIYLLMGSTWSLKTLGLVAFSAFIAWRLPEMQLDRIIRQRHDQLE
                     KSFPDSMDLLTVSVEAGLGFDGALAKVAEKSEGPVAEEFRRLLHEIRMGKSRKEALRD
                     FGVRCGVEDIILFTSTVIQAEQLGLNISNTLKTQAEQMRRKRRQRVEEKAMKIPVKML
                     MPMVVFIFPTIFVVLLGPALIQIMENFVR"
     gene            6517..6867
                     /locus_tag="VFC74_00870"
     CDS             6517..6867
                     /locus_tag="VFC74_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HZK23922.1"
                     /translation="MLLRTRDQTLIAQHVYSATTFLRRLRGLMFSKCFPQGYDALLLS
                     PCNAVHTMFMHYTIDVVFLDQAWQVVAIQPFMQPWQLSAIVKKADHVLELPAGTINRL
                     KLTPGDYLAELPAK"
     gene            6933..7475
                     /locus_tag="VFC74_00875"
     CDS             6933..7475
                     /locus_tag="VFC74_00875"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZK23923.1"
                     /translation="MQFFVLSIFVIICLVTDLRERKIYNKVIIAGLVSALVINIILQG
                     FVNGLRFTVVGLLMGIILLLLPFIMGGLGAGDVKMLGMIGAFTGSSMVVQILLISALV
                     GGVFALITMAKEGRILMRLAQLFKGLYCFIFTRKSLHLGGLHETEASTKSIPYGVALS
                     VGVLIVYILGSMEYVLPGFS"
     gene            complement(7595..8650)
                     /locus_tag="VFC74_00880"
     CDS             complement(7595..8650)
                     /locus_tag="VFC74_00880"
                     /inference="COORDINATES: protein motif:HMM:NF017847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HZK23924.1"
                     /translation="MKIGIIGPALAGKTTLFQLLTGATSPQGGKGGIPQGTARIPDAR
                     IDKLSAIFKPKKTIHATVEFADFPALGHGGELSGETASRLKALDALAVVVRSHRDPAV
                     PWPEEPVTPDIAFSNFLQEMILTDLVQVEKLLSRNKDKKRTADEDQLLIDCQALLEDM
                     KPISAGTWGEEALPFLRNYAFLSSRPVLVAVNVDEEQLQEHSYEGQQNLTQQCHAAGY
                     PLIEFCGTLEREISQMELSEQQVFMAEYGLTQSGIARLAAAAYQLLDLISFFTVGEDE
                     VRAWTIDATTPAKKAAGKIHSDIERGFIRAEVIHYEEFMGLGASLKTAREQGKLRLEG
                     KEYLVQDGDIINFRFNV"
BASE COUNT         2368 a         1766 c         2183 g         2360 t
ORIGIN      
        1 gctacccggt gcagttaatc acgcccttag ctggcagagt ggttagtaat ccttggcttg
       61 tcaaaagcgc catcaccatg cgtgtggaat gaggtgaaag gcatgcgctt taatagtttt
      121 aaaagatatt tgcagaatga acgtggtacc gccatcgtga tcttggctgg ggcgatgacg
      181 ctgttgatgg cgtgcaccgc tttagtggca gatattggcg ttaattttgt tacccagtcc
      241 cgtctggcgg tggcagctga tgctgctgcc ctggctggag caactaaatt tcaggatggg
      301 acagcgcaag tgcaggaaac ggcactggcc atagccgcac aaaatggcat caaggctgag
      361 gatgttagcg ttaatgtggc tggcagtggg caggaagtga ctgtcaccgc aaccgcaccc
      421 atcaaattgt tctttgctaa gctatttagt aaggaagatg gtgagatgtc gcaaagtgcc
      481 cgcgtgattg cggggcgtcc aactgccatg gatagtattg ttccactggg gattaacgaa
      541 catgcaagtt ttgagtttaa tgaaacctat aaattatttg ccaagaatgc caatacagac
      601 gagttagagt tgggatcagg caattccgga gcgctggctt ttagagaagg tgccgaaggt
      661 gctaatgaat ttgaatatta tctgcgcaag ggttatcatg aggtaatttc cttaggggat
      721 attctctaca cagaatcagg tgtaaaatac tctgctttga aaagtggttt tgctgcccgt
      781 ttgagtgcgg cccaagctga cagtagtcac acctgtggtt ttgccacctg tccatctagc
      841 tgcccgcgca ttatttatat acctgtttat cggtctcttt gggaaaacgg taaagttacc
      901 agggttgaag tggtagattt cgcggccttt tggctggagg caaaacagct gggcagtggt
      961 gaatggaaaa ttgataaaga aattaccggt aggtttattc gcaggtctaa aggcggcttt
     1021 accacagaag aaggggaaag tccctggggc ctgagcagcg gcaaattagt agaataaaaa
     1081 ataatcaagc ttccagcggc agatactagg gtattaaagc tcccccaaaa gcgcccctgg
     1141 ttatccgccc tggcttattt agtactggct atgaacagcg aagtagatga ggtgaagcca
     1201 tgaaggggaa aaagttatta ttgttatcac tggtattggc actggctgcg gcaggcgcca
     1261 tctatacata tttaaatcaa ctagaagcga aaagtaaagc tgccgcagca ctggtaacgg
     1321 tgctggtggc caaagaagaa ataccggcaa gggttaagct ggataactcc atgtttacca
     1381 ctgttgagct gccgaaagct gctctgcata ccgacgcggt gctagatgcc gcagaagtag
     1441 cagggacatt tagccgtgaa cggttgctgg ccggagaaca agtattgtct tcgcgcctgg
     1501 tgtttgagca aagcaaggat ggtctggctt atcaaatttc tactaatcac cgtgctctga
     1561 cggtgccggt taataatgtt tctggggtag ccggatacat tttgcccggg gattttgtcg
     1621 atgccattgt cactatcgat cctgcttctg ctaatgaggg agaaacgctg accaaagtgg
     1681 ttgccgccaa ggtaagagtg ttggcagcag gccaatattt gcgcgaacaa gcaacagaac
     1741 agttagtggt ggatacggtg accttagatg ccccgcagga cgcggttacc gctttaattc
     1801 aagcttccga acgcggcagt ttacgtttgg tgctgcgccc tgttagtgat accagtacca
     1861 ctgtaatgcc tgcatatagc attggacaat ttcaccagta gtctgagtct ggtggtatta
     1921 aattagggag ggatcaacgt ggaaaaaata aaaataatga tcgtagacga tatccctcag
     1981 acgcgtaaag atctaattcg gctactttat tttgaggaag atatgaaagt ggttggagaa
     2041 gcagaaaacg ggcaagaagc tctggagaaa atgggagagt tacagccaga tgtagtttta
     2101 atggatatta atatgccgca aatggatggc attacggcca cggaaaaggc ctgccagctt
     2161 tttccccaag tggcggtggt gattatttcc attcagggag aatctgaata tttgaaaaaa
     2221 gcgatggtgg ccggagcaag agactatctg gttaaaccac tgtccagtga tgaaatgggc
     2281 ctgacgatac gcactgtcta taaacagcag cagctgcggc ggtctagtca atatgaaatt
     2341 gaacctttac cgcccttact gaggcatgac caagcagagg cagaaaagag agcagaaggc
     2401 agcaccaaac cggcaatagc agaagaaaaa tcagatggta aagtggtggt agtctttgct
     2461 ggcaagggtg gctgtggtaa aaccaccatt gctatcaatt tggccgtggc tttagcgcaa
     2521 aaccaaaaga agcgggtagc tttggtggat tatgatctgc agtttggtga cattgcggtg
     2581 atgctgaact tgagtgatgg ccataacctt agccaactcg tccagagtac ggaaagtatt
     2641 acggaagcag acttggcggg ctatttaatt aggcattttt ctggtattga tattttaccg
     2701 gcgccgttat ttccccaaga tgccgaatat gtgaccacag agcatgcaga taacattctc
     2761 cagctgctaa aactacagta tgattacata attgttgata ccgcatctct gtttaatgat
     2821 gttaatctgc aggtactgga gttggctgaa caaattatgc tggtcgtaac gcgtgatatc
     2881 cccaccatta agaatgctaa aaccagctta aatattttag aaacgctaaa ttatcgtgat
     2941 aagattcggg tcgtcttaaa tcgcagtaat caagatttgg gcgtggaagt gcctgattta
     3001 gaaacaggtt tagagatcac tgtttcgcac caaattacta gcgatgaaaa agcggtgatc
     3061 tccgccatca ataagggtgc gccggtgact gtcagccatg ctgccgcgga aattagcaaa
     3121 gacttccgtc gcttaggcga gcgtcttgtt aatgggcagc gtttaggcgg tgccgaaaaa
     3181 caaaaaggga ttattacccg tatctttagc ttataggggg gtgtggtaaa tgtctttact
     3241 ggaacgatta gaacgaataa aattgcagga acgccaagtg caggaacaaa agccacattt
     3301 agtaacaata gagaaaaaaa cggagcccgt gcctaaacag gatccctata caaaacttaa
     3361 aataaagatt cataaaaaag tgatcgagga actgggacaa caaatggaag gtgagggcga
     3421 cgatgcttta ccggagcaca ttgaaaagtt agtagctgcg gcaattgatg cggaagttaa
     3481 ttttataccc atcggtgata aacaacgcat cactactgaa atcgtggctg aggcggtcgg
     3541 ctttggtccc attcatccct tattaaatga tgaaactgtt tctgaagtaa tggttaatgg
     3601 tccgaaccaa gtctatgtcg agcgccatgg taagttagaa ttaactgatg tagtttttcg
     3661 taacgatgaa catgttctgc atattattga aaaaatagta gcacccctgg gcagacgaat
     3721 tgatgaaagt tctcccatgg ttgatgcgcg cttaccagac ggttcacggg tgaatgctgt
     3781 tatcccgcct atttctctgg tggggcccag tatcaccatt cgtaaatttg ctaaagaccc
     3841 ttttaccatt gacgacttaa taaattttga cactttaaat ttagaaatgg ccgccttttt
     3901 agagtcagcc gtcaaagccc gcttaaatgt ggtggtctca gggggtaccg gttctggtaa
     3961 aacttctact ctcaatgttt tgtcttcatt tattccccac ggggaaagaa ttgtcaccat
     4021 tgaagatgcg gcagaactac agttaggcca ggaccacgta gtctctttgg aaaccaggcc
     4081 tgccaatgtc gaaggcaaag gggcgatcag catccgagat ttagtgcgta actctctgcg
     4141 gatgcgtccg gaacgtatta ttgtcggtga ggtgcgcagc ggcgaagctc tagatatgct
     4201 gcaggcgatg aataccggtc atgatggttc actgactaca ggtcatgcca attcaccgcg
     4261 agatatgttg tcacggctgg agaccatggt cttgatggcc ggcatggaat tgccagtgcg
     4321 agcgattcgt gaacaaattg cctcagcgat agatattatt gtacagcaga gccgtctgcg
     4381 ggacggcagc cgtaaaatta cccatattac cgaagtacaa ggcatggaag gcgatgtaat
     4441 tgtcctgcag gatctatttg tctttgaaca aagtggtctt gatggcaacg gtaaatgtat
     4501 cggtacttat cgttctacag gtattcggcc taaagctatc caaaagatgg aggcggcggg
     4561 aattgctctg cccgcagcca tgtttgcttc ggctaggttt taaaggagtt gtgaagtatg
     4621 cagcagctaa aaattgcgct attaactttc ttggcctgtt tttttacaat ctatagtttg
     4681 acgacacagc gagagcagga aaaggttgaa gttaaacagc gtgtctggca gtatacaggg
     4741 aatagagaaa ataatgagga aaaagaggaa gctaaaccgc aaaaatcttt gcttaaacgc
     4801 tgcttgacgc tgtttagttt gctgatgccc aagcgcctgt tgaagaatgt tgaagctgat
     4861 cttgtccagg ctgatatccc gcttaaggta gaagagatgg tgggtttaaa tattgcttta
     4921 gccttactac cagcgatttt aaccatgctc gcgacccatg atagtctact tgctttactt
     4981 ttggcttgtg taggagcata tctgccgcgg ctgctaatta aaagtgccaa agctaagcgg
     5041 gcgcaaaaat tcaatgatca gttaggtgat gccttacagc taatgtctaa ttcactgcgc
     5101 gccggttttt cttttttgca ggcgatggac tccattagta aagagatggc agcaccaata
     5161 tcgcatgaat ttgcccgcac gatgcgtgaa atgcgtttag gtacaacaac tgaagaagca
     5221 ctgcataatc tggggacacg cattagaagt gacgacttgg atttagttat cacggcagtg
     5281 tctattcaaa gacaggtggg aggtaatctc gcccagatac tagaaaacat ctccactacc
     5341 atcagcgaac gcattcgcat gaagggggaa gtaaaaacat taacgtcgca aggcagaatt
     5401 tctggtacaa ttgtcgctct gttaccagtc tttctggtga ttgctatcgg cctgatgaat
     5461 cccagctaca ttatggcttt gtttacccac cctataggat tggtgataac tggcggcgct
     5521 gtaatttcgg agattgttgg cattatggcc attaagaaaa taattaatat cgaattttaa
     5581 ggggggagca gtgatgctgc tagcaagtac cggtttggtt ttcctcacaa tctatacttt
     5641 tttttctgcc atccgtagtc ggcaaacaca ggaaaaagca gatttacaag ggcgcttaaa
     5701 aaatgccttg gaatgggagg aaccagcaga tgtcagggag gcggagttaa gcagaccttt
     5761 cttagaacgg ttatctgctc ctcttttaca agctgtttct actttggctt cacgcctttt
     5821 gcctgctata attctccagt cgttagaaaa gaaagtacag caaacaggcc ggcagggagg
     5881 tttaagccct cgcaattatc ttggcatgaa attactggcg gtagttactg taccagttgt
     5941 catctatttg ctgatgggca gtacttggag tctgaaaact cttggcttgg tcgcttttag
     6001 tgcttttatc gcctggcgtc tgccggaaat gcagttagac cggatcattc ggcagcggca
     6061 cgaccaactg gaaaaaagct ttcctgattc tatggattta ttaacggtga gtgtagaagc
     6121 tggtttaggt tttgatggag cactggcgaa agtggctgaa aaaagtgaag gacctgtggc
     6181 tgaagaattt cgcagattgt tacatgaaat aaggatggga aaatcacgca aagaagcgct
     6241 gcgtgacttt ggggtacgct gtggggtgga agatattatt ctctttacca gtacagtgat
     6301 acaggcagag cagttaggtc tgaatattag taatacctta aaaacacaag cagaacaaat
     6361 gcggcgcaaa agaaggcagc gggttgaaga aaaggcaatg aaaataccgg ttaaaatgtt
     6421 aatgcccatg gttgtcttta tctttcccac catctttgta gttttgttag gtccggcgct
     6481 cattcaaatt atggagaact ttgttaggtg agtccgatgt tactacgcac aagagaccaa
     6541 acgctcattg ctcagcatgt ttattctgcc actacatttt taagacgctt acgaggcttg
     6601 atgtttagca agtgctttcc gcaaggttat gacgccttac ttctttcacc gtgtaatgct
     6661 gtgcacacga tgtttatgca ctacactatt gatgtagtct ttttagacca agcatggcag
     6721 gtggtggcca tacagccttt tatgcagccc tggcagcttt ccgctattgt taaaaaagcc
     6781 gaccatgttt tggaactgcc tgctggcacc ataaaccgtt taaagcttac cccaggcgat
     6841 tatttagctg aactgccagc caagtgagtt gggctgaccg gaaattaaga tggagggaat
     6901 aggtcttgac cgttccgcaa ggaggtgcag caatgcaatt tttcgtactt agtatctttg
     6961 tcatcatctg tttagtcaca gatttgcggg aaagaaaaat ctataataaa gtaattatcg
     7021 ctggtttggt tagtgcacta gtaattaata ttattttaca ggggtttgtc aacgggctcc
     7081 gcttcactgt cgtaggttta ctgatgggta taattttatt gcttcttcct tttatcatgg
     7141 gtggtttagg cgctggagat gtaaaaatgc tgggcatgat cggtgctttt accggttcat
     7201 caatggtagt gcaaatttta ctaatctcag ctttagttgg tggtgtattt gccttgatta
     7261 caatggcgaa agaaggtcga atactaatgc gtctggcgca gttgtttaaa ggtctttatt
     7321 gttttatttt tacccgcaaa agtttgcatt taggtggctt acacgaaact gaagcttcca
     7381 caaaaagcat tccctatgga gtagctcttt cagtaggggt gttgattgta tatattctgg
     7441 ggtcaatgga gtatgtactg ccgggattta gttaagcaaa aaggaaagct aaaactgttt
     7501 cccagtataa aaaaacggag tacctgtcaa acaggtgctc cgtttttgcg tatttagtta
     7561 tcctcaacag cttctgttgt cgtgaaagat ctggttagac attaaagcga aagttaataa
     7621 tatccccatc ttgtaccaga tactctttac cttcaaggcg tagtttccct tgttcacggg
     7681 cagtttttaa ggaggcgccc agtcccataa attcttcgta atgaataact tcggcgcgta
     7741 taaaaccacg ctcaatatcg gaatgaattt tacctgctgc ttttttagcc ggcgtagtag
     7801 catcaatagt ccaggcccgt acttcatctt caccaacagt aaagaaagaa ataaggtcca
     7861 gcagctgata agctgctgct gccaagcggg cgataccaga ctgggtcaga ccgtactccg
     7921 ccataaaaac ttgctgttct gacagctcca tttgactaat ttccctttct aaggtgccac
     7981 aaaattctat caaggggtag ccggctgcgt ggcactgttg agttagattc tgctgcccct
     8041 cgtagctatg ttcctgcaat tgttcttcat ctacattaac ggctaccaga accggccttg
     8101 atgaaagaaa ggcataattg cgcagaaagg gcagcgcttc ttcgccccag gtgcccgcgg
     8161 aaatcggctt catatcctct aacagggctt gacaatcaat taataactgg tcttcgtcag
     8221 cagtacgttt tttatcctta ttacgggaaa gcagtttttc cacctgcact aaatcagtga
     8281 gaatcatctc ctgcaggaag tttgaaaagg cgatatcggg agtaactggc tcctcgggcc
     8341 aaggtacagc aggatctcgg tggctccgca ctactaccgc taaggcatct aaagctttca
     8401 agcgactagc agtttcgccg cttaattcgc cgccgtgccc tagcgcgggg aaatccgcaa
     8461 actccacggt agcatgaatt gtttttttag gcttaaaaat agcagataat ttatcaatgc
     8521 gggcatcagg aatccgagct gttccttgcg gaatcccgcc tttaccgcct tgaggggagg
     8581 ttgctcccgt cagtaattga aatagagttg ttttgccggc taaggccggg ccaataatgc
     8641 caattttcat cgtattttac acctcacgct accaatc
//
LOCUS       DASRMS010000014        13211 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_874624, whole genome shotgun sequence.
ACCESSION   DASRMS010000014 DASRMS010000000
VERSION     DASRMS010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 13211)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13211)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13211
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874624"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1667)
                     /locus_tag="VFC74_00885"
     CDS             complement(159..1667)
                     /locus_tag="VFC74_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HZK23925.1"
                     /translation="MLARVTGCTIVGIEGRMVDVEVYLSSQLPSFDIVGLPDTAVREA
                     RDRVRAAIKNSSLQFPQQRIIVNLAPADLKKEGPQLDLALAMGILQAYGQLQLPDVPW
                     AFLGELALDGTLRGVRGVLPMVMAARHAGCRNILLPLANAGEAALVSGVHIIGATSLR
                     EALAILAGSQLPPPLPQSKQLPLQNGADFKQVKGQEGAKRALEVAAAGGHSLLMSGPP
                     GSGKTMLARCVPSVLPPMTEEESLQTTTIYSAAGLYKENCGLMSSRPFRSPHHTITPA
                     ALCGGGSHPRPGEVSLAHQGVLFLDELPEFRRETLEVLRQPLEEGFVTVTRLMASYTF
                     PSRFMLLASMNPCPCGMLADAETECVCSERQIHRYRHKISGPLLDRMDLFVEVTRLRY
                     DEVAGHGTGEASAAIAKRVAAARKLQQQRLQAAGLSCNAQMAPQHIQKFCETDAAGRD
                     LLRHAFSRLHLSARAYDRILKVARTIADLEAEQKIHTRHLAEAIGYRQLVNF"
     gene            1776..3911
                     /locus_tag="VFC74_00890"
     CDS             1776..3911
                     /locus_tag="VFC74_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZK23926.1"
                     /translation="MKKTLIIILLLGLSALLGFYYLQQTKDELADNTRLFFAAAVIGS
                     EQAQDYLAPNIENKEALLTALQTEALITKAEIRQVRLHSYKRASVLVDLEINKKVTSL
                     EAQFIREGRTWRINSLPAFTVLEQALISNLDADQVTLFTTQGKHTFVIQADDAVQSGN
                     IGDAVAIERNLIYFKAYAAEETAKLLAITSDALEVEMSGLHPLAADVVYFQPQQNSFR
                     LVDKQSLLVGMEDLTLYWQDNEVKAVMLPENFLPTNIRVLLNTTGFTSISHRQVTLSA
                     NTSYLLTDKAAKQQYRFTSGQKLSFKPTDNGIEATFPDSRKQSFPNRLYLIPDHHGQL
                     RVETLKRGTPAFTPTYHGHLEIKAAQGALLLVNEVPLETYLYSVVPSEMPISFGAVPL
                     QVQAVAARTYAVAAMYRGGFKQYGAHVDDSVSSQVYNNVPEYAASTAAVNHTQGVIVK
                     YQDTIADTRFFSTSAGVTANKNEVWHDEASGSFPGTAVPYLRALSQLRTGQLPDVTSE
                     AGAREFFTQSNRDSYDQNSPWFRWQVEMTAAELTAVLKQYLPERYRAQPDFVLTKAGD
                     DFVSKEVAAEPLGKLTDLRVIRRGAGGNILELELVGEKGTYRLLKEYTIRFTLRPISM
                     GSGKDVILTRHDGSTLANYTILPSTFMVFEVKRDSSNNITSILFQGGGNGHGVGLSQW
                     GSRGLAEDGRSFSEILQHYYPGCTLTNIY"
     gene            4120..4557
                     /locus_tag="VFC74_00895"
     CDS             4120..4557
                     /locus_tag="VFC74_00895"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HZK23927.1"
                     /translation="MLKQIQDDMKTALKAGEKKKVSTLRMLIADIKNESINKRSALST
                     DEILSVIQREIKQRRNANEEFKKGNRLDLVAENEIEVAILTAYLPQQLGDEELETIVL
                     QAVSAVSATSLKEMGKVMSKVMPQVKGKADGRRVQAAVKKVLS"
     gene            complement(4598..5362)
                     /locus_tag="VFC74_00900"
     CDS             complement(4598..5362)
                     /locus_tag="VFC74_00900"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HZK23928.1"
                     /translation="MNKKLLTIMITTCFLMTLITGAAAQTLAFGARELQIGDRGEDVA
                     VMQEFLNQHGYREGHLDGVFGPMTYGSLKAFQKEQKLKTDGVLAEKTLTRMQEMMSEK
                     PAATVKAKSMAAPAKVETTQTLVAVNNHNFTEADLELFARLVHAESAGEPFDGQVAVA
                     ATILNRVENERYPNTISGVVYQVESGRYQYSPVLDGRINQPASESAKRAVREALKGLD
                     PTAGATGFYNPAKTSNAWVRSHPVTTSIGSHVFFKY"
     gene            complement(5754..6092)
                     /locus_tag="VFC74_00905"
     CDS             complement(5754..6092)
                     /locus_tag="VFC74_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HZK23929.1"
                     /translation="MKALGRRGEELAAAYLTKHGYQVLVQNWRCERGELDLVARDGQL
                     LVFIEVKTRRSQRHGTPAEAVDARKQEKIRHLALQYIHLTGNTAPAYRFDVVTVDGVS
                     EQVTLIKNAF"
     gene            complement(6089..6871)
                     /locus_tag="VFC74_00910"
     CDS             complement(6089..6871)
                     /locus_tag="VFC74_00910"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HZK23930.1"
                     /translation="MSNFTKMTVAQIRRYFKETVPTPAELAQLSADQRVGVRHVVQSY
                     LHQLEAQRLEKERLSALHKFERKARLAGYTYIAGVDEAGRGPLAGPVVAAAVILPENF
                     SVTGLNDSKKVSPLNREKLYTIITKNAIAWSVGAGQVAEIDQYNILNATKMAMRRAIL
                     GLTTKPDYVLLDALELEDLPYPQQGIIGGDGLSASIAAASIIAKVTRDRLMCELAELL
                     PGYGFAEHKGYPTREHRKAIAQLGATPFHRQSFMLLPAEEER"
     gene            complement(6822..7703)
                     /gene="ylqF"
                     /locus_tag="VFC74_00915"
     CDS             complement(6822..7703)
                     /gene="ylqF"
                     /locus_tag="VFC74_00915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HZK23931.1"
                     /translation="MKIQWYPGQMAKAKRLLRENLKLVDAVIEVLDSRIPYSSRNPDI
                     RDLLARRPVVAALTRADLANPQVTSAWVQALRENYFAVAALNANSGQGVKNLLAILKT
                     IPQRRAQRPLRLMVVGIPNVGKSSLINRLIGRSAAMTGDKPGVTKGKQWLRLAANLEL
                     LDTPGLLWPKITSKRQAFSLAAVGAIKDEIYDPVELALELMTYLLANVPQNLQQRYKL
                     GSVSGETRLLLEEIGKRRGCLVKGGEVDLEQAARLLIKDFREARLGRLTLEQVRELGE
                     NNEQFHENDSGADSPLL"
     gene            complement(7721..8245)
                     /gene="lepB"
                     /locus_tag="VFC74_00920"
     CDS             complement(7721..8245)
                     /gene="lepB"
                     /locus_tag="VFC74_00920"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK23932.1"
                     /translation="MQEADKNEMWEWIKSILLAIVLALVIRAFLLEVFLVKGDSMSPT
                     LHNRERILVSKIQYYYREPQPGEIVVFQATEDRDFIKRIIATAGDEVVINPDGVFVNG
                     EKITETYIADQAREPYGPATVPEGTFFVMGDNRNNSMDSRHPAVSFVEMAEIKGKAMF
                     IFWPLDNIRKISHP"
     gene            complement(8446..8790)
                     /gene="rplS"
                     /locus_tag="VFC74_00925"
     CDS             complement(8446..8790)
                     /gene="rplS"
                     /locus_tag="VFC74_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HZK23933.1"
                     /translation="MDVIRQIEAEQMKKDLPSISPGDTVRVHVKIVEGTRERTQIFEG
                     VVIKRQGSGLRETFTVRRISYNVGVERTFPLHSPKLEKIEVVRHGIVRRAKLYYLRKL
                     TGKAARIKERRY"
     gene            complement(8952..9698)
                     /gene="trmD"
                     /locus_tag="VFC74_00930"
     CDS             complement(8952..9698)
                     /gene="trmD"
                     /locus_tag="VFC74_00930"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HZK23934.1"
                     /translation="MRIDILTLFPNMFASLTESIIKRAAEDGQVKIVITDIRDFAQNK
                     HRNVDDYPYGGGPGMVMQAEPFFLALEHLRQNSKQRGRVILMSPQGQPFSQQKARQLA
                     QEERLFLLCGHYEGIDERVKDALVDEEISLGDFVLTGGEIPAMAIADAVVRLLPGVLP
                     AASTAEESFTDNLLEYPQYTRPAEYQGLAVPEILLSGNHERIKQWRRKQALLRTLISR
                     PDLLSKAELTAEEKQFLAAEAKRRQLDDLL"
     gene            complement(9708..10205)
                     /gene="rimM"
                     /locus_tag="VFC74_00935"
     CDS             complement(9708..10205)
                     /gene="rimM"
                     /locus_tag="VFC74_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HZK23935.1"
                     /translation="MATIGRVVNTHGVKGGLKILPLSDFLERVKSLKRVFMEHGERVS
                     AYQVEDAFVHGRFWVVFLQDVEDMDSAERFKGALLKIPLTERVELPAGSYYLDQIIGL
                     TVFTLAGLELGQVSDVLQTGSNDVYVVRQSEGPEILIPALKTVVKKIDLQHARLEVDL
                     PEGLM"
     gene            complement(10226..10456)
                     /locus_tag="VFC74_00940"
     CDS             complement(10226..10456)
                     /locus_tag="VFC74_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012742360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HZK23936.1"
                     /translation="MKDLVEFIAKALVDNPDEVRVEQLEDERAITLELHVAADDMGKV
                     IGKQGRIAKAIRTVVNAAATTERKRVFVEIVQ"
     gene            complement(10481..10726)
                     /gene="rpsP"
                     /locus_tag="VFC74_00945"
     CDS             complement(10481..10726)
                     /gene="rpsP"
                     /locus_tag="VFC74_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HZK23937.1"
                     /translation="MAVRIRLKRVGAKKRPFYRIVVADSRSPRDGKFIDEIGVYNPTT
                     EPVTLKIDDEKAQTWLQKGAQPSDTVKSLLTKAGIGK"
     gene            complement(10761..12107)
                     /gene="ffh"
                     /locus_tag="VFC74_00950"
     CDS             complement(10761..12107)
                     /gene="ffh"
                     /locus_tag="VFC74_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HZK23938.1"
                     /translation="MIFENLSNKLQDTFKRLRGKGKLTEKDVTAALREVRLALLEADV
                     NFKVVKDFVNTVKERSLGSDVLASLTPGQMVIKIVNEEMTKLMGETAAKLNQANQPPT
                     VIMMVGLQGAGKTTSAAKLANLLKKKNHTPLLVAADIYRPAAIKQLQVLGEQLAVPVF
                     QMGQQDPVQTAKAALEHAKKSRHDYVIVDTAGRLHVDEELMNELVSMEEAAQPQEILL
                     VVDAMTGQDAVNVAQSFKERLGLTGVVLTKLDGDTRGGAALSVRAVTGAPIKFAATGE
                     KIDAFETFHPDRMASRILGMGDVLSLIEKAQASFDEDKARELEQKIRSQQFTLEDFLD
                     QMQQLKKMGPLNQIMEMVPGMGGMNKKLKGLDVDEKQFARTEAIIKSMTKKERLNPTI
                     INSSRRKRIAAGSGTTVQDVNSLLKQFNTMQKMMKQFSKLDKGKLKGKSKGKFPFM"
     gene            complement(12128..12463)
                     /locus_tag="VFC74_00955"
     CDS             complement(12128..12463)
                     /locus_tag="VFC74_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009321389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxM family DNA-binding protein"
                     /protein_id="HZK23939.1"
                     /translation="MLEDTTRVNLLYDFYGNLLTKKQRAYLELYYQDDLSLAEIADNE
                     GISRQGVHDLVKRAVRSLENAEKRLGLLVRFTAQEKELQRLREILTKSEMPEQNRREA
                     LTIVEKLLD"
     gene            complement(12570..>13211)
                     /locus_tag="VFC74_00960"
     CDS             complement(12570..>13211)
                     /locus_tag="VFC74_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/oligopeptide/nickel ABC transporter
                     ATP-binding protein"
                     /protein_id="HZK23940.1"
                     /translation="DLNLEVAAGEVVSLIGESGIGKSTLAQILAGFLASEAGEVYFAG
                     EKANFKQLHRQQGGLQMVFQDPQAALNPNFTVQSAVAEPLLLNHTKMDLSAAVKSALL
                     DVGLPQTAEFLAKKVRQLSGGQKQRLALARALVMAPKLLIADEPTSMLDASSKANLLR
                     LLKGLQNAKGFSLLMVTHDLASAVKVSNRIYVLEDQVRLTEFTDLTRFACGQG"
BASE COUNT         3549 a         3259 c         2767 g         3636 t
ORIGIN      
        1 atcttgaatt aagtcataaa acctatcggg ttgtcggctc gccggatctc actgccctct
       61 atgagctgat agtttcagcc caaccggcag gatcactaga ccaattttac tacgaactga
      121 ttaagaaaaa tgaataaaac aacttctgca cccagcagct aaaagttcac aagctgacgg
      181 taaccaatgg cttctgccag gtgtcgcgtg tggatttttt gctccgcttc taaatcagcg
      241 atggtacgag caaccttgag aatacggtca taggcgcggg cacttaaatg aagtcgtgaa
      301 aaggcgtgac gcagcaggtc gcgcccggcg gcatcggtct cacagaattt ctggatatgc
      361 tgcggtgcca tttgcgcatt acaggacaat ccagcggcct gcaggcgctg ctgctgcagc
      421 ttacgtgctg cagctacgcg tttagcaatg gcagcagaag cctcaccagt gccgtggcct
      481 gccacttcat cataacgcag gcgcgtcacc tcaacaaata agtccatgcg atccaacaag
      541 ggaccagaga ttttatgtcg gtagcgatga atttgccgct cggagcaaac acattccgtt
      601 tcagcatcag caagcatacc gcagggacag gggttcatcg aggccaagag cataaaacgg
      661 gagggaaaag tataagacgc cattagtctg gtcacagtaa caaagccttc ttccagcggt
      721 tggcgtaaga cttctaatgt ttcgcggcga aactccggca gttcatctag aaataagacg
      781 ccctgatgcg ctaaactaac ttcaccggga cggggatggc ttcctccccc acacaaggcg
      841 gcaggtgtaa tcgtatgatg aggactgcga aaaggtctgc tgctcattaa tccgcagttt
      901 tctttataca gacctgcagc actataaatt gtcgtagttt gcagactttc ttcttcagtc
      961 atgggcggta aaactgaagg gacacaacgg gcaagcattg tttttcccga cccgggaggt
     1021 ccggacatta gtaaactatg cccgccggca gctgctactt ccagcgctcg cttcgctccc
     1081 tcctgtcctt taacctgttt aaaatcagct ccattttgta gcgggagctg tttactctgc
     1141 ggcagcggcg gaggcagttg actgccagca agtatcgcta aggcttcacg caaagatgta
     1201 gcaccaataa tatgtacgcc actaactaag gctgcttctc cagcattagc taaaggcaaa
     1261 aggatgttac gacagccagc gtggcgtgca gccatgacca tcggcagcac gccacgtacc
     1321 ccccgtagag tgccgtccaa cgccaactca cctagaaaag cccatggcac atcaggtaat
     1381 tgtagttggc cgtaagcctg taaaataccc attgccagcg ccaaatctag ttgcggtcct
     1441 tctttcttta agtctgctgg cgctaaattg acaataattc gctgttgtgg aaattgtaaa
     1501 gaactattct taattgcggc ccgcacgcga tcacgtgctt ctcgtacagc tgtatcgggt
     1561 aagccaacaa tatcaaagct tggcaattgg gatgaaaggt atacctccac atctaccatc
     1621 ctgccctcga tgcctacaat ggtgcagcca gtaactctgg ccagcatctt cattcctccc
     1681 ctaccatttt gtaccattat atagcaaaca tatgttcccg tcaaccaaac tttctcttaa
     1741 ctaggtgcaa tgatatatta aaatgaggtg agcagatgaa aaaaacacta ataatcatat
     1801 tattactggg tctgagcgcc ctcctcgggt tttactacct gcaacaaaca aaagatgagc
     1861 tggcagataa cactagactt ttttttgctg ccgcggtgat cggctcggaa caagcacaag
     1921 attatttagc accaaatata gaaaataaag aagcactgct aaccgccctg caaactgaag
     1981 ctttaattac aaaggctgaa attcgacagg tgcgcctgca ctcttacaag cgggcttcag
     2041 ttttagttga tttagagatc aacaaaaaag tgacttctct cgaagcacag tttatacgtg
     2101 aaggcaggac gtggcgtatt aactcactac cagcctttac tgtgttggag caagctttaa
     2161 tcagcaatct tgacgccgac caagtgactc tttttaccac gcagggtaaa catacctttg
     2221 ttatccaggc agatgatgct gtccaaagcg gcaacattgg cgatgccgtc gccattgagc
     2281 gcaatttgat ctattttaaa gcttacgcgg ctgaggaaac agctaaatta ttagcaatca
     2341 ctagtgatgc tttagaggtt gaaatgtctg gtctgcatcc tttagcagca gatgttgttt
     2401 attttcaacc tcaacaaaat agcttccggc tagttgacaa acaatccttg ttggtcggca
     2461 tggaagattt aactctttac tggcaagata atgaggtgaa agcagtgatg ctgccggaaa
     2521 actttctacc caccaacatt cgggttctgc taaatactac tggtttcact agtatctccc
     2581 accgccaggt taccctttct gcaaatactt cctatctgct cactgataaa gctgccaaac
     2641 agcaatatcg cttcacaagc gggcaaaagt taagcttcaa accgactgat aacggtattg
     2701 aggctacttt ccccgatagc cggaaacaaa gcttccccaa ccgtctgtat ctcatcccgg
     2761 atcaccacgg acagttacga gtagaaacac taaaacgtgg tactcctgcc tttaccccca
     2821 cctaccatgg acatctggaa ataaaagcag ctcaaggcgc tttgctgctt gttaatgagg
     2881 tgcctctgga aacttatctc tactctgtag tacccagcga gatgcccatt agttttggtg
     2941 ctgtgccgct gcaggtgcag gcagtagctg cccgcacgta tgctgtcgct gctatgtacc
     3001 ggggtgggtt taaacagtat ggtgcacatg tcgatgatag tgtctcctca caggtttata
     3061 acaacgtccc tgagtatgcg gctagtaccg cagcagtgaa tcatacccaa ggtgtgattg
     3121 taaaatatca ggacacaatt gccgatacac gtttcttctc tacttcagcc ggtgttaccg
     3181 caaacaagaa cgaggtatgg catgatgaag caagtggcag ctttcccggc acagccgttc
     3241 cctatttgcg tgctctttca caattgcgta caggacagct gcctgacgtg acaagtgaag
     3301 caggtgctcg tgaattcttt actcagagca atagggacag ctacgaccaa aattctccct
     3361 ggtttcgctg gcaggtggaa atgacggcag ctgagttgac agctgtctta aaacagtatt
     3421 tacctgaacg ttaccgggca cagccggact ttgtactaac caaagcagga gatgattttg
     3481 tcagtaaaga agtcgcggct gaaccgctgg gtaagctgac agatttacga gtcatccgcc
     3541 gcggggctgg aggaaatatt ctggaactgg agctagttgg tgaaaaaggc acttaccgcc
     3601 tgctaaaaga atacacaatc cgttttacac tgcgaccaat aagcatgggt agcggcaaag
     3661 atgtcatcct cacccgtcac gatggttcaa cacttgccaa ttacactatc ttgcccagta
     3721 cctttatggt ttttgaagtt aaacgtgata gtagcaataa tattacctcc atcctctttc
     3781 aaggtggcgg taatggtcac ggcgtgggcc taagccaatg gggaagccgc ggtttagcgg
     3841 aagacggccg cagctttagc gagatcctac agcattacta tcccggctgt actttgacaa
     3901 acatctacta gtgttatgaa tattgttttg ttttaataat ttgttcagat atattaagtg
     3961 aaattgctgt atgctaaaac caacgaagaa gttaccttct ttaccccttt tatgcgagtt
     4021 gctcacggac acgtggcagc tctttttctt tttatttgct tttacgccaa gggcaaacta
     4081 taataataaa aagataattt ttaccagggg ggaaccagaa tgcttaaaca aattcaagat
     4141 gacatgaaaa cagcgctgaa agctggtgaa aaaaagaaag tttccacact gcgcatgtta
     4201 attgccgaca ttaaaaatga aagcatcaat aaacgttcag ctttgtccac tgacgaaata
     4261 ctatccgtta tccaaagaga aattaaacaa cgtcgcaatg ctaatgagga atttaagaaa
     4321 ggcaaccgtc ttgatttagt tgcagaaaat gaaatagagg tggcgatctt aaccgcatat
     4381 ctgccacagc agttaggcga tgaggaatta gaaacaatag tgctgcaagc agtcagtgca
     4441 gtaagcgcca cctccctcaa agagatgggt aaggtgatga gcaaagtaat gccgcaagta
     4501 aaaggaaaag cagatggtag acgtgtacaa gctgcggtaa aaaaggtctt aagctaaata
     4561 agtatgtaaa aacggaggca tcgctgcctc cgttttttta gtatttgaaa aatacatgtg
     4621 aaccaatgct tgtagttacc gggtgactac gcacccaggc attacttgtt ttggctgggt
     4681 tgtaaaaccc cgttgcccca gcagttggat ccaaaccttt aagtgcttcg cgcacagctc
     4741 gttttgcact ttcgctggcc ggttgattaa tgcgtccgtc cagcaccggg ctgtattgat
     4801 aacgtccact ttcaacttgg tagacaacac cactaatagt attgggataa cgttcgtttt
     4861 ccacgcggtt gagaatggtc gcagccactg ctacctgacc atcaaaaggt tctcctgctg
     4921 actcagcatg taccaagcgt gcaaacaatt ccagatcagc ttccgtaaaa ttatggttat
     4981 ttacggccac caaagtttgt gtagtttcta cttttgctgg cgctgccata cttttcgctt
     5041 ttactgttgc tgccggcttt tctgacatca tctcttgcat ccgtgtaagg gttttttcag
     5101 ctaacactcc gtcggttttt aacttttgct ctttttgaaa tgctttcaga ctgccataag
     5161 tcatcgggcc aaaaacacca tcaagatgac cttcacgata tccatgttga ttcagaaact
     5221 cttgcataac ggctacatct tcaccacgat cgccaatttg taattctctt gcaccgaatg
     5281 ccagcgtttg ggctgctgca ccagttatca gtgtcatcaa gaaacatgta gtaatcatta
     5341 ttgttagtaa ttttttattc atctttttta cctcctctgt gtaaccgcgg agtgtactga
     5401 tcaatgataa catgttccag caaatgaggc aaaaagcgag agcttgccct atctggaact
     5461 atcccagctc atgagagggc aagccaaagg caaaatagga gatggactaa ttcaccataa
     5521 tccgtcatta tcctcattat tggtatattt ggggaacagg ccttccaccg tgaagaaccc
     5581 ttacctgtca ggaagaatcc ccttaaaagt cgtcttgtta agaaaatggc atgaaaaagc
     5641 tggcataaca ttcctgccac tcaaaggaac tgaacatccg cttgtcgttc tattgtgctt
     5701 tttctaagcg gttagtggaa ataaaaaata ggtccagtaa gttggaccta cgtttaaaag
     5761 gcatttttga tcagcgtaac ttgttcgctg acaccgtcta cggtgacaac atcaaaacga
     5821 taagccggag ctgtgttacc tgtaagatga atatattgca gtgctaaatg acgaattttt
     5881 tcctgcttgc gggcatcgac tgcttcggca ggggtaccgt gtcgctggga gcggcgcgtt
     5941 tttacttcga taaaaacgag cagctggcca tctctagcta cgagatccag ctcacctcgc
     6001 tcacaccgcc agttttgtac caatacttga taaccgtgtt tagtgagata agcagctgct
     6061 agttcttccc cccgtcgacc caaagccttc acctttcttc ctcggctggc aaaagcataa
     6121 aagattggcg gtggaaagga gtagccccca gctgagcaat ggctttccgg tgttcacgtg
     6181 taggatagcc cttatgttca gcaaatccgt atcctggcag cagttcagct agctcgcaca
     6241 ttaagcgatc cctcgttact ttagcaatga tggaagcggc ggcaatcgag gcagaaaggc
     6301 catcaccgcc aataatccct tgctgcggat aaggcaaatc ttctaactcc agagcatcca
     6361 gcaagacata atccggttta gttgtcaggc caagtatggc acggcgcatc gccattttag
     6421 tagcatttag tatattatac tggtcaattt ctgctacttg ccccgcccca actgaccagg
     6481 cgatggcgtt tttcgtgatg atcgtatata gtttttcccg gttaagaggg gatacttttt
     6541 tggaatcatt taaaccagtc acagaaaaat tctccggtaa aattacagca gccgctacta
     6601 ccgggccggc caaaggacca cgcccagctt catcaacacc ggcaatatat gtatagcctg
     6661 ctaaacgagc cttcctttca aacttgtgta aggcagacaa gcgttctttt tctaaacgct
     6721 gggcttccag ctgatgtaaa tagctttgca ccacatgacg tacccctact cgctgatcag
     6781 ctgataattg agctagttca gcaggcgtag gtactgtttc tttaaagtag cggcgaatct
     6841 gcgccactgt cattttcgtg aaattgctca ttattttctc ctaattctct cacctgctcc
     6901 aaggtcaggc gaccaagacg tgcttcacgg aaatctttga ttaaaagccg ggcggcctgt
     6961 tctaaatcaa cctcgccgcc ttttactaaa caaccacgcc ttttgcctat ttcctccagc
     7021 agcaggcgtg tctccccgct aacactacca agtttatagc gctgctgcaa attctgtggt
     7081 acgttggcga gcaggtaagt catcaattca agtgccaatt ctacagggtc atatatctca
     7141 tccttgattg cccccacagc cgctaaacta aaagcttgcc gtttactagt aattttaggc
     7201 cacagtagac cgggagtatc aaggagctct aaatttgctg ctaaacgcag ccactgcttc
     7261 cccttggtaa caccaggctt gtcgcccgtc atagcggcac tacgcccgat caggcggttt
     7321 attaaggacg atttgcccac attagggata cccacaacca tcaaacgcaa aggacgctgc
     7381 gctcgtcgtt gggggattgt tttcaaaatc gctagcagat tttttacacc ttgcccacta
     7441 ttggcattaa gcgctgctac ggcaaagtaa ttttcacgta atgcttgtac ccatgcagaa
     7501 gtaacttgag ggtttgccaa gtcagcacga gttaaagcag ctaccacagg ccgtcttgct
     7561 aaaaggtcgc gaatatcggg gttacggcta gagtaaggaa tgcgggagtc aagcacctca
     7621 attaccgcat caactaattt taagttttct cttaacagac gtttggcctt ggccatttgt
     7681 ccgggatacc attgtatttt catgcaatca tcctctgctt ttaggggtgg gagattttgc
     7741 gtatgttatc caacggccaa aaaataaaca ttgctttacc ctttatttcc gccatttcta
     7801 cgaaacttac agcaggatgg cgactatcca tactattatt acgattatct cccatcacaa
     7861 aaaatgttcc ctccggtacg gttgccggac cataaggttc ccgggcttga tcagcaatgt
     7921 atgtttctgt tattttttct ccattgacaa aaactccatc cggattaatc actacttcgt
     7981 caccagccgt agcgataatt cgtttaataa agtcacggtc ttcagtagcc tgaaagacaa
     8041 caatttcacc tggctgcggt tctcgatagt aatattggat tttactgacc aggatacgct
     8101 cacggttatg cagcgtaggg gacatagaat ccccttttac taagaagacc tctagtaaaa
     8161 aggctctaat aactagcgcc aagacaattg ctagtaaaat cgatttaatc cattcccaca
     8221 tctcattctt atccgcttct tgcatttttt tccttctttc ttacacaaga gatctatgat
     8281 aacaacttaa tattaactta tgctagtgag agcatctata ttgcttcaaa aaatgattac
     8341 tataaacagg cttcactaac tgtgaagctg acaagcttat taactagcaa gtattccaat
     8401 attaattaca attgcaaaaa aggactgttt agacagtcct cttttttagt aacgtctttc
     8461 tttgatgcgg gctgccttac cagtaagttt ccgcaaatag tatagcttag cacgacgcac
     8521 aataccgtga cgtacaactt caattttttc aagtttcggg gaatgcagag ggaaagtccg
     8581 ctctacaccc acattataac taatacggcg cacggtgaag gtttcccgca gaccgctgcc
     8641 ctgtctttta atgaccacac cctcgaaaat ctgagtacgt tctcgggttc cctccacgat
     8701 tttcacatgc acacgtactg tatcaccagg tgaaattgac ggtagatctt ttttcatctg
     8761 ttccgcttca atctgacgaa tcacatccat gtaattgccc tcctttcatc acaatgatgg
     8821 ctataaagaa gcttgcgcct ttcagtttcc taaaacaagc tttgatactg ctgacatcag
     8881 cagtttactt tcagcaaagc aaaatatttt cctagcagtg caagaaaaat tataccatac
     8941 aaataattgg tttacaacaa atcatctagt tgacgacgct tggcttctgc agcaagaaac
     9001 tgcttttctt ctgcagttaa ttctgccttt gacaataaat cggggcgaga gattaatgtg
     9061 cgcagcaacg cttgtttacg tcgccactgc ttaattcttt catgatttcc agacagtaaa
     9121 atttcaggca ccgctaaccc ctgatattca gctggtcgag tatattgggg atattccagt
     9181 agattatctg taaatgattc ttcagcagtt gaggcagcgg gtaaaactcc gggcaaaaga
     9241 cggaccacag catctgcgat ggccatggct ggtatttcac caccggtaag gacaaaatca
     9301 cccagtgaga tttcttcatc cactagtgca tccttaactc tttcatcaat cccctcgtaa
     9361 tgcccgcaaa gcagaaagag tcgttcttct tgtgctaact gccgcgcttt ttgctggctg
     9421 aagggttgac cctgcggcga catcaaaatg acgcgtccgc gctgcttgga attttgacgt
     9481 aaatgctcta gtgccagaaa aaagggttcc gcttgcatca ccatgccggg gccgccgcca
     9541 taggggtagt cgtcgacgtt acggtgcttg ttctgcgcaa aatcacgtat atcagtaatc
     9601 acgattttta cctgtccgtc ctcggcggca cgcttaataa tactttctgt aagtgaagca
     9661 aacatgttgg ggaaaagggt taagatgtca atacgcatct tttcacctca catcagcccc
     9721 tcaggcaaat ccacttccag acgtgcatgc tgcaaatcaa tctttttaac aacagttttt
     9781 agtgccggta ttaagatttc aggaccttca gattgacgaa ccacatagac atcattactg
     9841 cctgtctgca agacatcact aacctgacca agttccagtc cggccagcgt aaaaactgtc
     9901 agcccgataa tctggtctag atagtaagaa cctgcaggca gctcgacccg ttctgttaaa
     9961 gggatcttca gcagtgcacc tttaaagcgc tctgcgctgt ccatatcctc gacatcctgt
    10021 aaaaacacaa cccaaaagcg tccatggaca aaagcgtctt ccacttgata agcgctgact
    10081 ctttccccat gctccataaa gacgcgtttt aaagatttta cgcgttccag aaagtcggaa
    10141 aggggcagga ttttaagacc gccttttacg ccatgggtat taacaactct gccaatggtt
    10201 gccatctgct ccacaaaatc accgcctact ggacaatttc cacaaagaca cgttttcttt
    10261 ctgtagtagc agctgcgtta accacagtac gaatagcttt agctatacga ccttgcttac
    10321 caatgacttt acccatatcg tctgcagcta catgcagttc aagcgtaatc gctcgctcat
    10381 cctcaagctg ctctactcgc acttcgtcgg ggttgtccac taaagctttg gcgatgaatt
    10441 caaccaaatc cttcatgttc atcaccgtcc atttcataaa ctatttaccg ataccagctt
    10501 ttgttaacag tgatttcact gtatcggaag gttgagcacc tttttgcagc catgtttgtg
    10561 ctttctcgtc gtcaatcttc aatgtaactg gttcagtggt agggttgtaa acaccaattt
    10621 catcaataaa tttaccatca cgcggcgaac gggaatccgc aaccacgatg cgataaaaag
    10681 gacgtttttt tgcaccaacc ctttttaaac gaattcttac ggccatacat ttttcacctc
    10741 ctccatagag taatcaacta ttacataaag ggaaattttc ctttactttt accctttagc
    10801 ttacctttat caagcttact aaactgtttc atcatctttt gcatcgtatt aaactgcttg
    10861 agcagactgt taacgtcttg tacagtggtg ccgctgccag cggcaatacg cttccgacgg
    10921 ctgctgttaa tgatggtggg gtttaagcgt tcttttttcg tcatggattt gataatcgct
    10981 tcggtacgag caaattgctt ttcatcgaca tccaggcctt taagcttctt gttcatacct
    11041 cccatacctg gcaccatttc cataatctga ttaagcggtc ccatcttttt caactgctgc
    11101 atttggtcca agaaatcttc tagcgtaaac tgctgagagc ggattttctg ttccagctcc
    11161 cgcgctttgt cctcatcaaa agaagcctga gctttttcaa taagtgacaa gacatcgccc
    11221 atgcctagaa tacgtgaggc catccggtcc gggtgaaaag tttcaaaagc atcaatcttt
    11281 tcacccgtcg ccgcaaactt aattggtgca ccggtcacag cacgcaccga caaggctgct
    11341 ccgccacgcg tatcaccatc tagtttggtt aaaacgacgc ccgtcaaacc tagtcgctct
    11401 ttaaaagact gagcaacatt gacggcatcc tgtcccgtca tagcatcaac caccagtaaa
    11461 atctcttgtg gctgtgcagc ttcttccata cttaccagct cattcattaa ttcttcatcg
    11521 acatgcaaac gtccagctgt atccacaatg acataatcat ggcgtgattt ttttgcatgc
    11581 tccagggcag ctttggccgt ttgcaccgga tcctgttgac ccatttgaaa caccggtaca
    11641 gccagttgtt cccctaatac ctgcaattgt ttgatcgcag ccgggcgata aatatcagcc
    11701 gctaccaaaa gcggcgtgtg attcttcttt tttaacaagt tggccagttt agccgcagag
    11761 gtagttttac cagcaccctg taagccaacc atcatgatca ctgtgggcgg ttggttagct
    11821 tgattcagtt tagctgccgt ctcccccatc agctttgtca tttcttcatt aacaattttt
    11881 atcaccattt gaccaggtgt caggctggct aagacatcac tacctaaaga acgctctttg
    11941 acggtattga caaaatcttt tactacttta aagttaacat cagcttccag caaagccaaa
    12001 cgaacctcac gtaaagcagc tgtaacgtct ttctctgtca gtttgccttt tccccgtaga
    12061 cgtttgaaag tatcttgtag tttgttggac aggttttcaa aaatcatgct ttgtttcctc
    12121 caaacaatta atccaatagc ttctccacaa tggtcagcgc ttctctgcgg ttttgctctg
    12181 gcatctcact cttagtaagt atttcgcgta agcgttgtag ttctttttcc tgcgccgtaa
    12241 agcgaaccag caagccaagg cgcttttccg cattttccag ggagcgtacg gcccgcttca
    12301 ccagatcatg caccccttga cgggaaatgc cttcattatc ggctatttca gccagactca
    12361 aatcatcttg ataataaagc tctaggtagg cgcgctgttt ttttgtcagc aggttaccat
    12421 agaagtcgta taataaattt acccgtgttg tatcctcaag cattgtatca catcctaccc
    12481 atgtctgtaa agcaaagata ctttacaggt agattataac ggatttcttc tgccaaggca
    12541 agttcgacat tatagtaagc ttaattaggt taaccttgac cacaagcaaa gcgcgtcaga
    12601 tctgtgaact ccgttaatct tacctggtct tccagcacat agatacggtt tgatactttt
    12661 acagcactag ctagatcatg ggtaaccatc aacagcgaaa atcctttagc attttgcagt
    12721 cctttcaaca acctcaataa atttgcttta ctggaggcat ctaacatcga agtgggctca
    12781 tccgcaatta ataatttggg cgccatcact agtgctcggg caagagctaa acgctgcttt
    12841 tggcccccgg aaagctgcct gacttttttc gctaaaaatt cagcggtctg aggtaagcca
    12901 acatcaagta aagcactttt taccgccgcc gataaatcca tttttgtatg gtttaaaagt
    12961 aagggctctg ctaccgcact ttgcacagta aagttggggt taagagcagc ctgcggatct
    13021 tgaaaaacca tctgcaggcc gccttgctga cgatgcagct gtttaaagtt cgctttttca
    13081 ccggcaaaat atacttcccc cgcctcactg gctagaaagc cggctaaaat ttgtgccaaa
    13141 gtgcttttac caataccaga ttctcctatc agggagacca cttcgcccgc agcaacttct
    13201 aggtttaaat c
//
LOCUS       DASRMS010000015         1158 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_338285, whole genome shotgun sequence.
ACCESSION   DASRMS010000015 DASRMS010000000
VERSION     DASRMS010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_338285"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1158)
                     /locus_tag="VFC74_00965"
     CDS             complement(<1..>1158)
                     /locus_tag="VFC74_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23941.1"
                     /translation="ITPIITRLKEFVAGFRDLSPEAKKMIVVVAGVTAAIGPFLLILG
                     KVIAIAPAVGAAVTAMTGPIGVIVMAIAAAVAAIIYYWDDIKAYFTEGAGASTFTSIS
                     EAATKVKDVLIKVFTTIKDVVTKIWDKFGGYITGAFSAVFDTIMAIVDWFVEQVGNVA
                     DFFSAIVDLDFSGALDALGKIFTSTFNGILRIVTNIIGQAANIVAGFFDAIGMKTWAK
                     ATKAFADDMVAAYVPLEKKKKDDVDVTEKQEKAQLKVAKAVERTAVAVASVGRESNIE
                     PIVGAGITMLEVEPVVVPPIDYDPYAQSLEEMNAITLDYGSIIAGGLTDFATSIGEAF
                     AEGNFEGLGDGLISAMGSMMSQFGSMLIASAIASEKLQTLITNPWTALAAGI"
BASE COUNT          322 a          305 c          197 g          334 t
ORIGIN      
        1 tataccagcc gccaatgctg tccacggatt tgttattaag gtctgtaatt tctcagatgc
       61 aatagccgaa gcaatcaaca tacttccgaa ctggctcatc atactaccca ttgcgctaat
      121 taaaccatcg cctaatccct cgaaatttcc ttctgcaaat gcctcgccta ttgatgttgc
      181 gaaatcagtt aaccctcccg caattatgct tccgtaatct aaggttatag cgttcatctc
      241 ttctaaactc tgagcgtacg ggtcataatc aataggcggc accacaacgg gctcaacttc
      301 cagcatcgta attccagcac ctacaattgg ctctatattg ctttctctac ctacagatgc
      361 aactgcaact gctgtgcgct ctactgcttt ggcaactttt aactgcgcct tttcttgctt
      421 ttccgttaca tcaacatcat cttttttctt tttctccaac ggaacataag ccgccaccat
      481 atcatctgca aaggctttag tcgcttttgc ccacgtcttc attccaatag catcgaaaaa
      541 cccagccaca atgttagcgg cttgaccaat gatatttgta acaatgcgta aaatgccgtt
      601 aaaggtgctt gtgaaaattt tacctaaagc atccaacgct ccagaaaagt caaggtcaac
      661 tattgcactg aaaaaatccg ctacattacc gacttgttca acaaaccaat ccacaatggc
      721 cataatcgta tcgaataccg ctgagaatgc ccctgtaata taccctccga acttatccca
      781 gatctttgtt acgacgtctt taattgtcgt gaagaccttg atcaaaacgt ccttaacctt
      841 tgtagctgct tcggaaattg atgtgaatgt ggaagctcct gcaccctctg taaaatatgc
      901 ctttatatca tcccagtagt agataatagc tgcgacggct gcggctattg ccataactat
      961 aacgccaatt ggcccagtca tagctgtgac cgctgcaccc acagctggcg caattgcaat
     1021 gactttcccc aggattaaaa gaaaggggcc aattgcagct gtaactcctg caacaacaac
     1081 aatcattttt tttgcttcgg gtgataaatc tctaaatcct gcaacaaact ctttcagacg
     1141 tgtaattatt ggagtgat
//
LOCUS       DASRMS010000016         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_807932, whole genome shotgun sequence.
ACCESSION   DASRMS010000016 DASRMS010000000
VERSION     DASRMS010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_807932"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..694
                     /locus_tag="VFC74_00970"
     CDS             <1..694
                     /locus_tag="VFC74_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23942.1"
                     /translation="DASRIWGSIVTFFSSTWQTIRSNAALGWSNVKLGITSAWNDLKA
                     DASRIWGSVKGIFSNTWTNIKNTAVNAWNGLKISAANIWGGIKNTISNQISNIKKLFN
                     FKWSLPKIKLPHFSVSWSTAGFWGKVGDFLGLPGKPNIGVNWYAKGGIFNQPSVIGVG
                     EAGREAVLPLDSNTGWADTVADRLASAMQGVQLAGAGAGGGDIYVYIGNEQVDAYVYR
                     SQDRRNIRSNGR"
     gene            700..>1058
                     /locus_tag="VFC74_00975"
     CDS             700..>1058
                     /locus_tag="VFC74_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23943.1"
                     /translation="MALITIDSTPLPDPVEYSVSLQDIDSENTRRTEAGVLTRDRVRA
                     GVYKIQVTWKVTKTQLKTITDSIGPAQFSVTFFDPTTSTNPTKNMYSGDRTGKLMLFK
                     DPNTPSESLWELSTSLI"
BASE COUNT          306 a          231 c          282 g          239 t
ORIGIN      
        1 tgatgcctcc cgtatatggg gctcaatagt gacctttttt agtagtacat ggcagacgat
       61 tagaagcaat gcagcgctag gttggagcaa tgtaaagctt ggcattacca gcgcatggaa
      121 tgacttaaaa gctgatgcct caaggatatg ggggtctgtt aagggcatct tctctaacac
      181 atggacaaac ataaaaaaca ctgcagtgaa cgcctggaac gggcttaaaa tcagcgcggc
      241 taatatttgg ggtggtatta aaaacactat tagtaaccag attagcaaca ttaaaaagct
      301 atttaacttc aagtggagcc taccgaaaat caagctccct cacttttccg tgagctggag
      361 cactgcagga ttctggggta aggtaggaga ctttttaggt ttgcccggca agccaaatat
      421 cggtgttaac tggtacgcaa agggcggcat cttcaaccaa ccctcggtga tcggcgttgg
      481 tgaagcaggc cgggaagcgg tactgccgct agatagtaac actggttggg ctgacacagt
      541 cgctgatagg cttgcatcag ccatgcaggg tgtgcaactg gccggcgccg gagcaggtgg
      601 cggtgatatt tacgtctata tcggcaacga gcaggtggat gcctacgttt accgcagcca
      661 ggaccgcaga aacataagga gtaacgggag gtaaagtaga tggcgctaat tacaattgat
      721 tctacacctc tacccgatcc tgttgagtat tctgtcagtc tgcaggatat agacagcgag
      781 aacaccagac ggacagaagc gggtgtctta accagagatc gggtcagggc tggtgtatac
      841 aaaatccagg ttacctggaa ggttacgaaa acgcagctaa aaacgattac agactctata
      901 ggccctgcac agttcagcgt gacttttttc gacccaacca caagcacgaa tccgactaaa
      961 aatatgtact ccggagatag gacggggaaa ttgatgctgt ttaaagaccc gaacactcca
     1021 agcgagagcc tgtgggaact gtcaacatca ttgattga
//
LOCUS       DASRMS010000017         1942 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_70077, whole genome shotgun sequence.
ACCESSION   DASRMS010000017 DASRMS010000000
VERSION     DASRMS010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70077"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1668
                     /locus_tag="VFC74_00980"
     CDS             217..1668
                     /locus_tag="VFC74_00980"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK23944.1"
                     /translation="MTQLTEAQDTATDLSGDPVRWRILAVLLMAIFMSLMSVSVVNVA
                     LASIQDALGASLSDIQWVLSGYALTFGVVLVSAGRAGDLMGRGGIFILGTVLFTLASV
                     AAGLAPDAQWLNAARFVQGVGSGFMNPQAVGMIQQYFRGSERGRAFGFFGTTVGVSVA
                     IGPVIGGLLIQLGGLEIGWRLTFLINVPVGLLIIVLALRWFPRPLIHKPKLLEHKGLS
                     SLDPVGALLLGLGVLAVLYPFVEADPSGLIWSLLPAGLLLFYLWARWERWYTQRGYSP
                     MVDLKIFATSSYRNGSIIMTLYFLGMTSVWVLIPLYVQQGLGFSAFEAGMVGIPSALL
                     SAYSANWAGKRVNRYGRKVVIGGLLFAIFGLLASVGVVFLHEYLDLSIWWLLLSLAFF
                     GLGQGSVVSPNQALTLAEVPLAYAGSSGAIMQTGQRIGTSVGIAVITAIVFAVRPYSS
                     WSVAVSVGLLAVTLVILLALAMAIKDLRDRRVV"
BASE COUNT          371 a          378 c          552 g          641 t
ORIGIN      
        1 caaggccact aattgaggtt tattatttgg cttatttgcg gcaattaatt ccacgtagcc
       61 gctacgcggg ctgtgcgtta tttatatatt tctagtcatg gggcagcaag tgaatactta
      121 agggcctatg tatatccgcc aaattaacgt taatgctgtg cacccctagc agttcattta
      181 cactagcgac gctactttta tttttacggg ggttttatga cgcagctgac cgaagcacaa
      241 gataccgcaa ctgatctcag cggtgaccct gtgcgctggc ggattcttgc tgtgttgctg
      301 atggcgatct ttatgtcgct aatgagtgtc agtgtggtca atgtggctct ggcctcgatc
      361 caggatgcgc tgggagcttc tctgtcggat attcagtggg tgttgtctgg ctatgcgctg
      421 acctttggtg tggtgctggt cagtgctggg cgcgcagggg acttgatggg ccgtggtggg
      481 atttttattc taggtacggt cctttttacc ctagcttcag ttgctgcagg tttagcccca
      541 gatgcgcaat ggctcaatgc tgcgcgtttt gtgcaaggtg ttgggtctgg gtttatgaac
      601 cctcaggccg ttggcatgat tcagcagtac tttcgcggct cagagcgtgg acgcgcgttt
      661 ggtttttttg gtactacagt tggggtttct gttgctattg gcccggtgat tggcggactg
      721 ttaattcagt taggtggctt ggaaattggt tggcgactga cttttttgat taacgtgcct
      781 gttggtctgt taattatcgt attggcactg cgctggtttc cacggccttt gatacataag
      841 cccaagctgt tggagcataa aggcttaagt tctttggatc ccgtcggagc attattactt
      901 gggctgggcg tgctagcggt gctttacccc tttgttgagg cggatccgtc tgggcttatt
      961 tggtcgctat taccggcagg gctgctcttg ttttatcttt gggcacgttg ggagcgttgg
     1021 tatacgcaac gtggctatag cccgatggtg gacttaaaaa tcttcgccac ttcaagctat
     1081 cgcaacggta gcattattat gaccctgtac tttttaggca tgaccagtgt ttgggtgttg
     1141 attccactgt atgtgcagca agggctcggg ttctctgctt ttgaagccgg tatggtcggc
     1201 attccttcgg cactgctctc tgcttattct gctaactggg caggcaaacg ggttaatcgc
     1261 tacggtcgta aggttgtaat aggtggtttg ctgtttgcta tttttggctt actggccagt
     1321 gtcggcgtcg tgttcttaca tgaatatctg gatttaagta tttggtggtt attgttgtcc
     1381 ctagcgtttt ttggcttagg tcaaggttca gtggtcagcc ccaatcaagc gctaactttg
     1441 gcggaggtac cattggctta tgcaggcagt tcaggtgcca ttatgcaaac agggcagcgc
     1501 attggtactt cggtgggtat tgcggtgatc acagccattg tctttgctgt acgcccgtat
     1561 agctcgtggt cggttgcggt cagtgttggc ttattggcag ttactttggt gattttatta
     1621 gcgctggcga tggcgattaa agacttgcgt gatcggcgtg tggtttaggc ggcaagcagg
     1681 gtgtgtatga tatggatgcc gtaaatgtgc ttgcttgact tgatttttat cgaaaacttg
     1741 tgatgaagtt tgctttaaat acaagattta ggtagaaggg gttagcaacc attaattgtt
     1801 aaccccgttt agcattttac ttattggctg tcaggagtgg caactgtctg tcctgtaaga
     1861 atttcttgtt tgcgcttagc ttctggcaaa tcattaatac agtagttgat atagtagaga
     1921 gtgaaaatga atgtataaaa ag
//
LOCUS       DASRMS010000018        10315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_3272, whole genome shotgun sequence.
ACCESSION   DASRMS010000018 DASRMS010000000
VERSION     DASRMS010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 10315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10315
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_3272"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            246..1088
                     /locus_tag="VFC74_00985"
     CDS             246..1088
                     /locus_tag="VFC74_00985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK23945.1"
                     /translation="MKKIALVTDSTADLPPSLALEWDVHVIPLKICFAADEFYDGELA
                     AEDFYRRLQSAKELPFSSQPAPEDFLKLYQSLLNKYDEVISIHLSSNLSGTVNAAWVA
                     AETLQGKVHVIDSKTISLGIALMVNEAVRCIRDGFDAFQIKERMDTARKNIETMFTLD
                     TLEFLQKGGRIGKVTGLVGSLLNIKPVIRVNEEGIYIPAGKTRSQDKSLQQIVQGLQQ
                     LANGRKIKALAVAHGAALEAAQRLKDALEKTFNIPACMFTQVSTVIGVHTGPGTVGTA
                     VHFE"
     gene            1348..1791
                     /locus_tag="VFC74_00990"
     CDS             1348..1791
                     /locus_tag="VFC74_00990"
                     /inference="COORDINATES: protein motif:HMM:NF013440.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HZK23946.1"
                     /translation="MQTAISKVRLDHLEKHIAVIKDERKQTLEQYFTKSINERNDFND
                     FLDNYLQHIEKFVKSANNAQALNLQEAPFIFIGCDVEVEDLDTADIINYHLTDPIQER
                     VGEDDVSYLSPVGKALLLKKVGDEIQVKTPGGTIRYKIRAIHFKA"
     gene            complement(1860..3305)
                     /locus_tag="VFC74_00995"
     CDS             complement(1860..3305)
                     /locus_tag="VFC74_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK23947.1"
                     /translation="MNSLIVLKTLGILLLCESVAMLPSVAVAIIYKETAISAFIITIL
                     IAAVVGFLLYLIKPKEKMIRYKEGFAIVSFGWIIASIIGALPFLFSGALASPVDAFFE
                     TVSGFTTTGATVIPNVEGLPNCLLFWRSFTHWLGGMGILVLTLALSPALGIGTFQILK
                     AESPGPISSKLSTKVSDTAKILYITYLAITVLMILFLWLGGMPLFDTLCHTFGALGTG
                     GFSIKNSSIGAYNNVYFEVVITTFMILSGVNFSLYYLAFKKRSLKELWQDTELKVYLG
                     IIAAYILVIAFNIYGPNFNSFAQSLRHASFQVGSIITTTGYATTDFDLWPDLSRTLLL
                     TLMFIGACAGSTGGGMKVIRIHLIFKYIQREIGTLIHPRAIKAVKVNDETIPETVLSG
                     VMAFSLLYFLIFIVATLLLLTQNLDMISAVSAAACTLGNIGPGLSAFGPTKTFAPLSD
                     FGKNILSICMLFGRLELYTMISLVFPSFWRK"
     gene            complement(3256..4665)
                     /gene="trkA"
                     /locus_tag="VFC74_01000"
     CDS             complement(3256..4665)
                     /gene="trkA"
                     /locus_tag="VFC74_01000"
                     /inference="COORDINATES: protein motif:HMM:NF007041.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HZK23948.1"
                     /translation="MKVIIIGAGKVGYQLVESLVNENHDVTVVDNNQNVIDKLNDNFD
                     VLAMKGNGVSSRLLEKLDCSSADLLIAVTNSDEANIVSCITAKRLGTNSVIARVRNPE
                     YVAELDFMQKNLDIECIINPEYATAQEILRLLLNSHTSYAVDLAKGRVRISEVPINAT
                     ANLSNKQIKDIDLPESVIITAIARNGNVIIPNGFDYIYPQDTIYIMGERAAVDAFARS
                     MGVLIDNSKVRNVLIIGGGKTAFYLARKLEQLGINVKIIEENLHRCRELAEGLDNTLV
                     LHGDGTDIALLKAENIDEMDAFVSLTGYDEENLLVSMLAKQLGAKKVIAKVSRSSYDS
                     VVETIGLDSAVSPKLITARDILRIIRGGKILSMSLLIGGRAEVFEIIPQEGTPIVNKP
                     LKDIGIPKGVIIGAIFRNEQIIIPNGDSVIKSTDRVIVFTLESLIEKVKKLFNTNGGG
                     VPINELINSAKNSGDIASL"
     gene            5069..6646
                     /gene="serA"
                     /locus_tag="VFC74_01005"
     CDS             5069..6646
                     /gene="serA"
                     /locus_tag="VFC74_01005"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HZK23949.1"
                     /translation="MKVLVSDSISELGVAKLQEKIQVDVKTKLTEEELISIIPEYDAL
                     IVRSATKVTRPIIEAGKKLQVIGRAGVGVDNIDIEAATERGVIVINAPEGNTISAAEH
                     TIALMTALARNIPAASRSLKAGEWKRSTFMGVELYKKTLGVIGIGRIGSEVIKRAQAM
                     GMNILAYDPFISVERAEKLGITLTSPEDIYRQADFITLHTPKNNTTFHMIGEEQIAMM
                     KDGVRIINCARGGLIDENALCAALQSGKIAGAALDVFEEEPAVANPLCALPNIIVTPH
                     LGASTEEAQINVAVQVAEQIVSILAGEAPLTAINVPSIPPETMSEVKPFIPLMATLGS
                     FYAQVFGGQVEAIDLFYNGEIANFTVAPLTTSFLIGFLSTFLEDTVNAVNAPVLAKQR
                     GIKVREVISTSVENFTSLVTVNVKQGAEEHTLAGTLFNNNDMRIVQIDKYRIEVIPSR
                     FMLVTNHLDVPGVVGNCGVILGEENINIAGMQVGRQSIGGEAVMVLQVDSTVPESTIA
                     KVQNLEGMFSVKFVQLN"
     gene            complement(6701..10225)
                     /gene="nifJ"
                     /locus_tag="VFC74_01010"
     CDS             complement(6701..10225)
                     /gene="nifJ"
                     /locus_tag="VFC74_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003511654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HZK23950.1"
                     /translation="MAKIETMDGNQAAAHASYAFTEVAAIYPITPSTPMAEGVDEWSA
                     HGKMNIFGQPVKLVEMQSEAGAAAAMRGALQSGSLSSTYTASQGLLLMIPSLYKMVGE
                     LLPGVLHVAARSIATHALSIFGDHQDVMACRQTGVALLASSNVQEVMDLACLAHLAAI
                     KARLPFIHFFDGFRTSHEIQKIQVLEYEDIKALVDQEALAAFRQRALNPEHPVVRGTA
                     QNPDIYFQQREGQNPFFAAVPNIVQNYMDQFGEMTGRHYQLFDYYGALDAERVIVAMG
                     SVCDTISETIDFLLARGEKVGVVKVRLYRPFSVEHFLQALPATVKKIAVLDRTKEPGA
                     PGEPLYLDVVKALQNAAQKPVIVGGRYGLSSKDTRPSQIMAAFNNLNQPQPKDNFTIG
                     IIDDVSHTSLAEEEIVNTTPAGTISCKFWGLGSDGTVGANKSAIKIIGDHTDLHVQAY
                     FSYDSKKSGGTTVSHLRFGQKPVQAPYLVFDADYIACHNPAFLHHYDLVKGLKKGGSF
                     VLNCAWQAEEVAAQLPAALKQYLAKNEISFYIIDALAIANQVGLGNRINMVMQAVFFK
                     LAEVIPVEEALRLLKDSIVKMYGRKGENIVKMNHAAVDKALEALIKVDVPQEWQTAAD
                     EKLPVKDEPDFVKNIQRPMARQEGDELPVSAFTGMEDGTFPPGTTAYEKRGIAPQLPE
                     WQIDKCIQCGQCTYVCPHATIRGYLLDEEEAERAPETFQTIKAVGKGIGHLQYRVQVS
                     PLDCTGCGNCADICPAPDKALIMQPAAQVTEKEADNWEFAMTVTTKDDLLNPHTVKGN
                     QFVRPLMEFSGACPGCGETPYIRLLTQLFGDRMMISNATGCSSIWGASAPSIAYTTNA
                     EGKGPTWVNPLFEDAAEFGFGMLVGVLQKREKLAALIEEALQTELPPQLQEALTQWLK
                     DKDNGEAAKAATAKVRAALDQVNSPENSLLLEIKGRQDFLTKRSVWSIGGDGWAYDIG
                     YGGLDHVLASGEDINIFVMDTELYSNTGGQSSKATPTGSVAKFAAAGKSVRKKDLGLM
                     AMSYGYVYVAQIAMGANMNQTMKAILEAESYPGPSLIIGYAPCISHGIKSGMGTSMMQ
                     EKKAVEAGYWHLYRYNPALRAQGKNPFILDSKDPAQDFQDFITGEVRYTQLMNVFPER
                     ADTLFELAEQHAKERGARYKRLAEEEVL"
BASE COUNT         3010 a         2355 c         2079 g         2871 t
ORIGIN      
        1 ctttttttct gtattttttt ttttaaaaaa taaacggaac tatggtgtta acacttgcct
       61 gtcatcaaaa aatgtctgct ttttgtgggc taatcatact ataagtaaga taaaagtttg
      121 gcttgcagtt ttgcctgcaa acttttatcc tgcttcattt agaagacacc tgccaaacgc
      181 aagcgatata ttatgtcagt actaatgcgt cacttaagat cgacttttta tgtggaggga
      241 ttttcgtgaa aaagattgct ttagttaccg atagcacagc cgacttaccg ccatcattag
      301 ctttagaatg ggacgttcat gtaattccct taaagatctg ttttgctgcc gatgaatttt
      361 acgatggtga actggcggca gaagattttt atcgacgttt gcaaagcgca aaagaattgc
      421 ctttttcttc ccagccggca ccagaagatt ttttgaaact ttatcaatcc ctgcttaata
      481 agtatgacga agtgatttca attcatttat cctccaacct aagtggcacc gttaatgcag
      541 cttgggtagc ggcagaaaca ctccagggaa aagttcatgt tattgactct aaaactatta
      601 gtttaggtat tgctctgatg gtaaatgaag ctgtacgctg catacgtgat ggttttgatg
      661 ccttccaaat caaagaacgt atggacaccg cacgtaagaa tattgaaact atgttcaccc
      721 tagataccct tgagttcctg caaaagggtg gtcgtatcgg taaagttaca ggtctggtag
      781 gttcgctact taatattaaa ccggtaattc gtgttaatga ggagggcatc tatattcctg
      841 caggcaagac caggagtcag gataaatcac tgcagcaaat tgtgcaaggc ttacaacagt
      901 tagccaatgg gcgtaaaata aaagcgcttg ctgttgcaca tggcgccgct ctagaagcgg
      961 cccaaagact taaagatgct ctggaaaaaa cctttaatat ccctgcctgc atgttcactc
     1021 aagttagcac tgttattggt gtacatacgg gccctggcac tgtaggtacc gctgtacatt
     1081 ttgagtagaa gtaaagagaa tacagaagct agcaaaaagt aaccatgacc agtggttact
     1141 tttttgtatg cagggcagta actgcagaaa ttttctaact gatctgccgg ggtaaagtga
     1201 cagtggttac attaaatggt atgatcatac actattgaca atgatttacg tttatgttat
     1261 catttatata tgggtgccac gcctaaaggc gggtgcccac atcctttatc taaaactttg
     1321 cataaatagc tatctggagg aagtaaaatg cagaccgcaa tctcaaaagt tagactcgat
     1381 catctcgaaa aacatatagc agtaattaaa gatgagcgaa aacagacact agaacagtat
     1441 ttcaccaagt caattaatga gcgtaatgac tttaacgatt ttcttgacaa ctacctgcaa
     1501 catatcgaaa aattcgtaaa atctgctaac aatgctcagg cgcttaacct gcaagaggca
     1561 cctttcattt ttattggttg tgatgtggaa gttgaagacc tggacactgc agacatcatt
     1621 aactaccatt taactgatcc tattcaggag cgtgtcggag aagacgacgt atcttattta
     1681 tcacctgtag gcaaagccct tttgctaaaa aaagtcggtg atgaaatcca agttaaaacg
     1741 cccggtggga caattcgtta taagatcaga gccattcact tcaaagcata aaaaaacgca
     1801 ttagaaaata caccaaaggc cgccatcaac aagatggcgg ccttttgcag ccaaactatt
     1861 tattttctcc agaaactggg gaaaaccaaa gaaatcatgg tgtaaagttc caagcgacca
     1921 aaaagcatac aaatagataa gatatttttg ccaaagtctg acaatggggc aaaggtttta
     1981 gtaggaccaa aagcacttaa ccccggtccg atattaccaa gggtacaggc agctgctgag
     2041 acggcgctaa tcatatccag attttgtgtc aaaagcaaca acgttgccac aataaaaatt
     2101 aaaaaatata gtagggaaaa cgccattaca ccagacaaca cagtctctgg tatagtctca
     2161 tcattaacct taacagcttt aatcgccctg gggtggatta aagtgccaat ttctctttgt
     2221 atatatttaa agatgaggtg gattctaata accttcatgc cgccaccggt tgaaccagcg
     2281 caagcgccaa taaacattag tgttaataat agcgtgcgac ttaagtccgg ccataaatca
     2341 aagtcagtag tggcatagcc agtggtggta atgatggacc ctacttggaa agaggcatgc
     2401 cgcaggcttt gtgcaaagga attaaagttc gggccataga tattaaaggc aataactaaa
     2461 atataggcag cgataatgcc cagatagact ttaagctccg tatcctgcca taattctttc
     2521 aagcttcttt tcttaaaagc caggtaatat agggaaaagt taacacctga caaaatcata
     2581 aatgttgtaa tcacaacttc aaagtaaaca ttattatagg cgccaatact agaatttttt
     2641 atggagaaac cgcctgtacc taaagcgcca aaagtatgac acaatgtatc aaacagcggc
     2701 ataccgccca gccacaaaaa caaaatcatc agcacagtga ttgccagata agttatatat
     2761 aatattttag cagtatcgct aactttcgtt gatagtttag aggaaattgg acctggactt
     2821 tcagctttta aaatctgaaa cgtaccaatg cccaaggcag gactaagggc cagtgttaaa
     2881 actaaaatac ccattccccc cagccagtgc gtaaaagaac gccaaaacag gaggcaattg
     2941 ggtaggccct ccacattagg tatcaccgtg gctcctgtcg ttgtaaatcc agaaactgtt
     3001 tcaaagaaag catcgactgg tgaggcgagg gctcctgaaa ataaaaatgg cagcgcgcca
     3061 atgatggaag caataatcca accaaagcta acgatcgcaa atccttcttt ataacgaatc
     3121 atcttttctt tgggtttaat taaatatagt aaaaaaccaa caaccgctgc tatcaaaatg
     3181 gtgatgataa atgcggagat tgccgtctcc ttataaataa tagctactgc cacagagggg
     3241 agcatagcta cagactcaca aagaagcaat atccccagag tttttagcac tattaatgag
     3301 ttcattaata ggaacccccc ctccgttggt gttaaagagc ttcttcactt tttctatcag
     3361 gctttctaaa gtaaagacaa ttacacggtc tgtactttta attactgaat caccattagg
     3421 aataataatt tgttcattcc taaaaatagc accaataatt actccttttg gtataccaat
     3481 atcttttagt ggcttgttaa caatcggcgt accttcctgg gggataatct caaagacttc
     3541 agctcgacca ccgattaaga gtgacatgga aagaatttta ccgccacgga taattctcag
     3601 gatatcccta gctgtaatta atttcggact cacagcacta tccaaaccaa ttgtttccac
     3661 aacagaatca taactggagc ggctgacctt agcgattact tttttcgcac ccaactgctt
     3721 ggccaacatg gaaactaata aattctcttc atcataaccg gttaaggaga caaaagcatc
     3781 catttcatca atattctccg cctttaataa agcaatatca gtgccatcac catggagaac
     3841 aagcgtatta tccaaccctt ccgctaattc ccgacaacga tgcaggtttt cttcaattat
     3901 cttgacattg ataccaagct gctcaagttt tcttgccaaa tagaaagcgg tcttgccgcc
     3961 accaataatc agcacattac gaactttact attgtcgata agcaccccca tgcttctggc
     4021 aaaggcatcc accgcagctc tctcacccat gatgtagatt gtatcttgag gataaatata
     4081 atcgaagccg ttaggaataa ttacattacc attacgggca attgccgtaa taattactga
     4141 ttctgggagg tcgatatctt ttatttgttt gttgcttaag ttagccgtgg cattaatcgg
     4201 cacttctgaa attcttactc tgcctttggc caaatctacg gcataggagg tatgagaatt
     4261 aagcagcaaa cgcaaaattt cctgggcagt agcatattca ggattaatta tgcattcaat
     4321 atccaggttt ttctgcataa aatctaattc cgctacatac tcagggtttc tgacacgggc
     4381 gatgacgcta tttgtaccta gccttttagc agtaatacag gagacaatgt tggcctcatc
     4441 gctattagtc acagcaatta agaggtcggc gctggaacaa tctaattttt ctaacaaacg
     4501 actcgatacg ccattacctt tcatcgccag cacatcgaag ttatcgttta atttatcaat
     4561 aacattttgg ttattatcta ccacagttac atcatggttt tcattaacta agctctcaac
     4621 taattgatag cctaccttcc ccgccccgat gatgatcacc ttcatagcaa accctccact
     4681 tttccttttt tccagtatca agcctagttt actcctcttt atagggaaaa gcaatagccc
     4741 ctattttagc taaaactttg ttcggttgct gctgagaaaa tatagttttt ttgtgtccct
     4801 tatttcataa gattatccta ctcctgccac tattcacaat acttctccac aatacttccc
     4861 tgcgaatctt ttgcttgcaa ttacgccttt tctaaagtat tatagataaa ataacatgtg
     4921 attattatca catgcttcac aaggggtgaa cccaatgtgg taggcgcaat caggcttgta
     4981 atccagaata gcctttggta tcatagtttt aatacataga gtaaagtggt aaatcattaa
     5041 cactttacag cgttaggagg aaaaagaagt gaaagtttta gtcagcgatt ccatctctga
     5101 acttggtgtt gccaaattac aagaaaagat tcaagttgat gtgaaaacaa agctcacaga
     5161 agaagaactt atctccatta ttcctgagta tgatgccctg attgtgagaa gtgctaccaa
     5221 agtaacgcgt ccgattattg aagctggtaa aaaactacag gtaataggtc gtgctggtgt
     5281 gggtgtagat aatattgata tagaggcggc cacagaacgt ggcgtcatcg tcattaacgc
     5341 ccctgaaggc aatactattt ctgcggctga acatactatt gcgctgatga cggccttagc
     5401 ccgcaacatc cctgctgctt cccgttcatt aaaagcagga gaatggaaac gcagcacctt
     5461 catgggtgtt gagctttata aaaaaacatt aggtgtcatt ggtattggtc gtatcggcag
     5521 tgaagtaatc aaacgtgctc aagcaatggg catgaacatc ctagcttatg acccctttat
     5581 ctctgtagag cgtgctgaaa agttgggtat taccctgacc agcccagaag atatttaccg
     5641 ccaagcagat ttcattaccc tacacacacc aaagaataat actacctttc atatgatcgg
     5701 cgaagaacaa atcgccatga tgaaagatgg tgtgcggatt attaactgcg cacgtggcgg
     5761 gctgatagat gaaaacgccc tctgcgctgc cctacaaagt ggaaaaatcg ctggcgcggc
     5821 actggatgta tttgaagaag aaccagccgt agctaacccc ctctgtgcac tgcccaatat
     5881 tattgttact ccccacctcg gtgcctccac agaagaagca caaatcaatg tggccgttca
     5941 agtagcagag cagatagtta gcattcttgc tggtgaagca ccgctcacag cgattaatgt
     6001 tccttcgatt ccaccagaaa caatgtctga agttaaaccc tttattccgc tcatggcaac
     6061 actaggttcc ttttatgcac aagtctttgg tgggcaggtt gaggccatag atctcttcta
     6121 taatggtgaa attgccaact tcactgttgc acccctaact acttctttcc taattggctt
     6181 cttatctact ttcctcgagg atactgtcaa tgccgttaac gctccggtgc ttgctaaaca
     6241 gcgcggcatc aaagttcgtg aagtcatcag cacttctgta gagaacttta ccagcctagt
     6301 cacagtaaat gtgaaacagg gtgctgaaga gcacacccta gccggcacac tctttaacaa
     6361 taacgatatg cgtattgtgc aaattgataa ataccgcatt gaagtaattc cctcacgttt
     6421 catgctggta actaaccatc tggacgttcc cggtgtcgta ggcaattgcg gggtaattct
     6481 cggagaagaa aacattaaca tcgctggcat gcaggttggt cgacaatcca tcggcggtga
     6541 agccgtgatg gtcttgcagg ttgatagcac agtaccagaa agtacgattg ccaaggtgca
     6601 aaacctcgaa ggcatgttct ctgtgaagtt tgtgcagctc aattaaatat tttaccgaat
     6661 aaatagagga aggcctatgg ccttcctcta ttttatttat ttataacact tcttcttcgg
     6721 ctaaccgctt atatctagcg cctctttctt tggcgtgctg ttcagctagc tcaaacaagg
     6781 tgtcagctct ttccgggaaa acattcatta attgtgtata acgaacctct ccggtaatga
     6841 aatcttggaa gtcctgtgca ggatctttgg aatctaagat aaacgggttt ttaccttggg
     6901 cgcgcagagc tgggttgtaa cgatagagat gccaataacc tgcttccacg gcctttttct
     6961 cctgcatcat actagtgccc atgccacttt taatgccatg actgatacag ggcgcatagc
     7021 cgataatcag tgaaggtcct ggataacttt ctgcttctaa gatcgccttc atggtttggt
     7081 tcatattagc acccatggca atttgggcga cgtagacata accataactc atcgccatta
     7141 aacctaaatc ctttttgcgc acacttttgc cggcagcagc aaatttagca acactacctg
     7201 taggggtagc tttagagctc tgcccacctg tattggaata tagttctgta tccatcacaa
     7261 agatatttat atcttcgccg gaagctaaaa catgatctaa accaccgtag ccaatgtcat
     7321 aggcccaacc gtcaccacca atggaccata ctgatctttt agttaggaaa tcctgtctac
     7381 ctttgatttc cagcagtaag ctgttttctg gactatttac ttggtctaaa gctgcgcgta
     7441 ctttggcggt tgcagctttg gcggcctcgc cattatcttt atctttgagc cactgtgtca
     7501 aggcttcttg caactgtggc ggaagctctg tttgcagcgc ttcctcgatc aacgctgcca
     7561 gtttttctct tttttgcagt acgccgacca acataccaaa cccgaactct gcggcgtctt
     7621 caaataaagg attaacccag gttggacctt taccttctgc attcgtagta taagcgatgg
     7681 aaggagctga agcaccccag atagaggaac agccagtggc attagaaatc atcatccggt
     7741 cgccaaagag ctgggtaagc aagcgaatat agggtgtttc accacagcca ggacaagctc
     7801 cgctgaattc cataagcggc cgcacaaatt ggttaccttt aacggtatgg gggttaagca
     7861 aatcatcttt ggtggtcaca gtcatggcaa actcccaatt gtctgcttct ttttccgtca
     7921 cctgcgccgc gggttgcatg attaaagcct tatccggtgc cggacagata tccgcacagt
     7981 tgccgcagcc cgtacagtct aagggtgaaa cctgcacgcg atactgcagg tgcccaatac
     8041 ctttacccac agcttttatg gtttgaaaag tttccggagc acgctctgct tcttcttcat
     8101 ctaacaagta gcctctgatg gtggcatggg ggcaaacata ggtacactgc ccacattgaa
     8161 tacacttgtc aatttgccat tctggcagct ggggagcaat accgcgtttt tcataagcag
     8221 ttgtgcctgg cgggaaagta ccatcctcca tgccggtaaa agcactgacg ggcaattcat
     8281 cgccttcctg cctggccatc ggtcgctgta tgttctttac gaaatctggc tcatctttaa
     8341 caggcagctt ttcatctgct gctgtctgcc actcctgcgg cacatctact ttaattaagg
     8401 cttccagggc cttatcgaca gccgcatgat tcatcttcac aatgttttcg ccttttctac
     8461 catacatctt aacaatagaa tctttcagta agcgcagcgc ttcttcaaca ggaataactt
     8521 ccgccagctt aaaaaataca gcctgcatca ccatattgat tctattgccc agccccactt
     8581 gattagcgat agccaaggca tcaataatat agaaactaat ttcgttttta gccaaatact
     8641 gtttcaaggc tgccggcagt tgcgctgcta cttcttctgc ctgccaggca cagtttaaaa
     8701 cgaaagaacc gccttttttc aagcctttga ccaagtcata atgatgaagg aaggctggat
     8761 tatggcaagc tatgtaatca gcatcaaaga ctagatacgg tgcctgcacc ggcttttgcc
     8821 caaatcttaa gtgggaaacg gttgtaccac cggatttttt actatcgtag gagaaatatg
     8881 cctgaacatg cagatccgta tggtcaccaa tgattttaat tgctgattta ttagcgccaa
     8941 ccgtaccatc tgaacccaga ccccagaact tacagctgat ggtcccagcc ggtgtcgtat
     9001 taacaatctc ctcttccgca agcgaagtat ggctaacatc atcgataatc ccaatggtaa
     9061 agttatcttt tggttgcggt tggtttaaat tgttaaaggc ggccataatt tgtgacggac
     9121 gggtatcctt tgatgataag ccataacggc cacccacgat tactggtttc tgcgcagcat
     9181 tttgcagtgc tttgaccaca tctaagtata acggttcacc gggagcgccc ggctctttgg
     9241 tacggtctaa aactgctatc tttttcaccg tggccggcag cgcttggaga aaatgctcta
     9301 cagagaaagg acggtaaagc cgtactttca cgacgcctac tttttcgcca cgcgcaagca
     9361 aaaagtcaat ggtttcactg atcgtatcac agacagaacc catcgcgaca atcactcttt
     9421 cggcatccag tgcgccataa tagtcaaaga gctgataatg acgacctgtc atttcaccaa
     9481 attgatccat atagttttgc acaatattcg gcacggcggc aaagaaagga ttctgacctt
     9541 ctctttgttg gaaatagata tcagggtttt gcgcggttcc tctgaccaca ggatgttccg
     9601 gatttaaagc acgctgacga aatgctgcta gtgcttcttg atccacaagc gctttgatgt
     9661 cctcatattc aagaacttgg atcttctgaa tttcatgcga ggtacgaaaa ccatcgaaga
     9721 aatgaataaa cggtagtctg gccttaatcg ccgctaaatg tgcaagacaa gctaaatcca
     9781 tgacctcctg cacattggag gaagctaata aagctactcc agtctgacga caggccatca
     9841 cgtcttggtg atcaccaaaa atagataaag catgcgtagc aattgacctc gccgccacat
     9901 gcagcacccc tggtaataat tcacccacca ttttatacaa actaggaatc atcaataata
     9961 agccctgcga ggccgtataa gttgatgata gcgaacccga ctgtaaagcc ccgcgcatcg
    10021 cagccgccgc accagcttcg gactgcattt ctaccagctt aacgggttgg ccaaagatat
    10081 tcattttacc atgtgcagac cattcatcta ccccttcggc catcggcgtc gaaggcgtaa
    10141 ttggataaat tgcggccact tctgtaaaag catatgaggc atgagcggct gcctggttac
    10201 catccattgt ttcaattttt gccattactt aactcccctt cctcttaacg tcgctaactt
    10261 tattattata atcacggctg attataatgt atcaaaaaaa ccctaacaaa tgtat
//
LOCUS       DASRMS010000019         9145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_674451, whole genome shotgun sequence.
ACCESSION   DASRMS010000019 DASRMS010000000
VERSION     DASRMS010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_674451"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(120..254)
                     /locus_tag="VFC74_01015"
     CDS             complement(120..254)
                     /locus_tag="VFC74_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23951.1"
                     /translation="MKMLSAGMSGVSEDKKQGQMPVMVQMQTIIKERYIGTALSVLLG
                     "
     gene            442..660
                     /locus_tag="VFC74_01020"
     CDS             442..660
                     /locus_tag="VFC74_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23952.1"
                     /translation="MKYQGFILDKDGRPSVCPRCSNEELNGEYCKICGTYIVNKCSAE
                     TDLDLNGNEYVTKESCGAPAAYSGTSGR"
     gene            complement(818..1066)
                     /locus_tag="VFC74_01025"
     CDS             complement(818..1066)
                     /locus_tag="VFC74_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04540 family protein"
                     /protein_id="HZK23953.1"
                     /translation="MEFLLYPTTVKMLAAEINLACDAYISRKITAQELQTVIWHYAKH
                     YPKMLFNAQDLNPTVLNRIGKKRALLVNKMLEGYQYRL"
     gene            complement(1154..1912)
                     /gene="istB"
                     /locus_tag="VFC74_01030"
     CDS             complement(1154..1912)
                     /gene="istB"
                     /locus_tag="VFC74_01030"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZK23954.1"
                     /translation="MLNNPTIEKLKDMKLKVMAAMLADPDPTLRELSFEERLGLMVEK
                     EWLARKNSRIKRLLRAATLGLDACVEDIYYSASRTIDKKTVQTLSTCAFMERKLNIVI
                     SGKTGSGKSFLACAFGNKACRQGYHVKYYRIPELLLEIQEAKNEHRYSKFMDQLQKVK
                     LLILDDIGLKSYTLEESRDLLEIFECRYNKASTILSGQVSHTKWYDLFPDPTIADAIM
                     DRIIHNSYILALDSRQSMREVMAKKNVATISETD"
     gene            complement(1905..3440)
                     /gene="istA"
                     /locus_tag="VFC74_01035"
     CDS             complement(1905..3440)
                     /gene="istA"
                     /locus_tag="VFC74_01035"
                     /inference="COORDINATES: protein motif:HMM:NF033546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZK23955.1"
                     /translation="MRRLEMLKAREILRLKFEAGLSVREIGKACNCGKSTVSELLKRA
                     QEAEITWPIELTDKQLMSALYPPAESRTSPPEPDMEYIFREMKKKNVTLLLLWEEYKE
                     KHSDGIMYTQFCDRYRKFKKQNNLSMHKEHKAGEEAEVDWAGTTMSYIDPITGEEKAA
                     HIFVSVLPASNYPFAYAYGDEQLPSWIDAHVRAFEYYGGVPKITISDNTKTAVLTPDL
                     VDPVLNRSYHEMARHYGTTLLPARAYKPKDKGADEGMVRIVSNRVLAALRNRQFFSLQ
                     EINEAIKEELVKLVKRPFQKREGNRQTAFEAIDKPCLQPLPKTKYEYGEWKEAKIQFN
                     YHVDYAGFFYSCHYSYVNQRCSIRATARMIEIYIGTERVAAHPRNYNTYKRYTTLAEH
                     MPEAHKAVSGWSSDRYLSWAEKIGPNTRELVRNILESREYPVQTYRACMGVMRLSKSY
                     SAELMEKASREALEKNLCSYKYFEIMLKQAAKSPVKKEKEKIIQHENVRGSSAYQGGG
                     LRA"
     gene            4434..5801
                     /locus_tag="VFC74_01040"
     CDS             4434..5801
                     /locus_tag="VFC74_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23956.1"
                     /translation="MKNLQFRVFCIIILLMIIATTISACGQPRPISESSLDMNISFSD
                     DVVFGAGSSGFTYSVCEQGLFYHKDNKLQYYDFAAKEKYVLCDKANCNHANASCSAWY
                     ENVHDATGLAMYGEKVYVIKLNSNSNKYELLSMNPTGNNQKVIYSLDIGDYRADSWVL
                     ESIRYVYYAGGMAWLSADYQYIGEPAEESSDFSDSAHTLIIGINLQDGTSITLNELSE
                     AKAAYQLELVAKDYIVLRKDWQALEQLSQKAFAEAYEQGEFAEFSSAADPYYEYQVWY
                     QDHSEPMYLYLIYDLKTGEVIEFERGKHKLNYDEDGHVGGLLPKYIFTGTYDGKLIYI
                     ISDYEKNGSLYLLDVGNNVSEQILEIENGGTLSGIIDNDKILYCLYTEQEKADILYYD
                     LQTKEHVALFQDDRRITFRLIGETSTKLIGIIYNPITYNPTVYSIDKEDYYQGNLNAA
                     EKLRL"
     gene            5816..6589
                     /locus_tag="VFC74_01045"
     CDS             5816..6589
                     /locus_tag="VFC74_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013724668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK23957.1"
                     /translation="MVKLTIDSLRKNYGQKEALKGVSFTLSQGIYGLLGPNGSGKSTL
                     MNILTGNLKATSGTVLWNEQKIAKLGKKYREILGYVPQQQALYPDFTAIRFLSYIAAL
                     QGLPAKEAKKKISSALAVVELTKVANKKIKTLSGGMKQRLLIAQSILAEPKLLIMDEP
                     TAGLDPKQRVLMRKLITNLAADKIVLLATHVVADVEWIADEILLLKDGFLIRKGSRGA
                     LVEEVQNLHPSLQATEKQLGLEDVYLHYFGEQDHETDLL"
     gene            6570..8279
                     /locus_tag="VFC74_01050"
     CDS             6570..8279
                     /locus_tag="VFC74_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23958.1"
                     /translation="MKLIYFEVCKLFQRRAAWSVIVGLIFANALLIHYQINCVNEAGY
                     SSKDIAVVYEKVKGKNSQEQLSWLEAEVARYIQGVQAGDERYTYGQDRTLILVYEQIA
                     AVLDYDHYLANIHKQAEKMMKSALFAAPDTFSYRNVVLTPQAYAHLKGLEVTADFSDG
                     VLLITEHRLTDAFLLLALVILTMHLLISEREEGTLPLVKATKYGYGSTIFAKAVVLLL
                     LTLFLALIFYSTNLIVATGEVGLGDLNRPIQALAGYRSSPLKITVKQYLGLFFSAKLL
                     GVLANLYIFFLLGILCRNHLFSCLLGILVLGLEALLYKNIDLHSWLSPVRQCNLTALL
                     DTGSYFSNYNNLNLFGYPVNTITAGVVTGFTAIIFGVVMGIFVFSKEAASDMRRNWLK
                     EKLTANITFFKGLHFNLLRHEAYKLLIMNKGLLLILIFIVSQVFYFNGMMYFIDQEEF
                     YYQNYSTMLAGGLTEEKNAFLQNEQERIAASEQERELLYQRYENGEIDERYLYYCLSQ
                     LEVNGFKKDAFEKAQTQYHQLIAMQEKKQPVAYVYQTGWHKLFGQEAKRLDLLEYDNF
                     FCS"
     gene            8315..8695
                     /locus_tag="VFC74_01055"
     CDS             8315..8695
                     /locus_tag="VFC74_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23959.1"
                     /translation="MTFMHQLISVSKKGRKAVRNRKILICVCFAMISAAISFLPRTIR
                     VFQTYGASGLSAPAKSIMEIPWAPNGLSIANFFILYQVMRLMIAALAGVFILVASHKT
                     GNTITTILISTAVLILPLATMLLA"
BASE COUNT         2537 a         1907 c         1975 g         2726 t
ORIGIN      
        1 aacagaccac tgcaaagctg catactgagt taactccact aaggccacct tctttttacc
       61 ttctgccgcc aaataattgg ccaccgcagc ggccatggtc gtacagccaa cacctcttct
      121 taccccaaca acactgataa agccgtgcca atatatcttt ccttaataat cgtttgcatc
      181 tgcaccatta caggcatctg cccctgcttt ttgtcttcac ttaccccact catcccagcg
      241 gacaacatct tcatatgttg cagcttcttg ttcgagcatt acctccaata tattgatttt
      301 atctccaagt ctctttcttc ggacatttct ggagccaaga tatcataaat ttgtactaat
      361 tgtggtttct gtctagtttc tgagaaaaaa caacattgcc cgctatgtgg aaacatactg
      421 gcatagggag aaggggaagt agtgaaatat caaggattca ttctagataa agacggccgt
      481 ccatccgtct gcccgcgctg cagtaacgaa gagctaaatg gggaatactg caagatctgc
      541 ggtacatata tcgtcaataa atgttcagca gaaacagatc tcgacttgaa cgggaacgaa
      601 tatgttacaa aagaatcatg tggagcacct gctgcatatt ccggcacatc cggacggtga
      661 atccggaggc atccggacag caaaccggaa ttattcggac accttgtcgg ttgatctgat
      721 tcgctattaa gtttttccga aggcgatctt ttcttgcacc cagtgatagg gctaatgttg
      781 tccgaaggct gcagatccta ataatcaaaa aatcacttta aagtctgtac tggtagccct
      841 ctagcatttt gtttaccagc agtgcccgct ttttgcctat cctgtttagt actgttgggt
      901 ttaggtcttg ggcattaaag agcattttgg ggtagtgttt ggcataatgc catatgactg
      961 tttgcagctc ttgggcagtg atctttctgg aaatataggc atcacaggct aaattgattt
     1021 cggcagcgag cattttaact gtggtgggat acaacaaaaa ctccataaag cacctcgtgg
     1081 aatcgtaata ttctgtacta gcccagttgt tttcgtacca agattgtaca ctagattcga
     1141 cagccacgca atgctaatct gtttctgata tcgtcgccac gttcttcttg gccatcactt
     1201 cccgcataga ttgtctggag tccagggcga gaatgtagga attatggatg atcctatcca
     1261 taattgcgtc cgcgatagtg gggtcgggga ataagtcgta ccactttgta tgagagactt
     1321 gccctgacag gatggtcgag gctttgttgt acctgcattc aaagatttct aaaaggtctc
     1381 tgctttcttc cagggtgtac gattttaggc caatatcatc cagaattaac agcttaactt
     1441 tctgtagctg atccatgaat ttactgtatc tgtgttcgtt ttttgcttcc tggatttcca
     1501 aaagcagttc cggtattctg tagtatttaa catgataccc ctggcggcag gctttgttgc
     1561 caaaagcaca ggccagaaac gatttaccac tgccggtctt gccagagata acaatattta
     1621 atttccgctc cataaacgcg caggttgaaa gcgtttggac tgtcttttta tcgatggttc
     1681 ggcttgcgga atagtagata tcttcaacgc aggcatctag tccaagggta gctgcccgca
     1741 gcagcctttt aattctggag ttcttccttg cgagccattc cttttcgacc atcaggccta
     1801 gcctttcctc aaaagataat tctcgcaggg tggggtcggg gtcagccagc atggcggcca
     1861 tcactttgag tttcatgtct ttaagcttct caatcgtggg gttgttaagc acggaggcca
     1921 cccccttggt aagcactgct tccccgaaca ttttcatgtt gaatgatttt ttccttttct
     1981 ttctttaccg ggcttttcgc agcctgtttg agcataattt cgaagtattt gtaagagcat
     2041 aaattctttt cgagagcttc acgactggct ttttccatga gttctgctga gtagctttta
     2101 cttagccgca taacccccat acaggcccgg taggtttgga ccggatattc gcggctttcc
     2161 aaaatgtttc tgacaagctc gcgggtattg gggccgattt tctccgccca agacaaatag
     2221 cgatctgagc tccagccgct gacagcttta tgtgcttccg gcatatgctc cgcaagggtt
     2281 gtgtatcgct tgtacgtgtt gtaattcctc ggatgtgcag cgacccgttc cgtgccgatg
     2341 tagatctcga tcattctggc ggttgcacgg atggagcagc gttgattgac ataggaataa
     2401 tggcaactgt aaaagaaacc agcgtagtca acatggtagt tgaactggat ttttgcctct
     2461 ttccattcgc cgtactcgta ctttgttttc ggcaacggct gcaggcaagg cttatcaatg
     2521 gcttcaaatg ctgtttgtct gttgccctcc cgtttttgaa atggccgctt gaccagctta
     2581 acaagctctt ccttgatggc ttcgttaatt tcttgcagac tgaaaaactg cctgtttctt
     2641 agtgcagcaa gaactctgtt tgaaacaatc ctaaccatgc cctcatcagc tcccttgtcc
     2701 ttaggcttgt aagcccgtgc tgggagcagt gtagttccgt aatgccttgc catctcatga
     2761 tagcttctat tgagcactgg gtccacaagg tccggagtaa gaacggctgt tttcgtattg
     2821 tccgagatgg tgatcttcgg gacacccccg tagtattcaa aggctcgtac atgcgcatca
     2881 atccagcttg gcagctgttc atccccgtaa gcataggcga aaggataatt gcttgctggc
     2941 aggacagaga caaagatgtg agctgccttt tcctctccag tgatcggatc gatgtaggac
     3001 atcgtggtgc ctgcccaatc cacctcagct tcttcacctg ctttatgctc tttgtgcatg
     3061 gagaggttgt tctgcttctt gaatttcctg tagcgatcgc agaactgtgt gtacataatt
     3121 ccatcggaat gtttttcttt atactcctcc caaagcagta aaagcgtcac attctttttc
     3181 ttcatctcgc gaaagatgta ctccatatcg ggttcaggag gtgatgttct gctttctgcc
     3241 ggtgggtata gtgctgacat taactgcttg tccgtaagct caatgggcca tgttatctcg
     3301 gcttcttgag cccttttaag cagttccgat accgtactct tgccgcaatt gcaggctttt
     3361 cctatctcac gcacagatag tcctgcttca aatttcaacc gtagtatttc tcttgctttc
     3421 agcatctcca gtctcctcat cgttggcccc cttaatctct ctttgattaa agagtaccag
     3481 tttttttatc tactcgacaa ggtgtccgga tgtgccgtta tgctgtccgg ataattccga
     3541 aacgctgtcc ggatgtttcc ggaatgggtg tccggatgcg gccgaaatac gcacctgctg
     3601 aaggaaatgc aagatattgt attcactgcg gaaatcgtac tacttattac ataaatggtt
     3661 tactcccctc ctggaatgaa gaaaaagttt agacgaggtg aaagactaat tccagaaaag
     3721 attccgctgg gttctcatca tgggaacaat aaaagttaac gacatagcat gcaaaaagtg
     3781 caaaaaaata cgacccgctg ataataaagc ggatcatgag cttaactcgc catattggaa
     3841 aataacacag ttataaaccc attgactaca ttcgaaacgt cgcttatacg gcaaatatga
     3901 gcgtcgaatg ttgagaggaa taccctagtt taatctgttt gtggggtttg ccgcataaaa
     3961 atgaaaatac ccccttatgt agcggtaaat ttataatcta aattagggat taaggtaggc
     4021 ttattaacta ttgtaataaa aaggggttac aggtgattat ggatataatt ataggatcct
     4081 ttgtaactct acacattaaa ctacaattac cggcaaaagg ttacaatatt catcgaagga
     4141 gtaatgctct ttgctgttga atatattgct gtaagtaaag acagtatgta gtcaggactg
     4201 catttagaag aatgcagcaa acagtgaata gctgcccctt tcccttcccg atcactgaac
     4261 caggcggaga tggagttatt tatagagatt cacaaggtgg ctgacattta ctacaaccgc
     4321 cgctgctgca ccagtattaa tgattaactt taaagataca gattattgat gcaatattcg
     4381 cagttagcag aaatatcagg cagtaatatt caaagaaaga gggaggaatg gaaatgaaaa
     4441 atctgcagtt tagggttttt tgtatcatta tccttttaat gatcattgca accacgatct
     4501 cagcctgtgg gcagcccagg cccatatcgg aatcttctct tgacatgaac atttcttttt
     4561 ctgacgatgt agtctttggt gctggctcat ctggttttac ttatagtgtt tgtgagcagg
     4621 gtctctttta tcataaagat aataaactgc aatattatga ttttgcagca aaagaaaaat
     4681 atgtactttg tgacaaggca aattgcaacc acgcaaatgc aagctgcagc gcgtggtatg
     4741 aaaacgtaca cgatgctacg ggtctggcca tgtatggaga aaaggtatat gtaattaaat
     4801 taaattcaaa tagtaataaa tatgagctgc tgagtatgaa tcctacaggt aataaccaga
     4861 aggtaattta ttctttagat attggtgact acagagctga ctcctgggtc ttagagtcaa
     4921 ttagatacgt atattatgca ggtggcatgg cctggctgtc agcagattac caatatatcg
     4981 gagaacccgc agaagaaagc agtgacttta gtgattcggc gcataccctt ataattggta
     5041 tcaacctcca agacggaaca tctatcacac taaatgaatt atctgaggcc aaggctgcat
     5101 atcaacttga gcttgtggca aaagattata tagtgctacg caaagattgg caggccttag
     5161 aacaattgtc acagaaggct ttcgctgagg cctatgagca aggcgaattt gctgaattca
     5221 gcagtgcggc agacccctac tatgagtatc aggtatggta tcaagatcac agtgagccga
     5281 tgtatttata ccttatatac gacctgaaga ccggggaagt gatagaattt gagcggggaa
     5341 aacataaact caattatgat gaggatggcc atgtaggggg acttctccct aaatatatct
     5401 ttacaggtac atacgatggc aaattgatat atataatttc agattacgaa aaaaatggtt
     5461 ctttatattt attagatgtt ggaaacaatg taagtgaaca gattctggag atagaaaatg
     5521 gcggaactct ctcaggtatc attgataatg ataagattct gtattgtctt tatactgagc
     5581 aggaaaaagc agatattctt tattatgatc tccaaacaaa agaacatgtt gctttattcc
     5641 aagatgatag aagaatcacc ttcagattga ttggtgaaac ttccactaaa ctgatcggca
     5701 ttatttataa tcccattacc tataacccca ctgtttattc aattgacaag gaagactact
     5761 atcagggaaa tttaaacgct gcagaaaaat taaggttata gagggagtta aaagcatggt
     5821 caagcttact atagatagct tgcggaaaaa ttatgggcaa aaagaggcct taaaaggggt
     5881 cagctttacg ctgtctcagg gtatttatgg cttactgggg ccgaatggtt caggcaaaag
     5941 cacactgatg aacatcctta ctggaaactt aaaagccact tcaggcacag tattgtggaa
     6001 cgagcaaaag atagctaagc tcggcaaaaa gtatcgtgaa attttgggct acgtgcctca
     6061 gcaacaggca ttataccccg attttacagc aataaggttt ttgtcttata tcgcggcact
     6121 gcaagggtta ccagcaaaag aggcaaaaaa gaagatctcc tctgctttgg ctgttgttga
     6181 gttgactaag gtagcaaaca aaaaaattaa aacgttatcc gggggcatga agcagcgtct
     6241 gctgattgcg cagtccatct tggcagagcc aaaattgctt attatggatg agccgactgc
     6301 aggtttagat cccaaacaac gtgttttgat gagaaagctg attacaaatc tcgctgctga
     6361 taaaattgtc ctgctggcca cccatgttgt cgccgatgta gaatggattg cagacgaaat
     6421 tttactgtta aaagatggct ttctgatccg taagggcagc agaggggccc tggtagaaga
     6481 agtacaaaac ctgcatccgt ctctgcaggc gactgaaaaa cagcttggcc tggaagatgt
     6541 ttatctacat tatttcgggg agcaggatca tgaaactgat ttactttgaa gtctgtaagc
     6601 ttttccagcg cagagctgcc tggagcgtga ttgtcggctt gatctttgct aacgccctcc
     6661 tgattcatta ccagattaat tgcgtaaacg aagcaggata ttccagcaag gacattgctg
     6721 tagtctatga aaaagtgaag gggaaaaata gtcaggaaca gttgtcatgg ctagaggctg
     6781 aagtagctcg ctatattcag ggagtacaag ccggagatga aaggtatact tatgggcagg
     6841 acagaacttt aattctggtc tatgaacaaa tagcagctgt tttggattat gatcattacc
     6901 tagcaaatat ccataaacag gcagaaaaga tgatgaagtc tgcacttttt gccgcgccgg
     6961 ataccttttc ttatcggaat gtagtactga caccccaggc gtacgcgcat cttaaagggc
     7021 tcgaagttac agcagatttt tcagatggtg ttctcttgat cactgaacat aggctgacag
     7081 acgcttttct gctgttagcc ctagtcattc tgaccatgca cctgttaatt tcagaaagag
     7141 aagaaggtac actgcctttg gttaaagcta ctaaatacgg ctatggcagc accatctttg
     7201 ctaaggcggt cgttttgtta ttactaaccc tttttttggc actgatattt tatagtacta
     7261 atctaattgt tgccacaggc gaggtggggc ttggtgattt gaaccggccg attcaggccc
     7321 ttgctggtta ccgttccagc cctctaaaga taacagtgaa acaatatctg gggttgtttt
     7381 tttctgcaaa actacttggc gtcttagcca atctgtacat tttctttttg cttggcattt
     7441 tatgccgcaa tcatcttttt tcctgtctgc ttggcatcct tgttttaggt ctggaagctt
     7501 tgctttacaa aaatattgac ctgcattcat ggctgagccc tgtcaggcag tgtaatctaa
     7561 cagcactgct ggataccggc agctattttt ctaattacaa taatctcaac ctctttggtt
     7621 atcctgtaaa caccattaca gctggcgttg tcactggctt caccgcaatt attttcgggg
     7681 ttgtaatggg gatctttgtc tttagcaaag aagctgcttc tgatatgagg cgaaactggt
     7741 taaaagaaaa acttacagca aacataacat ttttcaaagg tctgcatttt aacttgttaa
     7801 ggcatgaggc ttataaactt ttgattatga ataaaggtct tttgctgata ctcatcttta
     7861 tcgtcagcca agttttctat tttaacggca tgatgtactt tatcgatcaa gaggaattct
     7921 actatcaaaa ctactctacc atgcttgcag gaggcttaac agaagaaaaa aacgcctttt
     7981 tacaaaatga gcaggaaaga atcgctgcaa gcgagcaaga acgagaactc ttataccaga
     8041 ggtacgagaa cggcgaaatt gatgaaaggt atctgtacta ctgcctttca cagttggagg
     8101 ttaatggttt taaaaaagat gcttttgaaa aagcacagac tcagtaccat cagttaatcg
     8161 ccatgcagga aaagaaacaa ccggtggctt atgtttatca aaccggatgg cataaattgt
     8221 ttgggcagga ggcaaaacgt cttgatttgc tcgaatacga taattttttc tgttcttgat
     8281 cttagctctt tcttcttatg aagctgttga gataatgact tttatgcatc aattaatttc
     8341 agtttccaaa aaaggcagga aagcagtgag aaatagaaaa attttaattt gtgtttgttt
     8401 tgccatgatt tctgctgcca tttctttttt gccaagaact atacgtgttt ttcaaaccta
     8461 tggggcaagc ggattaagtg cgccggcaaa aagcattatg gaaataccat gggcacctaa
     8521 tggactttct attgcaaact tttttattct ttaccaagta atgcgtttga tgatagcagc
     8581 actggcaggc gtgtttattc tcgtggcctc tcacaaaact ggcaatacaa taacaactat
     8641 cttgatttca acggcagtat taatattgcc tcttgctaca atgttgttag cataagtaat
     8701 tatacagcat ttctgtagaa tagccatatt tgtaggagaa cagcgattag ctagaagatt
     8761 agtatacagc ggcacaatat tttccaatat tccaagtctt cacacgctgt atatggttct
     8821 ttccattttt gtggtttata tcatttagaa gtaaaatggt tccttaaagc aaagtcggtg
     8881 gagtttaact taccaccgac ttttcatacc ttaaccccgc aaaccaaact tctttttctc
     8941 cagcgtcaat tgtgcaggta aaaaagcaga taaaagctcc tccatcaatc catctcactc
     9001 agaagataat ggaccagaca catacggagc taccaaaact ctttctgtct tctcttctat
     9061 catgcttctc atacttttac ctggatcacc aactgcaacc accgcccagt tggctagagc
     9121 cttttccatg tgtcttaaat atggt
//
LOCUS       DASRMS010000020         1504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_272234, whole genome shotgun sequence.
ACCESSION   DASRMS010000020 DASRMS010000000
VERSION     DASRMS010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272234"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..670
                     /locus_tag="VFC74_01060"
     CDS             248..670
                     /locus_tag="VFC74_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23960.1"
                     /translation="MYGQYNPYMGATPQMQQRLNYLQQQQQQMYQPTMQQPMPMALKG
                     RIVTGMDEAKAAQIDLDGTSTFFPCPAEGKIYEKSIDLNGLPVFRVYQLSNPQEQKQV
                     VYAERSCVDNLIQRVDKLEKQLGGMNHEPDANNGNVTE"
     gene            633..857
                     /locus_tag="VFC74_01065"
     CDS             633..857
                     /locus_tag="VFC74_01065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23961.1"
                     /translation="MNPMQIMAMLQNSGNPMQMLTQMAQQNPMMSRAMQMGQGKNEVQ
                     LKETVRNLARQRGMSDEQFTQFLSQFGLKL"
     gene            906..>1504
                     /locus_tag="VFC74_01070"
     CDS             906..>1504
                     /locus_tag="VFC74_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23962.1"
                     /translation="MEGANIVPVMDMNRNNNYGDCWGGGMWFMWIIVLFALMGGWGGN
                     WNNRGNMGAEIFANGSMTRDQIADQFSMQDIKDGIRGVQNGLCDGFYAQNTTMLNGFN
                     GVQRDIMQTGYQLGNQLSENRFAQQQCCCETNRNIDAVRYENARNTCDIVNAVKEDGE
                     KTRAVLIANQIQDLRDKLADRDRDLQTANFQLSQQAQSAT"
BASE COUNT          463 a          289 c          379 g          373 t
ORIGIN      
        1 ccacggcagg tagaaatttg cgaactgaaa tttataaaag gtatgactaa ttatcaaatt
       61 gcagcggagc tgaatgtatc tgttaaaacg gtagacaagg aattgaatac tgcgtacaag
      121 cgaataacaa atgtattatc attcctttaa atgcaggagc cgccttttag ggcggttctt
      181 tttttgtggg gaatttatag ggattgtttt gctaaaaatc agctaaacta taagtgaggt
      241 gataagtatg tacggacaat ataaccctta tatgggagca acaccgcaga tgcagcaacg
      301 gctgaattat ttgcagcaac aacagcagca gatgtaccag ccaactatgc agcagcctat
      361 gcctatggca ttaaagggca gaattgttac cggcatggat gaagcaaagg cagctcaaat
      421 tgacctggac ggaacgagca ctttcttccc gtgcccggca gagggcaaga tttatgagaa
      481 gtctatcgac ctcaatgggt tgcctgtatt cagagtttat caattaagta atccgcagga
      541 gcagaaacaa gttgtatatg ctgaacgaag ttgtgttgat aatttgattc aacgtgtgga
      601 taagttggag aaacagctag gaggtatgaa ccatgaaccc gatgcaaata atggcaatgt
      661 tacagaatag tggcaatcca atgcagatgc ttacgcaaat ggcgcagcag aatcctatga
      721 tgagccgtgc tatgcaaatg gggcaaggca agaatgaagt gcagttaaaa gaaactgtac
      781 gtaaccttgc aaggcaacgc ggcatgagtg acgaacagtt tactcagttt ttaagtcaat
      841 tcggtttaaa gctctaatag cgcgcaatga gctttacata taattcctgg aggtgaaatt
      901 ttatcatgga aggtgcaaac attgttccgg taatggacat gaatcgaaac aacaactatg
      961 gtgactgctg gggcggcggt atgtggttta tgtggattat tgtcctgttc gctcttatgg
     1021 gcggctgggg cggtaattgg aataaccgcg gcaatatggg tgctgaaatt tttgcaaatg
     1081 gcagtatgac acgcgatcaa atcgcagacc aattttccat gcaggatatt aaagacggta
     1141 ttcgtggtgt tcagaatggc ttgtgcgatg gattctacgc tcaaaacact accatgctga
     1201 atgggtttaa cggtgttcag cgagacatta tgcagactgg ttatcagtta ggcaaccagc
     1261 tttccgaaaa tcgttttgct caacagcagt gctgctgcga aactaaccgc aacattgacg
     1321 cagtgcgcta tgagaacgcg cgtaatacct gcgatattgt taacgcagta aaagaggacg
     1381 gcgaaaagac cagagcagtt ctgattgcca accaaatcca agacctgcgc gacaagctgg
     1441 cagaccgaga ccgcgatttg cagaccgcta acttccaatt aagtcagcaa gcacagagcg
     1501 ctac
//
LOCUS       DASRMS010000021         1669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_943237, whole genome shotgun sequence.
ACCESSION   DASRMS010000021 DASRMS010000000
VERSION     DASRMS010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1669
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943237"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1591
                     /locus_tag="VFC74_01075"
     CDS             <1..1591
                     /locus_tag="VFC74_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HZK23963.1"
                     /translation="KQALLHPERIREISQYILNNFRQKTHRLHGNNKGFNAMFAVSSV
                     DAAKLYYETLNQLQKDSDKPLKIATIFSFAANEEQDAIGDIADESFEVSAMNSSAKEF
                     LNAAIADYNAFFKTSFSVDSNGFQNYYRDLAKRVKSQEVDLLIVVGMFLTGFDAPTLN
                     TLFVDKNLRYHGLMQAYSRTNRIYDATKTFGNIVTFRDLEKATIDAITLFGDKNTKNV
                     VLEKSYKEYMEGFTDIVTGEARRGFMEVVSELEQRFPDPAAIEKESDKKEFAKLFGEY
                     LRVENILQNYDEFASLKALQNVDMSDPDAVEAFKAEHYLDDEKLAELQTIRLPAERKV
                     QDYRSTYNDIRDWQRRQKAADAKDQSNIDWDDVVFEVDLLKSQEINLDYILELIFEHN
                     KKHKNKEDLVDEVRRVIRASLGNRAKESLVIDFINTTNLDDIGDKASVIEAFFEYAQA
                     EQQREAEQLINEESLNAEAARRYIKASLKREYASDAGTALNSVLPKMSPLNPQYLTKK
                     QSVFEKIASFVEKFKGVGGKV"
BASE COUNT          509 a          386 c          394 g          380 t
ORIGIN      
        1 caaacaagcc ttattgcacc ccgaacggat tcgcgaaata tcgcagtata ttctcaacaa
       61 ctttcgccag aaaacccacc gcctacacgg caacaataaa ggctttaatg ccatgtttgc
      121 cgtaagcagt gtggatgccg ccaagctgta ttatgaaacc ctgaaccaac tgcaaaaaga
      181 cagcgacaag ccgttaaaaa tcgccacgat attttcgttt gctgccaacg aagagcaaga
      241 cgccattggc gatattgccg atgaaagctt tgaagtgtcg gccatgaact caagcgccaa
      301 agagtttttg aatgcggcga ttgctgacta taacgcgttt ttcaaaacta gctttagcgt
      361 cgatagcaat ggctttcaaa actactaccg cgatttagcc aagcgggtca aatcgcaaga
      421 agttgacctg ctgattgtgg tgggcatgtt ccttaccggc tttgatgcgc ctacgttaaa
      481 caccttattt gtggataaaa acctgcgcta ccacggctta atgcaggcct actcgcgcac
      541 caaccgtata tacgatgcca ccaaaacctt tggcaatatc gtcaccttcc gcgatttaga
      601 aaaagccacc attgatgcca ttaccctgtt tggtgataaa aacaccaaaa acgtggtgtt
      661 ggaaaaaagc tataaagaat acatggaagg ctttaccgat attgttacgg gtgaagcacg
      721 gcgcggcttt atggaagtcg tcagcgaact ggaacagcgc ttcccagacc ctgccgccat
      781 agaaaaagaa agcgataaaa aagaattcgc caaactgttt ggcgagtatt tgcgggttga
      841 aaacattctg caaaactacg atgaattcgc cagcttgaag gcgctgcaaa acgtggacat
      901 gagtgacccc gacgcagtgg aagcctttaa agccgagcac tatttagacg atgaaaagct
      961 agcagagctg caaaccatcc gcttgcccgc cgagcgtaaa gttcaagact atcgctcaac
     1021 ctataacgat attcgtgact ggcagcgccg ccagaaagcc gctgatgcga aagaccaatc
     1081 caatattgac tgggatgatg tggtgtttga ggtcgacctg ctgaaatcgc aggagattaa
     1141 ccttgattac attctggaac tgatcttcga gcacaacaaa aagcacaaaa acaaagaaga
     1201 tttagtggat gaggtgcgcc gcgtaattcg tgccagctta ggtaaccgtg ccaaagaaag
     1261 cctagttatc gattttatta acacaaccaa tctggatgat attggcgaca aggccagtgt
     1321 gattgaagct ttctttgaat atgctcaagc agaacagcaa cgtgaagcgg agcagctgat
     1381 taacgaagaa agtcttaatg ccgaagccgc ccgtcgttat attaaggcct cacttaagcg
     1441 tgaatacgcc agtgatgcgg gtacagcact taactcggta ctgcccaaga tgagcccgct
     1501 caacccgcag tatttaacta aaaaacaaag tgtatttgaa aagattgcta gctttgttga
     1561 gaagttcaag ggggtgggtg gaaaagtata atgggtaatc ctctatttaa tggggctcag
     1621 aagtagagcc aggccgacat ggcccacttc tgccagggtg taaggccac
//
LOCUS       DASRMS010000022         1478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_4396, whole genome shotgun sequence.
ACCESSION   DASRMS010000022 DASRMS010000000
VERSION     DASRMS010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1478
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4396"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="VFC74_01080"
     CDS             <1..618
                     /locus_tag="VFC74_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23964.1"
                     /translation="VDSAKSTQIISAWRMAGVNSAESYRLGVEFKLGAVATAGKAIAD
                     AAKNAAGKISTYSTGQNFGQTFANGISSRWNQAYNAGYSLGLAANQGQRAALNIKSPS
                     RVAKENAENFGQSFVDNLLKMKDKAFAAGREFTQAALNPLEQPVNIHMTRSSSLSSIG
                     GRQSGGVEYGGGITQNITIQSPEPMSASDNARRIKQASRELALEW"
     gene            625..>1478
                     /locus_tag="VFC74_01085"
     CDS             625..>1478
                     /locus_tag="VFC74_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HZK23965.1"
                     /translation="MFYEIKYKNNAGEEVHFKAEKKGNLFVAPDEPSIPSSGNPYRLM
                     MFEGLGEVAAEMHLNKSFAQDGSTHSSTVLSERHPYLQFVLVGKDWEDLSRIRRQATR
                     VFSPKSKGTLEFSYGANSYVLKVIPESLPVYSQEDTSGKTQVASVSLVAPNPYWQNIA
                     ESKADISTETGNIEFPLEIPAEGLELSIRTISFITNIYNRGDVGTPIKVILKAAGTVV
                     NPFIENLDTGEFIRVKRTLVEGDTLEINTAFGNKRVEIIKPDGSRENVFHYIDYKSTF
                     FSLEGGDNT"
BASE COUNT          479 a          269 c          375 g          355 t
ORIGIN      
        1 gtagattctg caaagtcaac gcagataata tctgcatggc gtatggcagg tgttaattcg
       61 gccgaaagtt atcgcttagg tgttgaattt aagcttggtg ctgttgctac tgcaggcaag
      121 gctattgctg atgctgccaa gaatgcggca gggaaaataa gcacctatag cacaggtcag
      181 aactttggtc aaaccttcgc caacggtatt agttccaggt ggaatcaggc ttataatgct
      241 ggttattcac tagggttagc ggctaatcaa ggccagcgag ccgctttgaa cattaagtcg
      301 ccgtcaaggg tggcgaagga aaatgctgag aacttcgggc aatcgtttgt agataattta
      361 ctgaaaatga aagacaaagc ctttgcagca ggcagggaat tcacacaagc ggcattgaac
      421 ccgctagaac agcctgttaa tatacacatg acacgttcta gcagtttgtc gagtataggc
      481 ggcaggcagt cgggcggtgt ggagtatggc ggagggataa cgcaaaatat cacgatccaa
      541 tcgccagagc ctatgtcagc gtcagataat gcaaggcgta tcaagcaggc aagccgagag
      601 cttgctctag agtggtaggg gaaaatgttt tatgaaataa aatataagaa taatgcaggc
      661 gaggaagttc attttaaggc agaaaaaaaa ggcaatttat tcgtcgcacc tgatgagccg
      721 tcaattccgt caagcggcaa tccttatcgg ttgatgatgt ttgaggggct gggcgaggtg
      781 gcagctgaga tgcacctaaa caaatcgttt gcgcaggatg gtagcacaca ctcaagcaca
      841 gtcttaagcg aacgccaccc atatttgcag tttgtcctag tcggcaaaga ttgggaagat
      901 ttatcacgaa taaggcgaca ggcaacacga gttttcagtc ctaaaagcaa gggaacattg
      961 gaattcagct atggtgcaaa ttcatatgtc ttgaaagtta tccctgaaag tttgccagtg
     1021 tattcgcaag aagatacatc gggaaaaaca caggtggcgt cagtatcact ggtagcccct
     1081 aacccatact ggcagaatat agcagaaagc aaagcagaca tatcgacaga gactggtaat
     1141 atagaatttc ctcttgaaat accagcggaa ggattagaac taagcattag aacgattagc
     1201 tttataacta acatatacaa tcgcggggat gtaggtacac caataaaggt aatcttgaag
     1261 gctgctggta cagtggtaaa tccatttata gaaaaccttg atacaggtga atttataaga
     1321 gttaaaagaa cattagtcga aggagatacg cttgaaataa atacagcttt tggcaacaag
     1381 agagttgaga taataaagcc agatggaagc agagaaaatg tgtttcatta tattgattat
     1441 aaaagtacat ttttcagcct agaaggtgga gataacac
//
LOCUS       DASRMS010000023         2854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_205976, whole genome shotgun sequence.
ACCESSION   DASRMS010000023 DASRMS010000000
VERSION     DASRMS010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2854
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205976"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(136..258)
                     /locus_tag="VFC74_01090"
     CDS             complement(136..258)
                     /locus_tag="VFC74_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23966.1"
                     /translation="MPRTDGAKLTVPGVDLLTYWGLTVPDGTQRLTVPGVDLFV"
     gene            complement(333..2636)
                     /locus_tag="VFC74_01095"
     CDS             complement(333..2636)
                     /locus_tag="VFC74_01095"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK23967.1"
                     /translation="MKKKNGQLAALLLAIVMILSFSATPLFAAGDQTFKDVPESAWYH
                     GYVMMLYEKGIVKGYGETGEFRPGNKLTREQASKMVALAAGLAHEGLKANFPDVDKEG
                     WASPYIAALKDKGAIQGFPDGTFRPLANIQRGHAAKIVALAFGLREGDMVSALADLPQ
                     DVDVRTAIEMLESNGIVKGYSDTNLFKPEEEINRAEFSKILCIAMVVASIQKAEADPT
                     PENIAAAQALANQLPKKQDVPTQDYLQGRLDKVEEEEPPAGPVDPSPAEILAAALGGP
                     KFATVSGNRVTLIENVTITGTVTVPAGVTLNTGGKSVSGSSGSTLEVYGTVYASKFED
                     VAQFLQNGGSAKFHVGSSLQVYDSGNSRTDTFIGADTMSIDSGYVTISLNSTYGFDLT
                     ITDGSTATIKGHHMDGNLNGYRIAKADCYSVAGTLNINYRTEVSGKLEVKTNGKIHVT
                     SNGEIYIFDADSSGPGGSLTSAINSITGETDAKIINDSPAASTLNGHTLEKGKTYTWD
                     AATQNWSGIAVEGLVAALGAANVTVDGNIVTLKADVTINDILIIPAGVTLNTGGKAVS
                     GNGTVNVYGAVEAEAFTDVAGLVQAGHANFYAGSTLKIAGDTFIGENTMSVDEGFVTI
                     GLHAYTTTDTGFQLTIPSGSTATIKGYHMSGTNDDYRIAQKDKYLVQGVLNIAYNRVL
                     VTGLLEVEGSGKINIQSGSNVYLWGEGTHKKMISPVNTILGEAGAAIYNDLKGLSAGT
                     LNGIDIKEGLSKYTWDNSTSAWKETER"
BASE COUNT          698 a          699 c          567 g          890 t
ORIGIN      
        1 tttttcaatt ccaatccaat aatagtgaat gattgtatag tgtaaagatt tttgatagta
       61 ccaaacaaat gacgtttact ccattttgcg gcggttttac ttgcctacca cttaaacact
      121 tgcgcctcgc aacttttaaa caaacaagtc aacacctggc accgttaatc tttgtgtacc
      181 gtccggtact gtcaatcccc aataagtcaa caagtcaaca cctggcaccg tcagtttggc
      241 accgtcagtt cttggcaccg tcagtacctg gcaccgtcag taccttggca ccgttaaccc
      301 tccggtaccg ttaaccctcg tatcgttaat tctcatcttt ctgtttcttt ccaagcagat
      361 gtactattat cccatgtgta ttttgataat ccttctttga tgtcaatccc atttaacgtc
      421 ccagcagata accccttaag atcattgtaa attgcagctc ctgcttctcc tagtatagta
      481 tttacaggtg atatcatttt cttgtgggtt ccttcgcccc aaagatagac attagaacca
      541 gactggatat tgattttgcc agaaccttcc acttcaagaa gacctgtaac taacactctg
      601 ttgtaagcaa tatttaaaac tccctgcact aggtatttat ctttctgcgc aattctatag
      661 tcatcattgg taccagacat atggtaacct tttatggtag ctgttgaacc gctaggtatg
      721 gttagctgga atccagtatc tgtcgtagta tacgcatgaa gtcctatcgt tacaaagccc
      781 tcatctacac tcatggtatt ttcacctata aaagtatcac cagcaatttt taaggtactt
      841 cccgcataga aattagcatg tcccgcttgc actaatcctg caacatctgt aaatgcttcg
      901 gcttctacag ccccgtaaac atttacagtg ccattgccgc taacggcttt cccaccggtg
      961 tttaaggtta ctcctgctgg aatgatgagg atatcattta ttgtcacatc ggctttcaaa
     1021 gtaactatgt taccatccac tgtaacattc gcagcaccta aagctgctac aagtccttct
     1081 actgcgattc cactccagtt ttgagtagca gcatcccagg tataagtctt tcctttctct
     1141 aaggtatgtc cattcagggt actagcggct ggtgaatcat taataatctt tgcatcagtt
     1201 tcacctgtaa tactattaat agcagatgtt aaactgcccc ccgggccact actatccgcg
     1261 tcaaaaatgt atatttcccc atttgatgta acatggattt ttccgtttgt cttaacttcc
     1321 aacttaccac ttacttctgt tcgatagtta atgttgagag ttcctgctac tgagtagcaa
     1381 tctgctttag caattctgta gccattaaga tttccatcca tgtgatgtcc tttaatggtg
     1441 gccgttgatc cgtcagtgat ggttaggtca aatccataag ttgaatttaa acttatggtt
     1501 acataacctg agtctatact catggtatct gcacctatga atgtatctgt acggctgttg
     1561 ccagaatcat acacttgcaa ggagctgcct acgtggaact tagcagatcc tccattttgt
     1621 aagaattgcg caacatcttc aaatttacta gcgtatactg ttccataaac ttctaaggtg
     1681 ctacctgaac taccgctaac ggatttccca ccggtgttta gagttacccc tgctggaacg
     1741 gtgactgtac cagttattgt cacattttct ataagagtaa ctctgttccc actaactgta
     1801 gcaaatttgg ggccgcctaa agcagcagct aagatttctg caggactcgg atctaccggc
     1861 cctgccggcg gttcttcttc ttccactttg tccagcctgc cctggagata gtcctgggtg
     1921 gggacatctt gcttcttggg cagttgattg gccagggctt gggcggctgc aatgttttcc
     1981 ggggttgggt ctgcttctgc cttttggata gaggcaacta ccattgcaat gcagaggatc
     2041 ttggagaatt ctgctctgtt gatttcttct tccggtttga agaggttagt atcgctatag
     2101 cctttgacga taccgttact ttcaagcatt tctatagccg tcctgacatc gacgtcttgg
     2161 gggaggtcgg ccagggcgct gaccatgtcg ccttcccgga ggccgaaggc cagagctact
     2221 atcttggcag catggcccct ttggatatta gccaagggtc tgaaggtgcc atcggggaag
     2281 ccttggatag cacccttgtc ttttagggca gcaatgtagg gggaggccca tccctctttg
     2341 tcgacgtcgg ggaagtttgc cttgaggccc tcatgagcta aaccggctgc caaggcaacc
     2401 attttggatg cctgttcccg ggtcagttta ttcccgggcc taaattctcc ggtttcccca
     2461 taacctttta ctattccctt ttcatagagc atcatgacat agccgtgata ccaggcgctc
     2521 tcaggcacgt ccttgaaggt ctggtctcct gcagcgaata ggggtgtcgc cgagaaactc
     2581 agaatcatta cgattgccag caataaggcc gccagctgtc cattcttctt cttcattttc
     2641 gtcctccata tttaatattt ttgctaaact aaaaaaaagc tccggcagga taaactatat
     2701 tccctgccgg acccttggca attagaattg tcattaacag tgataaaaac ttgataacac
     2761 cttttttttc catgatgacc atctttcgct aattcatgtc ataaaagata aaacacaaat
     2821 tccggatagt ttcatttttg tgcaagtatt acag
//
LOCUS       DASRMS010000024         3755 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_206248, whole genome shotgun sequence.
ACCESSION   DASRMS010000024 DASRMS010000000
VERSION     DASRMS010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_206248"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VFC74_01100"
     CDS             <1..1187
                     /locus_tag="VFC74_01100"
                     /inference="COORDINATES: protein motif:HMM:NF021028.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HZK23968.1"
                     /translation="SDAQSVLNLTAEDDTKVMLYAVWVVNSYTVSYDANGGTGRTESS
                     SHSYDLAKTLMANGFKRTGYTFSGWAARSNGATIYKNEESVSNLTSENDETLTLYTVW
                     KGNSYTMNYDANGGMGNVASSRHTYGVAKALTANGFLRTGYVFTGWSTSPDGAVIYAD
                     EQDIMNITSVNGTTVTLYAVWKPVYWLSVVGGTGSGSYAATTRVSITADETPSGKVFD
                     RWISSNGGTFDAATHVSTTFTMPDQDVIVTATYRDAAILPTPSAPPLGQLKPASIQVD
                     EATGSMLISINIASLPEGTTAIKLPSGEIVSIDANASVLKLQLSQNEWEKYEQLVLVA
                     LNGEHIPSSSYVIDLSEEARQVGPEDSDGFFSILVWIVVSVLVIGGAMTAILIFLNKK
                     KK"
     gene            1411..1758
                     /locus_tag="VFC74_01105"
     CDS             1411..1758
                     /locus_tag="VFC74_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23969.1"
                     /translation="MQGNSLKGRVAALVVGFVVGFFIVLNSIFSDVFTFKERLYSFLL
                     VILFYGVIGLVFSLVSPSWKWGLWLAAPAVLLVIFYSISDGFRPLLYISYIVLTIVCA
                     CGGAYFGAKIRKK"
     gene            2019..2963
                     /locus_tag="VFC74_01110"
     CDS             2019..2963
                     /locus_tag="VFC74_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK23970.1"
                     /translation="MKKILQKTGRLLLILLCILFMFLLATTIFHHISLVYEAKKIVPN
                     GMLVEVDGHNIHIYAEGEKRNRPTLVFMSGSATVAPVYDFKSLYSMLSAEYRIVVVEK
                     AGYGYAEICEVERDIDTMLNEVRQGLTLAGESGPYVLFPHSMSGLEAVYWAQQYPEEV
                     KAIIGLDMAVPESYEYFDFSSVKQMMYLGRASVWLGLHRIPGVYSLDAAELTEDEIAQ
                     QKLLMYRNAVNIDYVLEGKAVYNNAVQVKSGGALNLAILMFVSNGGEIGEYWIPCQKR
                     FAAQNDAQLIHLDCGHYVHNFEHQYIAEKVLAYLTALQ"
     gene            3235..3495
                     /locus_tag="VFC74_01115"
     CDS             3235..3495
                     /locus_tag="VFC74_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HZK23971.1"
                     /translation="MEMLKVATKSSPNSVAGALAGVIRERGTAEIQAIGAGAINQAIK
                     AVAIARGFVAPSGIDLVCIPAFVDVMIGGEERTAIKLIVGPR"
BASE COUNT         1056 a          717 c          932 g         1050 t
ORIGIN      
        1 actcggacgc acagagcgta ttaaacctca cagcggaaga cgatacgaaa gtgatgctgt
       61 acgctgtctg ggtggtcaac tcttacacgg tcagttacga tgccaatggc ggcacgggca
      121 ggaccgagtc gagcagccac agctatgatc ttgcaaaaac actgatggct aacgggttca
      181 agcgtacagg ctacaccttt tcgggttggg cagcccgctc aaatggtgca actatctaca
      241 aaaatgaaga gagtgtgtct aacctcacgt ctgagaacga cgaaacgttg acgctgtaca
      301 ctgtgtggaa gggcaactcc tacaccatga attacgacgc caacggtggc atgggcaacg
      361 ttgcctcaag ccgccatact tatggtgtag caaaagcgct gacagctaac ggctttttac
      421 ggacgggcta tgtttttacg ggttggtcaa ctagtccgga cggtgcggtc atctatgcag
      481 acgaacaaga tataatgaac atcacatctg taaatggcac aacggtgacg ctgtacgcgg
      541 tttggaagcc cgtgtattgg ctgtccgtag tcggtggtac gggtagcggc tcatacgcag
      601 cgactacacg ggtatctata acagcagatg aaacgccgtc gggcaaggtg ttcgacaggt
      661 ggataagtag taatggcggc acctttgatg ctgccactca tgttagtact acgtttacca
      721 tgccagatca agacgtgata gtgacagcta catataggga tgcggctatt ctaccaacgc
      781 catcagcccc cccgcttggg caactaaagc cggcttccat tcaggttgac gaagccacgg
      841 ggagtatgct gatcagtatc aacatagcaa gtttacccga aggcacaacg gccattaagt
      901 tgccgtccgg cgaaattgtc tccattgatg ccaatgcaag cgtattaaag ctacaactca
      961 gccaaaacga gtgggagaaa tacgagcagc tagttcttgt tgcgctaaat ggtgaacaca
     1021 ttccctcaag cagttatgta attgatttat cagaggaggc caggcaggtt ggcccagaag
     1081 atagcgatgg attcttttct atccttgttt ggatcgttgt gagcgtttta gttattggcg
     1141 gagcgatgac agcaatactc atttttctga acaagaagaa gaaatgatga attgtgaggt
     1201 tggttgacat ctgtatctgt tacagtgctt aaggcagtag tatctttctt tgcgctgtcg
     1261 acggcgacag gcaaactgat aaagacttta cacagaggca tcttgatagg gtttgccagc
     1321 gtggtaaacc cttgtaatta aatattcatt ccctttaacg caaaaaagca gtatgataca
     1381 agtacagagt tattatgtgg agggatgtat atgcagggta attccctgaa aggacgggtg
     1441 gctgctttag tagtcggttt cgtggttggt ttctttatcg tcctcaattc aatcttttct
     1501 gatgttttta cttttaagga gcgtttgtat tcctttttac tggtgatttt attttatggc
     1561 gtcattggct tggttttcag cctggtatca ccatcctgga aatggggctt gtggctagct
     1621 gccccggctg ttttgctggt tatcttctac tctatctccg acggttttcg cccacttttg
     1681 tatatttcct acatcgttct cacaatagtc tgcgcctgtg gcggggcata ctttggtgcc
     1741 aaaatacgta aaaaatgaag gatgtcaaat caagatccat atacttaaaa aaccctgtaa
     1801 gctaaaaccc tcatatcact aatgaatgag ggttttatta tagtagcgta gtgcttgata
     1861 aaataatgtc taacttagca aaaatggaac agagtgaagg ggaggatgtt gggggatgaa
     1921 atagcccctt cctgcaggta ttgtaagctt gatcaaggaa gagttgttta tgaactgcat
     1981 taacttggag ttttaagtaa tcaactacag gaggagaaat gaaaaagata ctgcaaaaaa
     2041 cgggcaggct gctattgata ttactttgta tcctttttat gtttttgtta gcaacaacta
     2101 tttttcatca tatttccctt gtttacgagg cgaagaaaat cgtccctaac ggcatgcttg
     2161 tggaagttga cggtcataac atacatatat atgccgaggg agaaaaacgc aataggccca
     2221 ctttggtttt tatgtccggt tccgcaaccg ttgcaccagt ctacgacttc aagtccttat
     2281 acagtatgct ctcggctgaa tacagaatag tcgtggttga aaaagcgggc tacggctatg
     2341 ctgagatctg tgaggtagaa cgggatattg acactatgct taacgaggtc aggcaggggt
     2401 tgacacttgc aggagaaagc gggccatatg tactgtttcc gcattccatg tctggtttgg
     2461 aagctgtata ttgggcgcag caatatcctg aggaagtaaa ggccattatc gggcttgata
     2521 tggctgtgcc ggaaagttat gagtacttcg atttttcaag tgtcaagcaa atgatgtatc
     2581 tcggacgtgc gtctgtttgg ctggggctgc acagaatacc tggtgtatac tctcttgatg
     2641 ctgctgaact cactgaagat gaaattgcac agcaaaagct gttgatgtac agaaatgctg
     2701 taaatatcga ctatgtgttg gaagggaagg cggtatacaa caatgctgtg caggtcaaat
     2761 caggcggtgc tctaaattta gcgatactta tgtttgtatc taacggagga gaaatcggag
     2821 aatactggat accgtgtcaa aagagatttg ccgctcagaa cgatgcgcag ctaatacatc
     2881 ttgattgcgg acactatgtt cataattttg aacatcaata catcgcggaa aaagtcttgg
     2941 cgtatcttac agcattacaa taaagtatct tgagggaagg taaacttgct tatttagtaa
     3001 tattacagcg aaagttaata aaagcattat catcatcata gctgcggcaa tatgccgcag
     3061 cttttaaatt accaattatg cagaaggcgc agtcgggggc atttatttta caaatatttt
     3121 aagcgaataa aggaattaag gaatttatga agaactaata cttaaccagt tagaatattc
     3181 tgaaatatag ggctaatatt taggaatgtc tcaaaaaagg agctgctagc attaatggaa
     3241 atgttaaaag tcgcaacaaa atctagtccg aattctgtag ctggagcttt ggcaggtgtc
     3301 attagagaaa gaggaacagc cgaaatccag gcgattggcg caggagctat taatcaggca
     3361 attaaagcgg tagctattgc cagaggattt gttgctccca gtggtattga ccttgtctgc
     3421 atccctgctt ttgtagatgt gatgattggt ggggaagaga ggactgcaat caaactaatt
     3481 gtagggcctc gctgatattc gagtccgttg agtggagaag aaatatagga aggaccggga
     3541 ttattcccgg tcacttccgc tggttcaacg cagctaaact aaggcaaaag ccttcacaaa
     3601 agttcttctg ttgtgaggat ttatggttat gatttgctat tttgtacgct gcgtgttata
     3661 atgaagcata atcttgtttt ttcaagtgct tgattccgat tttgaaatac caaaagatac
     3721 tggaattgtt aggcaggaaa ctcataatta tgggt
//
LOCUS       DASRMS010000025         1249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_676258, whole genome shotgun sequence.
ACCESSION   DASRMS010000025 DASRMS010000000
VERSION     DASRMS010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1249
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676258"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1249
                     /locus_tag="VFC74_01120"
     CDS             <1..>1249
                     /locus_tag="VFC74_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HZK23972.1"
                     /translation="KISTLEDALKNASESFGENDRRTQNWAIQLNNAKAQLNGMEREL
                     DETAGSADDLSEELEDTGKSAEEAGGKFESFGGVLKGIGVAMGTVAIAAGAAAFKLGK
                     EVLQQFGELEQNLGGSEAVFGAYAASIQKTGEEAYKNMGVSQSQYLATANKMGALFQG
                     SGIEQQKSLELTERAMQRAADMASVMGIDMQMALDSVAGAAKGNFTMMDNLGVAMNAT
                     NIEAYALAKGLNFTWASATQAEKAEVAMQMFFENTQQYAGNFAKESTETITGSIGLLQ
                     AALGSFTAGLGNANADMTNLTENLVEAFQAVVTNFVPVIENIVAALPMAMDAILSAIG
                     DLLPTLLETVTTLFTQVLETLLSLLPELIPAAVDAVMTIVGALIENLPLLIEAAVELV
                     SALVEGIGQALPELIPAAIEAVIT"
BASE COUNT          328 a          283 c          341 g          297 t
ORIGIN      
        1 taaaatatcc acccttgagg atgctttgaa gaacgcctcg gagagttttg gcgagaacga
       61 ccgccgcact cagaactggg ctatccagct taataatgcc aaggctcagc tcaatggcat
      121 ggagcgtgag cttgatgaaa ccgcgggcag cgcagacgat cttagtgaag aactggagga
      181 taccggtaaa agcgctgagg aggccggggg caaattcgag agcttcggtg gtgtccttaa
      241 aggaataggc gtagcgatgg gtactgttgc tatagccgca ggagccgccg cctttaagct
      301 gggaaaagaa gtcctgcagc agtttggtga actagagcaa aacctgggcg gctcggaggc
      361 agtatttggc gcttacgccg cttccattca gaaaaccggt gaggaagcct ataaaaatat
      421 gggcgtttcc caaagtcagt atcttgccac cgccaataaa atgggcgcat tgttccaagg
      481 ctccggcatc gagcagcaaa aaagccttga acttacggag cgggctatgc aaagagcggc
      541 tgatatggcg tctgtcatgg gtattgatat gcagatggca cttgattctg tagccggtgc
      601 agccaaaggc aatttcacca tgatggataa cttaggggtg gccatgaatg ctaccaacat
      661 tgaagcatac gctttagcca agggactaaa ttttacctgg gcaagcgcaa cccaagcgga
      721 aaaggctgaa gttgctatgc agatgttttt tgagaataca cagcagtatg ccggaaactt
      781 tgccaaagaa tctactgaaa ctattactgg ctctatcggt ctgttgcaag ctgcacttgg
      841 ctcctttacc gcaggacttg gcaatgccaa tgccgatatg actaacctta cagaaaacct
      901 ggtagaagct tttcaggcgg tagttacaaa ctttgtgcct gtgattgaaa atatcgtggc
      961 tgctttgcct atggctatgg atgcaatttt atccgctatt ggagatttac tccccaccct
     1021 gcttgaaacg gtaacgaccc tatttacaca agtgctggaa accctcctga gtcttttgcc
     1081 ggaactaata ccggcggcgg ttgatgcggt gatgaccatt gtaggggcac tgattgaaaa
     1141 cctgcccctt ttgattgagg cggcagtgga actggtatcg gctcttgtag agggcatcgg
     1201 tcaggccttg ccggagctta ttcctgctgc aatagaagca gtaataacc
//
LOCUS       DASRMS010000026         1096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_408441, whole genome shotgun sequence.
ACCESSION   DASRMS010000026 DASRMS010000000
VERSION     DASRMS010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1096
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408441"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            286..543
                     /locus_tag="VFC74_01125"
     CDS             286..543
                     /locus_tag="VFC74_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23973.1"
                     /translation="MHGFQLRFDLTPDNMLEITPDGDMTPEEGVKFAEDYSLALVKQG
                     KPAEAAEALGRDVEIIKSYRKRRSVNFSDWLVPVIIIDKEV"
     gene            complement(627..992)
                     /locus_tag="VFC74_01130"
     CDS             complement(627..992)
                     /locus_tag="VFC74_01130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23974.1"
                     /translation="MNLGINARLAAFYLKQLGYALAGKPKVQAVFDLVIEQGFYPTQN
                     KYMCNAIIAAYKEGHLPRKAAAEALREVEAYVESLEPTKVYACTLFAVYREKNLSYCP
                     EVQGVEIFSDWKNRPLAIA"
BASE COUNT          305 a          271 c          216 g          304 t
ORIGIN      
        1 ttccttgact tatttccttt actgccacat agtattgggt ttccttaaaa ttactgcttt
       61 atttccttcc tatccttcta aaaatttcca cctaagaaaa ttattgacac ctgccaaatc
      121 ctcctataac ctgctgacaa atctcacaga aaaaggaacg aaactatgtt tgcaattact
      181 cttcaggctg ctcttataca ttactgtaag cgactaaaag tagaccctga cctaatcacc
      241 accctatctg cacctcctcg ctcacgaata gcagccataa atatcatgca cgggtttcag
      301 ttaaggtttg acttaacgcc tgacaacatg ctggaaataa cacctgatgg cgatatgaca
      361 cctgaagaag gcgtaaaatt cgctgaggac tactccctag ctctagtcaa acagggtaaa
      421 ccggctgaag cagctgaagc tttaggaaga gacgtggaaa taatcaaaag ctaccgtaag
      481 cgtaggtctg tcaatttctc tgactggctg gtccctgtga taattatcga caaggaggtt
      541 taacacaacc tccccctgct gccccaaaaa aggctctcct aggacgcccc gaaagttaca
      601 gaggcgcttc tgtgggggtc agttacttaa gctatagcaa ggggtcgatt cttccaatcg
      661 ctgaagattt cgactccttg tacttcagga caataagaca ggtttttctc tctatacacg
      721 gcaaacaagg tgcaggcata cacttttgta ggttctagag actccacgta agcctctacc
      781 tcccttagcg cctcagccgc tgcctttcgg ggcaagtgac cttctttata cgctgcaatt
      841 atcgcattgc acatatactt gttttgggta gggtaaaaac cctgctcaat aactaagtca
      901 aatacggcct gcaccttagg tttgcctgcc aaagcgtagc ccagttgttt tagataaaac
      961 gctgccagtc ttgcattaat accaagattc atttgaaatt tcctcgatag aagggcggtt
     1021 ggcccagttg ttgaaaatag ctacaccttg tattttgggg tcatactcga cctccagtat
     1081 gttaaaaagg tcggct
//
LOCUS       DASRMS010000027         1168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_207569, whole genome shotgun sequence.
ACCESSION   DASRMS010000027 DASRMS010000000
VERSION     DASRMS010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1168
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207569"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="VFC74_01135"
     CDS             <1..1101
                     /locus_tag="VFC74_01135"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HZK23975.1"
                     /translation="QSRLLFLSRSFPDWIEGYTMEQWFASLRSVFFSQTIAIFIGLTL
                     FPIILFLVRRTSILEKLAVFCFVLTPLLYLVTLVAGKGGETHGAGLWINLPGGFSLQL
                     TEFAKITWIIVLANFFKTRANRRNIIIFALWAAFNAGLVLLLPDLGSLMVLLPTTLIV
                     FAIMTSEYLLSFLALTGASVVGVLAYNAMPYVQRRLYGWFSLWDHLNDDNRQIVYGLQ
                     AVGRGGLFGRGVGNGTPEGIPLASSDMIFSILWEEMGLLIAVSVIVLFFVIWLRSASA
                     IITARDGFTSSLILGLSTCLLMEALIVIGGTTGLLPLTGVTLPFIARGGSSIIAKWIM
                     TVLLAGMVARSRRLSISKKAQNSIRKKREATL"
BASE COUNT          271 a          228 c          279 g          390 t
ORIGIN      
        1 cagtcgcggc tactgttttt atcccgtagt tttccggact ggattgaagg ctacacgatg
       61 gaacaatggt ttgcgagttt aagatcggtg ttttttagtc agaccatagc aatttttatc
      121 ggtttaactc tttttcccat catcttattt ttggtcagac gcacaagtat tttagaaaaa
      181 ctggcggtat tttgctttgt gctgacaccg ctgctttatc tggttacttt agtagcaggc
      241 aaaggcgggg aaacacatgg agcgggtttg tggattaatc tgccgggtgg gttttctttg
      301 caattaaccg agtttgctaa aataacctgg attatagtac tggctaactt ttttaaaacc
      361 cgggcaaaca gacgcaacat tataatcttt gctctttggg ctgcgtttaa cgccggttta
      421 gtcttgctgc tgcctgatct gggttctctt atggtgcttc ttcccacaac tttaattgtc
      481 tttgccatta tgacctcaga atatctgttg tcatttctgg cactgacagg agcatcagta
      541 gttggcgttt tagcttataa tgcaatgcct tatgtgcagc gacgtttata cggctggttc
      601 tcattatggg atcacctaaa tgacgataac cgccaaattg tatatggatt acaggcggta
      661 gggcgcggtg gactatttgg ccgcggtgtg ggaaatggca ctccggaggg catccctctg
      721 gcgagttctg atatgatatt ttccattctc tgggaggaaa tgggtttact tatagcagtc
      781 tctgtcattg ttctcttttt tgtaatctgg ttgcgatcag catcagcgat tatcacagcc
      841 cgtgatgggt tcacttccag cttgatttta ggcctcagta catgtctctt gatggaagcg
      901 ttaattgtta ttggaggtac caccgggctt ttgcctctga ccggagttac tttgcctttt
      961 attgcgcggg gcggaagttc gatcatagcc aaatggataa tgacagttct tctggcagga
     1021 atggttgcac gatcgcgccg cttatcaatt agcaaaaaag cacaaaacag tatcagaaaa
     1081 aagcgtgagg caactctatg aagcagttac gatttaatat caaaatattt ttgctgatat
     1141 ttattggtat gtttattctg tttacggt
//
LOCUS       DASRMS010000028         1142 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_274906, whole genome shotgun sequence.
ACCESSION   DASRMS010000028 DASRMS010000000
VERSION     DASRMS010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1142)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1142)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1142
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274906"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..294
                     /locus_tag="VFC74_01140"
     tRNA            224..294
                     /locus_tag="VFC74_01140"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:256..258,aa:Gln,seq:ttg)
     gene            318..420
                     /locus_tag="VFC74_01145"
                     /pseudo
     tRNA            join(318..351,386..420)
                     /locus_tag="VFC74_01145"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /pseudo
                     /anticodon=(pos:349..351,aa:Phe,seq:aaa)
     gene            495..566
                     /locus_tag="VFC74_01150"
     tRNA            495..566
                     /locus_tag="VFC74_01150"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:528..530,aa:Thr,seq:tgt)
     gene            660..1139
                     /locus_tag="VFC74_01155"
     CDS             660..1139
                     /locus_tag="VFC74_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23976.1"
                     /translation="MAKPLRPTNTIASSDPFGLRWRKRINTTPCTIVIRYNENGAVIK
                     ITENKTKKTIIKMVRRYYKDEPDIRAGINAALDKLFAHLEDGYTGKVVCVSSDFQNTF
                     TPGKVYHMQDGIINCNDGFYNPEGPRYKTLDDLNTIGGHYFIPYVGENEIGVKRCTE"
BASE COUNT          360 a          270 c          252 g          260 t
ORIGIN      
        1 tggtcgagtg tgcagacctg cggaataccg tcatacaaaa acgcgcacga acaagacagc
       61 gccacctctg ccacagcagc aagtgccccg cgcctgtcgt ctgtaaagcg gaaatcatcg
      121 cttatagaat agccagacgg aatgaccgcc cgcgaagctg ttcaacttct gttaatgctg
      181 gattgcaacc aagagtttgg tcaaccccgc gttgcaatca cattggggta tcgtcaagcg
      241 gtaagacaca gcactttgac tgctgcatca caggttcgaa tcctgctacc ctagttctcc
      301 gtgtttatgg agtgcatgac catgtagctc agttggtaga gcaatcaaaa aaacggatgt
      361 tgacaaacat ccggacagct aaaacatgaa gtgtcacagg ttcaaatcct gtcgtggtca
      421 aacaagtaaa taaggacgca gacagcaaac ttttaaattc tttctgcaaa aaagatataa
      481 tcgcgtcctg taacgcacgt gtagtctaat tggtaaaaca catgccttgt aagcatgaga
      541 tttgggttcg actcccaaca ctggctttta ttttttacga aaggagtatt agacaattgc
      601 ctaattagca ttagacatcc accaaaatct aaattcaata aactacataa ggaggagtta
      661 tggcaaaacc gctcagacct accaacacaa tcgcctcgtc ggatcctttt ggattgagat
      721 ggcggaagcg catcaatacc actccgtgta ccatcgtaat tcgctataat gaaaacggcg
      781 cagtaatcaa aatcacggaa aacaaaacaa aaaagaccat cattaagatg gtgcgccgtt
      841 attacaaaga cgagccggac attagagcag gaatcaacgc cgccctcgac aagctgttcg
      901 cgcatttaga agatggatat accggcaaag tcgtctgcgt gtcgtcggat tttcagaaca
      961 cgttcacgcc gggcaaggtc taccacatgc aggacggtat catcaactgc aacgacggat
     1021 tttataatcc ggagggtccc cgctacaaaa cacttgatga tttaaacacc atcggcgggc
     1081 attattttat cccctacgtg ggtgaaaacg aaattggagt taaaagatgc acagagtaaa
     1141 aa
//
LOCUS       DASRMS010000029         2130 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_141707, whole genome shotgun sequence.
ACCESSION   DASRMS010000029 DASRMS010000000
VERSION     DASRMS010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2130)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2130)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2130
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141707"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            221..742
                     /locus_tag="VFC74_01160"
     CDS             221..742
                     /locus_tag="VFC74_01160"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009171585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK23977.1"
                     /translation="MSCTIRSATYEDLRDILAIYNHAILNTTAVYHYHPHTYTERLAW
                     YEQKFAAGLPVLVAIAEEQVMGFATYGPFRPWPAYKYTIEHSLYVHPEHQHKGVGSLL
                     LKEIIRVAGENEYKTLIAGIDSSNIQSMMLHKKNGFQQVGVISKAGYKFDHWLDLVFY
                     QLELPGPQKPTIE"
     gene            772..1308
                     /locus_tag="VFC74_01165"
     CDS             772..1308
                     /locus_tag="VFC74_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase ligase N-terminal
                     domain-containing protein"
                     /protein_id="HZK23978.1"
                     /translation="MTSDKSLLFVVQQHHAMFMHWDFRLEMDGVLVSWSIPDGPCTEP
                     QIERLTFRVEDHVYGYKDFEGVIPGNDFSSGKVIVWDQGEYKPHNLEAVVQLHETWLA
                     AGKLEFTLYGEKLQGRWSLTRTPEKLYGEETWFLSKGKDQYACSGEIVKECPKSVVSG
                     KTVDEVSVKDGIFNLNNY"
     gene            1855..>2130
                     /locus_tag="VFC74_01170"
     rRNA            1855..>2130
                     /locus_tag="VFC74_01170"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          624 a          405 c          499 g          602 t
ORIGIN      
        1 catgtgttga actattttca taagacttca cctcgagtga ctatttttag ttacttgtcc
       61 ttccgggacg gtgaagtctt ttttctacaa attgggggtg ttttcctcca aaagccctat
      121 agtaagttta cactaacgta aaaggttgac cttcgctgtt aggcatacta tgattaaaag
      181 tgtcattaag agggacctag ctgtcagggg gggatagggt atgtcctgta caatacggtc
      241 ggcaacatat gaagatctgc gggatatttt agctatctac aatcacgcta ttcttaacac
      301 tacggctgtc tatcattatc acccacatac atatacagag cgtttagcat ggtatgagca
      361 aaaatttgca gccggactcc ctgtgcttgt ggctatagca gaagagcaag tgatgggttt
      421 tgcaacatat ggcccgttta gaccttggcc ggcttacaaa tatactattg aacattctct
      481 ctatgtgcat ccagagcatc aacataaagg cgttggctcg ttactattaa aagaaattat
      541 ccgtgtggcc ggtgaaaatg aatataaaac gctgattgca gggattgatt cctcaaacat
      601 acagagtatg atgctgcaca aaaaaaatgg ttttcagcaa gtcggggtca taagtaaggc
      661 gggctataaa tttgaccatt ggctcgattt agttttttat cagttagaac ttcccggccc
      721 ccagaaacct actattgagt aataaaatat aaaaaaaagt gagcgatttt gatgactagt
      781 gataaatctt tactatttgt agtgcagcag caccatgcaa tgtttatgca ttgggatttt
      841 cgtttggaga tggatggagt gcttgtttcc tggtctattc ctgatgggcc ttgcacagag
      901 ccacagatag aacgtttgac atttcgtgtt gaagatcatg tttatggcta taaagatttt
      961 gaaggagtta tccctggtaa tgactttagc tctggtaaag tcattgtttg ggatcaggga
     1021 gagtacaagc ctcacaatct cgaggcggtg gtccagctgc atgagacatg gcttgctgct
     1081 ggtaagctag aattcactct ttatggagaa aaactacagg gtcgctggag cttgacgcgt
     1141 acaccggaaa agctatatgg tgaggaaacg tggtttttaa gtaaaggaaa agaccaatat
     1201 gcgtgcagcg gagaaattgt caaagaatgc ccgaaatcag tagtgtcagg aaaaacggtt
     1261 gatgaagtta gtgttaaaga cgggattttt aacctaaata actattagaa gttcaccaaa
     1321 ttcgggtttg ctaaccggtt gacaaatttc gcaagtcatg ttactatatt ttttgtgctg
     1381 gttattggtt gcggtaaact ttttagatca gtttttcttt tgccaagatg tacttgctgt
     1441 tttaatgaaa gaatactggt acagtaactt tcccgcacta atcatcttac atacttgctg
     1501 ccacgcaaga aacggaattt gggcgaccgc cttacatttt accaccaatt ggatgttgac
     1561 ataacatcgt agaaatggta agatagaatt ccggtcgcga gagcggcaag gatgtaacac
     1621 attaaaatag caagtctttc agcaagcgca cagcgcagct gcaagacagc ctaaagctct
     1681 ttgaaaactg aacaggaaat atgtaacgcc atgcgaagta gacatcgaag agatgttaaa
     1741 attttacgtt tcatcaaggc agagcctaac agctccgaac cggatgagca actttggttt
     1801 caaataagag ccaagaacaa tctgtaaacg agaagcttaa tcgcttttca ctatattacg
     1861 gagagtttga tcctggctca ggacgaacgc tggcggcgtg cttaacacat gcaagtcgaa
     1921 cggagttact ctctagcttg ctagagagta atttagtggc gcacgggtga gtaacacgtg
     1981 ggcaacctgc ccggaagtct gggataacac cccgaaagag gtgctaatac cggatatctt
     2041 taggaactcc catgagtttc taaagaaagt ttttcgcttc cggatgggcc cgcgtcccat
     2101 tagctagttg gtgaggtaac ggctcaccaa
//
LOCUS       DASRMS010000030         1033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_745683, whole genome shotgun sequence.
ACCESSION   DASRMS010000030 DASRMS010000000
VERSION     DASRMS010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_745683"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1033
                     /locus_tag="VFC74_01175"
     CDS             <1..>1033
                     /locus_tag="VFC74_01175"
                     /inference="COORDINATES: protein motif:HMM:NF012688.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HZK23979.1"
                     /translation="ALQREVTSIAKDELPQLIAEADLVAESALIKEVIALRQQTGMTS
                     VKKYQRMIECAGDDGRAHGLFQFYGANRTGRWAGRLIQLQNLPRNYIDQIANARNLVR
                     KGAFNTINILYDNVTDILSQLIRTALIAPEGKLLVAADFSAIEARVLSWLAGEEWRMQ
                     VFATHGKIYEASAAMMYNLPIESIAKDSLERHKGKVAELALGYQGAAGAMKQMGAEAM
                     GLDDAEIGNIVRVWRGKNPKIVGLWAAVDNAVKEAVQKSCEIVLTAYRNITINSEGKV
                     LTILLPSGRKLFYQKPRMTRNKWGQPSVKYMGQHHSRWVWIESYGGKFVENICQAIAR
                     DILGVAMLKM"
BASE COUNT          333 a          193 c          269 g          238 t
ORIGIN      
        1 cgcactgcaa agagaagtta catcaattgc aaaagacgaa ctaccacagc taatagcgga
       61 agcagacttg gtggctgagt cggctttaat taaagaagtt attgcgctaa gacaacaaac
      121 tggcatgacg tcggttaaga aatatcagcg catgattgag tgtgcaggag atgatggccg
      181 ggcacatggc ttgttccaat tctatggagc gaaccgcacc gggcgttggg ctgggcgctt
      241 aatacaactg caaaatttac ctagaaacta cattgaccag atagcaaatg ccagaaacct
      301 agtacgtaag ggtgctttca acacaattaa cattctctat gataacgtaa cagatattct
      361 atcacaattg ataagaacgg cgttaattgc gcctgagggt aaactcttag tagccgctga
      421 ctttagtgct attgaggcaa gagttttatc atggttagca ggtgaagagt ggcgaatgca
      481 ggtatttgca acgcacggca agatatatga agcgtccgcc gcgatgatgt acaatttacc
      541 aatagagtct attgctaaag atagcttgga aagacataaa ggaaaagttg cagaattggc
      601 attaggttac caaggagcag cgggtgcgat gaagcagatg ggtgccgaag cgatgggttt
      661 agatgatgct gaaattggaa atatcgtaag agtatggcgc ggcaagaacc ctaaaattgt
      721 agggctatgg gccgcggtag acaacgccgt aaaggaagca gttcaaaaat cttgcgagat
      781 agtgctcaca gcatatagaa atataactat caactcagag ggcaaagtac taacaatttt
      841 gctaccttca ggccgtaaac ttttctacca aaagccgcgc atgactagaa acaaatgggg
      901 gcaaccctct gtaaagtaca tggggcaaca ccactcgcgc tgggtgtgga tagaatcata
      961 tggtggaaag tttgtagaga atatatgtca agcaatagcg cgggacattc taggggtggc
     1021 catgcttaaa atg
//
LOCUS       DASRMS010000031        21845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_814330, whole genome shotgun sequence.
ACCESSION   DASRMS010000031 DASRMS010000000
VERSION     DASRMS010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 21845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21845
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814330"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..900)
                     /gene="aroD"
                     /locus_tag="VFC74_01180"
     CDS             complement(145..900)
                     /gene="aroD"
                     /locus_tag="VFC74_01180"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF013640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HZK23980.1"
                     /translation="MQSKKELHVKGRIIGGSNTNICVPLLSPTLPELLAEAEAMARLQ
                     PDLVEWRVDYFRAAKNLAAVTAALFKLRTVLPACPLIVTCRDHVEGGCQELSQPFRMA
                     LLQTLLQTGQTDFIDVEYSLGQDKLVPLLTAAQQQGAHVIVSYHNFAQTPAPSALFAK
                     LLQLQKTGADIIKIAVMPQEPQDVLNLLTATLTFATDAAQVPVVAISMSALGTISRLA
                     GSTFGSAITFASGQAASAPGQLPFAQLKSLLKQ"
     gene            complement(1080..1895)
                     /locus_tag="VFC74_01185"
     CDS             complement(1080..1895)
                     /locus_tag="VFC74_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HZK23981.1"
                     /translation="MVAYLSQFNIIWVGVLASLAAGLATGVGCLPIFFVKEVPKKVLD
                     ALLGAAAGVMLAATSFSLIIPAIEAGGQGIKGASIAIVGILAGGIFLDVVDTFFPNTN
                     LQVGPVPETKKHSSKLLTMSPSLRRVWIFVLAITIHNFPEGMAVGVGFGDGQIANGLS
                     LAIGIGLQNIPEGLAIALPLLREGFPHWKAFLVALATGLVEPIGGLIGIGLVQVARPL
                     LPYTLAFAAGAMLFVIAYEIIPESQNNHGNSKLATHTLLIGFVVMMFLDVVLS"
     gene            complement(2083..3075)
                     /locus_tag="VFC74_01190"
     CDS             complement(2083..3075)
                     /locus_tag="VFC74_01190"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004843514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HZK23982.1"
                     /translation="MTQTIKVGIVGYGNLGRGVEAAIAQNPDLELAAIFTRRSPQSLQ
                     INNQQAAVLHIDAAEQYQDKIDVMILCGGSATDLPAQSPIFAAHFNIVNSFDTHAKIP
                     EHFQAVDAVAKANGTTAAISIGWDPGLFSLNRLLAGAVLPEGRDYTFWGRGVSQGHSE
                     AIRRIEGVKYAVQYTIPIPEAMERVRKGENPELTAREKHLRACYVVAAAGADTAQITQ
                     IIKTIPNYFAEYDTEVNFISEEEFKQQHSKMPHGGFVIRSGRTGLQKENQQMLEFSLK
                     LESNPEFTSSVLVAFARAVYRLNREGQTGAKTIFDIPLGYLSHRPAADLRRELL"
     gene            complement(3149..4498)
                     /locus_tag="VFC74_01195"
     CDS             complement(3149..4498)
                     /locus_tag="VFC74_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK23983.1"
                     /translation="MKSYDLLSLAYRNLWRRKGRTILTVLGVIIGTTAIVVMLSLGIG
                     LVDSQKKQMEQWGSLNIIRVQQSYSFPGEPSEEQQRQLTDEAIEEIKEMEGVLAVSPA
                     YEVGGEAALGKKVGYFQLVGIEPAMMELLEFKVGHGRLLAADDKFNVVAGSWVINNFQ
                     DPKMAAGRWEEPPEADPLELLHQRISLTLLNSRQQKRIYNLNVVGILNDDSMENAYSV
                     FGPITEIKKMRDFVEQGSQDAPIYPPEKDVGVPLQPRTNRQAVVDQYSFALVRTTDVA
                     LTRNLSQELRDRGFNAYSMADSLEGIEDVARQMQAVLGGIGAITLLVAAIGITNTMVM
                     SIYERTREIAIMKVVGASFADVRTLFLTESSLIGIIGGTIGIGFSYFISYLLNTYIGA
                     AMGSGMGMENTPMQVSLIPPWLVLFAVAFSMGIGLISGLYPANRAIHLDPITAMRHG"
     gene            complement(4485..6140)
                     /locus_tag="VFC74_01200"
     CDS             complement(4485..6140)
                     /locus_tag="VFC74_01200"
                     /inference="COORDINATES: protein motif:HMM:NF019325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CARDB domain-containing protein"
                     /protein_id="HZK23984.1"
                     /translation="MPRRKPVLITLLLCLFCIAGIAQATETEATLPTEADATTISLLT
                     EGQQEVMAGEEFAVKAAITRHTAGEIKLVALSSANETLAQSELIAGLVPGASREVFLY
                     GLSEKPGLKDISQIQVVDSQDEVLETIYVDALLIQVQAKPAVENTLSLQLNTVAVSPY
                     KNNEFTLKFTLKNPGTAAAQNVSVQFNGEQAFVRGSSNVVQFSDIAAGASQAITVIMG
                     LSAANSAVYAIPVTINCVLPTGEKVSLSETITVTANDLGIKLTAPPAGTPRVFLQKYT
                     LSAHSILAGDTVKLTLYVQNSSSREVRNLKISLAAIPAEDSTSGTVFSPVNSSNSFFV
                     ERIAAKSTYTKSIEIYVDPNATAKTYLVPVEILYEDQQGTGYTVSEMVNIPVLQESRL
                     QVLSVEVPQLAPLGEPCPVTAEFVNVGKVALKNLMVTLEGDFPKENASFFLASFEIGQ
                     SEFYQAYITPQTEGPLEGKVIFTYNDNSNQEVMTEWPFSLEVQQMAMPGTGKPEEPLP
                     MDTATKGPSYVTILVTLLLLAVLVGVVLWRRRLKRGELFDEEL"
     gene            complement(6133..6831)
                     /locus_tag="VFC74_01205"
     CDS             complement(6133..6831)
                     /locus_tag="VFC74_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007717989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK23985.1"
                     /translation="MPLLELRSVVKKYRVGKEIITALDGVDLAVEAGEFVAILGTSGS
                     GKTTLLNVAAGLEKTTRGEVVFRGVVLNKLKERQKVAFRRKQMGFIFQSYNLIPALTA
                     QENVALPLIFDNVPPRERISRAVTVLKHMGLKDRRKHKPTEMSGGQQQRVAIARALVH
                     QPRIIFADELTGNLDSHTTHEIMCSLQATVKEQGQTLLLVTHDRDVARFADRVIFMVD
                     GKIVSEERGGADNA"
     gene            complement(6946..7845)
                     /locus_tag="VFC74_01210"
     CDS             complement(6946..7845)
                     /locus_tag="VFC74_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK23986.1"
                     /translation="MTAKVILNPYSNRWQAKKRWPEAEAALQAADVSFSLSVSQHQGQ
                     AVELAREAARAGYSPIIAAGGDGTIGEVVNGLAEAKGEEHLGTLGIMPLGTANDLVCN
                     LGLPLALGEAAKVIAAGQIQALDVCKANERYFVNNSAIGLEPYVTTIQQEIKVLKGIP
                     RYLVAALKGIFQHPTWQARVVWDDGEYTGPLSLISVGNAPRTGGLFYMAPHADPFDGK
                     LTAVIAYRKSALSTLLLLPKAMSPKGTFVESAGIREIHATQLRISLDRPSPAHCDGEL
                     FPQEVQELTYTILPGRLNVLSAK"
     gene            8101..8658
                     /locus_tag="VFC74_01215"
     CDS             8101..8658
                     /locus_tag="VFC74_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23987.1"
                     /translation="MKFTMKVFVALMLFALTGSSSLQAIAQQPASPAPAVGPTLIAAT
                     TGLTQEEYAEKAAQAHYITVQIFSEAFEAEFNNSPKLPWSEVRPQLLPYWSEELVDND
                     LKSFYNDHLWDWGYEMGFAFPLWQPELIEDVQITTNQQNVIIGQIKIPTNYDTVEYLP
                     ITLQNSNGNWVIASPFISNSTCELI"
     gene            complement(8869..9168)
                     /locus_tag="VFC74_01220"
     CDS             complement(8869..9168)
                     /locus_tag="VFC74_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23988.1"
                     /translation="MELTSDYRVNDVRKNSRVHNNNEVSTEKELNAAFLHNMAFTNSL
                     LMPAGYVISYDVRTDHRAHASNDTRTEKELNAAFMMNMSILNSLMMPAGFPISIF"
     gene            complement(9259..9429)
                     /locus_tag="VFC74_01225"
     CDS             complement(9259..9429)
                     /locus_tag="VFC74_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23989.1"
                     /translation="MEITSPYRVKDASIYEKAFAGRAVTTKEEKLAKEYLRNMARLNL
                     LAMPAGYAVSYF"
     gene            9758..11374
                     /locus_tag="VFC74_01230"
     CDS             9758..11374
                     /locus_tag="VFC74_01230"
                     /inference="COORDINATES: protein motif:HMM:NF037653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK23990.1"
                     /translation="MLTLQKLVNNLSKYEPKSRIIIDLQVRSFKLFEEGFTHYATDTI
                     YVGKVSDLPPTKIFTAQVNLLLLKDSELPFTFYENPLINYILIEDQHQIIKIINDLQD
                     FFNTREFIAKAIKKLLAIVMKKADLQEMLETAHQLLGNPLTLTDSTHHLVAHAGWGSE
                     LDEPEWQHYLEHGFMSNRFAIAVGNDLEFRRKANSDIIIPLVIQYEDILKHPQMVGRV
                     TAEGTNIAYLAMLEVNRPFTETDYELVPIVCDFISILMQQSQDKLNVATSETESMIIN
                     LLNGTYDPRTAHKKTHSHQLKLADELCIICISVDEVVDSTDKTVLVKSLMQNFFVGSI
                     TVIYNGSVVIINEYRHRDQLFFSKDKTSAFKQLLLAHNLRAGVSRPFYHLSDIKQHFQ
                     QAQNAINTASQLAPEECLYFYEEYFFHYLVTRLLENDSIASICHPKFLQLIKRDHEKA
                     SSYALSLYLYLQNNGDIKATAQAMHLHYNTMKYRLQRISDMMDVDLNNYDILLKCKLY
                     FLALENYHKIAFTDYFNQVRIPEETPYLEK"
     gene            complement(11444..11737)
                     /locus_tag="VFC74_01235"
     CDS             complement(11444..11737)
                     /locus_tag="VFC74_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23991.1"
                     /translation="MRRYFIYGCVVQLIIIIISAIAQDSEILVKGNYLLLVVMLLPVV
                     LAGRGALDPASHSVFVKLLTFLVPSFLLLGFYFAKFGLPQGVLTFWQTLRDAN"
     gene            11997..13361
                     /locus_tag="VFC74_01240"
     CDS             11997..13361
                     /locus_tag="VFC74_01240"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HZK23992.1"
                     /translation="MKKRKRLFIFIVSLVLTILVLPKFFSPTKQPLELLTFSPQGEKV
                     DVDTVITLELTAPPDIATLEQHFTINPAVPGQLTVDDSTILFTPEAPLQYSTHYAVTV
                     TEGVQTKQGSVLDEPLTLAFTTTDQPPLTIMAMGDIMLDQLTRQKLKEYDPTYPFLRV
                     KELLKQGDVIFGNLETPISARGTPLAGKKYTFSAAPFSVQCLEDAGINMVSLANNHIM
                     DYGEEALSDTLNILAEHNITSAGAGMNSTQAHSGTILEIQGYRVALLAYTDDFAVPAQ
                     HRALWQASEQKPGAAIVHDTNRIKADIQRLRTKADLVMVSFHWGYEYTTNVAAEQQKL
                     GHLAIDAGADLILGHHPHVPQGVEIYKGKPIVYSLSNFVFYPFANRPETQDTFILQAK
                     YENGRITALSLIPILGGNSQPYLPQGQELDALTSRLAQLLPRLGTSFEVTTDSIIQIK
                     LPVD"
     gene            complement(13464..14525)
                     /locus_tag="VFC74_01245"
     CDS             complement(13464..14525)
                     /locus_tag="VFC74_01245"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK23993.1"
                     /translation="MGRDSKFSKDELKVKELVPAMFGPPTEVFTTPITSKENALLLYQ
                     GKTPMWSPFSFGEHTMIMVDCDPENQARSNKESGGVDGYGVEWVFVEVVGGAIVKPGS
                     PKVKDINEWEKYVSIPDPDTWDWEGCYERNKEKLSADRMTYIAVPGCLFERLIAVMDF
                     DNAAVALIDDEQKEGVHRFFRAVTEVHKKFYSNVKKWFNADIVNFNDDWGSQRSEFFK
                     VETVREMLLPYVKEIIDHVHSLGMYMDLHCCGFVERFVPLFIEAGYSSWGGQPLNDKY
                     ALKQKYGDRFIFTDGPICPEDATEEEMDAAVADFMAKSGKDNRVLVDLMRAPKLRAKI
                     YEATRRNYDRLVAEGKAIL"
     gene            complement(14679..14878)
                     /locus_tag="VFC74_01250"
                     /pseudo
     CDS             complement(14679..14878)
                     /locus_tag="VFC74_01250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003457984.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2809 domain-containing protein"
     gene            complement(15126..16484)
                     /locus_tag="VFC74_01255"
     CDS             complement(15126..16484)
                     /locus_tag="VFC74_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK23994.1"
                     /translation="MSVHKPLTNKDINLFSIGLSPFMMLMGPVGAYFAYFLTDIVKMS
                     AAVVGGLLLLTRIADTVSIVLVGAVIEKTNLKWGKYCSWIYIGAPLTLIFISLLFTNP
                     SFMSETVKYVYYCVAYVLGSFALNFAFSGASGITPIMAIDPKSRVILSARRMQVSMIA
                     MLVYSSCHMPIILYFGRTNEGIGYLTYIIIAGFLQLFGYLLAARVAKPYDVMEEIPGT
                     TPSLGDMWRQVIGNPPLLVLMVYGMFTGTILSIAGGLNVYFFKYVAGNMLLISVFMTV
                     TSIVGIIATFVGQFVAQKVESKRTVMMIGSAISLVSLVAAYFCARDTTLYMICRWVGG
                     FAGLAGVVNFANYSDVADYAEWKTGKNAKGLIMSISALPMKIAAAVAGAITGFGLAAI
                     GYRAEAAATPQLIQGIIVLSTLLPAVCAFISLITLTFYKLDNQTMAKIHQDLAARNTA
                     AH"
     gene            complement(17027..17437)
                     /locus_tag="VFC74_01260"
     CDS             complement(17027..17437)
                     /locus_tag="VFC74_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010246465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23995.1"
                     /translation="MNRKLMTKIKEISDTHKVIMRGYVQLSGGVHAILFGHYCINTLF
                     YKDLYRVTRDVLRVNQMANRNLRQVKKLLKKQGYRKIWTKGIFSVYGDLRPLAVAAGL
                     GRWQNGIISNDKYGTDFLISGIFFKGKPKNTRLL"
     gene            complement(17778..18206)
                     /locus_tag="VFC74_01265"
     CDS             complement(17778..18206)
                     /locus_tag="VFC74_01265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK23996.1"
                     /translation="MEGLGLEPYAAIISWSLQQEQPLEVWQTLIAEYLEQLGQVCAAD
                     DKRILGHIKCLVRFPAGGHLRGSKVSVAYPADVEFKEKETAAYANIKLTLNILVYGLP
                     NQEAQKVVRNVGSVLAANWQAKMEILSISHHDDHQHDYHQ"
     gene            complement(18194..18793)
                     /locus_tag="VFC74_01270"
     CDS             complement(18194..18793)
                     /locus_tag="VFC74_01270"
                     /inference="COORDINATES: protein motif:HMM:NF014542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK23997.1"
                     /translation="MHLLMITGFLGSGKTTVAIQVASECIQQGRKMAILVNEIGEVGI
                     DGELMRELELNVYELANGCICCTLASDLVPTLEKLNQEYAVDLVLIEPSGTAEPSSML
                     NVLQYYRGRPLESVFITAVLDSLRLPELYEIVTPLITRQIKNANILLVNKVDLATAEQ
                     LQDVHRIVEEINPEVKRYLVSARQGLAKELLKEILPWKD"
     gene            complement(19063..20172)
                     /locus_tag="VFC74_01275"
     CDS             complement(19063..20172)
                     /locus_tag="VFC74_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK23998.1"
                     /translation="MTVEELYNQRLQRIKTAMDMGTPDRIPISLGGPSFIYYSEPEAT
                     LSDYILRPKWADDAVIRSYKMLPEIDTGPMLGGSREGMGAMWYSKTKLPGKELPPNAL
                     WQIVEVGPMTEDDYDTIINKGWAALGKELLDRLGYTEEQLAPDFEYMDKMNKVVREEL
                     GLVNMSGGAYVMPVEILSAARSLKKLLRDLYRMPDKVLEAMEIIADEYIESATKLIRE
                     RQPLSVFSGHGRFGDDLFNVKNFEKFTWRFTKKTADAFIAAGTKVLFHMDGKWDNYLS
                     YWRDFPKGSCIFDPDSLTDIVKIKEVLGDYMCVTGDVPPAVLEMGTPDDNYNYARKLV
                     ELFGKSGFIMSSGCSVPPYAPLDNIKAVIAATLEG"
     gene            complement(20394..21008)
                     /locus_tag="VFC74_01280"
     CDS             complement(20394..21008)
                     /locus_tag="VFC74_01280"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK23999.1"
                     /translation="MEELIALMADLEEDQLLQRIKERLDAGEDAKALFDACQQGMAVV
                     GERFESGEYFLSDLIMSGEVFSAVSEVITPYFAEGAGSEPKGKVVIGTIIGDIHDIGK
                     DIVIVLLQSAGYEVIDLGVDVPVERFVEAVKETGAPVVGISGLLTVAFPPMRECADAL
                     KAAGLDVKVMIGGAPVTADICQFVGADAFGTASDAVNFCKEWIA"
BASE COUNT         6326 a         5271 c         4193 g         6055 t
ORIGIN      
        1 tatataaaaa ttcgctatat ttacagcaaa aacctgctta acaacagcaa ttaaaaaacg
       61 ggcgaactca gttcgccctt acggaaagca gcaacggcag accgttgtct gcattttatt
      121 aatcacagtt atagacgcaa ggtattattg tttaagtaat gatttcaatt gcgcaaaggg
      181 gagttgtccc ggcgctgaag cggcttgtcc cgaagcaaaa gtgattgccg aaccaaaggt
      241 agacccagcg aggcgactga tcgtgcctag cgcggacatc gaaatagcta caacgggaac
      301 ttgcgcagca tcagtggcaa aggtcagagt ggcagttaac aagtttaaga catcttgggg
      361 ttcttgcggc atcacagcaa ttttaatgat atcagcacct gttttttgta attgcagcag
      421 tttagcaaat agggcgcttg gtgctggagt ttgcgcaaaa ttgtggtagg acactattac
      481 atgggctccc tgctgctgtg cagctgtaag caatggtaca agcttatcct gacccaaact
      541 atattccaca tcaataaaat cagtttgccc cgtttgcagt aatgtctgta gaagggccat
      601 acggaaaggc tgcgatagtt cttggcagcc gccttctacg tggtcccggc aggtcacgat
      661 taagggacaa gcagggagaa ctgtacgcag tttaaaaagg gcagcagtaa ctgcagctag
      721 atttttagct gcccgaaaat aatcaactcg ccactcgact aaatctggct gtaatcgtgc
      781 cattgcttca gcctctgcca gcagttcagg taaagttggg ctgagcagcg gcacacaaat
      841 attagtgtta ctaccaccaa taatccgtcc tttaacgtga agctcttttt tgctctgcat
      901 ttgttcacca actcagacta tattacaggt aggggcgaac tgtgttcgcc cgcttttctg
      961 tcaaccatct aagatgtacg ggcgcacata gtgacgcccc tacatgttta ttgttaaaat
     1021 catatctaga attatgtcag ctgtcaaatt aattttggtg tttgctgact tttacagtgt
     1081 caacttaaga ccacatctaa aaacatcatc actacaaaac ctattaataa agtatgggtt
     1141 gctaatttgg agttgccgtg attgttctga ctttcaggaa taatttcata ggcaataaca
     1201 aaaagcatgg ctccagcggc aaaagccaag gtgtaaggga gtaaggggcg tgctacttgc
     1261 actaaaccaa tgcctatcag acccccaatg ggttctacca gacctgtagc caaggcaact
     1321 agaaaggcct tccagtgtgg gaacccctcc cgtaataaag gtaaggcaat agctaaacct
     1381 tcagggatat tttgcaggcc aatgccgatg gccagagata atccattagc tatttgacca
     1441 tcgccaaagc cgacccccac ggccatgcct tccgggaaat tatgaatagt aatagctaag
     1501 acaaaaatcc agacccttct taaactaggg ctcatcgtta gcagcttact gctatgcttt
     1561 tttgtttcag gcactgggcc gacctggagg ttagtattgg ggaaaaaggt gtccacaaca
     1621 tctaaaaata tgcctccggc caaaatacca acaatggcaa tgctagctcc cttaatacct
     1681 tggccgcccg cttcaatcgc cgggataatc aggctaaagg aggtagcggc cagcatcact
     1741 cctgccgccg cacctaacag agcatcaagg accttttttg gaacttcttt gacgaagaaa
     1801 attggcagac aacctacacc tgtggctagc cctgctgcca agctagccaa gactcctacc
     1861 caaatgatat taaactgtga taagtacgct accatataat gtagtcacct ctcgcattat
     1921 actatctaac ctttatcatc atatggcaga gcaattttga tggtgagagg tgagtgataa
     1981 aaaagcttgt gcttaattct acctaataat aaagacgctt cgcaaaaaag cgaagcgtcc
     2041 ccatctaaac tgcatagttg ttcatcaaaa ttgagtgctg ttttacaata attcccgccg
     2101 caggtctgcc gcaggccggt gggataagta gcccagcggt atatcgaaaa ttgtcttagc
     2161 ccctgtctga ccttcccggt ttaaacgata gactgctctg gcaaaagcaa ctagcacact
     2221 tgaggtgaac tccgggttgc tttcaagctt taaagaaaac tcaagcattt gctggttttc
     2281 tttttgcagc cccgttcgtc cactgcgaat tacaaaaccg ccgtgtggca tcttgctgtg
     2341 ctgctgcttg aattcttcct cactgataaa attcacttca gtatcgtatt ccgcaaagta
     2401 gttgggtatg gtttttatga tctgcgtaat ctgtgctgta tcggccccag cagctgccac
     2461 gacataacat gcacgtagat gtttttctct tgctgttaat tctgggtttt caccctttct
     2521 aacgcgctcc attgcctctg gaataggaat cgtatactgc acagcatact tgacaccttc
     2581 aattcttctg atggcctcag agtgaccttg gctaacacca cggccccaga aagtataatc
     2641 acggccttca ggcaacacgg ctccagccag caggcggtta agtgaaaaca agcctggatc
     2701 ccagccaatg gagatcgccg ctgtggtgcc atttgcttta gccacggcat cgacagcttg
     2761 aaagtgttcg ggaatcttgg catgcgtatc aaaactatta acaatattaa aatgagctgc
     2821 aaagatagga ctctgcgctg gcaaatctgt tgccgaaccg ccacacaaaa tcattacatc
     2881 tattttatcc tgatattgtt ctgcggcgtc aatatgtaat accgctgcct gctggttgtt
     2941 gatttgcagg ctttgcggag agcgacgggt aaaaatggct gccaactcta gatcggggtt
     3001 ttgggcaatg gcagcctcca cccctcgtcc taaattacca taaccgacaa tacctacttt
     3061 aattgtttgc gtcatcttct tccccctgca gattattagt gaataatttt agtttatcat
     3121 acatctttcc cgctgccgtc acaagtcatt aaccatgccg cattgcagtt atgggatcta
     3181 gatgaatcgc cctgttggcc gggtaaagcc ctgaaatcag accaataccc atactaaaag
     3241 cgacggcaaa caaaaccagc cagggcggaa tcagcgaaac ttgcattggt gtattttcca
     3301 tgcccatacc actgcccatc gctgcaccaa tataagtatt caaaagatag gagataaagt
     3361 aactaaaacc tatgccaatc gtacccccga tgataccaat taatgatgat tcagtaagaa
     3421 acagggtgcg aacatcggca aaagaagccc ctaccacctt cataatggca atttcccgtg
     3481 tgcgctcata aatagacatc accatggtat tagtaatacc gatggcggct accagcaaag
     3541 tgatggcacc aataccacct aagactgcct gcatttgtcg agccacatct tcaataccct
     3601 ctaaactatc agccatggaa taagcattaa agcctcggtc acgtaactct tgactaaggt
     3661 tacgagtaag ggctacatct gtcgtacgta ctaaagcaaa ggaatattga tccacaactg
     3721 cttgccggtt agtccgcggc tgcagaggca cacctacatc tttttccggt ggatagatag
     3781 gtgcatcttg actaccctgt tccacaaaat cacgcatttt tttaatttcg gtgatgggac
     3841 caaagacaga gtaggcattt tccatagaat catcattcag tattcccaca acattaaggt
     3901 tataaatacg cttctgctgt cgtgaattca gcagcgtgag agagatgcgt tgatgcagca
     3961 gctcaagggg atcagcctca ggcggttctt cccagcggcc agcagccatt ttagggtcct
     4021 gaaaattatt aatcacccag gaacctgcca ccacattaaa cttatcgtca gctgctagca
     4081 aacgaccatg gccaacttta aattcaagta gttccatcat cgccggctcg atgcccacta
     4141 actggaagta accaaccttt ttacccagcg ctgcttcgcc tcctacctca taggcaggcg
     4201 aaaccgccag cacgccctcc atctccttaa tttcttcaat ggcctcgtct gttagttggc
     4261 gttgctgctc ttcactaggt tcaccaggga atgaataaga ctgctgtacc cgaattatat
     4321 tcaggcttcc ccattgctcc atttgctttt tttgtgaatc caccaggcca atacctaagg
     4381 ataacatcac cacaatagct gtggtgccaa taatcacacc taaaactgtg aggatcgtcc
     4441 gtcctttgcg gcgccacaaa ttacgataag ctaaagacag taagtcataa ctcttcatca
     4501 aaaagctctc cacgcttcag ccgacgacgc cataacacca ccccaactag tacggcaagc
     4561 agtaacaaag taacaaggat tgtgacatag ctagggcctt tagtcgccgt atccatgggc
     4621 agcggctctt ccggctttcc cgttcctggc atcgccatct gctgcacctc caggctaaaa
     4681 ggccattctg tcataacttc ctggttggag ttatcgttat aggtaaagat aacctttcct
     4741 tccagtggac cttctgtctg gggtgtaata taagcctggt aaaactcgct ctgaccgatc
     4801 tcaaaacttg ccaggaaaaa tgaagcattt tctttgggga aatccccctc taaagtgacc
     4861 attaagttct ttaaagccac cttacccaca ttaacaaact ctgccgttac aggacaaggt
     4921 tcgcccaacg gggcaagctg cggcacctct acgcttaata cctgcaagcg gctttcctgc
     4981 agcacgggga tatttaccat ttccgatact gtatagcctg tgccttgttg gtcttcataa
     5041 agaatttcca caggcaccag ataggtttta gctgttgcat tgggatccac atatatttca
     5101 attgacttag tataggtact tttagccgca atgcgttcca caaaaaaact attactgctg
     5161 ttgacaggtg aaaagactgt gccgctggta ctatcttcgg caggaattgc ggctaaagat
     5221 attttcagat tgcgcacctc acggctgctg ctgttttgca catataaagt cagttttaca
     5281 gtatctcccg ctaagatact atgtgctgat aatgtgtatt tttggaggaa gacgcgcggt
     5341 gtccccgcgg ggggtgccgt tagcttgatg cctaaatcgt tagctgttac cgtgattgtt
     5401 tcagacaagc tgactttttc gccagtgggc agtacacagt taatagttac agggatcgca
     5461 tagacggcac tattagcagc agacaatccc atgattacag taatcgcctg actagcgccg
     5521 gcggcgatat ctgaaaactg cacaacatta ctactgccgc gcacaaaggc ttgctcacca
     5581 ttaaattgca cagatacgtt ttgcgcagct gccgtacccg gattcttcaa ggtaaacttt
     5641 aaagtaaact cattgttctt atatggagaa accgcaacgg tatttagctg taaactgagg
     5701 gtgttctcta cagctggctt ggcctgcact tgaataagga gagcatcaac ataaatagtt
     5761 tccagtactt catcctgact atcaacaact tggatttggg aaatatcctt aagccctggt
     5821 ttttcactga gcccatataa aaacacctct cgagaggcac ccggtaccaa tccagcgatt
     5881 agttcactct gagccagtgt ctcattagcc gaggaaagag cgactaattt gatttcgccg
     5941 gcagtatgac gtgtaatagc tgccttgact gcgaattcct cccctgccat cacttcttgt
     6001 tgcccttccg tcaaaagaga aattgttgta gcatcagctt cagttggcag ggttgcttct
     6061 gtttcagtag cttgggcaat cccagctata cagaataaac ataacaacaa tgtaattaaa
     6121 acaggctttc ttctaggcat tgtcagcccc tccccgttct tcagatacga tcttaccatc
     6181 taccataaaa ataaccctgt cggcgaaacg tgccacatca cgatcatggg ttaccagcaa
     6241 gagcgtctgc ccttgctctt taactgtggc ttgcagactg cacattattt catgggtggt
     6301 gtgggaatca agattccccg ttagctcgtc ggcaaatatt atgcgcggtt gatgcaccag
     6361 ggctctggca atagctaccc gctgctgttg accaccactc atttctgtcg gtttatgctt
     6421 acgacgatcc tttaaaccca tatgtttgag gacagtcact gcccgactga tgcgttcgcg
     6481 tggcggcaca ttatcaaaaa tgaggggtaa agccacattc tcttgtgctg taagagctgg
     6541 aattaaatta taagattgaa agataaagcc catttgtttg cggcgaaaag ctactttttg
     6601 tctttctttg agcttattta aaacgacacc acgaaatacg acctcaccac gggttgtttt
     6661 ttccagccca gctgctacat taagcagtgt cgtcttgcca ctgcccgagg tgccgagaat
     6721 ggcgacaaac tctccggcct ctacagctaa atctacccca tctaaagctg taatgatctc
     6781 tttgcccaca cgatattttt tcacgacgga ccgtaattcc aagagcggca accaattcac
     6841 ctcacagaaa ggtatttatg atctctatag attgattatt ttgcaaaacg ttccaatatt
     6901 cctgcaatta tggccaggct tgatcaattt tacacaatag gtaatttact tagcgctcaa
     6961 tacgtttaac ctgcctggca aaatggtata ggtaagctcc tgcacttcct gtggaaataa
     7021 ctccccatcg cagtgtgcag gggaaggccg gtcgagacta atacgaagct gagttgcatg
     7081 aatttcacga atacctgcag actccacaaa tgtgcccttg ggagacattg cttttggtaa
     7141 aagaagtaga gtgcttaaag ctgattttct ataagctatc acagccgtta gtttaccatc
     7201 aaagggatcg gcatgcggcg ccatataaaa taagccgccg gtacgaggtg cattgcccac
     7261 agatattaaa gaaagcggtc ccgtatactc cccatcgtcc cacaccaccc gcgcctgcca
     7321 agttgggtgc tgaaaaatac cttttaaggc ggctactaaa tagcggggga taccctttaa
     7381 caccttaatt tcttgttgaa ttgtcgtcac atatggctct aaaccaatag ccgaattatt
     7441 aacaaaataa cgctcgtttg ctttacacac atctaacgcc tgtatttggc cagcagcaat
     7501 taccttagcc gcttccccta atgcaagggg cagccctaga ttacaaacta aatcattggc
     7561 agtaccaagt ggcataatcc ccaaggtacc caaatgttct tcgcccttgg cttctgccag
     7621 accattaact acttcgccaa ttgtaccgtc gcccccagca gcaataatgg gggagtaacc
     7681 ggctctcgcc gcctctcttg ccaattctac ggcctgtccc tgatgttggg atacagacaa
     7741 cgagaaacta acatctgccg cttgcagtgc tgcttctgcc tccggccagc gttttttagc
     7801 ctgccagcga tttgaatagg gatttaagat aactttagct gtcatattta tttccttttc
     7861 ttagctcgtt ttagcttttt atagttattt tactttaaaa tactacttct atattccata
     7921 gcaagtcaaa tattcctgct ctggtcaaaa taggctgcct tattattgtt tacctgctga
     7981 ttattaagta accttaaaag agtatttaga agatgtaatc gattattcat actcagcaaa
     8041 cgtcttttac ttataatgaa agcagcaatc aaaataaaaa taatatcgga ggggtacata
     8101 atgaaattta caatgaaggt ttttgtggcg ttgatgttat ttgccttaac aggcagcagc
     8161 agtttgcagg ctattgccca acaacctgcc tctccagcac ctgctgtagg gccaacgctg
     8221 atagcagcga ccaccggctt aacgcaggaa gagtatgctg aaaaagctgc tcaagcgcat
     8281 tacataaccg tgcaaatatt tagtgaagca tttgaagctg aatttaataa ttccccgaaa
     8341 ttaccctggt cagaagttcg tccccaacta ttgccatact ggtcagaaga actagtagac
     8401 aatgatctaa aatcatttta taatgatcat ttatgggatt ggggctatga aatgggcttt
     8461 gccttcccct tgtggcagcc agaattaatt gaagatgtac aaatcaccac taatcagcaa
     8521 aatgtaatca ttggccagat taaaattccc actaactatg atactgttga atatcttccc
     8581 attacactac aaaatagcaa tggcaactgg gtcattgcct cccctttcat aagtaactct
     8641 acctgtgagc tcatctagca tcctcgtaga taatgatata actcttgttt agtaaaacaa
     8701 ccacactcac gtgtggttgt tttactactg tactgccctg cagctgtgga catggaaaag
     8761 agtgccgctg gcggcactct tttcgcatag ggtgttgctg caaattgtgg tggggttaat
     8821 tagtgggatt tcgtgatgtg atttatagta aagctggtgt tatttttttt agaagatcga
     8881 aatcggaaaa cctgctggca tcattaggga gttaagtata gacatgttca tcataaaagc
     8941 tgcgtttaat tccttttcag ttcttgtatc attactggcg tgtgccctat ggtctgttct
     9001 aacatcgtag gaaataacat agcctgctgg catcaatagg gagtttgtga aagccatgtt
     9061 atgtagaaaa gctgcgttca gttccttttc agtgcttacc tcattattgt tgtgtaccct
     9121 actgtttttt ctaacatcat tgaccctgta atcagaagtg agttccatta aaatagcctc
     9181 cttaaaattt tgttttttta tctcagatta aactattgag tgttccagct agttattttt
     9241 gtggttgttt tgtgttttct agaagtagga aactgcatag cctgctggca tagctagcaa
     9301 gtttaaacgt gccatattcc tcaaatattc cttagctaac ttttcttctt tagttgtcac
     9361 agcacggccc gcaaatgctt tttcgtaaat gctggcgtcc ttgacccgat aaggcgaagt
     9421 gatttccatt tgtgtagcct ccttactttt aggttcatct tctgacttta gtttacaaga
     9481 ttattagcag caaaactacg ttgtatatac agaactttac atgtaaacat tgctaaatct
     9541 accaatgctg gttttatatt ttattaccac tttcatcctt taattttatt attttataca
     9601 tgcttgctag gatatcatta gattattatc ctatctatta ccatttaact tattacttgg
     9661 tggcaagatc agctattcca tgttttacat tagttattgt tcacaagtgc gataattatt
     9721 gatataatta ggacagacat ctaaaggagg taagctgatg ttgacattac agaaactagt
     9781 aaataattta agtaaatacg aacccaaaag cagaattatc attgatctgc aagtacgttc
     9841 ttttaagctc tttgaagaag gttttactca ttatgcgacc gacaccatct atgtagggaa
     9901 ggtctccgac ttaccaccaa ctaaaatctt caccgcacaa gttaatttgc tcctcttgaa
     9961 agatagcgaa ttgccgttta ctttttacga aaatcccctg atcaattaca ttttaattga
    10021 ggaccagcat cagataatca agattattaa tgacttgcag gattttttta acacacggga
    10081 gttcatcgct aaagcaataa aaaaactttt agcgatcgtc atgaaaaaag ctgatctaca
    10141 agagatgctg gaaacagcgc accagctttt aggcaaccca cttaccctaa ccgactccac
    10201 ccatcacctt gttgctcacg ctggctgggg cagtgaacta gatgaaccag aatggcagca
    10261 ctatttagaa catggcttta tgtctaatcg ttttgctatc gccgttggta atgatttaga
    10321 atttagacgt aaagccaaca gcgatattat catcccactg gtcatacagt acgaagacat
    10381 tttaaaacat cctcaaatgg ttggtcgtgt tactgctgaa ggaactaaca tcgcctatct
    10441 cgctatgtta gaagtcaatc gccccttcac agaaaccgac tatgaactag tgcccattgt
    10501 ttgtgatttc atctctatcc tcatgcaaca gtcccaggac aagctgaatg tggccacttc
    10561 agaaactgaa tccatgatca tcaacttgct aaatggcact tatgatccaa ggactgcgca
    10621 taaaaaaaca cacagtcatc agctaaaact tgccgatgaa ctatgtataa tttgtattag
    10681 tgtcgatgaa gttgtggatt caaccgataa aacagtttta gtaaaaagct tgatgcagaa
    10741 cttttttgtc ggcagtatca ctgtgattta caacggcagt gtggtcatca ttaacgaata
    10801 tagacataga gaccagctct tttttagtaa ggacaaaaca agcgctttta agcagctgtt
    10861 attggcccat aatctccgtg caggcgttag cagaccattt taccatttgt cagatattaa
    10921 acaacatttc caacaagcgc aaaatgccat caacactgcc tcacagttag cacctgaaga
    10981 atgcctatat ttctatgaag aatacttctt tcactatctt gttacacgtc ttcttgaaaa
    11041 cgatagcatc gctagcatct gtcaccctaa atttttgcag ctaatcaaac gcgaccacga
    11101 gaaagcaagc agctatgcgc ttagtctcta tctatatctg caaaacaatg gcgatatcaa
    11161 ggctacggca caagcaatgc acctacacta taatactatg aagtatcgcc tacaaaggat
    11221 ttctgacatg atggacgttg acttaaataa ttatgacatc ctcctaaaat gcaaactcta
    11281 ctttctggct ttagaaaact atcataaaat tgcttttacg gattacttca accaagttcg
    11341 catcccagaa gaaacacctt atctcgaaaa atagaatcta cgggtcggcg taccttgaac
    11401 accttgcaaa attaaaggag catccctttt aacaaccaag ttgttaattt gcatcgcgca
    11461 gtgtctgcca aaaagtaagc acaccttggg gcaaaccgaa cttagcaaaa taaaaaccga
    11521 gcagcaaaaa acttggcacc aaaaaagtga gaagttttac gaaaacacta tggctcgcgg
    11581 gatctaaagc acccctgcca gcgagcacta ctggcagcaa catgactact aaaagcagat
    11641 aattaccttt tactaatatt tcactatctt gcgctattgc actaatgatg ataataatca
    11701 actgcacaac gcagccatag ataaagtatc gtctcattga tgacacctcc acatgggttt
    11761 ataaagccag ttgatatact atattcaaca accttctcta tttcctcctt atatttggca
    11821 attactttac ctttcgcctt cgacaggcct gacggccctg cttgtcacca taataaacac
    11881 accagcacca aatgcacaag cctcacaaaa gcccgcctaa tcaagggttt aataaatctg
    11941 gctttaccta acgacaacgc ctcataaagc gacagcaaaa tcaaggaggg agatagttga
    12001 agaaacggaa aagattattt atttttatcg tatctctagt gctcaccatt ttagtgctgc
    12061 ctaaattttt ttctcccacc aagcagcccc tagagttatt aactttttct ccgcaaggag
    12121 aaaaagtgga tgttgacacc gtcattaccc ttgagctaac agctccacca gatatagcaa
    12181 ccctcgagca acattttact atcaacccgg cagttcccgg acagctgaca gtggatgact
    12241 ccacaattct gtttacacca gaggccccac tgcaatatag tacccattat gctgtaacag
    12301 tgactgaagg tgttcaaaca aaacagggaa gtgtcttgga tgagcccttg acactggcat
    12361 ttaccacaac agaccaaccg ccactgacca ttatggcgat gggcgatatc atgctggatc
    12421 aactaaccag gcaaaagttg aaagaatatg acccgaccta ccccttccta agggtaaaag
    12481 aactgcttaa gcaaggcgat gtgatttttg gcaacttgga gacgcctatt tccgcaaggg
    12541 gtaccccctt agcggggaaa aaatacactt tcagtgctgc ccctttctcc gtccaatgcc
    12601 tagaagatgc agggatcaat atggtttccc tggccaacaa ccatatcatg gattatggag
    12661 aagaagcctt atccgatacc ctaaacattt tagctgaaca taatattacc tctgcggggg
    12721 cgggtatgaa cagcactcaa gctcatagcg gtacaattct ggaaatccag gggtatcggg
    12781 tggcgctgtt agcctatact gatgattttg ctgtgcctgc ccagcaccgt gctctttggc
    12841 aggcatcaga acaaaaacca ggcgcggcta tagttcatga tacgaacaga attaaagcag
    12901 atatccagcg gctgcgtaca aaagcagatc ttgtgatggt ctctttccat tggggctatg
    12961 aatacaccac taatgtcgcg gctgaacaac agaaacttgg gcacttggca atagacgctg
    13021 gtgccgatct gatcctaggt catcaccctc atgtgcccca aggagtagaa atatacaaag
    13081 gcaaaccaat cgtctacagt ctgagcaatt ttgtttttta tccctttgct aatcgtcccg
    13141 aaacccagga tactttcatt ctacaggcga aatatgaaaa tggacgaatc acagctctct
    13201 cccttattcc aattctcggt ggcaatagtc agccgtattt accccaggga caagagctag
    13261 atgctttaac gtcacgacta gcgcaacttt tgcctcggct cgggacttct tttgaagtaa
    13321 ccactgactc cattattcaa ataaaattac cagtagatta ggataacaaa gtgatcaaaa
    13381 gtgaactgaa ccactcgccc tgctcagaca gaaaaaaacg acgcaccact taataggtgc
    13441 gtcgttgctg tttaactgta tgcttacaaa attgctttac cttccgctac tagccggtca
    13501 taatttcttc tggtagcctc atatatttta gcccgtaact ttggtgccct catcagatcc
    13561 accaatactc tgttgtcctt tcctgacttc gccatgaaat ctgccaccgc ggcatccatt
    13621 tcttcttcag tagcatcttc cggacaaatc ggaccgtcag taaaaatgaa gcggtcacca
    13681 tatttctgct tcagagcata tttatcattg aggggctgac caccccagga actataacca
    13741 gcttcaataa acagcggcac aaatctttcc acaaacccgc agcagtgtaa atccatatac
    13801 attcctagag aatggacatg atcgataatt tccttaacgt atggcaatag catttctctt
    13861 actgtttcaa ctttaaagaa ttcggaacgc tgcgaacccc aatcgtcgtt aaagttcaca
    13921 atgtcggcat taaaccattt tttaacgtta ctatagaatt ttttatgtac ttcagttact
    13981 gctctaaaga agcggtgtac accttccttt tgctcgtcgt caatcagcgc cactgccgca
    14041 ttatcaaaat ccatcacagc aatcagtctt tcaaacaaac accctggcac agcaatatat
    14101 gtcattctat cagcagatag tttttctttg ttgcgctcat agcagccttc ccaatcccaa
    14161 gtatcgggat ccggtatgct aacatatttt tcccactcat taatatcttt cactttggga
    14221 ctacccggct taacgattgc accaccaaca acctcaacaa agacccattc gacaccgtaa
    14281 ccgtccacac cgccgctttc tttattgctg cgcgcctgat tctccggatc acaatcgacc
    14341 ataatcatcg tgtgctcacc aaaagaaaag ggtgaccaca taggtgtttt cccctgatac
    14401 agaagcaaag cattctcttt cgaagtaatt ggtgtcgtaa acacctctgt cggcggacca
    14461 aacatcgccg gcaccaactc ttttactttt agttcatctt tagaaaactt actgtctctc
    14521 cccatcgtca atacctccat agttaaactt ttcactaaca accatacttt tttaaatagt
    14581 tgctcttaag tgaattatat gactacttct cctgactgtc aataactgct gcgtgtcagg
    14641 ggacggatcc ttgtcacact gctattgcca tcattagctc actcagacgc cgtctgagtc
    14701 cgtttaaaga ttatatctac aattatacct atgatgattc ccacgaaata gcaaagcaaa
    14761 tcactccata aaaaacctag ccaagaacca aaccgccgag acgagtgctg cgtgaagcat
    14821 caatccacat cgcatgatag agttgactaa gctcaatcag ataagcaata ataaacgcag
    14881 cccaggctaa cctacttatc ccaacaccca ctgctttagc tagtccttac aaacctgact
    14941 tcaatttatc ttggggtcag aagctaaata gcagtacaac cccagcaatt tcctgccggg
    15001 gctgtactac ttaacttaaa cctcgcccct ctgtttaacc agtatacaga taagtctacg
    15061 tgattatctg tacgatgtgc tgaccagtaa ggttcatgta ttttccacct gcttcagcag
    15121 aacaattagt gtgcagcagt gtttctggct gctaaatcct gatgaatttt agccatagtt
    15181 tgattatcaa gcttgtaaaa agtcagcgta atcaggctaa tgaaagcgca gactgccgga
    15241 agcaatgtgg aaagcacaat aatcccttgg ataagttgcg gtgtcgctgc agcttctgca
    15301 cgatagccaa tagctgccaa gccaaaaccc gtaattgctc ccgctacagc tgctgcaatt
    15361 ttcatcggga gtgcactgat tgacataatt aaacccttag catttttacc agtcttccat
    15421 tccgcataat cagccacatc agaatagttt gcaaaattta ccacaccggc taacccggca
    15481 aaccccccaa cccaacgaca gatcatgtat aacgttgtgt cgcgggcaca aaaataggca
    15541 gcaactaaac taaccagact aatagctgaa cctatcatca taaccgtacg tttgctctct
    15601 accttttgtg ccacaaactg accgacaaaa gttgctatta tccctacaat ggaggtaaca
    15661 gtcataaaga ctgagatcaa cagcatattg cctgctacat acttaaaaaa gtaaacgttt
    15721 aatcctccgg caattgacag gattgtcccg gtaaacatgc cgtagaccat taatacaagc
    15781 aacggtggat taccaatcac ctgacgccac atgtccccaa gagaaggtgt agtaccaggg
    15841 atttcttcca tcacatcata gggctttgcc acacgagcag ccaggagata accgaacaac
    15901 tgcagaaatc ctgcaataat aatataggtg agataaccga taccctcatt tgtacgacca
    15961 aaataaagaa taatcggcat atgacaagag ctatatacta gcatggcaat catggaaact
    16021 tgcattcgcc ttgccgatag aattactctg gatttgggat cgatggccat aatcggcgta
    16081 atacctgaag caccactaaa ggcaaagttc aaggcaaagg aacctaaaac atatgccaca
    16141 caataataaa catattttac tgtttcactc atgaatgaag gattggtaaa caatagagag
    16201 ataaaaatta gcgttagcgg agcacctata tagatccagg aacaatattt gccccatttt
    16261 aggttagttt tctcaataac agcacccact aaaacaatag agactgtatc tgctatacga
    16321 gttagcaaaa gcagcccacc tactacagct gcactcattt tcacaatatc tgtgagaaaa
    16381 taagcaaaat aggccccaac cggccccata agcatcataa atggtgagag gccaatacta
    16441 aaaagattga tgtccttgtt cgttaatggt ttatgtactg acatttacaa ctacctcctt
    16501 gactttaaca tttcttctgt aatcaacatg ttgtttttaa cagaataggt gtctggtcgt
    16561 agttgaaaag ctgccgagtc aaaaacccgg ggaaaacatg attagtgcat cgaaccacct
    16621 ctttatactt tacctgccgc cgccggtaat agcaggcatc aaatgggaag tcacccgcca
    16681 ctagttgtcc tactattcta aaccttatgt atattgatgt taaataatta ctactttgct
    16741 catatttact gatcaagata ttaatctcca tttacattac cgatttatct gaatgaaata
    16801 actgttgcat ttagccgcga gcgcccttac agataatgtt aaagcataag acaagcaatg
    16861 ccaaatgggt gagtgtgcac gctaatccag tcccagtcta acgcttaaaa ccacttcagg
    16921 acgatgctca cgcctcgagc tggcagcagt atgcaaaaaa gaaacgacac accaaatagc
    16981 agtgcgtcgt taaaaatttt actggtaaag acctccttaa ccgggcttaa agaagtcgtg
    17041 tgtttttagg ctttccttta aaaaaaatcc cggagattag aaaatctgtc ccatatttgt
    17101 cattagaaat gataccattt tgccaccggc ctaaccctgc ggctaccgct aaagggcgta
    17161 ggtcgccata gacagagaaa attccctttg tccatatttt gcggtaccct tgctttttaa
    17221 gcagtttttt aacttgtcgt aaatttctat tagccatctg attaaccctt aaaacgtctc
    17281 tggttactct gtaaaggtct ttataaaata acgtatttat acaatagtga ccaaacagaa
    17341 tagcgtgtac tcctccgctc aactgaacat aaccacgcat aattacctta tgagtatcac
    17401 tgatttcctt gattttagtc atcagcttac gattcaatat caaaccaact ccttcattct
    17461 tttcttaaaa ggaaatcttc actgctctaa agttccacaa actaatcatt agtatgcttc
    17521 ccaggcttgc tctacttagc aagctgatat tatcttacca agtaaagtat atctttattt
    17581 ataggttgcg cacttatgct gctaaccaat attctaccgt catgtttcac gtcagcgaac
    17641 gcaaattact gatactcaag ctggttttat cattatcaac cagcacaatc tgcccaattc
    17701 tataaaagat aaaaataaac gacgccccaa cctaagttag tgcgtcgttt agaaacctac
    17761 ctttttacct taacgattta ttggtgataa tcatgctggt ggtcatcgtg atgtgaaatt
    17821 gacaaaatct ccatttttgc ctgccagttt gcggcaagaa cagaacccac gttccgaaca
    17881 accttctgtg cttcttgatt tggaagacca tagaccagta tatttaacgt caactttatg
    17941 ttagcatacg ccgctgtttc tttctcctta aattcaacat ctgctgggta agccacgctt
    18001 actttgctgc cacggaggtg tccgcctgca ggaaagcgca ccagacattt aatatgaccc
    18061 agaatgcgct tatcgtctgc tgcacaaact tggcctaact gctctaaata ttccgcaatt
    18121 agtgtctgcc aaacctctaa aggctgctcc tgctgaaggg accaactgat aatggctgca
    18181 taaggctcta gtcctagtcc ttccatggta aaatctcctt caatagctct ttggccagcc
    18241 cttggcgtgc ggaaaccaag tagcgcttaa cttccggatt gatttcctct actatacggt
    18301 gcacatcctg aagctgttcc gctgtggcca ggtctacctt attaacaagt agaatattgg
    18361 cgtttttaat ttgtctcgta attaacgggg tcacgatttc gtaaagctcg ggcaggcgta
    18421 aggaatcaag cacagctgta atgaaaacag actctaaagg ccgcccccta taatattgca
    18481 gtacattaag catactactt ggttccgctg tccctgaagg ctctatgaga accaaatcga
    18541 ctgcgtactc ctggttcagc ttttctagcg ttggcactaa atcactggcc agagtacagc
    18601 aaatgcaccc attggccagc tcataaacat tcaactccag ttctcgcatc agttcgccat
    18661 caatgccgac ctcgccaatc tcattaacta gaattgccat ttttcgtccc tgttgaatgc
    18721 attctgatgc cacctgaatg gctacagtgg tcttgcctga ccccagaaag cccgtaatca
    18781 tcagcaagtg catcgtccat ccctccctat aaaacatgat ttatgcaccc gctggggcaa
    18841 actagcgatt gcacaattga ctatatcatt tgccatcctc actgcgtaaa cattaggtca
    18901 tcagatactt acaaatagat tttctcccaa caaaattgac ataagtcgcc ggggcttaag
    18961 tttgaattat tttggattgt ttgatagtga tttaactggt tcgtacatag accctattgg
    19021 cattaaaatg ccaatagggt ctatttcctt atttaagcta tattagccct ctaatgttgc
    19081 agctataaca gctttaatat tatcaagagg ggcataagga ggcactgaac agccagatga
    19141 cataataaac ccgctcttgc caaatagttc gactaattta cgagcatagt tatagttatc
    19201 atccggagtg cccatttcta aaacagcagg agggacatct ccggtaacac acatgtaatc
    19261 gccaagaact tctttaattt taacaatatc cgttaagcta tccggatcaa agatacagga
    19321 gcccttggga aaatcacgcc aatatgacaa gtagttatcc cattttccat ccatatggaa
    19381 caaaaccttc gtgccggcag caataaaagc atctgctgtc tttttggtaa aacgccaggt
    19441 gaatttttca aaatttttca cattgaacag gtcatcgcca aaacggccat gccctgaaaa
    19501 gacagataat ggctgacgtt cacggattaa tttcgtggcc gattcgatat attcatcagc
    19561 aataatttcc attgcttcaa gaactttgtc cggcatacga tacagatctc ttaataattt
    19621 tttcaaggaa cgagctgcac tcagtatttc aacgggcata acatacgcac caccggacat
    19681 attaaccagt cccagctctt ctctgactac cttgttcatt ttatccatat attcaaaatc
    19741 cggagcaagc tgctcctcgg tgtagcccaa acgatccagc agttctttac ctaaggccgc
    19801 ccagcccttg ttaatgatcg tgtcataatc atcttctgtc ataggaccaa cttctacaat
    19861 ttgccaaagg gcatttgggg ggagctcttt gccaggaagt ttggtcttgg aataccacat
    19921 tgctcccatg ccttctctgc tgccaccaag cataggtcca gtatcaattt ccggcagcat
    19981 tttatatgaa cgtattacgg catcatctgc ccacttcggt cgcagaatgt aatcagataa
    20041 cgttgcttcg ggctccgaat aatagataaa acttggccct cctaaactaa tggggatacg
    20101 atcaggtgtc cccatatcca tagctgtttt aatccgctgc aagcgctgat tatagagctc
    20161 ttccacagtc attattattc ctcctaacct cttattcact aattaagcac atatttaccg
    20221 gtgatgaaat agcttaacaa ccgttttagc acttgtcaat ttgcggcgac caccgctaaa
    20281 tacatttaat aacaacccca tatctgcaca acggttcatt gataagcctt tccaggagca
    20341 gcaatcgttt cactaccccc tggaactatt gaatagtaag ctcatgttgt atcttaagct
    20401 atccactctt tgcagaaatt cactgcatca ctagcagtac cgaaagcatc tgcacccaca
    20461 aactggcaaa tatcagctgt gacaggagct ccaccgatca ttactttgac gtctaaaccg
    20521 gcggctttaa gcgcatccgc acactctctc attggcggga aagcaacggt taacagaccg
    20581 gaaatgccca caacaggtgc accggtttct tttactgctt caacaaaacg ctccaccggc
    20641 acatcaacac ctaaatcaat aacctcataa ccggcacttt gcaacaaaac gattacaata
    20701 tctttgccga tatcatgaat gtcaccaatg attgtcccaa ttacaacctt gcctttgggt
    20761 tcgcttcctg ctccttcagc aaaatatggt gttatcactt cactaactgc actgaaaact
    20821 tctccggaca taatcagatc agatagaaag tattcaccgc tttcaaaccg ttcaccgaca
    20881 accgccatgc cttgctggca agcgtcaaac agggctttgg cgtcttcacc agcatccaat
    20941 ctttctttga tccgctgcag taactgatct tcttcaagat ctgccattaa ggcaatgagt
    21001 tcttccatga ggatacctcc taatcattag tttactttac cctttctctg tatggcagat
    21061 ggctctgcct caaatgatgc gtacaacacc cggtatgcac caaccagacc tatgacacag
    21121 atacaataat cctccttcct attaggactc aataagattc aaaagctttt catagatgtt
    21181 aaagtattca caatgttaga tgcaataaaa cacgttttac gtgcttactc tggcatctag
    21241 ggctcccctc acaaatatta gcaggtgata cttttgttaa aaatatatgc cctttctgca
    21301 tgaatattca gactaatgtg gttatctttg tataggattc tacctctatt gtctaatgga
    21361 tttaattcgc aaatattctg aatacttaaa ttatagtaat tgtctccgtt ttttgtcaag
    21421 tactaaagta atcctaactt ttcgtgacac tatcacatag gtcagggtca gtgagacagg
    21481 taaaggtttt tatcccggct aaccacttga gtaccgccaa aacattcgct aatgagggga
    21541 catatcacca gaaggtccat cggcctaacc cagtgagcaa tgcgaacgga gttatcagga
    21601 tacaagtaac gctaaagagt aaaaaacaaa ctgccacaac cagcagcgtg gtcatctggt
    21661 tatgtagcat tcgcccaagg aggggacacg tccctgggag agtcacaggt gcgggtgcat
    21721 aaacaaacaa cgcaccgatt actcagtgcg ttgcgtaatg atagtctggc aaagacctac
    21781 tctcggccta acctagtgag caaagcgaac ggatgatact aagtcagcgt atcgctaaag
    21841 aggag
//
LOCUS       DASRMS010000032         1143 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_678740, whole genome shotgun sequence.
ACCESSION   DASRMS010000032 DASRMS010000000
VERSION     DASRMS010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678740"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VFC74_01285"
     CDS             complement(<1..510)
                     /locus_tag="VFC74_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24000.1"
                     /translation="MDLAICINTNSLPADTVERGKELMKDALAGVLQLIDSGNERVHF
                     YYDSNNEELFDLQLADNYSYEDFVADCGDVDLQAFLYEVEDKSPALDALTEAQLEEVS
                     EYDLYIPNQPLDAQRDIYSLAWVLDGYLLSLATTERWECFEVRANKQGNQGQYVDDFL
                     LLKNIANESH"
     gene            complement(514..>1143)
                     /locus_tag="VFC74_01290"
     CDS             complement(514..>1143)
                     /locus_tag="VFC74_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3696 domain-containing protein"
                     /protein_id="HZK24001.1"
                     /translation="GYVLPDELLHQNSKSKTFSHVLQDWLSEITPGVKFSYQRNTKAD
                     ISHAEIDSHRPTNVGFGLSYTLPIIAAALGSVAKPLSPEGNAPWVDTWESNKTNKGML
                     VMVENPEAHLHPKGQTAMGKLLALCAQQGAQVVVETHSEHVMDGIRIAIKQGQIAATG
                     VKFHYFTKNDQGRTELISPTIDENAKLDSWPEGFFDQTLKNRALLARRN"
BASE COUNT          289 a          271 c          229 g          354 t
ORIGIN      
        1 gtgtgactcg ttggctatat tttttaatag taaaaaatca tctacatact gaccctggtt
       61 accttgttta tttgcacgta cttcgaaaca ctcccagcgt tcggtggtgg ctaagcttag
      121 caaatagcca tctaacaccc atgcaagcga ataaatatca cgctgtgcat ctaaaggctg
      181 gttaggtata tacagatcgt attcagaaac ctcttctagc tgcgcttctg tcaaggcatc
      241 aagggccggg cttttatctt cgacctcata caaaaaagcc tgcaaatcta catcaccgca
      301 gtcagctaca aaatcttcgt aactgtagtt atcagcaagc tgtaaatcga acagctcttc
      361 gttgttggaa tcgtaataaa aatgcacacg ctcattaccg ctatctatga gctgtaatac
      421 accagccagc gcatctttca tcagctcttt gccccgctcc actgtatccg ccggcaaact
      481 attagtgttt atacagatgg ctaaatccat tttctagttc cttctagcga gtagcgccct
      541 attttttagg gtttgatcaa aaaaaccttc tggccaacta tctagcttag cgttttcatc
      601 aattgtgggg ctgatgagtt ctgttctgcc ttgatcattt ttagtgaagt aatggaattt
      661 aacacccgtt gcggctattt gaccttgttt aatagcaata cgaataccat ccataacatg
      721 ctcactgtgg gtttccacta ccacctgtgc gccttgctgt gcacataaag ctaaaagctt
      781 acccatggcc gtttgccctt tagggtgtag gtgcgcctct ggattttcta ccatcaccaa
      841 catgcctttg tttgtcttgt tagattccca agtatctacc caaggtgcat taccttctgg
      901 acttaatggt tttgctacac ttcctaaagc tgctgcaata atgggcagtg tgtaacttaa
      961 accaaaacct acgttggtgg ggcggtggct gtctatttct gcatgtgaaa tatccgcctt
     1021 ggtgttgcgt tggtagctaa actttacccc gggggtaatt tctgaaagcc agtcttgcag
     1081 cacgtggcta aaggttttgc ttttagagtt ttgatgaagc aactcatcag gcagcacata
     1141 acc
//
LOCUS       DASRMS010000033        49712 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_678769, whole genome shotgun sequence.
ACCESSION   DASRMS010000033 DASRMS010000000
VERSION     DASRMS010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 49712)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49712)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49712
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_678769"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(222..577)
                     /gene="ssrA"
                     /locus_tag="VFC74_01295"
     tmRNA           complement(222..577)
                     /gene="ssrA"
                     /locus_tag="VFC74_01295"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(585..1040)
                     /gene="smpB"
                     /locus_tag="VFC74_01300"
     CDS             complement(585..1040)
                     /gene="smpB"
                     /locus_tag="VFC74_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009623612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HZK24002.1"
                     /translation="MGDKVFAKNRKALHDYHLEETYEAGMVLTGTEVKSIRAGRINLK
                     DSYARIDSNEVWVHNMHISPYEQGNRFNHEPLRKRKLLMHRREINRLIGLTREKGYTL
                     IPTKVYSNKGYIKIEIALARGKKLYDKRASLKEKEAKREVERAFKESNQ"
     gene            complement(1218..1742)
                     /locus_tag="VFC74_01305"
                     /pseudo
     CDS             complement(1218..1742)
                     /locus_tag="VFC74_01305"
                     /inference="COORDINATES: protein motif:HMM:NF021252.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family (seleno)protein"
     gene            complement(1770..2018)
                     /locus_tag="VFC74_01310"
     CDS             complement(1770..2018)
                     /locus_tag="VFC74_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HZK24003.1"
                     /translation="MAVKEKELTRRKFIKGAGATLAVTALSAGLGGFLTGCSKPTEET
                     TGEPNGQPVDVTWPLAYHQLDSAKAEEIAYNSYKSGAG"
     gene            complement(2096..2269)
                     /gene="tatA"
                     /locus_tag="VFC74_01315"
     CDS             complement(2096..2269)
                     /gene="tatA"
                     /locus_tag="VFC74_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24004.1"
                     /translation="MSKIGLGELLLILVAVLLIFGPSKLPEVGKSLGKGIREFKNATK
                     EISDSIKDGSSEA"
     gene            complement(2482..3531)
                     /locus_tag="VFC74_01320"
     CDS             complement(2482..3531)
                     /locus_tag="VFC74_01320"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HZK24005.1"
                     /translation="MQMLYPIIFGLIFGYIIQRSRFCFAACFRDIFLIRNTAQTRAVL
                     VALFINTLSFALVHFFTAGSLGTAGRVYPVGVHTLVGGLFFGFGMVIAGSCLSGCLTR
                     IGEGNLMQIGTLFGLLLGSLLGAWHLNWWLPTIIASTPTIFLPDVVGWQAAQVLQLSI
                     ISLLYFTAAKYGGAKDLWSIFKQQGKIWPYGKGAVYLALANTMLFALWHRSWGISSGL
                     THLAGGLAAWLGLPVSSWQYFQHQLFRENTTAILLKHPIIYLALAMVLGSFVASVRHH
                     EFRLRKPRTNKYYYSALVGGIMMGYGSRLALGCNIGALLTGIASLSLHGWVFALAVFI
                     GAYGGSKALLRYLIT"
     gene            3749..4672
                     /locus_tag="VFC74_01325"
     CDS             3749..4672
                     /locus_tag="VFC74_01325"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZK24006.1"
                     /translation="MELDNLKAFCIAVESGSISKAAKKLYISQPSLSVKIQELERYYE
                     AILLNRTNKGIHPTDAGLIVYEHAQKMLSLSDSIERSLEQNQQDVQQLVVGTSSTIGN
                     FALPCTVYNFNEKYPHYKVSLIISNTEKVLENLATHKVDLGLIEGPLTKELKHTLTQE
                     GIKTKHVISNPLILVVPNDENWQNINSIHRDQISQLPLIMREEGSGIHSTLKLGLAKL
                     NLAFDDLNVVMQLNSTNAIISAVASGKGVSFLPQVALRKELHYKICKEVKVQDVVFRH
                     SFTTLYYPNDNQHSLQTAFLGFLHSSERGFC"
     gene            complement(4669..4896)
                     /locus_tag="VFC74_01330"
     CDS             complement(4669..4896)
                     /locus_tag="VFC74_01330"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZK24007.1"
                     /translation="MDHNIDAIGEFCPIPIIRAEVKLTQIKPGDRVIITTDHTCSSAS
                     VVGHFESKYKYPCSLTQVGEGIWEITILKKD"
     gene            complement(5192..7312)
                     /gene="rnr"
                     /locus_tag="VFC74_01335"
     CDS             complement(5192..7312)
                     /gene="rnr"
                     /locus_tag="VFC74_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HZK24008.1"
                     /translation="MSFQDKILAYMREVAYKPLKTEELTQELGINEKKDIKHFNTLLV
                     EMEQEGQVIKTRYGRYGVPEKMNLIVGRLQGNQAGFGFIIPDDTTHADVFVPASEMNG
                     AMHGDRVVARLGKGGSGRKQEGEIIRILKRRSSLIVGRYEAGRQFGFVVPDDQRISQD
                     IFLPKAEAKGLKNGMKIQIEITRWPEKRRNPEGKVVDILGFPGDKGVDTLTIIKKYEL
                     PEDFSREIKKELQSFSGEVSEEEIAGRRDLRAQQLVTIDGADAKDLDDAVSLQRQPNG
                     NWLLGVHIADVSYYVKEGSLLDREALARGTSVYLVDRVIPMLPPELSNDLCSLNAQVD
                     RLAMSVLMEVDAQGKVVTHEFTPSVIRTVERMTYDAVRAILEEQDDEQRQRYTKLVPM
                     FEEMGELALVLRNKRIARGAIDFALPEVKVLLDDDGKPVDIVKRERTIAETIIEEFML
                     ICNETVAEHFARLELPFVYRIHENPDEAKMLHFRDFAHNLGISIKGNPEKIHPRVLQE
                     ILKEVAGKPEERVINTLLLRAMKQAKYAPDRLAHYGLAAEYYSHFTSPIRRYPDLVIH
                     RLMREYLTKVPPQKRLASIYKSNTVAAERSSLRERVAMEAERESVEVKMIEFMADKVG
                     QEFDAVISGVTSFGLFAELDNLVEGLIHVSSMDDDYYHYHEEKLALIGERTGNTFRLG
                     EPVRVILKRASKEDRQLDFALAPA"
     gene            complement(7497..9494)
                     /locus_tag="VFC74_01340"
     CDS             complement(7497..9494)
                     /locus_tag="VFC74_01340"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005341143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZK24009.1"
                     /translation="MDNILYLIPAAGLVGLLFAFYLANKVSSADPGNDRMQEIADEIH
                     KGAMAFLTREYRTLAIFVIILFAVITFAIDLQTAICFLCGSLASAIAGYIGMNIATKA
                     NVRTTNAAQHGTNPALTIAFSGGAVMGLSVVGLGLLGLGLLYLIYKDPEIVNGYALGA
                     SSIALFARVGGGIYTKAADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDVAGMG
                     ADLFESYVGSIIAAMTIGIATFGIEGALVPMIVAASGVFAAIIGTFFVKAKEGVKLSS
                     SLERGTLVSGILVVITSFFVIRYYSFEFGGIGPFWAVVAGLVAGIIIGRITEYYTSDH
                     YRPVQDIADSSETGPATNIISGLAVGMRSTMLPLLVICLAILVANQSAGLYGIAISAV
                     GMLATAGMTIAVDAYGPIADNAGGIAEMAGLDESVRKITDELDSIGNTTAAIGKGFAI
                     GSAALTALALFSAFAQAAGIESIDITNAYVIVGLLIGGMLPFYFSALTMEAVGSAAFE
                     MIEEVRRQFREIPGLMEGTAKPDSARCVDISTGAALRQMVLPGLMAVVVPLLVGVVLG
                     KEALGGLLAGSLVTGVLMAIFMSNAGGAWDNAKKYIEGGAHGGKGSEPHKAAVVGDTV
                     GDPFKDTSGPSLNILIKLMSIVALVFAPLFLRIVPLIELIF"
     gene            complement(9601..9825)
                     /gene="secG"
                     /locus_tag="VFC74_01345"
     CDS             complement(9601..9825)
                     /gene="secG"
                     /locus_tag="VFC74_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HZK24010.1"
                     /translation="MEFVLNLIFLIAGLGMIASVLLQSSKSAGLSGSITGGAQTLFGK
                     KKGLDDFLAKLTIIFAVLFMGLAIILTSLS"
     gene            complement(9913..11190)
                     /gene="eno"
                     /locus_tag="VFC74_01350"
     CDS             complement(9913..11190)
                     /gene="eno"
                     /locus_tag="VFC74_01350"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HZK24011.1"
                     /translation="MTQIVEILAREILDSRGNPTVEVDVWLASGAFGRAAVPSGASTG
                     AFEAVELRDGDPARYLGKGVQQAVRHVNDIIAPAVLGMDALKQAELDQTLIALDGTLN
                     KGKLGANAILGVSMATAKAAANALGLPLYRYLGGANAKLLPVPMMNILNGGQHADNNV
                     DIQEFMVMPVGAATFKEGLRMGTEVFHHLRKVLQQHGLNTAVGDEGGFAPNLSSNEAA
                     LEEIVKAITAAGYQPGVDVVLALDVAATEMYKDGFYHLAGEGLQKTAAEMVDYYQRLV
                     DTYPVVSIEDGLAEDDWEGWQLLTDRLGQKVQLVGDDLFVTNIERLQMGIDRATGNAI
                     LIKLNQIGTVTETLDCIQLAQQHGYNAVISHRSGETDDVTIADLAVATAAGQIKTGAP
                     SRVDRVAKYNQLLRIEEQVADSALYAGNPFKNA"
     gene            complement(11213..12760)
                     /gene="gpmI"
                     /locus_tag="VFC74_01355"
     CDS             complement(11213..12760)
                     /gene="gpmI"
                     /locus_tag="VFC74_01355"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HZK24012.1"
                     /translation="MSRPRPLALIILDGWGLGAEDEGNAICQAQTPFFDSLWATYPTT
                     SLMASGQAVGLPAGQMGNSEVGHLNIGAGRIVYQDLSRISKAISEGSFCENEKLLTVM
                     QQVRESDSALHLMGLLSDGGVHSHIQHLFALLEMAKREQVKKVFIHAILDGRDVGPTS
                     ARQYLAALEEKMAQLGVGAVATVSGRYYTMDRDQRWERVKRAYRAMVYGEGPVAACAL
                     HALESAYDREETDEFVSPTVILQGEHPVSTINALDSIIFFNFRPDRARQITRTFTEEN
                     FSCFDRGQAPPFPLFTCMTQYAEDIKVPVAFPPEHPRQTLGEVVAAAGLRQLRIAETE
                     KYAHVTFFFSGGEETPFAGEERILIPSPKVPTYNLQPEMSALQVTARVIEEIKRGVHD
                     LIVLNYANSDMVGHTGVLEATVCAVEKVDDYLREVVEAVLAAGGAALITADHGNAEQM
                     TAGQTEHPFTAHTANPVPFIYVSTTPRRLHSQGILADVAPTILELLALPKPAVMTGRS
                     LLLPADN"
     gene            complement(12760..13524)
                     /gene="tpiA"
                     /locus_tag="VFC74_01360"
     CDS             complement(12760..13524)
                     /gene="tpiA"
                     /locus_tag="VFC74_01360"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HZK24013.1"
                     /translation="MRVPIIAANWKMHKTVREAVDYVEQFAGLLEKGNVETVICPPFP
                     LLGLINEACHQSGLRLGAQNMYWEETGAFTGEVSPLLLKDLAVDYVILGHSERRQLFG
                     ETDQQVARKVKAAFAHELTPILCVGETLQQRQAEQTMPVIAEQVSVGLAGLDPSQVRR
                     VVVAYEPVWAIGSGLAATGDDAKEVALHIRNTITEQFSLKVARDVRIQYGGSVKPNNI
                     LDFMQEIEIDGALVGGAGLDAAVFADLINRAQGESV"
     gene            complement(13526..14719)
                     /locus_tag="VFC74_01365"
     CDS             complement(13526..14719)
                     /locus_tag="VFC74_01365"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HZK24014.1"
                     /translation="MTIKTVKDVAWAGKRVFTRVDFNVPRTEQGAVADDTKIRAALPT
                     ISYLLQQGAAVILASHLGRPKGQVKEELRLTAVAKRLEELLKVKVVKTDDVVGPEVER
                     LAQQLQPGQVMLLENVRFHPGEEKNERELAQAYAKLADVFVSDAFGTVHRAHASNVGV
                     AEFLPAAAGFLMQKEVETLTKTLHNAQKPVLAIVGGSKTADKIGVIKAFLKLAETILL
                     GGGMANTFLKAQGCQMGKSLVEDDQVENASQIMREAKALQVELCLPVDVTIAAGIDDA
                     AGAQTVKAEAVPAAKMVLDIGPETRRLYAERIKQAGTVIWNGPLGVFEVDTFAQGTIE
                     IAQAVAGASAFSLIGGGDVVAAVEKAGVADKISHLSTGGGAALEFWEGKELPGIVVLK
                     QTEEV"
     gene            complement(14811..15815)
                     /gene="gap"
                     /locus_tag="VFC74_01370"
     CDS             complement(14811..15815)
                     /gene="gap"
                     /locus_tag="VFC74_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZK24015.1"
                     /translation="MTIRVGVNGFGRIGKACVRLAHENPELELVAFNSTRDPKTLAHL
                     LKYDSLYGRVPFTVEATDGALLLDGKEVKILAERDPAKLDWNALDVDIVIEATGAFRS
                     AAEAGKHLGGSVKKVIITAPAKEEDLTVVMGVNHDAYDPAQHHIISNASCTTNCLAPL
                     CKVLDEEFGFIRGLMTTIHAYTNDQKILDVAHSDLRRARAAGLSMIPTTTGAARAVSL
                     VLPGIQGKIDGFAVRIPTPTVSMVDLTAELNKNVTAAEVNEAFRRAAEGAMAGILGYS
                     EEPLVSVDYQGDSHSSTVDALSTLVVKDNLVKVVSWYDNEWGYACRVIDLAAYIGKQF
                     "
     gene            complement(16136..17521)
                     /gene="rpoN"
                     /locus_tag="VFC74_01375"
     CDS             complement(16136..17521)
                     /gene="rpoN"
                     /locus_tag="VFC74_01375"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HZK24016.1"
                     /translation="MHITHNLKLEQTQKLIMTPELQQAISLLQLPVMELQAFLQNEYL
                     NNPVLDLDEEEDKKAEEVQVDSKLENDSIDWDEYLRDQGYEPLPAVQSRANDAAAAVD
                     YALSAEPSLQEHLSSQLGFCSLTTTEKRIGEFLIGNIDSNGYVNGKMEELAALSGLAC
                     SELTAILKVIQTFDPVGVGARDLQECLLLQLQELPDAHPLAEAIVSQHLEDVADNKQK
                     KIAAALQVEPAAVQAAIDFIRTLDPKPGRLIGGAADVRYVVPDVSVEKVQDKYVIIVN
                     EQNMPRLTINPYYRSLLGNENQVASDFLKSRFDAALWLVRSLEQRRLTLYRVTECLVK
                     IQQAFLDEGIKYLKPLTLRQIAEEIGVHESTVSRTTANKYLQTPRGLYAFKFFFASGV
                     EDYHGTAISSASVKSHMKELFAAEDQYKPYSDKKLGELLAARGIVVSRRTVAKYREEL
                     GVPASSKRKRV"
     gene            complement(17730..18665)
                     /gene="whiA"
                     /locus_tag="VFC74_01380"
     CDS             complement(17730..18665)
                     /gene="whiA"
                     /locus_tag="VFC74_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0043937 - regulation of sporulation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HZK24017.1"
                     /translation="MTYAMQAKNELARRELGGACCQAAELTAFIRHSGNIQISEKRLA
                     LHVVTSNPAVARRIFSILKQTFKFNSELLVRRKIRLRKNNVYLIRIKEKEHVLKILQA
                     LGLLIDGDLKQEISEKVRKKKCCRRAYLRGAFLASGSVTNPENSYHLEIFTAYQQQAD
                     DLVSLMASFQVTAKTISRKNGWLVYLKDSEQIVEFLNVIGAHNALLNFENVRILKDLR
                     NRVNRLINFETANMNKTVDASVRQLEMIRLIDEKVGLEALDAPLNEVARLRLAHPEAS
                     LQELGDLLEPPLSKSGVNHRMRKLETLSKQLAKDS"
     gene            complement(18723..19706)
                     /locus_tag="VFC74_01385"
     CDS             complement(18723..19706)
                     /locus_tag="VFC74_01385"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HZK24018.1"
                     /translation="MKIEATASNHYERGKELMVAALGGGTGLSTLLRGLKNYTTKLAA
                     IVSVADDGGSSGRLRQNLGILPPGDIRNCLLALANTEPLLEQVFQHRFLSGEGLAGHN
                     FGNLFLAALTEEFGFEKAIRVASRVLAVTGEVLPVSMAKLNLVAELTDGRTIRGQSRI
                     PQAGGIIRRLYIEPATADIYPAARKSILAAQLVVVGPGSLYTSVLANLLVPGVVQALR
                     DTAAHKVYVCNVMTQPGETEKFSAADHFQVLTEHVGTGVFDTILINNNLHIPAALLQK
                     YAAEGAIPVQPDLKRLQTMGVRVVAADLLSPVALVRHDPDKLACAIFTDVL"
     gene            complement(19733..20590)
                     /gene="rapZ"
                     /locus_tag="VFC74_01390"
     CDS             complement(19733..20590)
                     /gene="rapZ"
                     /locus_tag="VFC74_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HZK24019.1"
                     /translation="MRFVIITGQSGAGKSQAMHCFEDLGYFCIDNLPPMLIPKFAELA
                     VQSEGKINRIALVSDIRGGDFFASLQEALQMLEGMGTDYEVLFLEASDEVLIRRFKET
                     RRRHPLATMGSIAEGIKEEKKLLAQIRAHADKLIDTSYLTPHELKEEITTLYAPDSKQ
                     ENILITLVAFGFKFGIPLDADLVFDVRFLPNPHYVENLRPLTGNHELVKNYVWKWSIT
                     YKFFRKLLDLIEFLIPCYIKEGKPQLVIAIGCTGGKHRSVSIVNELGKILQGKNYPVV
                     KEYRDIDKV"
     gene            complement(20639..21400)
                     /locus_tag="VFC74_01395"
     CDS             complement(20639..21400)
                     /locus_tag="VFC74_01395"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZK24020.1"
                     /translation="MQATADFHTHTIYSHGKGTIKENVQAAQRRGLKTIGIADHGPGH
                     FFIGVRGADTFRRMREEINSLRLEYPELEILLGVEANIVDVDGTIDVPESILSELDYL
                     LVGYHKLVRPRSWSAFWLGAQNFLAGWLGLTVPRLRQQNTAAICAAVRRYPVYAITHP
                     GLQIDIDTHALAEACAETETLMEINSSYAPKLAGYVQVALPLGVKFVVNSDAHIPARV
                     GDFTAAYQLIERLNLPLKRLVNIAPAQAEKQELPL"
     gene            complement(21584..22960)
                     /locus_tag="VFC74_01400"
     CDS             complement(21584..22960)
                     /locus_tag="VFC74_01400"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24021.1"
                     /translation="MSGGRLTNAEVNTYGSYALFSGLGIAIPMSYGVLFMTDHLMISA
                     AVVATLLLVARILDFAVCLASGAIVQKSNLKWGKYRSWFVILRWVVVVGISLQFINTS
                     SLAMPLRLAIVTVGYFMLHFSMNFVALSQFGILASMAGTSMIDRNRLSARQAQFQTAG
                     AIITSASIIPLLNFFTPIVGASNAYIAVAGPFALIYAIGAGIILKVSAPYDRPEDAKR
                     GGPVVTVKDMINSVVTNSQLLIYVLVMSLTQVGAMGAMGIQTYYFIYVLGNFNLMAVA
                     MTTMTMVSFFAAMVGPSIGKKLGKKMALVTGLIMMAACSLCITLFARESIVIYIVFAA
                     LRQASMYLYMTFGPNYSIDAAEYGLYTTGKDNRTVAMSMNNIPIKVGFALGGAIGTFG
                     LSIIGYTAGMTATPEFVNGFMWIFGGIPAGFYLLASMIMFFGYKITDEDAAMYARENA
                     ARLAAQKG"
     gene            complement(23407..24783)
                     /locus_tag="VFC74_01405"
     CDS             complement(23407..24783)
                     /locus_tag="VFC74_01405"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24022.1"
                     /translation="MSGGKLTTKEINMYGAYALASGIGIAIPMSYGVLFMTDHLMIST
                     SIVATLLLVARILDFGVSLTAGAVVQKSNLKWGKYRSWLLILRWVVFTGICLQFINTT
                     SLPMPVRLAIVVIGYFMLHFSMNYIALSQFGVLASMAGTSMEDRTRLSSRQAQFQTAG
                     AIITSASIIPLLNFFKPIVGAANAYIVVAAPFAFVLVIGAGLITKAAEPYDRPEAAKR
                     GGPVVTVKDMINSVVTNSQLLIYVLVMSLTQVGAMGAMGIQTYYFIYVLGNFNLMAVA
                     MTTMTVFSFFAATVGPSIGRKLGKKMALVTGLMIMAASSVCITIFARNSVVTYIIFAC
                     LRQGAMYMYMCFGPNYAIDAAEYGLYATGKDNRTVAMSMNNIPIKVGFALGGAIGTFG
                     LSIIGYTAGMTATPEFITGFMWIFGGIPAGFYLLASLIMLLGYKITDEDAAMYARENA
                     AKLAAQKA"
     gene            complement(25251..25931)
                     /locus_tag="VFC74_01410"
     CDS             complement(25251..25931)
                     /locus_tag="VFC74_01410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZK24023.1"
                     /translation="MQRSAKYLQKVPFFAGLAEKELEIVSSVMIERNYAKGAVLFLEG
                     ERGESLFVINHGRVKISKSSPDGREQILHIYNDGDIFAEVVLFDRGPYPATAEAVEDT
                     QCWLLRNADMERLLAAQPQLGIKLLRIMSRRLRRAQLLVRDLALHDTYARLAGLLLRF
                     VRLEGRPQEDGVVLDLDLTRQEMASMIGTSRETVARILSRFQKEGIIVLDKQRIIIKD
                     QAKLHDWT"
     gene            complement(25961..26971)
                     /locus_tag="VFC74_01415"
     CDS             complement(25961..26971)
                     /locus_tag="VFC74_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HZK24024.1"
                     /translation="MNVQEIRERARGRLRGSCRVCRVCDGRACAGEVPGIGGLLTGSS
                     FQNNVESLAMYHLNMRTIHQVDQPDLTLKLFGRNFATPVLVAPLTGVAYNLGGALSEE
                     EFVTHLVEGAKAAGSCAFTGDGADPQFYRAGLEAIEKADNGIAIIKPRAMEPLKERIR
                     QAEASGALAVGIDLDGAGLVTMAMKGQPVGPKSFYQLRELVKITKLPFILKGIMTAQE
                     AEMAVEIGAAAIVISNHGGRVLDGTPGVAEVLPEIVERVKGEIIIFADGGIRNGIDAL
                     KMLALGANAVLIGRPAIWGAFGAGTEGVNKIIETMTEHLRHGMLMTGCVNLKAVDYHI
                     IY"
     gene            complement(27206..27550)
                     /locus_tag="VFC74_01420"
     CDS             complement(27206..27550)
                     /locus_tag="VFC74_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZK24025.1"
                     /translation="MRSLVWRWVLNALALYLTSLVINGVDIESFAAALVAALVWGFVN
                     TIIRPLINLITLPVNIMTVGLFTFVINGSLLWLVADTVKGFVVQSLWAGILGALLLSI
                     ISSVLSALLADN"
     gene            complement(27665..29536)
                     /gene="uvrC"
                     /locus_tag="VFC74_01425"
     CDS             complement(27665..29536)
                     /gene="uvrC"
                     /locus_tag="VFC74_01425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HZK24026.1"
                     /translation="MSEVKQLLAEKLKQLPRRPGVYLMKDERGKIIYIGKAIDLRQRV
                     RSYFQAAHNLTPKVRALVEKICDLDYTVTDTEVEALILESNLIKEYRPRYNIDLKDDK
                     HYPYLRVTVQEKFPRIHVARRVEKDGARYFGPYPNAGAVKETIRLLHRLFPLRHCRGP
                     LNAERAKRPCLNRHIGRCLAPCAGTVSLEEYRSLVDEVLLFLEGKEDKLLRDLVQRMA
                     EAAEKLDFEKAARLRDQYRAVKAISEKQKIAIAAGGDRDIIALAAGEDSGCAQLFAVR
                     NGKLTGREHFFLTNPTGEAAPELLTAFLHRYYTHAGDLPAEILLSEEVSEVELLAAWL
                     SQKRGTKVRLHVPQRGAKKELMRLALDNAHLFLEQEVLRVKARDPQVALQALAEAVDL
                     ESPPLRIEGYDISHFQGEGTVASMVVFENGLAQTAEYRRFRIRSVDKPDDYASMKEVI
                     NRRFARLKAARAAGDLPVTTSDPFLLVPDLILIDGGRGQLNVALQALTEQDYALLPVI
                     ALSEEEELLHLPDQPQPLRLPRTSPALQLLQRVRDESHRYAITQQRKQLNRPARGSLL
                     LEVPGIGEKRRKALLQAFGSIKALREATVEELAAVAGMNQAVAATLYAYFQEDADRK"
     gene            complement(29549..32368)
                     /gene="uvrA"
                     /locus_tag="VFC74_01430"
     CDS             complement(29549..32368)
                     /gene="uvrA"
                     /locus_tag="VFC74_01430"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HZK24027.1"
                     /translation="MKQDTISIRGAREHNLKNINVDIPRNQLVVITGVSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDYIEGLSPAISIDQKTTSNNPRSTVGT
                     VTEIYDYLRLLFARIGHPSCPHCDLPIAQQTVEQMVDRIMALPDKTRIQVLAPLVRGR
                     KGEHRKLFQDVRADGFVRVRVDGELRELEEEIELVKNKKHHIEIVVDRLIIKKGLEHR
                     LADSLETALKYGNGLVLIDVIEQEELLFSSAFACPQCGFSFEELTPRMFSFNNPYGAC
                     PACSGLGSTSEFDPELVIPDPQRSLREGAVVPWKPGTYYYQLLLAMAQHHEIDVDKPF
                     ALLSKGQQKKVLWGIRERLPFDYEESSGRIQHYELKYNGVIPTLTRRYKETTSEQYRE
                     HLATFLSEHLCSECHGMRLKPATLAVTVGGKNIAQVTSLTVDKAKDFVASLQLSAKEE
                     QIARLIVKELQARLNFLLNVGLSYLSLNRAAGSLSGGEAQRIRLATQIGSSLMGVLYI
                     LDEPSIGLHQRDNARLIETLERLRDLGNTVIVVEHDEETISRADHIIDVGPGAGVWGG
                     NIVAAGTAADLKEVPASITGQYLSGVKTIRVPAERRPLNGKWLEIKGARAHNLKNLDV
                     RVPLGVFTCVTGVSGSGKSTLVNEILYRRLAMELHRNTHKPAEHDDLLGLEYLDKVID
                     VDQSPIGRTPRSNAATYTGVFDYIRELFSRTNESRARGYKPGRFSFNVKGGRCEACRG
                     DGILRIEMHFLPDVYVPCEVCHGKRYNRETLEVRYKGKSIADVLEMTVAEAVEFFDPI
                     PRIKRKMQTLYDVGLGYIRLGQPAPTLSGGEAQRVKLATELSRRSNGKTLYILDEPTT
                     GLHSDDINKLLTILDRLVENGDSVLVIEHNLDVIKRADYLIDLGPEGGDAGGQVIASG
                     TPEEVILVPASYTGRYLKKYLVPAH"
     gene            complement(32369..34366)
                     /gene="uvrB"
                     /locus_tag="VFC74_01435"
     CDS             complement(32369..34366)
                     /gene="uvrB"
                     /locus_tag="VFC74_01435"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HZK24028.1"
                     /translation="MGKFQLQSSYRPEGDQPQAIRKLVAGLESGWKHQTLLGVTGSGK
                     TFTMANIVEEVQRPTLVIAHNKTLAAQLCSEFREFFPDNAVEYFVSYYDYYQPEAYIP
                     YTDTYIEKDSSINEEIEKLRHSATSALFERRDVLIVASVSSIYGLGSPDEYSAHMVSL
                     RVGMEKSREQVVRELIANQYERNDLQLERSRFRVRGDVLEIYPASATEQAVRIEFFGD
                     VIERIREIDTLTSEVLTERNHITIFPASHYVTSRDVLEEAMVDIRLELEERLQEFRAT
                     GKLLEAQRLEQRTKYDLEMMNELGFCTGIENYSRHMDRRTAGTRPFTLIDYFPQDFLL
                     LIDESHQTIPQIRGMYAGDRSRKQVLVDYGFRLPSALDNRPLMFPEFEELVCQAVYVS
                     ATPGPYEQEHSQQVVEQIIRPTGLLDPAVIVRPVKGQIDDLYGEIRQRVEKNERVLIT
                     TLTKRMSEDLTEYYKELGLKVRYLHSEIHTLERMEIIRDLRLGEFDVLVGINLLREGL
                     DLPEVSLVAILDADKEGFLRAHRSLIQTIGRAARNVDGKVIMYADKITDSMRLAIDET
                     ERRRTIQAEYNEKHGITPVSIQKEVRGVIEASKTAEEVPRYAATARAAGEMSAAERKK
                     EIQRLGKEMKQAAKDLEFERAAQLRDLIFKLRKAGKEDGLE"
     gene            complement(34651..34938)
                     /locus_tag="VFC74_01440"
     CDS             complement(34651..34938)
                     /locus_tag="VFC74_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24029.1"
                     /translation="MKEMQQNLQEKEFLLKQLCRRQEERDRQALQGLQQAMNALLLVQ
                     QQLTADLKFHQNLRQSQDFAQLKNTTLPDGSAAENYGQLPPWIFSQPPELQ"
     gene            complement(35019..36443)
                     /locus_tag="VFC74_01445"
     CDS             complement(35019..36443)
                     /locus_tag="VFC74_01445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HZK24030.1"
                     /translation="MKRRIVLLLVLLLVVTNSLTYFGTRDHYQQETAGEPPADNSRDL
                     AEIEVELQPLVEVLDILSERFLEDVSREELINEAINGMVNSLEDPQTNFLDPTNYEEM
                     MIKIDGSFSGIGIEISEVDGYITVVSPIKNTPGEQAGLQPGDRIVAIDGEDAVGISTT
                     DAVKLMRGQEGTTVNLKVERENLTEPLEFVITRANIVVPSVFTELLSDKIGYIQITTF
                     DEHTGRDFQEAMLALETQGMRGLLLDLRNNPGGLLDQAVLVGQEILPAGPITHMVDRE
                     GTVLQTYQSYGTKKTYPIVALVNGASASASEIIAGALQDTAAGTLVGTKTYGKATVQH
                     LESLSNQAGLRYTVAKYQTPKGRDIHGNGLVPDVIVELSDDYYLLQYGMIGDLALSDE
                     GTNVLFLQKMLAALNYAVQETSKFDGETEKAVRSFQSKYGLEVNGKVNQATREKLNEQ
                     LEPRLEELDVQKKKALEILTKAMQ"
     gene            complement(36709..37896)
                     /locus_tag="VFC74_01450"
     CDS             complement(36709..37896)
                     /locus_tag="VFC74_01450"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HZK24031.1"
                     /translation="MFKRKYAVLLLLVVAMLLSSMLPVLANTVDEKQDELEGVQENID
                     QRQEELQQNKQEQNKVKQEIELIQREVEVIEAELRAIGTKIRRTEEEITAVEAHLAEA
                     EEKLAEIDEVLAVRLRALHEHGQVSYLEVLFSSTSFADFLTRYNDLRQLIDQDKQLLE
                     EAMAERDRVAELKESLEKRRQELLTLRRNNINKKQEQEVKQARQKLLVSDLQAAYNKT
                     NNEIRKLEAEAQKIENLILQMQAQQNGSANQGTGTLGWPVQGYGRGWITSGYGYRRDP
                     ISGKQGSFHGGIDIGIPRNRWPGAGNYNGNPVNLLAADAGVAHTYRMGSGYGNLVIVD
                     HGKGVATVYGHTHDFLVANGQAVYKGQAVAIVGSTGYSTGPHVHFEVRINGERVNPLN
                     YVH"
     gene            complement(37913..38797)
                     /gene="ftsX"
                     /locus_tag="VFC74_01455"
     CDS             complement(37913..38797)
                     /gene="ftsX"
                     /locus_tag="VFC74_01455"
                     /inference="COORDINATES: protein motif:HMM:NF038347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZK24032.1"
                     /translation="MLIRNIKYYFQEAFRSIFRNGWMSFASVAVVTVTLLILGSFMLL
                     NLNVEHITQDIKDQVEIIAYVDEELAPSEVEELRVTVIQLSNVEEVRYVTKDQALVRL
                     QNQLGELVAGYEEEGRNPLRDSFEIRTKVPEDIPTVAKSLQTLPGIARVDYGTDVVDK
                     LFRFTGAVRWVGLAFMGGLAFTAMFLIANTIKLTVNARSKEIMIMKWVGATEWFIRWP
                     FVIEGIVLGTVGALLPALILIYVYREVVAWTQMNLFFLPLLPPQMILASITKLLLLLG
                     ITIGGLGSLLSMRRFLKV"
     gene            complement(38787..39476)
                     /gene="ftsE"
                     /locus_tag="VFC74_01460"
     CDS             complement(38787..39476)
                     /gene="ftsE"
                     /locus_tag="VFC74_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007787528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZK24033.1"
                     /translation="MIVMQHVNMVYPNSVGALNDINFSVERGEFVFIVGTSGAGKSTL
                     IRLIGREFLPTSGSIRVFSKEITRLRRREVSMFRRSIGVVFQDFRLLDKRTIYDNVAF
                     AMQVVGATPREVRRRVPEALEMVGLTGKAKRKPHELSGGEQQRVCLARAIINQPSLVI
                     ADEPTGNLDPQTSWGIVDLLKTINIRGTTIIMATHDKEIVDRMKQRVVVLEDGQIVHD
                     EQKGVYNHHAH"
     gene            complement(39842..40690)
                     /locus_tag="VFC74_01465"
     CDS             complement(39842..40690)
                     /locus_tag="VFC74_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HZK24034.1"
                     /translation="MAKWQLLKEYIGVLMGTAVIAAAFNMFMTPNQIAAGGISGLGVL
                     LYYLFNLPVGITIFLLNIPLFAIAVKLFGWHYITRGLFGAVALSMMIEVLTFLPVVTE
                     DLLLASLYGGIVMGIGVALVFRAHGTTGGTDLAAQVLNKIFGFSLGQSLLGVDFLVVA
                     LAGIVFNLELSMYALISLFVTSRVIDFVQEGLSSSKAALIISSETESMVARILREMNR
                     GATILTGEGAFTGQKRRMVLTVVAQSEVPRLKTIVHEIDAKAFVILGNAHEVLGEGFS
                     PTRGQA"
     gene            complement(40708..41818)
                     /gene="prfB"
                     /locus_tag="VFC74_01470"
     CDS             complement(join(40708..41748,41750..41818))
                     /gene="prfB"
                     /locus_tag="VFC74_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843576.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HZK24035.1"
                     /translation="MSAEIKACYQQLSERLTEMSVSLDLVGQQEQIKKLDLQVSQSDF
                     WSNSEQAQKIMQELNARRDEIGPLEAAQRQLDDLQLTITLAEDEDDDELWAEAAAGIT
                     ELQKELGKLELAVIFRGRYDHNSAIIAIHPGAGGTESQDWAAMLLRMYTRWAERKSYQ
                     VEVLDLLPGDEAGIKSATLLVTGKNAYGYLKAERGVHRLVRISPFDAASRRHTSFASL
                     DVLPDVEDTDQDIELRPEDLKIDTYRASGAGGQHVNTTDSAVRITHLPTGFTVQCQNE
                     RSQHSNRERALTILRARLADYYERERLKKISNLRGEQKEIGWGSQIRSYVFHPYTLVK
                     DHRTGVDVGNVNAVMDGELDQLIESYLRQTAGKDV"
     gene            complement(41827..44682)
                     /gene="secA"
                     /locus_tag="VFC74_01475"
     CDS             complement(41827..44682)
                     /gene="secA"
                     /locus_tag="VFC74_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HZK24036.1"
                     /translation="MRGLLARLIGDVNQREIKKVKPVVENINRFKEQTQALSDEALRN
                     KTDEFRKRFQQTVLEAQRTVNEIKSHLPENPDSFARRELAEAEQQLFQSEQAALWELL
                     PEAFAVVREASWRVLHMSHFDVQLLGGIILHQGRIAEMKTGEGKTLVATLPAYLNALA
                     GHGVHIVTVNDYLASRDKEWMGPLYEFLGLSVGLIVHGLSPLERRDAYHADITYGTNN
                     EFGFDYLRDNMVRYQEDLVQRPMSYAIVDEVDSILIDEARTPLIISGQMKTDEQQHQD
                     YERVEQAVRRLKAESHYTIDEKAKSALLTEEGEYQIEALLGVKGLYGEEYLSFDELEA
                     EQEDSDSQEIEDKNFLKKKVENALRAHYLMKRDRDYVVKDGQIIIVDEFTGRLMYGRR
                     YSEGLHQAIEAKERVKVAKESMTLASITFQNYFRMYKKLAGMTGTAATEETEFQSIYG
                     MDVVVTPTNQPMIRHDLPDVIYKTINGKFRWVVEEIVARHQTGQPLLVGTISIEISEA
                     LSRLLQKRGVRHEVLNAKYHEKEAKIIAQAGRLGAVTIATNMAGRGTDILLGGNPEAL
                     ANDDFRAQFGCELEQYVQQTAVEESDKAAAKERYQQLLAAYEEQSKQEREQVLAVGGL
                     HILGTERHESRRIDNQLRGRAGRQGDPGSSQFYISLEDDLMRLFGGENIFGVMEKLGL
                     DEDTPIDHPLISRALESAQKKVENRNFETRKHILEYDNVINEQREVIYSQRRRVLEGE
                     SLRETVLEWLPELLTNAMDQYAPAGVFAEEWDGRGLLAWAEALFLPPRRWTAAALAEN
                     DRDELFALLLEAAVATYTAREAKLGAEKMRELERVVILRAVDAKWMIHIDAMDMLRHE
                     IGLRAYGQRDPLVEYKYEAKNMFDQMIAEIQEESVALLYQVQLVEPPQRQAVAVTNET
                     PPSQGTAVHGPASAKKVGRNDPCPCGSGKKYKKCCGK"
     gene            complement(44792..45307)
                     /locus_tag="VFC74_01480"
     CDS             complement(44792..45307)
                     /locus_tag="VFC74_01480"
                     /inference="COORDINATES: protein motif:HMM:NF036410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HZK24037.1"
                     /translation="MIAVGVVLAVIMAKLRGGKLERLHDLGLKGLPWVILALLLRLLV
                     RVLAVRAIDMAWLQIVAYLLLFYALLQNLQLPGLKLFTAGSCLNFLVIAINGGTMPVS
                     AVAMSFANLQGDPAGTHNLLTATTRLFFLADIIPLRPPYFPVAEVISMGDIFIVAGIF
                     YFIQRKMLTPA"
     gene            complement(45304..46557)
                     /locus_tag="VFC74_01485"
     CDS             complement(45304..46557)
                     /locus_tag="VFC74_01485"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF044948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HZK24038.1"
                     /translation="MSFSKEPLASKIYISVVSIAAITFLFFHLPTRQMFSVSFFFFVI
                     LHIMLDQLDVPLPRGNSAVSVNFAVDLALIMLFGPTAAAWAGLSTVLNIRSLKSFRES
                     WYKMLFNASQMALATGLAGHAFILTGGINGRLNLSHDIVPILVSTFVFFFINTTLVML
                     AVTFSQKVSFMGVWLTSFKWSVPNYLSNCPLGVLIAVVFNSMAIPGVLLLIVPLMVAR
                     HTFIMYMDMRNQYMSTIKALTKAIDAKDHYTRGHSERVAKYTVWIGEELHLPEDYLEQ
                     IEYIALMHDIGKIGVPEHILNKPSRLSDEEYHLVMEHSAVGAEIISNIKFIGEHADIV
                     RHHHERVDGSGYPDGLTEKEISLGAKMIGVADAYDAMTSERIYRAPLTKAEAVAELQR
                     CSNSQFSEEIVKAFLKVLRRKGEIE"
     gene            complement(46799..47449)
                     /locus_tag="VFC74_01490"
     CDS             complement(46799..47449)
                     /locus_tag="VFC74_01490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24039.1"
                     /translation="MADTAKEVKEIRLIECESLIKRIRDVLSVNIVLGEQSEIVEVHV
                     LAEAGRNAKQIVRDIETLLQVEYGIDLDHKKVSIVQLNQGQKLAHEKRLNFTGISYSL
                     QGSQLEAVVELAADKRTCQGRSSGVNTRRNGIRLFAQATTEAIKQFLAPGATLIFEDV
                     AQFSLGSQTIIATTLTLLHDISEETLVGSALIRHDDKDAVVRATLAAINRRVPLEL"
     gene            complement(47733..48839)
                     /locus_tag="VFC74_01495"
     CDS             complement(47733..48839)
                     /locus_tag="VFC74_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24040.1"
                     /translation="MMLDVIIFEGNEAKSPVEEMLLTVRHAALRDNLNKLVAVPEVKR
                     IILATNHPEFKAYASLPKVIVEVNNIPPSQFQFGRQLINLIERYRVRTVLCMGGAAVP
                     LISVAELRWLCQQVLAKEQCFVTNNVQSADIIAFNPANILQHFPAAATDNALALLLRY
                     DAKFTQVLLPITLGSQFDIDTPTDLLVLAASPFGGENLRQTLKQLSLPTEKLQQVKKV
                     LEGQYADVAMIGRIGAPAMERLNKNLKVRLRVFSEERGMKALGRLERNEVVSLLGFWL
                     EKIGPERFFTYLAQTVSAALIDDRVLFAHHGKEPADADRFYSDLGLFEKVQDPFVREF
                     TRAAQQCSIPVVLGGHSLVTGGVQVLVEELTRRD"
     gene            complement(48985..49518)
                     /gene="raiA"
                     /locus_tag="VFC74_01500"
     CDS             complement(48985..49518)
                     /gene="raiA"
                     /locus_tag="VFC74_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HZK24041.1"
                     /translation="MKLIVRGKNIEVTEALKDYAEKKVSKIDKYFAYDTEAQVTLSVE
                     RGIHKVEVTVLINGMILRAEEKTNDMYASIDQVVDKLERQIAKYKTRINRKSRQTGSL
                     KAMNELPAIEEEKDLFVVKNKRFVVKPMPVEEAILQMNLLGHDFFVFVNAENNHEINV
                     VYRRHDGKYGLIEPALK"
BASE COUNT        13737 a        12601 c        10150 g        13224 t
ORIGIN      
        1 gttaaaagat gcacaccccc agaggtctaa tcgtttaaaa taagcacttg gcagccacaa
       61 gttcgtgtca agtatcctcc gaaaacggct actctaaaac accaactatt gctacaaggc
      121 aaaaagcaca aagcccgaaa gcggcgtaaa tcaagcactt tcgggcaaac aaaaaaccac
      181 ctgctgacaa aattcctttt atcaatagat ggtatttgat ttggtggagg cgaggggagt
      241 cgaacccctg tccgaaagca gaccagcaag agtttcttcg agcgtagtcc caactttagt
      301 tttacacaaa aaggctcctt gagacgggat cctgatgtgc tagcactgtt gttattccct
      361 tcctctttgg cagtacggac cgtgaggttg gtagcctgat tagtcggcgc ccggatttac
      421 ccctacaggc tcgcggtaag cggacgagct actttaatta agcagctaaa gcgtaattat
      481 cgttggcaat taagcctttt ccacgttgtt gaccgggtca tgggcccgga ctcgctactc
      541 tcacccatcc aacccccgtc gaatccaaaa cgcccccaag ttgattattg attactttcc
      601 ttaaaggctc tttctacttc acgtttggct tccttttctt tgagggaagc acgcttatcg
      661 tagagttttt taccgcgggc taaggcgatc tcaattttaa tatatccttt attagaatag
      721 actttagtgg gtattaatgt gtatcctttt tcccgggtga gaccgattaa gcggttaatc
      781 tcccggcggt gcatgaggag tttacgttta cgcagtggtt catggttaaa gcggttaccc
      841 tgctcatagg ggctgatgtg catattatgc acccaaacct cattgctgtc aatgcgggca
      901 taactatctt tgaggttaat gcggccggca cggatagatt ttacttcagt tcctgtcagc
      961 accatacctg cttcataagt ctcctcaaga tgatagtcat gaagagcttt tctgtttttg
     1021 gcaaaaacct tatctcccat aaaccaaccc tcattcaatg gcatcgtata atgccgccgt
     1081 ctaaagaacc ctatttgtct agacaaatag ggcgccactt ctccttatta caccaacaac
     1141 agtattataa catgtgtcgg atttttcgtc aacatcagaa caccggcctt gcggccggtg
     1201 ctttgataga ggggttttta gaaatcgtgt atctcgtctt tttcaccatg acaggttaca
     1261 cagttatctt taccttgaat atctttgccg tgacagctta cgcaatcatc agtaccttgt
     1321 gttttgtgga cagctacaaa ttgattgcta aaatgagcat tcagcatttc aactgtaaac
     1381 ttagctacgt cagcactgac accgccacaa cgttcaattt tatggtcgga agtaacttcg
     1441 agatcatttt cacttaagaa ggtagttact gaaacgtgac atagagtgga gttagctaca
     1501 gttggtttaa attctaaatc cgaaggtttg taataaggga aggttgcatt ttgataccaa
     1561 gccattagtt catttactat ggggccctgc tcctctagcg gaacaaaaag tccaatgaag
     1621 ccgatagcac tgattaaggt gccgcaagtg gttccccagc tcatgattcc gcccttggca
     1681 ttattataca tttgtggcgg gatgtaacta tagggagcac cgtgttcttc tgccattaag
     1741 ccgaaaagac cttcggcgac agcttggcct cagccagcac ctgacttgta tgagttgtag
     1801 gcaatttctt cggctttggc tgaatccaat tgatggtacg ctaggggcca ggtaacatca
     1861 acaggctggc catttggctc acctgttgtt tcttctgtag gcttactaca gccggttaag
     1921 aaaccaccca acccagcaga taaagctgtt acagctaatg ttgctccagc acctttaata
     1981 aatttacgtc tggttagttc tttttctttt actgccattt ttaatacctc cgcctttatt
     2041 tactagcagc ttaaagctgt tctagtctta ttctaaaaac tttagtttgt taattttagg
     2101 cctcactgct gccatcttta attgagtcac tgatttcctt tgtagcattt ttaaactcgc
     2161 ggataccttt ccccagtgat ttacccactt ctggtagttt gcttggtcca aaaattaaga
     2221 gcaccgctac taaaataagt agtaattcac ccaagccgat ttttgacatg ctactcccct
     2281 cctagtcaaa agcttgtgaa tctattacct tactttgtag ttcaaccttg atttgggcat
     2341 tccttctcga caaaacttgg acaaaatgga cgtaatttcg acaaaaagaa agaagccttg
     2401 taatataagg cttcttaggc aacttgcaga tttttgccat aatgaatgca aaacgctata
     2461 gacataagct atagcagtaa gttaggttat tagatatcgt aatagtgctt tgctgccgcc
     2521 ataggcaccg ataaaaacag ctaaagcaaa aacccagcca tgcaaagaca gtgaagcaat
     2581 accggtgagc aaagcaccaa tattacaacc taaggccagt cgggaaccat accccatcat
     2641 tatgccgccc actagtgctg agtaatagta tttattagta cggggcttgc gcagacgaaa
     2701 ttcatggtga cgtacgctag caacaaatga gccaagcacc atcgctagtg ccaaataaat
     2761 tatcggatgc tttagcagaa tagcggtagt attttcccta aacagctgat gttgaaaata
     2821 ctgccagctg ctgacgggaa gccccagcca agcagctaaa cctcccgcta aatgtgttag
     2881 accactacta ataccccatg atctatgcca gagagcaaat aacatggtat tggccaaagc
     2941 tagataaact gctcctttgc catatggcca gattttaccc tgctgtttaa aaattgacca
     3001 gagatccttt gctcccccat atttagcagc agtaaaatag agcaagctaa taattgacaa
     3061 ctgcagcacc tgggcggctt gccagccgac cacatcgggt aaaaagattg tgggtgtaga
     3121 agcaattatt gtgggcagcc accaattcaa atgccaggcg cctaaaagtg agcctagcaa
     3181 caaaccaaac aaggtcccta tctgcatcaa gtttccttca ccaatacgcg tgaggcagcc
     3241 agataagcag ctgccggcga tcaccatgcc aaaaccgaaa aacagtccac ccactagggt
     3301 gtgaacgcca accggataga cgcgtcctgc tgtacctaaa ctaccagccg taaaaaagtg
     3361 taccaacgca aagctaagcg tgttaataaa cagtgccact aaaacagccc gtgtttgtgc
     3421 cgtgttgcga attaaaaaga tatcacgaaa gcaagcagca aagcaaaaac gagatcgttg
     3481 aataatatag ccaaagatca gtccaaaaat aatgggatag agcatttgca ttctttggtt
     3541 aaacactcct tttatggcag cacatattta tttcttaaca taaagaaaag gacaagggaa
     3601 agacaataaa tgaaaataag aagggaattt taagttaaac gtgaaatata agagtaggaa
     3661 agatcaggtt tttccttagc acttgtaagg aagtgccagg tataaaatag gcaatttctc
     3721 cgcaatcaac tttttgggag ctgatctgat ggagcttgat aatttaaagg ccttttgcat
     3781 cgcagtagaa tccggtagta tctccaaggc agctaaaaag ttatatatct cacaaccttc
     3841 gttaagtgtc aaaattcaag aactagaacg ttattacgaa gctatcttgc tgaaccgcac
     3901 aaataagggc attcatccca cggatgctgg attaattgtc tatgagcatg cacaaaaaat
     3961 gctttccctt agtgatagta ttgagcgcag tttggagcag aaccaacagg acgtacaaca
     4021 attagttgtc ggcacctcaa gtacaattgg taactttgct ttgccttgta cggtctataa
     4081 ttttaatgag aaatatcctc attataaggt ctctctaatt attagcaaca cggaaaaagt
     4141 gctggagaat ttagcaacac ataaagttga cctaggtctg atcgagggac cgcttactaa
     4201 agaattaaaa cacactttaa cgcaagaggg tattaaaaca aaacatgtga tcagcaatcc
     4261 tttgattctt gtggtgccaa acgacgaaaa ctggcagaac atcaacagta ttcatcgtga
     4321 ccagatttca caactgccct taattatgcg tgaagaaggc tctggcattc attctacact
     4381 caagctgggt ttagctaaat taaatctagc attcgatgac ttaaatgtag tgatgcagtt
     4441 aaattctaca aatgctatca tttctgctgt agcctcaggc aagggggtca gctttttacc
     4501 acaagttgct ttgcgcaaag aactgcatta taaaatctgc aaggaagtca aagtgcagga
     4561 tgttgttttt cgccacagtt ttacaacact ctactatcct aacgacaatc agcacagttt
     4621 acagactgcc tttttaggtt ttttacattc tagtgaacgc ggcttttgct aatctttttt
     4681 aagaatggtg atctcccaga taccctcgcc cacttgcgtt aagctgcaag ggtatttgta
     4741 ctttgattca aaatgaccga caacactagc tgaagagcag gtatggtcag tcgtaataat
     4801 taccctgtca cccggtttaa tttgggtcaa ttttacttct gcccgaatga tgggaatggg
     4861 acagaattcc ccgattgcat caatgttgtg atccatctcg ttcaacctac cttaaacttt
     4921 tctattttac tgttcttttt taattctcgg ctgggctctg taatccctgc tccagagggt
     4981 gagctcaatg tggaagatat ttaaccaaaa aatagccaag aaccagcttc tgtactggtt
     5041 cttggctatt ttttctacta ttttactgaa aatatgccta gatagtttac ctggtgggga
     5101 cttagcaagc gtgtcaaaga accgtcccct gtcatgcggg ggctaaggtg aggcagcact
     5161 atatgatgtt gcagcgcaaa accgtcccct gtcatgcggg ggctaaggca aagtctagtt
     5221 ggcggtcttc tttgctggca cgtttaagga tgacacgaac aggttcacca aggcggaagg
     5281 tgttgcccgt gcgttcaccg attaacgcta atttctcttc atggtagtgg taatagtcat
     5341 catccatact ggaaacgtgg attaaacctt ctaccaggtt atctaattcg gcaaagagtc
     5401 caaatgaagt aacgccggaa atcacagcgt caaattcctg acctactttg tcagccataa
     5461 attcaatcat tttgacttca acgctttccc gctctgcctc catggccact ctttcccgca
     5521 gcgaggaacg ttcagcagcc acagtattac tcttgtaaat actcgctaaa cgcttttggg
     5581 gcggtacttt agtcaggtat tcgcgcatta ggcgatgaat caccaggtcg ggatagcggc
     5641 gaatgggaga ggtaaagtgc gaataatatt ctgcagctaa accatagtga gccaggcgat
     5701 ccggtgcata tttagcctgt ttcatggcgc gcagcaggag agtattaatg acccgctcct
     5761 ctggtttacc agctacctct ttcagtatct cctgtaaaac tcggggatgg attttctccg
     5821 gattaccttt tatggaaata cccaggttat gagcaaaatc acggaaatgc agcatttttg
     5881 cttcgtcggg gttttcatga attctgtaga caaaaggtag ttccaggcga gcaaaatgtt
     5941 cagccaccgt ttcattacag atgagcataa actcctcgat gatagtttca gcgatggttc
     6001 gctctcgttt gacgatatct acgggtttgc catcgtcatc aagcagtact ttgacctcgg
     6061 gtaaagcaaa gtcaatggca ccgcgggcaa tgcgtttgtt gcgcagcacc agtgccagct
     6121 ctcccatttc ctcaaacatg ggcaccaatt ttgtataacg ctgccgctgc tcgtcgtcct
     6181 gctcttccag aatggctcgc actgcatcat aggtcatacg ttcaaccgta cggatcacac
     6241 taggggtaaa ctcatgtgtg acgactttac cttgagcgtc cacttccatc aagacactca
     6301 tggccagacg atcaacctgg gcattaagac tgcagagatc attggaaagt tcgggcggca
     6361 gcatggggat cacgcgatcc accagataga cactggtacc gcgagcgaga gcttctcggt
     6421 ccagcaggct gccttcctta acatagtaac ttacgtcggc aatatgtaca cctaaaagcc
     6481 agttgccatt gggttgacgc tgcaaggaaa ctgcatcatc aaggtcttta gcatcagccc
     6541 catcaatcgt caccaattgc tgcgcacgca gatcacgacg gcctgctatt tcttcttcgc
     6601 ttacctcgcc gctgaagctt tgcagttctt ttttaatttc ccgagagaaa tcctctggca
     6661 gttcatactt tttaatgatt gttagtgtat cgacaccttt atcccctgga aagcctagaa
     6721 tatccaccac ttttccttcg gggttgcggc gtttttccgg ccagcgggtt atttctatct
     6781 gtattttcat gccattcttt aatcctttag cctcagcttt ggggagaaag atatcctgag
     6841 agatgcgttg gtcatcaggc acgacaaaac caaattgccg tcctgcctca tagcggccaa
     6901 caattaaact agaacgacgt ttcaaaatgc ggataatttc gccctcttgc ttccgaccgc
     6961 tgccaccttt acctaatcgt gccaccacac gatcaccatg catggcgcca ttcatttcgc
     7021 tggcaggcac aaaaacatcg gcatgtgtag tatcatcggg gatgatgaag ccaaagccgg
     7081 cttgattgcc ttgcaatctg cctactatca aattcatttt ttccggcaca ccatagcgcc
     7141 cataacgggt tttaattact tgaccttcct gttccatctc cactaacagt gtgttaaaat
     7201 gtttgatatc ctttttttca tttatgccta attcctgtgt tagttcttcg gtttttagtg
     7261 gcttgtaggc tacttcgcgc atataagcta aaattttatc ttgaaaactc atacctagcc
     7321 tcttttctgc tggataattg acaagcacaa acttgccttc tataaacgta gtataaacaa
     7381 aagatggttt ttaaatgagt ctgcaaaggg aaatttgcag atgatctaga ttaggcgctt
     7441 acagtataac atacaccttg gtaaataaaa gagcggcagc tgccgctctt ttgtgattaa
     7501 aagattaatt cgattagagg tacaatgcgt aggaaaagcg gggcgaaaac aagtgctacg
     7561 atactcatca gtttgatgag aatgttgagc gagggaccgg aagtatcttt aaaggggtca
     7621 cctactgtgt caccgacgac agcagcttta tgcggctcgg aacccttacc gccatgcgct
     7681 ccgccttcaa tatatttctt agcattatcc caggcaccac cggcattaga cataaagatc
     7741 gccatcaaaa caccggtaac taaggaaccg gctaagaggc cgccaagggc ttctttgcca
     7801 agtacaacac ctaccagcag gggcacaact accgccatta gaccagggag aaccatctgg
     7861 cgcagtgcag ctccggtaga gatatctaca catcgtgcgg aatcaggttt tgctgtccct
     7921 tccattaagc cagggatttc ccggaattgc cggcgtactt cttcaatcat ctcaaaggct
     7981 gcacttccca cggcttccat agttaaagcg gagaaataaa agggcagcat gccaccaatt
     8041 agcagtccga caataacata ggcattggtg atatcaatgc tttcaatccc tgccgcttgc
     8101 gcaaaggcag aaaagagagc taaagcggtc aatgccgccg agccaatggc aaaaccttta
     8161 ccaatcgcgg cagtagtgtt tccaatagaa tcaagttcat cagtgatctt acgtacagat
     8221 tcgtccagac cagccatttc tgcaatgccg ccggcgttgt cagcaatggg gccgtaggca
     8281 tctacggcga tggtcatgcc ggctgtggcc aacataccaa cggctgagat agcaattccg
     8341 tacagacctg ctgattggtt tgccaccaga atggccaggc aaattaccag caagggcagc
     8401 atggtagagc gcatacccac ggctaggcca gagataatgt ttgtggccgg gccggtttcc
     8461 gagctgtcag caatgtcttg cacaggacga taatgatcgg aagtgtaata ttcagtaatc
     8521 cggccaataa tgataccagc aactaaacca gcaactacag cccagaacgg gccgatgcca
     8581 ccaaactcaa agctatagta acggatcaca aaaaaggagg tgataactac taaaataccg
     8641 ctcaccagag ttccacgctc caaagaagat gatagtttta ctccttcttt ggctttcaca
     8701 aaaaatgtgc caataatagc agcaaagacg ccggaagcag caacaatcat tggtactagt
     8761 gcaccttcaa taccaaatgt agcgatacca atcgtcatag ctgcaataat ggaaccgaca
     8821 taagattcaa agaggtcagc acccatgccg gcaacatcac cgacgttgtc gccgacgtta
     8881 tcggcaataa ctgcagggtt gcggggatca tcttcgggaa taccggcttc tactttacct
     8941 accaagtctg cgcccacgtc agctgcttta gtataaatac caccgcctac acgggcaaaa
     9001 agggcaatgg aactagcacc taaagcatag ccattaacaa tttccggatc cttataaatt
     9061 aagtacagca ggcctaaacc aagtaagccc aagccaacaa cagacagtcc catcaccgca
     9121 ccgccggaaa aagcaatggt gagggctgga ttggtaccat gctgtgcggc attcgtggta
     9181 cgtacattag ccttggtagc aatattcata ccaatataac cagctattgc tgaggctaga
     9241 gagccacaga ggaagcaaat cgccgtttgt agatcaatgg caaaggtaat gacagcaaat
     9301 aaaatgataa caaaaatagc aagtgtgcgg tattctctgg ttagaaaagc catcgcgcct
     9361 ttatgaattt catcagcaat ttcctgcatg cgatcatttc cgggatccgc agagctaact
     9421 ttatttgcca aataaaaggc aaacaacagt ccaaccagcc ctgctgcggg aattaagtac
     9481 aagatgttgt ccatactttc tactccttct ttttcagttc tttcgtgctg caccgcagac
     9541 aagttttttt tcggcttgcc cagcgacgct aaatttcagc gccaaacgct gacagtctgc
     9601 ctagctcaag gatgttaaaa ttatggctag acccataaat agcacagcaa aaataattgt
     9661 cagtttggcc aagaaatcat cgagtccctt tttcttacca aataaagttt gtgccccacc
     9721 ggtaatactg ccggataagc cagctgactt tgatgactgc agtaaaacag aggcaatcat
     9781 ccccagccct gcaattaaga agatcaggtt caagacaaac tccatcggta aacacctcct
     9841 ttttaacgcc taaaaccttt agctattcta ccatagtcac tgttaaaaaa caataaactt
     9901 attatttacc aactaggcat tcttaaacgg attaccggcg taaagcgcac tatcagccac
     9961 ttgttcctca atacggagta attggttgta cttagccacg cgatcaactc tagaaggagc
    10021 ccctgtctta atttgtccgg cagcagtggc cacggccaga tcagcaatgg taacatcatc
    10081 tgtttcgcca gagcgatgag agataacagc attataaccg tgctgctgcg ccagctggat
    10141 acaatctaaa gtttccgtta ctgtaccgat ctgatttaat ttaattagga tagcattgcc
    10201 tgtagcacgg tcaataccca tttgcaaacg ctcaatattc gtaacaaata aatcatcacc
    10261 aactagctgc accttctggc ctagtctgtc agttagcagc tgccaacctt cccaatcatc
    10321 ttctgctaaa ccatcttcaa tggataccac cggataagta tcaactagtc tttgataata
    10381 gtctaccatt tcggcggctg tcttttgtaa tccttctccg gctaaatgat agaaaccatc
    10441 tttatacatt tctgtagcag ccacgtctaa agctaggacc acatctacac caggttgata
    10501 gccagcggcc gtaatggctt tgacaatttc ctcgagcgca gcttcattag aagatagatt
    10561 gggagcaaaa ccgccttcat cacctacggc ggtattgaga ccgtgctgct gtaatacttt
    10621 cctcaaatga tgaaaaactt ccgttcccat gcgtaatcct tctttaaaag tagcggcacc
    10681 gactggcatc accataaatt cctgaatatc tacattgttg tcagcatgct gaccaccatt
    10741 aagaatgttc atcatgggca caggcagcag tttagcatta gctccgccaa gataccgata
    10801 taagggtagt cccaaagcat tagctgctgc tttagctgtc gccatagaaa cacctaaaat
    10861 ggcgttagca cctagtttac ccttattgag tgtgccatcc aaagcaatta aagtttgatc
    10921 aagctctgct tgcttcaggg catccatgcc cagcaccgcg ggtgcaataa tgtcattaac
    10981 atggcgaact gcctgctgca cacctttacc caggtaacgt gccgggtcgc cgtcacgtag
    11041 ttctaccgct tcaaaagctc ccgtagaggc acctgaggga actgccgcac gaccaaaagc
    11101 gccactcgct aaccaaacat ctacttctac agtagggttg ccccgggaat ctaaaatttc
    11161 gcgggctaaa atctccacaa tttgcgtcat aaacttcaac ctcctaaact cattaattat
    11221 cagcaggcag caataatgat ctgcctgtca tcactgccgg tttgggtaag gccaacagtt
    11281 ctaaaattgt aggcgccaca tcagctaaaa taccctggga gtgtaagcgc cgaggtgttg
    11341 tcgagacata aataaagggt acagggtttg ctgtatgggc agtaaaggga tgctccgtct
    11401 gtcccgcggt catctgttcg gcattaccat gatcagccgt gatcagagcg gcaccaccag
    11461 ccgctaacac tgcttccaca acctcgcgta aatagtcatc cactttttcc accgcacaaa
    11521 cagtagcctc cagcacacct gtatgtccta ccatgtctga attagcataa ttcaggacaa
    11581 tcagatcatg tactccccgc tttatttcct ctatcacgcg tgctgtgacc tgcagggcgc
    11641 tcatttcagg ttgtaagtta taggtaggca ctttgggcga aggaatgaga atccgttctt
    11701 caccagcaaa aggtgtttct tcaccaccac taaagaaaaa agtaacatga gcatactttt
    11761 ctgtttcagc aatacgtaat tgccgtaacc cggcagccgc taccacttcc cctaaagttt
    11821 gacgcggatg ctcgggggga aaggcaacgg gaaccttaat gtcttcagca tattgcgtca
    11881 tacaggtgaa aaggggaaat ggcggcgctt ggccacgatc aaagcagcta aaattttctt
    11941 cagtaaaggt acgtgtaatt tggcgcgccc gatctggccg aaagttaaaa aagattatac
    12001 tgtccagggc attaattgtg gacacaggat gttcaccctg caaaatcacc gtaggactaa
    12061 cgaattcatc tgtttcttcc cgatcgtagg cactttctaa agcatgcaga gcacaagcgg
    12121 ccactgggcc ctcaccatag accatggcac ggtaggcccg cttgacgcgc tcccaccgct
    12181 gatcgcgatc catcgtataa taacgcccac tgacggtagc cactgcaccc acaccaagtt
    12241 gggccatttt ttcttctaat gctgctaaat actggcgggc actggttgga ccaacatcac
    12301 gaccgtctaa aatagcatga ataaaaactt tttttacttg ctcacgttta gccatttcca
    12361 gcaaagcaaa taaatgctgg atatgactat ggacaccacc gtctgataaa agccccatta
    12421 aatgcagtgc cgagtcactc tctctgacct gctgcatcac cgtcaacagt ttttcattct
    12481 cacagaaact gccttcacta atggctttag agatacggct taaatcctga taaacaatcc
    12541 gtccggcacc gatatttaaa tgtcctactt cagaattacc catctgaccg gcaggcaaac
    12601 ctacggcttg gccacttgcc attaagctgg tggtagggta tgttgcccat agggaatcaa
    12661 aaaagggggt ttgtgcttgg cagatggcat taccctcgtc ctctgcacct aacccccaac
    12721 catctaaaat gattaaagct agcggtctag gtcggctcat cagacagact caccttgggc
    12781 gcgattaatc aggtcagcaa atactgccgc atccaaacct gctccaccga ccaaggcgcc
    12841 atcaatttct atttcctgca taaaatcaag aatattattg ggcttgacac tgccaccgta
    12901 ctgaatgcgc acatctcgtg ccactttgag gctaaattgt tctgtgatag tattacgaat
    12961 atgtaatgct acttcttttg catcgtcacc cgtagcagct aaaccgctgc caatcgccca
    13021 tactggctca taggctacga ctacccgtct tacttgtgat ggatccaggc cagctaaacc
    13081 tacactgact tgttctgcaa tcaccggcat tgtttgttct gcttgtcgct gctgcagagt
    13141 ttctccgaca catagaatag gtgttagttc atgggcaaaa gcagccttaa cttttcgggc
    13201 cacttgttga tcagtttcgc caaacaattg acgccgttct gaatgaccta aaataacata
    13261 atcaacagcc aaatctttca gcagcagtgg tgaaacttct cctgtaaagg cgcctgtctc
    13321 ttcccagtac atattttgtg cgcctaggcg taagccagac tgatgacagg cctcattaat
    13381 aaggcctaac aaaggaaaag gcgggcagat cactgtctcc acgttacctt tctctaagag
    13441 tcccgcaaac tgctccacat aatcaacagc ttcacggaca gttttgtgca tcttccagtt
    13501 ggcagctatg attggtacac gcatcttaaa cctcctctgt ctgctttaac accacaatac
    13561 ctggcagctc tttaccttcc caaaactcta aagcagcacc gccaccggtg gataagtgtg
    13621 agattttatc tgccacgccg gctttttcca ccgcggctac cacatcgccg ccgccaatca
    13681 gcgaaaaagc tgaggccccg gctacggcct gcgctatttc gatcgtgccc tgagcaaagg
    13741 tgtccacttc aaagacaccc aaagggccat tccaaattac tgtacccgct tgcttgatgc
    13801 gttcggcata taagcggcgc gtctctggcc caatatccag gaccatttta gctgcaggca
    13861 cagcttcagc cttgacagtt tgtgcacctg cggcatcatc gataccagca gcaatagtga
    13921 catcaacagg caaacagagc tctacctgca aagctttagc ttcacgcata atttggctgg
    13981 cattttccac ttggtcgtct tccactagtg atttccccat ctgacagcct tgtgctttca
    14041 ggaaagtatt ggccatgccg ccacccagca ggatggtctc agctaatttg agaaaagcct
    14101 taatcacacc aattttatcg gcagtcttgc tgccaccaac aattgccagc actggttttt
    14161 gtgcattatg taaggttttg gttaaagtct ctacttcctt ttgcatcaag aaccccgcgg
    14221 cagcaggcaa aaactcggcc accccgacat tagaagcatg agcccggtgt actgtgccaa
    14281 aggcatctga gacaaagaca tctgccagtt tggcatatgc ttgcgccagt tcgcgctcat
    14341 ttttttcttc accaggatga aaacgtacat tctctaataa cattacctga cctggctgta
    14401 attgttgtgc taaccgctct acttcaggac ctaccacatc atcagtttta actactttaa
    14461 ctttcagtaa ctcttccagc cgctttgcca ccgcagtgag gcgtaattct tctttcacct
    14521 gccccttagg acgtcccaga tgactggcta aaattactgc tgctccctgc tgcagcaggt
    14581 acgaaatagt aggcagggcc gccctgattt ttgtatcatc ggccactgca ccttgttccg
    14641 tccgcggcac attaaaatca acccgggtaa aaacacgttt tccggcccaa gctacatctt
    14701 taaccgtctt gattgtcaag gtaacacctc cgctaaaaac tctgtcggtt tattttgcga
    14761 tagggcatga aagaaatttg ccgcccgccg acaacgacga gcggcagcta ttagaattgt
    14821 ttgccaatgt aagccgctaa atcaattaca cgacaagcat aaccccactc attatcatac
    14881 caggaaacaa ctttcaccaa attatctttg accaccaaag tagacaaggc gtccacagta
    14941 ctggagtgag aatcgccttg atagtctact gaaaccagag gttcttcaga gtaacccaag
    15001 atcccagcca tggcaccttc ggcagcgcga cggaaggctt cgttaacttc agccgcagtt
    15061 acatttttat taagttcagc cgttaaatca accatggaaa cggtgggggt aggtatccgc
    15121 acggcgaaac catcaatttt accttgaata cctggcagga ccagagatac cgcacgtgct
    15181 gctcctgtgg tcgtgggaat catcgacagt ccggcagccc gagctcgacg taaatcacta
    15241 tgggcaacat cgagaatctt ttgatcatta gtatacgcat gaatggttgt cattaaccct
    15301 ctgataaagc caaattcttc atctaaaact ttgcacagcg gagctaaaca gttggtagtg
    15361 caggaagcat tggagataat atggtgttga gcggggtcat aggcgtcatg gttaacaccc
    15421 atgaccaccg ttaaatcctc ttctttggct ggtgccgtaa taatcacctt ttttacactg
    15481 ccgcccagat gcttgccggc ctctgcggca ctacggaaag ctccggttgc ttcaatgaca
    15541 atatccacat ccagggcatt ccagtccagt ttagcaggat ccctttctgc caaaattttc
    15601 acttctttac catccagcag taaggccccg tctgtcgctt ctacagtgaa gggcacacgg
    15661 ccatacaatg aatcgtactt aagtagatga gctaacgtct tggggtcgcg tgtgctgtta
    15721 aaagccacca actccagctc agggttctca tgtgctaaac gcacacaggc tttaccgatt
    15781 cgaccaaaac cattgacacc aactctaatt gtcatgtttt tctcttacct ccaattaaaa
    15841 tatcttaggt ttaaaagctc tagctttatt aaggtgctta aacgggaatc ctctggacac
    15901 ttccccacaa ataggaagaa agcatcacaa ggcagtaaat ctcctatccg agcacaaaaa
    15961 ataattctac ataaaaacca aaaatcctgc taaatgatgt agcatattta ctaaattgta
    16021 tagcctaata gacagaataa gagtaattaa cacgcaatta ggcgaacaaa taatagcgcc
    16081 gctggcgcta tttgttctga cagagaaatt atctcgttgg caatcagtaa aacatttata
    16141 ctctttttcg tttactggaa gcaggaacac ctaattcctc acgatactta gcgactgtgc
    16201 ggcgcgagac cacaatgccg cgtgcggcta acagttcacc taacttttta tcactatagg
    16261 gtttatattg gtcttcagca gcaaataatt ctttcatatg acttttcaca gatgctgaag
    16321 aaatagcggt accgtgataa tcttctaccc cggaagcaaa gaaaaactta aaagcataca
    16381 atccacgtgg cgtctgcaga tacttattgg ccgtagtacg gctcacggtg gattcatgta
    16441 caccaatttc ctcagcaatc tggcgtaaag tcagaggctt taagtattta atgccttcat
    16501 ctaagaaagc ttgctgtatt ttaaccaagc attccgtcac acgatagagc gtcagacgac
    16561 gctgctccag actacggacc aaccagaggg cggcatcaaa gcgacttttc agaaagtcac
    16621 tggccacctg gttctcatta ccgagcaatg aacgatagta aggattaatt gttaaacgcg
    16681 gcatgttttg ttcatttaca atgatgacat acttatcctg caccttttcc accgacacat
    16741 caggtaccac atagcgcaca tctgcagccc cgccaatcaa gcgccctggt ttgggatcta
    16801 aagtgcggat aaaatcaatg gcagcctgca ctgccgctgg ctcaacctgc aaagcggcag
    16861 caattttttt ttgcttatta tctgccacat cctctaggtg ctggctgaca attgcttctg
    16921 ctagcggatg agcatccggc agctcctgca gctgcagcag taaacactct tgtaaatctc
    16981 tggcccccac acccacggga tcaaaagttt gaataacttt taaaattgct gtcagttcac
    17041 tgcatgccag accgcttaag gctgctaatt cctccatctt gccattaaca taaccattag
    17101 agtcgatatt gccgattaaa aactcgccaa tcctcttttc cgtagtggtt aaactgcaga
    17161 aaccaagctg cgaagaaaga tgttcctgca gcgatggttc ggcacttaag gcatagtcaa
    17221 cagccgcagc ggcatcattg gcgcgcgact gcactgctgg caaaggctcg tatccttgat
    17281 cccgtaaata ttcatcccaa tcaatagaat cattttctaa tttactgtcg acctgcactt
    17341 cttccgcttt tttatcttct tcctcatcga gatcaagcac agggttattt aaatattcat
    17401 tttgcaagaa agcttgtaat tccattactg gcaactgcag tagactaatg gcttgctgca
    17461 gttctggtgt catgattaat ttttgtgttt gttcaagctt cagattgtga gtaatgtgca
    17521 tctaacatct tcctttatcg ttattcggtt cggcaacacg ctaagcttgc aagaggaaac
    17581 tgaaaagcgc aagctttttt attacagtac atcttatgcc cgcaccgaat gatgatgaat
    17641 caaggaaact tgtcttaccg taaatatctc tactattatt gttcgccatg tatcaacaaa
    17701 atcctgtagc tggtgccact atctcgacac tagctatctt tcgctaactg tttactcagc
    17761 gtctccaact tacgcatccg gtggttaaca ccagatttac taagcggtgg ttctaagagg
    17821 tcacctagct cctgcagact agcctcaggg tgagccaaac gcaagcgagc tacttcattt
    17881 aaaggcgcgt ccaaggcttc caagccaacc ttttcatcaa ttaggcgaat catctccaat
    17941 tgacgtacag atgcatctac tgttttattc atattagcag tctcaaaatt aatcaagcgg
    18001 ttgacccggt tccgcaggtc cttaagaatg cgtacatttt caaagtttag taaagcatta
    18061 tgtgcaccaa tgacattaag aaactctaca atctgttcac tgtcctttaa ataaaccagc
    18121 cagccatttt tacggctaat ggttttagcc gtcacctgaa agctggccat taagcttact
    18181 aaatcatctg cttgctgctg ataagcagtg aaaatttcca aatgataaga gttctccgga
    18241 ttagtcacag agcctgaggc caaaaaggcg ccacgtagat aagcacgtcg gcagcatttt
    18301 tttttgcgta ccttttcact aatctcctgt tttaaatcac cgtctatgag cagacctaag
    18361 gcctgcagga tcttcagcac gtgctccttt tctttaatcc ggatcaaata aacattattt
    18421 ttacgcagtc taattttacg acgcactaat aattcagaat taaacttaaa tgtttgttta
    18481 agtatactaa aaatacgccg tgccacggca ggatttgagg taaccacatg caaagcaagg
    18541 cgtttttctg agatttgaat gttgccgcta tgtctgataa aagctgtcag ttcggctgcc
    18601 tgacaacatg ccccgcccag ttcccggcgc gccaattcat tcttcgcctg catcgcatat
    18661 gtcatgcttt caccccactt ttcttcagca agcctagcaa ataatcaagc taacccggat
    18721 cgctacaaga catcggtaaa aatagcacag gctaatttat cggggtcatg tcgcactagt
    18781 gctactggtg aaagcaaatc agcagctacc actcgcacac ccattgtttg tagccgctta
    18841 agatcaggct gcacaggaat agcaccttcg gcagcatatt tttgtagcag agcggcagga
    18901 atatgtaaat tattattaat caaaatggtg tcaaacaccc ctgtgccaac atgctctgtc
    18961 agaacctgaa aatgatcggc agcactaaat ttttcagttt cacctggctg ggtcattaca
    19021 ttacagacat atactttatg ggcagctgtg tcacgcagtg cctgcactac acccggcact
    19081 aaaagattgg ccagtacgct ggtgtaaagg ctgcctggtc ctactacaac cagctgcgcc
    19141 gctaaaatag acttacgggc agccggataa atatcggctg tcgcaggctc tatatataac
    19201 cgtcgaataa tacctccagc ttgcggtatc ctgctttgcc cacggatcgt gcgtccatca
    19261 gtaagttcag ccactaaatt cagtttagcc atactaaccg gcagcacctc gccagtgaca
    19321 gcgagcaccc tgctggctac gcgtatggcc ttttcaaagc caaactcttc cgtcagcgcc
    19381 gctaaaaaaa ggttgccgaa gttatggcca gctaatcctt cacctgacag gaaacggtgt
    19441 tgaaaaactt gttccagcag cggttcagta ttggccaacg ccaagaggca gttacggata
    19501 tcgccgggcg gtaagatgcc cagattttgc cgcaagcggc cggaactgcc gccatcatca
    19561 gctacactaa cgatagcagc cagttttgtt gtataattct tcaggccacg cagcagggtg
    19621 gaaaggcctg tacctccgcc caaagctgcc accatcaatt cttttccccg ttcataatga
    19681 ttagatgctg tagcttctat tttcactgtt gtaaactcct taacctaaac ttctagactt
    19741 tatcgatgtc gcgatactcc ttcaccaccg gataattttt accctgcagt atttttccta
    19801 gttcattaac aatggacacc gaacggtgtt taccgccggt acagccgatg gcgatcacca
    19861 attgcggttt cccttcttta atataacagg gtatgaggaa ctcaattaga tcgagcaatt
    19921 tgcggaagaa cttgtaggta atgctccatt tccaaacata atttttaacg agctcatggt
    19981 tacctgttaa ggggcgtaag ttctccacat aatgggggtt aggcagaaaa cgcacatcaa
    20041 atactaaatc tgcatctaac ggaatgccaa atttaaaacc aaaagctaca agagtaatca
    20101 agatattctc ctgcttacta tccggtgcat acaaagtagt tatctcttct tttaattcat
    20161 gcggtgtaag ataagaagta tcaattaatt tatctgcatg ggcgcgaatt tgagcaagca
    20221 gtttcttttc ttctttgatg ccttccgcaa tgctgcccat cgtcgctaaa ggatgacgac
    20281 gcctggtttc cttgaaccga cggattaaaa cctcatcgct tgcttctaaa aacagcactt
    20341 cataatctgt gcccatgcct tctagcattt gcagagcttc ctgcaaactg gcaaaaaagt
    20401 caccacctcg gatatcagaa actaaggcaa tgcggttaat tttaccttct gactgcactg
    20461 ctagttcggc aaatttcgga atcagcatcg gtggcaggtt atcaatacaa aagtaaccta
    20521 aatcctcaaa gcaatgcatg gcttgacttt ttcctgctcc agattgtcca gtaataatca
    20581 caaaacgtat ctgttccatg ttctcacccc ttctgccagt tatctattaa ttcatctttc
    20641 acaggggaag ttcctgcttt tctgcttgtg caggtgcgat attaacaagt cgctttaacg
    20701 gtagatttaa tctttcaatc agctggtaag cggctgtaaa atcaccaacc ctagcaggga
    20761 tgtgagcatc actattcacc acaaatttta cccctaaggg caaagctacc tgtacataac
    20821 cagccagttt gggagcatag gaactattaa tctccatcag tgtctcagtt tcagcgcaag
    20881 cctctgccag ggcgtgcgta tcaatatcta tctgcagacc aggatgggta atcgcgtaaa
    20941 ctggatagcg gcgcaccgca gcgcagatgg ctgctgtatt ctgctggcgc agacgtggca
    21001 cagtcaagcc taaccaccca gccaaaaaat tttgtgcacc cagccaaaaa gcagaccaac
    21061 tgcgtggccg tactagttta tgatagccta ccagtaagta atccagttca gagaggatac
    21121 tttctggcac atcaatggtg ccatctacat cgacaatatt tgcctctacg ccaagcaaga
    21181 tttccaattc aggatattca agacgcaaac tgttgatctc ctcccgcatg cgtcgaaaag
    21241 tgtctgcacc gcgcacgcca atgaaaaaat gacccggccc atgatcagca ataccaatag
    21301 tttttaaccc gcgtcgttgg gccgcctgga cattttcctt aatcgtgcct ttaccatgac
    21361 tatatattgt atgtgtatga aaatcagctg ttgcctgcat taaaatagca cctcctctcg
    21421 taaggtagtg tgcccgttaa cccggaattt ctaccccagg cagcgcgagt aatgattagg
    21481 ccgccaagct ggacgagtac ggcgcacaaa gtgtcgcccc gacataataa aacagtctcc
    21541 ctgctgaatt gcagggagac tgtcaatgaa atcaagctaa tggttatcct ttttgcgctg
    21601 ctaatcttgc tgcgttttcc cgtgcataca tggctgcatc ttcgtcggta atcttataac
    21661 cgaagaacat gatcatcgat gccagcaggt agaaaccagc gggaatgccg ccaaagatcc
    21721 acataaagcc attaacaaac tcaggagttg ctgtcatacc ggcagtatag ccaataatgg
    21781 acagaccgaa ggtcccaatg gcaccaccaa gagcgaaacc aactttaatg ggaatgttat
    21841 tcatggacat ggccacagta cggttgtctt taccagtggt gtacaaaccg tattcggcag
    21901 catcaataga atagttagga ccgaaagtca tatacaagta cattgaagct tgacgcagag
    21961 cggcaaagac aatgtagatc acaattgact cacgggcaaa taatgtaata cataaactac
    22021 aggcagccat cattattaag cctgtaacta aagccatctt cttaccaagc tttttaccga
    22081 tggaaggccc aaccatggca gcaaagaagc tgaccatcgt catggtcgtc atcgccacag
    22141 ccatcaagtt gaagttaccc agtacataga taaaataata agtttgaata cccatggcgc
    22201 ccatggcacc aacttgggtc aaagacatga ccagcacgta aattaaaagc tgactgttgg
    22261 taacaacgga gttaatcata tccttaacgg ttaccacagg accgccacgc ttggcatctt
    22321 cgggccgatc atatggtgca gaaactttta agatgatccc agcgccgata gcataaataa
    22381 gggcaaaggg gccagctaca gcaatataag cgttagaagc accaacaatt ggcgtgaaga
    22441 agttaagcaa aggaataatt gaagctgagg taatgattgc accagctgtt tgaaattgtg
    22501 cttgtctggc agaaagccgg tttctgtcta tcatggaagt tcccgccatg gaagccagaa
    22561 taccaaactg tgataaggcc acaaagttca tggagaagtg aagcataaaa taaccaacag
    22621 tcacaatcgc cagccttagt ggcatagcca aagaactagt attaataaac tgcaaagaaa
    22681 tacctactac tactacccac cgtaaaatca caaaccagga acggtattta ccccatttca
    22741 agtttgattt ttgtacaatg gcgcctgaag ctaaacaaac agcaaagtca agaatacggg
    22801 caaccaacaa taatgtggcc acaaccgcgg cactaatcat gagatggtct gtcataaata
    22861 gcacgccata gctcatgggg attgcaatac caagacctga aaacaaggca taggaaccat
    22921 aagtattaac ttcagcatta gttaatcttc caccactcat cactaaacct cctaattgtt
    22981 catagttctt actttaaaaa tccttaatac atgatgaaaa tataaattct gtaagttctg
    23041 acccatgaca accacaactt ttccaaatta aagctacccc atctacccct ccccagctaa
    23101 ttctgctgaa ttcaactgtc aaagcaacat aattgcccaa tgcgaactcc tattttctac
    23161 gccgacaatt ctagatatct tgtcaaatta tttagtgcaa atgctgtttt tttgtgctgt
    23221 gattagagag tttatgctgc cagtgactat ttcattattt taaatagtca gacaaattgt
    23281 atcatatttt tcaggcaaga acaagtaagt tttttgaaaa ataaggtaat tctagggtga
    23341 actttatttc actgcacaaa aaaaatctcc ctgctgtaag agcagggaga tttgcttgca
    23401 ttaagcttaa gctttttgtg ctgctagttt tgctgcgttt tcacgtgcat acatagctgc
    23461 atcttcgtct gtaatcttat aacctaacaa cataatcagc gatgccagca ggtagaagcc
    23521 agcaggaata ccaccaaaga tccacataaa gccagtaatg aactcaggcg ttgctgtcat
    23581 cccggcagta taaccaataa tggaaagacc aaaggttcca atggcaccac cgagagcaaa
    23641 accaacttta atgggaatgt tattcatgga catggccaca gtacggttgt ctttaccagt
    23701 ggcgtacaaa ccatattcag cagcatcaat ggcgtagttt ggcccgaagc acatgtacat
    23761 atacatggcg ccttgacgca ggcaggcaaa gatgatgtag gtgactactg aattacgggc
    23821 aaagatggta atacatacgc tgctggcagc cataatcatc aagcctgtaa ctaaagccat
    23881 ctttttacca agttttctac caatgctagg tccaactgtg gcagcaaaaa aactaaacac
    23941 agtcatggta gtcatggcca cagccattag gttgaagttg cccaacacat aaataaagta
    24001 atacgtttga atccccatgg cacccatggc accaacctgg gttaaagaca tcaccagcac
    24061 gtagattaaa agctgactgt tagtgacaac tgagttaatc atatctttaa ctgttacaac
    24121 aggaccgccg cgcttggcag cttcggggcg atcatacggt tctgcagcct tcgtgatgag
    24181 gccggcaccg atcactaaaa caaaggcgaa tggcgcagcc acaacaatat aagcattagc
    24241 agccccaaca attggtttga agaagttaag caagggaata attgaagctg aagtaataat
    24301 tgcgccagct gtttggaatt gggcttgtct ggaagagaga cgagttctgt cttccatgga
    24361 agttcccgcc atggaggcaa ggacaccaaa ctgtgacagg gcaatatagt tcatggaaaa
    24421 gtgaagcata aagtaaccaa taacgacaat agctagtctt actggcatgg gcagggaagt
    24481 agtattaata aattgcaaac agatacctgt aaatactacc cagcgtaaaa ttagtaacca
    24541 agaacggtat ttaccccatt ttaaatttga tttctgcaca actgcaccag cagtcaagct
    24601 gacaccaaag tcaagaatac gagcaactag caataatgtt gccacaatac tggtactaat
    24661 cattagatgg tctgtcataa agagcacgcc atagctcatg ggaatcgcaa tgcctatccc
    24721 tgatgctagg gcataagctc cgtacatatt aatttcttta gtagttaatt ttccaccact
    24781 cattactata cctcctagta tttataatag ttcttgcctt ggagagttat tgctaaatca
    24841 caactaatta aatataaatc ctgactaaac acccaattca ttcaacaact tgtcctctta
    24901 aaaaatttcc ctcgcctcgc ctatataacc cctccttacc agttatgctg tgtatagtta
    24961 aggtaaaatt cattacctac tgtgtctgat gctccgctcc tacttcctgc cttttcaaat
    25021 acttgctaac cttgactacc ttttgatgtt tctgaatttt atcatgaaaa aaagtaaagc
    25081 tgaaagtatc ttatgccact tatgaatatt tgataatttc agacaaattg tatcatatta
    25141 tttaagttaa ggcaataaga attttgtcaa tcaaagggga attaacaagg aaaataatag
    25201 gaaaggttta acaacctttc ctagataaga taagtttaga ttaagttatc tcaagtccaa
    25261 tcatgcaatt tagcttggtc tttaataata atgcgctgtt tatcgaggac aataattcct
    25321 tctttttgaa agcggctcaa gattctggcc acagtttccc gggaggtgcc aatcatactg
    25381 gccatctcct gacgtgttaa atccaaatct aaaaccacac catcttcctg cgggcggcct
    25441 tcaagacgta caaaacgcaa taataaccct gctaaacggg cataagtatc atgtaaagct
    25501 agatcacgca ccaatagctg tgcccgccgc aaacgcctgc tcataatgcg cagcagctta
    25561 atccctagct gtggctgtgc tgccaaaaga cgctccatat cggcattgcg cagcagccaa
    25621 cattgcgtat cttccacggc ctcagcggtt gcaggataag ggccacggtc gaacaagact
    25681 acttcggcaa aaatatcacc atcattataa atatgtaaaa tctgttcccg tccatctggt
    25741 gaggacttgc tgattttaac acggccgtgg ttaatcacaa ataaagattc tccacgttct
    25801 ccttctaaaa agagtactgc acctttagca tagttccgct caatcatcac actggaaacg
    25861 atctctagtt ctttttcagc taacccggca aagaagggta ctttctgtaa gtatttggcg
    25921 ctgcgctgca ctgtctccct cctcgcaagt cttaccttta ttaatagata atatgataat
    25981 caacagcttt gagattaaca cagcccgtca tcaacatgcc atgccgcaag tgctcggtca
    26041 tcgtctcaat aatcttattg acaccttcag ttccagcacc aaaagcgccc caaatagcgg
    26101 gacgaccaat gagcaccgca tttgctccta aagccagcat ctttaaagca tctatcccgt
    26161 tgcggatacc accatcagca aaaataataa tttcaccttt aacacgctca acaatctccg
    26221 gcagcacttc cgcaacaccc ggtgtgccgt ccagtactct gccgccatga ttagaaatca
    26281 caatggcggc ggcgccgatt tccacagcca tttccgcctc ttgagcggtc ataatccctt
    26341 tgagaataaa aggcagctta gttattttta ccagttcacg cagctggtaa aaacttttgg
    26401 ggcccaccgg ctggcctttc atggccatgg tcaccagccc agcaccgtcc agatcaatac
    26461 ctacagctaa cgcaccacta gcctctgcct ggcgaatacg ctcttttagc ggctccatgg
    26521 cccgtggctt aataatcgca ataccgttgt cagctttttc tatagcttct aagccagccc
    26581 gataaaattg cggatcagca ccatcacctg taaaagcgca cgaacctgct gctttagcac
    26641 cctcaactag atgggtgaca aattcttcct cacttaaagc cccgcccaga ttataggcaa
    26701 ccccagttaa aggcgccacc agcactggcg tggcaaaatt acggccaaat aacttaagtg
    26761 taagatcagg ttggtcaact tgatggatcg tacgcatatt taagtgatac atcgccaaag
    26821 actcgacatt gttctggaag gaggagccag ttaatagtcc cccgataccc ggcacttctc
    26881 ccgcacaagc acgtccatca caaacacggc acacacggca actgccacgt aagcgccccc
    26941 ttgcccgctc tctaatctcc tgtacattca tcctcgtgta gccccctcgc ttttcaccag
    27001 gtaattctat gtaaactgca atgcctgcag ctaggtactt ttcagcccca ttatcatatc
    27061 atatgcactg agcactttac cagacatcta accacaaaaa gagctttccc tgttgtacaa
    27121 ggtgcgggag aactgcgtcg cccaaacctg acttgttttt atgtaaaaaa aactgcgggt
    27181 tgagcccgca gcctggacat aactgttagt tatctgccaa tagggcactg agtacactgc
    27241 taatgattga caacaagagg gcgccaagaa taccagccca cagagactgc accacaaaac
    27301 ctttcacagt gtctgccact aaccacagca aggaaccatt aatgacaaat gtaaataagc
    27361 caaccgtcat aatattaacc ggtaaagtta tcagattgat caaggggcga ataatggtat
    27421 taacaaaacc ccagaccagg gcagctacca aagctgcggc aaagctttca atgtccacac
    27481 cattaatgac cagtgaggtc aaataaagcg ctagggcatt taatacccat cgccaaacaa
    27541 ggcttcgcat gcagatcacc tctatccttt attcttctgt gttgctagtt cgacgtattt
    27601 gtaaaaaaat cctcttactt ctacttaaat tatacttctt ttgccacttt gttggaaaaa
    27661 ctgttcactt acggtcagca tcctcctgaa aatatgcata gagagtggcc gcaacagctt
    27721 gattcatgcc ggcgaccgcc gccaattcct cgacggttgc ttcccgcagg gccttgatac
    27781 tgccaaaagc ctgcagcaaa gctttacgcc ttttttctcc gatgcccggc acctctaaca
    27841 acaaggagcc gcgggcagga cggttaagct gttttcgctg ttgagtgatg gcataacggt
    27901 gactttcgtc gcgcacacgc tgcagaagct gcagtgcagg tgaggtgcgg ggtaaacgca
    27961 gcggctgcgg ttggtcaggt aagtgaagca gctcttcttc ctcggagagg gcaataacag
    28021 gcagtaaagc atagtcctgt tctgttaaag cctgcagggc aacattcaac tgcccccgcc
    28081 cgccatcaat taaaattaaa tctggtacta acagaaaagg atcactggtt gttaccggca
    28141 ggtcaccggc agcccgggcg gccttcaggc gagcgaaacg cctgttgata acttctttca
    28201 tggaagcata atcatctggt ttatccacac tgcgaatacg aaaacgccga tattcagcag
    28261 tttgcgctaa cccgttttca aagaccacca tcgaggccac tgtcccttcc ccttggaaat
    28321 gagaaatatc ataaccctcg atgcgtaatg gcggtgactc taaatcaaca gcctcggcca
    28381 aggcctgtaa ggccacttgc ggatcccgcg ccttcacgcg cagcacttcc tgttctaaaa
    28441 agaggtgagc gttatctagt gccaagcgca ttaattcctt tttcgcaccg cgctgcggca
    28501 catgtaaacg tactttagtg ccgcgctttt gacttaacca ggctgccagc agctcaactt
    28561 cagacacctc ttcagaaagc aggatttctg ctggcagatc accggcatgt gtatagtagc
    28621 gatgtaaaaa agctgtgagc agctcggggg ctgcttcgcc tgtagggtta gtcaagaaga
    28681 aatgctcgcg acccgttagt ttaccgttgc gcacagcaaa taactgcgcg cagccactat
    28741 cttcgccagc agctagggcg ataatatcac ggtcgcctcc ggcagcgatg gcgatttttt
    28801 gtttctccga aatggctttc actgcccggt actggtcacg caatcgggcg gctttttcaa
    28861 aatccagctt ttctgccgct tctgccatgc gttgaactaa atcacggagc aatttatctt
    28921 ccttgccctc caaaaacagc agcacctcat ccaccagact gcggtactcc tctagcgaaa
    28981 cagtcccggc acagggcgct aaacagcggc caatatggcg gtttaaacaa ggccttttgg
    29041 cacgctcagc attaagcggc ccacggcagt ggcgcagtgg aaataagcgg tgcagcaaac
    29101 gaattgtttc cttaacggca ccagcattag ggtaaggacc aaaatagcga gcgccgtctt
    29161 tttccacacg gcgcgccaca tgaatgcggg gaaacttttc ctgtacggtc actcgtaaat
    29221 aaggatagtg cttatcatct tttaaatcaa tattatagcg cggccggtat tctttaatta
    29281 agttactttc taaaatcagt gcttctactt ccgtatccgt cactgtatag tctaaatcac
    29341 aaatcttttc tactaaagcc cgtactttcg gcgttaagtt gtgggcagcc tgaaaatagg
    29401 aacgtacacg ctgccgcaaa tcaattgctt taccaatata aataatctta ccgcgttcat
    29461 ctttcatcaa gtaaacgcca ggtcggcgtg gtaattgctt cagtttttct gctagcagtt
    29521 gcttgacctc actcatcatc aacctccgct aatgtgctgg taccaggtat tttttcaagt
    29581 accgcccggt atatgaggca ggcaccagaa taacttcctc tggtgtacca ctggctatta
    29641 cctggccgcc ggcgtcgccg ccttccggac ctaaatcaat caggtaatct gcccgcttaa
    29701 tcacgtctaa attatgctca ataactaaaa cactatcacc attctcgact aaacggtcta
    29761 gaatggtcag cagcttgtta atatcatcac tgtgcagacc ggtggttggt tcatcaagaa
    29821 tataaagagt cttaccattg ctgcggcggg aaagctcggt ggctaatttt accctttgtg
    29881 cttcaccacc agataaagta ggtgccggct gacctaaacg aatatagccc agccccacat
    29941 cataaagtgt ttgcatcttg cgcttaatcc ggggaatggg atcaaagaat tcaacggcct
    30001 ccgccaccgt catctccagc acatccgcaa tacttttccc tttataacga acctccagcg
    30061 tctcacggtt atagcgttta ccgtgacata cttcacaggg tacgtaaaca tcaggtaaaa
    30121 agtgcatttc aatgcgtaaa ataccatcac cgcggcaagc ctcacagcgc ccgcctttaa
    30181 cgttgaaact aaaacgacca ggcttgtagc cgcgcgccct gctttcgtta gtgcgtgaga
    30241 atagctcacg aatataatca aagaccccag tataagtagc ggcattggag cgcggggtac
    30301 gaccaatggg tgattgatca acatcgatga ctttgtctaa atattccaag cccagcaagt
    30361 catcgtgctc ggctggctta tgggtattac ggtgcagctc catggcgagg cggcggtaga
    30421 gaatttcatt aaccagcgta cttttaccag agccggaaac gccggtcaca caggtaaaga
    30481 cgccaagggg cacacgtaca tctagattct ttaaattatg agcacgagcc cctttgattt
    30541 ccagccattt gccattcagg ggacggcgct cagccggtac ccgaatggtt ttaacgccgg
    30601 ataaatactg tcctgtaatg gaggccggca cttctttgag gtctgcggca gtgcccgcag
    30661 cgactatgtt acccccccag accccggcac cagggccgac atcaataata tggtcggcac
    30721 gggaaattgt ttcttcatca tgctccacca cgatcactgt attacccaaa tcacgcaggc
    30781 gttccagagt ttcaattaag cgcgcattat cgcgttgatg caaaccaata cttggttcat
    30841 caagaatata aagcacaccc attaaactgg agccaatttg ggtagccagg cgaatgcgct
    30901 gcgcctcgcc accggaaaga ctccctgctg cacgatttag tgacaggtag ctcaaaccta
    30961 cattgagcaa gaagttcaga cgcgcctgca gctcttttac aatcaagcga gctatctgtt
    31021 cttctttggc acttaattgc agactggcca caaaatcttt cgctttatcc acagttagtg
    31081 atgtcacttg ggcaatattt tttcctccca cggtaacagc taaagtagca ggttttaggc
    31141 gcataccatg acattcggag cagagatgct cactaagaaa tgttgctaaa tgctcccggt
    31201 actgctctga agttgtttct ttataacgtc tcgtcagggt cgggatcaca ccattgtatt
    31261 taagctcata gtgctgaatg cggccgctgc tttcttcata atcaaaaggc agacgttcac
    31321 gaatccccca gagtactttt ttctgctgcc ctttgctcag cagggcaaaa ggtttatcca
    31381 cgtcaatctc atgatgctgg gccatggcta aaagcaactg atagtaatag gtgcccggtt
    31441 tccagggcac gacggctcct tcccgcagag aacgctgggg atcaggaatt actagctccg
    31501 gatcaaattc tgaagtagag cctaaaccgc tgcaggcggg acaagcaccg taggggttat
    31561 taaaagaaaa catcctcggt gttaattcct caaaggagaa gccgcattgc ggacaggcaa
    31621 aggcagagga gaataacaat tcctcttgct caatgacatc aatcagcacc aaaccattcc
    31681 cgtactttag ggcagtttcc aaggaatcgg ccagcctgtg ctccaagcct ttcttgataa
    31741 ttagccggtc aaccacaatt tcaatatggt gctttttatt cttgaccagc tcaatttctt
    31801 cttccagttc gcgcagctca ccatcaactc gcacccgcac aaaaccatct gctcgtacat
    31861 cctgaaaaag cttacggtgc tcacctttac ggccacgtac cagtggtgcc agaacctgaa
    31921 tcctggtctt gtccggcagc gccataatgc gatccaccat ctgctccact gtttgttggg
    31981 caatgggaag atcacagtgg gggcaagagg gatgaccaat gcgggcaaaa agcaagcgca
    32041 gataatcgta aatttctgtc accgtaccga ctgtagagcg cgggttattg gaagttgttt
    32101 tttggtcaat ggagatcgcc ggagataaac cttcgatgta atccacatca ggcttgtcca
    32161 tttgaccaag aaattgccgc gcataggcag agagagattc cacatagcgg cgctgacctt
    32221 cggcataaat cgtatcaaaa gccagcgatg atttgccaga gccagagacg cccgtaatca
    32281 ctactaattg attgcgcgga atatcgacat taatattttt taagttatgt tcgcgggcac
    32341 cgcgaatact aattgtgtcc tgtttcattt attctaaacc atcctctttt ccagccttac
    32401 gcagtttaaa aatcaaatcg cgtagttgtg cggctcgctc aaattctaaa tctttcgctg
    32461 cctgcttcat ttctttccct agtcgttgta tttctttctt ccgttctgcg gcactcatct
    32521 caccggcagc cctagctgtg gctgcatagc gcggcacttc ttctgctgtt ttgctggctt
    32581 caatcacacc acgcacttcc ttttgaatgc tgactggtgt gatgccatgc ttttcattat
    32641 actccgcctg gatagtacga cgacgctctg tttcatcaat ggccaggcgc atggaatcgg
    32701 taattttatc cgcatacatt ataacttttc cgtcaacatt acgggctgcc cgaccaatag
    32761 tttggattaa tgaacgatga gcacgcaaaa atccttcctt atcggcatcc aaaatagcca
    32821 ccagcgaaac ctctggtaaa tccagccctt cacgcaggag attgatgccc actaagacat
    32881 caaattcacc taaacgtaag tcgcggatta tttccatgcg ttccagagta tgaatctccg
    32941 agtgcaggta acgcaccttg agacccagct ctttataata ctcggtaagg tcttcagaca
    33001 tccgtttcgt cagcgtagtg atcagtacgc gctcgttttt ctccacccgt tgacgaattt
    33061 caccatataa atcatctatt tgccctttga ccggccggac aataactgcg ggatcaagca
    33121 gtccggtcgg gcgaataatt tgctccacca cctgctggct gtgttcttgc tcataggggc
    33181 caggtgtagc cgagacataa acggcctgac agactaactc ctcaaattcc ggaaacatca
    33241 gcggccggtt atctaaagct gacggcaggc gaaaaccata gtccaccagc acctgtttac
    33301 gagaacggtc accggcatac atgccgcgaa tctgggggat agtttggtgc gattcgtcaa
    33361 tcagcagtag aaagtcctgg gggaaataat ctatcagggt aaagggacgg gtcccggcgg
    33421 tacgcctgtc catgtgccgg gaatagtttt caatccctgt acagaagccc aattcattca
    33481 tcatttccaa atcatatttg gtccgctgct ccaagcgctg tgcttctaaa agtttacccg
    33541 tggcacggaa ctcctgcaaa cgctcttcca gctccaagcg aatatcaacc atcgcctctt
    33601 ccaaaacatc acgggaggtc acatagtggg aggcagggaa aatagtgata tgattgcgct
    33661 cggtcagcac ctcactagtg agggtatcaa tttcccgaat gcgttctatc acatcaccaa
    33721 agaattcgat gcgcaccgct tgctctgtgg cgctagcagg atagatttcc aagacatcgc
    33781 cgcgtacacg gaaacgactg cgttccagct gcaagtcatt acgctcatac tggttagcta
    33841 ttaactcccg caccacttgt tcacgactct tctccatccc aacgcgcaat gacaccatat
    33901 gggcactata ctcatccggt gaacctaaac cataaatact ggagacactg gctactatca
    33961 gtacatcacg acgctcaaaa agagcactgg tcgccgagtg acgcagcttc tcaatctctt
    34021 cattaatgga agagtccttt tcaatatagg tatctgtata cggaatatat gcttccggct
    34081 gatagtagtc ataataactg acaaaatact ctactgcatt gtcagggaaa aactctctaa
    34141 attcgctgca cagctgcgct gccagcgtct tattatgtgc aatcaccaag gtcggtcttt
    34201 gcacctcttc aactatatta gccatggtaa atgttttacc ggagccagtc acgcctaaca
    34261 aggtttgatg tttccagcca ctttctagac ctgctactag tttcctaata gcttgtggtt
    34321 gatccccttc tgggcggtaa ctactctgta actgaaactt gcccatttaa attctcctca
    34381 actagtttct ctgcactgct tcaagtttac ataacatatt atatcataac aagacaaact
    34441 ttttgcaccg tccatttagt ggcttttgtt tattgtaata ctattcctgc tctaatgcca
    34501 taaatattct cttacctggt aattttgagc tgcggctttt cctccccagg atctcgggga
    34561 cgaactgcag atgcccaaaa cttaaagaga cctgcaaatg gtaaaaacca gtacaaactt
    34621 gatgtactgg ttttatctgc atctaaaata ttactgcagc tctgggggct gactaaaaat
    34681 ccacggtggc agctgcccgt agttttctgc cgctgaacca tctggcaaag tggtgttctt
    34741 aagttgagca aagtcttgcg actgccgcag attttgatga aatttcaaat ccgctgtcag
    34801 ctgctgctgt actaagagca gggcattcat cgcctgctgc agaccttgta aggcttggcg
    34861 atcccgctcc tcctgccgcc gacacaattg cttgagcaaa aactcttttt cttgtaaatt
    34921 ttgctgcatt tctttcactg cacatccacc ccctctaaac ttctagtatg tgctattttc
    34981 acattttcat cagcagagca aggggcatac agtccagttt attgcattgc tttagtcaaa
    35041 atctccaagg ccttcttttt ttgcacatcc agctcttcca gccggggttc cagttgttca
    35101 tttaattttt cccgtgtcgc ttggttgacc tttccgttta cttccagacc atatttgctc
    35161 tggaaggaac gcacagcttt ttccgtctct ccatcaaatt tacttgtttc ttggaccgca
    35221 taatttaagg ctgctaacat cttctgcaag aaaagcacgt tggtgccttc atcactcagt
    35281 gctaaatccc ctatcatccc atactgcaga agataatagt catctgacag ctctactatt
    35341 acatccggca ccaagccatt gccatgaata tcgcgaccct tgggtgtttg gtatttagcc
    35401 actgtataac gtaggccagc ctggttagac aaactctcta aatgctgcac agtagctttc
    35461 ccatatgttt tggtacctac gagagtcccg gcagcagtat cttgcagggc accagcaata
    35521 atttctgagg cggaggcact agcaccgttg actaaggcta caatcgggta tgtttttttc
    35581 gtcccatagg attgataggt ttgcagcacc gtcccctccc ggtctaccat atgcgtaatc
    35641 ggtcccgctg gtaatatttc ctggccaacc agtacagctt gatcgagcag gccaccaggg
    35701 ttgttgcgta aatccagcag caggccgcgc attccttgcg tctccagtgc caacatcgcc
    35761 tcctgaaaat cacgacctgt atgctcgtca aaagtggtaa tctgaatgta gccgatttta
    35821 tccgacaata gttcagtaaa cacactgggt actacaatat tagcacgtgt aatgacaaac
    35881 tcaagcggct ctgtgagatt ctctcgttct acttttaagt taactgtcgt cccttcctgt
    35941 ccgcgcatta atttcacagc atctgtggtc gagataccta cggcatcttc accatcaata
    36001 gccacaatac gatcccctgg ttgtaagcca gcctgttcac ctggagtatt cttgatcggg
    36061 gacacgaccg taatataacc atcgacttca ctaatctcaa taccaatacc agaaaatgaa
    36121 ccgtctattt taatcatcat ttcttcatag ttggtagggt caagaaagtt ggtttgcgga
    36181 tcttcaagcg aattgaccat gccattgatt gcttcattga ttaattcttc ccgcgaaaca
    36241 tcttccaaga aacgttcgct taaaatatct aacacttcta ctagtggttg taattctact
    36301 tctatttctg ctaaatcccg agaattatct gctggcggct cacctgccgt ttcttgctga
    36361 tagtgatccc ttgtgccgaa atatgttaaa ctgtttgtca ccactaacag cagtaccagc
    36421 agcaaaacaa ttcttctttt caaaagctta acaccaactt tcaagcataa atttgagtta
    36481 tccctaacat tataaaccct actcggtaaa tctgccaagg gcagttctcc tcagcaacaa
    36541 aggctgctgc ttcatcctat tacaaatcct gcaaaatatt atcagcatct ctttttagtc
    36601 atcagctgca gcgtaggtaa agccgcctgc taaaaggcaa acgagagcat taactcgcca
    36661 aatatgaaca agcccggtca ctgaccgggc ttgttttaca ctgccaaatt aatgtacata
    36721 attgagtgga ttaacacgtt ctccgttaat ccgcacttca aaatgcacgt gcggccccgt
    36781 ggaataaccc gtagaaccaa cgatagctac agcttgtcct ttataaactg cttgtccatt
    36841 ggcaaccaaa aaatcatgag tatgaccata aactgtggca acacctttac catgatccac
    36901 aatcactaaa ttaccatagc cgctccccat gcgatatgta tgagctacac cggcatcggc
    36961 agcaagcagg tttacaggat taccgttata gttacccgca cctggccagc ggttacgggg
    37021 aattcctata tcaatcccgc cgtggaaaga accttgttta ccagagatgg gatcacgccg
    37081 atagccataa ccggaagtta tccaacctct gccgtaacct tgtaccggcc atcccagcgt
    37141 tcccgtacct tgattagcag aaccattttg ctgtgcctgc atttgcagaa tcaaattttc
    37201 aatcttttgg gcttctgctt ccagtttcct aatttcgtta tttgttttgt tgtaagctgc
    37261 ctgcaaatct gagactaaca atttctgcct ggcttgctta acttcctgct cttgtttttt
    37321 attaatgtta ttccgccgta gtgttaacag ctcctgacgg cgcttctcta atgattcttt
    37381 tagttcggca acccgatcgc gttctgccat tgcttcttct aaaagctgct tatcctgatc
    37441 tattaattgc cgcaaatcat tataacgagt taaaaagtcg gcaaaggatg tactgctaaa
    37501 gagaacttct aaatagctaa cctgcccgtg ctcgtgcaga gcccgcaggc gcacagccag
    37561 cacttcatct atctctgcca atttttcttc tgcttctgct aaatgagctt caacagccgt
    37621 gatttcctct tctgttctcc taattttcgt cccgatagca cgcagttcgg cctcaataac
    37681 ctctacttcg cgctgaatta actcaatttc ctgcttgact ttattttgtt cctgcttatt
    37741 ttgctgcagt tcttcctgac gctggtcaat attctcctgc acaccttcta attcatcttg
    37801 cttttcatca accgtattgg ccaaaacagg cagcatgctg ctcaataaca tcgctaccac
    37861 cagtagtaac agtacggcat actttcgttt aaacatcaca attcctcccc catcaaactt
    37921 tgagaaagcg ccgcatcgaa agcaggcttc ctaaaccacc tatggtgata ccgagcagca
    37981 gcaacagttt ggtaattgaa gctaaaatca tctgtggagg cagcagcggc agaaaaaata
    38041 aattcatttg tgtccaagcc accacctcgc ggtaaacgta aatcagaatt aaggctggca
    38101 gcagggcacc tactgtaccc agaacaatgc cttcaatcac aaaaggccaa cgtataaacc
    38161 attctgtggc gccaacccac ttcataatca ttatttcttt ggacctggca ttaaccgtga
    38221 gcttaatggt attggcaatt aagaacatcg ccgtaaaggc taacccgccc ataaatgcca
    38281 gaccgaccca gcgcacggca cctgtaaagc gaaagagctt gtctactaca tccgtgccgt
    38341 aatctacgcg ggcaataccg ggcagtgtct gcaaactttt cgccactgtg ggaatatctt
    38401 cgggaacttt cgtgcgaatt tcaaaagaat cacgcaaagg attacgaccc tcttcttcat
    38461 accccgccac aagttcacct aactggtttt gtaaacgcac caaggcttgg tcttttgtca
    38521 catagcgcac ttcttccaca ttggacagtt gaattacagt aacacgcaat tcctctacct
    38581 cgctgggggc aagttcctca tcaacataag caataatctc aacctggtct ttaatatctt
    38641 gcgtgatatg ttccacattg agattgagca gcataaaaga acctaagata agtaaggtca
    38701 ccgtcactac ggcaacagag gcgaaactca tccaaccatt gcgaaaaata ctgcggaagg
    38761 cttcctgaaa ataatactta atatttctaa tgagcatggt ggttatagac ccccttctgc
    38821 tcatcgtgca cgatttggcc gtcttccagc acaacaaccc gctgcttcat gcgatccaca
    38881 atctctttat cgtgagttgc cataattata gtcgtaccac gaatatttat agtttttaat
    38941 aaatccacaa taccccaaga agtttgtgga tcaagattgc ctgtaggttc atcagctatc
    39001 accaaagagg gctgattaat aatggcgcgg gcgaggcaga cacgctgctg ctcaccgcca
    39061 gataattcat gcggtttacg ctttgctttg cccgttaggc ccaccatctc taaagcctcc
    39121 ggcacgcgac gacgtacctc acgcggcgtt gcccctacca cttgcatggc gaaagccaca
    39181 ttatcataaa tagttcgttt atcaagcaaa cggaaatcct gaaaaaccac accaatacta
    39241 cgccggaaca ttgaaacttc gcggcgccgc aagcgtgtta tttctttact aaaaacccga
    39301 atactgccgg aagttggcaa aaactcacga ccaatcagac gaattagagt cgatttaccg
    39361 gcaccgctgg taccgacaat aaaaacaaat tctcctcttt cgacactaaa gttaatatcg
    39421 ttaagtgctc cgacactatt cggatagacc atattaacgt gctgcataac aatcatgatg
    39481 taaaggtccc tcctgtctgc acatctagat tataattaac aaaaccttac aaaattgttg
    39541 cgactattta acataaattc gacagacacc ctaaaaaacc tttaactacc tgcatttttt
    39601 ttcatggttt tttctccctc actctatcac ctgacaccgt ttacacctgt cctaccgaca
    39661 gagttttcaa aaacctcaca acatcaacat cggtcttccg gtgcagtgca gtaaagtaat
    39721 gctatcgccg gttttgcagc aacaagttat tgacaaaaaa aaacgccgct gccggctgct
    39781 gtttttttca ggtggggaag aatacagttc ggtccgacaa gcgacaggcc aagtgaatta
    39841 ttcaagcctg accccgcgtg ggactgaatc cttcgcctaa aacttcgtgg gcattaccta
    39901 ggatcacgaa ggctttagca tcgatttcat gcacaatcgt cttcaaacgg ggcacttctg
    39961 attgcgccac cacagttaac accatccggc gcttctggcc agtaaaggca ccttccccgg
    40021 ttaggattgt tgccccgcgg ttcatttcgc gtaagatgcg tgccaccatt gattccgtct
    40081 cgctagagat aattaaggcg gctttactgc tgctaagtcc ttcttgaaca aagtcaatta
    40141 cccggctggt gacaaataaa gaaatcaagg catacataga taattccaaa ttaaaaacaa
    40201 taccggctaa ggcgaccacc aggaaatcta cacctaaaag actttgtccc agggaaaacc
    40261 caaatatttt atttaaaact tgcgccgcca aatctgtacc gccagtggtg ccatgggccc
    40321 gaaaaaccag tgctacccca atgcccatca cgatgccgcc atacaaagaa gctaacaata
    40381 aatcctctgt caccaccggc aggaaggtca gcacttctat catcatcgag agcgccacgg
    40441 caccaaacaa gccgcgtgta atatagtgcc agccaaacag tttgaccgca attgcaaaaa
    40501 gcggaatatt aagcaagaaa atagtgatac ccacaggtaa gttaaagaga taataaagca
    40561 acacccctaa gccgctaatg ccgccagcag caatttgatt gggtgtcata aacatgttaa
    40621 aggcggcagc aatcactgct gtccccatca gcacgccaat atattctttg agtagctgcc
    40681 atttagccac gtgtgcaccc ccagtattta aacatcctta ccagcggttt gccgtaaata
    40741 tgattcgatc agctgatcta gttccccatc cataaccgca ttgacattgc caacgtccac
    40801 gccagtgcga tgatccttca ctaaagtata gggatgaaaa acataagaac gaatctggct
    40861 gccccagcca atttccttct gctcgccgcg cagattgcta atctttttta atctctcacg
    40921 ctcatagtaa tcagctaaac gcgcccgcag aatagtcagc gcccgttcac ggttcgaatg
    40981 ctgcgaacgt tcattttgac actgcacagt aaagcctgtc ggtaaatgcg taatccgcac
    41041 cgctgagtcg gtcgtattta catgctgacc accggctcca ctggcacggt aggtatcaat
    41101 ttttaagtct tctggtcgca gttcaatatc ctggtcagta tcttccacat ccggcaggac
    41161 atcaagcgag gcaaaagatg tatgtcgtct gctggctgca tcaaagggag agatgcggac
    41221 cagacggtgc acgccccgtt ctgcttttag gtaaccgtag gcattcttac ccgtcactaa
    41281 cagggtagca cttttaatcc cggcttcatc accgggtaaa aggtccagta cctctacctg
    41341 gtaacttttc cgctccgccc agcgcgtata catgcgcagc agcatggcag cccagtcttg
    41401 tgattctgtg ccccccgctc ctgggtggat agcaataatc gccgagttat ggtcatagcg
    41461 gccgcgaaaa attacagcta gctctaattt gcccaactct ttctgcagtt cagtaatgcc
    41521 agctgcggct tctgcccata attcatcatc gtcttcatcc tctgctagcg tgatcgtcag
    41581 ctgcaaatca tctagctgcc gctgtgctgc ttccaaaggc ccaatttcat cacgccgggc
    41641 atttagttcc tgcattatct tttgcgcttg ctctgaattt gaccaaaaat ctgactgcga
    41701 gacctgaaga tccagttttt ttatttgttc ttgttggccc accaagtcaa agggaaacac
    41761 tcatttcggt aagacgttcg ctcaactgtt gataacatgc tttgatttcc gcagacatct
    41821 ctacacctac tttccacaac attttttata tttcttacca ctgccgcagg gacaggggtc
    41881 attacgtcca acttttttag cactagccgg gccatgtacg gctgtaccct ggctcggcgg
    41941 agtttcattg gtaacggcaa cggcttgccg ctgtggcggt tccactaact gcacctggta
    42001 gagcagggca acagactcct cttgaatttc tgcaatcatc tggtcaaaca tatttttggc
    42061 ttcatatttg tattcgacca gggggtcacg ctgaccgtag gcgcgtaagc caatctcatg
    42121 gcgcagcata tccatagcat caatgtggat catccatttg gcatcaacag cgcgcaggat
    42181 aacaacacgt tccagctcgc gcatcttttc agcacccagc tttgcttcgc gcgcggtgta
    42241 agtagcaacg gctgcctcca gcaatagtgc aaacagttcg tcacggtcat tttcagctaa
    42301 agcagccgca gtccagcgtc gcggcggcaa aaagagtgct tccgcccaag ctaatagtcc
    42361 gcgcccatcc cattcttctg caaaaactcc cgccggtgcg tactggtcca tggcgtttgt
    42421 cagcagttcc ggcagccatt ctaagactgt ctcacgtaag ctttcaccct ctaaaacacg
    42481 ccggcgttgg ctgtaaatta cttcacgctg ttcattaata acattatcat attccaaaat
    42541 atgcttacgc gtttcaaagt tacggttttc taccttcttt tgcgctgatt ccagggcacg
    42601 gctgatcagg ggatggtcaa tcggtgtatc ctcatctagt cctagctttt ccatcacccc
    42661 gaaaatgttt tcaccgccga agaggcgcat taaatcatct tccaaagaaa tgtagaattg
    42721 gctggagccc gggtctccct gtcgtccggc ccgaccacgc agctggttat caatgcggcg
    42781 agattcatga cgttctgtcc ccaggatatg caagccgcca accgccagca cctgctcgcg
    42841 ctcctgtttg ctttgttctt cgtaagctgc cagcagctgc tgataacgtt ccttagctgc
    42901 agctttgtca ctttcttcaa ccgctgtttg ttgtacatat tgttctagct cacaaccaaa
    42961 ctgcgcacga aaatcatcgt tggccaatgc ttcgggatta ccacccagca ggatatcagt
    43021 accacggcca gccatattgg tggcaattgt tacagcacct aaacggcccg cttgcgcaat
    43081 aatttttgct tccttttcat ggtattttgc attaagcact tcgtgacgaa caccgcgttt
    43141 ctgcaggaga cggcttaacg cttccgagat ctcgatggaa atagtcccca ccagcagcgg
    43201 ctgccctgtt tggtgtctcg ctacaatctc ttctaccacc caccggaatt taccattaat
    43261 agttttgtaa ataacatcag gtaaatcatg gcggatcatc ggctgattgg taggggtcac
    43321 cacgacatcc atcccgtaaa tgctctggaa ttcggtttcc tctgtcgccg cagtacccgt
    43381 catccctgct aacttcttat acatgcggaa gtaattttgg aaggtaatag acgccaaggt
    43441 catgctttct ttagctacct tcacccgctc tttggcctca atcgcctggt gcaagccttc
    43501 actgtagcga cgaccataca ttaagcgccc cgtaaactca tccacgataa taatctgccc
    43561 gtctttgacc acataatcac ggtcacgttt cattaaataa tgggcgcgca aagcgttttc
    43621 aactttcttt ttcagaaagt ttttatcctc aatttcctgg ctgtctgaat cttcttgttc
    43681 tgcctccagc tcgtcaaaac taaggtattc ttcaccgtac agtcccttca cacccagtaa
    43741 ggcttctatt tgatactcgc cttcctcggt caaaagggct gatttagctt tctcatcaat
    43801 agtgtaatgc gactccgctt ttaaacggcg caccgcttgc tcaacgcgct cataatcctg
    43861 atgctgctgt tcatcagtct tcatttgtcc agagataatt aatggtgttc tcgcttcatc
    43921 aattaaaata ctatcgacct catcgacaat ggcatatgac atgggacgct ggaccaagtc
    43981 ttcctgatag cgcaccatat tgtcacgcag ataatcaaaa ccaaactcat tatttgtccc
    44041 gtaggtgata tcggcatggt aagcatcacg ccgctctaga ggcgataaac catggacaat
    44101 taaacctact gaaagcccta aaaattcata gagtggaccc atccattcct tatcccgtga
    44161 tgccagatag tcattaacgg ttacaatgtg tacgccatga ccagctaaag catttaaata
    44221 ggcaggtaaa gtcgcgacta aggtcttgcc ttcacctgtt ttcatttccg caatgcgccc
    44281 ttgatgcaag ataataccac ctaaaagctg cacatcaaaa tggctcatgt gcagcactcg
    44341 ccagcttgct tcgcgcacta ccgcaaaagc ttctggcagg agctcccaca aggcagcctg
    44401 ttcgctttga aacaactgct gctcggcttc agccagctca cgccgcgcaa agctatctgg
    44461 attttctggg agatgagact taatttcatt tacagtacgc tgtgcttcca acaccgtttg
    44521 ctgaaaacgc tttctaaatt catcagtttt gttacgtaaa gcctcatcag ataaggcctg
    44581 tgtttgttcc ttaaaacgat ttatgttttc cacaactggc ttgacttttt taatttcacg
    44641 ttggttcaca tcaccaatca gtctcgctaa gagtcctcgc atgtaaattc ctccttttac
    44701 accaagcaaa taaaaggaac cctacggttc ctttcagaag ccaaacttat tttaccatta
    44761 attcaagcca agtacaagaa tagcgcccct gtcaggctgg ggttaacatt ttgcgttgga
    44821 taaagtagaa gatgccggcc acaataaaaa tgtcacccat gctaatcacc tccgcgacgg
    44881 ggaagtaggg agggcggaga ggaataatat ctgccaaaaa gaagagccgc gttgttgctg
    44941 tcagtaaatt atgcgtgcca gcagggtctc cttgcagatt ggcaaaagac atggccacgg
    45001 cggaaactgg catcgtaccg ccattgatgg caataactaa aaagtttaag cagctgccag
    45061 ccgtaaatag ttttaagcca ggcagctgta agttttgcag cagggcataa aaaagcagca
    45121 gataagccac aatctgtaac caggccatat caatggcccg tactgctaag acccttacca
    45181 ggaggcgtaa caagagggct aaaattaccc acggtaaccc tttgagcccc aagtcgtgga
    45241 gtctttccag cttgccgccc cggagtttag ccatgattac tgccagaact acccctactg
    45301 caatcattca atttctccct tacgccgcag tactttgaga aaggctttga caatctcttc
    45361 actaaactga ctattgctgc agcgctgcaa ttccgccacg gcctcagcct tggtcagtgg
    45421 tgccctgtag atgcgctcgg aagtcatggc gtcataagca tcggctacgc ctatcatttt
    45481 agcacccaaa gatatttctt tttctgttaa cccatcggga taaccacttc catcgacccg
    45541 ctcatggtga tggcgtacaa tatcagcatg ttcaccgata aacttaatat tactgataat
    45601 ttcagcgccc acagctgagt gttccattac taaatgatat tcttcgtcgg agagacgact
    45661 tggtttattg agaatatgtt caggaacacc aatttttcct atgtcatgca ttaaagcaat
    45721 atattcaatt tgctctaaat aatcttccgg caaatgtaat tcttcgccaa tccacactgt
    45781 atatttagcc actcgttcag aatgtccacg cgtataatgg tccttggcat caatcgcttt
    45841 agtcagtgct ttaatcgtac tcatgtattg attacgcata tccatataca tgataaaagt
    45901 atgtcgtgct accattaacg ggacaattag cagcaaaaca cccggtattg ccatactatt
    45961 aaagacaacg gcgattagta caccgagcgg gcagttagat aaataattag gtaccgacca
    46021 tttaaagcta gtcaaccaaa cgcccataaa ggaaaccttt tgcgaaaaag tcacagcaag
    46081 catgaccaag gtagtattaa taaaaaagaa gacaaaggta cttacgagaa tggggacaat
    46141 atcatgggac aaatttaaac gtccattgat gccgccggtt agtataaagg catgtcctgc
    46201 caagccagtg gccaaggcca tctgggaagc attaaaaagc attttatacc aagattcacg
    46261 aaaacttttc aagctccgga tgtttaaaac cgtgcttaag cccgcccagg ctgctgccgt
    46321 tggtccaaat agcataatta aagccaagtc gacagcaaag tttacggaaa cagcactgtt
    46381 gccgcgcggt aaaggaacat ctaactgatc aagcataata tgtaagatta caaaaaagaa
    46441 aaaagagact gaaaacatct gtcttgttgg caaatggaag aataaaaaag taattgctgc
    46501 aatcgatact acggaaatat aaattttact ggctaaaggt tctttgctaa aagacaagat
    46561 gattcctccc gaatttaaaa aaaaggaccg accgtttaaa ttaaatccac ttgtaagctg
    46621 caccaccagc aagtacgagt gctgccaaag cagcaactgc gcgtaaaatc tttgccatta
    46681 gttgattgtc ccccttcacg gttatctctg tcgtggtccg ctagttaacg acaggcaaac
    46741 cgcttcgctc ggggaaaaga atgtcggtcc ttttttaaaa ctgatgattt aaatatgatt
    46801 aaagctcaag aggaacacgc ctattaatcg ccgccagggt tgcacgcacg acggcatctt
    46861 tatcatcatg acgaatcaag gcgctgccga caagagtttc ttcacttatg tcatgcagca
    46921 aggtcagcgt cgtagcaata atcgtctggc tgcccaggga aaactgcgcc acatcttcaa
    46981 aaatcagggt agcaccagga gctaagaact gcttgattgc ttctgtcgtg gcttgcgcaa
    47041 aaagtcgtat accattacgg cgtgtattga caccactact gcgaccttgg caggtacgct
    47101 tgtccgcagc cagctccact actgcttcca attggctgcc ctgcaaagag taggaaatac
    47161 ctgtgaaatt aagccgcttt tcatgggcca atttttgtcc ttgattaagc tgtacaatag
    47221 aaaccttttt gtgatccagg tcgatgccat actcgacttg caataatgtc tcgatatcgc
    47281 gcacaatttg cttggcatta cgcccagctt cggccaacac atgtacctcg acaatttcgc
    47341 tttgctcccc tagcacaata ttaactgata aaacgtcacg aatgcgtttg atcaagcttt
    47401 cacattctat tagcctgatt tcttttactt ctttagctgt atcagccacg agcccccatc
    47461 ccttctaccc gtagttctgc gccaaccagt gggatgtcat tttttcaatc cccttagtct
    47521 tgtttctatt ttcgaccaag tctgtctaaa tcctgcctct ttaggaaaaa acctcttaat
    47581 tttgacaaga aaagcaagct cccgagataa tgtcaactgt gacaacagat aactcctcgt
    47641 cgggccaagc agggtacatt atttcagctg ctttagcgtg cgcttttcag tcggccttag
    47701 ctgcttccct cactgagtag tcaggataag tgctagtctc ggcgagtaag ctcctctacc
    47761 aaaacctgta cgccgcctgt aactaaagag tgaccaccta aaaccaccgg aatactgcac
    47821 tgctgagcgg cccgggtaaa ttcacgcaca aagggatcct gtactttttc aaacagtccc
    47881 agatctgaat aaaaacggtc agcgtctgca ggctctttac catgatgtgc aaataagacc
    47941 cgatcatcga ttaaagcggc agagacagtc tgcgcaaggt aagtaaaaaa gcgttcaggt
    48001 ccaatcttct ccagccagaa acctagcagc gacaccactt cattgcgctc cagccgtccc
    48061 agtgctttca tgccccgttc ttctgaaaaa acgcgtaatc taaccttgag atttttattc
    48121 agacgctcca tggctggggc cccaatccgg ccaatcatgg ctacatcggc atattgcccc
    48181 tctaaaactt tttttacttg ctgcaatttt tccgtaggca gcgataactg ctttaaagtc
    48241 tggcgtaagt tctctcctcc gaagggtgac gcagccagta ccagcaagtc ggtaggtgta
    48301 tcaatgtcga attgtgaacc taaagttatc ggcagcagca cttgtgtaaa tttagcatca
    48361 taacgcagca gtaaggctaa agcattatct gtagccgccg ccggaaaatg ctgcaggatg
    48421 ttcgcagggt taaaggcaat aatgtcagca gactgcacat tattagtgac aaaacattgt
    48481 tctttagcca aaacctgctg gcataaccat cgcagttccg ccacactgat taatggcaca
    48541 gcggcgcccc ccatacagag cacagtgcgt acgcggtaac gttcaattaa attgattaat
    48601 tgccggccaa actgaaattg cgacgggggg atattgttaa cttccacgat tacttttggc
    48661 agagaggcat aggctttaaa ttcaggatga ttagtggcca gaataatgcg ctttacctct
    48721 gggactgcga caagcttgtt taaattatcc cgcagcgccg catggcgtac tgtcaacagc
    48781 atctcctcga ccgggctttt tgcttcgtta ccttcaaaaa ttatgacatc taacatcatg
    48841 acaccaaccc tctatctttt ttaagaacta tcggctaaga ttactgaaaa aagccggcgg
    48901 ctgccgactt ttttcagtgg ataaacaaaa ttatcatact gtagaattac gcaggtcgtt
    48961 agctggggcg gagagcaaac gtatttattt taaggctggt tcaatcaagc catattttcc
    49021 gtcatgtcga cggtaaacaa cgtttatctc atggttgttt tcagcattta caaaaacgaa
    49081 aaaatcgtgc cccagtagat tcatctgtag aatcgcttct tctacaggca tgggtttcac
    49141 aacaaagcgc ttatttttaa ccacaaataa gtctttttct tcctcgatgg cgggtagctc
    49201 attcatagct ttaaggctgc cggtttgacg agacttacgg ttaattctgg ttttatactt
    49261 ggcaatctgc cgctcaagtt tatcgaccac ctgatctatt gaagcataca tatcattagt
    49321 tttttcttcc gcccgcagaa tcatgccgtt aatcaagact gttacttcta ccttatggat
    49381 accacgctct acagagagcg taacttgagc ctcggtatca taagcaaagt atttgtcaat
    49441 cttactgact ttcttttcag cataatcctt tagtgcttct gtaacctcga tgtttttgcc
    49501 acgcacgatc agtttcatta atcatcagct ccttcttgct ttattaataa tacttcgtgc
    49561 tttcttcgat ccagggtctc ctttatcttt atcttactct ggggactcat ctgaaaaagt
    49621 cctcctaaac atatatatat ttccttttcg accgttaata atcctgctca ggataaatta
    49681 acaaaatata atatctctta tgattgtcat aa
//
LOCUS       DASRMS010000034         1924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_611778, whole genome shotgun sequence.
ACCESSION   DASRMS010000034 DASRMS010000000
VERSION     DASRMS010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611778"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..735)
                     /locus_tag="VFC74_01505"
     CDS             complement(<1..735)
                     /locus_tag="VFC74_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24042.1"
                     /translation="MNVTAPTTNGSSYYYKVYTKATQNDYLWSAASTAYATLTTNFTA
                     PTAPTVKVNNATSAYILADSGTATLSWSGASAGTNNPIASYLIYVDGTLYGETPSTQS
                     SISIPAYSAGNHSWTVITKGSYSNSAASSAVIVYAYSTPAAPTSVTINDASSIYLAEG
                     NTATLKWSGAGAGTNNPISSYLLYVDDTFYGETPASVSSVNIPSYGGGEHKWKVITKG
                     AYSNSAASSTASCFTYTATGAPTSISL"
     gene            complement(1367..1888)
                     /locus_tag="VFC74_01510"
     CDS             complement(1367..1888)
                     /locus_tag="VFC74_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003409106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24043.1"
                     /translation="MALDKTGLTRFWQHIVARLGTKVDKVEGKGLSTNDFTTEEKNKL
                     AAIESGATKTIVDDALNTSSTNPVQNAAVANAVNDINNRLGESSISDQIAAYAAPISH
                     THNYAGSSSAGGAATSAEKLTAARAITLSGAVSGTANFDGSGDTNISTTFPENITIPG
                     TITANKVIGAVYM"
BASE COUNT          479 a          403 c          482 g          560 t
ORIGIN      
        1 aaggcttata ctagttggcg cgccggtagc ggtgtaggtg aagcaggaag cagttgagga
       61 ggctgcgcta ttagaatacg cgccttttgt aattaccttc catttatgtt caccgccgcc
      121 ataagatgga atgtttacag agctaacaga ggcaggagtt tctccataaa atgtatcatc
      181 aacataaagc aaataactac taattgggtt gtttgtacca gcacctgctc cgctccattt
      241 tagtgtggcg gtatttcctt cagcaagata tatgcttgaa gcatcgttta tcgttacgct
      301 agtgggagct gcaggagtgc tgtaagcata tacaataaca gcagaactcg ccgcggaatt
      361 tgagtagcta cctttggtaa ttacagtcca gctgtgattg cctgctgaat aggcaggaat
      421 agaaatagag gactgcgtgg agggtgtttc tccataaagc gttccatcaa catagattag
      481 ataactcgcg attgggttat ttgttcctgc ggaagctcct gaccaagaaa gtgtcgccgt
      541 gccgctatct gctagaatgt aagcggaagt ggcgttgttt acttttacgg taggagcggt
      601 gggcgcggtg aaattggtgg ttagagtcgc gtaggcggta gaagctgcgc tccagagata
      661 atcattctgc gtagcttttg tatatacttt gtagtaatac gaagagccgt ttgtggttgg
      721 cgcggttaca ttcacagaac cggaggtcgc gtttgtggac acttcggtta gatagctata
      781 tgaaccactc gcgctcgttg ctcgatgaat atggtagccc gttatcgcat tattcgttcc
      841 agcggccgcg cctgaccagg aaagttgaac ggaagcgcct ggagcgacat tagttgaagc
      901 gacgcttact gcagttggag ccgttggatt tgtataagca acatagtcaa ttacaagctt
      961 gatatcgctt atacctaatg ttgagctatg gctaccaagc ccctaatttt tactaccgta
     1021 ggctctgaac tgtagctcgc cgcttacagt cccgttccct gtaagtggta tatcaatact
     1081 attcgtcttt ccagtactca agtttgtata agttcccgtt tgattgtttt tatatctaaa
     1141 cagtgctgcg ccagttgaag gtgagcctgc tgaaaaagat agcttagcac tgttgattat
     1201 tgcgccagct gcaaggccag taacatcaaa actaatcgaa cgatttgctt gcgtaggatt
     1261 ttgagaaaaa tataacttcc catttgcgct gtttacctat acagaccaac gactgacaag
     1321 gctgacattt tcaaatgtgt atgtcaacgt agccatttat ttcacctcac atataaacag
     1381 ctccaatgac tttgtttgcg gtaattgtac caggaatggt gatgttttct gggaaggttg
     1441 tcgagatatt tgtatcccca ctgccgtcaa aattcgctgt accacttacc gcgccggaaa
     1501 gagtaattgc gcgagcagca gtaagctttt ccgcacttgt tgcggcgcca ccagcagaag
     1561 aagaacctgc gtagttgtga gtgtgagata tcggagccgc gtaagccgcg atttggtcgc
     1621 taatagatga ttcaccgagg cgattgttga tatcatttac agcatttgcc actgctgcgt
     1681 tctggacagg attggtagaa gaagtgttta gggcatcatc tacgatggtt tttgttgctc
     1741 cgctttcaat cgcggcgagc ttgttttttt cttctgtggt aaaatcattt gtagaaaggc
     1801 cctttccctc taccttgtca accttagtac caaggcgggc aacgatgtgt tgccaaaagc
     1861 gggttaggcc ggttttatct agggccataa gaaagacctc ctgttagatt atttgctcag
     1921 gttt
//
LOCUS       DASRMS010000035         1611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_74891, whole genome shotgun sequence.
ACCESSION   DASRMS010000035 DASRMS010000000
VERSION     DASRMS010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1611
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74891"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
BASE COUNT          421 a          393 c          310 g          487 t
ORIGIN      
        1 tgtaactttt gtaacttttc ttcgtaactt ttcaatgtaa cttttatccg taacttttgt
       61 aacttttgtt ttcgtgtaac ttttgtaact ttccggcccc cgggcctccg gcctccggta
      121 atatactatc attgctttat atggcaagcg ccttgggatt gatttattaa ttgatggctt
      181 ttcagcgcaa caaaaagccg gaagcgattg cctccggcct tgcgtgatta tcctgtttta
      241 ccatcctttt aattcattgc cactgatcta gtcagctctg ctagtatcat gatcggtaat
      301 gcaattatag caaataatat aatcatggtc tcgcctccgg aagtattctc tgataagttt
      361 catcatcgaa gtagtgataa taggtatacg gcttgcaagg accaaaggca ttctttgttc
      421 gagtagctgc gataacataa cagttgtgtt tatcgcctct gtaatctgtt atgaatacct
      481 tattttttag cagtctcata cgtgataaag tttccccaaa gaattttagc gtatcatgac
      541 tgaagaagta atgttccccc ccagcagttt caatcttatt ttttaattca taaaatgtca
      601 ttgttccaat ccccgatctc aaattttagc gcttccagcg tagtatctcg caggccgcgc
      661 agagtgttac tgtaaggctg aatgttactg tcgacgtcgc agtaaaatgc atagtatgtg
      721 cagcggtccc gcgacgccgt gttaaccttt gcatactcgc agcgcttgca gtcaccgccg
      781 caagccgtca atgtttttat actccgccgc agccgcgcgg cttctttttt tgttatcatt
      841 attttgttcc tttctgttcc cgtcatcctt cgcgccgtac caggtcaatg aaaacgagcg
      901 cgagtaattt tataaggtac catacaatca taattcgata tactcgcatc gtacgtcgcc
      961 cgtgacgtta tcccgtataa gtacaggata atacgcgagc tcgcccgcgt catattctac
     1021 tagccagtca tagagatcac tcaacgctgc atccacttcc cactcgttga cggttattga
     1081 tcgtacggtt acactcccat tattggtgac gtaatcgtgt acttccagcg tgtatcggtc
     1141 tgttccctgt tccatgttat tccctcgtct tctgttagtg tgacggcgat cgccgtccgt
     1201 ccgttattac ctacccatat actatcacat atatatgtta atgtcaacag ggcacagagc
     1261 cagggcacag agccacggca cagagccacg gcacagagcc agggcacaga gccagggcac
     1321 agagccaggg cactgggtac ccccccttgc taaagccctg gtattttacc tccccggccc
     1381 ctcaaccaaa ttctattttt tcacctacca catctatcta ttgacaatca catctatctc
     1441 tgttattcta aacatacaga aaaaaaaaat aaaaaaaatt acaaaaaaac caaggacaaa
     1501 caaaagctga caagcttaac agaaagggag ttgaccataa cgtgttcctt gagaggtcat
     1561 tggaagcgtt agataagatt aaagctgata tttatataaa tgctaaggca g
//
LOCUS       DASRMS010000036         4512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_209980, whole genome shotgun sequence.
ACCESSION   DASRMS010000036 DASRMS010000000
VERSION     DASRMS010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4512
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209980"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="VFC74_01515"
     CDS             complement(<1..577)
                     /locus_tag="VFC74_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HZK24044.1"
                     /translation="MFVMHIGYARVSTQDQNSDLQIDALKQAGCEQIFNEKKTGRTLQ
                     RPELEACLRSLRKGDTLVVWRLDRLGRSLKDLVETVSALEERGVGFKSLTESIDTTSA
                     GGRLVFHLFGALAEFERNLISDRTKAGLAAARARGRKGGRRPKMSAADVKKAAAMLQD
                     PSITKAEVAKHFDVSRVTLNASLKREGYPENP"
     gene            798..995
                     /locus_tag="VFC74_01520"
     CDS             798..995
                     /locus_tag="VFC74_01520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24045.1"
                     /translation="MQWTEEQYQALLNAIEKGGSTQGVIDMAADTTSRTPKSVVRKLK
                     KIGYLRNSGQSFLSKQGFVSL"
     gene            1508..1831
                     /locus_tag="VFC74_01525"
     CDS             1508..1831
                     /locus_tag="VFC74_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24046.1"
                     /translation="MFYVIEHFHIILPEKQEDLFRIIVIQTRPELNTTTNDVEINGLC
                     SEQAGADGRVMYANGEYETFQEAKNNAIVKEDRNPIQPDSKSAVAAYIVESEHKPKLT
                     SQQSS"
     gene            complement(2326..2559)
                     /locus_tag="VFC74_01530"
     CDS             complement(2326..2559)
                     /locus_tag="VFC74_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24047.1"
                     /translation="MNTKIQTLSDLVNANKSTENEQHGASLNKETLSRDKIAMLSDIV
                     KTAKEEVLSGKHKDYEFASLADLVAQHEHEKQN"
     gene            complement(2732..3430)
                     /locus_tag="VFC74_01535"
     CDS             complement(2732..3430)
                     /locus_tag="VFC74_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24048.1"
                     /translation="MILSYYNGDQGGYSFSPWNVSDGHFTLACMYAGFVECIPDDGSY
                     WYTQEQKGHELSDAAKFFADQHATRLSCVQNQKHHLGKAGELGNMDALLVLAESFGDP
                     SIFKCRHLHHNYPASIAVVAEEMGRDADAKYWFTVAADQGDAYAMRELIDKDECSLFD
                     SWKWIYLSKLVGEDLTLDKYYAVHEDGSDYDDGKGGALYAGGYGGVSINSLNAELDAA
                     AKLAAQKLYKKNWL"
     gene            complement(3869..4129)
                     /locus_tag="VFC74_01540"
     CDS             complement(3869..4129)
                     /locus_tag="VFC74_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24049.1"
                     /translation="MSTNAERLKRYREKMADNGFKRLSFYAAPELVELIAKERKPSEC
                     GGRTLERLLLGEAKPRPDYWTAAERAQKAARRAASGRQITKA"
     gene            complement(4322..>4512)
                     /locus_tag="VFC74_01545"
     CDS             complement(4322..>4512)
                     /locus_tag="VFC74_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24050.1"
                     /translation="WLERVAHLLQSTPEHLLQSGLIDDVDLLEQWRTEPRHAAALIRS
                     CWRPDHYQAPDKQEDSRP"
BASE COUNT         1211 a         1123 c          918 g         1260 t
ORIGIN      
        1 cagggttttc tggataaccc tctcttttta atgaggcgtt cagcgttact cgtgatacat
       61 caaaatgctt agcaacttcc gctttagtga ttgacgggtc ttgtaacatt gctgctgctt
      121 tttttacgtc tgctgctgac atcttcggtc tacgaccacc tttacgccct ctagctcttg
      181 ctgccgctaa acctgccttt gttctatccg aaatcaaatt gcgctcaaac tcagccaaag
      241 caccaaacaa atggaatacc agccgaccac ctgcactggt tgtatctatg ctctcggtta
      301 gagatttaaa cccaacacct ctttcctcaa gggcactaac cgtttccaca aggtctttca
      361 atgacctccc aagcctatcc agccgccaaa caaccagcgt gtcacctttg cgcaaactgc
      421 gtaagcatgc ttccagctct ggccgctgaa gggtgcgccc agtctttttc tcattgaata
      481 tctgctcaca gcctgcctgc tttaacgcat caatctgaag gtctgaattt tggtcttgtg
      541 ttgaaactct cgcataacct atatgcataa caaacatacc tttgctgtac gttgataact
      601 ttacatagtt tctttacgct aaacaagctg atttaaataa ctttcagcaa gcagccagag
      661 ggtaaagaaa acggtcgtta tctttacgtt gctttaacta gaggaaatag gctagctgac
      721 gattaaggag ctgcaagtta cagtgtcgat agtcgcaaag ggattatttt caaactatgt
      781 agcaatagag gtgataaatg cagtggacag aagagcagta ccaagcatta ctgaacgcaa
      841 tagaaaaagg gggtagcact caaggtgtca ttgatatggc agccgacaca acatcccgta
      901 ccccaaagag cgtggtgcgt aagctaaaaa aaatagggta cttgagaaac agcggccaat
      961 cctttttatc caagcaaggc tttgtttcgc tgtaaaaacc ttgagtcgca atacgctggc
     1021 agcacctgtc tcatgaaaaa accttcacca gcagaccgta taggcttaat gatgaaggtt
     1081 tcatttcaaa aggcgcaaga agcacactgc ctgccaagga aagtacattt tctatagctt
     1141 tacgagccta gaaattaagc attgagttta ggtctaaagg gaatatgtca ccttaaccat
     1201 gtcatgcaac ccatctggaa acaaacaaca atcaatgctt ctttgcctgt ggataactca
     1261 accatgcgtg gataagcctg tggatacgca aagttataca cgcataaatg ccacccccta
     1321 aagagggaaa gtacgcataa atgccacccc ctagtacgca taaatgccac ccccctaaac
     1381 ctgtaaggtt tgcctttaat cttttgacct ataaaacttc ctataattgg ctggcgcttt
     1441 ctgtggataa cttggtactt tgtctagtag caacaaaccg tgattgttat caaaaaggcg
     1501 actaacaatg ttttatgtta ttgagcactt tcatattatt cttcccgaaa agcaggaaga
     1561 tctttttaga attattgtca tccagacaag gccagagcta aacacaacta ccaacgatgt
     1621 agaaataaac ggtttgtgca gcgagcaagc gggagcagac ggcagagtga tgtatgcaaa
     1681 cggtgagtac gagacgtttc aagaagccaa aaacaacgcg attgttaaag aagacagaaa
     1741 cccaattcaa cccgacagca aaagcgctgt agccgcctac atcgtagaaa gtgagcataa
     1801 gcctaagctc acctcacaac agagcagcta ggtggctttc aaggcgctat agaggacatt
     1861 actgcggctc ttgacttgct aatcactgaa gcgcacttgc tgctaagcag tagttatccc
     1921 caacttccac agattcaact gcattttatt ttttaatctt attatttttt aagcttttag
     1981 cttctctgta agccccatac ttgctggcct acagcaatct aatatggacg ttttccggct
     2041 taatctggac ggattccggc caagcatgga cactttccgg cattgatctg taagccccat
     2101 gcttgctggc ctacagtaat ccaatctgga cggattccag ctaagcatgg acactttccg
     2161 gctaaatgtg gacatattcc ggcattgata tggacggatt ccggttgata attactgact
     2221 tatccactga cgtaaaggca aatgacgcca aaaatagtta gaaaatcaat taaatgttgt
     2281 gatgacaaag aagcagcact ggagggagca gtgctgcgca ttacattaat tttgtttttc
     2341 atgctcatgc tgagcaacaa gatctgcaag cgatgcaaac tcataatctt tatgttttcc
     2401 gcttagaact tcctctttag cagttttcac aatatcgcta agcatagcta ttttatcccg
     2461 agacaaagtt tctttattta agctcgcccc atgctgttca ttttcagttg atttatttgc
     2521 atttactaga tctgaaagag tctgtatttt cgtgttcatt tttatgtttt ccatggcgag
     2581 tttagttttc aatcggaagt aagttacgcg ctacggtacg atgcccgtag caacgaaaga
     2641 gcctcattaa cacttatgtt ccaagcttgc gctattctgc gcacaaggta catagttata
     2701 actgcttgct cggtcttacc tttttgatgt tttaaagcca atttttctta tacaatttct
     2761 gcgcagcaag cttggcagca gcatccaatt cagcattcaa cgaattaatt gatactccac
     2821 cataaccacc agcgtaaagt gctccaccct tgccatcatc gtagtcagaa ccatcctcat
     2881 gaacagcata atatttgtcc aaagtgaggt cttcaccaac gagttttgat aggtatatcc
     2941 acttccaact atcaaacaaa ctacattcat ctttatcaat taattcacgc atggcataag
     3001 catcgccttg atctgctgca acagtaaacc aatatttagc atctgcatcg cgacccattt
     3061 cttctgctac aactgcaata gaagctgggt aattgtggtg taagtgccgg cacttgaaaa
     3121 tggatggatc accaaagctc tcagctaaaa ctagaagggc atccatattg cccaactcac
     3181 cagccttacc aagatggtgc ttttggttct gcacacaact gaggcgtgtc gcatgttgat
     3241 ctgcgaagaa tttcgcagca tcgcttaact catggccttt ctgttcctgg gtgtaccagt
     3301 aactcccgtc gtcgggaata cactcaacaa atccagcgta catgcacgcc aatgtaaaat
     3361 gtccgtcact aacattccat ggactaaaac tatacccacc ttgatcaccg ttataataac
     3421 ttaagatcat cttgtttcct ttccgttgga tgaatggcct attttttaat cagcacaaaa
     3481 tagtcttgga ggctcttcta ggcaccaatt aaaagtctga aagcccctaa ccatggggct
     3541 ttgtgctttc taggggttta cgataccccc aaaaataccc ccaaattgtt ttataccccc
     3601 tgctttatcc ctcaactggg caactccaaa tcaatgtccg gtacttcacc aaggtcaatg
     3661 tctggtactt cccccagatc aatgtctggc agctccaaat caatgtccgg cacttcaaag
     3721 tctagcatcg ctggtgctcc ttttttatcg agtcagaaaa ctatgcggct tatgcctttt
     3781 ttatagacgg gaatttcccc cgcactgtta ccactatcag gcacgttcaa ggtgtccaat
     3841 atttccacca tcacgcggct ttaataccct aagccttggt gatttgacgg ccactagctg
     3901 cacgccttgc cgccttctgt gctcgctctg ctgctgtcca atagtccggc cttggctttg
     3961 cctcacccaa tagcaaacgt tcaagggtgc ggccgccaca ctcgctaggc ttccgttctt
     4021 tagctatcaa ctccacaagc tcaggcgctg cgtaaaagct caggcgctta aagccgttgt
     4081 ctgccatctt ttcgcggtag cgttttaatc tctctgcatt ggtgctcatg ttttgccctg
     4141 ctgttagccc ttgctgttgt gctgccattg tactgacttc aataaaacct tgggcgcgtg
     4201 cgtagtgact gggaaaaaac ctttgggcgc ccgcgcgtat tgttggcgaa actcgtcatg
     4261 ccggtattgc gtggctgccc aggcgattag tagtcgtgcc accggttgtc tacgggtgtg
     4321 ctcatggcct gctgtcctcc tgtttatcag gtgcttggta gtgatccggc ctccagcagc
     4381 tacgaatcag cgcggcggca tggcgcggct cggtacgcca ctgctccagc aaatcaacgt
     4441 catcaattaa gccggattgc agcaggtgtt ccggggtcga ttgcagcagg tgtgccacgc
     4501 gctccaacca ta
//
LOCUS       DASRMS010000037        18085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_344712, whole genome shotgun sequence.
ACCESSION   DASRMS010000037 DASRMS010000000
VERSION     DASRMS010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 18085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18085
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_344712"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1479)
                     /locus_tag="VFC74_01550"
     CDS             complement(76..1479)
                     /locus_tag="VFC74_01550"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HZK24051.1"
                     /translation="MRHQRKLLSLLLLFCMVMSLFTGTAFGNPEDTRKEIAELEKVIA
                     ELKKDIADREKRVVQLNSELTASEKRLEQTKVALAESEAVLAEKNAIFGERVRSAYMS
                     GSLSYLDVILDAESFGDLILRFVYLSRILGRDANIVSSLREEYATLQERRLAMETEQD
                     KLKDLRYQIEAEHKNLVDQAKNMQKLLAAAQGRLADELAATIPQAEVKPVYGVVLDNH
                     ASARPQHGLSQANVVYEYEVEGRITRYLALFASFPSKVGPIRSARQHNVTLALENGVR
                     FVHAGGSQDNINLIGALNVRHTDALTSSSASFYRDKARKAPHNLYVNLKELKQESRSQ
                     TVVVRPSHLSRQGKTGSSFSLSYSGSYGVSFKYVADQGYYHRYINGKQHRDGNGAAIQ
                     AKNIIVQYTPHYNDFAGRATANLVGEGAIDFYSQGQYFRGVWKKSSEGSPTRYYYADG
                     QEIERVFGQTWIQIVRN"
     gene            complement(1494..2378)
                     /gene="ftsX"
                     /locus_tag="VFC74_01555"
     CDS             complement(1494..2378)
                     /gene="ftsX"
                     /locus_tag="VFC74_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HZK24052.1"
                     /translation="MNINSLKYCLRESVVSLKRNFWLAVVTACIIAVSLAILGGFLLL
                     TVNAGQFIRDVEANVEIGVFLEAEVDVQAVEQQLQQLSGVETITFVSKDDGLDDFSAS
                     LGDASLLAGLKGSENPLPDAFRVKTTDASLVSTLTETIRAIPGVEMADYGEELVGRLL
                     KITNWLNKLFVGVSVLLALGAVFLIVTTIRLSVMARLEEIGIMKYLGASDWYIRFPFL
                     LEGMAMGWVGTLASTAALGLAYSKLATSLQQDAFAFFIRPVTALEQLAPIYLGILILG
                     TLMGGLGSLISVRKFLRV"
     gene            complement(2368..3057)
                     /gene="ftsE"
                     /locus_tag="VFC74_01560"
     CDS             complement(2368..3057)
                     /gene="ftsE"
                     /locus_tag="VFC74_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HZK24053.1"
                     /translation="MIEFKAVTKTYPGGGRPALADISLQIDLGEFVFLVGPSGAGKST
                     LIKLFFREQLPTSGEIFFDGKNIANLEKAALLQHRRQIGMVFQDFRLLKQKTVFENVA
                     FALEVLGKSSKEIQAEVPLALAKVGLAGKGSSFPGELSGGEQQRVGVARALVKNPLIL
                     LADEPTGNLDRETSWQLMELFETINQGGTTVIIATHAWDAVDRMQKRVISLENGALTR
                     DEERGSYRDEH"
     gene            complement(3292..5427)
                     /locus_tag="VFC74_01565"
     CDS             complement(3292..5427)
                     /locus_tag="VFC74_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HZK24054.1"
                     /translation="MTAEAKIVKRIAQELQLAPQRLSNAIALLDEGNTIPFIARYRKE
                     VTVGMTDEELRALADKLNLYRNLEKSRTDIIRHLTEQSVLTAELRQAIEKAVTATELE
                     DIYRPYRPKRRTRATIAREKGLAPLAEILLTGTAEPAAAAEQYLNVEQEVHTAAEALA
                     GARDIIAETASDEPQTRQEIRRLLFARALIISKAVNEVESPFEMYYNFSEPLAKVQPH
                     RILAMNRGEKEKLLTIKLEFDEETALQVLAKHYLPLPASTAAAEQVTLALKDGWKRLL
                     FPSLEREVRSELTAQAEEQALKIFKANLRGLLLTPPVKGKRILALDPGYRTGCKLACV
                     DETGRLLETTVIYPTPPHNKKAAGAAEVKRLLNTHSLNLVAIGNGTAGRESEAFIAEI
                     LAELEANIEYTVVNEAGASVYSASKLGQAEFPALDVAERSAVSLARRVQDTLAELVKI
                     EPRAIGVGQYQHDINQKRLTEVLDGAVEDVVNQVGVDLTTASAALLERVAGINKSIAA
                     NIVTYREEHGVFTSRHELKKVAKLGPATFKQCAGFLRLPEAENYLDRSAVHPESYVTA
                     KQLLKLMEISLAELGNMPKLPELNVKETAEKLKVGEPTLRDILIEFQKPGRDPREDAP
                     KPIFKKGVLRLEDLQEGLVLTGVVRNVVDFGAFVDIGVHQDGLIHISKLADKYVRHPL
                     DVVKVGDVIEVKVLSVDVQKKRISLESSL"
     gene            complement(5530..6090)
                     /locus_tag="VFC74_01570"
     CDS             complement(5530..6090)
                     /locus_tag="VFC74_01570"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HZK24055.1"
                     /translation="MKKSFGAKILMYPCPVVAVGTYDQADKPNLMAVAWSGVVNGNPA
                     SVSIAVRKATYTYGNLLHKQAFTLNFPSRKYLQEFDFTGKSSGRNTDKFAATGLTPVH
                     SDLIDAPLVEEFPVVLECKLIKHVELGLHTLFIGEIVDAKVDAECLTDQVPDTRKIDP
                     LAYIPGDTTYYTLGEALARPVHDKKS"
     gene            complement(6130..6843)
                     /locus_tag="VFC74_01575"
     CDS             complement(6130..6843)
                     /locus_tag="VFC74_01575"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HZK24056.1"
                     /translation="MTKLLSLKKELQQLVDPEKADFLPQFFKAYPGGYGEGDLFIGVK
                     VPDQRRVVKKYVQQLSLPEVIQLLQEPIHEYRQTALLLLVELYQQAENAAEEEKIVRL
                     YLEYTEHINNWDLVDCSAEKILGAYLFTRTKDTLYLLARSNDLWEQRIAVIATFYFIK
                     QGFFSDTLQIAEILLQHPHDLIHKAVGWMLREVGKRDYQVEYDFLKRHYQVMPRTMLR
                     YAIEKFPPAVRQQFLKGTI"
     gene            7000..7968
                     /gene="uvsE"
                     /locus_tag="VFC74_01580"
     CDS             7000..7968
                     /gene="uvsE"
                     /locus_tag="VFC74_01580"
                     /inference="COORDINATES: protein motif:HMM:TIGR00629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009650 - UV protection [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UV DNA damage repair endonuclease UvsE"
                     /protein_id="HZK24057.1"
                     /translation="MLIRFGFVAMSTVLENASPSQTVTWKTYQKIRETSTDAAIEKLL
                     RVARQNLKNSLRLLQYCQANFVQVYRFSSKIIPLATHPEVEWNYLQDLQPELQALGEY
                     IITNKLRVTFHPDHFIVLNSPREVVFKNAVADLAYHSLLLTALQQDDNAKLVIHVGGS
                     YGNKETALERFMENWSLLPTGAAKRVILENDDRTYTANETLTLCEKLQVPMVLDVHHD
                     WCNHEPGRDLTEIYARFVGTWQATPLPPKIHVSSPKCTTAPRSHHNYVDAAAVYLVLK
                     QLQRYTSTLDVMVEAKQKDQAMLRLVEDLGRLAGVKLLNRATLVIE"
     gene            complement(7995..8435)
                     /locus_tag="VFC74_01585"
     CDS             complement(7995..8435)
                     /locus_tag="VFC74_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF188 domain-containing protein"
                     /protein_id="HZK24058.1"
                     /translation="MKIIIDADACPKSVLAMCREMGGQHQIEVWTVASFNHRIISDQH
                     FVVGNAPQETDIKVLNLAQLGDLVVTQDWGLAAMLMAKGVYCLAPSGKEYREETIDFL
                     LETRETLAKVRRSGGRTRGPKKRTAIDDQRFKAVLKKLITKADN"
     gene            8563..9786
                     /locus_tag="VFC74_01590"
     CDS             8563..9786
                     /locus_tag="VFC74_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HZK24059.1"
                     /translation="MLEQMLVKYAELVIKLGVNLQKGQTLVISTPLECAPFIRTLTKA
                     AYAAGARNVAFFWRDEQLDKIKYLYAPEDVFTEFAEWQREFYLSYAKQGAAFLSIAAE
                     DPELLQDVDHTRIAAAQKTSSTKLSEFRDRTMNNQNAWCVISAPTEGWARKVFPDCEE
                     PVAKLWQAILHTVRADLPDPLAAWHEHQNTLQKRLQLLNGYRLQTLHYTNSLGTDLTV
                     ALPENHLWQGATELTQEGVQFIPNMPTEEIFTLPQRTGVNGRVYSAMPLVYHGNLIDD
                     FVLTFKDGRVWDATAQAGAKHLHNLLEVDEGARYLGEVALVPYDSPISNTNILFYNTL
                     FDENASCHLAFGRAYPGSLADSAQLSKEELLVRGVNDSLIHVDFMIGTADLNITGTTA
                     AGEEIAIFRQGNFAL"
     gene            complement(9850..10755)
                     /locus_tag="VFC74_01595"
     CDS             complement(9850..10755)
                     /locus_tag="VFC74_01595"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK24060.1"
                     /translation="MPENSNLSPRELVQWVLENGVTDRIPKGELCITDGAIRKVMRVP
                     EAGFTEKKAFAETMGLDIYSLSPQYPQQEKSLPTKKEYLWPELKNWTVDSPYFIFAVL
                     DGAFEWGMRLLGFQEFCSNLRSPLVMEALVEQVENLNFYMIEKLAAEGVNGFILADDI
                     AHQQGLFARPQVFQEFFIPSLARQVEQILRLDLPVFYHSDGNYRAVISDIINTGVSGL
                     ECLEKTAGMHIKELQALLGPQVCLWGHLDTDDLEAAAQDASLLTPLLDNTLALAQGKR
                     FILGTTSGLFEGMQLEVLQELYASV"
     gene            complement(10907..12748)
                     /locus_tag="VFC74_01600"
     CDS             complement(10907..12748)
                     /locus_tag="VFC74_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZK24061.1"
                     /translation="MTKHSVSFPGGLQLEVETGATLKEVMNDAGINFDFPCGGRGRCG
                     KCLVRVTAGVGEPLAEEKKKLTAEELTDGIRFACLTEVQSEMTVEFMSDKNIQHRILL
                     SSLNRAAKVEPHLTKRYIEVDPATFTDQRTDWQRIKDGLAAQGNDSDVTGKPVAVLRT
                     VPDLLRDAKNKVTAVVYNQEIRGLEAGNTSEKMYGIAFDIGTTTIAGFLMDLYTGEEL
                     SVASALNPQTQFGADVISRIAHANQSPAGLADLYRVVTQTINKLIGEAAEKAGVLRDD
                     VYAVSIVGNTCMHHLFLGINPREVALAPYVPVISENLEVEPQALNLEINPAGLVFVLP
                     NIAGFVGADTVGVLLATELDETDEIKLVVDIGTNGEMALGNKDRMFACSTAAGPAFEG
                     AQISSGMRGATGAIDHVTFGDELKYSVIDDVKPLGICGSALLDIVAGMVEVGLLDERG
                     RIMKPENVTHPIGQKLKNRLIEQDGFLAFLVADASETNGKKITVTQKDIRELQLAKGA
                     MAAGIRILMNNYGIETDQIQEVLLAGAFGNYLNPHSACVIGLIPAELEERINMVGNAA
                     GAGAKLALLSSSEYQRSANAARNVEFIELGSDPSFTATFARTMRFTR"
     gene            complement(12802..13500)
                     /locus_tag="VFC74_01605"
     CDS             complement(12802..13500)
                     /locus_tag="VFC74_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK24062.1"
                     /translation="MVIPVYTQIVEAIKAKIDNGELKPGDAIDSENTLCKEFGASRMT
                     IRKGLGILVQDGYIYSIPGKGNFVKKPDLTKYTIYYDEMSNTINSVDRTRLLEVNIVM
                     PDDNLADNLQISKRKNVISIKRLFFTEGNPVAYDVKFLLYHRGMPIVEKELQHATFSE
                     MLAKTTPAFAIKKELLIYAEIPDEKIKHYLNIYNELPVLAVEQKLLTKDNKPMGMGIT
                     YYRGDYIKLSGTNQ"
     gene            complement(13503..14171)
                     /locus_tag="VFC74_01610"
     CDS             complement(13503..14171)
                     /locus_tag="VFC74_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795094.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK24063.1"
                     /translation="MANFFKLKEELINAVQELDEDRVLQLSEEALCLGVEPLLLMEAI
                     NAGMLAVGQLYEEKTYFIADLIMAGLIFKEVLGLEEMTTLFQIERQRKNGKLVLGTVH
                     GDLHDIGKDIFRGLMEANSFEVIDVGVDVPKEAFVKKVIEHRPGILGLSGVLTYTVEA
                     MKEVVDALAEAGLREQVKVIVGGGHLTPASCAYIGADSYANDALVGVNCCLRWAEISM
                     QGAE"
     gene            complement(14415..15221)
                     /locus_tag="VFC74_01615"
     CDS             complement(14415..15221)
                     /locus_tag="VFC74_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HZK24064.1"
                     /translation="MIIIGEKINGTIPKVGRAIAEKDEAYIRELAIKQAEAGASFIDI
                     CASTAEEIEVETLTWLMNIVQDAVDTPICIDSPNPKMIEAVFKHARRPGLLNSVSEEG
                     NKAEIIYPMIEGTDWQVVALTCDNKGIPADVQTRVDITKSLVEKAAKYNITPDRIHVD
                     PLVMALSADNGSMTTFIESMTKIKELYPTIKITSGLSNISFSMPLRKVINATFLSLAI
                     YAGMDSAIMDPLNRDMMATLLATEALLGRDRFCRNFSTAYRKNKIGPKKD"
     gene            complement(15782..16600)
                     /gene="larE"
                     /locus_tag="VFC74_01620"
     CDS             complement(15782..16600)
                     /gene="larE"
                     /locus_tag="VFC74_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013239319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HZK24065.1"
                     /translation="MTKTAKKLADLQQILRASSGAVIAYSGGVDSTFLARIAQDVLQD
                     RALMVMINSVLTPASEQQAAAALAAKLGFSFQVITANVLKNEDITENSPQRCYYCKKA
                     LFAELLQVAENNKMADVFDGSNFDDQYDFRPGARARQELGIRSPLQEAALTKAEIRLL
                     SQAYSLPTWDKPALACLASRFPYGERLTKEKLAMVEQAEEYLHSLSFLQVRVRHHGLI
                     ARIEVLPQQTPKIVTLSQQITAHFKALGYTYITLDLSGYRMGSMNEVLPGKLSC"
     gene            complement(16710..17102)
                     /locus_tag="VFC74_01625"
     CDS             complement(16710..17102)
                     /locus_tag="VFC74_01625"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013485528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HZK24066.1"
                     /translation="MDVYTRLLEQAQIARGQAYAPYSHFTVGAALLTAEGEIITGCNV
                     ENVSYGLTVCAERVATFTAMAAGKKKLIGLALVADLPEPPTPCGACRQVLAEFAPQMW
                     ILCANLQGRQRIFRLAELLPEAFAEFNS"
     gene            complement(17116..>18085)
                     /locus_tag="VFC74_01630"
     CDS             complement(17116..>18085)
                     /locus_tag="VFC74_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF881 domain-containing protein"
                     /protein_id="HZK24067.1"
                     /translation="FLATGFIYLNNHLDPVHEGFAAKQEAAKELVDYNRRLSKNLGVD
                     SRAQVREYLSRFNYEIEVAANEEELSKAILTYASRTQEKILQEYDTRQRETVLVLINQ
                     DALVQQIKEEKRLTVKLVRGEGVQTEPANVLSSETLQKIAETVTLGEQAQDLVIEVEI
                     ENGSGQLMVPLNAADQISSLNKDLDSLRVTVHDLRSQAGFAEMTGAGVIVKIYDNTDG
                     YTNDAIIHETDVRDTINELFAAGARGVAVGNQRLTVSSAIRCVGPSILVNDERIAVNP
                     VVIQAVGDPDVLASGLDIIRITLQVSRELTLEIEKTDHLTLPAYTR"
BASE COUNT         4903 a         4410 c         3587 g         5185 t
ORIGIN      
        1 gggtaccgtc agcgcggtac cctgccactg cttacttatt ttatgccgaa acgactgtca
       61 caggtgggac aattattagt tacggacaat ttgaatccaa gtttgtccaa aaacacgttc
      121 gatttcctga ccgtccgcat agtaataacg agttggtgaa ccctcactgc ttttcttcca
      181 aacgccacgg aaatactgac cttgtgaata gaaatcaatg gccccctcac cgactaaatt
      241 agccgttgcc ctacctgcaa agtcattata gtgcggcgta tattggacga taatattttt
      301 agcttggatc gccgcgccat taccatcacg atgctgcttg ccgtttatgt aacgatgata
      361 gtagccttga tctgcaacat atttgaagct tacaccatag ctgccactat aacttaagga
      421 aaatgagctg cctgttttac cttgacgact taaatgagat ggtcgtacca cgactgtctg
      481 tgagcgtgac tcctgtttta actcctttaa atttacataa agattgtgag gtgctttgcg
      541 cgccttatcg cgataaaaac tagcgctaga tgatgttaag gcatctgtat gtcgaacatt
      601 aagggcccca atcaggttga tattgtcctg actgccgcca gcatggacaa aacgtacccc
      661 attttccagt gctaaagtaa cgttatgttg gcgagcgctg cggatcgggc caactttact
      721 gggaaaagaa gcaaaaagtg ctaaatagcg agtaatgcgg ccttccacct catactcata
      781 tacaacattg gcctgggaca gtccatgctg tggtctggca gaggcatggt tgtcaaggac
      841 gaccccatac acgggcttta cttcagcctg cggaatggtt gccgccagtt cgtccgccaa
      901 cctgccttgt gccgccgcca aaagtttttg catatttttt gcttgatcga ctaaattttt
      961 atgttctgct tcaatctgat agcgcagatc ctttaattta tcctgctctg tttccattgc
     1021 caatctgcgt tcttgtaaag tagcatattc ttcacgtaaa gaagacacaa tgttagcatc
     1081 tctgcctaaa attcggctta aatagacaaa acgtaagatc aaatcgccaa aactctcggc
     1141 atctaaaata acatctagat aagaaagact gcctgacata taggcactac gtacccgttc
     1201 tccaaaaata gcatttttct ctgctaaaac agcttccgat tccgctagcg ctacttttgt
     1261 ttgttccagg cgtttttcag atgctgttag ttcgctgttt aattgaacaa cccttttttc
     1321 tctgtcggca atgtcttttt ttaactccgc aattaccttc tctaattcag caatctcttt
     1381 tctcgtatct tcaggattac cgaaagcagt acctgtaaaa aggctcatta ccatgcaaaa
     1441 cagcaacaaa aggctgagta gtttacgctg gtgtcgcata gtcattctcc ctcctaaacg
     1501 cgtaggaact tgcgcacaga aattaagctg cccaagccac ccattaacgt ccctaagatc
     1561 aagataccaa gataaatcgg tgccaactgc tccagcgcag ttacaggtct gataaagaaa
     1621 gcaaaggcat cctgttgcag cgatgtcgcc agcttactat aggctagtcc taaagcggct
     1681 gtcgaagcta aagtccctac ccaacccatc gccattcctt ccagcagaaa aggaaaacgg
     1741 atataccaat cgctggcacc taaatatttc ataatcccaa tttcctctag acgagccatc
     1801 acagaaagcc gtatagtggt gacgatcaga aaaactgcac ctaaagccag taagacactg
     1861 acaccgacaa acaatttatt aagccaattg gtaatcttaa gtagacgtcc aaccagctcc
     1921 tccccataat ccgccatttc cactccgggg atagcacgga ttgtctctgt cagcgttgag
     1981 acgagggaag catctgtcgt tttgacacga aaagcatcag gtaaagggtt ctctgaacct
     2041 tttaaccccg ctaacaatga ggcgtcacct agggaggcac taaaatcatc taaaccatca
     2101 tctttagaaa caaaggtgat agtctccacc ccagacaatt gctgcagttg ttgttcaact
     2161 gcctgcacat caacctcagc ttctaagaag acgccgatct ctacgtttgc ttctacatcg
     2221 cgaatgaatt gtcccgcatt aacggtcagc agtaaaaatc cgcctagaat cgctaaggag
     2281 acagcaataa tgcaggccgt cactacagct aaccaaaagt ttcgttttaa tgaaaccacg
     2341 ctttcgcgta agcaatattt aagtgaatta atgttcatcg cgataactcc cccgttcctc
     2401 atcccgtgtc agtgcaccat tttccaggga aattactctt ttttgcatac ggtctaccgc
     2461 atcccaagca tgggtagcga taatgactgt agtgccgccc tgattaatag tttcaaacaa
     2521 ctccattaac tgccaagaag tttcccgatc caagttgcct gttggttcat ctgctaatag
     2581 aattagagga tttttaacta aagcacgagc cacaccaacg cgctgctgct ccccacctga
     2641 caattctcca gggaaagaac ttcccttgcc tgccaagcca accttagcca gagcaagtgg
     2701 tacctctgcc tgaatttctt tgctcgactt acccaatact tccagcgcga aggccacatt
     2761 ttcaaagaca gtcttttgtt ttaagagacg aaagtcctga aaaaccatgc cgatttgtcg
     2821 gcgatgctgc aaaagagctg ctttttccaa gttcgcaata ttcttgccgt caaaaaagat
     2881 ttcgccactg gttggtaatt gttcccgaaa gaataacttg atcagtgtac tttttcccgc
     2941 gccgctgggg ccaacgagaa acacgaattc accaagatct atctgcagtg agatatcggc
     3001 caatgcaggt cgaccaccac caggatatgt ctttgttaca gccttaaact ctatcaaact
     3061 tcagcacccc tttatggtta aacctttata cagatttcga cctttttcca aataatcctc
     3121 ttaacagcaa ctttttgcaa gcaaaaaaac ttactctttt agtttccacc tgctagcctt
     3181 ttatcctgcc acctgcttac ttactgagtg taaagcaaaa ttgtttatta gtaaaaaaaa
     3241 agcggtctcc ttgctacagc aaggggccgc ttttggttcc accttttcgt cttacaaact
     3301 tgattctagg gagatgcgtt ttttctggac gtcgactgaa aggactttaa cttcgatcac
     3361 gtcgcctacc ttgaccacat ctaacggatg gcgcacatac ttgtcagcta gtttggagat
     3421 atgaataagc ccatcttggt gcacgccgat atccacaaaa gcaccaaaat caaccacatt
     3481 acgcactacg ccagtcagga ccaggccttc ctgcaaatcc tccagccgca gaactccttt
     3541 tttaaagatt ggtttggggg catcttcacg gggatcacgg cccggctttt gaaactctat
     3601 taagatgtcc cgcagagttg gttcgccgac cttgagcttt tctgctgtct cttttacatt
     3661 tagctccggg agttttggca tattgcccag ttcagccagc gaaatctcca ttagtttcag
     3721 cagctgttta gctgtgacgt aggattccgg gtgaactgca ctacggtcaa gatagttctc
     3781 cgcttccggc aggcgcaaaa acccggcaca ctgcttaaaa gtagcaggtc ctagcttggc
     3841 tacttttttc agctcatgtc gactggtaaa aacaccatgt tcttctctgt aagtcacgat
     3901 gttggcagca atacttttgt taatgcctgc gacccgctcc aagagcgcgg cagaggctgt
     3961 cgttaaatcg acgccaactt ggttcacgac gtcttccact gcgccatcca atacttcagt
     4021 caatcttttt tgattaatat catgctggta ttgccccacc ccaatggcgc gaggctctat
     4081 tttgaccaac tctgccaggg tatcttgtac ccgccgggct aaacttacag cactgcgctc
     4141 ggctacatcc agagcaggga actcggcttg tcctaatttt gatgctgaat aaacactagc
     4201 tccagcttca ttgaccacag tatattcaat attagcttct aattcagcta atatttctgc
     4261 aataaaggct tcactttcac ggccggcggt accgttgcct atggcaacca ggttcaaact
     4321 gtgcgtgttg aggagtcttt taacttcggc agcgcctgca gcttttttat tatggggagg
     4381 cgtaggatag ataaccgtag tctccagcag tctgcccgtt tcatcaacac aggctaattt
     4441 acaacctgtg cggtagcctg ggtctaaagc taaaatacgt ttacctttca caggtggcgt
     4501 gagtagtaag ccacgtaagt tggctttaaa aatcttcagg gcttgttctt cagcttgtgc
     4561 tgttaattca ctgcgtactt ctctttccag cgagggaaac aagaggcgct tccagccatc
     4621 ttttaaagcc agagttactt gttctgcagc tgctgtggaa gctggcagcg gcagataatg
     4681 ttttgccaga acttgcaggg ctgtttcttc atcaaattct agtttgatgg tcagcagttt
     4741 ctctttttca ccacggttca tcgccaggat gcgatgaggc tgcacttttg ctagtggttc
     4801 actaaagttg tagtacatct caaacggact ctctacttca tttaccgctt tactaatgat
     4861 cagcgcgcgc gcaaagagta agcgtctaat ttcctgccgc gtctggggct catcagaagc
     4921 tgtctcggcg ataatatcac gtgctccagc taaggcctct gctgccgtgt gtacttcctg
     4981 ctccacattc agatactgtt cggctgctgc agcaggctca gcggtgcctg tgagcagaat
     5041 ctctgctagt ggcgctaaac ccttttctcg cgcgatggtc gctcgtgtgc ggcgtttagg
     5101 acgataagga cgatagatat cttccagttc tgttgctgtg actgcttttt cgatggcctg
     5161 tcttaactct gcagtgagca cactctgttc tgtcaaatgt ctaataatat ctgtccggct
     5221 tttttccaga ttacgatata aatttagttt gtctgccagg gcacgtaatt cttcatctgt
     5281 catgccaact gtaacttcct tgcgataacg agcaataaaa ggaattgtgt ttccttcatc
     5341 caatagtgcg atagcattgc taagacgctg aggcgccagc tgtaattcct gggctattct
     5401 ttttacaatt tttgcttctg cggtcaatat aaatcctccg ttcaaatgtt tgtactgcta
     5461 gccttttatg ctgtttaggc aaaaaaatag gccaacccac gatcacagtt agcctagctt
     5521 gtggtatcct taggactttt tatcatgcac gggccgtgct agtgcttcac ccaaagtata
     5581 ataagtggta tcaccgggaa tataggctag tgggtcaatt tttcttgtgt ctgggacctg
     5641 gtcagtcagg cattcagcat caactttggc gtcaacaatc tcgccaataa aaagtgtatg
     5701 cagacctaat tctacatgtt taatcagttt acactctagg actacaggaa actcctcgac
     5761 gagcggtgca tcaattaaat cactgtgtac cggcgttaaa cctgtggccg caaatttatc
     5821 agtattacgt cccgatgatt tgccggtgaa atcaaactcc tgcaaatact tacgtgaagg
     5881 gaagttgagg gtaaatgctt gcttgtgcag caaattgcca tatgtatagg tggctttacg
     5941 aactgcaata gaaacactgg ccgggttgcc attgacgaca ccgctccagg ctacagccat
     6001 cagattgggc ttatctgctt gatcataggt acccaccgcc accaccggac acgggtacat
     6061 caatattttt gccccaaatg attttttcat ctccacacct ccagagaata ttgtttattc
     6121 taacttatgt tagatcgttc cttttaaaaa ctgttggcgc actgcagggg ggaatttttc
     6181 aatagcgtaa cgcagcattg tgcgcggcat tacttgataa tgccgcttta agaagtcata
     6241 ttctacctgg taatcacgtt taccaacttc ccgcaacatc caacccacag ccttatgaat
     6301 taaatcatgg ggatgctgca ataatatttc agctatctgt agtgtatcac tgaaaaagcc
     6361 ttgtttgata aaataaaaag tcgcgatgac cgcaatcctt tgttcccaca gatcattaga
     6421 acgagctaaa agataaagtg tatcttttgt tctggtaaaa agataggcgc ctaaaatttt
     6481 ctcagcgctg caatccacta aatcccaatt attaatatgc tccgtatact ccaaataaag
     6541 ccggacaatc ttctcctctt cagctgcgtt ttctgcctgt tgatataact ccactaaaag
     6601 gagaagtgct gtctggcgat attcatggat tggttcttgt agtaattgta ttacttctgg
     6661 taaagataat tgctgtacat atttttttac aactctgcgt tggtctggca ccttaacgcc
     6721 aataaaaagg tcaccttcac cgtagccacc gggatatgct ttgaagaatt gcggcaaaaa
     6781 gtctgctttt tctgggtcaa ctagttgctg tagttctttt tttagtgata aaagctttgt
     6841 catctttttc actcccagat gaagttgctt acttcagtat tcttctgcaa gcactgcatt
     6901 atatcctttt cagatgaaac taattattgt ttgcagcatc cagaacaggg aacgatattt
     6961 agtaaagctt aaacacataa acaaaattag gtgaaaaata tgctcattcg ttttggcttt
     7021 gtcgccatgt ccacagtgct ggaaaatgcc tcgccttccc aaactgtaac ctggaaaacg
     7081 taccaaaaga taagagaaac aagtacggac gcagccatag aaaaactttt acgcgttgct
     7141 cggcagaatt taaaaaattc tttacgctta ctgcagtact gccaggcgaa ctttgtgcaa
     7201 gtataccgtt tttcctccaa aatcatccct ttggcaacac atcccgaagt agagtggaat
     7261 tatctgcagg atttgcagcc cgaactacag gcccttggtg aatatatcat cacaaacaag
     7321 ctgcgggtta cctttcatcc tgatcacttt atcgtgctca attcgccgcg agaagtcgtt
     7381 tttaaaaacg ctgtcgcaga tttagcatac cacagtcttc ttctcactgc cctgcagcaa
     7441 gacgataacg ctaaattagt aatacatgtg ggcggcagct atggtaataa ggaaactgct
     7501 ttagaacgat ttatggagaa ctggagtcta ttgcctaccg gcgctgccaa gcgagttatc
     7561 ctggaaaatg atgatcgaac ttataccgcc aacgaaacct taaccctttg tgaaaagcta
     7621 caagttccaa tggtcttgga cgtccatcat gattggtgta accacgaacc aggacgagac
     7681 ttaacagaga tatatgcacg ttttgtgggc acttggcaag caacaccctt accacccaaa
     7741 atacatgttt ccagccccaa atgtacaaca gcaccgcgta gtcatcataa ctatgtcgat
     7801 gctgcagcgg tttacctagt acttaagcag ttacaaaggt acacatctac cctggatgtg
     7861 atggtggagg ccaaacaaaa ggatcaggct atgctccgcc tcgtcgaaga tttaggccgc
     7921 cttgccggtg taaagttact taatcgagcc accttagtta tcgagtaaaa cccaaacagc
     7981 attagcgact ttgctcagtt gtcagcctta gtaataagtt tttttaacac agccttaaaa
     8041 cgttgatcat ctatggccgt tcttttcttg ggacctctcg tgcgcccgcc agatcgccgc
     8101 actttggcta aagtttctct tgtttccagc aaaaaatcaa ttgtctcctc acgatactct
     8161 ttaccactgg gggcaaggca gtaaacacct ttggccataa gcatggcggc caagccccaa
     8221 tcctgggtaa cgaccagatc gcctagctga gcaagattaa gaactttaat gtctgtttcc
     8281 tgtggagcgt taccgactac aaaatgctga tcggatatga tccgatgatt aaaactagca
     8341 acagtccaca cttctatctg gtgttgcccg cccatttccc ggcacattgc cagtacactt
     8401 ttggggcagg catcggcatc aattataatt ttcatagcag cctcccgagt ccctcatttc
     8461 cagtgcagcg cagactattt tcccaggtaa ttgacaatga ctggtataat ctcacaaatt
     8521 cgtttacaat aagaattgta tcatagaaag gggaggttta gtatgttgga acaaatgtta
     8581 gttaaatatg cagaactggt aataaaactt ggtgttaatc tacaaaaggg gcaaacactg
     8641 gtgatttcta caccactaga atgtgctccg tttatcagaa ctcttaccaa ggcggcttat
     8701 gctgctgggg ccagaaatgt tgccttcttt tggcgagatg aacagctaga taaaataaag
     8761 tatttatatg caccagagga tgtttttacc gagtttgctg agtggcaaag ggaattttat
     8821 ctctcctacg ctaaacaagg cgctgctttt cttagtattg cggctgagga tccagaattg
     8881 ctgcaggacg tagatcatac acgcattgct gctgcgcaaa agacatctag tactaagcta
     8941 agtgagttca gggacagaac gatgaataac caaaacgcct ggtgcgtaat ctccgcacca
     9001 acagagggct gggctcgtaa agtgttccct gactgcgaag aacctgtagc taaattatgg
     9061 caggctattt tacataccgt gcgggcggat ctgcctgatc cccttgctgc ctggcacgaa
     9121 catcaaaata cattacaaaa aagattacaa ctgttaaatg gctatcgttt gcagacccta
     9181 cactatacta acagtctcgg taccgattta actgttgccc tgccagaaaa ccatctgtgg
     9241 cagggtgcta ctgaattaac acaagagggc gtccaattta ttcccaacat gcccacggaa
     9301 gagattttta cacttccgca aagaacaggt gttaacggtc gtgtttatag tgccatgccg
     9361 cttgtttatc acggtaattt aattgatgat tttgtgctga cctttaagga cgggcgagtt
     9421 tgggatgcta cagcccaagc aggagcaaag cacttgcata atctgcttga agtggatgag
     9481 ggtgcgcgtt acctaggaga agtggcactg gtgccctatg attcacctat ttccaacacc
     9541 aacattcttt tctacaacac actctttgat gaaaatgcct cctgtcacct ggcattcggc
     9601 cgcgcttatc ctggcagtct cgccgatagt gcccagctca gtaaagaaga attgttggta
     9661 cgcggtgtca atgattcact aattcacgtt gactttatga tagggacagc tgatttaaac
     9721 attaccggca caaccgctgc cggcgaagaa attgccatct ttaggcaggg aaattttgcc
     9781 ttataaccaa aagcgggcga agacaattcg cccgcttttg atatgacagc tcaaacaatc
     9841 ccacaccggc taaacactgg cgtaaagctc ttgcagcacc tcaagctgca taccttcaaa
     9901 aaggccgctg gttgtaccca ggataaatct ttttccctgg gctaaggcca gcgtattatc
     9961 caataatggg gtcaacaaac tggcgtcttg ggcggcagct tctaaatcat ctgtatctaa
    10021 atgcccccag aggcatactt gcggtcctaa tagcgcttgc agctctttta tgtgcatgcc
    10081 tgctgttttc tccaggcatt ctaaaccaga aacgcctgtg ttaatgatgt cacttataac
    10141 agcgcgataa ttaccatcag aatgataaaa cacgggtagg tccagcctta gaatttgttc
    10201 tacctgacga gccaggctgg gaataaagaa ctcctgaaac acttgcggcc tggcaaacaa
    10261 gccctgctgg tgcgcaatat catcagccag aataaagcca tttactcctt ctgcagccag
    10321 cttttctatc atataaaagt ttaaattctc tacttgctca actaaagctt ccatcactag
    10381 aggtgaacgc agattactac aaaactcctg aaaacccagt aagcgcatcc cccactcaaa
    10441 agcaccatct agcacagcaa agataaaata aggtgaatct actgtccaat ttttcagttc
    10501 tggccaaaga tattcctttt ttgtcggcag actcttctct tgctgaggat attgggggga
    10561 gagcgaataa atatctaagc ccatagtttc tgcaaatgct tttttctcag tgaagccggc
    10621 ttctggaaca cgcatgactt tacggatggc accatcggta atgcacagtt cacctttagg
    10681 tattctatct gtgacaccat tttccagaac ccactgtact aattcacggg gtgataaatt
    10741 actattttct ggcattcact tcgcctcctt atcagctcta attgccatca acagccctag
    10801 acctgctgta aacagcagat attattaact agcgcaaaga aagctaccaa taggcagctc
    10861 cccttgcggt atatttccga tgcggcagca ccacacacgt tacgtgttaa cgggtaaaac
    10921 gcatcgttct agcaaaagtg gcggtgaatg acgggtcgga gcccaactca ataaactcca
    10981 catttctagc agcatttgct gaacgctgat actcactgct ggaaagcaag gctaattttg
    11041 caccagctcc agccgcattg cctaccatat tgatacgctc ttctagctca gcagggatca
    11101 ggccaattac acaggcacta tgtggattca ggtagttgcc aaaggctccg gctaagagga
    11161 cttcctgaat ctgatctgtt tcaatgccat aattattcat gagaatcctg ataccagcag
    11221 ccatcgcacc tttggctaat tgtagttcac gaatatcttt ttgcgttacc gttatttttt
    11281 tgccgttcgt ttcactggca tcagctacca ggaaggctag aaaaccatct tgttcaatca
    11341 agcggttttt taatttctga ccaatcggat gggtgacatt ttccggtttc atgatccggc
    11401 ctcgctcatc cagcaaaccc acttctacca tgccagcaac tatatccagt aaagctgaac
    11461 cacaaatacc cagaggtttt acatcatcga tgacactata ttttaattcg tcaccaaaag
    11521 taacatgatc aatggcacct gttgctcctc gcatgccgga gctgatttgc gcaccttcaa
    11581 aggcagggcc ggcagctgtg gagcaagcaa acatgcgatc tttattaccc agcgccatct
    11641 caccgttagt accaatatcc acaactagct taatttcgtc agtttcatcc agctccgttg
    11701 ccaaaagcac accgacagtg tcggcaccaa caaaaccagc aatattaggc agcacgaaaa
    11761 ctaggcctgc tggattaatt tccagattca aagcttgtgg ttctacttct aaattctcac
    11821 taatcacagg aacataggga gctaaagcta cttcccgtgg attaattcca aggaataaat
    11881 ggtgcatgca agtgttgccg acaatagata ctgcataaac atcgtcccgc agcacaccag
    11941 ctttttctgc ggcttcacca atgagcttat taatagtttg cgttactaca cgatacaaat
    12001 cagctaaacc agcaggactt tgattggcgt gggcgatacg ggaaataaca tccgcaccaa
    12061 attgagtttg tgggtttaag gctgaagcaa cgctgagctc ttcgcctgta taaagatcca
    12121 tcaagaaacc ggcgatggtc gtagtaccaa tatcaaaggc aatgccatac attttttcag
    12181 aggtattccc agcttctaaa ccacgaattt cctggttata aacaacagct gtaactttat
    12241 ttttggcatc acgcagcaaa tcagggacag tccgcagtac ggcaaccggc ttacctgtga
    12301 cgtcgctatc attgccttgg gcagccaaac catctttgat acgctgccaa tctgtccgct
    12361 gatctgtgaa tgtggctgga tcgacttcta tgtagcgttt tgtaagatgt ggttcgactt
    12421 tggcggctct gtttaatgat gaaagtaaaa tgcggtgttg gatgttttta tcagacataa
    12481 attccacggt catctcgctt tgcacttcag tcagacaggc aaaacgaata ccgtccgtaa
    12541 gttcttctgc ggttaacttt ttcttttctt cagccagagg ctcaccgaca ccagctgtca
    12601 cccgtactag gcattttccg cagcgccctc tgcctccaca gggaaagtca aaattgatgc
    12661 ccgcatcgtt catgacttcc tttaaggtag caccggtttc cacctctaac tgtaagccgc
    12721 caggaaatga aacgctatgt ttcgtcatac acatcacctt tcgcatgttc tctttatcta
    12781 aattccctca caaatgcttc tctattgatt tgtgccggat agcttaatat aatccccgcg
    12841 ataatatgtg atccccatcc ccatgggctt attatccttc gttaatagtt tttgctctac
    12901 tgcaagtacc ggcagttcat tatagatatt cagataatgc ttgatttttt cgtcgggaat
    12961 ttccgcataa atcagcaatt ctttttttat agcaaaagca ggagttgttt tagcaagcat
    13021 ttcagaaaaa gtggcatgct gtaattcttt ttctacaatc ggcataccac gatggtataa
    13081 caagaatttg acatcatagg cgacagggtt tccttctgta aagaaaagtc ttttgataga
    13141 aattacattt ttacgtttag atatttgtaa gttgtcagcc aagttgtcat caggcatcac
    13201 gatattaact tctaatagtc gcgttctgtc aacactatta atggtgttgc tcatttcatc
    13261 ataataaata gtatactttg ttaggtctgg ctttttaaca aagtttccct taccgggaat
    13321 agagtaaata taaccatctt gaactaaaat gccaagtccc ttacgtatgg tcatgcggct
    13381 ggcgccgaat tccttgcata aggtattttc cgaatctatg gcgtcaccgg gcttcagttc
    13441 cccattatca attttagctt taattgcttc gacaatttgt gtatatactg gaattaccat
    13501 aattattctg ctccctgcat cgatatctca gcccaacgta aacagcagtt aacgccaact
    13561 aaagcatcat tggcatacga atctgccccg atataagcac aacttgctgg cgtgagatgg
    13621 ccgccgccga caataacctt tacttgctcc cgcaacccgg cctctgctaa ggcgtcaaca
    13681 acttctttca tcgcctctac agtataggtg agcacaccac ttaatcctaa tattccgggt
    13741 ctatgttcaa taaccttttt aacaaaagct tctttgggaa catcaacccc cacgtctatc
    13801 acttcgaaac tattagcttc catcagacca cggaaaatat ctttgccaat atcgtgcaaa
    13861 tcaccatgta cagtacctag gactaactta ccattttttc tttgcctctc aatttggaac
    13921 agtgttgtca tttcttccag gcctaaaact tctttaaaaa ttaaaccagc catgattaag
    13981 tcagcaataa agtatgtttt ttcttcatac aattgaccta ctgccagcat acctgcattt
    14041 attgcctcca ttaacagtaa aggttcaacc cctaaacata atgcttcctc tgataattgc
    14101 aatactctat cttcatccag ctcctgaaca gcgttaatta gttcttcctt cagcttaaag
    14161 aagtttgcca tagctacctc cgtacttcat tataccataa ttccgttgtg aaaaagaaat
    14221 aaaggggcta cataaacctt ctgcagcccc acgcaattcc ccttaacccc tatatggaat
    14281 ggcaccttgt tagtttttgt taagcttaca gctgcaaagg actccttcgt ttgctaattc
    14341 gcctattact ttaatgaatg ctttgtaatt tatgctaacc ttgctgctct agcaagatga
    14401 aattactatt tatactaatc ttttttgggg ccaattttat ttttacggta cgcagtagag
    14461 aaattacgac agaaacggtc gcgtcccaat agtgcttcag tggcaaggag agtggccatc
    14521 atatctctat taagcggatc cataattgca gaatccatac ctgcataaat agcgaggctt
    14581 aagaatgtag cgttgatcac ctttctcaat ggcatactaa aggaaatgtt gcttagccct
    14641 gaagtgattt tgatagttgg gtataattct tttatcttgg tcatgctttc aatgaaagtg
    14701 gtcatagaac cgttgtccgc agagagtgcc atcacaagtg gatcaacatg aattctatct
    14761 ggggtgatat tatatttagc tgctttttct actaatgact ttgtgatgtc aactcgagtt
    14821 tgtacatcgg caggaatacc cttgttatca caggttaagg ccacaacttg ccaatcggtg
    14881 ccttcaatca tgggataaat gatttcagcc ttgttgccct cttcggaaac tgaattaagt
    14941 aacccaggac gtctggcatg tttaaatact gcttcaatca ttttagggtt aggactgtca
    15001 atgcaaattg gtgtatctac ggcatcctgc acaatgttca tcaaccaagt aagtgtttcg
    15061 acctcaatct cttctgcggt actagcacaa atatcaataa aacttgcacc agcctcagct
    15121 tgcttaattg ccagttcacg aatataagcc tcatcttttt ccgcaatcgc acgcccaacc
    15181 ttaggaatag taccgttaat tttttctcca ataataatca tgcgagttcc tcctcctttt
    15241 tttttagagc ttttcacatt cacaggtaac atggacttcc tgaatccaaa catcacactt
    15301 ttaccgacat cacgattgct gggctttcca cgcacagtaa tcgtacgaga aagccaggtt
    15361 gtttgtcttg cgtttttagc taaagaatgc agctccattt ccgtgtagtt agtgcgaact
    15421 cgccatcgaa tactctttag ttaattgcta ctcattagcc ataatgttat tcttagaagc
    15481 gttcccgttc ttttcaacat catgcagaga aatgaataga tttacaaaac aaaaccatct
    15541 tggctataca tgtacttact cttacccaca atataacatt ataggtgcat gctgtcaata
    15601 aagattcgcg tttgccgtgc ttactcttaa tccttaccgc caggtaaccg caccctgcaa
    15661 gtatcagtgt ccacatgcca agatgacaca gattagcttt caccctcttt atattcttct
    15721 tttattgcca ttatcctgcg ctagactggc ctcttttacc gataatcata attttctaac
    15781 ttcagcagga caacttgccc ggtaaaactt cattcatgct ccccattctg tagcctgata
    15841 aatctagtgt gatataagta tagcctagtg ctttgaagtg agcggtgatc tgctgagata
    15901 atgtgacaat ttttggcgtt tgctggggta agacttcaat acgtgcaatt agaccatggt
    15961 gacgaactcg cacctgcaaa aatgacagtg aatgtaaata ttcctcagcc tgctcaacca
    16021 tagcgagctt ctctttggtg agacgttcgc cataaggaaa acggctcgct aaacaagcca
    16081 atgctggttt gtcccaagtt ggtagagagt acgcttgaga aagtaggcgg atttcagctt
    16141 tggttagtgc cgcttcttgc agcgggctcc taatccctaa ttcttggcgt gcgcgtgcgc
    16201 cgggccgaaa gtcatactgg tcatcaaaat tactaccatc aaagacatca gccatcttat
    16261 tgttttcagc aacttgcagc aactctgcaa aaagtgcttt tttacaatag taacaacgtt
    16321 gcggcgaatt ttccgtaatg tcctcatttt tcagcacatt ggcagtaatt acttgaaagg
    16381 aaaagcctag cttggccgct aaagccgctg ccgcttgctg ttcggaagcc ggagttagca
    16441 ctgaatttat cattaccatc agcgccctgt cctgcaaaac atcttgagca attcttgcta
    16501 aaaatgtgct gtcgacccca cccgaatagg cgattaccgc accactactt gcccttaaaa
    16561 tctgttgtaa gtcagctagc ttttttgctg tctttgtcat tgtctgctcc tttcattgat
    16621 acctaagaaa catctgccca ctcccgccaa atagttgaac attgaagagc gtaaatcagg
    16681 ctgaccttaa gtaatggtca gcctgatttt taactattaa attctgcaaa tgcttccggc
    16741 agcaattcag caagtctaaa aatacgctgc cggccctgca gattcgcaca caaaatccac
    16801 atctggggag caaactctgc cagaacttgg cgacaggcgc cgcagggtgt tggcggttca
    16861 ggcagatcag ccaccagcgc cagaccaatc agttttttct taccagcagc catagcagtg
    16921 aaggttgcca cacgttcagc acaaaccgtt aagccataag aaacgttttc cacattacaa
    16981 cctgtaataa tctctccctc tgccgttagc agagcagctc ccactgtgaa gtgtgaatag
    17041 ggggcataag cctgccctct agcaatttgt gcctgctcaa gtaaacgggt gtaaacatcc
    17101 attttaagca ctcctttaac gcgtgtaggc gggtaatgtt aaatgatcag ttttttcgat
    17161 ttccagagtt agttcacgcg acacctgtaa agtaatacga ataatatcta gcccacttgc
    17221 caaaacatct gggtcgccaa cagcttgtat caccaccggg tttacagcaa ttctttcatc
    17281 attgacaaga attgaaggcc ccacacagcg aatagccgag ctaaccgtta aacgttgatt
    17341 accaacagct accccacggg cacctgcggc aaataattca ttaatagtat cgcgtacatc
    17401 agtttcatga atgatggcat cattagtgta gccgtctgta ttatcgtaga tcttaacaat
    17461 cactccagca cctgtcattt cagcaaaacc tgcctgcgaa cgcaaatcat gcacagtcac
    17521 acgcagtgag tcaagatctt tgttcaggga gctaatttga tctgccgcat tcaaaggcac
    17581 cattaactgc ccactaccgt tttctatttc aacttcaatt actaaatcct gtgcttgttc
    17641 accaagggtt accgtctcgg caatcttctg cagcgtttct gagcttaaaa cattcgccgg
    17701 ttctgtttgt acaccctcgc cgcgcactaa ttttaccgtc agacgttttt cttctttaat
    17761 ttgctgcacc agtgcgtctt gattaattaa gaccaagaca gtttcccgct gcctggtgtc
    17821 atattcctgc agaatttttt cctgcgtacg agaagcataa gtaagaattg ctttgctcag
    17881 ctcttcctca ttagccgcca cttcaatctc gtagttaaag cgagataaat attcccgcac
    17941 ttgagcacgt gaatcaacac caaggttctt tgacagcctt cgattataat ctaccaattc
    18001 cttagcagct tcttgtttag ccgcaaaccc ctcatggacg ggatctaaat gattattaag
    18061 ataaataaat cctgttgcta agaac
//
LOCUS       DASRMS010000038        16416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_815845, whole genome shotgun sequence.
ACCESSION   DASRMS010000038 DASRMS010000000
VERSION     DASRMS010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 16416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16416
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815845"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1835)
                     /gene="ade"
                     /locus_tag="VFC74_01635"
     CDS             complement(39..1835)
                     /gene="ade"
                     /locus_tag="VFC74_01635"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012343902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HZK24068.1"
                     /translation="MFAKKENKLADVSQHLVEVAMGRTPADLVILDAKLVNVHTAEII
                     PHTDVAIAKGRIALVGQASHTIGQETKVINAHGAYLTPGFLDGHIHVESSMVTVTGFA
                     QAVVPYGTTGIFMDPHEIANVLGLEGVRLMHEEGRNVPLRVFTTVPSCVPAAPGMEDA
                     GAAIGPAEVREALSWNGVVGLGEMMNYPGVLANDENVHAIIKETLSSKKVVTGHFAMP
                     AQDYNLQAYLAAGVTSCHESVTKEDTLAKLRSGMYTMLREGSAWHDIKETIRAVTEQH
                     LDTSYCLLCSDDTHPETILSRGHLNHVVRRAIEEGLNPIRAIQMVTINTARYFRCDQD
                     LGSIAPGKFADLLLISDLTRVKVTKVLLAGTLVAAEGKMLLDTPSPQYPDFARQSVRL
                     AQPLTAADFQITAPDRASEVTVRVMQIKEAQVTTQELLQTVPVENGYLHANSTSDLAK
                     VAVFERHHKNGSHALGFVSGFGFTHGAVASTVAHDCHNLLIVGTNDADMAKAGNVLAA
                     CGGGMVAVADGQVLSLVELPVAGLMSDRPVTEVAAAVAELEQAWKKLGCTLISPFMTM
                     ALLSLPVIPALRITNRGLVDVNNFSMTDLLVK"
     gene            complement(1848..2552)
                     /locus_tag="VFC74_01640"
     CDS             complement(1848..2552)
                     /locus_tag="VFC74_01640"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HZK24069.1"
                     /translation="MIEAIFFDLDGTLLDVDMDDFLDRYLLKLSTHLNFAEPKSFARN
                     LWASTGEMVNSTDPTLTNQDAFMQHFITWVEQPQNEIMQLIAEFYQQIFPTLQDTAGP
                     FPHTATTLTAARELQCPLVLATNPIFPLTAIQHRMTWAGLTTEDFSMITSYENMHFAK
                     PNPHYYLEIAQMLKVEPSKCIMVGNDLELDIRAAGQAGLKTFYIDAAATSFNQDKNSG
                     RLEDFPLYLESLSIER"
     gene            complement(2626..3585)
                     /locus_tag="VFC74_01645"
     CDS             complement(2626..3585)
                     /locus_tag="VFC74_01645"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HZK24070.1"
                     /translation="MTKTLQAAVDVGGTKIYTVLADEKQQIVARQKIATLAVAGPEGV
                     IEQLVAGVEQIMHDFQAQRNDLTAVGVCIAGFYDWQQRLLMHSPNLPGWSNVPLESIL
                     AEKFNLPVLVENDANAAAIGEARLGAGANQQNVVFVTISTGIGAGLVINGQLYRGHCG
                     LAGEFGHIIVQPDGLHCGCGQRGCLETVASGTAIARQAQKAILSRHPTRLRTLVKNPT
                     KVTAEEVFRAARLGDTLAQDIIREAVDYLGLAVVNIINLLNPAVVVLGGGVAQAVEDV
                     VVPLRKIVTECAISPAAKAVQLEHAALGEEAGVLGMLSLLQKL"
     misc_binding    3720..3935
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3987..5513
                     /locus_tag="VFC74_01650"
     CDS             3987..5513
                     /locus_tag="VFC74_01650"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HZK24071.1"
                     /translation="MTDKIIIFDSTLRDGEQTPGAKLNLAEKLKIAKQLAQLNVDVIE
                     CGFPISSPGDFKAVQRIAQEVTGPVITALARALKPDIDAVWEAIKEAERPRIHTFLSS
                     SPIHMQYQLRKDPVTVLKMGVEAVKYAKQYTADVQYSLMDATRSELDYLCRVVEAVIA
                     AGATVVNIPDTVGYAVPEQFGELISNIMNRVPNIDQATISVHCHNDLGLAVANSLVAI
                     ENGARQVECNINGIGERAGNAALEEIVVAMTIRNDYFHNYHHQIETQEIYKTSRLVSS
                     LMGIPIPVNKAVIGTNAFVHSSGIHQDGVLKNMETYEIINAELIGGKAAQMVLTARSG
                     RHAVRHQLQELGFTLQDTEFEEFFARFLELADKKKEVFREDLEALMNENITHTVPIYS
                     LEYLQTVSGSKSIPAAVVKLKKDQETFVETQTGAGPIDAAYNAIDKITGFQPRLENYN
                     LRGVTEGRDALGEVSIMLEYQDKTIVGRGTSTDIIEASVRAYLNGINKLLALGSNGNK
                     "
     gene            complement(5582..6178)
                     /locus_tag="VFC74_01655"
     CDS             complement(5582..6178)
                     /locus_tag="VFC74_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdE family protein"
                     /protein_id="HZK24072.1"
                     /translation="MDLQLWARAVEFHGHTCFGLAMGYHVATLALETLATYRAPDEQI
                     VAVVENDNCSVDAIQIITGCTLGKGNLIYRDYGKKVYTFFLRTTKKGIRITVQGPEMS
                     EYPELTELQAKVKANQATPEDKIALAQKQQEAILHYLNKPPTEVCTVSEAQDHLPARA
                     RMFNTLTCHFCGEEMMEPRARVKDGQFACIPCADLYLR"
     gene            6508..8112
                     /locus_tag="VFC74_01660"
     CDS             6508..8112
                     /locus_tag="VFC74_01660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZK24073.1"
                     /translation="MTWNSIIQLFGGLALFLYGMTLMASGMQKAAGDRLRKILEFLTT
                     KPIVAVLTGALATMLVQSSSTTTVMLVGFVNAGLMTLTQAVGTILGANIGTTITAQLV
                     SFKVSNLVYPAITIGGLLNFFGGRRSYRYFGQAILGFGILFLGMTVMSESMEPLRQNQ
                     FFINLMVSFGRIPILGVLIGAVFTGIIQSSSATTGIVIAMTMQGVLGLPAALAMTLGS
                     NIGTCVTALLACIGTGLSARRAAVAHLLFNVTGVVIALVFFNQFLDFVLLTGNSVTRQ
                     TANAHTLFNVINTLLFLPFLKQFVAVVTRLVPGEEAAIEMGAKYLDKHILRTPDIALN
                     GVRQECLRMAAQAREMVSEAMQYLIKGDKKIVQHVQQKEDLVDSLEKEITIYLAELAQ
                     HSLTQEQSREMSALMHVVNDLERIGDHAQNIIQLAEEKNEDRLPFSELAIDELQEMYE
                     KVDEMITMAIAAFAKNDMHLAGEVVDQDDIVDNIERSLRRSHISRINDQVCFPPSGVI
                     FLDVISNLERIADHSTNFAQVVLEDI"
     gene            complement(8409..8723)
                     /locus_tag="VFC74_01665"
     CDS             complement(8409..8723)
                     /locus_tag="VFC74_01665"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZK24074.1"
                     /translation="MKTLATYRYQRQQSLCKQQKFIRTALVLLVLTIVIGITFVNNNV
                     ALGETRLPQDIVVETGDTLWTLAQRYAPQRMDIRKYVRIIMEHNELTGPLVYPGQLLE
                     LP"
     gene            8864..9478
                     /gene="lexA"
                     /locus_tag="VFC74_01670"
     CDS             8864..9478
                     /gene="lexA"
                     /locus_tag="VFC74_01670"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HZK24075.1"
                     /translation="MIDLTPRQKQILEYIKQEVKTKNYPPSVREIGEAVGLSSSSTVH
                     AHLAKLEEKGLIRRDPSKPRAIALLEDDPAPGYIPEVVQVPLLGQVTAGEPILAEENI
                     EEYFPLPKMMVRNDNVFLLRVRGDSMINAGILNGDSVIVRQQNNANNGEIVVAMLDDE
                     ATVKRFYKEKDSIRLQPENPHYEPIRSRDIMVIGKVIGVFRELH"
     gene            complement(9557..11008)
                     /locus_tag="VFC74_01675"
     CDS             complement(9557..11008)
                     /locus_tag="VFC74_01675"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZK24076.1"
                     /translation="MKLASLLAGYPLKFTTVNLPTKIHGFSADSRKIKPGYLYICIRG
                     ENEDGHGFVQQAVEQGASVIVSQKFLQLSVPIILVEDTRHFLSFFADSFFNHPSQKIQ
                     ITGITGTNGKTTTAHYLHEIYQAAEQQSALMGTCGVKTRQQYRQTQCLTTPGAEELHN
                     ILWQLQLSGVQQLAMEVSSHALVQKRVEHCRFHAAIFTNLTREHLDYHQTMDDYFAAK
                     AHLFALLAAREGNAIINADDKWAIKLTKQIAAPFITFGLENKADVRAEKITSLTKGGS
                     VLNVASPWGKLSFTLYLPGLYNIYNALGAATAALMDNLTLQEVTKGLEALRVVPGRLE
                     LLPSPPGVQVYVDYAHTPDGLEKVLQTVTAYPHHRIILVFGCRGRRDRGKRPAMGVVA
                     DHYADIVVLTTDNPVEEDPETIAQDITSAMQKKPIFLADRQEAIHYALAAASDGDIVL
                     ITGKGREDYQLVGAATIPYSDLHAVESFVNSMH"
     gene            complement(11118..11759)
                     /gene="nth"
                     /locus_tag="VFC74_01680"
     CDS             complement(11118..11759)
                     /gene="nth"
                     /locus_tag="VFC74_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003411836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HZK24077.1"
                     /translation="MSSKEQAKQIIRILKEQNPHPATELNFQTPWQLLVATILAAQST
                     DKQVNKVTAKLFLDYPQPSDLAALTENQLAESIKTLGLFRNKSKHLVATARKIVKKYS
                     GEVPRTLAELQGLPGVGRKTANVVLANAFGIPAFAVDTHVFRVANRTGLATAKTPEQT
                     EKQLTNLIPREQWRDAHHWLILHGRYICTARKPQCPRCPIAKYCQTDKLNTTN"
     gene            complement(11764..14193)
                     /locus_tag="VFC74_01685"
     CDS             complement(11764..14193)
                     /locus_tag="VFC74_01685"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HZK24078.1"
                     /translation="MPDKQNLTKATIKVGGMSCAACANSIEKALQKREGIFEAVVNFA
                     AEKVAVTFAPQVISVADIHKVITDLDYEVIEESKARETKMVLPVTGMTCASCAAAIER
                     GLNKQAGVTEAVVNFATEKVTVQYDSSAVTREEIIRLIEGLGYGVIEAEVTENRSSDA
                     REEREAAELAARKRVLAFVMAITLVTETIMFLEYRQTFHIPYHNWIMLVAATPIVFWA
                     GFPTHRGAWKSLRHGSANMDVLISLGSLSAYAWGIAAFFIPDAVSFMGISSMIMAFHL
                     LGRYLEAIAKSKTSGAIRKLLELGAKTARIIVDGQEREIPVERVQVGDILVVRPGEKL
                     PVDGEIVEGHTTVDESMVTGESLPAEKVVGDLVVGATINKNGAIKFKATKVGKDTFLA
                     QIVRMVEEAQGSKAPIQELADKVTGYFVPVVVLIAAATFSGWLLFGGTNSLTHAVFAS
                     IAVLVIACPCALGLATPTAIMVGTGLGAENGILIKDAESLQTMRDVNAVIFDKTGTIT
                     KGEPTVTDLLTLGELTEEELLTIAASGETGSEHPLGQAIVQAAEEQSISLKEAQSFTA
                     IPGKGIEAVIEGQKVTMGNRKLMHEQGIDTAEVETQLAELEEQGKTAMLITQNGVLSG
                     IIAVADTVKEDSRRAIAALTKAGLTTAMLTGDNERTAQAIASQVGIKRILAQVLPDQK
                     EAEVRRLQAEGLTVAMVGDGINDAPALAQANVGIAIGTGTDIAIETSDITLIRGDLTS
                     VVTAISLSRATFKIIRQNLFWAFGYNILAIPIAASGRLNPAIAALTMAMSSVSVILNS
                     LRLRRQKIIVE"
     gene            complement(14451..15860)
                     /gene="ltrA"
                     /locus_tag="VFC74_01690"
     CDS             complement(14451..15860)
                     /gene="ltrA"
                     /locus_tag="VFC74_01690"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003964 - RNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0000373 - Group II intron splicing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HZK24079.1"
                     /translation="MRDTKKYVESRQLPKEGYLQEVRVELGGNAGARNISSTSEVDGN
                     GGNGYAGGLLEKILDRHNMNRAYKRVKANKGSHGVDGMTVDELLQYLKQNGNQIIQSI
                     RNGEYRPSPVRRVEIPKPDGGKRLLGIPTVLDRVIQQAIAQVLSPIYEKRFLDNSYGF
                     RPGRSAKQAVMKCKEYIEAGYNWSVDIDLAKYFDTVNHDKLMRLLSETIKDSGVLSLI
                     RKYLQSGVMINGVVMGTEEGTPQGGNLSPLLSNVMLHELDKELTKRGLKFCRYADDCN
                     IYVRSRKAADRVMASITRFIEEELKLKVNKAKSSVDRPWKLKFLGFSFYRKKGEIGIR
                     VHPKPVAKLKQRLKEITGRSNAMSMYQRMVKLRQIIVGWVNYFGIADMRGLAKSLDMW
                     LRRRVRMCFWKQWKKIKTKHNNLVALGAPGYKAWEHANTRKSYWRIAGSPILSKTITN
                     EYLKKLGLLSISERYSLVH"
     gene            16206..>16416
                     /locus_tag="VFC74_01695"
     CDS             16206..>16416
                     /locus_tag="VFC74_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24080.1"
                     /translation="MANGSHCKAHSGLSPPSYRPCRAHDKDGRPSVCPRCSNEELNGE
                     YCKICGTYIVNKCSAETDLDLNGNEY"
BASE COUNT         4338 a         3736 c         3635 g         4707 t
ORIGIN      
        1 agtttatcct gatttattta ctttacaaac tagttttatt attttaccaa aagatcggtc
       61 atagaaaagt tgttgacatc aacgaggcct cggttggtga tgcgtaaagc tggaattacc
      121 ggtaatgaaa gtaaggccat cgtcatgaaa ggtgatatta atgtgcagcc aagcttcttc
      181 cacgcttgct cgagctctgc aacagctgca gctacttctg tcacagggcg atccgacatc
      241 aggccggcta ctggcagttc caccaatgac agtacctgac catcggccac agctaccatc
      301 ccaccaccac atgcggctaa aacattgcca gccttggcca tatctgcatc attagtacca
      361 acgatcaaga gattatgaca atcgtgtgca actgtggacg ccacagcacc gtgcgtaaag
      421 ccaaagccgg agacaaagcc aagggcatgg ctgccatttt tatgatgacg ttcaaaaaca
      481 gcaactttag ctaaatcgct ggtagaattg gcgtgtagat agccattttc tacgggaact
      541 gtctgcagga gttcttgtgt agtaacttgc gcctccttaa tttgcattac acgcactgtt
      601 acttcactag ccctatctgg ggcggtaatt tgaaagtcag cggctgtcag tggttgggcc
      661 aggcgcacag attgccgggc aaagtccggg tattgtggtg aaggtgtgtc tagcagcatc
      721 ttgccctctg ccgcgactaa tgtgccggca agcagaactt tagtcacttt gactcgggtt
      781 agatcggaaa tgagtagtaa atcagcaaac ttacccgggg cgatactgcc taaatcctga
      841 tcacaacgaa aataacgggc ggtattaatg gtcaccattt gaatagcacg gatgggattt
      901 aaaccttcct cgattgcccg ccgcacaacg tgatttaggt gtccacggga gagaatcgtt
      961 tctgggtgtg tgtcatctga acaaagtaga cagtaggaag tatcaagatg ttgttcagtg
     1021 acggccctga tagtctcttt aatgtcgtgc caggcagatc cttcacgcag catcgtatac
     1081 ataccacttc ttaattttgc taaagtatct tcttttgtca ctgattcatg acaggaagtg
     1141 acgccagcag caaggtaggc ttgtagatta tagtcttggg ccggcatggc aaaatgcccg
     1201 gtgactactt ttttagatga gagtgtttct ttaatgatgg catggacatt ttcgtcattg
     1261 gctaaaacac cggggtagtt catcatttcc cctagaccga cgacaccgtt ccaggatagt
     1321 gcttccctaa cctctgcggg gccaatcgca gcgccagcgt cttccatgcc cggtgctgcc
     1381 ggtacgcatg aaggaacggt agtgaaaaca cgcagcggta catttctacc ttcttcatgc
     1441 atcagacgca ccccttctag gccgaggacg ttagcaatct catgcggatc catgaagata
     1501 cctgttgtcc cgtatggtac aacggcttgg gcaaatcctg ttacagttac catgctgctt
     1561 tcaacgtgga tatggccatc taaaaaacca ggtgtcagat aagcaccatg ggcgttaatt
     1621 acttttgtct cctgaccgat ggtgtgacta gcttggccaa ctaaggcaat acgccctttt
     1681 gctatagcta catctgtatg gggaataatt tcggcagtat gtacattaac taatttggca
     1741 tcaaggataa ctaaatcagc tggcgtgcgg cccatcgcaa cttctaccaa gtgttgggat
     1801 acatctgcta atttattttc ttttttcgca aacatgacaa tcctcctcta cctttctata
     1861 ctaagtgact ctaaataaag tggaaaatct tcaagacggc cactgttttt gtcttggtta
     1921 aagctagtag ccgctgcatc aatatagaaa gtttttaatc cggcttgacc agcagcgcga
     1981 atatctaatt ctaaatcatt gcccaccata atacatttgc tcggctctac tttaagcatt
     2041 tgtgcaattt ctaaataata gtgggggttc ggtttggcaa agtgcatatt ttcataggag
     2101 gtaatcatgc tgaaatcctc tgtagtcaga ccagcccaag tcatgcgatg ttgaatagct
     2161 gtcaagggga aaatggggtt tgttgccagt accaaggggc actgcaattc acgtgcagca
     2221 gtgagtgttg ttgctgtatg cgggaatgga ccagcggtat cctgtaatgt ggggaatatt
     2281 tgctgataaa attctgcaat tagctgcatg atttcatttt gtggttgttc aacccaagta
     2341 ataaaatgct gcataaaagc atcttgatta gtcaaagtcg gatctgtact attaaccatc
     2401 tcccctgtag aggcccataa atttctcgca aagcttttgg gttcggcaaa attgaggtga
     2461 gttgacaatt tcagcaaata acggtctaaa aaatcgtcca tatcaacatc tagtaatgtt
     2521 ccatctaaat caaaaaaaat agcttcaatc atttagatca cccctttccc atgcactttc
     2581 gacaagttgg ttgctatttc ctgtttttat agggttgttt gtacattata atttttgtaa
     2641 taagcttaac atccccagca cccctgcttc ttcccctaag gctgcgtgtt cgagctgtac
     2701 tgctttagca gcaggtgaga tggcacactc agtcacgatt ttacgtaatg gtacgactac
     2761 atcttcaaca gcttgagcaa caccgccgcc tagaaccaca acggctggat tcagcagatt
     2821 aatgatattt actacagcta aaccgagata atctactgcc tcccttatga tatcttgggc
     2881 cagagtatcc cccagccttg cggcccgaaa tacctcctct gctgtcactt ttgttggatt
     2941 tttaaccaaa gtgcggagtc ttgtgggatg tcgagaaagt attgcttttt gagcttgacg
     3001 ggcgatggct gtacctgagg ccacagtttc caagcaacca cgctgtccac agccgcagtg
     3061 taacccatct ggctgtacaa taatgtggcc aaactcacct gccaaaccac agtgaccgcg
     3121 atacaactgt ccatttataa ctagaccagc cccgataccg gtgctgatgg ttacaaaaac
     3181 aacattctgc tggttagcac cagcgcctaa acgtgcttcg ccgatggccg cagcattagc
     3241 atcattttct accagtacag gtaggttaaa cttttctgct aaaatgctct ccagtggtac
     3301 attcgaccaa ccgggcaaat tcggagagtg catcagcagg cgttgctgcc agtcgtaaaa
     3361 accagcaatg catactccga cagcagtcag gtcgtttcgt tgtgcttgaa aatcatgcat
     3421 gatctgctcg acgccagcaa caagctgctc aatcacacct tctggccccg ccactgctaa
     3481 agtagcaatt ttttgccgtg cgacaatttg ctgtttttca tctgccaata ctgtataaat
     3541 tttagtgccg ccgacatcaa ctgcggcttg cagcgtcttg gtcaatctaa tcgccgcctc
     3601 gttattattt tgaagctgtt accttcttac cagatagtag tattattcac gcttaacacc
     3661 aataaaatta atccttgact tttcgctgtg gtttgttatg ataaaaagaa attcgaaata
     3721 ttaaagtgat gatggggaat aaatgttgat actacagaac agagagccgt cgggtggtgt
     3781 gagacggtct gaagtccaac atgaactcac cccggaatgc cgatactgaa agataacaag
     3841 taagtctggc cgtctcccgc gttatgggat taaagcaggt acgtcatttt gacggactta
     3901 agtagagtgg taccgcggac cttttcgcct ctagggtgaa ggggtttttt gttatagaaa
     3961 ataatttaat ctaaggagga tttacaatga ctgataagat tattatcttt gattcaacac
     4021 tgcgcgatgg cgaacagact cctggagcaa agcttaattt agcagaaaag ctaaagatcg
     4081 ccaaacaact ggcccaacta aatgtggatg ttattgaatg cggtttccca atctcgtccc
     4141 caggggattt taaagcagta caaagaattg ctcaggaagt tacaggccct gttattaccg
     4201 ctttagcccg tgctctgaag ccggatatcg atgcagtttg ggaagctatc aaggaggcag
     4261 aacgtccccg cattcatacc tttctatcct catcccctat ccatatgcag tatcaactgc
     4321 gcaaagatcc cgtaactgtc ctcaagatgg gggtagaagc agtaaagtat gccaaacaat
     4381 atacagctga cgtccaatat tcactaatgg atgcaactcg tagtgagctt gattatctgt
     4441 gccgggtagt agaagcggta atcgctgccg gtgctaccgt agttaatatc ccggacactg
     4501 taggctatgc agtgccagaa caatttggcg aattaatcag caacattatg aaccgggtac
     4561 ccaacattga tcaggcaacc attagtgtgc actgccataa tgatttaggg ctagctgttg
     4621 ctaactcact ggtagctata gaaaacggtg cccggcaagt ggagtgcaat attaatggta
     4681 ttggtgagcg ggcaggcaat gccgccctag aagaaattgt ggtggcgatg acaattcgca
     4741 acgattattt ccacaactat catcatcaaa ttgaaacaca ggaaatttat aaaaccagcc
     4801 gtttagtgtc gagtctaatg ggcatcccta taccggtgaa taaggcagtt atcggtacta
     4861 atgcctttgt gcactcatcc ggtattcatc aggacggcgt gctaaaaaat atggaaacat
     4921 atgaaattat taatgcagaa ttgataggcg gcaaagcagc acaaatggtg ctaacagctc
     4981 gttccggacg acatgcggta cgtcatcaat tgcaagagct aggctttacc ctgcaagata
     5041 ccgaatttga agagttcttc gcccgttttc tggagttagc tgataagaaa aaggaagttt
     5101 tccgtgaaga tctcgaagca ttgatgaatg aaaatattac tcatactgta ccaatttata
     5161 gtttggaata tttacaaact gtgagcggct ccaagagcat accagccgca gtcgttaaat
     5221 taaaaaaaga tcaggagact tttgtggaga cacaaactgg tgccggcccc atagatgctg
     5281 cctataatgc cattgataaa atcactggtt tccagccgcg tttggaaaac tataatcttc
     5341 gcggggtaac ggaaggtcga gatgctttgg gtgaagtttc catcatgctt gaatatcaag
     5401 ataaaaccat tgtcggtcgc gggacatcca ccgatattat tgaagcttct gtgcgagctt
     5461 acctgaatgg cattaataaa ttgctggcac tgggcagtaa cggcaataaa taaaataatt
     5521 tgctatattc actgccggcc aattatattt ggccggcggt tttgttagta tcagtcttct
     5581 actagcgaag atataaatcg gcacagggga tacaggcaaa ttgaccatct ttgacgcgtg
     5641 cccgaggctc catcatctct tctccacaaa agtggcaggt cagggtgtta aacattcgtg
     5701 ctcgtgctgg tagatgatct tgagcttcgc tgactgtaca gacttctgtt ggtggtttat
     5761 tcaggtaatg aagaattgct tcctgctgtt tttgcgccag agctatttta tcttcaggag
     5821 ttgcttgatt agcttttact tttgcctgca gttcagtaag ctcgggatat tcgctcatct
     5881 cagggccctg aactgtgatg cggatgcctt tctttgttgt gcggagaaaa aacgtatata
     5941 cctttttccc gtagtcgcga taaattagat taccttttcc caaggtacaa ccagtaatta
     6001 tttgaatagc atctacggag caattatcgt tttcaaccac agcgacaatt tgctcatctg
     6061 gagctcgata tgtggccaaa gtttctaagg ccagtgtggc cacgtggtat cccatcgcta
     6121 agccgaaaca agtatgacca tgaaattcaa cggctcttgc ccatagttgt agatccatct
     6181 attcacaacc tttcgtgtta ttattatgac ataagtagca cctattatga atagtttctg
     6241 ctcattgccg gcaatttcct gccagcataa aaacatttgt gctggcaact gtcaattcac
     6301 gcttttgata ctagctgcct aaaaatatct gcgaaataat atgatacaca tgtagtctag
     6361 gccttttaaa atcagagcaa tcatcagacg ctacaagttt actttctgct tgtaaaggat
     6421 actttcatcc gctataatta ttgtggtgtt atgaccgaaa ataaataaaa atatctgacg
     6481 tattacgaca taatcgcggg aggatctatg acctggaact caattatcca attatttggt
     6541 ggcctggcgc tgtttctgta tggtatgacg ctaatggcct caggaatgca gaaagcggcg
     6601 ggagataggc tgcgcaagat tctcgagttt cttaccacga aaccgatcgt ggcagtgctc
     6661 actggggctc tagccacgat gctggtgcaa agcagcagta ccaccacagt gatgctagtt
     6721 ggttttgtta atgccggcct aatgacgttg acacaagcag ttggtaccat tcttggggcg
     6781 aatatcggta ctacgattac tgcacaatta gtatccttta aagtatccaa tcttgtctat
     6841 ccggctatca ccattggagg cttgttgaac ttcttcggcg ggcgccgcag ctaccgctat
     6901 ttcggacaag caattttagg cttcggtatc ctctttctgg gaatgacagt tatgtcggaa
     6961 agtatggaac cgctgcgcca gaatcaattc tttatcaacc tgatggtttc ctttggccgc
     7021 atccctattt taggggtatt aattggagct gtttttaccg gtattattca gtccagcagc
     7081 gccactacgg gtattgttat cgcgatgacg atgcagggtg tattaggtct tcccgcagct
     7141 ctagccatga ctttgggatc caatatcgga acatgtgtta cagccttgct cgcctgtatt
     7201 ggtaccggcc tgtccgcccg gcgggctgcc gtggcacatc tgttgtttaa tgtaactggc
     7261 gtggtgattg ctcttgtttt ctttaatcag tttctagact ttgttctcct cactggaaat
     7321 agtgttacaa ggcagaccgc caatgctcac accctgttta acgtgattaa taccctgctc
     7381 tttctaccct tcctcaagca gtttgttgct gtggtcaccc gtctagtacc gggggaggaa
     7441 gcagcaattg aaatgggagc taaatattta gacaagcata ttttgagaac accagatatt
     7501 gccctcaacg gggtgcgtca agaatgcctg cgtatggccg cccaggcccg tgaaatggtc
     7561 agtgaagcca tgcagtactt aattaaaggt gataagaaaa tagtgcagca cgtgcagcaa
     7621 aaggaagatt tggtagactc gttggaaaaa gaaatcacca tctatctggc ggaactggca
     7681 cagcattccc ttacacaaga acaatctcgg gagatgtcag ctttaatgca tgtagtcaat
     7741 gacttagagc ggatcggtga tcacgctcag aatattatcc agttggcgga agaaaagaac
     7801 gaagatcgtc tgcccttctc tgaactggcc attgatgagt tacaggaaat gtacgaaaaa
     7861 gtagacgaaa tgattaccat ggccattgct gcttttgcta agaatgatat gcatttagca
     7921 ggagaagtgg ttgaccagga cgatatcgtg gataatatag aacgttcttt acgtagatct
     7981 catatttcac gcattaacga tcaagtttgt tttcccccca gcggtgtgat cttcttagat
     8041 gtcatcagta atctagagcg cattgctgac cattcaacta actttgcaca agttgttttg
     8101 gaagatatct aagcttaaca tggtaaaagg ctaaattaag cccgggttgt cgccgggttt
     8161 ttttgcttta tagaaagcaa tataattcat aaaataaata caggcgttat cagtaaacgc
     8221 ctgtattttg cgattcgttg gttgtttccg gctgtgaggt atctcctgtt aagggaaaat
     8281 aatcaaacat tggatattcg tcaatttcgt cgagaaaata gtcttgatgg ttccatggta
     8341 actcttgcac catttgcatc acctcggtgt attttgcctt agcttaacct gaggtgctgc
     8401 agaatatgtt agggcagttc tagtaattga ccaggataaa caagcggtcc ggttaactcg
     8461 ttatgttcca tgataatcct gacatatttc ctgatatcca tacgctgcgg cgcgtaacgc
     8521 tgagccagtg tccaaagagt atcacctgtc tcgacaacaa tatcctgcgg taaacgtgtt
     8581 tcaccaaggg ccacgttatt gttaacaaaa gttatcccga tcacaatggt caagactagt
     8641 aaaaccagtg ctgtgcgaat aaatttttgt tgtttgcata aactctgttg tcgctgatat
     8701 ctataagtgg ctaacgtttt catcttaagc cccccaaaca tttgtttgtc tatatcttaa
     8761 cacgaacata tgtgcgtgtc aatacttacg gtaaaaaaaa cgaacgaatg tttgcaagat
     8821 ggtaaatata tgttataata caagaaaagt gaggtgctgc cggatgatag acttaacccc
     8881 gagacaaaaa caaattttag aatatattaa gcaagaagta aaaacaaaga attacccacc
     8941 ttcagtacgt gaaattggcg aagcagtagg cctctcttct agttccactg tacatgcaca
     9001 tctcgcaaaa ctagaagaaa aaggtctcat ccgccgtgat ccgagcaaac ctagagccat
     9061 cgctttactg gaagatgatc ctgctcctgg ttatatacct gaagtggtgc aggtaccgct
     9121 gctcgggcaa gtcacggcag gcgaaccaat tttagctgag gaaaacattg aagaatactt
     9181 ccccttacct aaaatgatgg tacgtaacga taatgtattc cttttacgtg tccgcggcga
     9241 cagcatgatt aatgccggca tcctcaatgg agacagcgtt attgtacgtc aacaaaataa
     9301 tgccaacaat ggtgagatcg ttgtagctat gcttgatgat gaagctacag ttaaacgttt
     9361 ttataaagaa aaggacagta tccgtttgca gccagaaaac ccacattatg aacctatccg
     9421 ttcccgagac attatggtga tcggtaaagt tatcggtgtc tttcgtgaac tacattaaga
     9481 aaatgctttc atctgcggat gaaagcattt ttctttcttt gcgtggtggt cgggagttaa
     9541 aaacttgcgc tgggttttag tgcatggagt tgacaaacga ttctaccgcg tggagatcgg
     9601 aatatggaat cgttgcagcg cctactagtt ggtagtcctc tctgccttta ccagttatta
     9661 agacaatatc accatcgcta gctgcggcta aagcataatg aattgcttct tggcgatcag
     9721 cgagaaatat tggtttcttc tgcattgcag acgtaatgtc ttgcgctatg gtttctgggt
     9781 cttcttctac cggattatct gttgttaaaa caacgatgtc ggcatagtgg tcggccacta
     9841 ctcccatggc gggacgttta ccacggtctc gtcggccacg gcagccaaat actagaatga
     9901 tacgatgatg gggataggct gttactgttt gcagtacctt ttccaaacca tccggtgtgt
     9961 gagcataatc tacgtaaacc tgcactcccg gcggtgatgg taataactct agtcggcctg
    10021 gtaccacgcg caaggcttca agtcccttgg tcacttcctg cagcgtgagg ttgtccatta
    10081 atgcagcagt ggcagcccct aaagcgttat aaatattgta caagccgggt aagtacagag
    10141 taaaggaaag cttaccccac ggtgaggcaa cattcaaaac agagccgcct ttggttaagc
    10201 tagttatttt ttcagctctc acatcagcct tgttctccag accaaaggtg ataaacggcg
    10261 cggcaatttg ttttgttaac ttgattgccc acttgtcatc agcattaata atggcgtttc
    10321 cttctcgcgc tgctaacagc gcaaagaggt gagctttagc tgcgaaataa tcatccatgg
    10381 tctggtgata atccaaatgt tctcgggtca ggttggtaaa gatggcggcg tgaaaacggc
    10441 aatgctccac tcttttctgc actaaagcat gcgaggatac ttccatggct agctgttgta
    10501 ctccactaag ctgcagctgc caaagaatat tgtgtaattc ttcagcacct ggcgtggtta
    10561 agcattgtgt ttgccggtat tgttgtcttg ttttaacgcc gcatgttccc attaaagcac
    10621 tttgctgttc tgcggcctga taaatttcat gtaaatagtg agcagtagtc gttttaccat
    10681 tagtaccagt aattccggta atctgtattt tttgactggg gtgattaaag aaagaatcag
    10741 cgaaaaatga gagaaaatgc cttgtatctt caactagaat aatggggacg gacagctgta
    10801 ggaacttttg actgacaatc acagaggcac cctgctccac tgcttgctgt acaaaaccat
    10861 gaccgtcttc attttcaccg cggatacaaa tataaagata gcccggtttt attttccgag
    10921 aatctgcaga aaaaccgtgt attttagttg gcaagttaac cgtggtaaat ttaaggggat
    10981 aaccggcaag gagtgatgcc aatttcatga ggtacctccg caacaacggt tttaataatc
    11041 taaattagct ttaccattgt cattattccc ttaacactgt caaaaattta tcagtgcagt
    11101 ttttcagcta acattaatta atttgtagta ttcaacttgt ctgtttggca gtatttagcg
    11161 atagggcagc ggggacactg tggtttacgc gccgtacaga tatagcgacc atgtaagata
    11221 agccaatggt gagcatcgcg ccactgctcc cgggggatta gatttgtcag ttgtttttct
    11281 gtttgctccg gtgtttttgc agtagctaaa ccggtacgat tggcaacacg aaagacatgt
    11341 gtatctactg caaatgcggg aatgccgaag gcatttgcca gtactacatt ggctgttttt
    11401 cgtcccacac ctggtaaacc ttgtaattca gctaaagttc gtggcacttc tccgctatat
    11461 tttttcacaa ttttccgagc agtggcgaca aggtgtttac ttttattgcg aaagaggcct
    11521 aatgttttta tgctctcagc cagttgattt tccgttaagg cagcaagatc agatggttgg
    11581 ggataatcaa gaaagagctt tgccgtgact ttatttacct gtttatcggt tgattgggcg
    11641 gctaaaatgg tagccacaag caattgccac ggagtttgaa aattgagctc cgtggcagga
    11701 tgtggatttt gttcttttag gatccgaata atttgtttag cctgttcttt actggacatc
    11761 agcttactcc acaattattt tttggcgtcg taaacgcaat gaatttaaga ttaccgaaac
    11821 agatgacata gccatggtta aggcagcgat agcaggattt aggcgcccag aagcggcgat
    11881 ggggatggct aaaatgttgt agccaaaggc ccagaataag ttttgccgga tgattttgaa
    11941 agtagcacgg gaaaggctga tggcagtaac taccgaagtt aaatcaccac gaattaaagt
    12001 aatatcagaa gtctcaatgg cgatatcggt gccggtccca atggcaatgc caacattggc
    12061 ttgggcgagt gctggtgcat cattaatacc atcaccgacc atggcaaccg ttaagccctc
    12121 tgcctgcaga cggcgtactt ctgcttcttt ttgatccggc agtacttgag ctaaaatgcg
    12181 tttgatcccc acttgggagg caatagcctg tgcagttcgt tcgttatcac ccgtcagcat
    12241 ggctgtagtg aggccggctt tcgttaaagc agcaatagct cgacggctat cttcttttac
    12301 ggtatcagct acagcaatga tcccgcttaa aacaccattt tgggtaatca acatcgctgt
    12361 tttaccctgt tcctctaatt ctgctaattg agtttctacc tcagctgtat caattccttg
    12421 ctcatgcatt aatttacgat tacccatcgt aaccttttgt ccctcaataa ccgcctcaat
    12481 acctttaccg ggaatagcag taaaggactg tgcttctttt agtgagattg attgctcttc
    12541 tgccgcttgc acaatggctt gccccagcgg gtgttctgaa cctgtttctc cgctagcggc
    12601 gattgttaac agctcttcct ctgttaactc acctagtgtt aaaaggtctg tgacagtggg
    12661 ttcacctttg gtgatagtac ctgttttgtc aaaaataaca gcatttacat cacgcatcgt
    12721 ttgtaaactt tcagcatctt tgatcagaat accattttca gcacctagac ctgtccccac
    12781 cataatagct gtgggggtag caagccccag cgcacaaggg caggcaatta ctagtaccgc
    12841 aatggacgca aagacagcgt gagtcaggga atttgtaccg ccaaacaaga gccagccgct
    12901 aaaagtagca gcggcaataa gtacgacaac aggaacaaaa tagcctgtta ctttgtcagc
    12961 tagttcctgg atgggtgctt tagagccttg tgcttcttcg accatacgca cgatttgtgc
    13021 tagaaaagtg tctttgccaa ctttggtcgc tttgaattta atcgcaccat ttttattaat
    13081 ggtggcgcca acaacaagat cacccactac tttttccgcc ggcagacttt caccggtaac
    13141 catagattca tcaactgtag tatgaccttc gactatttca ccatctactg gcagcttttc
    13201 accgggacgt actaccaaga tgtcaccaac ttgaacacgt tccacgggaa tttctctttc
    13261 ttgaccatca acaatgatcc gtgctgtttt agcaccaagc tccaatagtt ttcggatagc
    13321 gccggatgtt ttacttttag caatagcttc aagataacgt cctaagaggt gaaacgccat
    13381 aatcatgctg gagatgccca taaaggagac agcgtcagga ataaaaaaag ctgctatacc
    13441 ccaagcatag gccgaaagtg accccagtga aattaagaca tccatattcg cgctgccatg
    13501 ccgcagagat ttccaggctc cacggtgtgt ggggaaacca gcccaaaaga caatgggtgt
    13561 agcggccaca agcattatcc agttatggta aggtatatgg aaggtttgac gatattctaa
    13621 aaacatgata gtttcagtaa ccaaagtgat ggccatgaca aaggctaaca cgcgcttacg
    13681 ggcggctaac tctgccgctt cccgctcttc ccgggcgtcg ctgctgcggt tttctgtaac
    13741 ttcagcttcg atcaccccgt aacctagccc ttctatgagg cggatgatct cttcgcgggt
    13801 aactgcgctg ctatcatatt gcacagttac tttttctgtg gcaaagttaa caactgcttc
    13861 agtaacacct gcttgtttgt ttagaccacg ctcgatggcc gcggcacaag aggcgcaggt
    13921 catgcccgtt acgggcagca ccatttttgt ttcccttgct ttagactctt caattacttc
    13981 gtaatctaaa tcggtgataa ctttgtggat gtctgccaca gaaattacct gtggagcaaa
    14041 agttaccgca actttctcgg cagcaaagtt gacgacagct tcaaagatgc cctctctttt
    14101 ttgcagtgct ttctcaatac tattagcgca ggccgcacaa gacatccctc cgacttttat
    14161 ggttgcttta gttaaattct gtttatctgg catcttaacg cctccttata cccctcccag
    14221 gggggggtgg atatatataa tttatcacct tgccttgctt ctgtcaatta gtttctacta
    14281 aaaatgatgc ctcatgcgtt ttgtcaactt tagtgcagca aaaaagccta agataagtcc
    14341 acggtgtaat agaagcaatt taaggatcga gtaggaggct acccattaat tgaatagccg
    14401 tcctctcaca ccaccgtacg taccgttcgg tatacggcgg ttcaatagaa ttagtggact
    14461 agtgaatatc tttcactgat tgacagaagt cccagcttct taaggtattc attggttatg
    14521 gttttcgaaa ggatagggct accggcaata cgccagtagc tctttctcgt gttggcgtgt
    14581 tcccaagcct tgtaacctgg tgcacctagt gcgacaaggt tgttgtgctt ggttttgatt
    14641 ttcttccatt gcttccaaaa acacattcgt acccttctgc gaagccacat atccaatgat
    14701 ttagcgagtc ctctcatgtc tgcaatgcca aagtaattga cccatccgac aataatttgt
    14761 cttaacttaa ccatcctctg gtacatactc atggcattac ttctacccgt gatttccttt
    14821 agtctttgct taagtttagc aacaggttta ggatgaactc gtatcccgat ttcgcccttt
    14881 ttgcggtaaa atgagaaccc aagaaactta agcttccacg gtctatctac agagcttttt
    14941 gctttgttga ctttgagctt tagctcttct tcaataaagc gagtaatgct tgccatgact
    15001 ctgtctgccg cttttctgct tctgacataa atattgcagt catcggcata acggcaaaat
    15061 ttcaagcccc tttttgtcag ctctttgtcc aattcgtgaa gcataacgtt actcaagagc
    15121 ggagacaagt ttccgccctg cggtgttcct tcctccgtcc ccattacaac cccattaatc
    15181 ataactccgg actgaaggta cttacgtatt aatgacaata cccccgaatc cttgatggtt
    15241 tctgatagta atctcatgag cttgtcgtgg ttgacagtat cgaagtattt agcaaggtca
    15301 atatccactg accaattgta gccagcttca atgtattcct tgcatttcat gaccgcttgt
    15361 tttgcactcc gtccaggacg gaatccataa ctattatcca aaaatcgctt ctcgtagatt
    15421 ggactaagaa cttgggctat ggcttgctgt attactctat cgagcactgt aggaatccca
    15481 agcagtcttt tacccccgtc aggtttgggt atctctaccc gcctaacagg gctggggcgg
    15541 tattccccgt tccgaatgga ttgaatgatt tggttgccat tttgttttag gtattgtaga
    15601 agttcatcca ctgtcatacc atcaactcca tggctgcctt tgtttgcctt aacccttttg
    15661 taagcccggt tcatgttgtg gcggtcaagg atcttctcaa gcaatcctcc ggcatatcca
    15721 ttgccaccgt ttccatctac ttcagacgtg gaagaaatat tccgcgctcc tgcattacct
    15781 ccgagttcca cccgtacttc ctgcaggtag ccttcttttg gaagttgtct gctttctaca
    15841 tatttcttcg tgtctctcaa tttcaggaaa cctcctattg ttcagtcctt tctcctccgt
    15901 acatgtccag aagagtacta tgacctatgc tgacttctgg cagttcagcc atacatcact
    15961 gcatgggttg ccgtttctga atacacatcc acagcgtacc tgccagacct ccccgggtaa
    16021 gaacgatagc cttcatccca tctacccgcc gcatctactg tatggagttt gtgcagtgtt
    16081 ggacttcgtt ttgtatcgca aactcgtcca ctccaattca gccttatatg cggttcttgt
    16141 tcatcgggcc gggactttgc ctctggcttc cttcagattc cacctcacga tggacaccct
    16201 tgcctttggc taatggttcc cactgcaaag cccatagcgg actttcaccg ccaagctacc
    16261 gcccatgccg ggcacacgat aaagacggcc gtccatccgt ctgcccgcgc tgcagtaacg
    16321 aagagctaaa tggggaatac tgcaagatct gcggtacata tatcgtcaat aaatgttcag
    16381 cagaaacaga tctcgacttg aacgggaacg aatatg
//
LOCUS       DASRMS010000039        37086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_746977, whole genome shotgun sequence.
ACCESSION   DASRMS010000039 DASRMS010000000
VERSION     DASRMS010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 37086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37086
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_746977"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFC74_01700"
     CDS             <1..244
                     /locus_tag="VFC74_01700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24081.1"
                     /translation="YYLVVGSVEPHHVLVVNGLNRPLKSPKKKNLRHLQIQRIAPTEL
                     VDRIKQRKVRDEEIARAIRGMVCEDQPGGEREGNIT"
     gene            244..462
                     /gene="infA"
                     /locus_tag="VFC74_01705"
     CDS             244..462
                     /gene="infA"
                     /locus_tag="VFC74_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HZK24082.1"
                     /translation="MSKKDAIEVEGTVIEPLPNAMFRVELKNGHKVLAHVSGKIRMNF
                     IRILPGDRVLVELSPYDLTRGRITYRYK"
     gene            485..598
                     /gene="rpmJ"
                     /locus_tag="VFC74_01710"
     CDS             485..598
                     /gene="rpmJ"
                     /locus_tag="VFC74_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HZK24083.1"
                     /translation="MKVRPSVKPICEKCKIIKRKGKVMVICENPKHKQKQG"
     gene            669..1040
                     /gene="rpsM"
                     /locus_tag="VFC74_01715"
     CDS             669..1040
                     /gene="rpsM"
                     /locus_tag="VFC74_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HZK24084.1"
                     /translation="MARIAGVDLPREKRVEIALTYIYGIGRKTAQDILNQSGINMDTR
                     VRDLTEDEVNRLREYIDKNNKVEGDLRREVAMNIKRLMEIGSYRGIRHRRGLPVRGQT
                     TKNNARTRKGPKRTVGAKRKK"
     gene            1061..1456
                     /gene="rpsK"
                     /locus_tag="VFC74_01720"
     CDS             1061..1456
                     /gene="rpsK"
                     /locus_tag="VFC74_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HZK24085.1"
                     /translation="MVARKRTARPRRRERKNIQHGVAHILSTFNNTHITITDLKGNTI
                     SWASSGGVGFKGSRKSTPFAAQSAAEKAAKAAMEHGLKEVEVFVKGPGSGREAAIRSL
                     QAAGLEVNLIKDVTPIPHNGCRPPKRRRV"
     gene            1475..2104
                     /gene="rpsD"
                     /locus_tag="VFC74_01725"
     CDS             1475..2104
                     /gene="rpsD"
                     /locus_tag="VFC74_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HZK24086.1"
                     /translation="MARYTGAVCRQCRREGLKLYLKGERCYTEKCGVDRRAYAPGQHG
                     QRRRGKTSEYGLQLREKQKARRIYGVLESQFRRYFREADRRKGITGENLLQILEARLD
                     NIVYRMGFALSRPEARQLVKHGHFSVNGKKVTIPSYQVRPGDEIAVREASRTKGRFKE
                     LAELTQNQGTMEWLEVNRDNLAGKVVRLPNRDEIDIPITEHLIVELYSR"
     gene            2167..3114
                     /locus_tag="VFC74_01730"
     CDS             2167..3114
                     /locus_tag="VFC74_01730"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HZK24087.1"
                     /translation="MIEIEKPKIERVDSNDDIAYGKFVIEPLERGYGITLGNSLRRVL
                     LSSLPGAAITSVKVEGVEHEFSTIAGVLEDTTEIILNLKKLAMKIHADEPQTLTIDAS
                     VQGAVTAGDIKLPADVEILNPDLHIATLEDGGNLYMELFAANGRGYVQAERNKEAGQP
                     IGIIPMDSLFSPIHRVNYQVEDTRVGQHTDYDKLTLEVWTDGSIHPDEAISLGAKVLS
                     KHLGLFINLTEKVAEVEILDEKEEDDKTKVMEMTIEELDLSVRSYNCLKRAGINSVQE
                     LVQKSEEEMMKVRNLGKKSLEEVTQKLDELNLSLRDSNE"
     gene            3130..3468
                     /gene="rplQ"
                     /locus_tag="VFC74_01735"
     CDS             3130..3468
                     /gene="rplQ"
                     /locus_tag="VFC74_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011459109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HZK24088.1"
                     /translation="MVLRKFGRRSEHRRAMLRNLVTSMILADKVMTTEAKAKQIRSIS
                     EKMITLGKRNDLHARRQALAYLLDETAVKKLFEEIAPRYEERQGGYTRILKMGPRRGD
                     AAPMAIIEFV"
     gene            3581..4411
                     /locus_tag="VFC74_01740"
     CDS             3581..4411
                     /locus_tag="VFC74_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018596679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZK24089.1"
                     /translation="MEIIRAAGLSYKYHKEDGGEFWALRDIHLGINEGEFVAILGPNG
                     SGKSTLAKHFNALLLPTNGEMYVGGLNTREIENHWQIRQTAGMVFQNPDNQIVATTIE
                     EDVAFGPENLGLPSTEIRQRVDEALELVGMSAFKQQAPHLLSGGQKQRVAIAGVIAMR
                     PRCLILDESTAMLDPRGRCEVLETVSRLNKEEGITVILITHFMEEAVAADRIFILEKG
                     HIAMQGQPQDVFLRVDDLRTLGLDVPPVAELAGELRQCGVELPPSVLTVDELVKQLCC
                     "
     gene            4402..5253
                     /locus_tag="VFC74_01745"
     CDS             4402..5253
                     /locus_tag="VFC74_01745"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010295168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HZK24090.1"
                     /translation="MLLTVENLSYTYMPGAPFEVKALQNINLNIAQGEYLAIIGQTGS
                     GKSTLVQHLNGLLKPTSGRILLEGKDIRNAKSGLKTLRRRVGLLFQFPEQQLFEETVF
                     ADVAFGPKNMGLDEAEIKQRVEKALILVGLPLDVAERSPFGLSGGQMRRVAMAGVLAM
                     QPDVLILDEPAAGLDPRGKAEIFDQIDRLHQEENLTVILVSHSMEDVAVRAKHLLVLA
                     DGQVKLHGTPAEVFTDAAELEQLGLALPQMALLMHRLRQAGKEVPTDIFTVSAARDAI
                     LQMLEVQ"
     gene            5258..6052
                     /locus_tag="VFC74_01750"
     CDS             5258..6052
                     /locus_tag="VFC74_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012344030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZK24091.1"
                     /translation="MKSITIGQFIPGESLLHKLDARAKFVALIFLIIAIFSVHSFWGY
                     LILAGVIFSLLLISRIPLSYFFRGLKPVYYIMIFTLVIHFFFTKGGTVLLRLGSVTIE
                     TGGIYFGFFMVTRIIFLVVATSLLTLTTSPIALTDGLEYLMKPLSKVGVPAHEIAMMM
                     TIALRFIPTLMEESEKIMKAQMARGADFESGNIYQRAKSLVPLLVPLFVSSFRRADDL
                     AIAMEARCYRGGEGRTRLRELQATTKDYLVIAASITLVILITWGQV"
     gene            6112..6843
                     /gene="truA"
                     /locus_tag="VFC74_01755"
     CDS             6112..6843
                     /gene="truA"
                     /locus_tag="VFC74_01755"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HZK24092.1"
                     /translation="MKNIKLTLSYDGTNYHGFQLQQNADTVQAQLEAALQTVFGEAIR
                     ITAAGRTDTGVHALGQVVNFQVNTRIPLARFPYAINANLPADIVVTEAAVVPDHFNAR
                     FSSQSKVYRYTIDCAPFPRVLTRHYTYHLRYPLDVQLIQRAATTLVGKHDFYSFMASG
                     SSVKTTERHIMRLDVEEQEQYLIITAEADGFLYNMVRIIVGTLIEVGRGKRSPDLSAV
                     ITARNRAAAGWTAPAYGLVMREVKY"
     gene            6987..7418
                     /gene="rplM"
                     /locus_tag="VFC74_01760"
     CDS             6987..7418
                     /gene="rplM"
                     /locus_tag="VFC74_01760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HZK24093.1"
                     /translation="MRTTFMPKEGQIERKWYILDAADKPLGRLATEVARILRGKHKPI
                     YTPHVDTGDHVIVINAGQVVLTGNKATQKFAYRHSGYPGGLKKVSYGTLIANKPERAV
                     MLAVKGMLPHNRLGRAMLKKVRVYAGSEHAHEAQQPEVWTF"
     gene            7441..7833
                     /gene="rpsI"
                     /locus_tag="VFC74_01765"
     CDS             7441..7833
                     /gene="rpsI"
                     /locus_tag="VFC74_01765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HZK24094.1"
                     /translation="MAQVQFYGTGRRKNAIARVRLVPGSGEITINSRDIDQYFGLETL
                     KLIVRQPLKLTDTLEKFNVIAKVEGGGTTGQAGAIRLGIARALLKAEGNLRPALKSAG
                     FLTRDPRMIERKKYGLKKARRAPQFSKR"
     gene            7949..8644
                     /locus_tag="VFC74_01770"
     CDS             7949..8644
                     /locus_tag="VFC74_01770"
                     /inference="COORDINATES: protein motif:HMM:NF015549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HZK24095.1"
                     /translation="MTTKQQFMAGAGKAIPVVLGYLPLGFAYGALASLAGISLPHIIL
                     MSLLVFAGSGQFIAVNMFALGAGSLSIIVTVFLVNLRHLLMSASLSPYLQKFPRQSLP
                     LLAFGLTDENYALACGELSKQERNHNFLLGLFATSHAGWVCGGALGGIFGQLFSSARA
                     AYTMEYALYAMFIFLLVIQVTDKKLLLAASISGVLSLLFYQLIPGSWYIILATVATAT
                     LGVMIEKWQGSLS"
     gene            8623..8940
                     /locus_tag="VFC74_01775"
     CDS             8623..8940
                     /locus_tag="VFC74_01775"
                     /inference="COORDINATES: protein motif:HMM:NF017273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HZK24096.1"
                     /translation="MARVFILILGMAIVTYLPRFLPMYILTRLEIPQVVSTWLRYIPV
                     AVLAALIVPGILAVNGHITVSPNNYYLLASFLAFGVAYRFRNMMLTVTVGMAAVLILQ
                     NLW"
     gene            complement(9090..9836)
                     /locus_tag="VFC74_01780"
     CDS             complement(9090..9836)
                     /locus_tag="VFC74_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HZK24097.1"
                     /translation="MQENELITAQELAAILCLSVDTIWRYTREKKIPFVEIGNRQYRY
                     HKQSVLRALAGKAAVAEDSSRYEGKKKLTYEDYASLPEDPGFRYEVIDGVLLRDPSPS
                     FQHQRVSRRLQRILEDYFAVVDPKGEVFNAPLDLTLAEYNVVQPDLLYLPGSSPAQAT
                     PIKTVPELVVEVLSPSTSRKDRVLKLNHYQAAGVLHYWILDPENCFIEAYELRDEHYV
                     SMVRAGKGTFTHPSFPGLTFEFEALFGQPS"
     gene            10123..11202
                     /locus_tag="VFC74_01785"
     CDS             10123..11202
                     /locus_tag="VFC74_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24098.1"
                     /translation="MSKTYWGDITAIGSLPDIGTAAGLELVLHSLKHIPHWPQFPKRS
                     QEYLTHQFLQVLVDCGLMEVPEQGNPVLNEQTPTFLEGLTAFYQTYLEAESGDLEALA
                     SFAIPDWAGEGFAALIKELENNPALQPQYLKGQLIGPLTAGLQIMNADNIPIIYDDQL
                     RDVVIKALTMNIRWQVSQLSRTGLPVIIFLDEGLLHAYGHRDFLGLSAQLISDTFTEL
                     ITAIKDSGAEAGIHFCSRADWELLFPAQPDILAFDAYSYFSGMLAAAEQVNTFLAQGG
                     TIAWGLIPSYEACFTETAASLSQRWRDYITRLEQKGVDGARLRKQYLITPSCGTGFYT
                     PAIAQRVYQLLQETGDLIRQTCFDF"
     gene            11738..12574
                     /locus_tag="VFC74_01790"
     CDS             11738..12574
                     /locus_tag="VFC74_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK24099.1"
                     /translation="MCEFCTQHGEGKKWYEVMQNYSGELLANEGRMQYIRHFIPNVQK
                     NAQANIAKLAWAKQHFPAVYRFIRSIGTKRLKQIHFGQVVPLEDAEKVIDLVQSITRT
                     ACVCRSVVSGKKNARYCLLLGVDPYQSGIDYADLQHSLETITPTEAKQLLREFDQQGL
                     VHSIWTMKTPFIGALCNCNQECLAYQVQVESKLLEMMFKAEYIAEIEAALCTGCRRCQ
                     QLCQFEAIAYEHGQCSINTQKCYGCGVCRQACPAEAIVLRPKKIIASGCPEIVETERA
                     HR"
     gene            12605..13123
                     /locus_tag="VFC74_01795"
     CDS             12605..13123
                     /locus_tag="VFC74_01795"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HZK24100.1"
                     /translation="MDNLPEYLDDNLKVVFVGYNPGEKAAALGHHYAGRGNQFWRLLF
                     DAGLTNRLYLPTEDGKLLQLGYGLTNIVSRPSRSSSELHKKELQAGAEVLREKLRRFQ
                     PYLVCLLGKEVYRHYAGLKSSASYTFGAKATATIPGIKEFAAPNPSARSTIPYGVKLA
                     YFVQISQLLRSR"
     gene            complement(13290..13412)
                     /locus_tag="VFC74_01800"
     CDS             complement(13290..13412)
                     /locus_tag="VFC74_01800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24101.1"
                     /translation="MKLDYKRTILVGLAFLTISSFWQMYDFYIPLILNSDYCAA"
     gene            complement(13529..14572)
                     /gene="rsgA"
                     /locus_tag="VFC74_01805"
     CDS             complement(13529..14572)
                     /gene="rsgA"
                     /locus_tag="VFC74_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZK24102.1"
                     /translation="MLIEDYGWDAYFAEHWHSQAKDEMFPGRVVADYGQKLRLISEIG
                     ELEVARPLTKENQAVSIAVGDWVALEQLSEDTVGVRFVLPRKTKFSRAAAGNAVKEQI
                     VAANADTVFLMQSLNRDFNMKRLERYLIAAWESGAAPVVVLTKADCCTDVAVKVAKAQ
                     DTALGVAVYAISCLTKAGLKDLQRYFAPGKTVTLLGSSGVGKSTLVNLLLNKEQFSTQ
                     TIRENDSRGRHTTTHRELVLLPSGGLIMDTPGMRALSLWEAEQGMEIMFGDVEELIQH
                     CRFSDCQHQTEPGCAIRAALEAETLMVPRWESWLKLQKEKTHLEAKREGTLRLQEKQW
                     GRQFAKLHKSKRK"
     gene            15552..16151
                     /locus_tag="VFC74_01810"
     CDS             15552..16151
                     /locus_tag="VFC74_01810"
                     /inference="COORDINATES: protein motif:HMM:NF025890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZK24103.1"
                     /translation="MKKKQVLSLTLLTLVLILAMILLAGCGGGEETEAPPINEGDTPP
                     VDHVVTMPDDYESTDYCLECHIIEPYVESWESSSMLANSHQQAGINCVNCHERTEEIT
                     AQEIEKYNNGDYMDPLVSTQMPMEDCLQCHESYEALAERTADYTYEGEQVNPHDSHLG
                     EQECSSCHTMHQESSGINYCLSCHHEGGLVSCYECHDHI"
     gene            16208..17977
                     /locus_tag="VFC74_01815"
     CDS             16208..17977
                     /locus_tag="VFC74_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK24104.1"
                     /translation="MSKETKETKGTKRSRRQFIKDAALVAGATAVGGFFLTGCSDGAA
                     EGGEEITWDKETDVLVVGTGCGLAGAIEAKNAGADVLVLERNDWAGGLYITTGGHAIL
                     GGTHIQKEEGIEDSIEAWYEDEIKSAGHRGVPELIRTYTERGPEFVEWMESMGFKWQK
                     PLGHSGAEVHRVPRSHYAGSNPSVYQSQTGAARSFGLAWTTVWMKKIEELGIPILYKH
                     RMRKIYRENGGPVIGVSCDTPDGTVNIKARRGVVLTTGTWTDNYRMVQAWDPRAVGPD
                     CYGDGGTPCDGTLFVDSSGDGHLAAAEVGAGFSDMSFVSYYYLFYGSRSYWGWEPPDF
                     KTADYYMAGKGLTRNKAFFERVVLVNNDGKRYINEMEGTRSSDPELGEAGGLNENPEW
                     PYTAKYLALPQPRNVWAVTDAEGAAAMKWPIEDLENPNPKKGAMFDPACIAIADSIEE
                     LASKMGVDVAGLEETLTRYNGFADAGEDEDFGKPMPLSKIATPPFYAAKASLIRHTQR
                     NGIRVNTKAQVIEQADQVAGYDGTASGKSISIDDEKVIPRLYASGELANIMGWRRAHG
                     SMGNYAIFARIAGEQAAKEEPLE"
     gene            18115..19077
                     /locus_tag="VFC74_01820"
     CDS             18115..19077
                     /locus_tag="VFC74_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24105.1"
                     /translation="MTENAKVVAEKDPVHPHHIAVEMAKVPPANVYAGVFMTLKVKVS
                     CTAACSLKGQLVLILDHTDQVVKELRLITPYRGAYETDQFVLQAPHNPGEYTWTAVFP
                     QQERDNVSHKRAAADFNFTVKAHHATSIAVWDVTSPVAIKTASRVKVGVKCSVGCQLT
                     GRQIEIYNQSGHKAAEATLGDVPWKGTDSMYWTEITIPAPDTTGYSTWQARFSESELE
                     THLAKQASFGFTVVNSPECSMTVQVVERESKVPVSNASIIMHPYRIKTDEKGRATLAV
                     AKGEYKLFVTREDKYELWQQTVQVTAEQTITAELAVTPPIVHEG"
     gene            19120..19371
                     /locus_tag="VFC74_01825"
     CDS             19120..19371
                     /locus_tag="VFC74_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24106.1"
                     /translation="MFGGKLGTQELILILGVALLVFGPQKLPEIGKAVGKGLREFRKA
                     TTELKNSIDSDAEQPAPAPKQEVPAETVAQTPNDVVNKE"
     gene            19386..19532
                     /locus_tag="VFC74_01830"
     CDS             19386..19532
                     /locus_tag="VFC74_01830"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24107.1"
                     /translation="MKIGWLELGFILLIVLVVMGPGKFTGIFKGIKKGVSNFRESASS
                     DDEQ"
     gene            19574..20305
                     /gene="tatC"
                     /locus_tag="VFC74_01835"
     CDS             19574..20305
                     /gene="tatC"
                     /locus_tag="VFC74_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZK24108.1"
                     /translation="MGTKKDVAEMTIWDHLGDLRKRLIIMVITVLVATVISFAFINDI
                     LQFLIKPAPGLQLIFTSPPEALTAQIRLAIQVALIGTLPLNLVQLLIFIMPALEKTEK
                     KALLPFFLAMNLLFFLGVTFCYYLVIPFAIKFFMGFGTESLTPLFSIGNYVSFITSLL
                     LSFGAIFEIPLVFWFLGLMGLISSDFLRKNRKFAVLVIVIVAAIMTPPDVVSQILMSI
                     PLLFLYEAGTLLVRLTERQKAKRAA"
     gene            20909..21163
                     /locus_tag="VFC74_01840"
     CDS             20909..21163
                     /locus_tag="VFC74_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24109.1"
                     /translation="MFGGRIGTQELILILGVALLVFGPQKLPEIGKAVGKGLREFKKA
                     TNEFRSSIDVDTEQPAAEPKQDVPAQPVVNATDEVAVSNE"
     gene            21178..21324
                     /locus_tag="VFC74_01845"
     CDS             21178..21324
                     /locus_tag="VFC74_01845"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24110.1"
                     /translation="MRIGWLELIVILFILLMVMGPGKFAGIFKGIKNGVANFKESVSD
                     EEEQ"
     gene            21414..22811
                     /locus_tag="VFC74_01850"
     CDS             21414..22811
                     /locus_tag="VFC74_01850"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK24111.1"
                     /translation="MKEENRKGTLVEDAVKGINRRNFLKGAAVSALSVTSLGMLAGCS
                     PLNKEDESANDAKKVVYELPDREGKLTLEELNALRQQYVDSKEEYVCEDGTVIPAVYV
                     KLRALINTYGNGAGSEVHDASFNEVMYLFSEKEAQAYLEMPFGVRFTATDFSVKSGRD
                     ETDCLKLCEDLAARGLLKRLRRGGVAYFHHLAPAHGIWEYVLLLPENQNKEYVTKHKS
                     MWGADIVSDLYDIDTPFYYPIPVDKEIVVEDEVLSYDDYEKIVARNEVIAVSTCQCRL
                     GQDIDGSRAPDCDHPMETCITTGEQAEFYIENGIGRQIDQEEALKILRHSVEVGMVLQ
                     SCHTSSTEVICSCHGDCCDVLKSYVALGENYDKLGISEVVSHYELQYNRDECVKCRLC
                     EPVCPMFAITMDDEGYSVTGSPCISCGQCATVCPAGARKLVQREGADQTKLPGSLLDD
                     YNLKASYRFKNNMIS"
     gene            22905..23303
                     /locus_tag="VFC74_01855"
     CDS             22905..23303
                     /locus_tag="VFC74_01855"
                     /inference="COORDINATES: protein motif:HMM:NF013334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HZK24112.1"
                     /translation="MHEMALTGDVVDIVVNKAEAVGATEVRAVYLTIGYVRDIVEDIF
                     ERCFAYLARNTVAENAELVITRVPFTVRCNNCEHVYHLDVHDELTWICPVCNAKDYKL
                     HTGMEFFVNNIEVVGPAAPEGQEDQACAQK"
     gene            23442..24125
                     /gene="hypB"
                     /locus_tag="VFC74_01860"
     CDS             23442..24125
                     /gene="hypB"
                     /locus_tag="VFC74_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HZK24113.1"
                     /translation="MNELRTVEIKQEILADNSREADAFRAARRAEGTFMIDIMGSPGA
                     GKTTLLLALIEQLRGESGAEIGVIEADLASDVDALKIKEAGVAAVELNTKNVCHVEMG
                     MVEKAFAAFAGKRFDYVFLENIGNLVCPAEFDTGCHSRIMLLSVPEGYDKVYKYPPMF
                     AVVDALVVTKSDYLPLNPDFDRQALREGARALNPKLEIFEVSARTGEGMAELAAWFQA
                     RREAALNLA"
     gene            24157..24411
                     /locus_tag="VFC74_01865"
     CDS             24157..24411
                     /locus_tag="VFC74_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24114.1"
                     /translation="MCTNRINTNQLEQMIDQIDDHIALEYRWVHKLAHAADDEGFAAA
                     SEKLHAAQLLLADVRAALDEAKEALESSASSASDTTARLV"
     gene            24643..24897
                     /gene="tatA"
                     /locus_tag="VFC74_01870"
     CDS             24643..24897
                     /gene="tatA"
                     /locus_tag="VFC74_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015394138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24115.1"
                     /translation="MFGGRLGTQELILILGVALLVFGPQKLPEIGKAVGKGLREFKKA
                     TTEFKNSIDIDAEQPAPAPKQEVPTETAAQTADETVVNHE"
     gene            24912..25058
                     /locus_tag="VFC74_01875"
     CDS             24912..25058
                     /locus_tag="VFC74_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HZK24116.1"
                     /translation="MRIGWLELGFILLIVLVVLGPGKFTGIFKGIKKGVSNFRESISR
                     DDEQ"
     gene            25100..25831
                     /gene="tatC"
                     /locus_tag="VFC74_01880"
     CDS             25100..25831
                     /gene="tatC"
                     /locus_tag="VFC74_01880"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HZK24117.1"
                     /translation="MGIKKDISEMTIWDHLGDLRKRLIIMVITVLVATVISFAFINDI
                     LEFLIKPAPGLQLIFTSPPEALTAQIRLAIQVALIATLPLNLIQLLIFIMPALENTEK
                     KALLPFFLAMNLLFFLGVAFCYYLVIPFAIKFFMGFGTESLTPLFSISNYVSFITSLL
                     LAFGAIFQIPLVFWFLGLIGLISSDFLRRNRKFAVLVIMLVSALMTPPDIISQVLMAI
                     PLAFLYEAGVLLVRLTERQKAKKAA"
     gene            complement(25903..26118)
                     /locus_tag="VFC74_01885"
     CDS             complement(25903..26118)
                     /locus_tag="VFC74_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK24118.1"
                     /translation="MEVKIKSRIKELRARYDLTQEQLAQLVNVRRETIGHIENNKYNL
                     SLILAYKISRALHSTIEEVFSFAELEQ"
     gene            complement(26120..26449)
                     /locus_tag="VFC74_01890"
     CDS             complement(26120..26449)
                     /locus_tag="VFC74_01890"
                     /inference="COORDINATES: protein motif:HMM:NF024088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3796 domain-containing protein"
                     /protein_id="HZK24119.1"
                     /translation="MKILKMFGWLGLLGLLGIFTDNVGFFGFFGFFGFWGMAKIKDDE
                     LLRKNLHKAGFNAFVVSLASLAAAIAGVAVVKTLEAAVMFIAVTFVVQIITFVISFNV
                     SEKRGES"
     gene            26772..28601
                     /gene="pepF"
                     /locus_tag="VFC74_01895"
     CDS             26772..28601
                     /gene="pepF"
                     /locus_tag="VFC74_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HZK24120.1"
                     /translation="MTKVQAVKRLPLRKEIAEKDTWRIEDIFPSDEAWEKEFQEVKAM
                     VPGIKDYQSRLDESAETLYAALQDQDQLTLRLGKLYAYAHLRYDQDTTNSIYQALNER
                     MRNLYAQAASQLAFIVPELLSIAEEKLQAFLEQKAELRLYEHALAEINRQRPHVLPAA
                     QEALLAEASEVLGASQNTFGMLNNADLEFPVIKDDKGEEVEVTHGRFIRFLESADQRV
                     RHDAFNAVYDTYGKFRNTFASTLSGNIKKNNFYARIRNYASAREAALAKNNIPESVYD
                     NLVSTVNEHLPLLHRYLKLRKQVLGLNKLHMYDLYTPLVKDVKMEIGYAQAQDLIMAG
                     LAPLGEDYLKVLQEGFANRWVDVQESKGKRSGAYSSGAYGTNPYILLNWQNNLTDLFT
                     LAHEFGHSVHSYYTRKYQPYPYGDYAIFVAEVASTCNEALLNDYLLHTITDQQKRLYL
                     LNHFLEGFRATVFRQTMFAEFEHLIHQQAQNNEALTAESLTKDYYQLNQKYYGTEDMI
                     IDEQIGLEWARIPHFYYNYYVYQYATGYSAATALSKGILTTGTPAVARYLEFLQAGSS
                     DYPLAVLQKAGVEMTSKKPIEAACKVFAEKLTEFAQLAGIQTS"
     gene            complement(28762..29211)
                     /locus_tag="VFC74_01900"
     CDS             complement(28762..29211)
                     /locus_tag="VFC74_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24121.1"
                     /translation="MSLKQLLLLTSCLLLFTLQRATGLATQVKNAQQEVTEARELTLA
                     SEKYETQLMNITKYAPLSAGAIPGHDYAGDPSVTASGEKLVPGKTAAAGENIPFGTEI
                     YVEGEGWYAVEDRGGNIGPNDIDLACKSKEESIEFGQKQLLVILKAP"
     gene            29476..30363
                     /locus_tag="VFC74_01905"
     CDS             29476..30363
                     /locus_tag="VFC74_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24122.1"
                     /translation="MNKRDKALFVCLIVVVLLNWWQINSSQKLMNGISQMQSEINSLR
                     FQISNEIGSVSGVVQQIQDESRWWALDETEILNAGREEAEVKISWYLREYQEDSLVTF
                     NYRPVGENDFTTVAAVKGSEGYFYSILNLAVIPEPMWDISISHSSNSNQSDTFEEVYD
                     KERVELSQKMRYEYYVATTKDGVIRTGEVMQLDLNEMRDYLFNSLHTHVSTQQNRPTY
                     ISLVETKYLENVPYRLVEAHVETRMGNRVIEKWPLEKQGDTNEAAVFESRIETTQDYD
                     DLYIVVRYSEGFTVERKIQ"
     gene            30576..30737
                     /locus_tag="VFC74_01910"
     CDS             30576..30737
                     /locus_tag="VFC74_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24123.1"
                     /translation="MQKYDTFAQFKEITQKNRAEKEKELGYTMLDTNKGRDVCIRQIE
                     RKIEQVIDE"
     gene            31165..32163
                     /locus_tag="VFC74_01915"
     CDS             31165..32163
                     /locus_tag="VFC74_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24124.1"
                     /translation="MKLAENYREQFLAWAKDNLLLERESSTEELYAALLKDKPVLAKR
                     INKAAPKTGAKAYIGRYLLRAFVNQGWLKYRDKKWFVQPLEDHCANCLTELHPDIYLV
                     DTVGSYYCCEDCLLEREDGATDGYWMDYLPLFADFADLLPESSDHKQQKKEADPLTHL
                     EVINIRKRLANVINCPDYDTIWLNGGDDGPLAAEMYRMLTMLAARLTKVTEVETAIAK
                     LRAPQQVNYTIQLPEGLPNEKRLQVKVERFSKRHKQIRRQDLPLAWRTDDLSLLSRWE
                     KQLKEISYTIVKSRKCPLCSIDADNFALAVDGYRYCEACYEYYELQSYQVEEEYDW"
     gene            32662..33009
                     /locus_tag="VFC74_01920"
     CDS             32662..33009
                     /locus_tag="VFC74_01920"
                     /inference="COORDINATES: protein motif:HMM:NF040311.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6054 family protein"
                     /protein_id="HZK24125.1"
                     /translation="MAQTFSVEVEAEASELYAYLCHKMSHVQKVSEDVFSDTEQYVAV
                     LIYEQYFWRVGNQIALTVIINRLESGRCCLKTISCGSSKGMVFKLDLGAASAFAREPI
                     NYAEERYNVIWQR"
     gene            33106..33927
                     /locus_tag="VFC74_01925"
     CDS             33106..33927
                     /locus_tag="VFC74_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HZK24126.1"
                     /translation="MNFTIGVCQLNVADNKELNLKNAERAIREAAGKGSQLVVLPEMF
                     NCPYENKAFPLYAEEYPGETTSMLGKLAKELKIYLVGGSIPERDHDAIYNTSFIYDKN
                     GALIGKHRKMHLFDVDVEDGISFRESSSLGSGEQVTVVATEFCRIGVAICYDMRFPEL
                     MRLMALAGAEVIIVPAAFNLTTGPVHWEITAKARALDNQVYFVAASPARNLQAVYHAY
                     GHSCIIDPWGQVVNELDEKEGILYGEIDLQFVQKVRRELPLLQHIRKDIYEIRKK"
     gene            complement(33943..35232)
                     /locus_tag="VFC74_01930"
     CDS             complement(33943..35232)
                     /locus_tag="VFC74_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009615550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HZK24127.1"
                     /translation="MNISLPGDVAYILNKLHEHGFAGYIVGGCVRDSLLNKVPHDWDI
                     TTNAQPDQVKQLFAKTIATGIKHGTVTAVINGKQYDITTYRHAGNGTGVRRSDNTTSL
                     KEDLSRRDFTINAMAYQQQTGLVDYFNGVSDLRCAVINAVGDPEQRFQEDALRMMRAI
                     RLACQLGFTVGTSTLTAITRNHQLLRHISAERIRNELCRIMLSKQPARGIELLHSTLL
                     LSMILPELTACADFDHVLAVLDAAPKGLTVRLAALLHGIGKPISKKENKSGDLSRYHL
                     ERADIVRDILRRLKFAKQTVDSVCILVREQLNRFDFPNKANIKRLINRVGLGNLQSFF
                     ELQIAEIKASQPPHHFSALNALSEQVCKIIKEKQPLTVKDLAVNGDDLITIGIQPGVQ
                     MGQILNSLLEKVLEEPEMNTKENLLNIITTEQGENNI"
     gene            35488..36726
                     /locus_tag="VFC74_01935"
     CDS             35488..36726
                     /locus_tag="VFC74_01935"
                     /inference="COORDINATES: protein motif:HMM:NF027437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4829 domain-containing protein"
                     /protein_id="HZK24128.1"
                     /translation="MKKFFLILFSLLLVLLLTGCSPNQNRSEPKGEAMFQPSVAGLKA
                     ITLDTIEVTDPSLISLLSAELAKAVPSEQAPDNAHPETLTLTVNGREESYQIYIPGLD
                     TAAPAVLKSAEGWYQIPAEFINLLYSLTEYPNYSAAVDAKDKELLAEYGLTPAFLINQ
                     LQVKLPTDLLHRAGEYPIILYWAHNNELSQAINLNLEKALGQQVDVRLYKIVELLPDY
                     LSPRCHTGRAVIVHHADKIIGAWLDAGRHYGFACSLDGKTLEEVTNKSWDQWIVSHID
                     YDDPLEKELALKSPEDIIQTYYAAINNKDDKRAYACLSRENLSSYLFSNLDNYYLMNP
                     EYEAYDGFANITSASVIKIEPYSLNSEQSEELLFYQVTVDLEVKEPMTFESGSQTRFI
                     HLKQESPQTGWRISSIGTGP"
BASE COUNT        10593 a         7890 c         8640 g         9963 t
ORIGIN      
        1 gtattacctg gtcgtcggca gcgtagaacc gcatcatgtg ctcgttgtca atggcctaaa
       61 ccgcccccta aaaagcccaa agaaaaaaaa tcttcgtcat ttgcagatcc agcgaattgc
      121 acccactgaa ttagtggaca gaataaagca gcgcaaagtg cgtgatgaag aaattgcacg
      181 ggcgatccgt ggtatggttt gtgaagacca gcctggggga gaaagggagg gaaacattac
      241 ctgatgtcaa aaaaagatgc tattgaggta gagggaacag taatagaacc actaccgaac
      301 gccatgttcc gagtggagct aaaaaatgga cataaggtgc tggcccatgt gtcgggaaaa
      361 atccgtatga actttatccg tatcctccca ggggacagag ttttggtgga attatcccca
      421 tatgatttaa cacgtggtcg gattacctac cgctacaagt aagacgccaa aaggaggttt
      481 tgggatgaaa gtaagaccat ctgtgaaacc gatttgcgag aagtgcaaaa tcattaagcg
      541 caaaggcaaa gtcatggtga tctgtgaaaa cccgaagcat aagcaaaaac agggttaatt
      601 gtataaaaag ggaaaagtct ggaaattata attctgtttg ttgaaaaaga aagggaggtg
      661 caaatcctat ggcgaggatt gccggagtcg acctgccgcg tgaaaaacgc gtggaaatcg
      721 cgcttaccta tatctatggc attggccgta aaacagctca agatatccta aaccagtcag
      781 gcattaacat ggatacccgt gttagagacc tgacagaaga tgaagtcaat aggctacgtg
      841 agtatatcga taagaacaac aaagtagaag gcgacttgcg tcgtgaagtt gccatgaata
      901 tcaagcgttt gatggaaatt ggcagttatc gcggaatccg tcatcgcaga ggattaccgg
      961 tacgtggtca aaccactaag aacaacgccc gtacacgtaa aggtcccaag cgcacagtcg
     1021 gtgctaaacg caaaaagtaa gtagataggg gggaaatgtc ttggtagcac gtaaaagaac
     1081 agcacgtccc agaaggcgtg agcgtaaaaa tattcagcat ggcgtcgccc atatcctgtc
     1141 cacttttaac aacacccaca ttacgattac tgatttaaaa ggtaatacta tttcttgggc
     1201 cagctccggt ggtgttggct ttaaaggatc tcgtaaatca acaccctttg ctgcccagtc
     1261 tgctgcagaa aaagccgcta aagcagcgat ggagcatggt ttaaaagaag tggaagtgtt
     1321 tgttaaagga ccaggttcag gacgagaggc tgccattcgt tctttacagg cagcaggtct
     1381 ggaagtgaat ttaattaaag atgtaacacc tattccacat aatggatgcc gtccgccaaa
     1441 acggcgcaga gtttaacctg aggaggtcag tctaatggct agatatactg gagccgtctg
     1501 caggcagtgc cgccgtgaag gtttaaaact ttaccttaaa ggcgaacgct gctatacaga
     1561 aaaatgtggt gtagatcgca gggcgtatgc gcctggtcaa cacggccaac gccgcagagg
     1621 aaaaacgtcc gaatatggtc tacagctacg tgaaaaacaa aaagcacgcc gtatttatgg
     1681 cgtactagag agtcagttcc gtcgctattt tcgagaggca gatcgtcgta aaggtattac
     1741 cggcgaaaac cttttgcaga tattggaagc aagattagat aatattgttt accgcatggg
     1801 atttgctctt tcccgtccgg aggcgcgtca actggtgaaa cacggtcatt tctccgttaa
     1861 cggcaaaaaa gttacaatcc cctcgtacca ggtacgtcct ggcgatgaga ttgcagttcg
     1921 tgaagccagt cgcactaagg gtcgtttcaa agaacttgcg gaattaacgc aaaaccaagg
     1981 gacgatggaa tggttagaag tgaatcgcga caacctagcc ggtaaagtag tacggttacc
     2041 aaatcgcgac gagattgata ttcccattac cgagcatctc atcgtcgagc tctattcccg
     2101 ctaaactttg actaaaacga attgccctcg cgttggtaac ggaatcgaga aggagggtct
     2161 ggaaagttga ttgaaatcga aaagccaaaa attgaacgtg tcgacagcaa tgatgacatt
     2221 gcctatggaa aatttgtgat tgagcccctg gagcgaggtt atggcattac cttaggtaac
     2281 tctctccgcc gtgtgctctt atcttcgctg ccgggtgctg ccattacctc agttaaagtt
     2341 gaaggtgtgg agcatgagtt ttccaccatt gctggtgttt tagaggacac cactgaaatc
     2401 atcctcaatc tgaaaaaatt ggcgatgaag attcatgcgg atgaaccaca aacactgaca
     2461 attgatgctt cagtgcaagg agctgtcacc gctggcgaca ttaaactacc ggctgatgta
     2521 gaaatattaa atccggattt acacattgct acactggagg atggcggcaa tctctatatg
     2581 gagttgtttg ctgccaatgg tcgcggctat gtgcaagcgg agcgtaataa ggaggcaggt
     2641 cagcccatcg ggattattcc catggactca ctcttctccc ctattcaccg tgttaactac
     2701 caggtggaag atacgcgtgt tggtcagcat acagactatg acaaactaac tcttgaagtc
     2761 tggactgacg gcagcattca tccggatgaa gctattagcc ttggtgcaaa agtcctctcc
     2821 aaacatttag ggctcttcat caacctcacc gagaaggtgg cggaagtgga gattttggat
     2881 gaaaaggaag aggacgataa aaccaaagtt atggaaatga cgatcgaaga actggatctt
     2941 tcagtgcgct cttataactg cctcaaacgt gcaggcatca actctgtaca ggaactagtg
     3001 caaaagagcg aagaggaaat gatgaaagtt cgcaatctgg gcaaaaaatc actagaagaa
     3061 gttacccaga aactagatga attaaatcta tctctgcgcg atagcaatga gtaaaaggag
     3121 ggagaagcca tggtactacg taaattcggc cgtcgcagtg agcatcgccg ggcaatgttg
     3181 cgtaatttag ttacctccat gattcttgcc gataaggtta tgaccacaga agcgaaagcc
     3241 aagcaaatcc gcagtatttc tgaaaaaatg attaccctag gcaagcgtaa tgacttacat
     3301 gctcgccgcc aagctctggc ctatctcctc gatgaaaccg cggtgaagaa gttatttgaa
     3361 gaaatcgccc cgcgttatga ggagcgtcag ggtggatata cccgtatcct aaaaatgggg
     3421 ccacgccgcg gtgatgcggc acccatggcc ataattgaat ttgtgtagaa tatagagcgt
     3481 caagaaaatc gacccacaag ctgtcgattt tctttgtaat ggctgttttt tatttacttg
     3541 ccatgaacgc tctatatcat ctgcaccggg ggtgagtcca atcgaaatca ttcgtgccgc
     3601 agggctcagt tataaatacc ataaagaaga tggcggtgaa ttctgggcac tgcgtgacat
     3661 tcatttaggc attaatgaag gggaatttgt agccattctc gggcctaatg gttcaggtaa
     3721 atctactctt gctaaacatt ttaatgctct tttattgcct acaaatgggg aaatgtatgt
     3781 aggagggctt aatacacgcg agattgagaa ccattggcag attcgccaaa cggccggtat
     3841 ggttttccag aaccctgata atcaaattgt cgcgacgaca attgaagaag atgtggcgtt
     3901 tggcccggag aatcttggtt taccatctac tgaaattcga cagcgggtcg atgaagcgct
     3961 ggagttagtg ggtatgtctg cgtttaaaca acaggcgccg catctgcttt ccgggggaca
     4021 gaaacagcgg gtagcaatag ctggtgtcat tgcgatgcga ccccgctgcc tgattctaga
     4081 tgaatcgaca gcgatgttgg atccccgcgg tcgctgcgag gtgctggaaa ctgtcagtcg
     4141 tttgaataaa gaagaaggta tcaccgtcat actaatcacg cactttatgg aagaggcagt
     4201 agccgctgac cgcattttta ttttggagaa ggggcacatc gccatgcaag gccagccaca
     4261 ggatgttttt ttacgtgtcg atgacttgcg aacattaggt ttagatgtgc cgccggtggc
     4321 ggaactagca ggagagttac ggcaatgtgg agtagagctg ccgccttcag tgctgacggt
     4381 agatgaattg gtaaaacaat tatgctgcta acagttgaaa acttaagcta tacctatatg
     4441 cccggagctc cttttgaagt aaaggcacta caaaatatta accttaacat tgctcaaggc
     4501 gagtatctag cgattattgg ccagaccggt tctggcaaat caacacttgt acagcattta
     4561 aatgggctcc tcaagccaac cagcggccgc attttgctgg agggcaagga cattcgcaat
     4621 gctaaatccg gtcttaaaac tttaagaaga cgtgtcggtc tgcttttcca gtttcccgaa
     4681 caacaactgt ttgaggaaac agtattcgcc gatgtcgcct ttggccctaa aaacatgggt
     4741 ttagacgaag ctgaaattaa gcagcgcgta gaaaaagcat taattttagt cggtctccct
     4801 ctcgatgttg ctgaacgttc gccctttggc ctttccggcg gacaaatgcg gcgcgtcgcc
     4861 atggctgggg ttttagccat gcagccagat gtgttaatac tagatgagcc ggcagctggc
     4921 ttggacccac gcggtaaagc agaaatattc gaccagatag atcgtctgca ccaggaagaa
     4981 aatctgactg tgatcttagt ctcacatagt atggaagatg tagccgtgcg tgctaaacat
     5041 ttactggtgc tagccgatgg tcaagtcaaa ctacacggca caccggcgga agtatttacc
     5101 gatgcggctg agctggagca gttaggttta gccctgccac agatggcact actaatgcat
     5161 cgtttgcgcc aagccggtaa agaggtgccc actgatatat ttacagtctc ggctgcgcgg
     5221 gatgctatcc tgcagatgct ggaggtgcag tagccagatg aaaagtatca ccataggaca
     5281 atttattccc ggagagtcac tgctccacaa attagatgca cgcgcaaaat ttgtagccct
     5341 gattttctta atcattgcta tcttttccgt ccactcattt tggggttatt taatactggc
     5401 tggggtgatc ttctctttac tgctgatctc gcggatacct ttgtcatatt tcttccgagg
     5461 cttgaagcct gtctactata ttatgatttt tacgctggtg attcattttt tcttcaccaa
     5521 aggtggtaca gtcttactgc gcctaggctc agtaacgatt gaaactggtg gtatctattt
     5581 tggctttttt atggtgacac gcattatctt tcttgtagtt gctacttctc tattaacgtt
     5641 aactacgtca cctattgccc taacagacgg gttagaatat ctcatgaaac ccctcagcaa
     5701 ggttggtgta cctgcccacg aaatcgccat gatgatgacc attgctctgc gctttattcc
     5761 gacgctgatg gaagaaagcg aaaagattat gaaagcgcaa atggcacgcg gtgccgattt
     5821 cgaaagcggc aatatttacc aacgagcaaa aagtttagta ccgcttttag tacccctatt
     5881 tgtttcatcc ttccgtcgtg ctgacgatct cgccattgcc atggaagcac gctgctaccg
     5941 tggtggggaa ggacgtactc gtttacgtga actacaagca acgacgaaag actatctagt
     6001 cattgctgcc agtatcactc ttgtcatcct cattacttgg ggtcaggttt aaccttagaa
     6061 acctaattta acggcttagc ctgcccgctt gtttaaaggg ggaatcttcc agtgaaaaac
     6121 attaaattaa cactttccta tgatggcacc aattatcatg gttttcagct tcagcaaaac
     6181 gccgacaccg tccaggccca actcgaggca gcgctgcaga ctgtctttgg tgaagcaatt
     6241 cgtattacag cagcaggacg cactgatact ggtgtacatg ctttgggaca agtggttaat
     6301 tttcaagtaa atactcgtat tccgctggcg cgtttcccct acgctatcaa tgctaatttg
     6361 cctgctgata ttgtggtgac tgaggcggca gttgttcccg accattttaa cgcccgtttt
     6421 agctcacaaa gcaaggtgta tcgctatacg attgattgtg caccctttcc gagagtttta
     6481 acgcgccact atacgtatca tctgcgctat ccacttgatg tgcaattaat acagcgcgct
     6541 gcgactactt tagtcgggaa acatgatttt tactctttta tggctagtgg aagttcggta
     6601 aaaactaccg aacgccatat aatgcgttta gacgtagagg agcaagagca gtatctgatc
     6661 attactgctg aagcagatgg ctttttgtac aacatggtac ggattattgt cggcacacta
     6721 attgaagtgg gccgcggcaa aagatcccca gacttgtctg cagtcatcac cgcacgtaac
     6781 cgggctgctg ctgggtggac agcaccagcg tatggtttag tgatgcgtga ggtaaaatat
     6841 tagcagattc cacattattt tcgccccgtc tatcttattc acttgacagg cagggaacca
     6901 tactataaaa tgattttgtg tcaattcgcc gttttctgcc ccgttagcgg tgatttgcag
     6961 aagtgttttg atcgaggagg aaaagtatgc gcaccacatt tatgcctaaa gaagggcaga
     7021 tcgaacggaa atggtatatt cttgatgccg ccgataagcc tctgggcaga ctagcgacag
     7081 aagttgcccg tattttgcgc ggcaaacata aacctattta tacaccccat gtggataccg
     7141 gtgaccatgt aatcgtgatt aacgcaggac aagtggtcct gaccggtaat aaagcaacac
     7201 agaaattcgc ttatcgccat tcaggttatc ccggcggttt aaaaaaggtt tcctatggca
     7261 ccctaattgc aaacaaaccg gaacgggctg tcatgttggc agtaaagggt atgttacccc
     7321 ataaccgtct tggtcgtgcc atgctcaaaa aagttcgtgt ttatgctggt agtgagcatg
     7381 cgcatgaggc acaacagcca gaagtttgga cattttaggg tagaagggag gaaactttaa
     7441 atggcacaag ttcaattcta cggtactggt cgcaggaaaa acgccattgc gcgggtacgt
     7501 cttgttcctg gtagcggtga aataaccatt aatagcagag acatcgatca atatttcggt
     7561 ttggaaacat taaaactaat cgtccgccaa ccattaaagt taacggatac tttagagaaa
     7621 tttaatgtga ttgccaaagt cgaaggtggc gggactacag gtcaagcagg tgcaattcgt
     7681 cttggtattg cacgtgccct gcttaaagca gaaggcaatt tacgtcctgc actaaaaagt
     7741 gctggcttcc tcacccgtga tccacggatg atagaacgta aaaaatacgg tctcaagaaa
     7801 gccagacgtg cgccacaatt ctcaaaacgt taattttaag cttaaaatta gcatataagt
     7861 ttaatcacca gtttttgtgc aaacagaaac tggtgatttt ctatactttg atagatccat
     7921 catctctctg caaggaggcc cgtttgctgt gaccaccaaa caacagttca tggccggtgc
     7981 tggcaaagcg atacccgtag ttctaggtta tttacccctg ggttttgcct atggcgctct
     8041 agccagcctg gccggcattt ctttgcctca tatcatcttg atgtccctgt tagtgtttgc
     8101 tggcagtggc cagtttatcg ccgtgaacat gtttgctctc ggcgccggtt ccctctcgat
     8161 tattgtcact gtttttctgg ttaacttacg acaccttttg atgagtgctt ctctttcccc
     8221 ttatctgcag aagtttccgc gtcaatcatt accattgtta gcttttgggc taaccgatga
     8281 gaactatgcc ttggcctgcg gtgaattaag taagcaggaa agaaatcata actttctgct
     8341 tggacttttt gctacctccc atgccggctg ggtctgcggt ggtgccttag ggggaatctt
     8401 tggccaactg tttagcagtg ctcgtgctgc ttacacgatg gagtatgctt tatatgccat
     8461 gtttattttt cttttagtta tccaagtgac agataaaaaa ttattgttag cagcctctat
     8521 ctctggtgtt ctttcattat tattttatca attgattccc ggcagttggt acattatcct
     8581 ggccacagta gcgacggcca ccttaggagt gatgattgaa aaatggcaag ggtctttatc
     8641 ttaattttag gcatggcaat tgtaacttat ctgccgcgtt ttttaccgat gtatatcctt
     8701 accaggctgg aaattccgca agtggtgagc acttggttac gctacatacc tgtagctgta
     8761 ttagcagcgc tgattgttcc cggcatactt gcagtaaatg gtcacatcac cgtctcacca
     8821 aataattatt acctgttagc cagttttctc gcttttgggg ttgcctatcg ctttagaaat
     8881 atgatgctga ctgtaacagt aggcatggct gctgtgctca tcctacaaaa tttatggtag
     8941 gtctgctact tctattcttc attccgcaac ttacttacta atctttagca gctcttgcca
     9001 aggatgcaca caaaaaggtt taattaaata aaaagatgca gtccctatgt tggggctgca
     9061 tctttctaga ccggcaagga aattatattc taacttggtt ggccaaatag tgcctcaaat
     9121 tcaaaggtca acccggggaa gctggggtga gtgaaggtac ccttgccggc cctgaccatc
     9181 gatacataat gttcatcacg taattcgtag gcttcaataa agcaattttc aggatctaat
     9241 atccaatagt gaaggactcc tgccgcttga tagtggttta acttgaggac gcgatctttt
     9301 ctgctggtgg aaggggacaa aacctccact actagttccg gtacagtctt aatcggggta
     9361 gcttgtgcag gactgctgcc aggcaagtac aacaggtctg gctgtaccac attgtattca
     9421 gccagagtta gatcaagggg agcgttaaaa acctcaccct tggggtctac taccgcaaag
     9481 taatcttcaa gtattcgctg cagcctgcgc gaaactcgtt ggtgctgaaa agagggagag
     9541 ggatcgcgca gaagcacccc gtcaatcacc tcataacgaa aaccgggatc ttcaggaagg
     9601 ctggcgtagt cttcgtaagt gagcttcttt ttgccctcgt aacgtgaaga atcctcagct
     9661 acagctgctt tccctgctaa agctcttaag acactctgtt tgtgataccg gtactgtctg
     9721 ttaccaatct ccacaaaagg tattttcttt tcccttgtat accgccagat tgtatccacg
     9781 gaaagacata agatcgctgc taactcttga gcagtaataa gttcattttc ttgcacttaa
     9841 aacacctcct atcttcataa tatatagcta taataacaat tgtcaactat aatcaactca
     9901 atatggtctt gggcaacatc aatatcttaa ctctaggcaa ttatagtaac tgcgtaagag
     9961 tattctgaag ttctcttgtt tgctatgcta tgaaatttat ctggaggaaa tggcggctat
    10021 agaataatcg ttgtataaca tatataatgc tcttagacgt atactagctt tttatgaagc
    10081 ctatcttacg aaataaaata gacaactgat aggaggctaa tcatgagcaa aacttactgg
    10141 ggtgacatta ctgcaatcgg cagtttaccc gatattggca ccgctgcagg tttagagtta
    10201 gtcttacaca gcttgaaaca tatccctcac tggccgcaat tccctaaacg atcgcaagag
    10261 tatctcactc atcagttttt gcaggtacta gtagattgtg gcctgatgga agtacctgaa
    10321 cagggtaatc ccgtgctcaa cgaacaaacc cccacctttc ttgaaggact aactgctttc
    10381 taccaaacct acttagaagc tgaaagcggc gatctagagg cacttgcatc ctttgccatt
    10441 ccggattggg cgggtgaagg ttttgctgct ttgattaagg agttagagaa taacccggcg
    10501 ctccaaccgc aatatctcaa aggacagtta attggcccgc ttacggccgg actgcagatc
    10561 atgaatgctg acaatatccc cattatttat gatgatcagc tgcgtgatgt ggtaattaaa
    10621 gcgttaacca tgaatattcg ctggcaggtc agccagcttt cccgcaccgg gctgccggtg
    10681 ataatctttt tagatgaagg gctgcttcat gcgtatggcc accgtgattt cctcggtctt
    10741 agcgcccaac taattagtga tacttttacg gaattaatca ccgccattaa agatagtggc
    10801 gctgaagcag gcatccactt ttgttcccgt gccgactggg agttgttatt ccctgcccag
    10861 ccagatatcc tggcctttga tgcttattcc tacttttcgg ggatgctggc cgcagcagaa
    10921 caagtgaaca cctttttagc acaagggggt actattgcct ggggattaat tccttcttac
    10981 gaagcctgtt ttacagaaac agctgccagt ctctctcaac gctggcggga ttacattacc
    11041 cgtctggaac aaaaaggagt cgacggtgct cgtttacgca aacagtatct catcaccccc
    11101 agctgtggta ctggttttta taccccggct attgcccagc gagtttatca actgctgcag
    11161 gaaacaggag acttaatacg tcagacgtgc tttgattttt aagcgtgata cgcctttaca
    11221 ggaaaatata gggttctctg cagagcagaa gaaagcggct tttatgccga cagcgatgca
    11281 aaaaaaaacg gctcctgtaa gcacaggagc cgctactttt gctaattgtt ttcttctctt
    11341 tccagaaagg ttctcacgac catagaggca tgaaagaagg tttgcgcacc gtaaaaggat
    11401 gccaatgaac aggccagcat gcctacttta ccaaggtatg aattagctaa aaacctgctg
    11461 atagggaaaa gggctaccca tgcgtccctg ctctgttttc atcgtctcgc tgaagatctt
    11521 tttcatctgg tcatcccctt ttctacaccg aatgacaggg gtgagaagat cgatctagtt
    11581 tttatttcca tgctaattgt atcaaaagct tcataccttt catagtggca aagcaatccc
    11641 aacagaacca gctagacaca gcattgagaa atatgtgcag cgtctttctg gtataataga
    11701 gccagagcaa gtgctcgcga agaggagggt caggctggtg tgtgaatttt gtacccaaca
    11761 tggtgaaggt aaaaagtggt atgaagtaat gcagaactac tcaggagagc tattggccaa
    11821 tgaaggacgg atgcagtata ttcgtcactt tatacctaac gtacaaaaaa atgctcaggc
    11881 gaatattgct aaattagctt gggccaagca gcattttccg gctgtttatc gttttatccg
    11941 cagtattggt accaagcgcc tgaaacagat tcattttgga caagttgtgc cgctagaaga
    12001 tgcagaaaaa gtcattgatc tggtacaatc aattaccaga acagcttgtg tctgtcgcag
    12061 tgtagtttca ggtaaaaaga atgctcgcta ttgcttgctc cttggtgttg atccctatca
    12121 gtccggcatt gattatgccg acttacagca cagtttggag acaattacgc ctacggaagc
    12181 taaacagctc ctgcgtgaat tcgaccagca aggtttagtc cattccatct ggaccatgaa
    12241 aacaccattt attggtgcgc tctgcaactg caatcaagaa tgtcttgctt atcaggtaca
    12301 agttgaaagc aagttgttag agatgatgtt taaggcagaa tacatagcag aaattgaggc
    12361 agctctctgt acgggctgtc gccgttgtca gcagctctgc cagtttgagg ctattgctta
    12421 cgagcacggt caatgctcca tcaatacaca aaaatgctat ggctgtgggg tatgtcgtca
    12481 agcgtgtcct gcagaggcaa ttgtattgcg gccaaagaag atcatcgcca gcggctgccc
    12541 tgaaatcgta gagacggaaa gggcacatag ataaaggcaa gcttaaatta aggaatgtga
    12601 tgttatggat aatctgcctg aatatctgga tgataaccta aaagttgttt ttgtcggtta
    12661 taaccccggg gaaaaagcag cggctctggg gcaccattat gccgggagag gtaatcaatt
    12721 ctggcgtctg ttatttgacg ctggcttaac taaccgcctt tatctgccca cagaagatgg
    12781 gaaactactg cagttaggtt acggtttgac taatatcgtg tctaggccta gtcgcagtag
    12841 ttcagagctg cacaaaaaag aactgcaggc aggagcggaa gtgctgcggg aaaaactgcg
    12901 ccgctttcaa ccttacctag tctgtctact gggtaaggaa gtctaccggc attatgcggg
    12961 gctgaaaagc agtgcctctt atacttttgg cgccaaagct acagcgacta tccctggcat
    13021 caaggagttt gctgcaccga accccagcgc gcgcagtaca attccctatg gggtcaagct
    13081 cgcttatttc gtgcaaatat ctcagctgct tcgctcacga taacaactat catggtctga
    13141 agtgtgggcc gtacaaatag aattaataag agccggaatc gctgtccaag cgattccgcc
    13201 tctttggagc gctataggta tactggacac gcataacctt ccctttacgc tggtgtcctt
    13261 atgattacta atttattcaa ctaatctcat taggctgcgc agtagtcgct gtttaggata
    13321 agcgggatat agaaatcgta catctgccaa aaggatgaga ttgtaagaaa ggccaaacca
    13381 actaaaatag tccgtttata gtcaagtttc atttgcttca cctccgaaat tgaaccctaa
    13441 tttaaatgta tcacacaata acaacttagg aaactgggac tttacaagag gggggtgcac
    13501 ggcaatttga ctccgcaggg cctgaacctt atttcctttt tgacttatgc agcttggcaa
    13561 attgtctgcc ccattgcttt tcctgcaaac gcaatgttcc ctctctttta gcttcgaggt
    13621 gagttttttc cttttgcagt ttcaaccaac tttcccacct tggtaccatc aatgtttccg
    13681 cctctagggc tgcgcggata gcacatcccg gttccgtttg atgctggcag tcagaaaaac
    13741 ggcagtgctg aatcaattct tccacatcac caaacatgat ttccatgcct tgttcagctt
    13801 cccagaggga aagcgcacgc atgcctggcg tatccataat cagaccaccg ctgggcagca
    13861 gcaccagctc tcggtgggta gtggtgtgtc tgccccggct atcgttctct ctgatggtct
    13921 gtgtagaaaa ctgttctttg tttaacaaca ggttaacgag cgtagatttc ccgacaccgg
    13981 aggaacctaa taaggttact gttttgccag gcgcaaaata gcgctggaga tctttgaggc
    14041 ctgcttttgt taagcagcta atcgcatata ctgccacacc caaagcggta tcctgcgctt
    14101 tagcgacttt aactgccaca tcagtacaac aatctgcttt cgtcaaaact acaacaggcg
    14161 cagcaccact ttcccaggcg gcgattaaat accgttccaa tcttttcatg ttaaaatctc
    14221 tattcaagga ttgcatcaga aaaactgtat cagcattagc ggccacgatt tgttctttaa
    14281 cggcattgcc agcggcagcg cgcgagaact tcgtttttct cggcagtaca aaacgtactc
    14341 ccactgtgtc ctcactcaac tgctctaaag caacccaatc accaacggca atcgatacag
    14401 cctgattttc cttcgttagc ggtctagcta cttcaagttc cccaatttcg ctgattaggc
    14461 ggagtttctg gccataatca gccaccactc tgccaggaaa catctcgtct ttagcctggc
    14521 tatgccaatg ttctgcaaaa tatgcatccc aaccataatc ttcaattagc aagatattta
    14581 ccttcctttt ttagcaatct gtttttggct ataaaaatac cgcagatatc gaccatctgc
    14641 ggattggttt aaagtaaaag ggcaacaaaa aaacacgcca cctctgcgtg ctttcacatt
    14701 gctgaacacg atccggtttg ctcgaataag ttgcacagat aacacaaaca agcagtctgt
    14761 gactgtgccc gtatatagtt agctaaaaag cacctcagca gagaatacag tcatcaacaa
    14821 cacatccttt ctcaagcttc cagcatcacc tttattatac aacaaatccc tggaaagtaa
    14881 actgattttc taatttaact ttcactctct gccttagcag taggcttatg gctttagatc
    14941 gtcgcaaaag cagagtatta aattcgttag gaggataagt tgccataaat aaattattat
    15001 agccaatttg cagcagacaa gggttgctgc ctccataggt aattgtcttt acttaatggg
    15061 ggtttctttg atttttatca gtgaaacacc tgggatattt tatcattgac aggataatcc
    15121 ctaacctata caatgtcagt agagcagaaa tttgatgaat tttgtcagat ttgctcgaac
    15181 tgtaggggtt aaacctattc ctaaatatga atcgagcggg aagggaggga caatcagtag
    15241 ggctgttgta gtgatgattg tagctgcttt gctaatttgt tagactcaaa agctcttccc
    15301 cttttatggt aatagatgag taaagtatca ggatagtaaa ccattaggaa taaggcctgc
    15361 agtcatgaac aagtacatgc aacattcgtg tgatttatgt tagtactttt tatttactat
    15421 ctcaagttac aataatcaca aatgctagtg actggtaacg gcagtccttt ttttgggcga
    15481 atgatgtgaa aaaaatcaca tcattctgcc ttccccgggc aaatagttat ttctatgaaa
    15541 ggagagagct gatgaaaaaa aagcaagtgc tttctctgac acttttaact ttagtgctca
    15601 tattggcaat gattttactg gctggctgtg gtggtggaga ggaaacagaa gcaccgccga
    15661 tcaatgaggg agataccccg ccggttgatc atgtcgtcac aatgcctgat gattatgaaa
    15721 gcactgatta ctgtctcgaa tgccatatca ttgaacctta tgtagaatca tgggaatcgt
    15781 catcaatgct tgctaactca caccaacaag ccgggattaa ttgtgtaaat tgtcacgaga
    15841 gaacagaaga aattactgca caagaaatcg aaaagtataa caacggtgac tacatggatc
    15901 ctctagttag cacacagatg cctatggagg attgtctgca gtgccatgag agctatgaag
    15961 cactggcaga aagaacagct gattatacgt atgaggggga acaggttaat cctcatgatt
    16021 ctcacttagg tgagcaggag tgctcctctt gccatactat gcaccaagaa tccagtggga
    16081 tcaattactg cctcagctgc caccatgaag gtggtctggt tagctgctac gaatgccatg
    16141 accatattta agagtaaagt aagcaggtat acagttataa taattacagt aactgaggag
    16201 gttaacaatg tctaaagaga ctaaagaaac taaaggtacg aaacgttccc ggaggcaatt
    16261 tataaaggat gccgcgctcg tagcaggtgc tactgctgtg ggtggatttt tcctcactgg
    16321 ctgctcagac ggagctgctg aaggcggcga agaaattacc tgggataagg aaacagatgt
    16381 tctcgttgtt ggtacaggtt gtgggctagc aggtgccatt gaagctaaga atgctggcgc
    16441 tgatgtcctg gttctggaac gcaacgactg ggcaggtggt ttatatatca cgaccggtgg
    16501 acatgccatc ctaggcggaa cacacattca aaaagaagaa ggcatcgagg atagcattga
    16561 agcttggtat gaagatgaga taaaatctgc cggacatcgt ggtgtgccgg aacttattcg
    16621 cacttatact gagaggggtc cagagtttgt tgagtggatg gagtcaatgg gcttcaaatg
    16681 gcaaaagccg ctaggtcatt ccggggcgga agttcatcgt gtaccacgtt cacattatgc
    16741 aggtagtaat cctagcgttt atcaaagcca gacgggtgca gccagaagtt ttggtttagc
    16801 ttggacaact gtatggatga agaaaatcga agagttgggt atacccatcc tctataagca
    16861 tagaatgaga aaaatttatc gcgaaaatgg tggtccggtc attggtgttt cctgtgatac
    16921 gccggacggt acagttaata ttaaagcccg tagaggtgtt gtactaacta ctggtacttg
    16981 gactgataac tatcgcatgg tacaagcttg ggatccacgc gctgttggac cagattgcta
    17041 tggcgacggc ggaaccccct gcgacggtac attatttgtt gatagctctg gagatggcca
    17101 tttagcagcc gcagaagttg gcgcagggtt tagtgatatg tcgttcgtgt catactacta
    17161 tctcttctat ggctccagat catactgggg ctgggagccg ccagatttta aaacagccga
    17221 ttactatatg gctggtaaag gcctcacgcg caacaaggct ttctttgaac gtgttgtcct
    17281 ggttaataat gatggcaagc gctacattaa tgaaatggaa ggcacccgtt cttctgatcc
    17341 cgaactaggt gaagctggcg gcctcaatga gaatcctgaa tggccgtata ctgccaagta
    17401 tcttgcgctg ccgcagccga gaaacgtctg ggcagttaca gatgctgaag gcgcagctgc
    17461 catgaagtgg ccgatagagg atctagagaa tcctaatcct aaaaaaggtg ccatgttcga
    17521 tccggcatgt attgccatcg ctgatagcat tgaagagcta gccagcaaaa tgggcgttga
    17581 tgtagctggt ttagaagaaa cgctcactcg ttataacggt tttgctgatg caggtgagga
    17641 tgaagacttt ggcaaaccca tgccgctgtc caagattgct actccaccct tctacgcagc
    17701 taaggcttcc ttaatccgtc atacccagcg taatggtatt cgcgttaaca ccaaagctca
    17761 agtgattgag caagcggatc aggttgctgg ttacgatggt actgctagcg gtaagagcat
    17821 ttccattgat gatgaaaaag ttatcccccg tctctatgct tctggtgagt tagccaatat
    17881 tatgggttgg agacgtgcgc atggttccat gggtaactac gcaatatttg cccgtatcgc
    17941 tggtgaacag gctgccaaag aagaaccatt ggaataaatc gtagaaagaa cgattaaatc
    18001 ataattaaac ggaacacaga gtggcgggga cgggtctgcc cgttccctgc ctttataatt
    18061 attcttcttg cacaataagt aagtaagcaa tataattatc acgtggaggg acttatgacg
    18121 gagaatgcta aagtagtggc tgaaaaagac ccagtacacc cacaccacat agcagttgag
    18181 atggctaaag ttccacctgc taatgtttat gcaggcgttt ttatgacctt gaaggttaaa
    18241 gtctcttgta ctgcagcttg cagcctcaag gggcagttag tgctaatatt agatcatact
    18301 gatcaagtgg taaaagaatt gcgattgatt acaccatatc ggggagctta cgagacagat
    18361 caatttgtcc tgcaggcacc gcacaatccg ggcgaatata cctggacagc tgttttcccc
    18421 cagcaggagc gggataatgt tagccataag cgcgctgcgg cagactttaa ttttactgtc
    18481 aaggcgcacc atgctacaag tatcgctgtc tgggacgtca cttcgccagt agcaatcaag
    18541 acagcaagta gagtaaaagt aggtgttaaa tgctctgtcg gctgccagct gacaggcagg
    18601 cagatagaaa tttataacca gagtggtcac aaggcagcag aagccacttt aggcgatgtg
    18661 ccctggaaag gtacagatag catgtactgg acagaaatca ccataccggc tccggacacc
    18721 acaggttatt ctacctggca agccagattt tctgaatcag agctggaaac acatttggct
    18781 aagcaggcta gttttggttt tacagtagtt aactcacctg aatgtagtat gacggttcag
    18841 gtagttgagc gggaaagcaa agttcccgtc agtaatgcga gtattattat gcatccatat
    18901 cggattaaaa ctgatgaaaa gggcagagcc actttagccg tagctaaagg tgaatataaa
    18961 ctttttgtca ccagagaaga caagtatgag ctatggcagc aaacagtgca agtaaccgct
    19021 gagcaaacta ttacagccga gcttgcggtt actccgccca tcgtacatga gggataactt
    19081 ttacaaatat ataattggca aaatgaaggg ggaaaaataa tgtttggtgg taaacttggc
    19141 acacaggaat taattttaat tttgggtgtg gccctgcttg tgtttgggcc acagaagctg
    19201 cctgaaattg gtaaggctgt cggtaaagga ctgcgagagt ttagaaaagc tacaaccgaa
    19261 ttaaagaaca gtatcgatag tgatgctgaa cagccagcac ctgctcccaa gcaagaagtg
    19321 ccggcagaaa cagtagctca aactccgaac gacgtagtta ataaagaata gcagggagga
    19381 ttatcatgaa gataggttgg cttgaactag gcttcattct tttaatcgtg ttagttgtta
    19441 tgggtccagg gaagtttacg ggaatattta aagggattaa aaaaggtgtg tccaacttca
    19501 gagaatctgc ctccagtgat gatgagcagt aaatagaaaa acaacaagct aaggcagcaa
    19561 atgggggaac gctatgggta caaaaaaaga tgtcgcagag atgacgatct gggatcactt
    19621 gggtgacctg cgtaaaagat taattattat ggtcataact gtgttagtgg caactgtaat
    19681 cagttttgcc tttattaacg acattctgca gtttttaatc aagccagcac caggtctgca
    19741 gctaatcttt acctcccctc ctgaagcttt aacggcacag attagattag caattcaggt
    19801 ggcgttgatt ggcacgttac cactaaattt agttcaactt ttaattttta tcatgccggc
    19861 cctcgagaag accgaaaaaa aggctctgct gccttttttt ctcgcgatga atctgctttt
    19921 ctttttaggc gttacgtttt gttattacct agtgatacct tttgctatta agttttttat
    19981 gggctttggt acagaatctt taactccttt attttccatt ggtaattacg tttctttcat
    20041 cacttcactt ttattatctt ttggtgctat ctttgaaatt cctttagtct tttggttcct
    20101 gggattaatg ggccttattt cttcagattt tttacgtaaa aaccgcaagt ttgctgtgct
    20161 cgtgattgtg attgtcgcgg caattatgac accgccagat gtcgtttcac aaattcttat
    20221 gtcgattcct ttattatttc tttatgaggc aggaacgctg ctcgttagat taacagaacg
    20281 tcagaaagca aagagagcag cttaaagagc aaaataaata tttatcatca taacagccag
    20341 tacgtcttgt actggctgtt ttagcgtctg tcctacacag aaattagcaa aaaaaatatg
    20401 atagctgatt tatcgctaaa aatcttaccc tgtaagttaa ttatcataac ttatggggtt
    20461 ttccctgatt tatactggta aacattcggg taatctttgc cattgacagg ataatcctta
    20521 cattatacaa tatttgcagg gcgtaaattg atgtatcttg tcaaattagc tcgaattgta
    20581 ggagttaacc ctaatcctag gtatgaattt agtggggagg gagggaaaaa ctgccgggct
    20641 gttgtagtga tgattgtagc tgctttgcta attttatagc ctcaaaagct cttccccttt
    20701 tacggtaata gatgagtaaa gttctgggat agtaaatcac taggaattag gcctgcactg
    20761 ctgaaaagtg catcaaacgc ccgtgcgatt tgtgcgaggg cacttctatt tactacccat
    20821 agttacaaaa ttcacaagtg ctaaccgcag aaagcaacag ccttattttt ggcaggaatt
    20881 aaatcgacaa gctgaggggg gaaaatttat gtttggcggt agaattggta cacaggaatt
    20941 aatattaatt ttgggtgtgg ctcttctcgt atttggccca cagaagcttc cggaaattgg
    21001 taaagctgtg ggtaaagggt taagagagtt taaaaaagct accaatgaat ttagaagcag
    21061 tattgatgtt gatactgaac agccagccgc tgagcctaaa caggatgtgc cggcacaacc
    21121 tgtagtaaat gctacggatg aagtcgcagt tagtaatgaa tagcaaggag gattaccatg
    21181 aggatcggtt ggcttgagtt aattgttatt cttttcatat tgttaatggt aatgggaccg
    21241 ggaaaatttg ccggcatctt taaaggtata aaaaatggtg tcgcaaactt caaggaatcc
    21301 gtttccgatg aagaagagca atagcagtgc aataacaatg ccatttatac ttgctatagt
    21361 accgcttatt tgagttatca ctataatttt tataggaaat aggaggaagt ctagtgaaag
    21421 aagaaaatag aaaaggaact ctagtagagg atgctgtcaa gggcattaac cggcgaaact
    21481 ttctaaaagg tgcggcggtt agcgcgttga gcgtaacgtc actggggatg cttgctggat
    21541 gcagccccct aaataaagag gatgagtccg cgaatgatgc gaagaaagtt gtttatgaac
    21601 tgcccgaccg tgaaggtaag ctgacactgg aggagcttaa tgcacttcgt cagcagtacg
    21661 ttgactcaaa agaagaatat gtctgcgaag atggtactgt tattcctgcc gtttacgtaa
    21721 agctccgcgc cctgattaat acgtatggca acggcgcggg ctcagaagtt cacgatgcta
    21781 gttttaacga ggttatgtac ctctttagcg agaaagaagc ccaggcctat ctcgagatgc
    21841 ccttcggcgt gcgttttaca gcaaccgatt tttcggttaa atcgggtcgt gacgagactg
    21901 attgtctcaa gctctgtgaa gatcttgctg ctcgtggcct actgaagcgc ctccgccgtg
    21961 gcggcgttgc ttacttccat catctggcac cggcccatgg tatttgggaa tacgttctat
    22021 tactgccaga aaaccaaaat aaagaatatg ttacgaagca taagtcgatg tggggcgctg
    22081 atatcgtcag cgatctatac gatattgata cccccttcta ctatccgatc ccggtggata
    22141 aggagatagt ggtagaagat gaggtgctaa gttatgatga ctacgaaaaa attgtcgctc
    22201 ggaacgaggt catcgctgtc tccacctgcc agtgccgtct aggtcaagat atagatggca
    22261 gtagagcccc tgattgcgat catccgatgg agacctgcat taccaccggt gagcaggccg
    22321 agttttatat tgaaaacggt atcggccgtc agattgatca agaagaggct ctcaagattt
    22381 tgcgtcatag cgttgaagta ggtatggtgc tccagagctg ccatacgagc tccactgaag
    22441 ttatctgttc ctgtcatggt gactgctgcg atgtccttaa gtcttatgtt gcacttggtg
    22501 agaattatga caagcttggt atttcggagg ttgtcagcca ctatgaactt cagtacaaca
    22561 gggatgagtg tgtaaaatgc cgtctctgtg agcctgtctg cccgatgttc gctatcacca
    22621 tggacgatga gggttactca gtgaccggtt ctccttgtat cagctgcggt caatgtgcta
    22681 cagtatgtcc ggctggcgct cgtaagctgg tccagcggga aggtgcagac caaaccaaac
    22741 ttccaggctc attgcttgat gattacaacc ttaaggctag ctaccgcttt aaaaataaca
    22801 tgattagcta aactaggatt aattacgata aatgacctgc acaggcggga agatttagag
    22861 ctttccgcct acctgggtca agcgagataa gaaggggtgg gaaaatgcat gaaatggcgc
    22921 ttacgggcga cgtggtggac attgtagtca ataaggccga agcggttgga gctaccgagg
    22981 tgcgcgcggt gtacctgacc attggttatg tgcgtgacat tgtagaagat attttcgagc
    23041 gttgttttgc ttatctggcg cgtaacacgg tggccgaaaa tgcggagctg gttatcaccc
    23101 gtgtaccctt caccgtgcgc tgcaacaatt gcgagcacgt ctatcacctt gacgttcatg
    23161 acgagcttac gtggatatgt cccgtttgca acgcgaaaga ctataagctt catacgggca
    23221 tggagttttt cgtcaataat attgaggttg ttggacctgc tgctcctgaa ggccaggaag
    23281 atcaggcctg cgcgcaaaaa taactaatag gtgatatctc tttggcgaga gtcccggcgt
    23341 taggaaagta cttttgttgc tgacgtcgag agagataaac gtgaaagtga ttggcccggc
    23401 taaaaccgaa cagtgataaa acaacaggag gggtgtttca gatgaatgag ctacgtacgg
    23461 tagagattaa acaagaaatc ctcgccgata actcgcgtga ggcggatgca tttcgcgcag
    23521 cgcgacgcgc cgagggaacc tttatgatag atataatggg atcgccggga gcgggcaaga
    23581 cgacgcttct gctcgcactt atcgagcagc tcagaggcga gagcggtgct gaaatcggcg
    23641 tgattgaggc tgacttggca tctgatgtgg acgccctcaa aatcaaagag gctggggtag
    23701 cagccgtgga gcttaacacg aaaaacgtgt gccacgtaga gatgggaatg gtagagaagg
    23761 ccttcgcggc ctttgctggt aagaggttcg actatgtatt cctggagaac atcggcaacc
    23821 ttgtgtgtcc agcggagttc gacacggggt gtcactccag gatcatgctg ctgtctgttc
    23881 cggaagggta tgacaaggtc tacaagtatc cgcctatgtt cgcggtggtg gatgccctcg
    23941 tagtaaccaa gtccgactac ctgcccctga accctgactt tgaccgccag gccttaagag
    24001 aaggagcgcg tgcgctcaac ccgaaacttg aaatcttcga agtgtcggcc agaactggtg
    24061 aaggaatggc tgaacttgct gcgtggttcc aggcccgtcg cgaagccgca cttaatttag
    24121 cataaatacc gagaacgaat agaaaggaag ttgcttatgt gcacaaacag aatcaacaca
    24181 aatcagctcg aacagatgat tgaccagatc gatgaccaca tcgccctcga gtaccgctgg
    24241 gttcacaagc ttgctcacgc ggcggacgac gaaggctttg ccgcggcctc ggagaagctg
    24301 catgccgccc agctcctact tgctgacgtg cgtgctgcgc tcgacgaagc gaaagaagcc
    24361 ctcgagtcat ccgcaagctc tgcaagcgac acgaccgccc gccttgttta gggacgaatc
    24421 ctacactcga gtcgcttcga ttataagtta ttaggcagcg tttcgctgtc tttgaatata
    24481 aactttttgt caccagagaa gacatgtatg agctatggca gcaaacagtg caagtaaccg
    24541 ctgagcaaaa tattacagct gagcttgcgg ttactccgcc cattgtgcaa gagggataac
    24601 ttttacaaat atttaattgg caaaatgaag ggggaaaaaa taatgtttgg tggtagactt
    24661 ggtacacagg aattaatttt aattttgggt gtggccctgc ttgtgtttgg accacagaag
    24721 ctgcctgaaa ttggtaaggc tgtaggtaaa gggctgcgag agtttaaaaa agctacaacc
    24781 gaatttaaga acagtattga cattgatgct gaacagccag cacctgctcc caagcaagaa
    24841 gtgccgacag aaacagcagc tcaaactgcg gacgaaaccg tagttaatca tgaatagcag
    24901 ggaggattat catgaggata ggttggcttg aattaggctt cattctatta atcgtgctag
    24961 tagtcttggg tccagggaag tttactggaa tatttaaagg gattaaaaaa ggtgtgtcaa
    25021 acttcagaga atctatctca agagacgacg agcagtagac agaaaaacaa caagttaagg
    25081 cagcaaatgg gggaacgcta tgggtataaa aaaagatatc tcagagatga ctatttggga
    25141 tcacttaggt gacctgcgta aaagattaat tattatggtc ataactgtgt tagtggcaac
    25201 tgtaatcagt tttgctttta ttaacgacat tctggagttt ttaatcaagc cagcaccagg
    25261 cctgcaatta atctttacct cccctcctga agctctaacg gcacagatta ggctagcaat
    25321 tcaagttgct ttgattgcca cgttaccact aaatttaatt caacttttaa tttttatcat
    25381 gccagccctc gagaatacgg aaaaaaaggc tcttctgcct tttttcctcg ctatgaatct
    25441 gcttttcttt ttaggcgttg cgttttgtta ttatctggtg ataccttttg ccattaagtt
    25501 tttcatgggc tttggtacag aatcgctaac tcctttattc tccattagta attatgtttc
    25561 tttcatcact tcccttttac tagcgttcgg cgctattttc caaattccct tagtcttttg
    25621 gtttctggga ttaatcggcc tcatatcttc agacttctta cgccgaaacc gcaagtttgc
    25681 cgtgctcgtt atcatgttgg tatcagcact tatgaccccg ccagatatca tttcacaagt
    25741 tctcatggcg attcctttag catttctata cgaagcagga gtgctgctag ttagattaac
    25801 agaacgtcag aaagcaaaga aagcagctta aagaggaaac taaatatcta taatagcaac
    25861 agccagtacg ccttgtactg gctgttgcag cacctattat cctcattgtt caagctctgc
    25921 aaatgaaaaa acttcttcaa ttgtactgtg taaagcgcgg gaaatcttat aggcgagaat
    25981 taaggacaga ttatatttgt tattttcgat atgcccaatt gtttctcgtc gcacattgac
    26041 tagctgcgca agttgttctt gagttaaatc atatctggca cgaagttctt ttatgcgcga
    26101 cttaatctta acttccacct taagactcac cccgcttctc agacacgtta aaagagatca
    26161 caaaggtaat aatctgcaca acgaaagtga cagcaataaa catcacagct gcttctaaag
    26221 tcttgaccac tgccacgccg gcaatggccg ctgctaggct ggccagcgag acaacgaagg
    26281 cattaaagcc tgctttgtgc aaattttttc ttaacaattc atcatctttg atcttggcca
    26341 tcccccaaaa gccaaaaaaa ccaaagaagc caaagaagcc aacattatct gtaaatatac
    26401 ccagcaagcc aagcagaccc agccaaccaa acattttcag tatcttcaca agcaaactcc
    26461 tttcgctata aatacataac taaatgttat atatttatat ccttatgtga taaatatata
    26521 acaagatggg caaaaagtca aatcctaaaa gcagtttaaa gaaactttta tcgctgtagt
    26581 ttggattttc gatgactgat tgacaattga gcgagcgagc aggagttaaa cgattgtttt
    26641 gatccttcaa ttcatctcag ccaggttgat cagatatttg cccgggtagg catttaggtt
    26701 tatgagctca ctaatggtat aataatccta ataactgcgg cgaaagcgta gaaacagaat
    26761 ggaggattac catgacaaag gtgcaagctg tcaagagact tcccttaaga aaggaaattg
    26821 ccgaaaaaga cacttggcgg attgaagata ttttccccag tgatgaagcc tgggaaaagg
    26881 aatttcagga agtaaaagca atggttccag ggattaaaga ttatcagagc agactggatg
    26941 aaagtgcgga aaccctctat gcggcactgc aagatcagga tcaattaaca ttgcggctag
    27001 gcaagcttta tgcctatgca catctgcgtt acgaccaaga taccaccaat tccatttatc
    27061 aggcgcttaa cgagcgtatg agaaatctat atgcccaggc tgccagccag ctcgccttta
    27121 ttgtgccgga actacttagt atagcagaag aaaaactaca ggcattttta gagcaaaaag
    27181 cagaactgcg tctctatgag catgctttgg cagaaataaa tcgccaaagg ccgcacgtgc
    27241 tgccagcagc acaggaagca ctattggccg aggcctccga agtgctggga gcttcacaaa
    27301 atacttttgg tatgttaaat aacgctgatc tggaattccc tgtgatcaaa gatgacaagg
    27361 gagaagaagt agaggttacg catggcagat tcatccgctt tttagaaagt gccgaccagc
    27421 gggtacgcca tgatgccttt aacgctgttt atgataccta tggcaagttc cgtaatacct
    27481 ttgccagtac tttgagcggc aatattaaga agaacaattt ttatgcccgc atcagaaatt
    27541 atgcctcagc cagagaagca gctctggcca aaaacaatat tcctgaaagt gtctatgata
    27601 acttggtgag cactgtaaac gagcatttac cgctgctgca tcgttatctc aagctgcgta
    27661 agcaagttct agggcttaac aaactacata tgtatgacct atatactcca ctggtcaaag
    27721 atgtcaagat ggagatcggt tatgcgcagg cacaagacct gatcatggca ggcttagccc
    27781 ccctgggtga agattacctc aaggtgctgc aggaaggttt tgccaaccgc tgggtagatg
    27841 tacaggaaag taaagggaag cgcagcgggg cctactccag cggcgcctat ggcacaaatc
    27901 cctacattct gttgaactgg caaaacaatc tcactgactt atttaccttg gcacacgaat
    27961 ttggtcattc agtgcacagt tactatacga gaaagtatca accctaccca tacggcgatt
    28021 atgctatttt tgtggctgaa gtagcctcga cctgtaatga agccttacta aatgactatt
    28081 tactccacac catcactgat caacaaaaac ggctctatct cttaaatcat tttttagaag
    28141 gcttcagagc tacagtattt cgtcagacca tgtttgcgga gtttgagcat cttatccatc
    28201 aacaagcgca gaacaatgaa gccttaacag cagaatcact caccaaagat tactatcagc
    28261 taaaccagaa gtattacggt acagaggaca tgatcattga tgagcaaatt ggcctggaat
    28321 gggccagaat tccccacttc tattacaatt attatgtcta tcagtacgct acaggctaca
    28381 gtgcggcaac agcccttagt aaaggaattc taaccacagg cacaccggca gtagctaggt
    28441 atctagagtt tttacaagcc ggcagctctg attatccgct tgcagttttg caaaaagccg
    28501 gtgtggagat gaccagtaaa aagcccattg aagcagcctg taaagtattt gccgagaaac
    28561 tgacagaatt tgcacaactg gcagggatac aaacgagcta aaccctgtcc atttccccaa
    28621 acagaaactt gagacacctg atgtaatgca acccaaaggg attgatgatg ccactaactg
    28681 cagtttttgc ggcatcatca atcccttgtc tactattctg tataacttcc ttttgctgct
    28741 gagatgtgag tatagcacgt cttagggtgc tttgaggatc acgagcagct gtttctgacc
    28801 aaattcaatg gattcctctt tcgatttgca tgctagatct atatcgttag gcccgatatt
    28861 gccaccgcga tcctctacgg cataccaacc ttctccttcg acatatattt ctgtgccgaa
    28921 aggaatgttt tcaccggcag ccgcagtctt gccgggaacc agtttctcgc cactggccgt
    28981 aacagaaggg tctcctgcat aatcatgacc cggaatggcg ccagcgctta atggggcata
    29041 tttggtaatg ttcatcagtt gcgtttcata tttttctgac gccagcgtta attcacgtgc
    29101 ttctgttact tcctgctgtg cgtttttcac ctgtgttgct aaacctgtgg ccctttgcag
    29161 agtaaagagc agtaagcagg aagtcaaaag caaaagctgc tttaagctca tctggtgcct
    29221 ccttaaactc aataatatta cagcattata gcttctgcat tatccaaggc aattagacat
    29281 tattatcaag cttgacagct aataacaggg ctcatttcct taccctaacc aattttatcc
    29341 tcccactact ggcacatcat ggtataatca tggtgagaat gaacttgtta tccatgggta
    29401 cacccgcaga atgactaacc ttgtcacgtc tcccatatat taatgttggt taagggaaag
    29461 ggtggtggat tgttaatgaa taaaagagac aaagctttat tcgtttgttt aattgtggtt
    29521 gtcctgctaa attggtggca aataaactca tcgcaaaagc tgatgaacgg cataagccag
    29581 atgcagagtg aaattaattc gctaaggttt caaataagta atgaaattgg tagtgtttct
    29641 ggtgttgttc agcaaataca ggacgaatcc cgctggtggg ctctggatga aactgagata
    29701 ttaaacgcgg gcagagaaga ggcagaagtt aaaattagct ggtatttaag agaatatcaa
    29761 gaagatagct tagtcacctt taattatcga ccggtaggag aaaatgactt taccaccgta
    29821 gctgctgtta aaggcagtga aggttacttt tattcaatcc tcaacttagc agtaatacca
    29881 gaacctatgt gggatatttc aattagtcac agtagtaact ccaaccaatc agatacattt
    29941 gaagaggttt atgacaaaga gcgtgttgaa ttaagtcaaa aaatgcgata tgaatactat
    30001 gtggctacca caaaggatgg agtaatccgt accggagaag ttatgcaatt agacttaaat
    30061 gagatgaggg attatctctt taattctctt catactcatg tcagtacaca acaaaacaga
    30121 cctacttata tttctttagt agagactaaa tatcttgaga atgtgcctta tcgtttggta
    30181 gaagctcatg tcgaaacccg catgggaaac agagttattg aaaaatggcc tctagaaaaa
    30241 caaggtgaca cgaatgaggc tgcagtcttc gaaagccgaa ttgaaacaac tcaggattac
    30301 gatgacctct atattgttgt gagatacagt gaagggttta ctgttgaaag gaaaatacag
    30361 tagattttgc tcgtgaacca ttgcttatgc tgaaaaacaa tattacacgc tggaattcaa
    30421 agatagctcc ttatactgtg gttaacatca cagcataagg agcttcgctg ttttgagcca
    30481 atagtatcac cgctgaaaag aaaatacagt aagcttcatt aaaagtagta tgattacaga
    30541 tcaaaactca aaaactaagt taaggaggcg gtcagatgca aaaatatgat acttttgctc
    30601 aatttaagga gattacgcaa aagaatagag cagagaaaga aaaagaactc ggctatacca
    30661 tgctagatac aaacaaagga agggatgtct gtattaggca aatagaaaga aagatagaac
    30721 aagttattga tgaatagcta gagtatctgt ggtgttggta tctggaaatg aagaaaattg
    30781 ttgagacgct aaaggaagca gattgcaaat agccaggacg caggtttcta agacctgctg
    30841 tcctggctat ttatgtcctg caccatgacg aatagaaagc cattgccagt gaaccggcca
    30901 ctactccacc ttctctctca ttaaattaga cacatgatca agagaaatgg tcgcattttt
    30961 tctgcaaaaa agcttaatgt cagtataagc tatctgcata tatttgtcag cggcacaatt
    31021 gaccttatgc cgtttgccct attctcctgt taccacagta tttatggcaa agtgtagaga
    31081 agctagctct ggcttaagcg tgtcatcttt tattatgccg atgtatttat aataatgaag
    31141 agcttaaaga cggagggatg aataatgaaa cttgcagaaa actatcggga acaattcctg
    31201 gcgtgggcaa aagataactt gttgttggaa agagaaagta gtacagagga actttatgcc
    31261 gccttgctta aggacaaacc tgtgcttgcg aaaagaatca acaaagccgc cccaaagact
    31321 ggtgccaaag cttatatcgg caggtatctg ctgcgagcat ttgtaaacca aggctggcta
    31381 aagtacaggg acaaaaagtg gtttgtgcag cctttagaag accattgtgc caactgttta
    31441 accgaacttc acccggatat ttatttagtg gatacagtgg gcagctatta ttgctgcgaa
    31501 gactgtttgt tggaaaggga agatggtgcc actgacggtt actggatgga ctatctgcca
    31561 ctctttgctg attttgcaga tctactgccg gaaagcagcg atcataaaca acaaaagaaa
    31621 gaagctgacc ccctgactca tctagaagtc attaacatca gaaaaagact ggcaaatgta
    31681 attaactgtc ctgattatga cacaatctgg ctaaacggtg gtgatgatgg tcctcttgcc
    31741 gcagaaatgt acaggatgct aaccatgctg gcagcaagac tgacaaaggt tacggaagtc
    31801 gaaacagcaa tcgccaagct gcgagcacca cagcaggtca actataccat tcaactacct
    31861 gaaggcctgc ccaatgaaaa acgcctacag gttaaggtag agcgtttcag caaacgccat
    31921 aagcagattc gccgtcaaga tttgcccctc gcctggcgga cggatgactt gagcctgcta
    31981 tctagatggg aaaagcagct gaaggagatt agttacacca tcgtcaaatc acgcaagtgc
    32041 ccactttgca gcatcgatgc cgacaatttt gcgctggctg tagatggtta tcgttattgt
    32101 gaagcttgct atgagtatta tgaattacaa agttatcaag tcgaggagga gtatgactgg
    32161 tgaaacgaga gtttatcgtc tattacgatc tagcggatgc agtctgcttt tatggtgaag
    32221 ctgtaaatgg aaaggcccag atattaccga aatggcagac tttactacga aaccggtgag
    32281 ctctggtatg agggtacatt taaagggcat cccttcaaaa gagtgttcca tgaaggcatt
    32341 gaatatcaca aggaaggcaa ggtaaaatgc cagcatcctg atttagacgc acaagattag
    32401 aagaagttgt tttaacagac aaacagaaac taaagtttga agatgacaat actattgaaa
    32461 aggaataaag aagaagcagg ccactactga tgactttaac tgcattagca atggcttgct
    32521 ttgtaaagga gatctaacct aacatagaga atatttacta cgtggaatgt tctggttgtt
    32581 tctcctgata acaggaacta aagcagcttc atcattgcta tactcagtaa gatagtaata
    32641 tctaggcaaa ggagtgagac ggtggcacaa acattcagtg tggaagttga agccgaggcc
    32701 agtgagctct atgcttatct ttgtcataaa atgagccatg tgcaaaaggt cagtgaagat
    32761 gttttttctg atacagaaca gtatgtagcg gtgctgatct atgaacaata cttctggcgt
    32821 gttggtaacc agatagccct aacggtaatc attaacaggt tagaatcagg gcgttgctgc
    32881 ttaaaaacaa tcagctgcgg ttccagcaag ggcatggtat ttaaattaga tctgggggca
    32941 gccagtgctt ttgcccgtga acccattaac tatgctgaag aaagatacaa cgtaatctgg
    33001 caacgctaaa aacatagcac agccagatgc cggtaaagat aagaagaaga aaagcagcag
    33061 ccaccttaga ataaagggaa tgctgctgct agtggagttg atgagatgaa ttttacaata
    33121 ggtgtctgtc agcttaatgt tgctgacaat aaggaattaa atctaaagaa tgcggaacgt
    33181 gcaattaggg aggccgctgg caaggggagc caactggtag ttttacccga gatgtttaat
    33241 tgtccttatg aaaacaaagc tttcccttta tatgcagaag agtatccggg ggagacaact
    33301 tcaatgcttg gtaagctggc caaggaatta aaaatttacc tggtgggagg gtctataccc
    33361 gagcgggatc acgacgccat ctacaatact tcttttatct atgataaaaa tggtgcttta
    33421 ataggtaaac atcgaaaaat gcacttattt gacgtagacg tagaagacgg tattagcttt
    33481 agggagtcgt ccagtttggg atctggtgag caagtcaccg ttgtagctac tgaattttgc
    33541 cggataggtg tggccatttg ttatgatatg cgctttccgg agctgatgcg cctgatggca
    33601 ctggcaggag cagaggtaat cattgtgccg gctgccttta atctgactac agggcctgtc
    33661 cattgggaga taaccgcaaa agccagggca ctggataatc aagtctactt cgtggcggca
    33721 tccccagctc gcaatctgca ggcagtctat catgcctatg gtcattcttg catcatagat
    33781 ccctggggtc aggttgttaa tgaactagat gaaaaagagg gcatcttata tggtgaaatt
    33841 gacctgcaat ttgtgcagaa agttagaaga gagctgccgc tactgcagca catcagaaag
    33901 gatatctacg aaatcaggaa aaaataagtg cctgctccca ttttaaatat tgttttctcc
    33961 ttgttctgtg gtgattatat taagtagatt ttctttagta ttcatctccg gctcttccag
    34021 cactttttcc agtaagctgt tcagtatctg ccccatctga accccgggtt gaataccaat
    34081 cgttattaag tcatctccat tgacagccag atcttttaca gtgagcggct gtttttcttt
    34141 gataatcttg cagacctgct cgcttaaagc attcagggca gagaagtgat gtggcggctg
    34201 ggaagctttt atctcagcaa tttgcagctc aaagaagctt tgtaaattcc ccaggccgac
    34261 tctattgata agccttttta tattggcctt attggggaaa tcaaaccggt tcagctgttc
    34321 tcgcaccagt atacatactg agtcgaccgt ctgcttagca aattttaatc ttctcagtat
    34381 atcccggacg atatctgccc tttctaaatg ataccgagac aagtccccgc tcttattctc
    34441 tttctttgat atcggtttgc ctatgccatg cagcagggcg gccagcctga ctgttaaacc
    34501 ctttggcgcc gcgtccaaga cggctaaaac atggtcaaag tcagcacagg cagtcagctc
    34561 tggcagaatc atacttaata gcaatgtaga gtgcagcagc tcaatacctc tagcgggttg
    34621 tttagaaagc ataattctgc ataattcatt cctgattctt tctgcactaa tatgccttaa
    34681 aagctgatgg tttctggtga tggccgttaa ggtactcgta ccgactgtaa aacctaactg
    34741 acaggctaaa cggatagcgc gcatcatgcg caatgcatct tcctggaagc gttgttcagg
    34801 atcacccacg gcattaatca cagcgcatct taagtcactt acgccgttaa aatagtcaac
    34861 taaccctgtc tgctgttgat aggccatggc gttaatcgta aaatccctgc ggctcaaatc
    34921 ctccttcagt gacgtagtgt tgtcagatct acgaacaccg gtaccattgc cagcatggcg
    34981 atatgtagta atatcatact gcttgccatt tataacagct gtcactgtgc cgtgttttat
    35041 acctgtagca atagttttgg caaataattg cttaacctga tcaggctgtg cattggtggt
    35101 aatatcccag tcatgcggta ccttatttaa cagactatct cgcacacagc cgcccacaat
    35161 atagcctgca aaaccatgtt catgcagctt atttaagatg tatgcaacat caccgggaag
    35221 actgatgttc atttgtactt taactcctca tacaatatat tgtgatgctc atcactattg
    35281 acagcggtcg gtccctaaaa gcaattcatc aaaaattact aaaactttag cgattgttca
    35341 ccagttcaag taagtgccac gaggtggacg gctgttaggt agccttctaa tggttttccc
    35401 tttaatgact attgcattcc aaaaggcatg ctgtttagtt ttacgatggt tagagaagtt
    35461 aatgatgatg gaaggtaggc ggtttcaatg aaaaagtttt ttctcatatt gttttctctc
    35521 ctacttgtgc tgctgttaac cggctgttca ccgaatcaga atagatcaga acccaaagga
    35581 gaagccatgt ttcagccttc ggtagctggt ttaaaagcca tcactttaga caccattgaa
    35641 gtaactgacc caagtctgat aagcctctta tctgctgagt tggcaaaagc agttccttca
    35701 gagcaagcac ccgataatgc acaccctgaa acgctgacac tcacagtaaa cggcagggaa
    35761 gaatcatatc aaatatacat accaggcctt gataccgccg caccagctgt actaaaaagt
    35821 gctgaaggct ggtatcaaat tccggcagaa tttattaatc ttttgtatag cttaactgaa
    35881 taccctaact atagtgctgc agtagatgct aaagataaag agctgctggc tgaatatgga
    35941 ctgacaccgg catttctgat taatcaactg caggtaaaac tgccaactga tcttctgcat
    36001 cgggccggtg aataccctat aatactgtat tgggcacata ataatgaatt aagccaagcc
    36061 attaatctta atcttgaaaa agccttgggg caacaggtgg atgttagatt atacaagatt
    36121 gtggagctac tccccgacta tctgagtccc cgctgtcata ccgggcgtgc tgttatcgtc
    36181 caccatgctg ataaaattat tggtgcctgg ctggatgcag gccggcatta cggttttgcc
    36241 tgttcacttg acggcaaaac actcgaagaa gttacaaaca aaagttggga ccaatggatt
    36301 gtctcccaca tcgactatga tgaccctttg gaaaaagagc ttgcgctaaa gtcaccggaa
    36361 gatattatcc agacttacta tgcagccatc aacaataaag atgataaacg agcttatgcc
    36421 tgcttatcac gtgaaaatct ttcttcctat cttttcagca acctggataa ctactatcta
    36481 atgaacccag agtacgaagc atatgatggt tttgccaata taacctctgc ctcggtcatt
    36541 aagatagaac catactcgct caacagtgaa caatctgagg agctgctttt ctatcaagta
    36601 acggtagatt tagaggtaaa agagccaatg acttttgaga gcggcagcca aacccgcttt
    36661 atccacttga agcaagaatc accgcaaaca ggttggcgca tttcatcgat aggaaccggg
    36721 ccctgatgta tacaaagatt atgtttacgc atcaacacgg aaggcaaata agtctcgttt
    36781 ccagaaacct gcctggtgag caagtaatgc gattcatcac cgtgctgacg aacaaagaca
    36841 cctttacgta ctgtataaag gtgtctttgt ttaaatctcg aacccagaac cctatttgaa
    36901 taaattaggg gtcgatgaac attaaaaaaa aatagttcaa gcaggcattt ctaatggtga
    36961 agaccactct ataaaactca taaaaaaatg ggcaaaaaga atccggagcc tgcgttccag
    37021 attcattact aataactgca tggcaataac cgtctcacta gtacctctga gacgtgccat
    37081 aatctg
//
LOCUS       DASRMS010000040         5623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_210722, whole genome shotgun sequence.
ACCESSION   DASRMS010000040 DASRMS010000000
VERSION     DASRMS010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210722"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1456)
                     /locus_tag="VFC74_01940"
     CDS             complement(<1..1456)
                     /locus_tag="VFC74_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24129.1"
                     /translation="MLVGEVFARMGLDSKQYEKGLGKLEGMTQKKAVTLGSIFKGAFS
                     FALGMGLVSGFKSLGGALTDFVNTAARTEVLDVALKSVAKSSGYAISTLRQQRNAVME
                     LGIAEQEASQILTRFMQAQLDTADAAKLARVAQDAAVISGTNSSQAAEQMTEAIAKQR
                     PMLLAQFGMMAGIEEMYGEYAKTVGKASNQLTQAEKKQAMLNYILAEGDKIAGTYEAS
                     MGVVGKQISSLPRYWDTLKNAIAAPLALPAISVIVDGLTNGLKNAIGWAEANKMMLQS
                     MGQTAANVAGYVVSGFQFVTRTFAENWGMIRFAATALLTYAVATRTAAGATALFQAIS
                     LALNGQLVAKIPLLGFVSTAMGIYRVQMALASSQGIVLSGALAKVRLALFSVWSALGP
                     IGWAILALSGLVAGGMALWNRYSQSLQKTASTGMGDFRKAEQGLAASAGDSSKALDDQ
                     TKALNKAGKAAQKNLQPFDEINQMQKESAGGSGAG"
     gene            complement(1450..1761)
                     /locus_tag="VFC74_01945"
     CDS             complement(1450..1761)
                     /locus_tag="VFC74_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24130.1"
                     /translation="MRQLTVRELSLILDELQRQVALEQYNEQRNRWASLAAVITNGCG
                     AIAGMFSKKKHKEVSPDDFISKDAKKAFQQLLGQDKQKDWSKHIEDAKAKGLKGGETL
                     C"
     gene            complement(1803..2141)
                     /locus_tag="VFC74_01950"
     CDS             complement(1803..2141)
                     /locus_tag="VFC74_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24131.1"
                     /translation="MRNKTVEFAGKKIRVEEKRIGELEKIVADLFPESKGNIQKVDLN
                     KVLEKAGFDLLYDKLPVLFSDITKDDVKNAYMSEIEELIEVFIDVNFTGLKRLINPLM
                     GLIQAGLTQK"
     gene            complement(2152..2766)
                     /locus_tag="VFC74_01955"
     CDS             complement(2152..2766)
                     /locus_tag="VFC74_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24132.1"
                     /translation="MAITGMVGGVYVSDNSIPPVPFFDATTTGDAARKRYQVVDPAHR
                     YWPLDAEVTVKVNAVEVTTGFRLERAGGFVEFDDALGAEDVVTVSGTALTLAQAGGFF
                     NWSIDGDGEDADATTFASQGWKESVRTLKGWSGSAEAYWGDSQFYDSLGKAIIVKLFT
                     DAGPSQNCLEGFAIINGDGIETPVDGLVQEAVDFTGTGPLYIRL"
     gene            complement(2780..3172)
                     /locus_tag="VFC74_01960"
     CDS             complement(2780..3172)
                     /locus_tag="VFC74_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HZK24133.1"
                     /translation="MISVALAGKGGSLNIGANKVAEIANWSLDLGADDIDVTSFDSEG
                     WKEYLAGLKEWSGSAEGNFKPDDTNGQKAILQAWLNGEALNFSFKVSETITFTGSAFV
                     KPSIEMPVDDKASFSTDITGTGPLTLPA"
     gene            complement(3165..3524)
                     /locus_tag="VFC74_01965"
     CDS             complement(3165..3524)
                     /locus_tag="VFC74_01965"
                     /inference="COORDINATES: protein motif:HMM:NF024103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HZK24134.1"
                     /translation="MLTEISTYLKNQDTDIFLGLMPDQPDNCVALFEYAGSPPDLHWN
                     GEYPGLQVRVRDKSYAAGRAKIGHIMRVLHGLTETVLGSTRYLLIRARGTPEILKRDA
                     SNRVEFFVNFEIIKERD"
     gene            complement(3532..3957)
                     /locus_tag="VFC74_01970"
     CDS             complement(3532..3957)
                     /locus_tag="VFC74_01970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24135.1"
                     /translation="MAKKWRIKEAVKIAEKAGLKALRTGGEKIITSAMNNVPIESGTL
                     RRSATTTVGGLPNSSEVYESAKDGAEMKNAYPEPLGKEKAVYVSYNTPYARRQHEELG
                     YYHPVGGGPKYLENSFNQNKSKVLKDADKQIKKALREAE"
     gene            complement(3957..4250)
                     /locus_tag="VFC74_01975"
     CDS             complement(3957..4250)
                     /locus_tag="VFC74_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24136.1"
                     /translation="MISGYLNQTATWHYVTGQNEYGEPITGSKAVKVRWEGKRRLVRD
                     NEGREVVSEARVFCTEAVKPGDELEFDGRRWPVIAVSTIPGLDGNESHREVAV"
     gene            complement(4247..4702)
                     /locus_tag="VFC74_01980"
     CDS             complement(4247..4702)
                     /locus_tag="VFC74_01980"
                     /inference="COORDINATES: protein motif:HMM:NF042800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaT-like ssDNA-binding protein"
                     /protein_id="HZK24137.1"
                     /translation="MPEEFETTITVGENSYIDIDGADEYFAGRLHAESWGQADDETKE
                     KALKQATKEIDRQLLKGRKATDTQELAFPRYPDTEVPVAVKDACCEIALALLERGNSQ
                     RRKLQQEGVQSFTLGNMSETYTAGAGRGLISQEAKELLKLWLLGGVFIT"
     gene            complement(4837..5379)
                     /locus_tag="VFC74_01985"
     CDS             complement(4837..5379)
                     /locus_tag="VFC74_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24138.1"
                     /translation="MRFLDYLKRFTYTPNEFYDHLKGLEDAAGGDVDLVILPVMAGES
                     SNQPALKPTVSEANSTENPYVATVAVKLMNKANDKVLEWFNGTRKVTVDISSTAGTIA
                     IDDEDAGSAGADATFDLFFENGVGTFIITLGGAWEAEDTIKVNADKDDSNYSVGIMGY
                     TVKVENHFLIEVAADSQQEE"
     gene            complement(5391..>5623)
                     /locus_tag="VFC74_01990"
     CDS             complement(5391..>5623)
                     /locus_tag="VFC74_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007780449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24139.1"
                     /translation="ALSGDDILVNRRHFILHPRGVAFRNAQLGDGNDGTNATPSNANL
                     ADHRNWIRVYEAKNVRIVQFKHKLVTAYSAAQ"
BASE COUNT         1266 a         1554 c         1165 g         1638 t
ORIGIN      
        1 atccggcgcc actgccacca gctgattcct tctgcatctg gttaatttcg tcgaatggtt
       61 gtaagttctt ctgtgccgct ttgccagcct tgttgagtgc tttagtctga tcatccaggg
      121 cttttgagga atctcctgca ctggccgcta gtccctgttc tgccttacga aaatcaccca
      181 ttccagtgga ggctgttttt tgtagagact gggaatatct attccagagc gccatgccgc
      241 cagcgaccag accggatagg gccaaaattg cccaaccaat tggacctaat gctgaccata
      301 ctgaaaacaa agccaatcga acctttgcta aagctccgga tagtacaatg ccctgcgagg
      361 atgctaatgc catttgcacc ctatatatac ccatagcagt gcttacaaaa cctaatagcg
      421 gtatctttgc taccagttgc ccatttaggg ctagtgatat tgcctggaac agcgcagtag
      481 ctcctgctgc tgtcctagtg gccactgcat atgttagtag tgctgtagca gcaaagcgaa
      541 tcatgcccca attctctgcg aatgttctag tgacaaactg gaatccactt actacgtaac
      601 ctgctacatt cgcagcagtt tgccccatgc tttgcaacat cattttatta gcctctgccc
      661 agccgatggc attcttaagc ccattggtaa ggccatccac aataacgctg atagccggca
      721 aggctagagg tgctgcaatg gcgtttttca gtgtgtccca gtaacgtggt aaggagctga
      781 tctgcttacc aaccacaccc atactagctt cataggtgcc agcaatctta tcgccttcgg
      841 ccaggatata attcagcata gcctgtttct tctcggcctg ggttagctga tttgatgctt
      901 tccctacggt ttttgcatat tcgccgtaca tttcctcaat accagccatc atgccgaatt
      961 gtgccaataa catcggacgt tgcttggcta tagcttctgt catttgttcc gctgcctggg
     1021 aggagttcgt gccactaata actgcagcgt cctgtgctac cctggcaagt ttggcagcat
     1081 cagccgtgtc taactgcgcc tgcataaacc gggtaaggat ttgcgaggct tcttgttcgg
     1141 caataccaag ctccataacg gcgtttcgtt gctggcgtaa ggtcgatata gcatagccgg
     1201 aggatttagc tactgatttc agggctacgt ctaatacttc cgtccgggca gcagtgttta
     1261 caaagtcagt caaggcacca cccagcgact taaagcctga tacaagcccc atccctaacg
     1321 caaaagaaaa agcaccttta aagatgcttc ctagcgttac agctttcttc tgggtcatac
     1381 cttctagctt acccaggccc ttctcgtatt gctttgaatc cagtcccata cgggcaaata
     1441 cttcaccaac tagcataatg tctcaccacc cttcagccct ttagcctttg cgtcctctat
     1501 gtgcttgctc caatcctttt gcttgtcctg tcctagcagc tgctgaaatg ccttttttgc
     1561 gtctttgctt ataaagtcgt cagggctaac ttctttgtgc tttttcttgc taaacatacc
     1621 ggctatagcc ccacatccat ttgttatcac cgccgccaag gacgcccagc ggttacgctg
     1681 ctcgttgtac tgctctagag cgacttgccg ctgcagctca tcgaggatta aggacaactc
     1741 tctaaccgtt agctgtctca tctcatctat cgtccagcca aattcacgag caaaaagtac
     1801 tactatttct gcgtcaagcc agcctgaatc aagcccatca gcggattgat tagccttttt
     1861 agtccggtaa aatttacgtc aatgaatacc tcaatcagtt cctcaatctc gctcatataa
     1921 gcgtttttaa catcgtcttt tgttatgtct gaaaaaagta ctggtagctt atcatagagc
     1981 aggtcaaatc cggctttttc tagtaccttg ttcagatcta ccttctggat attgccctta
     2041 ctctccggga acaggtcagc aacaatcttt tcaagttcgc caattcgttt ttcttctacc
     2101 cggatctttt tgccggcaaa ttctactgtt ttatttctca taacaatccc cctacaatct
     2161 tatatacaac ggcccagtgc cggtaaagtc aactgcttcc tgtactagtc catctactgg
     2221 cgtttcaatt ccatcaccgt tgataatagc aaatccctca aggcagttct gtgacgggcc
     2281 tgcgtcagta aatagcttta cgatgatggc cttacccaag gagtcataaa actgcgaatc
     2341 gccccagtaa gcttctgcac tgccagacca gcctttgagt gtccgcacag actccttcca
     2401 tccctggctt gcaaatgtcg tagcgtcagc atcttctccg tcaccatcta ttgaccaatt
     2461 gaagaagccc ccggcctggg caagggttag ggctgtacct gatactgtaa caacatcctc
     2521 tgcaccaagc gcatcatcaa actccacaaa gccgccagca cgttccaacc taaagccggt
     2581 cgttacctca acagcgttta ccttgaccgt aacttctgca tctagtggcc agtatctgtg
     2641 agcaggatcg acaacctgat aacgctttcg tgctgcatct cccgtggtcg tagcgtcgaa
     2701 aaaaggaacc ggagggatgc tattgtcact tacatagacc cctccgacca ttccagttat
     2761 agccatttat tatcactcct tatgccggca acgtcagagg accggttcca gtaatgtcag
     2821 tactgaacga cgctttatcg tcaaccggca tttcaattga cggcttcaca aaagctgacc
     2881 cagtaaaggt tatagtttcg cttaccttaa agctaaagtt cagtgcttct ccatttagcc
     2941 aggcttgtaa aattgccttc tgtccattcg tatcgtcagg cttgaagtta ccttctgcgc
     3001 tgccagacca ttcctttaaa ccggctagat actctttcca gccttcacta tcaaagctgg
     3061 taacatcaat atcatctgcg cctaagtcca aagaccagtt agcaatctcg gctaccttgt
     3121 tagcgccaat gtttaagcta ccgcctttgc ctgctaatgc cacgctaatc acgctccttt
     3181 ataatctcaa agtttacaaa aaactcaacc cggttgcttg catcacgctt taatatttct
     3241 ggggtgcccc gggctcttat taataaataa cgagtgctgc caaggactgt ttcagtcagc
     3301 ccatgcaaca ctcgcattat atgtccgatc tttgctctgc cagcggcata gctcttatca
     3361 cgcactctta cctgtaggcc gggatattca ccattccagt gcaaatctgg agggctgccg
     3421 gcatactcaa acaatgctac acaattgtca ggctggtcag gcatcaggcc aaggaaaata
     3481 tcagtgtctt gattttttag atatgtgctt atttcagtta gcacaacggc atcactccgc
     3541 ttctctcaag gcttttttta tctgcttgtc agcatccttt agcactttgc ttttgttttg
     3601 gttaaacgag ttttctaggt atttaggccc tccccctaca gggtgataat agcccagttc
     3661 ttcatgttga cgtcttgcgt atggcgtgtt gtaagataca tacactgcct tttctttgcc
     3721 taaaggctcc ggataagcgt ttttcatttc tgccccatcc ttcgcagatt catacacttc
     3781 tgaactgtta ggcagtccac caactgtagt agttgcgctt ctgcgaagag taccgctttc
     3841 gataggcacg ttgttcatcg cactggtaat aattttttcg ccgccggtcc gcaatgcttt
     3901 aagcccagcc ttctccgcta tcttaactgc ttctttaata cgccattttt tagccattac
     3961 acagctacct ctctgtgact ttcgttgccg tccaagccgg ggatagtaga taccgcaata
     4021 accggccacc tacggccatc aaactctagt tcgtctcctg gctttacggc ttcagtacaa
     4081 aatacccgag cctctgatac tacctcccgg ccttcattat cccggactag ccgccgcttg
     4141 ccttcccagc ggactttaac agctttactg ccggttatgg gctctccgta ttcgttttga
     4201 ccagttacat agtgccaggt agcagtctga ttgagatagc cactgatcat gttataaaaa
     4261 caccacctag cagccacagt ttaagcagct ccttcgcttc ctgactgatt agaccacgcc
     4321 cagcacctgc agtataagtt tcgctcatat tgccaagcgt aaatgactgt acaccttcct
     4381 gctgcagttt cctccgttgg ctgttgccac gctcaaggag agctaaagca atttcacagc
     4441 aagcatcctt gaccgctaca ggcacctccg tgtccgggta gcggggaaag gccagctcct
     4501 gggtatccgt tgccttgcgc cccttgagaa gctgccggtc tatttctttt gttgcttgct
     4561 tgagggcttt ttctttggtt tcgtcgtctg cttgccccca gctttcggcg tggaggcggc
     4621 ctgcgaagta ctcgtctgct ccgtcgatgt cgatgtagct gttttctcca actgtgattg
     4681 tggtttcaaa ttcttctggc atttagcctc cgcctccttc ctctcccttt cagcctgcgc
     4741 tttctcttcc tgctccctgc gcatgcggtt aaaggctgtc acatctgttg ccataaaatc
     4801 acctcaaaat agaaagaggc ccgaaggcct ctcgttttat tcctcttgtt gcgaatcagc
     4861 agccacttcg attaagaagt gattttccac cttgacggtg tagcccatta ttcctacgct
     4921 gtaattactg tcgtctttat ctgcgtttac ttttattgtg tcttctgcct cccaggcgcc
     4981 gccaagggtg attatgaacg ttccgacacc gttttcaaag aataagtcaa aggttgcgtc
     5041 tgcgcctgcg ctgccagcat cctcgtcatc aatggcaatt gtgccggctg tggaactaat
     5101 gtccaccgtc accttgcgag tgccgttaaa ccattcaagg accttatcat ttgccttgtt
     5161 catcagcttg acagcgacag tcgcaacata gggattttct gtactgtttg cttcgctcac
     5221 cgttggtttt agtgccggtt gattagaact ttccccggcc atgaccggga ggatgacgag
     5281 gtcaacgtca cccccggctg catcctctaa ccccttcaag tgatcgtaaa actcgttcgg
     5341 ggtgtaagtg aaacgcttta gataatctaa aaatctcatc tcatcacccc ttattgagct
     5401 gcagaataag cagttacaag tttgtgctta aactgtacga tacgcacgtt ctttgcctcg
     5461 tagacgcgta tccagttccg gtgatcggct agattagcgt ttgacggtgt cgcatttgtt
     5521 ccgtcgttgc catcgcctaa ctgagcgttc cggaatgcca ctcccctcgg atggaggatg
     5581 aaatgtctgc ggtttacaag gatgtcatct ccggacagtg cat
//
LOCUS       DASRMS010000041        46733 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_948811, whole genome shotgun sequence.
ACCESSION   DASRMS010000041 DASRMS010000000
VERSION     DASRMS010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 46733)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46733)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46733
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948811"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            731..3205
                     /gene="leuS"
                     /locus_tag="VFC74_01995"
     CDS             731..3205
                     /gene="leuS"
                     /locus_tag="VFC74_01995"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HZK24140.1"
                     /translation="MVAEYNPSVIEPKWQRFWEENDLNQTEEDRQKKYYVLEMFPYPS
                     GRLHMGHMRVYSIGDVLARFLRMRGFSVLHPMGWDAFGLPAENAAIKNQVHPAAWTYA
                     NIEAMKKQQKALGISYDWQREVATCDPDYYKWTQWLFLLLYKRGLAYKKQAGVNWCPN
                     CSTVLANEQVEDGGCWRCGTEVTHKDLEQWFFRITDYTERLLEDLKLLSGWPERVRMM
                     QENWIGKSEGAEVRFKVKGREDEISVFTTRPDTLYGVTYLVLAAEHSLVEALTAGSER
                     QAEVQAFVENTKKLNDIARASTEMVKEGVFTGAYCLNPVNGEEVPILVGNYVLMGYGT
                     GAVMGVPAHDQRDFEFAKKYQLPLRVVITPEDQELSAADLKEAYVDAGVLINSAQFNG
                     KTSEAAQKKIAAYLAANNWGEAKVTYRLRDWLISRQRYWGAPIPVLYCEHCGTVPVPE
                     EQLPVLLPEDVVFKAGGQSPLAEHEGFLHATCPQCGGQARRETDTMDTFIDSSWYYFR
                     YTDAQNENLPFAKGQGDAWVPVDQYIGGIEHAILHLLYSRFITKVFHDAGYTAAVEPF
                     TRLLAQGMVNKDGAKMSKSKGNVVSPDEIIEKYGADTGRLFILFAAPPEKDVDWSDRG
                     VEGSYRFLKRVWRLVDRYADVLQQPVEQQVAADDADQALRRAMHTALKKVTEDIEERF
                     NFNTAISSIMEAVNSAYSYMHEKEDHIHHGMMREVLEILVVMLAPFAPHLGEELWERL
                     GHQTSVHLQSWPTVDSTALQVAEVEIVVQVNGKVRDHLVVPTGLTREALQELALASAK
                     VAQLAADKQVVKVIAVPDKLVNIVVR"
     gene            3505..4074
                     /locus_tag="VFC74_02000"
     CDS             3505..4074
                     /locus_tag="VFC74_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HZK24141.1"
                     /translation="MFQMTRREQLGAIILVVLLLLGLLLRYLLLPKPVALEVLPPPET
                     PLDEEQPQAEIVVHIAGAVRTPGVYTLPAGARVYMALEAAGGVLPEADEHALNLAEPL
                     FDGRRITVPFQGEQGAAAAASDNDKVNINTAQAAEFEQLPGIGPAKAAAIIAYREENG
                     PFQSVDDLTRVSGIGINTLNVIKDYLTLY"
     gene            complement(4071..4307)
                     /locus_tag="VFC74_02005"
     CDS             complement(4071..4307)
                     /locus_tag="VFC74_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24142.1"
                     /translation="MSSWRWQALFILLVLGLLAIALFETPRLILNKMWRELLVFLVLW
                     LLASTLAIMQFYGIKIPAPRELLQKLLAVLKLKT"
     gene            complement(4300..5571)
                     /locus_tag="VFC74_02010"
     CDS             complement(4300..5571)
                     /locus_tag="VFC74_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ger(x)C family spore germination protein"
                     /protein_id="HZK24143.1"
                     /translation="MKKQLTILCLLLFCLSLFTAGCWDQIEVEELALVRAIAIDYQPG
                     RRSPYLVTFAISLPSDVAGGEGGGGGGGEPTQLFSGVGASMDLALHQASLNLSRQLFL
                     SHASVILIGEEMAKHGLAHVFDFIIRNPQVRLNAYLLYTNGFAQQMLTTTERLEIGIT
                     EEIIGMIEQARFISETEPLPAYKFLQKVVTPGQAAYTALLQKGSPLEEIIPGLQGEQK
                     PGGQEESSGGSDSSEGGGGEKAKEVLTLAGLAAFRNQKLAGLMNAQETRGFLWFLGKT
                     SRTYLAISDPQKPKEIITLHVSRAETKIKPQIKNGQLSFSVEVVTEGDIRSQTSTTKI
                     NTPEMMKKVNSANAAVIKQEMEKALHRMQQLQTDLVGFGSILNRHEPKLFSRLQDNWP
                     EEFSKLKVEIVVTSDVRRAGMQANPALPRGE"
     gene            complement(5568..6698)
                     /locus_tag="VFC74_02015"
     CDS             complement(5568..6698)
                     /locus_tag="VFC74_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endospore germination permease"
                     /protein_id="HZK24144.1"
                     /translation="MKKAKIGSLEKGKISPRQGGELLVISLLATSILLVPAVAVEQQG
                     QNAWLAVLLGTIWGLVTLSIVCWLGNKHPEETIFQYTETLLGKWLGKVVGLLYVWSFL
                     RVVAIVIREFGDFMTTSFMPNTPLSVFSLSLIFLAAWTVFAGLEVLARMNEFIILLVI
                     GSLYFILLLSLGNWELGYLQPLFRVEFLSLLKTSLIPEVWAVDTITFAVIIPFLTRPQ
                     RALAAGAAAAVFANGLLAIGLISLLATFGPYFLLVTRFPILMFVRSISVAQILSRFEV
                     LIMVVWVAGVFAKTTFYLYCACLGLAQITGLKEIRPVVLALGTIAVVWSFSLFENITE
                     LEASVSTTSPRVLLMYFGIPLFLLVLTWLKEKLHKRQLGRMQ"
     gene            complement(6661..8319)
                     /locus_tag="VFC74_02020"
     CDS             complement(6661..8319)
                     /locus_tag="VFC74_02020"
                     /inference="COORDINATES: protein motif:HMM:NF015292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HZK24145.1"
                     /translation="MANAFTFLKKLVARKEKPKPPMTNNKQAARKPENDDTPVLSERL
                     ALIKKRLGESNDVVIREFNTSKDQVKLALLLIDGLVDKKMINDNIMLPILSNTLANEY
                     SEKLTASNAFELLNKHITAVSELSQASNLTQALKMVLSGDTALFIDGEQRIIVFGTRM
                     WEKRTVAEPVTEAAVRGPREGFTETMRINTSLLRRRIKHENLRFETMKAGRRTATDIN
                     LVYINDIVNQETLAEVRQRIAAIDTDAILESGYIEEYIEDAPFSIFPQLEHTERVDKA
                     AAAILEGRVLVMTDNTPFMLIVPTSFFQFIQASEDFYERPYVALFLRLLRLFVLNIAL
                     ILPATYVAVISFHQEMLPTSLLLNITAAREGIPFPAVAEALLMEGTFEVLREAGIRLP
                     KTVGQAVSIVGGLVIGDAAIRAGLVSPGMVIIVAATAISTFAIPAFNASVALRLLRFP
                     LIILGAMMGLYGVIFGLIIILIHLAGLQSFGVPYLTPLVHGTFRDMVKTIVRPPWWAN
                     IFRPRLLDPQQRRRQATNTWARHTPLEERELTHEESKDRQPGKG"
     gene            8467..11187
                     /locus_tag="VFC74_02025"
     CDS             8467..11187
                     /locus_tag="VFC74_02025"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HZK24146.1"
                     /translation="MKRIFPLLIIFVVLMLAWLAAGSYLDWLWFSHLGFASVYQTVLT
                     SSWAVGLGSFLFFAIFLFCNLLYVQKSILGMPNLVLRHLLMNTRFGDLLTPQRLRSVF
                     LGLALPLAWLTTVPFRNAWLTVRLFTAGANTGVTEPIFNADVGFYLFRLPLWELIYQY
                     LLLIFIVTLLVCGVLYLFIRPPQQLGLRTLFATRGQFHLSRLLAGTLLVRAGGYRLQM
                     YRLVYAPDGVVFGAGYTDVHALLPAYWVMLLLAVVGAVILILNTKLKQSRLLTGSIVS
                     IILASLVLNNLVPLAVQKFLVEPNEFVREEPYLQYNIDFTQQAYGLKKIEEREFPLNE
                     ALQYEELLANETTLDNIRLWDWRPLGQTFNQLQSLRQYYTFHDVDTDRYLINGEQRQV
                     MLAAREMVTSQLAAPTWINMHLAYTHGYGAVASPVNEATGQRLPKLFLHDLPIKGESD
                     LQVDVPQIYYGELTDNYIFTNTKTKEFDYPMGESNAVTSYDGEGGIPLGGPLRRLLFG
                     LRFADYRILVSNELTAASRLHFKRNLAERLHELMPFLQYDEDPYLVVADGRLFWMIDA
                     YTVSNRYPYAQPYSNINYIRNPVKVTVDAYHGTVHFYVFDADEPLLAAYSKIFPTAFT
                     PAAEMPPALAEHVRYPETLFKLQSEVYATYHMDNSNVFYNKEDRWQVPQEKYGNEPIE
                     VEPYYTILRLPAEAEAEFILMRPYTMYERDNMVAWLAARSDGEGYGQLVVYQFPKGEL
                     VYGPAQIEARIDQDTTISQQLALWNQQGSRVGRGNLLILPVNGTILYVEPIYLQAEQS
                     ELPELARVIVAYGDRVVMENTLEEALAAVFGLQLGDSEPDLPEKPPEPPETGGLPLPA
                     ASLETLIRQAAELYQEAEMALKAGSWAEYGRLQEELGRVLESLQEFSLQD"
     gene            11242..11676
                     /gene="rnhA"
                     /locus_tag="VFC74_02030"
     CDS             11242..11676
                     /gene="rnhA"
                     /locus_tag="VFC74_02030"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HZK24147.1"
                     /translation="MKQIVIYTDGACSGNPGPGGYGAVLLYGEHQKEVSGGEAKTTNQ
                     RMELQAAAAALEQLKEPCQVKLHSDSAYLINAFNQHWFVKWQQNGWLNSKKEPVQNRD
                     LWERLLALAEQHKIEWVKVKGHADDELNNRCDQLAREAIPRA"
     gene            11826..12698
                     /locus_tag="VFC74_02035"
     CDS             11826..12698
                     /locus_tag="VFC74_02035"
                     /inference="COORDINATES: protein motif:HMM:NF025616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HZK24148.1"
                     /translation="MTKKQFLGFLVVILLILAGCSAGRGTSDNAVGEGGMAGAPEENF
                     GKEAQERADADGSLGEAEQKLIRTASLLLDVEVLDDAIETVSVIVKGVDGFIAKSNVY
                     GAAENRSAELTLRLPAQQLDAVLDEIKTMGTVRRSSTGSNDVTLQYVDLEARIRNLSR
                     QEERLLDVLAQASTVEDILRVEQELARIRGELEAHTAEFRYLKDRVDYATADVFLYET
                     STASSTITTTGLTGVWQRGSTGFIKSINAMLTGLGNLLVFLFTSLPYLSIFALIGVPI
                     FLLVRKFMANRSRS"
     gene            12724..15063
                     /locus_tag="VFC74_02040"
     CDS             12724..15063
                     /locus_tag="VFC74_02040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HZK24149.1"
                     /translation="MVTFLPSLVLTFAAGIALAKQCPLSLWSWICLGTAALAAAIVLT
                     LKKKHAAAVLLLLFLGLGILVYQHAHRALGQPLAPLWGAEAEYQGYIQEVLSCSEEQA
                     QFNFYLEEAVLPEQKQLQVGALIRVSVYRPAASFQPFYGLPLKLRGTIKAASGQRNPG
                     GFDYANFLETENVSGLMSAAPWELTVLAGEKGNPVLVFFARLRAKAIILLQHYLPEEE
                     AQLAAALILGQKKELSETLLTAYQRLGLAHLLAVSGLHVGFVTAFALFVFTRLFSSRW
                     PLLSSLLAALFILAYVFLTGGQPPVWRAALTVLITYLARYSGRESEGLQTLSLVALLL
                     LFFRPLWLFSLAFQLSFAATGSILLLAPRLQGAFRWCPKFMASSLAVSIAALAGVLPL
                     QAAHFGYFSFYTIPLNLLCVPLVGLVVMLGVAGIVSGLVSSALASIFFTMTWPLLALL
                     ARLPRFSMRLPAAFLYLPAVHPLWWCIYAAVLLLIIANKSWSLTARRILFVLLLGNLA
                     CLSSFTGSRYRQLEVTLLDVGQGLAVHICTPAGQHLLVDAGGGQFATGEQIVLPYLRA
                     KRVPKLAALVLTHPHDDHYGGMQAVLAALPTNVYLSNGEQEDSASFSDLLLTLQEMAI
                     PQYRVSAGYRLLIDGVELKILSPPAKRFLNTSDDANNNSLVISLSYGDFNLLLTGDAE
                     AAALTELLQTAKGKLQAAVLQVPHHGSRNALSPALLREAAAPVAVIAVGNNPFGHPHA
                     ETLALLAEQKIDVYRTDLHGAVTITSDGKKWQITPYLYP"
     gene            15146..16390
                     /locus_tag="VFC74_02045"
     CDS             15146..16390
                     /locus_tag="VFC74_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK24150.1"
                     /translation="MRVLMFSWEYPPRVVGGLARHVAELSVALQAEGTQVDVITIDED
                     GTAPEVEIIQGVHVWRVRPYPLVPRDFISSVLQYNLAMLEKTLALLMQGEDYDLIHAH
                     DWLAAYAARAVKHALKKPLVATIHATEYGRNRGLHTEEQRYISDVEWWLTYEAWQVIC
                     CSQYMHAELQRVFHLPDDKIVPIPNGVNPRQFQTDALEAAARARYAAADEKIVFFIGR
                     LVQEKGVQILLEAVPHILAVVPKTKFVIAGKGPAWDDLHLQARKMGIEQHLYFTGYID
                     DVTRNKLYKAAATAVFPSLYEPFGIVALEAMAASVPVVVSDTGGLADIVEHGVTGLKC
                     YPGHALSLAEQIITLLQKPSFAQQLAAKAMQKVESKYAWQEIARQTRNIYQKVLADYQ
                     QSDWPTQQLLTQPKTVLPQCAQ"
     gene            16423..18912
                     /locus_tag="VFC74_02050"
     CDS             16423..18912
                     /locus_tag="VFC74_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HZK24151.1"
                     /translation="MKTIIMAGGQGSRLRPLTCDRPKPMVPLINRPMMEHIVALLKRY
                     GLRKIGVTLQYLPQQIKNYFTDGREFGVAMRYFTENSPLGTAGSVKNAASFLDETFMV
                     ISGDALTDFDLQQAINFHRQKGSLATLVLTAVEQPLEYGVVITTEEGQITQFLEKPSW
                     GEVFSDTVNTGIYILEPEILAFIPDTVKFDFSQDLFPLLMAKGYPLYGYVAKGYWCDI
                     GNLQQYHQAHLDILQGKVNIELQAREISPGIYVGEDVIIEPGAYLTAPVLIGNGACIK
                     QGAVVAESSVIGPQTQVDSFASVKRGITWRNTYLAPQTEIRGAILGNRVQVLQGTSLF
                     EGAVVGDDTVLEEHCTIKPNIKIWPHKRIASGSTVSESIIWGTKPTRNLFGSAGISGE
                     VNLEITPELVAKLAAAYGSLLGEGRQVVISSDDWKASRMLKAAALAGLLSAGVQVYDL
                     GVSVTPITRQAIKQLEVQGGIHVQLSYDVNTNTRLQFFDAAGLALTKAWERKIEQTYY
                     REDFKRIKGLAVGESNQLAQYADSYRENLLQNLDITVFARKRRKVLLAYVTPWLYTYL
                     LPILQQLNCEVISISAPEDGPLTLQALQEQFSSVAATVQRLNADLGVVMDANAEKLVL
                     FTPQGQIIAEELFRALITTIIFRSASGSTMAVPVTAPAVIEKLAAMYQGKVLRTKTTP
                     RSLMEALAAGDNCSQRYDPFTLSFDAISSLVKILEYLAAEATDLTTLVDSIPPFYVHQ
                     REVDCSWGAKGAVMRRLIEETRHADVELLDGVKVQHQQGWALVLPDSEKPVYRIYSEG
                     YNAEIAEELTDLYAQRIKKYQTELGKQRNES"
     gene            19022..19474
                     /locus_tag="VFC74_02055"
     CDS             19022..19474
                     /locus_tag="VFC74_02055"
                     /inference="COORDINATES: protein motif:HMM:NF042610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6789 family protein"
                     /protein_id="HZK24152.1"
                     /translation="MDKVVSGGIAGGIAGLLVGLLSKILSKVKLCQLCLIKIGGGLSN
                     QAMLAENSALLWQVIGWLSHLLISMVLGIIFLYLLHYTGKEYILLKGVMFGAVVWLVD
                     ITIISPLLGFIPARSRPLDLLILLAYHIIFGYLAAVLSRRFAKTTAQA"
     gene            complement(19522..21144)
                     /locus_tag="VFC74_02060"
     CDS             complement(19522..21144)
                     /locus_tag="VFC74_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HZK24153.1"
                     /translation="MTKGYFSLVLHAHLPYVRHPEYTYSFEEKWLFEALTETYLPLLE
                     SLTRLEQDKIDYRLTISISPTLAAMWQDEYLQGKYRHYLLSLLELADKEVKRTANDPV
                     FSPLAGHYLQRLQSAYDTFFTTYRADLAGAFRQLAAGGKLEIITCAATHGYLPLLLTN
                     PEAVYAQLFTAVEQHTQLFGQAPKGIWLPECAYDYKIDALLAELGINYFLVDTHGIVF
                     AKPRPAYGTYSPLLTPHGVAAFGRDVESSKQVWSKTEGYPGDFAYRDFYRDIGFDLPL
                     DYIGPYIHPDGIRTHTGFKYYRITGKTDAKEPYNLAQAQKKLAQHAGNFMFNRERQIE
                     YVAGEIDRPPIVVAPYDAELFGHWWYEGPAWLEDLFRKIDYDQEVFRPITPSEYLSLG
                     LPLQEAEPSSSSWGSNGYHEVWLNENNDWLYRHLHMAAERMSQAAVDFPHASGLQEEA
                     LNQLARELLLAQASDWPFIMTTGTMVQYAQESAHDHLVRFNRLWQQIYQNKIDEDWLR
                     KLQTKDNIFSQLDFRIYAPLQQTEKVMASLNS"
     gene            complement(21141..21644)
                     /locus_tag="VFC74_02065"
     CDS             complement(21141..21644)
                     /locus_tag="VFC74_02065"
                     /inference="COORDINATES: protein motif:HMM:NF027583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4912 domain-containing protein"
                     /protein_id="HZK24154.1"
                     /translation="MHAPDRHELNAGWTLPNSYGVDRLVLLPRDPHKLLAYWEVTPEL
                     EQKYEQQYPELWTRGSSMIKLTNVSTAKVQDFPINNLASNWYLAVNDADQRYYAQLGR
                     LLADGSFVNILTSNAVNTPRDSISSVLDPRWRMFAFWQHRYYRSMSGYSSYELICDKP
                     ITEGGPW"
     gene            21831..23054
                     /locus_tag="VFC74_02070"
     CDS             21831..23054
                     /locus_tag="VFC74_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24155.1"
                     /translation="MVRIALICRNLTIFDTDATLLQLAQEITQQGRECCIFTADKELK
                     VQKPVLFASFYVVSGGQLQQIAVTEQRQGALRIFTISAASSTLFAAAVAGMARHLLLS
                     EEKIDLLHLFSAADVCAALLCQQYFQIPFVYSVRELQEIKQRKQDFQDLSLTAPAQVF
                     TVGDFILNEQLAAQEAAYMIDETGNPRLWREFFCAYAGKYIEGKPFINVSYWSQLKST
                     PRQTRKSELLQKLLSEKDFVVACEKPPVQPPVQPPALMGEVLFVSSNLNKTERLSLLQ
                     AADYYLVENLQPENLLTAMAAGCIPIAPVQSFTQSLIHDEEQQSAQATGYLYQQEGWP
                     SVLQRACTDYRARPGQQQQLRQRGMMLIRDYFSIKTAAALYERIYHGSAPVRLPFIME
                     NNRQPTATGVNEVQL"
     gene            23051..25120
                     /locus_tag="VFC74_02075"
     CDS             23051..25120
                     /locus_tag="VFC74_02075"
                     /inference="COORDINATES: protein motif:HMM:NF020660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HZK24156.1"
                     /translation="MKKTAGRKINFILALHNHQPEGNFPDVFEKNYQLAYEPFIQELE
                     QFPELKVVQHYSGILLRWLQENHPNFMSRLRQLAANGQVELMGGAFYEPILVMIPDED
                     KVNQIKKQSRFLRENFQVTVSGVWLAERVWEPHLVRPLAEAGMHYTVLDDELFEKVGL
                     KEEELLRAYVVEEAGRRLAVFPINEKMRYLIPFAEPEEAIAYLAEMATPEGERVVVLA
                     DDGEKFGSWPGTGKRVYEQRWLQRFFKLLKENSDWLQVTTFTEYLQNYHPAGLVYLSA
                     GSYREMMEWSGGFWRNFLVRYPESNQIHKKMLHVREKLQSLPASPQKREAEEYLWAGQ
                     CNCAYWHGVFGGLYLNFLRSALYSRLLAAEEITDRALHGSEPWVELERKDFNYDGADE
                     VVVTGSDLAFIVAPEQGGALLELDFKPKRFNLFDVLTRSKENYHCSLYEQAQEICKTD
                     EVKTIHHISRVKEAGLEQLLTYDPYRRASWRDHFFPQQSDLLQAIAEVEIGDFANAPY
                     EIISATESNAAHLELERHGTVVLAGEVCPLLVYKALHYQPQEGELRYSYRLQNRGKKE
                     LELDFAVEFNINFLAGQASDRYYSAPGQILTNRQLNSRGRTANLTRFGLVDEWQGIST
                     MFRFDRPATVWRMPIETVSQSEEGMERVYQGSMILPTWKVKLLPHTPWSLTFTQSVSE
                     LVREVHK"
     gene            25117..26820
                     /locus_tag="VFC74_02080"
     CDS             25117..26820
                     /locus_tag="VFC74_02080"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HZK24157.1"
                     /translation="MKPLYLAFIWNQHQPFYQDTVKKEYIMPWVRLHATKDYYQMAAI
                     LRHYPTIRQTFNLTPSLVAQLEDYLKGADDYYLRVMKPAAALSVAEKRFLLQHYFDIH
                     WERVIAKWPRYQELLAKQGHCKEPAAVEEALASYTEQDYLDLQVWFNLVWIDPEVREE
                     DAELRALQTKGRYFTEADKRLVLQKQWELMRQVVPVHRQLAEKGQIELMTTPYYHPIM
                     PLLIDTRSALRASPGLRLPKTFSYPEDAAEQIMRAINQYRLIFAGWPQGVWPPEQAVS
                     PETLALFAASGFSWTVSDEDILARSLQIEFYRDCSGHVLNADQLYRPYLVKVGSREIA
                     MVFRDHHLSDRLGFVYHQMNIDQAVDDLVHRLHKIREAVSDCEGPHLVTIALDGENAW
                     EWYDNDKNEFLHKLYSRLSQDSLLQTVTISEFLRDYPPQRVLPQLYTGSWVDHNLTRW
                     IGSESKNQLWQLLLDTRRMLEEVRGTITAEQLMKAKENLLIAEGSDYPWWVDSMPYYL
                     SAPFEALFRKHLINVYRECGQEVPPALLRTVLCPKDGEAVWSEDDPLAGPTTMVPTIK
                     N"
     gene            26907..27944
                     /gene="holA"
                     /locus_tag="VFC74_02085"
     CDS             26907..27944
                     /gene="holA"
                     /locus_tag="VFC74_02085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HZK24158.1"
                     /translation="MQRRRYEIDALRLEREIKAGERRPVYLFYGEEDFLQSAAVSTVA
                     DYLLPGGLQDSSCTRAAGSDYSLEEVLDMVDTVALFSQGRLLIVQAAPYFIKGSRLED
                     AAVQRLLAYHQQKSSDSTIIFCTAEKVSAAKVVKELTSAGAVYHFEPLKWTPLMTWLR
                     ERVAVKGKKATTPVLQFLINRVGQDLRRLASELDKLVSYLGEQQTIDEQSILAVTSPG
                     AQANIFALIDAAVYGRTAFALSLLKNLLVLREPPLRILSMLTRQFRLLGEAKELLAAG
                     EDNIAKALSLHSFAAENLMKQVRCTDEARLNRAVELLLRCELDIKRGRIQPVLALETL
                     IVALGVDAPGR"
     gene            complement(28091..28378)
                     /gene="rpsT"
                     /locus_tag="VFC74_02090"
     CDS             complement(28091..28378)
                     /gene="rpsT"
                     /locus_tag="VFC74_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HZK24159.1"
                     /translation="MPNSKQAGKRMRVTAKRTARNRYVKSTVKTSVRGFRDTLAEGNL
                     DQAGDKLQAAVKTLDKAVSKGVVHKNAAARKKSRMTKHLNKAAAAQQAAAE"
     gene            28511..28852
                     /locus_tag="VFC74_02095"
     CDS             28511..28852
                     /locus_tag="VFC74_02095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HZK24160.1"
                     /translation="MEVNYVLGLIVRFIVSAIVLMLVSFLLPGFANMGFAVALVAAIV
                     IALLGFIVESVLGKSISPQNRGIVGFLTSAIVIYLAQYLVSGMEVTILGAALAALVIG
                     IIDAFVPTELR"
     gene            28863..29855
                     /gene="gpr"
                     /locus_tag="VFC74_02100"
     CDS             28863..29855
                     /gene="gpr"
                     /locus_tag="VFC74_02100"
                     /EC_number="3.4.24.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPR endopeptidase"
                     /protein_id="HZK24161.1"
                     /translation="MTLKQQNRSIRTDLAIEAHQMLVGEVEQEIPGVLVETEEKDQIR
                     VSRVHITSPEAEERMGKQQGKYITLEAPGLRQKNTELQEMVSRQFTKELLSLVELEDK
                     ATILIVGLGNWNVTPDALGPRVIKDLLVTRHILALQPETLGDGFRAVSAISPGVLGIT
                     GVETGEIIQGLVEKVKPDLVIAVDALAARSLSRLHTTIQIADTGITPGSGVGNKRLKI
                     SHETIGIPVIAVGVPTVVDAVTIADDLTSHLLEALMQEAGSDSPVYKVIQSIAEGDRR
                     AVMSEVLDPYGGRLMVTPKEIDTLVEDIARLLAGGINAAMHPAIQSEEVSKYLH"
     gene            30016..31080
                     /locus_tag="VFC74_02105"
     CDS             30016..31080
                     /locus_tag="VFC74_02105"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HZK24162.1"
                     /translation="MARRIHFFVRPNRRFRSSKMGRGKVWVAALAAVVLLMGGSYLYR
                     QQLEAVATFAATPQQESSPLLSFGKSLSLRIVSTVIPGFNDHDHALSEGEVSTAEDHY
                     LQKLALNDLRNPKNMIYAQIPYLGVVPETLINKPATDQPETPKITFPVRAELTGAGTV
                     LLYHTHATESFVPSSGKAFVSTDLSQTITQLGAELASLLQNEYQISVYHDQKIHDVPR
                     TGAYERALPTITELLKTYPNTALVVDLHRDGVAKKITTTTLNGQAAARILLVVGSRHD
                     DWQENKAKAEFMHHTLEKLAPGISRGIRERPLVYNQNVHAGCLLIELGGYQNSLEEVR
                     RTLPVLAEALALLYSSGQGQ"
     gene            31174..31410
                     /locus_tag="VFC74_02110"
     CDS             31174..31410
                     /locus_tag="VFC74_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24163.1"
                     /translation="MKKQTVLSVLLIFLFAFALFYAGLSLSEQALQQVSGTVREPAAL
                     SLSRDEAGTWVLTFAGRTLRLTRQLWQVFWRKNG"
     gene            31478..33274
                     /gene="lepA"
                     /locus_tag="VFC74_02115"
     CDS             31478..33274
                     /gene="lepA"
                     /locus_tag="VFC74_02115"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HZK24164.1"
                     /translation="MQQKNIRNFSIIAHIDHGKSTLADRLLEATGALSQREMSEQVLD
                     QLDLERERGITIKLQAVRLMYQAQDGQEYMLNLIDTPGHVDFTYEVSRSLAACEGALL
                     VIDAAQGIEAQTLANVYLALEHNLEIIPVINKIDLPSADVEKAKRELEEVIGLSGDEA
                     VLTSAKTGLGITEVLEAVVQRVPAPQGERAAPLRALIFDSHYDSYRGAIAYVRITEGT
                     LTERMKIKMMSNGKVFEVSEIGSFTPHMTRTGNLAAGEVGYVVAGVKNVKDTRVGDTI
                     TVADNPAVSPLPGYQKANPMVYCGFYPADSNDYVDLRDALEKLNLNDAALTFEPETSE
                     ALGFGFRCGFLGLLHMEIIQERLEREYKISLITTAPNVVYHATLNNGELLQIDNPTRM
                     PQAAELASVAEPFVSARVIMPNDYVGAVMELCQEKRGIFINMEYLDTTRVTLIYEMPL
                     SEIVFDFFDLLKSRTRGYASFDYELVGYRESKLVKVDILLNGAAVDALSFIAHLERAT
                     KRGRELASKLRELIPRQMFEVPIQAAIGQKVIARETIKAMRKNVLDKCYGGDITRKRK
                     LLEKQKAGKKRMKQVGNVEIPQEAFMAVLSID"
     gene            33274..34419
                     /gene="hemW"
                     /locus_tag="VFC74_02120"
     CDS             33274..34419
                     /gene="hemW"
                     /locus_tag="VFC74_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HZK24165.1"
                     /translation="MMGTAVYLHIPFCRAKCHYCDFLSYACSTSGSFTPQLYLQGLVR
                     ELAIRGAELISAGREVTTLYLGGGTPTVLEPPDMAELLSCCHRYLPLASPEWTVEANP
                     GTITPAAVRQLAKLGVNRISLGVQDLCDQRLASLGRLHNAQQARQAFLLCRDYFPSVS
                     LDLMAALPGQTSAQLLETLAAVCDWGPQHLSVYGLKVEEGTLLAAQVAAGQVQLPDED
                     EVLTMFLAGRQYLQSRGYEHYEIANYARPGHQCRHNLTYWSNQPYLGLGLGAHSYWQG
                     VRWENTRDPETYLTQLAAGQLPQAVQTTVTKDEAMEDTMMLGLRLLNGVSFADFQEKF
                     GQDLRQVFNQEIKYLQSLGAVECDEHYIRLSEKGLPVANVVFAEFLR"
     gene            34524..35552
                     /gene="hrcA"
                     /locus_tag="VFC74_02125"
     CDS             34524..35552
                     /gene="hrcA"
                     /locus_tag="VFC74_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HZK24166.1"
                     /translation="MTGLNERKQKILHAIVTDYIQTAEPVGSRALSRHYQIKLSAATI
                     RNEMADLEELGFVTQPHTSAGRIPTQQGYRFFVDTLMDQVALTNPEEKYLAEFYRQVQ
                     KMQEIDLIIQHTAKVLSVMTSYTSLILGPLFRRSAFKQMRIIPIDQERALVVLITDTG
                     YIKNKVITLPRALNQEELNQVIAYLNQQLAGKTIASLSAEHLRQLRHDLYARVDILNH
                     MMNMLEESAAEGESRVFLGGTSNILNQPEFKDMKKIKHLLTLFEQGERLTFMLETEME
                     GVLIRIGTENNLQEMDDCSLVTASYYLHNRPVGTIGVLGPTRMEYSRVITIIEEIASR
                     LNRLIEAL"
     gene            35566..36126
                     /gene="grpE"
                     /locus_tag="VFC74_02130"
     CDS             35566..36126
                     /gene="grpE"
                     /locus_tag="VFC74_02130"
                     /inference="COORDINATES: protein motif:HMM:NF010738.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HZK24167.1"
                     /translation="MNAEEKVKTTAEQAEAEETVQAEADAAAKTQTTDSTAAEEVTTA
                     IVQLQEKNEQLVSRLHRLQADFDNYRKRLANEKKEWHTQAICEVVRELLPVIDNLERA
                     NEAAGSLAALKDGVELIHRQLLTALSKQGFEKIEACGQEFDPNFHHAVMQVEGAEPEN
                     TVVEELQKGYLVNGRVVRPSMVKVAK"
     gene            36148..37977
                     /gene="dnaK"
                     /locus_tag="VFC74_02135"
     CDS             36148..37977
                     /gene="dnaK"
                     /locus_tag="VFC74_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZK24168.1"
                     /translation="MSKVIGIDLGTTNSCVAVMEGGQPEVIANAEGERITPSVVAFKK
                     DGERLVGQVAKRQAITNPDRTIMSVKRHMGTDYKVKIDDKNYTPQEISAMILQKLKQD
                     AEAYLGEKVEQAVITVPAYFTDAQRQATKDAGKIAGLEVLRIINEPTAAALAYGMDKG
                     EEQTILVFDLGGGTFDVSILELGDGVFEVKATSGNNRLGGDDFDEKVIQYLAAQFKKD
                     EGVDLTADRMAVQRLKEAAEKAKIELSSVTTTNINLPFITATSDGPKHLDITLTRAKF
                     NELTADLVEATMTPARNALADAGFKPADIDRAILVGGSTRIPAVQEAIKNLIGKEPHK
                     GVNPDEVVSLGAAIQAGVLAGEVKDVLLLDVTPLSLGIETLGGVFTRIIERNTTIPTD
                     KKQTFSTAADNQTQVEIHVLQGERSMAAGNKTLGRFVLDGIPPAPRGIPQIEVTFDID
                     RNGIVSVSAKDLGTKKEQKITITASTGLSDNDIDAMVKDAEKFAAEDEQRKELVEARN
                     EADNLVYSTEKMLKDLGDKVDAAKKTEVETAINQLREESAKEDVAAIRTAIENLTKYL
                     HELSTQMYQQEQAQAEPNAAGAETADDTVDAEYEVVDDEDDKK"
     gene            37997..39130
                     /gene="dnaJ"
                     /locus_tag="VFC74_02140"
     CDS             37997..39130
                     /gene="dnaJ"
                     /locus_tag="VFC74_02140"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HZK24169.1"
                     /translation="MAKRDYYEVLGVSTEASAEEIKKAYRSLARKYHPDVNRDDQDAE
                     KKFKEAKEAFDVLSDPQRRQQYDQYGHAAEEGGFGTGGFGQGDFSGFGGFEDIFDQFF
                     GGAARTRRPTGPQRGNDLRYDLEITLEEAALGKEAKINIPRAETCDTCGGSGAKPGTQ
                     PETCSHCHGTGQQQVTRNTAFGRFVSVQACVACGGTGTIIKERCLDCQGNGRVQRERK
                     IDIKIPAGVETGSRLRVANEGEAGTRGGPPGDLYVVLHVRPHKLFDRQGDDIIIEVPI
                     SFAQATLGTELEVPTITGKARVKIPEGTQTGTSFRLRGKGIPHLRGYGQGDQHVKVVV
                     KIPKKLSSKQKELLREYAKLAGEDVAAGDKGFLNKVKDALGGK"
     gene            39143..40087
                     /gene="prmA"
                     /locus_tag="VFC74_02145"
     CDS             39143..40087
                     /gene="prmA"
                     /locus_tag="VFC74_02145"
                     /inference="COORDINATES: protein motif:HMM:NF018076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HZK24170.1"
                     /translation="MRWLELIIHTRKESSEAIAAKLLALGTGGVSFEESWLWEQAKKD
                     GLDDLFPTPSAGGLQDDIAILRGYFPVSFLGSASEKELHSFLLALPSFGLPEADLKWR
                     YVDDVEWENRWKDYWFPTPVGKKLLIVPTWRQVEEDEDRLKILLDPGAAFGTGSHETT
                     QLCLGLLEMVIAGGDKVLDLGCGSGILSIAAHALGAEAVLGLDYDEVAIRISRENAIL
                     NGSPEIKFVQRDLFKETSWQTLPTADVIVANLTADILLKIKHHLPAVLRPGGKIILSG
                     IIKERAEEVMTTIFTQGYQLLAERSAGEWVALLGEREA"
     gene            40084..40821
                     /locus_tag="VFC74_02150"
     CDS             40084..40821
                     /locus_tag="VFC74_02150"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HZK24171.1"
                     /translation="MNRFFYQTTQREAEIVRLQGDDAHHLRRVLRAAVGEQIELCDEQ
                     GLCHLARVTQITKDEVECALASALPSSEPQTKIELAFGLLKGEKTELVLQKATELGVS
                     SLRPFFSERTVVRADKKQGARYERWQKIVRAAAAQARRAQIPAVVLPCQWSSLLTKFV
                     DYDQVILFWEAELEQPLAQALRHCARGARILLLTGPEGGFSEVEVRGATAAGAEIVTL
                     GPRILRAETAAITAVALTMYQAGEMRR"
     gene            40818..42122
                     /gene="mtaB"
                     /locus_tag="VFC74_02155"
     CDS             40818..42122
                     /gene="mtaB"
                     /locus_tag="VFC74_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HZK24172.1"
                     /translation="MKMPTAAYYTLGCKVNQTDTAALQQLMEQAGYTTVQFEAKADVY
                     VINTCTVTHLGDRKSRQMIRRARRRNSEAVIVVAGCYAQVAAEDVMSMPAVDLVIGTH
                     SRERLPELVEQAKLGRINSVTPLEGKVAFDNLAVGQSPEHTRAFLKVQEGCRQFCSYC
                     IVPYARGPLYSRPLAEAVSAAQKMVAQGFREVVLTGVHLGCYGFDFTEDLALSDLVRA
                     LAAVDGLARIRISSIEPTEVTADLIEVMLDNPNVCHHLHIPLQSGDDDVLQRMNRKYN
                     KMEFLRLAEWLRMQISDIALTTDVMVGFPGETEEQFSNTMQVVRKAAFSRLHVFKYSP
                     RRGTPAAKYPQQVPPPVKNERSQRLTELGQELASKYQQGFLEQEVNVLFEEEVGANQV
                     AGLTEHYVRVKAAGSDQMLGKIVPVRITALTAEGLLGILSES"
     gene            complement(42225..42350)
                     /locus_tag="VFC74_02160"
     CDS             complement(42225..42350)
                     /locus_tag="VFC74_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015616689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HZK24173.1"
                     /translation="MKKKLLSLLASLLTLVAVSGAASACWAILYQPKLPEALKKK"
     gene            complement(42451..43065)
                     /locus_tag="VFC74_02165"
     CDS             complement(42451..43065)
                     /locus_tag="VFC74_02165"
                     /inference="COORDINATES: protein motif:HMM:NF016526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HZK24174.1"
                     /translation="MKWLVDYLTRELKLAHDQREIIEYGLSSLVYTALSIFSALLLAK
                     LFGIFKETAAVITVIMLYRKVSGGAHISSAHGCLIIGTIASLILAYLTSLWAPFYAAT
                     PAAIILPVCIACYLAYRYVPAAVPQKPITSIAQIKILRLLSFSFILLWAVLGFVTVQL
                     PQVWSYYYFAGNLGLLWQSFLLTPLGYHLLAALSPLFYKKHEID"
     gene            complement(43086..43565)
                     /locus_tag="VFC74_02170"
     CDS             complement(43086..43565)
                     /locus_tag="VFC74_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24175.1"
                     /translation="MEAMPLITLILLAFPEGVLTSSVGLTLINFRPHWPGAVRVGCIH
                     MIISFMMRRLLAPGIHTIVITVALIGIIMYSYKLNFKKSFSTTILGFVFLVLGESITM
                     PLFTRAFSVTIAEILANPWLRVAASVPTQLFLFLVFVLAYRLRGFHKAAAYRLSRRY"
     gene            complement(43677..44777)
                     /locus_tag="VFC74_02175"
     CDS             complement(43677..44777)
                     /locus_tag="VFC74_02175"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK24176.1"
                     /translation="MDNNASGLINRFFSERSFPMRTKVGVYALALTATLALAYLTLSG
                     SFSDSETRLILYLFHISIFTTTFFCGLIPGIVTATISTIVAINSLGENWLMLNKITFK
                     EAEIFPFIALYFLVAITVDLFSEHIEKLEKQLVEIEQLHETTRQMEKLALAGQIAAGI
                     AHEIRNPITVVHGYLQLLTSKQPVVDENYQLMLDELKRANQIITDFLRFSRPGELRKV
                     PVQLNHILETTVSLMVGQEIQNDVKIKTKLADYLPIILLDRSQMLQVFLNLFTNAIQA
                     MPAGGIITVSSEFDQEANVIRVIIKDTGTGIKPEAMPQIFDPFFSTKDQGTGLGLAIT
                     QTIVAAHDGQIEAQSKLKEGTQFTLTFPVPVE"
     gene            44947..45489
                     /locus_tag="VFC74_02180"
     CDS             44947..45489
                     /locus_tag="VFC74_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24177.1"
                     /translation="MARNKMFVVEARRMLPLLFLLVLLVSLSVYDSFRASPTAAPEGV
                     AKENEVSFITADKGEREDNPTFRLAVDEEGWTEVAEEWAVTLPEYPFQPQHEVALFAL
                     HAEVQNVQTSKVGENELVVEVRVNPKRDYYQVVTLPAADVMLEAGKVTWKFVNKKGSE
                     IEQLVVEATQQTIAEDTPQK"
     gene            complement(45525..46643)
                     /locus_tag="VFC74_02185"
     CDS             complement(45525..46643)
                     /locus_tag="VFC74_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24178.1"
                     /translation="MATGKSTNFFAGTNSYQGFKSLFHYIADERTKRLVILKGGPGTG
                     KSTIMKQVMQKALDAGYDTENFFCSSDHASLDAVSIPDLGYVIVDGTAPHIVEPIIPG
                     AYDEILNLGDFWDASALQPYKKEINDFMQENSNYYKQAYQYLKEAAVVMEKLRWLMAQ
                     AMDYQAVDAMTHQLLTELAAALPASTRAAKERHLFGSSITPAGLVNFYPSILQNTERF
                     YLLSGDPGCGKSYLLEQIRQTVNRAGHDTEVYRCGFDPDHIDAVVIPVLKTAFVKITY
                     PHTFSVNPVQVVKEQATIALSRYANTTILKKYSGERSESSERLWFLLGKAVEMIRGSK
                     RNHDQLENYYIKAMDYSRLDAVRQQLSARILKQEAKDK"
BASE COUNT        12569 a         9907 c        11689 g        12568 t
ORIGIN      
        1 gatttccacc tccaccttca gcaactacgg aaccgactag agttaagagc atcgttaaga
       61 ttaggaagat tgctaggagt tttcttttta cagttttcat tttattaaac ctcctttttt
      121 taccccggcc cctgttcccc ccaagcaaca tgccagagcc agattaaaca aatctcctcc
      181 tttcacctgt caaatattaa gacttaatga gttatcgtac agaaattgtt gtaatctgtg
      241 cggatgagta ttgtaaatcg tcaatttttg gcaataaaaa agcacacggg tataaaaccc
      301 gtgtgcagcc ggtatcactc tatctcccac gcctcataag tgacctgatc acgaagcggt
      361 gttcattgaa tccgcacact acagcaacag ttattgctag agcaccttca ttataagatg
      421 ccaaagtttt actgtcaaga ataagttgaa aaattcacat aattaaatat ggtatagcat
      481 atcattgtta tttgtcattt ttattagact tcatttatgc cgtaaataga gtggttgcta
      541 agtgcactgg tgctcttgca ttccctcctt taatccgtat ataataggaa gaaagcctgt
      601 ggacaggcaa tctttggtac tgacggtaaa ttatcttgct tgcagatagc aggtgctaat
      661 gaagccagtc taaaagcttg tcagcggaaa acgaaagttt tttggagatt tgaagtatag
      721 gaggcagatt gtggtggctg agtataatcc aagtgttatc gaacccaagt ggcagaggtt
      781 ttgggaagaa aatgacttaa atcagacaga agaagataga cagaagaaat attatgttct
      841 agagatgttt ccttatccct cggggcgttt acatatgggg catatgcggg tttactctat
      901 tggtgatgtt ttagctcgtt ttttacgtat gcgcggcttt agtgtacttc accctatggg
      961 gtgggatgcc tttggtttgc cggcagaaaa tgcagccatc aaaaaccaag ttcaccctgc
     1021 tgcatggacc tatgccaata ttgaggcgat gaaaaaacag caaaaagcgc tgggcattag
     1081 ttatgattgg cagcgagaag ttgccacttg tgaccctgac tattataaat ggacacaatg
     1141 gttattttta cttttatata aacgtgggtt ggcctataaa aagcaggcgg gtgtaaactg
     1201 gtgtccaaac tgcagcacag ttttagctaa tgagcaggtg gaagatggtg gctgctggcg
     1261 ctgtggtaca gaagtgaccc acaaagattt ggaacagtgg tttttccgaa tcacagacta
     1321 tacagagcgg ctgctagaag atttaaagct attgtctggt tggccggagc gtgtgcgcat
     1381 gatgcaggag aattggattg gcaaaagcga aggcgctgag gttcgtttta aagtcaaagg
     1441 tagagaggat gaaatctctg tttttaccac tcgtcctgat actttgtatg gcgtaaccta
     1501 tctggtgctg gctgcggaac actctcttgt tgaggcatta actgctggta gtgaacggca
     1561 agcagaagtg caggcttttg ttgaaaatac aaaaaaactt aatgatatcg ctcgtgcttc
     1621 gacagagatg gttaaagaag gtgtttttac cggtgcctac tgcctcaatc cggtgaatgg
     1681 tgaagaagtg ccaattttag taggtaacta tgttttaatg ggttatggca caggtgcggt
     1741 catgggcgtg cctgcccatg accaacggga ctttgagttt gctaaaaaat atcagctgcc
     1801 gctgcgtgta gtaattaccc ctgaggatca ggaattgagt gctgctgatt taaaggaagc
     1861 ttatgtagat gcgggtgtct taattaattc cgcccagttt aatggtaaaa ccagtgaagc
     1921 agcccaaaag aaaattgccg cttacttggc cgctaacaac tggggagagg ccaaagtaac
     1981 ttatcgtctt cgtgactggc tgatttcacg gcagcgctat tggggtgcac ctattcctgt
     2041 tctttactgt gagcactgtg gcactgtacc tgttcctgaa gagcagctgc cagtattgtt
     2101 accggaagat gtggtcttta aagcgggcgg tcaatcacct ttagctgaac atgaaggatt
     2161 tttgcacgcc acttgtcccc agtgtggtgg tcaagctagg cgagaaacag acaccatgga
     2221 cacttttatt gattcttcgt ggtattattt tcgctacacc gatgctcaga atgagaacct
     2281 tccctttgcc aaggggcagg gagacgcctg ggtaccggta gatcagtata ttggcggcat
     2341 tgaacatgct attttgcatc tattgtattc gcgtttcatt actaaagtct tccatgatgc
     2401 cggctacact gcagctgttg aaccttttac ccgtttgtta gcgcaaggca tggtgaataa
     2461 agatggtgca aaaatgtcga aatctaaagg caatgtggtc agccctgatg aaattattga
     2521 aaaatatggt gctgatacag ggcggttatt cattctcttt gctgccccac cggaaaaaga
     2581 tgtggattgg agtgaccgtg gcgtagaggg cagctaccgt tttctaaaac gcgtctggcg
     2641 tttagttgat cgctatgctg acgtcctaca gcagccagtt gaacagcagg tcgctgcgga
     2701 tgatgctgat caagcactac gccgtgcgat gcataccgca ctgaagaaag tgactgagga
     2761 tattgaggag cgtttcaact ttaacacagc aatttcctca attatggaag ctgtcaactc
     2821 tgcttatagc tatatgcatg aaaaggaaga tcatattcac catgggatga tgagggaagt
     2881 attggaaatc ttagttgtaa tgctggcccc atttgccccc catttaggtg aagaattgtg
     2941 ggagcgcttg ggtcaccaaa ctagcgttca tttacagtcc tggcccacag tggactctac
     3001 agctctccaa gtggctgagg tggaaattgt ggtgcaggtt aatggtaagg tgcgtgacca
     3061 tttagtcgtg cccacggggt taacccggga ggcgctgcag gaactagctt tggccagtgc
     3121 caaggttgcc cagctggcag cagacaagca agttgtgaag gtcatagcgg taccagataa
     3181 actggtgaat atcgtcgttc gctgacatgg cccaggtaat aatgtcaaaa aaaagatcaa
     3241 cctaggcagg aaaattgtgg ttatcaagag aaaaacactt taacaaagct tgctctacat
     3301 aattagggca agctttttta tcagctagga aactattgca accatgcatt gccttcatgc
     3361 tagctgctgg agtttttagg aaggtccgac ttattgctgc gaggtggcca agttcgcaga
     3421 cggcggcagc acctaaagaa aaagaaagac gcggcaggga aaaataggca gcgcctttct
     3481 tggaaatgtc aggaggcttt tgtgatgttt caaatgacca gacgagagca gcttggtgct
     3541 atcatcttgg tcgtactttt attgctaggt ttattgctac gctatttatt attgccaaaa
     3601 cctgtagcgt tggaggttct cccgccccct gaaacaccgc tggatgaaga acagccgcaa
     3661 gcagaaattg tggtgcatat cgctggcgca gtacgtactc ccggggttta taccctgccg
     3721 gcaggggcgc gggtttacat ggcgctggag gcagcaggag gggtgctgcc agaagcggat
     3781 gagcacgccc ttaatcttgc cgagccactt tttgacggac gacgtattac tgtgccgttc
     3841 cagggtgagc agggtgccgc tgccgctgcc tctgataatg ataaagtaaa tattaatact
     3901 gctcaagcgg ccgaatttga gcagctgcca ggcattggtc ccgctaaagc cgctgctatt
     3961 attgcttatc gcgaggaaaa cggtcctttt caatctgtag acgatttaac ccgtgtttca
     4021 gggatcggta ttaatacttt gaatgtaatc aaggactacc tcacacttta ttaagtcttc
     4081 agcttcagga cggcgagcag cttctgtaaa agctcccttg gtgcaggtat tttgatgccg
     4141 tagaattgca taatggccaa cgtagacgca agcagccaca agacaagaaa aacaagcaat
     4201 tcccgccaca ttttattcag gattaaacgt ggtgtttcaa ataaggctat ggctagtaaa
     4261 cctaaaacca gtaagataaa cagtgcttgc cacctccaac tactcaccgc ggggcagagc
     4321 aggattggcc tgcatacctg cgcggcggac atctgaagta accactatct ccacttttag
     4381 cttgctaaat tcttctggcc agttgtcctg caggcggcta aagagcttcg gttcatgacg
     4441 gtttaaaata ctgccgaaac caacaagatc tgtctgcagt tgttgcatcc gatgcagtgc
     4501 tttttccatt tcctgtttaa tgacggcggc attggcgctg ttaacttttt tcatcatttc
     4561 cggtgtattg atcttcgttg tgctggtttg ggagcggata tccccttcgg taaccacttc
     4621 aaccgagaag gaaagctggc cattttttat ctggggctta atctttgtct ccgcacggga
     4681 aacgtgcaaa gtgatgatct cctttggttt ttgaggatcg gaaatggcaa gataggtacg
     4741 gctagtttta cctaaaaacc agagaaagcc gcgggtttcc tgggcgttca ttagcccagc
     4801 caatttttga ttacgaaagg cagctagccc cgccaaagtc aatacttctt ttgctttttc
     4861 tccgccgccg ccttcactgc tgtcgctgcc gccactgctc tcttcctgtc cgccaggttt
     4921 ttgttcaccc tgtaagccgg gaattatttc ttctagaggt gagccctttt gtaaaagcgc
     4981 agtataggcg gcctgccctg gtgttactac tttctgcaga aacttatatg cgggcagcgg
     5041 ttcagtttcc gagatgaacc gcgcctgctc aatcatgcca attatttctt cggtgatgcc
     5101 tatttctaaa cgttcagtag tagtcagcat ctgttgggca aagccattag tatatagcaa
     5161 ataagcatta aggcgtactt gtgggttgcg gataataaaa tcaaaaacat gtgccagccc
     5221 atgtttggcc atttcttcac caatcagaat tacgctggcg tgagagagaa acaactgtcg
     5281 ggacaagttt aaactggcct ggtgaagcgc caggtccatg cttgcaccga cacctgaaaa
     5341 aagctgcgtg ggttcgccgc caccgccgcc gccgccttca cctcctgcca catctgaggg
     5401 gagggagatg gcaaaagtga ctagataagg agacctgcgc cccggctggt agtcaatggc
     5461 aatggcgcgc acaagtgcta attcttctac ttcaatctga tcccagcagc ctgccgtaaa
     5521 aaggctgagg caaaagagga gcagacataa aatggtaagt tgttttttca ttgcatcctc
     5581 cccagctgtc gcttgtgtaa cttttctttg agccaggtta gtactaaaag aaaaagggga
     5641 atgccaaaat acatgagcag cacccttggt gaggtggtgc tcacagacgc ttctagttca
     5701 gtgatatttt caaataggga aaaagaccag acgacggcaa tagtgcctag ggctaaaacc
     5761 acggggcgta tttcttttaa gccggttatt tgtgccaaac ctaaacaggc acaatacaag
     5821 tagaaagtag ttttagcaaa aaccccggcc acccaaacca ccataattag aacttcaaag
     5881 cgtgataaga tttgtgccac actgatggaa cgcacaaaca tcaggatggg aaagcgagta
     5941 acgagtaaaa aataggggcc aaaggtggcc aaaagagaga tcaggccaat ggctaaaagg
     6001 ccgttggcaa aaactgctgc ggcggctcca gcagccaaag cccgctgcgg ccgcgtcagg
     6061 aaggggataa tgacagcaaa agtgatggta tccactgccc atacttccgg gataagtgat
     6121 gtcttgagca gagaaaggaa ttcaacccta aacaggggct gtaaatatcc cagctcccag
     6181 ttgcccagtg aaagcagcaa aataaaatac aacgagccaa taactaagag gatgataaat
     6241 tcattcatgc gggccagcac ttccagccca gcaaacacag tccaggcggc aagaaagatc
     6301 aggctgaggg aaaaaactga cagaggcgta ttgggcataa aggaagtagt cataaagtca
     6361 ccgaattccc taatcacaat ggccactacc cgcagaaaac tccagacata cagcagtcca
     6421 actactttac ccagccattt gcccaagagt gtctcggtgt actgaaaaat agtctcttcc
     6481 ggatgtttat tgcccagcca acaaactata gaaagagtaa ctaaccccca tattgtgccc
     6541 agcaaaacag ctaaccaggc attttgtccc tgttgttcga cggcaactgc cgggaccagc
     6601 agaatagagg tggctaataa agagatgacc aatagttcac cgccttggcg aggggagatt
     6661 ttaccctttt ccaggctgcc gatctttgct ttcttcatgc gttagttcac gctcctctaa
     6721 tggggtgtga cgcgcccaag tgttagttgc ttgtcgccga cgttgttgtg gatctagtaa
     6781 gcggggacgg aagatgttcg cccaccaagg tggacgcacg atagttttga ccatatctct
     6841 gaatgttcca tgcactaaag gtgtcagata ggggacacca aaggattgta aacctgctaa
     6901 atggatgaga atgataatca aaccgaaaat aacaccatat agccccatca tggcccctag
     6961 aataattaaa ggaaagcgca gcaggcgtag ggcaacagaa gcattaaagg cagggatggc
     7021 gaaggtagaa atagctgtgg cggccacaat tatcaccatg ccaggagaaa ctaaaccggc
     7081 acgaatggcc gcatcaccaa ttactaaacc accgacaata ctaacagcct gaccaactgt
     7141 tttgggcaga cgaatgccag cttcccgcag cacctcaaag gtgccttcca tcagcaaggc
     7201 ttcagctacg gcgggaaaag gaatgccttc ccgagcagct gtgatattga gtagtaggga
     7261 agtgggcagc atttcctggt ggaagctgat tacagctaca taggtggcag gtaggattaa
     7321 agcaatattt aagacaaaaa ggcgcagcag gcgaaggaat aatgccacat aaggtctttc
     7381 ataaaaatct tcagaagcct gaatgaattg gaagaaacta gtgggaacaa tcagcatgaa
     7441 gggagtatta tctgtcatca cgaggacgcg tccctctaga atggctgccg ccgccttatc
     7501 tacacgttca gtgtgctcca gctgcggaaa gatagagaaa ggcgcatctt caatatattc
     7561 ttcgatgtag ccggattcta aaatggcatc ggtgtcaata gcagctatcc gctgccgcac
     7621 ttctgctaat gtctcctggt taacaatatc attgatgtag accagattta tatcggtagc
     7681 tgtccgccgg cccgctttca tcgtctcaaa acgcaaattt tcatgtttaa tccgtcggcg
     7741 cagcagcgaa gtattaatgc gcatggtttc tgtgaagcct tcacgcgggc cgcgtacggc
     7801 agcttcagtg acaggctcgg cgacggtgcg tttttcccac atccgcgtgc caaacacaat
     7861 gatccgctgt tcgccgtcaa tgaaaagagc tgtatcacca gaaagtacca tttttagggc
     7921 ttgtgtcagg ttagatgcct gggagagttc actaacagca gtgatatgtt tattgaggag
     7981 ttcaaaggcg ttactggcgg tgagcttttc cgaatattca ttggccagcg tattggaaag
     8041 gatcggcagc ataatattat cgttaatcat ttttttatcg acgagcccgt cgatcaggag
     8101 taaagcaagt ttaacctgat ccttgctggt attaaactca cgaattacca cgtcattgct
     8161 ctcgcccagc ctttttttaa tcagcgccag ccgttcactt aagacaggcg tatcgtcatt
     8221 ttctggtttg cgggcagctt gtttattatt ggtcattggt ggttttggtt tctctttacg
     8281 ggcaactaat ttttttaaaa aagtaaatgc gttagccatg atacctccgt aactaagcag
     8341 aatacttact aaacatccct gttcaagaaa cgaaattaat gcctagcaag ccagtgagag
     8401 tgaagagcaa agggaaggaa aatgcgctcc atggcacgaa tatacctgtt aggaggtacc
     8461 accactatga agcgaatatt ccccttattg atcatatttg ttgtgctgat gctagcatgg
     8521 ttggccgcag gcagctatct tgattggttg tggttttcac atttaggctt tgccagtgtc
     8581 tatcagacag tactcacctc ttcctgggca gtcggcttgg gcagcttcct gtttttcgca
     8641 atttttctct tctgcaattt actttacgtg caaaagtcca tcctcgggat gccaaattta
     8701 gtcttacgtc acttgttaat gaatacacgt ttcggggatt tattgacgcc ccagcgtctg
     8761 cgttcagtct tcttgggttt agcgctgcca ctcgcctggc tgacaacagt tccttttcgt
     8821 aacgcctggc tgacagtacg tttatttacc gctggtgcca atactggggt tactgagccg
     8881 atttttaatg ctgatgtcgg cttttatttg tttagattac ccctttggga gctgatttat
     8941 caatatttac tacttatctt tatcgtcact cttttagttt gtggtgtgct atatctgttc
     9001 atacgcccgc cgcagcagtt aggtctgcgc actttgtttg ccacacgtgg tcagtttcat
     9061 ctttcccgac ttttagccgg cacgctttta gtacgggctg ggggctaccg cctgcagatg
     9121 tatcgcttgg tctatgcccc agatggtgtg gttttcggtg ccggttatac cgatgtacat
     9181 gccctgctgc cagcatactg ggttatgctg ctgttagctg tggtgggagc agtgatccta
     9241 atactaaata cgaaactaaa gcagagccgt ctgcttacag gcagtattgt cagtataatt
     9301 ttagcctctc tggtgctgaa taatcttgta ccactggcag tacaaaagtt tttagtagaa
     9361 ccgaatgaat ttgtcagaga agagccttat ttgcagtata atattgattt tacgcaacag
     9421 gcttatgggc taaaaaaaat tgaagagcgg gagtttccgt taaacgaagc attgcagtat
     9481 gaagagttgt tggcaaacga aacaactctt gacaatatcc gcttgtggga ttggcgtccc
     9541 ttggggcaga catttaatca attacaaagc ttgcggcaat attatacttt ccatgatgtt
     9601 gacacagatc gttatctgat taatggtgag caacgccagg taatgctggc agcacgggaa
     9661 atggttactt cacaattggc agcacctacc tggattaata tgcatttagc ctatactcat
     9721 ggttatggtg ctgtggccag tcctgttaat gaagcgacag gacaacgcct tcccaaactg
     9781 ttcctccacg atttacctat taagggtgaa agcgacttgc aggtagatgt accacaaatc
     9841 tattatgggg aattaacgga taattatatc tttaccaaca ctaagacgaa agaatttgat
     9901 taccccatgg gtgaaagtaa tgctgtgact agttatgatg gtgagggagg tattcccctc
     9961 gggggtccct tgcgtcgcct tctttttggt ttgcgttttg cggactacag gatacttgtt
    10021 tctaatgaat taacggctgc cagtcgtctg cactttaagc ggaatctagc tgaacggctg
    10081 cacgaactga tgcctttttt gcagtatgat gaggatccct atctagtggt ggctgatggt
    10141 cgccttttct ggatgattga tgcctatact gtgagcaatc gctatcccta cgcacagcca
    10201 tatagtaata ttaactatat tcgaaacccg gtaaaagtaa cggttgatgc ctatcatggc
    10261 acagtacatt tctatgtttt tgatgctgac gaaccactgc tggctgctta cagcaaaatc
    10321 tttcccacag cttttacgcc agcggcagaa atgccgcctg ccttggcaga acatgtacgc
    10381 tatcctgaaa ccttgtttaa gctgcagtca gaagtctatg ctacttacca tatggataac
    10441 agcaatgttt tttataataa ggaagatcgt tggcaggttc ctcaagagaa gtatggtaat
    10501 gaacctattg aagtggaacc gtattatact atcctccggc tgcccgctga agctgaagct
    10561 gaatttattt tgatgcggcc atatactatg tatgagcggg ataatatggt agcgtggtta
    10621 gctgcccgct cggatggtga aggttatggt cagctggtag tttatcagtt tcctaagggc
    10681 gagcttgtct atggaccagc gcaaattgaa gcacgcattg atcaggatac taccatttcg
    10741 cagcagttgg cgttatggaa ccaacaggga tcccgagtgg ggcgaggtaa cttgctaatc
    10801 ttgccggtaa acggtaccat tctctacgtt gaacctattt atttgcaagc ggagcaaagc
    10861 gaactgccag aactagcgcg cgtgattgtg gcgtatggtg atcgggtagt gatggaaaat
    10921 accctagagg aagctttagc cgctgttttt ggcttacaat taggtgatag cgagcctgat
    10981 ttgcctgaaa agccgcctga gccgcctgaa acagggggac tacccctgcc cgcagctagt
    11041 ttggagacac tgatcaggca ggcagcagaa ctgtaccaag aggcggagat ggcccttaaa
    11101 gcaggtagtt gggcagagta tggtcgtcta caagaggaat tgggtcgtgt gttagaaagc
    11161 ttacaagaat ttagtctgca agattaaaag ctgattagct gtacatgcaa gcgcttagca
    11221 aaaataaagt tggagggaaa gatgaaacaa attgtgattt ataccgatgg cgcctgttcg
    11281 ggcaaccccg gccctggtgg ctatggcgcc gtgctgttgt acggggaaca tcaaaaagaa
    11341 gtttccggcg gcgaagcaaa gacaaccaac caacgcatgg agctgcaggc ggctgccgca
    11401 gctttagaac aactcaaaga accttgtcaa gttaagctgc atagcgacag cgcctatttg
    11461 attaatgctt ttaaccagca ctggtttgta aaatggcagc aaaatggttg gttaaatagc
    11521 aaaaaagagc cggtccagaa tcgtgatttg tgggagagac tgctggcatt ggctgagcaa
    11581 cacaaaatag aatgggtgaa agtaaaagga cacgccgacg atgaattaaa taaccgttgt
    11641 gatcaattgg cgcgtgaagc tattcctcgt gcctaaagga cagttgccag ctcaagattt
    11701 gccccctgct tataccgcct ttttaagggt tggcgccggc aaattgtcct acacctgaag
    11761 atttcaccag cggaactttt attgtgtaaa cccgtctatg tagataaagc agagggggta
    11821 tgaaaatgac aaagaagcaa tttttagggt ttttagtggt gatactttta attttagccg
    11881 gttgtagtgc tggcagaggc acatcagata acgcggtggg tgagggcggc atggcgggcg
    11941 ctccagaaga aaattttggg aaagaagcac aagagcgagc tgacgctgac ggcagtttgg
    12001 gcgaagcaga gcaaaaactg atccgcacag ccagtttact gctcgatgta gaagtactag
    12061 atgatgctat tgagactgtc tctgttatag ttaaaggagt cgacggtttt atcgccaaat
    12121 caaatgtcta tggtgctgcg gaaaaccgca gtgctgaact gaccttgcgc ttaccggctc
    12181 aacagttaga tgctgtgctg gacgagataa aaacaatggg cacagtacga cgcagcagta
    12241 ccggcagtaa cgatgtaacc ttgcagtacg tagatttaga agcgcgcatc cgcaatttgt
    12301 cgcggcagga agagcgctta ttagacgttt tagcgcaggc ctctacagta gaagatattt
    12361 tacgtgttga acaggagttg gcacggattc gcggtgagct ggaggcacac actgccgaat
    12421 ttcgttatct gaaggatcgt gttgattatg ctacggcaga tgttttcctt tatgaaacaa
    12481 gtaccgctag cagcacgatt actactactg gcctcacagg tgtttggcag cgtggcagca
    12541 ccggttttat taaatctatc aatgccatgc tcacaggcct cggcaacttg ttagtctttc
    12601 tgtttacttc tttaccatat ctgtctattt ttgctctaat cggtgtaccc atttttcttc
    12661 tagtacgtaa gttcatggcc aatcgcagcc gctcctaaac ttctagagct aaaggaggag
    12721 ttgatggtga cctttttgcc atcgctggtg ctgacctttg ccgcaggcat cgctttggcc
    12781 aagcaatgtc ccttatcttt gtggagctgg atatgtttag gcacagcagc gcttgctgca
    12841 gctattgttt taacattaaa aaagaagcac gctgctgcgg tgcttctttt actttttctc
    12901 ggcttaggga tactcgttta tcagcatgct catcgtgccc tagggcagcc tttagcaccc
    12961 ttgtggggtg cagaggctga atatcagggc tacatacaag aagtactaag ctgcagcgag
    13021 gagcaggccc aatttaactt ttatctggag gaggctgtcc tgccagagca aaaacagctg
    13081 caggtgggag cattaatccg agttagcgtt tatcgtccgg cagcctcttt ccagccgttt
    13141 tatggtctgc ccttaaaact gcggggtaca attaaagcag cgtcaggaca acgcaatcct
    13201 ggcggcttcg actacgctaa ttttctggaa acagaaaatg ttagtggcct tatgtctgca
    13261 gctccttggg agcttactgt tttggctggt gagaagggta acccggtact agttttcttt
    13321 gctcggctga gggctaaagc aataattcta ctgcagcatt atttaccaga ggaggaagca
    13381 caactggcgg ctgcgctcat tttagggcaa aaaaaggagc ttagtgagac gcttttaacc
    13441 gcctatcaac gtttgggttt agcccatctg ttagcagttt ccgggctgca tgtgggcttt
    13501 gttacggctt ttgccttgtt tgtgtttaca cgtcttttca gctcacggtg gccattgcta
    13561 tcgtccttgt tggcagcttt atttatttta gcctatgttt ttctcactgg cggacaacca
    13621 ccagtctggc gggcggcact aactgtgttg ataacctatc tggcaaggta tagtggacgg
    13681 gagagtgaag gtctgcagac gctgtcactg gtggcgctgc tgctgctttt tttccgtcct
    13741 ttgtggcttt ttagtctagc tttccagttg tcctttgctg ctaccggttc cattctgctt
    13801 ttagccccgc gcctgcaggg tgcttttagg tggtgcccaa aatttatggc gtcatcttta
    13861 gctgtcagta tcgctgcgct agctggagtc ttgccgttac aggcagcaca ttttggttac
    13921 ttttcctttt atactatacc actcaactta ctttgcgttc ctttagttgg cctcgtagta
    13981 atgctggggg tggccggtat cgtaagcggt ttagttagtt ctgcattggc cagcatcttt
    14041 ttcacgatga cttggcctct gctggcgttg ttggcccgct tgcctcgttt tagtatgcgt
    14101 ttgccggcag ctttcctcta cctgccagca gtacaccccc tttggtggtg tatttatgca
    14161 gctgtactgc tcttgattat cgccaataaa agctggtcgt taacagctag aagaatattg
    14221 tttgtgctgt tgttaggcaa cttggcctgt ttgtctagtt ttactggcag caggtaccgc
    14281 cagttggagg taaccttgct tgatgtcggt cagggactgg ccgtacatat ttgcaccccg
    14341 gcagggcagc acctgctagt agacgctggt ggtggtcaat ttgcgacagg ggagcagatc
    14401 gtgctgccct atttaagggc aaagcgagta cccaagttag cagcactggt actgacccat
    14461 ccccacgacg atcattatgg gggtatgcag gcggtcttag ccgccttacc cacaaacgtt
    14521 tatctgagta atggtgagca ggaggattcg gcatcatttt ctgacttgct cctgacgctg
    14581 caggaaatgg ctattcctca gtatcgtgtt agtgcgggtt atcggctgct tattgatggc
    14641 gtcgagttga aaattctttc accgccggca aaacgttttc tcaatactag tgacgatgca
    14701 aacaataact cgttggtgat cagccttagt tatggtgact ttaacttact gctgacgggt
    14761 gatgccgaag cagccgccct cacagagctt ttacagacag ccaaaggcaa actgcaggct
    14821 gctgtgctgc aggtgccaca ccatggcagc cgcaatgcgc tctcgcctgc tttgctgcgt
    14881 gaggcagcag cgccggtggc ggttattgct gttggcaata accctttcgg ccaccctcat
    14941 gctgaaactt tggcgctgct cgctgaacaa aaaatagatg tctaccgtac tgatttacat
    15001 ggtgccgtaa ctatcactag cgatggcaaa aaatggcaga taactcccta tctctatccc
    15061 tgacggcgcc tcacaactgt ttcatagatt agtctgtctt ggatataata acgagaagaa
    15121 gcgagcaatg tctgggggga tttttttgcg cgtcttaatg ttttcctggg agtacccacc
    15181 acgggtagtc ggtggtctgg ctcgtcacgt cgctgaatta agcgtggcgc tgcaggcaga
    15241 aggtacacag gttgatgtga ttactattga tgaggatggc acagcgccag aagtagaaat
    15301 aattcaaggg gtgcatgttt ggcgagtacg tccctatcct cttgtgccgc gagattttat
    15361 ctctagtgtt ttgcagtaca atttagccat gttggagaaa actcttgctt tattgatgca
    15421 gggtgaagat tatgacctga tccatgctca tgactggtta gcagcttatg ccgcacgagc
    15481 tgttaaacat gccttaaaaa agccgcttgt cgctactatt catgccacgg agtatggacg
    15541 caatcgtggc ttacatacgg aagagcagcg ctatataagt gatgtggaat ggtggctcac
    15601 ctatgaggct tggcaggtga tctgctgcag ccaatatatg catgcggaat tacaaagagt
    15661 gtttcatttg cccgatgata aaattgttcc catccctaat ggtgtaaatc ctcgtcagtt
    15721 tcagacagat gctctggaag cagcggcacg ggcacgctat gcggctgccg atgaaaaaat
    15781 cgtctttttt attggccgtt tagtgcagga aaaaggggtg cagatcttat tagaagcggt
    15841 accccatatt ttagcggttg taccaaaaac aaagtttgtt attgcgggca aagggcccgc
    15901 ttgggatgat ttgcatctgc aggctagaaa aatggggatt gaacaacatt tatattttac
    15961 cggttatatt gatgatgtga cacgcaataa actgtataaa gcggcggcaa ctgctgtctt
    16021 ccctagtctt tatgagccct ttggcattgt ggctctagaa gcaatggcgg cctctgtacc
    16081 cgttgtggtc tcggatacag gggggctggc agatatcgtc gaacatggcg ttactggttt
    16141 gaaatgttat cctggccacg ctctttcgct ggcagaacaa atcattactc tcctgcagaa
    16201 gcccagcttt gcccaacaat tagcggctaa agcaatgcaa aaggtagaaa gcaagtatgc
    16261 ctggcaggaa attgccagac aaacccgcaa catctaccaa aaggtcttag ctgattatca
    16321 acaaagtgat tggccgacac aacagctttt gacgcagcca aaaactgttt taccccaatg
    16381 cgcacaataa ccttaacaac ttaagctcaa ggggggatga aaatgaaaac aatcatcatg
    16441 gccggtggtc aaggctcacg tttacggccg ctgacttgtg accgacctaa accgatggtg
    16501 ccgctgatta accggccgat gatggaacat atagtggcct tactaaagcg gtatggctta
    16561 cgcaaaattg gcgtgacatt acaatattta ccgcagcaaa ttaaaaacta ttttacagac
    16621 ggccgggaat tcggagtagc gatgcgctac tttacggaaa attccccgtt aggcactgcg
    16681 ggcagtgtga agaatgctgc ttcttttttg gatgagactt ttatggtgat tagtggtgat
    16741 gcgctcacag actttgatct gcagcaagca attaactttc atcgacaaaa gggcagcctg
    16801 gctacactgg tgcttactgc cgtggaacaa ccgctagagt atggtgtggt cattaccaca
    16861 gaggagggac agatcacaca gtttctggag aaaccgagct ggggtgaggt ttttagtgat
    16921 accgtcaaca cgggtattta tattttagag cctgaaatac ttgcttttat tcctgatact
    16981 gtgaaattcg acttttcgca agatctattt ccgctgctga tggccaaggg gtatcctttg
    17041 tatggctatg tagcaaaggg ctattggtgt gatatcggca atttacagca gtatcatcaa
    17101 gcccatttag atatcttgca gggtaaggtg aatattgagc tgcaggctag agaaatttct
    17161 ccaggtattt atgtcggtga ggatgtcatt attgagccgg gtgcctacct cactgccccc
    17221 gtcttgatag gcaatggtgc ctgtattaag caaggcgcag ttgtggcgga gtcctccgtt
    17281 attggtccac aaacacaagt agattcattt gcctctgtca agcgcggtat tacttggcgc
    17341 aatacatacc ttgctccgca gacagaaatt cgtggtgcca ttctcggcaa tcgggtgcag
    17401 gtactgcaag gcacctcatt attcgagggt gctgtcgtag gtgacgacac tgttttagaa
    17461 gaacattgca ccattaaacc caatattaaa atctggcccc ataaacggat cgccagcggc
    17521 agtacggtgt ccgaaagcat tatctggggc acaaagccca cgaggaacct ttttggcagc
    17581 gctggcatca gcggtgaagt aaatttagag attacaccgg agctggtggc gaaacttgcc
    17641 gcggcctatg gttccctctt gggtgaaggc cgccaagtcg taatcagtag tgatgactgg
    17701 aaagcatccc ggatgttaaa ggctgccgca ctggccggac tgctttcagc cggtgtgcaa
    17761 gtttatgatt tgggtgtttc tgtaaccccc attactcgcc aggcgattaa gcagttagag
    17821 gtgcaaggcg gtatccatgt gcagttgtca tacgatgtga ataccaacac acgtctgcaa
    17881 ttttttgatg cagcaggctt ggcactaacc aaagcttggg aacgaaaaat tgagcagact
    17941 tactaccgag aggactttaa acggatcaaa ggtttagctg tgggtgaaag taatcagctt
    18001 gcacagtatg cagattccta ccgggaaaat ttgctgcaga atttagatat tactgttttt
    18061 gcccgtaagc gccgcaaagt actcttagcc tatgtgaccc cttggttgta cacttatctt
    18121 ttgcccatcc tgcagcagtt aaattgtgaa gttatttcta tctctgctcc tgaggacggg
    18181 ccgttgacgc tgcaagctct ccaggagcaa tttagtagtg tggctgctac cgtgcagcgc
    18241 ttaaatgccg atttaggtgt ggtgatggat gctaatgcag aaaaattggt gctctttaca
    18301 ccacaagggc aaattattgc cgaggagctg tttagagcgt taatcaccac gatcattttt
    18361 cgtagtgcca gcggcagtac gatggctgtc cctgtaactg ccccggcagt aatcgaaaaa
    18421 ttagctgcta tgtaccaggg caaggtctta cggaccaaaa caacaccgcg ttctctgatg
    18481 gaagctttgg ctgccggtga taactgcagc caaagatatg accccttcac gctctctttt
    18541 gatgctatct cctccttagt aaaaatactg gaatatttag cggctgaggc tacagacttg
    18601 acgacacttg tagacagtat tccgccattt tatgtgcatc agcgtgaagt tgactgttcc
    18661 tggggggcaa aaggtgctgt catgcgccgt ctgattgagg agacacgcca tgcagatgta
    18721 gagctgttag atggagttaa agtgcagcat cagcaggggt gggctttggt cctgcctgac
    18781 tcagaaaaac ctgtttatcg tatctacagt gaaggttata atgccgaaat agcagaagag
    18841 ttaactgatc tttacgcgca aagaattaaa aagtaccaaa ctgaattagg taaacagaga
    18901 aacgagtcct gacaaaagtc agggctcgtt tctgtatagg ttaaggagct taattaaata
    18961 ataataaata gtgtgagatt ttttattgta gataaaacgt tgggcaaaaa ggggtggcaa
    19021 ggtggataaa gttgttagcg gcggtatagc aggggggatt gctggcctac tagttggtct
    19081 gctttctaaa atcttgagta aagtaaagct gtgccaacta tgtttaatta aaattggcgg
    19141 gggactgtct aaccaagcga tgttagctga gaatagtgct ttgttgtggc aagttattgg
    19201 ttggctcagc catctgctta ttagtatggt tttaggcatt attttcttgt atctgctcca
    19261 ttacacgggt aaagagtaca tcctgcttaa aggagtgatg tttggcgcag tcgtttggtt
    19321 agttgatatc accattattt ctcccctttt aggttttatt cccgctcgca gcaggccgct
    19381 tgacctgtta atcctgctgg cttaccacat cattttcggc tatctggcag ccgtattgag
    19441 caggcgcttt gccaaaacaa cagcccaagc gtagagcttg ggctgttgtt ttggctcact
    19501 aaaaatacct tgtccttact gttacgagtt tagggaggcc ataacttttt ctgtttgctg
    19561 cagaggtgca taaatgcgga aatctagctg agaaaagata ttatctttgg tttgtagttt
    19621 tcgcagccaa tcctcatcga tcttgttttg ataaatttgc tgccacagac ggttaaaacg
    19681 cactaaatga tcatgggcac tttcctgtgc atactgcacc atggtaccgg tggtcataat
    19741 aaacggccaa tcactggctt gtgctaacag cagttcacga gccagttgat ttaaagcttc
    19801 ttcctgcagc ccactggcgt gggggaaatc aacagctgct tggctcatgc gttctgctgc
    19861 catatgtaga tgacgataaa gccaatcatt gttttcgttt aaccagactt catgataacc
    19921 attactaccc cagctggagg atgaaggctc tgcttcctgc agcggtagac ccagacttaa
    19981 atattctgag ggagtaatgg gacgaaatac ctcttggtcg taatctattt ttctaaataa
    20041 atcttctagc caggcaggcc cttcatacca ccagtgacca aacagttcag catcatatgg
    20101 tgctaccaca atggggggac gatcaatctc accggcgaca tattctattt ggcgttcgcg
    20161 gttaaacatg aaattgcctg cgtgttgtgc taattttttt tgtgcttgtg ccaaattata
    20221 tggctcttta gcatcggtct tgcccgtaat acgataatac ttaaaacctg tatgggtgcg
    20281 aatgccatcg ggatgaatgt aaggtccaat ataatctagt ggtaagtcga aaccgatatc
    20341 acgataaaaa tcacggtaag caaaatcgcc agggtaacct tctgttttac tccaaacctg
    20401 cttgcttgat tctacatcgc gaccaaaagc agcaaccccg tgaggcgtaa gcaagggact
    20461 ataggtgcca taagcaggtc taggtttggc aaagacaatg ccgtgagtat cgacaaggaa
    20521 gtagttgatg cccagctctg ctagaagagc atcaatcttg taatcgtagg cacattccgg
    20581 cagccagatg cctttagggg cctgaccaaa cagctgtgta tgttgttcaa cggcagtaaa
    20641 tagttgtgca tacactgctt ccgggttcgt aagtaaaagg ggtaaatagc catgagttgc
    20701 cgcacaagtt ataatttcca gtttgccgcc agcagctagc tgtcggaaag cacccgctaa
    20761 atctgcccgg taggttgtaa aaaaggtatc atacgcagac tgcaggcgct gcaggtaatg
    20821 gccggccaaa ggggagaaaa caggatcgtt ggcggtacgt ttaacttctt tatcggctaa
    20881 ctctagtaag gacagcaagt agtgacgata tttcccttgc agatattcat cctgccacat
    20941 ggcagccaag gttggtgaaa tggaaatggt cagccgataa tcaatcttat cctgttcaag
    21001 ccgggtcaag ctttccagca aaggtaagta tgtttctgta agcgcctcaa aaagccattt
    21061 ttcttcaaaa gagtaggtat attcgggatg ccggacataa ggcagatgag catgtaaaac
    21121 aagcgagaaa taacctttcg tcaccatggt cctccttctg taataggctt gtcgcaaatt
    21181 agttcataag atgaataacc cgacatgctg cgatagtaac ggtgctgcca aaaagcaaac
    21241 attcgccagc gcggatcgag gactgaagaa atggaatcac gtggtgtgtt tacagcgttt
    21301 gaggtcagta tgtttacaaa gctgccgtca gcaagcagac gacctaactg cgcataatag
    21361 cgttgatcgg catcgtttac cgctaagtac cagttgctgg caaggttgtt gataggaaaa
    21421 tcctgcacct tcgctgtact gacgtttgtt agtttaatca tgctggagcc ccttgtccac
    21481 agttcagggt attgctgctc atacttttgt tctagttcag gtgtaacctc ccaataagct
    21541 aaaagcttgt gcggatctcg tggcaaaagt accaggcggt ccacaccgta gctgttggga
    21601 agtgtccaac ccgcatttaa ttcatgacga tccggtgcgt gcataactgc ctccttacaa
    21661 gcaaaaaatt ttagtaggat agatgaagaa catgtaatag tattgccctt gtctattgtt
    21721 cttatgtttg cgaaattccc aagctgccag actttgcata taaatggcgc catgtggcaa
    21781 gctatgcgta catcgcacaa ggggctaata atgctggctg gggggagata atggtgagaa
    21841 ttgccttgat ctgccgtaat ttaactatct ttgacacaga cgcaacactt ttacaacttg
    21901 ctcaagaaat tactcagcaa ggtcgggaat gttgtatttt tactgcagac aaagaactta
    21961 aagtgcaaaa accagtctta tttgcttctt tttatgtcgt cagcggcggg cagctccagc
    22021 agattgcagt gacagaacaa agacagggtg ccctgcgcat ttttactatt tctgctgcca
    22081 gctctacgct ttttgctgct gctgtggccg gcatggcccg gcacctgctg ctgagcgaag
    22141 aaaaaataga tttgctgcat cttttcagtg ccgcagatgt ttgcgcggct ttactatgtc
    22201 agcaatattt tcaaattcct tttgtctatt ctgtccgaga acttcaagaa ataaaacaaa
    22261 gaaagcaaga ttttcaggat ttaagcctga cggccccagc acaagttttc actgttggtg
    22321 actttatttt aaatgagcaa ttagcagcgc aggaggcagc ttacatgatt gatgaaactg
    22381 gtaatcctcg cttatggcgt gaattttttt gtgcttatgc cggcaaatat attgaaggta
    22441 aaccgtttat caatgtgagc tattggtcac agttaaaatc aacgccgcgt caaactcgta
    22501 aaagtgaact tttacagaag ctgttaagtg aaaaggattt cgtagttgcc tgtgaaaagc
    22561 cgcctgtaca gccacctgta cagccacctg ctctaatggg ggaggtgctg tttgtcagct
    22621 ctaatcttaa caagacagaa cgtttaagcc tgctgcaagc cgcagactac tatttagtag
    22681 aaaacctaca gcctgaaaat ctcttaacgg cgatggccgc tggatgtatt cctattgctc
    22741 ctgtccagag cttcacgcaa agtttaatac atgacgagga gcagcagtct gcgcaggcta
    22801 ctggatattt atatcagcaa gaaggttggc caagtgtttt acagcgagcc tgcactgatt
    22861 atcgtgcgcg gcctggtcag cagcagcagt tacgtcaacg cggcatgatg ttaattcgtg
    22921 actattttag tattaagacg gcggccgcac tttatgaacg gatctaccat ggttctgcac
    22981 cggttagatt gccctttatt atggaaaaca acaggcagcc aactgctact ggcgtaaatg
    23041 aggtacaact atgaagaaaa cagcaggccg caaaataaac tttattttag ctttacataa
    23101 ccatcaacca gaaggcaact ttcctgacgt cttcgaaaaa aactatcaat tagcgtatga
    23161 accctttatc caggagctgg aacaattccc tgaactgaag gtggtgcagc actattcggg
    23221 cattctttta cgctggctgc aggagaatca tcccaacttt atgtcccgct tgcgtcaact
    23281 tgctgccaac ggtcaagtag aactgatggg cggtgctttt tatgagccaa ttttggtcat
    23341 gattccagat gaggataaag taaaccagat taaaaagcaa agccgttttc tccgtgaaaa
    23401 cttccaagtt actgtaagtg gtgtttggtt ggcggagcgg gtctgggaac ctcatttggt
    23461 gcggcctctg gctgaggcgg ggatgcatta tactgttttg gatgatgagc tgtttgaaaa
    23521 agttggtctt aaggaagaag aactgctgcg tgcttatgtc gtagaagagg cgggtagacg
    23581 cctggcggtc tttcccatta acgaaaagat gcgttatcta attccttttg ctgaacctga
    23641 agaagctatt gcttatttag cagaaatggc cacacctgaa ggcgagcgtg ttgtcgtcct
    23701 ggctgatgat ggtgaaaaat tcggttcttg gccgggaact ggcaaacggg tttatgaaca
    23761 aagatggctg cagcgatttt ttaagctgtt aaaggaaaat agtgactggc tgcaggttac
    23821 tacctttacc gagtatctgc aaaactacca cccagctggg ctggtttatc tgtcggcagg
    23881 ttcctaccgc gagatgatgg aatggtcagg tggattttgg cgtaatttcc tggtacggta
    23941 tccggagagt aatcagattc ataaaaaaat gctgcatgtg cgcgaaaaac tgcagagctt
    24001 acccgctagc ccccaaaaaa gagaagcaga agagtatctc tgggctggtc agtgtaactg
    24061 tgcttactgg catggcgtgt ttggcggact ttatctaaac tttctacgtt ctgccctgta
    24121 tagtcgtctc ctcgctgctg aagagattac agatcgtgcc ctacatggaa gcgagccatg
    24181 ggtagagtta gaaagaaaag attttaacta cgatggtgcc gatgaagtgg tagttacggg
    24241 atcggacttg gcctttattg tcgctccgga acaaggaggc gctctcttag agcttgactt
    24301 taagcctaaa cgctttaatt tgtttgatgt gctcacgcgc agcaaagaaa actatcattg
    24361 cagcctttat gagcaagcgc aagaaatatg caagactgat gaggttaaaa ctattcacca
    24421 catttcccgg gttaaggagg caggactgga gcagctatta acctatgacc cttatcgccg
    24481 tgcttcttgg cgtgaccact ttttcccaca gcaaagtgat cttttacaag caattgctga
    24541 agtggaaata ggtgattttg ccaatgcccc ctacgagatt atttctgcca cagagagcaa
    24601 tgctgcgcat ctggaactgg aacgccatgg tactgtagta ttggcaggtg aagtatgccc
    24661 ccttcttgtc tataaagcac ttcactatca gccgcaggaa ggtgagcttc gttacagtta
    24721 tcgattgcaa aacaggggta aaaaagagct tgagcttgac tttgccgtgg aatttaatat
    24781 taatttcttg gcaggccaag cttcagaccg ctactatagt gcaccggggc agatcttgac
    24841 caatcgccag ctgaattctc gcggtagaac agcaaacctc actcgttttg gcctggttga
    24901 tgaatggcaa ggcatctcta ccatgtttcg ttttgatcga ccagctacgg tatggcggat
    24961 gccgattgaa acagtctcgc agtcagaaga aggcatggag cgtgtttatc agggctccat
    25021 gatcctgcca acttggaagg ttaagttatt accgcacaca ccttggtcac ttacctttac
    25081 acaatcggtc tcagaactgg tgcgggaggt gcataaatga aacctttata tcttgctttt
    25141 atttggaatc aacatcaacc cttttatcag gatacagtta aaaaagaata tattatgcct
    25201 tgggttagat tgcatgccac caaggattat tatcaaatgg ctgccatttt acggcactat
    25261 ccaaccatcc ggcaaacctt taacttaacg ccttctttgg tggcccagct ggaggattac
    25321 cttaagggtg cagatgatta ctacttacgg gtgatgaaac cggcagctgc tttatctgtg
    25381 gcagaaaaaa ggtttctcct gcagcattat tttgacattc actgggagcg ggtcattgcc
    25441 aagtggccgc gctatcaaga attattagct aaacagggcc actgtaagga gccggcggca
    25501 gtcgaggagg ctttagctag ttatacggaa caagattatc ttgatttaca agtttggttt
    25561 aacctcgtgt ggattgatcc agaagtacgg gaagaggatg cagaattacg agccctgcaa
    25621 actaaaggca ggtattttac ggaagcagat aaacggctag tcttgcagaa gcaatgggag
    25681 ttaatgcggc aagtggtacc tgtacatcgg caattggcag agaaaggcca gattgaatta
    25741 atgaccacac catattatca tcccatcatg cctttactca ttgatacacg cagtgccctg
    25801 cgcgcttctc caggtttacg gttaccaaag acttttagct atccggaaga tgccgccgaa
    25861 caaatcatgc gcgccattaa tcaataccga ttaatttttg ctggctggcc gcaaggagtt
    25921 tggccgccag aacaagctgt cagtcctgag acactagcct tgtttgcagc atccggtttt
    25981 agctggacag taagtgatga agatatttta gcccgttccc tgcagattga attttaccgc
    26041 gactgctctg gtcatgtact gaatgcggat caattgtatc gtccctattt agtaaaagtg
    26101 ggcagccggg agattgccat ggtctttcgt gatcatcatc tttctgatcg ccttggtttt
    26161 gtctaccatc aaatgaatat tgaccaggca gttgatgatt tagtgcaccg tttacataag
    26221 ataagagaag cagttagcga ttgtgagggg ccgcatctag tcaccatcgc cctagacggt
    26281 gagaatgcct gggaatggta tgataatgat aaaaatgagt ttttacataa actttatagc
    26341 cgtttgtcac aagactcctt gctgcagacg gttacgatct ctgaattttt gcgtgattat
    26401 cccccgcaac gtgttctgcc ccaactctat accggctctt gggtcgatca taatctgact
    26461 cgttggattg gtagtgagag taagaaccag ttgtggcagc tgttgctgga cactcgtcgg
    26521 atgctggaag aagtacgggg taccattacg gcggaacaac tgatgaaagc taaagaaaat
    26581 ttgctaattg ccgagggcag tgactatcct tggtgggttg acagcatgcc ctattatctg
    26641 tcagcaccct ttgaagcact tttccggaaa cacctgatta atgtctatcg tgagtgtggg
    26701 caagaggtgc caccagcttt gctgcgcact gtgctgtgtc ccaaagatgg tgaagcggtc
    26761 tggtctgaag atgatcctct ggcaggcccc acaaccatgg tcccgacgat aaaaaactaa
    26821 gatctgcagg caggcaggtc tcaaagaagg aaacattctg gccggcgtgg aagtaaacag
    26881 tagaacattt agactgggtg atacagatgc aaagacgccg gtacgaaata gatgccctgc
    26941 ggctggaacg agaaattaag gcgggcgaaa gaaggcccgt ctatctcttt tatggtgagg
    27001 aagacttttt acaatcagca gctgtcagta ctgtggcaga ctatttactt ccaggagggc
    27061 tacaggacag cagctgcact cgcgctgccg gttcagacta ctcactggag gaagttttgg
    27121 acatggtgga cacagtagct ctttttagcc agggtcggct gctgattgtg caggctgcac
    27181 cgtatttcat caaaggcagc cgtttagagg atgccgcagt gcagcgttta ctggcctatc
    27241 accagcaaaa aagttcagac tccacgatca ttttctgtac agcagaaaag gtatcagcag
    27301 cgaaagtggt gaaagaatta acctctgctg gtgctgttta ccacttcgaa cctctgaagt
    27361 ggacgccgtt aatgacttgg ctaagggagc gggtagcggt taaaggcaag aaagcgacca
    27421 caccagtgct gcagttcttg atcaaccgtg taggtcagga tttacgcaga cttgcttcag
    27481 aattagataa actggtcagc tatctaggcg agcagcaaac aatagatgag cagagtatcc
    27541 ttgctgttac ttcacctggg gcgcaagcaa atatcttcgc cttaatcgat gccgctgtct
    27601 atgggagaac agcattcgcg ctgtccttat taaaaaatct tttagtcctg cgggaaccac
    27661 cactgcgtat tttaagtatg ttgacacgac aattccgcct cctaggggaa gcaaaagagc
    27721 tgttggcagc aggagaagat aatatagcta aggccctctc tttgcactca tttgctgcag
    27781 agaacttaat gaaacaggtt cgctgtacgg atgaggctcg cctcaaccgt gcggtggaac
    27841 tattactgcg ttgtgagttg gatattaaac gtggccgtat tcaaccagtg ctagctctgg
    27901 aaacattaat agtggcttta ggggtagatg cacctggccg ttaattccca cctctcataa
    27961 aatttacata aaaaaaagac gcacccacta aatagtggcg tgcgtctttt ttcgtactaa
    28021 aatgtaagga acacattgag agctccgaaa aacaaaaaac ccggaaaggc cgggcttctg
    28081 ttactgttac ttattcagct gctgcttgtt gagctgcagc tgctttgttt aaatgcttag
    28141 tcatacgtga ttttttacga gcggctgcgt ttttatgaac aacgcctttg ctcacggctt
    28201 tatcaagtgt ttttacagct gcctgtaatt tatcaccagc ctgatctagg ttaccttcgg
    28261 ctagtgtgtc tctaaaccca cgaacagaag ttttaaccgt acttttcaca taacggttgc
    28321 gagctgtccg tttggcggta actctcatac gctttcctgc ttgcttacta ttgggcaatg
    28381 cgttcacctc cttcgaaact tgctacgagg acacaacaat tctaccatgt gtttctttaa
    28441 aatgcaactg catgtcaaga tgtatagcaa aaaaacgttt ggttaagcta gtagcagtta
    28501 aataaacaaa atggaggtga attatgtgct tggtttaatt gtacgcttta ttgtgtcagc
    28561 tattgttttg atgttggtaa gcttcttgtt gcctggtttt gctaatatgg gtttcgctgt
    28621 agccttggta gcagcgattg tcattgcctt gcttggtttt atcgtagaaa gtgtacttgg
    28681 taaatcaatt tctccccaaa accgtggtat tgtaggtttc cttacttcag ccattgttat
    28741 ttatctggca caataccttg tctctggcat ggaagtgacc attcttggcg cggcattggc
    28801 agccctggtt attggcatca ttgacgcctt tgtaccgacg gaattacgct aggaggagtc
    28861 ccgtgaccct gaaacaacaa aatcgcagta tccgtactga tttggccatt gaagcgcacc
    28921 aaatgttggt gggtgaggtc gaacaagaga tccccggtgt actagtggaa actgaagaaa
    28981 aggatcaaat tcgggtttct cgagtccata ttacttctcc agaagcggag gagcggatgg
    29041 gtaaacagca gggtaagtat attactttgg aagcaccggg actacggcaa aagaatacag
    29101 aactgcagga aatggtctca cgtcaattta ccaaagaact gttaagcctg gttgaacttg
    29161 aagacaaggc cacaatcctc attgtcggtc ttggtaactg gaatgttacc cctgatgcac
    29221 tgggtcccag agttattaaa gacttgctgg ttacacgaca tattttggct ttacaaccgg
    29281 aaacacttgg tgatgggttc cgtgcagtga gcgctatttc tccaggtgtt ttaggcatca
    29341 ctggggtaga gactggtgag attattcaag gattggtgga aaaagtaaaa cctgatctgg
    29401 taattgcagt agatgcttta gctgcgcgca gtctgagtcg tttacatacg accattcaga
    29461 tagctgacac cgggattacc cctggatcgg gcgtgggcaa taaacggtta aaaatttctc
    29521 acgaaactat tgggattcca gtcattgctg tgggagtacc cacagtagta gatgcagtca
    29581 ccattgctga tgatttaacc agccatttgc tcgaggctct aatgcaggaa gcaggctctg
    29641 attctccagt gtataaagtg atacaaagta ttgccgaagg tgatcgacga gccgtaatgt
    29701 cagaagtact agatccttat ggtgggcgct taatggttac ccccaaagaa attgacacac
    29761 tggtagagga tatagccaga ttactggccg gcggcattaa tgcagccatg catcctgcaa
    29821 ttcaaagtga agaagtaagt aagtatctac actaaacaaa aacagtagcc ctagggagtg
    29881 tttgcagaac tacccaacag tagctgcaat actcccattt ttgtttaaag catgattgct
    29941 agacataaat tagcgtttag agcatagctt tatatatagg ttagctaagc gatgttttgt
    30001 cgaggaggga tggccgtggc cagaagaata catttttttg ttcgccccaa tagacgcttc
    30061 aggtcaagta agatgggcag gggtaaggtt tgggtagctg cattggccgc ggtagtgctg
    30121 ttgatggggg gaagttatct ttatcgtcag cagcttgaag ctgttgctac ttttgccgca
    30181 accccgcagc aagaatcttc tccgctttta agttttggca aatcactatc tttgcgcata
    30241 gtctcaactg ttatccctgg atttaatgat catgaccacg cgctaagtga gggtgaggtc
    30301 agcactgccg aagaccatta tttacagaaa ctagcactaa atgacttgcg taatcccaaa
    30361 aatatgattt acgcccagat tccttattta ggggtggtgc cggaaacact cattaataag
    30421 cctgctaccg atcaacctga gacaccaaaa attacctttc ctgtacgagc tgaattaacc
    30481 ggtgccggaa cagtcttgct ttatcataca catgcgacag aatcgtttgt tccttcttct
    30541 ggtaaggctt ttgtttcgac tgatttgtct caaacgatta cgcagctcgg cgccgaacta
    30601 gctagtttgc tgcagaatga ataccaaatt tctgtctacc atgaccaaaa gatccacgat
    30661 gtgccgcgta ctggtgcata tgaaagggca ctgcccacga taacagaatt attaaaaacc
    30721 taccccaaca cggcgttggt cgtagattta catcgtgatg gggtagctaa aaaaattact
    30781 actactacgc taaatggtca agcggcggcg cgtatcttgt tggtggtggg gtcacgccat
    30841 gacgattggc aggaaaataa ggcaaaagct gaatttatgc atcacacgct ggagaaatta
    30901 gcgccaggaa tttcgcgtgg tattcgtgaa cgccccttag tttataatca gaatgtgcat
    30961 gctggttgtc tgttgattga gttaggcggt taccagaact ctttagaaga agtgcggcgg
    31021 acactgcccg tcttggcaga agctttagca ctcctgtaca gttctggcca agggcagtaa
    31081 gctgctgccg ctattatctg ctggtcggca ggtataaaag aataggtccc tgggagaaaa
    31141 tatagtcaat gataatcgtt ggaggggacg ggcatgaaaa agcaaacggt gctgtccgta
    31201 ttgcttattt ttttatttgc ttttgcgtta ttctatgcag gtttaagttt atcagagcag
    31261 gctttgcagc aagttagcgg gacggtgagg gagccggcag cgctttctct aagtcgtgat
    31321 gaagccggaa cttgggtgct gacttttgcc gggcgtacgc tgcgcctgac taggcagttg
    31381 tggcaggtgt tttggcgaaa gaacggataa tgtctgtact gttgcttgtc caagggctaa
    31441 aatgctataa tatgcaggta gatgcaggag gatgacaatg caacagaaaa acatacgcaa
    31501 ttttagtatt atcgctcata ttgatcatgg caaatcaacg ctggcagacc gtttgctgga
    31561 agctaccggg gcactttcgc agcgggaaat gtctgaacag gttttggatc agctggattt
    31621 agaacgtgaa cggggcatta ccataaagct gcaggcagtg cgtttgatgt atcaagcgca
    31681 ggatgggcag gaatatatgc ttaatctgat tgatactccg gggcatgttg attttactta
    31741 tgaagtatcc cgcagtttgg ccgcttgtga aggcgcatta ttggtcatcg atgctgctca
    31801 gggcatcgaa gcgcaaaccc ttgccaatgt ctatttggct ttagagcata atttagagat
    31861 aatcccggtt atcaataaaa ttgacttgcc tagtgccgat gtggaaaaag caaaacgaga
    31921 gctggaggaa gttatcgggc tgtccggaga tgaggccgtg ctgacgtccg ccaaaactgg
    31981 acttggcatt acagaagttt tggaagccgt agtgcaaaga gtgcccgctc ctcaaggaga
    32041 gcgtgcggct cccttacggg ctttaatttt tgattcacat tacgattctt atcgtggggc
    32101 gattgcttat gtacgcatca cagaaggcac gcttactgag cggatgaaga ttaaaatgat
    32161 gtctaacggc aaagtttttg aggtttcaga gattggcagc tttacaccgc acatgacgcg
    32221 tacaggcaat ctggctgccg gtgaagtagg ttatgtggtg gcaggtgtta aaaatgttaa
    32281 ggacactcgt gtcggtgata cgattactgt cgcggataac cctgctgttt caccgctgcc
    32341 aggttatcag aaggctaacc ccatggttta ctgcggcttt tatccggcag atagtaatga
    32401 ctatgtagat ttacgcgatg ctctggagaa gcttaattta aatgatgcgg ccttaacctt
    32461 cgagccggaa acatcggaag cgctaggctt tggttttcgt tgtgggtttt tagggctatt
    32521 gcatatggag attattcaag agcgcctgga acgcgagtac aaaatatctt taattaccac
    32581 ggcacctaac gttgtttatc atgctaccct aaataacggc gagctgctcc aaattgataa
    32641 tccgacacgc atgccccaag cagcggagct tgcctctgtc gctgaaccgt ttgtttctgc
    32701 ccgggtaatc atgcctaacg attatgttgg tgctgtaatg gaactatgtc aggaaaaacg
    32761 gggcatcttt atcaacatgg agtatcttga cactacccgt gtaaccctaa tttatgaaat
    32821 gcccttaagt gaaattgtct ttgatttttt tgatttatta aaatcacgta ctcgcggcta
    32881 tgcctccttt gattatgaat tagtgggcta ccgtgagtct aaactagtga aagtagatat
    32941 tctgcttaat ggagcagccg tagatgcgct ctcctttatt gcccatctgg agcgggctac
    33001 caaacggggg cgggaactgg caagtaaatt acgggaacta attccccgac aaatgtttga
    33061 agtgcctatt caggcagcta taggacaaaa ggttattgcg cgggagacga ttaaagccat
    33121 gcgaaagaat gtgcttgata aatgttatgg cggcgatatc acacgtaaaa ggaaattact
    33181 agaaaagcaa aaagcaggta aaaaacgcat gaagcaggtt ggaaatgtgg aaatcccaca
    33241 agaagcgttt atggccgtgt tgagtatcga ttaatgatgg gtacagctgt ttaccttcac
    33301 atcccctttt gtcgtgccaa atgccattac tgtgacttcc tctcctatgc ttgctcaacc
    33361 agcggcagct ttactcccca actctatctg cagggcttgg ttcgtgagtt ggctatccgc
    33421 ggcgcggagc tgatctctgc tggccgggag gtcacaactc tttatcttgg cggcggcacg
    33481 cctactgtct tagagccacc ggacatggca gaactgttaa gctgctgcca caggtattta
    33541 cctttagcct cccctgaatg gacagtagag gctaatcccg gcaccataac gcccgcagca
    33601 gtgagacagt tagcaaagct tggcgttaac cggatcagcc ttggggtgca ggatctttgt
    33661 gatcagcgtt tggctagtct gggacgcttg cataatgccc aacaagcgcg gcaggctttt
    33721 ttgctgtgtc gggattattt tccctctgtt tcactggatt tgatggccgc tttacctgga
    33781 caaacctcgg cacagctgtt ggagacatta gctgccgtct gtgattgggg tccgcagcat
    33841 ctgtctgttt atggtttaaa agtggaagag ggaacacttt tggccgccca ggtagcagca
    33901 ggtcaagtcc agctgcctga tgaagatgaa gtactgacga tgtttcttgc cggccggcaa
    33961 tatttgcagt cacgcggcta tgaacattat gaaatagcga actatgcccg gcccggccat
    34021 caatgccgtc ataatttaac ttattggtct aaccagccct acttaggctt gggtttaggt
    34081 gctcattcgt attggcaagg tgtacgatgg gaaaatacgc gagatccaga gacttatttg
    34141 actcagcttg ccgcaggtca gcttccccag gctgtacaaa ctactgtgac gaaagatgag
    34201 gctatggaag ataccatgat gcttggttta cgattgctga acggggtaag ttttgcagat
    34261 tttcaggaaa agtttggtca ggatctacgt caggttttta atcaggagat caagtatttg
    34321 cagtcattag gggctgtgga atgtgatgaa cactatatcc gcttgagtga aaagggtctg
    34381 ccagtggcta atgtggtgtt cgcagagttt ttgcgttagt cttttttgcc gagtatatac
    34441 cttgacaaat accagtgata atggtatttt tttattagat tagcactcac cttaagagag
    34501 tgctaatcta aggaggcatc aaaatgactg gtttaaatga gagaaaacaa aagattctcc
    34561 atgccattgt cacagactac atccaaacgg cagaaccggt cggctctcgt gcactttcac
    34621 gccattatca aattaaacta tcggcagcca ccattcgcaa tgagatggcc gacctggaag
    34681 agttaggttt tgtgacacag ccccacactt cagcggggcg tattcccacg caacaaggtt
    34741 atcgtttttt tgtggacacc ttgatggatc aggtagcttt gactaaccca gaagaaaaat
    34801 atcttgcaga attctaccgg caagtacaaa aaatgcagga aattgatctg attatccagc
    34861 acacggccaa agttttatct gtgatgacta gctacacttc actaattctt ggaccgctgt
    34921 ttagacgcag tgcctttaag cagatgcgca ttattcccat tgatcaagag cgggccctgg
    34981 tggttttaat taccgacacg ggttatatta aaaacaaagt gattactttg cctcgtgcct
    35041 taaatcagga agaattaaac caggtgatag cctatctgaa tcaacagtta gcgggcaaaa
    35101 caattgctag tttatcggca gagcacctgc gtcaattacg gcatgatctt tatgctaggg
    35161 tagatatttt aaatcatatg atgaatatgc tggaagaaag tgcggcagag ggagaaagcc
    35221 gtgtcttctt agggggtacc tccaatatcc ttaaccaacc ggaatttaag gatatgaaaa
    35281 aaataaaaca cctcttaact ctgtttgaac agggagaacg tctcaccttc atgttggaaa
    35341 cagagatgga gggtgtatta attcgtattg gtacagaaaa caatttgcaa gaaatggatg
    35401 actgtagttt agttacggca agctactacc tgcataatcg cccagtgggt acaattggcg
    35461 tactgggccc gacacggatg gagtattcac gggtaattac catcattgaa gaaatcgcca
    35521 gccgactgaa tcggcttata gaggcgttat aaagaggtga ctaagatgaa tgcagaagaa
    35581 aaggtaaaga caacagcaga acaggcagaa gcagaggaga cagtacaggc agaagcagat
    35641 gcggcagcca agacacagac gacagatagt acggccgctg aggaggttac cacagccatt
    35701 gtacagctgc aggagaagaa cgagcagtta gtcagccgcc tgcatcgcct gcaggccgac
    35761 ttcgacaatt atcgtaaaag gttagccaat gagaaaaagg aatggcatac acaggctatc
    35821 tgtgaggtgg tgcgtgaatt attacctgtc attgacaatt tggaacgcgc caatgaggcc
    35881 gccggctctt tggcagcatt aaaggatggc gtagaattaa tccaccgtca actgctgacg
    35941 gccttaagta agcagggctt tgaaaagatt gaggcttgtg gtcaggaatt tgatcctaat
    36001 tttcatcacg ctgtcatgca ggtagaaggc gcggaaccgg aaaataccgt cgtagaagag
    36061 ctgcaaaaag gatatctagt caatgggcgg gttgtgcgcc caagtatggt aaaagtagca
    36121 aagtaatagt aaagggagga acataaaatg agtaaagtaa ttggcattga cctaggcacc
    36181 acaaactctt gtgttgctgt gatggaaggt ggtcaacctg aagtaattgc caatgctgaa
    36241 ggggagcgta ttactccttc tgttgtggca tttaagaaag atggtgagcg gctggtaggg
    36301 caggtcgcta aacgtcaggc gattaccaat ccagatcgca ctattatgtc tgtgaagcgg
    36361 catatgggta ctgactataa agtaaagata gatgataaaa actatacgcc ccaagaaatt
    36421 tccgctatga tcctacagaa attaaagcag gatgcagaag cctatcttgg tgagaaagtt
    36481 gagcaggctg taatcactgt acctgcttac ttcactgatg cccaacgcca ggcaactaaa
    36541 gatgccggta aaattgcggg gctggaagtc ctgcgcatta ttaatgagcc taccgcagcc
    36601 gccttggctt atggcatgga taagggtgaa gaacaaacta ttttagtttt tgatttaggc
    36661 ggcggtactt ttgatgtctc tattctcgaa ttaggagacg gcgtcttcga agtaaaagca
    36721 actagtggta acaaccgttt aggcggcgat gatttcgatg aaaaggtaat tcagtactta
    36781 gcggctcaat ttaaaaaaga tgaaggcgtt gacttgactg ctgaccgcat ggcggtgcag
    36841 cgtttgaaag aagcggcaga aaaagccaag attgaacttt ccagtgttac gaccaccaat
    36901 attaatttac ctttcattac ggctacgtca gatgggccaa agcacttgga tattacgctg
    36961 actcgtgcta agtttaatga attgacagcg gatttagtgg aagcaaccat gacaccagca
    37021 cgtaatgctc ttgcagatgc tggctttaag cctgcagaca ttgaccgggc tattctggtt
    37081 ggtggttcaa cccgtatccc cgctgtgcaa gaagcaatta aaaatttaat tggcaaagag
    37141 ccgcataaag gtgttaaccc tgatgaagta gtatccttag gtgccgctat tcaggcagga
    37201 gttttagccg gtgaagttaa ggacgtctta ctgttagatg tcacgccatt atctctgggc
    37261 attgaaactt taggtggtgt ctttacccgg attattgaaa gaaataccac gatccccact
    37321 gataaaaaac aaaccttttc caccgcggcg gataatcaaa cgcaggtaga aatccatgtc
    37381 ctgcagggag aacgttccat ggctgcaggc aataaaacgc tgggacgctt tgttttagat
    37441 ggtattccac cagcaccgcg tggtatcccc caaatcgagg tcacctttga tattgaccgt
    37501 aacggtattg tcagcgtttc ggcgaaagac ctcggtacta aaaaagaaca aaaaattacg
    37561 attaccgcgt ctacagggct tagtgataat gatatcgatg ccatggtcaa agatgccgag
    37621 aagtttgcag ctgaagacga gcaacgtaag gaactggtag aagcacgtaa tgaagctgat
    37681 aacctcgttt acagtacaga gaaaatgctg aaagatctag gcgataaagt tgatgctgcg
    37741 aaaaagacag aagtagaaac ggccattaat cagctgcgag aagaatcagc aaaagaagat
    37801 gttgccgcta ttcgtactgc tattgaaaac ctgactaaat acttgcatga gttgtcaaca
    37861 cagatgtacc aacaggagca ggctcaggca gagccgaatg ctgctggtgc cgagactgct
    37921 gatgatacgg tagacgctga atatgaggtc gtggatgacg aggatgataa aaagtaagta
    37981 aggaacggga gagcgcatgg ccaaacgaga ttattatgaa gttttaggtg tgtctacaga
    38041 agcgtctgca gaggaaatta aaaaagcgta ccgtagcttg gcacgtaagt atcatcccga
    38101 tgtaaatcgg gatgatcagg acgccgaaaa aaagtttaaa gaagccaaag aagcctttga
    38161 tgtcttaagt gatcctcaac gcagacagca gtatgatcaa tatggtcatg ccgctgaaga
    38221 aggaggattt ggcacaggcg ggtttggtca gggcgacttc tctggatttg gtggttttga
    38281 agatattttt gatcaattct tcgggggggc ggcgcgcaca cgtcgtccta ccggaccgca
    38341 gcgcggaaac gatttacgct atgatttaga aatcacttta gaagaggccg ccttgggtaa
    38401 ggaagcaaaa atcaatatcc cgcgcgctga aacttgcgat acctgcggtg gcagtggagc
    38461 caaacctggt acacaaccgg aaacttgtag tcactgccat ggtacaggtc agcagcaggt
    38521 aacacgtaat acagcttttg ggcgctttgt tagtgtccag gcgtgtgtgg catgtggtgg
    38581 gactggtacg atcattaagg agcgctgcct agattgtcaa ggtaacggac gggtacagcg
    38641 cgaacgtaaa atagacatta aaataccagc tggggtggaa actggttccc gtctgcgtgt
    38701 cgccaacgag ggcgaagctg gtacgcgtgg tgggccgcct ggtgatttgt atgttgtgct
    38761 tcatgtacga ccacataagc tttttgaccg acaaggcgat gacattatta tcgaggtacc
    38821 tatttccttt gcccaggcta ctttgggtac ggaattagaa gtgccgacca ttaccggaaa
    38881 agcccgggtt aaaatacctg aaggtacaca aactggcacc tctttccgtc tgcgcggtaa
    38941 aggaattcct catttacggg ggtacgggca gggcgatcag catgtcaagg tagttgtgaa
    39001 aataccgaaa aagttatcaa gcaagcaaaa agagctcttg cgtgaatatg ccaaacttgc
    39061 tggtgaagat gtcgctgcag gagataaggg ttttttaaat aaggtgaagg atgccttggg
    39121 cggtaaataa ggagagaata atgtgcgttg gttagaacta ataatccaca ctcgcaaaga
    39181 gagttcagaa gcaattgccg ccaaactgct ggcattgggt accggcggtg tgtcctttga
    39241 agaaagctgg ctgtgggagc aagcaaaaaa agacggttta gatgatttgt tccccacacc
    39301 aagcgcaggt ggattacaag atgatatagc tatactacgg gggtatttcc ccgtttcttt
    39361 tttaggttcg gccagcgaaa aagagttaca ctccttctta ttggcactac ctagctttgg
    39421 cttgccagag gccgacctga aatggcggta tgtcgatgat gtggagtggg aaaataggtg
    39481 gaaagattat tggttcccca ctccagtcgg taagaaactc ttaatcgtac cgacctggcg
    39541 gcaggttgaa gaagacgagg ataggttaaa aattttactc gacccgggtg ccgctttcgg
    39601 tacgggcagc cacgaaacta cccaactgtg tttgggatta ttagagatgg tgattgctgg
    39661 tggtgataag gtccttgatt taggctgcgg gtctggtatt ttgtctattg cagcccatgc
    39721 tttgggggca gaagctgttt tagggttaga ctatgatgag gtggctatcc gcatcagccg
    39781 cgaaaatgcc atccttaacg gttcaccaga gatcaaattt gttcagcgag atttatttaa
    39841 ggagacaagc tggcagacgc tgcctactgc agatgtcatt gttgccaact taacagctga
    39901 tatattactc aaaatcaagc accatttacc agctgttctg cgaccgggcg gcaaaattat
    39961 tttgtccggt attattaaag agcgtgcgga agaagtaatg accacaatct ttacccaggg
    40021 ttatcaatta ctggcagaac gcagtgctgg agaatgggtg gcactactgg gggagagaga
    40081 agcatgaatc gttttttcta tcaaacgaca cagcgagagg ctgaaattgt tcgtttacag
    40141 ggtgatgatg cacaccactt aaggcgcgtt ttaagagcag ctgtcggtga gcagatagaa
    40201 ctttgtgatg agcaggggtt atgccatcta gcacgtgtta cgcagattac taaagatgaa
    40261 gtagagtgtg ctttagcttc agccctgcca agcagtgaac cccagacaaa aattgagctt
    40321 gcttttggac tactgaaagg tgagaagaca gaacttgtgt tacaaaaggc tactgaatta
    40381 ggagtaagca gcctcagacc tttttttagc gaacgtacag tagtgcgtgc cgacaaaaag
    40441 cagggagcca ggtatgaacg ctggcagaaa attgtccgcg cggctgctgc tcaagcacgg
    40501 cgcgcccaaa ttcccgcagt agtgctgcct tgtcaatggt caagcttact aaccaagttt
    40561 gttgactatg atcaagttat tctcttttgg gaagctgaat tagaacagcc tctagcccaa
    40621 gctttacgcc attgtgcccg tggggcaaga attttactgc tgacaggacc tgaaggtggc
    40681 tttagtgaag tagaagtaag gggagcaaca gcggcaggag cagaaatagt cacgttaggc
    40741 cctcgtattt tacgtgcaga aactgcagcc atcacagctg ttgctttaac tatgtatcag
    40801 gctggagaaa tgaggagatg aagatgccaa cagcggctta ctatacttta ggatgtaaag
    40861 ttaatcaaac cgatacagct gccctgcagc agttaatgga gcaggcaggc tatactactg
    40921 tacagtttga agcaaaagct gatgtttatg tcatcaacac ctgcactgtt actcaccttg
    40981 gcgatagaaa atcccgccaa atgattcgcc gcgcccggcg cagaaattca gaagcagtga
    41041 ttgtggtagc tggctgttat gcacaagtag cagcggaaga tgtcatgagc atgccggcag
    41101 tagatttggt gattggtaca cattcacgcg agcggctgcc agaattggtc gagcaagcaa
    41161 aactagggcg tataaattct gtaaccccac tagagggcaa agtcgctttt gataatttgg
    41221 ctgttgggca gagtcctgaa catacacggg cttttttaaa agtgcaggag ggctgtcgtc
    41281 agttttgctc ttattgtatt gttccttatg cgcgagggcc actgtatagt cgacccctgg
    41341 ctgaagcagt cagcgcagca caaaaaatgg tagctcaagg tttccgtgaa gtggtcttaa
    41401 ccggtgtaca cctgggttgt tatggttttg atttcacaga ggatttggcg ctcagcgatc
    41461 tagtgcgagc tctggccgct gtcgatggct tggcacgcat tcgcatctca tctattgaac
    41521 caactgaagt aacggctgat ttaattgaag ttatgctgga taaccccaat gtctgtcacc
    41581 atttacacat tcctttgcaa agtggtgatg atgatgtttt gcagcgcatg aatcgtaaat
    41641 ataataagat ggagttttta cgtttagcgg agtggttacg aatgcaaatc tcagatatcg
    41701 ctttgaccac agatgtcatg gtgggtttcc ccggagaaac agaggaacag tttagcaaca
    41761 caatgcaagt agtacgtaaa gccgctttta gtcggctcca tgtttttaag tattctccac
    41821 gccgcggtac accagcggct aaatatccac agcaagtgcc gccgcctgtc aaaaacgaac
    41881 gcagccagcg cttaacagaa cttggccaag agctggccag caaatatcag cagggcttct
    41941 tggagcaaga ggttaatgtt ttgttcgagg aggaagttgg ggcaaaccag gtggccggct
    42001 tgacagaaca ttatgtacga gtaaaagcag caggtagtga ccagatgttg gggaaaattg
    42061 ttcctgtgag aattactgcc ctcacagctg aaggattgct tggcatctta agcgagagtt
    42121 aattcacatc ataaaaatgt actgcagcca aagaagctgg tccgtcttga ccagcttctt
    42181 tggctgcagt tttatcgttt ggtagtataa agtctaggat gtcgttattt ctttttcagc
    42241 gcttcaggca gtttaggttg atagagaatg gcccagcagg cagaagctgc accgctgaca
    42301 gcaacaagag ttagtaggct agccagaaga gagagtagtt ttttcttcat cggtctacac
    42361 ctccttctat tcgttattaa tagaaggaat actgcaagga ttcacaaaat cctgcccatt
    42421 gtcgaaacgt tacaaaacat gacagatagc ctaatcgatt tcatgctttt tgtagaacag
    42481 aggagataga gccgccaata aatgatagcc aagcggtgtt aaaagaaacg attgccacag
    42541 taatcctagg ttgccggcaa aatagtaata ggaccaaact tgtggcagct gtacagtcac
    42601 aaagcctaat actgcccata gcaagatgaa ggaaaaggaa agcagacgca gtatttttat
    42661 ttgggcgatg gaagtgattg gtttctgcgg tacagcagca ggtacatacc gataagcaag
    42721 atagcaggcg atacaaactg gtaagatgat ggcagctggt gtagcagcat agaaaggcgc
    42781 ccataagctg gtgaggtaag ctaaaataag gctggctatt gtcccgataa ttaggcagcc
    42841 gtgtgctgag gaaatatgcg cccccccact tactttgcgg taaagcatga ttactgtaat
    42901 aacagcagct gtttctttaa agatgccgaa cagtttagca agcagcaggg cgctaaagat
    42961 actaagagca gtataaacta aggaggacag gccatattca attatttcgc gttggtcgtg
    43021 tgctaatttt aattcacgcg ttaggtagtc aactaaccac ttcatggcat gctcctcatt
    43081 aataattaat accttctaga taaacgataa gcagcagctt tgtgaaagcc ccttaggcga
    43141 taagcgagca caaatactag gaaaagaaat aactgtgttg gtacggaagc agcaacgcgc
    43201 agccaggggt tggccaagat ttctgctatg gttacggaaa aagcacgggt aaaaagcggc
    43261 atagtaatgg attcaccaag cacgagaaaa acaaacccca aaatcgtggt gctaaaggat
    43321 tttttaaaat taagtttata gctatacata atgataccaa tcagggcaac ggtaatgaca
    43381 atagtatgga tgcctggagc cagcagtctg cgcatcataa aggatataat catatgtatg
    43441 cagccaacac gtacagctcc cggccagtgg ggacggaaat taatcagcgt taaaccaacc
    43501 gatgaggtga gaactccttc gggaaaagca agtaaaatta gtgtaatcag gggcatggct
    43561 tccatgaaca ttcatccttc cttgtggggt aagataaaaa aacgcagttg ttgttactac
    43621 tgctgtcctg aagtaatcct cactgctgcc tctaaagctg catttttgtg atagcctcac
    43681 tccacaggta cagggaaagt tagagtaaac tgtgtgcctt cttttaactt actttgtgct
    43741 tcaatctggc catcgtgagc agctacaatc gtttgggtga tggctaaccc taaaccagta
    43801 ccttgatctt tcgtggaaaa aaaaggatca aaaatttgcg gcattgcttc cggtttaatg
    43861 ccggtaccgg tatccttgat gataactcga attacatttg cctcttgatc aaattcacta
    43921 ctgacagtaa tgatacctcc ggcgggcatt gcctgtatgg cattggtaaa taaatttaaa
    43981 aacacctgca gcatttggct gcggtctagt aaaatgatgg gtaaataatc agctagcttt
    44041 gtttttattt ttacatcgtt ttgtatttct tgtccaacca ttaaggaaac ggttgtctct
    44101 aggatatggt taagctgcac tggtactttt cgcagttctc caggtcggct aaaccgcaaa
    44161 aagtctgtaa taatctgatt ggctcgcttt aattcatcta acatcagttg ataattttca
    44221 tcgactactg gctgtttgct ggtaagcagc tggagataac catgcactac tgtgatcggg
    44281 ttgcgaattt catgggcgat gccggcagca atttgtccgg ctaaagcaag cttttccatc
    44341 tgccttgttg tttcatgtaa ctgttcgatt tccaccaact gcttttctag cttttctata
    44401 tgttcgctaa acaaatcaac tgtaatcgct actaaaaaat agagggcgat gaaaggaaaa
    44461 atttccgcct ctttaaaagt aatcttgttt aacattaacc aattttcacc tagggagttt
    44521 attgccacga ttgtgctgat ggtagctgta acaataccag gtattaatcc gcagaaaaaa
    44581 gtagtggtga aaatcgaaat atgaaaaaga taaagaatta gtctagtttc gctgtcagaa
    44641 aaggaacccg aaagcgtcaa gtaagctaaa gctaaagttg cagtcagggc tagagcataa
    44701 acacctactt tagtgcgcat aggaaagctg cgttcagaaa aaaaacgatt tatcagacca
    44761 ctagcattgt tatccataaa ttactacccc aaaatgtgac atttcctcgg acttgtgtat
    44821 tgatttcgac aattatgaaa gttttcctct ttggcgtgtc cattatttta aaggaagaga
    44881 catattcttc ttactgtacg aataggattg atatggaaaa agacaggcgg aagagaggtg
    44941 taaagaatgg ccagaaataa aatgtttgtg gtggaagcgc ggcggatgct gccgctgctg
    45001 tttcttctag tgctcctagt ttcgttgtcg gtctatgatt cctttcgtgc cagcccaact
    45061 gcagcaccgg aaggggttgc taaggaaaat gaagtaagtt tcattaccgc ggataaagga
    45121 gaacgggagg ataatccaac cttccgcctg gcggtagatg aggaaggttg gacagaggta
    45181 gcagaggaat gggcggttac cttacctgaa taccccttcc agcctcaaca tgaggtcgcc
    45241 ttgtttgccc tacacgccga agtgcagaat gtacaaacaa gtaaagtggg ggaaaacgaa
    45301 ttagtagttg aagtacgagt taatcctaaa cgtgactatt accaggtagt tactttgcca
    45361 gcggccgatg tgatgttaga agcaggaaaa gtaacttgga agtttgtgaa taagaaaggc
    45421 agcgaaattg agcagctagt tgtagaagct actcagcaga caattgcgga agatactccg
    45481 caaaaataag caaaaggcat gccctagaga ggcatgcctt ttgcttattt gtcttttgct
    45541 tcctgtttaa ggattcttgc gcttaactgc tggcgcactg catctaaacg actgtaatcc
    45601 atagctttga tgtagtagtt ttctagttgg tcatgattgc gtttagaacc acggatcatt
    45661 tctactgctt tgcctagtaa aaaccagaga cgctccgaac tttctgatct ttcacctgag
    45721 tatttcttta gtatggtggt gttagcataa cggcttaagg caatagttgc ttgttctttg
    45781 actacttgca ccgggtttac agaaaaagta tgagggtagg taatcttgac aaaggcagtc
    45841 tttaagactg gaatgaccac agcatcaatg tggtcagggt caaagccgca gcgataaacc
    45901 tccgtatcat gcccagctcg gttgacagtt tgtcggattt gctccaagag gtatgattta
    45961 ccgcagcctg gatcgccgct tagcagatag aaacgttctg tgttttgcag aatagagggg
    46021 tagaaattga ctaacccggc aggtgtaatg gaactgccaa aaagatgacg ttctttcgct
    46081 gctcgtgtac tagcaggtaa agcggcagcc agttctgtta gcagttgatg agtcatggca
    46141 tcaactgcct ggtaatccat ggcttgcgcc attaaccagc gcaatttttc catgacgacc
    46201 gcagcttcct tcagatattg gtaggcttgc ttgtagtaat tactgttctc ctgcataaaa
    46261 tcatttattt cttttttata aggttgcaga gcagaggcat cccagaaatc acctaagtta
    46321 agaatctcat cgtaagcccc gggaataatt ggttcgacaa tatgaggcgc tgtcccatct
    46381 acgatgacat agcctaaatc agggatagag actgcatcaa gtgaggcgtg atctgaagaa
    46441 caaaaaaaat tttctgtatc gtagcctgca tccagcgctt tttgcatgac ttgtttcatg
    46501 atagtggatt tgccggtgcc gggtccgcct ttaaggataa ctaatcgttt tgtacgttca
    46561 tccgctatgt agtgaaagag agacttaaaa ccctgatagc tgttggtccc agcaaaaaaa
    46621 ttagtacttt ttcctgttgc catcaaactt cctcctttcc gtttttaatt actttattgt
    46681 atgacaggct gccttgcccg tgtctgtccg tagatgaggc taaaatatta ttt
//
LOCUS       DASRMS010000042         8554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_211038, whole genome shotgun sequence.
ACCESSION   DASRMS010000042 DASRMS010000000
VERSION     DASRMS010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 8554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8554
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_211038"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            176..667
                     /locus_tag="VFC74_02190"
     CDS             176..667
                     /locus_tag="VFC74_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24179.1"
                     /translation="MNETLPKRKKLRLEYYDYRQHGAYFVTICTRGKQHLLGEISKQR
                     NPSSAHDCVCLSRIGRVTEFEINKLSSIYDRVKIDKYVIMPNHLHLIIIINNVENGQT
                     VFAPTLSRIIKQFKGSITKQIGYPLWQRSFYDHVIRSEQDYRAKWKYIDENPWHWQED
                     EYY"
     gene            972..1763
                     /gene="moeB"
                     /locus_tag="VFC74_02195"
     CDS             972..1763
                     /gene="moeB"
                     /locus_tag="VFC74_02195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005351645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HZK24180.1"
                     /translation="MLSEEQRQRYAKHLLLPEVGEKGQEKLLAAKVLIVGAGGLGSPV
                     ALYLAAAGVGTLGIIDGDTVEPSNLQRQIVHKTASVGKLKVDSVQATLAELNPDVQVI
                     AYPEYATAENLPHILAYYDLVVDCCDNLATRYLVNDVAVLYHKPYVYGSIMRFDGQAA
                     VLYPPAGPCYRCLFPETQSSSGLSPSQDIGVLGITPGLVGMVQATEALKLILGIGESL
                     NGQLLVIDALKMEIMKVKIARDPDCVVCGDRPTILDLVSENYLSY"
     gene            complement(1820..3676)
                     /locus_tag="VFC74_02200"
     CDS             complement(1820..3676)
                     /locus_tag="VFC74_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24181.1"
                     /translation="MKIVIPPPLTEPPQPQSFGQDFWLLLRNQLHVTWNKLRHWPFLA
                     WLGVISAGIGLLSVLVYLGSVAYGALQVMPPEIGQGFLTLIFMIGIASQVFFGITAAF
                     VTLYMSEDLELFFMSPVPLRVVFAVKSLMIANSNLLTALLFCFLPGLFYGLLFQASAS
                     YYLFVILVGCGFWIMGTAAAALLNLVVMRIVPPHRSREAVGVIGAVAGILVALLFQLP
                     SMIMMQGQSLNLTDWLTGQEQLLKVMGYFPWGWGAKALGAGITGEFTNGLAWSLLLLL
                     TAAALFWVAFILLERGFRRGWISLTQGAAPRRKKQHRDLHTGEFTPSTLQFTVTEHAA
                     GVASPWAGMWAVAKKDLLYMKRDTREWFGYMVPLLLMMFFVAQYLFFQAEQTASSMVS
                     VLVMYTLMFSGNMALQSFGREGEADWFLNSVPLAGWPVVWGKLLGAVIPTLVLMQALL
                     VGTAVAIKVSASLTIMLAIGAFLLTLGASAIGLFYSINNSRFNPESPQQRISPGASML
                     MYLVNLIFMLILALGMVYLIPPAELITALKAMPPAPVGGGFLNSMGRFFFTISRPLLW
                     QPLGRTMMGLLVTFGSWSLVFFGFMAATVRQSRKGLRVENVTGTKKKRRKLK"
     gene            complement(3673..4455)
                     /locus_tag="VFC74_02205"
     CDS             complement(3673..4455)
                     /locus_tag="VFC74_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24182.1"
                     /translation="MHETLIRTQHLTKRYGSTAVVDNLNLEVQSGEIFGFLGPNGSGK
                     TTTIRMLSGLLDASEGEAYICGYNIAKEASQAKGLLAYVPDQPKIYGKLTATEFLRLV
                     AALYRVPKEFAAERAAQLLSMFGLAERADELLEGYSHGMRQKVVLAAALIHQPRVILL
                     DEPTVGLDPASARLLKNVLQELALQGAAVFLSTHILEIAERMCHRVGILQQGKLIAQG
                     SPEELRQRVGHADESLEDIFLELTGGHETAELIRSLEEGDNQ"
     gene            complement(4698..5399)
                     /locus_tag="VFC74_02210"
     CDS             complement(4698..5399)
                     /locus_tag="VFC74_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZK24183.1"
                     /translation="MERTDKTKRLALLSMFAAIILMLAFTPIGFIQLVFIKATIVHVP
                     VIIGSIILGPKAGAALGFLFGLTSFISNTITPAILSFVFSPLIPVPGTDQGSLLALVI
                     CFIPRILVGVVPWYTYRLLTKLMKGKGTMFSLAVAGVSGSLTNTLLVMNLIYFLFQDT
                     YAAARGVPVKTVYQAVLAVIFTNGIPEALVAGFITMAVCKALLSFLKTRTLQVENIPP
                     DTAAEQNSVNKKNEP"
     gene            5794..5937
                     /locus_tag="VFC74_02215"
     CDS             5794..5937
                     /locus_tag="VFC74_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24184.1"
                     /translation="MLFNVAAEMLEKIEQEFREAEPEEKERFVKSSLSLLQQIIGDSK
                     LMV"
     gene            6119..6379
                     /locus_tag="VFC74_02220"
     CDS             6119..6379
                     /locus_tag="VFC74_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24185.1"
                     /translation="MKKLIILLIITMVILLLLNHFNIIGSTPGANLPTPADPLRTQIA
                     AMSLAEKVGQLIIVGMDGTTLNNDTKKLIIDHHAGGVILYKI"
     gene            6348..7352
                     /gene="nagZ"
                     /locus_tag="VFC74_02225"
     CDS             6348..7352
                     /gene="nagZ"
                     /locus_tag="VFC74_02225"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HZK24186.1"
                     /translation="MPAVSSSIKYKKNISSSTQLLSLLNQLKETNSSNNIPLFLSVDE
                     EGGNISRMPAELEKLPANQVIGAVNNPTFSYQVGQTLAAQLKAYGFNLNFAPVLDINS
                     NPLNPVIGDRSFGHNPEIVSKLGVETIKGLQSGGVIPVGKHFPGHGDTAIDSHLGLPI
                     VNYDLTRLKNFELLPFQAAIEQRVEAIMIAHLLLPQLDDQYPASMSKVIITDLLKTQL
                     NFQGLVVTDDMTMGAIVENYPLGQAVVQAVKAGSDLILVCHDYVSQLTAFTALKNAVT
                     NGTIPEEQINASVYKILKLKQKYQISDQPLSNVDLTAINTHINDLLDTYLPTNLRATP
                     "
     gene            7451..8323
                     /locus_tag="VFC74_02230"
     CDS             7451..8323
                     /locus_tag="VFC74_02230"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012048407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HZK24187.1"
                     /translation="MITKLGRNDLCWCGSNKKYKKCHAEFDEKLETYRLQGHIVPPRK
                     IIKNPVQIAGIRASGKINTAILDSITNQIKVGISTEEIDQLVHAKTLELGGIPAPLNY
                     QGYPKSVCTSVNEQICHGIPAKDVILRDGDIVNIDVSTIYQGYFSDSSRMFCLGEVDP
                     ATAKLVQVVREAVDLGLAQVKPWHFLGDVAHAIYQHAKKHGYSVVREFGGHGIGLEFH
                     EEPWVSFVAHAGTEMLMVPGMIFTVEPMINLGSHELLFHPNGWTSYTADGKPSAQWEV
                     MVLVTEDGYEVLAH"
BASE COUNT         2500 a         1952 c         1860 g         2242 t
ORIGIN      
        1 cccatcaccc caggcgaaac gcccatcaac ctcccccacg taggggcgaa ctgtgttcgc
       61 ccgcactgat caagccccaa gcgaaacgcc catcaacatc cccccaccgt aggggcgaac
      121 ttcgttcgcc cgcacctttt agcttgcagt actttaaggt aaaggttggt gcgaaatgaa
      181 cgaaacttta cccaagagaa aaaaattgcg gcttgagtat tatgattaca gacaacatgg
      241 tgcatacttt gtgacgattt gtactcgagg caaacaacat ctactgggtg agatttcaaa
      301 gcagagaaac cctagttcag cacacgactg tgtttgtttg tcacgtattg ggagagttac
      361 cgaatttgaa attaacaaat tgtcatctat ctatgacaga gtcaaaatag ataaatatgt
      421 aattatgcct aaccacttac acctgataat aattatcaac aatgttgaga atgggcaaac
      481 agtgtttgct cctacattat cacgaattat taagcagttt aagggttcaa tcaccaagca
      541 aatcggttat cctctttggc aacgctcttt ttatgatcat gttattcgta gtgagcagga
      601 ctatcgagct aagtggaagt atattgacga aaatccatgg cattggcaag aggatgaata
      661 ttactaataa cagcagagca gtgctaaatt agcgttggtc tgcataatcc tcgggcgaac
      721 acagttcgcc cctacatttt gtttgcgcca cattttgttt gtcctacact ttttgtgcgc
      781 attattctgt ggtgggctaa atctatacgt tattgagcga aaatatgcag ctagtccgct
      841 aaacctaacg cttcttgctg acaaaggaat tagagcgaaa tttgttgtga tgaccagcat
      901 tacttggtag aattggaagt ggtacttcta acatctaaat gcgtagcaga gagaacgagg
      961 agatgatact catgttaagc gaggagcaaa ggcagcgtta cgccaaacat cttttattac
     1021 ctgaagtggg ggaaaagggg caggaaaaac tgttagctgc aaaagttctc atcgttggtg
     1081 ctggggggct aggctctccg gtggcgctct atctggctgc ggcaggggta ggtaccctag
     1141 gcattattga tggtgatact gtggagccta gtaatttgca gcggcagatt gtacataaaa
     1201 cggcaagtgt aggtaagtta aaggttgatt ctgtgcaggc gaccttagca gagctaaatc
     1261 ccgacgtaca ggttatcgcc tatcctgaat atgctacggc agaaaacctg ccgcatattt
     1321 tagcttatta tgatcttgtg gtggattgtt gtgataacct cgccacacgc tatttagtga
     1381 acgatgttgc tgtgctctac cataaaccct atgtctatgg gagtatcatg cgctttgatg
     1441 gtcaggccgc cgtactttat ccacccgctg gcccctgtta ccgctgcctg tttccggaaa
     1501 cacagtcttc cagcggcttg tcgcctagtc aagatatcgg ggtgctgggt attacaccag
     1561 ggctagtggg catggttcag gccacggaag cactaaaact tatcttgggt attggtgagt
     1621 cccttaatgg tcagttgtta gtcattgacg ccttaaaaat ggagatcatg aaagtaaaaa
     1681 tagcgcgaga tccagattgt gtagtatgtg gtgaccgccc tacaattctc gatctagtaa
     1741 gtgaaaatta cttgtcttac taaatgcaga gtaaaaaaat taggtgctgt gagggttgaa
     1801 tccctcacag cacctttatt tacttaagct ttcttctttt cttctttgtc ccagtgacat
     1861 tttctacacg caaaccctta cgactttgtc gtactgtggc ggccataaag ccaaagaaga
     1921 ctaaactcca ggaaccaaac gtcactagta gtcccatcat cgtcctgcca agtggctgcc
     1981 agagcagtgg cctgctaatc gtgaagaaga aacgccccat actgttcagg aaaccgcccc
     2041 ctaccggtgc aggaggcatt gcttttagag cagtgattaa ctccgccggc ggaatcaaat
     2101 agaccatacc taaagctaaa atgagcatga aaattaaatt aaccaggtac atcagcatag
     2161 aggcgcccgg ggaaatacgc tgttgtggac tttccggatt gaagcgagaa ttatttatgg
     2221 aatagaagag tccaatggca ctggcaccca gtgttaataa gaacgcgcca atggccagca
     2281 tgattgttaa agaagcggaa accttaattg ctactgccgt gcctaccaac agggcctgca
     2341 ttaaaactaa agtggggata acggcaccca ataatttacc ccacactaca ggccacccgg
     2401 ccagtggtac actatttaag aaccagtctg cttcaccttc tcgcccaaag gactgcagtg
     2461 ccatattgcc gctaaacatt aaggtgtaca ttactaaaac actaaccatc gaacttgctg
     2521 tttgttccgc ttggaagaaa agatactgcg cgacaaaaaa catcatcaac agtaagggaa
     2581 ccatgtagcc aaaccattcc ctagtatctc gtttcatata aagtaaatct ttttttgcta
     2641 cggcccacat tcctgcccaa ggcgaagcaa cgcctgcagc gtgttctgta acagtgaatt
     2701 gtaaggtaga gggagtaaat tcaccagtat ggaggtcacg gtgctgcttt ttgcgccgcg
     2761 gagctgctcc ttgggtgagg gaaatccagc cccggcggaa tccccgctcc agcaagataa
     2821 aggccaccca aaatagggct gctgcggtca ataatagcag caaactccag gcgagtccat
     2881 ttgtaaactc acccgtaata ccagcaccca gggctttagc tccccagccc cagggaaagt
     2941 agcccattac ttttaacagc tgttcctgtc ccgttaacca atcggtcaag ttgagtgact
     3001 gtccctgcat cataatcata ctgggcagct gaaagagaag ggcaactaaa attcccgcca
     3061 ctgcaccaat aacgcccaca gcttcccgac tgcgatgcgg cggcacaatg cgcatcacca
     3121 ctaaattcaa tagtgcagca gcggcggtgc ccataatcca gaaaccgcag cctactagga
     3181 tgacaaataa ataatagcta gcactcgctt gaaagagtag accataaaaa agaccgggta
     3241 gaaaacagaa taagagtgct gtcaggagat tactgtttgc tatcattagg gacttaacgg
     3301 caaagacaac tcgtaatggt actggagaca taaagaaaag ctctaaatcc tcggacatat
     3361 agagagttac aaaggccgcc gtaatgccga agaaaacttg cgaggcaatt cctatcataa
     3421 aaataagggt taaaaagccc tggccaattt ctggcggcat gacctgcagt gccccataag
     3481 cgacagaccc taaataaacg agcacggaga gcagccctat accggcggaa ataaccccta
     3541 accaggcaag aaagggccag tgacgcagtt tattccaggt tacatgcagt tgattgcgta
     3601 acaataacca aaaatcctgc ccaaatgatt gtggttgggg tggttcagtt aagggaggag
     3661 gaataactat tttcattggt tatccccctc ctccaggctg cgaattaatt cagcagtctc
     3721 atgaccgcct gttagctcca ggaagatatc ctctagactc tcatcagcgt gcccgaccct
     3781 ctgccgcagt tcttccggac tgccttgggc aattagtttc ccttgttgta aaatgccgac
     3841 acgatggcac attctttccg cgatctctaa aatatgggta gacaaaaaca ctgctgctcc
     3901 ctgaagtgcc aattcttgca agacattttt taataagcgg gcactggctg gatccagacc
     3961 tactgtaggc tcatctagta agataacgcg cggttggtga attagtgcgg cagccaatac
     4021 tactttttgc cgcataccat gtgaataacc ttctaataac tcatcagccc gttccgccag
     4081 accaaacata gacagtaatt gtgccgcccg ttctgcagca aactccttag gcacgcggta
     4141 taaagctgct accagccgta agaattctgt tgctgtcagc ttgccataaa ttttaggctg
     4201 atcaggcacg taggccagca atcctttagc ttgtgaagcc tctttggcaa tattgtagcc
     4261 acagatatag gcctcacctt cactggcatc taacagcccg cttaacatgc ggatagtagt
     4321 tgttttaccc gatccattcg ggcctaagaa gccaaaaatt tcgccgcttt gtacttcaag
     4381 gttaagatta tcaacgacag ctgtgctccc gtatcgtttc gtcagatgtt gtgtacgaat
     4441 taaggtttca tgcacttttc atctctcccc gctgtaaata gttttactca ttaaagaaag
     4501 attctcctag attattcaat ctcctaccaa tctttatata atttaataag acgaaaattt
     4561 gttaatattt gcattaattc aatcgctgcc ttggccaccc gacttatgcg caagcagcta
     4621 taatagaaaa ccgcctatcg tgtcccgagc aggcggctga agagctaaag cgaggcaaat
     4681 tactttaaat cagtctatta gggctcattt tttttgttta cactgttttg ctctgcggca
     4741 gtatctggtg gtatgttctc aacttgtaat gtcctggtct taaggaagga gagcaaggct
     4801 ttgcatacgg ccatagtaat aaaacctgca actaatgctt ccgggatgcc gtttgtgaag
     4861 atgacagcca gaacggcctg atacaccgtt tttaccggca cacctcgtgc tgcggcataa
     4921 gtgtcttgga agagaaagta gataagattc atcactaaca gggtgttggt cagtgatcca
     4981 gagaccccgg ccacagccag gctgaacatt gtgccttttc ccttcattag tttggtcagg
     5041 agccgatagg tataccaggg cacgacgccc actaaaatgc gggggatgaa gcagattacc
     5101 agcgccagca ggctgccctg atccgttccg ggtacaggga tcagcggaga aaagacaaag
     5161 gaaaggatgg ccggcgtgat ggtgttgctg ataaagctgg tcagcccaaa cagaaagccc
     5221 agggcagcgc cagccttcgg tcccaggata atggagccga taataaccgg tacatggaca
     5281 atagtggctt tgataaagac aagctgaata aaaccaatgg gtgtaaatgc tagcatcaaa
     5341 ataattgcag cgaacataga aagcaaagca agtctttttg ttttgtctgt tctttccata
     5401 gcaatcaacc tttccagagt ttagttactt tttcccgaca ccgcacatac tgctgctcat
     5461 ggcctttagt tactgactcc cgcatgatgc agcataagct aagcgttcgt gtaagttgat
     5521 gtcctcctta aaaagtcatc aagagatagt tgcaaaagtt tatcatgaac attaatctga
     5581 tgtcaataat gttaacatag atttgaacag gtttgaagtt tattttaaca tactaatgat
     5641 aaaataaact aaattaagtg atgccgcagg gagaggatcc tttccttata attaatttta
     5701 tgccatcctc ctgtattctt ggccaacttc ttgcaataat ttatgttccc cgagggtaac
     5761 ttaagcttaa agaaaataag cgggaggttt aagatgcttt ttaatgtcgc agcagaaatg
     5821 ctagaaaaga tagagcagga attcagagaa gcagaaccag aggaaaaaga acgttttgtt
     5881 aaaagctcac tttccttgtt acaacaaatt attggtgatt ccaaacttat ggtctaatag
     5941 caaccgcggt tgctattttt ttgtagttct ctgtcttagc gatttacata aattttcctt
     6001 tttatggcat gctactttaa tagtactgaa ttttatgtag gtagtcgcgc tagtagaccg
     6061 gaggaaacat ctttgttgac atgaacgtca aaaactcata acagcagggg gagtattaat
     6121 gaaaaagcta atcatcttgc tgattattac catggtgata ctgctactgc taaatcactt
     6181 taacatcatt ggctctacac caggtgctaa tctgcccaca cccgctgatc ccttaaggac
     6241 acaaattgcc gccatgtcgc tggccgaaaa agttggccag cttattattg tcggcatgga
     6301 cggcacaact ctgaataatg ataccaaaaa gttaatcatc gaccatcatg ccggcggtgt
     6361 catcctctat aaaatataaa aaaaatatta gcagcagtac acaactttta tccctgctta
     6421 accagctaaa agagactaac agtagcaata acattccctt atttctctcc gtcgatgaag
     6481 agggcggcaa tatcagcagg atgcctgcag agttagaaaa actgccagct aatcaagtga
     6541 ttggtgcagt taataatcct actttttctt accaagttgg acaaaccctg gcagcacaat
     6601 taaaggccta cggatttaat cttaacttcg ctcccgtctt agatattaac agcaaccccc
     6661 ttaaccccgt gatcggtgac cgttcctttg gccacaatcc agagatagtt agtaagctcg
     6721 gggttgagac aatcaaaggt ttacaaagcg ggggtgttat tccggtaggc aagcattttc
     6781 ctggtcatgg tgatacagcc atcgattctc atctcggtct gcccattgtt aattatgact
     6841 taaccagatt aaaaaacttt gagctgttac ctttccaagc agccattgag cagcgggtag
     6901 aagcgatcat gattgcccat cttcttttac cacagctaga tgatcagtat cctgcatcaa
     6961 tgtccaaagt cattattacc gacttattaa aaactcaatt aaactttcag ggtttggtgg
     7021 taactgatga tatgaccatg ggtgcaattg tggagaacta cccgctaggg caagcagtag
     7081 tccaagctgt gaaggcaggc agtgatctta tcctagtttg ccatgactat gttagccaac
     7141 tgacagcctt tacggccctg aaaaatgcag taacaaatgg tactatcccg gaagaacaaa
     7201 ttaatgccag cgtctacaaa atcctcaaat taaagcaaaa ataccagata agtgaccaac
     7261 cccttagcaa tgtcgatctc acagccatca acactcacat caatgacctt ctagatacgt
     7321 atcttcccac aaacttaagg gctactcctt aaatttagcc ttctaacagg taaaagcttc
     7381 ctgtaaagag aactggtaaa agaagaagta ttcacatcat ctctttagga gataaaataa
     7441 ggagagcgtc atgataacta aattgggaag aaacgattta tgctggtgtg gtagtaataa
     7501 aaaatataaa aagtgccatg ctgaatttga tgaaaagctg gaaacctacc gtttacaggg
     7561 acatattgtg ccgccacgta aaattattaa aaaccctgtc cagatagcag ggattcgtgc
     7621 cagcggtaaa atcaacaccg ccattttgga ttctattact aaccagataa aggtgggcat
     7681 ctccaccgag gaaatagacc aactagtcca cgcaaaaacc ctcgagttag ggggaatccc
     7741 ggcaccacta aactatcaag ggtatcctaa aagtgtctgc acttctgtta atgaacaaat
     7801 ctgccatggg atccccgcaa aagatgtaat cctacgtgac ggagatattg tgaatattga
     7861 tgtctccact atctatcaag gttacttttc tgattcttca cgtatgttct gtctcggtga
     7921 agttgacccc gcgacagcaa agctagtgca agttgtacgt gaagctgttg atttgggtct
     7981 ggcccaagta aaaccctggc actttcttgg tgacgtcgcc catgctatct accaacatgc
     8041 taaaaagcat ggctattccg tggtcagaga atttggcggc catggcattg gcctggagtt
     8101 tcatgaagaa ccctgggtta gctttgttgc ccacgccggc acagaaatgt taatggtccc
     8161 cggcatgatc ttcaccgtcg aacccatgat caatctaggt agccacgaac tcctcttcca
     8221 tcccaacggc tggacctcct ataccgcaga cggcaaacca tccgcccaat gggaagtaat
     8281 ggtattagtc acggaggatg gctatgaggt gctagcgcat taacttaaca aataagcaca
     8341 ataaaaagag ctgtaatcac catcgtggtg acaacagctc ttttatccaa gccatcagat
     8401 tgaattttag tgtgcttact tatttattcg tcaaatgcct gacttacttt acaggcatta
     8461 cccacaagat ctggagttgg ttttaatgtt tctaggcacc tccaggatga atattatcat
     8521 tttcgaatgc cattatacct ttattataca gtgt
//
LOCUS       DASRMS010000043         3845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_546758, whole genome shotgun sequence.
ACCESSION   DASRMS010000043 DASRMS010000000
VERSION     DASRMS010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3845
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546758"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="VFC74_02235"
     CDS             <1..182
                     /locus_tag="VFC74_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24188.1"
                     /translation="NIKFILFTIHIYLNFQIKLLEAYNMLNHKGTITLETDRLVLRRF
                     SLEDAEDIFNNWAND"
     gene            395..1525
                     /locus_tag="VFC74_02240"
     CDS             395..1525
                     /locus_tag="VFC74_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZK24189.1"
                     /translation="MGNLEKLLEGINALTWQNTFMMLVGCVLIYLAIKKQYEPLLLLP
                     IGFGAIMTNIPLSAAIGEQGFLTVLYNAGIVNELFPILIFISIGAMIDFSSLLKQPVT
                     LFFGAAAQFGIFAAMLLAAGANLIWPGIFSLQEAAAIGIIGAADGPTSIFVGNLFAPK
                     YMGAITVAAYSYMALVPMIQPPVIKALTTKEERKIRMDVDYDVKISKTVLILFPIVVT
                     IVAGIVAPISVPLIGSLMFGNLIRECGVLERLSQTAQNELGNLVTLLLGITIGSTMIA
                     DKFLTWDTLLILSIGLLAFVFDTAFGVLFAKSVNLFLKNKVNPMIGGCGISAFPMSAR
                     IVQKMAQEEDSSNFILMPAIGANVAGQIGSIVAGSIILALLG"
     gene            1731..3770
                     /locus_tag="VFC74_02245"
     CDS             1731..3770
                     /locus_tag="VFC74_02245"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HZK24190.1"
                     /translation="MNWAIISLSVSVLAIGVAAYFYKWVKDLPVEEGNIIQIGQLIRK
                     GAFAFLRREYRILAIFIGVVSIILFVIFPTPIWQGNAGQNILGVISYIAGSVLSGLAG
                     YVGISIATLANVRSASAAKKGLAPAYMAGFRGGAVMGMAVVSTALSGAAILQLLTGNV
                     DLVIAFSFGASTLALFAKAGGGIFTKTADIAADLTGKVELGIPEDDPRNPAVIADNVG
                     DNVGDVAGMGADLFDSQVAAIAAALVIAAPLGETNVIFCFGALGLLASIIGVLFARVG
                     KSGDPGKALNGGTYLTCGVFAILTFFASLASGFDLRIWGASFIGLLAGVIIGLTSEFF
                     TGDDKKPVAKVAEACQSGPAFTILSGFSYGLLSILPSIIGIGVAALGSYKILEPLGGN
                     YPMFGIAMSAIGMLSIVGMIISNDAYGPIVDNARGLAEMGSLGDEVLDITDKLDSAGN
                     TAKAITKGFAIGAAGLTVIALLGAFQEIIQSVTGEVISFNLMDPLVFFGILVGAAVPA
                     VFSALLMLGVNRNSMLMVAEIHRQFREIVGLKEGKPGVVPEYEKCIDIATIGAIKELI
                     PAGLSAILMTLVVGFVGGPQAMGGFLAGNIVSGLVLAMLMSNAGGLWDNAKKYIESGH
                     FGGPGSDAHKAAVIGDTVGDPFKDTAGPSINTQITVVSLIASITATLFATFSLFG"
BASE COUNT          974 a          814 c          872 g         1185 t
ORIGIN      
        1 ggaatatcaa gtttatactt ttcactatac atatatattt aaattttcaa ataaaactat
       61 tggaggctta caatatgcta aatcataaag gaacaattac acttgaaact gatcgattag
      121 tacttcgtag attttcttta gaagatgctg aagatatatt caataactgg gcaaatgatt
      181 aggtgtgaat agaatacagg caaagtatga tgtgttaaac cctggttctg gcaagggcat
      241 ggagaaacct gtccccttta tactattcac ttattgacaa aggccatata cgggaataca
      301 atatataata ggtgaacttg gctttttatg gccatttttt ctgagccaaa acttcgcttt
      361 tttagaatat tatgaagatt gaagggataa ttttatgggt aatttagaaa agttattgga
      421 gggcattaat gctttaacat ggcaaaatac attcatgatg ctcgttggat gtgttttgat
      481 ttatctggcc atcaagaagc aatatgagcc gctactcctc ctaccgatag ggtttggtgc
      541 aattatgacc aatatacccc tttctgcagc aataggtgaa caaggatttc ttaccgttct
      601 ctacaatgca ggtattgtaa atgaattgtt tcctatctta attttcattt ctatcggtgc
      661 aatgattgat ttttcctctc tcctgaaaca acctgtcacc ttgttctttg gtgctgccgc
      721 tcaattcggt atttttgcag ccatgctgct agctgccggc gccaacctca tctggcctgg
      781 tatattctcg ctgcaagaag ccgcagccat cggcatcatt ggtgcagctg acggtcccac
      841 ctctattttc gtaggcaatc tatttgcccc gaaatatatg ggggcaatta ctgtggcggc
      901 ctattcttac atggcattag ttccaatgat acaacccccg gttattaaag cattgaccac
      961 caaagaggaa cgcaagatcc gcatggatgt agattatgac gtaaagatat ccaagacggt
     1021 acttatcctg ttccccatag ttgtaaccat cgtagccggc atagtagcac ccatctcggt
     1081 accactaatc ggctcattga tgtttggtaa cttaattagg gaatgcggcg tgttggagcg
     1141 actttctcag acagctcaga atgagcttgg aaaccttgtt acactgctat tgggtatcac
     1201 cattggatcc accatgatag cagataagtt cctaacatgg gatactctgc tgattctttc
     1261 catcggcctt ctagcatttg tattcgatac cgctttcgga gtactatttg caaagtctgt
     1321 aaaccttttc ctgaagaata aggtcaatcc catgattggc ggttgtggta tatcggcatt
     1381 ccccatgtct gcccgtatcg ttcaaaagat ggctcaggag gaagattcat ccaactttat
     1441 cttgatgcct gccatcggag caaacgttgc cggacaaata ggatccatcg ttgccgggtc
     1501 aattatcctg gcactacttg gttaggaggg tatataaatg gatatatttt taggcgcact
     1561 tgaagtaatg ggcatcggcg taaccggtgt ttttgttgtg ttagccctat tttatggact
     1621 aaccaaacta atgatcacac tttttcccgc tgattgacat aacttattta tataggataa
     1681 tttttgaaaa tctaaaataa taaaataata agtttagggg gcaaggtaaa atgaattggg
     1741 ctattatttc actctcagta tctgttttag caattggcgt agcggcgtat ttctacaaat
     1801 gggttaaaga cctcccggtg gaagaaggaa acataatcca aattggccag ttaatccgca
     1861 aaggggcatt tgcgttctta aggcgagaat atcgcattct agctattttt attggtgtag
     1921 tatccatcat tctttttgtc atatttccca ctcccatatg gcagggcaat gcaggacaaa
     1981 atattctagg cgtcatttcc tatattgctg gttcagtgct gtccggttta gcaggttatg
     2041 ttggaattag cattgctacc ctggccaatg ttcgttctgc ttccgctgct aaaaaaggcc
     2101 ttgcaccagc ttatatggca ggcttccgcg gtggtgcagt aatggggatg gcagttgtca
     2161 gtactgcact ttccggtgcg gccattcttc aactgctaac aggcaatgta gacttggtta
     2221 ttgcattcag ctttggtgcc agcaccttag cattgtttgc aaaagccggt ggcggaatct
     2281 tcaccaaaac cgccgatata gcagcagact tgactggtaa ggttgaactg ggcatcccag
     2341 aggatgatcc ccgtaatccc gctgttattg ctgacaacgt tggcgataac gtaggtgacg
     2401 tagctggtat gggtgcggat ctgttcgact cccaagtagc tgccattgca gctgctctag
     2461 ttattgcagc accccttggt gaaactaatg ttatattctg ctttggtgct ttaggcctat
     2521 tggcttctat tattggtgta ttattcgccc gtgttgggaa aagtggagat cctggtaaag
     2581 cactgaatgg cggcacctat ctcacttgtg gtgtatttgc catactgaca ttcttcgcaa
     2641 gtctggcttc tggatttgac ctacgcatct ggggcgcatc cttcatcgga ctgctggctg
     2701 gtgttattat cggtctaacc agtgaattct ttaccggcga tgacaaaaaa cccgttgcca
     2761 aggttgccga ggcctgccaa agtggccctg cctttaccat cctttcgggt ttttcttatg
     2821 gtcttttaag cattttgcct tccataattg gtatcggcgt tgctgcatta ggctcatata
     2881 aaatcttaga acctttggga ggaaattatc ccatgttcgg aattgcaatg tctgcaatcg
     2941 gtatgctgtc catcgtagga atgattattt ccaatgatgc ctatggcccc attgttgaca
     3001 atgcccgtgg ccttgcagaa atgggtagct taggggatga agtgcttgat ataacagaca
     3061 agcttgactc tgctggtaat acggctaaag ccatcacaaa gggctttgca attggtgcag
     3121 ccgggcttac agttattgca ctcttgggtg cattccaaga gatcatacaa tccgtaacag
     3181 gtgaagtcat ttcctttaat ctgatggatc ccctagtctt cttcggaata ctggtcggtg
     3241 cagccgtccc tgctgtattt tcggccttac ttatgttggg ggtaaaccgc aactccatgt
     3301 tgatggtggc tgaaatccac agacaattcc gagaaattgt tggcctaaag gaaggcaagc
     3361 caggagtcgt gcccgaatat gaaaaatgta ttgatattgc aaccattggt gcgatcaaag
     3421 aattaatccc tgccggactt agcgcaatcc ttatgacctt ggtggtaggc tttgtgggcg
     3481 gtccacaggc aatgggcggc ttccttgcag gtaatattgt ttccggtttg gtcctagcta
     3541 tgctgatgag caatgctggc ggactgtggg ataatgcaaa gaaatacata gaatctggtc
     3601 actttggggg cccaggtagc gatgctcata aagcagctgt tatcggcgac accgtaggcg
     3661 atcccttcaa ggataccgcc ggtccttcca tcaataccca gattactgtt gtatccctga
     3721 ttgcttctat tacagcaaca ttatttgcaa ccttctcttt gtttggctga tcaaaaacta
     3781 aacaccctgc tggcgaatct cggcagggtg tttttatttt tggcattttc cgcttaatat
     3841 ccaat
//
LOCUS       DASRMS010000044         1091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_748360, whole genome shotgun sequence.
ACCESSION   DASRMS010000044 DASRMS010000000
VERSION     DASRMS010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1091
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748360"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(53..>1091)
                     /locus_tag="VFC74_02250"
     CDS             complement(53..>1091)
                     /locus_tag="VFC74_02250"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HZK24191.1"
                     /translation="PWNYPFQLTLNPIIGALAGGNAVIVKPSAYSPHTSEIIAKICAD
                     AFPNGLVQVIQGGRKENQALLDMPFDYIFFTGSPEVGRVVMAAAASNLTPLTLELGGK
                     SPVIVEHTADIRKTAKRILFGKLLNAGQTCVAPDHILCERRVKAELLSELKQQSQAIF
                     ADLEYVKVAWPKIITENHYKRILNLIDQENIVVGGKGYPDTRQIEFTILDQPSWDSSV
                     MTEEIFGPVLPVLAWDDLDQLIHEQKMRPKPLALYIFSKNKTVNDKVLNELSFGGGCV
                     NDTVMHLSSSKAPFGGVGNSGMGNYHGEYSFRTFTHAKTILQKWWSFDLPVRYHPYKD
                     PKSRLPEILLR"
BASE COUNT          302 a          235 c          234 g          320 t
ORIGIN      
        1 agcttgctgc tcctgtgtaa gaatcataat ttcatctcct ctactctaat cattaacgca
       61 aaagaatttc tggcaggcgt gattttggat ccttgtaggg atggtagcgc acaggcaggt
      121 caaaactcca ccatttctgt aggatagttt tcgcgtgggt gaaagtgcgg aagctgtact
      181 cgccatgata gttacccatt ccgctattgc ctactccgcc aaacggtgct ttcgatgatg
      241 aaagatgcat aacagtgtca tttacgcagc caccgccaaa agagagttcg ttcagaactt
      301 tatcgttaac agttttattt tttgaaaaaa tgtaaagtgc tagcggtttg ggtctcatct
      361 tttgctcatg aattaactgg tccagatcat cccaggctaa aacaggtaac acgggaccaa
      421 aaatttcttc agtcatcact gatgaatccc aggatggctg gtctaaaatt gtaaattcta
      481 tttgcctggt atcaggatat cctttaccac cgactacgat attttcctga tcaattaggt
      541 tcaaaattcg tttataatgg ttttcggtta ttatcttggg ccaggcaact ttcacgtatt
      601 ccagatcggc aaaaatagcc tgagactgtt gttttaattc gcttaaaagt tctgccttta
      661 ctcgacgctc gcataaaata tgatcaggtg caacacaagt ttgacccgca tttaacaatt
      721 taccaaataa tatgcgctta gctgttttcc tgatatcggc tgtatgttca acgatgaccg
      781 gtgatttacc accaagttcg agggtgagcg gtgtcaaatt actagcagct gctgccatga
      841 cgacacgtcc gacttcggga ctgccagtaa agaaaatata atcaaagggc atatcaagga
      901 gtgcttggtt ttctttgcgt ccaccctgga tgacctgaac cagtccgtta ggaaaggcat
      961 cagcacatat ttttgcgata atctcagatg tatgcggact gtaggctgat ggctttacaa
     1021 tcacagcatt gccaccagcc agagcaccaa taattggatt aagtgtcagc tggaaaggat
     1081 agttccaagg a
//
LOCUS       DASRMS010000045         1058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_10375, whole genome shotgun sequence.
ACCESSION   DASRMS010000045 DASRMS010000000
VERSION     DASRMS010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1058
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10375"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="VFC74_02255"
     CDS             complement(<1..386)
                     /locus_tag="VFC74_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012993061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK24192.1"
                     /translation="MTIFQKAIKKDNTFLADSVDIIVVGAGHAGCEAALAAARLGKSC
                     ILFTMTLDSLANLPCNPNIGGTAKGQLVREIDALGGEMGRIADRNMIQFRMLNASKGP
                     AVISPRAQMDRVHYQRDMKSILEQEK"
     gene            complement(439..>1058)
                     /locus_tag="VFC74_02260"
     CDS             complement(439..>1058)
                     /locus_tag="VFC74_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24193.1"
                     /translation="DPIEQEGIKRAQEALQNADLVLWVLSPPLGSSGELRDEMNTIKT
                     ILDRQQQVLLILGKDDLRSSVPAEEDPASFAGKYLPDVPTIAWSAKREEDLNALRNIL
                     LNFIEHDTLELDVQSENFAVRPEDHGVQKQTSAAIVTHSRHKSLADKSLEQVKSAREA
                     IDAGLSYDLIATLLRSAVESLAAMSGDDVSETLIDTIFSRFCIGK"
BASE COUNT          283 a          244 c          218 g          313 t
ORIGIN      
        1 tttttttctt gttccagtat gcttttcatg tctctttggt aatggacacg atccatttga
       61 gcacgcggag aaataacagc cggacctttg gacgcgttta gcatacgaaa ctgaatcata
      121 ttgcggtcag ctattctgcc catttcaccg ccaagtgcat ctatttcacg caccagctgc
      181 cctttagcag tgccgccaat attagggtta cagggtaaat tggccaggct atccaaagtc
      241 atggtaaaga gaatgcaaga cttacctaat cgtgctgcag ctaaggcagc ttcacaaccg
      301 gcatgtccgg cgcctaccac tatgatatcg acgctgtctg cgagaaaggt attatctttt
      361 tttattgctt tttgaaaaat agtcatattt atcttttata aattaaaatc agcaaaaact
      421 gattattgtt agtaattgtt atttgccaat gcaaaaacgc gagaaaatag tatcaatcag
      481 ggtctcggaa acatcgtcac cactcattgc tgccaagctt tcaactgcag accgcaacaa
      541 agtagctatc agatcgtagg agagaccggc gtcaatggct tcacgtgcag atttaacttg
      601 ctccaaactt ttatctgcca aagatttatg tctggaatga gttactatcg ctgctgaagt
      661 ctgtttttga accccgtgat cttcaggcct gactgcaaaa ttttctgact gcacgtctaa
      721 ttccaaagta tcatgttcaa taaagtttaa aaggatattg cgtaaggcgt ttaaatcttc
      781 ctcgcgcttt gcagaccagg ctatggttgg cacatcaggc agatattttc cggcaaagct
      841 ggctggatct tcttcagcgg gtacagagct gcgtaaatca tctttcccca gaataagtaa
      901 gacttgctgt tgccggtcca atatagtctt aattgtgttc atttcatccc gaagctcacc
      961 agaacttcca agtggcggcg acaacaccca gagcacaagg tcggcgtttt gaagtgcttc
     1021 ctgtgctcgc ttaattccct cttgctcaat cggatcat
//
LOCUS       DASRMS010000046         2836 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_144830, whole genome shotgun sequence.
ACCESSION   DASRMS010000046 DASRMS010000000
VERSION     DASRMS010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2836)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2836)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2836
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144830"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..295
                     /locus_tag="VFC74_02265"
     CDS             <1..295
                     /locus_tag="VFC74_02265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK24194.1"
                     /translation="WLRRITTWEEQKMQMLAAPVSSQPELMTIKKETNSQVIELLQQL
                     PVKYREVLVLYYLQELSINEVSHILGQPEGTVKSKLSRGRTKLKELWDERESE"
     gene            276..647
                     /locus_tag="VFC74_02270"
     CDS             276..647
                     /locus_tag="VFC74_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24195.1"
                     /translation="MKGRVNKVDAFDQTVSEKLQQELQVVQLSSSQKEELGASMIGEL
                     QANHLSAGQRLLRKIKIFWQSTYEVSLAPTAATICILLVVAGVKWLHPTTTKPVEPTY
                     FIRQTDAGLEITPLENTREVE"
     gene            649..>2836
                     /locus_tag="VFC74_02275"
     CDS             649..>2836
                     /locus_tag="VFC74_02275"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK24196.1"
                     /translation="MFRARLQGKHLVFITVLMFVLTGLIITAVIRDGRVQAAHDLEQA
                     AKNGAWDEYLRLLQEQEPNPAEKLYSSATEDAEGAPDWQRDTIYLFPTWTHGGDATGQ
                     EVHLDWAIAKLLLLQEHYPDSSWKSHALRDLATYYYALGDYTQAEKYAKAVDDVLLLA
                     RIALDRGDYQRALQITEQELQGEANPKMELLYAKGRALLGLGEWEEAKKLFTSLPAAA
                     EAMLAPFLEDEQMIRDNVGHWEQIAQLNLERITTLQNSEGTGQIGGRVLLGGVPLAGV
                     RVYLLDNTVPKNWSSSNEVMTMRQVVSNAEGTFQFKHVLPGKYVLGAAVQLAAVEGYT
                     LQEQQEFTVESGQNVTQELRFVEVALLEEPIGRQEVDGEVEFRWQAVEDAAFYKLWVT
                     SVVDQTGSVSTVLRPQVTANSIRINLTEELKKSPFYPSYGYGSEKLNPHLLFGQIYRG
                     GEFAWYITAHDASGRKISASNGYGATVSEEELPLFKIKGEFSPADRLVWAQEYERAIA
                     AYEASLKNNPQDSHALVMLARIHHFGIRRGEAKPAEAAGYYERLLNVDDTPEARKALA
                     EVYAQLKRNQEAYELYQSLLGTPAADWQLHYELAKVEYQLGRPSAALTRLKQTVTMAD
                     GEYLRVYPVALSLLLGDVDSALWFAQQVDEGERYLSLLKAYEAAEYTSSPAVEQAIKT
                     GEFVQAGELLTQKPHDLFLQTLLLYLEGNSAVELREQMLPSFSPGLL"
BASE COUNT          790 a          586 c          763 g          697 t
ORIGIN      
        1 ttggctgcgt cgcatcacaa cctgggaaga gcagaaaatg caaatgctag cggcaccggt
       61 gtcttcacaa ccagagttga tgacgatcaa gaaggaaaca aatagtcaag tgatagagct
      121 gctgcagcaa ctgcctgtta aatatcgtga ggtattggtg ctttattatc tacaggagtt
      181 aagtatcaat gaagtaagtc acattttagg gcagcctgaa ggcacagtta aaagtaaatt
      241 atcccgcggc cgcactaagc ttaaggagct atgggatgaa agggagagtg aataaagtgg
      301 atgcgtttga tcaaactgtg agtgaaaagt tgcagcaaga gctgcaggtt gtgcaactaa
      361 gcagttcgca aaaagaagaa ctgggcgcat ccatgattgg ggagctgcag gccaatcact
      421 taagtgcagg ccagcgtctg ctgcgcaaga taaagatttt ttggcaatct acctatgaag
      481 tttccctggc gccaacggca gcgactatct gtattctttt agtggtagca ggtgtaaagt
      541 ggttacaccc cacaactact aaaccggttg aacctactta ttttattcgg caaacagatg
      601 cgggtctcga gattaccccg ctagaaaata cgcgggaggt agaatagcat gttcagggct
      661 cgtttgcagg gaaagcatct agtattcatt acagtgttga tgtttgtgtt aacaggtctc
      721 attattacgg cagtaattcg tgatggtcgt gtgcaggctg cacacgattt agagcaagca
      781 gccaagaacg gggcttggga tgagtatttg cggctgctgc aggaacaaga accaaacccg
      841 gcagaaaaac tctatagctc cgctacagag gatgccgagg gtgcacctga ttggcaaaga
      901 gatactatct atctttttcc tacttggacc cacgggggtg atgccacagg ccaggaagtg
      961 cacctggact gggcgatagc taaacttctc ctcctgcaag agcattaccc tgatagctcc
     1021 tggaagagtc acgctttgcg ggatttagca acttattatt atgccctggg cgactatacg
     1081 caggcggaaa agtatgctaa agcggttgat gatgttttac tgttagcgcg catagcttta
     1141 gaccggggtg actatcaacg ggcattacag atcacagagc aagagctgca gggtgaagcc
     1201 aaccccaaaa tggaactact gtatgccaag ggaagggcac tgttaggcct gggagagtgg
     1261 gaagaagcaa aaaaactatt taccagccta ccagctgcag cggaagccat gttagctcca
     1321 tttttagaag atgagcaaat gattcgtgat aatgtcggtc actgggagca aatagcccaa
     1381 cttaacctgg agcgtattac tacactgcaa aatagtgagg gcacggggca gattggtggc
     1441 cgtgtactat tgggtggagt gccgctagct ggcgtgcgag tatatttgct tgataacact
     1501 gtgcccaaaa actggtccag ctccaatgaa gtaatgacga tgcgacaggt tgtcagtaat
     1561 gctgagggca ccttccagtt taaacatgtg ttgcccggaa agtatgtttt aggggcagcg
     1621 gtacagttgg cggcggtaga aggatataca ctgcaggaac aacaggagtt taccgtagaa
     1681 agcggccaga atgtgacaca agagctgcgc tttgtcgagg tagccttact agaggagcct
     1741 atcggcagac aggaagtgga cggagaggtg gaatttcgct ggcaggcagt ggaagatgca
     1801 gccttttata agttgtgggt cacatctgtc gtcgatcaga cgggttcagt tagtacagtc
     1861 ttgcggcctc aggtcacagc taactccata cgaattaacc tcactgagga actaaagaag
     1921 tcaccttttt atccaagtta cggatacggc agtgaaaaat taaatcccca cttactcttc
     1981 ggacagattt atcgtggtgg tgaatttgcc tggtatatca ctgctcacga tgcctcaggt
     2041 cgtaaaatta gtgccagcaa tggttatggt gccactgtca gtgaagagga gttacctcta
     2101 tttaaaataa agggtgagtt ttccccagct gaccgtttag tatgggcaca ggaatatgag
     2161 cgggcgattg ctgcctatga agctagttta aaaaacaatc cccaggatag ccatgcttta
     2221 gttatgctgg ccagaatcca tcattttggc atccgccgtg gtgaagcaaa acctgcagaa
     2281 gcggcgggtt actacgaacg cttactgaat gtggacgata caccagaagc acgaaaagct
     2341 ttggcagaag tttatgccca attaaaacgt aatcaggagg cgtacgaact ttatcaatca
     2401 ctgttaggta cacctgcggc agactggcag ctgcattatg aattagcgaa agttgagtac
     2461 cagttgggtc ggccctctgc cgctctgaca agacttaagc aaactgtaac catggctgac
     2521 ggtgaatatc tgcgtgttta tccggtagca ttatctttac tcttaggtga tgtggacagc
     2581 gccttgtggt ttgcccaaca ggtggatgaa ggtgaacgtt atctgtcgct gcttaaagca
     2641 tatgaggcag ccgagtatac ttcttcccca gctgtagagc aggccattaa aactggtgaa
     2701 tttgtgcagg caggtgaatt attgacgcag aaacctcatg acttgttttt gcaaacgcta
     2761 ttattatatt tagagggtaa tagtgcagtc gagttgcggg agcaaatgct gccaagcttt
     2821 tcaccgggac tattac
//
LOCUS       DASRMS010000047         2476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_77797, whole genome shotgun sequence.
ACCESSION   DASRMS010000047 DASRMS010000000
VERSION     DASRMS010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2476
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77797"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..820)
                     /locus_tag="VFC74_02280"
     CDS             complement(<1..820)
                     /locus_tag="VFC74_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24197.1"
                     /translation="MSRHAINLLMDSGLLTAAGRPADLSGLPENEVSARISQYINGRV
                     QTIDAEIVSFDPPRRLNALFGSESTEASAPKILPSALVYESIIIDDPLVSASSSISYD
                     SIEEGLRLFSSYFQLIKADIIKVIPLSLLNRPSDEVPLLVSDDAFRSSIPDELHDFIH
                     SRANLRSVVRDDQGRMLVLKEDASKKRRPALNVMFRGDFWKKGVQLYLFQTVEKVSEI
                     DGGIKCRQIWQPDKFLDEETFERWSYQTINQAMRVRLRNIYNETRLAGELGHTYI"
     gene            1040..1927
                     /locus_tag="VFC74_02285"
     CDS             1040..1927
                     /locus_tag="VFC74_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integron integrase"
                     /protein_id="HZK24198.1"
                     /translation="MPQTPKPAPKLRDQLHAIIRTKHYSLRTEKTYWYWIRYFIRFHR
                     LRHPRDMAEAEVAQFLTWLATHKNVAAATQNQALNALVFLYKHVLQQPLQHIDGIARA
                     KKPRKLPVVLSHEEAISVIAQLPPPHRLIASLMYGAGLRVSEACRLRIKDVDFNLHRL
                     VIRSSKGGKDRTTLLPQTLFTELEELIAQRTHQHASQSGMPAPVTLPYALARKYPNAG
                     CALAWQWLFPSESLCQDDDGRWVRHHLHISAVQKAVKKSIAAAGIHKAASCHSFRHTF
                     ATQLLCSALGHPEVFLADS"
     gene            complement(1886..>2476)
                     /locus_tag="VFC74_02290"
     CDS             complement(1886..>2476)
                     /locus_tag="VFC74_02290"
                     /inference="COORDINATES: protein motif:HMM:NF033559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZK24199.1"
                     /translation="VHYSSKRAYRDAENRRKGLERLKQRVQSGQLTKQSLNNRGYNKY
                     LKLHGDVTIEIDMQKYEQDAVWDGLKGYTTNTKLSEQELLEHYGQLWHIEKAFRMSKT
                     DLRVRPIYHRLENRIRAHMSIVFTAYCIYKTLETALKQEHSSLSLEKAAEVTQNMYQV
                     VLDLPGQQPQKIRLGMDEQQQELLTICQKHFRVSQR"
BASE COUNT          628 a          635 c          530 g          683 t
ORIGIN      
        1 ttatatatgt gtggcccagt tcaccagcta gcctagtttc attgtagatg tttcttagcc
       61 taactctcat tgcctgatta attgtttgat aggaccaccg ttcaaacgtt tcttcatcaa
      121 gaaatttatc aggctgccat atctgcctac acttaatgcc gccatcaatt tcgctaactt
      181 tctcaactgt ctggaaaaga tatagctgaa cacccttttt ccaaaaatca cctcgaaaca
      241 ttacatttaa agctggtcta cgtttctttg aggcatcttc cttcaagacc aacatcctgc
      301 cctgatcatc tcgcactaca gatcttaggt tcgcacgact gtgtatgaag tcatgcaact
      361 cgtcgggtat gctggatcta aacgcatcgt cagaaaccaa caatggaact tcatcgcttg
      421 gcctattaag tagcgatagc ggtattacct ttatgatatc tgctttaatc aattgaaaat
      481 atgaagaaaa taacctcaaa ccttcttcaa tggaatcata gctaatacta gagctagccg
      541 aaaccagagg gtcatcaatt ataatcgatt cataaacaag agctgatggc agtatttttg
      601 gcgccgatgc ttccgtggac tcggatccaa aaagtgcatt aagtctgcgg ggaggatcaa
      661 aggatactat ctcggcatca atagtctgaa ccctaccgtt tatatactga gagattcgcg
      721 cggagacctc attttcgggt aggccagaca aatcggcggg ccttcctgcc gctgtgagca
      781 gtcccgaatc catcaagagg tttatcgcat gcctactcac attgtctcca gaaaaacata
      841 acatgtatta gatcgccttc cacataagcc aatcatcgtg aaggcggtat aacctgatgc
      901 gactaaattt tgatttcccg attatactat agggatcaac ccgttacaag accatacttt
      961 ttggtagatt attttcgctt gacccgcctt gtcttcacca gcaagatact gtttatccat
     1021 acagcaaaag gaaattgcta tgccccagac acccaagcct gcacccaagc tacgcgatca
     1081 gctgcatgca atcattcgta ccaaacatta cagcctgcgt actgaaaaaa cctactggta
     1141 ctggatacgc tatttcatac gctttcatcg actccgccac ccccgcgaca tggccgaagc
     1201 agaagtcgct cagtttctga cctggctggc gacacacaag aacgtggcgg cggccaccca
     1261 aaaccaggcg ctcaatgcgc tggtatttct ttataaacac gttttacaac agcctttgca
     1321 gcatatcgac ggcattgccc gtgctaaaaa gccacgcaag ctgcccgtgg tgctgtccca
     1381 tgaggaagcc atatccgtca ttgcccagct gcctcccccg catcggctga ttgcttcgct
     1441 catgtatggg gccggtctgc gtgtcagcga agcctgccgt ttacgcatca aggatgtgga
     1501 ttttaatctg cacaggctgg tgatacgcag cagcaagggt ggtaaagatc gcacgacctt
     1561 gctgccccag acgctgttta cagagcttga agagctgata gcgcaacgga ctcaccaaca
     1621 tgccagccag tccggcatgc cagcacctgt tacgttgcct tatgcgctgg cccgtaaata
     1681 tcccaatgca ggctgcgcac tggcttggca atggctgttt ccttctgaga gtctatgcca
     1741 agacgacgac ggccgttggg tacgccatca tttgcatatt tctgcagtac aaaaagcagt
     1801 gaaaaaatct attgcggcag ctggcataca caaggctgcc tcctgccaca gttttcgtca
     1861 cacgtttgca acccagctcc tgtgttcagc gttgggacac cctgaagtgt ttttggcaga
     1921 tagttagtaa ttcttgctgc tgttcatcca taccaagtcg tattttctga ggctgttgtc
     1981 caggcaaatc taagacgact tggtacatgt tttgtgttac ctcagccgcc ttttcaagcg
     2041 agagtgatga gtgctcttgc ttaagcgctg tttccaaggt tttatagata cagtacgccg
     2101 taaaaacgat gctcatatgt gcacgtattc tgttttctaa acgatggtaa attggccgga
     2161 ctcttaagtc cgtctttgac atccggaagg ccttttcgat atgccagagt tggccataat
     2221 gctcaagcaa ctcttgttca gagagttttg tgttggtggt atagcctttc aggccatccc
     2281 acaccgcatc ctgctcatac ttctgcatat caatctcaat ggtgacatca ccgtgtaatt
     2341 tgaggtactt gttataacct cggttattca aactttgttt cgtcagttgt cctgactgga
     2401 cacgctgttt aagacgctct aggcctttac gacgattctc tgcgtcacgg taagcacgtt
     2461 tactgctgta gtgaac
//
LOCUS       DASRMS010000048        10306 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_819679, whole genome shotgun sequence.
ACCESSION   DASRMS010000048 DASRMS010000000
VERSION     DASRMS010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 10306)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10306)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10306
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_819679"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1098)
                     /locus_tag="VFC74_02295"
     CDS             complement(<1..1098)
                     /locus_tag="VFC74_02295"
                     /inference="COORDINATES: protein motif:HMM:NF033592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HZK24200.1"
                     /translation="MTEKKTLFKGLLITERLINDIIFMCESRTKPTYFTRAANCKMDF
                     KSLILFQLNFVKKTLQLELDSFFKTIKGGTMSITKQGYSEARQKISPTAFIKMADAIT
                     SWYYGDDNFKKFKGYRLCAIDASILEINNSQRLRDAYGYSEGSKTVKLARAKASSIYD
                     IENDMIITSKISKYTTGEREMAVELIEKLKELGLKNDLILFDRGYPSKEFFEYLDKSG
                     IKYVCRISSQKIRQIHQAQKPDQVIELLVKVRHPKLNVRVLRFGLDSGEEEVLVTNLF
                     DESLNTHDFKKLYFKRWGIEVKFNELKSKLQIENFTGDTAISVEQDFYASIYLSNMAA
                     LARNEANEKVAQNNKGKNLKYDYKVNINMLIG"
     gene            complement(1318..2430)
                     /locus_tag="VFC74_02300"
     CDS             complement(1318..2430)
                     /locus_tag="VFC74_02300"
                     /inference="COORDINATES: protein motif:HMM:NF025644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HZK24201.1"
                     /translation="MQSYHKALTITMVLYLGIFCLLFAVLPKRTFSEAENRYLQSRPA
                     FSWDKLLSGEFGQTAEKYLSDQFILRDTWISLKAKAELLLQKNDHHGVYFGREGYLLQ
                     KPSQQEKEQVGRNIAAVNEFAKQVNGRIYFMLAPVSAQILRDKLPRYAAPQIIEPGEV
                     RSQMSKEITFIDPTAVLESQRQENIYYKTDHHWTTRGAYFAYQEAAKIMGVTALPQDA
                     YTVKQVSDNFYGTLYSQSGYPCQPDTIQLFVPQKKQQYTVAYADKHKISDSVYACEHL
                     QKKDQYALFLDGNHALIKITTPNQAGKKLLIIKDSYANALVPFLIPHFSEIHVLDLRY
                     FHHQVKDYFEEKGLEEIFFVYNTITFAAETSLRKLR"
     gene            complement(2445..3851)
                     /locus_tag="VFC74_02305"
     CDS             complement(2445..3851)
                     /locus_tag="VFC74_02305"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZK24202.1"
                     /translation="MIFSSLFFLFLFLPLVLLSYYLLPQSCRNAALFLASLIFYAWGE
                     PIYIWLMLFSIVINYLYGLLLARYEKQPKLAKIILYSSLALNLGLLFFFKYADLISQS
                     INFVFGTALQELNLPLPIGISFYTFQTLSYLLDLYRAEVLPQRNLIAFGTYVSLFPQL
                     IAGPIVRYQTVAEQLLQRREDISSFGRGVSRLIIGLAKKVLLANNLGLLWQEVQQLPA
                     AELTISGAWLGIIAFALQLYFDFSGYSDMAIGLGQMFGFTFLENFNYPYLAQSITDFW
                     RRWHISLGTWFREYVYIPLGGNRRGPRLMYRNLLVVWCLTGLWHGASWNFMLWGLYFG
                     LILAMEKAFLGRWLQLLPCLLRHLYAKVLILLGWVLFVFTDLVKAGQYWQAMFGFNSN
                     GWLDQQTLYLLTNYGLLLALAVLCSVPWSKKTIRWGENISPAWTYAAAVAAQILLLML
                     TTAYLVDSTYNPFLYFRF"
     gene            complement(3857..4336)
                     /locus_tag="VFC74_02310"
     CDS             complement(3857..4336)
                     /locus_tag="VFC74_02310"
                     /inference="COORDINATES: protein motif:HMM:NF025628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4358 domain-containing protein"
                     /protein_id="HZK24203.1"
                     /translation="MRRKICLSWVMLLMLLAAGCGQSSKLNLQAAECAEVLWERIPFT
                     DTLSAIDEQMSMVLYPVDEKDVAAQQVYVSTGATAEEIAVFEAVDAAAAERLKEVVLQ
                     RLAEQKASYADYLPVELPKLEDPYLRVTGPYVIFCVSGHNEKVSKEVEQLLYGNEEG"
     gene            complement(4326..5150)
                     /locus_tag="VFC74_02315"
     CDS             complement(4326..5150)
                     /locus_tag="VFC74_02315"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HZK24204.1"
                     /translation="MAAKRGRWQVLNFTVLVLISLIILFYFSPGFKQELAVAQTEEIE
                     PPLMPAGEPNQQSSAYDFHSAVPAAAGTIAEDYFQDAVFIGDSRTTGLQLFGGPTEAI
                     YYTATGLKVDTISTKPVVASGKQKITIMEALDQHKFDKIYIMLGINELGWAYSDLFIK
                     TYGEVIAELKKIAPAALIYVQSILPVTKKKSLQDNVYNQQNINKYNELIQQMAAEQQV
                     YFLDAAAAVKDDEGYLPSAATTDGIHLNPAYCQLWFDYLQQYYLVGTTGGVVKDAS"
     gene            5341..6048
                     /locus_tag="VFC74_02320"
     CDS             5341..6048
                     /locus_tag="VFC74_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK24205.1"
                     /translation="MTASKVLVVEDELSIRKFIAINLHRNNFEVLEAADGETALTLVQ
                     AYSPEVVILDIMLPTIDGFEVCLQIRQLRPQTVIIMLTAKGEDLDKVMGLDLGADDYM
                     VKPFNPLELIARIRAHLRKISVLPQQSNFLHFRQLKLDLEGQRFYKDKQSIELTPTEF
                     AIMKLFMQQPQKAFTRNEILNSIWGQNYFGDLKTVDVHIRRIREKLEDNPSDPQWLET
                     VWGLGYRLKGDSSNAQY"
     gene            6035..7423
                     /locus_tag="VFC74_02325"
     CDS             6035..7423
                     /locus_tag="VFC74_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK24206.1"
                     /translation="MRSIKHRMVLYFMFIIIITVIIFEFFLITSLRKNYYQSLEDSLL
                     NQLQTSAELYGRYFSDASLEDNILNNVDTFWKQVPAQVEIIDMEGQVLMNSLGTVTDP
                     QTADVQSALQGTIATWIGRTATERVMAAAIPLQVEEEQIGVLRFIASLREVNAAIISI
                     AYKYFSFGGIVILFTGLVSLILANTITVPLNKVTQAAEIMAAGNFEVECAPHKLDEIN
                     KLSATLNYLAGEIVKKDALKNNFISSVSHELRTPLTSIKGWAVTLQQGYENIDQLQDG
                     LTIIEKESDRLTQMVTELLDFSKFISGKITLKIEKVHLPTLLKHLHLQLSLRASREEL
                     NFHVSIDQQTPYVCTDGNRLKQVLLNLLDNAFKFTPAGGTVALSSRTDGDVYTFTLQD
                     TGCGIPVEELPHIKEKFFKGKSSKAQNGIGLSLADEIIRLMQGKLEITSEVGRGTLIT
                     ITLPLKEVRAND"
     gene            7416..8834
                     /locus_tag="VFC74_02330"
     CDS             7416..8834
                     /locus_tag="VFC74_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24207.1"
                     /translation="MTKKAILLFLLLGILTVGFITSNSSYTLRLVPPLSPSPLQGTWK
                     ITSVLQENNATSEQWLGNTILFTNDYALLGNYLLPQPRFQLKKVTASSYIDINQTADS
                     GSYTHKEDLEFLSLSDHHLFYCELLPQKEDELILYLFNNSYLVRKISDTVDKSLLNSP
                     NITDITNITNPQQAHLQSGILLGLRSPNNEYRTLWLAADDRQLAPLLQTKAIVFPRRN
                     GFYELEVVALHQGQQREDFLLAKPLAGYKKKKEPQLSLDPLTWEDKVGSIQRKICYVG
                     NDFVSLEETIVQTAVNGSTSEVSKLLTLSVDSLPAIKAVKISDITKPGTMPALQTQVE
                     QVPLAAPLTEGNFSLARNKGYWQLIRRLANEPDLPLGIIPPSHILFYNELSIPWPRVK
                     NHVPQAEDVLTSPNEDLALVFTSNEIIVYAMFHQKFAATPLLKIPLLAGEELIMTEWA
                     LGHYVENWTLTMQSCSNLQSEA"
     gene            complement(9031..>10306)
                     /locus_tag="VFC74_02335"
     CDS             complement(9031..>10306)
                     /locus_tag="VFC74_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24208.1"
                     /translation="YMTERLLITTAAMGAILLVARIIDLIIGFLSGMIVQKTSFKFGQ
                     YRSWLLFGPISVAIGVIMCFLNPNLSIQMKGIVVLIGYILYGGGMSFGQIAQNGMMSK
                     IAGPNLDMRLTISGKIVQGQTVAQIATALTIMPLILWVEGFGIDGYTLVAILFSVISL
                     AAQTTLFRGTKEYDQYDPDFKSKGGASVSVGAMIGDILKNGQILLVMLGDTLRFAAMM
                     TIMSLAMYYFTFVAKNPLLISVAMLSQSIAGFTGALFAPSIVKKIGKKKAAVISGAIA
                     TSSYTALALFAGSNPILYIVLNSIAFLGMSLVNSCGVNLYLDCAEYQLYKTGKDNKTF
                     SMSMFGVAIKVGFAVTSILVSWVLQTAGYDAATGMAEPARLAKLIGAVPATLYLVYML
                     IYLFFYKITEDKSREYAAHNYQKAAAAQKANN"
BASE COUNT         3015 a         2388 c         2070 g         2833 t
ORIGIN      
        1 gccaataagc atgtttatat taactttata atcatacttc aaatttttac ccttattatt
       61 ttgtgccacc ttctcgttag cttcatttct cgcaagtgct gccatattag ataagtaaat
      121 tgatgcatag aaatcttgct caacagagat tgccgtatcc cctgtaaagt tttcaatttg
      181 caatttactt tttagttcat tgaatttgac ttctattccc cagcgtttaa aatatagctt
      241 tttgaagtca tgagtattaa ggctttcatc aaacaaatta gttactagca cttcttcttc
      301 tcctgaatct aatccaaatc taaggactct aacgtttagt ttcgggtgtc gcacttttac
      361 aagtaactca attacctgat cgggtttttg tgcctgatgt atttgcctga ttttctggct
      421 agatatgcgg cagacatact taattccgct tttatctaag tactcgaaga attcttttga
      481 aggatagccc ctatcaaata ggattaagtc attttttaac cctaactctt tgagcttctc
      541 aatgagttcg acggccattt ctctttcacc agtggtatat tttgaaatct tggatgtgat
      601 tatcatatcg ttttctatat catagatgct ggatgcttta gcacgggcaa gcttaactgt
      661 cttactacct tcactatagc cgtaagcgtc ccttaaacgt tgtgaattgt tgatctctaa
      721 tattgatgca tcaatagcac agagacggta ccctttgaat tttttaaaat tgtcgtcccc
      781 ataataccaa cttgtaattg catccgccat tttaataaat gctgtaggtg atatcttttg
      841 ccttgcttca gagtaacctt gcttcgtgat actcatagta cctcctttaa tcgttttgaa
      901 aaatgagtct agttctaact gaagagtctt ttttacaaag tttagctgaa ataagataag
      961 gcttttaaaa tccatcttgc agttagcagc acgagtaaaa taggttggct tagtacgtga
     1021 ttcacacata aatataatat cattgataag cctttctgta atgagtaacc ctttaaacaa
     1081 agtctttttt tctgtcatta tatcaccact tatcgatttg atataccaaa taatggtatc
     1141 caagtcaatt gtgggggctt ttcgaaaaag tcaagtccta atttcataaa ttaagacttt
     1201 tttataaaaa ggccaacatc cattaaatgg aatgttagcc tgatgacatt ggggttaccc
     1261 ctctacggtc aaaaatcctg gtaccacttt cacggcacca gcagaatgat gataatacta
     1321 tcttagtttg cgtaaagatg tttctgcggc aaaagttatc gtattgtaaa caaagaaaat
     1381 ctcttccagc cctttttctt caaaataatc ttttacttgg tggtgaaagt aacgtagatc
     1441 tagtacatgg atttcactaa aatgagggat taaaaacggc accaatgcat tggcgtagga
     1501 gtctttaatt atcagcagtt tcttccctgc ttgattgggc gtggtgattt taatcagggc
     1561 atggttacca tcgagaaaaa gtgcatattg atctttcttc tgtaaatgtt cacaggcata
     1621 tacactatca cttattttat gcttatctgc ataagcaaca gtatattgct gctttttctg
     1681 gggtacaaaa agctgaattg tatccggttg gcagggataa ccgctttggg aataaagagt
     1741 accataaaag ttgtcactca cttgttttac agtataagca tcttggggaa gtgcggttac
     1801 ccccattatt tttgctgctt cttgataggc aaaatacgcc cctctggttg tccaatgatg
     1861 gtccgttttg tagtaaatat tctcctgtcg ctgggactca agtacggcag tggggtcgat
     1921 aaaagtgatt tctttactca tttggctcct gacttcaccc ggctcgatga tttgcggtgc
     1981 ggcatacctt ggcagcttgt cccgcaaaat ctgcgccgat acgggagcca gcataaaata
     2041 gattctgccg tttacctgct tggcaaactc attgacagca gcaatattgc gccctacttg
     2101 ctctttttcc tgctgcgacg gcttttgcag caggtaaccc tctcggccaa aatacacacc
     2161 atgatgatcg tttttttgca gcagcagttc tgctttggcc ttcaaactaa tccaagtgtc
     2221 ccgtagaata aattggtcgc taagatattt ttctgcagtc tgaccaaatt caccagataa
     2281 cagcttgtcc caggagaaag ccggtcttga ctgcagatac ctgttttctg cctcagaaaa
     2341 agtacgcttg ggcagcacgg caaataataa acagaaaatg cctaaataca gcaccatcgt
     2401 aatggttaag gccttatgat agctttgcac aagctacccc ctctctaaaa gcgaaagtac
     2461 aaaaatgggt tataggtgga atcaactaaa taagctgtag tgagcatgag cagcagtatc
     2521 tgtgccgcca cagccgcagc ataagtccaa gctggcgaaa tattttcacc ccaacggata
     2581 gttttcttgc tccagggcac agaacaaagc acagctaatg ctaacaataa gccataatta
     2641 gttaacaagt acagcgtctg ctggtctagc caaccattac tgttgaaacc aaacatggcc
     2701 tgccaatact gacctgcctt aactaagtcg gtaaaaacaa agagcaccca ccctaatagt
     2761 attaaaacct tggcataaag atggcggagc agacaaggca gcagctgcag ccagcgccct
     2821 agaaatgcct tttccatggc taaaatgaga ccaaagtaaa gaccccagag cataaagttc
     2881 cagctggcac catgccataa gcctgttaaa caccaaacta ctaagagatt acggtacatt
     2941 aacctaggac cccggcggtt gccacccaaa ggaatataga catattcccg aaaccaagtg
     3001 cctaaagaga tatgccagcg ccgccaaaaa tctgtaatac tctgcgccag atagggataa
     3061 ttaaaattct ctaaaaaggt aaaaccaaac atctgaccta acccaatggc catgtccgaa
     3121 tagccgctaa aatcaaaata aagctgcaag gcgaaggcta tgatacccag ccaggcaccg
     3181 ctgatggtta actctgcagc gggtagttgc tgcacttcct gccacagtaa cccaagatta
     3241 ttggccagca atactttctt tgccaaacca attattaacc tgctgacccc gcggccaaag
     3301 ctgctgatgt cctctctgcg ctgcaatagt tgttcagcta ccgtttggta gcgcacaatc
     3361 ggcccggcaa ttaactgagg aaagagggag acataggtac caaaggcaat taggtttctc
     3421 tgggggagta cctcagctcg gtaaaggtca agcagataag agagggtctg aaaggtataa
     3481 aatgaaatgc ctatcggtag gggcaagttt agctcctgca gtgctgtacc aaagacaaag
     3541 ttaatgcttt ggcttattag atctgcatac ttaaagaaaa aaagtaaacc taaattgagg
     3601 gccaaggaag agtaaagaat aattttggcc agcttgggct gtttttcata tctggccagc
     3661 aaaagaccgt acagatagtt gataacaata gaaaataaca ttagccagat atagatgggc
     3721 tccccccaag cataaaaaat caaactcgct aaaaacaaag ctgcatttcg gcagctttgc
     3781 ggcagtagat aatagcttag taaaaccaag ggcaaaaaga gaaaaagaaa aaaaaggctg
     3841 ctaaaaatca tgatgtttac ccttcttcat ttccgtacag tagttgctct acttctttgc
     3901 taactttttc attgtgccca gagacacaaa atatcacata aggtccagtt acccgtagat
     3961 agggatcctc aagcttcggt agttccaccg gcagataatc ggcataactt gctttttgct
     4021 ctgctagtcg ctgcaaaaca acttctttaa gacgttctgc cgcggccgca tccacagctt
     4081 caaagacagc aatttcctct gctgtagccc cggtgctgac ataaacttgt tgcgcggcta
     4141 catctttttc atctacaggg taaagaacca tgctcatttg ttcatcaata gcactcaatg
     4201 tatcggtaaa gggaatcctt tcccatagca cctccgcaca ttccgctgcc tgcaaattaa
     4261 gcttgctgct ctggccgcag ccagcagcca gcagcatcag cagcatcacc cagcttaggc
     4321 atattttacg acgcatcctt aaccacccct cccgttgtcc caacaagata atactgctgt
     4381 aaataatcaa accacaattg acagtaggct ggattgagat gaatcccatc tgtagtggca
     4441 gcactgggca gatagccttc atcgtcttta acagcggcgg ccgcatccaa aaaataaacc
     4501 tgctgctctg cagccatttg ctgtatcagc tcattatatt tattgatgtt ttgctgattg
     4561 taaacattat cctgaaggga cttttttttc gttaccggca ggatagactg cacataaatt
     4621 agcgccgcgg gcgctatttt ttttagttcg gcaataactt ccccataggt cttaataaac
     4681 aagtcactgt aagcccagcc cagttcattg atgcccagca taatataaat cttgtcaaac
     4741 ttatgctggt ctagggcctc catgatggta atcttttgtt tgccgctcgc caccaccggc
     4801 ttggtagaaa tggtatcaac ctttaaacca gtggccgtat aataaatcgc ctctgtcgga
     4861 ccgccgaata actgcagtcc tgttgtccgt gaatcgccaa taaacacagc atcttgaaag
     4921 taatcttcag ctatggtgcc tgcagctgct ggcacagcac tatggaaatc ataggctgaa
     4981 gattgctgat ttggttcgcc cgctggcatc agcggcggct cgatttcttc tgtttgtgcc
     5041 actgccagct cctgtttgaa cccgggtgag aaataaaaca aaataatcaa cgagatgagg
     5101 acgagaacag taaagttgag tacttgccaa cgccctcgtt tagccgccat catctcacct
     5161 tccttccgcc cacttaacgt cctccattta ctgtacctga tttgcctcga taatgattta
     5221 caaatccttt aagattgttt acgaaaaggt tacagtttta agttggtagt taaaaacccc
     5281 taacaattcc tttgctttat agtaaaatag atatggacac taaacaaagg aggtaagttc
     5341 ttgactgcaa gcaaagtatt agttgtcgaa gatgaactat ccattcgcaa atttatcgct
     5401 ataaacctac atagaaataa ctttgaggtt ctggaagcag cagatgggga gactgccctg
     5461 actttagtgc aagcatactc ccccgaggtg gttattttag acatcatgct gcccaccatc
     5521 gatggctttg aagtctgcct gcaaatccgg caactgaggc cgcagactgt gatcattatg
     5581 ctgaccgcca aaggtgagga tttagataaa gttatggggt tggatcttgg tgctgatgat
     5641 tatatggtta aacccttcaa ccccttggag ctgatagcac gcatccgcgc ccacctgcgg
     5701 aaaataagcg ttcttccaca acaaagcaac tttttacact ttcggcagtt gaaattggat
     5761 ctggaaggtc aacgttttta taaagataag caaagcattg agttaacacc caccgagttt
     5821 gccatcatga agctgttcat gcagcagccg caaaaagcct tcacgcgtaa tgaaatcctt
     5881 aatagcatct ggggccagaa ctattttggc gacttaaaaa ctgttgatgt tcatattcgc
     5941 agaatccgag aaaaattaga ggacaaccct tctgacccgc aatggcttga aacagtctgg
     6001 ggattaggct atcggctaaa aggagacagt agtaatgcgc agtattaaac atagaatggt
     6061 gctttacttc atgttcatta ttatcattac cgtgattatc tttgagttct ttctcatcac
     6121 cagtctccgc aaaaactact atcaaagcct ggaagattct ttacttaacc agctgcagac
     6181 ttcggctgaa ttatatggac gttatttttc tgacgcctcc ctggaagata atattctaaa
     6241 taacgttgat actttttgga aacaggtacc cgcccaagtg gaaattatcg atatggaggg
     6301 tcaggtgctg atgaactcac tgggtacagt gacggacccg cagacggcag acgtacagtc
     6361 agctttgcaa gggacaatag ccacctggat cggacgtact gctaccgaaa gagttatggc
     6421 cgctgctatt cccctccaag tggaagaaga acaaattggc gtcctccgct ttattgcttc
     6481 actgcgggag gtaaatgccg ccattatcag tattgcctat aaatattttt ccttcggcgg
     6541 cattgtgatt ctctttaccg gtttagtcag tctaatcctg gcaaatacca tcactgtgcc
     6601 gttaaacaaa gtaacacagg ccgcagaaat tatggctgcc ggcaattttg aggttgaatg
     6661 tgcgccccat aaattagatg aaattaataa gctttccgcc acgctcaact atttagccgg
     6721 tgaaattgtc aaaaaagatg ctctgaaaaa caactttatt tcctctgttt cccatgagct
     6781 gcggacgccg ctcacgtcca tcaaaggttg ggcagttacg ctgcagcaag gttatgaaaa
     6841 tatagatcag ctccaagatg gacttaccat tattgaaaaa gaaagcgacc gactgacgca
     6901 gatggtcaca gaactattag atttctcaaa attcatttcc ggtaaaatca ccctaaaaat
     6961 agaaaaagtc catctgccca ctttgctgaa gcatctgcac ctgcagcttt ccctgcgcgc
     7021 tagccgcgaa gaattaaatt tccacgtaag tattgaccag caaacaccgt atgtttgtac
     7081 tgatggtaac cgtcttaagc aggtactgct taatctgctg gataatgctt ttaaatttac
     7141 tcctgccggc ggtacagtcg ctctgtcttc ccgcaccgac ggggatgttt acacctttac
     7201 cctccaggat accggctgcg gcattcccgt ggaggagcta cctcacatta aggaaaagtt
     7261 ctttaaaggc aaatccagca aagcacaaaa tggcatcggc ctctcgcttg ccgatgagat
     7321 tattcgtctg atgcagggta aacttgaaat tacaagtgaa gtaggtcgtg gcaccctgat
     7381 cactattact ttacccttaa aggaagtgcg cgctaatgac taaaaaagca attcttcttt
     7441 ttttgctgct ggggatcctc acggttggct ttattacctc caacagcagc tacactctcc
     7501 gtctggtgcc gcctctcagt ccatcacctt tgcagggcac ctggaaaata acctccgtct
     7561 tgcaggaaaa taatgcaaca tctgaacaat ggcttggtaa taccatcctt tttaccaacg
     7621 actatgcact gctggggaac tatctcttac cccaacctcg tttccaatta aaaaaggtta
     7681 cggcctccag ctacatagac ataaatcaaa ctgcagatag cggcagctac acgcacaaag
     7741 aagatctgga attcttatct ctgtctgatc accatctctt ctattgtgaa ttactacccc
     7801 aaaaagaaga tgaacttatt ctctatcttt tcaataatag ttatctggta cgtaaaattt
     7861 cagacacggt cgacaagtcc ctcttaaact ctccgaatat taccgatatc accaatatca
     7921 caaacccaca gcaggcacat ttacagtctg ggattctact tggcctgcgc tcaccaaata
     7981 acgagtatcg taccctctgg cttgctgctg acgatcgtca gttagctcct ctcttacaga
     8041 caaaggctat cgttttcccc cgcagaaacg gtttttatga gctggaagtt gtagccctcc
     8101 atcaaggtca acaaagggaa gatttcctgt tagccaagcc tcttgccgga tataaaaaga
     8161 aaaaagagcc gcagctctcc ttggatcctt taacttggga agataaagtt ggctcaattc
     8221 agcgtaaaat ttgctatgtc ggcaatgatt ttgtttctct ggaagaaaca attgtccaaa
     8281 cagcagtaaa tggcagtacg tctgaggtaa gcaagctgct aacactatct gtagatagct
     8341 taccggccat taaagcagta aaaatatctg acatcacaaa gcctggtacc atgcccgccc
     8401 tgcaaacaca agttgagcaa gtacccctag cagctccttt aactgaagga aatttctcgt
     8461 tggccagaaa taaaggttac tggcagttaa tcaggcgttt agcaaacgaa ccagatctcc
     8521 cccttggtat catcccgccc agccatatcc tcttctacaa cgagctctct atcccctggc
     8581 cccgcgttaa aaatcatgtc ccccaagctg aagatgtctt aacttcacct aatgaagatc
     8641 tcgccttagt ttttaccagc aacgaaatta tcgtctatgc catgtttcac cagaagtttg
     8701 cggccacacc actattaaaa atccccttgc tggcaggaga agaactaatt atgactgaat
     8761 gggcgcttgg tcactatgtt gaaaactgga cactaaccat gcaaagttgc tctaatttgc
     8821 aatcagaggc ctgagtgcat ttttacagga agagatcttt tctttagcaa gataaaattg
     8881 ccagataaag tttacataca aaagtgccgc ggcctatgtc cataggcgcg gcacttttat
     8941 tacatcttca gctagtgctc actagcaata agcatagtta gtacattaat gataggatct
     9001 ggccggcagc gtgcgcctgc taactagcta ttagttattg gctttttgtg cagctgctgc
     9061 tttttggtag ttatgagcag catactcacg ggatttgtct tcagtaattt tatagaaaaa
     9121 caagtaaatc aacatgtaga ccaagtaaag cgttgccggc accgcaccga tcagcttagc
     9181 cagacgcgct ggctcagcca taccagtggc ggcatcgtaa ccggctgtct gcagcaccca
     9241 agaaaccaaa atcgaagtta ccgcaaaacc aactttaatc gccacaccaa acatagacat
     9301 ggagaaagtt ttattatcct tgcctgtttt gtatagctgg tactcggcac agtccagata
     9361 gaggttgact ccacaggaat taaccagaga catgcccaag aaagcaattg aattcaagac
     9421 aatgtacagt atcgggttgg agccggcaaa aagcgctaag gccgtgtatg atgaagtggc
     9481 aatggcaccc gagatcacag ctgccttctt cttgccaatt tttttgacga tagacggcgc
     9541 aaatagcgcg cccgtaaagc cggcaatact ttgtgaaagc atcgccacgc tgattagcag
     9601 cgggttctta gcaacgaaag tgaaataata catggccaaa gacatgattg tcatcatggc
     9661 ggcaaaacgt aaggtatcgc caagcataac tagcagaatc tgtccgtttt taagaatatc
     9721 cccgatcatg gcgccaacac tgacactggc tccacccttg cttttgaagt cgggatcgta
     9781 ttgatcatac tcttttgtgc ccctaaataa tgtcgtttgt gctgcaagac tgataacgga
     9841 aaagaggatt gctaccaagg tataaccatc aatcccaaag ccttccaccc agagaataag
     9901 gggcataata gtaagcgcag tagcaatctg agcaacagtc tgaccttgaa caattttacc
     9961 cgaaatcgtc aaacgcatgt ccaggttagg accggcaatt ttagacatca tgccattttg
    10021 agcaatctgg ccaaaactca tgccaccgcc gtaaagtata taaccgatca gcacaacgat
    10081 ccctttcatc tgtatagaga gattgggatt aagaaaacac ataattaccc caatagccac
    10141 actgatcgga ccgaagagca gccaagaacg gtactgtcca aatttaaatg atgttttttg
    10201 tacgatcata ccggaaagga aaccaatgat caagtcaata attctggcta ccaaaagaat
    10261 cgcgcccatg gcagcagtgg tgatcagtaa tctttccgtc atatag
//
LOCUS       DASRMS010000049         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_413789, whole genome shotgun sequence.
ACCESSION   DASRMS010000049 DASRMS010000000
VERSION     DASRMS010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_413789"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="VFC74_02340"
     CDS             complement(<1..967)
                     /locus_tag="VFC74_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24209.1"
                     /translation="MEKVLITDNFAVKYDSGFYLQPLNIVLNKGEIAAVVGESGSGKT
                     TLAKALLKLPASQASCEGEVVINGVNIYQLSAAELLARRSVEFAICLQNSSELLNPLL
                     TIREQLYEVLRKAVPEAELELKATWLLAQLELTPEVLEQYPRQLSGGMLQKVLIICAV
                     ALSPSLVILDEPTSSLDERAKSRVLDLINMLRQRYQTTFLIVTHDLSVAKAISQRMFV
                     LYQGKLVETGLTTSLLNKPKHPYTRGLIDSSIEINPYRDLWGIRQVAAAESEKSGCPF
                     YPRCTQRLPHCAQQQPDLAPHHSEPERLIACNRGGVITVLAGKMIT"
     gene            complement(969..1781)
                     /locus_tag="VFC74_02345"
     CDS             complement(969..1781)
                     /locus_tag="VFC74_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24210.1"
                     /translation="MSKQLQMYLLVLIVMTIIFTYGSFIYPHSATSPDGASLQKPSTV
                     HLLGTDDLGIDIFAQISRGFFLSLLIGAATAVLSLLLGGSLGVAAGYLGGRVDDLLSF
                     ILNLFLSLPQLPVMIVLGAFFGQSTWHVIVIITLFSWAAIAKIVRAKTMQLRSNAYIT
                     LARSYGGGFWYIFSRHLSAEILPLLLINSLAVVGKAIIQEASLAFLGLSDPLAKSWGL
                     MINKAMNFGGIYYTDYWQWWLLAPLFSLVATIYCLRMLAWEMEKEIIGKERV"
     gene            complement(1783..2736)
                     /locus_tag="VFC74_02350"
     CDS             complement(1783..2736)
                     /locus_tag="VFC74_02350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007491821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24211.1"
                     /translation="MSRKLLLLVCAFLVIYTFNFFLPRLMPGDPFAHNTAMSGQEMQG
                     LSKAELARLKAYYGLDKPLWEQFKKTIKDNLQFNFGQSILYKKSVAAIITSRLPWTLY
                     IMVSTLIISLLLGVLLALLCLQNSQWDTLVYKGMSLLAEIPAYLVGILLLFLVAARVK
                     AIPLAGNLTPFYQHPNSFSWLVDVVKHSLLPISALVITMMPSFYFTARTSMQSIMTKE
                     YVLNAYAKGLSAKTIRYKYVLLNAIFPLAARFFLNVGHCIGGTMLIENVFAYPGLGKV
                     LRDAVMYRDFILIQGVFLLSTTVVLISSFISDLINYYLQKG"
     gene            complement(2793..4346)
                     /locus_tag="VFC74_02355"
     CDS             complement(2793..4346)
                     /locus_tag="VFC74_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK24212.1"
                     /translation="MSKKMLAVILLVISLFTLAACGESVINKVGDEQLQTLKLAGGTD
                     WGAPSPYLHDPRGPGTAKMKLVFDSLLDADAQGDIPWLAKEWEIKGNAYTFTLSEQAT
                     FHDGQPVTTADVAFTLDYYQKFPPVTNYLDYNGHSIVASYQIIDEQTITLTVEQPLAT
                     TLVKLGSFVILPQHIWENVADPYTFNEAAAYIGSGPYTFGAYDPAVGSYEFIAYDNYY
                     GHQPVAARVLFVPVSEPLLAFDHGEIDLTEVPIDALQQYQDDPTIGMLGKENDMGYKM
                     LVNMEKLPEFKELELRIALYQALDRQEVVAKVFRGSGAVGSAGYVPPTNYFYSERVIQ
                     YDYQPTRAKKVLAKKNLNVELLVANSSDDLKIAELLKHDFEAAGMQIKVVALDAKIRD
                     ERILAGEYELAVVGNGGWSRTPDYLRTLYSSQAKYTGSNPHFMGARGHDNQIITSLAE
                     AQLLELDFDKRQEIFADLQYEISKEVPIVVIATKTTQVIFKKDYYSGWVKTYDYQQVE
                     QNRLSYVEK"
BASE COUNT         1349 a         1082 c          915 g         1231 t
ORIGIN      
        1 tagtaatcat tttaccagct agaacagtga ttaccccacc gcggttgcag gcaatcaggc
       61 gctcaggctc actgtggtgc ggggctaaat ccggctgctg ctgagcacaa tgcggcagac
      121 gctgtgtgca tctaggataa aatggacaac cactcttttc actttccgct gctgccacct
      181 gacggatacc ccatagatca cgatagggat tgatttctat actggaatca attaatcccc
      241 tggtataagg gtgcttaggt ttgtttaaaa ggctagtggt aagacctgtt tccaccagtt
      301 tgccttggta gagcacaaac attctttgac taatcgcttt agctacgctt aagtcatggg
      361 taacgatgag gaaagtagtt tgatatcttt gcctaagcat gttaatcaag tcgagcacac
      421 gacttttcgc tctttcatct aaagagctcg ttggttcatc taaaattacc aaactaggtg
      481 acaaagctac tgcacaaata atgagcacct tttgcagcat gccgcctgat aattgccttg
      541 ggtactgctc aaggacttca ggtgtaagct caagctgcgc taaaagccat gtggccttta
      601 actcaagttc tgcttctggt accgctttgc gcagcacctc ataaagctgt tcccggatag
      661 tcagcaaagg attcaatagt tcactggagt tttgcagaca gatggcaaat tctacagaac
      721 gtctcgccag cagctctgct gccgagagtt gataaatatt tacaccatta atcacgacct
      781 ctccctcaca tgaggcctgg ctggcaggca gcttcaggag agctttagcc aaggtcgttt
      841 ttccactgcc cgactcacca actaccgccg ctatttctcc tttattaagc acaatattta
      901 gtggctgtaa ataaaaccca ctgtcgtatt tgactgcaaa attatctgtt atcagcactt
      961 tttccatctt ataccctttc tttacctata atctcttttt ccatttccca cgccagcatg
     1021 cgtaaacaat aaatagttgc cactaaagag aacagcggtg ctaacagcca ccattgccag
     1081 tagtcagtat aatagatgcc tccaaaattc attgccttat taatcatcaa gccccatgat
     1141 ttggccaaag ggtccgacaa acccaaaaag gccaaggagg cttcctggat aatggctttt
     1201 cctaccacag cgagagaatt aattaagagc aagggcagaa tctccgcgga caaatgtctg
     1261 gaaaaaatgt accagaaccc tccgccataa ctccttgcca gtgtaatata agcgttactt
     1321 ctgagttgca tagtttttgc ccgcacaatt ttggcaatgg cagcccagga aaatagcgtg
     1381 ataatcacaa ttacatgcca cgtactctga ccgaaaaatg ccccgaggac aatcattacc
     1441 ggcaattgag gcaaagataa aaataaattc aggataaaac taagcagatc atccaccctg
     1501 ccccccagat atccagcagc gacacctaaa ctgccgccta atagtaagga caaaactgcc
     1561 gtcgctgcac caatcaacag agacaagaaa aagccccgcg agatttgggc aaaaatatca
     1621 atacctaaat catctgtgcc taataaatgt acagttgatg gtttctgcag cgaagcacca
     1681 tcgggactag ttgcactatg ggggtagata aagctgccat aggtaaaaat aatagtcatg
     1741 acgattaaaa ctagtagata catttgcagt tgcttgctca tattacccct tttgtaaata
     1801 atagttgata agatcggaga taaaagaaga aataagcact acagtagtgg agagcaagaa
     1861 tactccctgg ataagaataa aatcacgata catgacggca tcgcgcaata ctttacctaa
     1921 gccaggataa gcaaaaacat tctcgattaa catcgtcccg ccaatgcagt gacccacatt
     1981 aagaaaaaat cttgcggcaa gcggaaagat ggcatttaaa agtacatact tataacgaat
     2041 ggttttcgca gaaagacctt tagcataagc attgagcaca tattctttcg tcatgatact
     2101 ttgcatactg gtacgtgctg taaaataaaa agaaggcatc atggtgataa ccagggcgct
     2161 gatgggcagc agactgtgct tcaccacatc caccagccaa gaaaagctat ttgggtgttg
     2221 ataaaatggc gttaagttcc cggctaaagg tattgcctta accctcgctg ccaccagaaa
     2281 taaaagcagt atccccacca gatatgcggg tatttcagca agcagagaca tacccttata
     2341 aaccagcgtg tcccactgtg agttttgcag acacaacagc gccaacagca cacccagcag
     2401 caagctaatg attaaggtac tcaccatgat ataaagggtc catggtagtc tagaagtgat
     2461 aatcgccgcg acactttttt tataaagaat actctggccg aaattaaact gcagattatc
     2521 cttaattgtc ttcttaaatt gttcccacag tggtttgtcc aaaccataat aagccttcaa
     2581 gcgagccagc tcggccttag ataaaccctg catctcctgc ccggacatgg ctgtattatg
     2641 ggcaaaagga tctccaggca ttaagcgcgg caggaaaaaa ttgaaggtgt aaatcaccag
     2701 gaaggcacaa actaaaagaa gtagttttct tgacatcatc atcctcccga aagcagtgtc
     2761 atagagggcc acgcccgcta tgacactgct ttttattttt ctacatagga aagtctattc
     2821 tgttccacct gttgataatc gtaagttttt acccaaccgc tatagtaatc ttttttaaat
     2881 attacctgcg tagtcttggt ggcgatgacc acaattggta cttccttact gatttcgtac
     2941 tgcaggtcag caaaaatttc ttgacgttta tcaaagtcaa gctccaaaag ttgagcttct
     3001 gccaatgacg tgataatttg attatcatgt ccgcgtgccc ccataaagtg agggttggaa
     3061 ccggtatatt tcgcctggga ggaatatagt gtacgcaaat agtccggtgt acggctccag
     3121 ccaccattac ccaccactgc aagctcatat tcaccagcca gtattctttc atctctaatt
     3181 tttgcgtcca gagctaccac ctttatttgc ataccggcgg cctcaaaatc atgcttcaac
     3241 agttcagcta ttttaagatc atcgctacta ttagccacca aaagctccac atttaagttc
     3301 ttctttgcca agactttttt tgcccgcgtg ggctgatagt catactgaat tacccgttca
     3361 ctgtaaaaat agttggtagg aggcacataa ccggcactac caacggcacc actaccgcgg
     3421 aagactttag ctactacttc ctgacgatca agagcctgat acaaggcaat gcgcaattca
     3481 agctctttaa actccggcag tttctccata tttaccagca ttttgtaacc catgtcattc
     3541 tctttaccca gcattcctat ggtcgggtca tcttgatatt gctgcagggc atcaattggt
     3601 acttcagtaa gatcgatttc cccatgatca aaagccagca gcggctcact gacggggaca
     3661 aaaagtactc tggcagcaac aggttgatgg ccatagtagt tatcgtaagc aataaattca
     3721 tagctgccga cagctggatc ataagcgcca aaagtatagg gaccagaacc aatataggcg
     3781 gcggcttcgt taaaggtata gggatcggct acgttttccc agatatgctg cggcaaaatt
     3841 acaaaactac ccaacttaac taaagtcgtt gccagtggtt gctctactgt taaggtgatt
     3901 gtttgttcat caataatctg atagctcgct acgatgctgt gaccgttata atctagataa
     3961 tttgtcactg gcgggaactt ttggtaatag tcaagcgtaa aagctacatc agctgtggtc
     4021 actggctgac catcatgaaa agttgcttgc tcgctaagcg taaaagtata ggcattgccc
     4081 ttgatttccc actcctttgc tagccaggga atatccccct gggcgtctgc atccaacagt
     4141 gaatcaaata ctaatttcat ttttgctgtg cccggacctc gggggtcatg taagtagggg
     4201 ctgggtgcac cccaatcggt gccgccagct aactttaaag tctgcaattg ctcatcacca
     4261 actttgttta tcacactttc gccacacgcc gcaagtgtaa agagagaaat cacaaggaga
     4321 ataactgcca gcattttttt actcatcacg aaaaactcct ttacgaaatc attgactgat
     4381 aattgacaac agtgttacta attttatttt cgtgtcacct tactttattc tcgcttatat
     4441 ttttatttcc tgcttaaaat cagtcacaaa aacaaaaaag acgcatcatt tttttaagga
     4501 tgagcctttc atgttctttt gggggttacc cccaatgtca tcaggctatc tttcaaataa
     4561 ctgggaaaat gttaaat
//
LOCUS       DASRMS010000050         1309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_414431, whole genome shotgun sequence.
ACCESSION   DASRMS010000050 DASRMS010000000
VERSION     DASRMS010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414431"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1309)
                     /locus_tag="VFC74_02360"
     CDS             complement(<1..>1309)
                     /locus_tag="VFC74_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24213.1"
                     /translation="LLPELIPAAVDAVMTIVGALIENLPLLIEAAVELVSALVEGIGQ
                     ALPELIPAAIEAVITIVQGLLDSMDQVLEAAFAIIEGLAQGLLDALPQLIDALPAIIT
                     GIIDFITQNLPLIVAMGIELVIQLSTGLIEAIPHLVASLPQIVAAIITGIGNAAISIV
                     EVGKNIVLGLWDGIASMISWVKDKISGFVGDIVSGIKGFLGIRSPSTVFADMGANMAL
                     GMGQGFEENMSEVEKGMENRIPTDFEDTWNELSSNAVDSWSKITVSAQDIWTKLELFF
                     NETWKKTNENFNATWRGINDFAAKEWSSIRANANAVWTALSDFFNKNLASTKLSFETV
                     WNDTARIAESIWQAIKIQASSIWNAISDFYNTSWLNTKASFEAIWHGIRDTFISIWTG
                     LKTSAITLFNDVVNSIKNAFNIDWWSVGRSVIDGITRGVMDTAR"
BASE COUNT          361 a          321 c          248 g          379 t
ORIGIN      
        1 ttcttgctgt atccataacg ccccttgtta tcccgtcaat cacacttctt cccacactcc
       61 accagtcgat attaaaggca tttttaatac tgttgactac atcgttaaaa agagtaatcg
      121 cactggtttt aagccctgtc caaatgctaa taaaggtatc tcttatgcca tgccagatag
      181 cttcaaagct agccttggta tttaaccagc tggtattata gaaatcacta atggcattcc
      241 agatgctact agcttgaatt ttaattgctt gccagattga ttcagcaatc cttgcggtat
      301 cattccatac tgtctcaaag ctgagttttg tactagcaag gttcttatta aagaagtctg
      361 atagggctgt ccagacagcg ttagcattag cccttatact tgaccactct ttagcggcaa
      421 aatcatttat tcctctccat gttgcattga agttttcatt agtctttttc caagtttcat
      481 tgaaaaataa ttctagcttt gtccagatat cttgggcact aacggtgatt ttactccagg
      541 aatcgacagc attgctgctg agttcgttcc aggtatcttc aaaatcagta ggaatcctgt
      601 tttccatgcc tttttctact tcactcatgt tctcttcaaa accctggccc atgcctagag
      661 ccatatttgc ccccatatcg gcaaagaccg tagagggaga gcgaatgccc aagaagcctt
      721 taataccact tacaatatcg cccacgaagc cgctgatttt atctttaacc caactgatca
      781 tggaggctat cccatcccat aggcctaaga cgatgttttt accaacctct acaatggaga
      841 tagcggcatt accgatacct gtaataatgg cagctactat ttggggcaga ctagctacta
      901 aatgaggtat tgcttctata agtcctgtac ttagctgaat aacaagctca atacccatag
      961 caacgattaa ggggaggttc tgggtaatga agtctatgat tccggtaata atggcgggca
     1021 gtgcatcaat aagctggggt aaggcatcta aaagcccttg tgcaagcccc tctataatgg
     1081 caaatgcagc ttcaagaact tgatccatgc tatctaaaag cccctgcaca atggttatta
     1141 ctgcttctat tgcagcagga ataagctccg gcaaagcctg accgatgccc tctacaagag
     1201 ccgataccag ttccactgcc gcctcaatca aaaggggcag gttttcaatc agtgccccta
     1261 caatggtcat caccgcatca accgccgccg gtattagttc cggcaaaag
//
LOCUS       DASRMS010000051         1050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_750359, whole genome shotgun sequence.
ACCESSION   DASRMS010000051 DASRMS010000000
VERSION     DASRMS010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_750359"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..251)
                     /locus_tag="VFC74_02365"
     CDS             complement(24..251)
                     /locus_tag="VFC74_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24214.1"
                     /translation="MYLIGIKKDIHPCGNSGTLITRYKAEYKIRETAQRFAAMYSLTE
                     VYGLQAYPQRLCEMDGAAFVDYIRNNCKRYA"
     gene            complement(255..488)
                     /locus_tag="VFC74_02370"
     CDS             complement(255..488)
                     /locus_tag="VFC74_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24215.1"
                     /translation="MITTKEAARLRRSIKKLTACGGDCKNCKHAQIKTAWRGPNGYYA
                     FYCDVDCNIQPYSNTVRELRDITLDALKFELGG"
BASE COUNT          251 a          259 c          205 g          335 t
ORIGIN      
        1 ggcttgcatc ctgcggcctt tagttatgcg tatcgcttgc aattgttgcg tatgtaatcc
       61 acgaaggcgg ctccgtccat ctcacacagt cgctgcgggt atgcttgcaa gccgtaaacc
      121 tccgttaacg aatacatagc tgcgaatcgc tgcgccgttt ctcttatttt atattctgct
      181 ttatagcggg ttataagtgt tccgctgtta ccgcacgggt gaatgtcttt tttgatacct
      241 attaaataca ttgcttaacc tcctaattca aattttagcg cgtccagcgt tatgtcgcgc
      301 agttcgcgca ctgtgttgct gtaaggctgt atgttgcagt caacatcgca gtaaaatgca
      361 taatagccgt tcggaccgcg ccacgctgtt ttaatctgtg catgcttgca gtttttgcaa
      421 tctccgccgc aggccgtcag cttttttata ctccgccgca gccgcgcggc ctcttttgtt
      481 gtaatcatcg ctctgatccc cctatataat cgcttattag ttttttaatt ccgcgctgcg
      541 tgtcggccat taagcggcca tcgcgtgtat atgtactgta atatccttgc ggccatgttc
      601 gagttataaa atggtctttg tacatgtaat agctgttctt atcctccggc cgtgttacgt
      661 ctttcatttt agcttccccc taaaaaaaat gttattgtcc tgctctaaga ctatatatag
      721 tataccatag tatggactac cttgtcaacg tttattttat aattattgta attactttct
      781 cgcattatat cgtatcttat attgcgagtt tatgcttaaa atcctgggga acctggggaa
      841 cctgatctcg gaacctgatc tcggaacctg atctcggaac ctggggaacc tgatctcgga
      901 acctggggaa cgcccaaaaa aactattctg gtaaggacgt cattacaact cccttacccc
      961 actcagacaa atttttcatt aacactcaat ccaaagctta aactaaaagc ccaatccaaa
     1021 gcctaaacta aaagcttata atcagcgcaa
//
LOCUS       DASRMS010000052         3651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_213669, whole genome shotgun sequence.
ACCESSION   DASRMS010000052 DASRMS010000000
VERSION     DASRMS010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213669"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="VFC74_02375"
     CDS             <1..680
                     /locus_tag="VFC74_02375"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK24216.1"
                     /translation="FILPSSTLPPAKVGKREIEFHQENLIVIGTNVDFLCTTHAPTRQ
                     YTNITIQKEFFEQIANEVIGKREVDFSKVKFFGSPQLLRIIADLEFELTYFNNDCAQM
                     VDSIATQMIIQLLRDTNDYTHHSKSNKRQGQVPLLRTVVNPMYFVVLTSCNNLFPSGS
                     KLTSREIEIAAYLLDRFDYVSIAESLMVSPNTVKTHVKHIYDKLKVAGRKELSHKIIE
                     LYQDCLR"
     gene            1046..1357
                     /locus_tag="VFC74_02380"
     CDS             1046..1357
                     /locus_tag="VFC74_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24217.1"
                     /translation="MTGLTSKEELITLTFYDSENTNILEVRFYNSSFYEQEVGDNKHY
                     TPVPDLGDQAAIAIPDMPYRVTFLQGEHSIMMQSIPVDGDVPLTEEQLIQLAKIVVSR
                     L"
     gene            1469..>3651
                     /locus_tag="VFC74_02385"
     CDS             1469..>3651
                     /locus_tag="VFC74_02385"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24218.1"
                     /translation="MAETTVTDEAALRAAVTAADAGDIITIGADITLTSPLTLLPVVA
                     RAEVLPLDRSEETADSGLSVDTDEDMDTDADANVGAQRLPPTSDMIPMSINPEHAYVV
                     DENSYATLGEALNAVPSGGTGTIELRATVIHNSPVEISNKNITFELTQGSLTIDVSSG
                     TALTVSLGSVVTLTGSGTLDVKGPAGGVYAEGATVTVDNALATSGTGAYARDGGVITV
                     LGNTTGSSPGAFAFGSGSIITVEGDAKTTGSGANAVFAMSGGRVEAKNAIATGTNSNG
                     VQASAGTVLISENVRGNNYGAWAEEKGVITIGGDVTADGTNSIGAWAYTGGTITIDGT
                     ITAINYIRIGSSIKTADQITTPTTLTGYYTYTDGTSTVWVQDTGLLDPEDVCKIGTVG
                     YPTLEAALAVVENNETITLLQTIDHFKPIEINGKTITFDLGSFDLNIDTSGTAASYAL
                     TAKNGGKVICTGSGKMNVKGLKYGVRVEGGSEIHVSGNVIAGQYGISTVSSGSGNPKI
                     TVDGDVTATGQGVDADGEIEAIGAGGYATVEIGGNVTANRISYNQIVQAVYANGSTIT
                     VGKNVITQGSGINVQNNGNVTINGSLDFNPTGTGSQWYIKLGYPVVIKTSDDYEDTST
                     KPGYKEYKNGDNIVWIKGAIQTTYAVTVIGGTGSGNYAENATVNITAGTAPEGKEFDK
                     WTTTDGVTFANANSQSTSFTMPDKAVTVTATYKDLAPSYYSVVVQ"
BASE COUNT         1072 a          840 c          866 g          873 t
ORIGIN      
        1 attttatttt gccttcatct acattaccgc cagctaaagt tggaaagcgg gaaattgagt
       61 ttcaccaaga aaacctaatt gttatcggta ctaatgttga ttttttatgc acgacccatg
      121 ctcccacacg tcaatatact aacattacta ttcaaaagga attttttgag caaattgcca
      181 acgaggtcat tggtaaaagg gaagtggatt tctccaaggt aaaatttttc ggcagccccc
      241 aattacttcg tataattgct gatctggaat ttgaactaac ctattttaac aatgattgtg
      301 cgcaaatggt cgacagtatc gccacgcaaa tgataattca attactgcgc gacacaaatg
      361 actatacgca ccatagcaaa agcaataaac ggcaagggca ggtgcctctt ctgcgtactg
      421 ttgtaaatcc tatgtacttt gttgtcctca cttcttgcaa taacttattt ccgtcaggtt
      481 ccaaactaac atcaagggag atagaaatcg ccgcttattt gcttgacagg ttcgattatg
      541 ttagtatcgc agaaagtcta atggtttcgc ctaacactgt taaaacccat gtaaaacaca
      601 tttatgataa gcttaaagta gctggcagaa aagaactaag ccataaaatt atcgagttgt
      661 atcaagattg cttacgttag tcagaaataa ctcatcaccc taagcgagca gaaatgctac
      721 ttttagggtg attttatttt ctcctataga tattaaaatg taattaaata cctaaattca
      781 ccactgttat aacttaacaa ccttcgatgc tgcgagttga tatcaaagga aggggagatg
      841 tatttgtttt aaaaaaatct tggttttaag tttcttaagt ttattattat ttttaacttt
      901 ggttggctgc gggaaggggg aaaaccagcc gccagcaaat tcaggcagca atccgcctgc
      961 ttcggaccca gtagatccat ctgcgcagac cacggacagg tacagcaaat atcttaccat
     1021 ctctgatctg caggaagtaa caggcatgac aggtctcacc tcaaaagaag aactaataac
     1081 cctcactttc tatgacagtg aaaatacgaa tattcttgaa gtacgatttt ataatagttc
     1141 attttatgaa caagaagttg gtgacaataa gcattatacc cctgtgccag acctcggcga
     1201 ccaagccgcc atcgctattc cagatatgcc ttatcgtgtg acctttttgc aaggtgagca
     1261 cagtattatg atgcagtcta tccctgttga tggcgatgtg cccctaacag aggagcaatt
     1321 aattcagctg gcaaaaatag ttgtttcacg cctgtgataa aaatatcccg gagaaaggaa
     1381 gaaacttatg caacggaaaa taagcaatgt ccatttaatc ctgctcgtga gtttgctcat
     1441 ttttgcattt atattgcccg gcgttgaggt ggcagaaacg actgtaacag atgaagccgc
     1501 tttacgagcg gccgtcaccg cagccgacgc cggcgacatc ataacgattg gtgccgatat
     1561 cacacttact tccccgctga ccctgctgcc tgtcgttgcg cgcgcagagg tgttgccttt
     1621 ggacagaagc gaggagacag cggattcggg cctatccgtg gacacagatg aagatatgga
     1681 tacggatgcc gacgcaaacg tgggtgcaca aagattgccg ccgactagcg acatgatacc
     1741 catgagcatt aaccccgaac acgcctacgt tgtcgacgag aacagttatg ccaccttggg
     1801 agaagctctg aacgcagtac caagcggtgg gaccgggacc attgaattga gggctacagt
     1861 catccataat tcacctgtag aaatttctaa caaaaacatc acctttgagc taacccaagg
     1921 gtcgcttact atcgacgtct cttccggcac cgcgctgaca gtgagccttg gttcggtcgt
     1981 cacgctgaca ggcagcggaa ctcttgatgt taagggacca gcgggaggcg tgtacgcaga
     2041 gggagcaact gtcacagtgg ataatgcctt agcgaccagt ggaaccggcg catacgcccg
     2101 agacggcggg gtcattaccg tgttgggcaa cacaacggga tcttccccag gtgcttttgc
     2161 cttcggttca ggcagcatca ttacagtaga gggcgatgcc aagactacag ggtcgggtgc
     2221 caacgcggtg tttgccatga gcggcggacg tgtggaggca aaaaacgcca ttgctaccgg
     2281 gacaaacagc aatggtgtgc aagcctccgc tggcacagtc cttattagtg aaaacgtccg
     2341 aggcaacaat tacggcgcct gggccgagga gaagggcgta attaccatag gtggagatgt
     2401 caccgccgac ggcaccaaca gcatcggcgc ctgggcctac accggcggca caattaccat
     2461 tgacggcacc atcacagcaa ttaactatat taggatcggt agcagcatca aaactgccga
     2521 ccaaataacc acccccacca cgctgacggg ctattacacc tatacggacg gaaccagcac
     2581 tgtctgggtg caggataccg ggttactgga tccggaggat gtctgtaaaa ttggcacagt
     2641 gggttatcct acgctggaag ctgcccttgc cgtcgtggaa aacaacgaaa ccatcaccct
     2701 tttacaaacc atcgatcatt ttaagccaat tgagattaat ggcaagacaa taacctttga
     2761 tctgggcagc ttcgacctga acatagatac gagtgggaca gccgcttcat acgcgctgac
     2821 ggcgaaaaac ggtgggaaag taatttgcac cggcagcggc aaaatgaatg tcaaaggatt
     2881 aaaatacgga gtgagagtag agggaggaag cgaaatccat gtcagcggca atgttatcgc
     2941 tggacaatat ggtatatcca ctgtcagcag cggctcaggc aatcccaaga taactgtaga
     3001 cggtgacgta accgcaacgg gtcagggcgt agatgctgac ggtgagatag aggcaatcgg
     3061 cgcgggtggt tacgctactg ttgaaatcgg tggtaatgtc acagcgaacc ggatatcata
     3121 taatcagata gtacaagccg tttatgccaa tggaagtacg attaccgtcg gcaagaatgt
     3181 aatcacgcaa ggcagcggca taaatgtcca aaataatggt aatgtcacca ttaatggttc
     3241 actggatttc aacccgacag gcactggttc tcaatggtat attaagcttg gatatcctgt
     3301 tgttatcaag acatccgacg actatgaaga caccagcaca aaaccaggct acaaggaata
     3361 taagaatgga gacaatatcg tttggataaa aggcgccata cagactacct atgcagttac
     3421 tgtaattggc ggcacaggca gcggcaatta tgccgagaac gcgactgtca atattacggc
     3481 tggaacagcc cccgaaggca aagagtttga caaatggacg accacagacg gggtaacctt
     3541 tgccaatgcc aattcccaaa gcactagttt caccatgccc gacaaggctg ttacggttac
     3601 tgcaacctat aaggatttgg caccttcata ttactctgtc gtcgtacaaa a
//
LOCUS       DASRMS010000053         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_482667, whole genome shotgun sequence.
ACCESSION   DASRMS010000053 DASRMS010000000
VERSION     DASRMS010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482667"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VFC74_02390"
     CDS             complement(<1..1284)
                     /locus_tag="VFC74_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HZK24219.1"
                     /translation="MQILDNVNQLFGEDLKTALTPNSKLKIAASCFSIYAYAALKREL
                     NSVESLEFIFTSPTFVPEQATDKLKKEQREFYIPRINRESSLYGSEFEIQLRNQLTQR
                     AIAKECADWIRSKVTFKSNKTQAPMQQFAHVQTAEDSTAYMPLQGFTAVDLGYQKGNA
                     VSNFIHRMDDKGPTQQYLNLFESIWQDQKQLEDVTALVTQHIESAYKENSPERIYFIL
                     LYNIFKDFLQDVDEDVLPNEQTGYQDTLVWNKLFNFQKDAATGIINKLETYNGCILAD
                     SVGLGKTFTALAVVKYYELRNKSVLVLVPKKLADNWLTYNRNLTTNIFAKDRFNYDVL
                     CHTDLSRTSGESFGTPLNQVNWGNYDLVVIDESHNFRNNDAIKDRETRYQTLMNKVIR
                     AGVKTKVLMLSATPVNNRFNDLRNQLALAYEGESSE"
     gene            1489..>1977
                     /locus_tag="VFC74_02395"
     CDS             1489..>1977
                     /locus_tag="VFC74_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24220.1"
                     /translation="MKNKIILISATVFFLSGCTAVKHQAMKSEADGTVLAVLDKFVKD
                     TQAGLFSPYNIYDISGINPGLTTWDKKYPALQKWWCVDYYTEESVKLKETIKSYCAKK
                     NGVYENSWCSVNSKPLFMVKTGTGKIVDRNSGCSYGSRSMGILAVTGEKIADQSSWVA
                     WAK"
BASE COUNT          575 a          380 c          453 g          569 t
ORIGIN      
        1 ctcgctcgac tcaccttcat aagctaaagc cagctggtta cgtaaatcgt taaagcggtt
       61 attcaccggc gtagcagaaa gcatcagcac cttggttttt acgcctgcac ggatcacctt
      121 gttcattaag gtttggtagc gggtttcacg atctttaatg gcgtcgttgt ttctaaagtt
      181 gtgggattca tcaataacga ctaaatcgta attgccccag ttcacttgat tgagtggcgt
      241 gccgaacgac tcgccactgg tacggctgag gtctgtatgg cagagcacat cgtagttaaa
      301 gcggtctttg gcaaaaatat tggtggtgag gttacggtta taagtgagcc agttatcagc
      361 cagctttttc ggaaccagca ccagtaccga cttgttacgc agctcgtaat atttaaccac
      421 cgccagcgca gtaaaggttt tacccaaacc cacgctgtcg gccaggatgc aaccgttata
      481 ggtttccaac ttattgatga ttccagtggc agcatctttc tggaagttga acagcttatt
      541 ccaaactaga gtgtcttgat aaccggtctg ctcgttgggc aatacgtctt cgtcaacatc
      601 ttgcaaaaag tctttgaaaa tattgtagag caggatgaag taaatacgct ccggcgagtt
      661 ttctttatag gcagactcaa tgtgctgagt gactagcgct gtgacatcct caagctgttt
      721 ttgatcctgc cagatgcttt caaacagatt gagatactgc tgggtagggc ctttatcgtc
      781 catgcggtga atgaagttag aaactgcatt acctttctga taacccaaat ctaccgcagt
      841 aaaaccttgc agcggcatat aggcggtgct gtcttctgcc gtttgcacat gagcaaactg
      901 ctgcatgggt gcttgggttt tattggactt aaaagtgacc ttgctgcgaa tccaatctgc
      961 acactctttg gcaatggcac gctgggttag ctgattgcgc agttgaatct caaactctga
     1021 accatacaag ctgctttcac gattaatacg cggaatgtag aactcacgct gctctttttt
     1081 gagcttatcg gtggcttgct ctggcacaaa ggtcggcgag gtaaagataa actcaagcga
     1141 ctcgacgcta ttgagctctc tttttaatgc agcataggca taaatagaaa aacaggaggc
     1201 ggcaattttc agtttggaat ttggggtcag tgcggttttt aagtcttccc caaaaagctg
     1261 gttcacattg tctaaaattt gcataagcgt cctgcttcaa agttcactgt caacaaagca
     1321 tacagtctac ccaatagtag agagtgatgc atcatgaaga aatgctgtgc taaaggctgg
     1381 ttgtataacg cataaatatt gctatattta tgcgtttcat cagtacttac gttatacgta
     1441 tagaaatata aaaattcata aagggttaaa ataaacacag agtgagatat gaagaataaa
     1501 attattttaa tttctgccac agtatttttt ttaagcggtt gtactgctgt aaagcatcaa
     1561 gcaatgaaaa gtgaagctga cggtactgta cttgcggtct tagataagtt tgttaaggat
     1621 acgcaagctg gattatttag cccatataat atttatgata ttagcggaat aaatccagga
     1681 ctaacaactt gggataaaaa atacccagca ttacaaaaat ggtggtgtgt agactattac
     1741 actgaagagt cagttaagct caaagaaacc attaagagtt actgtgctaa aaaaaacgga
     1801 gtatatgaaa acagctggtg cagtgttaat agcaagcctt tatttatggt taaaacaggt
     1861 acaggtaaaa tagttgatcg taatagcggc tgtagttatg gatcaagaag catggggata
     1921 ttagctgtta cgggtgagaa aattgcagat caatcatcat gggttgcatg ggctaaa
//
LOCUS       DASRMS010000054         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_822781, whole genome shotgun sequence.
ACCESSION   DASRMS010000054 DASRMS010000000
VERSION     DASRMS010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_822781"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..335
                     /locus_tag="VFC74_02400"
     CDS             <1..335
                     /locus_tag="VFC74_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24221.1"
                     /translation="GAHGGEKEKNPLDEIITTFNERWFQGWSATPEEQRVKLINISDS
                     IKQHPDFETKYAKNPDPHNKELAFEKIFKEIMLQRRKDELELYKLFASDGAFNLSMVQ
                     TMQRMVAL"
     gene            672..1193
                     /locus_tag="VFC74_02405"
     CDS             672..1193
                     /locus_tag="VFC74_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24222.1"
                     /translation="MNKKISMDLVHEPTTGLLAEVYRVLSSVQFNAASQRQILEQIQQ
                     DSTAESLGLPPIEVIQQRQDNYLLCGSFRHYQLLQAAGAKTLHALILPAEAPPNSHAV
                     RAFRQGISTILVYDSTTKTPHPQLRELKKYLNSHASPELLQLIKNDAFLTSALRISAR
                     DLRTQLQKKVLWN"
     gene            1237..>2861
                     /locus_tag="VFC74_02410"
     CDS             1237..>2861
                     /locus_tag="VFC74_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24223.1"
                     /translation="MTECKFSADNMNALIQHFAVDELLFTQLCDWIQAPKNLQQFIAV
                     AENLNALLSVSLQQELAAELGLDKLPIDVWRVLLQQLQPKLSNVPPELIDIRPNKQVR
                     PAGMIGSYPVYATLTAAEVDYPTGEAEKVRVIHLIGMSAAIAYQQMLTIGVIKELSSG
                     FEAGLREIRDLEKTAKRPAVPLPDLRDAVSLTEVQEEIEQWIEQHSITTRWGSGLRLL
                     IESVAEQRSRASRHYRMRMSTQTQRSGQHISMQELDGTDATNKQQPSFEPTMRVPDTR
                     SKKLLQQQGLHSAEMTTLATVIVTDPTAPRSVQSDERLIRHRNRRRVQASIVQSAQSL
                     PNRVEMPTPYEVQALLHCLANDFIKKNLPNLNSDQHDEIKVSILLRLYTGRKLDSLRK
                     LLVFQNKVDYEAAQRAFFAVLIDESCLAFPVKSTANPHSLGAVGGVLLANEPFYHKEI
                     QLQDVLDFRLPPVTTQAVIDLATRKLQCASSRKRVPLFEYADDHEQWIRELLKKINQT
                     YATRWTEHRLGLVMRMAVEQCTQDPALVQLITE"
BASE COUNT          832 a          648 c          655 g          726 t
ORIGIN      
        1 gtggtgctca tggcggtgag aaagaaaaaa atccgctcga cgagatcatt actacgttta
       61 acgagcgctg gttccaaggc tggagcgcta cacctgaaga gcaacgtgta aagctgatta
      121 atatttctga cagcatcaag caacatccag attttgaaac taaatatgcc aaaaacccag
      181 atccacacaa caaggagctg gcctttgaaa agatatttaa agaaatcatg ctgcagcgca
      241 gaaaggatga gctagaactg tataagctct ttgctagtga tggcgcgttt aacttgtcta
      301 tggtgcagac catgcagcgg atggtagcgt tgtaagaatg aaatacgctg gttaggttat
      361 aggaaaacgc tgaataatta tctgcgtggc caagacgttg ctaaacaatc agctctgact
      421 gcctcgctaa cattattcag tgtttccata agcctgagct agggttattt gtggtgttcg
      481 cagagtgcat catatttaag agagatcagc tttctttgaa aatcgagcgt cttttttacg
      541 ttttttcgaa aaaagaagct atgttacatt ttccgtcact tatcgacacc tgaccttcgc
      601 ccaccctgcc ataaaatccc tctaacgccg taaaaaacca gttcatggca ctttacattt
      661 agagggtcgg tatgaataaa aaaatatcaa tggatttagt gcatgagcca actacagggc
      721 tactagccga ggtctaccgg gttttatcat ctgtccaatt taatgccgcg tctcagcggc
      781 aaatactcga gcagattcag caggacagca ccgctgaatc gctgggtctt cctcctattg
      841 aggttattca acagcgtcaa gacaattatt tgctctgcgg tagctttcgc cattatcaat
      901 tgttgcaagc agcaggtgca aaaaccttac acgcccttat tttgcctgct gaggcgcccc
      961 ctaatagtca tgctgtgcga gcatttcgtc agggtatcag cactattctg gtgtatgaca
     1021 gcaccacaaa aaccccacac ccgcagctgc gcgaactgaa aaagtatctg aacagccatg
     1081 ctagcccaga gctactacag ttaattaaga acgatgcgtt tttaacaagc gcgttgagaa
     1141 tcagtgctcg ggatcttcga actcaactcc aaaaaaaagt actttggaat tgatggtcaa
     1201 acagttaaaa attaacgcct gagtattgga gcttgcatga cagagtgtaa attttctgca
     1261 gataacatga acgccttgat ccagcacttt gctgttgatg agctgctttt tacccagctt
     1321 tgcgactgga ttcaagcacc gaaaaaccta cagcaattta ttgcagttgc agagaatctg
     1381 aatgcattat taagtgtcag cctgcaacaa gaattggctg cagagcttgg gttggacaag
     1441 ctgcctatag atgtatggcg agtactactg caacagctac agccaaaatt atctaatgtg
     1501 cctcctgagc tcatagatat caggccaaac aaacaagttc ggcctgcagg gatgattggc
     1561 agttatcctg tttatgcaac gctgacggct gctgaggtcg attacccaac aggagaagct
     1621 gaaaaagttc gagtgattca cctgattggc atgagcgctg caattgccta ccaacaaatg
     1681 cttactatcg gcgttattaa agaattaagc agtggttttg aggccggttt gcgtgaaata
     1741 cgcgacctag aaaaaacagc taaacgacca gcagttccac tacctgactt acgtgatgct
     1801 gtttcactga ctgaggttca ggaggaaata gaacaatgga ttgagcagca ttctattact
     1861 acccgatggg gttcaggtct acggctacta atagagtctg ttgctgagca gcggtcgcga
     1921 gcatcacgcc attaccgtat gcgtatgagt actcaaaccc agcgcagtgg tcagcacatt
     1981 agtatgcagg aactagatgg cacagatgct actaacaagc agcagcccag tttcgagccc
     2041 actatgcgtg tgccagatac tcgcagtaaa aaactgctgc aacagcaggg tttacactct
     2101 gctgagatga caacactggc aaccgttatc gtcacagatc caactgcacc tcgcagtgtt
     2161 caatcggatg agcgattaat acggcaccgc aatcgtcgac gggtacaggc atcgattgtg
     2221 cagtcagcac aatccctacc taatagagtg gaaatgccaa ccccgtatga agttcaagcg
     2281 ttactccatt gtttggccaa tgattttatt aagaaaaatc taccaaacct taatagcgac
     2341 cagcatgacg aaattaaggt gagcatccta ttgagactgt atacgggtcg taaattagat
     2401 tcgctgcgta aattactcgt tttccaaaat aaagttgatt acgaggctgc tcaacgagca
     2461 ttttttgccg tactcataga tgaaagctgt ttagcatttc cagtaaaatc aacagcgaac
     2521 ccccactcat tgggcgcagt tggtggtgtt ttactggcaa atgagccgtt ttaccataag
     2581 gagattcagc tgcaggacgt actggatttc agactgccgc cagttactac tcaggcagta
     2641 attgacctag cgacacgcaa gctgcagtgc gctagcagcc gaaaacgagt tccgttgttt
     2701 gagtacgcag acgaccacga acaatggatt cgtgagcttc tgaaaaaaat caaccaaact
     2761 tacgcaactc ggtggacaga gcatcgtcta ggtttggtta tgcgcatggc agttgagcag
     2821 tgcacgcagg atcctgcact ggttcagctt attactgagc a
//
LOCUS       DASRMS010000055         3708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_952506, whole genome shotgun sequence.
ACCESSION   DASRMS010000055 DASRMS010000000
VERSION     DASRMS010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952506"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..488)
                     /locus_tag="VFC74_02415"
     CDS             complement(219..488)
                     /locus_tag="VFC74_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZK24224.1"
                     /translation="MMIAAVITLGGLGFIFGCILAYAAQKFAVEVDPRIEKIQACLVG
                     ANCGACGYGSCTQFAEAVVAGKAPSTGCKPGRAKVAAKIAEIMGE"
     gene            complement(507..1082)
                     /gene="rsxA"
                     /locus_tag="VFC74_02420"
     CDS             complement(507..1082)
                     /gene="rsxA"
                     /locus_tag="VFC74_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HZK24225.1"
                     /translation="MTSILTIVFGAIFINNFVLSRFLGICPFLGVSKKVETSLGMGMA
                     VTFVMGMASLVTWLLNRYVLIEYSLEYLRTIVFILVIAALVQFVELFIRKVSPVLYQA
                     LGIFLPLITTNCAVLGVALLNIQLEYNLLESVVFGVAAALGFTLALVIMAGIRERLEM
                     AFIPKALRGVPIALITAGILSLAFMGFQGMM"
     gene            complement(1100..1699)
                     /locus_tag="VFC74_02425"
     CDS             complement(1100..1699)
                     /locus_tag="VFC74_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HZK24226.1"
                     /translation="MSVMQELTKGLFKENPTFRLVLGMCPTLAVTTLAVNGLGMGLST
                     AAVLVASNLVISLLRNLIPSKIRIPAYIIVIATFVTVVDMLLNAFQPDLHSQLGIFIP
                     LIVVNCIILGRAEAFASKHGVVASMADGLGMGLGFTLSLTVLGSLRELVGNGTLFGAN
                     ILGAAYEPFKVFASPPGAFIALGLLMALMNLLVKKYKLE"
     gene            complement(1715..2803)
                     /locus_tag="VFC74_02430"
     CDS             complement(1715..2803)
                     /locus_tag="VFC74_02430"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZK24227.1"
                     /translation="MKDIVRSGLILMLICALSAGMLSYTHGVTSEIIEARIAAEKIAL
                     MQELFPAVATTEDQEVDGRSATVGYDESGKVVGILAEGKATGYRGDIRFNLAIDGTGK
                     LVNLNIISHSETASLGSRIEEEAYRAQYEGKTVNDSFDVDNISGATVTSVAMERGVAS
                     EMKEILLRFGEGLVEPVPSIDITQLADGTYEGSGEGLKSTITVAVTVAGGKITAVEIV
                     SGNDTEEYYTKAKKEVPARIVEEQKVDVDAASGATISSEGIKDAVLDALQKPPVLKLS
                     EIADGTYEGSGEGLKSTITVEVTVAGGKITAVEVVSGNDTEEYYAKAKKEVPARIVEE
                     QKVDVDAASGATISSEGIKDAVRNALQQ"
     gene            complement(2803..>3708)
                     /locus_tag="VFC74_02435"
     CDS             complement(2803..>3708)
                     /locus_tag="VFC74_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZK24228.1"
                     /translation="MDKKFVVSSSPHLRTDETIKSVMVDVLVALTPAVIAAVIFFGYR
                     ALITMALTTLTAMLTEAIILRNKNFFGDGSAAVTGLLLAMTLPPAPPWWLCVAGAVVA
                     IIIGKQVYGGIGNNIFNPALVARAVLMVSWAGHMTTWLSPVDLTSTATPLVVSTPPGF
                     LQLFTGATGGCLGETSAFALLFGAVWLFYKGHINWRIPGGYLGVVFLMGMALDGGLTG
                     GVFHLLAGGVMLGALFMATDMVTSPVTTRGQLVFGIGCGLITMLIRFYGNYPEGVTYA
                     ILLMNALTPLIDNLTLPRKYGEVAK"
BASE COUNT         1060 a          942 c          765 g          941 t
ORIGIN      
        1 atatatacag gtattaacta tagtgtacac tgtgtcctca aaaactgtca acacgtacat
       61 aattgtctta aatgccattt agcacctaaa tcaagaaaaa cgcttcgcgt ttttttcttt
      121 tgaggcgtct ttcttgtact ggcaaggaat atataccaag taaaaacggt gcagaggatt
      181 taccaatgca ccgttacgct aaaagaatct tctatagttt attcacccat gatctctgca
      241 attttagcag caaccttagc tcgacctggc ttacaacccg ttgatggcgc ttttcctgcc
      301 accactgcct cagcaaactg agtacagctg ccatagccac aggcgccaca gttggcacca
      361 actaaacatg cttgtatttt ttcgattctc gggtcaactt caactgcaaa tttttgtgca
      421 gcataggcca aaatacaccc aaagataaag ccgaggccgc ccaaagttat tactgctgct
      481 atcatcattt tattttgtta tgcctcctac atcatacctt gaaagcccat aaaagcaagg
      541 gatagaatgc cagctgtgat cagagcgatg ggtactccgc gtaaggcttt aggaataaaa
      601 gccatctcta aacgctctcg gataccggcc ataattacca gcgccaaagt aaagccgagg
      661 gcagcagcca caccaaagac tacggactcc aacagattat actctaactg aatatttaac
      721 aaggccaccc ctagtacagc acagttggta gtgatgagcg gcaaaaaaat ccccagcgct
      781 tggtaaagta cgggacttac tttacggata aagagttcca caaattgtac cagcgctgca
      841 attaccagga taaaaacaat ggtgcgcaga tattccagac tatactctat taacacatag
      901 cggtttaata accaggtcac cagtgaggcc atacccataa caaaggttac ggccataccc
      961 atacccagag atgtttctac tttcttggaa acgccaagga aagggcagat cccgagaaat
     1021 ctactcagta caaagttatt gatgaaaata gcaccaaaga caattgtcaa tatactcgtc
     1081 attatctact ccctccttgt tattccaatt tatatttctt gaccaacagg ttcatcagag
     1141 ccattaacaa gcccaaagca atgaaggctc ctggcggtga ggcaaatact ttaaagggtt
     1201 cataggcggc acctaagata ttggcaccaa aaagtgtgcc gttgcccacc agttctcgta
     1261 aagagcccaa taccgttaaa gaaagggtaa aacctaagcc catacctagt ccgtctgcca
     1321 tggaggcaac tactccatgt ttagaggcaa aggcttcagc tctgcctaaa ataatgcagt
     1381 tgaccacaat cagcgggatg aagattccca gttgggagtg caaatccggt tgaaaagcat
     1441 ttaagagcat atcaactacc gtcacaaagg tggcaataac gatgatataa gccggaatgc
     1501 gtattttgct gggaataaga ttgcgcagca gggaaattac caagtttgaa gctaccaaga
     1561 cagcagcggt ggaaagaccc atccctaagc cattaacggc cagtgtggtt accgccagtg
     1621 tcgggcacat ccccagcact aatcggaagg tcgggttttc tttaaataaa cctttagtta
     1681 gttcttgcat tacactcatt atcctcaccc cccgttattg ctgcagtgca ttgcgcactg
     1741 catccttaat gccttcgctg cttatggtag caccgctggc tgcatccaca tcaacttttt
     1801 gttcctccac aatacgtgct ggcacttctt tttttgcttt ggcataatat tcctcggtgt
     1861 cattaccgga gactacttct accgcggtga tcttaccacc tgccaccgtt acttctaccg
     1921 taatggtaga tttcaagcct tcgccgctgc cttcataggt gccgtcggca atctctgaga
     1981 gtttcaaaac aggtggtttt tgcagcgcat caagaaccgc atctttaatg ccttcgctgc
     2041 ttatggtagc accactagct gcatccacat caactttttg ttcctccaca atacgcgctg
     2101 gcacttcttt tttcgctttg gtataatatt cctctgtgtc attaccggag actatttcca
     2161 cagcggtgat tttaccacct gccaccgtta ctgccacagt aatggtggat ttcaaaccct
     2221 caccgctgcc ttcgtaggtg ccatcagcta actgcgtaat atctatagat ggtaccggtt
     2281 caaccagacc ttcaccaaag cgcagcagaa tttctttcat ttcactggcc acaccacgtt
     2341 ccatggcgac actggtaacg gtggcaccgg aaatattatc gacatcaaag gagtcgttaa
     2401 cagttttgcc ttcatattgc gcccggtacg cttcttcttc aattctagag ccaaggctgg
     2461 ccgtttcaga gtgcgagata atatttaagt taactagttt acctgtaccg tcaatggcta
     2521 aattaaagcg aatatcgccg cgatagccag tggctttgcc ttcggctaaa atccccacca
     2581 ctttgccgga ctcatcatag cccacagtgg cgctgcggcc atcaacttcc tgatcttccg
     2641 ttgttgctac tgcaggaaat agctcctgca ttaaagcaat tttctctgct gcaatccggg
     2701 cttctatgat ctccgaggtt acgccatgag tataggagag cataccggcc gaaagggcac
     2761 agattaacat taaaattaaa cctgaacgta caatatcttt cattatttag ccacctcccc
     2821 gtactttctg ggtagtgtca gattatcaat taacggggtc agggcattca ttaacaaaat
     2881 ggcgtaggta acaccttcag gataattacc atagaaacga attagcatgg taattaagcc
     2941 gcagccgata ccaaagacaa gctgtccccg cgtggttacc ggggaagtta ccatatcagt
     3001 agccataaaa agtgctccta acataacgcc ccccgctaaa agatggaaga cgccgcctgt
     3061 cagaccgcca tctaatgcca ttcccattaa gaagacaacc cctagataac cgccgggaat
     3121 acgccagtta atatgtcctt tgtaaaagag ccacactgca ccaaagagca gggcaaaagc
     3181 acttgtttcg cctaaacagc cccccgtggc accggtaaac agctgcagaa aacccggagg
     3241 ggtagaaact actaaaggag tggctgtgga agttaaatca acggggctga gccaggtggt
     3301 catatgaccg gcccaagaaa ccattaagac tgcccgtgcc accagtgccg ggttgaaaat
     3361 gttgttgcca ataccaccat aaacttgctt gccgatgata atggcgacca ctgcaccggc
     3421 tacacatagc caccacggtg gtgctggggg caatgtcatg gctaacaata aaccagtgac
     3481 tgcagcactg ccatcgccaa agaaattttt attacgtaag ataattgctt ccgtcagcat
     3541 ggcagtgagc gttgtgagcg ccatggtaat taaagcgcga tagccaaaaa agatcacagc
     3601 ggcaatgact gccggtgtta atgctaccag tacatctacc atcacactct ttatggtttc
     3661 gtctgttctt aagtgtggtg aagatgaaac gacaaatttc ttatccat
//
LOCUS       DASRMS010000056         1009 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_885688, whole genome shotgun sequence.
ACCESSION   DASRMS010000056 DASRMS010000000
VERSION     DASRMS010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1009)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1009
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_885688"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            511..>1009
                     /locus_tag="VFC74_02440"
     CDS             511..>1009
                     /locus_tag="VFC74_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK24229.1"
                     /translation="MKTCLNGHSVPDDAKFCAVCGIPVEDTQPEVMELVKTCPNGHEV
                     QDEAKFCLECGAAIVQSAPSEQPAAASSQYAQPQQAEPSQQPAGVPSQYVQPQQAQAQ
                     NYPGYQQYPPGQQYPANQGYQQYQYQQHPQPAVAQKGISTIFPSVVMMIASVMLIVSV
                     FLPYIV"
BASE COUNT          278 a          226 c          240 g          265 t
ORIGIN      
        1 catctggcgc tggaatacgc tggctgccct cttattggtc gtactaggta tttccctgat
       61 taattggaag cgaaagactg ttgccccggc ggtggggaat ccgcggactt agaagttgcc
      121 ggtaccgcgg caagcggcaa ggcggtacag ttgatggtgc caggtgttga cttgttgact
      181 tattgcgacg gtccggttga tgaacttgaa aacacaaata aaaacacaaa ttctgcttgt
      241 acataaattt tttttgcgaa ggctctgata gcgtaataat atttgttata ctgcaaacag
      301 catacaatga atttgtctga gtatgcctgc tgtcaaagca gctaatatga caggttctaa
      361 aaatgcattt gatattaatt aagactttgg tgcttggata tgtcctagtg tcactgcccg
      421 gtggtactgt cagagaccag gatttgctcc ggcagtacta cccaagtatt ggattagtcc
      481 gagagccatt gccaagaaga ggagagaatt atgaagactt gtcttaatgg ccatagcgtc
      541 cccgacgatg caaaattctg tgcagtatgc ggaattccgg tggaggatac gcagcctgaa
      601 gtgatggagt tagtaaagac ctgccccaat ggtcatgaag tccaagatga ggcaaagttt
      661 tgcctcgagt gtggcgctgc cattgttcaa tcagcaccat ctgagcaacc ggctgcagca
      721 tctagtcaat atgctcagcc acagcaagcc gaaccaagcc aacagccagc aggagtacct
      781 agtcaatatg ttcagcccca gcaagcacag gcgcaaaact acccaggata tcaacaatat
      841 cctcccggac agcaataccc tgccaaccaa ggatatcagc agtatcagta tcagcaacac
      901 ccgcagcctg ctgtcgctca aaaaggcata agtactattt tcccatctgt tgttatgatg
      961 attgcttcag ttatgttaat agtgtctgtt tttttaccat atattgttt
//
LOCUS       DASRMS010000057         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_751569, whole genome shotgun sequence.
ACCESSION   DASRMS010000057 DASRMS010000000
VERSION     DASRMS010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751569"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..403)
                     /locus_tag="VFC74_02445"
     CDS             complement(44..403)
                     /locus_tag="VFC74_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24230.1"
                     /translation="MFTGGLIDIVTYYAGVYGWQMNAMANGYAWIILSAALILWTPAY
                     LKEAPKVFSSLVVLLDVFVVCVTLIKLQMATPAVITIASAFAFVAIFVAMYFATAMIT
                     NNAFGKVVLPVGTPWIK"
     gene            623..2119
                     /gene="istA"
                     /locus_tag="VFC74_02450"
     CDS             623..2119
                     /gene="istA"
                     /locus_tag="VFC74_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HZK24231.1"
                     /translation="MIQQYHIKHLNFHKGKSLRRIAEETGHDFRTVKKYAEQTDFNEI
                     RKPRRGRPSKLDPVKPIIDAWLKEDLNRPVKQRHTARRIYDRLCEEHQDIFNAGERTV
                     RSYVSARKKELYGEEEGYLPLEHPAGEAQVDFGEIIMIEQGQKVKGYELILSLPYSNA
                     GYPQIFRGQNQECLLTGLRDIFEYLKHVPRIIWFDNMSAAVTGIGEKGERKLVDQFYR
                     FALHYGFKPQFCNPGKGHEKGHVENKVGYSRRNYFVPEPSFSDIEEFNQGLFAVAEKD
                     HCRKHYRKGRVINELLQDDLTAMLPIPAKPFAIGRIQKLSANKYGKVCVDTNIYSASP
                     QVANKELYMRLGAHQVELLNEQYHPIVKHKRLYGRNQELMDWLPYLITLVKRPNALKY
                     TGFYRELPDPWQDYFNNLDYAEKKKSLNLLVRMISETDMDTATICLLETLDFGKADAD
                     SILLSYRRLTEPTFNDFLPLLSAGINSPAIYTPDLTCYDTFLKAGECR"
     gene            2116..2838
                     /gene="istB"
                     /locus_tag="VFC74_02455"
     CDS             2116..2838
                     /gene="istB"
                     /locus_tag="VFC74_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZK24232.1"
                     /translation="MKELIKDYCKQLRWGNSIVQNYADIKADTHEEFLAKLLETELKV
                     RELNRKNRCLRQAGFDVIKTFNGYSFDHIEIPASIPLDDLKTAALVKNRENLILYGPV
                     GTGKTHMATAIGVEACNQGKKVGFYRTSTLVNELNDAQNAGNLGKMLKQLSKLDLLIC
                     DEWGYIPLDRQGAQLLFQVVADCYEKRSIIITTNLEFSKWNGIFYDEKLTSAIIDRLI
                     HHSHLLVFGGQSYRLIHSTIRK"
     gene            complement(3345..3422)
                     /locus_tag="VFC74_02460"
     tRNA            complement(3345..3422)
                     /locus_tag="VFC74_02460"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3385..3387),aa:Pro,seq:cgg)
BASE COUNT         1118 a          757 c          746 g          875 t
ORIGIN      
        1 catatccatt attatatttg aagagcccaa aataaaaccg ttattactta atccagggtg
       61 tccccacagg taataccact ttgccgaatg cattgttggt tatcatagca gtagcaaaat
      121 acatagccac aaaaatcgcc acaaacgcaa aagctgatgc aatggtaatg accgccggtg
      181 ttgccatctg tagtttgatt aaggtaacac aaacgacaaa tacgtccaag agaacaacca
      241 aagatgaaaa caccttagga gcctctttaa gatatgctgg agtccatagt ataagagcgg
      301 ctgacagtat aatccatgcg tacccgtttg ccattgcatt catctgccac ccgtagacac
      361 cggcataata tgttactata tcaatcaggc caccagtaaa catgaagaac cccgcaaaga
      421 ataagaacac attgccaccg gttagtgctc cttcctttaa atcaataatt gcacaggttg
      481 tttatggcac aataaaaata cagcgaatgg caagctaaaa atacagcagt aaattggaaa
      541 aaaatcatcg ccggcacccc caaaatatac tacccttttt gttgaagaga caaaaggagg
      601 cagataagaa aggatgctaa cgatgataca acaatatcat atcaaacact taaactttca
      661 taaaggcaaa tctcttagga ggattgccga agagaccggg catgatttca ggactgtaaa
      721 aaaatatgca gagcaaaccg atttcaatga aatccgaaaa cctagaagag gacgaccgtc
      781 gaagcttgac ccggtaaaac cgataattga cgcctggctt aaagaagatc tgaaccggcc
      841 agttaaacaa cggcacactg ctcggcggat ctatgaccgc ctgtgtgaag aacatcagga
      901 tatatttaat gctggtgaaa gaactgtccg ctcttatgta tctgccagaa aaaaagaact
      961 atacggtgaa gaagaaggct accttcccct ggaacaccca gcaggagaag cacaggtaga
     1021 tttcggcgaa atcatcatga ttgagcaagg ccagaaagtc aaaggatatg aattaattct
     1081 ttcattgccg tacagtaatg ctgggtatcc ccagatattt cggggtcaga accaggaatg
     1141 cctgttaacc ggactaagag acatatttga atatttaaag catgttcccc ggataatctg
     1201 gtttgataat atgtcagcgg cagttactgg tattggggaa aaaggagagc ggaaactagt
     1261 ggaccaattt taccgcttcg ccctccacta cggctttaag ccccaatttt gcaaccccgg
     1321 caaagggcac gagaagggcc atgttgaaaa caaggtgggt tacagccgca ggaactactt
     1381 tgtacccgaa cccagtttta gtgatattga agaattcaac cagggacttt ttgccgtggc
     1441 tgaaaaagac cactgccgga aacattaccg taaaggccgg gtaataaatg agcttctcca
     1501 ggatgacctt acagccatgt tgcccatacc tgccaaacca tttgcaatcg gccgtataca
     1561 gaaattatcg gccaacaaat acggcaaagt ctgtgttgat acgaacatat attccgcatc
     1621 accccaggtg gcaaataaag aactatacat gaggctaggt gcccatcagg tggaactatt
     1681 aaacgaacaa taccatccta tcgtaaaaca taaacgatta tatggccgga atcaggaact
     1741 aatggattgg ctgccttacc taattacact ggtcaaacgc cctaatgctt tgaaatatac
     1801 tggattttat cgtgaactac ctgacccctg gcaggactat tttaataatc tggattacgc
     1861 ggaaaaaaag aagagcctaa atcttttagt acgtatgata agcgaaacag atatggatac
     1921 agcaaccata tgcttgctgg agacgttaga tttcggcaaa gcagatgctg acagtatcct
     1981 gttaagctac cgccgattaa ccgaaccaac ctttaatgat tttttgccgt tattatctgc
     2041 cggcataaat tcaccggcaa tatatactcc tgatctgacc tgctatgata ctttcctgaa
     2101 agcaggtgag tgcagatgaa agaattaatt aaagactatt gcaagcaatt acgctggggg
     2161 aacagcatag tacagaacta tgcagatatt aaggctgata cccacgaaga atttctagct
     2221 aaacttttag aaacagagct taaggtacgg gaattaaacc gtaaaaaccg ctgtttaagg
     2281 caagccggtt tcgatgtaat aaaaaccttt aatggctata gtttcgatca tattgagata
     2341 ccggcatcaa taccattaga tgatttgaaa actgcagccc ttgtaaaaaa cagggaaaac
     2401 ctgattctat atggcccagt gggaacgggc aaaactcata tggctaccgc catcggtgta
     2461 gaagcctgta atcaaggaaa gaaggtggga ttttatagaa catcaaccct ggtcaacgag
     2521 ttaaatgatg ctcaaaatgc tggtaatctg ggcaaaatgc taaagcaact atcgaagctg
     2581 gatttactaa tttgcgatga atggggttat attcccctgg atcgccaggg tgcacagcta
     2641 ctctttcagg tagtagctga ttgctatgaa aaacgcagta ttattattac taccaactta
     2701 gaatttagta aatggaatgg catattctac gatgagaagt taaccagtgc gattattgac
     2761 cgcttaattc accatagtca tctactggtt tttggtggac aaagctacag acttatacat
     2821 tccacaatca ggaaataatg gaaactgggg tgctggattt attttttgcc gtttgatgta
     2881 ttttatactt gccaaacaca gacggtaggc ttaggcctac cgtttattca tattgcttac
     2941 aatacgcaag cacacaaagc cgtccccttc ccccaaaacc acttgttttt ccctatttcc
     3001 ttatggaatt acaccactgg ccctcttagt agacataaaa aaatcccgcc atacagcagg
     3061 aaccttggga tttgttttgg ttggccttgt gacagaaggc ataaaaaccc ttaatgtcaa
     3121 tgtctcgatt ctcagggatt gctaggacct gctggggcct gctagggcct gctagggcct
     3181 gctagggcct actagggcct gctagggcct actagggctt gctagggcct gctggggctt
     3241 gctagggcct gctggggctt gccgggaaac tatagcaagc ttcaatctgg tttttcgaca
     3301 tgtccctaag taccgataag cttgggtttc aaggtgctaa aacatggtcg ggatgacagg
     3361 acttgaacct gcggcctcct ggtcccgaac caggcgctca taccaaactg agctacatcc
     3421 cgacgcaaca cctattttac cttaagcata gataaatttc aatattaatc gtatggttaa
     3481 taacaaaatg ctatac
//
LOCUS       DASRMS010000058         1726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_550330, whole genome shotgun sequence.
ACCESSION   DASRMS010000058 DASRMS010000000
VERSION     DASRMS010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_550330"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="VFC74_02465"
     CDS             complement(<1..296)
                     /locus_tag="VFC74_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen flippase"
                     /protein_id="HZK24233.1"
                     /translation="MTLIKTSLLNGIAVVIKMLTMLGLNKILAIYVGPAGYAAIGNFQ
                     NAAQMITTFASGAINTGVVKYTAEYHDDEEKQRQVWRTAGTIAVLGSVFTGIGVA"
     gene            complement(321..1439)
                     /locus_tag="VFC74_02470"
     CDS             complement(321..1439)
                     /locus_tag="VFC74_02470"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009460470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HZK24234.1"
                     /translation="MIPFLDLKAINAQYREELIEACTRVIESGWYIAGNELAQFEQAF
                     ASYCGTKHAIGVANGLDALILTLRAWKELGKLKDGDEVIVPSNTYIASILAISANDLV
                     PVLVEPDVATYNLCPKQTEAAITAKTRAILPVHLYGQLADMPAIMAIAQRHNLLVLED
                     SAQAHGAELNGKKAGNWGDASGFSFYPGKNLGALGDAGAVTTNDDQLAETLRALRNYG
                     SHEKYKNLFQGTNSRLDEIQAAMLSVKLKHLDTEIAHRRKVADAYIQGINNTAVTLPL
                     TLNSSPLTRENHVWHVFVIRCEQREALQKHLLEHGIQTLIHYPIPPHQQQAYKEWNMD
                     SYPVSELIHQQVMSLPMGPTVTDEQVAQVIAAINTFKI"
     gene            complement(1436..>1726)
                     /locus_tag="VFC74_02475"
     CDS             complement(1436..>1726)
                     /locus_tag="VFC74_02475"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZK24235.1"
                     /translation="VYDLKKDSFCWGSWILNEDKTRYAALESAFLVYKFGFEVLGFKK
                     SHFDVMKGNERVISFHKKMGAVQTGEDEQNYYFEITNSAVYAMQEKLASKIQ"
BASE COUNT          404 a          385 c          413 g          524 t
ORIGIN      
        1 gctacaccta ttccggtaaa gactgagccc agcacagcaa ttgtgcccgc agtgcgccac
       61 acttgccgtt gcttttcttc atcatcatgg tattcagcgg tgtatttcac tacaccggta
      121 ttaatcgcac cgctggcaaa ggtagtgatc atttgtgcag cgttttgaaa gttaccaatc
      181 gccgcatagc ctgccggccc aacgtagata gcaagtatct tgttgagccc aagcatggtg
      241 agcattttta tcactaccgc aatgccgtta agcaggctag tttttatcag tgtcacagga
      301 ttaattctta atgagtaatt ttaaattttg aatgtgttta tggctgcaat cacttgcgca
      361 acttgctcat cggtaacagt cggccccatc ggcaagctca tgacttgctg gtgaatcagc
      421 tccgacaccg gataactatc catattccac tctttataag cctgctgctg atgcggtggg
      481 attgggtagt ggattaaggt ttgaatgcca tgttctaaca agtgcttttg caaggcttcg
      541 cgttgctcac agcggatgac gaacacatgc cagacgtgat tctcacgagt taggggtgaa
      601 gagttaagtg tgaggggtaa agttacggct gtgttgttga taccttgtat gtatgcgtca
      661 gctacttttc ttctgtgtgc tatttctgta tctaggtgtt ttagcttgac gctgagcatc
      721 gctgcttgta tttcgtcaag gcggctgttg gtgccttgga ataggttttt atatttttcg
      781 tgtgagccgt agttgcgcag tgcgcgtaaa gtttcggcga gttggtcatc attggtcgtg
      841 actgcaccgg catcgcctag tgcaccgagg tttttacctg ggtaaaagct aaaaccgctg
      901 gcatcgcccc agttacccgc ttttttgccg tttagttctg ctccatgggc ttgtgcagaa
      961 tcttctagta caagtaagtt atggcgctgg gcgatagcca taatggcggg catatcagct
     1021 aactggccgt ataaatgcac agggagaatg gcgcgtgttt tagcggtaat cgccgcttcg
     1081 gtttgtttcg ggcatagatt gtaagtggct acatctggct caacgagcac cggcacaagg
     1141 tcattggcgc tgatggctaa aatgctggca atgtaggtgt tggacggcac aatcacttcg
     1201 tcgccgtctt taagcttgcc caactctttc cacgcgcgca gggtgagaat cagtgcatca
     1261 aggccattag ccacaccaat cgcgtgctta gtgccgcagt agctggcgaa cgcctgttca
     1321 aattgtgcaa gttcattacc agcgatgtac cagccagatt cgataacacg ggtacaggct
     1381 tctataagct cttcgcggta ttgggcgttg atggctttga ggtcgaggaa tgggatcatt
     1441 gtattttact cgctagtttt tcttgcattg cataaacggc actatttgta atttcaaaat
     1501 aatagttttg ctcatcttca cccgtttgta ctgcacccat ttttttatgg aacgaaatta
     1561 ctctttcatt acctttcatg acgtcaaaat gagacttttt aaatccaagc acttcaaaac
     1621 caaatttata aactaaaaaa gcactttcta atgctgcata gcgagtttta tcttcgttaa
     1681 gaatccagct gccccagcaa aatgagtctt tttttaaatc gtatac
//
LOCUS       DASRMS010000059         1396 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_282155, whole genome shotgun sequence.
ACCESSION   DASRMS010000059 DASRMS010000000
VERSION     DASRMS010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_282155"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VFC74_02480"
     CDS             complement(<1..265)
                     /locus_tag="VFC74_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK24236.1"
                     /translation="METPLAKKFEESPELMLAWLLRDINQMTLSQLSQNLACHGLYVG
                     QPRILRLIHDYPGSTQRQIADRLFVSGASLSTSIKRLQKAKLVE"
     gene            629..>1396
                     /locus_tag="VFC74_02485"
     CDS             629..>1396
                     /locus_tag="VFC74_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HZK24237.1"
                     /translation="MKNLGFVILTILVIMVVVILLSFINHRIRLAKEADLFIAPGQIV
                     KVNGHDMHVYAEGDADAAATLIFMSGAGTSSPYLDFKSLYSLLSDKYRVAVVEKIGYG
                     FSEVADIDRDVDSMLSDTRQALALAGIEGPYVLCPHSMSGIEALYWAQKYPDEVETLI
                     GLDITVPASYENTKLTTAMTKLAKFATDTGLIRWIPSLAERDAAEQGNLTEAEKELSR
                     IIFYRRTATQTMINEAEQIKDNAASLPDQGIIDQPCLL"
BASE COUNT          404 a          294 c          303 g          395 t
ORIGIN      
        1 gctccaccaa cttggctttt tgcagtcttt ttatggatgt ggaaagagaa gctcccgaga
       61 cgaataaccg atcagcgatt tgcctttgtg tacttccggg ataatcatga ataagccgca
      121 atattcgcgg ctgaccgaca taaaggccgt ggcaagccag gttctgtgaa agctggctta
      181 gcgtcatttg gttaatatcg cgtaaaagcc aggctaacat cagctcaggt gactcttcaa
      241 atttctttgc taacggagtt tccaagaaac tgcctttcta tggtaattgt aacggcataa
      301 ttaattaact acttatttgt tagttagcta acaataaaga attatactcg acccgtgagg
      361 tcgaggcaag caaaaaaaag tcaattctcc aatccggtgc ataatttggt gccaggcacg
      421 attctatgca cgaccttagg cggcctgatt acggaaaacc tgtactcgtc catccggaaa
      481 gtttcttttt ttaattgctt tgtggtataa acttaaaaaa gaaattaact gctaaaactg
      541 gctttactaa attaacagcg gctgctatta agtttcagta ataaatactt aattagcttg
      601 tcaaagcagg agaggaaagc gtattatttt gaaaaatttg ggttttgtta ttctgactat
      661 cttggtaatt atggtggtag ttattctgct tagctttatc aatcacagaa tcagactggc
      721 taaggaagca gatttattta tagctccggg ccagattgta aaggtcaacg gtcatgacat
      781 gcatgtgtat gcagaaggag atgccgacgc cgcagctact ttgatcttca tgtccggtgc
      841 cgggacctct tcaccttacc tggactttaa atcactttac tctcttctga gtgataaata
      901 tcgcgttgct gttgtagaaa aaatcggcta tggctttagt gaggtagcgg atattgatcg
      961 tgacgtagat tcaatgcttt ccgataccag acaggccttg gctctggccg gcatcgaagg
     1021 tccctacgtg ctttgccctc actctatgtc aggcatagaa gctctttatt gggctcaaaa
     1081 atatccagat gaagtggaaa ctcttattgg tttagacatt acagtacccg cgagctacga
     1141 aaacaccaag ttaacgactg ccatgactaa attagctaaa tttgcaactg atacgggcct
     1201 gatccgctgg attccttctt tggccgaaag agatgccgcc gaacaaggca atctaaccga
     1261 ggcagaaaaa gaattatcaa gaattatttt ttaccggcgc acagctacac aaacgatgat
     1321 taacgaagct gaacagataa aagacaacgc agcttccctg cccgatcaag gaattataga
     1381 ccagccctgc ctgctg
//
LOCUS       DASRMS010000060         2056 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_825109, whole genome shotgun sequence.
ACCESSION   DASRMS010000060 DASRMS010000000
VERSION     DASRMS010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_825109"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1635
                     /locus_tag="VFC74_02490"
     CDS             <1..1635
                     /locus_tag="VFC74_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HZK24238.1"
                     /translation="ALAIVNTSEADFNKLSGAINNSAGAADRMAKEMQDNLKGRLVEL
                     KSAIEGVALQLYEAMQPALEKVVAAVQKLVDWLAKLSPGMQTAIIAIAGLAAAIGPIL
                     IVLGLLAQGVGAVIGVVGSLTAALPAFGAVLAALTGPIGLVVAAIAGLTAVWLIWGDD
                     IKRITSDVVSAVKEFFTDMGKKIAGTWDAVRAKTTETWNAIKSFLNTLWSNLKSTVEI
                     VFSAIKYSIESITRAIQSILSNIWNAIRNFLSETLDAIKVKFNEIWNTIKVFLEELWT
                     ALKDFAIAIFTSLQQNIETITLAIQALITDIWNAIKDFLDETWNTISSTAESVFNAIK
                     ELLNTIMNTIKSNTEEAWNAIRSFLSETLDAIKVKFDEIWSGLNQSTTDAWEAMKSTV
                     KASINGIIGFINDMIRAWNGISFTVPRVSVPFVGTFGGFSVGTIQIPEIPFLAKGGVV
                     TKEMLTVLGEAGPEAVIPLPKLQPMLSAALTAALGKPGGGATIDYDKLAAAVAKHVKP
                     SITQHNVFNSPEPLTPAETARQNLRVSRTLAMEWGL"
     gene            1637..>2056
                     /locus_tag="VFC74_02495"
     CDS             1637..>2056
                     /locus_tag="VFC74_02495"
                     /inference="COORDINATES: protein motif:HMM:NF017520.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail domain-containing protein"
                     /protein_id="HZK24239.1"
                     /translation="MSRKFVFTNSKGQSVEFTNDAPLFMKTYHGTGGSPVDIHTQKSP
                     HQDGEALINTTLEMRDIQLVVAILGNSTSEVMQHRRRLSQVLNPKLGRGKLRFESETG
                     AHEIDVQPNGAPAFPGGEARGALYQECLIELLAPNPFW"
BASE COUNT          601 a          426 c          534 g          495 t
ORIGIN      
        1 gcgctggcca ttgttaacac tagtgaggcc gactttaaca agctatctgg tgctattaat
       61 aatagcgctg gcgcggccga ccgaatggct aaagagatgc aggacaacct aaaagggcga
      121 ctggttgagc tgaaaagcgc cattgaaggg gttgcactac agttatacga agcaatgcaa
      181 ccggcactag aaaaggttgt cgcagctgta caaaagcttg ttgactggct agccaagcta
      241 agtccgggga tgcagacggc gataattgcc atcgcaggac tggcggccgc cattgggccc
      301 atactgatag tgctgggctt gctggcgcag ggtgtggggg cagtaatagg tgtcgtgggc
      361 tccctaacag ctgcactacc agcttttggg gcagtactcg ccgccttgac cggtcctatc
      421 ggcctagttg tagccgccat agccggcctt accgcagtgt ggctaatatg gggcgacgac
      481 atcaagcgga taaccagcga tgttgtctcg gcggtaaaag agtttttcac agacatgggt
      541 aaaaaaatag caggaacatg ggatgccgtg agggcgaaaa cgacagaaac ctggaacgcc
      601 attaagtctt ttctcaacac actatggagt aacttaaaaa gtacagttga aatcgtattt
      661 tccgctatta aatatagtat cgaatccata acacgtgcta ttcaaagtat actatcgaac
      721 atttggaacg ctataaggaa tttcttgtct gaaactttag acgcgattaa agttaagttt
      781 aacgaaattt ggaataccat taaggtgttc ttggaagaac tctggactgc gctaaaagat
      841 tttgcaatag ctatttttac atcgctgcag caaaacatcg aaactataac tttggctata
      901 caagccttaa ttactgatat ttggaacgcc ataaaagact tcttggatga aacatggaat
      961 actattagct ctacggcaga aagcgtgttt aacgctatta aagagctatt aaacactata
     1021 atgaatacga taaaaagtaa tacggaagaa gcatggaacg ctataaggag ttttttgtct
     1081 gaaactttag acgcgattaa agttaagttt gacgaaattt ggagtggctt aaaccagtct
     1141 accacggatg catgggaagc tatgaagagt acagtgaaag cgtcaataaa tgggattatc
     1201 ggcttcataa atgacatgat tagagcttgg aatgggatta gttttacagt cccgcgagtg
     1261 tcagttccct ttgtcgggac cttcgggggc tttagtgtcg gtactataca aatccccgag
     1321 ataccatttt tagccaaggg cggcgttgtt actaaggaga tgttgacggt cttgggggag
     1381 gcaggcccgg aagcggttat cccattgcca aaactacaac caatgttgtc cgcggcttta
     1441 acggccgcct taggtaaacc tggcggcggt gcaactattg attacgataa gctagcggcg
     1501 gcagtggcta agcatgttaa gcccagcata acgcaacaca atgtatttaa cagcccggag
     1561 ccattgacac cagcagaaac agctaggcag aatttaagag taagcagaac gcttgctatg
     1621 gaatgggggt tatagtttgt cacgaaaatt tgtatttaca aatagcaagg ggcagagtgt
     1681 cgaatttacc aacgatgccc ctttatttat gaaaacttac cacggtaccg gcggcagtcc
     1741 tgtggatatc catacgcaaa aaagtccaca ccaggacgga gaagcgctca ttaatacaac
     1801 tttggaaatg cgggatatac agctggtggt tgctatccta ggcaacagta cttccgaggt
     1861 tatgcaacac aggcggaggc tgtcgcaggt ccttaaccct aagcttgggc ggggtaagtt
     1921 gagatttgaa agcgagacag gcgcacatga aatagacgta cagccaaacg gagcgccagc
     1981 cttccccggc ggcgaggcaa gaggggcatt gtaccaagag tgcctgattg agctattggc
     2041 acctaatcca ttttgg
//
LOCUS       DASRMS010000061        27690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_13718, whole genome shotgun sequence.
ACCESSION   DASRMS010000061 DASRMS010000000
VERSION     DASRMS010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 27690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27690
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13718"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..360
                     /locus_tag="VFC74_02500"
     CDS             181..360
                     /locus_tag="VFC74_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24240.1"
                     /translation="MTIKRAWTTNWKTGKRVYPKKGKCIIILRTPKEKEKYRKWKEQN
                     EKEKSLVAARLQSKN"
     gene            complement(667..743)
                     /locus_tag="VFC74_02505"
     tRNA            complement(667..743)
                     /locus_tag="VFC74_02505"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(707..709),aa:Ala,seq:ggc)
     gene            complement(1033..1488)
                     /locus_tag="VFC74_02510"
     CDS             complement(1033..1488)
                     /locus_tag="VFC74_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HZK24241.1"
                     /translation="MNIDDYRKIIATAIEFEIESYEFYQGVAEKTTDNNIKAIFASFA
                     TEELAHKNFLEDFLQGDARSLAIKTQPDYKVADTVAMPKLTVEMQPAEAIALAMKKEE
                     EAMKMYTSLANHADSPEHKKLFADLAAMEKNHKVELENIYVSTAFPEVW"
     gene            complement(1611..1961)
                     /gene="rsfS"
                     /locus_tag="VFC74_02515"
     CDS             complement(1611..1961)
                     /gene="rsfS"
                     /locus_tag="VFC74_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HZK24242.1"
                     /translation="MSQEVHRLLALIVNAAEDKKAVDLTILDIGKVSFVADYFVIATG
                     TTKTQVHAIADSIMEKLKESDDALLHREGYQEGLWILLDYGSVVVHIFQPEQRNFYHL
                     ERLWAHAPKVTGLS"
     gene            complement(1974..3098)
                     /locus_tag="VFC74_02520"
     CDS             complement(1974..3098)
                     /locus_tag="VFC74_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZK24243.1"
                     /translation="MPAYYKVKTKRRRLKKGRLIFLLSICAFILLLFFTAGFLGDLQE
                     LQDRSAWAKKLPKAAKDKPQHFLLYTLDKEAVTALYLLSLPPDQQVVHAVQIPTDTLL
                     NSNSEDETLVEEAYSQAGREGLLKSIADLLQTDIHAFVEINEDHLADVTGALKLTYAE
                     TNATKDVLNYINDAELSAPQQTERRRQVLTTVADQIIKGNLLQEVLRLRKASPLVASN
                     LTWRKLLSLTKSFGEAKFSETTKIYALPGSVAVAAERNYWLADTDSIPSLMVWLDSDS
                     PNIPREQITVEILNGCGIAGLANQVAQLLREEGFTVTRVANADNFSYERSQVISRISE
                     MEPAKEVAILLPNAQLIKNEVSDAAVVVTVIVGKNYHSSE"
     gene            complement(3116..3670)
                     /gene="yqeK"
                     /locus_tag="VFC74_02525"
     CDS             complement(3116..3670)
                     /gene="yqeK"
                     /locus_tag="VFC74_02525"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: protein motif:HMM:TIGR00488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HZK24244.1"
                     /translation="MSELNLQQQLRAILSPKLYAHCAGVAQTAADLAVLLGCDCQKAR
                     LAGWLHDCAREWPAEKLFAFAAHNHIAVDRYTQRAPVILHAPVGAVVAKKWGITDREI
                     LAAIRKHTLGEPEMTTLEQLVYLADKIEPHRSYPGVEALRDLAQQNFQQALIQASANS
                     ISYLLEKRQLVHPQALAFWNWLVS"
     gene            complement(3800..4051)
                     /locus_tag="VFC74_02530"
     CDS             complement(3800..4051)
                     /locus_tag="VFC74_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HZK24245.1"
                     /translation="MAKTIYIGNIPWSATEDQLKEFFSEQGQVYSARIITERATGRSK
                     GYGFVEVDDEDVERIVEMNGVELEGRKLVINEAKPRPEY"
     gene            complement(4114..4761)
                     /gene="nadD"
                     /locus_tag="VFC74_02535"
     CDS             complement(4114..4761)
                     /gene="nadD"
                     /locus_tag="VFC74_02535"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HZK24246.1"
                     /translation="MTIQLPECMEELSVGIMGGTFDPIHLGHLVIAEEVRLQYNLDKV
                     IFVPTGYPPHKVNRKVTDPEHRYLMTVLATINNPFFTVSRVEIDRAHALTYTIDTLRF
                     FNNYYNGQAGIYFITGADAIMDILNWKDYQELFSLCHFIAVTRPGYNLNLEETIGKVY
                     PEALSRIEVLEIPAMQISSTLIRQRVVTGKPIRYLTPDAVTQYIFKNGLYRNERE"
     gene            complement(4778..6064)
                     /gene="obgE"
                     /locus_tag="VFC74_02540"
     CDS             complement(4778..6064)
                     /gene="obgE"
                     /locus_tag="VFC74_02540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HZK24247.1"
                     /translation="MFVDRAKIYVKGGDGGNGIVAFRREKYVPFGGPDGGDGGRGGDV
                     IFVVDPNLRTLLDFRYQQHLRADRGDHGKGSNKHGRGAKDKFVKVPPGTQVRDAESEA
                     LLADLTDEGQSFVVAKGGRGGRGNTRFSSPADKAPRYSEKGEPGEERWVWLELKVIAD
                     VGLVGFPNAGKSTFLSRVSAARPKVADYPFTTLSPNLGVVDLEGVEPFVVADIPGLIG
                     GAHEGVGLGHHFLRHVERTRLLVHIVDAAAVDGRSPLADYQQINEELRLYDERLASLP
                     QVVVANKTDLPQAAEGLVELQRELGEENVFAISAVTGTGIQEVLYHTAQLLTALPPET
                     PLAEEPAIFTPGDEDEEEEKVTITKENDIFTLHGPRLEKLVAMTDFNNEDGIKRFQRI
                     FRLQGHEDALKAAGARNGDTVRIRKLEFTFWQEEDN"
     gene            complement(6096..6629)
                     /locus_tag="VFC74_02545"
     CDS             complement(6096..6629)
                     /locus_tag="VFC74_02545"
                     /inference="COORDINATES: protein motif:HMM:NF026040.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HZK24248.1"
                     /translation="MNDVIEAMDKLLEHYRHDFLNVLQVAGGLAQLHKTERLLAYISQ
                     ASAEVQQFGHFISCGDSRFALIVLENLLQTLNGHYVLQVKGRMPLLSPDILQALPDTL
                     TAVLDCLPKVNDYTVSLYLKGDEQPQLRLSLLEDLEIDWQQAQAAAERHDLQAGLDKV
                     KGEFLLLLDKRDSTGEK"
     gene            complement(6767..7057)
                     /gene="rpmA"
                     /locus_tag="VFC74_02550"
     CDS             complement(6767..7057)
                     /gene="rpmA"
                     /locus_tag="VFC74_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HZK24249.1"
                     /translation="MMLMNLQLFAHKKGVGSSRNGRDSNPQYLGVKRASGQAVLAGSI
                     LVRQRGTKIHPGSNVGRGKDDTLFALVDGVVAFERKGRDKKQVSVYANGEAV"
     gene            complement(7071..7394)
                     /locus_tag="VFC74_02555"
     CDS             complement(7071..7394)
                     /locus_tag="VFC74_02555"
                     /inference="COORDINATES: protein motif:HMM:NF016233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HZK24250.1"
                     /translation="MIRVCVKRVAGQVTKLVVSGHAGSAPHGEDIICAAVSVLVQTYL
                     FSLQRLLQLDVAAEILDGYFSLILPTGLAPAVQEKVSLLTESTLVGLAEIDKSYPGFL
                     QVSEE"
     gene            complement(7398..7709)
                     /gene="rplU"
                     /locus_tag="VFC74_02560"
     CDS             complement(7398..7709)
                     /gene="rplU"
                     /locus_tag="VFC74_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HZK24251.1"
                     /translation="MYAIIETGGKQYRVEEGSILDVELLDGEIGTELGFEHVLALKKD
                     DKLQVGQPYLTDVKVRGKVLEHGKGKKIVVFKYKAKKNYRRKQGHRQPYTRVLIEQIE
                     A"
     gene            complement(7826..9508)
                     /locus_tag="VFC74_02565"
     CDS             complement(7826..9508)
                     /locus_tag="VFC74_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HZK24252.1"
                     /translation="MQKKIIINCDNKQTRVAMLEDNKLVEIYLERPVYQRVVGNIYKG
                     VVANVLPGMQAAFVDIGLERNAFLYVDDAIVSNEETEKGNKNKTIEQLLRPGEEIMVQ
                     VIKEPFGSKGARLTRQITIPGRHLVLMPWSKNTGVSRRIDDTAERERLRQLASQLRPE
                     DMGLIVRTVAEGEDVNAFQQDLTFLLSLWERTERRYQQKSAPYMIYRDLDLIYRILRD
                     LFTDQIDQLLIDTRYEYEKILETLDVIDSHLKERVELYHGDTPIFDVYGIETEIEKAL
                     SRTVWLSCGGYLVFDHTEALTVIDVNTGKYIGKTNLADTVLKTNLQAAEEIIRQIRLR
                     DIGGIIIIDFIDMETEEHGQQVLAVLAEKIKQDRTKSHVLGMTGLGLVQMTRKKARQG
                     LDAVLQQSCPYCGGRGKVLAADLISARTERQLRSFLEGLDAEAILVELHQSVAGLLIG
                     SSGSSLRKLEEETGKIIYIRGSETIHVEKYKILAVGSQSEVRAQAFPVAVGEMHQVKV
                     EEPHTNNPYDGIARISGFVIDIQAGGAYVGEEVSVEILEVSKTFARAKVVTN"
     gene            complement(9480..10181)
                     /locus_tag="VFC74_02570"
     CDS             complement(9480..10181)
                     /locus_tag="VFC74_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR03936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03936 family radical SAM-associated protein"
                     /protein_id="HZK24253.1"
                     /translation="MRIWIKFTKEQPLRFLSHLDLMRLWQRAIRRARLPVVYSQGFNP
                     HPKMSFAAALPVGVTSEAEYLDVHFDKEITDVDINSLQSVLPNGLTIYGWRPVPTGTA
                     ALMALVGAHKWQTSLESVSAVQGRIEQLLQDTELWVERQSKKGVKVVNLRPYIYQLKA
                     AAATNSLEMFLASGSAGGAKPREVLALLELQSHSDVRCTETFLKVNDFLQSPLAVFLE
                     EKEVSVNAKEDYYQL"
     gene            complement(10165..12027)
                     /locus_tag="VFC74_02575"
     CDS             complement(10165..12027)
                     /locus_tag="VFC74_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03960 family B12-binding radical SAM
                     protein"
                     /protein_id="HZK24254.1"
                     /translation="MKKDYQQYFWQHILPRTEKPTRYLGNEVNAIVKDHQQAAMRVVL
                     AFPDLYEIGMSHLGLKILYHIINQEEHWLAERVFMPAPDAEELLREASFPLCSLETVT
                     PLADFDLVGFTLQYELSYATILQMLTLANIPLTAADRGEQAPLVVGGGPGAFNPEPLA
                     DFFDFFLLGDAEEALPQILTLLTENKAAPRSEKLRLLAMITGVYVPSLYQEKRSTSGE
                     YLGTVPITEDLPTRVQRAVVANLDQAPYPTEFIVPYGGIVHDRLVLELFRGCTRGCRF
                     CQAGIIYRPVRERKVETLKELARQMVHATGYEEMALSSLSSGDYSAIEQLVRELGIDL
                     AQAGVSLSLPSLRLDSFSSDLAEQVQRVRKSGLTFAPEAGTQRLRDVINKNITEEDLL
                     QAVQEAFAAGWTGVKLYFMLGLPTETDEDLLGIAELARKVVNVYKNSGGRGRLRVTVS
                     TAVFVPKAHTPFQWVGQISQQEMLRRQALLKQALRIPGVHFQYHDAQTSMLEAAIARG
                     GRELVPVISRAADLGCKLDGWDEHFSYEHWEQAFRENGLEVANCAQKDYTEQEPLPWD
                     HLEAGVSKRYLRREYQHALAGEVTGDCREGCLGCGMTLLLPEEEKGVCTRADLD"
     gene            complement(12158..13042)
                     /locus_tag="VFC74_02580"
     CDS             complement(12158..13042)
                     /locus_tag="VFC74_02580"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZK24255.1"
                     /translation="MKLFRLGGITFSFHWVFILLLFLLALFGYLGETLILFGLVLAHE
                     LVHMFTARAQGLEVSEVELFPFGGVAKIEDVLELDPQVESVVSLAGPLFNFALVALAL
                     LLYANVPEWQQNELLLFFIRSNLTLGFFNLLPALPLDGGRILRARLCGILGFQQATEL
                     AIRISQLLALLLFALTLYLFYLGDFHVTLLAAATFLYFASEKEKTAAMYAFIRGLGRK
                     KRVLYEQGVMPLVSLLSLRDTLMKDILRRFAMKKFHRVLVVTREGQVLGEVMEDEVVE
                     TIVREGLYTTIESALRHK"
     gene            complement(13058..13855)
                     /locus_tag="VFC74_02585"
     CDS             complement(13058..13855)
                     /locus_tag="VFC74_02585"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZK24256.1"
                     /translation="MKDNRRWQAPQFGLRERLAQRRAKSSTSYYNEYTQDYKQPQALD
                     LPYLLTKHWRENQFQKTVAAIIVIICLGAVSLFNTPLTNRVVDWAYQLTVKQTNPTDI
                     IAAAQPVLASLRDFSWRQQSTTEDPLANETVTTLDGMVAPVNGELSSPYGARIITGSN
                     EMEMHYGIDVSAEPGSPVYAALAGTVTVVDPAHPVLGETIYLQHVDDTVTIYGRVTGS
                     LVIAGDQVAQGAEIAKIAAAEGESHLHFEVWQEKQPVDPEPYLKQTN"
     gene            complement(14006..15112)
                     /gene="rodA"
                     /locus_tag="VFC74_02590"
     CDS             complement(14006..15112)
                     /gene="rodA"
                     /locus_tag="VFC74_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HZK24257.1"
                     /translation="MFDRRLLRNFDWLLLLTVCAIVAISLLIITSTTINITGDPLYFA
                     KRQLIRFAVGFVAMLFILSIDYTYLHRFAPYIYLTVLALLIAVLFVGSDGGGAQRWID
                     LGFFELQPSEFAKLGIIISMARLLSAQEGDFEGMASLLPAFFHVALPMGLIFMQPDLG
                     TSLVFTAISFGMLYMAGAKVQHLMTYAGIGLAVGLPALWFTLKDYQKMRLIIFTNPYL
                     DPLNDGYQIIQSLIAVGSGGLVGKGLFAPGTQNALNFLLEQHTDFVFSAYAEATGLLG
                     SVLLVLLYAFLILRIIRLAAQAKDTFGMLVCIGVAIMMTFHILVNIGMTIGIMPVTGI
                     PLPFMSYGGSSLLMNMMAIGVVLNIGMRRQKLMF"
     gene            complement(15228..15494)
                     /gene="minE"
                     /locus_tag="VFC74_02595"
     CDS             complement(15228..15494)
                     /gene="minE"
                     /locus_tag="VFC74_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HZK24258.1"
                     /translation="MNLRAMLNALMGRQDSKDVAKERLRLVLVHDRASVSPEFLDKIK
                     EEMIQVISKYMEIDENQTQINMHHSEGTAVLEANLCIKSIRRNM"
     gene            complement(15509..16306)
                     /gene="minD"
                     /locus_tag="VFC74_02600"
     CDS             complement(15509..16306)
                     /gene="minD"
                     /locus_tag="VFC74_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013657107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HZK24259.1"
                     /translation="MGEVIVVTSGKGGVGKTTTTANLGVGLAMQNKKVVLIDADIGLR
                     NLDVVMGLENRIVYDLVDVVEGNCRTKQALIRDKRYENLFLLPAAQTRDKNAVSPEQM
                     KKLCDELKEEYDYILVDCPAGIEQGFRNAIAGADQAIIVTTPEVSAVRDADRIIGLLE
                     AAELEESRLLINRLRPEMVKRGDMMNIDDILEILAIELVGVIPDDERIIVSTNRGEPV
                     VADSNSLAGQAYRNVVQRILGEAVTFLDLNQNHSFFSRLKRLIGFGG"
     gene            complement(16338..17003)
                     /gene="minC"
                     /locus_tag="VFC74_02605"
     CDS             complement(16338..17003)
                     /gene="minC"
                     /locus_tag="VFC74_02605"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HZK24260.1"
                     /translation="MSNITVEFKGNKDGVTIYCLDHAAFSDIMSDLAERLKQRAAFFG
                     EAPVRIDVGQRELTEEETEALIDVVRQNSRLHLIAINSASDRPPAIAAARRQGETDDL
                     RLDGFKEGRALVIKRTLRSGQYIRFPGHVIVLGDVNPGAEIVADGDIYVFGTLRGIAH
                     AGVDGDRSASVVALRLAPTQLRIADIISRAPDDSELPEQPEYAYVSDLGVMIAAITTK
                     LGS"
     gene            complement(17104..18873)
                     /gene="mrdA"
                     /locus_tag="VFC74_02610"
     CDS             complement(17104..18873)
                     /gene="mrdA"
                     /locus_tag="VFC74_02610"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZK24261.1"
                     /translation="MTRSLQKRLRMFTFAVIFVALLLTARLAWLQIYKYDHYLGQAEN
                     NRLRDLPITATRGEIRDRNGISLATNRPGFSVSLLDLDRQKAAEVIDYLSETLEIEKE
                     EIQEKILQQQYKRFAPIRIANDVSAEIVAKLEEHAMDLPGVIIETQPVREYPLQTLAA
                     HVLGYVGVIRDYQYEAMKAEGYRFTDIIGQAGLESTYEQYLHGQDGILRIETDRFGNR
                     VRVLERTEPVGGNDLELTLDTRLQEIAARSLAQGIADLQKKGNTQASRGAVVALDPNT
                     GGILAMVSFPGFNPNTFFNDYKEIQADPNKPEINKAIQGTYPVGSTFKLVGTLAALEE
                     GVITEKSRVTCVGVKSFFGSDRRRCFNSTAHGHLNIIGALQKSCNIFFYEMGYRLGIE
                     RLAAYAHDFGFGKVTGLTDIRGEVAGVIDSKERRSNFMPGDVLTAAIGQGHAITPLQL
                     ANYAAILANHGTHYRPYLVEKALNGKGKVVYEAKPEVLNTLPYSEKNWELARTGMKAV
                     TEPGGTGAVLRTVPVKVAGKTGSAQVSAGKISHSLFVGYAPADNPEVALAVIVENGGL
                     GGDGGVPVAKLIFEEYFALAEMD"
     gene            complement(18870..19397)
                     /gene="mreD"
                     /locus_tag="VFC74_02615"
     CDS             complement(18870..19397)
                     /gene="mreD"
                     /locus_tag="VFC74_02615"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HZK24262.1"
                     /translation="MAALLIFVLFISSLVLQGSVLALAGPSGVQPDILLVVTVALALL
                     ADARRGAIVGLCAGLAQDILFGSPLGFFALSKMLVGTTAGLLSSDINKELTLSAMVLM
                     PIFTIISDLISFILLKLYQIQFPLPLLTYLKQVTLPHIAMHFLIMGIIYPFLFRAQKR
                     NLLFAESEAEEWSKL"
     gene            complement(19397..20281)
                     /gene="mreC"
                     /locus_tag="VFC74_02620"
     CDS             complement(19397..20281)
                     /gene="mreC"
                     /locus_tag="VFC74_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HZK24263.1"
                     /translation="MPVHVQKNNRKNNNLKIISLVSVLSVLLLLTGVTANRQGATWPE
                     EMLQYVVAPVQGVFQRLTRSVESVFSTARNYRLLRQENENLRQQLTEAVSLQTQIAEL
                     RQENDRLRSMLEYREASEYELITAEVIARDPSNWFKTITINQGTKDGIEPNMAVVTAE
                     GLIGNILSAAPNSAQVQLLTNASRRVSALVQRSREPGEVGVVEYDPNNTAYLRMKDLP
                     REANIQAGDTIISSGLGGVFPKGLLIGYVLETAEDEMGLTQYAQLQPAANFNRLEEVF
                     VVIPQSDLPVIPEEEENL"
     gene            complement(20308..21345)
                     /locus_tag="VFC74_02625"
     CDS             complement(20308..21345)
                     /locus_tag="VFC74_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HZK24264.1"
                     /translation="MRFVTKYFSRDIGIDLGTANTIVFVKGKGIVLREPSVVAIRRDT
                     GSLLEVGEEAKRMIGRTPGNIVAIRPMKDGVIADFDVTQTMLRHFINKAYKRTTMFKA
                     QVVVCVPSGVTEVEKRAVIDATKQAGAKEAFLIEEPMAAAIGAGLPVEEPTGSMIVDI
                     GGGTTEVAIISLGGIVTSRSIRVGGDEMDESIVQYIKKNYNLMIGERTAEEVKIKIGT
                     AYREDVEPKKMDIRGRDLVSGLPKTQEISAEEIVSALADPVASILEAIKITLEKTPPE
                     LAADIMDKGIVMTGGGALLQGLDKLIARETGMPVYIAENPVDCVALGAGKALGEIELL
                     RRVALAPRRMH"
     gene            complement(21440..22135)
                     /gene="radC"
                     /locus_tag="VFC74_02630"
     CDS             complement(21440..22135)
                     /gene="radC"
                     /locus_tag="VFC74_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HZK24265.1"
                     /translation="MTTARLTIKELPPSERPRERALLNGVAALSDAELLAILLRSGTA
                     QETAVQLAEKVLLKVGGITELPRSSREELQSIKGLGPAKAVTLLAAAELATRLSNRTR
                     YESVTVSSPSDAAGLVMEELRHKLREHFRVLLLDTKNKVLGMEDISIGSLNSSLVHPR
                     EVFRPAIRKACASVILIHNHPSGDPTPSREDVDVTKRLTEAGCLMGIEVLDHLVIGDG
                     RFVSFREKGLITR"
     gene            complement(22092..22724)
                     /locus_tag="VFC74_02635"
     CDS             complement(22092..22724)
                     /locus_tag="VFC74_02635"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HZK24266.1"
                     /translation="MTKFILASASPRRAELLRQIGLPFTVQVSNVDESTINEAEPAQL
                     AAQLALYKGQAVAEKVDEGVIIAADTIVNINGELLGKPATKQAAAEMLRRLSGKLHHV
                     LTGVAVISKPAGLVRTHVESTAVYLRKLTEAEIDWYVNTEEPYDKAGGYGIQGKAAVF
                     IEKIEGCYFNVVGLPLAALWQLLMAAGIKLGEGAGEYDNSAPDHQGAATK"
     gene            complement(22735..23256)
                     /locus_tag="VFC74_02640"
     CDS             complement(22735..23256)
                     /locus_tag="VFC74_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019099.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HZK24267.1"
                     /translation="MKTKKLTHLAMLITFALVIHTVESALPMPIPGAKLGLANVITLL
                     TFVLYGLGAAMFVSVARTLLGSMFAGTLLSVGFYLSFAGAIISTLIMAVGIYFWRRGK
                     ISMIAVSILGAVAHNTTQVVVASILTSTPRLIALYLPVPLVLALPTGFFTGLAVMYSQ
                     KALANVIAEIKRN"
     gene            complement(23461..25290)
                     /locus_tag="VFC74_02645"
     CDS             complement(23461..25290)
                     /locus_tag="VFC74_02645"
                     /inference="COORDINATES: protein motif:HMM:NF012581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HZK24268.1"
                     /translation="MLPAAAHEMNTTTETISSLLPLAAIMVPLVGALLTYWLGQRSDK
                     LRNACTVLVTLATITVVMALYPLLQKGAVEFILLRLEQFNIFLRVDFFSFVFAALISI
                     IWCLTTLHATAYMAHEHDQNRFFTCLLATLSSCLGFVLAGDLITLIIFYEAVTFLSHI
                     LVTHEHTADAMSAGNGMLYLGIIGSFFLLLGIYQLYTGVGTLEIRPLYDQIAQSGLNS
                     YWLLIYFTIGFGIKAGMTPTHIWLPKVDPVAPAPVSALLCALFIKTGVYGILRVVDMI
                     FTPTAVMHDLATLLPAMNFGYLLIWVGIVSMFLGALMALLSTHAKKLLAYSSISQMGY
                     IIMGIGAASYLGAEGAIGFAGALYHVVNHAFFKACLFLVVGAIYMITHELDITVVRGM
                     IKKVPLITVIFLIAFAGIGGIPGFNGYVSKTLLHHALVEAAAHSHSVPLAIAEKIFVL
                     TSAMTLCYFTKFFRGLFLGEVPEKYDKKRYSFTWPVYTALGTLATIIVAIGLFPHYLL
                     QKLIIPALSGFTFDPHAVEHLYAINVWNWHDIQGMIVIVGLAVILFWAMDRFHFFSIK
                     FPCWLSIDYLLYQPVRMMLRRMVAPLRQNRAHDKTRDYTSHTH"
     gene            complement(25634..26416)
                     /locus_tag="VFC74_02650"
     CDS             complement(25634..26416)
                     /locus_tag="VFC74_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024634.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZK24269.1"
                     /translation="MILPAVVSLGGLGLIFGSMLAFASQKFAIEIDPKIEQVLEVLAG
                     ANCGACGCAGCAQFAEEVVAGRASITGCTPGGSKVAACIAEILGAEAPEAQERCVALL
                     KCQGDCNTVKAKHLYHGIADCKAAIALNGGPSACSYGCVGLGNCVKVCPVDAITMGEN
                     GLPLIDVELCISCGNCVAECPRHVIEMVSTAAQPHVLCKSRDKGKYVRSVCERGCIAC
                     GLCVKKCPENAIYMENNVAVIDSSLCNNCGICVEVCPEHTIV"
     gene            complement(26429..27004)
                     /gene="rsxA"
                     /locus_tag="VFC74_02655"
     CDS             complement(26429..27004)
                     /gene="rsxA"
                     /locus_tag="VFC74_02655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HZK24270.1"
                     /translation="MTSILTIVFGAIFINNFVLSRFLGICPFLGVSKKVETSLGMGMA
                     VTFVMGMSSLVTWLLEHYVLAAYSLQYLRTIVYILVIAALVQFVELVIRKVSPALYQA
                     LGIFLPLITTNCAVLGVALLNVQLEYNLLESVVFGIAGALGFTLALVIMAGIRERLEL
                     VFIPKAMRGVPIALIVAGILSIAFMGFQGMM"
     gene            complement(27018..27608)
                     /locus_tag="VFC74_02660"
     CDS             complement(27018..27608)
                     /locus_tag="VFC74_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HZK24271.1"
                     /translation="MKELTKGLFRENPTFRLVLGMCPTLAVTTLAVNGLGMGLSTAAV
                     LVASNFMISLLRKLIPDKIRIPAYIIIIATFVTMVDMLLNAYQPELHSQLGIFIPLIV
                     VNCIILGRAEAFASKNPVVSSVADGLGMGLGFTFSLTILASIRELVASGTLFGANILG
                     AAYQPFKVFASPPGAFIALGLLMALMNILAKKFRLE"
BASE COUNT         7835 a         6854 c         5634 g         7367 t
ORIGIN      
        1 actatatgtt gtgttagtaa aatatacatg caccatatat tgtttgtttt ttttgtaaat
       61 tcagtattct tgttgtaagt catagtgact gcaaatatgg atgctaccag cagtacatac
      121 tgatcccctt gtttcagcca catggttttc acaagaaaat aaagtaaaca ggtgatgctc
      181 atgacaatta aacgtgcgtg gacaaccaat tggaaaactg gaaagcgtgt ttatcctaaa
      241 aagggaaaat gtatcatcat ccttcgcacc ccaaaagaaa aggaaaaata tcgcaagtgg
      301 aaagaacaaa atgaaaagga aaagagccta gttgcagcta gactccaatc caaaaattaa
      361 ataagttttt tgtgacaaaa agtaggcttt tctggtagcg agtctacttt ttgtttatta
      421 taacttatta tattatcaga aatacctatt ttgtcaacaa tggcttacaa tcaaataaat
      481 tcaaataggt agcaattagg gtcaaaaaca tggggttaat tcgatattag caagttttaa
      541 gagtagtttg tgtgtatgct aatgtattta ccctagtgag gataaaaaac gttttaaggg
      601 gctttttagc gtgttttaaa acacaaaaaa ccacaagaaa taatctcttg tggttaaact
      661 tttatttggt ggagacgaac gggatcgaac cgtcgacctc ctgaatgcca ttcaggcgct
      721 ctcccaaact gagctacgtc cccatgcaaa aacaatatta acataaatct taatactaat
      781 caagtttaaa tttttaccat caaaaaatca gcgatgttta tggtagtatt tctaccttga
      841 tagctctccc aactgagcta catccccatt tgcagtacaa aatacattat actgattttt
      901 cttggtcgtg tcaactctat ctggtcggta aatactggct ggaggtattg gtgccaagct
      961 ttttacaggc cacgctttaa cagaatgtgc tcgacgattc tatgccgcaa gcgtgagaca
     1021 gcaaaagctg ctttaccata cttcagggaa tgctgtacta acatagatgt tttcaagttc
     1081 tactttatgg ttcttttcca tggctgccaa atcagcaaag agctttttgt gttcagggct
     1141 gtccgcatga ttagccaggc tggtatacat tttcatggct tcttcttctt ttttcatggc
     1201 cagcgcgatg gcctcagcag gctgcatttc cacagtaagt tttggcatag ccacagtatc
     1261 cgccactttg taatccggct gtgttttgat ggcgagagac ctggcatcgc cttgcaagaa
     1321 gtcctcgaga aagtttttgt gagctaattc ctctgtggca aaggaggcga aaattgcttt
     1381 tatgttgttg tcagtagttt tctctgcaac accttggtaa aactcgtaag actcaatttc
     1441 gaattcaatg gcagtagcaa taatctttct gtaatcatca atgttcaata tcatgtccct
     1501 cccgtttatt atgacgctta cttactacta tagtaagtaa gcaagaataa caaatgaaca
     1561 aatcattaaa tgaaacttat aaaaaataca gctaaactgg tggtattgtt ttaggagagg
     1621 ccagttactt taggagcatg ggcccagagg cgttcgagat gataaaaatt acgttgttca
     1681 ggttgaaaga tgtgaacaac gactgaacca tagtctaata ggatccacag accttcttgg
     1741 tagccttctc tatgtagtaa agcatcatcg ctttctttta gcttttccat gatgctgtca
     1801 gcaatagcat ggacttgggt ttttgtggta ccggtagcaa tgacaaaata gtcggcaaca
     1861 aaagatactt tgccaatgtc taagatagtt aaatccacgg cttttttatc ttctgcggca
     1921 ttaacaatca gtgcgagtaa cctgtggacc tcttgactca acagaacccc tccttattcg
     1981 ctactatgat agtttttccc cacgatgact gttactacta ctgctgcgtc acttacttcg
     2041 ttttttatta gctgcgcatt tggcaagaga atagctactt ctttggccgg ttccatttca
     2101 ctgatacggc tgatgacctg ggagcgctcg taggaaaaat tatcggcatt ggctacacgt
     2161 gttacagtaa agccttcctc gcgcagcagc tgggccactt gattggctag tcctgcgatg
     2221 ccgcaaccat ttagtatttc tacagtaatc tgttcgcggg ggatattggg gctgtcacta
     2281 tccagccaca ccatcagtga cgggatacta tcggtgtcag caagccagta gtttctttca
     2341 gcagccacgg ccacactacc tggcaaggca tagatctttg tggtttcact aaattttgcc
     2401 tcgccaaatg atttagttaa gctaagcagc ttacgccaag ttaaattgct ggccaccagg
     2461 ggtgaagctt tacgcagacg cagcacttcc tgtagtaaat tacctttgat gatctggtca
     2521 gcaactgtgg ttaacacctg acgtcgtcgt tctgtttgct gtggtgcaga taattctgcg
     2581 tcattaatgt agttcagcac atctttggtg gcgtttgttt cggcataagt cagcttgagg
     2641 gcaccggtaa cgtcagccaa atggtcctcg ttaatttcta caaaagcatg aatatctgtt
     2701 tgcagcaaat cagcaataga tttaagcaaa ccttcccggc cggcctgact ataggcctct
     2761 tcaaccaagg tctcatcctc gctgttgctg ttaagtaaag tatcagttgg gatttgcaca
     2821 gcatgcacta cttgctggtc gggaggaagc gatagcagat acaaggccgt tactgcctct
     2881 ttgtctaaag tatagaggag aaagtgctgc ggcttgtcct ttgccgcttt gggtagtttt
     2941 ttagcccaag ctgagcgatc ctgcagctct tgtaaatcac ctaaaaagcc tgcagtaaaa
     3001 aatagcagca ggataaaagc gcagatagat aaaaggaaaa ttaagcgacc tttttttagg
     3061 cgccgacgtt tagttttcac tttatagtaa gcaggcactt tttacacccc tttcgctaac
     3121 tcactaacca gttccaaaaa gcaagtgctt gcgggtgaac tagttgacgc ttctctagaa
     3181 gatagctgat ggaattggct gaggcttgga ttaaggcttg ctgaaaattt tgctgtgcta
     3241 aatcacgtag tgcttcaaca ccaggataag agcggtgcgg ttcaatctta tcagctaggt
     3301 agactagctg ctccaaagtg gtcatctcag gctcacctaa agtatgctta cgaatagctg
     3361 ccagtatctc tctgtctgta ataccccatt ttttagccac aactgctcct acgggagcat
     3421 gcagaataac tggtgctcgc tgcgtatacc tatccacggc aatgtggttg tgggccgcga
     3481 aggcaaaaag tttttctgcc ggccactccc gggcacaatc gtgcagccag ccagctagac
     3541 gtgctttctg gcagtcgcag ccaagcagca cggccaaatc agccgctgtt tgcgctaccc
     3601 cagcacaatg ggcataaagt ttaggtgaga ggattgctcg gagctgttgt tgtaaattaa
     3661 gctcagacat tttattatca ccgctaagct atattcgcta aaaaggtaag gttttcctgc
     3721 cacagctggc aggcaagaga taaattcact gagagtgcaa aaaaggcaac attcgttgcc
     3781 ttagtgcgtg cttagtaatt taatattcag gacgaggttt agcttcatta atgactagct
     3841 ttctgccttc taactccacg ccgttcattt ccacaatgcg ttccacatcc tcgtcgtcga
     3901 cctccacaaa accgtatcct ttcgatcggc ctgtggcgcg ttccgtaata atacgggcac
     3961 tgtaaacctg accttgttca ctaaagaatt cctttaactg gtcttctgtc gctgaccagg
     4021 gaatgttacc aatataaatg gtttttgcca cgctcgcgct cacctcactt tccggtctta
     4081 tctgtagtat ctgaaaaact ggttgattta tacttattct cgctcgttac gataaaggcc
     4141 gtttttaaag atatactgcg tcacagcatc gggagttaaa tatctaatag gttttccggt
     4201 aactacacgc tggcgaatga gggttgaaga aatttgcatg gctggtatct ccagtacttc
     4261 gatgcgcgat aatgcttctg gataaacttt accaatggtt tcctctaaat ttaagttgta
     4321 gccaggccta gttacagcaa tgaagtggca aagagaaaac aactcttggt agtctttcca
     4381 gtttaaaatg tccataatcg catcggcacc cgtgataaaa tagatacctg cttgcccatt
     4441 gtaatagttg ttaaaaaaac gcagtgtatc aatggtatag gtaagtgcgt gtgcacggtc
     4501 tatctccacc ctagaaacag taaaaaatgg attgttaatg gtggctaaaa ccgtcatcaa
     4561 ataacggtgt tcaggatctg taactttacg attcactttg tgcggcggat agccggtagg
     4621 tacgaatata actttatcta aattgtattg gagtcgtact tcttctgcaa taactagatg
     4681 acctaaatga atgggatcga atgtcccacc cataatgccc acagaaagct cttccataca
     4741 ctcaggtaac tgtattgtca ttgtccccac cccgtaatca gttatcctct tcctgccaaa
     4801 aggtaaattc aagcttgcga attcttacgg tgtctccatt gcgagcaccg gccgctttta
     4861 aagcatcctc atggccttgc agacggaaga tgcgttgaaa ccgtttaata ccatcttcat
     4921 tattaaagtc agtcatcgcc actagttttt ccagacgcgg gccatgcagt gtaaagatat
     4981 cattttcttt ggttatcgtt actttttcct cttcctcatc ctcatcacct ggcgtaaaga
     5041 ttgcaggctc ttcagccagt ggtgtttcgg gcggcagtgc tgttaaaagt tgtgctgtat
     5101 gatagagaac ttcttggata ccagttccag taacagcaga gatggcaaag acattttcct
     5161 cacccagttc acgctgcaat tctaccaaac cttcagccgc ttgcggcaaa tcggttttat
     5221 tggcgaccac tacttgtggc aaactagcca aacgctcgtc atataaacgc agctcttcat
     5281 taatctgctg ataatccgcc agcggactgc gcccatccac ggcagcagca tcaactatat
     5341 gtactaaaag acgtgttcgc tctacatgac gtaagaaatg atgtcctaag ccaacgcctt
     5401 cgtgagcacc tccaattaac ccaggaatat cagcgactac aaaaggctct actccctcta
     5461 aatccacgac acctagattg ggcgacagcg tagtaaaggg atagtcggct actttggggc
     5521 gggcagcaga aacacgggaa agaaaggtag acttgccagc attggggaag cccaccagac
     5581 cgacatcagc aattaccttt agttctaacc aaacccaacg ttcctctccc ggttcaccct
     5641 tctcactata acgtggtgct ttgtcggcag gactagagaa acgagtgttg ccacggccgc
     5701 cacgcccgcc tttggcaacc acaaacgatt gaccctcgtc tgttaagtca gctagtaaag
     5761 cttcactttc ggcatctcta acttgtgtgc ctggcggcac cttaacaaac ttatctttgg
     5821 cgccacggcc gtgtttattg ctgcccttgc catggtcgcc gcgatcggca cggagatgct
     5881 gctgatagcg aaaatctaaa agcgtgcgca aattaggatc gactacaaaa attacatcac
     5941 cgccgcggcc accatcgccg ccatcgggtc cgccaaaagg aacatacttt tcacgacgga
     6001 aagcaacaat cccgtttcct ccgtcgccgc ctttaacata aattttggcg cggtcgacaa
     6061 acaataatac cactccttat taacttctct ctgctttatt tttctcccgt cgaatcgcgc
     6121 ttatccaaaa gtaataaaaa ctctcccttt actttatcta agcctgcttg taaatcgtgg
     6181 cgttcggcgg ctgcttgagc ctgttgccag tcaatttcca aatcctccag caggctcaaa
     6241 cgcagttgtg gctgctcgtc gcccttaagg taaagagaaa ctgtatagtc gttgactttg
     6301 ggcagacagt ctaagacagc tgttaatgta tctggtaaag cttgcaaaat atcaggcgat
     6361 aaaagcggca ttctgccttt tacctgcagg acataatgcc cattgagcgt ctgcagcaga
     6421 ttttctaaaa ctattaaggc aaaacggcta tcgccacaac tgataaaatg accaaattgt
     6481 tgtacctcag cactggcttg actgatataa gcaagcagcc gctctgtttt atgtaactgt
     6541 gctagtccac ctgccacttg cagaacattt agaaaatcat ggcggtaatg ctccaggagc
     6601 ttatccatcg cctcaattac atcattcatg acaatctcct cacttaaata acatctcagt
     6661 ataattcggg agtgacattt aaaatccttt attttcctta aaaccttaca gcttactaat
     6721 aacaaataaa ccggagtgat cactccggtt tattgtaatt tgctttttaa acagcttcac
     6781 cattagcgta aacgctgacc tgttttttat cacggccttt acgttcaaag gcaactacac
     6841 catctacgag ggcgaacagg gtgtcatctt tacccctacc tacattggag ccgggatgaa
     6901 ttttggtgcc acgttgacgc actaaaatgc tgccagctaa aacagcctgc cctgaggcac
     6961 gtttaacacc taaatattgg gggttactgt cacgaccgtt acgggaactc cccacaccct
     7021 ttttatgtgc aaataattgc agattcatta acattatcag agttcacctc ctattcttcg
     7081 ctcacttgta aaaagcccgg ataactttta tcaatttctg ctaaacctac cagcgtactc
     7141 tccgttaaga gtgatacttt ctcctgaact gcgggagcta aaccagtagg cagaataagg
     7201 ctaaagtagc catctaggat ctctgctgcc acgtctaact gcagcaaacg ctgcaagcta
     7261 aaaagatatg tctgcaccag cactgaaaca gccgcacaga tgatatcctc accgtggggt
     7321 gcgctgccgg catgaccgct aactactagt tttgttactt gaccagccac ccgtttaacg
     7381 cacacacgga tcattcttta cgcctcgatc tgctcaatga gtacccgagt atagggctgg
     7441 cggtgaccct gtttacgccg ataattcttt ttcgctttat acttgaagac aacaattttt
     7501 ttgcctttgc catgctctag cactttcccc cggactttaa catcagttaa atacggttgc
     7561 ccaacttgga gtttatcatc ttttttgagt gctaaaacat gttcaaagcc taattctgta
     7621 ccgatttctc cgtcgagaag ctctacatct aaaatggagc cttcctcgac acggtattgt
     7681 ttaccgcctg tttcaataat tgcatacatg aaaataaatc accttcctat ctagactcgc
     7741 cggtaaaggt accctacggt ttacaacccc acccgagcgg ttacgatggt cacttagtta
     7801 ttctagcata gcagcatatt gtttgtcaat tcgtaacaac tttagcacgg gcaaaagttt
     7861 tcgagacttc taaaatctct acgcttactt cttccccgac ataggcacct ccggcctgga
     7921 tatcaatgac aaaaccactg atacgtgcta tgccatcata tgggttattg gtatgcggct
     7981 cctctacctt gacctggtgc atttctccca cagcaacggg gaaggcctgt gctcgcactt
     8041 ctgactgact tcccaccgcc agaattttat atttctctac atgaattgtc tcactgccgc
     8101 gaatatagat aatcttacca gtctcttctt ctaacttacg cagggaggag ccactggagc
     8161 caatgagcag tcccgctaca ctttggtgca gctccaccaa aatagcttcg gcatcaagcc
     8221 cctctagaaa gctgcgcagc tgacgttctg tacgcgcact gatgagatca gcagctaaca
     8281 ctttaccgcg gccaccgcaa tatggacagc tctgctggag cacagcatct aaaccctgcc
     8341 gcgccttttt acgggtcatt tgcaccaaac ccaaaccggt catgcctaaa acatgggatt
     8401 tggttctgtc ttgtttaatt ttttccgcca acacggccaa aacctgctgc ccatgctctt
     8461 cagtttccat atcaatgaaa tcaataataa tgatgccgcc aatatcccgc aggcgaattt
     8521 gccggataat ttcttccgcc gcctgtaaat ttgttttcag tactgtatcc gccaggtttg
     8581 tcttgccgat atatttacca gtattaacgt cgatgacagt taaagcttcc gtatggtcaa
     8641 aaactaaata cccaccacaa cttaaccaga ccgtccggga aagcgccttt tctatttccg
     8701 tctctatgcc ataaacatca aaaatcggag tatccccgtg ataaagctcc acacgttcct
     8761 ttaaatgaga gtcaatgaca tctaatgttt ccaggatttt ttcatattca tagcgagtat
     8821 ctatgagcag ctgatcaatt tgatcagtaa aaagatcacg taaaatacgg taaatcaaat
     8881 cgagatctcg atatatcatg tagggcgctg acttttgttg gtaacgtctt tctgtgcgtt
     8941 cccagaggga taacagaaag gtcaaatcct gctggaaagc gttaacatct tctccttcgg
     9001 ccactgtccg cacaattaag cccatatctt ccgggcgtaa ttgagaagct agctggcgca
     9061 atctttctcg ctcagcagta tcatcaatgc gccgtgagac accagtattc tttgaccaag
     9121 gcatgagcac caaatgacgg ccaggaatgg taatctgcct cgtcagacgc gcacccttgc
     9181 tgccaaaggg ttctttaatt acctgcacca tgatttcttc accagggcgc aatagttgtt
     9241 caatagtttt atttttatta cctttctctg tttcctcatt gctaacaata gcatcgtcga
     9301 cataaagaaa ggcgttacgt tctaaaccga tatctacaaa tgcggcttgc atgcccggca
     9361 agacgttggc cacaacaccc ttatagatgt tgccaaccac gcgctggtag acaggacgct
     9421 ccaaatagat ttccaccagt ttattatctt cgagcatcgc cacacgagtt tgcttattgt
     9481 cacaattgat aataatcttc ttttgcatta acagacacct ctttttcttc caaaaacaca
     9541 gccagcggtg actgtaaaaa atcattaacc ttaaggaatg tttctgtaca ccgcacatca
     9601 ctatgtgatt gtagctctaa taacgctaag acctcccgcg gtttcgcccc gccagcactc
     9661 cctgaggcta aaaacatttc aaggctattt gtggctgccg ccgcttttag ctgataaata
     9721 taaggtctca ggttaaccac ttttacacct tttttacttt gccgctctac ccataactct
     9781 gtgtcttgca gcaactgttc tatgcgccct tgtacggcag agacagactc taaagacgtt
     9841 tgccacttat gtgctcctac taacgccatc aatgctgctg tgcccgtagg cacagggcgc
     9901 cagccataaa tagtcagacc gttaggcagc acactctgta agctattgat atccacgtct
     9961 gtaatctctt tgtcaaaatg aacatccaaa tattcagcct cactagtcac acctacgggc
    10021 agcgctgccg caaacgacat tttgggatga gggttaaagc cctgactata caccaccggc
    10081 agacgagctc gacgaatggc ccgctgccac aaccgcatta aatctaaatg tgataaaaac
    10141 cgcaagggtt gttctttagt aaacttaatc cagatccgca cgtgtacata ctcctttttc
    10201 ttcctctggc aacagtaatg tcatgccgca gcccaggcat ccttcacggc agtcccccgt
    10261 aacctcgccg gctaaagcgt gctggtattc tcgccgcaaa taacgtttgc tcacgccggc
    10321 ctcgagatgg tcccagggca gaggttcctg ttcagtataa tctttttgcg cacaatttgc
    10381 cacttccaac ccgttttccc taaatgcctg ttcccagtgc tcgtaggaaa aatgctcgtc
    10441 ccagccatca agtttacagc ccaaatcagc agcacggctg attaccggca ctagttcacg
    10501 tccaccgcgg gcaatagcag cttccagcat actggtctgc gcatcgtggt actgaaagtg
    10561 cacaccggga atccgtagtg cttgttttaa gagtgcttgg cgccgcagca tttcttgttg
    10621 tgaaatctgt cccacccatt gaaacggtgt atgcgctttg gggacaaaaa cagcagtgga
    10681 gacggtaaca cgcagccgac cacgacctcc agagttttta taaacattaa ctactttgcg
    10741 tgccaattca gcaataccta aaagatcttc atccgtttca gttggtaatc ctaacatgaa
    10801 gtagagcttc actcccgtcc agccggcagc aaaagcctct tgtaccgcct gcagcagatc
    10861 ttcttctgtg atattcttgt taattacatc ccgcaggcgt tgtgtccctg cttccggagc
    10921 aaaggtcaga ccgctttttc tgacacgctg cacctgttct gccaggtcgc tggaaaagga
    10981 atctaaacgt agagaaggca aagaaagcga gacgcctgct tgtgctaaat ctatgcccag
    11041 ctcacgaacc aactgctcaa tagcactata gtcaccgctg cttaaagaac ttagggccat
    11101 ttcttcataa ccggtagcat gtaccatctg gcgtgccagt tcttttaatg tttctacttt
    11161 acgttcgcgt actggtcgat aaataatccc agcctggcaa aaacggcagc cgcgtgtaca
    11221 gccgcgaaac agctccaaga ctaaacggtc atggacaata cctccgtagg gcacgataaa
    11281 ctccgtgggg taaggagctt gatcgagatt agccactacg gcgcgttgta ctctagtggg
    11341 gagatcctct gtaatgggca cagtgcccaa atattctcca gaagtgctgc gtttttcctg
    11401 ataaagggac ggcacataaa ctccagtaat cattgctaag aggcgtaact tttcactgcg
    11461 gggtgctgct ttattttctg tcagcagcgt caagatttgc ggcagtgctt cttctgcatc
    11521 accgaggaga aaaaaatcaa agaaatctgc taaaggctct ggattaaagg cccctgggcc
    11581 accacctacc actagggggg cctgctctcc tctgtctgca gcagtgagcg gaatatttgc
    11641 cagggttaac atctgcagta tcgtagcata ggaaagctca tattgcagag taaagccgac
    11701 cagatcaaaa tccgcaagcg gcgtaactgt ttccaatgaa caaaggggaa atgatgcttc
    11761 ccgtaagagt tcctctgcat caggagccgg cataaagacg cgttctgcca gccaatgctc
    11821 ctcttgatta ataatatgat aaagtatctt tagtcccaga tgcgacatac ctatttcata
    11881 aagatctggg aaagccaaga ccacacgcat tgctgcttgt tgatgatctt tcacaatagc
    11941 attcacttcg ttaccgagat aacgtgtcgg cttttctgta cgtggcaaaa tatgctgcca
    12001 aaagtactgt tgatagtctt ttttcatgtt tcctccacca atagtgagat agtcaaactt
    12061 taaaaagtat actttgtagt atattttccc cctttctata attttacatg aatgtagtat
    12121 ttccctgctt agacttacca aataaaaacc gccctgccta tttatgccgc agggcggatt
    12181 caatggtggt ataaagtccc tctcgtacaa tcgtctccac aacttcatcc tccatcactt
    12241 cacccaaaac ttggccctcc ctcgtaacca ccagaacacg atgaaatttt ttcatagcaa
    12301 aacgtcgtag aatgtctttc attaatgtat cacgcaatga aagtagactg actaagggca
    12361 tgacaccctg ttcgtagaga acccttttct ttctccccaa accgcgaata aaagcataca
    12421 tagctgctgt tttttccttt tccgaggcaa aatacaaaaa agtggctgcg gctaagagtg
    12481 taacatgaaa atcacctaaa taaaagagat agagcgtcag ggcaaaaagc agcagcgcca
    12541 gcagttgact aatcctaatt gccagttctg ttgcctgttg aaagccaagt atcccacaga
    12601 ggcgagctcg caaaatccgg ccaccatcga gaggcaaggc aggtaaaaga ttaaaaaatc
    12661 ctaaagttaa attgctgcgg ataaagaata gcagtaattc attttgctgc cactcaggaa
    12721 cattagcata aagcagcagg gccagggcga caagtgcaaa attaaagagg gggcccgcta
    12781 aagaaaccac actttccact tgtgggtcca gctctaaaac atcctctatc ttggcaacgc
    12841 cgccaaaggg aaacaattct acctcgctca cctctaaccc ttgggctcgg gctgtgaaca
    12901 tatgtaccag ctcatgtgct aaaaccaggc caaataaaat caaggtttca cctaaatagc
    12961 caaaaagggc caacaaaaaa agcagtaaga taaacaccca atggaagcta aaagtaatac
    13021 cgccaagccg gaaaagtttc aagaataggc ctctccttta gttggtttgc ttaagatacg
    13081 gttcgggatc tacaggttgt ttttcttgcc agacttcaaa atgaagatgg ctttctcctt
    13141 cagcagcagc gattttagca atctcagcac cttgggcgac ttggtcccct gcgataacta
    13201 aagaacctgt gacacgtcca taaattgtta ctgtgtcatc gacatgttga agataaatag
    13261 tctcccccaa aactggatga gcaggatcca ccaccgttac agtcccagct aaggcggcat
    13321 aaactggtga acccggttca gcactgacat caataccata atgcatttcc atctcattac
    13381 taccagtgat gatacgtgca ccataaggac tggacagttc gccgttaact ggagcaacca
    13441 taccgtcaag agtagtaacc gtttcattag ctaaaggatc ttcggttgtg gactgctggc
    13501 gccagctaaa atcacgcagt gatgccaaca ccggttgggc agcagcaata atgtctgtcg
    13561 ggttagtttg cttgacagtc aattgatagg cccagtccac cacgcgatta gttaagggtg
    13621 tattaaataa actaacggca cctaaacaaa tgatcacaat aatagctgct acagttttct
    13681 gaaattgatt ttctcgccag tgtttagtca gcagatacgg taagtcgaga gcttgcggct
    13741 gtttataatc ctgtgtgtat tcattgtaat acgatgtgct gctttttgcc cggcgttgtg
    13801 ctagtcgttc tcgcaggccg aattgaggag cctgccatct acgattatct ttcactatgt
    13861 tcacccctta gaggtttgtc cttacccatg tatattaact tgtccgcaaa gatatacatg
    13921 attttaggaa aaatcagcaa caagaataat ccacccgcgt cttcacgggt ggattattct
    13981 ttacagctag ggttttgtta gttatttaga acattaattt ttgtcgtctc ataccaatat
    14041 tcaggacaac gccaatagcc atcatgttca ttagcaggga actaccacca tatgacatga
    14101 agggcagcgg aattcctgtt actggcataa tgccgatcgt catgccgata tttactaaaa
    14161 tatgaaaagt catcatgatg gctacaccaa tacacaccag catgccgaaa gtatctttag
    14221 cctgagcagc caggcgaata atgcgcagga ttaaaaaagc ataaagcaga accagcagca
    14281 cactacccag cagacctgtc gcttctgcat acgccgaaaa aacaaagtca gtatgctgct
    14341 ctaacaaaaa atttaaagcg ttttgagtgc cgggagcgaa taaaccttta cccactaaac
    14401 cacctgaacc cacagcaatt aacgactgga taatttgata gccatcattt aaggggtcga
    14461 ggtagggatt ggtaaaaata atgaggcgca ttttttgata atctttaagc gtaaaccaca
    14521 gtgctggcag tcctaccgct aaaccaatgc cggcataggt cattaaatgc tgaacttttg
    14581 ccccggccat atagagcatg ccaaaagaaa tggcagtaaa aaccagcgag gtgcccaagt
    14641 ccggctgcat aaagatcaga cccataggga gagcaacatg aaaaaaagca ggcagcaggc
    14701 tagccatgcc ctcaaaatct ccctcttgag cagataacaa gcgcgccata gagataataa
    14761 tgcctagttt cgcaaactcc gacggctgca gctcaaaaaa acctagatca atccagcgct
    14821 gggccccgcc accatcggag cccacaaata gcacagcaat cagcaaggcg aggacagtta
    14881 gataaatgta aggcgcaaag cgatggagat aggtatagtc gatacttaaa ataaaaagca
    14941 tggccacaaa acctacagca aagcggataa gttgtctttt ggcaaaataa agtggatcac
    15001 ccgtaatatt gatcgttgta ctagtgataa taagtaaact gattgctact atggcacaaa
    15061 cagtaagtaa aagcaaccag tcgaaattac gcaacagtct gcggtcaaac acattcccac
    15121 ctctgatctt tgttacataa ggttggataa aaaaaataag tgcaggaatt cctgcactta
    15181 tattatacgg taagttaacc agcacgtaaa gcggcaacaa cacccagcta catatttcga
    15241 cggatagatt tgatgcacaa gttagcctct aaaacggctg ttccttcgct atggtgcata
    15301 ttaatctgcg tttgattttc atcaatttcc atatatttcg atataacttg gatcatttct
    15361 tctttgattt tatcgaggaa ttcaggagaa acactagctc ggtcgtggac aagcactaga
    15421 cgtaagcgct cttttgccac atctttacta tcctgcctac ccattagggc attaagcata
    15481 gctctcaggt tcatagtcca cttcctttct acccaccaaa gccgatcagc ctttttagtc
    15541 ggctaaagaa tgagtggttc tgattcaaat ctaaaaatgt tacagcctca ccaagaatcc
    15601 tttgtactac attacggtaa gcttgtcccg ctaaagaatt actgtcagca actactggct
    15661 cgccccgatt tgtagaaaca attattcttt catcatccgg gattacacca actagctcaa
    15721 tcgctaatat ttctaagata tcatcgatat tcatcatgtc accgcgttta accatttccg
    15781 gtcgcaggcg attaatgagc agtcgtgatt cttctaactc tgccgcttcg agaagaccaa
    15841 taattcgatc tgcatcacgc acggcggata cttctggtgt agtgacgata atcgcctgat
    15901 ctgcaccagc aatggcattg cggaaacctt gttcaatgcc tgccgggcaa tccactaata
    15961 tgtaatcata ttcttctttt aactcgtcac ataatttttt catctgctcg gggctgacag
    16021 catttttatc ccgtgtctgt gccgctggca gaagaaaaag attctcataa cgcttatcac
    16081 gaattaaggc ttgcttggtg cggcagttac cttctaccac atctaccaag tcataaacaa
    16141 tcctattttc caaacccata accacatcta ggttccgtaa cccgatatcg gcatcaatca
    16201 agaccacttt tttgttttgc attgccaaac caacgcccag attagctgta gttgtggttt
    16261 ttcctactcc gcctttaccg gaggtaacga caatcacttc tcccattttt tcaactcctt
    16321 tttagatgat aacctttcta ggaacccagt ttggttgtaa tcgctgcaat catcacgccc
    16381 agatcactaa catacgcata ttccggctgc tccggcagtt cagagtcatc tggtgcccga
    16441 gagataatat cagcaatgcg cagctgagtg ggggccagac gcaaggcgac aacagaagcg
    16501 ctgcggtcgc cgtcgacgcc ggcgtgggca atcccccgca atgtaccaaa cacatagata
    16561 tccccatcag caacaatttc tgcaccaggg ttgacatctc ccagtactat tacatgacca
    16621 gggaagcgaa tatattgtcc tgaacgcagt gttcgcttga taaccaaagc acgaccttct
    16681 ttaaatccat ccagtcgcaa atcatctgtt tcaccctgtc tgcgagccgc tgcaatggcc
    16741 ggcggacgat cactagccga atttatcgct attaagtgaa ggcgactgtt ttgccttact
    16801 acatcaatta gtgcctctgt ttcttcttct gttaattccc tttgcccaac atcaatacgt
    16861 acaggtgcct caccgaaaaa agcagctctt tgtttgaggc gttccgccaa gtcagacata
    16921 atatcgctaa atgctgcatg atccagacaa tagatcgtta cgccgtcttt attcccttta
    16981 aattccaccg ttatgttact cataatctcc tccactgcac aacatatcac accttatttt
    17041 tcatcaatca gcgccaaatt cctgcaaacc tctgccaaaa accacgggta gagcccgtgg
    17101 cttttaatcc atttctgcca aggcaaagta ttcttcgaag atcagtttgg ccacaggtac
    17161 gccaccatcg ccacccaagc caccgttttc cacaattaca gctagagcca cttccgggtt
    17221 atctgcaggt gcataaccca caaataagct atgtgagatc ttccctgcac taacttgagc
    17281 tgaaccagtt ttgcccgcta ccttgacagg aacagtacgt aagacagcac cggtgccgcc
    17341 aggttcagtc acagctttca tgcctgtacg ggcaagttcc cagtttttct cactgtaagg
    17401 taaagtattc agcacctcgg gtttcgcttc ataaaccact ttacctttac cgttaagcgc
    17461 tttttctact aaatagggac ggtaatgggt gccatggtta gctaaaattg ccgcataatt
    17521 tgccaactgc aaaggtgtga tcgcatgacc ttgaccaatg gcagctgtga gcacatcacc
    17581 gggcataaaa ttactgcgcc gctccttgga gtcaatcacg ccagccacct caccacgaat
    17641 gtccgtcaga ccagtcactt tgccaaaacc aaaatcgtgg gcataggctg ctaaacgctc
    17701 aatacccaaa cgataaccca tctcatagaa gaaaatatta caggattttt gcagtgcgcc
    17761 aatgatattt aagtgcccgt gagcagtact attaaaacag cggcggcggt cgctaccaaa
    17821 aaaagattta acacctacac aggtgacacg tgacttttct gtaatcacac cttcttccaa
    17881 ggccgccagt gttcccacaa gtttaaaggt agagcctact ggataagtgc cttgaatggc
    17941 tttgttaatc tctggtttat tggggtccgc ctgaatctcc ttatagtcat taaagaatgt
    18001 attgggatta aacccaggga agctaaccat agccaaaatt ccgccagtgt tggggtcaag
    18061 ggctaccaca gcacctctgc tggcttgagt gttgcccttt ttctgtaaat cagcaatccc
    18121 ctgcgccagg gaccgtgccg caatctcctg cagtcgtgta tctaaagtca gctcgaggtc
    18181 attaccgcca actggttcag ttctttctag gacacgcaca cggttaccaa agcgatctgt
    18241 ttcaatccgt aaaataccat cctgcccgtg taagtattgc tcataagtgg attctaaacc
    18301 tgcttgacca ataatatcgg taaaacgata gccctcagct ttcatcgctt catattgata
    18361 gtcgcgaatg acgccaacat aacctaaaac gtgtgctgca agtgtctgta agggatattc
    18421 acggactggc tgtgtttcta taatcacgcc aggcaaatcc atggcatgct cttccaattt
    18481 agctacaatt tctgcggaaa catcattagc aattcggatc ggggcaaacc ttttgtactg
    18541 ctgctgcaaa attttctctt ggatttcctc tttttctatt tctagcgtct cacttaagta
    18601 gtcaataact tctgctgctt tttgtcgatc caaatccagt aaagatacag aaaaacccgg
    18661 tcgattagta gcgagactga taccattacg gtcacgtatt tcgccgcgtg tcgccgtaat
    18721 gggcagatca cgcaggcggt tattttcggc ctgccctaga tagtgatcgt atttgtatat
    18781 ttgcagccaa gctaaacgtg ctgttaaaag cagagcaaca aagataaccg caaaagtaaa
    18841 catgcgcagt cttttttgta gtgatcttgt cataacttac tccactcctc tgcttcgctt
    18901 tctgcaaaga gcagattgcg cttttgagca cgaaaaagaa aggggtagat aatccccata
    18961 attaaaaaat gcatcgctat gtgcggcaaa gttacctgct tcaggtaagt aagaagaggc
    19021 agcgggaatt gtatctgata aagttttagt aaaataaaac tgatcaaatc actgataata
    19081 gtaaaaatag gcattaacac catcgcggac aaggttagtt ctttattaat atcgctggag
    19141 agcaagcccg cggttgtacc taccagcatt ttactaagcg caaaaaagcc cagtggtgaa
    19201 ccaaataaga tatcttgagc tagaccggca cacaatccta cgatagcacc acgacgggca
    19261 tccgctaaaa gtgctaaagc aactgtcacc acaagtaaaa tgtcaggctg tacgccactg
    19321 ggcccagcta gtgccagcac agagccttgt agcaccaggc tactgataaa cagcacaaaa
    19381 attaagagcg cagccattaa agattctcct cttcctctgg gatcaccggc aaatcggact
    19441 gaggaataac tacaaaaact tcctccaaac gattaaaatt agccgctggc tgcagttgag
    19501 catattgtgt caaacccatc tcatcctctg ccgtctccag gacataaccg atcagcaaac
    19561 ctttaggaaa aacaccaccg agccctgagg aaataatggt atcccccgct tgaatattgg
    19621 cttcacgggg taaatctttc atgcgtaaat acgcggtatt attcggatca tactctacga
    19681 caccaacttc gcctggttct cgtgaacgct gtactagagc actcacacga cggcttgcat
    19741 tcgtcaaaag ttgtacctgg gctgaattag gggcagctga aaggatgttg ccgattaatc
    19801 cttctgctgt gaccacggcc atatttggtt cgatgccatc ttttgtcccc tggttaattg
    19861 taatcgtctt aaaccagttg ctgggatcgc gtgctattac ttctgcagta atcagttcat
    19921 attcactagc ttcacgatat tccagcatgc tgcgaagacg atcgttttcc tgccgcagtt
    19981 cagctatttg tgtttgcaag gaaaccgcct cggttaattg ctgccgcagg ttttcatttt
    20041 cctgacgtaa aagtcgataa tttctggcag ttgaaaaaac actctcaaca gaacgggtta
    20101 aacgttgaaa gaccccttgt acaggggcca caacatactg cagcatttct tccggccatg
    20161 tagcaccttg ccggttagca gtcacaccag taagtaacaa cagaacagat agtacgctga
    20221 ccagagaaat tatttttaga ttattatttt tacggttatt tttctgaaca tgcacgggca
    20281 tcacctttca catctgcccc gccaagccta atgcatgcga cgcggggcaa gggctactct
    20341 gcgtaacaat tctatttcgc caagtgcttt tcctgccccc agcgctacac aatccacggg
    20401 gttctctgca atatagacag gcatgcccgt ttctctggca attagcttgt ccagaccttg
    20461 taagagagct ccaccgccag tcatgacgat gcctttatcc atgatatcag ccgccaattc
    20521 aggtggagtt ttttctaaag tgattttaat ggcttctaaa atactagcaa ccggatctgc
    20581 taaagctgaa acaatttctt ccgccgaaat ttcctgcgtt ttcggcagcc cactgactaa
    20641 atcacgcccg cgaatatcca ttttctttgg ttctacatcc tcacggtacg cagtgccaat
    20701 tttaatctta acttcttccg cagtccgttc accaatcatc aaattatagt ttttcttaat
    20761 gtactgcaca atggactcgt ccatttcatc gccgccaaca cgaatagaac ggctagttac
    20821 aatcccccct aaggagataa tcgccacttc agtagtgccg ccgccaatat cgacaatcat
    20881 gctgccggtg ggttcttcta ccggcagacc tgcaccgata gctgctgcca tgggttcttc
    20941 aattaaaaat gcttctttgg caccagcttg cttggtggca tcaattacag ctcgtttttc
    21001 tacttctgtt acaccggaag gtacgcaaac cacaacctgt gccttaaaca tggtagtacg
    21061 tttataagct ttattgataa aatgacgcag catagtttgc gttacgtcaa aatccgcaat
    21121 aaccccatct ttcatcgggc ggatagccac gatgttaccg ggtgtacgac cgatcatccg
    21181 tttagcttct tctcctacct ccagcagcga accggtatcc cgtcgaatag caaccacgga
    21241 tggttcacga aggacgattc ccttaccctt tacaaaaact atcgtatttg cagttcccaa
    21301 atcaatacca atatcacggg aaaaatattt cgtcacaaag cgcattaaag cgtctccctt
    21361 cctcataaaa tccactaata aatattatat cagttttggc gttgtacaca atacacaagt
    21421 ttcgctttac aaaaattggt tagcgggtga ttaggccttt ttcccgaaaa cttacaaagc
    21481 gaccgtcgcc aatcactaga tgatcgagca cttcgatccc cattaggcat ccggcttccg
    21541 taagtctttt tgtcacatct acgtcttcac ggctaggtgt agggtctcca ctaggatgat
    21601 tatgtattaa gatcacgctg gcacaagcct tgcgaatagc cggacgaaaa acttcacgcg
    21661 gatgtactaa tgatgagtta agactaccta tggaaatgtc ctccatgccc agcactttat
    21721 tcttggtgtc cagcaagagt acacggaaat gttcccgcag tttatgccgc agctcttcca
    21781 ttaccagccc cgcggcatca ctcggtgacg agacagtgac ggattcataa cgagtacgat
    21841 tacttaaacg ggtcgccagt tcggctgcgg ccagtaaggt cactgctttg gctggaccca
    21901 aacccttgat ggattgcagc tcctccctac tgctgcgcgg cagctctgtt attcccccca
    21961 cctttaagag tactttttcc gccaattgta cagccgtttc ttgtgcagta ccactcctta
    22021 acaaaatagc caacaattct gcatccgaga gggcagcgac gccattcagc agcgctctct
    22081 ctctaggcct ttcacttggt ggcagctcct tgatggtcag gcgcgctgtt gtcatactct
    22141 ccagctcctt cccccaattt aatgcctgca gccattaaaa gctgccataa agcagctaac
    22201 ggcaggccca ccacattaaa ataacaacct tcaatcttct cgataaagac agcggctttg
    22261 ccctgaatac cataaccgcc ggctttatca tagggttctt cagtgttcac ataccaatca
    22321 atctctgctt ctgttaactt acgcagatag acagcggtac tttccacatg cgtccggacc
    22381 aaacctgctg gtttactgat gacagctacc cccgtcagca catgatgcag cttaccggat
    22441 agccgtcgca gcatttctgc cgctgcttgt ttggtagcag gtttgcctaa aagctcaccg
    22501 ttgatattca cgatggtatc cgccgcaata ataacgccct catcgacttt ttcggccaca
    22561 gcctgtccct tataaagcgc tagctgtgcc gccaattgtg ctggctctgc ctcattaatc
    22621 gtagattcat ctacattgct cacctgcact gtaaacggca gaccgatttg acgcaacagc
    22681 tccgcacgtc ggggtgaagc agaagcaagg ataaatttag tcatcaacac tcacctaatt
    22741 acgtttaatt tctgcaatca cattagctaa agccttttga ctgtacatga cagccagacc
    22801 tgtgaagaaa ccggtcggca gggctaacac caacggtacc ggcaggtaaa gagcaataag
    22861 tctaggagta ctggttaaga tactggctac caccacttgc gttgtattat gagctaccgc
    22921 acccaggata ctaacggcaa tcattgatat tttgcctctg cgccagaagt aaataccaac
    22981 agccatgatc agcgtagaaa ttatggcacc ggcaaaactc aaataaaaac caaccgataa
    23041 aagcgtacct gcaaacatgc ttcccaacag cgtacgggca acagaaacaa acattgctgc
    23101 ccctaaaccg tagaggacaa aagttaacaa agtgatcaca ttagccagac ccagcttggc
    23161 accaggtatg ggcataggca gcgcagattc cacagtatga atcactaggg caaaggttat
    23221 cagcattgct aaatgcgtaa gtttcttggt cttcatgttc ttattttcac ctttcatttt
    23281 gcctaaaaat caaattttgt ctgccatcag tttaccatgg aaagagcacc accgcaacaa
    23341 atgcagtaat aaaaatggta gcgctttcag tttaccactg caagcttttt atactgctaa
    23401 tcgcagcagt ataaaaaaac agctaagccg cttttagctg cttagctgtt agtgttagtg
    23461 ttagtgagta tgacttgtat aatcgcgggt tttatcatgt gctctgttct gacgtaatgg
    23521 tgctaccatg cgtcttagca tcatgcgtac cggttgataa agcagatagt cgatgctgag
    23581 ccagcacggg aacttaatag agaaaaagtg aaaacgatcc attgcccaga acaaaataac
    23641 ggccagaccg acaatgacaa tcatgccctg gatatcatgc cagttccaaa cattaatagc
    23701 atagagatgc tctacagcat gaggatcaaa ggtaaagcca gagagggcag gaataattaa
    23761 tttttgcagc agataatgtg ggaataagcc aatggcgaca ataattgttg ctaacgttcc
    23821 tagcgctgtg tatacaggcc aggtaaaact atatcgtttt ttatcatact tctcaggtac
    23881 ctctcccaag aaaaggccgc gaaagaattt tgtaaagtag catagcgtca tggcactagt
    23941 aagaacaaaa atcttttctg ctatcgccag aggcactgaa tgactgtgcg ccgctgcttc
    24001 caccaaagca tgatggagta atgttttact aacataaccg ttaaaacccg gaataccgcc
    24061 aatgccagcg aaggcaatga ggaaaataac tgtaattaaa ggcacctttt ttatcatgcc
    24121 acgtactaca gtaatgtcta gttcatgagt aatcatgtaa atagcaccga ctactaaaaa
    24181 cagacaggcc ttaaagaagg catggttgac aacatgatac aaagctccgg cgaaaccgat
    24241 tgctccttct gcacccaggt aagacgccgc acctataccc ataataatat agcccatttg
    24301 cgagatggag ctataggcaa gaagtttttt ggcatgagtc gacagcaggg ccatcaaggc
    24361 tcctaaaaac atggaaacaa tgccaaccca aatcaacaga tagccaaaat tcatggctgg
    24421 taacaaggtc gccaagtcat gcattactgc cgtgggcgta aagatcatgt ctactacccg
    24481 tagaataccg taaacgccag tttttataaa caaggcacat aacagggcac tgacaggggc
    24541 aggtgctacc ggatctactt taggcagcca aatatgtgtt ggcgtcatcc cagccttaat
    24601 gccaaagccg atggtgaaat aaattaaaag ccaataagag ttaagaccac tttgtgctat
    24661 ctggtcatag agaggccgaa tttccaaagt gccaaccccg gtgtacagtt gatagatacc
    24721 aaggagcagg aaaaagctgc caataatccc caggtagagc atcccattgc ccgcactcat
    24781 agcatctgct gtatgctcat gcgtgaccaa gatatgggat aaaaatgtaa ccgcttcgta
    24841 aaaaataatt aatgtgatca gatcaccggc caggacgaaa ccaagacagc tgctcagggt
    24901 agctaataag cacgtaaaaa agcgattttg atcatgttca tgggccatat aagccgtggc
    24961 atgcagggta gttaaacacc agataataga aataagtgcc gcaaagacaa agctaaagaa
    25021 atcaacacgt agaaaaatat taaattgttc aagcctgagt aagataaatt ccactgcacc
    25081 cttttgcagc aatggataca gggccataac aacagtaatg gtggccagtg tcacaagtac
    25141 tgtacacgca ttccgtagtt tatcactacg ctgaccgagc cagtaagtta acagtgctcc
    25201 cactaggggc accataattg cagctaatgg tagcagtgaa ctaatagtct ctgtagttgt
    25261 attcatctca tgtgcagccg ctggcaacat ttttaaattc ctcccccgca taaagaaatc
    25321 agcagctaaa tacaaatctc ttctgtttct tcctaggtaa taataaatct tgctgactat
    25381 ccatcatgca ctaccaattt ccgatacaga taaaaggatt atctatacca ttctatgcta
    25441 gtgaatgtgc taactttcac taatcgccac tattcagcaa aaatgatctt aaagcatgct
    25501 tgcacttgca gttcttcact gcaagccttg taccggcttc attaactact tgctaaatac
    25561 aagtgattta cttgcctaac cagtacaagc aggggctgag ggtgcggaag aaagacttcc
    25621 gcaccttcag cctctaaaca atcgtatgct ctggacatac ttccacacaa ataccacagt
    25681 tgttacaaag tgaactatcg ataacagcca cattgttttc catataaatg gcgttttctg
    25741 ggcacttctt aacacaaagt ccacaggcaa tacaacctcg ctcacaaaca gaccgtacgt
    25801 actttccttt atcccgtgac ttgcatagga catgaggttg cgctgccgtg cttaccatct
    25861 caataacgtg gcgtgggcat tcagctacgc agttaccaca gctaatgcac aattcaacat
    25921 ctatcaatgg caaaccattt tcccccatgg taatggcatc aacgggacag accttaacac
    25981 aattgcctaa ccccacgcag ccataactac aagcgctagg cccaccattt agagcaatgg
    26041 cagctttaca gtcagctata ccatgataga gatgttttgc tttgaccgta ttacagtcgc
    26101 cttgacattt tagcagtgct acacagcgtt cctgtgcttc cggggcttct gcacctagaa
    26161 tttccgcaat acaagctgcc accttactac caccgggtgt gcagccggtg atggaggcac
    26221 gaccagccac tacttcttca gcaaattgtg cacatccggc acagccacag gcaccacaat
    26281 tagcacctgc cagtacttct aatacttgtt ctattttagg atcaatctca atggcaaact
    26341 tttgtgaggc aaaagctaac atgctaccaa agattaaccc taaaccacct aggctaacta
    26401 ctgctggtaa gatcatttac tacgcctcct acatcatgcc ttgaaagccc ataaaggcta
    26461 tggaaagaat tcctgcaaca attaaggcaa ttgggacgcc acgcatggct ttggggatga
    26521 aaaccagctc taaacgctca cggataccgg ccataatcac gagggccagg gtaaagccaa
    26581 gggctcccgc aataccaaag actacggatt ctaataagtt gtactccaat tgtacattta
    26641 agagggctac gcccaagacg gcacagttgg tggtaatcag gggcaggaaa atacccagcg
    26701 cctgatacaa ggcaggactt actttgcgga ttactaactc tacaaactgc accaaagctg
    26761 caattactag aatgtacaca atcgtacgca agtactgcaa actatatgcg gctaagacat
    26821 agtgttccag cagccaggtg accaaggaag acatgcccat gacaaaggta actgccatgc
    26881 ccatgccaag cgacgtttct actttcttgg aaacaccgag gaacgggcag atgccgagaa
    26941 acctgctcaa cacaaagttg ttaataaaaa tcgccccaaa gacaatggtt aagatactcg
    27001 tcataatcta cccctcctta ttccaaacga aattttttag ctaaaatatt cattaaggcc
    27061 atcagtaagc caagagcaat aaacgcacct ggcggtgaag caaatacttt aaagggctga
    27121 taagcagcac ctaaaatatt agcaccaaac agtgtaccgc tggccaccag ttcccggata
    27181 gacgcaagaa tagttaaaga gaaggtaaag cccaagccca tacccaaacc atcggctaca
    27241 gagctgacta ccgggttttt ggaggcaaaa gcttccgccc gccctaaaat aatgcagttc
    27301 accacaatta aggggataaa aatgcctaac tgggagtgaa gctctggttg ataagcatta
    27361 agcagcatat ccaccatcgt cacaaaggtg gcaatgatga tgatataggc cgggatacgt
    27421 attttatcgg ggattaactt ccgtaacagc gaaatcataa agttggaagc tactaacaca
    27481 gcagcagtgg aaagacccat ccccagacca ttaacagcta gtgtcgttac cgctagtgta
    27541 gggcacatac cgagcactaa acggaaggtg gggttttctc gaaacaagcc tttagttagt
    27601 tctttcatca cactcacttc tcttcacccc cattattgct gcagtgcatc aagcactgca
    27661 tccttaatac cgttactgct gaaggtagca
//
LOCUS       DASRMS010000062         2316 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_215774, whole genome shotgun sequence.
ACCESSION   DASRMS010000062 DASRMS010000000
VERSION     DASRMS010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_215774"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /locus_tag="VFC74_02665"
     CDS             complement(<1..285)
                     /locus_tag="VFC74_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007861958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZK24272.1"
                     /translation="MKAQMQKGFTLIELMIVVAIIGILAAIALPAYRDYTVRADGGAA
                     LQQAAGYKTCVAEEYQTGSAKPQDCDPGQVNAALTASSLSTDTNHGKVHVS"
     gene            596..1063
                     /locus_tag="VFC74_02670"
     CDS             596..1063
                     /locus_tag="VFC74_02670"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24273.1"
                     /translation="MNLAISLDTAALITDVSKRTLWRRLDDGQISRQENDSRGRAMLT
                     LADLVPMLCVPIEPEDYELLADADAGNADAQNDLAQLFLDANRSDIATHWIQLAVDQE
                     HPDAIHNLAMLYIKGIGVGRDRTKGLMWLAKAATLGHPIAKQQIATLHIELRD"
     gene            1919..>2316
                     /locus_tag="VFC74_02675"
     CDS             1919..>2316
                     /locus_tag="VFC74_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24274.1"
                     /translation="MKTRNFNLGSRDMTRAAATALNADRAAGKISYLTVAATLQRFDK
                     FKEFAKEAGAGRFEKIDRALVQQYAQNLRESAYSASYQQNLLSAVNSVMNRVSEHGGR
                     NWETVSPRAEGLAQRSFIRTEQTASREQSSV"
BASE COUNT          647 a          594 c          514 g          561 t
ORIGIN      
        1 actaacatgt accttcccgt ggttagtatc agtacttaga gaacttgctg ttagagcagc
       61 attaacctgg cctggatcgc aatcctgtgg cttggcactt ccagtttggt actcttcagc
      121 aacgcatgtt ttgtaaccag ctgcttgctg taaagcagca ccaccatcag cgcgaacggt
      181 ataatcacga taggcaggca gtgcgatcgc cgccaaaata ccgatgatcg caaccacgat
      241 cattaattca attagggtaa aacccttttg catttgtgct ttcattttta acactccatt
      301 ttgtgagata gccgttgggc taataaacac aaagcagctt tcatgccatc ttttttgaag
      361 aataatggct gaaacaggct taactcctct gattctgact gtttattaac ccctcttttt
      421 gattcgtact ttgtcttgat gtgacaaaac agcccctgtc acgagccgaa gtgtgccaaa
      481 acaaccctgt catgtgccaa ggtgcgccaa aataaacact gtcatgtgcc aaggtgtgcc
      541 aaaatagtaa tttgtcatgc ttcaaagtgc gccaaactca aagtaaggat caaaaatgaa
      601 cctcgcgata agcctagata ccgcagccct tatcactgat gtcagtaaaa gaacattgtg
      661 gcgcaggctg gatgatggac aaatctctcg tcaagagaat gattcccgtg gtcgcgcaat
      721 gctgacactg gccgatcttg tgcctatgtt atgcgtacca atagaaccag aggactatga
      781 gttgctagct gatgcggacg caggtaatgc cgacgcgcaa aacgacctag cgcagttgtt
      841 tctagatgca aatcggtcag atatagcgac tcactggata cagttagctg tagaccagga
      901 gcaccctgac gctattcata acctagccat gctgtacatc aagggcatcg gtgtaggcag
      961 ggacagaaca aaaggtttaa tgtggctggc taaagcagcc actctcggac acccaattgc
     1021 caagcagcag attgccaccc tacacataga gcttcgtgac taacctgcaa cagtcacttg
     1081 aaataagctc agcctgtagt aatatcaagg ttcttgtcct caatgaggac gaagggtgcg
     1141 gtagctggtc gccaaagaaa aacagcaaat tagcaacacc cctaagcaac acccctagca
     1201 agatgtcgga tttcccaatc cacatcccca gtctcaggac tgtaattaga tgcatcaaag
     1261 accgagctac gccttttttt tgatgttcac actatctaaa aaaccacaaa aaaaacagtg
     1321 ttatgtacga cgtcgcgcgt atcgctctgt gattttttgt cggtcagaat ctcaccgccc
     1381 aggtgacaga gtttgttcca acgggacaat tctgctttca attaactata agtttccgtt
     1441 caggtttaat cggcagcaat tcatcgctgc cgagctgctt tcgaaatagc caattcaccg
     1501 gccgcccccg tctcttttga tacaaaagag acggggtctc aaaatcagca tcaaacctca
     1561 ctagaacggc acttacagcc catcatagac tgcatgaaaa tgcatgaaaa cgtaggtttt
     1621 catagcattc atgctcgtgc gttaacaccc gtccgctgac gctctttgca gctgatgctt
     1681 agcccccagg gagggcgcgg ggagcaccta ataattcaga aatacatcgg tatttgcttg
     1741 ggagcaacag gttgccaccg cctaaaaggt gcgcgtactt caactcaaca ctgcgccatt
     1801 catcccttca cttccaacaa ataccgttcg cgcatttcta tgcgttctgg ggcgcagtgt
     1861 gcctgttggc acagccccat ttttaccttt tccatcaatt tcactcgggg tttgttgcat
     1921 gaaaacacga aattttaacc taggtagtcg agacatgact cgtgctgctg ctacggcctt
     1981 aaatgccgat cgtgcagcag gaaaaatctc atacctaacc gttgcagcca cactgcaaag
     2041 attcgacaaa tttaaagaat tcgcaaagga agctggtgca ggtcgatttg aaaaaatcga
     2101 ccgcgctcta gtgcagcaat acgcccagaa cctgcgcgaa agcgcctaca gcgcctcata
     2161 tcagcaaaac ctactatcag ccgtgaacag cgtcatgaac cgcgtgagcg agcacggggg
     2221 gcgtaactgg gaaaccgtta gcccacgagc tgaggggctg gctcaacgca gttttattcg
     2281 cacagaacaa acggccagcc gtgaacagtc cagtgt
//
LOCUS       DASRMS010000063        53397 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_954007, whole genome shotgun sequence.
ACCESSION   DASRMS010000063 DASRMS010000000
VERSION     DASRMS010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 53397)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53397)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53397
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_954007"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(175..876)
                     /locus_tag="VFC74_02680"
     CDS             complement(175..876)
                     /locus_tag="VFC74_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HZK24275.1"
                     /translation="MDLLTPLLDLLFPRRCGFCRTLLNEAQPGVLLCRSCEQTILNPQ
                     SCCSYCGYPLAASEDGFCPLCSEHSYAFYASCALSLFQGELRKAILRFKYGGKRELAF
                     LFGQLMARQVRKCQWPALDSVVPIPLHPLKLRERGYDQALLLAQVLGKELDLPLQRIL
                     TRRQATVSQTTLSAAQRWQNVADVFVFHQQPSVPRRVLLVDDLLTTGATAHFAAEALH
                     QAGVKEVYLAVIAHS"
     gene            complement(891..3068)
                     /locus_tag="VFC74_02685"
     CDS             complement(891..3068)
                     /locus_tag="VFC74_02685"
                     /inference="COORDINATES: protein motif:HMM:TIGR01448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HZK24276.1"
                     /translation="MESLAGTLERITYYNEDTLFMVARLRSDDEQLHAIIGNMPRLSV
                     GERLVVQGEWTQHRDFGRQFKVARYEAEAPHNIKGIRNFLASGLIKGVGPSTAEKIVE
                     HFGLEALTIIAEEPERLCEIEGIGTKKSALIAGSLQERQEIIRVMTFLQSYGVSIGYA
                     ARIYRRYGDDTVARVSENPYRLASDVFGIGFKTADKIALEMGLAPNSPHRIRAAILYQ
                     LTQGQEQGHVYLPQTELSDTVVSALSTNTEPVTVEMVSEQSEGLAESKQIVLTELEGV
                     TVIYLTPLYQAECYVAARLLELANSQSSLASVEKAAWQEDDLTLAAAQQLAVQQAMDA
                     GLLVVTGGPGTGKTTTIRALIKLFHRNGLEVLLAAPTGRAAKRMTEATGLEAKTIHRL
                     LEYAQKESSGVGFAFQRDEDRPLQAHVLIIDEVSMVDLPLFYYLLKAVPEGCKLILVG
                     DMDQLPSVGPGNVLRDLITSEVVPVVRLTQIFRQSENSMIVSNAHQVNDGQLPLLSGS
                     EDFFFIQESDPEIILKKVLDLCSSRLPQYKNRDPIADIQVLSPMRRNRLGVDQLNVQL
                     QERLNPPAPHKPELTFGRTTFRLGDKVMQIRNNYQKEVFNGDVGRITYLNSDDGEVMV
                     LYPDVGGARDVLYDQAELEELVLSYATSVHKSQGSEYPIVVLPVVTQHYILLQKNLLY
                     TAITRAKELVVLIGTKKALAIAVRNNKVATRYSGLATRLVNYR"
     gene            complement(3274..3498)
                     /locus_tag="VFC74_02690"
     CDS             complement(3274..3498)
                     /locus_tag="VFC74_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HZK24277.1"
                     /translation="MDTTLQLIFRNEDGRLFTISIPNPRADLTEAEITTVMDLVLSKN
                     LFQSTGGALVQKVRARLVSRDSEDLASYEQ"
     gene            complement(3530..3754)
                     /locus_tag="VFC74_02695"
     CDS             complement(3530..3754)
                     /locus_tag="VFC74_02695"
                     /inference="COORDINATES: protein motif:HMM:NF019487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1659 domain-containing protein"
                     /protein_id="HZK24278.1"
                     /translation="MAVVLEPSYSRCQVRLQSGVDDEGNPVVVSRTYGRVLPAVTNDD
                     LHEVFTALMSLQGFSIYALRRLEDGELIAE"
     gene            complement(4146..5339)
                     /gene="metK"
                     /locus_tag="VFC74_02700"
     CDS             complement(4146..5339)
                     /gene="metK"
                     /locus_tag="VFC74_02700"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HZK24279.1"
                     /translation="MGKNYLFTSESVTEGHPDKVSDQISDAILDSILTHDPLARVAAE
                     TSVTTGLVLVAGEITTNCYVDISKIVRETVKEIGYTRAKYGFDGDTCAVLTSIDEQSS
                     DIAQGVNQSFESKESTNQDLEFEITGAGDQGMMFGYACNETPELMPLPIAMAHLLARR
                     LAEVRKQKLLPYLRPDGKTQVTVQYLAGKPVGIKTVVVAAQHREDISLTRIRNDVIEQ
                     VIREVIPAQYLTPQTSFYVNPTGRFVVGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDPTKVDRSGAYAARYVAKNVVAAGLADKCEVQLAYAIGVAHPISLLVETFGTAKVA
                     EEKIEALVNQHFDLRPAAIIQKLQLRRPIYKQVAAYGHFGRLDLDLPWEQTDKAALLK
                     DEAGL"
     regulatory      complement(5524..5626)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(5770..6198)
                     /gene="fabZ"
                     /locus_tag="VFC74_02705"
     CDS             complement(5770..6198)
                     /gene="fabZ"
                     /locus_tag="VFC74_02705"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HZK24280.1"
                     /translation="MNQAWDVQSILPHRYPFLLVDRLLEVVPGERAKGIKNVTINEPF
                     FQGHFPGYPVMPGVLVVEAIAQVGAVAILSMPEFRGKIALFAGIDKLRFKQQVVPGDV
                     LELELEVIKLRGTIGKGKGTATVNGKVVASGELMFAITQA"
     gene            complement(6212..7804)
                     /locus_tag="VFC74_02710"
     CDS             complement(6212..7804)
                     /locus_tag="VFC74_02710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK24281.1"
                     /translation="MKLTFCGGAQTVTGSSFLLEAAGQKILLDCGMFQGNKHLRARNF
                     NDFPYNPAEIDFVILSHAHIDHSGLIPRLVKGGFRGHILATSATVDLVSIMLPDSAYI
                     QEMEAEWQNRKNLRAGRPLIDPLYTREDAEKALKRFKEVNYYQKFWLSDRIALTFYNS
                     GHILGSAITELTIVGTERTEKIVFTGDLGKAKQPIIKDPDILEEADYVVIESTYGTRE
                     HEIEDQKIEILQQVIQQTILAQGNVVIPSFAVGRTQELLYFLSRLMRDHQIPNLPIYI
                     DSPLAISATEIFTRHPECYDLQMRELLKSGQDPFNFPEVIFTKTAEESKLINKSPGGA
                     IIISASGMADAGRIKHHLKHNLWRPESTVLFVGYQAEGTLGRRIRDGARIVKIFGEDI
                     KVNARIVSIDGFSAHADRSELLRWLGRFPKKPQQVFLVHGEENVLPEYAALIERELNL
                     NTYIPEYLETVELTTEAVADKRPELSLRQTSAKTLQQLWQDFQRDYSKRLELAILEGD
                     AELELQQLATELAEMRALLENE"
     gene            complement(7888..8586)
                     /gene="sfsA"
                     /locus_tag="VFC74_02715"
     CDS             complement(7888..8586)
                     /gene="sfsA"
                     /locus_tag="VFC74_02715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000023 - maltose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HZK24282.1"
                     /translation="MTLHLPFKPTEAGFFVERLNRFVALVELNGETVAAHIATSGRLT
                     ELLVPGASVLLERNNSATRRTAFSVRAVWYQQTWVSIDAQLPNKLVKQVLLAKALPPF
                     EQCEFVRSEPVYSSGRFDFLLAEAGQAVYVEVKSVTLVIDKVALFPDAPTARGRRHLA
                     HLADLQREGCRCAVIFIIQRGDADYFAPNRRTDLQFGQSLRQAASAGVELYAYRCRVD
                     QAGMTLAERLPVLI"
     gene            complement(8707..9459)
                     /locus_tag="VFC74_02720"
     CDS             complement(8707..9459)
                     /locus_tag="VFC74_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK24283.1"
                     /translation="MKQIAIAGKGGVGKTTFTALMMRYLLEHKSGKPILAVDADPNAN
                     LNEALGLEVENTISQIIEDTKTPNAVPTGMSKEMYMEFKLQDALAETNFYDLLVMGNP
                     QRSGCYCYPNDLLRKHLEKLRSNYDYVLVDNEAGLEHLSRRIISAVDMLIVVSDAAAR
                     SIRSAARVREIAESVNLDIKEMYLIVTKVTGNEIEVLQPEIDKTGLQFLGTIPLDPKV
                     AEYDVKGQALMELPDDSAAVQAVNAILQKLTL"
     gene            complement(9481..10815)
                     /locus_tag="VFC74_02725"
     CDS             complement(9481..10815)
                     /locus_tag="VFC74_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZK24284.1"
                     /translation="MTNRLEVNVVRFVQLLRHMGLRIGSSEVIDALQALQEVELSERD
                     AVRLALQATLVKRPGEQAVFTQAFARFFASPEVRAELEQEYAAKLAQRQEMLAEASED
                     LQFRDVPLALSEEEKLVYAAIPPAARQKMLDYIQRTAEGKQVEADFRPVLESMVRGSL
                     TFWRKSLRNELGQLALPQSDDPELNALLASMEEQPQAAADSLLAEDMQNIAEQDLPRA
                     QLLLQKLARQLVTRIERRFRQGKRRQQLDVRKTIRHNMRFGGTMFKLRYRNHRRQKPQ
                     LLLLCDVSGSMARYASFVLQFIYSIHSVVSSIEGFVFAEDVERVTDYFQASTDFTQTM
                     VELMNRSQEWGRGTELATALQSLRANYPHLLSSQTYLLIVSDTKTMKVTQAVQELAEI
                     KRMVKDVVWLNTLPAPEWEKASSVEAFQQVVRMYPCNTLLALEQVMRQKIIA"
     gene            complement(10818..11702)
                     /locus_tag="VFC74_02730"
     CDS             complement(10818..11702)
                     /locus_tag="VFC74_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HZK24285.1"
                     /translation="MEITVANLRKAFQTDGYITDDDVLLTVYLALKLQKPLLIEGEPG
                     VGKTELAKVLARVFQTELIRLQCYEGLDENKALYEWNYQRQLLKIQLSKESTSATELE
                     EDIFSADFLLARPLLKAIQAEEKVVLLIDEVDKTDEEFEAFLFEVLSDFQVSVPELGT
                     LQAQQIPMVILTSNNERELSDGLKRRCLYLYLTYPTVEREVEIIRARLPEVKEKLAKQ
                     LARAVAQIRAQQEIRKKPSIAETLDWARALVTLGKDSLDEVTVKQTLSLLLKRKTDQD
                     AFTERLGAGRLAALSQEE"
     gene            complement(11779..12648)
                     /locus_tag="VFC74_02735"
     CDS             complement(11779..12648)
                     /locus_tag="VFC74_02735"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK24286.1"
                     /translation="MEKVDVAIIGCGPAGLSAAINVQARNRTLLLVGPRICSQALHKA
                     HQVNNYLGLYNISGEAMLQSFLKHAKAAGIEVSHHSISAVYPLGDSFQLQLRDDVVEA
                     KTVVITTGVSAGKRLPGEDNYLGRGISYCATCDAMFFRDKTIAVSSDSAEHEDEVKLL
                     TEVAAKVYFLPQYEVSMSLPANVELLAGQKIRQFTGSASLEEMELKDGRKIKVEGVFM
                     LKEQYPLTQLIPGLALEDKHIKVDLQMATNIPGVFAGGDVVGRPYQVAKAVGQGQIAA
                     LSAVAYLDHKNKA"
     gene            12886..13557
                     /locus_tag="VFC74_02740"
     CDS             12886..13557
                     /locus_tag="VFC74_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HZK24287.1"
                     /translation="MPTIDYLGHACFLVTGQEHTLLFDPYLTDNPQAAISAAEVKPDY
                     ILVSHGHFDHLGDAFAIARRTGATIITTLDLAKECEKQQLPTHAMNIGCKHNFAFGYV
                     RTTQALHHAGLVGGEACGFIVNLDGYTLYFTGDTSLFGDLKLYGIIEHPDLLLLPIGG
                     YYTMDIDDAVYAAKLVNAKTIIPFHYNTWPLIEADPAVFAAKVAKHTSSHCVILAPGE
                     SCDLS"
     gene            complement(13612..13752)
                     /locus_tag="VFC74_02745"
     CDS             complement(13612..13752)
                     /locus_tag="VFC74_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24288.1"
                     /translation="MSTYAYNLNEVRDGLLREYMLATGATRIELLTKIMDLDEEIEGM
                     DN"
     gene            14281..15309
                     /locus_tag="VFC74_02750"
     CDS             14281..15309
                     /locus_tag="VFC74_02750"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK24289.1"
                     /translation="MSKLTPKENYLRLGRGEMPESVVRFNMGMPTISGEMTTAMIGPS
                     ILMPGHLSPKGGKDNWGVTYVTNEETGFASIPEPNNFMLKDITKWHEVIKRPELPERI
                     DWEYMAKKDYEKSKINRTESAVMAGCGLMPFQQLMAFMGFTEGLCAMFEEPETVKELL
                     HFMADFYVPIIDKTLDYYQPDIFYLLDDSAAKLNPFISVEMYRDILKPIYVKLTNAAR
                     ERGIPVQFHNCGRCEDFIEDMIDFGVVYTDPAQTTNDLLAVKEKFAGKIVLCGGWDWK
                     MPDTWPIVDEEAVRQSVRDTIDKYAPGGGFAIGGGVLGKYNDPVTKQVNGWLQDEAAI
                     YGKDYYNR"
     gene            complement(15378..15452)
                     /locus_tag="VFC74_02755"
     tRNA            complement(15378..15452)
                     /locus_tag="VFC74_02755"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15418..15420),aa:Thr,seq:cgt)
     gene            complement(15516..16259)
                     /locus_tag="VFC74_02760"
     CDS             complement(15516..16259)
                     /locus_tag="VFC74_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HZK24290.1"
                     /translation="MDSSRRLYRHSMQRPGFRAFTCQVKETDLYVAVDEASYGPQLEK
                     FVERRVLYYRTSLEQLIAREPEFQTTLQPYLVAAEEPPLILAMTRAGNQVGVGPMAAV
                     AGAIAEYVGHDLLPRVKQVIVENGGDIYFHVTEEVTVGIYAGTSPLSNRFAVAIRPQG
                     QDFGVCTSSGTVGPSLSLGRADAAMIISPSTILADAVATAAGNRVQSAADLEQALSWA
                     CQIPGVSGVLLIKDDQLAVWGQVKIVGNN"
     gene            complement(16262..16678)
                     /locus_tag="VFC74_02765"
     CDS             complement(16262..16678)
                     /locus_tag="VFC74_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIL domain-containing protein"
                     /protein_id="HZK24291.1"
                     /translation="MKRKLVLRFPAAVVEQPFIYTLAKDYNVVANILKANINPRKEGN
                     MVVELTAEAEGFNTALDFLQARGVEVSPLEQQIVWLEDRCTQCGACTVICPTNALWMQ
                     RPEMTVSFCSEQCVVCERCLKACPARAVEALYLAGE"
     gene            complement(16702..17871)
                     /locus_tag="VFC74_02770"
     CDS             complement(16702..17871)
                     /locus_tag="VFC74_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine biosynthesis protein"
                     /protein_id="HZK24292.1"
                     /translation="MTQLRSIKEINAKIAKGQAVVVTAEEVLALVAEHGVEETARRVD
                     VVTTGTFGPMCSSGAVLNVGHATPRMRMTKVHLNGVNAYAGLAAVDVYLGATEIPETD
                     PANSSHPGEFNYGGAHVIEDLVAGKPVLLEASSYGTDCYPRQQLKKQITLAEMNEAYL
                     FNPRNCYQNYNVAVNLTERTIYTYMGILKPQMGNANYSSAGQLSPLLNDPYYRTLGIG
                     TKLFLGGGIGYVAWQGTQHSPNAPRSANGVPRAGAGTLALIGDLKQMNTRYLRGVSLL
                     GYGVSMQVGVGLPIPILDAETLSYAAVRDEDIMAQVVDYGEAYPQGVGGSLAEVSYAE
                     LKSGTIVVQGKEVPTAPMSSYPRAREIAQTLKQWITKGEFTLTEPVAALPTVEAE"
     regulatory      complement(17945..18043)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(18171..19397)
                     /locus_tag="VFC74_02775"
     CDS             complement(18171..19397)
                     /locus_tag="VFC74_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HZK24293.1"
                     /translation="MNFEQLYASRAQCMKTSAVREFFKLTEQPEVMSFAGGFPSADFF
                     PMEKVTEVMQELIAEEGKLALQYGATEGYGKLRQYVAEKMTREGSPCTAEQVIITNGS
                     QQGMDLVSKLFVNTGDVVLVEEPGYVGGLGAIFNYEADRVPIPLDDDGIRMDLLANRL
                     RRLKMEGRAPKFIYLVPNFQNPSGICMSLERRQAILALAREYNFLILEDNPYGELRYE
                     GEAIPSIKSMDTEGRVIYLGSFSKTFCPGIRVGWLVAEEDVVAKIVSAKGSTDLCSNS
                     LGQRLAYRFAASGYIDQHVASLIGCYRDRRDLMLSCMAEHFPKGVTWTQPQGGFFIWV
                     SFPGGLTARQVLLKAIERKVAFVDGAGFYVNDHGKQTARFSFSEASPEKIKQGIAILG
                     EIIAAELAACKKVRTA"
     misc_binding    complement(19482..19698)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(19847..20437)
                     /locus_tag="VFC74_02780"
     CDS             complement(19847..20437)
                     /locus_tag="VFC74_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HZK24294.1"
                     /translation="MSKTTSLVMGGVGGQGIVLASKILAQVIQDHHYQLKVSEIHGMA
                     QRGGSVLTFVRFGAAVHSPLIELGQADYVFALEKLEAWRLLPYLRTGGTMICSSQEIK
                     PMPVIMGRQQYPAQIIPAMQALAAAGQLGALHTFNALEAACDCGQPKAANVALLGVLA
                     RTLEFSLASFLAALAAVVPERFLEANQQAFLVGYQG"
     gene            complement(20430..22181)
                     /gene="iorA"
                     /locus_tag="VFC74_02785"
     CDS             complement(20430..22181)
                     /gene="iorA"
                     /locus_tag="VFC74_02785"
                     /EC_number="1.2.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043805 - indolepyruvate ferredoxin
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate ferredoxin oxidoreductase subunit
                     alpha"
                     /protein_id="HZK24295.1"
                     /translation="MKKLLSGNEAIARGAYEGGVHFAAAYPGTPSTEILENLATYQEI
                     DAQWSSNEKVALEIGIGAALAGSRVLVAMKHVGVNVAADPLMTFSYLGVNGGLVLVAA
                     DDPGMHSSQNEQDSRQYARFAKVPMFEPADSQEAKDFVREALRVSEEFDTPVLLRTST
                     RISHAQSLVELGERQEIAIKPYEKNVQKHVAMPANARPRHVAVEERLQRLRDYAEQTP
                     LNRLEWRQRKIGVICAGISYQQVQEALPEASVLKLGVCYPLPAQLIAAFAAGVEELYV
                     VEELDPFFEEQIKALGIAVQGKALFPLTGEIFPNLIAEKILGEPLTVPLAVAASREEP
                     APVRPPVLCPGCPHRSVFYTLRRQKLLVSGDIGCYTLGASPPLEAMDSCICMGASISA
                     GLGYEKGNPDLKGKVVSVIGDSTFFHSGMTGIVDVVYNRGTTLTLVLDNRITAMTGHQ
                     ENPGTGTTLQGQPTVALDIAAICRALGVQRVREVDAYDLAALDAVIREEIAAGEAAVV
                     IVRRACALLNKSVPEVYYIVEEKCTSCGACMKLGCPALVQEGKKITIDAALCVGCGVC
                     VQVCKFEAIRQAGEQDV"
     gene            complement(22178..23494)
                     /locus_tag="VFC74_02790"
     CDS             complement(22178..23494)
                     /locus_tag="VFC74_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK24296.1"
                     /translation="MIWGAKTETMSIEALRALQFTRLSSTVSNVYENVPFYRELFDNH
                     GVHPADLQSLDDLQKFPFTSKDALRDNYPFGLFARPMAEIVRVHASSGTTGKPIVAGY
                     TKNDLATWTDLVARIAVQAGVTSLDVAQICFSYGLFTGGFGLHYGLEKVGVTVVPASV
                     GNTEKQIMLIQDFGVTTLIATPSYALYLGEVAAEMGIDPRTLPLRIGMFGSEPWTDQM
                     REEIEERLNLRATDNYGLTEVMGPGVAGECAARNGLHISEDHFYVEVINPETGEVLPE
                     GSEGELVFTPLTKEGFPVIRYRTKDISVVTRECCACGRTTARMRRVTGRTDDMLIVNG
                     VNVFPSQIESVLMEIEEVAPHYLIIVDKKKRYLDELEVRVELNRDRFTGSFRDLEAVE
                     QKITSRLASVLTVQARVKLVEPKSLERTAGKAKRVIDKRQQQEVGA"
     gene            complement(23662..24093)
                     /locus_tag="VFC74_02795"
     CDS             complement(23662..24093)
                     /locus_tag="VFC74_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZK24297.1"
                     /translation="MNVKQISVFLENKSGRLADVTRSLGAKGINIRALSIADTTDFGI
                     LRLIVNKPEAASNILREDGFMVSETEVIAVDVPDRPGGLAEVLDTMGQAGINIEYLYA
                     FVGQASSEALVIFRVEDCAAALQALRAKNIRVLTGEEVYAL"
     gene            complement(24250..24753)
                     /locus_tag="VFC74_02800"
     CDS             complement(24250..24753)
                     /locus_tag="VFC74_02800"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK24298.1"
                     /translation="MRLPHSGDKKSTAAAYNRQGALMMQRGRFREASFHLLSAVVCDP
                     DCWPAFFNLGNCWVKISEYEAAIWAYEQAVRNCTEYGPLFLNLGVAHCQYGQVEQALP
                     YLEQAWRLEPERAACSAALGYVCYKLGELGLSWHWYDKALRLQPEKNEYKESIQYIGD
                     LISVAAL"
     gene            complement(24920..25681)
                     /locus_tag="VFC74_02805"
     CDS             complement(24920..25681)
                     /locus_tag="VFC74_02805"
                     /inference="COORDINATES: protein motif:HMM:NF018288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase family protein"
                     /protein_id="HZK24299.1"
                     /translation="MIDLQKTSGLPLTADKAGKLTFGPDLAAVTPAVRRKKEMLEVLL
                     DPQAEAPEELYYMYRDLCRQQDRELLAKHGLRYDVTVIRPGQIGREYIKTAGHYHPLK
                     PGTEATYPEVYEVLAGQAHYLLQTEPDEDGVDSLLVEASAGDKVLIPPGYGHITINPG
                     SAFLIMSNWVAADFSSVYQPIKDLAGGAFFEVTAEGEDQQFVANTKYQPTPRFSTRPV
                     VDQPEFGLVRGLPMYLEFLKAPAKFAFLTEPEKYF"
     gene            complement(25702..27123)
                     /locus_tag="VFC74_02810"
     CDS             complement(25702..27123)
                     /locus_tag="VFC74_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HZK24300.1"
                     /translation="MAEITFGTDGWRAIIAEDFTFTNVSYVAQAIAQHLQKEGLAAHG
                     VVVGYDARFLAAEFAQVVTEVMTGNGIHTYLCIKHTPTPAVAHAVLTHQAGGAVMLTA
                     SHNPAEYLGIKFIPDYAGPALPEEIKPIVANLQTILSSGRVQRLASEAASAQGLVTEI
                     EPLPVYTEHLESLLDFAAIKKAGLKTVVDPMYGAGMGYVESILAKHGCAVEAIHNWRD
                     PLFGGSLPDPAAEHLQELAARVQESGADLGVALDGDADRLGVVDPEGRFYSPNEILTL
                     FMEYLVKARGFRGAVARTVATTHMLDRVAASYGCPVIETPVGFKYIGQALREKNAFLG
                     GEESGGVSINKHLPEKDGIFAALLFLEMLAKTGKTAAQLLTDLERRFGPLYTARLDVR
                     TTVTAKAAIIDKMAAWQPQTIAGIKVALLNRLDGVKVVLVNGSWCLVRPSGTEPVFRI
                     YTEAATAAEKEAIQAEVQATLLG"
     gene            complement(27264..28271)
                     /gene="gap"
                     /locus_tag="VFC74_02815"
     CDS             complement(27264..28271)
                     /gene="gap"
                     /locus_tag="VFC74_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HZK24301.1"
                     /translation="MSVKIGINGFGRIGRNTFRALQGNQTVQIAAVNDLTDNATLAHL
                     LKYDSMYGKFPYEVALEGDNLRVEGREVKILAEALPEKLPWRELGVEVVLEATGKFRT
                     HAEASRHLQGGAKKVIVTTPVNDADIIMVLGLNQQQYDPDKHQIISNASCTTNALAPV
                     VKVLQENFGIRKGLMNTTHSYTNDQRLLDLPHPDLRRARAAQLSMIPTTTGAAKIIGK
                     IMPELAGKIDGFAVRVPTPTVSIVDFVAELERSATPAQINQAFKAAAAGELQGILAYS
                     EEPLVSIDYKGSPYSSTVDSLLTMVIGENLIRVVSWYDNEWGYSHRVADLAVLVAQQE
                     L"
     gene            28480..29280
                     /gene="dapB"
                     /locus_tag="VFC74_02820"
     CDS             28480..29280
                     /gene="dapB"
                     /locus_tag="VFC74_02820"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HZK24302.1"
                     /translation="MTIKVAVNGALGNMGKTVVRAVHADPALQLVAAIDVNGTDNDIG
                     PIAGIGEVNIPVSTALAATLQQTKPDVVVDFTSPFTVMQNIATALSLQVRPVVGTTGI
                     TEADLEKIANLSLQYQTSAVVAPNFALGAVLMMQFAAQAAKFFPEAEIIELHHERKID
                     APSGTALKTAEMMLKARGQAVPRQVEELEKLAGARGGNLQGIRLHSVRLPGYVAHQEV
                     IFGGLGQTLTIRHDSIDRTSFMPGVLLSIKEVMQREGVIYGLENILNF"
     gene            29351..29953
                     /locus_tag="VFC74_02825"
     CDS             29351..29953
                     /locus_tag="VFC74_02825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24303.1"
                     /translation="MLLEGKQIGFALPEGHFSMPAILDEVRKLVAAGANIYPLFLATS
                     DHPELEPHYHQTKQMLEQITGHEMMPTTLAEDVGELFSEQLAFDILVVAPCPGNFLAK
                     LVNSRTTSISLAKSVKHLQVGKPVVLALITNGDSENLLQNVQQILSLKSFFLVPFGPT
                     DEDGKQIYLSRLDLLYETVLQALQQKQLTPVYIEPCWLPS"
     gene            complement(30021..30602)
                     /locus_tag="VFC74_02830"
     CDS             complement(30021..30602)
                     /locus_tag="VFC74_02830"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK24304.1"
                     /translation="MQYLKPEIRRKILSSALAEFAEHGFARSQMRRIAQGADISTSNI
                     YRYFDGKEEIFAEIVRTVHTQVSALILSVRNESEKNGSNVKSTAQQIAEGIMKIYLAY
                     GRELLVISDKSEGSKYEHFKESLLEMVCERIKSKIFRQPSEADETVIYVLVNGFIEGI
                     FIIMRQQQDAQKMAAMINRLVSFYFDDLESRLL"
     gene            complement(30745..31080)
                     /locus_tag="VFC74_02835"
     CDS             complement(30745..31080)
                     /locus_tag="VFC74_02835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24305.1"
                     /translation="MRQRLGLCLLVFVLAALVLGFIFRQQQVAYTPIKQHYRDRWTGI
                     EWVKTYTWGGLIRVPVGIERAAQLTAAEKLTKVARLRRQDSVLTVSWYVAVIVVSALI
                     INNLTKNRK"
     gene            31791..32402
                     /locus_tag="VFC74_02840"
     CDS             31791..32402
                     /locus_tag="VFC74_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HZK24306.1"
                     /translation="MNKNPYNMAVTLELAQESFAQRNPQTMAKFSDCLYDETTHCFTV
                     PYLGQDYTVSYPAGEVTDAAHKEVDLITAILLVHYLTGASGVALTNNWISFKELQGGS
                     IYIDPFHKRAIQPMLKGFGTQPAAFTRTAALLGGQPASFGDLSYRFPVLPRIPIILIL
                     WQGDDEFPPNGNILFDQHANTYLHTEDYAHLASQTVYALLKKI"
     gene            32593..32769
                     /locus_tag="VFC74_02845"
     CDS             32593..32769
                     /locus_tag="VFC74_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24307.1"
                     /translation="MFKWSTSTADQEPLRLNNALLWTLMLIVVSALGLGLFPWLTFLA
                     TLHQYQQSLMLWGL"
     gene            32771..33280
                     /locus_tag="VFC74_02850"
     CDS             32771..33280
                     /locus_tag="VFC74_02850"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK24308.1"
                     /translation="MKHLTLTQRKTLLLVLLALWLMMLVYGIIAISSLWSRYQLFNVA
                     SGSMEPALSTSTIIAVAKNNGTLYAPGDIITYKQDGIYITHRIIELGYNKGFYYRTQG
                     DANQDPDPGLIKHQEIFGQVVFTVPAFFTPLRRILNVRSLLLFPVLLFAALYLKKNRK
                     VAARRPLHN"
     gene            complement(33377..34390)
                     /locus_tag="VFC74_02855"
     CDS             complement(33377..34390)
                     /locus_tag="VFC74_02855"
                     /inference="COORDINATES: protein motif:HMM:NF014398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0042279 - nitrite reductase (cytochrome,
                     ammonia-forming) activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase
                     subunit c552"
                     /protein_id="HZK24309.1"
                     /translation="MKAKKWLTLGLLILLSMLIAAGCGGDTKEQDSTSQDGSKKQAGD
                     NVDIKALTAQWVESKHSNILLSPAQRDECVVCHDGGAFAENITEVAAIERDFFISVDC
                     RACHTGQGADLMEEGKVTIPTAEKVQGGSGAVCMFCHNSRTAPNIEDENRSAPHYSSQ
                     ADLATGTGGMRLEGFDYGNTSAHVNIDDSCVGCHLLETEGGYASHSFKVDKVEAACGQ
                     CHQDINDLNLTAAGDYDGDGQEEGFQTEVEGLLALLQAAVGEELAGGSFESGHGAVVF
                     KDAEGAALDEVPKEMYQAGYNYLLISSDGSLGIHNPTLTVQLLQQSYKGLTGEDVPKA
                     KLK"
     gene            complement(34513..36045)
                     /gene="murJ"
                     /locus_tag="VFC74_02860"
     CDS             complement(34513..36045)
                     /gene="murJ"
                     /locus_tag="VFC74_02860"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HZK24310.1"
                     /translation="MSKQSSLIKSAGMVTVLLVISRLLGFLRESAIAYRFGTTAQADA
                     YLMASTLPLILFLAFNDAVKTAFIPVYGEYHRTEEGNALAQTVFVLLGAILVVVSMLL
                     VAAAPLVVKLLAPGFEGAQKDLTIVLARIMLPGLLFMGLTGLASGILHIKKNFFIPAI
                     TAYPSNLLIILTAIIFGARYGIVGLAWGTMAGFASQFLVQVPAVASHGVFQRANFQWH
                     HPGIKKILILLPPVIIGSAATELKSVIDRIFASLLSTGSIAALNYANRIYLLPNGIII
                     LALLTVLYPALVELFAEDKIDDFKYTLHHGIGLIITLIFPIMVGMIILRVPIVRLLFE
                     RGVFDAAATQNTAFALAFYSLGLLPLGVMLLMSRAFYATKDTLTPMLFTFVTMIVNTV
                     LNWLLMKPLAHGGLALGTATSIYVGAVGMTYLMWRKIGAFGGRKLYSTFWKTGLASAL
                     MGLLVWYGTRFLSGVSFMRQALEVGILIALGAGVYFVLIYLLKVEELQAALGLIRRRR
                     KQ"
     gene            complement(36144..36593)
                     /locus_tag="VFC74_02865"
     CDS             complement(36144..36593)
                     /locus_tag="VFC74_02865"
                     /inference="COORDINATES: protein motif:HMM:NF018410.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small multi-drug export protein"
                     /protein_id="HZK24311.1"
                     /translation="MTYLKVLVTSALPVVELRGGIPLGLYLGLTLWQAFFASLAGNML
                     IVYPWLWVLERLEEFLAKNTVTAPVYRMLINKVSKKKDMFRKYGKFALFLFVAIPLPT
                     TGAWTACVAARFFRVPKREAFWVIALGVVAAGFVILFNSYLVIDVFN"
     gene            complement(36805..37725)
                     /locus_tag="VFC74_02870"
     CDS             complement(36805..37725)
                     /locus_tag="VFC74_02870"
                     /inference="COORDINATES: protein motif:HMM:NF022702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HZK24312.1"
                     /translation="MKSSLRFFLLVLILALVVTGCGKESPDPVEQEPVAPPDDGSVTV
                     ELVDPVCVVINNHKAARPQSGMQQAHLVYEFLVEGGITRFIAVFDQPLTENYTIGPVR
                     SLRPYFAETAMEHGAAVAFSGTSGRTDEMIAHLDLQKITNGKYFWRDDSRKAPHNLYT
                     NLEKVYAARGASMAKSVKVMPQELPAGEEALEFEVRYNRNNAASYKYDSVKKQYMRFE
                     DGAAHVDRETGLQYGATRVIVRTNVHVNVAGTSLVDIDVSGSGPAVLYEGGQKYDLTW
                     EKSGQQTVYKLADGTEVDLTLGNTWIQVVR"
     gene            38152..39696
                     /locus_tag="VFC74_02875"
     CDS             38152..39696
                     /locus_tag="VFC74_02875"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK24313.1"
                     /translation="MANLFTGLSRQIVKKPVLTLFMAILIIALLLVGALKISMATGTE
                     TFIDTESQTYQDNAYLEETFGGENIVVLLTAENLDTLLTAENLAKLAAVEQTFTHSDS
                     VYTVISPATMVKEMTTKQAATMLAGVSEMKDGLTTMSSRLLDVAASIRKMKAEMPNVD
                     FSKTAASFQELTSLLDNLITGQKQLPTGIAELGSGYNQFGTLAAKTGTQIKLFSGQFA
                     QMLETLPLEDGKKNELRQQTAGLAQAGEMLTEAGSKMTQISWQAGSMAEAPLQTAQGL
                     EKMKNGLRTQVKSLQQLQQQMPDLSQLDTLAAAMDTFSQNLLTIAQGLETMLAQSNIM
                     YPGLPRSQQTLELLLYEESGELRPLFNQTIVDQQHALMMIKLQGQLSDAEKEEVVEQL
                     NNALTRNKLTSVKTTVTGKPVLDMVLRSEMRQSMQKMVVLAVLLMVAIITLIFNVRWR
                     LFPLVVNFIAVLATMGLLGHLQLPMTMVSMAAFPILIGLGIDYSIQFHSRYEEEYWQE
                     ASSNEL"
     gene            39686..41050
                     /locus_tag="VFC74_02880"
     CDS             39686..41050
                     /locus_tag="VFC74_02880"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HZK24314.1"
                     /translation="MNFKRVITKTYQSMGPAVLIAMIATILGFVALLISPVPMIADFG
                     KMLALGLVLSFLAALIILTVSLYIKDKFFSPAAGQKNYQAAPVDRLEQPLAAVTRQVL
                     RYKFVIILLALLITLGGLYGNSKVGVQTDIEQFMPQDTPELQEIRELREVIGSTEQVT
                     IMLQSEQLLTTKNIEWIDTITQELPTAFPEAIVDVRSITSVYRELNTDEFAAQQVAGF
                     IDQLPAAQRKMLIDESERASAITVNLARLETSKQESFITQLAEYLSVDNPAEIDVTIT
                     GQAVIDVQMMTALTSGRTEMTLLGMGLVFLGLLVIYRSFLKALIPILPISLIVGWSGG
                     AMYLLGFNYTPLTATMGALIIGIGTEFTIILMQRYFEEKNKGLSKDEAMIKTVGKIGK
                     PILASALTTIAGFSALIFSDFLILREFGIVTLLNIIFCLLSSLVVLPPLIVLLDRKPV
                     AQKA"
     gene            complement(41134..43710)
                     /locus_tag="VFC74_02885"
     CDS             complement(41134..43710)
                     /locus_tag="VFC74_02885"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZK24315.1"
                     /translation="MGILKNLTLRSLKLHRKRTIVTMIGIILSGAMIMGVASIAASFQ
                     DLFIQSAIQSSGNYHASFYDVDLDKVDIIAKDHSTAAVMLSRSKGYAIFAQSTDEYKP
                     YLLLKQYDRAAFAHFPTTLVAGRLPATSHELLIPEEVQKQGSYQLGETITLAFGQRLL
                     DGEQLGSDTNYQAEETFQPESEQSFTIAGVAADPLFDFSPGYTVFAHLDAAALQELDT
                     VNVSMLSNKLKQIFQEVPVMAQNAGAQDFTYNNELLRWSGVSGNRNTMNFLSSLTLII
                     IALIVVGSVAVIYNAFAISVSERKKQFGMLASVGATAKQIQSTVFYEALLLGLIGIPL
                     GIIAGLGGIGVTLKVVNQLMMGSMFSEELALRLVISPNTILITIAFEALTILLSAWLP
                     AKRAARISPIEAIRLTDDLKLKGKEVKTNKLASKLLGVEGELALKNMKRNRKQYRATV
                     FSLVISIVLFVTFSSFMEYGFISSGLYYGDIAYNVVVSSDYGEDSEGQNQFYQQVLDL
                     PEVERAAVARTLYSQTKLAAEQLGDYLQKNFLQAEYFPQNEQGQYQLNVELVAIGEAE
                     FTAYAAELGLKPEDYQDPQRLKGILLNKNVLDGKVSYQPLKVKAGTELSFVDRQYEAD
                     SLPAEFDLTIGALSEELPFGVNFTEMGLKIVVSDEVFTAIAAQLPAESQLQAQQAALY
                     LSSSDSVALVEKIKVLEEQVEADFYMQDISQMQAEMKRTTTVMAIFLYGFVTLITLIG
                     VTNIFNTISTNVALRRREFAMLQSVGLTPQGLQKIIRFESLFYGFKALLYGLPLSILI
                     SFWMFNAFSNLFTFTFIIPWQEIIICILGVFLVTFLTMAQASAKFKQQNIITALGSEL
                     VF"
     gene            complement(43698..44390)
                     /locus_tag="VFC74_02890"
     CDS             complement(43698..44390)
                     /locus_tag="VFC74_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011987812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24316.1"
                     /translation="MEILRVENLTKVYGSGTTQVKALGGVSFSVKQGEFVAIVGASGS
                     GKSTLLHSLGGVDRPTSGKVMIKDTDIYQLNETKLAVFRRRHLGLIYQFYNLIPILNV
                     EENITLPLLLDKQEVQQKYLQELLQTLGLTDRLHHLPNELSGGQQQRVSIGRALINHP
                     ALLLADEPTGNLDSKNSAEIIALLKLSNKLYQQTLIMITHDQQIAQQANRIISLEDGQ
                     IVSDEVIRPWVS"
     gene            complement(44557..45069)
                     /locus_tag="VFC74_02895"
     CDS             complement(44557..45069)
                     /locus_tag="VFC74_02895"
                     /inference="COORDINATES: protein motif:HMM:NF021363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2062 domain-containing protein"
                     /protein_id="HZK24317.1"
                     /translation="MTEETNLSSWEKIKKRFEETIEKQIKKDEPRKVALGCVLGLGIN
                     FFPTLGLGFAFAFLLAMLFRANRASATVTSLVTGPFIPLMYALNLLIGGIVLTPVAGH
                     ENLREFIIHQYAIILKRGDLQEKIFGFLEFFGSTFMVGAAINATVFGVACYFFVRHML
                     IKKAKRTAGK"
     gene            45756..47108
                     /locus_tag="VFC74_02900"
     CDS             45756..47108
                     /locus_tag="VFC74_02900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZK24318.1"
                     /translation="MANAQRNDVGKTEIWSYGFGALGYNLGAAGLMTFLTFFYTDYML
                     IPAASVASILFFSRLIDGGTDLLLGVLIDRTKSKYGKARPWLLWMAIPAAISVSLLYY
                     VPNFSTSGRVIYAFITYNAVAFFYLTALALPFQALVSLITPNFQTRISLSQMGAIFNT
                     LAAVFVNLFARKIMAALGGEAVGLWRFFTLMGVIGMGLMVLCFYMTKERVVRKENENN
                     IPLGTGLKALAANKYWWNITLLQLVTSLVPAMWGATVYYAQYILNAPGMVGPIMSLMW
                     GGITIGIFLFMPITKKYGKRNAAQMGMILQVLGSVVLWFNPTSSALLWISTTLRSIGP
                     AAFIAAGNAMRADTVEYGEWKTGIRNEGMIYSGASFGAKVGSGIGGAILAALLARGGY
                     VGGAAVQSAAAMEAIKTAFIIVPGIGSCLIMVMLAFFDLEKKMPQIMSDLEERKKTAA
                     "
     gene            complement(47248..48303)
                     /locus_tag="VFC74_02905"
     CDS             complement(47248..48303)
                     /locus_tag="VFC74_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK24319.1"
                     /translation="MKNTKQHILATAKHLFYEQGYEATTIKDIAEHVGIANSVIFHYF
                     KNKRDILIHVFKDYDKTIIKLGRSLGPHLNPIERSVLVSKMYLLHKKYDEKTQQMFLT
                     SIKENIVTELLYQNFFQTFKELMTFQGINAEEDPLLAFRYMSFIGSIEQIQLNFINGK
                     IKFTEENIDLSIMARFKFIFGVEPGVIKAALQKVKSVLNAVTFSSFDVFVTEDQLTKY
                     LYRHYETNFTEIVVASIDCLRAQEDLGAALKERYQRLIVITKNYPLGNVVDTVAIETS
                     LGDFKKKVISLYEELKAKFTEQEIFLTGVVDSKEARIKPVEDYGIEVAETLDQLKVDY
                     YLGNKTITHCALLFYTT"
     gene            complement(48455..49441)
                     /locus_tag="VFC74_02910"
     CDS             complement(48455..49441)
                     /locus_tag="VFC74_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK24320.1"
                     /translation="MLSKRQNMLETIKGGKPDRFVNQYEYLGIVMGDPITARNPRASL
                     GGENVDFWGVTHRWPAHVPGSFPVHDQEHKLIKDVTKWQDVLKAPSLESSPEDWKGIQ
                     AQVAKIDRNEQFVTIFGAPGIFEQTHYFMGMDDTLINFYEEPEAMHELIDFITEWKLG
                     YAKEIVKYVKPECLFHHDDWGSQINSFLAPDMFAEFFVPAYKKLYSFYKENGVELIVH
                     HNDSFSANLVPHMIEMGIDVWQGCMTTNNTPELVKKYGGQISFMGDLDNGLLDREDWS
                     RELIAKEVRRACETNGKHYFIPCLVRGLSGSTFPGVYEAVSEEIDKMSKEMF"
     gene            complement(49650..50354)
                     /locus_tag="VFC74_02915"
     CDS             complement(49650..50354)
                     /locus_tag="VFC74_02915"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018590139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZK24321.1"
                     /translation="MRRIEHLYEDNHLLVVEKPVNLLTQGDNTGDDDLLSLLKQSLKE
                     KYQKPGKVYLGMVQRLDRPTGGVIAFAKTSKAAGRLAAQLRDRTFTRAYLAIVHGKLP
                     LGQASLQHYLRKNRQNNTVTVVDKNSSGAKEAKLTYQVLDNSKNFSLVEVQLQTGRAH
                     QIRVQLAAAGHPLFGDQRYGQSVNRPGQQLALWSYCLTFTHPTLQTRLTFTSYPAAKH
                     PWTLFAYESLVGQATS"
     gene            50648..51328
                     /locus_tag="VFC74_02920"
     CDS             50648..51328
                     /locus_tag="VFC74_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HZK24322.1"
                     /translation="MKCNCHQHGIANCIELVPIFSNLSTEEKKEVAAITRDKTLVKGE
                     MVYLAGDKGEKLYVIHEGFVKISRLSPTGKEQVIRVLGPGEFMGELTLFSSLPMTDNA
                     EAIADTIMCMIEGPQLKKLMPKYPAIAFKIMEELSYRLEKAENLIEDISLHSVEQRLA
                     QSLLSLSQAQPKITLKMTKSDFASQIGMSQETLSRKLASLQEQGLIKLVGQRQIIILD
                     RQGLEALV"
     gene            complement(51393..51641)
                     /locus_tag="VFC74_02925"
     CDS             complement(51393..51641)
                     /locus_tag="VFC74_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HZK24323.1"
                     /translation="MQDKDMVSDVLSQVSASLTNYATAIAESENSQLRQALIQIRNAD
                     EQFQFQLSQKAMEKGYYQPAKQADPNAIQQYKSQLSSS"
     gene            complement(51657..51839)
                     /locus_tag="VFC74_02930"
     CDS             complement(51657..51839)
                     /locus_tag="VFC74_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24324.1"
                     /translation="MTINQQELENLRHLIGGHATGANKLQAYAQQCSDPEIKQMFQDS
                     SSRAQQTKQKLMQFLN"
     gene            52033..53304
                     /locus_tag="VFC74_02935"
     CDS             52033..53304
                     /locus_tag="VFC74_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009324521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24325.1"
                     /translation="MFNPFDEKPMLLGEAIMDWKTIYPQAYDKNLVDPYSKVRIILMN
                     GIEVEAALFSHQFHRNCNDNELRRDLAFSRRIEQQQQKRINWLSPIGETPLETTIGYE
                     HVAVDLTSWLAQNEPDPYVKDTLDFALLEDFDHLYRYANLLELDKNISAQKLVKKYVE
                     ITPGRPTIAEHRHPSDTVRGYINFKSADIRTKLNIMIITAAEQQTMNFYMNVGPTYYN
                     DLGRQLYQEIALIEEEHVSQYGALMDPNCTWLENLLMHEYMECYLYYSFYQDERDPHL
                     KNIWEMHLQQEIAQLHRAKDLLAKHEHKDWQQVIPGGVFPKLLQFHDTKDYVRKILGQ
                     QIELTADQEVLKNVNDLPDNHRFFWYQQTVNHDVKAVPSHAVIAQHQQQKGQDYRYED
                     QPNAVEALRDRRQDNFKIARTKQKQRQLVGV"
BASE COUNT        14487 a        13397 c        11042 g        14471 t
ORIGIN      
        1 gttcatttta gcttacgttt tttatctggc aatatgttaa acaatcgctg tctcctattt
       61 ttttataaca tttgtcgcct ctgagccgcg ggtacattag gctaaggcct caacatagct
      121 aaatgatcat attttgctgc taaaatcaac gacagcagcc ctctggctaa gatctcatga
      181 atgagcaatc acggcgagat agacttcctt taccccggct tggtgcagcg cctcagccgc
      241 aaaatgcgct gtggcaccgg tcgttagtaa atcatccacc aacagcacac gccgcggcac
      301 acttggttgt tgatgaaaga caaaaacatc ggctacgttt tgccagcgct gtgctgcact
      361 taatgtagtt tgactgacag tagcttgccg acgcgttaaa attctttgca gtggcaggtc
      421 taattcttta cccaatactt gcgctaagag cagggcctga tcataaccgc gttctcgtaa
      481 ctttagcgga tgtaaaggaa tgggcacgac actatctaaa gctggccact ggcatttccg
      541 cacttgccta gccatcagct gaccaaaaag gaaagccagt tctctcttcc cgccatattt
      601 aaaacgtaag attgcttttc ttaactcacc ttgaaaaaga ctaagcgcac aggaggcata
      661 aaaggcatag ctgtgctcgc tgcacagcgg acaaaagccg tcttctgagg ccgccaacgg
      721 atacccgcag taactgcagc agctctgtgg gttgagaatt gtctgttcac agctgcggca
      781 cagtaacaca cctggctgcg cctcgttaag cagcgtacga caaaaaccgc agcgtcgtgg
      841 gaacaaaaga tctaaaaggg gtgttagtag gtccacctgc atcctcctgc ttagcgataa
      901 tttacaagtc ttgtcgctaa ccctgaatag cgggtcgcta ctttgttgtt acgcaccgct
      961 atcgctagcg cttttttcgt gccaattagc accactaact cttttgcccg ggtgatagcg
     1021 gtatacaaaa gattcttttg caagagaatg tagtgctgtg taaccacagg cagtactacg
     1081 atggggtact cactaccttg gctcttatgc acagatgtag cataagacag tactaactcc
     1141 tccagctcag cctgatcata caaaacatcc cgtgccccgc ccacatcagg atacagcacc
     1201 attacttcac catcatcgct gttgagatag gtgatgcggc ccacgtcccc attaaacact
     1261 tctttctgat aattattgcg tatttgcatt actttatcac ctaagcgaaa agtggtgcgg
     1321 ccaaaagtca gctctggttt atggggagca ggcggattca atctttcctg cagctgtaca
     1381 tttaactgat ccacacctaa acggttacga cgcatgggac ttaaaacttg aatatcagca
     1441 atgggatcac gatttttata ctgtggtaaa cgagaactgc ataagtccaa aacctttttt
     1501 aaaataattt ccgggtcaga ctcttgaata aagaaaaagt cttcgctgcc gcttaaaagc
     1561 ggcagctgtc catcattaac ttgatgggca ttgctgacaa tcatactatt ttctgattga
     1621 cgaaaaatct gcgtcagccg taccactggt acaacttcag aagtaatcaa atcacgcagc
     1681 acatttcctg gcccgacaga aggcagctga tccatgtcac cgacaaggat tagtttacag
     1741 ccctccggaa ctgctttaag cagataataa aagagcggta aatctaccat cgacacttca
     1801 tcaataatta agacatgagc ttgtaagggc cggtcttcat cacgctgaaa agcaaaacct
     1861 acgccgctgc tttctttttg tgcgtattcc aataagcgat gaatagtttt tgcttccaaa
     1921 cctgtcgctt ctgtcatgcg tttggcagct cgccccgtcg gagccgccag caatacctct
     1981 agtccgttgc ggtgaaagag cttgattaag gcgcgaatag tagtggtctt accagtaccc
     2041 gggccgcctg tcaccaccag cagccctgca tccattgctt gctgtacagc cagctgctgg
     2101 gcggcagcca aagtcaagtc atcctcctgc caagctgcct tttctacaga cgccaagctg
     2161 gactgcgagt tagcaagctc taaaagccga gccgccacat aacattcagc ttggtaaagg
     2221 ggggtcaggt aaattacagt tacgccctct agttcagtaa ggacaatttg cttactttca
     2281 gccaacccct cgctttgctc acttaccatc tccactgtta ccggttccgt attagtcgac
     2341 aatgcgctga ccacagtatc cgataattct gtctgaggta aatagacatg accttgctct
     2401 tggccttggg taagctgata caagatggca gctctgatgc gatggggaga attaggtgct
     2461 aaccccattt ccaaagcaat cttatcagcg gttttaaagc caatgccaaa aacatcactg
     2521 gctaggcgat agggattttc actgactcga gcgactgtat catcaccgta tctccgatag
     2581 atacgcgctg cataaccgat gctgacgcca taactttgca agaaagtcat tactcggata
     2641 atctcctgac gttcctgcag actgccggca atgagagcag attttttcgt gcctatcccc
     2701 tcaatttcgc atagcctttc cggttcttct gcaataattg tcagcgcttc taaaccgaaa
     2761 tgctccacaa ttttttccgc ggtagaaggt cctactcctt taatcaaacc agaagctaaa
     2821 aaattgcgaa taccttttat attatggggg gcttccgcct catagcgagc tactttaaac
     2881 tgacggccaa agtctcgatg ttgagtccac tccccctgta ccaccagtct ttcccccaca
     2941 cttaagcgcg gcatattacc aatgatggcg tgcagctgct catcatcact gcgcagccta
     3001 gccaccataa ataaagtatc ttcattatag tatgtaattc tttctagtgt acctgctaaa
     3061 ctctccataa ctttccctcc gcctgtctgt attccacatt acatgcttgt ttcctgcttt
     3121 acagctgcct gcaccaggta aggcgcgcct ccatctccag gcggtagtta tgtacgggag
     3181 gatgacctcc cgttatttgc gcgtggctga attctatctc ccttgcctct ggccagagca
     3241 gccagataaa attgcagcct gaaagtgcag cctttattgt tcataagacg ccaggtcttc
     3301 actatcacgt gacaccaaac gcgcccgtac tttctgcact aaagcgcccc ccgtactttg
     3361 gaagagattc ttgcttagca ctagatccat gaccgtagta atttcggctt ctgttaaatc
     3421 tgcacgaggg ttaggaatac tgatggtaaa taagcggcca tcttcatttc tgaagattag
     3481 ctgcagcgtt gtatccatca tctcacctcc ttaccccggt tctttttgct tattctgcaa
     3541 tcaattcacc atcctccaaa cggcgaagag catagatact gaagccttgc agtgacatta
     3601 atgcagtaaa gacctcatgc aaatcatcgt tggttactgc aggcagaaca cgaccatagg
     3661 tgcggctcac caccacagga ttgccctcat catcgacacc gctttgcagt ctaacttgac
     3721 aacggctata actaggttct aaaacgacag ccatcacgat cacctccttg cctcaaactg
     3781 ctttgattat aactgctgag agcaaggcgc ggcaaatgtc aactttgggg gcaaaaccac
     3841 cctagattga gcagcagctg cgcaaagttg actcgccaaa ggccactttt gctcaaactc
     3901 atcaaattta taaaaagtgc gcccattcat cttccttagc gcgcactttt tatactggct
     3961 tcttaaaggc tttgctgact gcaaagtgat aatttatgcc agtaaaaaaa agcttgcact
     4021 ttcaatttcc cttagcaagc tttttatact ggccacttaa aggctttgct gactgcaaag
     4081 tgataaattt atgccagtaa aaaaagagca atgcttgtcc acattgctct tctctgcttg
     4141 cttatttaca ggccagcttc atcttttagt aaagcggctt tatcggtttg ctcccaaggc
     4201 aggtctaaat caagacggcc aaaatgacca taggctgcaa cctgcttata gataggacga
     4261 cgcagctgca gtttctgaat gatcgccgct ggacgtaagt caaaatgctg gttgactaaa
     4321 gcttcaatct tttcctcggc tactttagcc gtgccaaagg tttccactaa aagcgaaatt
     4381 ggatgtgcca caccaatggc ataagccaac tgtacctcac acttgtcagc taaaccggcg
     4441 gccaccacat tttttgccac ataacgtgca gcataagcgc ctgagcggtc aactttcgtc
     4501 ggatctttac ccgaaaaagc accaccacca tgacgagcat aaccgccata cgtatcgaca
     4561 ataatcttac ggccagttaa accggcatcc ccttggggac cgcccacgac aaaacgacca
     4621 gtggggttca cataaaagct tgtctgcggt gtcaggtatt gtgccggaat cacttctcta
     4681 atcacttgct caataacatc gtttctgata cgggttaagg atatgtcttc tcgatgttga
     4741 gcagctacca ccacagtttt gatgcctaca ggcttaccag caagatactg cacagtaact
     4801 tgtgttttgc cgtctgggcg caaataagga agcagttttt gcttgcgcac ttctgccaag
     4861 cgccgtgcca gcagatgagc catggcaatc ggcagcggca ttaactcagg cgtttcatta
     4921 caggcataac caaacatcat cccctggtca ccagcgccag ttatttcaaa ctcgagatcc
     4981 tgatttgtac tctctttaga ctcaaaactt tggttcacac cttgtgcaat atcgctggac
     5041 tgctcatcaa tagaagtaag cacagcgcag gtgtcaccat caaaaccata ttttgcgcgc
     5101 gtatagccaa tctcttttac tgtttcacgt actattttag aaatatcaac ataacagttt
     5161 gtggtaatct cccctgccac caacaccaaa ccagtggtaa cggatgtctc tgccgccact
     5221 cgtgccagtg gatcatgagt caaaattgaa tctaaaatag cgtcggatat ttggtcagag
     5281 actttatccg gatgtccttc cgtcacggac tcggaagtga aaagataatt cttgcccatg
     5341 tctttaatgc ctcctctgga tatgtttaca caatttgctt catcttaacc atcaagtcaa
     5401 tcctctgggc tgcaacacaa aaaaggcagc agtactggct tcattagcgc aatcagctat
     5461 tattgctcgc cggtacaaga aaaaacctct tcggatgcaa gaggtttctt ttaggagtaa
     5521 cctctcctat ctaccaggca aattgcctgc tggaattagc accgctaaca aaatgtccgg
     5581 ttgctgggtt tcatagggcc agtccctccg ccgctcttga taagagtatc tagttttttt
     5641 tctttatcgt ctgcaggacc aatgtcatga cagcttactc tgtcacaaca atcttaataa
     5701 taaccgaggg attctatctt tgtcaaacat ttttagctgt cagataatta aaaatgagtg
     5761 aggtgcaaat taagcttggg taattgcaaa cattaattcg ccgctggcca ctactttacc
     5821 gttaacagtg gcagtcccct tacctttacc gatggtaccc cgtaatttaa taacttccag
     5881 ttccagctcc agcacatcac ccggcacaac ctgctgctta aagcggagct tatcaatccc
     5941 ggcaaacagt gcaatcttgc cgcgaaattc tggcatactt agaatagcta ccgcacctac
     6001 ttgggcaatg gcttccacca ccaagacgcc aggcataaca gggtaaccgg ggaaatgacc
     6061 ctgaaaaaag ggctcattaa tcgtcacatt ttttatccct ttagcccgct cccccggtac
     6121 aacctccagc aggcggtcga ccaataaaaa tgggtaacgg tgaggtaaaa tgctttgcac
     6181 atcccatgct tgattcatgc tgttggcctc cttactcatt ttccagcaaa gctctcatct
     6241 ctgccaattc tgttgctaat tgctgcagct ccagttcagc atctccttct agtatagcca
     6301 attctaaacg tttactgtaa tcacgctgaa aatcttgcca caactgctgt aaagttttag
     6361 cgctcgtctg tcttaaagat agctctggac gtttatcggc aaccgcctcc gtagtcagtt
     6421 ccacagtctc taaatactct ggaatataag tgtttaagtt cagttcccgc tcaatcaggg
     6481 cagcatactc tggcagcaca ttctcttctc cgtgtactaa aaacacctgt tggggtttct
     6541 tggggaatct tcctaaccac cgcaatagct ctgaacggtc agcatgggcc gaaaaaccgt
     6601 caatactgac aatgcgcgca ttcactttaa tgtcttcacc aaagattttt acgattctgg
     6661 caccatcacg gatacgccgc cctaaagttc cctctgcttg gtaacccaca aaaagcaccg
     6721 tagattcagg acgccataaa ttatgtttaa gatgatgctt aatgcggccg gcatccgcca
     6781 tgccgctggc agagataatg atcgctcccc caggactttt gttaattagc ttggactcct
     6841 cagctgtttt agtaaagata acttcaggga aattaaaggg atcttgaccg ctcttgagca
     6901 attcacgcat ctgcagatca taacattccg gatggcgggt gaaaatttcc gtagctgaaa
     6961 tcgctaaagg actatcaatg taaatgggca aattggggat ctggtgatca cgcatcaggc
     7021 ggcttaaaaa atatagtaat tcttgcgtac gccccaccgc gaaggagggt ataactacat
     7081 taccttgagc aagtattgtt tgctgaataa cctgctgcaa gatctctatt ttttgatctt
     7141 ctatttcatg ttcccgtgtg ccataagtgc tttctatcac cacatagtca gcttcttcaa
     7201 ggatatcggg atctttgata atgggctgtt tggctttacc caaatcacca gtaaaaacaa
     7261 ttttctctgt gcgttcagtg ccgacgatag tcagctcagt aatcgccgaa ccaagaatat
     7321 gaccagaatt atagaaggtc aaagcaatac ggtcactcaa ccaaaacttt tggtaataat
     7381 tcacttcctt gaaacgtttc agcgctttct cggcatcttc tctcgtatac aaaggatcaa
     7441 ttaagggacg cccggcacgc aaattcttgc ggttttgcca ttcagcctcc atctcctgaa
     7501 tataggcgct atccggcagc ataatactga ccaaatcaac tgttgcggaa gtcgctagga
     7561 tgtgaccacg aaagcctcct tttaccaagc ggggaatcag gccagaatgg tcaatatgcg
     7621 catgagaaag gatcacaaaa tcaatctctg ccggattata agggaagtca ttaaaattac
     7681 gagcccgtaa atgtttattg ccctgaaaca taccacagtc cagtagtatt ttctgccccg
     7741 ccgcttccaa gagaaacgag gaaccagtga cggtttgcgc gccaccacaa aatgttagtt
     7801 tcatatccat tcctccggtt tacttgtctc tagtagtgtc tacttttatt tataattcgc
     7861 ttctctcgcc tctaatcctt tctctgacta gatcaaaact ggcagacgtt ctgccagcgt
     7921 catcccagct tggtcaaccc gacaacggta ggcataaagc tccacgccag cgctggctgc
     7981 ctgccgcaga ctctggccaa actgtaaatc agtacgccga ttaggcgcaa aataatctgc
     8041 atctcctcgc tggataataa agattaccgc acagcggcaa ccctcccgct gtaagtcagc
     8101 caaatgggct aaatggcggc gtcctcgggc cgtaggcgca tcaggaaaca aagccacttt
     8161 atctatcacc aaagttactg actttacttc cacataaaca gcctgcccgg cctctgccag
     8221 gaggaagtca aaacgtccgc tgctgtaaac aggctcacta cgcacaaatt cacactgctc
     8281 aaaagggggc agagccttgg ccaaaagcac ttgtttgact aacttattcg gcagttgggc
     8341 atcaatagaa acccaggtct gctggtacca cactgctcgc acactaaaag ctgtgcggcg
     8401 tgtggctgaa ttattacgct ctaataacac actggctcct ggcaccaata attctgtcag
     8461 acgtccggat gtagcaatgt gtgctgccac cgtttcgccg ttcaactcaa ctaacgctac
     8521 aaaacggttt aagcgttcca caaaaaaacc agcttctgta ggtttaaaag gaagatgtaa
     8581 tgtcacgtta ttacctgcac tttcatgata gccttaatct agggaacctt aaccatggta
     8641 actggagact cgtaccctgc taacaaaaaa cgatatgaaa aacccctgac cagcaggggt
     8701 tttaatttat aatgtcagtt tctgcaggat ggcgtttaca gcctgtactg cggcggagtc
     8761 atcgggcagc tccattaacg cctgcccctt gacgtcatat tccgcaactt tcggatctag
     8821 gggaatagta ccaagaaact gtaagcctgt tttatcaatc tccggctgca gtacttctat
     8881 ctcattgccc gtgacttttg tcacgatcaa gtacatttct ttgatatcta aattaacaga
     8941 ctcggcgatc tcacgcaccc gtgcggcaga gcgaatactc cgggccgcag catcgctaac
     9001 cacaattagc atatccacag cggaaatgat gcgtcggctt aaatgctcta aacctgcttc
     9061 attatcaacc aaaacataat cgtagttact gcgtaatttc tccaaatgct tgcgcagtaa
     9121 atcattcggg taacaatagc agcctgaacg ttgggggttg cccatcacca gcaggtcgta
     9181 aaaatttgtt tccgccaggg catcctgtaa tttaaactcc atatacattt cttttgacat
     9241 cccggtaggc acagcattag gtgtttttgt atcttcaata atttgcgaaa tagtgttttc
     9301 cacttctaac cccagtgctt catttaaatt agcattggga tccgcatcca ccgctaaaat
     9361 tggtttgcct gatttatgct ccagcaaata gcgcatcatc agcgccgtaa aagtagtttt
     9421 acccactcca cccttaccag caatggcgat ttgtttcatt gccttctcat cctttcatgt
     9481 ttaagcgata attttttgtc gcataacttg ttctaaagct aatagtgtgt tacagggata
     9541 catccgtacc acctgttgaa aagcctccac tgaactggcc ttctcccact caggggccgg
     9601 caaagtgttc agccacacta catctttaac cattctcttt atttccgcca attcctgcac
     9661 tgcctgtgtc actttcattg tttttgtgtc actgacaatt aagaggtatg tttgactgct
     9721 caagaggtgc ggatagtttg cacgcaggga ctgcagagct gtcgccagtt ccgtgccccg
     9781 cccccattct tgtgaacggt tcatcaattc taccatcgtt tgtgtaaaat cggtgctggc
     9841 ttgaaaatag tcagtaacac gctccacatc ttccgcaaaa acaaaaccct caatactgct
     9901 gaccacactg tgaatactat agataaattg cagcacgaag ctggcataac gggccatcga
     9961 accggacaca tcacacaata gtaatagttg tggtttttgc cggcgatgat tacggtaacg
    10021 taatttaaac atggtgccgc caaaacgcat attatggcga atagttttac gtacatccag
    10081 ctgctgccgc cgcttacctt ggcggaagcg tcgttcgata cgtgttacca gctgccgcgc
    10141 tagtttttgc agcaaaagct gcgcccgcgg caaatcctgt tcagcaatat tttgcatatc
    10201 ctcggcgagc agtgaatcag ctgccgcttg cggttgttcc tccatactgg ccagtaaggc
    10261 attaagttcc gggtcgtcag actgcggcaa ggctaactga ccaagttcat tacgcagact
    10321 tttacgccaa aaagtaagcg agcctctgac catgctttcc aggactggac ggaaatctgc
    10381 ttccacctgc ttgccctctg ctgttcgctg aatataatct aacatctttt gtcgtgccgc
    10441 cggcggaatg gcagcataaa ctagtttttc ttcctcagat aaagccaggg ggacatcacg
    10501 aaactgcagg tcttcgctag cttccgctaa catctcctgt cgctgtgcca atttagcagc
    10561 gtattcctgc tccagctcgg ctcgaacttc cggtgaggca aagaaacgag caaaggcttg
    10621 tgtaaatacc gcctgctctc ccggacgctt gaccagcgtt gcctgtaggg ccaggcgcac
    10681 agcatcacgc tcggaaagtt ccacctcctg cagcgcttgc agcgcatcaa taacttcgct
    10741 gctgccaata cgcagcccca tgtggcggag cagctgtaca aagcgcacca catttacttc
    10801 caaacggttg gtcatcatta ttcttcctga cttagcgccg ccaaacggcc agcacctaaa
    10861 cgctcggtaa aggcatcctg atctgttttc ctttttagca gcagacttag cgtttgttta
    10921 actgttactt catctaaact gtctttacca agagtgacca acgcccgagc ccaatccaaa
    10981 gtttcggcaa tggatggctt cttgcgaatc tcctgctgcg cacggatttg cgccacggcc
    11041 cgtgccaact gtttcgccag cttttccttt acttccggaa gacgagcacg gataatttcc
    11101 acttcgcgct ctacagtagg ataggttaag tacaaatata ggcagcgccg ctttaagcca
    11161 tccgataatt cacgttcatt attactggtc aaaatcacca tgggaatttg ctgcgcctgc
    11221 agtgtaccaa gttcgggtac cgacacctga aagtctgaga gcacttcaaa taaaaaggct
    11281 tcgaattcct catctgtttt gtccacttca tcaataagca gcactacttt ttcctcagct
    11341 tgaatagcct ttagtaaggg gcgggctaaa agaaaatccg ctgaaaaaat gtcctcttct
    11401 agttccgtgg cgctggtgga ttctttagat agctgaatct ttaatagttg tcgctggtaa
    11461 ttccactcat acagcgcttt attctcatcc aagccctcat aacattgcag gcggattaac
    11521 tcggtctgga aaacacgcgc caatactttg gctaattctg ttttaccaac acccggttcg
    11581 ccctcaataa gcaaaggttt ttgcagtttt aaagctaaat aaacagtcag caggacatcg
    11641 tcatcggtaa tatacccatc tgtttgaaag gctttacgta aatttgctac tgtaatttcc
    11701 acggtttctc tctccctaca gtttctgtta ttattatagc gtcaccgaca ggtattaggc
    11761 atgacttata tcacactgtt aagctttatt tttgtgatca agataggcta ccgcactaag
    11821 ggcggcaatc tgcccctgtc ccacagcttt agccacctga taaggtctgc ctaccacatc
    11881 tccacccgca aaaacacccg gaatattggt ggccatctgc agatcaactt taatatgttt
    11941 atcttctaaa gccaaacctg gaatcaactg tgtcagaggg tactgctctt taagcataaa
    12001 cacgccctcg accttaattt tgcggccatc tttcagttcc atttcctcca ggctggcgga
    12061 gccagtaaac tgcctaatct tctggcctgc gagcagctca acattagccg gcagcgacat
    12121 gctcacctca tattgcggca aaaaatatac tttagcagct acttctgtta ataattttac
    12181 ttcatcctca tgctctgcac tatcactgct aacagcaata gttttgtcgc ggaaaaacat
    12241 ggcatcacat gttgcacaat acgaaatacc tctacctaaa tagttatctt ctcccggcaa
    12301 ccgtttgcct gccgagacac cggtcgtaat aaccaccgtc ttggcttcta ctacatcatc
    12361 gcgcagctgc aattgaaagc tgtctcccag cgggtaaacg gcactaatac tatgatgtga
    12421 aacttcgatt cccgctgctt tagcgtgttt taaaaatgat tgcagcattg cttctccgga
    12481 tatattatat agacccagat aattattaac ttggtgggct ttatgcagcg cttggctgca
    12541 aattcgcggc cccaccaata agagtgtacg gttacgcgcc tgtacattaa tggctgccga
    12601 aagacctgcc ggtccacaac caataatcgc cacatctact ttttccatca ctacgcctcc
    12661 tcttctacta atggtacatt taccatttac ctgctttttc ccttcatttt acgctacgat
    12721 atcactctac atttagttaa gtggccacgt aactaaaaaa acgcacagcg tcttcttatc
    12781 tcaggatgct taatataaaa ggtatctata cctatcagtc cagctttaaa aagtttggca
    12841 gatatgttac aatgtggata ataatgtaat ttaggaggct aaccgatgcc cacaattgat
    12901 tatttaggtc acgcttgttt tctggtgaca ggccaagagc acaccctgct ttttgatccc
    12961 tacctcaccg ataaccccca agcggccatc agtgctgcag aagtaaaacc ggattacatc
    13021 cttgtctctc atggtcattt tgaccacctc ggcgatgctt ttgccattgc ccggcgcacg
    13081 ggtgcaacca tcattaccac gctggattta gccaaagaat gtgaaaagca gcagctgccg
    13141 actcatgcca tgaacatagg ctgtaaacat aattttgctt tcggctatgt acgaactacg
    13201 caagccttac accatgccgg tcttgtcggt ggtgaagcct gcggttttat tgttaatctt
    13261 gacggctaca ctttatattt tacaggtgat accagtctct ttggcgattt aaaactctat
    13321 ggcatcattg agcaccctga tcttttactg ctgcccattg gcggctacta tactatggat
    13381 attgacgatg ctgtctacgc cgccaaacta gtcaacgcaa aaactattat tccctttcac
    13441 tataatacct ggcctttaat cgaggcagat cctgccgtct ttgccgcaaa ggtggcaaaa
    13501 catacttcta gtcactgcgt gatactggcc cctggtgaaa gctgtgattt atcttaatgg
    13561 aaaaaggctg acctaatctt ctcgtttagg tcagcctttt tgcgtttgta cttaattgtc
    13621 cataccttcg atttcttcgt ctaggtccat gattttagtc agaagttcaa ttctggttgc
    13681 tcctgtagct aacatgtatt cacgtaaaag accatcacga acttcgttta ggttgtaggc
    13741 atacgtagac atgtgttccc ctccttttgg ttcttcccat aattttccct attctatctt
    13801 tagttattca tcagaatatt gtactctttt tccccagcta aggtgcggcg gctaaggcag
    13861 tctctcgtga atacattttc tgatatgtgg cgcaaagggt acatgtatat acatgtacta
    13921 aaagtcttaa cagttacaat cttaacatat gtgaaattac ttgtctagcg caaattgaaa
    13981 aaaaagcaga aaatgttcac ctaattcact ttttgttaat aaatagcgtt aattaacaaa
    14041 ttaccaacgc attgcgacca gctccgacat aaaaacagct gtgataccag taaacatcag
    14101 cgcaacttac ttttcaagct acttttttct gaatattatg acttggaaga attaattaag
    14161 tattgtataa ttataagata aggaggtctc attgtaaagg gggtaaatat tacacaaata
    14221 ttaatagatg tatgtccgtc gtgtggtaat gaaaaaaaga ttcgaaggag agattatgcg
    14281 atgtctaaat tgacaccaaa agaaaactat ctgcgtttag gcaggggtga gatgccggaa
    14341 agtgttgtgc gtttcaacat gggaatgccg acaatttcag gtgaaatgac gacagcaatg
    14401 atcggtccga gcatcttaat gccgggacac ctcagtccca aaggcggcaa ggataattgg
    14461 ggcgttacat atgtaacaaa tgaagaaaca ggctttgcct ccatacctga acccaacaat
    14521 ttcatgctca aggatattac caagtggcat gaagttatca aacgaccaga gctgccagaa
    14581 agaattgact gggagtatat ggccaaaaag gattacgaaa aatccaaaat taatcgcaca
    14641 gaatcagcgg tcatggcagg ttgtggcctg atgcctttcc aacagttaat ggcctttatg
    14701 ggctttactg agggattatg tgccatgttt gaggagccgg aaacagttaa agagctgctg
    14761 cattttatgg ctgatttcta cgtaccaatt atcgataaaa ctctcgacta ttatcagcct
    14821 gatatttttt atcttttgga tgattctgcc gccaagctca atccctttat ctcggtggaa
    14881 atgtatcgtg atatcttaaa acctatctac gtaaaactga ccaatgctgc cagagagcgc
    14941 ggcattccag ttcagtttca caattgtggg cgctgcgaag atttcatcga ggatatgatt
    15001 gactttggtg tagtttacac cgaccctgct caaaccacca atgatctgct ggcggtgaag
    15061 gagaaattcg ccggtaaaat cgtgctttgc ggcggctggg actggaaaat gccagataca
    15121 tggcccatcg ttgacgaaga ggctgtgcgg cagtccgtga gagatactat tgataaatac
    15181 gccccgggcg gaggctttgc tatcggcggc ggcgttttag gcaaatataa cgaccccgtg
    15241 acgaaacaag taaatggctg gctgcaagat gaagccgcca tctacggcaa agattattac
    15301 aatagataaa caggctaaat agttcgcctt gcacaaaaaa ccacggggat gcgaattccc
    15361 cgtggttgtt tttgctttgg agctggtgag gggatttgaa cccctgacct attgattacg
    15421 aatcaattgc gctaccactg cgctacacca gcaagattaa ttttgtgccg gatggcataa
    15481 gtattatagt ctaaaattaa ggtaaaatca agaggctaat tgttgccaac tattttgacc
    15541 tgcccccaca ccgccaattg atcatcctta atgagcagca ccccgctaac cccagggatt
    15601 tggcatgccc aggataaggc ctgctctaaa tcagctgctg actgcacccg attacctgcc
    15661 gccgtagcta cggcatcagc taaaatggta gaaggggaga taatcatggc ggcatcagct
    15721 ctgcctaaac ttaaggaagg gccaaccgta cccgatgatg tacagacacc aaaatcctga
    15781 ccctgtggtc tgatggccac cgcaaaacgg ttgcttaacg gtgaagtacc agcataaatg
    15841 cccactgtta cttcttccgt gacgtgaaaa tagatatcgc cgccgttttc cacgattact
    15901 tgcttcaccc gcggcagcag atcgtgtccc acatattcgg ctatcgcccc ggccaccgcg
    15961 gccatcggtc ccacacccac ctgattacca gccctagtca tggctaaaat cagcggcggc
    16021 tcctctgcgg ccactaggta tggctgcaaa gtggtctgaa actctggttc cctagcgatt
    16081 aactgctcca acgaagtacg ataataaagc acccgccgct ccacaaactt ctctaactgc
    16141 gggccatagc ttgcttcatc tacggccaca tacaaatcag tttccttgac ctggcaggta
    16201 aaggctctga aacctggtcg ctgcatgctg tggcgataca gtcgccgcga gctgtccatt
    16261 tttactctcc agccaaatat aaagcttcta ccgcccgtgc cgggcaagcc ttcagacagc
    16321 gttcgcagac tacacattgc tcactgcaaa aagaaactgt catttcgggc cgctgcatcc
    16381 acaaagcatt agtagggcaa atcactgtac aggcgccaca ctgtgtgcag cgatcttcta
    16441 accagacaat ctgctgctct aatggcgaaa cctctacgcc acgtgcctgc aaaaagtcca
    16501 gggcggtatt aaatccctct gcctctgctg tcagttctac gaccatattt ccctctttac
    16561 gtggattaat atttgctttg agaatattag ccaccacatt atagtctttc gccaaagtat
    16621 aaataaatgg ttgttctacc acagctgccg ggaaacgaag caccaatttt cttttcatct
    16681 tttcagctcc ttttactcta cttattcggc ttctacagta ggtaatgccg ctaccggttc
    16741 tgttagcgta aattcacctt tagtaatcca ttgtttgaga gtttgggcga tttcccgtgc
    16801 ccggggataa ctagacatgg gagcggtagg tacttccttg ccctgtacta caattgtccc
    16861 tgatttgagt tctgcatagc taacttcagc taaactgccg cccacacctt gggggtaagc
    16921 ttctccatag tccacaactt gggccataat atcttcatcg cgcactgctg catagctgag
    16981 cgtttctgca tctaaaatag gaataggcaa tcctacccct acctgcatgg aaacaccata
    17041 gcctaacaaa cttacccctc gcaaatagcg cgtgttcatt tgttttaagt cgccaatcag
    17101 agctagcgtg cctgccccgg cacgcggcac accgttggca ctgcggggag cattaggact
    17161 atgctgtgtt ccctgccagg ccacataacc gataccgcca cccaaaaaga gcttcgtccc
    17221 gatgcccagc gtgcgataat agggatcatt taaaagtggg gataattgtc cggcgctgga
    17281 gtagttggca ttacccatct gcggctttaa aatccccata taagtataga tagtccgctc
    17341 tgtcaagttt actgccacat tatagttttg gtaacagtta cgcggattaa acaaataggc
    17401 ttcgttcatt tccgccaagg tgatctgctt ctttaactgt tgacgggggt agcaatcagt
    17461 accgtaagag gaggcctcca gtagtacagg cttaccggcc actaaatctt ctatcacatg
    17521 ggcaccaccg taattaaact ctcctgggtg gctgctattg gcagggtcgg tctcgggaat
    17581 ctctgtcgcc cccagataga catccactgc cgccaatccg gcataggcat tcacaccatt
    17641 taggtgtact ttggtcatgc gcatccgcgg ggtggcgtgc cccacgttaa gcacagcacc
    17701 ggatgagcac atgggcccaa aggttcctgt cgtcactaca tcgacacgac gggctgtttc
    17761 ttctaccccg tgttcagcga ctaaggccaa aacttcttct gctgtcacta ccacagcctg
    17821 acctttggca attttggcat taatctcttt aatactacgt agttgtgtca tttagttccc
    17881 tcccctaagt aaattaaaac aaaaaatccc cttctgaaaa gaaagagata tcgcaaaatt
    17941 acgactctca tcttccagaa ttattctgct ggaattagca ccgtgactgc tgtccggttg
    18001 ccgggtttca ttgggccagt ccctccacct ctctggataa gagtgtgtat ttaattgtat
    18061 ctatcctata ctgagagcat ttactctgtc aaggaataaa tcacatcttt tctaaaaaaa
    18121 gtgactgccg cagctgctgg tgctgcggcg gtcactgcta ggagaaaata ctatgctgtt
    18181 cttacttttt tacaagctgc cagttcggcg gcaataattt caccgagaat cgctatacct
    18241 tgtttaatct tttctggtga agcctcagaa aatgagaaac gtgctgtctg cttaccatga
    18301 tcattaacat agaagccggc accatcgaca aaggcaactt tgcgttcaat tgctttcagc
    18361 aaaacttgcc gtgcagttaa gccgccgggg aaagaaaccc agataaagaa accaccttgc
    18421 ggctgtgtcc acgttacacc cttggggaaa tgctcggcca tacaagagag cattaagtca
    18481 cgacgatccc ggtagcagcc aatcagggaa gccacgtgct gatcaatata accactggca
    18541 gcaaaacggt aagccaagcg ttgaccaagc gagttggaac aaaggtcagt agagcccttg
    18601 gcgctgacaa ttttggccac gacatcttct tctgcaacca gccaaccgac gcggatgccg
    18661 ggacagaagg ttttagaaaa agaacctaaa tagatcacac gcccttctgt atccattgat
    18721 ttaatactgg gaattgcttc gccctcgtag cgcagctcac cataagggtt atcttccaaa
    18781 attagaaagt tatactctcg cgctaaagcc aagattgcct gccgccgctc taacgacatg
    18841 cagatgccag aggggttctg gaaattgggg actaagtaga tgaattttgg tgcacgcccc
    18901 tccatcttta gtctgcgcaa acgatttgcc aataaatcca tacgaatacc gtcatcatct
    18961 agcggaatgg gcacccgatc agcttcataa ttaaagattg cacccagacc accgacatac
    19021 ccgggctcct ccaccagtac cacatcacca gtgttcacaa aaagtttact taccagatcc
    19081 atgccctgtt gtgaaccatt cgtaataatc acctgttccg ccgtacaggg tgagccttca
    19141 cgtgtcattt tttcggcgac atactggcgt aacttgccat agccttctgt ggcgccatat
    19201 tgcagtgcca gcttcccttc ctcggcaatt agctcctgca ttacttctgt gaccttctcc
    19261 atggggaaaa agtcagcact agggaaccct ccggcaaaag acatcacctc tggctgttct
    19321 gttagtttaa aaaactctct cacggctgat gttttcatac actgggcacg gctggcatac
    19381 aactgttcga aattcatttg gcgcacctcc gtaatattta aaggaaatta ataagggcag
    19441 ttaaaaactc acttaaatac aaaaatcccg tctccgtaaa aaacagagac gggaatatac
    19501 ccgcggtacc actctggtta ccgaatcatt cggtcactca agctatgctt tctggataac
    19561 ggcagtgccg gcaccgctta ctatagttca gcgggcagct ccggggcggc tcacctttag
    19621 ctatagccga ggaacctttc agcttatggt tccctctctg tcggctgctt tgggcttctt
    19681 ccccatcgtc gcttttaatg tcagtatatg atgttgatcg taaattgtgt gcgaaaattt
    19741 ttgcctaact aatgctgcta attttaatta ggctttatta tagcagagag ttaaagataa
    19801 tgcaagggca attttatgcg attttttaca atgattttct tcttgcttac ccctgataac
    19861 ccaccagaaa ggcttgttga ttagcctcga ggaaacgctc cggcactacg gctgccagtg
    19921 cggccagaaa actcgccaag gaaaattcca gcgtgcgggc taaaacaccc agtaaagcca
    19981 cattagctgc tttgggctga ccacagtcac aagccgcctc cagggcatta aaagtatgga
    20041 gcgcacccaa ttgtcctgct gcggccaaag cctgcatcgc cgggataatt tgcgctgggt
    20101 actgctgccg tcccatgatc accggcatcg gcttaatttc ttggctgctg caaatcatcg
    20161 tgccgcctgt gcgcagataa ggcagcaggc gccacgcttc taatttttct aaagcaaaaa
    20221 catagtcagc ctggcccaat tcaattaagg gcgaatgcac tgctgcgccg aagcggacaa
    20281 aagtcagcac actaccacca cgctgcgcca tgccgtgtat ttccgaaacc ttgagttgat
    20341 agtgatgatc ttgaatgact tgcgccaaga ttttactggc caacacaatc ccctgtccac
    20401 caacaccacc cattaccaaa ctggttgttt tagacatcct gctcacctgc ctgacggatt
    20461 gcctcaaatt tgcatacctg cacacaaact ccgcagccca cacacaatgc agcatctatg
    20521 gtaatctttt tgccttcctg caccagtgca ggacagccta acttcataca ggcgccacaa
    20581 ctagtacact tctcctcaac aatatagtac acttctggca cacttttatt taacagggca
    20641 cacgcccggc gtacaatgac cactgccgct tcacctgccg ctatttcctc tctaattacg
    20701 gcatctaggg ccgccaaatc ataagcatcg acctcgcgta cccgctggac accgagcgct
    20761 cggcaaatag cagcaatatc taaggccacg gtgggctgtc cttgcagcgt agtccccgtg
    20821 ccgggatttt cctgatgacc agtcattgct gtgatgcgat tgtccaggac taaggttaaa
    20881 gtagtgcccc gattataaac cacatcaacg atgcccgtca tgccagagtg aaagaaagta
    20941 gaatcaccaa ttacggagac tactttccct tttaaatccg ggttgccttt ttcataaccc
    21001 aggccggccg aaatgctggc ccccatacaa atacaggaat ccatcgcttc taacggcggt
    21061 gaagcaccta aagtgtaaca gccaatatca ccacttacta agagcttttg cctgcgcaaa
    21121 gtatagaaaa cactacggtg cgggcagccg ggacagagta ccggcggacg aacaggagca
    21181 ggctcttcac gagaggctgc cactgccagc ggtaccgtca ggggctcacc aagtattttt
    21241 tccgcaatca gattaggaaa aatttctccc gtcaggggaa ataatgcttt gccctgcacc
    21301 gcaatcccca gcgctttaat ctgttcttca aaaaatggat ccagctcttc cacaacatac
    21361 agctcttcca ctccggccgc aaaggccgcg atcaactgcg ctggcagtgg gtagcagacg
    21421 cctaatttaa gcaccgaggc ctcaggcaat gcttcctgta cctgttgata agagataccg
    21481 gcacaaataa cgccaatttt ccgctgacgc cattccaacc gattgagtgg tgtctgttct
    21541 gcataatcac gcagccgctg cagacgctcc tcaactgcga catgtcgtgg tcgggcgttg
    21601 gctggcatcg cgacatgctt ctgcacattc ttctcataag gcttaatggc aatctcttgg
    21661 cgctcaccta gttccaccag tgattgcgcg tgagaaattc gcgtactggt tcttaacagt
    21721 accggtgtat cgaattcttc actcacccgc aacgcttccc gcacaaaatc ctttgcttcc
    21781 tggctgtcag caggttcaaa catgggcact ttggcaaagc gtgcatactg gcggctatct
    21841 tgttcgtttt gtgagctgtg catgccggga tcatcagccg ccaccaaaac caacccgcca
    21901 tttactccca ggtaggagaa agtcattaag ggatcagcag cgacattaac acccacatgt
    21961 ttcatggcaa ccaagacgcg agagccagcc agcgctgcgc cgatgccaat ttctaaagcc
    22021 actttttcat tactggacca ttgagcatca atctcctgat aggtggccaa gttttctaga
    22081 atctccgtac tcggcgtgcc agggtaggcg gccgcaaaat gtactccgcc ctcataagct
    22141 ccacgggcaa tagcttcatt gccgcttaat agtttcttca tgctcccacc tcctgctgtt
    22201 gtcgtttatc aattactcgt ttagctttgc cggcggtgcg ctccaatgat ttgggttcca
    22261 ccaatttcac acgcgcctgc accgttaaga cactggctaa gcgagaggta attttctgtt
    22321 ccactgcttc tagatcacga aaagaaccgg taaaacgatc acgattcaat tccactcgca
    22381 cttccaattc atctaaatag cgcttctttt tatcaacgat aattaaataa tgcggtgcca
    22441 cttcttcaat ttccattaag acactttcta tttgtgaagg gaagacatta actccgttca
    22501 caattaacat atcatcagtg cgccctgtaa cacggcgcat ccgagctgtc gtccggccac
    22561 aggcgcaaca ttcacgcgtg accacagaga tatcttttgt ccggtaacgg atcacaggga
    22621 agccttcttt ggtcagcggc gtaaacacta attcgccttc actgccctcg ggtaaaactt
    22681 ctcccgtttc cggattaatg acttccacat aaaaatgatc ctcactaata tgcagaccgt
    22741 tgcgtgcggc acattctcca gctacaccag gccccataac ttctgttaaa ccatagttgt
    22801 cggtagcacg caaattcaag cgttcttcaa tttcttcccg catctggtcg gtccaaggct
    22861 ccgaaccaaa catgccaata cgcagcggta aagtacgcgg atcaatgccc atttctgccg
    22921 ccacctcgcc caaatacaaa gcataagacg gtgtggcaat taaggtggtc acgccaaaat
    22981 cctgaattag cataatttgc ttctcggtat tacctaccga agcaggcaca acagtaaccc
    23041 ctactttttc cagcccgtaa tgcaagccaa agccgccggt aaataaaccg taagaaaaac
    23101 aaatttgcgc cacatctaaa ctggtaacac cggcctggac agcaatccga gccactaaat
    23161 ctgtccaagt agccaaatca tttttggtat acccagccac aatgggcttg cccgtcgtgc
    23221 cggatgaagc atgtacccgc acgatttctg ccatcggccg cgcaaacaag ccaaaggggt
    23281 agttatcacg caaagcatct ttgctggtga acgggaattt ttgtaagtca tccagcgact
    23341 gcaaatcagc aggatgtacc ccatggttgt caaaaagctc ccgatagaaa ggtacatttt
    23401 cataaacatt acttaccgta ctactgagtc gtgtgaactg caatgctcgc aacgcctcga
    23461 tactcattgt ttccgttttt gctccccaaa tcattttcct accccctgct actatgctac
    23521 aattttgtaa aacaagctgt ccctaacaaa aaacgacccc aaaaaaatgt gggccatttc
    23581 tgcacctaca atactaccgc cctacaaatt atataaagat atcatctggc acgtggaacg
    23641 aactgaccgt cagctcctac actagagagc gtatacttct tcacctgtta aaacacggat
    23701 attttttgcc cttaaagcct gcagggcagc ggcacaatct tccacgcgga aaattaccag
    23761 ggcctctgaa gaagcctgac caacaaaagc ataaagatac tcaatattaa tgcctgcctg
    23821 ccccatcgta tctaatactt cagctaaacc gccggggcga tccggtacat ccacagcaat
    23881 tacttctgtt tcgctaacca taaaaccgtc ctcacgcaaa atgttacttg ctgcttccgg
    23941 tttgttgact atcagccgca aaataccaaa atctgtggta tcggcaatag agagggcacg
    24001 aatattaatc cctttagcgc ctagagatct tgttacatct gccaagcgac ccgatttatt
    24061 ttctaaaaaa accgagatct gtttaacatt caccactatc cctccttcat cataaggtat
    24121 gtaaatttat tcgacaagaa gctcggcatt cctgcttgat cattttttta gctaatgcag
    24181 caaagacaca tttttttgac cactaagaaa acgctccgtg gcaaacacgg agcgttgctt
    24241 ggttactatc tatagggcag cgacagaaat caaatcacct atatattgga tgctttcttt
    24301 atactcattt ttttcaggct gtaagcggag cgctttgtca taccagtgcc aagataaacc
    24361 tagttcgccc aatttataac agacatatcc cagtgctgcc gagcaggctg ccctttccgg
    24421 ttccaagcgc caggcctgct ctagataggg cagagcctgt tctacttgac catactgaca
    24481 atgggcaacg cctaagttta aaaaaagcgg cccatactct gtgcaattac gtactgcttg
    24541 ttcataagcc caaatagcag cttcgtattc actgatttta acccagcaat tgcctaaatt
    24601 aaagaaggcg ggccaacaat ccggatcaca aacaactgcc gaaagtaagt gaaaggaagc
    24661 ctcacgaaac ctacctcttt gcatcattag tgccccttgg cggttataag cggcggcggt
    24721 acttttttta tcccctgaat gtggaagacg catcaattaa agaccccctg gcggcaaatt
    24781 tttccttata gtgaagtata agcaccaagt tgtccaatta tgcatcagcc accagatttt
    24841 aacctccggt tggataaagc agagcacacc tcgtggtgtg ctctgcccct tttacactgc
    24901 tattatcaag cgttagctat tagaaatatt tttctggttc agtcagaaaa gcgaatttag
    24961 ctggcgcttt aagaaactct aaatacatgg gcagaccacg aaccaaacca aattctggtt
    25021 gatccactac cggtcgcgta ctaaaacgcg gtgtgggctg atacttggta ttggccacaa
    25081 actgttggtc ttctccctca gcggtaactt caaaaaaggc gccacccgct aaatctttaa
    25141 taggctgata aacggaagaa aaatcagcgg ctacccagtt gctcataatt aaaaaggctg
    25201 agccggggtt aatggtaata tgcccgtagc ccgggggaat taagacttta tcgccggcgc
    25261 tagcttccac gagcagggaa tcaactccat cttcatccgg ctctgtttgc aaaagatagt
    25321 gtgcctgacc ggccagcact tcgtacactt ccggataggt agcttccgta cccggcttga
    25381 ggggatgata atggccggca gtctttatat attctctgcc aatctgtccg ggtctaatga
    25441 cagttacatc ataacgcagg ccatgtttag ccaacagctc acgatcctgc tggcgacaaa
    25501 gatcacgata catataatag agctcttctg gcgcttctgc ctggggatcc agcaagactt
    25561 ccagcatctc ctttttgcga cgtacagcgg gagtaactgc tgctaaatca gggccaaagg
    25621 taagcttgcc tgctttgtca gctgtcaaag gtaagccgct cgttttctgt aagtcaatca
    25681 tctatttgct cactccttta attatcccaa taacgtggct tgcacttcag cctggatggc
    25741 ttctttttct gctgctgtgg ctgcttctgt ataaatacga aacactggtt ccgtgccaga
    25801 gggacgtact aaacaccaac tgccgttgac cagcacaact tttacaccat caaggcgatt
    25861 aagcaaggcc accttaatac cagcaatagt ctgcggctgc caggctgcca ttttatcaat
    25921 gatggctgct ttggccgtga ccgtggtgcg cacatcaaga cgtgccgtgt agagcgggcc
    25981 aaaacgtctt tcaagatcag ttaataactg tgctgccgtt ttgcctgttt tggctaacat
    26041 ttccagaaag agcagggccg caaaaatacc gtctttttcg ggaagatgct tgttgatact
    26101 aacaccgccg ctttcttcgc cacctaaaaa agcattcttt tcacgcaaag cctgaccaat
    26161 atatttaaag cctaccggag tttcgatcac aggacaccca tagcttgctg ccacgcgatc
    26221 taacatgtga gtcgtggcca ctgtacgagc caccgccccg cgaaagcccc gcgcctttac
    26281 tagatattcc ataaataaag ttaaaatttc attggggcta taaaaacggc cctcaggatc
    26341 gaccacacct aaacgatcgg catcaccatc taaagccaca ccaaggtcgg caccgctttc
    26401 ttgtactcgg gccgctaact cctgtaaatg ttccgcggca ggatcgggca gcgaaccgcc
    26461 aaaaagcgga tctcgccaat tatgaatagc ttctacggca cagccatgct tcgctaaaat
    26521 actttccaca taacccatcc cagcaccgta catggggtca actactgtct taagaccggc
    26581 ttttttaatc gcggcaaaat ccagcagact ctccaaatgt tccgtataga caggtaatgg
    26641 ttcaatctct gtcactaacc cttgggcgct ggctgcttca cttgccaacc gctgcactct
    26701 gccgctggag aggatcgttt gcaagttagc cacaatcggt ttgatttctt ccggcagcgc
    26761 cggtccggca tagtccggaa taaatttaat acccaggtac tctgctgggt tatgactggc
    26821 tgtcaacatc acggccccac ccgcttggtg agttaagacc gcatgcgcca ccgccggtgt
    26881 tggtgtatgt ttgatacaaa gataagtatg aatgccgtta cccgtcatca cctctgtcac
    26941 cacttgcgca aactctgcgg ccagaaagcg ggcatcataa ccgaccacga caccgtgcgc
    27001 tgctaaacct tccttttgta gatgttgagc aatagcttgt gctacataac taacattagt
    27061 aaaagtaaaa tcctcggcaa taattgcccg ccaaccatcc gtgccaaaag taatttctgc
    27121 caccctcgga cccccttaga caacattggt tcaggctgtc gggcagcctg aacccaaggc
    27181 ttgtcaagat attgtacctc atatatattc atgattttat gcagaaatcc tgctaggtgg
    27241 aagtttaatc cagttttctg cttctacagt tcctgctgtg ccaccaagac cgccaaatct
    27301 gccacgcgat gtgagtagcc ccattcatta tcataccagc taaccacccg gattaaattt
    27361 tccccaatca ccatggtcag caagctgtct accgtactgg aatagggaga acctttgtaa
    27421 tcaatggaga ccagcggttc ctcactgtaa gctaaaatac cctgcagttc cccggcagcc
    27481 gcggccttaa aggcttgatt aatttgtgcg ggtgtggcag acctttccag ctcggcaaca
    27541 aagtcaacaa tagatactgt cggtgtgggc acacgcacag caaaaccatc aattttacct
    27601 gctagttcag gcataatctt acctattatt ttggccgcac cggtagtggt aggaatcatc
    27661 gacaactgcg ctgctctggc ccggcgcaaa tcggggtgcg gcaaatcaag taaacgctga
    27721 tcattagtgt aagagtgcgt ggtattcatg aggcctttac ggatgccgaa gttctcctgc
    27781 agcactttaa ccaccggtgc taaagcgttg gtagtacaag aggcgttaga aataatctga
    27841 tgtttgtccg gatcgtactg ctgctggttt aaacctaaga ccataataat atcagcatca
    27901 ttgacaggag tggtcacaat tacttttttc gcccctcctt gtaaatggcg ggaggcctca
    27961 gcatgggtac gaaattttcc cgtggcctcc agcaccacct ctacccctaa ctcacgccac
    28021 ggtagttttt ccggcagtgc ttctgccaag atcttcactt cacgtccctc tacccgtaaa
    28081 ttatctccct ctaaggccac ttcataaggg aacttaccat acatggaatc atatttcagt
    28141 aaatgagcca gcgtcgcatt gtctgttaag tcattaaccg ctgctatttg cactgtctga
    28201 ttgccctgca gggcacgaaa agtattgcgg ccgatacggc caaaaccatt aatgccaatc
    28261 tttacactca ctatgtaccc tccttaccgt caccgagttc tcaactctag acaaaagata
    28321 cttgattgtt actttagctt tccccgagat tggcatccaa tgcgacacac ttgacaatcg
    28381 acaaacgata taagacaatg caaataaagt ggttagcatt ggggcttgct ttggcgtcag
    28441 tcaggtaagt cctaaatacc agcctaggag gaattatgca tgacgatcaa agtagctgtg
    28501 aacggtgccc tcggcaatat gggcaaaact gtggtacgtg ccgttcatgc tgaccccgca
    28561 ttacaacttg tcgctgccat tgatgtcaat ggtactgaca atgatattgg tcccattgct
    28621 ggtatcggag aagtgaatat tcctgtctcc accgctttgg ccgccactct gcagcaaacc
    28681 aaacctgatg tggtagttga ctttacttca ccctttaccg taatgcaaaa tattgccaca
    28741 gccctttctc tgcaggtccg ccctgtcgtg ggtacgaccg gcatcactga agctgaccta
    28801 gaaaaaatcg ctaacttatc ccttcaatac cagacatcag cagtagttgc gcctaatttt
    28861 gcccttggtg ccgtcctcat gatgcagttt gcggcccaag cagctaaatt ttttcccgag
    28921 gcggaaataa ttgagctgca tcatgaacga aaaatcgatg ccccttctgg caccgccctt
    28981 aaaacagcag aaatgatgct caaagcccgc ggacaagctg tcccccggca agttgaggaa
    29041 ttagaaaagt tagctggcgc acgcggcggc aacctccaag gcatccgtct acatagtgtc
    29101 cgtttaccag gatatgtcgc gcaccaagag gtaattttcg gcggtctcgg tcaaacctta
    29161 accattcgcc atgatagtat tgaccgcact tcctttatgc ctggtgtcct gttaagtatc
    29221 aaagaagtaa tgcagcgcga aggcgtaatt tacggtctgg aaaatatcct caatttctaa
    29281 ttccactccg gacaggcacg aacccccctt tttctgcata tacattactg cagatgaggg
    29341 gggttctttt atgcttttag aaggaaaaca aatcggtttc gctttacctg aaggccattt
    29401 ttctatgcct gctattctag atgaagtccg taagttagtg gctgcaggtg ccaacattta
    29461 tccactcttt ctggccacct ctgaccatcc cgaattggag ccgcactatc accagaccaa
    29521 gcagatgcta gaacaaatta ccggacacga aatgatgccc actactttag ctgaagacgt
    29581 cggtgaatta tttagtgagc aattagcctt tgacatcttg gtggttgccc cctgccctgg
    29641 aaatttttta gccaaactag ttaattcgcg cacaacctct atttctttgg ctaaatccgt
    29701 taaacatttg caggtaggta aacctgtggt cttagcgcta attaccaatg gtgattcaga
    29761 aaacttactc caaaatgtgc agcagatttt aagtttaaaa tctttcttcc tcgtgccctt
    29821 tggtcccaca gatgaagacg gtaaacaaat atatctctcc cgccttgatc tgctctacga
    29881 aacagtactg caagcgctgc agcaaaagca actcacacct gtctacatcg agccctgctg
    29941 gctgcccagt taagctccaa ggcacaaaaa aaaacaataa gcaaagcctg ccgcgtaaaa
    30001 ccatcctcgg caggctttgc ttaaagcagt ctgctttcta aatcatcaaa ataaaagctc
    30061 accaaacggt taatcatggc tgccatcttt tgtgcatcct gctgctggcg cataataata
    30121 aaaatacctt cgataaatcc attcaccaat acatagataa ctgtctcatc tgcttcgcta
    30181 ggctggcgga aaattttact tttgatccgc tcacaaacca tctctaacag cgattcttta
    30241 aaatgctcat atttggagcc ttcactttta tcactgatta ccagcagctc gcggccatag
    30301 gctaaataaa tcttcatgat gccttctgca atctgctgcg ccgtggactt aacgttgctg
    30361 ccgttttttt cactttcgtt acgcacactt aaaatcaaag ccgacacctg tgtatgtact
    30421 gtgcgtacaa tttccgcaaa aatttcttct ttaccgtcaa agtaacgata aatattactg
    30481 gtagaaatat cagcaccctg agcaatacgc cgcatctggg agcgtgcaaa accatgctcc
    30541 gcaaattcag ccagggcact acttaagatt tttcttctta tttctggttt taaatattgc
    30601 atcgataaaa cccctaaccc ttgtttctta actttatttt atagtaactt atggccttga
    30661 gcaattaatt ttggcaagca aaatactttc tgtaatgctt gcacatagct gtactgtttt
    30721 tatttaaaat acagccctgt cgtcttactt cctattttta gtgaggttat taataattaa
    30781 agcactgacc acgatcacag ccacatacca actgacagtt aatacgctgt cctgtctgcg
    30841 cagacgcgcc actttagtca atttttctgc cgcggttagc tgcgccgctc tctctatacc
    30901 aaccggcact cggattagac cgccccacgt atatgtttta acccattcaa tgcctgtcca
    30961 acggtcacga tagtgttgtt taatgggcgt ataggcaacc tgttgctgac gaaagataaa
    31021 ccctagtact agtgcagcta gtacaaaaac aagtaagcat aaccctagac gctgtcgcaa
    31081 caagaccacc cccagcctat cttcattata cacaataatg gaaatatttg caattattat
    31141 ttgactaata agctgtcata aattgactaa aaacttctta cataacagtc tgcaaccctg
    31201 gttaagatac tagcgtaggg ttcgtaatgg ccgagtcaag attactacag ggcttaacgg
    31261 ttctctagta gtcggccaaa ggtctgcatc gggtcaagtt ggttcggtgc ctggccggcg
    31321 ggaagatctg cagtcttctg caaggatgtc tgtgggtcga gctaagatag tcacaggtgc
    31381 taaacatccc tcttgtgtac tgaatatgca ggagctgccc acgacctgaa acggttaggg
    31441 aggattagta agtatgtgtg tctgttgagc taaaggttgg ttgaggttgc agacaactac
    31501 ttgagttgct aaacttaatt agttcgttac tgggatctca gtcagccggc gcgcaggtca
    31561 ttacgggctc tacaccttta attaaaggtt catcaataag cttgccgtcg ggcaagtttt
    31621 tttatggtga tggtcaggca gagttatttt tgaccaattc cctacatttt tgttataata
    31681 ggcttggtaa agtaagcgcc catgcagcga acagtttgcc acgtttcgct gccaggtctt
    31741 aattaaaaga taagcaagaa agatagtaca gcatgatcgg aggaggctat atgaataaga
    31801 acccttataa catggctgtc accttggagc tggcgcaaga gtcatttgcc caaagaaacc
    31861 cccagacgat ggccaaattt agtgattgtc tttatgatga aactacgcac tgcttcacag
    31921 ttccttactt aggacaagac tataccgtca gctatcctgc aggagaggtt accgatgccg
    31981 cccacaaaga agttgacctg atcaccgcta ttctgctggt gcactactta accggagcta
    32041 gcggcgtagc attaacaaat aactggatct cctttaaaga gttacaaggg ggaagcattt
    32101 atattgaccc ctttcacaag agagctattc agcccatgct gaaaggcttt ggcacacagc
    32161 cggcggcctt cacgagaacg gcggcgcttt taggtgggca gccagcctct tttggtgatc
    32221 tttcctatcg cttccctgta ctgccgcgca tccctattat cctcattctc tggcagggcg
    32281 atgatgaatt cccccccaat ggcaacattc tctttgatca acatgccaat acatatttgc
    32341 atactgaaga ttatgctcat ttagccagtc aaacagttta cgccttacta aaaaaaattt
    32401 aaagtaattc cgtcagattt cacagtcatc ggacaatgat tgccgagata atctgtatat
    32461 aaagactaaa aaaactgcca tctggcagtt ttttgtctag gaactgtttt ttatgtccta
    32521 cgctaaacgc atatttcatg ggacttcgca ctttccctgg gttacaatca ggagtgaaag
    32581 tgaggaatcg ctatgtttaa atggtctacc tccaccgcag atcaagaacc gctgcgcctt
    32641 aataatgcgt tgttgtggac tttaatgctc atcgtcgtca gtgcgttggg ccttggactc
    32701 tttccctggc ttactttctt agcgacactt catcagtacc agcagtcttt aatgctttgg
    32761 gggttataaa atgaaacatc tcaccctaac gcaacgcaaa acactgctgc tggtgctcct
    32821 tgctctgtgg ctgatgatgc tggtatacgg cattattgct atttcctccc tttggtctcg
    32881 ttaccaactt tttaacgtgg ctagtggcag catggaacca gctttgtcca ccagcacaat
    32941 tatcgccgta gccaaaaata atggtacttt atacgctcct ggtgacatta ttacttacaa
    33001 acaagatggt atatacatta cgcatcgcat cattgagctt ggttataata agggttttta
    33061 ctatcgcaca caaggtgatg ctaatcaaga tcctgatcca ggcttaatta agcatcaaga
    33121 aatatttggt caggtggtct tcactgtacc tgcattcttt acccccttac gccggatact
    33181 caatgtccgc agcctgctgt tatttccggt gctgcttttt gcagcattat atctgaaaaa
    33241 aaaccgcaaa gtggcggcac gtcgtccgct ccataattag gtaataagct ttttccccgc
    33301 tccaccaccc tgcacggggt ggttcttttt ttgctgcgaa aagaaataat cccggctgcg
    33361 ccgggattat gctgctttat tttagttttg ccttgggcac atcttcacca gtcagtcctt
    33421 tgtacgactg ttgtaagagc tgcacagtca aagtggggtt atggatacct aaactgccgt
    33481 cactgctgat taaaagatag ttataaccag cttgatacat ttccttaggc acctcgtcta
    33541 aagcggcacc ctcagcatct ttaaaaacta ctgctccatg accgctctca aaagaaccgc
    33601 cagctaattc ttccccgaca gctgcctgca ggagtgctaa taagccttcc acttctgttt
    33661 ggaaaccttc ctcctgaccg tcaccatcat agtcaccagc cgcagttaaa ttaagatcat
    33721 tgatatcttg atggcattgg ccacaggccg cttctacctt gtcaacttta aagctatggg
    33781 aggcatagcc gccttctgtc tccagcagat gacaacctac acagctatca tcaatattga
    33841 catgggcact cgtattacca tagtcaaatc cctctaaacg catgccacca gtgccggtgg
    33901 caagatcagc ctgactgcta tagtgcggtg ccgagcggtt ctcatcctca atatttgggg
    33961 ctgtacggga gttatgacaa aacatgcaga ccgcccctga accgccttgc accttctccg
    34021 ctgtagggat cgttactttt ccttcctcca ttaggtctgc gccctgccct gtatgacaag
    34081 cacggcagtc cacactaata aagaaatccc gctcaatagc cgccacttca gttatatttt
    34141 ccgcaaaagc accaccatca tggcagacga cgcactcatc gcgctgggca ggagacaaaa
    34201 gaatattact atgtttagat tccacccatt gggcggttaa ggccttaata tctacattat
    34261 caccagcttg cttcttgctg ccgtcctgtg atgttgaatc ctgctccttg gtgtctccgc
    34321 cacagccggc agcaatgagc atggacaaca gaatgagcaa ccccaaggtt agccatttct
    34381 tcgccttcat tcagccaact cctttcagct tccttactgc atatctttac caagcacttt
    34441 agctttatgt atctagcttc taataaaatg ctgcctagct gtaaggatag ccggaggagc
    34501 tcttgggctg catcattgtt tgcgccgccg gcggattagt cctaaggctg cctgcaattc
    34561 ttctactttc agcagataaa ttaagacaaa atacacacct gcgccaaggg cgattaagat
    34621 acccacttct aatgcttgtc gcataaagct aacaccactt aaaaagcgtg tgccatacca
    34681 gacgagcagc cccatcaatg ctgaagctaa accagtcttc caaaaagtag agtagagctt
    34741 gcggccgcca aaggcaccaa tcttgcgcca cattaaatat gtcatgccca cagcacccac
    34801 ataaatggaa gttgccgtac cgagggccaa gccaccatgc gccaatggct tcatcagcag
    34861 ccagttaagc actgtattaa caatcatggt cacaaaagta aataacatcg gtgtcaaggt
    34921 atctttagta gcataaaaag ctcggctcat taagagcatc acccctaagg gcagcagacc
    34981 tagactataa aaggccaggg caaaagccgt attttgtgtt gctgcagcat caaagacacc
    35041 acgctcaaaa agcaagcgaa caatgggtac ccgcaaaata atcatgccca ccatgatggg
    35101 gaaaatcaag gtaataatta agccgatgcc atggtgtaga gtatacttaa aatcatcaat
    35161 tttatcttct gcaaataact ccaccagggc tgggtacagc accgtgagga gagctaaaat
    35221 aataatgcca ttgggcaata aataaattcg gttagcataa ttaagcgccg caatactacc
    35281 ggttgacagc aaggaagcaa atatacggtc aatgacactt tttaattctg tggctgcact
    35341 gccaatgatc accggcggca ataaaatcag tatcttttta atcccgggat gatgccactg
    35401 aaaatttgcc cgctgaaaaa caccatgact ggctactgca ggcacttgaa cgagaaactg
    35461 actggcaaag ccggccattg taccccaggc caaccccact atcccgtagc gtgcaccaaa
    35521 tatgatggcc gtcaaaataa tcaacagatt actggggtaa gcagtaatag ccggaataaa
    35581 aaagttcttt ttaatatgca ggataccgct ggctaaacca gttagcccca taaataataa
    35641 gcccggcagc ataatcctcg ccagtacaat ggtgaggtct ttctgtgccc cctcaaaacc
    35701 tggtgccagc agctttacca ctaacggcgc tgctgccacc aaaagcatgc ttaccaccac
    35761 taagatggcg cctaacagga cgaaaacagt ttgtgccaag gcgttaccct cttctgtacg
    35821 gtgatattct ccgtacacag gaataaacgc ggttttcacc gcgtcattaa acgccaaaaa
    35881 taaaattaag ggcagcgttg acgccattaa ataagcatct gcttgtgcgg ttgtcccgaa
    35941 acgataagca atggccgatt cccgcagaaa acctaataaa cgggagatta ccaataatac
    36001 ggttaccatg ccggctgatt ttattaacga actttgtttt gacatgacac tcttccttct
    36061 cccagccctc attttagata ataacagtgt acacatgtac cgtttagcat agcttataaa
    36121 ttgtaaactg ccctcattca attctaatta aacacatcga ttaccaaata agagttgaat
    36181 aagatcacaa agcctgccgc cactacacct aaggcgataa cccaaaaggc ttcgcgcttg
    36241 ggcacacgga aaaaacgcgc agccacacag gcagtccagg caccggttgt cggcagggga
    36301 atcgccacaa acaaaaacaa ggcaaacttg ccatattttc gaaacatgtc cttcttttta
    36361 ctaaccttat tgatcagcat tctatagacc ggtgctgtta ctgtgttttt ggctaaaaac
    36421 tcctctaaac gctccagcac ccataaccat ggatagacaa tcagcatatt accggctaaa
    36481 ctagcaaaaa aagcttgcca taaggtcagc cccagatata gccccaaggg aatgccgccc
    36541 cttagctcca ctaccggaag cgctgaagtt actaacactt tcaagtatgt cacatcgatt
    36601 ctcctccaaa tagcttagct atatcatttc gctacattcc ggcaaaatcc tgccgcccaa
    36661 tcattgccca gctgctgtta tgtttttcaa ttgtctcggc aactactcgc acgagacaag
    36721 aaatcttttt gctgcttgca gcgtgctctt aattgtaacc cgtataaaaa aaaactccgc
    36781 gagggagctt tttctagcga atcattagcg aaccacttga atccaagtgt tgcctaaggt
    36841 taaatctact tcagtaccat ctgctaattt atatactgtc tgctggccac tcttctccca
    36901 ggttaaatca tatttttgcc cgccctcata gagcaccgcc gggcctgaac cgctgacgtc
    36961 aatatctacc aagctggttc ctgccacatt aacatggacg ttggtacgta caatcacacg
    37021 cgtggcacca tactgcaacc ctgtttcccg atctacatgg gcggccccat cttcaaagcg
    37081 catatactgc tttttcacgc tatcatattt gtagctggcc gcattattgc gattatagcg
    37141 cacttcaaat tctaaagcct cttctcctgc gggcagttcc tggggcataa ccttgacact
    37201 tttggccatt gacgcccccc tggctgcata caccttttcc agatttgtat acaaattgtg
    37261 gggtgcttta cggctgtcat cacgccagaa atacttgcca ttagtaatct tctgcaggtc
    37321 taaatgagca atcatttcat ctgttcggcc agaggtgcca gaaaaagcaa ccgccgcacc
    37381 gtgctccata gcggtttccg caaagtaagg gcgtaaactg cgcactggac caatggtata
    37441 attttcagtt aaaggctgat cgaagacagc gataaaacga gtaatgccgc cttctactaa
    37501 aaactcgtag acgagatgtg cctgctgcat gccggattgc ggacgagcgg ccttatggtt
    37561 attaatcaca acacaaacag ggtccaccaa ctctactgtt acactgccat catccggcgg
    37621 cgctactggt tcctgttcca ccgggtctgg actctctttg ccgcaccctg tgactaccaa
    37681 tgctagtatc aacactagta agaaaaatcg taatgagctt ttcatcatta tcttccccct
    37741 tgaggttgtc catcatttgc tactaattta tgccgacctg gcgaaaatag tacaactttc
    37801 ttgagccaca acctctgacc ctattatatt atatttaagt gcgataatca tatttatttt
    37861 aagcacgctg ataaaaatag tgctcattac cttagttaaa gtttgagcac tggctactga
    37921 ctaaacttat tttttattta taggaaatat catgccgttt tgtgctctta gacagggagt
    37981 atgatgccca cacgattacc attgtgcagc aggagctaat ttgcggcgat agtgtacccg
    38041 gcaggtagtc tctaataatg ctccaataaa aaagtgttga caggggcagt gatagccgtt
    38101 actataaatg ttagtactca ctaacattct atattacggg agggaacaac tgtggctaac
    38161 ctatttacag gcctaagcag gcaaatagtg aaaaaacctg tgctgacact ttttatggcg
    38221 atcctgatca tcgccttgtt gttagtaggg gcactgaaaa tatctatggc taccggtacg
    38281 gaaactttta tcgacacgga aagtcaaact taccaagata atgcttattt ggaggaaaca
    38341 tttggtggtg aaaatattgt tgttttgttg acagcagaga atttagatac cttgttaacc
    38401 gcagagaatc tggctaaact ggctgctgta gaacagacct tcacacacag tgacagtgtt
    38461 tatacagtga ttagtcctgc gacaatggtc aaagaaatga ccactaaaca agcagctaca
    38521 atgttggcag gcgtaagtga gatgaaggac ggcttgacaa caatgagttc gcgcttgctg
    38581 gacgtggcgg caagtatacg taaaatgaaa gccgagatgc caaacgtaga tttcagcaaa
    38641 acggcagcaa gttttcaaga gcttacttcg ctgctggata acctgattac tggtcaaaag
    38701 caactcccaa caggtattgc cgaattgggt agcggctaca accagtttgg tacacttgcc
    38761 gccaagacag gcacacaaat caagcttttc agtggacagt tcgcgcaaat gttagagacc
    38821 ttaccgctgg aagatggcaa gaaaaatgaa ctgcgtcagc agacagcagg cttagcgcaa
    38881 gctggcgaaa tgctcacaga ggctggcagc aagatgacac aaataagctg gcaggcaggg
    38941 agtatggccg aagcaccact gcaaacagcg cagggtttag aaaaaatgaa aaatggcctg
    39001 cgcacacaag tgaaaagttt gcagcagctg cagcagcaga tgcctgactt atcccaatta
    39061 gatacattag cggcggcgat ggatactttt tcgcaaaacc tgttaactat tgcccaaggg
    39121 ctggaaacaa tgctggcgca aagcaatatt atgtaccctg gcttaccccg cagtcagcag
    39181 actttagagc tgctgcttta tgaagaaagc ggcgaacttc gtccgctttt caaccagacg
    39241 atcgttgatc agcagcacgc gctgatgatg attaaactgc aaggccagct cagtgatgcg
    39301 gagaaagaag aagtcgtaga gcagcttaat aatgccttga cgagaaataa gctgacgtct
    39361 gttaaaacaa ctgttactgg taagccggtc ttagatatgg tcttacgcag tgaaatgcgc
    39421 caaagcatgc aaaagatggt ggtcctagct gtgctgttga tggttgccat cattacgctg
    39481 atctttaatg tgcgttggcg gttgtttccc ctcgtggtta acttcattgc tgttttagcg
    39541 accatgggtt tgctggggca tctacagctc ccgatgacga tggtttccat ggcagccttc
    39601 ccaattttaa ttggtctggg cattgattac tccattcagt tccacagcag atatgaagag
    39661 gaatattggc aggaggcgag cagtaatgaa ctttaaacga gtcatcacta aaacatatca
    39721 aagtatgggg ccggcagttt tgattgccat gatcgccaca attttaggtt ttgtcgcctt
    39781 gcttatttct ccagtgccca tgattgccga ctttggcaaa atgctggccc taggtttagt
    39841 gctcagcttt ttggccgcgc tgatcatcct taccgtcagt ctgtatatta aagataaatt
    39901 ctttagtcca gctgcgggtc agaaaaacta tcaagcagct cccgtggata ggcttgagca
    39961 gccgttagcg gcagttacac ggcaggtact gcgctataaa tttgtgatta tcctgttagc
    40021 gctgctaatt acccttggcg gtttatacgg caatagcaaa gtaggtgtgc aaactgatat
    40081 tgagcagttt atgccgcagg atacacctga attacaggaa attagagagc tgcgggaagt
    40141 aatcggctcc acagagcaag taacgattat gctgcagagt gagcagctgc tgacaaccaa
    40201 gaatattgag tggattgata caattacgca ggagctgccc acagcttttc ctgaagccat
    40261 tgttgatgtt agatcgatta cttctgtcta ccgggaacta aatacggatg aatttgcagc
    40321 tcaacaggta gccggtttca tcgaccagct gccggcggcg caaagaaaaa tgctcattga
    40381 cgagagtgag cgagcgagtg ccattaccgt taatctggca cggttagaaa caagcaagca
    40441 ggagagtttt attacgcagt tggcagaata tttgtcagtc gataaccctg cagagataga
    40501 tgtaacgatt accgggcagg cagtcattga cgtacaaatg atgacggccc tgacgagcgg
    40561 tcgtactgaa atgactctcc tggggatggg gctagtattt ttggggctgc tggttattta
    40621 ccgcagtttt cttaaagccc taattccgat cctgcccatt agtctgattg tcggttggtc
    40681 aggtggtgcc atgtatctgc taggttttaa ttacaccccg ctgacagcaa cgatgggcgc
    40741 cttaatcatt gggattggta cggagtttac cattatcctc atgcaaagat attttgaaga
    40801 aaaaaataaa ggcctgtcca aggatgaagc aatgatcaaa actgtgggca aaataggtaa
    40861 accaattctg gcctcagctt taactaccat tgccggtttt agtgccctaa tattctcgga
    40921 ttttctcatc ttaagagagt tcggcatcgt gactttacta aatatcattt tctgcctctt
    40981 gagctcactg gtagttttac cacctctgat tgtgctttta gacaggaagc cagttgcgca
    41041 gaaagcataa ttaccgtgtt tgacgcgttc tctatttaat aagtcaacag gcgcagcgcc
    41101 ctcagcgctg cgcctgttgt ttgcatttaa aggttaaaaa actagctctg accctaaggc
    41161 agtaatgata ttttgctgct tgaatttagc actggcctga gccatagtga gaaaggtgac
    41221 aagaaagacc ccaaggatac aaataataat ttcctgccag gggatgatga aagtaaaggt
    41281 aaacaagttg ctaaaggcat taaacatcca gaaactgatt aagatgctca ggggcagacc
    41341 atacagcagt gccttgaagc cataaaaaag actttcaaag cgaataatct tctgtaatcc
    41401 ctgtggcgtt aaacctaccg actgcagcat ggcaaactcc cggcgacgta aggccacatt
    41461 ggtactgatg gtattaaaaa tattagttac accaattaaa gtaatcaagg tcacaaaacc
    41521 gtataagaag atagccatca cggtcgtggt ccgtttcatt tctgcttgca tttgcgagat
    41581 atcctgcata taaaaatcag cctcgacttg ctcttccagc actttaattt tctctaccaa
    41641 ggcgacacta tcagagctgc tgagatacag agcagcttgc tgcgcctgca gctgactttc
    41701 tgcaggcagc tgtgcagcga tagcggtaaa gacctcgtct gaaaccacta tttttaaccc
    41761 catctctgtg aaattgacgc caaagggcag ctcctcactc agggcaccaa tggttagatc
    41821 aaactcggcc ggcagagaat ctgcctcgta ttgacgatcg acaaaggaca gctctgtacc
    41881 agctttgacc ttcaatggtt gataggatac cttaccgtcc agcacatttt tattgagcaa
    41941 aatacccttt agccgctgcg ggtcctgata atcttctggt tttaaaccaa gctctgcggc
    42001 atacgcggta aattcagcct ctccaatggc cacaagctcg acattaagct gatactgccc
    42061 ctgctcgttt tgggggaaat attctgcttg caggaaattc ttttgcagat aatcaccgag
    42121 ctgttcagct gccagctttg tctggctgta aagcgtacgg gccacagctg ctctttccac
    42181 ctctggcaag tccagcactt gctgataaaa ctgattttgt ccctcgctgt cctccccgta
    42241 gtcactgctg acaaccacat tataggcgat gtctccatag tagaggccac tactaataaa
    42301 accatactcc ataaagctgg aaaaggtcac aaagagcaca atactaatca ccagtgagaa
    42361 gacagtagcg cgatactgtt tacggtttct tttcatattt tttaatgcca gttctccctc
    42421 aactcctagg agcttgctgg ccagcttatt agtttttact tctttgccct taagcttcag
    42481 gtcgtctgtt aagcgaatcg cctcgatggg agaaattctg gccgctcttt tggccggcag
    42541 ccaggctgat aacaagatgg ttagcgcttc aaaggcaatg gtaatcagga tagtattagg
    42601 cgagatgact aaacgtaaag ccagctcttc actaaacata ctgcccatca ttaactggtt
    42661 aaccaccttc agcgtaacac caatgccgcc cagcccggca ataatgccca gcggaatgcc
    42721 gattaaacct aacagtaggg cttcataaaa tactgtactt tgaatttgtt tcgccgtggc
    42781 gccgacactg gctagcatgc caaactgctt tttacgttca ctgaccgaaa tggcaaaggc
    42841 attataaatc acagccacag agccgaccac aattaaagca ataataatta aggtcaggga
    42901 ggataaaaaa ttcatcgtgt ttctattgcc agaaacacca gaccaacgca gcaattcatt
    42961 gttataagta aagtcttgcg caccagcatt ttgcgccatg actggtactt cctgaaagat
    43021 ctgcttaagc ttattgctga gcatagaaac attaacggta tctaattctt gtagtgcagc
    43081 tgcatctaaa tgagcaaaaa cagtatagcc agggctaaag tcaaaaagcg gatcagcagc
    43141 tactcccgcg attgtaaagc tttgctcact ctccggttga aatgtttctt cagcttgata
    43201 attagtatct gagcctaact gctctccgtc taataacctt tgcccgaaag ctaaagtgat
    43261 cgtctctccc agctgatagc tgccctgctt ttgtacctct tctgggataa gcagttcatg
    43321 actagtcgcc ggcaatcgcc ctgccactaa agtagtcggg aaatgcgcaa aggctgctcg
    43381 gtcatactgt ttgagcagta aataaggttt gtactcgtca gtactctgcg caaaaatcgc
    43441 atagcccttg ctgcggctca gcattacggc tgcggtagaa tgatctttag cgataatatc
    43501 cactttatct aaatcgacat cataaaatga agcgtgataa ttaccgcttg actgaatagc
    43561 tgattggata aacaaatcct gaaaactagc cgcaatactt gctacaccca taatcatggc
    43621 gccggaaaga ataataccaa tcattgtcac aatagtccgc ttgcgatgta gtttaagact
    43681 gcgcagtgtt aaattcttta agatacccat gggcgaatca cctcgtcact gacaatctgg
    43741 ccatcctcta aactgatgat gcgattagct tgctgggcaa tttgctgatc atgagtaatc
    43801 ataattaatg tttgctgata aagtttatta gacagcttaa gcagggcaat aatctctgcc
    43861 gagtttttac tatctaggtt ccctgttggt tcgtcagcca agagcagggc gggatggtta
    43921 atcagtgccc gtccaatcga gacccgctgc tgttgtcctc ccgacaattc attaggtaaa
    43981 tggtgcagac ggtcggttaa acctaaagtc tgcagcaatt cctgcaaata cttttgctgc
    44041 acctcctgct tgtctaagag cagcggcagg gtaatatttt cttccacatt taaaatggga
    44101 attaaattat agaactgata aatcaaaccc aagtgccgcc gccgaaagac agctaacttt
    44161 gtttcattca actgataaat atctgtgtcc ttaatcatca ctttgccgct ggttggtcga
    44221 tcaacgcccc ctagactatg cagcaaagtt gattttcccg aaccagaagc acccacaatg
    44281 gccacaaatt caccttgctt gacgctaaac gacactccac ctaaagcttt aacctgcgtg
    44341 gtaccactgc catacacttt agttaaattt tctaccctta agatctccat ctctaacttc
    44401 tccttctttt caatttacca aagttattgt aacaagcaat aataaatttc cagcaggtaa
    44461 aatcttaagt ttctcttaac tttggggtca ggcttgaata attcacttac cctaaataag
    44521 atgtaaaggc actgccagct tggcaatgcc tttttcttat tttcctgctg tgcgtttagc
    44581 ttttttgatc aacatatggc gtacaaaaaa gtagcaggcg acaccaaaaa ctgtggcgtt
    44641 aatcgccgca ccgaccataa aagttgaacc aaaaaactcc aggaagccaa aaattttttc
    44701 ttgtaaatct ccccttttta aaattatcgc atactgatgg atgataaact cacgcaggtt
    44761 ctcatggccg gctacaggtg tcagcacaat gccgccaatc aataagttta gcgcatacat
    44821 taaggggata aatggccctg tcaccagact ggtgaccgta gcactggctc ggttggcgcg
    44881 aaaaagcatg gctaataaga aagcaaaagc aaagcccaag cccagagtag ggaaaaagtt
    44941 tatccctaaa cccaagacac agcccagcgc caccttgcgc ggctcatctt tttttatctg
    45001 tttttctatt gtctcttcaa aacgtttttt aatcttttcc caagaagaaa gatttgtttc
    45061 ttctgtcaag ggagcttacc tcctctggtc tcctagcacg actaagttaa cattattatc
    45121 aaatcttgtc agccgcctaa cagattaatg ctaattttta tgttatactt ttccctcttg
    45181 acgtaaataa ataacttcaa tgtctgcaat tacaatctct cagcgaaggt ggttaaaaaa
    45241 tgtggtattt acttacactg atggtcggcg gctgtatcgg tttcttcact gccgcactgc
    45301 tcgttgctgc tcgtgacaac taacccagac acttccagtg tcttaagggc atttcaggtc
    45361 cactgtgaag cagaaaacaa tctgcaagct tgttgcagta gccctttcat ctgcacaacc
    45421 tcctcccttc tcttttatcg atattcgacg tacccagatt aatttccttt actctccctg
    45481 cgccttaaaa cttgcgtgta aagcctggaa cggtcttgac cgttcttttt ttattttacc
    45541 ttatactgtg ctgaacttac agcgtgtgac cctccggatt ttcactttat gtcgtatatt
    45601 gatgaaaaga atctaaaatt tagaatacat tctctttttc tgttgactac gttctaattt
    45661 tctgatattt ttgtattata caagaattct ttacgtagtg cactacggca aagagctagg
    45721 acaaacagtt ttttacaact aaaggaggtt ctctcatggc aaacgctcaa agaaatgacg
    45781 tgggcaaaac agaaatttgg tcttatggat ttggtgcttt gggttataat ttaggggccg
    45841 ccggtctaat gacatttctc acattcttct atactgacta tatgttaatt ccggcagcta
    45901 gcgtagcatc tattttattc ttttcccgtc tcattgatgg tggcactgat ttattactag
    45961 gagtattaat cgatcggact aaatccaaat atggtaaagc acgcccttgg ctgctttgga
    46021 tggctatccc ggccgctatt tctgtttctt tgctttacta cgtacccaat tttagcactt
    46081 ctggtagagt aatctatgct ttcatcacct ataatgctgt tgccttcttc tatctcacag
    46141 ctttagcctt acctttccaa gctttagtat cactaattac accaaacttc caaactagaa
    46201 tctctttaag ccaaatgggt gccattttca acaccttggc agccgtcttt gttaacctct
    46261 ttgcacgaaa aataatggcc gctcttggtg gcgaagcagt tggcctgtgg cgcttcttta
    46321 ccctgatggg cgtgattggc atgggcttaa tggttctctg tttctacatg actaaagagc
    46381 gcgtagtgcg taaagaaaat gaaaataaca ttcctttagg tacaggcctc aaagctctag
    46441 ccgccaataa gtactggtgg aacattacac tgctgcagtt agtgacctct ttagtaccag
    46501 cgatgtgggg tgccactgtc tactatgcac agtacatctt aaacgcacca ggcatggtag
    46561 gaccgatcat gtcgctaatg tggggcggca ttaccattgg aatcttcctc ttcatgccca
    46621 ttaccaaaaa atacggcaaa cgtaatgctg ctcaaatggg tatgattcta caggtgctgg
    46681 gttccgttgt gctctggttt aatcccactt cttcagcact gctctggatc tcaactacac
    46741 tgcgctccat tggcccggct gccttcattg ctgctggcaa cgccatgcgt gcggacaccg
    46801 tagagtatgg agaatggaaa accggtatcc gtaacgaagg gatgatctat agtggtgcca
    46861 gctttggtgc aaaagtaggc agcggtatcg gcggcgctat tctggcagct cttttagccc
    46921 gcggtggtta tgtcggtggc gcagctgtac aatctgctgc cgccatggaa gctattaaaa
    46981 cagcctttat tatcgttcct ggcattggct cctgcttaat tatggtgatg ctcgccttct
    47041 ttgatctgga gaagaaaatg ccgcaaatta tgtctgacct ggaagaacgc aaaaaaactg
    47101 ctgcttaagg caaataaaat agacaagaca gacagcgagg ctgcatatca caaacgatat
    47161 gcagccttgc tgtctgtact ccataatgtg cttttattag gtctacaccg tgtcgcaatc
    47221 taaccgacca ctgcccaggc cgcccaatca ggttgtgtaa aatagtaacg cacaatgagt
    47281 aattgtcttg ttgcccagat aatagtcgac ttttaactga tctagtgttt ccgccacctc
    47341 aataccataa tcttcgacag gcttaattct agcttcttta ctatccacaa ctcctgtcaa
    47401 gaaaatttct tgttcagtaa atttggcttt taactcctcg tacaatgaga tcactttttt
    47461 cttaaaatct cctagacttg tctcgatcgc cacagtatca accacattgc ctagcgggta
    47521 atttttagtg atcacaatca gccgttgata acgttccttt aaagctgctc ccagatcttc
    47581 ctgcgctctc aaacaatcaa tagaagcaac aacaatctca gtaaagttag tttcataatg
    47641 tcgataaaga tattttgtta gttgatcttc cgtcacaaaa acatcaaagc tagaaaaggt
    47701 gacagcattt agcacgctct tgactttttg caaggctgcc ttgatcacgc ctggttcaac
    47761 accaaaaata aatttaaacc tagccataat acttaaatca atattttctt ccgtaaattt
    47821 tattttaccg ttaataaagt ttagctgtat ttgttcaatg gagccaataa aagacatata
    47881 acgaaaggcc agcagcgggt cctcttcagc gttaatgcct tgaaaggtca tcagttcttt
    47941 aaacgtttga aagaaatttt gatacagtaa ttcagtgaca atattttctt taatggaggt
    48001 taaaaacatc tgttgtgttt tttcatcata cttcttatgc agtaaataca ttttactgac
    48061 taaaacactg cgctcaatgg gatttaaatg tggacctaat gatctaccaa gcttaataat
    48121 ggtcttgtca taatccttaa aaacatgaat taatatatcg cgtttgtttt taaaataatg
    48181 aaagataaca gagttggcga taccgacatg ctccgcaata tccttaattg tcgtggcctc
    48241 atacccctgc tcataaaata agtgcttggc agttgccaaa atatgctgtt tggtattttt
    48301 catcagcgcc actccccgtt tcatcttcag gttagtgcac aagccatgtc actaacttcg
    48361 ccatatctac gtcatttacc tgcaaaaacg ccaccagccc cttaacgggc tagtggcgct
    48421 gcatgttctg taagtatctt ttttagcttt ttggttagaa catttccttg ctcattttgt
    48481 caatttcttc gctgacggct tcgtagacgc cagggaaagt actgccagac aaacctctga
    48541 ctaaacaggg aataaagtag tgcttaccgt tagtttcaca ggctctcctt acttctttgg
    48601 caattaattc acgtgaccaa tcctcgcggt caagcaaacc attatcaaga tctcccatga
    48661 aggaaatctg tccgccgtac ttcttgacta gttccggggt gttattagtg gtcatacaac
    48721 cctgccaaac atcaattccc atctcaatca tatgggggac aaggttcgca ctgaaagaat
    48781 cattgtggtg cacaattagc tctacgccat tctctttata aaaactatat agttttttat
    48841 aggctggcac aaaaaattca gcaaacatat ccggtgctaa aaaggagtta atttggctgc
    48901 cccaatcatc atgatggaag agacattccg gcttcacata cttaactatt tctttggcat
    48961 agcccagttt ccactccgta ataaaatcaa ttagttcatg catggcttca ggttcttcgt
    49021 agaaattaat taacgtgtca tccatgccca taaaatagtg cgtctgctca aagatgccgg
    49081 gcgcaccaaa aatggtgaca aactgttcat tacggtcaat tttagctact tgcgcttgaa
    49141 tccccttcca gtcttcagga gagctttcca gggagggagc cttcagtaca tcttgccact
    49201 tggtgacatc tttaattagt ttatgttctt gatcatgaac agggaaagaa cctgggacgt
    49261 gcgctggcca tctgtgggtt acaccccaaa agtctacatt ttctccaccc aggcttgctc
    49321 tgggattacg agctgtaatg ggatccccca tgacgatccc caaatattca tactgattaa
    49381 cgaaccgatc tggcttacca cctttaattg tttctaacat attctgtctt ttacttaaca
    49441 ctacaatacc tcctttaatt gtctactgca tgtcttaagt attcgcttct gcaaattcga
    49501 ccaaatcatg cctacctact attataattc attatctagc ttcacgctac cataattttc
    49561 ttttcataag caggccagtt ctaaattagt ttgcccttga atttccttaa taaactttct
    49621 ctaaataaag aagcaaacag tttttgacac tagctagtag cttgaccaac cagggattcg
    49681 tatgcaaaaa gtgtccaggg atgtttagca gcaggatagc tggtaaaggt caaccgtgtt
    49741 tgcagtgtag gatgggtaaa agtaaggcag taagaccata gtgccaactg ctgccccggg
    49801 cgatttacgc tctgaccata acgttgatcc ccaaacagag gatgccccgc agctgcaagt
    49861 tggacgcgaa tttgatgtgc ccggccggtc tgcagctgca cctctactaa actaaaattt
    49921 ttgctgttgt ccagcacttg ataagtcagt ttagcttcct tggctccgct gctgttcttg
    49981 tcaaccaccg tcacagtatt attttgccta tttttacgta aatagtgctg cagactagcc
    50041 tgacctagtg gcagtttgcc atgcacgatt gctaaatatg ctctggtaaa agttctatct
    50101 ctcagctgcg ccgctagacg tccggcagct ttagaggttt tcgcaaaagc gatcacacct
    50161 cctgttggtc gatctaaacg ctgcaccatg cctaaataca ccttgccggg tttttggtac
    50221 ttttctttga ggctttgttt taataaactt aataagtcat cgtcaccggt attatcccct
    50281 tgtgttaaca ggttgaccgg tttttcaacc actaataaat ggttatcttc gtaaagatgc
    50341 tctattcgcc gcaatgcctg aacctccttt ttgccgcatc aggtcgtcaa tatccctgac
    50401 tgtgtgccgc tgaactgaca aaatcactca gcaaaagaac ttaaacttat ctctgcatca
    50461 tagataagct taaagatgct ataataaaga aaacgccaac tagaatcagc gcacactatt
    50521 tccactatta tacactaaac ttgctcgtgc tgtcagctta cctctagcca tgcgcaccgc
    50581 tcacatggcg ccggcagtag atccagcaaa taaataaatg acaaatgtct tcaggggagg
    50641 aagtctaatg aaatgtaact gccatcagca tggcattgcc aactgtattg agcttgtgcc
    50701 catttttagc aacttgtcca cggaggaaaa aaaagaagtg gctgccatta ccagagataa
    50761 aactttagtc aagggcgaaa tggtctatct cgcaggtgat aaaggggaaa aactttatgt
    50821 tattcacgaa gggtttgtta aaatctcccg tttaagcccg acaggtaaag aacaagttat
    50881 tcgcgtcttg ggacccggtg aatttatggg ggagttgact cttttcagtt ccctgcccat
    50941 gacggataat gccgaagcca ttgcagacac catcatgtgc atgattgaag gcccgcaatt
    51001 aaagaagcta atgccaaaat acccagccat cgcctttaaa attatggaag agttaagcta
    51061 ccggttagag aaagcagaaa atttgattga agatattagc ttacattcag tagagcagcg
    51121 tttagcccaa agcttactta gcctttcaca agcacaacct aaaatcacgc tcaaaatgac
    51181 taaaagtgat tttgcctcac aaatcggcat gagtcaagaa accttaagca ggaagcttgc
    51241 ctcgctccag gaacaaggcc taattaagtt agttggtcag cgccaaatca tcattcttga
    51301 ccgccaaggc ctagaggcac tagtttaact ataaagatct acagcaatca gccactaggc
    51361 agataaccta gtggctgatt gcttttacct tactaactgg agctaagctg gctcttatat
    51421 tgctgtatgg cattggggtc agcttgtttg gctggttgat agtacccttt ttccatggcc
    51481 ttttgactta attggaattg gaactgctca tcggcatttc taatttgaat taaagcttgt
    51541 cttaactgag agttttcaga ctcagcaata gctgtggcat agttggtcag gctagcactc
    51601 acctgggata gtacatcact caccatgtct ttatcttgca tcgttttccc tccttattaa
    51661 tttaaaaatt gcattaactt ctgtttagtc tgctgggccc tgctcgaaga atcctgaaac
    51721 atctgtttaa tctccggatc actgcattgt tgagcataag cctgcagttt gttggcacct
    51781 gtagcatgac caccaattaa gtgccgcaaa ttttctaatt cctgttgatt aatagtcact
    51841 tgcaagtctc ctttcttgta atttctgtct ttagtttgcc aacataattt agatatattc
    51901 ccctaaaaat cgcctgctaa aaaaggtaag agcaattttt tagccaagca gatgtctgct
    51961 atttttcttg catagataag agagcagctt gggataatta aaaggattac aaaacatgaa
    52021 aggagatatc agatgtttaa tccttttgat gagaaaccta tgctgctcgg tgaggccatc
    52081 atggactgga aaactatcta tccccaggct tatgacaaaa atctagttga tccctatagt
    52141 aaagtgcgta ttatcttaat gaatggtatt gaagtggagg ctgctctctt ttcccaccag
    52201 ttccaccgca actgtaacga taatgagctg cggcgtgatc tagcattttc ccggcgcatt
    52261 gagcagcagc agcaaaaacg cattaactgg ctttcaccga ttggtgaaac accgctggag
    52321 acaacgatcg gctatgaaca tgtcgcagtt gatctcacgt cctggctggc gcaaaatgag
    52381 ccggatccct atgttaaaga taccctggat tttgcgctct tagaagattt tgaccatcta
    52441 tatcgctacg ctaacctctt ggagctggat aagaatattt cagcccagaa attagttaag
    52501 aaatatgtgg aaattacgcc aggtcggcca accattgccg aacaccgtca ccccagtgat
    52561 acagtaagag gctacataaa ttttaaaagc gccgacatcc gtaccaagtt aaacattatg
    52621 attatcacgg ctgctgaaca gcagaccatg aacttttata tgaacgtcgg tccgacctac
    52681 tataatgatc tgggccgtca actgtaccaa gaaatagcat taatcgaaga agagcatgtc
    52741 agccaatacg gagccttaat ggatcctaac tgcacttggc tggagaactt gctgatgcac
    52801 gaatacatgg agtgctatct ttactactct ttctatcaag atgaaagaga tccccatctt
    52861 aaaaatattt gggagatgca cctgcagcag gaaattgctc agctgcatcg tgccaaagat
    52921 ctgctggcca aacacgaaca taaagattgg cagcaagtaa tccccggcgg cgttttccct
    52981 aaactgctgc agttccacga cactaaagac tatgtacgaa aaatccttgg tcaacagatt
    53041 gaacttactg ctgaccaaga agttcttaaa aatgttaatg acctgccgga taatcatcgc
    53101 ttcttctggt atcaacaaac agttaatcat gacgttaagg ctgtgcctag ccatgcagta
    53161 atcgctcaac atcagcagca gaaaggccag gattaccgct atgaggatca gcccaatgca
    53221 gtagaggccc tacgggatcg cagacaagac aattttaaaa ttgcccgcac taaacaaaaa
    53281 caaaggcagc tcgtcggcgt ttagaaatag caaagagtgt tgcaaactac tggcaaaagt
    53341 agctcgcaac actcttcttt taatggtttt actgattaaa ttaacatcag catatac
//
LOCUS       DASRMS010000064        23949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_887479, whole genome shotgun sequence.
ACCESSION   DASRMS010000064 DASRMS010000000
VERSION     DASRMS010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 23949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23949
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_887479"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            391..1287
                     /locus_tag="VFC74_02940"
     CDS             391..1287
                     /locus_tag="VFC74_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017211063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HZK24326.1"
                     /translation="MTKNFSKVKQVLWAILFANLAVAVMKIAVGTIIRSTSMTADGFH
                     SFTDGTSNIVGLIGVHFASQPEDQDHPYGHSKFEMFAGLFIAGMLFLIGGKIIIAAIG
                     RFFHPVLPEITSASLLVLLLTLGINIFVSIFEYRKGKALNSQILISDSLHTRSDIYVS
                     AGVLITLIGIKLGLPPLIDSLTSLVVAGFIIYAAFEILCDNGGILLDKAAVDAEKVQN
                     IVLSFDEVKDTHNIRSRGSMNDMHIDLHIMTEPDLSVEAGHDLIHSIEAKIRTEINQN
                     IQVSVHLEPYKEDAQETKGVYC"
     gene            complement(1392..1634)
                     /locus_tag="VFC74_02945"
     CDS             complement(1392..1634)
                     /locus_tag="VFC74_02945"
                     /inference="COORDINATES: protein motif:HMM:NF019367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH865 family protein"
                     /protein_id="HZK24327.1"
                     /translation="MSVRCELERQICDAIKDAPFPLMSPDALVSAFPLGIEHICRCGE
                     AEVKVSEVKEKMKDEDFPLTTAEAAAQAMLAYYPYI"
     gene            1846..2421
                     /locus_tag="VFC74_02950"
     CDS             1846..2421
                     /locus_tag="VFC74_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK24328.1"
                     /translation="MDDKRLAILTACQRLLEESGTITIDKVAAAAGVAKGTVYLYFDS
                     KQELIQQTFLASIDEISTIMAEAAAAVTGTSFQRLSAMVTAHYETVRGKMMLLQRLFW
                     DEPEMSRSPVKGQALELLHAVKQIEKQYIDVLHAGIAEGEFRTHNRQIVAAAIMAIIH
                     NLSAGEIFWPQGNQQQIMPEMLQFILQGISQ"
     gene            2858..3940
                     /locus_tag="VFC74_02955"
     CDS             2858..3940
                     /locus_tag="VFC74_02955"
                     /inference="COORDINATES: protein motif:HMM:NF013397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015499 - formate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015724 - formate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HZK24329.1"
                     /translation="MSKRCQFWQDYDSVSGGISYCELAAFNQPVNPQFLAQIGCTEQK
                     RTECLGTMQLNIGGAAVPVVQPSAFNAPGEIAKSCVGAAEKKAAIAPRPLSVLAFLAG
                     AYIAMAGILNTVITNDLANFFGDGLTRLVGGTVFSLGLILVILGGAELFTGNNLVMTA
                     GLLDKKITISQVMRNWVTVYFFNFVGSITVALLVYYSGIWKFNGTAVGIKAVNVAAGK
                     VALPFSEALVRGILCNWLVCLAVWLSMSAKDAIGKLLCCALPVVAFVAMGFEHSVANM
                     YFIPMGILLSGKEAIVAAIGAEALQSLTWSAFLVKNLLPVTIGNMIGGIVFVAATYYN
                     AYLRPATVKQEPAATVKAAPAHHQHS"
     gene            3971..4426
                     /locus_tag="VFC74_02960"
     CDS             3971..4426
                     /locus_tag="VFC74_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK24330.1"
                     /translation="MDNTVKCPHWREYQGLNGWVMFCSAGAFAQTFNKVEAEHIGCTE
                     ENRVTCKRIMELNLGYALQPEIIEVAEQAQEAIPAAVPLHFPDVAAQKKNLNDYPDLL
                     TVAMVSDYLELSHSKTLSLIKNGSIPVVHSDGEVKVEKAKLEEWLKCTA"
     gene            4695..5843
                     /locus_tag="VFC74_02965"
     CDS             4695..5843
                     /locus_tag="VFC74_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HZK24331.1"
                     /translation="MYISKFVAPEIIFGSGSINQVGECCYRLGASRIFLVADRGVIKF
                     GWVEKCLPYINELNLDYRIWSDFSANPKDFEIEAGAALYLQQQCDAVLAIGGGSAIDA
                     AKAVAILATNGGRIQDYEGIDLIQKPLPPLIVLPSTAGSGAEVSQFSIIADSERNIKM
                     TIVSKSLVPDIAISDPLILSTVNSRVTATTGMEALSHAIEAYLSLAATDLTDVHALHS
                     IKLIANHLRASVASQVNEDAKVAMAKASLQAGIALSNAVLGLTHAMTHQVGGLLDLPI
                     GEISAVLLPHVMRFNMISSFEKFGAIAEAMGANTSKLSARDAAKQAIEKVQELAGDVG
                     IPSGLSALGLPKEVIPELSKNAINDACYITNPRDVDVDGIVSIFYQAL"
     gene            5865..7565
                     /locus_tag="VFC74_02970"
     CDS             5865..7565
                     /locus_tag="VFC74_02970"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK24332.1"
                     /translation="MVITNKTTLIEKLTGIHSSKKSYYVELKKKIDELSKKNIQLEII
                     NQLAKSIGVNMSLNEIIENFIPKMRTLVYFDLLSLYIVSNDGKIKRVTFYQDEQQQNK
                     IEESILETEQLQQDLEAENSQTTQFLRRVFQQQEPILANNVAVQALPEDAALVKQQTM
                     SKVIIPLLVKEKIIGILELVSQQEFDDDCLSFISQVADQLAVCLENMELYNEVWQHKH
                     EWEITFSAVTDLLVFINEDLEIQKVNQAAKDFFTLKEEEIIGQKCYKMFYGRNSKCNP
                     CLGEQVLQTKKTAYTQSRTRFNRILDIYVYPSYTEEEQPYGITYYAKDVTRIVDSIKF
                     VSLGEMSAGVAHELNSPLTAIVGNSQLLLRETAEDNPHYQLVKDIQYCGVRCQGIIRN
                     LLTFSRQDELSFEWLYLDQVFEKAYSLLAYQVEKSGIEVRKNISAPLPAILGNAQGLE
                     QIIVNLILNAKDAIEGKYGRQNEAVQSNGVIEIRTRVQPDKFVALDIVDNGCGIDPAL
                     IPHIFDPFYTSKSIGKGTGLGLSVSLGIAQSHKGLIDVQSKPGGGSTFSLILPIGKTE
                     "
     gene            7611..9212
                     /locus_tag="VFC74_02975"
     CDS             7611..9212
                     /locus_tag="VFC74_02975"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZK24333.1"
                     /translation="MAEKVKFLVVDDDQEVCNFFTYLLKSKGYEVETAQTGRETFAKL
                     DSTRFNAALVDLKLPDSDGITILKKIKEQQPECEVIIITGYSTVKSAIEAIQYGAFDY
                     VEKPFVDIEEMEKLLERALSVSTQSAEAIIEINGLTFIVGESPKMRRLLTAARKIATK
                     NITVLIQGETGCGKEVLARYIHAISHRNDRAFLACNCGAFSESLLESELFGHEKGSFT
                     GAISRKRGIFELAHKGTLFLDEIDSASLAIQVKLLRVLETGEFLRVGGEDICKVDMRI
                     IAATNANLATRVEESHFREDLFYRLDVASFTIPPLRERTEDLPLFINYFLEREHKLKQ
                     TPVKRFSKEALAVMQEHPWPGNIRELKNTVSQALVFSSGPLITVADLPERIRQKKHHL
                     PEATVTPKLESEPPTKPTPELPSPPPASPEKPALTPAGPQEQAPTVPAEGALAAASVP
                     VVLQPQQEHLLEHLAEFEAKLVETLRLKAGFAFNDFYDSFKQWNDQLNKKIIAKALEE
                     TYDNQVKAAELLGITPRALRYYLNK"
     gene            9470..10195
                     /locus_tag="VFC74_02980"
     CDS             9470..10195
                     /locus_tag="VFC74_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24334.1"
                     /translation="MIELAGVSKSYNKGAIKAVDNLNLSVRPGEIFGFLGPNGAGKTT
                     TIKMMVGLLQADSGTITINGHNIEQEPLQVKSSLSYVPDNPEVYEKLSGLEYLNFLAD
                     VYRVPAKQRKERMERFLQLFEMTQAVGDLIQSYSHGMRQKIVLIGALLHEPEVFILDE
                     PMVGLDPKSAHNLKEYMREHCDQGKTVFFSTHVLEVAEKLCDRIGIIRRGQLIACGTM
                     DELKQQAQNEGSLEKIFLELTEA"
     gene            10192..11859
                     /locus_tag="VFC74_02985"
     CDS             10192..11859
                     /locus_tag="VFC74_02985"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24335.1"
                     /translation="MRLLTLIKMQLNVSFNWSALKWYAKHDKKKILGGIALLLFILVS
                     LAPVYYFLAINFLETVYLSGYSLGQPEFILTFAIVAVSMLVLFFAIPFIMATFYFSRD
                     LALLVPLPFLPHEIIGAKFSTVVIQEYLTVLPFMLPALYFYGTGQNAGPYFWLVGILL
                     TLLLPIIPLAIISAAILFLMRVTNLGKRKDLLRYLGMMLMLAAVLGFNYFMTKLEPMT
                     QEELMNLLFAKEGLATLISRVYPPALWATRALAAEGTTALLNLAMFVILTSMGVMLTL
                     YLGDRLFYRGLIGGDEVTARRDISVDRLAKAMSQVSSPAWAIAKREIKVLLRTPIYFF
                     NSVAVILIMPLAMFLPLIWGNAMEPILTMLETLAPLYLNLGAAASIALVAAFAPAAST
                     SFSREGKQFWLSQVMPVVPQEQINGKIIYSLLLSLLGIPLVFLASFLLFHWTNQELIL
                     ISLVGMFLTLPSITVSLLIDLLRPYLTWSNPQAAIKQNFNAVFGMVATGVLYYLMYQF
                     AMYMLQQQYSDFVIYSGVVASSLLLAALPYGIMVKIAERRFREIIAP"
     gene            12096..12296
                     /locus_tag="VFC74_02990"
     CDS             12096..12296
                     /locus_tag="VFC74_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24336.1"
                     /translation="MNLSTKEVNYLKDFLSWELAGAKRCAAYAKDTVDPNLAKLHDAS
                     SRTHQDNFVDLLNYVNAKAKEV"
     gene            12300..12668
                     /locus_tag="VFC74_02995"
     CDS             12300..12668
                     /locus_tag="VFC74_02995"
                     /inference="COORDINATES: protein motif:HMM:NF019490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HZK24337.1"
                     /translation="MDPNATITTETGTQKIGKQVTINFTKDQTINDLDRLYDMIAHCK
                     HMLSVYQTASSEVIDPELYSLLNTCKQRVQEQHGKAVDTLFNMGEYTADVAPPEQVKD
                     ISAVFMGYLKQLPNQNKLPH"
     gene            12834..13313
                     /locus_tag="VFC74_03000"
     CDS             12834..13313
                     /locus_tag="VFC74_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008962 - phosphatidylglycerophosphatase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HZK24338.1"
                     /translation="MKLLQPNEMVDSIYDINLHQLKANGIKGIITDLDNTLLAWRSSE
                     FKPTVEQWISEVRNANLKIAIVSNNSTARVEDLSAQLGVVCVAKAIKPRRGAFRSIAA
                     QFNLAPHELAVVGDQLFTDVLGGNRAGMYTILVDPISRHEFIGTKLMRLLEKLFLRR"
     gene            13380..13523
                     /locus_tag="VFC74_03005"
     CDS             13380..13523
                     /locus_tag="VFC74_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24339.1"
                     /translation="MEKHLQRLKEAYQQAKQEQQTANFTLQNITINTQTKEFTFHWQD
                     ENK"
     gene            13744..14571
                     /gene="aroE"
                     /locus_tag="VFC74_03010"
     CDS             13744..14571
                     /gene="aroE"
                     /locus_tag="VFC74_03010"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZK24340.1"
                     /translation="MQITSQTKVTGLLGDPVIYSLSPAMHNAAFQYLQLPYVYLPFQV
                     DKSRLAGAVEAIRALNICGVNVTVPHKETVIPYLDHLAESAVRCGAVNTIVNGAGVLT
                     GYNTDGQGFIDALLETGFNPSAKKAVILGAGGAARAVAASLLDNGTREIVLINRTLAK
                     AEKLAHFLGSQASCQQLIPDSSPDLANVDLVVNTLSIPFRQDDKWLLNLTAAQGALFY
                     DLRYGKMPSDFLVLAEELNSPRLDGLGMLLHQGARAFTLFTGTEAPLEIMRYALIGV"
     gene            14864..16075
                     /locus_tag="VFC74_03015"
     CDS             14864..16075
                     /locus_tag="VFC74_03015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24341.1"
                     /translation="MTKLKPDSHPRRLKKEQAWPKLSIGRRIFKKALVLLLFFAMFFS
                     VVWVPLTDAFFTAEDSSVKTTFIVPKVDRSAKADAKMNFEAPMVIENMAEKSVSPKLT
                     KEAAAAVDLQTGQEEAAEPIVWARLQLQGRFVAGDIFLPSLKLHYQGHTTKAQQGELI
                     AANTLRVAFTRTELVAWFKALDQAAAQTLTITGEGYRGGLDHFSFTGTGILEFEEQVL
                     PPEPVNEEEAPVETPQDEERAEAAPVVTAMQISGPVNIFIPVSPTSTSAAYTVEVKDQ
                     NNQLLKNEEVTWSLAEEAAGVVISANGDLAVNTTADAGTVTVVAVAQSNKEVSATLLV
                     TLAQEAVTPPADTEEKTASAEQDTPQTEAPPAEEPKQADPPPPVEATHTEPEVVSEEK
                     DDPASGETSVE"
     gene            16105..16752
                     /locus_tag="VFC74_03020"
     CDS             16105..16752
                     /locus_tag="VFC74_03020"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HZK24342.1"
                     /translation="MEEETALAEATKQQSGQLWRKALQISGNLVFALLLLMLVLLTIT
                     TVQSRLKGKPPQLAGYQMYIVLGGSMSPTFEMGSLAFLKPIAAQDVVAGDIITYSSPF
                     DAASLTTHRVMEIHDDNGQLYFTTRGDANDVNDLVPALAANVVGKVVFALPYLGYVLH
                     FGRSKLGLIMLVVLPGFIIMLIELRNILRYYTEWKQSKESQAANEETAVEADSPS"
     gene            16825..17826
                     /locus_tag="VFC74_03025"
     CDS             16825..17826
                     /locus_tag="VFC74_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24343.1"
                     /translation="MKARVIRSILIFLAAAILFTGGVTSAWFIDTATPEPATMVAGTV
                     KLNVLPAQVTPVPQDPQQITWQPGTNKTFCWSLKNTGSKKIMLRARPLLERETKGETA
                     WAEGAPFNPDGQGNWAMYFTYNVGSGKKSVRLLAGQHEYCGDLIVQTINNSLHVTYQL
                     KQGYALLASHLAVTANFADIPTVANGNPPPGQFPFKKEHNAVQEYTYIIPLSGTYPAG
                     HLKGQSYSWGNGQELYLAAHSDVLVPNSGSAKNISWQLATQSSAGWQKGEPETVDGKT
                     VTWWYYCQPVPANNEIKLCLTGYLAEKAASGTYQIHLEAEAVQASHDAIDIWPHPCK"
     regulatory      17920..17997
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            18037..18735
                     /locus_tag="VFC74_03030"
     CDS             18037..18735
                     /locus_tag="VFC74_03030"
                     /inference="COORDINATES: protein motif:HMM:NF023806.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HZK24344.1"
                     /translation="MKKRVLMSVLVLALAAALIAGGTMAWFTDTAEANPAVFKAGTVE
                     IEACGLTHLTPCFKADNWNPGDCSWVGVDFKNVGTKKIKLRVKMDKAWEGQLSTGNVE
                     ISVPHALRLWWEYKDGYFYYKFPFIGKAVSPGVYTVPLLFEVCLSGPNTGNQYQGKTF
                     TINAIGEAVQFSHNAPWSAVATNGSVQGPQSAGLTVETAPDITDMTDDAAIAEMQDLQ
                     AEYNKLVAEIEAAQ"
     gene            18809..19438
                     /locus_tag="VFC74_03035"
     CDS             18809..19438
                     /locus_tag="VFC74_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24345.1"
                     /translation="MKRKIISSVAMILGILLIISSLAMMAKPGVVFALELVGDELGLR
                     LEPSNTALFDVKNMNPGDTKQAGLSICNGGKCPFTAYLSMVKVSGTRGKGDLYPQLEM
                     TVTYLDEEIYHGPVSGFQQYKLGQLQPNDNQPLDFTVHLPGPTTGNEYQGSTLGVKLV
                     FTATQECTPPTPPKPDIPATSGFDTIVLLAGMSSLLFGILLRRRKMVSK"
     gene            19775..21508
                     /locus_tag="VFC74_03040"
     CDS             19775..21508
                     /locus_tag="VFC74_03040"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZK24346.1"
                     /translation="MVLSTKIRRGLGAISLSSLLMLLVFIWRRHFDLYFAIVAVLLHV
                     GCFLAALALIDKVLLQRVQALIKQLNPVLQTETLDAKQDELSDVALALQQLYEKNNEY
                     LQQVLILENLADLVSYLDSEMNLYYASQSHRTLLGYEPKFLLNSARNTDNHNDFFHPD
                     DIERVFASAVNVTKTLEPERIELRIRHADGHYLWFESVHTPFFSTAGELKGVLVSGRD
                     ITERKVMEDQLRQMSWHDSLTGLFSRSYFELQMQHFEFNRQAAFGIIAVDINDLKLYN
                     DGLGHAAGDILIKQTASLLKKSFRENDVVARIGGDEFAILLPQSSIETVQNAVQRIKE
                     AVVTYNQSNPKLPISISLGYAIGEPDTSVRDVLKEADNNMYSEKMRRNISDSSSTIAI
                     LMNTLESRDYRPDNDAILLRDLVLEFAEHLDYPPEKYANLSLFARFHDIGKVGIPDRI
                     LYKGGRLTETEKKEMEQHCEIGHRIALSALDLVPVAEWIFKHHEWWNGQGYPQQLQGE
                     EIPLECRILAIAEAYHAMLSFRPYRQAFSHVEAIAELRRHAGSQFDPELVEEFIALLA
                     VEKQKTLTQMD"
     gene            complement(21505..22923)
                     /locus_tag="VFC74_03045"
     CDS             complement(21505..22923)
                     /locus_tag="VFC74_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HZK24347.1"
                     /translation="MKLVKLLSKLVARLRPQRDSEATEEEKLYCSQVAAIPYFLAPLV
                     FLALQSLTATFGALDLVVPDLPIPINLETGRAFHLNLSILWPLLGCMGASYYFLVEET
                     KRELHSLRLVTLQFWIFVLTTIGILTSLMFGYLRGMEYEEAAFPFHVSIAVTLAIFFY
                     NVLRSYLKAPKYRGRMTLLAIVSGSVFLLLLYVPNIINYNQPTVSELVRYWVIHLWEE
                     LSKELILFGVLAAFLLKVSSLERKRLERILFVQITLLIIGATFATGHHYYWIGLPTLW
                     QWVGGIFSGVQIISISLLLYILYLGLTHLNWIDLDLGLKLTFGFIIASVCYHIMGAAS
                     LGMVIALPQINRYSSATYLTSAHAHFALYGALGMMVLAISTYVLTRKAELTRKEYLLS
                     WWGFGLNNLGLLTMGAALTIAGVLQTYLQRVAGIDFMATIAFLRSYLFIRTLGGACFA
                     AGALLYAKQILWCYWRNRHYFN"
     gene            complement(22920..23342)
                     /locus_tag="VFC74_03050"
     CDS             complement(22920..23342)
                     /locus_tag="VFC74_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HZK24348.1"
                     /translation="MKRTEVGFLVITLATLGGFALLIYYSLVQVTPPMPPAAVAGSEL
                     WQEYGCMECHAILSSGGYSAFDLTKIASQLSEKELREFFAQPPHMPPHSKRIHVSLTE
                     READEMIAYLRYVSEIDTRNWPPPPVVEEMGLKERKNK"
     gene            23544..>23949
                     /locus_tag="VFC74_03055"
     CDS             23544..>23949
                     /locus_tag="VFC74_03055"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK24349.1"
                     /translation="MEVKELKLTLWNISREITENINNIFYPLAEKHGLTLRQLQVLLN
                     LLQHEEHTVGSLGKCMSVAGGNISNLCKKLEREGFVERCRKTDDERVVVVTLSPKGRQ
                     TISEIDQIVDRKYELLLRNHSQEFLSEIVVGLS"
BASE COUNT         6784 a         4929 c         5569 g         6667 t
ORIGIN      
        1 aataataccc gtgcagcatt gtgctacaag ggtttctggc ttttcgtttc aggttaagct
       61 gtcaaactaa cgggtaactg ctttgtcaca gaacaaccag tccctagaca aatgcgacgc
      121 ttgataggaa aactgcttgc cgcagtttgt attgacatat tgaggttgtt tcgcatatag
      181 tatagttaaa gaaaattaat ttgatcatga gtcaggcgag ggagccgggc cgcgtttggc
      241 agcagatata aatctgtggg actatttata gtttcacaga tttttttatc gggtcagaag
      301 ttttaataac cttgaattgc tttattgcca atccgcacgg gcattacaaa aaagcgtaag
      361 ctttaatctg ctgaaagaga ggtgattttt atgactaaaa actttagcaa agtgaagcag
      421 gtactgtggg ctattctctt tgccaatcta gctgttgcgg tcatgaaaat agcagttggt
      481 actattatta gaagtactag tatgactgct gatggctttc actcctttac agatggcaca
      541 tcaaatattg tggggttaat tggtgtgcat tttgcttccc aaccggagga tcaagaccat
      601 ccctatggtc atagcaaatt cgaaatgttt gccgggttgt ttattgccgg gatgttattt
      661 ttgataggtg gcaagatcat cattgctgct atcggcaggt tttttcatcc tgtactgcct
      721 gagataactt cagcaagtct gctcgtcttg ctgctaacgc tgggcatcaa tatttttgtg
      781 agtatttttg aatatcgaaa aggtaaagca ctaaacagcc agattctgat ttcagattct
      841 ttgcatacca gaagtgatat ttatgtctca gccggggtgc taataaccct gatcggtatt
      901 aaattgggtt taccaccgct cattgactct cttacctcac tggttgtggc gggcttcatt
      961 atttatgcag cgtttgagat tttatgtgat aatggtggta tcctgcttga taaggcagcc
     1021 gttgatgcag agaaggtgca gaatattgtc ctgagctttg acgaagttaa agatactcac
     1081 aatattcgca gcagaggcag tatgaatgat atgcatattg atttgcatat tatgacggag
     1141 cctgacttaa gtgtagaagc aggtcatgat ttaattcata gtatcgaagc aaagatcaga
     1201 actgaaatta accaaaatat acaagtgagc gtccatttag aaccttacaa ggaagatgcc
     1261 caggagacaa agggagttta ttgttaagca gaagcactcg ctcactaact gaagaatttt
     1321 gccgggaaaa cacaagatga cagggggacc ctgtcatctt gtttatgggg tgctattacg
     1381 aagttgcgct cttagatgta tggataatag gctaacatag cttgagcagc tgcttcagct
     1441 gtggtcagag gaaagtcctc atctttcatt ttttccttga cctcgctgac tttcacttca
     1501 gcttctccgc aacggcagat atgttcgata cccaggggga aggctgaaac caaagcgtcc
     1561 ggcgacatta gggggaatgg cgcgtccttt attgcatcac agatttgtct ttccaattcg
     1621 catcttactg acattatgca caactccttt cgaactttac ttgttatcat tataaaagca
     1681 aagttctatg aagttcttaa taaaaaatta taaacggaaa aagttgtaag cacatcttgc
     1741 agagacactt tggcagctta ttgacatcga ggctaagcat gatataattt ttttgcgact
     1801 gactatgaag tcagccgtat tatgctgcta ctagggggaa taaaagtgga tgataagcgt
     1861 ctggctattt taactgcttg tcaacgttta ttagaagaaa gtggtacgat tactatcgac
     1921 aaagtggcgg cggccgccgg ggtagcgaaa ggaaccgtct atctttactt tgatagcaaa
     1981 caagagctga ttcagcaaac ttttttagcc agcattgatg agatttcaac aattatggca
     2041 gaggctgccg ctgctgtcac agggacaagt tttcagcgtc tatctgcaat ggtaacggcg
     2101 cactacgaga cggtgcgtgg taagatgatg cttttgcagc gcttgttttg ggatgagccg
     2161 gaaatgtcac gcagtcctgt caaagggcag gctttagagc tgctgcatgc cgtcaagcag
     2221 atagagaaac aatacatcga tgtattgcac gccggtattg ctgaaggcga gttccgaacc
     2281 cacaataggc aaatagttgc cgctgccatc atggcgatca ttcataacct tagtgcaggt
     2341 gaaatttttt ggccgcaagg taatcaacag cagatcatgc cggagatgct acagtttatt
     2401 ctacaaggga ttagccaata acttggggtc aggtttaacc taagaaccta tctttttatg
     2461 aagccgctta gcggcttttt tattacaatt ctttttgcag gtagggatgg gagcggcacg
     2521 gtcaagaccg ttccctactg ttgtagaggt ggtgacgaat tttaaaggta gcgatgtagg
     2581 tttctgcgag cgtgatgtaa gtttatcggg gtgcgaggca ggttttgtga gtagtgacgt
     2641 aaataggtaa cttgtcttgt gacgtaagtg cgtaaacttg tattgaatgc aacaataaaa
     2701 aggtattaaa gtgacgtaga taggtaacac tgacagtaac taggcaaaaa atagggcgac
     2761 ccctgcccat tatgctcttg tgaaagttgg cacaactctt gcaatgataa agggtgaaga
     2821 caaaaaaaga gataagtaat aaaaggggtg aaagaaaatg tcaaaaagat gtcagttttg
     2881 gcaggattac gattcagttt ccggcggtat ctcatattgt gaacttgcag cttttaacca
     2941 gccggtcaat ccacagtttt tagcacaaat tggttgtacg gagcagaaaa gaacagaatg
     3001 tttaggtacc atgcagctta atattggtgg tgccgctgta ccggtagttc aaccttcagc
     3061 attcaatgca cctggcgaga tcgccaaaag ttgcgttggt gcagcagaga aaaaagcagc
     3121 tattgcacca cgcccgctct cagtgcttgc ctttcttgcg ggtgcctata tcgccatggc
     3181 cggcatttta aacactgtta ttaccaacga tttagcaaat ttttttggtg atgggctaac
     3241 tcgtctcgtc ggcggcacag tattctcact aggactcatt ctggttattc tcggtggcgc
     3301 agaactattt acaggtaata atttagttat gactgcgggt ttgcttgata aaaaaatcac
     3361 tatcagccag gttatgcgta actgggtcac agtttacttt tttaacttcg tcgggtccat
     3421 tactgttgcc ctgttagtct attactccgg tatttggaaa tttaatggta cagcagttgg
     3481 cattaaagct gtcaatgttg ccgctggtaa ggtggccctt cccttctccg aagctttagt
     3541 aagaggcatc ctttgtaact ggttagtatg tttagctgtt tggttatcaa tgtcggctaa
     3601 agatgctatt ggcaaactgt tatgctgcgc acttcctgta gttgcttttg tagccatggg
     3661 tttcgagcac tcagtggcca atatgtattt tatccccatg ggaatcttat tgtctggtaa
     3721 ggaagcaatc gttgccgcca tcggtgcgga agctttacaa agtttaacct ggtcagcatt
     3781 ccttgtgaaa aacctgcttc ctgtaacaat tggtaatatg attggtggaa ttgtcttcgt
     3841 agcagcaact tattacaacg cttatttacg tcctgcgact gttaagcaag aacctgccgc
     3901 aacggtcaaa gcagccccag cccaccatca gcattcttaa gaaccttaaa gtaagaaagg
     3961 agtagtctca atggataata cggttaaatg tccacattgg agagaatatc aggggttaaa
     4021 tggttgggtg atgttctgca gcgccggtgc ttttgcgcaa acttttaata aagtggaagc
     4081 tgagcacatt ggttgtacag aagaaaatag agtgacatgt aaacgcatca tggagctcaa
     4141 cctagggtat gctctacagc ctgaaatcat cgaagtagca gaacaagcac aagaagctat
     4201 ccccgctgcc gtaccgcttc attttcctga tgtcgcagca cagaaaaaga acttgaatga
     4261 ctaccccgac ttgttgactg tggccatggt ttctgattac cttgagctca gtcatagcaa
     4321 aacactttca ctaatcaaaa acggttctat tccggtggtg catagcgacg gcgaagtaaa
     4381 agtggaaaaa gcaaaactcg aagaatggtt aaaatgtact gcctaaaaaa tgtttgtcgc
     4441 catggtgcct aagaaatagc attcttaggc actttttctt acttttcctg ccggtagagt
     4501 tgtctggtaa cttatagttg catagtctag aagagcacat tagaatcttg tttacagtca
     4561 agaccgcggg aatagtttta acggcactaa aaagggacaa acttttctca ttatacaaat
     4621 aattagacat cctttagcag gataatgtat cctttgtgag gaaaataaca atgttaggga
     4681 ggaggtaatg acagatgtat atcagtaaat ttgtcgcacc cgaaattatc tttggcagtg
     4741 gctcaatcaa tcaagtaggc gaatgttgtt atcggctggg agcttcacgt attttcttag
     4801 tagctgaccg cggcgtgatt aaatttggtt gggtcgaaaa gtgcctccca tacatcaatg
     4861 aactaaattt agattacagg atttggtcag attttagtgc gaatccgaaa gattttgaaa
     4921 tagaagcagg agcagctctt tatttgcaac aacagtgtga tgccgttttg gcaattggcg
     4981 gcggcagcgc gattgatgct gctaaagcgg tagctatctt ggctactaac ggtggtagaa
     5041 tacaagacta cgagggtatc gatttaattc aaaaaccctt gccaccactg attgttcttc
     5101 catccacagc cggttcgggg gctgaagtgt cacagttttc tattattgct gacagtgaac
     5161 gtaatataaa aatgacgatt gtttctaaat cactggttcc cgatattgcg atatccgatc
     5221 ccctgattct ttctacagtt aatagtcggg ttaccgccac cacaggcatg gaagcactca
     5281 gccacgccat cgaagcgtat ttatcactgg cggctactga tttgacagat gtacatgcgc
     5341 ttcattcaat taagttaatt gctaatcatt tgcgggcgtc agtcgccagt caagttaatg
     5401 aagatgccaa agtagccatg gctaaagcaa gtctacaggc aggtattgcc ctatctaatg
     5461 ccgtcttggg cttgacacat gccatgacac accaggtagg tggcttgttg gatttgccca
     5521 tcggggaaat cagtgcagtt cttttacccc atgtgatgcg ttttaatatg atttcctcct
     5581 ttgaaaagtt tggtgctatt gcagaggcta tgggtgcgaa caccagtaaa ttgtcagcgc
     5641 gggatgctgc caagcaagcc atcgaaaaag tacaggaact ggcaggtgat gtggggattc
     5701 ccagtgggct ttcagccctg ggtttgccca aggaagttat tcccgaactt agtaaaaatg
     5761 ccattaatga tgcgtgttat attaccaacc cgcgagatgt ggacgtagac gggattgtga
     5821 gtatttttta tcaggcgtta taatttgctt aaggagatta gaagatggtt attacaaata
     5881 aaaccacgct gatcgaaaag ctaacaggaa ttcatagctc caaaaaatct tactatgtgg
     5941 aactaaagaa aaaaatcgac gagctttcaa agaaaaatat tcaactggaa attattaatc
     6001 agttggctaa aagtattggc gtcaatatgt ctcttaatga gattattgaa aactttattc
     6061 ccaagatgcg aacgcttgtt tattttgatc tacttagtct gtatattgtg agcaatgacg
     6121 gcaaaataaa acgagtaact ttttatcaag atgagcaaca acaaaataaa attgaagaaa
     6181 gcattttgga gaccgaacag cttcagcagg acctggaggc tgagaacagt caaacgacac
     6241 aattcttacg gcgagtcttc cagcagcaag agccaatctt ggccaacaat gtagcggtgc
     6301 aagccttacc tgaggatgca gcactagtta agcagcagac aatgtctaaa gtcattatcc
     6361 ccttgctggt taaagaaaaa attattggca tcctggagtt agtcagccaa caagaatttg
     6421 atgatgattg tctttccttt atctcccaag tggccgacca gctcgccgtc tgcctggaga
     6481 atatggagct gtataatgag gtttggcagc ataagcacga atgggaaata acattttccg
     6541 cagtaacgga tttacttgtt tttatcaacg aggatttgga aatacagaaa gttaaccagg
     6601 cggctaaaga ctttttcact ttaaaggaag aagaaattat tggtcagaag tgttataaaa
     6661 tgttttacgg gcgtaatagc aagtgtaatc cttgtttagg cgaacaggtt ctgcaaacta
     6721 aaaaaactgc ttacacccaa tcgcgaactc gcttcaaccg tattttagat atctatgtct
     6781 atccttctta tacagaggag gaacaaccct acggcattac ttattatgct aaagatgtga
     6841 cgcgcattgt cgactccatt aaatttgttt ctttggggga aatgtctgcc ggtgttgcgc
     6901 atgaactaaa tagtccctta acagctattg tgggtaattc acaattattg ttgcgtgaaa
     6961 ctgctgaaga taatccccac tatcagctgg ttaaagatat ccaatattgt ggcgtacgct
     7021 gccagggcat tattcgcaat ttgctcactt tttcccgtca ggatgaactg tcatttgaat
     7081 ggttgtacct ggatcaagtc tttgagaagg cttactcctt attagcatat caggttgaga
     7141 aaagcggtat cgaggttcgc aaaaatattt ctgcaccact gccggcaatc ctcggtaatg
     7201 ctcaaggttt agagcaaata attgtcaatt taattctcaa cgctaaagat gccattgaag
     7261 gcaaatatgg tcggcaaaac gaagcagtac aaagtaatgg cgtgattgaa ataagaacga
     7321 gggtgcagcc tgataagttt gtggctttag atatcgtgga taatggctgc ggcattgatc
     7381 cagccctgat cccccatatc tttgatccct tttacaccag taaaagtata ggtaagggca
     7441 cggggttagg tctttccgtc agcttgggca tcgcccaatc tcataaaggc ttaatcgatg
     7501 tccaaagtaa accgggagga ggcagtacct ttagtttaat cctaccaatt ggtaagacag
     7561 agtaacagtt cccttcactg ttagcatctg cagagagagg aggtgaagcg atggctgaaa
     7621 aagtaaagtt tttagtagtt gatgatgatc aagaagtttg caattttttt acctatttgt
     7681 taaaaagcaa aggctatgaa gtagagacgg ctcagacagg tcgggaaaca tttgccaagc
     7741 ttgactctac tcgcttcaat gctgccctgg ttgatttaaa gttgccagat agtgatggta
     7801 ttaccatcct caaaaagatt aaagaacagc agcctgaatg tgaagtgatc attatcaccg
     7861 gttattccac agtcaaatca gcaattgaag ctattcaata cggagcgttt gattatgtgg
     7921 aaaaaccctt tgttgatatt gaggaaatgg aaaaactgct ggagcgggcg cttagtgtca
     7981 gcacacaatc agcagaggcc attattgaaa ttaatggtct aacctttatc gttggtgagt
     8041 cgccgaaaat gcggcgcctc ttgacggcag cccggaaaat agccacaaaa aatattacgg
     8101 tacttatcca aggagaaacg ggttgcggca aggaggtttt agcacgttat atccatgcca
     8161 ttagccatcg caatgaccgc gcctttctcg cctgtaactg tggtgctttt agtgaaagcc
     8221 ttttggaaag tgaactattt ggtcatgaaa agggatcctt caccggtgcc atcagtcgta
     8281 aacgcggcat cttcgaactt gctcataagg gtacgctttt tcttgatgaa attgactcgg
     8341 cttcactggc gattcaggtt aagctgctgc gtgttctgga aacaggcgaa tttttacgcg
     8401 tcggtggtga agatatttgc aaggtggata tgcggattat tgcggccact aacgccaacc
     8461 tggcaacgcg agtagaagag agtcatttcc gtgaagactt attttatcgt cttgatgtag
     8521 cttcctttac catccccccc ttgcgggagc gcactgaaga tctgcctctt tttatcaatt
     8581 attttttaga aagggaacat aaacttaaac aaactccggt gaaaagattt agcaaagagg
     8641 ctttagctgt catgcaagag catccttggc caggtaacat tcgcgaacta aaaaatactg
     8701 tttcgcaagc tttagttttt agttccggtc ccttaattac cgtggcagat ttgccggaga
     8761 gaatcagaca gaaaaaacat catctgcctg aagccacagt aacgcctaag cttgaatcgg
     8821 aaccaccaac aaagccaaca ccagaactgc cgtcacctcc accggcatca ccagaaaagc
     8881 cggcactaac gccagctggc ccgcaagagc aagctcctac agtcccggca gaaggcgctc
     8941 tagctgcagc ttcagttccc gtggttctgc aaccccagca agagcactta ctagagcatt
     9001 tagctgagtt tgaggctaaa ctggttgaaa cgttgcggct caaagcaggc tttgctttta
     9061 atgattttta cgatagcttt aaacaatgga atgaccagct aaataaaaaa atcatcgcta
     9121 aagccttgga ggaaacgtat gataatcaag tcaaagctgc tgaactatta ggtataacac
     9181 cacgagcgct gcgctattat ttaaataaat aaactttgta aaataacaag cagctccgct
     9241 aaagtgggag ctgctttatg gtagatctat cgtggtgtag ccggctttgt ttacttcgta
     9301 ggggcggcac tttgtgcgcc cgtacctcct aaaatataaa tcatccatag tcataacttt
     9361 gctgtaatct gcattgcgat aatttaacag atccttaaaa ggaataatat tgctgtgcga
     9421 gaataaataa agaggacatt aatactaaat ctataaggag gttggccaga tgattgagct
     9481 agcaggtgtc tccaaaagtt ataataaagg cgccattaaa gcagttgata atctaaacct
     9541 gtctgtcagg cctggagaga tttttggctt tttagggcca aatggagcgg gtaaaacgac
     9601 taccattaaa atgatggtag gtctcttaca agctgacagt ggtacgatta ccattaatgg
     9661 tcataacatt gagcaggagc cgttgcaggt caaaagctca ctttcctatg tgcccgataa
     9721 cccggaggta tatgaaaaac tttcaggctt ggaatactta aactttctgg cagatgtcta
     9781 tcgcgtaccg gccaagcagc gcaaagaaag aatggagcgt ttcctgcagc tttttgaaat
     9841 gacgcaagcg gtaggggatc tcatccaaag ttattctcat gggatgcgcc agaagattgt
     9901 cttgattggt gctctactac atgaacctga agtattcatt ctcgatgagc cgatggttgg
     9961 tctggatcct aaatcagcgc acaatttaaa agaatatatg cgtgagcatt gtgaccaggg
    10021 caaaacagtc tttttttcta cccatgtttt ggaggtggca gaaaagctgt gtgaccggat
    10081 tggcatcatt cgccgcggtc aattgattgc ttgcggtacg atggatgaac taaagcagca
    10141 ggcgcaaaac gaaggttcgc tggaaaaaat cttcctggag ctgacggaag catgagactc
    10201 ttaaccttaa ttaaaatgca gttaaatgtc agtttcaact ggtccgccct gaaatggtat
    10261 gctaagcacg ataagaaaaa gattctgggc gggatcgcgc tacttctatt tattcttgtc
    10321 agtttagcac ccgtttatta ttttctcgct attaatttcc ttgaaacagt ttatttaagc
    10381 ggttactccc tcggccaacc cgaatttata ctcactttcg ccatcgtggc cgtcagtatg
    10441 ttggtactgt tttttgctat tccctttatc atggccacat tctatttttc ccgtgattta
    10501 gcattgctgg tgcctttacc ctttttgccc catgagatta ttggagctaa atttagcacc
    10561 gtggtcatac aggaatatct tacggtttta ccatttatgc tgccggctct ctatttttat
    10621 ggtacagggc aaaatgccgg cccctatttt tggctggttg gtatactgct tactttactg
    10681 ctgcccatca tcccccttgc cattatttcc gctgccattc tttttctgat gcgggttact
    10741 aatctgggca aaaggaaaga cttactgcgt tacctgggca tgatgctgat gctggcagct
    10801 gtcttgggtt ttaactattt tatgaccaaa ctggagccga tgacgcagga agaattaatg
    10861 aatttgcttt ttgccaagga gggtttggcc actctgatct cccgtgtcta tccgccggcc
    10921 ttgtgggcga cccgtgcttt agcggcagag gggactactg cactgttgaa tctggccatg
    10981 tttgttatcc tgaccagcat gggagttatg cttacattgt atctcggcga ccgtcttttc
    11041 tatcgagggc tgattggcgg tgatgaagta acggcacgca gggatatttc tgtcgaccgc
    11101 ctggcaaaag cgatgtcgca ggtttcatct ccggcctggg cgattgccaa acgcgagatt
    11161 aaagtgctgc tgcgtacacc tatttacttt tttaatagtg tcgccgtgat cttaattatg
    11221 cctctggcca tgttcttacc actcatctgg gggaacgcca tggagccgat cctcaccatg
    11281 ttagagacac tagcgcctct ttatctgaac ttaggggctg ccgcctccat tgccctggtg
    11341 gctgcttttg ccccggctgc ctcaacttct ttttcccgtg agggtaagca gttttggcta
    11401 tctcaggtca tgcctgtggt cccacaagaa cagattaacg gtaaaattat ctattcacta
    11461 cttttgtcct tgttgggtat tcccttagtg tttttagcgt catttttgct cttccattgg
    11521 acgaaccaag aattaattct gatcagcctg gttggcatgt tccttacttt accgtccatt
    11581 accgtcagtt tactaattga tctgcttcgc ccctatctga cctggtctaa tccccaggcg
    11641 gccataaagc aaaatttcaa cgcagtcttt ggcatggtgg ccacgggcgt tctttactat
    11701 ctcatgtatc agtttgccat gtacatgctg cagcaacagt atagtgattt cgttatctac
    11761 agcggtgttg ttgcctcttc actgctgttg gcggccctac cttatgggat tatggtcaag
    11821 attgcagaga ggcgtttccg tgaaattatt gccccctaaa ccacaaaata cgactatagt
    11881 acagatagcg ccggctggtt aagccggcgt ttttatcttc ctgtaaatag aatcgtgcga
    11941 ttacaagcag tttttttgtt aatcctgcca ggttatgcaa gtactagtta taaacattta
    12001 taatattgct cagtctgtga agtggctaaa ctgaatgtta tgcatagcaa atattattta
    12061 acaaacacta gcaagagaag aagggaggcc taaacttgaa tctatcaact aaagaagtaa
    12121 actacttaaa ggactttctg tcgtgggaat tagcaggggc aaagcgttgt gctgcctatg
    12181 ccaaggacac tgtagacccc aatcttgcca agctgcatga tgccagcagc cgcacacatc
    12241 aggacaactt tgtagatctg cttaattatg tgaatgctaa agccaaggag gtgtagtaag
    12301 tggatcctaa tgctactatt accacggaaa caggtaccca aaaaattggc aaacaagtta
    12361 ctatcaattt tacgaaagac caaactatca atgatttaga tcgtctttac gatatgattg
    12421 ctcactgtaa gcatatgtta agtgtttacc aaactgcttc ttcagaagtt attgaccccg
    12481 agctctacag cctgcttaat acctgcaaac aaagggttca ggaacagcat ggcaaagctg
    12541 ttgatactct ctttaatatg ggtgagtata cagcagatgt ggctcccccg gaacaggtta
    12601 aagacatctc agctgttttt atgggttatc ttaagcagct ccctaatcaa aataagctgc
    12661 cacactaaag catagttaaa taattgttaa tggagacttc caccgaagtc tccctcgctt
    12721 tacagggctt aatctactgc taattcccag ggcggttagc ttttattttt tttcttctcc
    12781 aattcctgct ttctatgcta taataaaggg tatctatatt tttgaggtga gtaatgaaac
    12841 tattgcaacc aaatgagatg gtcgattcca tctacgatat caatctacat cagctaaaag
    12901 caaatggcat caaaggaatc attaccgatt tggataacac ccttttagcc tggcgttctt
    12961 ctgaatttaa accaactgtg gaacaatgga ttagtgaagt acgaaatgct aacctgaaaa
    13021 tcgccattgt gtctaacaat tccacggcac gggtagaaga tctttcggct caactgggtg
    13081 tagtttgtgt tgccaaagcg attaaacccc ggcgtggtgc tttccgcagc attgctgccc
    13141 aatttaattt agcgccgcac gaactagcgg tggtgggtga tcaattgttt accgatgtgt
    13201 tgggcggcaa tcgtgccggt atgtatacaa tcctggttga tcccatcagt aggcacgaat
    13261 ttattggcac caaactaatg cgcctgctgg aaaaattatt tctccgacgg taggaaacaa
    13321 gctttttcca tgtataaact tttacctctg ggtaacacta ccggcagagg tggtacgata
    13381 tggaaaagca tctgcaacgc ttaaaggaag cttatcaaca agctaaacag gagcagcaaa
    13441 cagctaattt cacactacag aatataacaa ttaacacgca aacaaaagag tttactttcc
    13501 actggcagga cgaaaataaa taagccgcac ggcttatttt tatttatggc aaaattatca
    13561 taaaaagtag gggcgaactg tgttcaccag tacgtcctgc tgcatgatta tcgcacttca
    13621 agtgtagtga atggtcctga gatgctgaaa tagctaacaa ctgctgccaa tgtgttaacg
    13681 gttctctgtg taagagagga ttttcttgct atgctgcaga atagttttgt cgaggtgaga
    13741 actatgcaaa taacaagtca aacaaaagtt acagggcttt taggtgaccc tgtaatatat
    13801 tccttatcac cagccatgca taatgcagct tttcaatatt tacaactgcc ttatgtttat
    13861 ctaccctttc aagttgataa aagccggctg gcaggtgccg ttgaagcaat ccgggctctt
    13921 aacatctgtg gtgtgaatgt cactgtgccg cataaagaaa cagtgatccc ctaccttgac
    13981 cacctggctg aatctgctgt tcgctgtggt gcagttaata ccattgttaa tggggctgga
    14041 gtcctgactg gctacaatac tgatgggcaa ggctttattg acgctcttct ggaaaccggt
    14101 tttaatccca gcgctaaaaa agctgtaatt ctcggtgctg gcggcgccgc acgtgctgta
    14161 gccgcatctt tgctagataa cggtaccaga gaaattgtcc tcattaatcg caccttggct
    14221 aaagcagaaa aattggccca ctttttagga tcacaagcat cttgtcaaca attaatacct
    14281 gatagctccc cggatcttgc caacgtcgat ttggttgtca atactctttc tattcccttt
    14341 cgccaagatg ataagtggct gctgaaccta acagctgccc aaggagcgct gttttatgat
    14401 ctccgttacg gcaagatgcc gtcagatttc cttgttcttg ctgaagaact aaacagtccc
    14461 aggcttgacg gtctgggcat gctgctgcac caaggtgccc gcgcctttac actttttaca
    14521 ggcacagaag caccactgga gattatgcgc tacgccttaa taggagtgta gtaacgctgc
    14581 agcttactaa ctcatgcgcc tgcattcttt gtgtattaac ttaagtgcaa aaataaacgt
    14641 ggaacggtta taaccgttcc tttttttatg cttttttttg taccttgctc tgccaaagga
    14701 aagggttaag gaacggtcaa gaccgttctt ttttgtcggt gtagctaaca ttccaatagt
    14761 aaaaaagaga gcaataataa acaaaaaaat caggttataa atatcataaa aaactatata
    14821 catggattag aaaatgattg agctgttgga agggggagcc atggtgacaa aactaaagcc
    14881 cgattcgcac ccgcgccggc ttaagaaaga acaagcatgg ccaaagctga gtatcggcag
    14941 gaggatattt aagaaggcac tggttcttct tctgtttttt gcaatgtttt tttcggtcgt
    15001 gtgggttccc ttgacagatg cgttttttac agcagaagat agttcagtaa aaactacctt
    15061 catcgtacct aaagtagata gatcagcgaa agctgatgct aaaatgaact ttgaagctcc
    15121 tatggtcatc gaaaatatgg cagaaaaatc agtatctccg aagttaacta aagaagccgc
    15181 agcagctgtt gatttacaaa ctggccaaga ggaggcggca gaaccaattg tctgggcgcg
    15241 cctgcagctg cagggccgtt ttgtggcagg cgatattttt ctgccctcgc tgaagcttca
    15301 ttaccagggt cacacgacaa aagcccagca aggtgaattg atagctgcca ataccttacg
    15361 tgttgctttt acgcggactg aactagtggc ctggtttaag gcgctggatc aagctgcggc
    15421 gcaaaccctg actattactg gtgaagggta cagaggcggg ttggatcatt tctcgtttac
    15481 tggcacaggc atattggagt ttgaagaaca ggtgctgcca ccagagccgg ttaacgagga
    15541 ggaagcccca gtcgaaacac cgcaagatga agagcgagca gaagctgcac ccgtggttac
    15601 tgccatgcaa atcagtggcc ctgttaacat ttttattcca gtatcaccta cttccactag
    15661 tgctgcttat acagtcgaag tgaaagatca aaataatcag ctgctgaaaa atgaagaagt
    15721 tacatggtcc ttagcagaag aagcagctgg tgtagtcatc agtgccaatg gtgaccttgc
    15781 tgtcaatact actgctgacg ctggcaccgt aactgtggta gcagtagcac aaagcaataa
    15841 agaggtgtca gcaacacttc tggtcacact ggcacaagaa gcggttacac cgccggcaga
    15901 tacagaggag aagacagcat ctgctgaaca agatactcct caaacagagg cgccaccggc
    15961 tgaggagcca aagcaagcag atcctcctcc gcctgtagag gcaacgcata cagagccgga
    16021 agtagtaagc gaggaaaaag atgatccggc ctcaggagaa acgtcggttg aatagtacct
    16081 gttgtgtcga gaggaggtaa agtgatggaa gaagagacag ctttagctga agctacgaag
    16141 cagcaatcgg gccagctgtg gcgcaaagct ttacaaatca gcggtaattt agtttttgct
    16201 ttgcttttgc taatgttggt tctacttact attaccacag tgcaaagtcg tctcaaaggc
    16261 aagccgcccc agcttgctgg ctaccaaatg tatattgtct taggtggcag catgagccct
    16321 acctttgaga tgggaagttt agcttttctt aaaccaatcg ccgcacagga cgtggtggca
    16381 ggcgacatta ttacttacag ttcacctttc gatgccgcaa gtcttaccac gcaccgggtg
    16441 atggaaattc acgatgataa tggtcagctt tattttacaa cccggggcga tgccaatgac
    16501 gtcaatgatc tagtcccagc attagccgct aacgtagtag gtaaggttgt ttttgctctg
    16561 ccatatttag gctacgtttt acattttggt cgctctaaat tgggactgat catgttagtg
    16621 gtgctgcctg gttttatcat catgttaata gagctgcgca atatattgcg atattacacc
    16681 gaatggaaac aaagcaaaga gtcgcaagca gcaaacgaag agactgccgt cgaagcggat
    16741 tccccctctt aaccagctta tttgcattta gcttctcgct agcgttaatg taggcagaat
    16801 taaggtaaag aaaggaagtt tcagatgaaa gccagagtaa tacggagcat cctcattttc
    16861 ttagccgcag ccatactatt taccggcggt gtgacttcag cctggtttat agatacggct
    16921 acccctgagc cggccaccat ggtagcaggg acggttaagt taaatgttct gcctgcacaa
    16981 gtcacacccg taccacagga tccgcagcag attacttggc agccaggtac aaataaaact
    17041 ttctgctggt cactaaaaaa cacaggcagt aaaaagatca tgttacgagc ccgaccgctg
    17101 cttgagcgtg aaactaaagg agaaacagct tgggcagaag gtgccccctt taaccctgat
    17161 ggccaaggta actgggcaat gtatttcacc tataatgtgg ggtcgggcaa aaaaagtgta
    17221 cgccttttgg ctggccagca tgagtattgt ggtgacttaa tagtgcagac aataaataat
    17281 tccttgcatg ttacttatca gctgaagcag ggctatgcat tattagcttc tcatcttgct
    17341 gttaccgcta attttgctga catacctact gtggcgaatg gtaatccacc gccaggtcag
    17401 tttcccttta aaaaagaaca caatgctgtt caggaatata cctatatcat acccttaagc
    17461 ggcacctacc ctgctggaca tttaaaaggc caatcctata gttggggcaa tgggcaagag
    17521 ctatatctag ccgcccactc tgacgttctg gttcccaaca gtggcagtgc taaaaatatc
    17581 tcctggcagc ttgctacaca aagctctgcc ggttggcaaa aaggtgaacc ggaaacggta
    17641 gatggcaaaa ctgtgacctg gtggtattac tgccagccag tacccgcaaa taatgaaatt
    17701 aagctctgcc taacaggtta tttagctgaa aaagcagcca gtggcaccta tcagatccat
    17761 cttgaagccg aggctgtgca ggcatcccat gatgctatag atatctggcc gcatccctgt
    17821 aagtaatacc agtttgcatc aaggtgattg tcaacactgg ctaccaaata aaggggtggt
    17881 aaactggata agcataagta aaaagtcgtt ggtgtcgtaa tccgctcagg gcaaacctat
    17941 tgtgaggtag ggacgcaaaa ctacgggcct ttgattagag gcagccgggt taccggagaa
    18001 gtagggtaaa gtaagctaga aaatggaggg aaaagaatga aaaaaagagt tttaatgagc
    18061 gtgctagtac tggcactggc ggctgctctg atcgcgggtg gcacaatggc gtggtttact
    18121 gatactgccg aagctaatcc agcagtgttt aaagctggaa ccgtggaaat tgaagcctgt
    18181 ggcttaacac acctaacacc atgttttaaa gcagacaact ggaatccggg ggattgcagt
    18241 tgggtgggcg ttgacttcaa gaatgttggc actaaaaaaa ttaaattaag agttaagatg
    18301 gataaagcct gggaaggcca attgagtacc ggtaatgtgg aaatatctgt tcctcatgcg
    18361 cttcggctct ggtgggaata caaagatggc tatttctact ataaattccc tttcatcggc
    18421 aaagcagttt cccctggtgt ctatacagtg ccgcttctct ttgaggtatg cctaagtggt
    18481 ccgaacacgg gtaatcagta tcaggggaaa acatttacca tcaatgcaat tggtgaagca
    18541 gttcagtttt ctcataatgc tccttggtct gcagttgcaa ctaatggttc tgttcaagga
    18601 cctcaaagtg ctggtttaac tgttgaaact gcaccagata ttacagatat gactgacgat
    18661 gcagcaattg ctgaaatgca agatttacaa gctgaatata acaagctcgt agcagaaata
    18721 gaagctgcac aataacataa ttcagttagt agagtaagat agtttttgct ataagcagac
    18781 gatagtcgtg tgagagggag gactcgtgat gaagaggaaa ataatcagct cagtggcaat
    18841 gatattgggg atcttgctga tcataagcag cctggctatg atggccaaac cgggtgttgt
    18901 ctttgcactc gagctggttg gtgatgagtt gggcttacgc cttgagccct ccaacacggc
    18961 tctctttgat gtaaaaaaca tgaatcccgg tgatactaaa caggcaggtc tgagtatttg
    19021 taatgggggt aagtgccctt ttacggccta cctgagtatg gttaaagtca gcggtaccag
    19081 aggtaaaggc gatctctacc cgcagctgga aatgacggtt acatacctgg atgaagaaat
    19141 ttatcacgga ccggtaagtg gttttcagca gtataagctg gggcagcttc aacctaatga
    19201 taaccaacct ctagacttta ctgttcatct gccgggtccg acaacaggta atgagtatca
    19261 gggtagtacg ctcggggtaa aactggtgtt taccgctaca caagagtgta cgccccccac
    19321 tcctcccaaa cccgatattc cggctaccag cggtttcgat acaatcgtgc tgctagcagg
    19381 catgagcagc ctcttgtttg gtatcttgct gcgcagaaga aagatggtca gcaaataaat
    19441 agttgcaaaa aagagtgtgt cttcggcaag gagatgcact cttttttgca tgatttgtaa
    19501 ttaacaaaac cgtgtatctt ttcgtatagt tgcacctctc gaccatccca atttgtgtat
    19561 agtaggttat tgttgaaaag aaagtttggc ggcgcaaaag tgagtttagt cattaatttg
    19621 caagatattt gtcgatattt aaaattcgag caggaatatt gtgtcattgc aggaatttat
    19681 atagatgtat tcctagtttt tcgtagtaac tatttagacc acagacagta gcttaatttc
    19741 ctccttaaat atcattagat agggtgtgtt gctgatggtt ctatctacta aaatacggcg
    19801 tggcctggga gcaattagtt taagctctct actgatgctg cttgttttta tctggagacg
    19861 tcatttcgat ctttactttg ctatagtggc agtactttta catgtgggtt gttttttagc
    19921 tgcgctagcc ctgatcgaca aggttttact tcaacgtgta caagccctaa tcaagcagct
    19981 aaacccggtt ttgcaaacag aaacactaga cgcgaagcag gacgagctta gtgatgtggc
    20041 attggcgctg cagcagttgt atgaaaagaa taatgaatat ctacagcagg ttttgatctt
    20101 ggaaaacctt gcggatttag tctcctatct tgactctgag atgaatcttt actatgccag
    20161 tcagagtcac agaactttac ttggctatga acccaaattt cttctaaata gtgcccgtaa
    20221 tactgataac cataacgatt tttttcatcc agatgatatc gagcgtgttt ttgcttcagc
    20281 agtgaatgtg actaagactt tagagcctga acgaattgaa ttgcgaatta gacatgctga
    20341 cgggcattat ctctggtttg aatctgtgca tacgcccttt tttagtacag cgggggagct
    20401 aaaaggggtt ttagtaagtg ggcgggatat tactgaacgc aaggtcatgg aggatcagct
    20461 gcgtcagatg agttggcacg actcgctgac cggtcttttt agtcgttctt atttcgagct
    20521 gcaaatgcag cattttgaat ttaaccgtca agcggctttt ggtattattg ccgtagatat
    20581 caatgattta aaattgtata atgatggtct cggccatgcg gcaggagata ttctcattaa
    20641 acaaactgca agtctcctga agaaaagttt ccgtgaaaat gatgtggtgg cccggattgg
    20701 cggtgatgaa tttgccattc ttttgcccca aagcagtata gagactgtgc agaatgcagt
    20761 gcagcggatc aaagaggctg ttgtcacata caatcagagc aatcctaaat tgcccatcag
    20821 tatttcactt gggtatgcta ttggtgaacc agatacatca gtgcgtgatg tattaaagga
    20881 agcagacaac aatatgtata gtgaaaaaat gcgacgcaat attagtgatt ccagctccac
    20941 cattgcgatt ctcatgaata ctttagagtc tcgcgattat cggccagata atgatgctat
    21001 cttattacgg gatttagtgc ttgaatttgc tgaacacctt gattatccgc cggaaaagta
    21061 tgctaacctc agcttatttg ctcgttttca tgatattggt aaagtaggta ttccagaccg
    21121 gattttatac aagggaggca gattaactga gacagagaaa aaggaaatgg agcagcactg
    21181 tgaaattggt caccgcattg ccttatcggc gttggattta gtgcctgtgg cggaatggat
    21241 ttttaaacat catgaatggt ggaacggcca gggctacccc caacaattgc agggtgaaga
    21301 gatccctctg gagtgtcgga tactggcgat tgcggaagcc tatcatgcga tgttaagctt
    21361 tcggccttat cgacaagctt ttagtcatgt agaagctatt gccgagctgc gtcgccacgc
    21421 tggcagccaa tttgacccgg agctggtgga agaatttatt gctctgctgg cagtagagaa
    21481 gcaaaagaca ctaacacaaa tggactaatt aaagtaatga cgattgcgcc aataacacca
    21541 aagaatttgc ttggcataaa gcagtgcgcc tgcagcaaaa caggcaccgc cgagagtgcg
    21601 tatgaacaag taggaccgta gaaaagcaat agtagccata aaatctattc ctgctacccg
    21661 ctgcaaataa gtttgtaaaa caccggcgat agttaaggct gcccccatgg ttagcaagcc
    21721 cagattattg aggccaaacc cccaccagct tagcagatac tcttttctgg ttaattcagc
    21781 tttacgcgtt aagacgtagg tagagatagc tagtaccatc atccccaggg ctccataaag
    21841 ggcaaagtgg gcatgggcgg aggttaagta agtagcacta ctataacggt tgatttgtgg
    21901 cagggcaata accatgccca gtgaggctgc ccccataatg tgatagcaga cactggcgat
    21961 aatgaagcca aaggtaagct taaggcccag gtctaaatct atccaattaa gatgtgtcag
    22021 gcccagatag aggatgtaaa gtagcaaaga aatgcttatt atctgcactc cgctaaaaat
    22081 accaccaacc cactgccata aagtaggtag gccaatccag taatagtgat gaccggtagc
    22141 aaaggtagcc ccaatgatca gcagcgttat ttgcacaaag agaattcttt ccagtctttt
    22201 tcgctctagg ctgcttacct ttaaaagaaa tgcagctagt accccaaaaa gaattaattc
    22261 tttgcttaat tcttcccaca ggtgaataac ccagtagcgg acaagttcgc tcacagtagg
    22321 ttgattgtaa ttaatgatat tgggcacata aagcagcaaa agaaagacag agccgctgac
    22381 aatggcaagt aaggtcatgc gcccccgata cttgggtgct ttaaggtaag agcgcaggac
    22441 attatagaaa aaaatagcca gagtaacggc aatactgaca tgaaagggga aggctgcttc
    22501 ttcatattcc atgccccgta agtagccaaa catcaaggaa gttaaaatgc cgatagttgt
    22561 gagcacaaaa atccagaact gcagtgtgac caggcgtaga ctgtgcagtt ctctttttgt
    22621 ttcctccaca agaaagtaat aggaggcgcc catgcagccc aagagcggcc ataaaatact
    22681 caaatttaaa tgaaaagccc gccctgtttc cagatttatc ggtatcggta aatctggcac
    22741 aaccagatca agggcgccaa aggtggctgt aaggctttgt agagctagaa agacgagcgg
    22801 tgccaaaaaa tatggtatcg ccgccacctg tgagcagtaa agcttttctt cctctgttgc
    22861 ttcactatca cgttgaggcc tgagtctcgc cactagtttg cttaatagct taacaagttt
    22921 cacttgtttt tcctttcttt aaggcccatt tcttctacca ctggtggtgg aggccaatta
    22981 cgtgtatcaa tttcactaac atagcgtaaa taagcgatca tttcatctgc ctctcgttca
    23041 gttaagctca catgaattct tttgctatgg ggaggcatgt gtggcggctg ggcaaaaaac
    23101 tcacgcagtt ctttttccga tagttgggag gcaattttgg tcagatcaaa ggcggagtaa
    23161 ccaccactgc tcaagatcgc atgacactcc atgcagccgt attcctgcca gagttcgctc
    23221 cctgccactg cagccggtgg cattggtggt gtaacctgca ccagggaata gtagataagc
    23281 agggcaaaac cacccaaagt agccagtgta attactaaaa aaccaacttc tgtacgtttc
    23341 atgagtctcc tcaatcgcta aagcatttta cttgtattct tttccttgct tgcgattata
    23401 tacaagaaag actactaatg ctgttatacc gctgccagtt atggttctgg cattacctaa
    23461 tcacctattg acaaaaacta atgtggaata taatatatat aatatataag tgttttaata
    23521 aatagccttg cctggaggaa acgatggaag ttaaagaatt aaaacttact ctttggaata
    23581 ttagcagaga gattacagaa aatatcaaca atatctttta tccccttgct gaaaagcatg
    23641 gtttaacgct aaggcagctg caggtgttac ttaacttatt gcagcatgaa gaacatacgg
    23701 taggcagtct gggtaagtgt atgagtgtag ccggcggcaa tatctctaac ctgtgcaaaa
    23761 aattggagcg ggaaggtttt gtcgaacgct gcagaaaaac tgatgatgaa agagtggtag
    23821 tagtgacact ctcccctaaa gggcgccaga cgatcagtga aattgatcag attgtagacc
    23881 gtaagtatga gctgctatta agaaaccatt cgcaagagtt tctgagtgag attgttgtgg
    23941 gattaagtc
//
LOCUS       DASRMS010000065         1215 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_552735, whole genome shotgun sequence.
ACCESSION   DASRMS010000065 DASRMS010000000
VERSION     DASRMS010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552735"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VFC74_03060"
     CDS             complement(<1..259)
                     /locus_tag="VFC74_03060"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017407605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK24350.1"
                     /translation="MKIAVAIPCYKVRKYVLEVIEQIPDSVQRIYAVDDKCPEASGQF
                     VEENCTDPRVTVLYNSSNRGVGGAVLSAYRQALVDNMDIVVK"
     gene            complement(277..624)
                     /locus_tag="VFC74_03065"
     CDS             complement(277..624)
                     /locus_tag="VFC74_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HZK24351.1"
                     /translation="MSISIIALTLLCVLGIASGQILFKKAAMHLPSNPQLMDWLLNGW
                     LITALALYGVATLLWIWVLRHAPLHIAYPFMALAFIIVPLLSYYFLGEPLTTKTFIGG
                     ALIILGVSLSVVH"
BASE COUNT          357 a          265 c          267 g          326 t
ORIGIN      
        1 ttttcaccac aatatccata ttatcaacaa gggcttgccg gtaagcgctc agcactgcgc
       61 caccaacacc gcggttactg ctgttataca gcacagtgac ccgggggtct gtacaattct
      121 cctctacaaa ctggccgctg gcttctgggc atttgtcatc cactgcatat atacgctgta
      181 cagaatcagg gatttgctca ataacttcga gcacatattt acgaacttta tagcaaggga
      241 tagcaacagc aatcttcata gagttttcca atcttcctaa tggactacgc tcaggctgac
      301 accaagaata atcagggcac cgccgataaa ggttttagtg gtgagcggct cgcctaaaaa
      361 gtaataactg agtaatggca caataataaa agccagtgcc ataaacgggt acgcaatatg
      421 caaaggggcg tgtcgcaata cccaaatcca cagcagcgtt gcaacgccgt ataatgcaag
      481 cgccgtaatc aaccagccgt tcaacaacca atccataagc tgcgggttgc tgggcaggtg
      541 catggcagct tttttaaaaa gaatttgtcc agatgcgatg ccaagtacac ataggagtgt
      601 gagtgcgata atagatatag acataaaggc tctgcggtaa ttaaatgttt agaatttata
      661 cctagtggga tgtgttttag tatagcttta gacatttaaa tgtatgataa atttaaaacc
      721 gcctcattct acctgaattt agcaaaaaaa gactgataag aggggtgagc tagctcaatc
      781 atgttggctg aacgtttaat gcttctcagg ttcccgtata gtcacggcag tttcaactga
      841 agtgcttgca gctagcacag tttttttgcg agctaattag atctgcgtgg cagaggcaga
      901 gccgtttgat aggtttcttg tattatcgtg ctaacgtcgt tagacgtgaa cagcttgaag
      961 ttacattttt agagttaggg aacctctgaa aatctcagct tattattgag cttttcagaa
     1021 aatgactcga aaactcaaaa aaacagctaa aaaaggccgt tttcgaggta aaaccacctc
     1081 ttttagccct taattcgccg ttttggcgga ttaagggcgc actaacccta agccagcaag
     1141 tatttcctac cgatcatgag gttcgttagc cccgccagca ggcacaaacg gttatggttt
     1201 ttatcaagcc ctttg
//
LOCUS       DASRMS010000066         1472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_82743, whole genome shotgun sequence.
ACCESSION   DASRMS010000066 DASRMS010000000
VERSION     DASRMS010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1472
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82743"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1472
                     /locus_tag="VFC74_03070"
     CDS             <1..>1472
                     /locus_tag="VFC74_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24352.1"
                     /translation="VALSGILNAVAPIVTQIINWLSKIITYISAFIAMLSGKNTITVA
                     KKQNESYAKSLDKTAKAANKAQLALAGFDELNNLTTQKDSGGSGGTSPSSMFEEKEIG
                     SILPELIRNWFGKLKTAIQSGDWYSVGAIIAEGLNAAVSKAHDTIKKINFKQIGASIA
                     TSINGAIHTIDWHKLGNTLSDGIGGLLDAIIEMLSTLDWESLGSSIAEFITAIDWEGI
                     TQRIAEGAGLLIGGVLKSIWGIVKTAIANEALGWAGEDGKLTAEEFIKGFVETWINIK
                     LWNFKNIAVPLGKKLAESLFGINEEMIKAGIDAINGFFSGILSKSDFGKWIKDNIYTP
                     FITWFKKLFGIASPSIVMEEQGRYVAEGLLNGITDKLKNIAAWVQNNIFMPVMNAVKK
                     AFGVAGNVASRFLEIGSALVAGVKNGISNAWGAFSSWVERQFSSIITGAKKLFGIHSP
                     SKVFARIGEFIMRGLYVGIDGEKDTALKSVADIASTMEDT"
BASE COUNT          450 a          282 c          387 g          353 t
ORIGIN      
        1 gcgttgcctt gtcgggcata ctaaatgcgg tcgcgccgat agtcacgcaa atcatcaact
       61 ggttaagcaa aattataacc tatatttcgg cctttatcgc tatgctatca gggaagaaca
      121 caataacggt agctaaaaag cagaatgaaa gttatgctaa atcgcttgac aagacagcta
      181 aggctgcgaa caaggcacag ctcgcccttg caggatttga cgagttaaat aaccttacta
      241 cccagaagga cagcggcggc agcggcggca cgtccccaag cagcatgttt gaagaaaagg
      301 agatcggctc tattcttccg gagctgataa gaaactggtt tggtaagcta aaaaccgcaa
      361 tacaatcggg cgattggtac agcgtaggcg ctattattgc cgaagggctt aatgctgccg
      421 tgtcaaaggc gcacgataca atcaaaaaaa tcaattttaa gcagattggc gccagcatag
      481 cgacatcaat aaacggggca atccatacca tagattggca caaattgggg aacacgctgt
      541 cggatggtat aggcgggcta cttgatgcga ttatagaaat gctatcaacg ctggactggg
      601 agagtttagg gagtagcatt gccgaattta taaccgccat agactgggaa ggtattacac
      661 agaggattgc cgaaggggca ggattgctta taggcggggt actgaaaagt atatggggta
      721 ttgttaaaac ggcaattgca aatgaagctt taggctgggc tggcgaagat ggcaaactta
      781 ccgccgaaga atttattaaa ggattcgttg aaacgtggat aaatataaag ctatggaact
      841 ttaagaacat tgctgtaccg cttggaaaga aattagctga atcacttttt ggcattaacg
      901 aggaaatgat taaggcggga atcgatgcta taaacggctt ctttagcggg atactatcaa
      961 aatcagattt tggcaaatgg ataaaggaca atatctatac gccgtttata acatggttta
     1021 agaagctttt cggcattgcg tccccgtcca tagtgatgga agaacagggg cggtatgttg
     1081 cagaaggcct gcttaacgga atcactgata aacttaagaa catcgcggca tgggtgcaaa
     1141 acaacatttt catgcctgtt atgaatgctg taaagaaagc gtttggcgtt gccggaaatg
     1201 ttgcaagcag gtttttagaa ataggctcag ctcttgttgc aggtgtgaag aatggcataa
     1261 gcaatgcgtg gggcgccttt tcgtcatggg tcgagcgtca attctcaagc atcatcacgg
     1321 gagcaaaaaa actctttggg attcactccc catcaaaagt atttgcgcga atcggcgagt
     1381 tcattatgcg cggcttgtat gtaggcatag acggcgagaa agatacagcg cttaaatcgg
     1441 ttgcggatat cgcgtcaacg atggaggaca ca
//
LOCUS       DASRMS010000067         1368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_687330, whole genome shotgun sequence.
ACCESSION   DASRMS010000067 DASRMS010000000
VERSION     DASRMS010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1368
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_687330"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1274)
                     /locus_tag="VFC74_03075"
     CDS             complement(24..1274)
                     /locus_tag="VFC74_03075"
                     /inference="COORDINATES: protein motif:HMM:NF021143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HZK24353.1"
                     /translation="MKKLFTFMVAVFLTATLWAQSPEKMSYQAVIRNSSEALVTDTQI
                     GMQISILQGSASGTAVYVETQVPTTNANGLVSLEIGAGTVESGDFATIDWANGPYFIK
                     TETAVEAPLTTYTITGTSQLLSVPYALNAKTAESVTGGITETDPVYSAWDKTTGISIT
                     ESQISDLGNYIETETDPSVPIGTQIGEMQYWNGTAWVTVAATENEGAKLQMIGGVPTW
                     IGGTPPPTPNVTNPTTGKTWMDRNLGASQVATSSTDAAAYGDLYQWGRAADGHESRTS
                     GTTSILATSDTPGHGNFITNGSSPYDWRNPQNNNLWQGVSGTNNPCPSGYRLPTEAEW
                     EDERTSWSSNNAAGAFASPLKLPVAGRRYSSGGSLGGVGSSGLYWSSTVDGTGSRGLY
                     FNSSSAGVDSYRRAYGSSVRCLKD"
BASE COUNT          308 a          319 c          311 g          430 t
ORIGIN      
        1 aaattatcaa ataaatcaaa gtgtcaatcc ttaaggcaac gcacagagct tccgtacgcc
       61 cggcggtagc tgtccacgcc ggcactgctg ctgttgaagt acaagccccg cgaaccggta
      121 ccatctacgg tactcgacca atagaggccg ctggaaccca caccgccgag cgaaccaccg
      181 ctgctgtagc ggcggcctgc cacgggcaat ttaagcggag aggcaaaagc tccggctgcg
      241 ttattgctgc tccaacttgt acgctcgtct tcccattcgg cttctgttgg cagtcggtaa
      301 ccgcttgggc aaggattgtt agttccgctt acgccttgcc aaaggttatt gttttgcggg
      361 ttgcgccagt cgtatggact actgccattg gttataaaat tgccatgccc tggagtatca
      421 cttgttgcca atatagatgt tgtacctgat gtacggcttt cgtgaccatc ggctgcacgc
      481 ccccattggt acaagtcgcc ataagcggca gcatcggtac ttgaggttgc aacttgcgat
      541 gcaccaagat tgcgatccat ccatgtttta ccggtggttg ggttggtaac atttggtgta
      601 ggaggtggtg ttccgcctat ccatgtaggt acaccaccta tcatttgtag ctttgctcct
      661 tcgttttcgg ttgctgcaac agttacccat gctgtgccgt tccagtattg catttcgcca
      721 atttgggtac cgatgggaac gctggggtcg gtctcggttt caatataatt acctaagtcg
      781 ctgatctggc tctcggttat tgaaatgcct gttgttttat cccatgctga ataaacaggg
      841 tcggtttcag taattccacc tgttactgat tcagcagttt tagcatttaa agcataaggc
      901 acacttaaca actggcttgt tcctgtaatg gtataggttg ttaatggtgc ttcaactgct
      961 gtttcagttt taatgaaata aggaccgttt gcccagtcta tggtggcaaa atctccactt
     1021 tcaactgttc ctgcacctat ttctaaactt accaaaccat tggcattggt ggttggcacc
     1081 tgtgtttcaa cataaactgc cgttccgctt gcagaacctt gtaaaatgct gatttgcatt
     1141 ccaatttgtg tgtcggttac taaggcttcg ctactgttgc ggattactgc ctgataactc
     1201 attttttcgg gactttgtgc ccaaagtgtt gccgttaaaa atacggctac cataaatgta
     1261 aataactttt tcattgttgt cctccttaat tttttatgat tttaaaagtt ttgaattctt
     1321 tgttttttgt atcaattact ttgataaaat aaatgccttg tggtaaat
//
LOCUS       DASRMS010000068         9705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_418994, whole genome shotgun sequence.
ACCESSION   DASRMS010000068 DASRMS010000000
VERSION     DASRMS010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 9705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9705
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_418994"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VFC74_03080"
     CDS             complement(<1..582)
                     /locus_tag="VFC74_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HZK24354.1"
                     /translation="MPITIAGAVQQKKIAVGKQVSLTNSHADLTCRAKVVDVLVKTLL
                     LQVTTPDMRQLKALKPQSEVIISFTVPDDAIYACQATIASFNEDAALIQIKQATPLER
                     REQRLDYRLKTAEIIYILAEQPDSPLGQDWHQACLLDISRGGASILTSVALPIGTEIS
                     VWIPLDEVDHVLEATAQVVRLKQGEEGQAIAGVS"
     gene            complement(791..1564)
                     /locus_tag="VFC74_03085"
     CDS             complement(791..1564)
                     /locus_tag="VFC74_03085"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZK24355.1"
                     /translation="MRKSFLGILTAVLVLLMAGQALASHVVQPGETLWKISQHYNTTV
                     AALAKENSLSNVNHLEVGQVLAIPGAGAQVVHTVQPGETLWKISQHYKTTVAAIAAAN
                     KIANVNTINIGAKLVIPGAKAGQTNLPDRSGRTFSASELDLLARLVQAEAGGESYQGQ
                     VAVAATILNRLGSPRFPNTISGVIYQVVNGYYQYSPVLDGRISQPAADSARRAATEAI
                     NGADPTSGATGFYNPRKTTNQWVRQQPVTVTIGNHVFFK"
     gene            1620..2126
                     /locus_tag="VFC74_03090"
     CDS             1620..2126
                     /locus_tag="VFC74_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24356.1"
                     /translation="MASKPAIPGNKRKLLSNLNNLLSVLYYSACFYSLVNMTFLLLLF
                     TVKAGYVKLTLQTYVRFGGKKMSHARFPSTHLILPEFRQVALKPTVIPEVIQREKNKQ
                     EFQRLLDISLRSKVVLHIISTNEAGSLKTTGIVKTIDHNSGLLLIQTLEGPRRIQSHE
                     VQDVLAEV"
     gene            2298..5255
                     /locus_tag="VFC74_03095"
     CDS             2298..5255
                     /locus_tag="VFC74_03095"
                     /inference="COORDINATES: protein motif:HMM:NF020337.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HZK24357.1"
                     /translation="MDSTLSQPPSRLGKKIQAKHLLLMFFVLLMFLLIFCRNIFGSHS
                     LYINEVMSSNQCTIPDEDGDYADWCELYNAGTTSLDLEGYWLSDDPTNPLRWEFPAVT
                     IAPGEYLLIFTSGKDRSDPAGPYLHTNFSLSEGETLIVSSPEMRPIDSVELGPLYANV
                     SWGRHPRSSDLWLYYLTATPGVANATLGYEQFTREPAKNINVQINEFMTTNRTSIMDL
                     DGDLSDWIEFYNAGDTEQNLEGFWLSDSSDDPFKWRFPEIILQPGEYLLVFASGKNST
                     ADKHNLHTNFKLNQTQEQLVFSTAEGHSIEEIDFSQMIANVSYGRSPDSTAWFYYPLP
                     TPGRGNETQGHKNLTGTTLPLSYQLHLNEVQTMNRSTLHDEDGDFTDWIELYNAGTSP
                     IDLAGFGLSDQEAEPFRWKLPAVTMEPQSYLLVFASGKDRRTPAKNLHTNFKLNLSGE
                     TILLTAPSGERVDSLVTGRLESGLSAGRYPDGANGRHFFMQPSPGASNTGTPLTGRAP
                     EPVFSTPGGFYDDTITLTLAPSLSLAGAVIRYTTNGQEPTANSPLYKSPLSLNKTTII
                     RAKCFANGLLASKTANNSYFIKENTPLTVISILIDPQDLWDKKQGIYVLGSNADPEFP
                     HVGANFWQDWEKPSHLQLYETNGKLGLSTDVGLRIGGQYSRAMPQKAFNIFARNQYGS
                     DVLEYPFFPDKELTTQKAITLRQSGQDATMSKIRDCLMTSLLSETTLDYQAYRPAIVY
                     LNGEYWGIYNIRERINEYFLAYNHDLNPQQIDLLQGNTIVRAGDSKHYVAMREFVADH
                     DMSKQENYDYLQTQMDLDNFIDYWLAEIYFANTDNANIRFWREKSDAGKWRWIVYDTD
                     WGFFNVNHNTLASVTNPEGTGVGRNLYTTLLVNLLKNKDFKTKFIQRGAYHLKHTFSA
                     ERVIQRIDEMASVIEPEMPKHLKRWGGSMSEWQYHIQRLKNFAEKRPALVANHFQNKF
                     NLSSKEMEIFTAR"
     gene            5290..5970
                     /locus_tag="VFC74_03100"
     CDS             5290..5970
                     /locus_tag="VFC74_03100"
                     /inference="COORDINATES: protein motif:HMM:NF027640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HZK24358.1"
                     /translation="MWREFTELLLISGDMALNLFVSLILSFLISLGIAMLYRSTHRGL
                     NYEHSFVNTLVLLAPIVTLVMLFIRGDLVLSLGLVGSLSIIRFRTPIKDSRDMVYIFW
                     VIAVGLGCGTNNWTLVLISTLLLAIVVSLLYLFEYGRPHHTDFVMVISGTSHFLPTDA
                     ETIIKQHTMMAHMRSHEINDEHWEIIFELRFSRTQEKNATNMLQEIQALAGVTRVSLL
                     APQLALPI"
     gene            5993..6808
                     /locus_tag="VFC74_03105"
     CDS             5993..6808
                     /locus_tag="VFC74_03105"
                     /inference="COORDINATES: protein motif:HMM:NF020916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HZK24359.1"
                     /translation="MLARKEVWKTWRFELKYRLTLQQYYQFRNALVPYMEQDPYTKPT
                     GSYLVRSLYFDTYDYQAYHEKVNGDYGRIKIRVRAYTDSPVGDPPLRVELKTRWGSAM
                     EKYSCFTTATIYNQFMHTHHWPEDETNPVLIEFARLYHLRTLRPQLLVQYQRQGFKAR
                     DNSNLRITFDSYVSSASAETIFPEEPFLRAQQPHEIILEIKCQKDHPRWLSQLVRQHG
                     LKLQANSKYARGIEISRPDVVTPRWSQGYYDHQAASSAMLSKHQQRSLSKFDG"
     gene            complement(6877..7002)
                     /locus_tag="VFC74_03110"
     CDS             complement(6877..7002)
                     /locus_tag="VFC74_03110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24360.1"
                     /translation="MMSFFGGFGGSGGSDNNSDFLLLIVLIIIFMSRDDNHHHKR"
     gene            complement(7183..7680)
                     /locus_tag="VFC74_03115"
     CDS             complement(7183..7680)
                     /locus_tag="VFC74_03115"
                     /inference="COORDINATES: protein motif:HMM:NF042527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6591 domain-containing protein"
                     /protein_id="HZK24361.1"
                     /translation="MKKVLIGCLVIILILSITGCGVKDKVKEKVGEEIAEKIVENAGG
                     GDVEIEGEKVTITGESGEEFSFGGADWPTSALAKNIPEFRKGKVFTVLEMNDSLLIGL
                     EEVAREDFENYLEEIKKTFTEQVYDMQSEDGVSYGAENSEGIGVMLVYTGETLNITVR
                     EAEKE"
     gene            complement(8113..8328)
                     /locus_tag="VFC74_03120"
     CDS             complement(8113..8328)
                     /locus_tag="VFC74_03120"
                     /inference="COORDINATES: protein motif:HMM:NF022659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3006 domain-containing protein"
                     /protein_id="HZK24362.1"
                     /translation="MKAVIDRFEGEYAVVLFGNKEIQVDVPKEMLPAKAQEGSWLNVS
                     FELDANATEKQAEKIQSLLEKLKNKHK"
     gene            complement(8400..9305)
                     /locus_tag="VFC74_03125"
     CDS             complement(8400..9305)
                     /locus_tag="VFC74_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK24363.1"
                     /translation="MNKEKVMAAFLIALLLLLTIGCAALPRYGEQAAQQPGTVADDMP
                     PAATAPTGQMKVHFIDVGQGDSIFIQTPQQNILIDGGERGNTVVDYLTGSNVNSLDLV
                     ISTHPHADHIGGLINVLQKISVKEVIDPGVVHTTQTFADYLTLIDEKDIKYTEGRAGM
                     QRELGGAQMQILHPSDPSSSNLNEASIVVKITFGQVSLLLTGDVGQSSLTQMLSSNYH
                     LKSTILKVSHHGSHTGISAAFLTAVTPQAAVIMCGKGNSFGHPHHETLSLLAAGKVDI
                     YRTDLQGTIVITTDGSTFAINNTTY"
BASE COUNT         2701 a         2311 c         1890 g         2803 t
ORIGIN      
        1 agaaacaccc gcaatcgctt gtccttcttc cccctgcttc aaacgcacta cttgcgctgt
       61 agcttccagg acatgatcaa cttcatctaa aggaatccac acactgattt ctgtgcctat
      121 cggcagggca actgaagtta aaatacttgc ccccccgcgg gaaatatcca gcaaacatgc
      181 ctgatgccag tcctgtccca gtggactatc tggctgttcc gcgagaatat aaataatttc
      241 tgctgttttt aaacggtaat caaggcgctg ctctcgccgt tcgagcgggg tagcttgttt
      301 tatctgaatc agagcagcat cctcattaaa actagcgatt gtcgcctgac aggcatagat
      361 agcatcatca gggacagtaa aggaaatgat gacttcactt tgcggcttca gagctttaag
      421 ctggcgcata tctggagttg tgacctgtaa aagaagagtc ttcaccagta catccaccac
      481 ttttgcccgg caagttaagt cggcatgact gttggtaagc gatacctgct taccaacagc
      541 tatttttttt tgttgaacag ctcctgcaat agtaatcggc acggcactgt aatcccccta
      601 aacaacttaa ttttaaaaaa actataaggc aaattaccaa gctcattcta ttaattacta
      661 attctcgact gttttgcact ttccttcctg ttcaactcac actattgata aataattatc
      721 ttacggcgtt aaaattcaac ctatggaaaa tagacagcaa aaagctgaag ctctcgcttc
      781 agcttttgtg ttatttgaag aagacatggt tgccgatggt aacagtaaca ggttgctgcc
      841 ttacccactg attagtagtt ttacgagggt tatagaaacc ggttgccccc gaagtaggat
      901 ctgcaccatt aatagcttcc gttgcagccc gacgggcact gtcagcagca ggctgactaa
      961 tacggccatc aagcactgga ctatattggt agtatccatt cacaacctgg tagattacac
     1021 cagagatagt gttaggaaaa cgagggctgc ctagacgatt gagaattgtt gctgccacag
     1081 ctacttgtcc ttggtaagac tcaccgcctg cctcagcctg tactagtcgc gctaataaat
     1141 ctaattcact ggcagaaaat gtccgaccgc tgcggtcagg caggtttgtt tgcccggctt
     1201 ttgctccggg gatgacaagt ttagcaccaa tgttaattgt attaacatta gctattttgt
     1261 ttgcagccgc aatcgcagct actgtcgttt tgtaatgttg cgagattttc cataacgtct
     1321 ctccgggctg cactgtatgc acaacctgtg cacctgcgcc tggtatcgcc aaaacttgtc
     1381 ccacttcgag atgatttaca tttgatagac tattttcctt ggcgagtgcc gccaccgtgg
     1441 tattgtaatg ttgcgaaatt ttccacaacg tttccccggg ctgtacgaca tgacttgcca
     1501 gcgcctgtcc tgccattaac aagactaaaa ctgcagttaa aatccccaaa aaacttttcc
     1561 tcaaagttat gtacctccca tataatatgt tatgtaaacc attatatagg ggggaggtca
     1621 tggccagcaa acctgctatc ccggggaata agcgcaagtt attaagtaat cttaacaatt
     1681 tgttaagcgt tctttattat tcagcctgtt tttatagctt ggtaaatatg accttcctgt
     1741 tactactatt tacagttaaa gcggggtatg ttaaacttac cttgcaaaca tatgttcgat
     1801 ttggaggtaa aaaaatgagc catgcccgct ttccttccac tcacctgatc ttgcccgagt
     1861 ttcggcaagt ggcccttaag ccaacagtta tcccagaagt aatccagcgt gaaaaaaaca
     1921 agcaagaatt tcaacgccta cttgacattt cactgcgcag taaagttgtt ttgcacatca
     1981 ttagtacaaa cgaagccggt tcccttaaaa caacaggaat tgttaaaact attgaccata
     2041 actctggcct tctcttgatt caaactttag aaggtccacg acgcattcaa tcccatgaag
     2101 tacaagatgt tttagcagaa gtctaatctt ctgaccccat tatccggaaa tgaacgtgct
     2161 aattattaaa atttattaat tttcactttt tcagtaggaa ttaggggggt taaattgaat
     2221 atgttgtatt aatatgtctg ttttagaaat cctttgttgg tccaacaaga taagcaacat
     2281 ttgctaagag gtgtaccatg gattctactt tgtctcagcc accaagcaga ctgggtaaaa
     2341 aaattcaagc aaaacatctg ctccttatgt tttttgtgct gctgatgttc ttgcttattt
     2401 tttgccgcaa tatttttggc agccattctt tgtacattaa tgaagtcatg agcagcaatc
     2461 agtgtactat ccctgatgaa gacggggatt atgccgattg gtgtgaactg tataatgctg
     2521 gcactaccag tctagaccta gaaggttatt ggctttcaga cgatcccact aatcccttaa
     2581 ggtgggaatt cccagctgtg actattgccc cgggagagta tctattaatt tttacatccg
     2641 gtaaggatcg cagcgacccc gcaggacctt atttgcatac caacttcagt ttaagtgagg
     2701 gtgagacgct cattgtaagt tcgcccgaaa tgcgtcccat tgacagtgtg gaattagggc
     2761 cgctttatgc caatgtctct tggggtcgtc accccaggtc aagcgatctg tggctttact
     2821 atcttactgc caccccgggt gtcgctaatg ccacacttgg ctacgaacag tttaccaggg
     2881 aacctgccaa aaatattaat gtgcaaatta atgaatttat gacaacgaat agaacatcaa
     2941 tcatggatct tgatggagat ttatcggact ggattgaatt ctataatgct ggcgacacag
     3001 aacagaatct agaagggttt tggttgtcag atagttcaga tgaccctttt aaatggcgtt
     3061 tcccggaaat tattctgcag ccaggtgaat atctgctggt ctttgcttca ggtaaaaaca
     3121 gtacagctga caagcataat ttacatacca attttaaatt aaatcaaaca caagaacagt
     3181 tggtctttag tactgccgag gggcacagta tcgaggaaat agacttctca cagatgattg
     3241 cgaatgtctc ctatgggcgg tcaccagact ccacagcatg gttctactac ccgctgccca
     3301 cccctggccg aggcaatgaa acacagggac acaaaaattt aaccggcaca actttgccgt
     3361 taagttatca attacatctt aatgaagtac aaactatgaa ccgcagtaca ctacatgacg
     3421 aagatggcga ttttactgac tggattgaac tctataacgc tggtactagc cccattgatt
     3481 tagctggctt tggcttgtca gatcaagaag cagagccttt ccgctggaag cttcctgcag
     3541 taaccatgga gccgcaaagt tatttgttgg tctttgcttc gggtaaagat cggcgtaccc
     3601 ccgccaaaaa tctgcacacc aatttcaagc ttaatctaag tggcgaaact attttactta
     3661 ccgcgccctc tggtgaaaga gtcgattctt tagtaacagg taggctggaa agcggcctgt
     3721 ctgccggacg ctatcctgat ggtgccaacg gcaggcattt ttttatgcag ccgtctcctg
     3781 gtgccagcaa tactggtacc ccgctgacgg gcagagcacc agagcctgtc ttttcaacac
     3841 caggcggctt ttatgatgac accatcacct taacgcttgc ccctagcctt tctctagcag
     3901 gtgccgtgat tcgctacacc acaaatggtc aggaaccaac agctaattcg cccttataca
     3961 agtcaccgct tagtctgaac aaaacaacaa tcattagagc aaaatgcttt gctaatggcc
     4021 tgctcgccag caagacagcg aacaattcct attttattaa agaaaacaca ccgcttactg
     4081 tcatctccat cctcatcgat ccgcaggatt tatgggataa aaagcaggga atttatgtcc
     4141 ttggcagcaa tgcagatcca gagttcccgc atgtgggtgc taacttctgg caagactggg
     4201 aaaaacccag ccatctgcaa ttatatgaaa ctaatggcaa acttggctta agtactgatg
     4261 taggcctacg catcggcggt cagtacagtc gtgccatgcc acaaaaagct tttaatattt
     4321 tcgcccgcaa tcagtatggc agcgatgttt tagaatatcc ttttttcccg gacaaagaac
     4381 ttactacaca aaaagctatt acgctgcgac aatctgggca ggacgctacc atgagtaaaa
     4441 ttcgtgattg cctgatgacc agcttactta gtgaaacaac attagattat caagcttacc
     4501 gtcctgccat tgtttatctt aacggggaat attggggcat ttacaatatt cgtgaaagaa
     4561 tcaatgaata ctttcttgcc tataatcatg atctcaaccc acagcaaata gacttgttac
     4621 aaggcaatac cattgtacgt gccggcgaca gcaaacacta tgtagctatg cgtgagtttg
     4681 tggcggacca tgacatgagt aaacaagaaa actacgacta tttgcagaca caaatggacc
     4741 tagataattt catcgattat tggcttgccg aaatctactt tgctaacacc gataatgcca
     4801 atattcgctt ttggcgggaa aaaagtgatg caggtaaatg gcgttggatt gtttatgata
     4861 ctgattgggg tttctttaat gttaatcaca atacacttgc ttctgtcacc aatcctgaag
     4921 gtaccggcgt tggccgtaat ctctacacta cccttttggt taacctttta aaaaataaag
     4981 acttcaaaac aaagtttatc cagcgcggtg cttatcatct taagcatact ttttctgccg
     5041 agcgtgtaat ccaacgcatt gacgaaatgg ccagtgtgat cgaaccagaa atgcctaaac
     5101 atcttaaacg ttggggaggg tccatgagtg aatggcagta tcacatccag cggctaaaga
     5161 attttgccga aaaaaggcca gcccttgtag caaaccattt ccaaaataag tttaacttaa
     5221 gttctaaaga aatggagatt ttcactgcta ggtaataaat agtgatttaa tcacgaaagg
     5281 agacaaacta tgtggcgtga atttactgag ttactactaa tctcaggcga tatggcatta
     5341 aacctatttg tttcacttat tctttctttc ttaattagct taggaattgc catgctctac
     5401 cgcagtaccc atcgcggttt aaattacgaa cattccttcg ttaacaccct cgtgcttttg
     5461 gctcccattg taaccttggt gatgttattc attcgaggtg atttagttct ctccctgggt
     5521 ctggtaggat cactttccat cattcgcttc cgtaccccca ttaaggacag ccgtgatatg
     5581 gtttatatct tctgggtcat tgcagtaggc ttaggctgcg gcactaataa ctggacctta
     5641 gtcttaatct ccaccctgct gttggccata gttgtctccc tgctttacct ctttgaatac
     5701 ggtcgtccgc atcacacaga ctttgtgatg gtcatttccg gtacctctca ctttctgccg
     5761 accgatgccg aaacgattat taaacagcac accatgatgg cacatatgcg ctcccacgaa
     5821 attaacgatg aacactggga aattattttt gaattacgtt tctcgcgcac acaagaaaaa
     5881 aacgccacta acatgctgca agaaattcaa gctctggctg gagttactcg cgtctcctta
     5941 ctagcgccac agttagctct accgatttaa cagtcaaggg agatgaaact agatgcttgc
     6001 ccgcaaagaa gtctggaaaa cctggaggtt cgaactaaag tatcgtctga cactgcagca
     6061 atactatcaa ttccgtaatg ccctcgtccc ttatatggaa caggatcctt acactaaacc
     6121 aaccggctcg tacctagtgc gcagtcttta ctttgatacc tatgactacc aagcttacca
     6181 cgaaaaagtt aacggtgact atggtcgcat caaaattcgt gtacgtgcct ataccgactc
     6241 ccctgtcggc gacccaccgt tacgtgtaga attaaaaaca cgctggggct cagccatgga
     6301 gaaatatagc tgctttacca ccgccactat ctataatcaa tttatgcaca cccaccattg
     6361 gccagaagat gaaactaatc ctgtgctcat agaatttgcg cggctctatc acttaagaac
     6421 actgcgtcca cagctgctgg tgcaatatca acgccaaggt ttcaaggcca gagacaatag
     6481 caacctccgt attacctttg acagctatgt ctccagtgcc agtgcggaaa caattttccc
     6541 cgaagaacct ttcttgcgcg cacaacaacc acacgaaatt attctggaaa taaaatgcca
     6601 aaaagaccac ccccgctggc tgtcacaatt agtcaggcaa catggcctta aactacaagc
     6661 taatagtaaa tatgcccgcg gcatagaaat ctcccgtccc gatgtagtaa ctcccaggtg
     6721 gagtcaaggc tactatgatc atcaagctgc ttcctctgcc atgctcagta agcaccagca
     6781 gcgctcctta tcaaaatttg atggttaggc tagaatttaa ctacaacaac agcaacgcct
     6841 ctggtcttat caccagaggc gttgctgttg taaactttaa cgcttgtgat gatgattatc
     6901 atcacgcgac ataaaaataa ttatcaaaac tattaaaagc aggaaatcac tattgttatc
     6961 agagccgcca gaaccaccaa acccgccaaa aaaggacatc atcccacctc cttgattatg
     7021 acaaccaccc ttaaaataag gttggttacc gtatgtaaac agcccctaca gtttacaaga
     7081 gtggtcacgt cacatcatac taggtggtca acgctaactg ctgcctttac attatatgct
     7141 tgctgataga tcctgtgcat ctaataaaaa attggccctc tcttactcct tttccgcttc
     7201 tcttaccgtg atgttaaggg tttcgccagt ataaacaagc ataacgccaa tcccttcgct
     7261 attttccgca ccatagctca cgccgtcttc cgactgcata tcataaactt gttcagtaaa
     7321 ggtcttctta atttcctcta agtaattctc aaagtcctct cttgctactt cttccagacc
     7381 gataagcagt gaatcattca tttccagaac tgtgaaaact ttgcctttcc taaattccgg
     7441 aatatttttt gccagtgcag aggttggcca atcggctcca ccaaaggaaa attcctcgcc
     7501 gctttctccc gtaatagtca ccttttcccc ttctatctct acatcgccgc cgccggcatt
     7561 ctcaactatt ttttccgcga tctcttctcc gaccttttcc ttcactttgt cttttacacc
     7621 gcatccggta attgacaaga tcagaataat gaccaaacaa ccgattagaa ctttcttcat
     7681 ctcatctctc ctcacaattt aagcacagct attttacagc tgccctcttt ctgatacaca
     7741 agtttatcat tgctattaaa attgcctgct tgattccact ttcattataa gaaaaatatt
     7801 tccctttgta atcatcccag gggtgattgt caattcacaa tagaggtgat tttaggggtg
     7861 atttctaatg atctgccaaa tgccttctaa aaaagtcagc tgcagatatc tccgtgcagt
     7921 ggtggcagcc tggaagcgcc aaaaagagtc cgcggcaaaa cccaagtttc tataatcaac
     7981 atcctgcctg cgttgctgct gggttagtac ataatactgt atccggagaa aaaagaactc
     8041 caggtacagg cgtattccgc tctgtagcta gagtttgatt taggtcacaa gaacctgcca
     8101 gctttgttat gattacttgt gcttattctt taacttttcc agtaggctct gtattttctc
     8161 tgcctgtttc tctgtggcgt tagcgtccaa ctcaaaagaa acattaagcc aactgccttc
     8221 ctgcgccttt gcagggagca tttcttttgg tacgtcaact tgtatttctt tgttgccaaa
     8281 caggacaaca gcgtattcac cttcaaaacg atcaattact gctttcatca tcagcctcct
     8341 tactagcgag cgttttgcaa acacaccaca gattgatcag cagcgttgag ccgcctaaac
     8401 tagtaagtag tattgtttat cgcaaatgtg ctgccatcgg tagtaatcac gatggttccc
     8461 tgcagatcag ttctgtagat atcaactttt cctgctgcta acagtgataa ggtctcatgg
     8521 tgaggatgac cgaatgaatt tcctttacca cacataataa ccgcggcttg aggcgtaaca
     8581 gcagtgagaa aagcagcaga aatgcctgta tgactaccat ggtgacttac cttgagaata
     8641 gtactcttta aatgatagtt actgcttaac atttgcgtga gacttgattg tcccacatca
     8701 ccggttagca gcaaactaac ctgaccaaac gttattttaa caacaattga ggcctcattt
     8761 aaattagaac tggacggatc agagggatga aggatctgca tttgggcgcc accgagctct
     8821 cgttgcatac cagctctgcc ttcggtatat ttaatatcct tctcatcaat taaagtcagg
     8881 taatccgcaa aagtttgcgt agtatgtaca accccgggat caataacctc tttaactgaa
     8941 atcttctgca gtacatttat cagaccaccg atatgatcgg catgggggtg cgtactaatc
     9001 actagatcca ggctgttgac attactgcct gttagataat caacaacagt attgcctctt
     9061 tctccaccat caattaagat gttttgctga ggcgtttgga tgaaaatact gtcaccttgg
     9121 ccaacatcga taaaatgtac tttcatttga ccagttggag ctgtagcagc aggaggcatg
     9181 tcgtcagcga ctgttcctgg ttgctgggcg gcctgctcac cgtagcgggg caacgcagca
     9241 caaccaatcg ttaatagcaa tagtaaggcg attaaaaagg ctgccatgac tttttctttg
     9301 ttcatgctca tcctccatat taattatatg tcttatttct acattaaaat gaaactggta
     9361 ctgccagcat gcctctaaag gatttgcctc cacataattt tcctattcat ttgtcaataa
     9421 ttgctgccat cagcaagcac aacctgataa ttagtaaatc cctcctttaa ttactaatta
     9481 attatcacac tcccttaaat tttggtcaag cacatctatc cccgcatcct aatattcttt
     9541 gccattttga catgaatttc attagcattt tttcaaaact tacggtatta ttagagtagg
     9601 ccatcggtta aaactccttt ctggtttgct tttcacttac cattttcgga gttaccgatg
     9661 gtttttcctt ttttactttt gcgaacttaa ttgtacacta tcttc
//
LOCUS       DASRMS010000069         2557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_553285, whole genome shotgun sequence.
ACCESSION   DASRMS010000069 DASRMS010000000
VERSION     DASRMS010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553285"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="VFC74_03130"
     CDS             complement(<1..1127)
                     /locus_tag="VFC74_03130"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK24364.1"
                     /translation="MKIKKTVAAFLALVMVLGSTAAIFAKDWSGQQSFSDVKKSHWAH
                     GYVENLANQGIVSGYGKTDSFKPENPILRQEAAKMIAEAAGLKASAGFVSKFVDLGDA
                     GEWALKYIYALEESGVINGFGATGEFRPKDFIQRGHIALMLVRAFNLKSGPLDVLISD
                     LNLTGNDREARAAIEILASNGIVSGFGNTNKFHPLNDIDRAGFAKMVALAQAAAAVQK
                     AEASGNPADIAGAKALIGNLPDNQDKETQKSLLDRLDETVPVKSVVVSPTTLALTVDQ
                     TGTVTATVAPGDATNKTVAWTTSNAAVATVAGGVVTAVSAGAATITATAGEKTATVAV
                     TVKNIQQAAPAGLAAVPPTAAGGNDGKITGVTDAMEYKLSTA"
     gene            complement(1420..>2557)
                     /locus_tag="VFC74_03135"
     CDS             complement(1420..>2557)
                     /locus_tag="VFC74_03135"
                     /inference="COORDINATES: protein motif:HMM:NF019192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK24365.1"
                     /translation="AARQVLSDNNATEAEINAAADALVAAMLAQNPAVLNPIIIGAAD
                     LRIPTGTALDLLEGVTALDVFGNDLSDRITADPETLDTSIAQTREVIYSVTDNYNRTA
                     IKTITIEVYKPEVLKGLLEGAIAEAKAINLSLYTSSTAAILQAAITAADEVLADDEAT
                     QEQVDSAAAALTAAINALIFKPAVGPTPPGPPAEDEPVSPSFSDVTPDDWSFEYIESL
                     YALGIVSGYGESGEFRPYNNLNRQEAAKMIAIAAGKEASKGFVSKFTDVKVNSWAFEF
                     IAALEKHGVVSGYKGTAEFRPLKNIKRSHVSKMVVIAFGLDYGDVKKTFSDIQGADCE
                     EFIKILASNGIVDGYKGTSIFKPNDEISRAEFAKIIDLAMKVGG"
BASE COUNT          604 a          644 c          576 g          733 t
ORIGIN      
        1 gctgttgaca gcttatattc catggcatca gttacaccgg taatctttcc atcgttgccg
       61 cccgctgctg tggggggcac agctgccagg cctgccggag ccgcctgctg aatatttttt
      121 acggtaaccg caactgttgc ggttttttca ccggcagttg ccgttatcgt tgcagctcct
      181 gccgacaccg ctgtcactac tcctccggcg acagttgcta cggctgcgtt acttgttgtc
      241 caggcaacgg ttttgtttgt tgcgtcgccg ggtgcgaccg tcgcagtgac agtgcctgtc
      301 tggtctacgg tcagagctaa tgttgtcgga ctaacaacaa cgctttttac cgggacagtc
      361 tcatcaagtc tgtccaggag gcttttttga gtctccttat cttgattgtc aggcagattg
      421 ccaatcagag ccttcgcccc ggcgatgtca gcagggttac cgctggcttc tgctttttga
      481 actgctgcag cagcctgggc gagagctacc atcttagcaa atccggctct gtctatatca
      541 ttaagcgggt ggaatttatt ggtattgcca aatccgctga ctatgccatt gctggccaaa
      601 atctcaatag ctgcgcgagc ttctctatca tttccggtga gattaagatc ggagatcaaa
      661 acatccagcg gaccgctctt aagattaaat gcgcgtacga gcatcagagc gatatgtcct
      721 ctttggatga aatcttttgg tctgaactct cctgtggcgc caaagccgtt aataactccg
      781 gactcctcca aagcatagat atacttcagg gcccattcac cggcgtcacc caaatcaaca
      841 aatttggata cgaaaccggc gctggctttg agacctgccg cttcagcaat catttttgct
      901 gcttcctgtc ttaaaatagg attttcaggc ttaaagctgt cagtttttcc atagccactg
      961 acaattccct gattagcaag gttttccaca taaccgtgag cccagtggct tttcttaaca
     1021 tctgagaaac tctgttgtcc cgaccaatct tttgcaaaaa tggcggcggt acttcccaga
     1081 accataacaa gcgctaaaaa tgctgcgact gtttttttaa tcttcattac ctaacacctc
     1141 accgtttctt tagtcataaa aaaactactt tttttctgct ccacgcagac actaaaacgc
     1201 caaaataacg tcttatatac ataggtacag aaaactataa aactacgcac cctcgagaaa
     1261 agaatatttt atggcaaatt cattggtgga aatgtttata ttagtatgct ttaaggactt
     1321 gtgctgatga aggcgcccaa aacaaagaac acgatgctaa gccgagagcg ccaaatccac
     1381 gatatgacgt cattatgcct tgtcgtcatg tagtaactgt tatccgccta ctttcatagc
     1441 cagatcaatt atttttgcga attcggctct gctgatttca tcgttaggtt taaatatact
     1501 tgtcccttta taaccatcca cgatgccgtt gctggcaagt atcttgataa actcttcgca
     1561 gtcagcgcct tgaatatcgc tgaatgtctt tttaacatca ccatagtcaa gcccgaaagc
     1621 tatgaccacc atctttgaca catgactgcg cttaatgttc tttaatggcc taaactcggc
     1681 agttcctttg tatccggaga cgactccatg tttttccaga gcggcgataa attcaaaggc
     1741 ccaggaattt actttcacat cagtaaactt ggagacaaaa cccttgcttg cctctttgcc
     1801 ggcagcgatc gctatcatct tggcggcttc ttgcctgttc aagttattat agggtctaaa
     1861 ttcaccgctt tcaccgtagc cgctgactat tcccagagca tagaggctct cgatatactc
     1921 aaaagaccaa tcgtcaggag ttacgtccga aaaagatgga cttaccggct catcttcggc
     1981 gggcggccct ggcggagtgg gtccaacggc cggcttgaaa atcaacgcat ttatggcagc
     2041 tgtcagagca gcggcagcgc tgtcaacttg ctcctgcgtg gcttcatcat ctgccaggac
     2101 ctcatctgca gcagtaattg cagcttgcag gattgcagcg gtgcttgaag tgtataaact
     2161 cagattaatt gctttagctt cagctatagc gccttcaagt aatcctttta aaacttccgg
     2221 tttgtaaacc tcgattgtaa ttgtttttat ggctgttcta ttgtaattgt cagtgacgct
     2281 gtagattact tcccttgttt gggcaattga tgtgtcgagc gtctccggat ctgccgtgat
     2341 ccggtcgcta aggtcattgc cgaacacatc aagcgctgtg acaccttcca gaaggtcaag
     2401 cgcggtgcct gtaggtatgc gcagatctgc agctccaatg atgatcggat ttagcacggc
     2461 cgggttttga gctaacatag cagcgaccag cgcgtcggcg gcggcgttga tttctgcttc
     2521 tgttgcattg ttgtcagata agacttggcg ggcggcg
//
LOCUS       DASRMS010000070         1877 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_286108, whole genome shotgun sequence.
ACCESSION   DASRMS010000070 DASRMS010000000
VERSION     DASRMS010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286108"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="VFC74_03140"
     CDS             complement(<1..400)
                     /locus_tag="VFC74_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24366.1"
                     /translation="MWYSQGSITATKNNTVITGVGTGFLQGVRVGDGIAIAGSSSLHE
                     VVSIASNTQLTIKPAYAGTTGSGKQYAVAPILGYDKDLSDAFNQLRLQFGDQLANLQP
                     WASASTLDDAKEQLGISSLSENKADKVHTHT"
     gene            complement(418..1047)
                     /locus_tag="VFC74_03145"
     CDS             complement(418..1047)
                     /locus_tag="VFC74_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24367.1"
                     /translation="MSFGFQLFDENGGLALDDKSFSIVGRDMVLGGKGSFPLYPEFIA
                     LGVSGAQLVGKGIIWEINGNSVVYEVLDGKTLTAVVFDDSSKSVAYPVMGLEIFNESG
                     LKTFSSGDRHFEIISASSTRGGTGGAHREPLDRGVIYILHSVFWYVIGLFAVGAGVNG
                     AVLGMDYLAATSTTLALGQRETWRIPVNNWAEHAPNPQYSSQIIRVRAK"
     gene            complement(1413..>1877)
                     /locus_tag="VFC74_03150"
     CDS             complement(1413..>1877)
                     /locus_tag="VFC74_03150"
                     /inference="COORDINATES: protein motif:HMM:NF020885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1983 domain-containing protein"
                     /protein_id="HZK24368.1"
                     /translation="VVASAQADTNEKLSTMWGVRMRQGSDGNWVTAGIGLGIENVDGV
                     DQSQFIVDANLFAVRDGVNGNQETVFAVQGGQTILRSALIGDATITMLKIGGDLYSTN
                     YVAGISGWKLSRDGDLEFNATYAGQGRIRLNSDGLYVYDAVNEFPRVKIGRL"
BASE COUNT          527 a          511 c          325 g          514 t
ORIGIN      
        1 tagtatgcgt atgcacctta tctgctttat tttccgataa agagcttatc cccagctgct
       61 ccttagcatc gtctagagta gaagccgatg cccaaggctg caagttcgct agctggtccc
      121 caaactgtag tcttaactga ttaaaagcat ccgacaagtc tttgtcatag cccaaaatcg
      181 gagccactgc gtattgcttg ccagatcctg tagttccagc ataggcaggt ttgatagtca
      241 gctgtgtgtt gcttgcaatg ctaaccactt catgcagtga tgaacttccc gcgatagcga
      301 taccatcgcc tacgcgcaca ccctgaagga accctgtccc tacaccagta atcactgtgt
      361 tgttctttgt cgctgtaatt gaaccttgtg aataccacat gtttgtttcc tcttagttta
      421 tttcgcccta actcttatta tttgcgaaga gtattgtgga tttggtgcgt gttctgccca
      481 gttattaaca ggtatacgcc atgtttcacg ctgacccaaa gcaagcgttg tacttgtagc
      541 cgccaaataa tccatcccta gtacagctcc atttacacca gcacctactg caaaaagccc
      601 tattacatac cagaatacag agtggagtat atatatcacc cccctatcta aaggttctct
      661 atgtgcgcca cctgtaccgc ccctcgtaga cgaggcgcta ataatctcaa aatgcctatc
      721 tccgctagag aaggttttta atccgctttc attaaaaatc tccaaaccca taactggata
      781 agcaacagat tttgagctgt catcaaaaac caccgcagtt aatgttttac catccaaaac
      841 ttcgtatacc acggagtttc cgttaatctc ccagattata cctttgccaa ccaactgcgc
      901 accagaaaca cccaaagcaa taaactctgg atataagggg aaagaaccct ttccgcctaa
      961 caccatatct ctgccaacaa tagaaaagga cttgtcatct aaagcaaggc caccattttc
     1021 gtcaaataat tgaaaaccaa aactcatttg cacaccatca ctaatgcagg gtagtcaatt
     1081 gaccagtaac cgccactctg aaaactaaca tattggatgt ttatcgtgtt accgctctga
     1141 gtccacctaa tttctgaggc tcggagaact ggagggctct ccattatgac aataacataa
     1201 taaggtggag cataattact aatagtaaaa ctgatactcc ctgtcaccct tcgtttgtgt
     1261 ataaccctca taagagggga attagagtcg aaatccacac ttccatcttc attaaaagtt
     1321 tgtaaaccaa aagccatact tatacccctt ctttttcttg ccacttatca caatatgcaa
     1381 aacaaccact aagcctgtat tttaacaatg cgctacaacc tcccaatctt aacccttgga
     1441 aactcattaa ccgcatcata aacatacagg ccatcgctgt taagtctaat tcgaccctgc
     1501 ccagcgtaag tagcattaaa ctccaagtcg ccgtccctgc ttaacttcca gccgctaata
     1561 cctgcgacat agtttgtgct gtacaggtcg ccgcctattt ttagcatcgt gattgtagca
     1621 tcaccgatta gcgcacttcg caatatagtt tgcccacctt gcacggcaaa cacagtctct
     1681 tggttgccgt tcacaccatc acgaacagca aacaggttag catcaacaat aaactgagac
     1741 tgatctactc cgtctacgtt ttcaatgccc aagccaatac cagctgtaac ccaattgccg
     1801 tcactgcctt ggcgcatacg tacgccccac atcgtgctga gcttttcgtt tgtgtcagct
     1861 tgcgcgcttg ctaccac
//
LOCUS       DASRMS010000071         5327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_830955, whole genome shotgun sequence.
ACCESSION   DASRMS010000071 DASRMS010000000
VERSION     DASRMS010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_830955"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFC74_03155"
     CDS             complement(<1..399)
                     /locus_tag="VFC74_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HZK24369.1"
                     /translation="MKSKNKKLITLRATHPSLLLLYFFSALVFSMLTLNPAYLLVSFI
                     AALSLNIFLDGAAKTLQSFRYFIPIHLLIVLANALFSGNGLTVLFYLGNRPVTLESII
                     YGFAAGIMLLTILLWFRAYQEVASSDQLMSV"
     gene            complement(432..1265)
                     /locus_tag="VFC74_03160"
     CDS             complement(432..1265)
                     /locus_tag="VFC74_03160"
                     /inference="COORDINATES: protein motif:HMM:NF025833.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HZK24370.1"
                     /translation="MNGNYKKKNRTKKLTIISLLLILILSISFLNACTPKNSSPEASF
                     YQPSSEKSKIQTHKASTNSTEKITTEKTKENVTEDPDYSKENSITSVTSESSSAVSEQ
                     VTTKKQTTKSTTTSKTSKAKTSASTKTAATTTSTTSSTTTKAATTTTATPDGKITVYL
                     TINCVNAVNANLSAAASYAPDGIMMSNKELSVKPGSTVLDVLKESGYVISASNNFLGT
                     YVSAIQGISSGAASGSGGWIFSVNGNFPNTSASNLIVTNGDRISFHYTIKTGDVPGSP
                     Y"
     gene            complement(1313..3472)
                     /locus_tag="VFC74_03165"
     CDS             complement(1313..3472)
                     /locus_tag="VFC74_03165"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HZK24371.1"
                     /translation="MKKLTKILALFVSVLMIYTLLPLQIFAQGSEGMPSEAEISESSE
                     DEPPEDSSAAEGQDQSPDDTEDDSAVMSASKQTEQTETESEPVETKSEPAETETLDIQ
                     TQAAETEEDVAALSAEATVKTRFILVGTSKDPSETENPYQIWLDIPFEITEGTNAWEY
                     TQTVLTENNYVYTETVWYNHLQKVTPPAEASGQIELEGGVTNGSSSSWMWSYNDNVDK
                     SAHEYLVQEGDVIRWYFTNDWSQEYAGHPEQFYPEAGNDINIVPDAIRPTDLTAWWPA
                     FGGAMDHNSVKELGFAIDGLTGSEVFTMQKQGGWIDSFSDPIQVGEWIYVVFSDTIYK
                     LNADGSVNASAKLNFLIDSTSRLAYADGLVLVPMKYGKVQALTADKLTTVWIADAPDQ
                     ILLDDSAGVTVPRSQQSLSSLKVANGKVYQGSCTVGWDPRSFGGTFRALSLKDGSTIW
                     EYSSNESGFYWSGAALLNGVLIVGNDRGEVFAFNAEDGSIVDKMTLPAAPAIRTNMIA
                     VGSQVYFISREDGALHRLDVNSDGSLGALKSVKFCDGGSTASPAISDGKVYAAGPGVL
                     SVIDAATMTIEKNYEFEGGAQGTPLVVKDKSGRTFVFFTINKEPGALYGVATDGDDVH
                     TVFTPDESQQNWCMASVSISEDGTLYYSNDSHTFFAIKLSEKQITDDDSTVTIPEEEL
                     PATGESSFLTHLISLSFFALAAVTLIFRNRLKKINNK"
     regulatory      complement(3616..3811)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4031..4372
                     /locus_tag="VFC74_03170"
     CDS             4031..4372
                     /locus_tag="VFC74_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011916063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZK24372.1"
                     /translation="MAQEKETNCRCDLSIIRKQMPQAEEMSRLANLFSVMGDNTRVQI
                     ISALRLSELCVSDLADLLSLSDSAISHQLRLLRQNNLVKARRDGKYIYYSLSDDHIAD
                     MYDVGKVHINE"
     gene            4407..>5327
                     /locus_tag="VFC74_03175"
     CDS             4407..>5327
                     /locus_tag="VFC74_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24373.1"
                     /translation="MSKNAFNNNDEKKLIGNDTCSTGREPQQEGIIGSACDVNSSCAC
                     CDEPEEMSRSEEKFKTVLLFVSIAIFIGGLVAKFALDSNTVSIVFMIIAWLLAGYKVM
                     ISAMKKLFSGFIMEEQFLMTVATVGAVILGEYPEAALVMILYQIGEHLEHFAVGKSRQ
                     SISDLLDIAAPYANRVTKTSIERIDPADITLGDTLEIQPGELIPTDGVVLSGYSHLNT
                     TAITGESIPLEVSEGSQVLSGSVNGDGLLHMKAETVYEESTASRVMDMVEEASNRKAR
                     SETLVRRFSRVYTPIVVVLAVLVSLVPPLLF"
BASE COUNT         1430 a         1197 c         1124 g         1576 t
ORIGIN      
        1 cactgacata agttggtcag aactggcaac ttcttgatag gcccgaaacc acagtaaaat
       61 tgttagcagc ataatgccgg ctgcaaatcc ataaattatg ctctccaagg taaccgggcg
      121 gttgcccaaa taaaagagaa ccgtgagccc attgccgctg aaaagtgcat tagccaaaac
      181 aatcaaaaga tgtatgggaa tgaaatagcg aaatgattgc agcgttttag cagctccgtc
      241 caggaaaata tttaaagata aggctgcaat aaaagatacc agcaggtaag cagggttcaa
      301 tgtcagcatg gaaaaaacca aggctgaaaa aaagtacagt agcagcaacg atgggtgtgt
      361 cgctcgtaaa gttatcaatt ttttgttttt tgatttcaaa gtcgtgtatt ccctgctaaa
      421 atttcaaatt atcaataagg tgaaccgggg acgtcaccgg ttttgatggt gtagtggaaa
      481 ctaatgcgat ctccattcgt tacgatcaga ttggaagctg atgtgttcgg aaagtttccg
      541 tttaccgaga atatccaacc gcctgacccg ctcgctgctc ctgaactaat accctgaata
      601 gcagaaacat acgtcccaag aaaattattg ctggcgctga taacatagcc actttctttt
      661 agaacatcta aaaccgtact tcccggctta acagacaatt ctttatttga catcattatt
      721 ccgtccggag cataagatgc agcagctgac aaatttgcat ttacggcatt gacacagttt
      781 atcgttaaat aaacagtaat tttaccgtcg ggagtagctg ttgtcgtagt agcagccttt
      841 gtagttgtac tgctggtagt ggaggtggtg gtcgctgcag tttttgtgct cgctgaggtt
      901 ttagctttac ttgtcttgct ggttgtcgtg ctttttgtcg tttgtttttt ggtagtcacc
      961 tgctcgctaa cagcactgct gctttcactt gtaactgatg taatgctatt ttccttgctg
     1021 taatcaggat cctcagttac attttctttg gtcttctcag tggtaatttt ctcagttgaa
     1081 tttgtagaag ctttgtgagt ttggatttta cttttttctg aggatggttg ataaaagctg
     1141 gcctccggag aactgttttt tggagtgcaa gcattcaaaa aagaaatact caagattaat
     1201 atcaagagca aactaataat agtcagtttt ttggttctgt tttttttctt gtaattaccg
     1261 ttcatcttat gaacccctta agcccgctgt tcagcgggct taatcatgtc ttttatttat
     1321 tatttatttt tttcagtctg ttccggaaaa taagagtcac agctgctaaa gcaaaaaagc
     1381 ttaaactgat aagatgagtc aagaaactgc tctcacctgt agcaggtaat tcctcctccg
     1441 gtattgtcac agttgaatca tcatctgtaa tctgcttttc tgacagtttt atcgcaaaaa
     1501 aggtgtgtga gtcattgctg taataaagtg taccgtcctc tgaaatggat acggaagcca
     1561 tacaccagtt ttgttgtgac tcatcaggtg tgaataccgt gtgaacgtca tctccatcgg
     1621 tagctactcc ataaagggca ccaggctctt tgtttatagt aaaaaataca aaggtacgtc
     1681 cgcttttatc tttcaccact aatggggtcc cctgagcccc cccttcaaat tcataattct
     1741 tctcaatcgt cattgtggct gcatcaatta ctgacaaaac cccgggacct gcagcatata
     1801 cttttccgtc agaaatcgca ggtgaagcag tactgccgcc gtcacaaaat ttaacacttt
     1861 tgagcgcccc cagacttcca tcgctgttta catccaggcg atgcagagca ccatcttccc
     1921 gggatataaa gtaaacctga cttcctaccg caatcatgtt tgtacgtatg gcaggagccg
     1981 caggcagggt cattttatca acgatagaac cgtcttcagc attaaaggca aaaacctcac
     2041 cccgatcgtt acctacaatc aaaaccccgt tcaacagagc agctccgctc cagtagaaac
     2101 cggattcatt tgatgaatat tcccaaattg tgctgccgtc tttgaggctg agggcacgga
     2161 aagttccgcc gaaagagcgc ggatcccagc ctactgtaca gcttccctgg taaaccttgc
     2221 cgtttgctac cttgagtgaa gataatgatt gctggctacg gggaactgtt acgcctgccg
     2281 aatcatccaa taaaatttga tcaggtgcat ctgctatcca cacagtagtc agtttatcag
     2341 cggtcagcgc ctgcacttta ccgtatttca ttggcactaa caccaaaccg tctgcatagg
     2401 ccaaacgaga ggtagaatca atcaggaaat tcagtttggc gctagcattg actgaaccgt
     2461 cagcgttgag tttatagata gtatctgaga aaactacata gatccactct ccaacctgaa
     2521 tcggatctga gaaagaatct atccagccac cttgcttttg cattgtaaaa acttcagacc
     2581 cggttagacc atcaattgca aagccaagct ctttaacgct gttatgatcc atggcaccac
     2641 cgaaggcggg ccaccaagcc gttaagtctg tgggtctaat ggcatccggt acaatattga
     2701 tatcattgcc ggcttccgga taaaactgtt caggatgacc agcatattcc tggctccagt
     2761 cattggtaaa ataccagcgg attacatcgc cttcctgcac cagatactcg tgtgcggatt
     2821 tatcaacgtt gtcattgtag ctccacatcc agctagagct tgaaccattc gtaactccgc
     2881 cttcaagctc tatctgtccc gaagcttccg caggaggagt gactttttgc aggtgattat
     2941 accataccgt ctcagtgtag acgtagttat tttctgttag aacagtctgg gtatattccc
     3001 aggcgtttgt gccttctgtg atttcaaaag gaatgtcaag ccagatttgg tacggatttt
     3061 cagtttccga cggatccttg cttgtaccga ctaaaataaa tctggtttta acagttgctt
     3121 ctgcacttaa cgcagcaaca tcttcttctg tttcagctgc ctgtgtctga atatcaagag
     3181 tttcagtctc agctggttct gatttagtct caactggttc tgattcggtt tccgtctgtt
     3241 ctgtttgctt acttgcagac atcactgcgg aatcatcctc tgtatcatca ggtgattgat
     3301 cctgcccttc agcggctgag ctgtcttccg gcggctcgtc ctctgaactt tctgagattt
     3361 ctgcctcaga cggcatgccc tccgaaccct gcgcgaaaat ttgcagcggt aataaggtat
     3421 agatcatcaa tacgctgaca aacagggcca aaatttttgt tagtttcttc atcttttaac
     3481 tctcctttta attataaggg ttgtgagaat gctatcctgt ttatcccgag acagaaaaaa
     3541 cggcagcaaa tcaatgactt gctgccgttt tacacatttc ttaatctgtt caggggaagc
     3601 aaatactcgt tgcatccttc tcctaaagca acagatcggt cttctggctt acgatccggg
     3661 tcatcccccc ttaactccac cttcccaact ttgtcagtgg ctggcatttg cccggagtta
     3721 attagatcga ttacagcggc ggcaccgcac aggactttaa cctgtttccc taacagttct
     3781 cacgaactgt atctgctctc tatgtagctt atcactttgg aagtcaaaaa gcttttgtga
     3841 ctgcattata cgatggcctc tcaagccagt caagcaaagc aatagcagag aattaaccaa
     3901 ttccttagtc atattgcctg tttttttgtt accatttttt tcaaataaat tgacatttta
     3961 ctaaagatta tttattatca atttaacact tgagcatata tggaattgaa gcaaacagga
     4021 ggggagattt gtggcccagg aaaaggaaac aaattgtcgc tgtgatttgt caataattag
     4081 aaagcaaatg cctcaggctg aggaaatgtc acgtttggct aatttgtttt ctgtcatggg
     4141 cgacaacaca cgtgtacaga taatctcagc tttgcgcttg agcgaacttt gcgtcagcga
     4201 cctggctgac ctgctatcat tgtctgattc agctatttct catcagttgc gtttattgcg
     4261 tcagaacaat ctggtcaagg ctcgccgaga tggaaaatac atctattaca gcctctccga
     4321 tgatcacatt gcagatatgt acgatgtcgg caaagtccac attaatgaat aagcataatc
     4381 tatctataag gagcaataat ttataaatga gcaaaaatgc ttttaataac aatgatgaga
     4441 aaaaattgat tggaaacgac acttgcagta caggccgaga gcctcaacag gaaggaatta
     4501 taggaagtgc ctgcgatgtc aactcttcct gtgcctgttg tgatgagcct gaagaaatga
     4561 gcagaagtga agaaaaattc aaaacagttc ttctttttgt ctccattgca attttcatag
     4621 gcggtctggt cgccaaattc gccttagact ccaatacagt ttccatcgtt ttcatgataa
     4681 tagcctggtt actagccggc tacaaagtca tgatcagcgc tatgaaaaag ctcttttccg
     4741 gctttatcat ggaagaacag ttcctcatga cagttgccac cgtcggcgca gttatactgg
     4801 gcgaatatcc tgaagctgca ctagttatga ttctttatca gataggagag catctggaac
     4861 atttcgccgt cggtaagtca cggcaatcta tttctgatct tttggacata gctgctcctt
     4921 atgccaaccg cgtcaccaaa accagcattg aacggattga tccggccgat atcactttgg
     4981 gcgatactct ggagatacag ccgggcgaac ttattcccac tgatggagtg gtcctgagtg
     5041 gttacagcca tctcaacacc acagctatta ccggtgagtc cataccgctt gaagtaagtg
     5101 aaggcagcca ggtactaagc ggtagtgtta atggtgatgg ccttttgcac atgaaggcgg
     5161 aaacggttta tgaagaatcc accgcaagtc gcgtcatgga tatggtcgaa gaagcttcca
     5221 accgtaaggc ccggtcagaa actctggtta gacgtttttc acgtgtttac acgcctatcg
     5281 tcgtcgtttt ggctgtttta gtttctttag ttccaccttt gttattt
//
LOCUS       DASRMS010000072         1270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_286742, whole genome shotgun sequence.
ACCESSION   DASRMS010000072 DASRMS010000000
VERSION     DASRMS010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1270
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286742"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..479)
                     /locus_tag="VFC74_03180"
     CDS             complement(207..479)
                     /locus_tag="VFC74_03180"
                     /inference="COORDINATES: protein motif:HMM:NF022562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyocin activator PrtN family protein"
                     /protein_id="HZK24374.1"
                     /translation="MNTLFLLMAQYNGQAIIPVDTVCADYFSHLSPKNLRQNIAHGKI
                     DLPLVQMDSSRQAARGVHVRDLAAYLDNQRQIAVSDHEKLWGRSSG"
     gene            complement(604..1026)
                     /gene="dut"
                     /locus_tag="VFC74_03185"
     CDS             complement(604..1026)
                     /gene="dut"
                     /locus_tag="VFC74_03185"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HZK24375.1"
                     /translation="MIIKLNKLHQDAKLPAYGSDGAACFDLYAVEDTRSLFGRAVVGT
                     GLSVEVPPGYEMVVRPRSGLAFKSGIHAFAGTIDSDYRGEVKVLLVTDSSSSGFEIRK
                     GERIAQAAIQPVVRVFFEESQDLSDTERGSSGFGSTGS"
     gene            complement(1023..>1270)
                     /locus_tag="VFC74_03190"
     CDS             complement(1023..>1270)
                     /locus_tag="VFC74_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24376.1"
                     /translation="TTGVDTFVVYHNELGQHFVRPLAEFGEAMNEVKKPQETEVWQGN
                     WPESADERMIPIMQNGNDGKVYNDIARQNETENKVVK"
BASE COUNT          303 a          327 c          288 g          352 t
ORIGIN      
        1 cagattgatg atctgcactt tcacgaccta aggcacgagg gcgttacaag gctgtttgaa
       61 atgggctggg atattccaag agtagcgtct gtgtctgggc acagggactg gaactcaatg
      121 cgcagataca cgcatttaag cggtgcgggt gataagtacg agggttggga gtggattcag
      181 cgcatagttg agagtaagag tattttttac cctgagctac gcccccacaa cttttcgtga
      241 tcactcactg caatctggcg ttgattatca aggtaggcgg caagatcacg aacatgcacg
      301 ccgcgcgcgg cctgcctgct gctatccatc tgcactaaag gcaagtctat tttgccgtgc
      361 gcaatgtttt gccgcaggtt ctttggtgat aagtggctga agtagtcagc acaaacagta
      421 tcaacaggaa tgattgcctg gccgttgtat tgggccatta gtaaaaacaa agtattcatt
      481 ggttgccttt tttagttagt ctctgccgtt caagtagcgc gccgcatcgt ttgcactgct
      541 gcttttcagt ggcgcactgg tcgttaggcc gccgcgtttt gcggcatatt acttttggcc
      601 tcattacgat cctgtgcttc caaagccact agatccgcgc tctgtatcgc taaggtcttg
      661 gctctcttca aaaaaaacac gcaccacagg ctgtatagcc gcctgagcaa tgcgctcgcc
      721 ttttcttatc tcaaatccag aactgcttga gtcagtaacc agtagcacct taacttcgcc
      781 ccgatagtcg ctatcaatag tgcctgcaaa cgcatgtatg ccagacttaa aagctaaccc
      841 tgaacgaggg cgcacgacca tttcatagcc tggcggcact tctacagata aaccagtgcc
      901 aactactgcg cgaccaaaca agcttctagt atcttcaacc gcatatagat caaagcaagc
      961 cgcaccatca ctaccgtaag ctggaagctt tgcgtcttgg tgcaatttat ttaacttgat
     1021 tatcacttta ccaccttatt ttcagtttca ttttgtctag caatatcgtt gtagaccttc
     1081 ccgtcattgc cgttttgcat tatcggaatc attctttcgt ctgctgactc cggccagttg
     1141 ccctgccata cttctgtttc ctgcggcttc tttacttcat tcatagcctc gccaaactca
     1201 gccagaggac gcacaaagtg ctggcctagt tcgttatgat aaacaacaaa ggtatcgaca
     1261 ccagtcgtgt
//
LOCUS       DASRMS010000073        19749 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_831626, whole genome shotgun sequence.
ACCESSION   DASRMS010000073 DASRMS010000000
VERSION     DASRMS010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 19749)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19749)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19749
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_831626"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="VFC74_03195"
     CDS             <1..190
                     /locus_tag="VFC74_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24377.1"
                     /translation="NNVKANISMEISIPLLNITTSSENCTLYTSQVTKNLRMHHKDFL
                     PNVKERAISGDSPFCWLK"
     gene            complement(202..942)
                     /locus_tag="VFC74_03200"
     CDS             complement(202..942)
                     /locus_tag="VFC74_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HZK24378.1"
                     /translation="MMLIIGVVGCSGNEAENTFTVGFDKNFPPMGFVGDDGEFTGFDL
                     ALAAEVAKRLEKELVLQPIAWDNKDKELAAGSIDCVWNGFTINGREDDYTWTDPYMKN
                     QQVFVVKADTDISTLADLAGKVVMVQVDSSAESALADKPDLVATFEDLLTAADYNSAL
                     MDLEAGAVDAVAMDEIVANYQIEKRNAKFKVLEETLAAEEYGVGFLLGNEELRDQVQS
                     TLEEMAADDTLQSISDKWFGKNITTIGK"
     gene            complement(1155..2006)
                     /locus_tag="VFC74_03205"
     CDS             complement(1155..2006)
                     /locus_tag="VFC74_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4474 domain-containing protein"
                     /protein_id="HZK24379.1"
                     /translation="MTTMPGQKSNQGTGNAGIDHAFAKVGYVYDQQQKIFFSHRQAWQ
                     RDYGYCQLYDEMLAPLSMIIDCEPIYFNYEQRRWMIEFWKGQYALTTGCEVGVYYTEA
                     ENINIPGVFNGPFYNCVGDADLLDMSCTLYKDGKKLFTRADKHWWLTGFILGEFSDPT
                     DLTLDVSITLKDEAMLAAFLEGLRSTGYQEDEYKITERSVSLTLAKPRTAQPITRTPE
                     IERIMQWKNKLLCDLYQSITNNITSVIERMEFIKENAPDLYEEIMRMGKNKQLFGQFN
                     LNLPLDS"
     gene            2109..2852
                     /locus_tag="VFC74_03210"
     CDS             2109..2852
                     /locus_tag="VFC74_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HZK24380.1"
                     /translation="MKLKHFLKSLFQIISHMELAEATALILANDGKQEIALFFNEVDG
                     ESGKKFIEFLVDGTQDADQPLSGGYILTLCHFHHPWTHRGYLVGKSSADEITAIFTTA
                     TTLWQEEKTAEAIYQLGRALHLLQDIFIPHHSGITGVKGHGELEKWLTENWLAYQTTS
                     GGYYQWEEDFYHQNGHQHHVSSTNPYDWIDYGSHLSIEWYEQYFAVGTYDENTFRTVA
                     PRIVSNALRFSAGFINFFFEQAVIAAREQ"
     gene            complement(2863..3111)
                     /locus_tag="VFC74_03215"
     CDS             complement(2863..3111)
                     /locus_tag="VFC74_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HZK24381.1"
                     /translation="MKIKVKYFGLAAEVLKPIPASLIIAESTTIGQLLEQLSKHQDQS
                     TIDALRAATFLVNKEKATRDTILLEGDEVLVMLRLAGG"
     gene            complement(3187..5046)
                     /locus_tag="VFC74_03220"
     CDS             complement(3187..5046)
                     /locus_tag="VFC74_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HZK24382.1"
                     /translation="MLPGYAGKMLFVDLAKGTWEEKELLEDLAKDYIGGYGIGAKILY
                     NLMPPGADALGPENILGFVTGPVTATKAFFGGRYTLVHKSPVTGGWNDANSGGYFGPE
                     LKKAGFDAVFLSGLSENPVYLYINDGKVEIRDAAHLWGKDTKETWAALIEETGEPKLR
                     VVAIGPAGEKVSLLACPINDGHRAPGRGGGGAVMGSKKLKAIAVRGTGVIAMVEEEKI
                     LAMNKEMVINLKNNPGSRAFGEMGTGAGTPASALNGDSPVKNWGGIGVVDFGEDAAEK
                     LGAAALDQHKVRKYNCANCPLGCGAEYVVKTGRWPLAQTERPEYETAAAFGSNMLNSE
                     ADAVLKCNEICNRYGLDTISTGGTVTWAMECYNNGVLSREELDDIDLTWGNGEAIVAL
                     TQKIADQDGCGAVLAHGSASAAKTWGKGAEFLQTVLGIELPMHDPRLAPGFARTYQYD
                     PTPARHVKGGLGFPQMFGAPIGDKYDYDDTGKLDVMLTANQEVQNCVGFCTFMAQGGL
                     QNAEIRYLEAVTGRPFTAEDSLKTGLRILNLRHAFNLREGLTPKDFVIPARCVGKPPQ
                     RSGPVADVDIDHEKLGKNFFAQVNWDFETGKPSLDSLRELGSLDEVIKDLYDE"
     gene            complement(5415..6764)
                     /locus_tag="VFC74_03225"
     CDS             complement(5415..6764)
                     /locus_tag="VFC74_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009246494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HZK24383.1"
                     /translation="MPDGDSIFWPLILQGILILFNALFALTEIAVISMNDNKMAKLAA
                     VGDKRAVRLVQLTDQPARFLSTIQVGITFAGFLGSAFAADNFSDKLVAILVNVGVNIP
                     IATLDTIAVILITLFLSYFTLVLGELVPKRIAMRKSEQIALGMSGFLYFTAQIFAPVV
                     WLLTVSTNFLLRLLGIDPLAIDDVITEEEIRMMVDAGSEKGAINYSEKEMIQNIFEFD
                     DKSAGEVMTHRTEVSLLWLDENTEQWDETISESRHSIYPLCDESIDNIVGVLNAKDYF
                     RIKGKTRDKIMQLAVKPAYFVPETVRTDVLFRNMKKSRNHFAIVLDDYGGMSGIITMN
                     DLLEELVGNLDDDSSLPQEPPLIERIDSQTWKIQGSVPLEMLSKQLGVKLPDDDYETF
                     GGLVFGLLGTIPDDGSTPELEEYGLIIKVTEIKEHRLVSALVCLEQPDTTASIEVAN"
     gene            complement(7170..7499)
                     /locus_tag="VFC74_03230"
     CDS             complement(7170..7499)
                     /locus_tag="VFC74_03230"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HZK24384.1"
                     /translation="MITISIENAAKELAKELKATTEFADLRQAQARIKLDPAAQDIMA
                     EMEQIQVNIQEMLTDGLPVDEEFQKLYQLQHQAVANTTLSQYFSAQEAFSLIMKNADQ
                     IINQELE"
     gene            complement(7563..8264)
                     /locus_tag="VFC74_03235"
     CDS             complement(7563..8264)
                     /locus_tag="VFC74_03235"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: protein motif:HMM:NF001243.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HZK24385.1"
                     /translation="MQRKQEVNTWRFFEQIADRYDLINSLISFRQHQRWRKQTMKQLT
                     LRPGQRVLDLCCGTCDWTVELAKAVGAEGKVVGLDFSQQMLVQGRAKIAQANLQNVEL
                     ICDNALQLPFSNDCFDYVTIGFGLRNVQNYQQVIKEMYRVVKPAGTAVCLETSQPSLL
                     LYKQLYHFYFKYAMPLIGKIVSNNYEAYNWLQQSTFNFLNKAELAQLFIDNGFSQVFV
                     HSFSGGVAAMHLCIK"
     gene            8533..9462
                     /gene="phnD"
                     /locus_tag="VFC74_03240"
     CDS             8533..9462
                     /gene="phnD"
                     /locus_tag="VFC74_03240"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK24386.1"
                     /translation="MPKRLLLLICILLCTVYFSGVMQSKQLPYISLWPDSLQAEKNDV
                     TPKTDTIRIAIVSSASARKSFIFYQDFVDYFEQKAGLQVELILRENYNIANGLVCEGS
                     VDLAFISSFSYVLSESNCNYSLLLRPIAKDDTPYCSCIIVRGDSDIQSLKELRGKTFT
                     FTDPLSTLGRLYPLYLLTTAGESSNTFFENVYYASSTDYALHSLDSRLVEGAAINSLV
                     LQSLNLPSKEFASRFKIIHQSEPFPLNPLVVNTNMHPDRQKILKNILLTMHEDNRGAA
                     ILHDLGYSHFVAAKPADYDRIREMVSVVKDQLP"
     gene            9459..10931
                     /locus_tag="VFC74_03245"
     CDS             9459..10931
                     /locus_tag="VFC74_03245"
                     /inference="COORDINATES: protein motif:HMM:NF028512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HZK24387.1"
                     /translation="MSESLLKRSLKRLRAAYSPGVRSKFVFLWIALVLIIGICFTWQS
                     ERITESVIRSDLHARGLSIARDVASRSADSILTHNYHELRQTINDTLANNKDIVYLFL
                     LDRQGTVIINSRQDRQPSTALRQLQTLLPEEEFKTATFRSEEGIIHDLAVPIFGGNAG
                     TVRIGITEANLQKALTKTRNILFTTLLIALVLGSFITAYFTNKLLAPLRQLTTATHII
                     REGDFSARVSIQTQDEIGHLATAFNMMAEKLSTYKLENKENRAELNRIQKLRVQLVQH
                     LITAQEEERKRIARELHDETSQSLTALKLGLKAMEQTTSTAEIQDLSHELRQMLGATL
                     NEITAISRNLRPSVLDDMGLVAALARFLGDSSVRLGKRIAFKHEGLVGKRFPVYVETA
                     IYRVVQEAVTNSLKYAHAENIFVSVTYINNLLKAIVDDNGVGFDPTTVFDGDIPQEGF
                     GLFGMQERATLIDGTLQIDSAIGRGTTITVTVANVQEAGA"
     gene            complement(11114..11899)
                     /locus_tag="VFC74_03250"
     CDS             complement(11114..11899)
                     /locus_tag="VFC74_03250"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HZK24388.1"
                     /translation="MLNSLLIDTSKCIGCRACQVACKQWNRLPAEETSFTGFYENPVR
                     FTANSWTRVSFREHEDESTGDVSWYFAKIGCMHCTDAACEKVCPAGAISHTETGAVVT
                     DPKKCIGCNYCVASCTFKVMSFNQKENISQKCTLCSDRQAIGLIPACAKACPTGAIQF
                     GTRAEMVDLAHRRVNDLVVQGSSKAQIYGLEELNGTGVMYVLKDDPEEYGLPANPKIP
                     ISAYIWNAIFRPLRIFVVALVGLLLWENKRETEKVLPTENEAK"
     gene            complement(11901..14900)
                     /gene="fdnG"
                     /locus_tag="VFC74_03255"
     CDS             complement(11901..14900)
                     /gene="fdnG"
                     /locus_tag="VFC74_03255"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012646730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(14313..14315),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HZK24389.1"
                     /translation="MKVSRRSFLKLTGAAVGSATLASFGFSNTTEAAPVPVQYAKEVT
                     TICPYCSVGCGIICYVQNDKVVGAEGDPDHPINEGSLCSKGSSIFNLAYVYDQQGQAI
                     EHPQRMKKVLYRAPRATEWKEVDWDWALTQVAQRIKQTRDSTFETVDEHGVTVNRTRA
                     IAHLGSSNCTNEENYLYHKLIRALGIINIDHCARLUHSSTVAGLGNSFGRGAMTNHWI
                     DYKNSDVFLAFGCNPAENHPISMKWVERARAEKGAKLIVVDPRFTRSAATADLYAPLR
                     PGTNIAFLGGLINYVLSNKLYHEEYVLNYTNATYLINPEFSFNDGLFSGAHDAEDRAV
                     NYDKDSWSYQVDAEGKVIKDPTATDQHSVLQLMKKHYERYDFKTVSRITGCPVDKLQA
                     VAELFASTGQPGKAGNIMYAMGVTQFTHGSQNVRAIACLQLLLGNMGIPGGGVNAQRG
                     QANVQGSTDMAMLYHLIPGYLNMPKAALHEDLAAYLEKETPAGGYWTNKPKFLISMLK
                     AWYGDYATQDNDFCYDYVPKLDTKDRSHIGMYKYMGEGEIKGMICWADNPAVTGPSAS
                     TKRNYSQNLDWLVSIDIFENETAAFWKEPGVDPTEIDTEVFLLPSSVSFEREGTIANS
                     GRWIQWRYQAQEPLGDAHTDIWIVDQLFKAVKKEYEKNAGPFAEPILKMKWDYGAEAD
                     ANAVAMEINGYDLKTGLLLEDFTKLADDGSTACGCWIYSGYYNNATHPACKSRIKEDS
                     GIGTNAKWSFAWPLNRRIIYNRCSADPQGNAWNPDTPLMWWDGLTWQRNDVPDFNANV
                     PPETTAQAPYIMLPELQARLFASGLADGPFPEHYEPAESPIRNILSTTVQFNPVTKIW
                     YEDHLAAAGDKNFPYVISTYRVSEHWQSGIMTRNTPWLNELMPALFIEISPTLAEKLG
                     IKNGDQVEISSYRKQHITALACITPRMKPLLVDGELREMVGMPFHWGFMGLNKGAVVN
                     DIVPSIGDANTNIPEYKAFLCNIRKVG"
     gene            complement(14917..15918)
                     /locus_tag="VFC74_03260"
     CDS             complement(14917..15918)
                     /locus_tag="VFC74_03260"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZK24390.1"
                     /translation="MGKDLNLQVSSAPHLHAGDQVSTAMRDVTIALMPVTLVACYFFK
                     LNVPFLILVCVVTAIFTEVIFRRAMRKEPQLQDGSAILTGVLVALSFSAATPWWLAAA
                     ATFIAVGLAKEFLGGLGWNRFNPALFGRVSVILFAPWLVYLNNEFFYWRVSFPGIDTV
                     TQATPLTMLQMGLPLPNMGTFFIATQGGGIAESSALALLIGGAYLLYKKHITWHIPVS
                     MLATVFVGTAVLGEHPIYHLLSGGLLLGAFFMATDWVTSPITKKGKIIFGVCIGIIVV
                     VFRVLLAPTEGVAFAILIMNAFVPLIDRLTRRKKFGEPVVQRVPQAVPASVKAAAKN"
     gene            complement(15942..17228)
                     /gene="rsxC"
                     /locus_tag="VFC74_03265"
     CDS             complement(15942..17228)
                     /gene="rsxC"
                     /locus_tag="VFC74_03265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HZK24391.1"
                     /translation="MAKTFERGKEVPHYKKLTENLPIQDLPQPTELIIPLKQHIGAPN
                     RRLVSVGEKVKAGQKIGDSDANISAPVHTGLSGTVREIGSRVTLSGQLEDCVVIDVDP
                     EQDACVYTEEDVSQLSPEQIRRRIREAGIVGMGGAGFPTHVKVNPPTSIDYLILNGCE
                     CEPFLTCDHRLMVEEADEMIAGARILSQVLNGAKVIFGIEENKMDAIEVVKAKLASEA
                     DMEVIPLEVKYPQGAEKQLIYATTGRVVPLGKLPAEVNVVVQNVATAKAVWEAVKFQR
                     PLTERIVTLTGAAAVQPGNYRVVIGTPLSHLLACTGGLTDRVTKVVLGGPMTGFAQET
                     LAAPIVKGCGGVLALTEELAVKTQEYYSCVRCGKCVDHCPMGLYPNYIGMYGELGRYA
                     LAKEWGALDCFECGICAFVCPSQRPIVEFVRRVKRA"
     gene            complement(17252..18154)
                     /locus_tag="VFC74_03270"
     CDS             complement(17252..18154)
                     /locus_tag="VFC74_03270"
                     /inference="COORDINATES: protein motif:HMM:NF016131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HZK24392.1"
                     /translation="MVNYPPTDEAVKQEVKFYLQEHQNMKSLLHFYEKLYAHQRGFTN
                     SLTTYSPPQTANREVMQGKPLLAELALPIPVQPTLELAQTILLDACSELLVKNEQQLN
                     VMCNLMQSTFTADNIKEFYSRPSNLSLAKVQKFLFTKHDTAELAEQQLAVTAQVIRSA
                     LQMYFVYFAQQMQAAIDASNWNEGVCPVCGEKPMLAMLRAEDGSRVLECGLCHTQWEV
                     SRIRCMSCGNTDHSKLSFFYLPDQVHRRVYVCSQCHNYLKTVVLKELARDIIPDLENL
                     MTHFLDVLAADEGYGESGDKTTFN"
     gene            complement(18408..18713)
                     /locus_tag="VFC74_03275"
     CDS             complement(18408..18713)
                     /locus_tag="VFC74_03275"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HZK24393.1"
                     /translation="MHPELQERNEYHLKMAQIEAFCTVIQTGSISQAARYCHISQPAV
                     SMQVRDLEQYCQQRLLKRSNKGVTPTPAGWVVYEYCKKIIHLKQDLKLELKQFQHTP"
     gene            complement(18833..19498)
                     /locus_tag="VFC74_03280"
     CDS             complement(18833..19498)
                     /locus_tag="VFC74_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK24394.1"
                     /translation="MCNDTIKIMLIDDHPIVRKGLHLIIRTQESMEVIGEAGSVGEGI
                     TIVQELMPDLIILDLTLPDRSGIEFIKEIKKICPQIKILVLTIHEEDDYIQQVLTEGA
                     DGYLLKKAADDELILAIRAIVRGEMVVDSSLTKRLWQRYLQPVDKKQQSKEKEILSKR
                     QKETLSLVARGYTDKQIAEQIHVSVKTVESHKARIKEKLDIVQRSEFVAYAMEHNLLD
                     LET"
BASE COUNT         5563 a         4496 c         3896 g         5794 t
ORIGIN      
        1 gaataatgtt aaggcaaata tttcaatgga gataagcatc cctttgctta atataactac
       61 tagcagtgaa aactgcacac tatacacctc ccaagtaaca aaaaacttgc gaatgcatca
      121 caaggatttt ttacccaacg tgaaagaaag ggctatcagt ggagacagcc ctttctgctg
      181 gctaaaatga ttagcttatt tttatttacc gatggtcgta atattcttac cgaaccactt
      241 gtcagaaatg ctctgtaagg tatcatccgc agccatctct tccaaagtag actgtacctg
      301 atctcttaat tcttcattgc caagtaaaaa tcctactcca tattcttcag ctgctaaagt
      361 ttcttctagt actttaaact tggcatttct tttttcaatc tgatagttag ccacaatttc
      421 atccatagct acagcatcaa ctgcaccagc ttcgagatcc attaaagcac tgttataatc
      481 agcagctgtt aataaatcct caaaggtagc tactaaatca ggcttatctg ctaaagctga
      541 ttcagcactt gaatcaactt gtaccatcac aactttacct gctaaatccg caagcgtact
      601 aatgtcagta tcagctttca ctacaaagac ctgctggttt ttcatgtagg gatcggtcca
      661 ggtataatca tcttctctgc cgttaatggt aaaaccattc cagacacaat caatactacc
      721 ggcggctaat tccttatctt tgttgtccca ggcaatgggc tgcaacacaa gctctttttc
      781 taaacgttta gcaacttcgg cagctaatgc taagtcaaag ccggtaaatt ctccatcatc
      841 accgacaaat cccataggcg ggaaattttt gtcaaaacca actgtaaatg tattctctgc
      901 ctcatttccc gaacaaccta ctacgccaat tattaacatc atcatcataa taagtgctat
      961 tgcttttctc atttatctat cccccatctt cctttagctt cttgcttcac tatattacca
     1021 cgttaatgtg ctaaagtaaa taggtaattt ttaattttgc caagctgtag ttctccccag
     1081 gatgacagca cctaaagaat aagaaaggtg cgctaaataa acaaataaag cgctcctttc
     1141 ttttgtgggg agagttagct atctaaaggt aaattaagat taaactgacc gaatagctgt
     1201 ttatttttac ccatacgcat aatttcttca tataggtctg gcgcattttc ctttataaac
     1261 tccatcctct caataacaga ggtaatatta tttgtgatgc tttggtagag atcacagagc
     1321 agtttattct tccactgcat gatacgctct atctctggtg tgcgggtgat tggttgtgct
     1381 gtacgtggct ttgccagcgt caggcttact gagcgctcgg tgattttata ctcatcttcc
     1441 tgatagcctg tgctgcgcaa accctcaaga aaggccgcaa gcattgcttc gtctttcagc
     1501 gtaattgaga catccaacgt taagtcagta gggtcggaga actcccccag gataaagccg
     1561 gtcagccacc agtgtttatc cgcccgggta aaaagttttt tgccatcttt ataaagagtg
     1621 cacgacatat ctagcagatc ggcatcgcca acacaattat aaaatgggcc attaaaaact
     1681 cccggaatat taatattctc tgcttccgta taatagactc ccacttcaca gcctgtggtg
     1741 agggcatatt gacctttcca gaattcgatc atccatcttc tttgctcata gttaaaataa
     1801 atcggttcac aatcaataat catgcttaaa ggcgcgagca tttcatcata taactggcaa
     1861 tagccgtaat cacgctgcca agcttgccga tgtgaaaaaa agatcttttg ctgttgatca
     1921 tatacatagc cgactttagc gaaagcatga tcgatgccgg cgttaccggt accctggttg
     1981 cttttctgcc ccggcatggt agtcatggta tttccctcct gtcaatatcc ttttatctaa
     2041 gatattggca agagcttaaa tttgctacag tcatatatca tttaaaaaac aactgaataa
     2101 actaaactat gaaactaaaa cattttttaa aatcattatt tcagattatt agtcatatgg
     2161 aacttgctga agctaccgca ttgatactcg caaacgacgg caagcaggag attgctttat
     2221 tttttaatga agttgatgga gaaagcggta aaaagtttat cgagttctta gtagatggaa
     2281 cacaggatgc agatcagccc ttgtccggtg gttatatttt aaccttgtgc catttccatc
     2341 atccttggac acatcgtggg tatctggtgg gcaagagttc tgctgatgaa attacagcga
     2401 tcttcacaac agccactact ttatggcagg aagaaaaaac tgctgaagca atttaccaat
     2461 tggggcgggc cttgcacttg ctccaggaca ttttcattcc tcatcacagc ggcattacag
     2521 gggtaaaagg acatggtgaa ttagaaaaat ggcttactga aaattggcta gcttatcaga
     2581 caacaagtgg tggctactat cagtgggagg aggattttta tcaccaaaat ggccatcagc
     2641 accatgtctc cagcactaat ccctatgact ggattgatta cggcagtcat ctctccatag
     2701 agtggtatga gcaatatttt gccgtaggca cttatgatga aaacactttc cgtaccgtag
     2761 ccccgcgcat cgtctcaaat gctctacgct tttctgcagg gtttattaat ttcttctttg
     2821 agcaggcagt tatagctgcg cgtgagcaat agttattttg ctttaaccac ctgctaaacg
     2881 cagcatcacc aacacttcat caccctccaa taaaatagtg tcccgggttg ccttttcttt
     2941 attcacgagg aaagttgcag cccggagtgc gtctatagtg gattgatcct gatgcttact
     3001 gagctgctcg agtaactgtc caatggttgt agactcagca ataattagcg aggcgggaat
     3061 aggttttagt acctccgcag ctaaaccaaa gtatttaact ttaatcttca ctttcttttc
     3121 acctcttttg taattaccag ttaagaaaag ctgccagcgc taaatacgac tggcagcttt
     3181 tttcctttat tcgtcgtaga ggtctttaat cacttcgtct aaacttccta gttcacgtaa
     3241 agaatcaagt gagggtttgc cagtttcaaa gtcccagtta acctgagcaa agaagttctt
     3301 gcccaacttt tcatggtcga tatcgacatc ggctacagga cctgatcttt gtggtggttt
     3361 accgacacaa cgagcaggaa taacaaagtc tttaggcgtt aaaccttccc ttaaattaaa
     3421 ggcgtggcgc aggttcaaaa tacgcaggcc agttttaaga ctatcttcgg ctgtaaatgg
     3481 cctgccggta acagcttcga gatatcttat ttcggcattt tgcagaccac cctgtgccat
     3541 gaaggtgcag aagcccacgc agttttgcac ttcctggttt gctgtcagca tcacgtctag
     3601 ctttccggta tcatcataat catatttatc ccctatcgga gcaccgaaca tttgaggaaa
     3661 ccctaaacca cctttgacgt gacgggccgg agtgggatca tattggtagg ttcgggcaaa
     3721 tcctggagct agtctggggt catgcattgg cagttcaatg cctaacactg tttgcaggaa
     3781 ttcggctcct ttaccccagg ttttggcggc agaagctgaa ccatgcgcta aaactgcacc
     3841 gcagccgtct tgatctgcaa ttttctgtgt tagtgcgaca atggcttcgc cgttacccca
     3901 ggttaaatca atgtcatcca attcttctct gcttaaaaca ccattattat agcattccat
     3961 tgcccaggtt actgtaccac cagttgagat ggtgtccagt ccatagcggt tacaaatctc
     4021 gttgcatttt aaaacagcat cagcttctga attcagcatg ttactaccaa aagcagctgc
     4081 agtttcgtat tcgggacgtt ctgtttgtgc taagggccaa cgtccagttt taactacata
     4141 ttcagcaccg caacccaagg gacagttagc acaattgtat tttcttactt tatgttgatc
     4201 aagcgcagca gcaccaagtt tttcggcagc gtcttcacca aaatcaacga caccaatgcc
     4261 gccccagttt ttaacgggag aatcaccatt taaggccgaa gcgggtgtgc ctgcaccggt
     4321 acccatctca ccaaaggctc gcgacccagg attatttttt agattaatca ccatttcctt
     4381 attcatcgct aatatttttt cttcctcaac catggcgata actccagtac cgcgcacagc
     4441 aatggcttta agtttcttag aacccatcac ggcaccacca ccaccacgcc caggggcacg
     4501 atgaccatca ttaatggggc aagcaagtaa agataccttt tcgcccgcag ggccaatggc
     4561 tactactcgt aactttggct cacctgtttc ttcgattaat gctgcccagg tctccttcgt
     4621 gtcttttccc cacagatgag cggcatccct aatttctact ttgccgtcat tgatatagag
     4681 gtaaacagga ttttccgaga ggccagaaag aaagacggca tcgaagccgg ctttctttaa
     4741 ttcgggaccg aagtagccgc cagaattggc atcgttccag ccacccgtta ccggcgactt
     4801 atgcactaaa gtgtaacgcc cgccaaaaaa tgcttttgtc gccgtcactg gaccggttac
     4861 aaaacctaaa atattctcag gacccaaagc atctgcacca ggtggcatca agttataaag
     4921 aattttagca ccaataccat aaccacctat ataatccttt gccaaatctt ctaggagttc
     4981 cttttcttcc caagtaccct tagccagatc cacaaataac attttgcctg cgtagccagg
     5041 taacatcact cttctcctcc ttcattttgc taaacctaga tcatgcatct tccattattt
     5101 actgatataa tacaagcgtt actaactatc ttcgagcttc ctcaccttga gtttggctgc
     5161 tgtttgaaaa atgtgaaaag catatttatt gcattagttc gacgccaggc ggccacatcc
     5221 ttgtagttaa gcaattatac ctgcctaatt aacaatttgt taatatcaag tcacacactg
     5281 gctatttgct ggctgccagg agcatgggca tagtcagcag cctgccgaca attttagcct
     5341 tgtaaaggaa gaaaaaagcc tgcacgctca tcttcagagt gcaagctttt tagttgatgt
     5401 gcagtatacg agtcttaatt agcaacttcg atgctagctg tggtgtctgg ttgctctaaa
     5461 cagaccagag cactaactaa acgatgctct ttaatttctg ttactttgat aatcaaacca
     5521 tactcttcta actctggtgt actaccatca tcaggaatgg tgcctaataa accgaaaact
     5581 aagccgccaa aagtctcata atcatcgtcc ggcagcttaa cacctaactg tttagaaagc
     5641 atttctaaag gtacagaacc ttgtattttc caggtctggg aatcaattct ttcaatgagg
     5701 ggtggctctt gtggaagtga actatcgtca tctagattcc ccaccagctc ttctaataaa
     5761 tcgttcatgg tgataatgcc actcatgccg ccataatcgt ccagtacaat agcaaaatgg
     5821 ttacggcttt ttttcatatt acgaaacaaa acatcggtgc gaactgtttc gggcacaaaa
     5881 taagcaggtt taacagcaag ctgcataatc ttatcgcgcg ttttgccttt aatgcgaaaa
     5941 taatcttttg catttaaaac acctacaata ttatcgatac tttcatcaca gagcggataa
     6001 atagagtgcc tgctctcgct aatcgtttcg tcccattgtt cagtgttttc atctaaccaa
     6061 agaagagaaa cttcagtacg atgtgtcata acttcgccag ctgatttatc gtcaaactca
     6121 aaaatgtttt gtatcatttc tttttcacta taattaatag cacctttttc actgcccgcg
     6181 tcgaccatca tgcgaatttc ttcttccgta attacgtcat cgatggccag gggatcaata
     6241 cctaataatc ttaaaagaaa attggtgcta acggttaata gccagacgac gggagcaaaa
     6301 atctgggctg taaaatagag aaagccagac atgcccagag caatctgttc tgacttacgc
     6361 atcgcgattc gtttcgggac taattctcct aaaacaagcg tgaaataaga taaaaacagg
     6421 gtgattagaa ttaccgcaat tgtgtctagc gtagcaatag gaatgttcac gccgacgttg
     6481 acgaggatag caactagttt atcggagaaa ttatcagcag caaaagcgct gcctagaaaa
     6541 ccagcaaaag tgataccaac ttgaatagta gaaagaaaac gagcaggctg atctgttaat
     6601 tgcaccaggc gaactgctct tttatcacct acggctgcca atttagccat tttattatca
     6661 ttcatggaga taacagcaat ttctgtaagc gcaaagagtg cattaaatag tattaagatt
     6721 ccctgtaaaa taaggggcca gaatatagag tctccatcgg gcaaaggaat aacctcctga
     6781 gattatattt ctatagatac gttactcgat atatcactaa tgtatacaag aaacacaccc
     6841 actagaatta ctccttcttt ctgcataaag aaatagttat aaataacgac acgaagccaa
     6901 gaacagataa tttaatctgt ctatgcagcc gtgccgtctt ttgctgtttc tgctgatcta
     6961 tatttataca tcgctcaggt ataggagaat catccaattt tgtctaacac tccttttcga
     7021 atatagtaaa taataccaca attttgcact ttcgtcaata catcgtaaac tagtcgggtc
     7081 ataaaagcca gtatctccag gtttccgttg ccgcttatca tgcccaaaag acctgtctgc
     7141 caggtggcag acaggtctgg agtgctatac tattctaatt cttggttaat aatttgatcg
     7201 gcatttttca tgattaaact aaaagcctct tgtgctgaaa agtattgcga taaagtggta
     7261 ttggctacag cctgatgctg taattgatat aatttttgaa actcctcatc tacaggcaag
     7321 ccatcagtga gcatttcttg aatatttact tgaatttgtt ccatttctgc cataatatcc
     7381 tgagctgctg gatcaagctt aatacgggct tgcgcctgac gtaggtcagc gaattctgtt
     7441 gtcgctttaa gttcttttgc tagttcttta gccgcattct cgatactaat tgttatcacc
     7501 tccagtaaga ctattctgct ctttttagta atctcctttt ccagaccaaa attttcctag
     7561 atttacttga tacataaatg catggcagca acaccgccgg aaaatgaatg tacgaagact
     7621 tgtgaaaaac cgttatcaat aaatagttga gctaattcgg ctttgtttag aaaattaaaa
     7681 gttgattgct gcagccagtt ataagcttcg tagttattgc tcacaatctt tccgattaag
     7741 ggcatcgcat acttaaaata aaaatgatac aattgtttat aaagaagcag actgggctgg
     7801 gatgtctcca gacaaacagc ggtgcctgct ggtttcacta cccgatacat ttctttgatt
     7861 acctgttggt agttttgtac attacgtagt ccaaagccaa ttgtaacata atcaaaacag
     7921 tcgttgctaa agggcagttg cagcgcatta tcacagataa gttccacgtt ttgtaggttt
     7981 gcttgggcaa ttttcgccct tccctgtacc agcatctgct gactaaaatc tagaccaaca
     8041 accttacctt ctgcacctac ggctttagct agctccactg tccagtcaca tgtaccacag
     8101 cacagatcga gaacacgctg accgggccgt agtgtcagtt gtttcattgt ttgcttgcgc
     8161 cagcgctggt gttgtcggaa actaatcaat gaatttatca aatcatagcg gtcggcaatc
     8221 tgttcaaaaa accgccaagt atttacttcc tgctttcttt gcatcaacat tcctgccttt
     8281 tcttgcttgc aaaatagtat tactgcaatg agacttttct atcccttcag tacgattggg
     8341 cgacatatat aatattgtca ttatgagagc ataaaatgta cattttgcga tgatagagca
     8401 ggatttagac cgaaatcggc gaaggcttgt gaaaaggata acctgctttt tgtattagca
     8461 gcagcgttgt tagattaagc gaaactgggc ctgatggcag cggaattatt aatcgtaaag
     8521 ggggtaaaga atatgcctaa acgtctgctt cttctcatct gcattttact atgcaccgta
     8581 tatttttcgg gtgtcatgca aagtaagcaa ctgccgtata tttctttgtg gccagatagt
     8641 ctacaagcag aaaagaatga tgtgacacca aaaactgaca cgatacgcat tgccattgta
     8701 agttccgctt ctgctagaaa gtcatttatc ttttatcaag attttgtcga ttattttgaa
     8761 caaaaagcag gtctacaagt ggaattgatc ttacgagaga actataacat tgccaatggt
     8821 ttagtctgtg aaggaagcgt agatttagct tttatctctt ccttttctta tgttttaagt
     8881 gaaagtaact gtaattattc cttattatta agaccaatag ctaaagatga cacaccttat
     8941 tgcagctgta ttatcgtgcg gggggacagc gacattcagt cactgaaaga gctgcgtgga
     9001 aaaactttta cctttacaga ccctttatcc actcttgggc gcctttatcc cttgtacctg
     9061 ctgacgacag cgggtgaatc tagtaataca ttttttgaaa atgtatacta tgcttctagt
     9121 acggattacg ccttacatag tttagatagc aggttagtgg aaggggcagc gatcaatagt
     9181 ttagtcttgc agtcactaaa tttgccgagc aaagaatttg cgagtagatt taaaattatt
     9241 catcaatctg aaccttttcc ccttaatccc ttagttgtta atactaacat gcaccctgac
     9301 cgtcaaaaaa tattaaagaa catcctcctg acgatgcatg aagataatcg tggcgctgct
     9361 atcttacacg atttaggtta tagtcacttt gtggcagcta aaccagcaga ttatgatcgt
     9421 atacgggaaa tggtaagtgt tgtaaaggac caactgccat gagcgaatct ttgctaaaaa
     9481 gaagtttgaa acgtctccgg gccgcttact ctccaggtgt tcgcagtaaa tttgtttttc
     9541 tctggatcgc tttagtgtta atcataggca tttgctttac ctggcaatct gaacgtatca
     9601 ccgagagtgt aattagaagt gatttacatg ctcgcggact atccattgcc cgtgatgtgg
     9661 cctcacgcag cgcagattcg atactcacac acaactatca cgaactacgg cagactatca
     9721 acgatacgct ggctaacaat aaggatatag tatatttatt cctacttgat cggcaaggta
     9781 cggtaattat taattcgcgt caagaccgac aaccatctac agcactgagg cagctgcaaa
     9841 cactacttcc agaagaagaa tttaaaacag caacttttcg ttcagaagag ggaattatcc
     9901 atgatcttgc tgtacccatc ttcggcggta atgccggcac tgtgcgcata ggcattaccg
     9961 aagcaaatct gcagaaagcc ttgactaaaa ccagaaacat actattcacg acgctgctga
    10021 tagctttggt cctgggtagc ttcattactg catattttac taataagtta ttagctcctt
    10081 tgcgccaact aacaacagcc actcacatta tcagagaagg tgatttcagt gcccgagtat
    10141 caatacaaac tcaagatgag attggacatc tagcaacagc atttaacatg atggcggaaa
    10201 aactaagcac ttacaaacta gaaaataaag aaaatcgtgc tgaattgaat cgcattcaaa
    10261 agttacgtgt tcagttagtg cagcatttaa ttacggcaca agaagaagaa cgcaagagaa
    10321 ttgctcggga gctgcatgac gaaacatccc agtcacttac agctctaaaa ttaggtttaa
    10381 aagcaatgga gcagacaacc tcaacagctg aaattcaaga tttaagtcat gaactgcgcc
    10441 agatgttagg ggcaacactt aatgaaataa ctgctatttc acgcaactta cgacccagtg
    10501 tgcttgatga tatgggactt gtggcagctt tggcacgttt tttaggggat tcctcagtcc
    10561 gtttaggcaa aagaattgcc tttaaacatg agggattagt aggaaaacgt tttcctgttt
    10621 acgtggaaac agcaatttat cgcgtggttc aggaagccgt cacaaatagt cttaagtatg
    10681 cacacgctga aaatatcttc gtcagtgtga cctacattaa taacctactc aaagctatcg
    10741 tggacgataa cggggtgggc tttgatccaa caaccgtttt tgatggggat attccccaag
    10801 agggatttgg tttattcggt atgcaagaac gggcgactct gattgacggt accttgcaaa
    10861 ttgactcagc aataggacga ggtactacca tcactgttac tgtggcaaat gtacaagaag
    10921 ccggggcata agctccggct tcttgtttga aacaagtctg tttcacgctc tgaacaacga
    10981 gaaggaaaag cgtgcttaag gggaagcaaa agtacgcttt tcttgaattg gcttttgtga
    11041 tgaccacttt catcattagt gaagtcctct tcttctgctt ctatctttac actctccagc
    11101 agccaagatt tccttatttg gcttcgtttt ctgttggcag tactttttct gtttcgcgtt
    11161 tgttttccca taataacaag ccaaccagcg ccacaacgaa gattcgtaat ggtcgaaaaa
    11221 tagcattcca gatataggcc gaaataggta ttttggggtt agcaggcaaa ccatattctt
    11281 caggatcatc ttttaagaca tacataactc ctgtgccatt taactcttcc agaccataaa
    11341 tttgtgcctt gctagaaccc tggacgacca aatcgttaac acgacgatga gccaaatcta
    11401 ccatttctgc acgtgtacca aattgtatag caccagtggg gcaggcctta gcacaggccg
    11461 gaattaagcc tatagcctga cggtcgctgc ataaagtgca tttttgggag atattttcct
    11521 tttgattaaa ggacattacc ttaaaggtac agctagccac acaatagtta caaccaatgc
    11581 attttttagg gtctgttact acagcaccgg tttcagtatg actaatggca ccagctggac
    11641 atactttttc acacgcagca tccgtacagt gcatacaacc tatttttgca aaataccaac
    11701 tcacatcacc tgttgactca tcttcatgct cacggaaaga tacacgagtc caagagttag
    11761 cggtaaaacg taccgggttt tcataaaaac cggtaaagct agtttcttca gccggtagtc
    11821 tattccattg cttacaggct acttgacagg cacggcagcc aatacattta gatgtatcga
    11881 taagtagcga atttaacatt ttatcccacc ttccttatgt tgcagagaaa ggctttgtat
    11941 tcaggtatat tagtattagc atctccgatg gaaggtacta tatcgttgac aactgcgcct
    12001 ttatttaagc ccataaagcc ccagtggaag ggcatgccaa ccatttcccg cagttctccg
    12061 tcaaccagca gaggtttcat gcgtggtgtg atacaagcga gagcagtgat gtgttgctta
    12121 cggtaactgc tgatctccac ctgatctcca tttttgatgc ccagcttctc tgccaatgtt
    12181 ggactgattt caatgaagag tgctggcatt agttcattta accacggtgt attgcgcgtc
    12241 ataatccccg actgccagtg ttcactcaca cggtaggtgg agatcacata agggaaattt
    12301 ttatccccag cagctgccaa gtgatcttca taccaaattt ttgtgactgg gttaaattgc
    12361 accgtcgtag atagtatgtt gcggatggga ctttctgccg gctcgtaatg ctctgggaaa
    12421 ggcccgtcag ccaaaccgct ggcaaagaga cgagcttgca gttcaggcag cataatataa
    12481 ggcgcttgcg ctgtcgtttc tggtggtaca ttagcattaa agtcaggcac atcgttacgc
    12541 tgccaagtta gaccatccca ccacattagc ggagtatccg gattccaagc atttccttgc
    12601 ggatctgctg aacaacgatt atagatgatc cgcctgttta gcggccaggc aaatgaccac
    12661 ttagcgtttg taccaatacc ggaatcttct ttgatccggc ttttacaggc tggatgggta
    12721 gcgttattgt aatatccact gtaaatccaa cagccgcagg cggtactgcc gtcatcagcc
    12781 agtttggtga agtcttctaa taataagcct gtcttaagat cataaccgtt aatttccatg
    12841 gccacagcat tagcatcagc ctctgcaccg taatcccact tcatttttag aataggctcg
    12901 gcaaaaggac cggcattctt ttcatattcc tttttgactg ctttgaaaag ctgatcgaca
    12961 atccagatat cagtatgggc atcccctaga ggttcttgtg cttgatagcg ccactgaatc
    13021 cagcggccac tattcgctat tgtcccttct ctttcaaaag agactgaact aggcaggaga
    13081 aaaacctctg tgtctatctc agttggatca accccgggtt ctttccaaaa agcagcagtt
    13141 tcattttcaa agatatcaat ggaaaccagc caatctaaat tttgggagta atttcttttt
    13201 gtagaggcag aaggcccggt tacagcggga ttgtctgccc aacagatcat gcccttaatt
    13261 tcaccttcac ccatatattt gtacatgcca atatgggaac gatctttagt atctaacttg
    13321 gggacataat cataacaaaa gtcattatcc tgtgtagcat aatcaccata ccaggcttta
    13381 agcatgctga ttaaaaattt tggtttgttt gtccagtaac caccagcagg ggtttccttt
    13441 tccagataag cagccaaatc ctcatgcagt gctgctttgg gcatgtttag gtagccgggt
    13501 ataagatgat aaagcatcgc catatcagtg gaaccctgca cattagcctg accacgctgc
    13561 gcgttaacgc cgcccccggg gatacccatg ttccctaaaa gcagctgcag acaagcaatc
    13621 gctctcacgt tttgtgagcc atgcgtaaat tgtgttacgc ccatggcata catgatgttt
    13681 cctgccttac cgggctgacc agtgctggca aataattcag ccactgcttg taacttgtca
    13741 accgggcagc cggtaatccg gcttacagtt ttaaaatcgt agcgttcata atgttttttc
    13801 atcagttgga gaacagaatg ttgatcagtc gcagtaggat cttttatcac tttaccctct
    13861 gcatcaactt gataactcca agagtcttta tcataattta cagcacgatc ttcagcatca
    13921 tgtgctcctg agaataaacc atcgttaaaa gaaaactctg gattaatcag ataagtagcg
    13981 ttggtgtaat taaggacata ttcttcatgg tatagcttat tgctgagcac ataattgatc
    14041 aacccgccaa gaaaagcgat gtttgtgcca gggcgcagag gggcgtacaa atctgcggta
    14101 gcagcagagc gggtaaaacg gggatctacc acaattaatt ttgcaccttt ttctgcacga
    14161 gcccgctcaa cccatttcat ggaaatagga tggttttccg ctggattaca gccaaaggct
    14221 aaaaagacat cagaattctt ataatcaatc caatggttcg tcattgcacc gcgaccaaat
    14281 gagttgccca gaccggcaac tgtggaggag tgtcagagac gggcacagtg gtcgatattg
    14341 attatcccca aggcacggat taacttatga tatagatagt tttcttcatt agtacagttt
    14401 gaacttccta aatgcgcaat cgctcttgtg cgatttacgg taacaccgtg ctcatcaact
    14461 gtttcgaagg tggaatcgcg tgtttgctta atgcgttggg caacttgtgt taaagcccag
    14521 tcccagtcta cttctttcca ctcggtagca cggggcgcac ggtagagaac ctttttcata
    14581 cgttgtggat gttcaatggc ttgaccttgt tgatcataaa cataagcaag gttaaaaatt
    14641 gaactgcctt tactgcaaag agaaccttcg ttaataggat gatcagggtc accttctgca
    14701 cccacgactt tgtcattttg cacataacaa ataatcccac agcctacaga acaataagga
    14761 catatggttg ttacttcttt tgcatattgc acgggcactg gtgcagcttc agtcgtgtta
    14821 gagaaaccaa agctggctaa agtagctgag ccaacagctg cgcccgttag tttgagaaaa
    14881 ctacgacgag atactttcat tcttgctcac ctcctgctag ttttttgcag cagcttttac
    14941 gctcgccggc acagcttgcg ggacacgctg tactactggt tcaccaaatt tctttcttct
    15001 ggttaaacga tcaattagcg gcacaaaagc gttcatgata aggatggcga aggcaacccc
    15061 ctcggttggc gccaataaaa cacggaaaac aacaacaatg atcccaatac agactccgaa
    15121 gattatcttg cccttcttgg taatggggct ggtaacccag tctgtagcca taaagaaagc
    15181 accaagtagt aaacccccac ttagcagatg ataaattggg tgttcaccta agacggcagt
    15241 gccaacaaaa acagtggcca gcatggaaac aggtatatgc caagtgatgt gctttttata
    15301 taacagataa gcaccaccta taagtaaagc taacgctgaa gattcggcaa tacctccccc
    15361 ctgggtagca atgaaaaatg tccccatatt aggtaaaggc aaccccattt gcagcatagt
    15421 cagtggcgtg gcttgtgtta ccgtatcaat accgggaaaa ctaacccgcc aatagaaaaa
    15481 ttcattattc agataaacta accaaggggc aaacagaatt acagatactc tgccaaatag
    15541 tgctgggtta aaacgattcc agcctaaacc gcctaaaaat tccttggcta aacccactgc
    15601 aatgaaagtg gccgcggcgg ctaaccacca tggtgtggcc gccgaaaaac tcaaggcaac
    15661 cagtactccg gttaaaatag cactgccatc ttgaagttgt ggttctttac gcatcgcacg
    15721 gcggaatatc acttccgtaa agatggccgt aactacacag actaagatta aaaatggcac
    15781 atttaattta aagaaataac atgccaccag cgttacaggc attagtgcaa tggttacatc
    15841 ccgcatcgcc gtagacacct gatcaccagc atgtagatgt ggtgctgaag aaacctgcaa
    15901 attcaaatct tttcccaagt tgacccctcc cttcttcctc tttacgccct tttcacccgg
    15961 cggacaaact caactattgg tctctgagat gggcagacaa aagcacaaat tccgcactca
    16021 aaacaatcaa gagctcccca ctctttggct aaggcatagc ggcctaattc cccatacatc
    16081 ccaatgtagt tgggataaag acccatggga caatgatcca cacatttacc gcagcgcaca
    16141 caagaataat attcctgcgt tttaaccgcc agttcttctg ttaaggccag cactccgcca
    16201 cagcctttta caattggagc agccaaagtt tcttgtgcaa aacctgtcat gggaccgccc
    16261 agcacaactt tagtgaccct atctgttagt ccgcccgtac aagcaagtaa gtgggaaaga
    16321 ggtgtaccga ttacgacccg gtaattgcct ggttgtacgg cagccgctcc cgttaaggta
    16381 acaatccgtt ctgttaaagg gcgctggaat tttaccgctt cccaaacagc tttggctgtg
    16441 gcgacattct gtaccaccac attcacttcc gctggcagct tccccagggg aaccactcgc
    16501 cctgttgtcg cataaataag ttgtttttct gctccttgcg ggtactttac ttccaaagga
    16561 atcacttcca tgtcggcttc gctcgccaac ttggccttga caacttcaat ggcgtccatt
    16621 ttattttctt caatgccgaa aattacttta gcgccgttta acacctggct gaggatacgc
    16681 gctcctgcta tcatttcatc agcttcttct accattaaac ggtgatcaca agttaaaaat
    16741 ggttcacatt cacagccatt gaggattaaa taatctatgc tagtaggagg gttgaccttc
    16801 acatgagtag gaaaacctgc cccccccata cccacgatgc cggcctctct aatccggcgc
    16861 ctgatctgct ccggggacaa ttgcgagaca tcttcttcgg tataaacaca ggcatcttgt
    16921 tcaggatcca catcaattac gacacaatct tccagttgtc ccgacaaagt tactctgctg
    16981 ccaatttctc gtactgtccc acttaaccct gtatgtacag gtgcggagat atttgcatca
    17041 ctatcgccga ttttttgtcc ggccttcact ttttccccca cagaaaccag ccgcctattt
    17101 ggtgcaccaa tatgctgttt tagaggaata attaattctg taggttgtgg caggtcttgg
    17161 atgggcagat tttcggttag tttcttgtaa tgaggaactt cctttcctct ttcaaatgtt
    17221 tttgccacta gtttcacctc ccttagttgt atcagttaaa agttgtttta tcaccagatt
    17281 caccatatcc ctcatcagca gccagcacat caagaaagtg cgtcattaaa ttttccaaat
    17341 cgggaataat gtctcttgct aattctttta agacaactgt cttaagatag ttatgacact
    17401 gactacagac atagacacgc cgatggactt gatctggaag atagaaaaaa ctaagtttac
    17461 tatggtcggt attaccgcag gacatacaac gtatacggct tacctcccat tgtgtatgac
    17521 aaagtccaca ctccaaaact ctgctgccat cttctgccct taacatggct agcattggtt
    17581 tctcgccaca aactggacaa acaccttcgt tccaattact agcatcaata gcggcctgca
    17641 tctgctgtgc gaagtacaca aaatacattt gcagtgccga gcgaatcact tgagctgtaa
    17701 ccgccagctg ttgctcggcc aactcagcag tatcatgttt agtgaaaaga aatttctgca
    17761 ccttagctaa agataaattg ctcggtctac tgtaaaactc cttgatatta tcagccgtga
    17821 aagtgctttg catgagattg cacatcacgt tcagctgttg ttcattcttt accaggagtt
    17881 cactacaagc atctaataaa atggtttgtg ccagctctaa tgtcggttgc acaggtatcg
    17941 gcagtgcgag ttcagctaag agtggcttgc cctgcataac ctctctgttc gcagtctggg
    18001 gtggtgagta agtggttaaa gaattggtaa agcccctttg gtgggcatat agcttttcat
    18061 aaaaatgcaa tagtgatttc atgttttggt gctcttgtaa ataaaacttc acttcttgct
    18121 ttactgcttc atctgtcgga gggtaattca ccacagcaaa agctcctttc ggctattgtg
    18181 atttttttca cctgactatt aatcaattat gacactattg gcagcaggaa taaatcaggt
    18241 aaatcagcag atttacaaga gatagatcag ggtttttccc cggactgtca gtattttacc
    18301 tacattcata aaaatttgct cattctggtg ccagattttt ttttccagta cccactcctg
    18361 cgacgtttag atgatagcca gaaaaaaaat aaaagagccg caaaggctca tggagtatgt
    18421 tgaaactgtt ttagttctaa ttttaaatct tgttttaagt gaatgatctt tttacaatat
    18481 tcgtagacaa cccagcccgc aggtgtgggg gtaacccctt tgttgctgcg tttcaataaa
    18541 cgctgttgac aatattgttc caaatcacgt acctgcatgc tgacagcagg ctgtgaaata
    18601 tggcagtagc gagcggcctg ggagatactg ccagtttgaa ttactgtgca gaaagcttct
    18661 atctgtgcca ttttcaagtg atactcattc ctttcttgaa gttcggggtg catacacatc
    18721 tctccttttt tattagctaa ctgctaacgt taatataatt tggctgacag aatttcttgt
    18781 aaggtaatta aggtaattca ctagggtcta gaggaaaagt caagactttt ctttatgttt
    18841 ccaaatcaag caagttgtgt tccatcgcat atgccacaaa ttctgaacgc tgtacaatat
    18901 ctagcttttc tttaattctc gctttatgac tttccacagt tttcacacta acatggattt
    18961 gttcagcgat ctgtttatct gtgtagcctc gggctactaa gctaagagtc tctttttgcc
    19021 gtttgctaag gatttctttt tctttacttt gttgtttttt gtcaacaggc tgcagataac
    19081 gctgccacaa tctcttggtc aaagaagaat ctacaaccat ttcgccgcgc acaattgcac
    19141 gtatagccag aatcagctca tcatctgctg ctttttttag caaataacca tctgcacctt
    19201 ctgtaagtac ttgttggatg taatcatctt cttcgtggat agttaaaact agaattttaa
    19261 tttgaggaca aattttctta atttctttga taaattctat gccactgcga tcaggtagag
    19321 ttaaatcgag gataattaaa tcaggcataa gctcctggac tatagttatc ccctctccga
    19381 ctgaacctgc ttcaccaatc acttccatac tctcttgcgt tctgattatt aaatgtaagc
    19441 ctttacgcac aatgggatga tcatcaatta acataatttt aattgtgtca ttacacataa
    19501 ttttcacccc ttttcaccag acatagaacc atgttaattt tttcacaaag tttagctata
    19561 cattctagct gctaccgcca ttatcctgct aatattgtaa aatattttaa taattatgta
    19621 atctttcttt ctgtcgcata tttatgtagt tttgggaaaa actttgtttg tccaagattt
    19681 gaaaactatg tagattttgt gataagatta agaaggtttt ttctggggtt tgcagaatat
    19741 atttcattg
//
LOCUS       DASRMS010000074         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_832183, whole genome shotgun sequence.
ACCESSION   DASRMS010000074 DASRMS010000000
VERSION     DASRMS010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_832183"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1032)
                     /locus_tag="VFC74_03285"
     CDS             complement(<1..>1032)
                     /locus_tag="VFC74_03285"
                     /inference="COORDINATES: protein motif:HMM:NF039179.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24395.1"
                     /translation="DAAGIGSGYGKHEGGIVIIRGGETEASCWLNSSKNPNSSGAAIG
                     GGCYGDGGIITISGGKVTAKSFSGAAIGGGREGAGGTITITGGDIAAECVSTGSAIGG
                     GDSGNSGNITISGGDVKAESRYEAAIGAGKNGYCRTINITGGKTRAISAITGNGIGGS
                     KTGIGGTVSISGGIVIVGAGTYNKHEQIRDIGVGEDNKLDLLEVSGDAVLLLKNNRYN
                     DPIATTSHVFKTASSSSDYEKYNLPLSPADESSLDQWCSSGHKLGIWSPAAPGKKIVT
                     AVSIASKPDKFDYFTDEELDLTGGQLFVKTIHNEKNFKVSMTDPRVSFTGFNSNPETY
                     GPQTITVKFA"
BASE COUNT          259 a          263 c          203 g          307 t
ORIGIN      
        1 gcaaacttta ctgtgatggt ctgagggcca taggtctcag gattagagtt aaagccagta
       61 aaagaaactc ttggatccgt cattgaaact ttgaagtttt tctcgttatg aattgtttta
      121 acaaacagtt ggccaccggt cagatctaat tcttcgtcgg taaaataatc aaatttgtct
      181 ggtttagaag caatgctgac agcagtaacg atcttttttc ccggcgctgc gggagaccat
      241 ataccaagct tatgaccact actacaccat tgatctaagc tgctttcgtc agcaggagaa
      301 agtggtaaat tatatttttc atagtcagag gatgaagaag cagttttaaa aacatgactt
      361 gttgtagcga tgggatcgtt gtatctattg ttttttaaca ataaaacggc gtcaccggat
      421 acttctaata aatcaagctt attgtcctct cctacaccaa tatctctaat ttgctcatgt
      481 ttattatagg tgccggcacc aacgataaca attccgcctg agatggaaac tgttccacct
      541 attcccgttt tactgccacc aataccatta cctgtgatag cactgatggc cctagttttg
      601 ccgccggtta tatttattgt cctgcagtat ccgttttttc ctgcaccgat agctgcttca
      661 taccgtgatt cagcttttac atcgccgccg ctaatcgtaa tattcccact attaccactg
      721 tctccaccac caatagcgga cccagtggac acacattctg ccgcaatatc gccacctgtg
      781 attgtgattg taccgccggc gccttctctt ccgccaccaa tagcggctcc ggagaagctt
      841 ttagcggtta ccttgccgcc gctaatggta atgatgccgc catctccata acagccgcca
      901 ccgattgcag caccggagct attaggattt ttgcttgaat tgagccaaca gctggcttct
      961 gtttctccgc ccctaatgat gacgatgcca ccttcatgtt tgccataacc tgacccgata
     1021 ccggctgcat cc
//
LOCUS       DASRMS010000075         2734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_152364, whole genome shotgun sequence.
ACCESSION   DASRMS010000075 DASRMS010000000
VERSION     DASRMS010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2734
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152364"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..130
                     /locus_tag="VFC74_03290"
     CDS             <1..130
                     /locus_tag="VFC74_03290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004603178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="iron dependent repressor, metal binding and
                     dimerization domain protein"
                     /protein_id="HZK24396.1"
                     /translation="TEFFVRLGVDEDTAREDACKVEHDISEETFEAICRHVESREQ"
     gene            complement(272..1621)
                     /locus_tag="VFC74_03295"
     CDS             complement(272..1621)
                     /locus_tag="VFC74_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK24397.1"
                     /translation="MRLLVIGAVAAGTSAAAKARRNNEKAEIVLYEKGSHISYAGCDT
                     PYFIGGVIKDVKSLTPRSVDFFKKKYAVDVKIRHEVKKIDLAAKTLTVKNQDQDETFV
                     DHYDKLIIATGAKSVILPIPGIKQDHVFSLRNIKDALAIDSFIADKKPQRAAIIGSGS
                     IGMELCENLKLRGLDISMIEQAHHIHPALDPDMSAHLEKQLLSNNVRIFKNTRAAKIE
                     ANHILTSDDEKIPCDMVIVAAGVRPEISLAVEAGIKLGETGAIAVDKYLQTSDPDVYA
                     CGDCIETFNLITKKASYFPLGSTANKTGRIAGDVVTGGNLSFRGVLGTGIFKVFDLSV
                     ARTGLSERQAREAGYDIEAAHIIKPDKTAIAGGQELIIKAIADRKTKKLLGVQILGTE
                     GVDKRIDVLVTAMTLGAKVNDLFHLDLAYSPMHSIAKDPVMYVGMVLDNAIAASKTQ"
     gene            1901..2635
                     /locus_tag="VFC74_03300"
     CDS             1901..2635
                     /locus_tag="VFC74_03300"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008678880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HZK24398.1"
                     /translation="MANRLEGKVAVITGGASGIGAAAARRFVEEGAKVVISDVNVKDG
                     QALADAYKESMIFVEHDVAKAKDWEKVRDAAVENFGTIDVLINNAGISVAKSFFTMSE
                     EEYRRIVDINQVSVFLGIKIVGEVLRAKGAGSIINTSSINGLIAGAVGYTDTKFAVRG
                     MTKAASAELAPQGVRVNSIHPGIIETPMVTEGDAVEQIREYAKHVPMRRMAQPEEVAN
                     LMLYLASDESSYSTGSEFVVDGGLTQ"
BASE COUNT          761 a          569 c          622 g          782 t
ORIGIN      
        1 gacggagttt tttgtacgtc ttggcgtaga tgaggacacc gcccgggagg atgcctgcaa
       61 ggtggaacat gacatcagtg aagagacctt tgaggctatt tgcagacacg tagagagtcg
      121 agaacaatag taattaggga actccttcca aaactatagt tttggaagga cacactaggc
      181 aatatgccgc agatggcatt acagtcaaac tgcaaggctc agccgaatct ttgaaagcaa
      241 ctctgcaata attctatctg caattgtcgg ttcactgagt tttacttgcg gcgatagcat
      301 tatccagtac catgcctaca tacatgacag gatctttggc gattgaatgc ataggagaat
      361 atgccaggtc aagatgaaat aaatcattga cttttgctcc cagggtcatg gcggtaacga
      421 gcacatcaat gcgtttatca acgccttctg ttccaagtat ttgcacccca agaagttttt
      481 ttgtcttgcg gtcagcaata gctttgataa tcaattcttg tccgccggcg atagctgttt
      541 tatcgggctt tataatatgt gccgcctcaa tatcgtatcc tgcttctctt gcctgccgct
      601 cactcaagcc ggttcttgcc acactgagat cgaaaacttt aaaaattccg gttcctaaaa
      661 ctcctctaaa acttaagttc ccaccggtaa ctacgtcacc ggcgatgcgg ccggttttat
      721 tagctgtaga acccagcggg aagtaggatg cctttttcgt gatcaaatta aaggtctcga
      781 tacaatcgcc gcaggcgtaa acatcaggat cgctggtctg cagatacttg tcgacagcga
      841 tggcgccagt ttcaccaagc ttaatgcctg cctcgactgc cagggagatt tccggcctga
      901 cacctgcggc aacgataacc atatcgcagg gtattttctc atcgtcagaa gtcaaaatat
      961 gattcgcttc aatttttgcg gcgcgagtgt ttttgaatat tctgacatta ttggacaata
     1021 attgtttttc gagatgagcg gacatgtccg gatccagagc aggatgaatg tgatgcgcct
     1081 gctcaatcat gctgatatcg agtccgcgca gcttcagatt ttcgcacagc tccatcccaa
     1141 tgctgccact gccgataatt gctgccctct gaggcttttt atcagcaatg aatgaatcga
     1201 tggccagtgc atctttgata ttcctaaggc tgaaaacatg atcttgttta atgcccggga
     1261 ttggcaaaat aacagatttt gctccggtgg ctatgataag tttgtcatag tgatcaacaa
     1321 aagtttcatc ttgatcctga ttcttaacgg taagcgtctt cgccgccagg tcaatttttt
     1381 tcacttcgtg acgaattttg acatcgacag catatttttt cttgaagaaa tccacactgc
     1441 gcggagtcaa tgacttgacg tctttaatta caccgccgat gaaataggga gtatcacaac
     1501 ctgcataaga tatatgtgaa cctttttcgt agagtacaat ctcggccttc tcattattgc
     1561 ggcgcgcctt agcagctgct gatgtgcctg ccgctactgc cccaatgact aaaagtctca
     1621 tcttaagcct cctgcaattt tcgaattaaa tttagtttat catgtaagtt ttctgattga
     1681 gcgcaattaa gtaacaagta tcacggtttt agattgaaag ttttgcaaat ttagtgtcta
     1741 atagcaaagt gctccgatga agtctatgag ccgagccaat gtggataccg gctaattcca
     1801 gccaataatc ctagtatatg cgttatttag tgtgatccaa ttacagatcc actttaaata
     1861 tttaaagatg ttcaaataaa aaacaagtga ggtaaaaatt atggcaaatc gtttagaagg
     1921 caaagtcgcg gtcattacgg gaggagcgag tggtatcggc gcagcagcgg ctcgtcgttt
     1981 cgttgaagaa ggcgcaaaag tagtcattag tgacgtaaat gtaaaagatg gtcaggcatt
     2041 ggctgatgct tacaaagaat caatgatttt tgtagaacat gatgtcgcaa aggccaaaga
     2101 ctgggaaaag gttcgcgatg cagctgttga aaacttcggt actatagatg tcttgatcaa
     2161 taatgcaggt atctcagttg caaaatcttt ctttaccatg tcagaagaag aataccgcag
     2221 aattgttgat attaaccaag tttctgtttt cttgggtatt aaaatagtgg gcgaagtttt
     2281 gagagccaaa ggtgccggtt ctattatcaa tacgtcttca atcaatggtt taattgccgg
     2341 ggctgttggt tacacggata caaaatttgc ggtgcgtggg atgaccaagg cagcttctgc
     2401 tgaattagct ccccaaggtg tgcgtgtcaa ctccatccat cccggaatca ttgaaacgcc
     2461 aatggttact gaaggtgatg cagtggaaca gataagagag tatgcgaaac atgtccctat
     2521 gcgtcgtatg gcccaaccgg aagaagtagc caacttgatg ctgtacttag ccagtgatga
     2581 atcaagttat tcaacgggtt cagaatttgt tgttgacggc ggtttgacac aataattatt
     2641 tttgactgat taagtcaaaa aatccggacc gaactttata agatacatcc tcgtatcgca
     2701 gttgtaaaaa atgtgcaaat gcgtcagagg atgt
//
LOCUS       DASRMS010000076         1084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_354074, whole genome shotgun sequence.
ACCESSION   DASRMS010000076 DASRMS010000000
VERSION     DASRMS010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1084
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_354074"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            118..>1084
                     /locus_tag="VFC74_03305"
     CDS             118..>1084
                     /locus_tag="VFC74_03305"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK24399.1"
                     /translation="MGIKTTETKSKLALLIIVTMLLGVLSPAMAAGTDRAVGFTDIEN
                     HWAKDTIIKWTEEGLIKGYEDGKFKPNNNISRAEFMAVINRALGFNEEATIEFSDVGP
                     DDWFYADVAKAVKAGYIEGYEDGSVKPAQDISRQEAAVALSRALGIAGREQGSQFTDG
                     ADIADWSLPYINAFTERGYIEGYSDGSFQAQRSISRAEAVTMLDKARADIEEDIGGEE
                     SEPEEDDEDDEAPDSGGGSSSSGNGGSSSSVEVSAISVSPESITLAGGSTSTITATVS
                     PTDATDKTVIWTSSDEDIVRVDETGNVTAVGVGTATITATADGKSA"
BASE COUNT          327 a          186 c          310 g          261 t
ORIGIN      
        1 gttttaaatc aagcttaagt tctaatcagc aggtttttta aagggctgct gttcttgtaa
       61 aagtttctgg gttaggcagc attttaattt tcaaaaataa aaagcggagg tattggaatg
      121 ggaatcaaaa ctactgaaac aaaatccaag ttggctttat tgattatcgt gaccatgttg
      181 ctgggcgtcc tttccccggc catggccgcc ggtaccgata gggcggttgg ttttacagat
      241 atagaaaacc actgggctaa ggatacgata attaaatgga cagaagaagg cctaattaaa
      301 ggctacgaag atggcaagtt taaaccgaat aataacataa gcagggcaga gttcatggct
      361 gtgattaaca gagccttggg ctttaatgaa gaggctacca ttgaatttag tgatgtgggg
      421 cctgatgatt ggttttatgc agatgtggca aaagctgtaa aggcaggtta catagaaggc
      481 tatgaggatg gtagtgttaa accggctcag gatatcagcc gacaggaggc tgctgtggcc
      541 ctaagcaggg ctttaggtat agccggccgg gagcagggta gtcaatttac agatggagca
      601 gatatagcgg attggagttt gccctatatt aatgccttta ccgaaagggg ctatatagag
      661 ggctattcgg atggtagctt tcaggctcag cgttccatta gccgggccga agcagtgacc
      721 atgctggata aagccagggc ggacattgag gaagatatcg gcggggaaga aagtgagcct
      781 gaagaagatg atgaagacga cgaagcccct gacagcggcg gaggtagtag cagtagcggt
      841 aacggcggca gtagcagtag tgtagaggta agtgctatta gtgttagtcc tgaatctatt
      901 acattagcgg gtggatcaac atcgacaatt acagcaactg tatctccaac agatgcaaca
      961 gacaagacgg taatatggac ctcaagtgat gaagatatag ttcgtgtcga tgaaactgga
     1021 aatgtaacag cggttggcgt aggaacagca acgattaccg caacagctga tggtaaatca
     1081 gcaa
//
LOCUS       DASRMS010000077         1329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_287647, whole genome shotgun sequence.
ACCESSION   DASRMS010000077 DASRMS010000000
VERSION     DASRMS010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1329
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287647"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1270)
                     /locus_tag="VFC74_03310"
     CDS             complement(<1..1270)
                     /locus_tag="VFC74_03310"
                     /inference="COORDINATES: protein motif:HMM:NF013079.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HZK24400.1"
                     /translation="MNLFSKKEIDQAQNLVIVFSQLKQLDQTAKEIKEKAESDNELLA
                     KTEGMQFYRQGLAGGGDLYLLKVSPDVKSNRKLQKKFHALACSFSKNSSSDLQFSFLA
                     DLSLEQKLQCLKAFLLGLPAYKAHTSSGFKPEKDMDPKEIKKNLAVDEAKDDSPKGLA
                     AQLVARAENLKLADDKSEQSLKNIALIEEDDLLEKKLTELQALASSIYLGRELVNERA
                     NLMTPTELAAVASLIGEQSEIEVEIFEPAAIEKLGMKAFLEVAKGSDEEARLIVMRYR
                     GKPDSDKTLALVGKGVMYDTGGYALKTSKGMFDMFSDMAGSAAVISAIRALAETKAEV
                     NVTAIVAACENMVSGHAFRNGDIVESMSGKSIEVVNTDAEGRLTLADAVTYAWQKENA
                     DYIVDIATLTGAVVVALGDKMTGGLTDDQEL"
BASE COUNT          340 a          316 c          255 g          418 t
ORIGIN      
        1 aaagctcttg atcatcagtt aagccaccgg tcattttatc acccaaggcg acaacgacag
       61 ctccagtcag cgtggcaata tccacaatat agtcagcatt ttctttttgc caggcatagg
      121 taacagcgtc agccagagtc aggcggcctt cagcgtctgt gtttactact tcaatactct
      181 tgcctgacat ggactcgacg atatctccat tgcggaaggc atgtccggaa accatatttt
      241 cgcaggctgc aacaatggca gtgacattta cttctgcttt agtttctgct agtgcgcgaa
      301 tggcagagat cacagctgct gaaccagcca tatcactaaa catatcaaac attcccttgc
      361 tagttttcag ggcatagcca ccggtatcat acatgacgcc tttgcccacc aaggctaagg
      421 ttttatcgct gtcaggcttg ccacggtaac gcatgacaat caggcgtgct tcttcatctg
      481 aacccttggc aacttctaag aaagctttca ttcccagttt ttcaatggct gcaggctcaa
      541 agatttccac ctcaatctca ctttgctcac cgattaggct tgcaacagcc gccagttctg
      601 tgggtgtcat caggttggca cgttcattga ctagctcgcg tcccagatag atagatgagg
      661 ccagagcttg caactcggtc agcttttttt caagcaagtc atcttcctca attaaagcta
      721 tatttttcaa agactgctca gacttgtcat cagctaattt aagattttca gcccttgcca
      781 ccagctgtgc agctaaacct tttggactat catcttttgc ttcatcaact gctaaattct
      841 tttttatttc tttaggatcc atatcctttt ctggtttgaa gccgctagaa gtatgtgctt
      901 tataggcagg caggcccagt agaaaagctt tgagacactg tagtttttgt tcaaggctca
      961 aatctgccag aaatgagaac tgtaaatccg aacttgagtt tttgctaaag ctgcaggcta
     1021 aggcgtggaa ctttttctgt agcttgcgat tagatttgac atctgggctt actttcagca
     1081 aataaaggtc gccgcctcct gccaaacctt gccgataaaa ctgcatgcct tcagttttag
     1141 ccaagagctc attatcagat tctgcttttt ccttaatctc tttagctgtc tggtccagct
     1201 gcttgagctg ggaaaaaaca attactaaat tttgagcttg atcaatttct ttttttgaaa
     1261 ataaattcat atatcctcca ttaaaatttc ttaaatattt tatttttaaa agcttgtttt
     1321 caagttagc
//
LOCUS       DASRMS010000078         1133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_287702, whole genome shotgun sequence.
ACCESSION   DASRMS010000078 DASRMS010000000
VERSION     DASRMS010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1133
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287702"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..446)
                     /locus_tag="VFC74_03315"
     CDS             complement(186..446)
                     /locus_tag="VFC74_03315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24401.1"
                     /translation="MGSNAERLKRYRERMAANGFKRLSFYAAPELVELIAKERQPYEC
                     GGRTLERLLLGEAKPRPEYWTAAERAQKAARRAASGRQITKV"
     gene            complement(777..>1133)
                     /locus_tag="VFC74_03320"
     CDS             complement(777..>1133)
                     /locus_tag="VFC74_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24402.1"
                     /translation="ALKEAVATDPELEVLTEALEGIEKLMGDGWFETRELFKKLNNPI
                     YGFSDMRADGGDGGDLKELLQDLTGANDLTSRGLGRVLGYRVDRIAGGRKLVKRTGGK
                     VNRFKIERVDPEQEAA"
BASE COUNT          229 a          313 c          239 g          352 t
ORIGIN      
        1 ctcggggcgg gtatctgttg cggtgtaatg ttttagggta ttaaacgggt aggtttgctg
       61 gtcgtaacct tgccgtaacc ttgcggtaat ctctacaaaa ccttaccccc ttaaggtgtc
      121 caatatttcc accatcacgc gctctaaggt gtccaatatt tccaccatca cgcggcttta
      181 ataccctaaa ccttggtgat ttgacggcca ctagctgcac gccttgccgc cttctgtgct
      241 cgctctgctg ctgtccaata ctctggcctt ggctttgcct cacccaatag caaacgttca
      301 agggtgcggc cgccgcattc gtatggctgg cgctctttag ctatcaactc cacaagctca
      361 ggcgctgcgt aaaagctcaa acgcttaaac ccattggccg ccatcctttc gcggtatcgt
      421 tttaatctct ctgcattgct gcccatgttt tagtgcctct ttttttatcg agtaagaaaa
      481 ttattatgct ggcgcgtcac ttgttaagtt aacgcctcac ctgttctttt cctgttgtta
      541 tcaggctggt tagtatttgc cccgcttgtt tagtcgctgt gcctcgcgta gtgactccga
      601 taaaatctta gccttgcttg gtgtagtgcg gtttgtgcgg ttaaaacgga cgtttttacc
      661 ctgccccata tatatggcta cttagtattt cttttacagc taaggtaatt aaaccgttta
      721 aaccgtttaa accccccctt ttcacaaaaa cgccccaatt aaggggcgct gtatgtttat
      781 gctgcttctt gttctgggtc taccctctca attttaaatc tgtttacttt gccgcctgtg
      841 cgtttaacca gcttacggcc tcctgctata cggtctacgc gatagccaag cacacgccct
      901 aaaccgcgtg acgttaaatc attagcgccg gttagatcct gtagcaactc ttttaagtcg
      961 ccgccatcgc caccatcagc gcgcatatct gaaaagccat agatagggtt attcagtttt
     1021 ttgaacagct cgcgggtttc gaaccagcca tcccccataa gtttttctat gccctcgagc
     1081 gcctcggtta atacctcaag ctctgggtct gttgctacgg cctccttgag cgc
//
LOCUS       DASRMS010000079         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_421922, whole genome shotgun sequence.
ACCESSION   DASRMS010000079 DASRMS010000000
VERSION     DASRMS010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_421922"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..448
                     /locus_tag="VFC74_03325"
     CDS             <1..448
                     /locus_tag="VFC74_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24403.1"
                     /translation="GGGMEFSRDTEGLAYYLSKRIQRRCSMDNENQIFELMTKMYAEM
                     QKGLSDVRTEMQKGLGDVRAEMQRGFKEVNDKIQQNSDEIQFVKGTVVKIETEHGHKL
                     GALFDGYKLNAEKLDRIEKNQKTQDEKIDIIERVVTKHDVLIKNIR"
     gene            1005..1316
                     /locus_tag="VFC74_03330"
     CDS             1005..1316
                     /locus_tag="VFC74_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24404.1"
                     /translation="MGENDLASLAKLMFLSEAEQELLGQGARGEALLVVAASVCEQKL
                     SWLGTRKSCLGMGVVANGRYSHGARENPSCYHSGCIYAGDLLGGIFRSSGGHGAYSAY
                     G"
     gene            1309..1950
                     /locus_tag="VFC74_03335"
     CDS             1309..1950
                     /locus_tag="VFC74_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24405.1"
                     /translation="MAEAKQVEYYLQAASEVNEKYGLEVKWQEILALDTVLLGQNFSQ
                     SSVERAKRLAAEFVQKEVVETEEVEAKGNVLYKQVSLDKVMAAYRLSSEQKDQVSLFL
                     KMIGSREGVCMFSIIASPNYFEEIKTVITGRGSILYGKSVNDEDVIKAFDKAGRVNAS
                     VLILDVDAGGCRGYCHGGQKVQSNQTTYPNYSPCAWQEAGRFGYIPIVGQGCI"
     gene            1985..>2449
                     /locus_tag="VFC74_03340"
     CDS             1985..>2449
                     /locus_tag="VFC74_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24406.1"
                     /translation="MEIKSILEVMLEQEAATYGDAVRWDVEVVSGTSEDKKQGQMPVK
                     VQTQTIIKERYIGTGFISVVGVRRGVGCTTMAAAVANYLAAEGKKKVALVELTQYAAL
                     QWSVKLHKNVDVYTQSNGGSVKLYHDDVKNLLEEIPCQDYDFIVLDLGIVFEP"
BASE COUNT          721 a          352 c          707 g          669 t
ORIGIN      
        1 ggggggggga atggaattta gtcgggatac agaaggattg gcatattatc tgtcgaaaag
       61 aatacagcgg aggtgtagta tggataacga aaaccaaatc tttgagctta tgaccaaaat
      121 gtatgctgaa atgcagaagg gtttgagtga tgtccggact gaaatgcaga aaggtctggg
      181 tgatgtccgg gctgaaatgc agagaggctt taaggaagtt aatgataaga ttcaacaaaa
      241 cagtgatgag attcaatttg taaaaggtac tgtggttaag attgaaacgg agcatgggca
      301 taagttaggg gctttatttg atgggtataa gcttaatgca gaaaagctag atcgcataga
      361 aaagaaccaa aaaacacagg atgaaaaaat tgacatcata gagagagttg ttacaaaaca
      421 tgatgtgctt ataaaaaaca ttagataggg cttatattaa aataacgggt atgatgttcg
      481 acctgcagaa tgcttagttt agagtgttca tgcaggtttt ttagtatgaa aaaataaatg
      541 ataaagtttc tttatgtgga tgcactgtac gcaaattgtg tttcaaggtt gggtcgggga
      601 ttttttcccg caggatgaaa aacacatttt tgcttttgtt gttgtattta tttcttgtgt
      661 gtttgcttag aaaaacggat cgcagtcggg gtattgcagg tacgaaaatg gtaatactat
      721 ttcgcctgtt tttctagtgc aggataaggg ggttccacat attgctggca gtgccagcaa
      781 agacagttaa tttcaggggg tacataccgg gaataccact gctatatgtg gtacattttt
      841 gtatttacca aggagttctc tgtgtttaca gggggaacat ttgctaaagg aggtgaaaag
      901 gtgactgtaa acaggcattt ttgtgaagaa ataaaatttc gggtaactta tagcgttatg
      961 ggcaggcact agttgataac ccgtgttaca aggtgctgat gggtatgggg gaaaatgact
     1021 tagcgtcatt agcaaaactc atgtttcttt ccgaagcaga gcaggagctg ctggggcaag
     1081 gtgcccgggg agaagcgtta cttgtggtgg cagcaagcgt gtgcgagcaa aagttgagct
     1141 ggctgggcac gagaaagagc tgtttgggga tgggggtggt cgctaatggc aggtacagcc
     1201 atggtgccag agagaatcct tcgtgttatc attctggctg tatttatgcc ggtgaccttc
     1261 ttggcggtat ttttcgcagc tccggcggtc atggtgcata cagtgcctat ggctgaggca
     1321 aagcaggttg agtattatct gcaggcggca agtgaggtaa atgaaaaata tggactggaa
     1381 gtgaaatggc aggaaatatt agcattggat acggtgctgc tgggtcagaa tttttcgcag
     1441 agttcagtcg agcgggcaaa gaggctggca gctgaatttg tacagaaaga ggtggtggaa
     1501 acagaagagg tggaggcaaa gggaaatgtt ctttataaac aggtttccct ggataaggtg
     1561 atggcagcat acagattaag cagtgagcaa aaagaccagg tttccctgtt tttaaagatg
     1621 attggcagca gagagggggt atgtatgttt tctataatcg caagtccgaa ttattttgaa
     1681 gaaatcaaaa ctgtgataac aggcaggggc agtattttat atggtaaaag tgtgaatgat
     1741 gaggacgtta tcaaggcttt tgataaggca ggcagggtta atgcttctgt attgatcctg
     1801 gatgtggacg ctgggggctg ccgtggatat tgtcatgggg gtcaaaaagt tcaaagtaac
     1861 cagaccacat acccgaatta ttctccttgc gcctggcagg aagccgggag attcggttat
     1921 atcccaattg ttggccaagg gtgtatatga tatcttggct ccggaaatac ctgaagaagg
     1981 agacttggaa attaagtcaa tattggaggt aatgctcgaa caagaagctg caacatatgg
     2041 tgatgctgtc cgctgggatg ttgaggttgt aagtggaacg agtgaagaca aaaagcaggg
     2101 acagatgcct gtaaaggtgc agacgcaaac gattattaag gaaagatata ttggcactgg
     2161 ctttatcagt gttgttggtg taagaagagg tgttggctgt acgaccatgg ccgctgcagt
     2221 ggccaattat ttggcggcag aaggtaaaaa gaaggtggcc ttagtggagt taactcagta
     2281 tgcagctttg cagtggtctg ttaaactaca taaaaatgtg gatgtctata cacaaagcaa
     2341 tggcggatcg gtgaagttat atcatgatga tgtaaaaaat cttctggagg agatcccttg
     2401 ccaggactat gattttattg tcttggatct gggtattgtt tttgagcca
//
LOCUS       DASRMS010000080         1760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_556542, whole genome shotgun sequence.
ACCESSION   DASRMS010000080 DASRMS010000000
VERSION     DASRMS010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1760
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_556542"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..511
                     /locus_tag="VFC74_03345"
     CDS             50..511
                     /locus_tag="VFC74_03345"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK24407.1"
                     /translation="MITIGERLRILRAKKNFSQEYMAEALGISRPAYVNYETNRTKPV
                     RRLPELARILGVTPDYLLGYEKSGSSAQSDASNIIQLALKDYPEVLKAIESADFATNT
                     INLGDATINIDDIDLDYIKTSILSAIVMAKQKGENGKLEITVSPKAKIPDT"
     gene            complement(742..1074)
                     /locus_tag="VFC74_03350"
     CDS             complement(742..1074)
                     /locus_tag="VFC74_03350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24408.1"
                     /translation="MQKESRYHYKQAIEETIAHNDTNCIIIEHFHQIIKPAQEPRQRI
                     ELNVTYGMAQSEKDTAHEKAVQAWLAWEEDTADMYEHLREQMPDCKLWCRLKRASEKE
                     IKHIEDMM"
     gene            complement(1197..1595)
                     /locus_tag="VFC74_03355"
     CDS             complement(1197..1595)
                     /locus_tag="VFC74_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24409.1"
                     /translation="MIEAYATALSVTSSSGPISFSNKMTRGCSVVLDDDNKTFKFNLP
                     GVYKVDVSITGNVTAGGILSAQIYADGNPIARAQAQAVTGAAENQNIAFSTLLTVSPT
                     MLGERATMTLNYGGGAGALLLADVIITKIA"
BASE COUNT          496 a          404 c          374 g          486 t
ORIGIN      
        1 ctatataata gtttaaatag cttattgaaa cggggaggtt gaggaatata tgattacgat
       61 tggagaacgc ttacggatct tacgagcaaa gaaaaacttt tctcaagagt atatggcaga
      121 ggcactcggt atatcccgcc ctgcttatgt taattacgaa accaacagaa caaaacccgt
      181 aagaaggctt cctgaattag cgcgtatatt aggtgtaaca ccagattact tgcttggcta
      241 cgaaaaaagt ggttcgtcgg cacaaagcga tgcaagcaac atcattcaat tagctttaaa
      301 ggattaccca gaagtcttaa aagctatcga atcagcagat tttgccacca acaccattaa
      361 tttgggtgat gctacgataa acatagatga tattgacctt gactacatca agacctccat
      421 actgtcagct atagtcatgg ccaaacagaa aggtgaaaac ggaaagcttg aaattacagt
      481 atctccaaaa gccaaaatac ccgacacgta atttttcatt gccaagcacc agcttgagaa
      541 tagagaagga gaaaggacca tgccagacat tgtgattgtc atcgatttat ctcagctgaa
      601 cctagctgac attggtgcag atgcattagc ctaagcaaga ttatgagaag gacctgtttg
      661 gccgtaataa cggcaggaaa aaattgacat gcatatggct aagcgcaaag gaacccaccg
      721 gctatgccgg tgggttgctt tttacatcat atcttctatg tgctttatct ctttttcgga
      781 ggcacgcttc agcctgcacc acaatttgca atctggcatc tgttcgcgca ggtgctcata
      841 catatcagct gtatcttctt cccatgccag ccatgcctga acagcttttt catgcgccgt
      901 gtctttttct gattgcgcca ttccgtaagt gacattcagc tctatgcgct gtctaggctc
      961 ctgggcaggc tttatgatct gatgaaagtg ctcgataatg atacagttgg tatcattgtg
     1021 agcaatagtt tcttctatag cctgcttata atgatacctg ctttcttttt gcatcccatc
     1081 agtttataag ccatcgccaa atcaaagtga agcattatgc cattaagctg atgagacagc
     1141 actttgctca attctacttc gctggttgcc gtcagtttca ttttcagcac cgccttttat
     1201 gctatctttg tgatgatcac gtcagctagg agcagggcgc cagcgccacc gccataattc
     1261 aacgtcatgg ttgctcgttc gcctagcata gtaggtgaaa ctgttagcag ggtgctgaaa
     1321 gctatattct gattttcagc agctcccgtt actgcctgag cctgagctct tgcaatcggg
     1381 ttgccatcag catatatctg agcagacagg ataccgccag cagttacatt acccgttata
     1441 gatacgtcaa ccttataaac tcccggcaga ttaaatttaa aggtcttatt atcatcgtcc
     1501 agtacgacgc tgcaccctct cgtcatctta ttgctgaatg agataggccc gctggaagag
     1561 gtcacgctca aggctgttgc atatgcttct atcatcttaa tcagtccttt cgtaagtaaa
     1621 aagcgggcat accttccgga gaaagcacac ccgcccccat taattaaaca atggcagtac
     1681 cagtatagct ggtgtatggg ctagacacaa tatatgctgg cataggagaa ggcctcagag
     1741 caccgacaat agtctgtgtc
//
LOCUS       DASRMS010000081         9242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_690773, whole genome shotgun sequence.
ACCESSION   DASRMS010000081 DASRMS010000000
VERSION     DASRMS010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690773"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..954)
                     /locus_tag="VFC74_03360"
     CDS             complement(<1..954)
                     /locus_tag="VFC74_03360"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK24410.1"
                     /translation="MICNYCEWRCDLARGEGQCQMYTVKDGIIQEKYPYTWSRCQGEE
                     MEKVPIFHFLPDERILQLGSFNCNASCAYCINANIADPQQELVTFELQPEQIVELAKR
                     GQYKVIHFGINEVTVSLPSALKIVKLAKENGIIAGCSTNGYFTEETAKIAAEAFDFFN
                     ISLKSLQDSFYRQHLGLPSVAPVLRNIRYLAQHAHVEITTPITKDNEEEIPTIAAWLA
                     AVNPKIPWHLFRLLPANKFEHATPPSVMTLVNTVAKLADKPTYIYISNFIGSKWVDTL
                     CPHCGETVITRLSEGSCGARLQHNYLNQDLCPNCGTKQEIIV"
     gene            complement(956..1543)
                     /locus_tag="VFC74_03365"
     CDS             complement(956..1543)
                     /locus_tag="VFC74_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24411.1"
                     /translation="MYKKVSILLLVLLMVFTLVACSSPNSKENSNDPANNAGQQETPP
                     AADQPSEFQKQQELEADGKGEFKNMLQEGGTAVDEFSLKIGEHTFTEKDAAELTVYTA
                     EIEILTKVEAHQSTYVGYKLLDVLALYDLSVDADFYVVAADGYKITCKSDSLDENTLI
                     ALSKDGSTKNAPIYAPCSSKLSPNYVTGFAELRLN"
     gene            complement(1561..2340)
                     /locus_tag="VFC74_03370"
     CDS             complement(1561..2340)
                     /locus_tag="VFC74_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005814366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24412.1"
                     /translation="MIYEVNNISFAYPNTEKLILNEVSFSLARGEILSILGPNGAGKS
                     TLLNCLAGLVKPTHGEILLDGKDISDLPGKNIAQKIGYVQQAHTPAFSYSVFEFVLMG
                     RAPKISFFGRPQKKDEELVWQTLEIMKITHLANRPYTQLSGGERQQCVIAHVLVQEPQ
                     AILFDEPTASLDYGNQARILRLIKRLSAQGYAIIMTTHNPDHAMLLGGKTALFAQAGK
                     LSCGKTSEIITEESLQKIYHTDVRLLYVDALQRQACLHAKL"
     gene            complement(2352..3386)
                     /locus_tag="VFC74_03375"
     CDS             complement(2352..3386)
                     /locus_tag="VFC74_03375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZK24413.1"
                     /translation="MIIQSERELEHKHKRQARLINIALLILLFTTALIALCIGKYPLK
                     PLSALAVLFGKANLHDVMDYHVVLGLRLPRIIASIICGAALASSGTAYQGIFKNPLVS
                     SEFLGVSQGACVGAAIAILLSASAAQIQLFAFLGGLLAVGLTLLIPALMRNNSNLMLV
                     LSGIIIGGAMSSVLGFIKYIADPETELAAITYWQMGSFSYITSDQLASVLLPMGLASL
                     CLFGLSWRIDILSLGESEAQTLGTNVPLVRIFTIICATLLTASSVSIAGTISWVGLII
                     PHFGRILVGTGNTRLLPTACLGGALFLLTVDTVTRTATAAEMPISILTGLIGAPLYAW
                     LLYKRRMIIN"
     gene            complement(3383..4405)
                     /locus_tag="VFC74_03380"
     CDS             complement(3383..4405)
                     /locus_tag="VFC74_03380"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK24414.1"
                     /translation="MLRKTIAALLLVLLVFTTACAKIPQQTVAGTVKTVTDQAGRQVK
                     LPDDVETVAMMFSIPTSLLLALGVGDRLVAVGATWGIQTLIEPSLETIGTVGRGQLDM
                     ESLAAYNPDVFLHKASDPATLEVVAKLGIPALGLTTESTAEVLEAITVLGEVFNRQAR
                     AAELVEYYHSKMELANRIVAQIPADERKTAIVMGSEIGKVAGGNMLQSFMIETAGGIN
                     CAQDVESQQAWPVVGTEKIFAWNPDFIFCTNGSASEYEAADLLHDPTWQNMNAVKNNR
                     VLLVPTDKDSWEYPSLSTCLGFLWMLCQMYPEYYSEADFLQEVDLFYQTAYGMTFNLE
                     FLGYRT"
     regulatory      complement(4537..4655)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            5043..6212
                     /locus_tag="VFC74_03385"
     CDS             5043..6212
                     /locus_tag="VFC74_03385"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:NF014098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HZK24415.1"
                     /translation="MSRLPDYIGPLAFGVKIGVVVPKIDLVKMITAALAEVARDELLD
                     DGDVICVTESVVARAQNNFVTVTEIAEEVRRKLKVSKAGKIAIVFPITSRNRFSLILQ
                     GVAAAVPQGEVVVQLSYPSDEVGNQVITQELAEELTAEKDYIMYEDLQEEDCLHPITK
                     VNYLTLYKKIIEGQGAKATIFLSNNPQHITKFNPDGVIVADVHGREATSKKLSKVYSN
                     FITLQDLCREGESWSEWGLLGSNMSANDQLKLAPRESSVFVRELQAAIKETTGRKVEV
                     LVYGDGAYKDPSTGIYELADPKPVFGMTDGLAGRYREGIKYKYVADVCYAAGKSVEEI
                     EAQLAAQKQEAYQHNDILTEGTTPRRVEDVIASLADLVSGSADAGTPVVVVKGIL"
     gene            complement(6450..6617)
                     /locus_tag="VFC74_03390"
     CDS             complement(6450..6617)
                     /locus_tag="VFC74_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24416.1"
                     /translation="MPKGGFGGGDNNFLLLFLLLLLLGDNGGGEDNMLLIILFIILFS
                     ENGFGGRTELS"
     gene            complement(6755..7081)
                     /locus_tag="VFC74_03395"
     CDS             complement(6755..7081)
                     /locus_tag="VFC74_03395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24417.1"
                     /translation="MSDFRQDLYSLLSNVNSDDLLENINKVGMLLGDPKQTTNVKKVL
                     RSFLTEERDDPAVKLLVALTPFLSKNKQARVNEYVKVLNTSNLFNVIQQIQQEQIILQ
                     QDTQEE"
     gene            complement(7115..7591)
                     /locus_tag="VFC74_03400"
     CDS             complement(7115..7591)
                     /locus_tag="VFC74_03400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24418.1"
                     /translation="MDIVLGYRKRKAAATASAEELNTTADARELEQAEEDKGLEAGKD
                     TVEPVEEALTSTPVSKEEQAENLPAEGARETVKAKLNPAKITEIEKAAAVSAVSPQKT
                     IVKKILSEALSSKKPTLPNLPKNPFDIPDENNNPASTVNDDSLLLLLILILLKTRK"
     gene            complement(7675..8013)
                     /locus_tag="VFC74_03405"
     CDS             complement(7675..8013)
                     /locus_tag="VFC74_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24419.1"
                     /translation="MINNLDVGALLAKLNNGELTENINRLSQLLNAPETDENEEQAIS
                     ASITDNAADPLNKEAYVNHHDPRINLLIAITPFLSTAKRNKAEMLLKPLNLLYFLRQF
                     DSLAEQVEKS"
     gene            8174..8977
                     /locus_tag="VFC74_03410"
     CDS             8174..8977
                     /locus_tag="VFC74_03410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24420.1"
                     /translation="MMEILFTLTPAEAKRLLAKAVVQLPAVQKAFKYGRLAIVNGTTN
                     AFVVEELLHETISKERFTYGVITAGRTCSNDPDKALKPYLFEQGQRVEANLKEFLAAL
                     QGADVVIKGANAVDHEGNIGILLGSRDGGTIGAAWPTLQAVGATLIVPVGLEKLVPAV
                     RQVPASVGIDNYAHSLGMPVGYMPITTKATVVTEIEALAILGKVEATLIAAGGTGGSE
                     GSTTFVVAGTEERVEKVWQLIKQIKGEPPVRRARQACANCSVRCDACHD"
BASE COUNT         2487 a         1892 c         2177 g         2686 t
ORIGIN      
        1 cacaatgatc tcttgtttag tgccgcagtt ggggcagagg tcttggttta agtagttatg
       61 ctgcagacgg gcaccacatg atccttcgga caaacgtgtg attacagttt caccgcagtg
      121 gggacagaga gtatccaccc atttggagcc gataaagtta ctaatgtaga tgtaggtggg
      181 tttgtctgct agtttggcaa cggtattgac cagtgtcatc acagaaggtg gagtggcatg
      241 ttcaaattta ttagccggca gcagcctgaa gagatgccaa ggtattttag ggttgacagc
      301 agcaagccaa gcggctatag ttggaatttc ctcttcgttg tctttggtaa tgggagtagt
      361 tatttctacg tgagcgtgtt gggcaagata gcgaatgttt cttagtacgg gggcaacaga
      421 gggcaagcct aaatgctgcc gataaaaact atcttgcagt gattttaggc tgatattaaa
      481 gaagtcaaat gcttctgctg cgatcttggc tgtttcttct gtaaagtagc cattagtgga
      541 acagcccgca atgatgccat tttccttggc cagtttgacg atcttgaggg cagaagggag
      601 acttaccgtg acttcattga tgccaaaatg gatgacttta tactggcctc gtttcgccag
      661 ctctacaatc tgctctggct gcagttcaaa ggttaccagt tcctgttgtg ggtcggcgat
      721 attggcattt atgcagtagg cgcagctggc attgcagtta aaggagccaa gctgtagtat
      781 ccgttcatca ggcaggaagt gaaagatggg gactttctcc atttcttcac cttggcagcg
      841 tgaccaggtg tatggatatt tttcttgaat gatgccgtcc ttaacggtat acatctggca
      901 ctggccttct ccacgggcaa ggtcacagcg ccattcacaa taattacaga tcattttagt
      961 tgagcctcaa ttctgcaaaa cccgttacat agttgggtga aagcttactg gagcaaggtg
     1021 cataaatggg ggcatttttg gtgctgccat ccttgctgag ggcaattagg gtgttctcat
     1081 caagggaatc acttttacag gtgattttat aaccatcagc ggcaacaaca taaaaatcag
     1141 catcaactga gaggtcataa agagccaaaa cgtctaggag tttatagccg acatatgtag
     1201 actgatgggc ttcgactttt gtcaagattt ctatctcggc agtatagacg gttagttcgg
     1261 cagcatcctt ttcggtaaaa gtgtgctccc caattttcag gctaaactca tctaccgcag
     1321 ttccgccctc ttgcagcata ttcttgaatt ctcccttgcc gtcggcttct agttcttgct
     1381 gcttttgaaa ttctgaaggt tgatctgcag ccggtggtgt ctcttgctgt ccggcattat
     1441 ttgcaggatc atttgagttc tccttggagt taggtgagct gcaggctacc aaagtaaaaa
     1501 ccattagtaa aactagcaat agtatcgata cttttttgta cattattttt ctcctccatt
     1561 ttataatttg gcatgcagac atgcctggcg ttggagtgcg tctacatata gcagccgcac
     1621 atcggtatgg tagatttttt gtaaactttc ttctgtaata atttcgctgg tcttgccgca
     1681 gcttaacttg ccggcctgag caaagagtgc tgttttacca cctaggagca tagcatggtc
     1741 ggggttatgg gtggtcatga tgatggcata accttgcgcg gataatcttt ttattaaccg
     1801 caggatgcga gcctgattgc cataatccag ggaggctgtt ggttcatcga acaagatggc
     1861 ctgtggttcc tgtaccaaga catgtgctat gacacattgc tgacgttcac caccactaag
     1921 ttgtgtatag gggcggttag caaggtgagt gatcttcatt atctccaatg tttgccatac
     1981 cagttcttca tccttttttt gtggtcggcc aaagaagctg attttaggtg ctctacccat
     2041 taacacaaat tcgaacactg aataagaaaa ggctggggta tgagcttgct gtacatagcc
     2101 gattttctgg gcaatatttt tgcccggcaa atcgctgatg tcttttccat ctaggagaat
     2161 ctcaccatga gtaggtttaa ctagtcctgc cagacagttg aggagagtac tttttcctgc
     2221 accgttgggc ccaagaatag agagaatttc tcccctggct aaggaaaaac ttacctcatt
     2281 taagattaat ttttcagtgt tggggtaagc aaaactgatg ttatttactt catagatcat
     2341 tgcacatcgc ctcaatttat tatcatccgc cgcttatata gcagccaggc atataatggt
     2401 gcaccgatta gtccagtcag aatactaatg ggcatctcag ccgcagtggc tgttctggtg
     2461 actgtgtcaa ctgtgagcaa aaacaaagcc ccgccaaggc aggctgttgg cagcaaccgt
     2521 gtatttccgg tacctaccag gatacggcca aaatgtggga tgatcagacc tacccaactg
     2581 atagtgccgg caatggaaac agaactggca gttaacaagg tcgcgcaaat gatggtaaaa
     2641 attcttacca gcggcacatt agtgcccagg gtctgagctt cactttcacc taatgaaaga
     2701 atatcaatcc gccaggacag gccaaataag cacaaagagg ctaatcccat tggcaagagc
     2761 acacttgcta gttgatcgct ggtgatatag gaaaaactgc ccatctgcca gtaggtaatg
     2821 gcggcaagtt cagtttctgg gtcggcgatg tatttaataa aaccaagtac cgagctcatc
     2881 gcgccgccga taataattcc agatagtaca agcattaaat tggagttgtt gcgcatcaaa
     2941 gcaggtatga gcagtgtcag tccgacagct aaaagaccgc cgaggaaagc gaaaagctgg
     3001 atttgtgctg ctgaagcgct taacagaatg gcgatagcag ctcctacaca ggcaccttgc
     3061 gagactccta aaaattcaga tgagaccaga ggattcttga aaattccttg ataggctgtg
     3121 ccggagctgg caagcgctgc tccgcaaatg atcgaagcaa tgatccgtgg taaacgaagg
     3181 cctagtacaa catggtagtc cataacatcg tggagattgg ccttaccaaa gaggaccgct
     3241 aatgcggaca gtggtttaag gggatatttg cctatgcata gcgcgattag ggcagtggtg
     3301 aataataaga tgagtagggc tatgttgatc agccttgctt gccgcttatg tttatgttcc
     3361 aattctcttt cactttgtat gatcatgttc tataccccaa aaactctaaa ttaaaggtca
     3421 tgccgtacgc tgtttgataa aacagatcaa cttcttggag aaaatcagct tcactataat
     3481 attccggata catttgacat aacatccaaa gaaagcccag gcaggtagac aagctgggat
     3541 attcccagga gtctttgtct gttggtacta acagcacccg gttattttta actgcattca
     3601 tattttgcca ggtagggtca tggagaagat ctgctgcttc atattctgaa gctgaaccat
     3661 tggtacagaa gataaaatcg gggttccaag caaagatttt ttctgtgccc acaaccggcc
     3721 aggcctgctg actttccacg tcctgggcac agtttatacc gcctgctgtt tcgatcataa
     3781 aggattgcag catattgccg cccgccacct tgccgatttc agaacccatg acaatagcgg
     3841 ttttgcgctc atcagcaggg atttgtgcta caatacgatt tgccaactcc attttgctgt
     3901 ggtaatactc aactagttcc gctgctctgg cctggcgatt aaaaacttcc cctaatactg
     3961 tgatggcttc cagcacctct gcagtacttt ctgtcgttag cccgagggca gggatgccta
     4021 gcttggcgac tacttctagg gttgcggggt ctgaagcttt atgtaaaaac acatcaggat
     4081 tgtaggcagc caaggattcc atatctaact gtccgcgacc aactgtaccg attgtttcta
     4141 gtgatggctc gatcagagtt tgtatgcccc aggttgcacc aacagccacc agcctatctc
     4201 ctacgcctaa agcaaggagc agggaagtgg ggatactgaa catcatcgcc acagtctcta
     4261 catcatcagg caatttcacc tgccggcctg cttgatcggt tactgttttt acggtgccag
     4321 ctacagtctg ctgagggatc ttagcacaag cggtggtgaa aactaacaat actaatagta
     4381 aggctgcaat tgtttttctt aacatctctg ctcacctcaa tctcatttaa agcagcaaaa
     4441 atttgctgtg aagcaagaat actatttgca tacaaataaa gcagatcctc ccataatata
     4501 agagagacct gctttaatct aaagaatctg ttttttcaca gttagataag cgctctttcc
     4561 aaatacggga gacgcagaag ctttccctct gcacccatag gtcgggcacc atagaaaaat
     4621 ccttaggcat aaccgacttc aagccacttt cctcattaat attatagcag ttcaaaggat
     4681 aggcggcaat atgttttctt ctcataggaa acacgcggct gcagataaac ttctttgagc
     4741 caaaaacatg tagtctctac cttgattttt aagtgcatat cagtcataat actagaggga
     4801 agtagatgcc tgaactgcaa ctgatgaggg cgctaaactt agttgatttg caggcattga
     4861 ttacccggtc taaaattagc tgtatgaaaa ttcctctttg cgccctcaga tgatagcgaa
     4921 gaggtttttt gttgagcagc ctacatagcg agctgtggtc aaagttttat ttcactttac
     4981 tatcataaag tggtaaaata actgtggtct aagttgcagt ttaacagagt ggaggaatca
     5041 aagtgagtag attgcctgat tatattggtc ctttagcttt tggtgtaaaa ataggtgtgg
     5101 ttgtccccaa gatagattta gtcaaaatga tcacggcagc actagctgaa gtagctcgtg
     5161 atgagctgct ggatgatggt gatgttattt gtgtgaccga atccgttgtc gctcgtgctc
     5221 agaataactt tgtgacggta actgaaatag cagaagaagt acgacggaag ttaaaagtca
     5281 gcaaggctgg gaaaatcgct atagtttttc caattaccag ccgtaatcgc ttctcgctca
     5341 ttcttcaggg tgtagctgca gcagtaccgc aaggcgaagt tgtcgtgcag ctctcctatc
     5401 cgagtgatga agtaggcaat caggttatta cgcaagagct ggcggaagag ttgactgcag
     5461 aaaaagacta tattatgtat gaagatttgc aggaagaaga ttgcctgcat ccaattacca
     5521 aggtaaacta tctgacctta tacaaaaaaa ttattgaggg acagggtgct aaagcgacaa
     5581 tctttttaag taataaccct caacacatca ccaaatttaa tccggatggt gtaattgttg
     5641 ctgatgtcca tggtcgtgaa gcaacaagta aaaaattgag caaagtatac agcaacttta
     5701 ttaccctgca ggatctttgc cgtgaggggg agagttggag cgagtgggga ctgttaggca
     5761 gtaatatgtc agctaacgat caactaaaat tagctccccg cgagtcatca gtctttgtgc
     5821 gtgaactaca agcagcgatc aaagaaacta caggacgtaa agtggaagtg cttgtctacg
     5881 gcgatggtgc ttacaaggat ccgagcactg gtatttatga actagctgat ccgaaaccgg
     5941 tttttggtat gacagatggt ttagctggac gctatcgtga aggcattaaa tataaatatg
     6001 tagctgatgt ttgttatgcg gcaggaaaaa gcgttgagga aattgaagct caattggctg
     6061 cgcaaaaaca agaggcttat cagcacaatg atattctgac tgaaggaacc acaccgcgtc
     6121 gggtggagga tgttatcgct agtttggctg atttggtcag tggctctgct gatgccggta
     6181 caccggtagt ggtggtcaaa ggtatcttat agggaaaaaa atccgcagtg atgcggattt
     6241 tttatataca gcaatagatt agttctcttg ctagctagta gggcgctaat gtaagcagta
     6301 taaaaagctg gcactcggaa actcaaagtg ccagcttttt tatgtaagcc ctgtgtaatg
     6361 cacagggcaa aacagggctt ttaaactatg cagtaagatg cgatgctttc aggtgtgtct
     6421 cttgcatatt gtctgataca tttactaatt taactaagtt cggtacgacc tccaaaacca
     6481 ttttctgaga agaggatgat gaaaaggata atcaatagca tattatcctc accaccgcca
     6541 ttgtcgccca gaagtaacag caagagaaat aataagagga agttattgtc gccaccaccg
     6601 aaaccgcctt ttggcattta catttcacct cttttgctta ttgttaacgt atcttgggta
     6661 cctagcttat actatgattt atgcaactgt ttggtgatta atcagtgatt gaaaattgtg
     6721 ctgcagggca gctatactga tgatatcgaa ctatttattc ttcctgtgta tcctgctgca
     6781 gaataatttg ttcctgctga atttgctgaa tgacattaaa taaatttgat gtatttaaaa
     6841 cttttacata ttcatttact cttgcttgct tgttctttga taaaaatggt gttaatgcta
     6901 ccaacaattt aactgccggg tcatcacgtt cttctgtgag aaaactcctt aacacttttt
     6961 ttacatttgt tgtttgttta ggatcgccaa gcagcatacc gactttattt atgttctcaa
     7021 gaagatcatc tgaattgaca ttgctgagca ggctgtataa atcctgccga aaatcactca
     7081 tcgtagttac gccccccttt tacattgaat cggactactt acgtgttttt aataagatta
     7141 aaattagtaa gagcagtaat gaatcatcat taactgtact ggcaggatta ttgttttcat
     7201 ccgggatgtc gaatggattt tttggtaagt ttggtagtgt tggcttttta ctggaaagtg
     7261 cttctgacag gatctttttt acaatagttt tttgcggtga tactgcagac actgctgcag
     7321 ccttttctat ttctgttatt ttagcagggt taagtttggc ttttactgtt tctcgtgcac
     7381 cttcagcggg gaggttttct gcctgttcct ctttgctaac aggagtagaa gtgagtgctt
     7441 cttcaactgg ctctacagtg tctttgccag cttctagccc tttgtcttct tcagcttgtt
     7501 ccagttctct agcatctgcc gtagtattta attcttctgc agaggcagta gcagctgctt
     7561 tgcgtttgcg ataaccaaga acgatatcca atttgcagcc tcccttcgct aatgtgcaag
     7621 atgattaaag tgcttgttat gatttagaca accagatttt aaggtgttgg catgctaact
     7681 tttttctact tgttctgcta aactatcgaa ctgccgtaag aagtatagta gatttaaagg
     7741 ttttagcagc atttctgctt tgtttctttt tgcagtggag aggaagggcg taatggcaat
     7801 aagtaagtta atcctggggt cgtgatgatt gacgtatgcc tctttgttta gcgggtctgc
     7861 tgcgttatca gtgatagagg cgcttattgc ttgttcttca ttttcatctg tctcaggggc
     7921 gtttagtaac tgactaagac ggttgatgtt ttccgttagt tcaccattat tcagtttagc
     7981 taaaagtgca cccacatcca aattgttgat catgctcgtt tactcccttt tagaataaat
     8041 acaactttct tctgatatct tatgtgagaa gaaagaaaaa cgttcaagta ctgagttagg
     8101 caaaaatgat gataaaaagg aattatcaac accagcaaag aaagatagtc taacaagcaa
     8161 cagaaggggt ttgatgatgg aaattttatt tactttaacg ccagctgagg ctaaacgcct
     8221 gctggccaaa gcagttgttc agctgccagc ggtgcaaaag gcttttaaat atggcagatt
     8281 agcaattgtg aatggtacga ctaatgcctt tgtggtggaa gaattactcc atgaaacaat
     8341 ctccaaggaa cgctttactt atggggtaat taccgcaggc aggacctgct ctaatgaccc
     8401 tgacaaagcc cttaagccat atttgtttga gcagggacag cgagtggagg ctaacctcaa
     8461 ggagtttctg gcagctttgc agggcgctga tgtagtgatt aaaggtgcca atgcagttga
     8521 ccatgaaggt aatatcggca ttctgcttgg cagccgcgat ggcggtacga ttggtgccgc
     8581 ttggcccacg ctgcaagctg taggtgccac cctcatagta cctgtgggct tagaaaaatt
     8641 agttcctgcg gtgcgtcaag ttccagcctc ggtaggcatt gataattatg ctcatagttt
     8701 aggcatgcct gttggttaca tgcccattac aacaaaggcg accgtagtga cagaaattga
     8761 ggctttagca atattaggca aagtggaggc aacattaatt gccgccggtg gtacaggcgg
     8821 ctcggaaggc agtactacat ttgtggtagc gggcacagag gagcgggtag agaaagtctg
     8881 gcagctgatt aagcagatta aaggtgaacc gcctgtgcgc cgagcgcgcc aggcttgtgc
     8941 aaactgcagt gttcgctgcg atgcttgtca cgactaattc gcaatttagt taattgtgca
     9001 tgttaaaata ctagctaggc aaacaaaaat agtgcccttt gtagggggac actatttttg
     9061 tttttaggca ggggagaccc ttcaattgtt ggtaggtaag atgagacttt tacgggaaac
     9121 ttaatgttgg aaggggtcat aaaccatact gacaccacag cctttaccga ttagctcatt
     9181 tagggccatt aagtcttccc gtcctacttc agccagactc gtcttgccca gcgccttgat
     9241 gc
//
LOCUS       DASRMS010000082         1621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_221598, whole genome shotgun sequence.
ACCESSION   DASRMS010000082 DASRMS010000000
VERSION     DASRMS010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221598"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1507
                     /locus_tag="VFC74_03415"
     CDS             <1..1507
                     /locus_tag="VFC74_03415"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HZK24421.1"
                     /translation="SYREYIKTFPEGKFVSIADELIQEDESAGTFTDSRDGNVYKFVT
                     IGNQVWMAENLKYLPVVVKPSTESRTDPHYYVYDYDGTNVNAAKKTDNYSTYGVLYNW
                     PAAMARSASSTANPSKVQGVCPTGWHLPSDVEWTQLIDYLGGDEAAGGKLKETGTAHW
                     DSPNRGATNETGFTALPGGYRNNTGTFNYIGYYGTWWSATENNATDAWNRYMSHNNSA
                     VYRNDNDKEVGFSVRCVRDYATNSKGTGYGTTDSFATYQEDESIGTFTDSTDDGSKGT
                     GYGTTDSFATYQEDESAGTFTDSRDGNVYKFVTIGNQVWMAENLKYLPSFEGPATYYV
                     YGYKGTDVNAAKATANYSTYGVLYYWTTAMAGSTSSIANPSGVQGICPAGWHLPSDAE
                     WTQLIDYLGGDEVAGGKLKESGTAHWKLWERDPNVTEQATNETGFTALPGGELGYTYP
                     FEGIGSYAYWWSASEDATGTVWHRSMDYDYSGVGRGNWYKECGYSVRCVRD"
BASE COUNT          488 a          324 c          399 g          410 t
ORIGIN      
        1 atcatataga gaatatataa aaacatttcc agaaggtaaa tttgtttcaa tagctgatga
       61 attaattcaa gaagatgaaa gtgctggcac ttttaccgac tctcgtgatg gcaatgttta
      121 taaattcgta accattggta accaagtgtg gatggcagag aacttaaaat atttacctgt
      181 agttgtaaaa ccatctactg aatctaggac agatcctcat tattacgtat atgactatga
      241 tggaacgaat gtaaatgcag ccaagaaaac ggacaattac agcacttatg gtgttttata
      301 caattggcct gcggctatgg cacgttcagc aagcagtact gctaacccaa gcaaagtgca
      361 aggtgtttgc ccaaccggct ggcacttacc cagcgatgta gaatggacac agttaattga
      421 ctatttggga ggagatgaag cagcaggcgg taaactaaaa gaaaccggaa cagcacattg
      481 ggatagcccc aacagaggag caaccaacga aacgggcttt acagccctgc cgggaggcta
      541 ccgtaacaat actgggacat tcaactatat tggatactac ggtacctggt ggagtgctac
      601 ggagaacaat gcaacagatg catggaaccg ttacatgagc cacaataaca gcgctgtata
      661 caggaacgac aacgataagg aggttggttt ttccgtgcgt tgcgtgaggg attatgctac
      721 caacagtaag ggtacaggat atggtaccac cgattctttt gctacatatc aagaagatga
      781 aagtattggc acttttaccg actctactga cgatggtagt aagggtacag gatatggtac
      841 caccgattct tttgctacat atcaagaaga tgaaagtgct ggcactttta ccgactctcg
      901 tgatggcaat gtttataaat tcgtaaccat tggtaaccaa gtgtggatgg cagagaactt
      961 aaaatattta ccttcatttg aagggcccgc tacttactac gtatatggct ataagggaac
     1021 ggatgtaaat gcagccaaag caacagccaa ttacagcact tatggtgttt tatactattg
     1081 gactactgct atggcaggtt caacaagcag tatagctaac ccaagcggag tgcagggtat
     1141 ttgcccagct ggctggcact tacccagcga tgcagaatgg acacagttaa ttgactattt
     1201 gggaggagat gaagtagcag gcggtaaact aaaagaatcc ggaacggcac attggaaact
     1261 ttgggaacgt gatccgaatg tcaccgaaca ggcaaccaac gaaacgggct ttacagccct
     1321 tccaggtggc gagcttggct acacttaccc attcgaaggc attggaagct acgcttactg
     1381 gtggagtgct tcagaggacg ctaccggtac cgtctggcat cggtccatgg actacgatta
     1441 tagcggtgta ggcaggggca actggtataa ggagtgtggg tattctgttc gttgcgtgag
     1501 ggattaagtt ttaatttatt tgactatttt attatttgat tattccaccc actccccctc
     1561 gtttgcaaca gcctgccccg atgatataac gggaaggggc aatggtttag cgtttgtaac
     1621 g
//
LOCUS       DASRMS010000083         1864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_623335, whole genome shotgun sequence.
ACCESSION   DASRMS010000083 DASRMS010000000
VERSION     DASRMS010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1864
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623335"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            265..444
                     /locus_tag="VFC74_03420"
     CDS             265..444
                     /locus_tag="VFC74_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24422.1"
                     /translation="MKFADMSCHDMTTLIAFGGMVIGLLLAIVYDQKDLGYVIAGALG
                     GAISLKEGTKNYDKG"
     gene            431..1024
                     /locus_tag="VFC74_03425"
     CDS             431..1024
                     /locus_tag="VFC74_03425"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZK24423.1"
                     /translation="MIRVNLDALKDIATVSRQNVWAKAQAAGRQPAIILHWSAGYYNT
                     CFDDYHINITGDGEIKLSTRDLAQNCSHTYLRNSGSIGVSLCCAYGANTNNLGNYPPT
                     KKQIEVMAQVIAVLADALWLTIDKNHVMTHGEAADNEDGLYPCEPYGPKNGCERWDLE
                     FLGTSESPRYNPYADDGTRGGDILRGKANWYRNRSKE"
     gene            1029..1508
                     /locus_tag="VFC74_03430"
     CDS             1029..1508
                     /locus_tag="VFC74_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24424.1"
                     /translation="MELHTILELLAAALLAYAGGRLQSRANKHDAEAERREARLQELF
                     SNVEILLQYHDEMANKIEGMNSQIAELERQMGRVIKGGLSLLKNDIVRACSEAIARDE
                     ITIAERNRITEMYRIYHDEFAGNGEGEFYYNEMMEIPVVGAYTKNHGEVRYVYQAKS"
     gene            1486..>1864
                     /locus_tag="VFC74_03435"
     CDS             1486..>1864
                     /locus_tag="VFC74_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24425.1"
                     /translation="MFIKLNHDKIARYDGIAIRALTAAGFILLGLFFFYFYFFDKHEP
                     PEKETGMSQVQAETEAGINTAAHNAQVPLSATQAKEVVTQIREIHTTEQVPVYVVQST
                     GTTVRQDVEQAREDNKADFAVVTD"
BASE COUNT          528 a          346 c          515 g          475 t
ORIGIN      
        1 cagcagcagg tgtgggagcg cgttgagaaa ggtactatca ctgatgagga ataccgctat
       61 attattggcg gttaatggag aaaggttctg ggtaatatta taacggtggc aagggcggag
      121 aaatctgccc ttatttattt gaaggaggta gttattatga ataaggattg gcttttaggt
      181 atgtcattag ctgttaatgt gggcgtagga atgctcctgc tggcatggct tatcggcggt
      241 aatgcttgat aggagggtga gaagatgaaa tttgctgaca tgagctgtca tgatatgaca
      301 acacttatag cttttggcgg gatggttata ggcctcctgc tggctattgt ttatgaccag
      361 aaggaccttg gctatgttat cgctggtgcc cttggtggtg ccatcagttt aaaggagggg
      421 acaaagaatt atgataaggg ttaatcttga cgcattaaaa gatattgcta ctgtttcaag
      481 gcagaatgta tgggcgaagg ctcaggcggc aggcagacaa cctgcaatta tcctacattg
      541 gtctgctgga tactacaata cctgctttga cgactaccac atcaatataa cgggtgatgg
      601 tgaaatcaag ctcagtacca gagacttggc gcagaactgc tcccatactt atctccgcaa
      661 cagcggcagc ataggcgtta gcctctgctg tgcgtacggt gctaatacta ataatctggg
      721 gaactatccg ccgactaaga agcagataga ggtaatggca caggttattg cggtgctggc
      781 agatgcgctt tggctgacca ttgataagaa tcatgtaatg actcatggcg aggcggctga
      841 taatgaagat ggtctttacc catgcgagcc atacggaccg aagaacggct gtgagaggtg
      901 ggatctggag tttttaggca cttcagagag ccctaggtat aatccttatg ctgacgatgg
      961 caccagaggc ggtgacatcc tcagaggcaa ggcgaactgg tatcgcaatc gaagcaagga
     1021 gtgattatat ggaattacat actatattgg agcttttggc tgctgcgctt cttgcgtatg
     1081 caggcggacg cttgcagagc agagcaaaca agcatgacgc cgaggcagaa cgcagagaag
     1141 ccagacttca ggagcttttt agcaatgtcg aaatcctcct gcaataccat gacgagatgg
     1201 ccaacaagat agaaggcatg aacagtcaga ttgcagagtt ggagaggcag atgggtagag
     1261 taatcaaggg cgggttgagc cttctgaaaa atgatattgt acgggcctgt tccgaagcta
     1321 tcgcgcgcga tgaaattacc attgcggaaa gaaatcgaat aacggaaatg taccgtatat
     1381 atcatgacga atttgcggga aacggtgaag gggaatttta ttataacgaa atgatggaaa
     1441 ttcctgtagt cggagcttat actaaaaatc atggggaggt aagatatgtt tatcaagcta
     1501 aatcatgata agatagctag atatgacggc atagctattc gtgccctcac agctgctggc
     1561 tttattctgt tgggattatt cttcttttat ttttatttct ttgataagca cgagccgccc
     1621 gaaaaagaaa ctggcatgag ccaggtacaa gctgagactg aagcaggcat taatactgca
     1681 gcacataatg ctcaggttcc tttatctgca acgcaggcca aggaagtagt tacccagata
     1741 agggaaatcc atacaactga gcaggtgcct gtttatgtgg tgcagtctac tgggacaact
     1801 gtcagacagg atgtagagca ggcacgcgag gataacaagg ctgattttgc cgttgttact
     1861 gatc
//
LOCUS       DASRMS010000084        14790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_87297, whole genome shotgun sequence.
ACCESSION   DASRMS010000084 DASRMS010000000
VERSION     DASRMS010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 14790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14790
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87297"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="VFC74_03440"
     CDS             complement(<1..1174)
                     /locus_tag="VFC74_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24426.1"
                     /translation="MKQLLTMPFIFHSQTNIYLLELGQDGVLSSTRFNLADENIKRTE
                     VLVNCARFCAAVNNSEQLYILTANQQNELLSFLLADDEIEQLPFFTNAAPSAFTLAFD
                     STGLGYYCAESKDGLTFALLNETMQWQTYDIPGGQQSAPLAMAIDQAAYLHLLLFDRA
                     EKMLYYQSLEPKTYRPTKPFYLATNYTLITRPAFLFDSFQNLHLAWQEDDLLHYQARL
                     AGGWPSGGWQHKVTLPLQFSVQLMSFIEAYPQPQLWLMDEDKLVHYYNPLEETEISCS
                     SSAEILIPARFAPTTSQQIRLIKQSEETSLLVNVVKTLQEDKQVQTSRKEEEESPLFL
                     HARRLMAEKKRLEYELSKKEASLAQLRHMLELAQENLRRNSISVNEKLYEINQRSKE"
     gene            1348..2016
                     /locus_tag="VFC74_03445"
     CDS             1348..2016
                     /locus_tag="VFC74_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24427.1"
                     /translation="MKKYKGGCGGHGGGHGDDQVADVQLGQCDPIYGCPPPTEVVCLL
                     VDKVYDECKNVQVDEVEFTYEACPENPIVDVLCYKVEIIDGPCCEVVSSGRVRVTVSY
                     KVKIKLIFEDGSTQKLCEEKTVIKTFNVPRAGEPGFFLHCEVPFLECLQAFVESEELE
                     YGALRTTIIACIGKYTLLKLLATVQIAVPSYGFCPEPPDCDEVLGECPDFNPEWPPYP
                     LQDR"
     gene            2401..3555
                     /locus_tag="VFC74_03450"
     CDS             2401..3555
                     /locus_tag="VFC74_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK24428.1"
                     /translation="MRIGMFTDSYKPYTSGVVRSIETTTAKLTELGHEVYIFAPNYPN
                     CEEEEGVFRFASVPTPTYPDFSIALPFSLNLSATVKRLDLDVIHVHSPFSMGLLGSRC
                     AKRFDIPLVFTYHTMYDQYVHYLPFAQGFSKKVVLKLSCNFCNRCDLVIAPTNIVRQI
                     IAPNVVTKVKAIPTGIEISEFATLDRQWLREQYQIPHEKKILLHLGRLGKEKNVGFLL
                     QSYRMIRQTQPETVLVIVGDGPDRESLENQVNEMGLSQTVIFTGTMPREQVVNAYAGA
                     DLFIFASTTETQGLVLGEAKAAALPSVAVKALGAAEMVKDGIDGFLTTLSLDDFTARV
                     QQLLQDEQLRQAMAARCVEAAEEISSINMAKKLVTAYEGAINDKQCRDVG"
     gene            complement(3670..3846)
                     /locus_tag="VFC74_03455"
     CDS             complement(3670..3846)
                     /locus_tag="VFC74_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24429.1"
                     /translation="MEVKSFLERAMLNEQEQVRDYQRFATQTDNDEVRKAFFEFAEIS
                     GHTATKIKDLLDNL"
     gene            3986..4372
                     /locus_tag="VFC74_03460"
     CDS             3986..4372
                     /locus_tag="VFC74_03460"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HZK24430.1"
                     /translation="MKKLYRSRDQQMLGGVCMGIARYFNVDVTLIRLLWVIGGLAGGG
                     IGLPAYIIAWIIIPEEPGIGEAIDISSSEGEAPNIDSRTVGLVVIAVGVFLLIRQFLP
                     LSIFRYYLWPLAFIGVGLLLVWGGRK"
     gene            4369..5250
                     /locus_tag="VFC74_03465"
     CDS             4369..5250
                     /locus_tag="VFC74_03465"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HZK24431.1"
                     /translation="MKADRIVLGLAIVLIGLMWLLVNLGVVPAVTVRQLWRYWPLLLI
                     LWGVLLLLGKDNSGVTGFLVVLLVLALVGGGFFTHFTFSGPNNAETFETTISNSDNVQ
                     AVKMQLIQHAGELNLASHQGDELAELKLQASAQPSVSHKQAEEIAEIIIEDQSNNLNF
                     DKKVSRWELSLAEKLPLQLSLQTGATKANLDFSHLLLKKLEIEAGAGDIDLKLGQSDC
                     EITIESGASNITIRIPEAVGVRLRTSGALMSVDSDEGRMISVGDRSYDSKNLETKEAI
                     ADIHIAAAAGNVTLRHD"
     gene            5403..5882
                     /locus_tag="VFC74_03470"
     CDS             5403..5882
                     /locus_tag="VFC74_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012705661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat protein"
                     /protein_id="HZK24432.1"
                     /translation="MMQLTQKERMLLEDQKNQEELCIKKYTEAAQKAKDPQLQQLFTT
                     YADQERQHYQTVEQIMQGQVPSTQPGSQAGQQSGQQQSGQNMPGGTTALASQSDADLC
                     NDMLSTEKYVSGAYDTTIFECTDSQVRQALNHIQKEEQQHGEGIFNYMQQHGMYQPQ"
     gene            6028..6885
                     /locus_tag="VFC74_03475"
     CDS             6028..6885
                     /locus_tag="VFC74_03475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010966558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK24433.1"
                     /translation="MVVRILTDSTSYIDEQTKEALDINILPLHVSFQDESMKETEIEN
                     NLFYEMMAQKGIPVSSQPSIGELYAAMEDIVASGDDLFCVFLSSEMSGTYATACKVKE
                     MIVEEYPEAKIGVVDSRSNAMQLGFAAIVAARAAKEGKTLAEVKQAAEENIKRSRFLF
                     IPENFVYLRKGGRIGGAGALIGNLLKIVPILTVENGITTVAQKVRTKSKAITTMLEKT
                     WQDHLDYGLQEIVVHHINCYDQATDLANKLKEKLKINVGIANIGPVIGLHVGPGALGI
                     VYYTEKDMR"
     gene            complement(7103..7179)
                     /locus_tag="VFC74_03480"
     tRNA            complement(7103..7179)
                     /locus_tag="VFC74_03480"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7143..7145),aa:Pro,seq:cgg)
     gene            7481..8452
                     /locus_tag="VFC74_03485"
     CDS             7481..8452
                     /locus_tag="VFC74_03485"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZK24434.1"
                     /translation="MRVGIPRTLSYFTLYPLWKPFFETLGAEVIVSPESNKALLNDGV
                     KNTVSDACVPIKLFHGHIAALIGKVDILFMPRLVSLDGKSTLCPKFLGLPDMVRYSIS
                     GLPEIMESRLDMKKGKLELYRFLYRIGRKFTANRIRILKAIYRARYNFANYRKLLLKG
                     LTPVEAFYCLDSGQEPAWQTIASDIKLAVVGYPYTLYDNYISVGILQMLKRYGVQVVT
                     PEMVPGKEMKRMSKKLRKNLFWYYSNQVVWAAKYYLEDKNLAGIIHVTAFGCGPDSMT
                     NKLIELESKASKVPFISVTIDEHTGEAGVETRVEAFVDLIKRKKGMV"
     gene            8449..9525
                     /locus_tag="VFC74_03490"
     CDS             8449..9525
                     /locus_tag="VFC74_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HZK24435.1"
                     /translation="MKIAFPYMGTSHIAISYLLRELGHEVIYPPIPSKKTLSLGVQYS
                     PEFACLPFKILMGTYLEAVELGADTILASGGCGPCRAGYYGEVHRKILHDLGYPIEMI
                     VLEPPLIWPRDFFRQLHKLSKPRGWINLAKVFRVTWVKLKVLDQLESTLHQLRPLAIS
                     KQQITKVYEEGLELIDKAMSVQALNEAIEKALTRLRNVPLDQSSQPLKIGLIGEIYVV
                     LEPFANHDIEKLLGDMGVYVHRSIFLMNWTRDNAFGHGEKDVRAAAIPYLNELVGGHG
                     QNSIGEVVRYAEQGFDGVIHLAPFTCIPEIVSKSIMPAVSKDKGIPVLTLFLDEQTGE
                     AGIQTRLEAFLDLLKQKRIKQEAS"
     gene            9527..10501
                     /locus_tag="VFC74_03495"
     CDS             9527..10501
                     /locus_tag="VFC74_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008047 - enzyme activator activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase"
                     /protein_id="HZK24436.1"
                     /translation="MQAYLGVDVGSVSTNLVVIDEDCQVLVDIYLRTSGQPIKVVQEG
                     MRQVAEDVKGKSIEICGVGTTGSARTLTGVIVGADTVKNEITAHAVAASYLVPDVQTV
                     LEIGGQDSKIILLREGIVVDFAMNTVCAAGTGSFLDHQAARLNIPIEEFGGLALQSTS
                     PVRIAGRCSVFAESDMIHKQQMGHPTPDIVAGLCDALVRNYLNNVGKGKEILAPVVFQ
                     GGVAANVGIRAAFEKELGLEVFIPEYFSVMGAIGAALLAKSRTEEKQSTNFRGFAISS
                     TDYQATSFECKGCPNCCEIVNISADGELLARWGGRCGKWDTLQQTKTG"
     gene            11005..11964
                     /gene="dusB"
                     /locus_tag="VFC74_03500"
     CDS             11005..11964
                     /gene="dusB"
                     /locus_tag="VFC74_03500"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004035640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HZK24437.1"
                     /translation="MRIGKVNLKNKVLLAPMAGVSDSAFRQIAVEMGCALVYTEMVSA
                     KGIICAPAQSLKLAHFTTAERPIAIQLFGSEAKVMAEGAAVCARLKPEIIDINLGCPV
                     KKVVSKGEGCALMRDPSRVFDLVQAVTRAVTPLPVTVKIRKGWDEDSINAVEVALAAQ
                     EAGAAAVAVHGRTRTQGYSGRADWAIIRAVKKKLTVPVIGNGDIWQPKDAARMLEQTG
                     CDAVMLARGVLGNPWLIQRTVHYLREGTLLPEPDVAEKVVLALRHLEMQVQIKGEAIG
                     VREMRKHFAWYLKGVRGAASFRNQIMRVETKGEIEQILRSVMV"
     gene            12079..12618
                     /locus_tag="VFC74_03505"
     CDS             12079..12618
                     /locus_tag="VFC74_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK24438.1"
                     /translation="MEFIRIGEKLISAHKINDTVQRILHLRSEGLSQQEVAAKLQIDR
                     TFISRLESIGNVRRGGRIGLVAFPVQNKEELIKLADRYGIENRLILTNQERWQLVAKK
                     NGLDFLNKMMEIIEQLRQCETVLVFCSKKWNRIARALLDSEVFTTEIGSSPISEDIYI
                     NPTQIESILASFVSEGEEK"
     gene            12618..13517
                     /locus_tag="VFC74_03510"
     CDS             12618..13517
                     /locus_tag="VFC74_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008909571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate 5-dehydrogenase"
                     /protein_id="HZK24439.1"
                     /translation="MKRVVSISLGSSTRDHAADLELLGVPCRVERIGTDGDMKKMVAM
                     IKELDGQVDAFGLGGMDLYVYAGAHRYTLRDAKKVVKAAERSPIVDGSGLKNTLERKV
                     IQYLQRETDVLAGAPHVLMMSGVDRFGMTQALDAAGCRLTCGDLVFTIGIPLPLRSLR
                     SLEKLARLLAPLISQLPFSMLYPTGKKQDENKPKASHLFAEAEIIAGDFLFIRRYMPE
                     RLPGKVIITNTTTAADVELLKERGVKTLVTTTPVLNGRSFGTNVMEGLLVALSNKGRE
                     LTASEYEKLLLDVNFAPRITQLN"
     gene            13527..14600
                     /locus_tag="VFC74_03515"
     CDS             13527..14600
                     /locus_tag="VFC74_03515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HZK24440.1"
                     /translation="MGDFAFIIHPIELDDVFRKFKFMKKWPTPLVEGLLTMAPPFKVS
                     EITGVRSDHAETSGYFVTCPLTSKQMVTLPEKKVLKKIIQTGKVAQKLGAKVVGLGAM
                     TAVVGDAGITIAKNLDIAVTTGNSYTVATALQGTKKAADLMGIDLSEAEILVVGATGS
                     IGSVCARILARDARFLTLAARDREKLDYIAARIMRETGLAAKVSTDIKRSVQRADIII
                     TVTGAAEAVIDVMDLKPGAVVCDVARPRDVSKRTAALRDDVLVIEGGVVDVPGEVDFG
                     FNFGFPPRTSYACMAETMLLALEKRFEPFTLGRDLSVEQVDEITCIAEKHGFGISGFR
                     SFERAVTNEEIAVVRKNAARNQL"
BASE COUNT         4136 a         2812 c         3600 g         4242 t
ORIGIN      
        1 cctctttact acgttgattt atttcatata gtttttcgtt cacagaaata gaatttctcc
       61 gaagattctc ctgcgccagc tccagcatat gacggagctg ggctaaagaa gcttcttttt
      121 tgctgagttc atattctaaa cgtttttttt cagccatcaa gcgacgtgca tgtaaaaata
      181 gaggactttc ttcttcttct ttccgggatg tttgtacttg tttgtcttct tgcagtgttt
      241 taaccacatt tactaaaagt gatgtctcct cactttgctt aatcaagcgg atttgctgac
      301 ttgttgtggg ggcgaaacga gcgggaatta gtatttccgc agatgagctg cagctgattt
      361 ctgtttcttc gagtgggtta tagtaatgca ctagcttatc ttcatccatt aaccagagct
      421 ggggctgcgg ataagcctca ataaacgaca tcagctgcac agaaaactgc agaggtaaag
      481 tgactttgtg ctgccagccg ccactcggcc agcctcccgc taatctggcc tgataatgca
      541 gcaaatcatc ttcctgccag gcaagatgca ggttttggaa tgagtcaaat aaaaaggctg
      601 gtcttgttat gagggtgtag ttagtggcta aataaaatgg tttagttgga cgatatgttt
      661 tgggttccag cgattgatag taaagcattt tttcagcacg atcaaaaagt aatagatgaa
      721 gataagcagc ctgatcgata gccatagcta acggtgctga ttgctgacca ccagggatgt
      781 cataagtttg ccactgcatg gtttcgttta agagagcaaa tgttaggccg tctttgctct
      841 ctgcacaata atagcctaac cccgtactgt cgaaagctaa agtaaaagca gagggggcag
      901 cattggtgaa aaaggggagc tgctctattt catcatcggc gagtagaaaa gataagagct
      961 cattttgttg attggctgtc agtatgtaca gttgttcact gttattgact gcagcacaaa
     1021 accgcgcaca gttaaccagc acttctgttc gtttaatatt ttcgtctgct aaattaaaac
     1081 gagtgctgct taatacacca tcctgaccaa gctccagcaa ataaatatta gtttgactgt
     1141 ggaaaatgaa tggcatggta agtagttgct tcatttttgt tcctcccttc ctataatgat
     1201 atatatcact ttgcctgcac taagatgaca ggaaatgttt tagctcactg cacactctat
     1261 cgccgggtcg catatattag catagaactc ggtcgtaaaa ctgtaattga tcttacatac
     1321 ctacatgaaa ggaggttgca gaaaaacgtg aaaaaatata aaggcggatg tggtgggcat
     1381 ggtggtgggc atggcgatga ccaagtcgca gatgtacaac tgggccaatg tgaccccatt
     1441 tatggctgcc cgccaccaac ggaagttgtt tgtctgttag tagataaggt ttatgatgag
     1501 tgtaaaaatg ttcaggttga tgaagttgag ttcacttacg aagcctgccc ggaaaacccc
     1561 attgtagatg ttctttgtta caaagttgaa attattgatg gaccgtgttg tgaagttgtg
     1621 tctagcggcc gtgttcgtgt tactgtaagc tacaaagtta aaattaagtt gatctttgag
     1681 gatggctcca cacaaaagtt atgtgaagaa aagacggtta ttaagacatt taatgtgccg
     1741 cgtgcaggag aaccaggatt tttccttcac tgtgaggtac catttcttga gtgtttgcag
     1801 gcatttgtcg agagtgaaga attggaatac ggtgcattac gtacgaccat tattgcctgc
     1861 attggtaaat acaccttgtt gaagctttta gcaactgttc aaatagctgt accctcttat
     1921 ggcttttgcc cagagccccc cgattgtgat gaagttcttg gggaatgtcc agactttaat
     1981 ccagagtggc caccctaccc cctacaggat aggtaaacac cagaaaacgc cggtaacacc
     2041 ggcgtttttt gggttgttgc aggtttgctt gctcaataga tggaggtttt tagcaaaatc
     2101 ttaataattt gtctctaaaa cttaattttt agagcgtttt tttttagtat aattaaagtg
     2161 tagctgaaaa cactggtaca agtacggggt gtgaagcgaa tcgcaactat ctacttttcg
     2221 tgctatgtag aaggggaatg attagaattg tcgaacgtaa tatatagtaa tagggtattt
     2281 taagcaaagt taccagacac tgtttcgtgc agggaagatt tctatctgct aaaaaaggac
     2341 atactgatag tagtataatc atggtattga gagcacgatc tgatgctagg agggactttt
     2401 gtgagaattg gcatgtttac tgacagttat aagccataca ccagtggagt agtgcggtcc
     2461 atcgaaacaa ctacggctaa actgacagaa ctgggccatg aagtttatat ttttgcccca
     2521 aattatccta attgtgaaga agaagaaggc gtttttcgtt ttgcgtctgt gccgacacca
     2581 acttatccag acttttctat tgccttaccg ttttcgctta atttaagtgc cactgtgaaa
     2641 cgtctagatc ttgatgtaat tcatgtgcac tcaccgtttt ctatgggttt actaggctct
     2701 cgctgcgcca aacgtttcga tattcccttg gtttttacct atcatacgat gtatgatcag
     2761 tatgtgcatt atttgccttt tgcccaaggt ttttcgaaaa aggttgtttt gaagttatcc
     2821 tgtaattttt gtaaccgttg cgacctagtg attgcaccaa ctaatatcgt gcgtcagatt
     2881 attgctccaa atgtagttac gaaagtgaaa gctatcccaa ctggtattga gatcagtgag
     2941 tttgcgacgc tagaccggca gtggctacgt gagcagtacc agattcctca cgagaaaaaa
     3001 attctgctgc atctggggcg tttaggtaaa gagaaaaatg ttggtttctt gctgcagtct
     3061 taccggatga ttcgacaaac tcaaccagaa actgtcttag taattgtggg ggacggccct
     3121 gatcgggaat cattggagaa ccaagtaaat gaaatgggac tgtcacaaac agttattttc
     3181 acaggaacta tgcccagaga gcaggtagtg aacgcctatg ctggtgccga tttgtttatc
     3241 tttgcctcaa ctactgagac acaaggtcta gtgttaggtg aagccaaagc agccgctttg
     3301 ccttcagttg ccgtaaaagc attaggtgct gcagagatgg ttaaagacgg tatcgatggc
     3361 tttttaacca cactatcatt agatgacttt actgcccgtg tccagcagtt attgcaggat
     3421 gaacaattac gacaggcgat ggccgcaaga tgtgtggagg cggccgaaga aatttcctcc
     3481 atcaatatgg ctaaaaagtt agttactgct tatgagggag ctattaatga caagcaatgt
     3541 cgagatgtcg gctagtaaaa taaaacaccc cagcagggtg ttttatttta ccgctaatat
     3601 ttagctcact ttggctgtgg caaaactgct aatgtaagta gtatcacaag ctttagctta
     3661 tgtcttgatc tataggttat caagtagatc ttttattttg gtggcggtgt ggccgcttat
     3721 ttctgcaaat tcaaaaaaag ctttgcggac ttcgtcatta tctgtttgtg ttgcgaagcg
     3781 ctgataatcc cgtacctgtt cttgctcatt cagcatggct ctttctaaaa acgatttaac
     3841 ttccatgtca tcacctcccg taatataatg ttgccaggga aaaataaatt atgtatgaga
     3901 agttgtcaca cgattgtttt aaactttgtt aatattagag gaagttttca tttgttagcg
     3961 tattctgtac aaggaggtga tctgcttgaa aaaactatat cgttcacgtg accaacagat
     4021 gttgggcggc gtttgtatgg gtattgcccg ctattttaat gtagatgtga ctttaattcg
     4081 tttactttgg gtcatcggag gtctagctgg tggcggtatt ggcttacccg cgtacatcat
     4141 tgcttggatt atcattcctg aggaacctgg cattggtgaa gctattgata tttcgtcatc
     4201 cgaaggtgag gcgccgaaca ttgattcgcg taccgttggt ttggttgtga ttgctgtggg
     4261 cgtgttcctg ctgatacgcc agtttctccc gctctctatt ttccgctatt atttgtggcc
     4321 tctggccttc attggcgtcg gacttctact tgtttggggt ggtagaaagt gaaagctgac
     4381 cgcattgtct taggtctggc gattgtttta attggtctaa tgtggctgct cgttaacttg
     4441 ggtgttgtac ctgctgtgac agtacgtcaa ctttggcgtt actggcccct tttactaatt
     4501 ctctgggggg ttctcttgct cttaggtaag gataatagcg gggtaacagg ttttctggta
     4561 gttctacttg tgctggcatt agtgggggga ggttttttca cacattttac tttttcagga
     4621 cctaataatg cagaaacatt tgaaaccacg attagtaaca gcgataatgt gcaggcagtt
     4681 aaaatgcagt taattcagca tgcgggtgaa cttaacctgg catcacacca gggtgacgag
     4741 ctggcagagc ttaaattgca ggcttctgca cagccaagtg tgtcgcataa gcaggctgaa
     4801 gaaatagctg aaattatcat tgaggatcaa tctaataact taaattttga caaaaaggtt
     4861 tcacggtggg agctttcgct ggcggaaaag ctgcctttgc agctttcttt gcaaacagga
     4921 gccactaaag ctaacttgga tttttcgcat ttgctgctca aaaaactaga gatcgaggct
     4981 ggagccggtg atattgacct caagctaggc caatctgact gtgagattac tatcgaaagt
     5041 ggagccagta atattacaat ccggataccc gaagctgtcg gggtacgtct gcgtactagc
     5101 ggtgctttaa tgagcgtgga tagtgatgag ggccgaatga ttagtgtagg agaccgtagc
     5161 tacgatagta aaaacctaga aacaaaagaa gccattgccg atattcatat tgccgcagct
     5221 gctggcaatg tgacgcttcg tcatgactaa cttaacttgg ccagcacagc caagcagctc
     5281 cttttggcct actgtttacc cctggaaatc gcagctgctg cagggtcatc gggacgtgcc
     5341 atcgcgatga taagatgaat aaaacaactc ttattgcaaa aactgcaagc aggaggtgtt
     5401 ttataatgca gttaacccag aaagaaagaa tgttgttaga ggatcaaaaa aatcaggaag
     5461 aactatgcat taaaaagtat acggaagcag cacagaaggc aaaggatcca caactccagc
     5521 aattgtttac tacctatgcc gatcaggagc gtcaacatta tcagactgtt gaacaaatta
     5581 tgcagggcca ggttcccagt acacagccag gttcgcaagc aggacagcag agtggccaac
     5641 agcagtccgg gcagaatatg cctggaggca ccacagcact ggccagccaa tcggatgctg
     5701 atctttgtaa cgatatgtta tcaacagaaa aatatgtctc tggtgcttat gacaccacaa
     5761 tctttgaatg tactgactcc caagtcagac aagctttaaa ccatattcaa aaagaagaac
     5821 agcagcatgg tgaaggaatt tttaattaca tgcagcaaca tgggatgtac caacctcaat
     5881 aataaagcaa ggcctaaggc tgcggcaatt atgaaacagt gtttcttttt gccgcagcct
     5941 tcttttataa tatctaaacc ctgctgtcta tgatataatg ctaacgtcat gccgacatgt
     6001 cttttttaaa ttgggagtgg ataaattgtg gtggtaagaa tattgaccga tagtacgagc
     6061 tatattgatg aacagactaa agaagcgctg gatattaata tactgccttt acatgtttcc
     6121 tttcaagacg aaagtatgaa ggaaacggag atagaaaaca atttattcta tgaaatgatg
     6181 gcccagaagg gtatccccgt atcctcccag ccctccatag gagaattata cgcggctatg
     6241 gaagatattg ttgcctctgg agatgatctt ttttgtgtct ttttatcgtc agaaatgagt
     6301 ggtacctatg ctaccgcctg taaagtaaaa gagatgattg tagaagagta tccggaagct
     6361 aaaattgggg ttgttgattc ccgttccaat gcgatgcaat taggatttgc cgctattgta
     6421 gctgccagag cggctaaaga gggtaaaaca ttagctgaag taaagcaggc agccgaggaa
     6481 aatattaaaa gaagtaggtt tctgtttata cccgaaaact ttgtttattt gagaaaaggc
     6541 gggcgcatcg gtggagcggg tgcattaatc ggaaatctac tgaaaatagt tcctatttta
     6601 acggtggaaa acggcataac aacagtagcc caaaaagtca gaactaagag taaggccatt
     6661 acaactatgc tggaaaaaac atggcaagat cacctggatt acggcctgca ggaaattgtt
     6721 gtgcatcaca ttaattgcta tgatcaagca acagatttag ccaataagtt aaaagaaaaa
     6781 ctaaagatca atgtaggcat cgcgaatatt ggtcctgtca ttggacttca tgttgggccg
     6841 ggggcactgg gtatcgttta ttatacggaa aaagatatgc gctaaagcct ctcttttttt
     6901 ctgcacatat taacgggtta atactatcct gaagggaaaa attgtcgctt aaaaacgatg
     6961 ctcgccagct atttttaacc taaaaaaaca agctgcggaa tagggcagct tgcctcacgc
     7021 aggcgtcaga tgatagcctg tgtcttgtga acaaaaaaac cacgggaagc ttaaacgctc
     7081 ccgtggtttc tacgttcatg tatggtcgga gcgacaggat ttgaacctgc gacctcttgg
     7141 tcccgaacca agcgcgctac caagctgcgc tacgccccga cgcgaacttt attgtagcat
     7201 aagataagct aaattgcaat agtttaggag gagaccggca cacggtgctt tccgtttggc
     7261 cccagttgag ttaaagccta agatttttct ctaaacctcc tgaaggttgg gttattattt
     7321 actgtttttt atgctatata ttagtagatt acttttaccc gccgctgcat tgttaagtgt
     7381 ttgttaagct tgacctagca ggaaaacatc ctcctattct cgaataaaaa accatgatac
     7441 actattcaat aaatcccaaa ataagaaagc aggtggagca ttgcgggtcg gaattcctag
     7501 aacattgtcg tactttacac tctatccatt gtggaaacct ttttttgaaa cacttggtgc
     7561 tgaagtaatt gtctcccccg aatctaacaa agcattacta aatgatggtg ttaagaatac
     7621 tgtttctgat gcctgtgtgc caattaaact ttttcacggt catatcgctg ccctgatcgg
     7681 caaagtagat attcttttta tgccaaggtt agtttcactg gacggcaaaa gtactttatg
     7741 tccaaaattt ttaggtttac cggatatggt gagatactcc atcagtgggc ttcctgagat
     7801 tatggaaagt cgactggata tgaaaaaagg gaagctggaa ctttatcggt ttttataccg
     7861 cattggacgc aagtttacag ctaataggat cagaatttta aaggctattt atcgtgccag
     7921 gtataatttt gccaattaca ggaaattatt attaaaaggc ttaactccgg tagaagcatt
     7981 ttactgtctg gatagtggac aagagccggc gtggcaaact atagccagtg atattaagct
     8041 tgccgtagtt gggtatcctt atacccttta tgacaattat atcagtgtag gcatcttgca
     8101 gatgttaaag cggtatgggg ttcaggtcgt tactccagag atggttcctg gaaaagaaat
     8161 gaagcgcatg agcaaaaagt tacgtaaaaa cctattttgg tactatagca atcaggttgt
     8221 gtgggctgcc aagtattatt tagaggataa aaatttagct ggcatcatcc atgtcactgc
     8281 tttcggttgt ggaccggatt ccatgacgaa caaactaatt gagctggaaa gcaaggctag
     8341 taaagtacca tttatctcgg tgactattga tgaacataca ggagaagctg gtgtagagac
     8401 aagagtggag gctttcgtag atttaatcaa gagaaagaag gggatggtat gaaaattgct
     8461 tttccctata tggggacttc acatattgcc attagctact tacttagaga actagggcat
     8521 gaggttattt atcctccgat accatcaaag aaaaccctct ctttaggtgt ccaatattca
     8581 ccagagtttg cctgcttacc gttcaaaatc ctgatgggta cctatttgga agcggtagag
     8641 ttgggtgcag atacaatttt ggcttcggga ggctgtggcc cttgccgcgc aggctactat
     8701 ggcgaggtac accgcaagat tctgcatgat ttagggtatc caattgagat gattgtctta
     8761 gaaccgccac ttatttggcc gcgtgatttt tttcgccagc tgcataagct ttctaaaccc
     8821 agaggctgga taaacttggc caaagtgttt agggtgacgt gggttaagct caaagtactg
     8881 gatcaactag aaagtaccct acatcaactt agacctttag ccatttctaa acaacaaata
     8941 acaaaagtct atgaagaggg cctggaactg attgataagg ctatgtctgt ccaagcatta
     9001 aacgaagcga tagaaaaggc gcttactaga ttacgcaatg ttccgctgga ccagagctct
     9061 caaccgctta aaataggcct aattggtgaa atttatgttg tactagaacc ttttgctaat
     9121 cacgatattg aaaaattact tggggatatg ggagtctatg tgcatcgctc tatttttctt
     9181 atgaattgga ctcgcgataa tgcgtttggt catggggaaa aagatgtgcg agcggcagca
     9241 attccctatt taaacgaatt agtgggtggt catgggcaaa atagtattgg tgaagtagtt
     9301 cgttacgcgg agcaaggatt tgatggtgtt attcacctag caccttttac ctgtattccc
     9361 gagattgtct caaaaagtat tatgcctgct gtcagcaagg ataaaggaat tccggtctta
     9421 acactatttt tagatgagca gactggggaa gcgggtattc aaacccgact cgaggcattt
     9481 ttagatttgc tgaagcagaa acgtatcaag caggaggcga gttagtttgc aagcatacct
     9541 tggggttgac gtcggttcgg tcagtacgaa tttagtcgtc attgacgagg attgtcaggt
     9601 attggttgat atatacttac gcacctctgg tcagccaatt aaggttgtgc aagagggtat
     9661 gcgtcaagta gcagaagatg taaaaggtaa aagtattgaa atctgcggtg tcggcacaac
     9721 aggaagtgcc cgtaccctaa cgggtgtgat tgtcggtgca gacacggtca aaaatgagat
     9781 tacggcgcat gcggtagcag cgtcttatct agtgccggat gtgcagacag ttttagaaat
     9841 tggcggacaa gattcaaaga taatcttgct ccgagaggga attgttgttg attttgcgat
     9901 gaatacagtt tgtgctgccg gaactggttc gtttttagac catcaagcag ctaggctcaa
     9961 tattcctatt gaggagtttg gtggcctagc tttgcaaagc acgagcccag ttcgtattgc
    10021 aggcagatgt tctgtctttg cggaatcgga tatgattcac aaacaacaaa tgggacatcc
    10081 aacacctgat attgtggctg gtctctgtga tgcactagtc cgtaactatc tgaataatgt
    10141 cggcaaaggt aaagaaattc ttgctccggt ggtttttcag ggtggtgtag ctgccaatgt
    10201 tggtatccgc gcagcctttg aaaaagaatt aggtttggaa gtttttattc ctgaatattt
    10261 tagtgtaatg ggagctattg gtgctgcttt gctcgcgaag tccagaacag aggaaaagca
    10321 aagtactaac tttagaggct ttgcaatttc ctccactgat tatcaggcca caagttttga
    10381 atgtaaaggt tgtcctaatt gctgtgagat tgtcaatatc tcggcagatg gcgagctgct
    10441 ggcacgctgg ggtggtcgtt gcggaaagtg ggatacacta cagcaaacaa aaacaggtta
    10501 gaaagacact gcggtgtctt tttttttctc tcaatcaaga caggttttgc caatgcaggc
    10561 aggaattaaa ggttttctgt cgaacaatag ttgcaagaag tgaaaagtat tttataaacc
    10621 acaatttatt acatgggagt gaaaagaatg tcatccgtat tattgggacg aaggctacgg
    10681 gctttacgtc gcctaaaaag gcttacgcaa ctagaattgg cctcagaagt aggaatttct
    10741 gtttcgatgc tttcaacaat tgaacgaggt gcaaaatatc cccgtgtgga tctaataagg
    10801 aagtttgccc gtactctaaa tgtgccatta gaagaatttt ttgtgcttcc tgaatacgtt
    10861 gaaaagcgcc cctgaggcgc ttttttatac tgacaagaaa attgtattgc ttgcatttta
    10921 aagtgaaagt gcaagctttt ttattttata attgcctaaa gcagatatac tagagataaa
    10981 gatgattttg gagggagcgc agtaatgcga ataggaaaag tgaacttaaa gaataaagtg
    11041 ttacttgcgc cgatggctgg ggtgtcggat agtgcttttc ggcaaattgc cgttgagatg
    11101 ggttgtgcgc tagtttatac agaaatggta agcgctaaag gtatcatctg cgcgcccgca
    11161 caatcattaa agcttgctca ctttacaaca gctgaacgac ctattgccat acaattgttt
    11221 ggtagtgagg ctaaggtgat ggccgagggg gcagctgttt gcgcgcgctt aaaacccgaa
    11281 ataattgata taaatctggg ctgcccagta aaaaaagtgg taagtaaagg tgaggggtgc
    11341 gcgttaatgc gtgaccccag cagagtcttt gacctcgtac aagcagttac acgggcggtg
    11401 actccgctgc cggtgacggt taaaatccgc aaaggctggg atgaggactc tattaatgca
    11461 gtggaagtgg ctttagctgc ccaggaggcg ggtgctgcgg ccgtagctgt gcatgggcgt
    11521 acgcggacac aaggttatag tggccgcgcg gactgggcaa ttatccgtgc tgttaaaaag
    11581 aaactcacag ttcccgtaat tggcaatggc gatatctggc agcctaaaga tgccgctagg
    11641 atgctggagc aaacaggctg cgatgctgtg atgttagcac gtggggtctt gggtaatccc
    11701 tggctaattc agcgcacagt tcattattta agggaaggca cattactgcc cgagccagat
    11761 gtggctgaga aggtggtttt agctctacgc catttagaaa tgcaggtaca gataaaaggt
    11821 gaagctattg gtgttaggga aatgcggaaa cattttgctt ggtaccttaa aggtgtccgc
    11881 ggcgcagctt cttttcgcaa ccagatcatg cgggtcgaaa ctaaaggcga aatagaacag
    11941 attttacgtt cggtaatggt gtagctacag ttgtacaagt taatttggtt tcgctgcgga
    12001 aactcttgca tcctggaaaa ctacgtggta aaatcgaatg tacacaattc tgtgcacaat
    12061 gtagaaagag gagctatgat ggaatttata cgcataggtg agaagttaat tagtgctcat
    12121 aaaatcaacg atacggttca gcgcattctg catttacgca gcgaaggcct ctcgcagcag
    12181 gaagtggcgg caaagctgca aattgacaga acttttattt cgcgacttga atcgatcggt
    12241 aatgtgcgcc gtggcggccg cattgggtta gtggcttttc cggtgcaaaa taaagaggaa
    12301 ctaataaaac ttgccgatcg ctatggtatc gaaaacaggt tgattcttac taatcaggaa
    12361 cgctggcagc tggtagctaa aaaaaatggc cttgatttcc tgaacaaaat gatggaaatt
    12421 atcgagcagc tgcgccaatg cgaaacagtc cttgtctttt gctccaaaaa atggaatcga
    12481 attgctaggg cgctgcttga tagtgaggta tttaccacag agattggcag ttcacctatc
    12541 tctgaggata tatatatcaa tcctacacag atagaaagca tccttgcttc atttgtcagt
    12601 gaaggggagg aaaaatagtg aaacgagtag ttagtataag tttgggttcc tcgacacgtg
    12661 atcatgctgc agatctcgaa ttgttaggtg tgccctgccg cgtggagcgt atcggaacag
    12721 atggcgatat gaaaaaaatg gtggccatga tcaaggaatt ggatggccag gttgacgcgt
    12781 ttggtctggg aggtatggac ctctacgttt acgccggagc acatcgctat acactgcgcg
    12841 atgcaaaaaa agtggtaaaa gctgctgaac gtagtccgat tgtggatgga tcaggattaa
    12901 aaaatacttt agagcgaaaa gttatccagt atttacagcg agagacagat gtgctagccg
    12961 gagcccctca tgttttaatg atgtctggtg ttgaccgctt tggcatgacg caagctcttg
    13021 atgccgcagg atgtcgtcta acttgcggag atttagtttt tacgatcggc attcccctgc
    13081 cgcttcgttc attgcgttct ctagaaaaac ttgctcgttt attggcccct ctgattagcc
    13141 agcttccttt ttctatgctt tatccgactg gcaaaaagca ggacgaaaat aaacccaagg
    13201 cgagtcacct ttttgccgaa gcagagatca ttgcggggga ttttcttttt atccgtcgtt
    13261 acatgccaga aaggctgccg ggaaaagtta ttattaccaa taccaccact gccgcagatg
    13321 tagagctgct taaggagcgg ggtgtaaaaa cattagtgac caccactcca gttttaaacg
    13381 gccgttcttt tggtacgaat gtgatggaag gtcttttggt ggcactgtca aataaagggc
    13441 gggagttaac cgccagtgag tatgagaaat tgttacttga tgttaatttt gcgccacgaa
    13501 ttacgcagtt aaactaggag ggctttatgg gcgactttgc atttattatt caccccatcg
    13561 aattagatga tgtttttcgt aagtttaagt tcatgaagaa gtggcctacg ccgttggtcg
    13621 aaggattatt aacaatggca ccaccgttta aggtctcaga aattactggt gtgcgctctg
    13681 atcatgccga aacatccggc tattttgtga cttgcccctt gacctctaaa caaatggtga
    13741 cattgccaga aaaaaaagtg cttaagaaga taatccaaac tggtaaggtt gcccaaaaac
    13801 taggcgcgaa ggttgtaggg cttggggcaa tgactgctgt tgttggcgat gcagggatca
    13861 caattgctaa aaacctagat attgccgtga ccacaggcaa tagttatacg gtagccactg
    13921 ctttacaagg gactaaaaag gctgctgact taatgggaat tgatttgtcg gaggcagaaa
    13981 tcctggtagt tggagccacc ggctctatcg gcagcgtttg tgcgcgaatt ttggcaagag
    14041 atgctcgttt tttaacactt gctgctcggg atagagaaaa attagattat atcgcagcac
    14101 gtattatgcg ggaaaccggc ttggcggcca aggtgtcaac agatattaag cgctctgtac
    14161 agcgtgctga tattattatt actgtcaccg gtgcggcgga ggcggtaatt gatgtcatgg
    14221 atctcaaacc cggtgctgtt gtttgtgacg tagcacgtcc tcgcgatgtt tctaaacgaa
    14281 cagcagccct aagagatgac gtcttagtca tagagggcgg tgttgttgac gtgcctgggg
    14341 aagtggactt tggtttcaat tttggttttc ctccacgcac ttcatatgcc tgcatggcag
    14401 aaaccatgct gttggcttta gaaaaaagat ttgaaccatt tactttaggt cgtgatttat
    14461 cagtggagca agtggatgaa ataacctgca ttgctgagaa acacggcttt ggcatttcag
    14521 gtttccgtag ttttgaacgt gctgttacaa atgaagagat tgcagttgta cgtaaaaatg
    14581 ctgctcgcaa ccaactataa aaaataggcc gtatgtgcgg cctatttttt ggctttagct
    14641 attgttgcta gttagtgtaa acttactata gggcttttgg aggaaaacac ccccaatttg
    14701 tagaaaaaag acttcaccgt cccggaagga caagtaacta aaaatagtca ctcgaggtga
    14761 agtcttatga aaatagttca acacatgctt
//
LOCUS       DASRMS010000085        10016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_624026, whole genome shotgun sequence.
ACCESSION   DASRMS010000085 DASRMS010000000
VERSION     DASRMS010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 10016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10016
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_624026"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFC74_03520"
     CDS             complement(<1..579)
                     /locus_tag="VFC74_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK24441.1"
                     /translation="MAKQKFERTKTHVNIGTIGHVDHGKTTLTAAITSCLSTAGFAQK
                     QAFDQIDKAPEERERGITISTSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFMNKADQVDDDELLELVEMEVRDLLTE
                     YEFPGDDTPVVIGSALKALEHGCGSRDCPDCKV"
     gene            complement(675..750)
                     /locus_tag="VFC74_03525"
     tRNA            complement(675..750)
                     /locus_tag="VFC74_03525"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(715..717),aa:Met,seq:cat)
     gene            complement(759..834)
                     /locus_tag="VFC74_03530"
     tRNA            complement(759..834)
                     /locus_tag="VFC74_03530"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(799..801),aa:Thr,seq:ggt)
     gene            complement(849..924)
                     /locus_tag="VFC74_03535"
     tRNA            complement(849..924)
                     /locus_tag="VFC74_03535"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(889..891),aa:Met,seq:cat)
     gene            complement(937..1010)
                     /locus_tag="VFC74_03540"
     tRNA            complement(937..1010)
                     /locus_tag="VFC74_03540"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(976..978),aa:Gly,seq:ccc)
     gene            complement(1202..1867)
                     /gene="sigH"
                     /locus_tag="VFC74_03545"
     CDS             complement(1202..1867)
                     /gene="sigH"
                     /locus_tag="VFC74_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigH"
                     /protein_id="HZK24442.1"
                     /translation="MQVSVTAQEVRGLANAYKNYEVQTDEEVAMQAKSGCEVAQEYLI
                     NKYRNFVKAKARSYFLIGADREDIIQEGMIGLYKAIRDFKEDKLSSFRAFAELCITRQ
                     IITAIKTATRQKHIPLNSYVSLNKPIYDEDSDRTLLDILSGVKITDPEELMINREEYS
                     NIEIKMNEILSDLEWKVLTFYLEGKSYQEIAVELKRHVKSIDNALQRVKRKLERYLEL
                     REN"
     gene            complement(2001..2738)
                     /gene="rlmB"
                     /locus_tag="VFC74_03550"
     CDS             complement(2001..2738)
                     /gene="rlmB"
                     /locus_tag="VFC74_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HZK24443.1"
                     /translation="MEEKRMIEGRRPVMEALRAGSKITRILLQKGTHGGPLTEILALA
                     QTKEIPVEYWDKQALDKKAVTRNHQGILAETPPFAYTPFEQLLVKKNDEHPFLVILDH
                     LQDPHNLGALIRTAYAAGCDGLVIPERRAAQMTPAAVRTAAGAAEYLPVARVVNIARC
                     LEECKQAGLWLYGADMDGKSLYTKGDYQGPMALVIGSEGAGLSRLTKEKCDFLVRLPM
                     QGAVASLNASVAGALLLYEVYRQRQGF"
     gene            complement(2747..3433)
                     /gene="thyX"
                     /locus_tag="VFC74_03555"
     CDS             complement(2747..3433)
                     /gene="thyX"
                     /locus_tag="VFC74_03555"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HZK24444.1"
                     /translation="MKVTLLSHTPEPEKIVAAAARLCYSPDSVDVLLDNLTEEKTAQF
                     LDKLVEMGHLSPIEHVTFTFAVEGVSRTLSHQMVRHRIGSYSQKSQRYVSEDAFEYIV
                     PPSVAANEEALEIFTAQMENIRQSYQTLRQLVHQEDARYVLPNACETKFVFSMNARSL
                     HNFFKLRCCNRAQWEIRALAEAIYREVKKVAPTLFAYAGPSCVSEGACPEGKMTCGEI
                     TAVREKYRTM"
     gene            complement(3430..3813)
                     /locus_tag="VFC74_03560"
     CDS             complement(3430..3813)
                     /locus_tag="VFC74_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011641729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III domain-containing protein"
                     /protein_id="HZK24445.1"
                     /translation="MKNAEAYSPLLLAYIGDAVFELYVRQKLVCQQALPMQQMHRKVT
                     RIVRAAGQDAALRQLNTYLSAEEAAVVRRGRNTKSHVPKNAEMSVYRRATGFEALLGW
                     LYLSRQTERLYELLKLLKIGEEENV"
     gene            complement(3810..5216)
                     /gene="cysS"
                     /locus_tag="VFC74_03565"
     CDS             complement(3810..5216)
                     /gene="cysS"
                     /locus_tag="VFC74_03565"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003367590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HZK24446.1"
                     /translation="MRVFNTVTRKKEDFVPVNGNKVGIYACGPTVYDYFHIGNARIFI
                     VFDVIRRYLQWRGYDVTFVQNFTDIDDKMIKRAAELGITVEELADRYIQAYFEDVQAL
                     GVRPADVHPRATEHIQEMIVLIEDLIAKGMAYNVDGNVYFHTPAFHSYGELSQQPLED
                     LVAGARVEVDERKRHPLDFVLWKKQKPGEPAWESPWGLGRPGWHIECSVMSMKYLGET
                     LDIHAGGPDLIFPHHENEKAQSEGATGRPFAKYWLHAGYLNINQQKMSKSLGNFMTVH
                     DLRQVVDPEVVRFFMLSAHYRNPINYAADLLQQAKSGLERLNNVVYNLRDVLNKLPAS
                     SEQSDDESSEKLAALAVYRERFVEAMDDDFNTADALAVLFELARETNTYLSQPSPARP
                     VVEKTLAFFAEVGEILGFFADKQHNDLDSKVNELIERRQAARQAKDWSTADAIRDQLQ
                     AMGVVLEDTPQGVRWRKK"
     gene            complement(5203..5868)
                     /gene="cysE"
                     /locus_tag="VFC74_03570"
     CDS             complement(5203..5868)
                     /gene="cysE"
                     /locus_tag="VFC74_03570"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HZK24447.1"
                     /translation="MFHIKRDVKAIFERDPAAKSFLEVLLCYPGLHALWLHRLAHALY
                     RLGLVLLPRLISQFSRFLTGIEIHPGAKIGEGFFIDHGTGVVIGETTEIGDNVTLYQG
                     VTLGGTGKEKGKRHPTIGHSVVIGTGAKVLGPIEIGDNAKIGAGSVVLHNVPEHTTVV
                     GVPGRVVIFEGERVPSVDLNHVDLPDPVAEMMRCLQQQIDDLRFKQEKLLRKGEDNDD
                     ARL"
     misc_binding    complement(5906..6112)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(6169..7632)
                     /gene="gltX"
                     /locus_tag="VFC74_03575"
     CDS             complement(6169..7632)
                     /gene="gltX"
                     /locus_tag="VFC74_03575"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HZK24448.1"
                     /translation="MAKVKVRFAPSPTGPLHIGGARSALFNWLFARHHGGTMVVRIED
                     TDLDRSTREYEKLILDSLRWLGIDWDEGIDVGGPNGPYRQQERLPLYQPFIDQLLASG
                     QAYACYCTEEELEAERQELMAQGKTPRYLGKCRDLSVQEQEEKCGEGRKPAIRFRVPE
                     GKLLVVNDLVRGRVEFESNGIGDFIIVKSDGLPTYNFAVVLDDAAMGITHILRGEEHL
                     SNTPRQLLIYEALGFELPEFAHISLILGEDRSKMSKRHGATSVVQYRDEGYLPQALVN
                     FLSLLGWSPEGEEEIYEIDEIARLFSLQRVSKSPAVFNLEKLKWMNAHYLKVLPVGEL
                     TKLCLPHLQKAGLVEADLTADKLAWLEQTVATIQEKLEYAAQAPQLLQVFFGETVSWD
                     ENDAEAQEVLALETSPAVLKALLAKVQTLYAWDVAQVRQVLKGIGKELGVKGKALYMA
                     VRVAVSGQSHGPDLNALLALLGPATVAKRLTATLTRL"
     gene            7770..9770
                     /locus_tag="VFC74_03580"
     CDS             7770..9770
                     /locus_tag="VFC74_03580"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HZK24449.1"
                     /translation="MKNFFNLYNHGFVRVAVAVPQVQVADPTFNVTEIIALAQQAAAQ
                     NAVLTVFPELGVTAYSNEDLFHQQALLETAEREVQRLLAETSELNTILIVGAPLLIDS
                     FLYNCALVLLRGCLLGIVPKSYLPNQREFYESRQFRPTQTAINNTVDYGQQTAIPFGS
                     DLKFKVANLPHFTFAVEIGEDLTVPIPPSSFAALAGATVIANPAASPLTVGKAASRHT
                     LLAAQSARCRAAYLYAAAGLGESTTDLAWDGHALIYENGQALAEAPRFSLSSTLTLSE
                     LDLAGLTQERLHDNTFADNSLLLQAQNKHYRTITCEIPIPSAKLMLTRTLPRFPYVPA
                     NKTALAQQCFEAMQIQVQGLVKRLQSTGITKIIIGVSGGLDSTLALLVAVKAIDLLGL
                     PRHNIRAYSMPGFATSHRTKANAWQLMQALGVYAEELDIRPSSQQMLADIGHPVTRGE
                     TRYDATYENVQAGQRTAILFRLANLHQGLVLGTGDLSELALGWLTYGVGDHMSHYNVN
                     ASVPKTLIRALLQWQAEAEEIAQKTAAIILDVLKTEISPELVPGTDDDNQPAQHTEDY
                     IGPYELQDFHIYYTLRYGFRPSKIAFLAYNAWGAKYSLEEIKHWLTIFLKRFFASSQF
                     KRSAMPNAPKVVSTSLSPRGDWRAPSDAVATAWLTELASWDL"
BASE COUNT         2617 a         2659 c         2061 g         2679 t
ORIGIN      
        1 tactttgcaa tcggggcagt cacggctgcc acagccatgc tccagagcct tgagcgcaga
       61 accaatcacc acaggagtat cgtcaccagg gaattcatat tccgttaaga ggtcacgtac
      121 ttccatttcc acaagctcta aaagctcatc atcgtctact tggtcggctt tgttcatgaa
      181 aacaacaatg tagggtaccc caacctgacg ggccagcagg atgtgctccc gtgtttgcgg
      241 catcgggcca tcagctgcgg aaacaactag gatcgcgccg tccatttgtg ccgcaccagt
      301 aatcatgttt ttaacatagt cggcgtggcc ggggcagtca acgtgtgcat agtgacgctt
      361 ttctgtttca tattccacgt gagaggtgga aatggtgata ccacgttccc tctcttcagg
      421 tgccttatcg atttgatcga aagcctgctt ttgggcgaag cccgcagtag agagacagga
      481 tgtgatagct gcagtaagtg tggttttacc gtggtcaacg tggccaatag taccaatatt
      541 aacgtgggtc tttgtccttt caaacttttg ctttgccatt gctttaacct ccctgaataa
      601 gtagatttta ccaatgtaaa taaaaaaata tacttggcca agtcagtaac ttgtccaaga
      661 atttatgtac agattggtgg cggcgaaggg atttgaaccc ctgacgcggc gggtatgaac
      721 cgcctgctct accatctgag ctacgccgcc atttagattg gagctcacga ccgggatcga
      781 accggtgacc tcatccttac caaggatgtg ctctaccgac tgagctacgt gagcaaagct
      841 aagcagattg gttgcggggg caggacttga acctgcgacc tccgggttat gagcccgacg
      901 agctgccaac tgctccaccc cgcgacatta cgctattgga gcgggtgatg ggaatcgaac
      961 ccacgtgacc agcttgggaa gctggagctc taccattaag ctacacccgc atatacccga
     1021 aatcagcttc ttgattatac aatacatcaa tattgatgtc aactagcatc ttttaccgat
     1081 ttagggcagt cccaataact catttttccc tgctgccagc agggaaaata acggcttatc
     1141 acaactaaat cccataaaaa cgggcgaaca gtgttcgccc cgacattgtt tcacttacaa
     1201 gttagttttc ccgtaactcc aagtagcgtt ccagttttct tttgacacgc tgtaaagcat
     1261 tgtcaataga tttcacgtgc cgttttaatt ccacggcaat ctcctggtag gacttacctt
     1321 ctaggtaaaa ggttaaaacc ttccattcca ggtcactgag aatttcattc attttaattt
     1381 ctatattact atattcttca cgattaatca ttaattcttc aggatccgta attttaaccc
     1441 ctgagaggat atccagtaat gtacgatcag aatcttcatc atagatcggt ttattaaggg
     1501 acacgtagga atttaacggt atatgttttt gccgtgttgc tgttttaatg gcggtaataa
     1561 tctgccgtgt aatacataac tctgcaaacg ccctaaatga cgaaagtttg tcctctttaa
     1621 agtcgcggat agccttatat aagccaatca ttccttcttg aatgatatct tcacgatcag
     1681 cgccaatcag aaaataggac ctagctttgg ctttaacaaa gttgcgatac ttattgatta
     1741 agtactcctg cgcaacctcg cagccacttt ttgcttgcat agccacttct tcgtcagttt
     1801 gcacttcata gtttttatag gcattagcca gaccacggac ttcctgcgcc gtcacactca
     1861 cctgcatcgc ctccacgact gtataatcgt taccccgcaa cctggaattc agctcagaac
     1921 tcataaagat attataacgt actgctagta aaagcgtcaa gagcttttcg acagctattc
     1981 atgcggcttg gcgcaaatag ctagaaacct tgccgttgtc tatatacttc gtagagtaat
     2041 aaggcacccg ctacggaagc atttagcgag gcgacagcac cctgcatcgg caatcgcacc
     2101 agaaaatcac atttttcttt tgtcagccgg cttaagcctg caccttcact accaatcacc
     2161 agcgccatag ggccttggta atcgcccttt gtatacaggc ttttaccatc catatcagcc
     2221 ccatagagcc acaaaccagc ttgtttgcat tcttccaggc agcgtgcgat gttaaccacg
     2281 cgggcaacag gtaaatattc ggcagcgccg gcggcagtac gcacagcagc aggcgtcatt
     2341 tgtgcggccc gtcgttccgg aattaccaga ccatcacagc cagccgcata agcagtgcgg
     2401 attaaagccc ccaagttatg cgggtcttgc aaatgatcaa gaatgactaa aaagggatgc
     2461 tcgtcgttct tctttaccag cagctgctca aaaggggtat aagcaaaagg tggcgtttcc
     2521 gccaaaatac cttggtgatt acgggttaca gcttttttgt ccaaagcctg tttatcccag
     2581 tattcaacgg ggatctcttt agtttgcgcc aaggccagaa tttctgtcag tgggccgcca
     2641 tgagtacctt tttgcagtag gattcgcgta atttttgacc cagctctcaa tgcttccatc
     2701 accggtcgac gtccttcaat cattcttttt tcttccatgt gttacctcac attgtgcgat
     2761 atttttctct gacggcagta atttcaccgc aggtcatttt accctccgga caggcgcctt
     2821 cactgacaca gctcgggccg gcgtaagcaa atagcgtcgg tgctaccttt tttacttccc
     2881 tgtagatagc ttcggctaaa gcacggattt cccactgggc acgattgcag cagcgcagtt
     2941 taaagaaatt atgcaggctg cgggcattca tagaaaaaac aaatttggtt tcacaggcat
     3001 taggtaaaac atatcttgca tcctcttgat gtaccagctg acgcaacgtc tgataagact
     3061 gacggatatt ttccatttgt gcggtaaaaa tttctaaagc ttcctcgtta gcggcaactg
     3121 aaggaggtac aatatattca aaagcatcct cagaaacata gcgctgcgac ttttgtgagt
     3181 aagaacctat gcgatggcgt accatttgat gtgacagggt ccgactaacg ccctctaccg
     3241 caaaggtaaa agtaacatgt tcaatgggag ataaatgtcc catttctact aacttgtcca
     3301 aaaactgtgc agttttttcc tctgttaaat tatctaagag cacatcaaca ctatcaggcg
     3361 aataacatag gcgagctgcg gcagccacga ttttttccgg ctccggtgta tgggacaata
     3421 aagtgacttt catacgttct cttcctcccc gatttttaaa agtttcagca attcataaag
     3481 acgttctgtt tgccgactta aatataacca acctaaaagc gcctcaaaac cagtggcacg
     3541 tcggtatacg gacatctcag cgtttttagg cacatggctt tttgtattgc gtccgcgacg
     3601 caccacagcg gcttcctctg ccgataagta tgtatttagc tgccgtaagg ctgcatcttg
     3661 ccccgcagca cggacaatac gtgtcacctt cctatgcatt tgctgcatgg gtaaagcttg
     3721 ctgacacact aatttttgcc gtacataaag ctcaaaaaca gcatcgccaa tataggctaa
     3781 cagcagcggt gagtaagctt ctgcattctt cattttttcc gccaacgtac accttgcggt
     3841 gtatcctcca agacaacccc cattgcttgc agttgatcac gaatagcgtc agccgtggac
     3901 caatctttcg cttgacgagc tgcctgccga cgctcgatca gctcatttac tttgctgtcc
     3961 aagtcattgt gctgcttgtc ggcaaagaac cctaaaattt cgcctacttc tgcaaagaag
     4021 gctaaagttt tttccactac cggtctggca ggtgacggtt ggctaagata ggtgttcgtt
     4081 tcacgtgcta actcaaaaag cacggccaag gcatccgctg tattaaaatc atcatccata
     4141 gcttcgacaa aacgctctcg gtagaccgcc aaggctgcca atttttcgct gctctcatca
     4201 tctgattgct cgcttgatgc gggcagttta tttaacacgt cacgtaaatt atatactaca
     4261 ttgtttaaac gttccagccc actttttgct tgctgcagta aatccgcagc gtaatttatg
     4321 ggattacgat aatgtgctga aagcataaaa aagcgcacaa cttccggatc caccacctga
     4381 cgcaaatcgt ggacagtcat aaagttgcct agagatttcg acattttttg ctggttaata
     4441 tttaaataac cggcatgcag ccagtacttg gcaaaaggtc ggcctgtcgc accttcactt
     4501 tgtgcttttt cgttttcatg gtgcggaaaa ataaggtctg gccctccagc atgaatgtct
     4561 agggtctcac ccagatattt catagacatg actgaacatt cgatatgcca gcctggtctt
     4621 cccaagcccc aaggactctc ccaagccggc tctccgggct tttgtttttt ccacaggaca
     4681 aagtctaaag gatgcctttt gcgttcatca acttccaccc tcgccccagc taccaaatcc
     4741 tccagcggct gctgcgaaag ctccccgtag ctgtgaaaag cgggcgtatg gaaatacaca
     4801 ttcccatcca cattataggc catgcctttc gctattagat cttcaatcag cacaatcatt
     4861 tcctgaatat gttcagttgc ccggggatgc acatcagccg gccgcacacc cagcgcttgc
     4921 acatcctcaa agtaagcctg aatatagcgg tctgctaatt cctctaccgt gatgcccagt
     4981 tcagcagctc gtttgatcat tttatcatct atatcagtaa aattttgtac gaaagtcaca
     5041 tcataaccgc gccattgtaa ataacggcga attacatcaa aaacgataaa aatccgggca
     5101 ttgccaatat gaaagtaatc ataaacggta gggccacaag cataaatacc cactttattg
     5161 ccgttaacag gcacaaaatc ttccttctta cgtgtcacgg tgttaaagac gcgcatcgtc
     5221 attgtcttcc cccttcctta ataacttttc ttgtttaaag cgcaagtcat caatctgctg
     5281 ctgcaggcac cgcatcattt ctgccaccgg atccggtaaa tccacatggt ttaaatcaac
     5341 tgacggcacc cgctcaccct caaaaattac cactcgcccc ggtactccta ctactgtagt
     5401 atgttcaggc acattatgca gcactaccga accggcacca attttggcat tgtcaccaat
     5461 ttcaatggga cctagcactt tagcacctgt accaatgacc acactatgcc cgatcgtggg
     5521 gtgtcgtttc cctttttctt ttcccgtacc gcctaaagtg actccctgat aaagtgttac
     5581 attatcacca atttcggtgg tctcaccaat taccaccccc gtaccatggt caataaagaa
     5641 accttcgcca attttggcac cgggatgaat ttcgatcccc gttagaaagc gactaaactg
     5701 ggagattaaa cgcggcagca agactaaacc aagtcgatac aaggcatggg caagacgatg
     5761 tagccataag gcgtgtaagc caggatagca taataaaact tcaagaaaac tttttgccgc
     5821 cgggtcacgc tcaaaaatgg ctttaacatc cctcttaata tgaaacatct tgtcatcctt
     5881 tcccatacat ataaaagccg tctccttcag agacggctta accgcggttc caccctgttt
     5941 gggctatctg ccccactcac tcagtataac ggctgtgccg tacccaccta cttatttcag
     6001 tggctctgct caagggcgca cttcaggttg cctcactgaa agcttcttgc agccgcggaa
     6061 gcttctctct gtcagcctct tcaaccttac tcttcccctt cattgctttt acttatttag
     6121 ttattacata ttataaaagt ctgagcgaaa ggtgtcaata aaacatcttt agaggcgtgt
     6181 taaagtagca gttaaccttt tagctactgt ggctggtccc aatagcgcca agagggcatt
     6241 taaatctggc ccatgtgact gaccggaaac ggccacgcgc acagccatgt aaagtgcctt
     6301 gccttttaca cctagctctt tgccaatgcc ttttaagacc tgacgcacct gtgccacatc
     6361 ccaagcatac agagtctgta ctttcgctaa gagagcctta agcacagccg gggaggtttc
     6421 cagtgccaat acttcttggg cctctgcatc attttcatcc caactaactg tttcaccaaa
     6481 gaatacttgc agtaattgcg gcgcttgtgc cgcatactcc aatttctctt gaattgtagc
     6541 cacagtttgt tccaaccatg ctaatttatc tgccgtcaag tcagcctcta ccaaaccagc
     6601 tttttgcagg tgaggaagac aaagttttgt cagctccccc accggcagta ccttaagata
     6661 gtgggcgttc atccacttta atttttcgag attaaaaaca gcaggacttt tagaaacacg
     6721 ctgcaaagaa aagagacggg caatttcatc tatctcatag atttcttcct ctccctcggg
     6781 agaccagcct aacagcgaaa ggaagttaac taaagcttgc ggtaaatagc cctcatcacg
     6841 atattgtaca actgaagttg caccatgtcg cttactcatt tttgaacgat cctcacctaa
     6901 aattaaggag atatgagcaa actctggcag ctcaaagcct aacgcttcgt agattaagag
     6961 ctgacggggt gtattggaaa gatgttcttc tccgcgcagg atatgcgtaa tgcccatggc
     7021 tgcatcatca agcactacgg caaagttata agtaggcaaa ccgtccgatt tgacaataat
     7081 aaagtcaccg ataccgttgc tttcaaactc caccctgccc cgcaccaggt cgttgaccac
     7141 taaaagtttt ccttccggca cccgaaaacg gatggcaggc tttctgccct ccccacactt
     7201 ttcttcttgt tcctgcactg ataagtcacg gcatttgccc aaatagcgag gtgttttacc
     7261 ctgagccatg agttcctgac gttccgcttc caactcttct tcagtacagt agcaggcata
     7321 agcttgtcca gaggctagca actggtcgat aaatggttgg tacaaaggta gtcgctcctg
     7381 ctgacgatac ggtccattag gacccccaac gtcaatccct tcgtcccagt caatacccag
     7441 ccaacgcaaa gagtccaaaa ttaatttttc gtattcgcgt gtagaacgat ccaaatcggt
     7501 atcttcaatc cgaacaacca ttgtcccccc gtgatgccgc gcaaagagcc agttaaaaag
     7561 tgccgaacgg gcaccaccaa tatgtaaagg cccagtaggg ctaggcgcaa agcgtacttt
     7621 tactttagcc attccattcc ctcctacttc ttactgaaaa cattttaaaa cctttcctat
     7681 tatatcatct tataaaattg gaaacaacaa acagtgaagt atgttataat aatgaagaaa
     7741 tatttagatg aattgacggg aggctgagca tgaaaaactt tttcaacctt tacaaccatg
     7801 gctttgtccg cgtggctgtg gccgtaccgc aagtgcaggt agcagatccc acctttaatg
     7861 tgacagaaat tatcgcgcta gcgcagcagg cagcagcaca aaatgctgta ctcactgtct
     7921 tccctgaact aggcgtgacg gcttatagta acgaagatct atttcatcag caagccctct
     7981 tagagacagc agagcgcgaa gtacagcggc tgcttgctga aaccagtgag cttaatacca
     8041 tcctcatcgt cggtgctccg ctcttaattg attcctttct ttataattgt gcacttgtcc
     8101 tcctccgcgg ctgcctcctt ggcattgtcc ctaaatcgta tttgcccaat caacgggaat
     8161 tttatgaaag cagacaattc cgtcctacac aaacagctat caataatacc gtcgactatg
     8221 gccagcaaac cgccattccc tttggcagcg atcttaaatt taaagtagct aatctccccc
     8281 attttacttt tgctgtagaa atcggcgagg atttaactgt acccattcct ccttcaagtt
     8341 tcgcggccct agcgggagca acagtgattg ctaatcctgc cgcctctcct cttacagtag
     8401 gcaaagcagc ctctcgacac actttacttg ccgctcaatc tgcccgctgc cgagcagcct
     8461 atctttatgc ggccgcaggt ctaggtgaat caacgacaga tctggcctgg gacggtcacg
     8521 ctctgatcta tgaaaacgga caagccctag ccgaagcacc acgtttttct ttatcatcca
     8581 cactgacact gtctgaactt gatttggcag gtctgacgca agagcgcttg catgataaca
     8641 cctttgccga taactccctt ttactgcagg cccaaaacaa acactatcgt accattacct
     8701 gtgaaatccc catcccgtct gctaagctca tgctcacccg cactctgccc cgcttccctt
     8761 atgtccccgc caacaagacg gccttagcac agcagtgctt tgaagccatg caaattcagg
     8821 tccagggact agtaaaacgg ttacagtcca ccggcatcac gaaaattatc atcggtgttt
     8881 ccggtggttt agattccacc ctggcccttt tggttgccgt aaaagctata gacttattag
     8941 gcctgccacg ccacaatatc cgtgcttaca gcatgccggg ctttgctacc tctcaccgca
     9001 ccaaagctaa cgcttggcag ctaatgcaag cgcttggtgt ctatgctgaa gaattagata
     9061 tccgccccag cagccagcaa atgttagcag atattgggca cccggttacc cgtggcgaaa
     9121 cccgctatga tgctacctac gagaatgtcc aggcaggtca gcgtactgcc atcctttttc
     9181 gtctggctaa tttgcaccag gggctggtgc tgggcacagg tgatttaagt gaattggcgc
     9241 tgggctggtt aacctatggc gttggcgatc atatgtccca ctataacgtt aatgccagcg
     9301 taccaaaaac attaataagg gcactcctgc aatggcaagc tgaggccgaa gaaatagctc
     9361 agaaaactgc agctattatt ttagatgttt taaaaactga aattagccct gaattagtcc
     9421 caggtacaga tgacgataac caacccgctc aacatacaga agattatatc ggcccctatg
     9481 aattacagga ctttcacatt tactacactc tccgctacgg cttccgcccc agcaaaatcg
     9541 ccttcctagc ctacaacgcc tggggtgcta aatacagcct ggaggaaatt aaacattggc
     9601 tgacaatttt tctgaaacgc ttttttgcca gcagtcagtt taaacgctca gccatgccta
     9661 acgcccccaa agtagtttct acctctcttt caccccgcgg cgactggcgt gcccccagcg
     9721 acgctgtcgc caccgcctgg ctcaccgaac tcgcatcctg ggacctctaa cagtttgcga
     9781 caggggacgg ttccttgtcg cactttctca cgccttcgcc cacgggtggc tcggaagaag
     9841 cacttaacgt ctcaaattaa acctacaaaa tttaacgatc gagtaggagg ctacccatta
     9901 attgaatagc cgtcctctca caccaccgta cgtaccgttc ggtatacggc ggttcaatag
     9961 aattagtgga ctagtgaata tctttcactg attgacagaa gtcccagctt cttaag
//
LOCUS       DASRMS010000086        11444 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_88113, whole genome shotgun sequence.
ACCESSION   DASRMS010000086 DASRMS010000000
VERSION     DASRMS010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 11444)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11444)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11444
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88113"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            85..768
                     /locus_tag="VFC74_03585"
     CDS             85..768
                     /locus_tag="VFC74_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24450.1"
                     /translation="MNKDLTELIFILDRSGSMRGLESDTIGGYNGMLAKQKKEPGEAK
                     ITTVLFDDRYELLHNRLNVRSIKPITEKDYFVRGCTALLDAVGNTINTIINVQKHTAA
                     EERAEHVMFVITTDGMENASREFSYEQVRQLIEQQKSKYGWEFIFLGANIDAINTAER
                     FGISKDRATNFHADSKGTSLNYEVINETVSDLRASRPIAKDWKQRIDEDFKQRQTGQR
                     PAPAKRKNS"
     misc_binding    899..1133
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1192..2418
                     /gene="tyrS"
                     /locus_tag="VFC74_03590"
     CDS             1192..2418
                     /gene="tyrS"
                     /locus_tag="VFC74_03590"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HZK24451.1"
                     /translation="MKIYDELMARGLIAQVTDEEEIRELIDTGKATFYIGFDPTADSL
                     HVGHFMALSLMKRLQMAGNIPIVLIGGGTGHVGDPSGRTDLRNMMNEELIQHNCDCFQ
                     KQMEKFIEFGEDKAIMVNNADWLLKLNYVEMLREVGPHFSVNNMLRAECYKQRMEKGL
                     SFLEFNYMIMQAYDFYHLYQHYGCNMQFGGDDQWSNMLAGTELIRRKLGKNAYAMTIT
                     LLLNAEGQKMGKTASGAVWLDPEKTTPYEFFQYWRNTDDADVLKCLRMLTFLPLDQIE
                     EMDQWEGSQLNEAKEILAFELTKLVHSEKEAQQAKETSHALFIGGVDDAEMPTTEFTA
                     AQLTDGAIGILDLLLETGLAASKSEARRLVQQGGIHLDDIKVETIDQLITTTQLQAGV
                     KIRKGKKIYHKALLKA"
     gene            2538..3092
                     /locus_tag="VFC74_03595"
     CDS             2538..3092
                     /locus_tag="VFC74_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005808046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK24452.1"
                     /translation="MNHLGTKTLETKRLRLRRFEIDDAQAMFDNWASNEEVTKYLTWP
                     AHQSITVSQEYIKSLISRYEKLNTYEWGIELKEIRQVIGSLGVVRCNDEVGYVHVGYC
                     IGKQWWSKGITSEAFSAVIKYLMDEVKVNRIEARHDPRNFVSGKVMEKCGLKYEGTLR
                     ESDINNQGRCDAAWYSLLKTDYHL"
     gene            3305..4384
                     /locus_tag="VFC74_03600"
     CDS             3305..4384
                     /locus_tag="VFC74_03600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK24453.1"
                     /translation="MGKFMETAANRDYSKWWQAEGSFPWLIQKPKPPIEKRIIGLKDN
                     YIRCIKGNQPYWMPAYSYESATIWPDAMEEHPVPEVDGYDWWGVYWKMVDKAGGMITK
                     PGTRTFSDFSKWKEELEWPDLSLVDFEADGKKIQATLDPERPHIYQCVEGIFERLHEM
                     IPFDESLMAFYEEPELLEEFFQKMADYKIESCQNIFKYYGRVDGVLYHDDWGTQRDGF
                     FSNEMFRKQLFPATKRFLDYVKGEGKFVELHSCGKNVQYVPMMIEMGIDMWNPQSVIN
                     DPEFLYNTYGKEMSFSFPLFIKQNWSEAEVRKAVRDFVDHYGETGRVMCWLMVENRDA
                     AKDKIAKDELYNYSLAYYNKLYGRA"
     gene            complement(4515..5183)
                     /locus_tag="VFC74_03605"
     CDS             complement(4515..5183)
                     /locus_tag="VFC74_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24454.1"
                     /translation="MDRIVFDYIPIKFDTEEVLKFNKIRPNSSFREVFIEALSIAEKH
                     LRCKAVLKWVNIEAIVGKTTTIDGVNFQSKVMADNLQDVKKAFLFVMTVGAEIDECKE
                     IEENTLRDMIKGTALGYIHKFVLEYITEHFGYQDLAMMTPGSLPDWPIINNDDLFAMI
                     GNVTEDIGVSLNKHHYMQPYNSSSGIIFSSSTGYVNCTLCKNLDCIGRHAPFDQAEYN
                     RLFT"
     gene            5370..5900
                     /locus_tag="VFC74_03610"
     CDS             5370..5900
                     /locus_tag="VFC74_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YcxB family protein"
                     /protein_id="HZK24455.1"
                     /translation="MEENKLFTVETTMEKKDYRKFLYTATFLKSKGAIPYIFLFSAVV
                     AGLLVYQDSYFNVTQFIIYWAVLIGAAVLAVIFQVERRNKQRIATDKTGAFGAKERLD
                     FYEDFQVIKSTAFAGETSIKYSQFYQVLESRDYFITYFNMNQASLIRKEDLERETAEK
                     LRSLYQKKMGKNYKRI"
     gene            6025..7497
                     /locus_tag="VFC74_03615"
     CDS             6025..7497
                     /locus_tag="VFC74_03615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HZK24456.1"
                     /translation="MTAPKTIIRAGWNFDNSFARLPELFYSALKPTPVRAPQLVMLNL
                     PLAKVLGLDAEALQSDAGIAVLAGNRIPEGALPLSQAYAGHQFGHFTMLGDGRAHLLG
                     EHLTPQGERFDIQLKGSGRTPYSRGGDGRAALAPMLREYIIGESMHALGIPTTRSLAV
                     VSTGEAIRREVKLPGAMLTRVAASHLRVGTFQYAAQFADAAALKTLADYTIQRHYPAI
                     EAAENRYLLLLQAVIKRQAELIAKWQLVGFVHGVMNTDNMTISGETIDYGPCAFMDAY
                     DPGTVFSSIDLQGRYAYGNQPKIAAWNLARFAESLLPLLHDQQEEAIQLAQTALTEFF
                     TIYDHLYLAGMREKLGIFNEEEQDQALIDSLLGLMQKHSADYTNTFRALTINATEMEM
                     FAAAEFTTWSQQWQARLARQQENASSVRQLMQASNPAVIPRNHRVEEALSAAVEDEDC
                     AVCKRLVSVLDKPYAYTAEQDEYADPPPPSACPYRTFCGT"
     gene            complement(7582..11370)
                     /locus_tag="VFC74_03620"
     CDS             complement(7582..11370)
                     /locus_tag="VFC74_03620"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014255721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HZK24457.1"
                     /translation="MSNTIRRILVEKKPGFDIEARHLQQDLRDNLGITALTRVRLINR
                     YDLSGVTDAAYNQARTTIFAEPNVDFYYDETVEIENDVKYFAMEYLPGQYDQRADSAA
                     QCIQILTQGEPPTIQSAKLILLYGKLTGDDLQRIKAYCINAVDSREAALEKPASLAIR
                     TAAPADVSILDGFIQLDDTQLSAYKRSMGFVMSDEDLKFCQTYFRDKEKRDPTVTEMT
                     VIDTYWSDHCRHTTFSTSLEKITIEAKELGAPIKNAYEAYLQDRQAVYGTKQRDICLM
                     DIATLGMKKLKKEGKLQDLDQSDEINACSIVVKADINGIQEDWLVMFKNETHNHPTEI
                     EPFGGAATCLGGAIRDPLSGRVYVYQAMRVTGSGDPRASIAETLPGKLPQRKITTGAA
                     AGFSAYGNQIGLATGQVTEIYDQGFIAKRMEIGAVIGAAPKENVVRAKPTAGDAVLLL
                     GGKTGRDGCGGATGSSVEHTEESIYTCGAEVQKGNPPTERKIQRLFRNPQASQLIKKC
                     NDFGAGGVSVAIGELTDSLVINLDAVPKKYEGLDGTELAISESQERMAVVVAKENVSR
                     FKALAAAENLEAVEVAAVTADKRLKMLWRDKAIVDISRDFLATNGIQQHSDVYVEAPA
                     VTDNYFTKQLAAVPADLEEAWLNNLQNLNVCSQRGLVERFDSSIGAGTILMPFGGKYQ
                     ATPAIGMAAKLPVESGDTSTGTLMSFGYDAQLAKWSPFHGALYAVVEAVSKIVAMGGS
                     YQNIRLTLQEYFEKLGQDHKKWGKPFSALLGAHYAQMQLGIPAIGGKDSMSGTFKELN
                     VPPTLVAFAVNAVDVNDVISPEFKQADSQVVLITIARDAYELPDFATLGNTYQGITEL
                     IQAGKITAAYPLNNGGAAEAISKMCFGNRLGFTLAKEFNLAAEGKLLFSPDYGSMVIE
                     VPKDVGLQQDFAHLPYKLLGQTQQKETIELGEITISLDLAQEIWEAPLEKVFPTKTAV
                     TEKTMPQALTSKQRGSAKSKLRVAKPRVLIPVFPGTNCEYDTKKRFELAGAETKVFII
                     KNLTATAIEESIQAMAAAIKQTQIIALPGGFSAGDEPDGAGKFIATFFRNPWIRDAVM
                     ELLNNKDGLMLGICNGFQAFIKLGLLPYGEIKTIDDTDPTLTYNNIGRHMSGIVRTKV
                     TSVLSPWFNNVAVGDIHHIAISHGEGRFIANEAAMQKMIVTGQVATQYVDYQGQPSLD
                     IRFNPNSSHYAVEGITSPDGRILGKMGHSERIGSNLYINIPGEKDQLIFEAGVNYFTD
                     "
BASE COUNT         3230 a         2364 c         2647 g         3203 t
ORIGIN      
        1 ggtaaatgtc gcttgccaag cgaccgccag atcagcagca catgttatcc ttaaatcaca
       61 gtaaatgatc aggaggataa caagatgaac aaagatttaa cagaactgat ttttattctg
      121 gacagaagcg gttccatgcg tggtctggaa agtgacacca tcggcggcta taatgggatg
      181 ttagcaaaac aaaaaaaaga gcctggcgag gcgaagataa ctaccgtgct ctttgatgac
      241 agatatgaat tgctgcataa ccgcttgaac gtgcggagca tcaagccgat cacagaaaaa
      301 gattactttg taagaggctg cactgcatta ttagatgccg taggaaatac tattaacacc
      361 atcatcaatg tgcagaaaca tacggcagca gaagagcgtg ccgaacatgt gatgttcgtc
      421 ataactacag acggcatgga aaacgccagc cgcgagttta gctatgaaca agtacgccag
      481 ctgattgaac agcaaaaaag caagtatggt tgggaattta ttttccttgg agccaacatt
      541 gatgctatca acacggcaga acgttttggt ataagtaagg atcgcgccac caattttcat
      601 gctgatagta aaggtacttc acttaattat gaagtaatta atgaaacggt cagcgacctg
      661 cgggcaagtc gtcccatcgc caaggattgg aagcagcgta ttgacgaaga ttttaagcag
      721 cgccagacag ggcaacgtcc tgcgcctgcg aaaaggaaaa atagttagtg caaacaccat
      781 gcgatatttt ttgcgctgac gctggcaaaa tgttgagtcg catagtataa taagtctaat
      841 tactggcaaa attaattttc aggggaaaaa gcagtagaac cgtcccctga cacataatgc
      901 aaaagcgagg aaaggaagaa gtaggtcagt ttagtatctt ttagagagca tccggctggt
      961 gtaaggatgc agatgcggct gagacgaata catcctggag ctgcggcctg aatttattag
     1021 tagggcttga cgggtgcgcc cgctatagcg tttaagtgcg attgcactta atgaggggaa
     1081 ttccgtgagg gatttccaaa gcaaaggtgg taccgcaatt ttttgccctt tgttcccatg
     1141 ggaacgaggg catttttgtt tactttatga cttgaaaaag gagcgctgac aatgaaaatt
     1201 tatgacgaac taatggcccg tgggctgata gcccaagtga ctgacgaaga ggaaataaga
     1261 gaactcattg atactggtaa agcaaccttt tatatcggtt ttgatcccac agcagactct
     1321 ttacatgtgg gccattttat ggccctgagc ctaatgaaac gtttacaaat ggcaggcaac
     1381 atacctatcg tcctgatcgg cggtggcact ggacatgtgg gagatccctc tggacgtaca
     1441 gatctgcgta atatgatgaa tgaggaactt atccagcata actgcgattg ttttcagaaa
     1501 cagatggaaa agtttattga gttcggtgaa gataaggcta tcatggtgaa caatgcagat
     1561 tggctcttaa agctcaatta tgtggagatg ctgcgtgaag taggacccca tttttcagtt
     1621 aataacatgt tacgtgctga atgttacaaa cagaggatgg aaaagggact ttcgttcttg
     1681 gagttcaact atatgattat gcaggcctat gacttctacc atctctacca gcactatggc
     1741 tgcaatatgc agtttggcgg cgatgatcag tggtctaata tgctggctgg tacggaactt
     1801 atccgccgca aattaggcaa aaatgcctac gcgatgacaa tcaccttact attgaatgcc
     1861 gaaggtcaaa aaatgggcaa aacggctagc ggggcagtct ggcttgatcc tgaaaaaact
     1921 acaccctatg aattctttca gtattggcga aatacggatg atgccgacgt tctcaaatgc
     1981 ttacgcatgc taaccttctt accgcttgat caaattgagg aaatggatca atgggaagga
     2041 agtcagctaa acgaagctaa agaaatcctc gcgtttgaac taacaaaact agttcatagt
     2101 gaaaaagaag cgcagcaagc taaagaaact tctcatgctc tctttattgg tggtgtggat
     2161 gatgctgaaa tgccaactac tgagttcaca gcagcgcagc taacagatgg tgccataggt
     2221 attttggact tgctgctgga aactggttta gctgcctcta aaagcgaagc aagacgcctg
     2281 gtgcaacaag gcggcatcca tcttgatgac attaaagttg aaaccattga ccagctgatt
     2341 acgacaacac aattacaagc aggagtcaaa atacgcaaag gaaagaaaat ctaccataaa
     2401 gccctattga aagcgtgaca gcccgcgaca ggggacggtt ctttgtcgca caatagtgcg
     2461 ataatcacag caactttgat tgccctctgc gccaaactgt gaagttacgt gccacattgc
     2521 attattggga ggcgactatg aatcatttag ggacaaaaac acttgaaact aaacggcttc
     2581 gattacgacg attcgaaatt gatgatgcac aagccatgtt tgataattgg gcaagtaatg
     2641 aagaagtaac caagtatctt acgtggcctg ctcatcagag tattactgtt tcccaagaat
     2701 atattaagtc actaatcagt agatacgaaa aactgaacac ctatgaatgg ggaattgaac
     2761 taaaagaaat cagacaagtc ataggttctc tcggagtagt tcgttgtaat gatgaagtcg
     2821 ggtatgttca tgtagggtac tgtatcggta aacagtggtg gagtaaaggg ataacatccg
     2881 aagcattttc agctgtaatc aaatacctga tggacgaagt aaaggttaac cgcattgagg
     2941 cccggcatga tcctcgtaat ttcgtatcag gtaaagtgat ggagaaatgt ggtctaaaat
     3001 acgaggggac attaagagaa tctgatatta ataatcaggg tagatgtgac gctgcttggt
     3061 atagcttgct taaaacggat taccacctgt gacaggggtc ggttcttgga cacgatttta
     3121 agcgaggatc ttgcagatag ttgagtggaa cctgatggcc gctagcgata acttttactt
     3181 ttggaattgc cgactgctaa aaactttgat agaatataat tgttggaact tatgcactat
     3241 tgctactcat gaatatagct tctttagttc cattctctaa aatattgcaa agaaggggat
     3301 atgtatgggt aagtttatgg aaactgcagc taatcgggat tattcaaagt ggtggcaagc
     3361 ggagggaagt ttcccttggt taattcaaaa accgaaacca cctattgaaa aaagaataat
     3421 aggccttaaa gacaattaca tacgctgcat caaaggcaac caaccttact ggatgcctgc
     3481 atactcttat gagtctgcaa ctatttggcc agatgctatg gaagagcatc ccgtgcctga
     3541 agttgatggc tatgactggt ggggtgtgta ttggaagatg gtggacaaag caggcggtat
     3601 gattactaaa ccaggtaccc gcacattttc tgatttttct aaatggaaag aagaactaga
     3661 atggccggat ttgtctttag tcgattttga agctgatggc aaaaaaattc aagcaacgct
     3721 tgatccggaa agaccgcata tctatcaatg tgtggaaggt atttttgaac gcctgcacga
     3781 aatgattcct tttgatgaaa gtttgatggc tttttatgaa gaaccggaat tgttagaaga
     3841 attcttccag aagatggccg actacaagat tgaatcatgc cagaatattt ttaagtacta
     3901 tggcagagta gatggtgtct tgtatcatga tgattggggt acccaaagag acggattttt
     3961 ctctaatgaa atgttccgta agcagctctt ccccgcaaca aagcgctttt tagactatgt
     4021 aaaaggcgaa ggtaaatttg ttgagctgca ctcctgcggt aaaaacgttc agtatgtgcc
     4081 aatgatgatt gaaatgggta ttgatatgtg gaatccgcag tcagtgatta atgatcctga
     4141 attcttgtac aatacctatg gcaaggaaat gtcattttct ttcccacttt ttattaagca
     4201 gaactggtca gaagccgaag ttcgcaaagc tgtacgcgat tttgtcgatc actatggtga
     4261 aacagggcgt gtaatgtgct ggctgatggt agaaaacaga gatgctgcaa aagacaaaat
     4321 agccaaggat gaactttata attactcctt ggcttattac aacaaactat atggtagagc
     4381 atagcaactt atcttgactt agattacaaa acctctggtc gtttatagga ccagaggttt
     4441 tattttaaag taaatgattc gcttagcctg ccacctagtt aagtgaagtg tgacaaagaa
     4501 ccgtcccttg agtcttaagt aaagaggcgg ttgtattctg cttggtcgaa gggggcatgg
     4561 cggccgatgc aatcaaggtt tttgcaaaga gtgcagttaa cataacctgt gctgctggaa
     4621 aagataatgc ccgaacttga attgtagggc tgcatgtaat gatgcttatt tagtgaaaca
     4681 ccgatatctt cagttacatt gccaatcatg gcaaagagat catcattgtt aataatcggc
     4741 cagtcaggca gcgagccggg agtcatcatg gccaggtctt gatagccgaa atgctcggta
     4801 atatattcaa ggacaaactt atgtatataa ccgagggctg tgcctttaat catatctcgc
     4861 aatgtattct cttctatttc tttgcattca tcaatttctg caccaacggt catgacaaag
     4921 agaaaagctt tctttacatc ttgcagatta tccgccatta ctttactctg gaaattaaca
     4981 ccatcaatag tagtagtctt gcctacgatt gcctcaatat tgacccattt tagtactgct
     5041 ttgcagcgca gatgcttctc ggcaatgctt aatgcctcga taaatacttc tcggaaactg
     5101 gaattgggac gaattttatt aaacttcagt acttcttccg tgtcaaattt aatgggaatg
     5161 taatcaaata ctattctatc caacgtctta ccccctgctc taaagtagaa taacattaac
     5221 ttcttcttct aaaatatcac taaggtacag aatattcaac tgaatatttc ctcataccaa
     5281 ggagtaggag aactgcttga taaagtagaa attaaagata gcggcacctt agagtggttg
     5341 taaagtagaa aaaaagggtg gtaagcaaga tggaggaaaa caaactcttc accgtagaaa
     5401 caaccatgga aaaaaaagac taccgtaaat ttttatacac agcaacgttt ttaaagagta
     5461 aaggagcgat cccctatatt tttttatttt ccgctgtcgt ggcgggactg cttgtttatc
     5521 aggacagtta ctttaatgta actcaattca ttatttattg ggctgtctta ataggagcag
     5581 cagtgctggc tgtaattttt caagttgaaa gaaggaacaa acaaaggatt gccactgata
     5641 aaacaggggc ttttggtgct aaagaaagat tagattttta tgaagatttt caagtaataa
     5701 aaagtacagc ttttgctggg gaaactagca ttaagtatag ccagttttat caagtgctag
     5761 agtcaagaga ttactttatt acctatttca atatgaacca agcctcttta attagaaaag
     5821 aagatctgga gcgggagact gcggaaaaac tacgttcctt atatcaaaag aagatgggca
     5881 agaactacaa aagaatataa acaatgtaga ttaatttaag tgccgcctct cctctctcga
     5941 caggcatatt ctacagctat gtgcaagaaa ctagagttgt cttgattatt aggatacgtt
     6001 taaaaaattg tagaaggaga taagatgacg gcgccaaaaa ctataataag agcaggctgg
     6061 aattttgaca acagttttgc ccggctgcca gaattatttt atagtgccct caagccaacg
     6121 cctgtacgtg caccgcagtt ggtaatgctc aacttgccat tggccaaagt actgggtttg
     6181 gatgctgaag cactacaaag tgacgctggt atagctgttt tggccggtaa cagaataccc
     6241 gaaggagctt tacctctctc gcaggcatat gccggacatc aatttgggca ctttaccatg
     6301 ttgggtgatg gccgtgctca tttactgggc gagcatctta cgccccaggg tgagcgcttt
     6361 gatatccagc ttaaaggttc ggggcgcaca ccctattcgc gtggtggtga tggccgcgcc
     6421 gcccttgccc ctatgctgcg tgaatatata attggtgagt ctatgcatgc gctaggcata
     6481 cctacgaccc gcagcttggc tgtggtgagc acaggcgaag caatcaggcg tgaagtcaaa
     6541 ctccccggtg cgatgcttac ccgtgtagca gccagtcatc tgcgcgtagg tacttttcaa
     6601 tatgctgcac agtttgcaga tgcagcagct ttgaaaactt tagcggatta tacgatacaa
     6661 aggcattacc cagcaataga agctgctgaa aatcgctatc ttctcctgct gcaagcagtg
     6721 attaagcggc aggctgaatt gatagccaaa tggcagttag ttggttttgt gcatggggtg
     6781 atgaacactg acaatatgac cattagtggt gagacaattg attatggtcc ttgtgcgttt
     6841 atggatgctt atgacccagg cacagttttt agctctattg atctccaggg acgttatgct
     6901 tatggcaatc agccaaagat tgcagcgtgg aacttggcac gttttgcaga aagcctgctg
     6961 ccgctcttgc atgatcagca agaagaggcc atccaattag cacaaactgc tctcactgaa
     7021 tttttcacta tctatgatca cctttatctg gctgggatga gagaaaaatt aggcatcttt
     7081 aatgaagaag aacaggatca agccttgatt gacagtctgc ttggcctgat gcagaaacat
     7141 tctgctgact atactaatac ctttcgtgcc ctgacgatca acgccacaga gatggagatg
     7201 tttgcggctg ccgaatttac cacttggtct cagcagtggc aggcgaggct agccagacag
     7261 caggaaaatg caagttccgt gcggcagcta atgcaagcca gcaaccctgc ggtaatacca
     7321 cgtaaccatc gtgtggagga ggcattgtcc gctgccgtag aagatgaaga ctgtgctgtg
     7381 tgcaagcgac tggtcagtgt gcttgacaaa ccttatgcct acactgctga acaagatgaa
     7441 tacgctgacc caccaccgcc ctctgcctgc ccctatcgta ccttttgtgg tacttaaacg
     7501 gtaatatgtg ataaatatag ttttctgatc gaacaaaaaa agatcagcgc tcaactaatt
     7561 actttagccg agcgctgatt attagtctgt aaagtagttt acccctgctt caaagataag
     7621 ctggtctttt tcgccgggaa tgttaatgta gagattgctg ccaattcttt cagaatgccc
     7681 catcttccct aaaattctgc cgtcgggact ggtgataccc tcgacagcgt aatgagaact
     7741 gttggggttg aagcgaatat ctagtgaggg ttgtccttga taatccacat attgggtagc
     7801 gacttggcca gtgacaatca ttttctgcat ggctgcttca ttggcaatga agcggccttc
     7861 tccatgggag atagcgatat ggtgaatatc gcccaccgct acattattga accaagggga
     7921 gagtacagaa gtcactttgg tgcgtacgat accagacata tggcgtccaa tattattata
     7981 ggtgagtgtg ggatcagtat catcaattgt tttgatttca ccataaggta ggagacctag
     8041 cttgataaaa gcttggaagc cattgcagat gcccagcatc aggccgtctt tgttatttag
     8101 cagctccatc acggcatcac gaatccaagg attgcggaaa aaggtggcaa taaatttgcc
     8161 agcaccatca ggttcgtcac cagcactgaa gccgcctggc agggcgatga tttgcgtttg
     8221 ttttattgcc gcggccatag cttgaattga ttcttcgata gcggtggcag tgaggttttt
     8281 aataataaag actttagttt ctgcccccgc cagttcaaac ctctttttag tatcatattc
     8341 acaattagta cctggaaata cagggattag aacgcgtggt ttggcaactc gcagttttga
     8401 ttttgcagac ccgcgctgtt tagaagttaa agcttggggc atagtttttt ctgttactgc
     8461 agttttagtg ggaaagactt tttctaaggg tgcctcccaa atttcttggg caagatcgag
     8521 tgaaatggta atttcaccaa gctcaattgt ttctttttgc tgcgtctgcc caagcagctt
     8581 gtacggcaga tgagcaaaat cttgctgcaa gcctacatcc ttaggtactt caatcaccat
     8641 actgccgtaa tctggtgaga aaagaagctt accctctgca gctaggttga attctttagc
     8701 gagagtaaag ccgaggcgat tgccaaagca catcttacta attgcttcag ccgcgccccc
     8761 attgttgagg ggataagcag ctgtaatttt acccgcctga attaactccg tgatgccttg
     8821 gtacgtgttg cctagagttg caaagtcagg cagctcgtaa gcatctcttg cgatggtgat
     8881 taagacaacc tgactgtctg cctgcttgaa ttcaggtgaa attacatcat tcacatctac
     8941 agcattaact gcaaaggcaa ctagtgtggg tgggacatta agttccttaa aggtcccaga
     9001 catactgtct ttaccgccga tggccgggat tcccagctgc atctgggcat agtgagcacc
     9061 tagcagggca ctaaagggtt taccccattt tttatgatcc tggcctagtt tttcaaagta
     9121 ctcttgcagt gttagtcgaa tgttttgata actgccaccc atggccacga tcttggagac
     9181 tgcttctacc acagcatata aagcgccgtg gaaggggctc cattttgcca attgtgcatc
     9241 atagccaaag gacatcagcg tgcctgtgct ggtatcacca ctttctaccg gcaatttggc
     9301 cgccatgcca attgccggtg tggcttggta cttgccgccg aagggcatta gtatagtacc
     9361 agcgccgata gagctatcaa agcgttccac caagccgcgt tggctacaga catttaagtt
     9421 ttgtagatta ttgagccaag cttcttctag gtcagcgggg accgctgcta gctgcttagt
     9481 aaaatagtta tctgtaactg ccggtgcttc tacataaaca tcgctatgct gctgtatacc
     9541 attagtagcg agaaagtctc tactaatgtc aacaatagct ttgtcccgcc aaagcatttt
     9601 tagacgcttg tccgcagtga cggctgcaac ctctacggct tcgagatttt ctgcagccgc
     9661 caaagcttta aatcgactaa cgttttcttt ggctacaact acagccatac gttcttgtga
     9721 ctctgagatc gctagttcag taccatctag tccttcatat ttcttgggta ccgcatctaa
     9781 attaatcacc agactatcgg ttaactcgcc gatggccaca gaaacaccac cggctccaaa
     9841 gtcattgcat tttttaatta gttggctggc ttgggggttt ctaaacagtc gttggatttt
     9901 tctttctgtc gggggattac ccttttgaac ttcagcgcca caggtgtaaa tagattcttc
     9961 agtatgctcc acagaggagc ctgtggcacc gccacaccca tcgcggccag ttttgccgcc
    10021 tagcagcagc acggcatcgc cagcggttgg tttggcacgg actacgtttt cctttggtgc
    10081 tgcaccaatg actgcaccta tttccattcg tttagcgata aagccttgat catatatttc
    10141 agtaacctga ccggtagcta agccaatttg attgccatac gcgctgaacc ctgctgctgc
    10201 accagtagta attttgcgtt ggggcagctt gccaggcagt gtctcggcga tacttgctct
    10261 cgggtcgccg ctgccagtta cccgcatggc ctgatacaca taaacacggc cagaaagggg
    10321 gtctctgatg gcgcctccta aacaagttgc tgcgccaccg aagggctcaa tttctgtagg
    10381 atggttatgg gtctcgtttt taaacattac tagccaatct tcttgtatac cattaatgtc
    10441 tgcttttacc acaatactgc aagcattgat ttcatctgat tggtctaaat cctgcagttt
    10501 gccttctttt tttagctttt tcatgcccag cgttgcaatg tccatgagac agatatctct
    10561 ttgttttgta ccgtaaacag cttgacggtc ttgaagatat gcttcatagg cattctttat
    10621 aggtgcccct agttccttgg cttcgattgt aatcttctca aggctggtag agaaggtagt
    10681 atgtcggcag tgatctgacc agtatgtatc aatcaccgtc atttctgtga cggttgggtc
    10741 tcttttctct ttgtctctaa agtaggtttg acaaaacttt aaatcttcgt cactcatcac
    10801 aaaccccatg cttcttttgt aagcggaaag ctgtgtgtca tccagctgta taaatccatc
    10861 taggattgac acatctgctg gggcagctgt tcttatggcc aaagaagctg gtttttctag
    10921 ggcggcttca cgagagtcaa ccgcattgat gcagtaagct ttaatccttt gcagatcatc
    10981 tcctgttagc ttgccataaa gtaaaattaa ttttgctgat tgaatggtcg gcggttctcc
    11041 ctgagttaag atctggatac attgagcagc agagtctgct cgttgatcgt attgcccagg
    11101 taagtactcc atggcgaaat acttaacgtc attttctatc tctacggttt catcataata
    11161 gaagtctacg ttgggttcag caaagattgt tgttcgggct tggttgtagg ccgcatctgt
    11221 tacaccggaa agatcgtaac ggttaattag tcgcactcgt gttaaagctg tgatgcccaa
    11281 attatccctt aaatcttgct gtaaatgtcg tgcctcaata tcaaaacctg gttttttttc
    11341 tactaatatt cttctgattg tattgctcac ttgacccacc tcgtttctag cataattatc
    11401 gctgcttgcc gaaaagagca ttgttatcat tcattatata atat
//
LOCUS       DASRMS010000087         1090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_692567, whole genome shotgun sequence.
ACCESSION   DASRMS010000087 DASRMS010000000
VERSION     DASRMS010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1090
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_692567"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1090
                     /locus_tag="VFC74_03625"
     CDS             <1..>1090
                     /locus_tag="VFC74_03625"
                     /inference="COORDINATES: protein motif:HMM:NF016906.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NosD domain-containing protein"
                     /protein_id="HZK24458.1"
                     /translation="FTDTITTINGKTGAIVKADIVALGIPAQDTVVDISGKIDKVAGK
                     GLSTEDYTTAEKTKLAGIAASANNYSHPPTHPASMITQDANNRFVTDAEKAVWNSPPG
                     TTGKKVARFVVGTSTAGWTDADCDYLCDGTADQTEINAAITALPATGGEIIILDGTYN
                     ITAKIDVTKDNVSIRGNGNATILKRMFNSTSTPGVIALTGRSGCNVANLQVDGNSASF
                     ANLVNSGISLDSSSNNTIIGNTCNNNNSYGIRITDSSNNTVTGNTCSSNEIGILLFAS
                     SNNTVTGNTCTRGTGLPTDYTASQNTIWLGGTGNNYNLISNNNCMGKAPRVDGGTGNS
                     VYGNKYDSGNYKDFTQDEKTKLAGISEEA"
BASE COUNT          360 a          248 c          240 g          242 t
ORIGIN      
        1 gtttacagac actattacaa ctatcaatgg caagacaggt gcaatcgtca aggccgatat
       61 tgtggctctg ggaatccctg cacaagatac tgttgttgac atcagcggaa agattgataa
      121 ggtcgctggc aagggactaa gcactgagga ctataccaca gcagaaaaaa caaagcttgc
      181 aggcattgct gcaagtgcca acaactactc gcatccacct acgcatccag caagtatgat
      241 tacgcaagat gcaaacaacc gctttgttac ggacgctgaa aaggcggtgt ggaatagtcc
      301 accaggtaca accggcaaaa aggttgctcg ctttgtagtt ggaacatcta cagcaggctg
      361 gactgacgct gattgtgatt acctttgcga cggtacagca gaccaaacgg aaataaatgc
      421 tgcaataacc gctttacctg caactggtgg cgagattata atcttagacg gaacttacaa
      481 tattaccgca aagattgatg tcactaaaga taatgtaagc atcagaggaa atggcaatgc
      541 cactatacta aagaggatgt ttaactccac ctcaacaccc ggagtaatag ctctaactgg
      601 cagaagtggt tgcaatgttg ctaatttgca agtagatgga aacagtgcaa gttttgccaa
      661 cctcgtcaat tctggcatct cattagactc ctcaagcaat aataccatca taggcaacac
      721 ctgcaacaat aacaatagtt atggcatccg tataaccgat tcaagcaaca acaccgtcac
      781 tggcaacact tgcagctcca acgaaattgg catcttatta ttcgcctcaa gcaataacac
      841 cgtcactggt aacacctgca ctcgtggcac tggtttgcca acggactaca ctgcgagtca
      901 gaatacaata tggctcggag gtacgggcaa caactacaac ctgatttcaa ataataactg
      961 catgggcaaa gcgccaaggg tagatggcgg cacaggcaac agtgtctatg gtaataagta
     1021 tgattctgga aactataagg attttacaca agatgaaaaa acaaaacttg cgggcatatc
     1081 agaggaagca
//
LOCUS       DASRMS010000088         4659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_357134, whole genome shotgun sequence.
ACCESSION   DASRMS010000088 DASRMS010000000
VERSION     DASRMS010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4659
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357134"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..650
                     /locus_tag="VFC74_03630"
     CDS             204..650
                     /locus_tag="VFC74_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24459.1"
                     /translation="MIGKTLRNHDGVALITVIGVFVIIMIISLSVATLFANNLKQVTH
                     QEYQTRAYYLALSGVDLAYEALVKEPADEDEDSLLDEFIGGSLTELEDQFTIGEDRVV
                     ITISAFDTGSTREVKIHSIGTVVDTGNSESLTLIFEAENPLVRRWQ"
     gene            689..2101
                     /locus_tag="VFC74_03635"
     CDS             689..2101
                     /locus_tag="VFC74_03635"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZK24460.1"
                     /translation="MKLRNNRGLTIVELMIVLALLSMVIAPAYMLLGLGNRVHSSSIE
                     EYELQSTARLAAAHINRAARFSSAIFTIPRSSFQEDNLSAGWSYIGVLDGAVVAIEYS
                     ATGHHKTELVPASDNVTYEIVFKKVLEDHQEKIVGFSINGYIDGRPVKTDEYGQPLGH
                     ISIDSQAEALNSLQVIHKGTALDPAVAIAFRRENRDKPEIEATLPVAQIAMVLDVSGS
                     MEYQMNGTTTNTEANRRITKLKSSSKKLVEQFAASEHPVYVSLVPFATSANNPKPFRP
                     VQTDISALYNDINSLTAGGGTNTGDGIRRAYHGIINGRNNADFVGKEVSDYIIILVDG
                     VTTFGSVQSNSNRSWVTHHNNVNEGFLDRGWLEFSANGQIAGNGSELDLTWGEGYVQN
                     IGSMVKSDGNIKVYVIGFSSRSADFGSLANIAASTGALPTSSGQSFFEAGDEEELDLV
                     FGEIQKEIINNLWHIDGPNL"
     gene            2137..4602
                     /locus_tag="VFC74_03640"
     CDS             2137..4602
                     /locus_tag="VFC74_03640"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZK24461.1"
                     /translation="MLKNKGFTLVEVLIAIALIMIISVTFVLSMTTHFSWLSSAKAMT
                     QSVFAAQQSMETEIEGIKQALLDGVTPDDKVSLTLFNGKPQQITVEGYYREIEIANNR
                     VVRAIVADSRMAEYPVPIVNKATLKLRKDNADLARNYDYVIRPGLSARATSEVADPHN
                     VFLLNRHEWFVSRPGFNVPMVADDYIDEDFDHGRLYPIFPHDYDPVPIKANSDMLSQS
                     DLTALPSEYTGRHIIYTITPYAMSGKKGTTVFSDPVYLHGPTVTNGLVLHLDASAISK
                     DPAVSSIVNSGDNIFVSEWHDLSGSNNHAVQSVESQKPELTEVQYQDDLYVWGKSLQE
                     NNAGTKMRVSSFSPTTLNQFTAIIVAKSSAIQPNTNIIKGGNNTWSFEWNEVGNLAFA
                     AGGIPAELVGHQALDDEWHIFSAVVSDNNISLRVDGELEGYGTAANSVSSRPLEINWA
                     NIDIAEILVYNRDLASGLDLLSVETYLKNKYNPDPDEVHSTIAYLKPMAPKTIIQGES
                     FTPPSTVDAVMTNGTTQKVVVTWSGTIDTSTIGTQSITATANMDETKTTFLVVNVVGI
                     ERLIPHTEDAITLRTREYINLPSRLDAVLTNGTTRAVDVTWSSSVPEATVSETILTGH
                     TLGYYPDSLTASAVLDPSKTAIYSVEVTGIAVTGVSVTPTSLEMYIADTSSLTATVAP
                     ADATDQSITWSSSSTSVATVDNNGLVTAHVPGSTTITVETLDGGFTATCEVTVRYHPV
                     TVVGYEARNIQTYRSGFLNLNYHTSADIYIILSDGTEHFHGNVSASRVLSYPTINTWV
                     TGNATADSSGESVSYNLNITVSP"
BASE COUNT         1335 a          971 c         1029 g         1324 t
ORIGIN      
        1 tcatcaaaaa atgtctgctt tttgtgggct aatcatataa tattttcata agttaaacat
       61 tatgtcttga aatttgttga ctaacttttt ttttaggtat ataatggtaa aaagggtgat
      121 attttgttta cttttaaaga cacgttcttt attttactgg aaaagtaagt atctgctgta
      181 gctagcaaaa ggacgggatg tagatgatcg gcaaaacact ccggaatcat gatggtgttg
      241 ctttgattac tgttattggt gtctttgtta ttatcatgat tatctcatta tcagtagcaa
      301 cactttttgc caataaccta aagcaggtta cacaccagga gtatcagaca cgagcttatt
      361 atttagcctt gtcaggtgtt gatctggcct atgaagccct agtcaaagag ccagcggatg
      421 aagatgaaga ctccttgtta gatgaattca tcggggggtc tctgacagaa ctagaggatc
      481 aatttacaat tggtgaggat cgtgtagtta ttacgataag tgctttcgat actggttcta
      541 cgcgtgaagt taaaattcac tctataggta ctgtagtaga cactggcaac agtgaatcac
      601 ttaccctaat ctttgaagca gaaaatcctt tagttcgccg ctggcagtag cttgtttgta
      661 aaattctcaa taaggatggg tgagaattat gaaattaagg aataaccggg ggctcactat
      721 tgttgagttg atgattgtcc ttgctctgct gagcatggtg attgcaccag cctatatgct
      781 gttgggttta gggaacaggg ttcatagttc cagtattgaa gaatatgaac tgcaatcaac
      841 tgccaggctg gctgcggcac acatcaatcg tgctgcacgt ttttcttccg caatctttac
      901 tattcccaga tccagtttcc aagaggacaa cctatcagct ggttggagtt atatcggcgt
      961 gctggatggg gccgttgttg ctattgaata tagtgccacg ggtcaccata aaacagaact
     1021 ggtcccagcc agcgataatg ttacctatga aatagttttt aaaaaagtac tggaagatca
     1081 tcaagaaaaa atagtaggtt tttctatcaa tggttacata gatggccgac ctgtaaaaac
     1141 ggacgagtat ggacaacccc tgggacatat tagtatcgat tcgcaggctg aagcgttaaa
     1201 ctcactgcag gtgatccata aaggcactgc cctggatcca gctgtggcca ttgcctttag
     1261 aagagaaaat cgtgataaac cggaaattga ggcaacttta cctgttgccc aaattgcgat
     1321 ggttttagat gtgtccggca gtatggaata tcaaatgaat ggtacgacta caaatacaga
     1381 agcaaaccga cggattacaa agttaaagag ctcctcaaag aaattggttg agcagtttgc
     1441 tgcttcagag caccctgtat atgtttcatt agtgcccttt gctacttcag ccaataaccc
     1501 taaacccttc cgcccggttc aaactgacat cagtgctctt tataacgata ttaattcgct
     1561 tactgccggt ggcggcacca atacgggaga tggaattcgt cgcgcttatc atggtattat
     1621 aaatggacgc aataatgctg attttgtggg taaagaggtc agtgattata tcattatttt
     1681 agtagacggc gtaacaactt ttggcagtgt gcaaagcaat agtaaccgca gctgggtaac
     1741 tcatcacaac aatgttaatg aaggattttt agatagaggc tggttagagt tttctgctaa
     1801 cggtcaaatt gccggcaacg gttccgagct ggatctgact tggggcgaag gctatgtgca
     1861 gaatatcggt tcaatggtga aaagcgatgg taatattaag gtttatgtaa ttggtttctc
     1921 atctcgcagc gcagacttcg gcagcttagc caatattgcc gccagtactg gtgcactgcc
     1981 tacttcttct ggccagtcct tttttgaagc gggagatgaa gaagaattgg atcttgtttt
     2041 cggtgagatt caaaaagaga ttatcaataa tctatggcat attgacggac ctaatttata
     2101 gggcaacaaa gcttaatgcc agaaaggagc cttttgatgc tgaaaaataa aggctttact
     2161 ttagttgagg tactcatagc cattgctttg attatgatta tttctgttac ctttgtctta
     2221 tcaatgacga cacacttttc ctggctatcc agcgccaaag ccatgacaca aagtgttttt
     2281 gcggcacaac aatctatgga aacagagatt gagggaatca agcaagctct tttagatggt
     2341 gtaacacctg atgacaaggt ctcgctaacc ttatttaacg ggaaaccgca gcagatcact
     2401 gtcgaaggtt attaccgtga gattgaaatc gccaacaacc gggtagttcg ggcgattgtt
     2461 gctgatagca gaatggcaga atacccggtg cctattgtca acaaggccac tcttaagtta
     2521 agaaaagata atgccgattt ggcgagaaat tacgactatg ttatccgccc aggtttgtca
     2581 gccagagcaa catcagaagt agctgatccg cataatgttt ttctcctgaa ccgtcatgaa
     2641 tggtttgtct cgcgtccggg ttttaacgtg ccgatggttg ctgacgacta cattgatgag
     2701 gatttcgacc atgggcgttt atatccgatt ttccctcatg attatgaccc ggtaccaatc
     2761 aaagccaatt cagatatgct cagccaaagt gacttaacgg cactgcccag tgaatatact
     2821 ggccgtcata ttatttatac cattacaccc tatgctatgt ccggcaaaaa gggaacgaca
     2881 gtctttagcg atcccgttta tctgcatgga ccaactgtaa ctaatggctt agttctgcat
     2941 cttgatgcct cagctatcag caaggatcct gctgttagct cgatagtaaa tagcggtgac
     3001 aatatttttg tttcagaatg gcacgatctt tcaggcagca acaatcatgc agtacaaagc
     3061 gtagaaagcc aaaaacctga attgactgag gtccagtatc aagacgattt atatgtctgg
     3121 ggtaaatcct tgcaggaaaa taatgctggc actaagatga gagttagttc tttcagtccg
     3181 accacactta accagtttac agccatcatt gtcgctaaaa gcagtgctat tcaacccaac
     3241 actaatatta ttaaaggtgg caacaatacc tggagctttg agtggaacga agttggtaat
     3301 ctagcttttg ctgcgggagg catccctgct gaactggtag gccatcaagc cctcgacgac
     3361 gagtggcata tcttttctgc tgttgtctct gacaataata tctctttgcg cgtagacgga
     3421 gaactggaag gctacggcac tgctgccaat tcagtctcca gcagaccttt ggagatcaac
     3481 tgggctaaca tagacattgc agagatttta gtttacaatc gtgaccttgc ctcagggtta
     3541 gatttattat cagtcgaaac ctatttaaag aacaaatata atccagatcc tgatgaagta
     3601 cactccacta ttgcttacct caagcccatg gcaccgaaaa ctattattca gggagaaagc
     3661 tttacacctc caagtacagt tgatgccgtc atgactaatg gcaccacaca aaaagttgta
     3721 gttacttgga gcggcaccat agatacatca actatcggca cacagtctat taccgctaca
     3781 gcgaacatgg atgagacaaa aactaccttc ttagtggtta atgtcgttgg tattgaacgc
     3841 ctaatcccac atactgaaga tgctattacc ttacgaacca gagaatatat taacctgccc
     3901 tcaagactgg atgccgtctt gaccaacggc acgacccgtg ctgttgatgt gacctggagt
     3961 tccagtgtcc ccgaagcgac agtttccgag accattctca ccggtcacac tctgggttat
     4021 tatccagaca gccttaccgc ctccgctgta cttgatccta gtaagacagc aatatattcg
     4081 gtagaggtta ccggtattgc cgtcactggt gtctcagtta cacctacctc cttagagatg
     4141 tatattgctg atacaagcag cctaacggca acagtggcgc ctgccgatgc aacagaccaa
     4201 agtattacat ggagcagcag cagcacctca gttgccacag ttgataacaa tggcttagtc
     4261 acagcacatg tacccggtag tactacaatc accgtagaga cgcttgatgg tggatttact
     4321 gctacctgtg aggttacggt ccgataccac cctgtaacag ttgtaggtta tgaggccaga
     4381 aatatacaaa cttacagatc aggtttttta aatctaaact atcatactag cgctgatatt
     4441 tacattatcc tcagtgatgg tacagaacat tttcatggaa atgtttcagc ttctcgggtg
     4501 ttgtcatatc ccacaataaa cacatgggtt actggcaatg ccacagctga ttcttcagga
     4561 gaaagtgttt cttacaacct aaatattact gtgagtccat aagacgagtt attatgctaa
     4621 gcgtgcctga ccaagacagt caggcacgct taactttat
//
LOCUS       DASRMS010000089        58503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_759855, whole genome shotgun sequence.
ACCESSION   DASRMS010000089 DASRMS010000000
VERSION     DASRMS010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 58503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58503
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759855"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(918..1301)
                     /locus_tag="VFC74_03645"
     CDS             complement(918..1301)
                     /locus_tag="VFC74_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3825 domain-containing protein"
                     /protein_id="HZK24462.1"
                     /translation="MIKFEHIIIERLERFPEQFEHFDVTSLARLLDSAIDYGIAMAER
                     NYKLVIPQYRPQRDKIQFLLPLYLGGEFTKSPDLALVLDLEKNIYTPETILPLDAAYQ
                     NARVIAKPDALWLDPQEIVENQCDE"
     gene            complement(1625..2197)
                     /locus_tag="VFC74_03650"
     CDS             complement(1625..2197)
                     /locus_tag="VFC74_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HZK24463.1"
                     /translation="MIKLIAITTMLIDHIGFAFFPEMAILRLIGRIAFPLFALELARG
                     RRRTRSIKKYLTRLLVLAILSQVPYMLFFDTTDLNILFTLFVSLLMLIALDNKIYLAA
                     AGLFALTVMFSFEYGYYGPLTVFLVDKIDLQDIKYTIIYFAVNIFSGFQGFALLAYPL
                     FKIRDKKILPTWLAYGFYPGHLLVLYFLLH"
     gene            complement(2417..3277)
                     /locus_tag="VFC74_03655"
     CDS             complement(2417..3277)
                     /locus_tag="VFC74_03655"
                     /inference="COORDINATES: protein motif:HMM:NF024748.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HZK24464.1"
                     /translation="MKHEELDKARQNFNKLNMLLARKAKNRGLQTSLCLYDFLTKQWI
                     EKDADNRLYPASLIKILYLLTVLDQVEQGVLALNDSYTFSEQDKYAGSTKVTGAGSLQ
                     FTEAGGIYTVEELLKMMISASDNVAANIVLDLVGPQAVAAMTTRLGLSQTRGTRKMYA
                     LDSPLPPNSSTARELTELLIALENREVCGEKLKDLAVSMMLHTENKNRIGRYLKSNSV
                     LVANKVGTVDHMVGDMALIYFPSRPPVALTIMVTIPPCRLAIANALARRAAARTISRL
                     ASLVVKELQK"
     gene            complement(3455..3550)
                     /locus_tag="VFC74_03660"
     tRNA            complement(3455..3550)
                     /locus_tag="VFC74_03660"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3513..3515),aa:Ser,seq:gct)
     gene            complement(3586..3672)
                     /locus_tag="VFC74_03665"
     tRNA            complement(3586..3672)
                     /locus_tag="VFC74_03665"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3636..3638),aa:Ser,seq:tga)
     gene            complement(3733..5013)
                     /gene="serS"
                     /locus_tag="VFC74_03670"
     CDS             complement(3733..5013)
                     /gene="serS"
                     /locus_tag="VFC74_03670"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HZK24465.1"
                     /translation="MLDLKFVRDNLDIVRQALHDKGETAAIDRFNEVDGRRRDLLQEV
                     EQLKNKRNLVSAEVARMKKAKEDASTVIEEMRGVAQRIKELDSQTREAEKDLHEILLT
                     LPNIPDASVPVGQSEDDNIPIRHVGDVPNFNFTAQPHWDIATNLDIIDFERAGKVTGA
                     RFAFYKGWGAQLERALVNFMLDLHTGEHGYTEIFPPFMVHRDSMVGTGQLPKFEEEAF
                     RVADTDYFLIPTAEVPVTNLHRDEIFTAAELPRCYVAYSACFRAEAGAHGRDTRGLIR
                     QHQFNKVELVKFVRPEESMTELEKLTVNAEEVLKRLGLPYRTVLMCTADLGFAAAKKY
                     DLEVWLPSYNTYREISSCSNFGDFQARRAGIRFRRLQGGKAEFVHTLNGSGVAIGRTV
                     AAILENYQQADGTVAIPEALCPYLPQIKSGYIGK"
     misc_binding    complement(5107..5312)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5459..6022
                     /locus_tag="VFC74_03675"
     CDS             5459..6022
                     /locus_tag="VFC74_03675"
                     /inference="COORDINATES: protein motif:HMM:NF013346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM165/GDT1 family protein"
                     /protein_id="HZK24466.1"
                     /translation="MQAFLIAFAATLLAEMGDKTQLVTLTLSTRYPPLRVLAGSLSAL
                     AAITGLAVLLGEYLAQLFPEMVTMLASGLFFIVVGIFLLLKKEEKEDADVRTSGRGIT
                     LQTFFLVFLAELGDKTQLTAIALTAAYGSPLAVFLGAMAGQTVNHSLAAYLGARFLSR
                     LPARTIKIGSALLFIIFGLIFLYTALR"
     gene            complement(6193..7887)
                     /gene="argS"
                     /locus_tag="VFC74_03680"
     CDS             complement(6193..7887)
                     /gene="argS"
                     /locus_tag="VFC74_03680"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HZK24467.1"
                     /translation="MQTFREEIIRLLSPHVPLNKQQISEDLETPPNPAMGDFAFPCFK
                     LAKAMRQAPPKIAVTLVEALPASALFERIEAKGPYLNFFCDRQKLACLTVEKVLSEKK
                     AYGHQQAGQGKTIVIDFSAPNIAKPFGVGHLRSTVIGNSLYRLYEALGYRCIGINHLG
                     DWGTQFGKLIVAYLKWGNEKQLQKDPITYLYELYVRFHQEAEHEPQLNEAARSWFKKL
                     EDGEPQAQDLWQRFRALSLIEFKRIYQILDVEFDSYSGESFYNEMLDDTIKLVAEQGL
                     SSESEGALVVDLSAEGMPPCLLRKQDGATLYATRDLCAAIYRWQQYNFEKMLYVVGAD
                     QALHFQQFFTVLRKMGYSWAEQCFHVPFGLIGYKDGKMSTRQGTLIFLEEVLQRATEL
                     AAQIIQDKNPSLANKAEVARQVGIGAVIFGDLSNDRIKNIEFDWDKVLDFSGETAPYV
                     QYAHARICSILRKAPAWPADYDAALLTTDEEQAVIGLLARFTDTITRAAKTYKPSIVA
                     RYVVDLAAEFNRFYHQCPVLNAEEELRTARLALIDAVRQVLVNGLYLLGIAAPEEM"
     gene            complement(8017..10068)
                     /locus_tag="VFC74_03685"
     CDS             complement(8017..10068)
                     /locus_tag="VFC74_03685"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZK24468.1"
                     /translation="MKKYTKKLIIAPILLLTLLLTGCISELTNNGKPIEPIVAGAEEV
                     RSPAVGQFNVKNSELQITDISVTADGLFTVVGTASKVVYLLKKEGKPVWERQLQSIPT
                     QTYIAPDGSFIALGTEGGQLTLIGPDQKEKASRQFDAPLTQLSIAKDGSLIAAVLHTD
                     HEEQQDQLVVMEKSCNVLWQEELGTIISAAVTGIDKRVVVNWREGEIPYFGVFSAEGE
                     LLWKLQEGTAISVDSSGQNIISGFSAEISRYDYKAEKVWEYAVSGEVSRVQVSDNGLY
                     VGVIITDPATQHQNLLYFSMTGEKLWQQVLPVGANVVVSADGSQVIVASWGQFQDDVT
                     KITEYDQQGQQINVLDVTGRAQKMAFASQSGTLVVGLYDGSIYFLDLYGKAPGTDTTK
                     EPKTTDYYQPVVFGRQEGENNVTLYFYDQDSRYLIPVTRSVKSSQSLLRGSILELVRG
                     PAQGSELQRTIPKESEIKVEVENGTVEVDLPEELNQMSGSTFLTGVLDSLLLTVSSIP
                     SVEQIQFTVAGEERITFGQEGLLIEEPFQPQPFSKKSGERLLFLPSASGRKYYLRPVT
                     KEFTPLKERALAEAVVQTVLTGYEDCFEKVLTLQSVKIADNIIYVDLTPSLHDLVSQS
                     IAAAARAAMLRDALALSLVENLPYSTVKITVNGTTPKSEKHYLPWEVTVIRPYYVNME
                     N"
     gene            complement(10154..11575)
                     /locus_tag="VFC74_03690"
     CDS             complement(10154..11575)
                     /locus_tag="VFC74_03690"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK24469.1"
                     /translation="MLNSIHTKLTLTYIILIVVVLVLTSLFLLNTLEQYYFAYQYEAM
                     TRAAKLIGGFVAPKLVNTPDITDLSNVAVDFSRQISARVLITDQKQHVLGDSQRVDGL
                     VGMNLERPEIAAALEQEEDHSWSVQQSEMSGEWVLQVAVPIINKGKVNGAVFTASSLS
                     YIHGVQRDIRNYLIAATLLAIVVASLLGRFFAQKLSSPIKALTLATEQMTRGDLAQHV
                     PVRTKDEIGRLTIQFNEMAQQLAISTRQLKDFVANASHEMRTPLTSLNVLIKSMREYS
                     LDPHEQEEFLGDIDQELERLIHLVEELLDLSRLDRVTADDTMAFTDIVPTIRTTLEML
                     EKRAQVKQIAFSYELPPRAAPVFAVLHQIKQIVFNLVDNAIKYTPANGQIWVKLTEAT
                     DSLKLIVSDTGVGIPAEHREKVFERFYRVDKARSREQGGTGLGLSIVYEIVKRHGGKI
                     WVEDSYQGSGSTFVVTLPKLPSV"
     gene            complement(11568..12266)
                     /locus_tag="VFC74_03695"
     CDS             complement(11568..12266)
                     /locus_tag="VFC74_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK24470.1"
                     /translation="MANILVVDDEKTIAKGLKFALEKEHNEVRVAYDGKEALRLFHEQ
                     TPDLIVLDLMLPEMDGFEVCRNIRKTSEVPIIMLTARGEDIDKILGLELGADDYMTKP
                     FNPRELVARVKAILRRSQAQPVDAAGMQVIRLQDLQIDFFQHKVRVHDKDVDLTSKEF
                     ALLSLLASHPGRVFSREKLLEQVWGYDYYGDARTVDVHIRHLREKIEPDPATPQLILT
                     VWGAGYKFREGKNA"
     gene            complement(12281..12928)
                     /locus_tag="VFC74_03700"
     CDS             complement(12281..12928)
                     /locus_tag="VFC74_03700"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZK24471.1"
                     /translation="MREKYLFDYLVSTTEAEHVTSTPVILAQAKGKDTTLAALAQTVV
                     NCQLCQLRQAAKQVIFGSGNPQAKLLLVGEGPGAEEDEQGQPFVGAAGKLLTKILASV
                     DISREEVYITNVVKCRPPGNRAPTTGEIETCLPHLLAQIELLNPKIVLCLGAVAAKAL
                     IDPQARVSVVRGKWVERHGRHWMATFHPAALLRDQTKKRPVWHDIQKVRDIYRSL"
     gene            complement(13105..13986)
                     /gene="pdxS"
                     /locus_tag="VFC74_03705"
     CDS             complement(13105..13986)
                     /gene="pdxS"
                     /locus_tag="VFC74_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HZK24472.1"
                     /translation="MAEQGTMHVNAGLTEVHKGVIMDVTTPEQAKIAEAAGAIAVMAL
                     ERVPADIRAAGGVARMANPDIILSILDTVSIPVMAKARIGHFAEAQILAALGIDYIDE
                     SEVLTPADEEFHINKKLFKVPFVCGARNLGEALRRIGEGASMIRTKGEPGTGNVVEAV
                     RHMRRVMSEIRKLQNLPDEELMTFAKKISAPYDLVVEVKRLGRLPVANLAAGGIATPA
                     DAALMMQLGCDGIFVGSGIFKSKDPTARARAIVEATLHYDDPAFLAEVSKGLGEAMPG
                     LEISSIKQEERMQERGW"
     gene            complement(14081..15142)
                     /locus_tag="VFC74_03710"
     CDS             complement(14081..15142)
                     /locus_tag="VFC74_03710"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HZK24473.1"
                     /translation="MKKKIAVLYGGRSGEHEVSLRSAAAIMEGLKNLPDIEVLPVFIT
                     KEGSWQLNGSLVTVLPDPDINGLYILDGERAGNTVTIDVIFPALHGTYGEDGTVQGLL
                     ELVRIPYVGAGVAGSAVGMDKILMKAALVAAGLPVGDYLWFTAYQWHQEQAHWLAAVE
                     EKLGFPCFIKPANLGSSVGISKAYNQQELRHAIEVALQYDRRVIVEKFLTGQEIECSV
                     LGNDDPQASLPGEIIPSNDFYDYRAKYIDDRSRIVIPAQLKPDTITKVRQLAVETFTT
                     LDCAGLARVDLFVNEQTGEIWLNEINTMPGFTTISMYPKMWEASGLPFAELLQQLIQL
                     AEERFQQKEALKTSFELDE"
     gene            complement(15262..17343)
                     /gene="fusA"
                     /locus_tag="VFC74_03715"
     CDS             complement(15262..17343)
                     /gene="fusA"
                     /locus_tag="VFC74_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZK24474.1"
                     /translation="MKNYTTEMIRNVALISHGGAGKTSLAEAMIYTSGAINRLGKVDA
                     GTTTTDFDSEETKRNITINTSLAPVEWNGVKINLLDTPGYFDFIGDVASALRVADAAV
                     VVLSATAGVEVGTENVWEFAEENKLPRLVFVNRMDKENANFGKVFAQLQEFFGLNVVP
                     VQLPIGAEAAFKGVVDLVKMKAYSFSDDGKKMTEAEIPAELAAEAAEYREKLMEAVAE
                     SDDDLLMKYLEGEPLTDDEVNAGLRTGTLNGKIIPVMCGAATANIGTQPLLEMIVTAF
                     PSPADIAEVTGEDAESGEKVTVKIDPQGALSALVFKTFADPFVGKISYFKVYTGTLKG
                     DNQLHNANKNKDERLGQVFTMLGKNQINVDQVAAGDIAAVAKLQVTETGDTLCDKQLC
                     TVFPPIAFPEPVTTFAVEPKKQGEEDKVAAGFSRFLEEDPTFRMERNAETKQTLISGM
                     GELHLEIITSKLASKFGVEVDLTLPRIPYKETIRGTAKVEGKHKKQSGGRGQFGHVWI
                     EFEALQDGEEFEFVDKIFGGSVPRQYIPAVEKGLRESLESGVVAGYPVVGIKASLYDG
                     SYHSVDSSEMAFKIAAHQAFKKGLEQAKPVLLEPVMYVEVTVPDQFMGDIMGDMNSRR
                     GRILGMEQSNGNQVIKTHVPMSEMLKYAIDLRSMTQGRGTFAMKEDHYDEVPAHLSEQ
                     IIADAKAAKEE"
     gene            complement(17602..18096)
                     /locus_tag="VFC74_03720"
     CDS             complement(17602..18096)
                     /locus_tag="VFC74_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZK24475.1"
                     /translation="MQGMVWKFGNDVDTDAIIPARYLNTSEASALAQHVMEDADPTFA
                     QKVTPGSIIVALKNFGCGSSREHAPIAIKAAGISCVIAHSFARIFYRNAINIGLPILA
                     CPEAAEKIAAGDEIRVDLENGTIYNLTSKETYQAAPFPPFMQNIMAKGGLIGYVQWRL
                     QQQQ"
     gene            complement(18097..19359)
                     /gene="leuC"
                     /locus_tag="VFC74_03725"
     CDS             complement(18097..19359)
                     /gene="leuC"
                     /locus_tag="VFC74_03725"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZK24476.1"
                     /translation="MGMTIAEKILARHADKTTVQPGELINAKVDMVLGNDITSPVAIN
                     EFRRLGVEKVFDRDRIALVPDHFTPNKDIQSAQQAKMLREFAREYDITYYFEVGRMGI
                     EHALLPEMGLALPGEIIIGADSHTCTYGALGALATGVGSTDMAAAMALGEAWFKVPST
                     LKFVFNGRLQPWVSGKDLILYLIGKIGVDGALYRAMEFTGEMISALSMDSRLSMSNMA
                     IEAGAKCGIIAPDQVTLDYAAARAKRDFTPVYADQDAAYEKIFTFQGEDIEPQVAFPH
                     LPENTRGLSEVGEVRIDQVVIGSCTNGRMEDLRIAAEIMRGNKVAKNLRVIIIPGTQN
                     ILRQAIQEGLVDIFLEAEAVVSTPTCGPCLGGHMGILAEGERALATTNRNFVGRMGHP
                     GSEVYLSNPAVAAASAITGKISAPREVL"
     gene            complement(19389..20390)
                     /gene="ilvC"
                     /locus_tag="VFC74_03730"
     CDS             complement(19389..20390)
                     /gene="ilvC"
                     /locus_tag="VFC74_03730"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HZK24477.1"
                     /translation="MAKMYYDNDANLNVLKGRTVAVMGYGSQGHAQAQNLQESGVDVI
                     VGLRKDSQSWAAAEAAGLKVMTVAEAAQTANVIQILLPDETQRQVYEAEIKPYLQDGD
                     ALGFSHGFNIHFQQILPPTNVDVFMVAPKGPGHLVRRTYTEGAGVPALLAVYQDYTGK
                     ASELGLAYAKGIGGTRAGVIETTFKEETETDLFGEQAVLCGGVSELIKAGFETLVEAG
                     YQPEIAYFECLHEMKLIVDLIYEGGLSYMRYSVSDTAQYGDFTRGKRIVTEETRQEMK
                     QILHEVQTGKFALEWIQENQAHRPFFKATDRMEKDQLLETVGKKLRRMMSWIAAKEV"
     gene            complement(20371..20895)
                     /gene="ilvN"
                     /locus_tag="VFC74_03735"
     CDS             complement(20371..20895)
                     /gene="ilvN"
                     /locus_tag="VFC74_03735"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HZK24478.1"
                     /translation="MKHVLAVLVEDKPGVLVRVAGLFARRSFNIESLAVGRTQDPGIS
                     RMTIEVEADERTLEQVEKQLHKLINVLKISNLTLEPSVHRELVLIKVKATAVNRAEIQ
                     QIVETFRAKVVDVALNSLIIEATGNEEKLQAIQLLLAPYGILEVVRTGKIALLRGSKT
                     TKNGEDLKNGKNVL"
     gene            complement(20892..22571)
                     /gene="ilvB"
                     /locus_tag="VFC74_03740"
     CDS             complement(20892..22571)
                     /gene="ilvB"
                     /locus_tag="VFC74_03740"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HZK24479.1"
                     /translation="MKMTGAEMVVAALKEEQVNVIFGYPGGKVLSLYDAFYEADIQHY
                     LARHEQGAIHAADGYARVTGQPGVVFTTSGPGATNLVTGLANAYMDSVPLVVFTGQVA
                     TTLIGTDAFQEADIFGITLPVTKHNYLVTDVNELPRIIKEAFYLAKTGRPGPILIDLP
                     TDVQVATGEFTYPQAVNIPGYNPTLDGHPGQISRAAQAMAQAKKPIIYAGGGVVISGA
                     DELLLALAEKAEIPVTTTLTGIGGFPGTHRLSLGMLGMHGTYYANMAVHEADLIIAVG
                     ARFDDRVTGKLSHFARTAKIIHIDIDPAEIGKNVSVHVPIVGDVTRVLTTLLGKVKPQ
                     THEAWLAQIAEWRKQAPLTYLQPEEGEILPQYVIQSIYQVTNGEALIATEVGQHQMWA
                     AQYYLFKKPRAFVTSGGLGTMGYGLPASLGLQIGAPGQTVFCIAGDGSLQMNLQELAT
                     AVAYNLPVKIAIINNQYLGMVRQWQEMFYGRRYSSVNLQQSPDFVKLAEAFGAVGLRV
                     QKPQEVIPAIKKALAIPGPVVIDFHVRAEENVFPMVPPNSGISEMVRGHEA"
     gene            complement(22568..24241)
                     /gene="ilvD"
                     /locus_tag="VFC74_03745"
     CDS             complement(22568..24241)
                     /gene="ilvD"
                     /locus_tag="VFC74_03745"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HZK24480.1"
                     /translation="MRSDKMKKGVERAPHRSLLYAMGWVKEEMERPLIGIVNSANDFV
                     PGHKHLHQISAAVKEGVRLAGGTPMEFFTIGVCDGLAMNHQGMHYSLASRELIADTVE
                     VMVQAHPLDGLVFIPNCDKIVPGMLMAAARLNLPAVFVSGGPMLAGRFQGQPADLNSL
                     FEAVGRVKAGTMAADELLELEESACPGCGSCSGMFTANSMNCITEALGMGLPGNGTVP
                     AVAAARVRLAKKAGIEVMKAIHNELTPAKILTTEAFVNALTMDMALGCSTNTVLHLPA
                     IAHEAGITLDLDYIQTISERIPQLCKLAPAGEYHIEDLAAAGGIQAVLKRLAEKDLLH
                     TACLTVSGRTLGEQLAEAAILDEDVIRPFTNPYSDSGGIAILRGNLAPDGAVVKQGAV
                     APEMMQKTGPARVFDSEEEAVEAIYGGKIQAGDIIVIRYEGPKGGPGMREMLTPTSSV
                     AGMGLDKDVALITDGRFSGATRGASIGHVSPEAMERGPIAVIAEGDLISIDIPARKLE
                     LLLSDDQLQQRLAKLTLPQPKITSGYLARYAQQVTSANTGAVFREVDAT"
     gene            complement(24259..25149)
                     /gene="ilvE"
                     /locus_tag="VFC74_03750"
     CDS             complement(24259..25149)
                     /gene="ilvE"
                     /locus_tag="VFC74_03750"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HZK24481.1"
                     /translation="MGRIIYLNGQYVPEEEAKVSVFDHGFLYGDGVFEGIRSYQGKVF
                     RLEEHLARFYESAKSIMLTIPESYAEMKEAILKTIRANNLQDAYIRAVVSRGTGDLGL
                     DPRKCEKATTVIIASSISLYPEELYEQGLSVITVATRRNAPDALDPQIKSLNYLNNIL
                     VKIEANRAGVMEAVMLNGQGYIAEGTGDNIFIIKNNKVITPPSYVGALPGITRDVIME
                     LAAAAGYTVLERPFTRHELYVADESFLTGTAAEVIPVVEVDGRQIGAGKPGSITNDLI
                     ARFRAVAGAEGEPVYPEDLA"
     misc_binding    complement(25361..25585)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(25682..25861)
                     /locus_tag="VFC74_03755"
     CDS             complement(25682..25861)
                     /locus_tag="VFC74_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HZK24482.1"
                     /translation="MKEFLSDKNLEYVEYNVEDNPEARQRMIDKSQHTIVPTVVVGEE
                     VVLGCDTQKLADLLP"
     gene            complement(26024..26569)
                     /locus_tag="VFC74_03760"
     CDS             complement(26024..26569)
                     /locus_tag="VFC74_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014354747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZK24483.1"
                     /translation="MVTMAMLAALSILLVYMVRFPIFPAAPFLEYDMADVPILIGTFL
                     FGPISGLILTVVVSLIQAVTVSAGSGWIGAIMHIIATGAFVLVAGTIYHKFTSRGGAV
                     VSLILGSIAMTLAMIPLNLVLTVAFMGAPREVVMGMLVPIIIPFNLIKAGANSVITFA
                     VYKAVARVLRLEIAETKEQTI"
     regulatory      complement(26736..26866)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(26929..28260)
                     /locus_tag="VFC74_03765"
     CDS             complement(26929..28260)
                     /locus_tag="VFC74_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZK24484.1"
                     /translation="MSHPKMKELRAAILTALLLFSLLLVLLVRFSWKNEIPDYPPLPY
                     SVDVVVVGSGLAGELTAIAAAQQDTDVLYLNLQQTEQQIVPPYVPTFWFAARSPGLTA
                     EAEPAYLPETMALEIYTRGKEAGNYAQILNFCLQSAASLQWLESLTGLDLVNEAKTNQ
                     VFHKLTARQLSELCQETAARLPKLVRAEKDWLPLRLKQQGNRVKGLVVRTAEGQELDI
                     SARAVVLADGGFASNQELLLDYAGLKGTRPRLEGGHQGVGLQLAQALGGITQNMSQVW
                     LQPVIVQDGARIEQLIWQEALLFNAAGQVLTGDNLTKILLRANGTVFVVAGAGNAVAQ
                     QIKTQTIDDVNLLAAVLNSEPAAVAEQVKQLKAPYQVAVLGLVALTPGGLTVSEDYAV
                     MGQQGALEGLYAAGELTTGLHGRQAIPELFLTEAVVSAKIAGEQAAQWAKR"
     gene            complement(28272..30719)
                     /gene="gyrA"
                     /locus_tag="VFC74_03770"
     CDS             complement(28272..30719)
                     /gene="gyrA"
                     /locus_tag="VFC74_03770"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HZK24485.1"
                     /translation="MNFTHGKIVPIAIHDEVKSSFIDYAMSVIVSRALPDVRDGLKPV
                     HRRILYSMADLSMWPDKAHKKSARIVGEVLGKYHPHGDTAVYDAMVRLAQNFSIRYPL
                     VDGHGNFGSVDGDPPAAMRYTEVRMGKIAVEMLADIQKDTVDFRPNFDDTLQEPVVLP
                     AGFPNLLVNGSTGIAVGMATNIPPHNLREVIDALVYLIDNPECEEQEIFSRIKGPDFP
                     TGGLIVGKEGIRSAYRTGRGIIKIRSLTQIEHMENGKSRIVVTELPYQVNKAKTIEKI
                     ADLVRNKKIEGITDLRDESDRNGLRVVIELRRDVNAQVILNQLFKFTQLQQTFGIIML
                     ALVNGRPKVLNLRQMLSCYLDHQKDVITRRTQYELRKAEERAHILEGLRIAIDNLDAV
                     IKLIRASHTVDLARQGLMDNFQLSERQAKAILDMRLQKLTGLEREKLEEEYLELIKKI
                     AYYQEILANEKLLLQIIKDEQLQIRDKYGDERRTKIVVSEDDFLIEDLIAEEDMVVTL
                     THNGYIKRIPATTYKKQRRGGRGVTAMQTKGDDFVEHVFVASTHAYVCCFTNRGKMYR
                     KKVFELPEAGRQARGTAIINLMAVEPGELITAVIPVRDFDAGQYMFMATRQGVVKKTA
                     LEDFANARKNGIIAISLRDDDELIDVRLTDGEHQIALVTQDGLSIRFNENDVRSMGRN
                     AYGVRGISLGQDDQVIALEVVHPQLERDSELLVVTENGFGKRTPFFEYRTQTRGGKGI
                     YTIRVMDKKGAVIGARIVRPGDEVMIITAGGIVIRQDATEISQQGRHTQGVTLIRVDE
                     GDRVVSLARVVADDQDA"
     gene            complement(30735..32666)
                     /gene="gyrB"
                     /locus_tag="VFC74_03775"
     CDS             complement(30735..32666)
                     /gene="gyrB"
                     /locus_tag="VFC74_03775"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HZK24486.1"
                     /translation="MAEKQDKIKNEIHYGADQIQVLEGLEAVRKRPGMYIGSTGERGL
                     HHLVYEVVDNSIDEALAGFCDNILVTIEPENIITVTDDGRGIPVGEQKKMKKPAVEVV
                     LTVLHAGGKFGGEGYKVSGGLHGVGVSVVNALSEWLEVEVQVDGKLHQMRFERGKTVS
                     PLKIIGKTKKTGTKISFRPDPEIFESLNFDFDTLTQRLRELAFLNKGIRITLVDRRSE
                     EEVKEVYKYDGGIKSFVEYLNKNKDLLQKKVIYLERAKEDQSEIEVALQYHDGYNELI
                     FSFANNIRTHEGGTHEAGFKTALTRIFNDYARKQNLLKENENNISGEDIREGLTAVVS
                     VKLLVPQFEGQTKTKLGNTEMRGLVDSFLYEELGTYFEENPAIAKRLVEKAISAARAR
                     EAARKARELARRKSALEITSLPGKLADCVSRDPAISELYIVEGDSAGGSAKQGRDRRF
                     QAILPLRGKIINVEKARLDRILGNEEIRTMITALGSGIGADMDVSRARYHKIIIMTDA
                     DVDGSHIRTLLLTFFFRYMQPLLQAGYIYIAQPPLYKLKKKQKEYYLYSDQDLERLLK
                     EIGRDGITLQRYKGLGEMNPDQLWSTTMDPEKRTILQVTMEDAMRADEIFTILMGDKV
                     EPRRNFIEKHAQYVRNLDI"
     gene            complement(32820..33068)
                     /locus_tag="VFC74_03780"
     CDS             complement(32820..33068)
                     /locus_tag="VFC74_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix/biofilm biosynthesis
                     regulator RemA family protein"
                     /protein_id="HZK24487.1"
                     /translation="MFLHIGGSRIVRAKDIIGIFDIRLKDKQCNKEFLQSAKQITKQD
                     SEETKTFIVTESSVLFSPIAPTTLRKRFQAKIFETIEK"
     gene            complement(33122..33679)
                     /locus_tag="VFC74_03785"
     CDS             complement(33122..33679)
                     /locus_tag="VFC74_03785"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HZK24488.1"
                     /translation="MIPIAQILKKTLKNLPAAKKIRGQMLIDAWSEVVGEWIAMKTEA
                     LAFENGTLLVRVCDSVWAQHLSLQKTQIIAKLQRATKTRVLHDLRFQVGKLQTQAAAN
                     NSHPEEGNWRSISLGQQELASIEDSFDGSNLPAELKQSMRALFVAQKKHQKWVIEQGE
                     PSCEHCGLPLVQTARETFCLSCKRK"
     gene            complement(33654..34787)
                     /gene="recF"
                     /locus_tag="VFC74_03790"
     CDS             complement(33654..34787)
                     /gene="recF"
                     /locus_tag="VFC74_03790"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HZK24489.1"
                     /translation="MRVETLVLHNFRNYSHLELKLDAHLNILVGENAQGKTNFLEAVN
                     FSALARSFRTRNEEELVRWGSESCTVVASVQQRLQLQKLRVSYDLQTRKKKYFCNGVE
                     LSRAAYLGRVIPVLFTPEDLAVVKGSPQLRRKFFDELIGKISPLYEQDLSRYQQILRQ
                     RNQLLKTQREKVLHSAELASWNEQLIKQGSKVIAKRSFAVHRLGLLARLAHRSLTDRE
                     ETLTVDYLSSIGEKITSPNTDWEEKFAHALDKHAGHECRMGQTLIGPHRDDILLAIND
                     KNARIYASQGQQRTLILALKLAEIEFIKSETGEFPILLLDDVFSELDSKRRQLLVAAI
                     EGKVQTIITGTAAERLGVLHHDGKLFIVQGGEVRTSDPHSSNS"
     gene            complement(34810..35913)
                     /gene="dnaN"
                     /locus_tag="VFC74_03795"
     CDS             complement(34810..35913)
                     /gene="dnaN"
                     /locus_tag="VFC74_03795"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HZK24490.1"
                     /translation="MRLLLPKNLLASHLQIVAKAMPTRSVNPALEGVLFECDGEKLTL
                     TATNLELGIQTQFAAAHKERGQVVLPGKTVEIIRHLPGDSVLIEVNPENFTTQISSAP
                     AEFQVYGYSADDYPNFPEQQPELAQCSFRIKVADLRRALRQTLFCVSHDEGKPAFIGV
                     FFSLRQHELTLSASDTFRLATTTCAVESKSDLEFLVPGKNLNEVLRVFTEDEAMIEAA
                     VMQNQLFLNCNNICISSRLIDENYPNITRVIPKSFTGKAIVETAAFTQAVERASLLAE
                     GNNHVLRFLINKDTMVMRASSKYGKIQEVVPIEFNGEEVEIAFNARFMLEMLKITEGD
                     QCLIELNGHEQPFIMRDSLYDNYLYLILPVKIS"
     gene            complement(36237..37610)
                     /gene="dnaA"
                     /locus_tag="VFC74_03800"
     CDS             complement(36237..37610)
                     /gene="dnaA"
                     /locus_tag="VFC74_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017210024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HZK24491.1"
                     /translation="MEFELLDIWQATLDQIETKMSKPSFETWLKTTKPVSINSESLVI
                     SVPNDFTKDWMQGRYADLISHALQEVVGNENYRVHFVTPRINDDDGKDFNQEEVVASP
                     PTRRPEKPTSTLSTSWLNPKYTFNTFVIGNSNRFAHAASLAVSEAPARAYNPLFIYGG
                     VGLGKTHLMHAIGHHVTDNTPANRVMYLSSEKFTNEFINSIRDNKTVEFRNKYRTIDV
                     LLIDDIQFLAGKETTQEEFFHTFNALHENNKQIIISSDRPPKEIPTLEDRLRSRFEWG
                     LITDIQPPDLETRIAILRKKAFSENITMDDDVIHYIANNIVSNIRELEGAFIRVIAYS
                     SLTKRRIDIPLAQETLQTILATHEKPKIITIERIQKVTAEHFALKIEEMKAKKRTKNV
                     AMPRQIAMYLARELTDSSLPKIGEEFGGRDHTTVMHAHKKIISDLKKNPQLQSIVEQL
                     TDKINSC"
     gene            38216..38350
                     /gene="rpmH"
                     /locus_tag="VFC74_03805"
     CDS             38216..38350
                     /gene="rpmH"
                     /locus_tag="VFC74_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011342949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HZK24492.1"
                     /translation="MKRTYQPKVRKRKKIHGFRRRMSTPAGRLVIKRRRAKGRKRLSA
                     "
     gene            38365..38721
                     /gene="rnpA"
                     /locus_tag="VFC74_03810"
     CDS             38365..38721
                     /gene="rnpA"
                     /locus_tag="VFC74_03810"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HZK24493.1"
                     /translation="MKKNGRIRKNSEYQHVFTKGRSLAMRALVLYQLPNQEKENRTGF
                     VVSKKVGNAVTRNRVKRLFREAYRLHAADLVQGKDLVFIARPQAAKLDYQQAATAMKQ
                     ILKRGGLFSVNKSAQV"
     gene            38747..38980
                     /gene="yidD"
                     /locus_tag="VFC74_03815"
     CDS             38747..38980
                     /gene="yidD"
                     /locus_tag="VFC74_03815"
                     /inference="COORDINATES: protein motif:HMM:NF013932.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HZK24494.1"
                     /translation="MQRLLLLFIRFYRKFLSPFKTASCRFTPTCSQYAKEAIEQYGAA
                     RGLWLAIKRLSRCHPLHPGGYDPVDEHEKEAKE"
     gene            38977..39648
                     /locus_tag="VFC74_03820"
     CDS             38977..39648
                     /locus_tag="VFC74_03820"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HZK24495.1"
                     /translation="MIAAIVDVLNSILLAINDFTHNYGLSIIVLTILVRLVMWPLMSK
                     QLSSAKAMQEIQPELKKIQEKYKNDKEKLNQATMELWQKHKINPAAGCLPLIVQMPIL
                     FAMFRMLQSAPTIDPQQYFLLGIDMRVALQSTVNGAAVWQQYPGYWILVILSGLTTYI
                     QQKLTMTDPSQKMMMTIMPLMLLYFSLRFPAGLVLYWVVNNLLSLVQHLLIHRQPPKG
                     AVNEQ"
     gene            39645..40265
                     /gene="jag"
                     /locus_tag="VFC74_03825"
     CDS             39645..40265
                     /gene="jag"
                     /locus_tag="VFC74_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HZK24496.1"
                     /translation="MKVVEATAKTVEEATQKALAEIALPTERVKVEVVTEPSAGFLGL
                     IGSKQARVRVTETISPAQYMYDFLEQIIGKIGLTSTIEVTETAQVLKLSVNGSKMGIL
                     IGKRGQTLNALQYLLSVVYHKKFPGQDGRLLLDVEDYREKREETLRTLAQNLAKKALR
                     SQREVVLEPMSPQERRIIHTELQEHAAVSTYSEGDEPYRKVIIAPK"
     gene            40372..41757
                     /gene="mnmE"
                     /locus_tag="VFC74_03830"
     CDS             40372..41757
                     /gene="mnmE"
                     /locus_tag="VFC74_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HZK24497.1"
                     /translation="MFEDTIAAISTPPGEGGIGIVRISGPQAEQIGRSIFSFAKQPEN
                     LLSHHLYYGKVVNPLKEQVIDEALISFMRAPHTFTREDVVEVNCHGGIMPLSKTLALI
                     LAQGARLAEPGEFTQRAFLNGRIDLAQAEAVIQIIRAKTEAAMLVSVRQLTGHLSQEV
                     KQIRRRLLAMLAHVEASIDFPEHQDVEDLAREEIANSTQAAFAQLEALLATAEQGRIL
                     REGIKTAIVGLPNVGKSSLLNALLREQRAIVTEIPGTTRDVLEESINLGGIALTIVDT
                     AGIRETRDEIEKIGVQLSRSAIDKADLVLLVLELTAGLNEENKLILQIIKDKPCIIIA
                     NKTDLAAEPQAAAAQLKAQLTPLPVVPMSVLKQQGLQDLEDTIIQYVFKGAVQTPDSV
                     LVTSTRHKNALLKAKNALYDLQNALEAGYAVDLLAIDLHAALDALGEITGESVGPELV
                     DEIFSSFCIGK"
     gene            41776..43671
                     /gene="mnmG"
                     /locus_tag="VFC74_03835"
     CDS             41776..43671
                     /gene="mnmG"
                     /locus_tag="VFC74_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HZK24498.1"
                     /translation="MTYVGGEYDVIVVGAGHAGCEAALASARLGCKTLLLTINLDAIA
                     LMPCNPAIGGPAKGHLVREIDALGGQMAHNIDQTSIQMRLLNTGKGPAVHALRAQADK
                     KMYMWLMRKTLENTANLDIHQAMVERFQVRSGQVQGVITGTGALYRAAAVVLTTGTYL
                     RGRVIIGDYHYPGGPNGQLASTKLSTYLKQIGLELRRFKTGTPARVHRDSIDFSKTIM
                     QPGDEQHYAFSFTGPPAATPQIPCWLTHTTEKTHQIIHANLHRSPLYAGDIKGVGPRY
                     CPSIEDKVVRFKERPTHQVFLEPEGADTAEYYVQGMSSSLPEDVQVAMLRSIPGLEKV
                     KVLRTGYAIEYDCLDPLQLKSTLETKTIAGLYSAGQINGSSGYEEAAAQGLVAGINAA
                     LAVQGKAPLLIDRARGYTGVLIDDLVTKGTLEPYRMMTSRAEYRLLLRQDNADARLTP
                     LGYEIGLISPERYQSFTAKSSAVAAEKERLQKITITASEQTNELLQAWGSRPLTTAVK
                     ASQLLKRPEISYQNLLQLLPEAALLSTEAAQQVEIQVKYEGYITKQEQQIEKFKRMEH
                     KVLPENMDYLNLSGLSHEAAEKLKQIKPHSLGQASRISGVSPADIAVLLVHLQQHKKR
                     GGQGHVE"
     gene            43661..44368
                     /gene="rsmG"
                     /locus_tag="VFC74_03840"
     CDS             43661..44368
                     /gene="rsmG"
                     /locus_tag="VFC74_03840"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009268932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA];
                     GO_process: GO:0046118 - 7-methylguanosine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HZK24499.1"
                     /translation="MWNELSHLSTDLGISLPETAPSLFRRYYELLIYHNKQINLTRIT
                     EAQEVMIKHFLDSLMLLVIFPQLSGPLLDLGSGAGLPGIPLKLARPSLELTLLDALQK
                     RINFLQQLSENLQLTGVNYLHGRAEDYGQQIAYREKFPLVVSRAVAKLNVLLELCLPF
                     VAVGGFFVAYKGPEGEKELQLAEHALLALDGEPVKVWTYDLPRQFGKRSLLVIRKNSR
                     TSARYPRKAGLPAKRPL"
     gene            44555..45463
                     /gene="noc"
                     /locus_tag="VFC74_03845"
     CDS             44555..45463
                     /gene="noc"
                     /locus_tag="VFC74_03845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014315560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0032466 - negative regulation of
                     cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HZK24500.1"
                     /translation="MSEDWGSLMNIEEMEYEQGELQKIDINKIRPNPFQPRKFFAAVK
                     LAELMQSIKTYGLLQPVIVRTISGGYELVAGERRLRACQQLGWTQIPAVVKDISESAM
                     ATMALIENLQREDLSFLEEAAGYERLLREFKLTQEVLAQRLGKSQSTIANKLRLLKLP
                     DSVKAKLRNHDLTERHARALLKLPDEEAQNKILHEVCNLGYTVRQTENRVAAYLSKLA
                     PPSKERKKVIIRDVRIFLNTIRQAVSLLESGGLGPRLKEKDCGDYLEVTIYVPKSKRT
                     SKLAEQYKKETESAISQVIAHEAANN"
     gene            45567..46343
                     /locus_tag="VFC74_03850"
     CDS             45567..46343
                     /locus_tag="VFC74_03850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK24501.1"
                     /translation="MTRIIAVANQKGGVGKTTTSVNLSACLAAEGKKVLLLDIDPQGN
                     ATSGIGLEKRELKFCIYDSLINEVPLQTVIQRNVLPNLDVAPATIQLAGAEIELVPTM
                     SREVKLRRVLSEIKELYEFIIIDCPPSLGLLTINALSAANSLLVPIQCEYYALEGLGQ
                     LTNTVKMVKKHLNTELEYEGVLLTMFDARTNLSVQVADEVKKFFGSQVFAAVIPRNVR
                     LSEAPSHGMPIIAYDERSKGAETYIQLAKEVIANGQKKAG"
     gene            46321..47163
                     /locus_tag="VFC74_03855"
     CDS             46321..47163
                     /locus_tag="VFC74_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009263541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HZK24502.1"
                     /translation="MGKKRLGKGLQALIPEIDETPLSDAIEIGVAEIEINPNQPRKSF
                     DEEKLLELTRSIEQHGILQPLIVRPTGSTYELVAGERRLRAAKKAGLDQVPVLIRSLS
                     DREMMEIALIENLQREDLNPLEEAEAYKRLMEEFSYTQGQLAERVGKSRSAVANTLRL
                     IALHHDVRREIVTGNLTEGHARAILGLPLEKQPEAARKTIELGLSVRETERLAGQVQK
                     KTSVRQQKTRKTKDGYIVDIEERLREKYGTAVNVRISNKGGGKIEVHFYDLEELERIC
                     ETLF"
     gene            47364..48077
                     /locus_tag="VFC74_03860"
     CDS             47364..48077
                     /locus_tag="VFC74_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HZK24503.1"
                     /translation="MFLILQGTIVNGIAIVVGGLLGVCLGHKMPERIKELIMQGISLA
                     VLVIGMQMALQMQNAVLIIFSLIIGGVLGELIGIDEWLQRVGSWLEQRAARASSGLAR
                     AFVFATLVYVVGAMAVTGALESGLLGQHQTLYVKAILDGFTAIAFAATMGPGVCLAAI
                     PVVLYQGGIALAAGSLQPYLSPAVLLELTGVGGILIIAIAFNLLALKEIKVANFLPAF
                     LVVLLWLIVLLPLYANFSV"
     gene            48098..48586
                     /locus_tag="VFC74_03865"
     CDS             48098..48586
                     /locus_tag="VFC74_03865"
                     /inference="COORDINATES: protein motif:HMM:NF025935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HZK24504.1"
                     /translation="MELYLYLLAGGSLLLLVFCSMLAVRLKRLTQRYQEFMSGTDSVS
                     LEGMLVNLHADLRSVAAEIKENRQQMSLLQHNFVSTVRGIGVVRFNAFQNTGGDLSFA
                     VALLNGKQDGVIFSSIYGREESRFYAKPVTAAQSTYKLSAEEQEAVRQATAMLVTQAE
                     KL"
     gene            48601..49506
                     /locus_tag="VFC74_03870"
     CDS             48601..49506
                     /locus_tag="VFC74_03870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK24505.1"
                     /translation="MHNEKRYFFIINPTAKNGQAVAVWQQLQLFLDLQRVSFQFAMSK
                     DIADVEKLAKAAAAAGAIVVGVGGDGTLSRIAGALAGTKTTFGLIPAGTGNDFARTYQ
                     IPDRPEAAMQIILNGYTIALDLGMVNGRYFYNAVGSGLDAEVVADANLTFKRIGGSFG
                     YLLALLRQLILYRPQKIKITVNDEVHVVEAWLVTVANACYFGSGMKIAPCANPQDGLL
                     DVIVVQGIHRLNFLRLFPRVYRGQHIKHPAVRVWQGTKIAVESEKPLAVQVDGDLCGQ
                     TPLLVTMQAAMVQLLVPDQHKTSCS"
     gene            49539..49778
                     /locus_tag="VFC74_03875"
     CDS             49539..49778
                     /locus_tag="VFC74_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuS family protein"
                     /protein_id="HZK24506.1"
                     /translation="MERTIAVEEGLTPVLKYLQEKGYHVVSLQSGQNADAAVISGGDK
                     DVMGMQTIVQDGPVIDARGMHPEDVLRAIEGKWRH"
     gene            complement(49824..50444)
                     /gene="yyaC"
                     /locus_tag="VFC74_03880"
     CDS             complement(49824..50444)
                     /gene="yyaC"
                     /locus_tag="VFC74_03880"
                     /inference="COORDINATES: protein motif:HMM:NF018564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore protease YyaC"
                     /protein_id="HZK24507.1"
                     /translation="MSTYPPHSKQNKQSVYRVWHTDLFPAQEIKLALQSLIKEDFCPD
                     QQHLVVLCIGTDRSTGDALGPLTGTQLTRMQTAPTAAVYGTLAAPVHAVNLAETIITI
                     SQRFADPYIIAIDACLGKNESVGAIDIGRGSLRPGAGVSKELPAVGNIYINGIVNVAG
                     CLEYYVLQNTRLNLVFNLAESIAQGISLLLPELPEIVTVSTNEAAR"
     gene            50577..51209
                     /locus_tag="VFC74_03885"
     CDS             50577..51209
                     /locus_tag="VFC74_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24508.1"
                     /translation="MSWVDLAALSIMLWGAAQGYLNGPLTAIIRLLGSILAVLMAFFL
                     VNPLAVYVQNEWQLEAIFVDWFQRTGKAAILVTQAGEQYLNLPTAAAPLLRKLVPELA
                     ASPVSGAQEALVPLLAALAVRILILSAALIFILAVIRVFTQIKAAAASSNQPEWQRLL
                     GLVVGSADGLFFALLTCVALDAFSLLVYPTLIQGDLTSSYLWRAANYLIN"
     gene            51354..52004
                     /locus_tag="VFC74_03890"
     CDS             51354..52004
                     /locus_tag="VFC74_03890"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HZK24509.1"
                     /translation="MRIVLLGPPGAGKGTQGERLVKKYNMPHISTGDIFRAAIKEGTE
                     LGLQAKEYMDQGQLVPDELVVDIVKARLTQPDTNNGYLLDGFPRTVAQAEALDANLQE
                     LSTPLTGVLNVDVAEEELIERLTGRRVCRACGSTYHIKFNPSTVRSVCDKCGGELYQR
                     ADDTVETVKQRLTVYRQQTAPLINYYREQGILFTVDGSREIEIVFQEITLLLDQKQ"
     gene            52032..52922
                     /locus_tag="VFC74_03895"
     CDS             52032..52922
                     /locus_tag="VFC74_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HZK24510.1"
                     /translation="MLQYLSQLNISRTAIEDYFIDISLTWGSRIFKGILIILLARLAL
                     RFGQNVIARLLTPGQHKPSILPEGKAKTLLGLLFSLWHYIVYSFAILLLLSNLEINIG
                     PILAGAGIVGLAVGFGAQNLVRDVISGFFLILEDQFTIGEYVTVLEYSGTVEEIGLRI
                     TKIRGWDGELYIIPNGLIASVTNWNRGHMRALVEVGVAYEEDLDRVLTLLEQVAVEYA
                     ATDADVVAGPSVVGVMALADSSVVIRVVATTISMSQWKVERNLRLAIKRKFDEEGIEI
                     PYPRRVLEYRSKLKGEDSRG"
     gene            52915..53091
                     /locus_tag="VFC74_03900"
     CDS             52915..53091
                     /locus_tag="VFC74_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HZK24511.1"
                     /translation="MADFTVGEKVRMKKVHPCGGDIWTIVRVGMDFRIKCINCGRSIM
                     LPRSKFEKNVRERL"
     gene            53201..53491
                     /gene="rpsF"
                     /locus_tag="VFC74_03905"
     CDS             53201..53491
                     /gene="rpsF"
                     /locus_tag="VFC74_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HZK24512.1"
                     /translation="MTKYETMFILRPEQDDDTYNTMVEKFTGIIEAEGGEITNINRMG
                     RRKLAYEVKKQFREGYYVLINYTAEPSVTNELERNFKISDDVIRYLIVREED"
     gene            53512..53898
                     /gene="ssb"
                     /locus_tag="VFC74_03910"
     CDS             53512..53898
                     /gene="ssb"
                     /locus_tag="VFC74_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003495585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK24513.1"
                     /translation="MLNRIILIGRLTRDPELRYTSTNGVAVASFTLAVDRRFTQGQQR
                     EADFIPIVTWRNQAENCAKYLGKGSLVAVEGRLQIRSYEKDGQRRTAAEVVADSVQFL
                     DKREKRSSDDAPSFDDAMIGDDDVPF"
     gene            53958..54188
                     /gene="rpsR"
                     /locus_tag="VFC74_03915"
     CDS             53958..54188
                     /gene="rpsR"
                     /locus_tag="VFC74_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HZK24514.1"
                     /translation="MRRDRGRRGRRKVCNFCVDKVEHIDYKVTNKLARFLTERGKILP
                     RRISGNCAKHQRQLTNAIKRARNIALLPYTAE"
     gene            54482..54934
                     /gene="rplI"
                     /locus_tag="VFC74_03920"
     CDS             54482..54934
                     /gene="rplI"
                     /locus_tag="VFC74_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HZK24515.1"
                     /translation="MKVILAQDVKALGKKGDVKEVAEGYARNYLFPRGLAVEATGGQL
                     KELQQQQQRTAEKQAKVLAEAERKAAKISGMQIEISMRVGENGRLFGSVTSGDLAVAL
                     QKKGVKVDKRKIELSEPIKNLGSYPLRIKLHSEVDASLTVKVVAAAPK"
     gene            54951..56873
                     /gene="lonC"
                     /locus_tag="VFC74_03925"
     CDS             54951..56873
                     /gene="lonC"
                     /locus_tag="VFC74_03925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012034021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lon family ATP-dependent protease"
                     /protein_id="HZK24516.1"
                     /translation="MKKKLTTRSIETNDFSAEPEQQKKEINALYALLSQLLGADRLIL
                     KAGKLESLSLLRSRQPAERLLGLQRVILENPTLSEVPTAAQIPLILQQLYDELADNMA
                     RRTVEENLERKVAEKMQEKQDEYLHEMKRQLLKDEGGPENPQTLKKYAQLEKMKHTKL
                     NRSALEILRPHTLTEIVGQEAAVTSLMAKLASPYPQHIIIYGPPGVGKTTAARLALEA
                     AKQLADSAFSPQAPFIEVDGSTLRWDPRETTNPLLGSVHDPIYQGAKRDLVDGGVPEP
                     KPGLVSDAHGGILFIDEIGELDLQLQSKLLKVLEDKKVSFDSAYYDPTDAQVPQYIRA
                     LFEEGAPADFVLIGATTCAPEEINPALRSRCTPVFFAPLTPQDIESIVVEAAAKLSVT
                     LANDVAALIASHTTEGRQAVNLLADAFGLTRYRQQQQTEAVISKELVLETLRAGRYSP
                     QTSKKYTGGEHLGRTYGLGVSGFLGSVIEIEAVAFPANIAGKGGIRFNETAGSMARDS
                     VFNAAAVIRQLTGEDLQNYDLHVNVIGGGRIDGPSAGLAIVLALLSAIKGWPLSQEVA
                     VTGEISLQGYVRPVGGIPEKLYGARLAGINTVVVPYENKDDIPSLPGMQVIPVKKIED
                     ALPLFFKGQEEVISHH"
     gene            56906..58243
                     /gene="dnaB"
                     /locus_tag="VFC74_03930"
     CDS             56906..58243
                     /gene="dnaB"
                     /locus_tag="VFC74_03930"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HZK24517.1"
                     /translation="MMERVPPQNLEAEQSVLGSMLIDRDAIVAAAELLKPTDFYRGGH
                     QNIFAAMLALSEKNEPVDLITLAEALSQQNQLEAIGGVAYLTTLANVMPTAANIGYYA
                     KIVQEKAVLRTMIHAATGIVSRCFEANDSLEEIVDEAEKTIFEISQRSTPQGFANMKD
                     ILKGAFDRIDKLWGNKGGVTGVPTGFHDLDNITCGLQNADLVIIAARPSMGKTTLALN
                     MAQHIAVNEKLPVAIFSLEMSKEQLVQRLLCAQANIDAQRLRRGYLSDEDYPKLTRAA
                     GPLSEAPLFIDDTAALSVMEMRAKARRLKAEHGLSAIFVDYLQLMRGSGRVENRQQEI
                     SGISRSLKALAKELDVPVVALSQLSRAVEQREKKRPILSDLLESGGIEANADLVAFIY
                     REGYYDQNMENRHDTEIIIAKQRNGPVGSVHLYFKENHNKFLNITTEQVEQVS"
     gene            58324..58399
                     /locus_tag="VFC74_03935"
     tRNA            58324..58399
                     /locus_tag="VFC74_03935"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58357..58359,aa:Lys,seq:ttt)
     gene            58404..58478
                     /locus_tag="VFC74_03940"
     tRNA            58404..58478
                     /locus_tag="VFC74_03940"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:58437..58439,aa:Glu,seq:ttc)
BASE COUNT        16240 a        13334 c        12539 g        16390 t
ORIGIN      
        1 atgaaaatga tttaccattt actaatataa taggtaaagt atatgattct tcagtaaaaa
       61 atataatagt tcacagtgat ggtaatgatc aaattataaa tgttaattct aatcgacttt
      121 tttactataa atcgaatgtt ccattaagat ttcttagttt tactacaaat tgacttattc
      181 agtagaatta gtccagctct ttaattatct gtagcctcct ttggtgctaa gtctactcat
      241 aggtctggct ttaattatgc ttttacaccc ttggctatgg agatggcaca tacgaagtct
      301 ttgcaaccta tgatgatgac ggcaccagtg ttaaggtaga aatagaacta gcctaaaaag
      361 caaaagctct agctagaaca agcacctagt tagagctttt tgttatgcgg ttttcctgac
      421 ttcttgcctt ctacctgcta caattaagat acagcaatta caaagaagaa cccggaagga
      481 gaaccgccat gagaaatatt tatctgctgt attcatgtaa cgaatggaaa agctgggata
      541 gtatacgttt aattatggcg acaacttttg agacactccc ttacctccta ccctatcgag
      601 caatctgata tgatatccac gaatctgtca acacctcggc ctgtttcttc tccgtctcta
      661 ttgccaactt gctttctcct ctcgaagtca acgagatata cttccttact aatcatctct
      721 cggctcttcg atttcgaacc cacccttaag aatgaactcc tgaagcaaat tgtgtacttt
      781 aatggtgatc taagatgcgc tatacatgca gcgtattagg ccgagaagtc tatctgttct
      841 ataacggttt tcgctggttt atggcacggc ggcaggtgta agacaagctt tggtctctaa
      901 ccagagcttg tttttgttta ttcatcacac tggttttcta caatttcttg gggatctaac
      961 catagagcat caggctttgc aataactcgt gcgttttgat aagcggcatc aagcggcaga
     1021 atcgtttccg gtgtatagat attcttttct aaatctaata ctagtgccag gtcgggcgat
     1081 ttcgtaaatt caccgccaag gtataacggc agcagaaact gaattttatc acgttgaggc
     1141 cgatactggg gtattactag cttataattt ctttcggcca tcgcgatacc ataatcgatt
     1201 gcgctatcca gtaatcttgc tagtgaggta acatcaaaat gttcgaattg ttcaggaaac
     1261 ctttctaggc gctctataat tatatgttca aacttgatca aagttacttg acagtaacag
     1321 gtataaaggt gaattaatat acacaaaaag caagttctaa cctgttgttc ggttaaaact
     1381 tgctttttgt gcgcttttcc tgaccctctc cccccttctt gctacaatta agatagaaac
     1441 aaagggagga aaaaaaaagc gttgtcgtgt tgactcggca cagtagcgct tattgatccc
     1501 tgctacattg acgacaatca atgggataaa aagtaattga taattctacg ctggctgtct
     1561 caaggggtgg gaagttatct attgcctccc acactgcttg agcaggcaat agggtaattt
     1621 tttactagtg cagcaaaaaa tacaaaacca ataaatgacc aggataaaag ccatacgcca
     1681 gccaagtagg cagaatcttc ttatccctga tcttaaaaag gggataggct aaaagagcaa
     1741 atccctgaaa gccagaaaag atgttcactg caaaatatat gatggtgtac tttatgtcct
     1801 gcaagtcaat cttatctaca agaaaaaccg ttagtggacc ataatacccg tactcaaagg
     1861 agaacatgac tgttagcgca aataggccgg cagccgcaag ataaatcttg ttatcaagag
     1921 ctataagcat caacagagag acaaacagcg taaacaatat attgagatca gtagtatcaa
     1981 aaaacagcat ataaggaact tgcgagagaa tcgccagcac aagcagccta gtgagatatt
     2041 tttttatact tctggtcctc cttctccccc tggccagttc tagtgcaaac aaagggaaag
     2101 caatgcgccc aatgaggcgt agaatcgcca tctcagggaa aaaggcaaaa cctatatgat
     2161 ctatgagcat cgttgtgatt gctatcagtt taatcatagc tttcctcact aattctaatt
     2221 attcactggt atttactatt tgtggtataa gcgaaatttg tttcagagta gttttggatg
     2281 attcaatctt taagacatcg cataatggcc atgcagattg tagccgcatt tataagttat
     2341 gttcatcaac ttaacaataa attaaacaag aagtcatctc cttggaaatc attgttatat
     2401 aaagagtggg atactactac ttctgtagtt ccttcacgac gagggaagcc aaacgactaa
     2461 tcgttctggc tgcggcacgc cgtgcgaggg cgttggctat tgccaggcga cagggtggaa
     2521 ttgttaccat gattgtcaaa gccaccgggg ggcggctggg aaaataaatc aaagccatgt
     2581 ctccaaccat atgatcaaca gttcccactt tattggccac caaaacgctg ttgcttttca
     2641 aatagcgacc aattcggttt ttgttttcag tgtggagcat catcgaaaca gccagatcct
     2701 tcaatttttc accacaaacc tctctgtttt ctagcgcaat taacagctcg gtcagctcac
     2761 gtgcagtaga ggagttgggg ggtaatggag aatccaaggc atacattttt ctggtgccac
     2821 gagtctgcga caggcccagg cgcgtggtca tcgcggccac agcttgggga ccgactaaat
     2881 caagcacgat attagcggcg acattgtcgc tggcgctgat catcatcttc agcagctctt
     2941 ctactgtata gatacctccc gcttcggtaa actgcaggct gccagcgcca gttacctttg
     3001 tacttcctgc atatttatcc tgctcactaa aggtataact gtcgttaagc gctaatacac
     3061 cttgctctac ctggtctaag acggttagaa gataaaggat ttttattaag ctggcgggat
     3121 agagtctgtt gtctgcatcc ttttctatcc actgctttgt caggaaatca tataaacaga
     3181 ggctggtctg gaggcctctg ttttttgctt ttcttgctag cagcatgtta agtttattaa
     3241 aattttgtct agctttgtcc agttcttcgt gcttcacaac ttttctcctt ttgaggatag
     3301 ttacccttaa ctctattgta atcgattttt ccctttttct gcaagaccgc accttctact
     3361 accgtaaata caagcagtag gttgtcagat aatagcaggc ggtgcatttg ggggtaagaa
     3421 aaaaaggacc atcatggtcc ttttggtttt agtatggcgg agagacaggg attcgaaccc
     3481 tggaagcggg ttttagtccc gcttaatcgc ttagcaggcg accgccttcg accgactcgg
     3541 ccatctctcc gtgtgttatt aacttaactc cgccagctta ataaatggcg gagggggtgg
     3601 gattcgaacc cacggctctt tcgagtcact ggttttcaag accagctcct taaaccgctc
     3661 ggacacccct cccgggcgca taaattagta taacataggc ctatatggat gtcaacaaca
     3721 ggtgagctgt tgttatttac caatataacc gctctttatc tggggcagat aggggcataa
     3781 agcttccggg attgcaacag taccgtctgc ttgctggtaa ttctccaaaa tagctgccac
     3841 tgttctacca atggctaccc cagagccatt taaagtgtgg acaaactcag ctttaccgcc
     3901 ttgcaggcgg cggaaacgaa taccagcacg acgagcttga aagtcaccaa aattgctgca
     3961 ggaagagatc tcacggtagg tattatagga cggcagccag acttccaagt catatttttt
     4021 agccgccgca aaaccgagat ctgcggtaca catgaggacg gttcgatacg gcaggcctaa
     4081 acgctttaag acttcctctg cattgactgt caacttctcc agttcagtca tggattcttc
     4141 aggacgcaca aattttacta attctacttt attaaattgg tgctgacgga ttaagccacg
     4201 tgtgtcacgg ccatgggcac cagcttcggc gcgaaagcag gcactgtaag ctacatagca
     4261 gcgcggcagc tctgctgctg taaaaatctc atcacgatgc aagttagtga ctggtacttc
     4321 tgcagtagga attaaaaaat aatcggtatc tgctactcgg aaagcttctt cttcaaattt
     4381 aggcagctgc cctgtgccta ccatactgtc acggtgcacc ataaaggggg gaaatatttc
     4441 cgtataacca tgttctcctg tatgcaaatc tagcataaag ttaaccaaag cacgctcaag
     4501 ctgtgctccc cagcctttgt aaaaagcaaa gcgggcacct gtcactttcc cggcacgttc
     4561 aaaatcaata atatctaaat tagtggcaat atcccagtgt ggctgcgctg taaaattaaa
     4621 gttaggcaca tcacctacat ggcgtattgg tatattatca tcctcactct gtcccacagg
     4681 aacagaagcg tcagggatat tgggcaaggt taacagaatt tcgtgtaaat ccttttctgc
     4741 ttctctggtt tgggaatcca attctttaat gcgctgcgct acgccacgca tctcttcaat
     4801 caccgtacta gcatcctctt tagccttttt catgcgagct acttcagctg aaacaagatt
     4861 acgcttattt tttaattgtt ctacctcttg gagtaagtca cggcgtctgc catcaacttc
     4921 attaaagcgg tcaatcgcag ctgtttctcc cttatcatgc aacgcctggc ggacaatatc
     4981 caagttatca cgcacaaatt ttaaatcaag cactttgtca tcctcccttt tcaaagtgtt
     5041 tatgtaataa aaaagcttac gctttcaact tccagagggc gttaaaaata aaaaaaccca
     5101 ccccccaaca gggacgagtt gcttcgcggt accaccctgg ttgaccaaat aatttggccc
     5161 cctctatgcg taacgtgcaa aacggaatag attactgtga gttcatctat tcacctccgg
     5221 gccggaattc acccgccgtc ttaccggttc ccagcacctc cggctctctg taaaggacgg
     5281 taatggctac ttttgccctt ccttggtttt attcctttag tattcatagt atacccttct
     5341 cactataata atgcaagtat attatgccgt gtggtatact aaaaatacca tgttctctgt
     5401 aatgtttagt gggtataatt ttcttaataa ataagctcaa tgatgggatg tgaaattttt
     5461 gcaagcattc ttaatcgcct ttgccgcaac tttgttagcc gaaatgggtg acaaaacaca
     5521 actagttaca ctgactctat caactcgata tcccccctta cgggtgctgg ccggttcttt
     5581 aagcgcctta gccgccatta cggggcttgc tgtgctctta ggggaatatt tggctcaact
     5641 ctttccagaa atggtcacga tgctcgctag tggtcttttc tttattgttg ttggtatctt
     5701 cttgctgctg aaaaaagaag agaaagaaga tgctgatgtg cgcacctccg gtcgtggtat
     5761 tactttacaa accttctttt tagttttttt agcggaacta ggtgacaaaa cacaacttac
     5821 tgcaattgcc ttgacagcag cttacggctc acctttagcc gtgttcctgg gcgccatggc
     5881 cgggcagact gttaatcata gtctggcagc ctacctcgga gcacgttttc tctcccgctt
     5941 acctgcacgg accattaaaa taggcagtgc tttactcttt attatctttg gcctcatctt
     6001 tttgtatact gctttacgct aattagaaga attgattact ctgaggccac ttagcgcctg
     6061 ggaaagagaa attaatttgc caggaaaata aaagctcaac cattgcggtt gagcttttgt
     6121 ttgtgaccac tagttcacaa gcaagacctg cctgacatat tatatttttt tgctggtttt
     6181 tagctaagca cgctacattt cttcaggggc ggcgataccc aataggtata agccgttgac
     6241 taaaacttga cgcaccgcat caatcagggc taaccgggca gtacgcagtt cttcttctgc
     6301 gtttaaaacc gggcattgat ggtagaaacg attaaattct gccgctaaat ctaccacata
     6361 acgagcaaca atggaaggtt tgtacgtctt agccgcgcgt gtgatagtat cagtgaaacg
     6421 tgccaggaga ccaattactg cttgttcttc atctgttgtc agcaaagccg catcatagtc
     6481 cgctggccac gctggtgctt tgcgtaagat actgcagata cgggcatgcg catactgaac
     6541 atagggagct gtttcgccag aaaaatccag caccttgtcc caatcaaact caatattttt
     6601 aatacggtca ttactcaagt caccaaagat tacagcaccg atccctacct ggcgtgccac
     6661 ttcggcttta ttagccagac tggggttctt atcctgaata atttgggctg ctaattcggt
     6721 agcccgctgc agcacctctt ccagaaaaat tagtgtacct tgacgtgtgg acattttccc
     6781 gtctttatag ccgatgagac caaacgggac gtgaaaacac tgctcggccc agctatagcc
     6841 cattttacgc aagacagtaa aaaattgttg gaaatggagt gcttggtctg cccctaccac
     6901 atagagcatc ttctcgaaat tatattgctg ccaacgataa atggcggcac agagatcacg
     6961 ggtagcgtat aaagtagcac catcctgctt acgcagcaaa catggcggca tgccttctgc
     7021 acttaagtct actaccagtg ctccctcgga ttcactagat aaaccttgct ctgccactaa
     7081 tttaatcgta tcatccaaca tttcattata aaaactttcg ccggaataag aatcaaattc
     7141 tacatctaga atctgataaa tacgtttaaa ttcgattaaa cttaaagcac gaaaacgctg
     7201 ccacaaatct tgcgcttgcg gctcaccgtc ttctagtttt ttaaaccagc tacgtgctgc
     7261 ttcgtttaat tgcggctcgt gttcggcttc ctggtgaaaa cgcacgtaca attcatataa
     7321 atatgtaatc gggtcttttt gcagctgctt ttcattgccc cattttaaat aggccacaat
     7381 gagcttgcca aactgagtgc cccagtcgcc aaggtgatta ataccgatac agcggtagcc
     7441 tagggcttcg taaaggcgat acagagaatt accaattact gtagaacgta agtgtccaac
     7501 cccaaagggc ttagcaatgt tgggcgcaga aaaatctatc acaattgttt tgccttgacc
     7561 agcttgctgg tgaccatatg cttttttttc tgataatact ttctccaccg ttaagcaggc
     7621 cagcttttgc cggtcgcaaa aaaagttaag ataggggcct ttagcttcaa tgcgctcgaa
     7681 taatgcgctg gcgggtagtg cttcaaccag tgtaacagct attttaggtg gagcctgccg
     7741 cattgcttta gctaacttaa aacagggaaa agcaaagtca cccatcgcag ggttgggtgg
     7801 cgtttctaaa tcttcgctaa tttgctgctt atttagtggc acatgaggtg ataataaacg
     7861 aataatttct tcccgaaacg tctgcatcaa acaaggacct ctttcttcaa ttgttgacat
     7921 tattatatcc aaagacaggg cagactgcaa gcttgtcctt tttcgctcag gacagcaaaa
     7981 tgcgacgcat aggtgcgccg cattaactag ctcgctttag ttttccatat tcacgtaata
     8041 gggacggata acagttactt cccaaggtaa ataatgtttc tcacttttag gtgtggtacc
     8101 attaaccgta atcttcactg tagaataagg taagttctct actaatgaca atgctagggc
     8161 gtcacgcagc attgctgccc gggcagcggc cgcgattgat tgagaaacaa ggtcatgtaa
     8221 ggaaggggtt aaatcaacat agataatatt gtcagcaatc tttactgatt gcagggtaag
     8281 caccttttca aaacaatctt cgtaacctgt taaaactgtt tgcaccactg cttctgcaag
     8341 ggctcgctcc ttaagcggcg taaattcttt ggtgacaggt cttaagtaat attttcttcc
     8401 agaagcacta ggtaaaaaga gtagacgttc tccacttttt ttactaaacg gctgcggctg
     8461 aaaaggctct tcaattaaga gaccttcctg gccaaaagtt attctctctt ctccagctac
     8521 cgtaaattgt atctgttcca ccgagggaat actgctgaca gtcaagagta aggaatctaa
     8581 aacacccgtc aaaaaggtac tgcctgacat ttggttcaat tcttctggca aatccacttc
     8641 tacggtgccg ttctctacct ctaccttaat ctctgactct ttgggaatag tgcgctgcaa
     8701 ttcactgccc tgtgcaggcc cccgcactaa ctccaagatg ctaccacgca acagtgattg
     8761 tgagcttttt acactacggg tcaccggaat tagatagcgt gagtcttgat catagaagta
     8821 aagggtaaca ttattttctc cttcttgacg tccaaaaaca actggttgat aataatctgt
     8881 tgtttttggc tctttggtcg tgtcagtccc cggcgctttt ccgtaaagat ctaaaaaata
     8941 aatactgcca tcataaagac ctacaacgag tgtacctgac tgtgaggcaa aagccatttt
     9001 ttgtgcccgg ccggtcacgt ctagaacgtt gatttgttgt ccctgttgat cgtactcagt
     9061 aattttagtg acatcatctt gaaattgtcc ccaagaagcc acaattacct gactgccatc
     9121 agctgaaacc acgacattag ccccgaccgg cagtacttgc tgccacagct tttcgccagt
     9181 catactaaaa taaagcaaat tctgatgctg tgttgccggg tcggtaataa ttacaccgac
     9241 atacaagcca ttatcactga cctgtacacg actaacttca ccagataccg catattccca
     9301 gactttttct gctttgtaat cgtaacggga tatttctgca gagaatccac taataatgtt
     9361 ctggccacta ctgtcaacgc taatggcagt accctcctgc agtttccaca acaactctcc
     9421 ttcagcagag aaaacaccaa aataggggat ctcaccttcg cgccaattga ccacaacacg
     9481 tttatctatc ccggtcactg ctgccgagat aattgtcccc agctcttcct gccaaagcac
     9541 gttacagctt ttctccatca ctactaattg atcttgctgc tcttcatgat cagtgtgcag
     9601 tacagcagcg atcaggctgc catccttggc gatagacaac tgtgtcaaag gtgcatcaaa
     9661 ctgtcggctc gccttttcct tttgatcagg accgatcagt gtcagttggc caccttctgt
     9721 acccaaggca ataaaactac cgtctggggc aatatatgtc tgggtgggta tactttgcag
     9781 ctgtctttcc caaacaggct taccttcttt tttcagcagg taaacaactt tagaggctgt
     9841 acccacaaca gtgaataagc catctgctgt cacagaaatg tctgtaatct gcaattcact
     9901 gtttttaaca ttgaattgac ccacggccgg ggatcttact tcctctgccc ctgctacaat
     9961 tggctcaata ggtttaccgt tattcgtaag ttctgaaatg caacccgtta ataacagtgt
    10021 cagcagcaaa atgggcgcta ttattagctt ctttgtgtat tttttcatca tacacctcag
    10081 tttgtatgtc tcttacctca attataacgg ttaaataggg atgtttattt aagttttctt
    10141 taatttttgt taactaaacc gacggaagct taggtagtgt taccacaaaa gttgaaccac
    10201 tcccttgata gctatcctct acccatattt taccgccatg ccgcttaaca atctcataaa
    10261 caatagacaa gccaaggcct gtaccgccct gctctcgtga acgtgctttg tcaacgcgat
    10321 aaaaacgttc aaagaccttc tcccgatgtt ccgcaggaat accaacccct gtatcagaaa
    10381 caattaattt aagcgaatct gtcgcttccg tcagcttgac ccaaatctga ccatttgctg
    10441 gggtgtattt aatcgcgtta tcaaccaggt taaaaacaat ctgcttgatt tgatgcagca
    10501 cggcaaagac aggggcagcg cgcggtggca gttcgtagct aaaagcgatt tgcttgacct
    10561 gcgctctttt ttctaacatc tccaaagttg tgcgaatcgt gggcacgatg tcagtgaatg
    10621 ccatggtatc atcagctgtc acacgatcca gtctagataa atcaagtaat tcttccacta
    10681 aatgaatcag ccgttctaat tcttggtcaa tatcgcctaa aaactcttcc tgttcgtggg
    10741 gatctagcga atattcacgc attgatttaa ttaaaacgtt taaggaagtt aagggcgtac
    10801 gcatttcatg cgaggcattg gcaacaaaat cttttaactg tctggtggaa atcgctaact
    10861 gctgtgccat ctcgttaaac tggatcgtca gccgacctat ttcgtccttg gtgcgcaccg
    10921 gcacatgttg ggccaaatca ccccgggtca tctgctctgt agctagcgtc agcgctttaa
    10981 ttggcgagga gagcttctga gcaaaaaaac ggcctaggag gctggcgaca acaatggcta
    11041 gcagtgtagc agcaattaga taattgcgta tatccctttg tacgccgtgg atataggaga
    11101 gcgaggacgc tgtaaaaact gcaccattaa ctttaccctt attaatgatc ggtactgcca
    11161 cctgtagtac ccactcgccg ctcatttcag attgctgcac actccaacta tggtcctctt
    11221 cctgctctag agccgcagcg atttctggtc tttctaaatt catgcccact aaaccgtcga
    11281 cacgctgact atcacctagt acatgctgct tctggtctgt aatcaggaca cgggcgctaa
    11341 tttgccgcga gaaatcaaca gccacattag ataagtcagt aatatctggc gtattaacta
    11401 atttgggggc aacaaagccg ccaattaatt tagcagctct ggtcatcgct tcatattgat
    11461 aggcaaaata atattgctct aatgtattta aaagaaaaag tgaagttaac accagtacca
    11521 ccacgattaa aataatgtac gtgagggtta gtttggtgtg gatactatta agcatttttg
    11581 ccctcccgga atttatagcc tgccccccaa acggtgagaa ttagctgtgg ggtggcagga
    11641 tccggctcta ttttttcacg tagatgacga atatgcacat ctaccgtccg ggcatcacca
    11701 tagtaatcat aaccccaaac ttgctccaac aatttttctc tactaaaaac acggccgggg
    11761 tggctggcta gcaggctgag caaagcaaac tctttactgg ttaaatcgac atctttatcg
    11821 tgtactctaa ctttgtgctg aaaaaaatca atttgcagat cctgtaaccg gataacctgc
    11881 attcctgcag catcaaccgg ctgggcctgg gaacggcgga gaatggcttt cacccttgcc
    11941 accagttcgc gcgggttaaa aggtttagtc atataatcat cggcaccaag ctccaagccc
    12001 aaaattttat ctatatcttc accacgtgca gttaacataa taatgggcac ttcactggtt
    12061 ttacgaatat tgcggcatac ttcaaaacca tccatttccg gtaacattaa atctaggaca
    12121 attaaatcag gcgtctgctc atgaaaaagg cgcagcgctt ctttaccgtc ataagctact
    12181 cgtacttcat tatgttcttt ttccagagca aatttaagtc cttttgcaat ggttttttca
    12241 tcatccacta ccaggatgtt ggccattgga tcccctccta ttaaagtgaa cggtagatat
    12301 cacgaacttt ttgtatatca tgccacacag gacgtttttt cgtctgatcc cgcagcagtg
    12361 ctgctggatg aaaggtagcc atccaatgcc gaccatggcg ttccacccat tttcctcgca
    12421 ctacagatac ccgtgcctgt ggatcaatta aggctttggc agctactgcc cccaaacaaa
    12481 gcacaatttt ggggttaagc agttctattt gtgctaacaa atgtggtaaa caagtctcaa
    12541 tctctcctgt cgtaggggct cgattccccg gagggcgaca tttaaccaca ttggtaatat
    12601 agacttcttc gcggctaatg tcaacagaag caagaatctt ggttaatagt tttcctgcgg
    12661 ctcccacaaa aggctggcct tgttcgtcct cttctgcccc cggcccctcg cccactagca
    12721 gcagtttagc ttggggatta ccactaccaa aaatcacttg cttagctgct tggcgcagct
    12781 gacaaagctg gcagttgacc actgtctgcg ctaaagcagc taaagtggtg tctttgccct
    12841 ttgcctgcgc tagtatcacc ggtgtagaag ttacatgttc ggcctctgtt gtcgacacta
    12901 aataatcaaa taaatatttt tctctcattc ttttcacctt atgcttattt ccacaaagta
    12961 aacgttaact ccttttttac aaaaactaca tacacctact acatgcatca aacccggccc
    13021 tacggtaggg cagatcttct cgttaaagca caaagagaag gctgcccttc tctttgttag
    13081 ttttgctatt ttattaaact aaatttacca gccccgctct tgcatccgtt cctcttgttt
    13141 gatggaggag atttctaaac caggcatagc ttcgcctaaa cctttagaga cctctgccag
    13201 aaatgcggga tcatcataat gcagagttgc ttccacaatg gcccgtgcac gtgctgtggg
    13261 atctttggat ttaaaaatac cagaaccgac aaatattcca tcgcagccta gctgcatcat
    13321 caacgcagca tcagcaggag tggcaatgcc tcccgctgct aaattagcca caggtaagcg
    13381 gcccagacgt tttacctcta ctaccaaatc atagggagca ctaatctttt tggcaaaggt
    13441 cattaactct tcatccggca agttttgcaa tttccggatt tcgctcatca ctctgcgcat
    13501 atgacggaca gcttctacca cattacctgt acctggttca cctttagtac gaatcataga
    13561 ggcaccttca ccgatacgac gcaatgcttc gcctaagtta cgtgcaccac agacaaaagg
    13621 tactttaaag agttttttgt taatgtggaa ctcttcatct gctggggtca gtacttcact
    13681 ttcatcaata taatcaatac ctaatgctgc taaaatctgt gcttctgcaa aatgtccaat
    13741 ccgtgccttg gccatcacag gtatactaac tgtatctaaa atactaagaa taatatccgg
    13801 attagccatt ctggccacac caccggcggc tcgaatatct gccgggacgc gctctaatgc
    13861 catgacggca atcgctcccg ctgcttcagc tattttggcc tgttctggtg tggtgacatc
    13921 cataatgacg cccttatgca cttcagttaa gccagcgttt acatgcatcg taccttgttc
    13981 tgccattgct ataccccttt caaacgaatc tccttatgta aagagattgg attaataccg
    14041 tattttacca tagtatcttt acttggacaa gtattatttt ttattcgtct agctcaaagc
    14101 tggttttaag cgcttctttc tgttggaagc gctcttctgc aagctggatt agctgctgca
    14161 gtaattctgc gaaaggaagc ccagatgctt cccacatttt tggatacata ctgatggtcg
    14221 taaagccagg cattgtattg atttcattaa gccaaatttc ccctgtttgt tcattgacaa
    14281 acaaatcaac acgcgccaga ccagcacaat ctagagtcgt aaatgtttca actgccagct
    14341 ggcgtacctt ggtgatagtg tctggtttta gctgtgccgg aataactatt ctggaacgat
    14401 catcaatata tttagcccgg taatcgtaaa aatcattact gggaataatt tcacccggca
    14461 gcgaagcttg aggatcgtcg ttacccagga cactgcattc tatttcctgt cccgttaaaa
    14521 acttttctac gatcacacga cggtcatact gcaaagctac ctctatagca tgccgcaatt
    14581 cttgttgatt ataagctttg gagataccca cgctagagcc aaggttagca ggcttaataa
    14641 aacaggggaa acctagtttt tcttcaactg ccgccaacca gtgggcctgc tcctggtgcc
    14701 attgataagc agtaaaccaa aggtaatcac caacaggtag tccggccgcc actaaagcag
    14761 ccttcattaa tattttatcc ataccgactg ctgaaccagc cacaccggca cctacatacg
    14821 ggatgcgtac caactctaat agtccctgta ccgtaccatc ttcaccatag gtgccatgta
    14881 acgcaggaaa aatcacatca atggtcactg tattaccagc acgttcccca tcaagaatat
    14941 aaagaccatt aatatctgga tcgggcaaaa cagtcaccag actgccattc aactgccatg
    15001 aaccttcctt ggtaataaag acaggtaaaa cttcaatatc tggcagattt tttaaacctt
    15061 ccataatggc ggcagctgaa cgtaatgata cttcatgttc accagaacgt cctccataaa
    15121 gcacagctat cttctttttc atcttaacct ccatacatac ttgttaatgc agtttattcg
    15181 tcaggcaaaa aaagtatgaa acggttacga aggagcaagc gccaggcgct tgctccttca
    15241 acttttgcgc tgtttgtacg tttattcttc ttttgcggct ttagcgtcgg caatgatctg
    15301 ttcactaaga tgtgccggca cttcatcgta atggtcctct ttcatggcaa aggtgcctcg
    15361 accttgtgtc attgaccgca gatcaatagc atacttgagc atttcagaca tcggtacatg
    15421 tgtcttaatc acctgattac cattgctttg ttccatacca agtatccgac cacggcgact
    15481 gttcatatca cccataatat cgcccatgaa ctgatctggt acagttactt caacatacat
    15541 gacgggttcc aagagcaccg gctttgcttg ttccagtcct tttttaaagg cctgatgagc
    15601 agctatttta aaggccattt cggaggaatc cacactatgg taggaaccgt catacagact
    15661 agctttaatt cccaccacgg gataacctgc gacaacacca ctctccaacg attcgcgtaa
    15721 acctttttct actgctggta tatattgccg tggtacagag ccaccaaaga ttttatcaac
    15781 aaactcaaac tcctcaccat cttgcaatgc ctcaaactca atccagacgt gaccaaactg
    15841 cccacggccg ccagattgtt tcttatgttt accttctact ttagcggtac cacgaattgt
    15901 ttctttataa ggaattctag gtaaggttaa atcaacttct accccaaatt tggacgctaa
    15961 tttactggtg ataatctcga gatgcagctc ccccatcccg gaaattaatg tttgtttagt
    16021 ttctgcattg cgctccatcc ggaaagtagg atcttcctct aaaaagcggg aaaagccagc
    16081 agctacttta tcttcttcac cctgtttctt tggttctaca gcaaatgttg tcaccggctc
    16141 gggaaaagca ataggtggaa aaactgtaca taattgctta tcacataaag tgtcgcctgt
    16201 ttctgtcact tgcaattttg caacggcagc aatatcacca gccgccactt gatctacatt
    16261 aatttgattt ttaccaagca tcgtaaaaac ttggcctaaa cgttcatctt tatttttatt
    16321 ggcattatgt agttggttat caccttttaa ggtcccggtg tacactttaa aataagagat
    16381 tttccctaca aacggatcgg caaaagtttt aaatactaaa gctgaaagag ccccttgggg
    16441 atctatcttc acagtaacct tttcaccact ctcagcatct tcgcctgtaa cttcagcaat
    16501 atctgctggt gaggggaaag cggtcacgat catctcaagt aagggctgtg taccaatatt
    16561 ggcagttgca gcgccgcaca tgacaggaat aatcttgcca tttagtgtac cggtgcgtaa
    16621 acccgcatta acttcgtcat cagttaacgg ttcaccctcc aagtacttca ttaataagtc
    16681 atcgtcgctt tctgccactg cttccattaa tttttcacga tactctgctg cctcggcagc
    16741 caattcagca ggtatttcag cttcggtcat tttcttgcca tcgtcactga agctataggc
    16801 tttcattttc actaggtcca cgacaccttt aaaagctgcc tcagccccaa tgggtagttg
    16861 cacgggcacc acgtttagtc caaagaactc ctgtaactgt gcaaatactt taccaaagtt
    16921 agcattttct ttatccattc tgttaacaaa gactaaacgc ggtaatttgt tttcttcagc
    16981 aaattcccag acgttttctg taccaacttc tacgccggcg gtagcagaca gtactactac
    17041 tgccgcatca gcgacgcgca aggcactggc aacatcacca ataaaatcaa aataacctgg
    17101 cgtatctaaa aggtttattt ttacgccatt ccattccact ggggctaaag aagtattaat
    17161 agtaatattt cttttagttt cttcggaatc aaagtctgtt gtggttgtac cagcatcaac
    17221 cttacccaag cggttaatag cacctgaagt atagatcatc gcttcagcga gagaagtctt
    17281 acccgcacca ccatgggata tgagggctac atttctaatc atttctgtgg tatagttttt
    17341 cacgccaatg cctcctttaa gctttgccag aaaataagga caattgtgca gaacacgttt
    17401 aaacttcgac cttggtttgg ttaaatcctg ctcagactgt taatatttct cttgacgatc
    17461 ttttttatgt ttaaagttgc taattttata gtaatcctgg taaaaaggca agaaaccccc
    17521 tccagactat ccccagcctc ttggcgacac acgcatacaa aagacgctcc cattttctga
    17581 tggaagcgtc tttttagtta cttattgctg ctgctgtaaa cgccattgca cgtaaccgat
    17641 taaaccaccc ttggccataa tattttgcat aaagggcggg aagggtgccg cttgatatgt
    17701 ttccttgcta gttagattat aaatagtacc gttctctaaa tctactcgta tttcgtctcc
    17761 tgccgcaatt ttctccgccg cttccggaca tgccaaaatt ggtaatccta tattaatcgc
    17821 attgcggtaa aaaatacggg caaaggaatg ggcaattacg cagctgatcc ccgccgcttt
    17881 aatcgcgata ggtgcatgtt cacgagaact gccgcagcca aagtttttta gcgcgacaat
    17941 aatactgcct ggcgttactt tttgcgcaaa agtgggatct gcatcttcca tcacatgctg
    18001 cgctaaagca ctggcctctg aggtgttcaa atagcgtgcg ggaataatgg catctgtatc
    18061 aacatcgttg ccaaacttcc aaaccatgcc ttgcatttac aacacctccc taggggcaga
    18121 aatcttaccg gtaatggcac tggccgctgc tacagcaggg ttggacagat acacttcact
    18181 gccaggatgg cccattctac ccacaaagtt gcggtttgtg gtagccagcg cccgttcacc
    18241 ttctgcgagg attcccatat gcccacccaa gcatggcccg caggttggcg tagatactac
    18301 agcttctgcc tctagaaaaa tatctactag cccttcctgg atggcctgac gcagaatatt
    18361 ctgggtgcca gggataataa tcacgcgcag atttttagcc actttgttac cccgcataat
    18421 ctccgccgcg atgcgcaagt cctccatacg tccattggta caggagccga tcaccacttg
    18481 atcaatccgt acctctccca cctcactaag gcctcgcgtg ttttccggca gatgagggaa
    18541 agcaacttgt ggttcaatat cttctccctg gaaagtaaat attttttcat acgcagcatc
    18601 ttgatcggcg taaaccggag taaagtcacg tttggcacgt gctgctgcat agtctagggt
    18661 tacttgatct ggggcaataa tcccacattt agcccctgct tcaatggcca tattactcat
    18721 cgacaagcgg ctgtccatag ataaagctga aatcatctca ccagtaaatt ccatggcccg
    18781 atagagagct ccgtcgacac caattttacc gattaagtag agaatcaaat ctttaccgct
    18841 gacccaaggc tgcagtcgac cattaaaaac aaattttaag gtggagggaa ctttaaacca
    18901 ggcttcaccc aaggccatag ctgcggccat atctgtactg cccaccccgg tagctaaagc
    18961 gcccaaggca ccataggtac aagtatggga atcggcaccg ataataattt ctcccggtaa
    19021 tgctaaaccc atttctggta ataaggcatg ttcaatgccc atgcgaccca cttcaaaata
    19081 ataagtaatg tcatattccc gcgcaaattc ccgcagcatt tttgcttgct gggcagattg
    19141 aatatctttg ttcggtgtaa aatgatcggg aactaaagca atacggtccc gatcaaagac
    19201 tttttctaca ccgagacgac gaaattcatt aatcgccacc ggtgaggtaa tatcattgcc
    19261 taaaaccata tcaactttgg cattgattaa ttctcctggc tgcacagtcg ttttatctgc
    19321 atgacgagcc aggatttttt ccgcgatggt catccccatt cctattctcc tcctttctta
    19381 aaagaccact atacttcttt ggctgcaatc caactcatca tgcggcgtaa ttttttgcct
    19441 actgtctcta gtagctgatc tttttccatt cgatccgtgg ccttaaagaa aggtctgtgc
    19501 gcttggtttt cttgaatcca ctctagagca aacttacctg tttgcacttc atgaagaatt
    19561 tgtttcattt cttgacgtgt ttcttcagta acgatgcgct taccacgggt gaaatcacca
    19621 tattgggctg tatcagagac agagtagcgc atataagata agccgccttc ataaatcaag
    19681 tccacaatta gtttcatttc atgtagacat tcaaaataag ctatttctgg ctgatagcca
    19741 gcttccacca aggtttcaaa accagcctta attaattcgc tgacaccgcc gcaaagcacc
    19801 gcctgttcac caaagagatc tgtttccgtc tcttctttga aggtagtttc aataacgcca
    19861 gcacgcgtcc cgccaatacc tttagcgtag gctaaaccca gttcagaagc tttgcctgta
    19921 tagtcctgat aaactgcgag caaggccggc acaccggcac cttctgtata agtacgacgc
    19981 actagatgcc ctggtccttt gggtgccacc atgaaaacat ctacattggt tggtggtaaa
    20041 atctgttgaa agtgaatatt gaagccatgc gaaaaaccta aagcatcccc gtcttgcaag
    20101 taaggtttaa tttcagcctc ataaacttgc cgctgtgttt catctggcag gaggatttga
    20161 atcacattag cagtctgggc cgcttccgcc accgtcatca cttttaagcc agcagcttct
    20221 gcagctgccc aactttgact atccttccgc agtcccacaa taacatctac accagattct
    20281 tgcaggtttt gggcctgggc atgcccttga ctgccataac ccatcacagc tactgtacgt
    20341 ccctttaaaa cattgagatt tgcatcatta tcataataca tttttgccat tttttagatc
    20401 ctccccattt ttcgttgttt tgctgccacg cagcaaagca atttttcctg tgcgtactac
    20461 ttctagaatg ccatacggtg ccagtaaaag ctgaattgcc tgcagttttt cttcattccc
    20521 tgttgcttcg ataattaatg aattaagggc cacatctaca actttggcgc ggaatgtttc
    20581 tactatttgc tgaatctccg cacgattaac tgcagttgct ttaactttaa tcaataccaa
    20641 ttcccgatgt actgaaggct ccagcgttaa attagagatt tttagtacat tgatcagttt
    20701 gtgcagttgc ttttctactt gctctaaagt ccgttcgtcg gcttcaactt caatagtcat
    20761 ccgcgaaata ccggggtcct gcgttctacc caccgccagg ctttcgatat taaagctgcg
    20821 gcgggcaaac aaacctgcta cccggaccaa aacgcctggc ttatcttcca ctagtactgc
    20881 taaaacatgc ttcatgcttc atgccccctg accatctcgc taataccgct attgggcggt
    20941 accatgggga agacattttc ttctgctcta acatgaaaat caattactac cgggccaggt
    21001 atggcaagag cctttttgat tgcggggatt acctcttgtg gtttttgcac acgcagccct
    21061 acagcaccga aagcttctgc cagtttcaca aaatcgggac tctgttgtaa attgacagag
    21121 gaataacgac gaccataaaa catttcctgc cattggcgga ccatacctaa atactgatta
    21181 ttaataatcg caattttcac agggaggtta taggccaccg ctgtagctaa ttcctgcaaa
    21241 ttcatttgca aactaccatc accggcaata cagaatactg tttgtccggg tgccccaatt
    21301 tgcaaaccta gagaggcggg caaaccataa cccatggtac ctaacccccc cgaggtcaca
    21361 aaggcacgcg gttttttaaa taaataatat tgcgctgccc acatttggtg ctgccctact
    21421 tctgtggcga ttaaagcctc accatttgtc acctgataga tagattgaat tacatattgt
    21481 ggtaagatct cgccctcttc tggctgcaga taagtaaggg gggcctgctt acgccactca
    21541 gcaatctgtg ccagccatgc ttcgtgcgtc tgtggtttaa ccttgcccag cagggtagta
    21601 agtacccgcg tcacatcacc gacaataggc acatgcacac taacattttt accaatctca
    21661 gctggatcga tgtcaatatg aataatcttg gccgtacgag caaagtggct taacttgcct
    21721 gtcacccgat catcaaagcg tgctccgacc gcaatgatca agtctgcctc atgcacagcc
    21781 atattcgcat agtaggtgcc gtgcataccc agcatgccta aagacaagcg atgggtaccg
    21841 ggaaagccgc cgatccccgt taaggtagta gtaacaggaa tctccgcttt ttctgctaag
    21901 gccagcaata attcatctgc ccctgaaatc acaacgccgc cgcccgcata gataatgggc
    21961 tttttagctt gggccatggc ttgagctgcc cggctaatct gaccaggatg tccatccagt
    22021 gtaggattat agcctgggat attgactgcc tgcggataag taaattcccc tgtcgccacc
    22081 tgcacatctg tcggcaagtc aatgaggata ggaccagggc gtcccgtttt ggctaaataa
    22141 aaagcttctt taatgattct cggcaattca ttaacgtccg tcactaaata attatgtttt
    22201 gtcactggca gtgtaatgcc aaaaatatct gcttcttgaa aagcatctgt gccgatcagt
    22261 gtggtagcta cctgaccggt aaaaacaacc agtggtaccg aatccatgta ggcattagcc
    22321 aagcccgtca cgagattagt agcgcccggg ccggaggtgg taaatactac ccctggttgg
    22381 ccagtaaccc gggcatagcc atcagcagca tgaatcgctc cctgttcatg acgcgcaaga
    22441 taatgttgaa tatctgcttc ataaaaagca tcgtataaac ttaaaacctt accaccggga
    22501 taaccaaaga ttacattaac ttgttcttct ttgagtgccg ccacaaccat ttctgcaccc
    22561 gtcattttca tgtagcatca acctccctga agacggcacc tgtattggca cttgttacct
    22621 gctgggcata gcgtgccaaa tagccagacg taattttagg ttgaggtaga gttaactttg
    22681 ctaaacgctg ctgcaattga tcatcactaa gcaaaagctc tagtttacgg gcaggaatat
    22741 caatagagat tagatctcct tcagcaatca cggcaatggg gccgcgctcc atggcttccg
    22801 gtgagacgtg accaatggag gccccacgag tagctccgga aaaacgcccg tctgtgatta
    22861 aagctacatc tttgtctaga cccatgccgg caacagagga agtgggggtt agcatctctc
    22921 tcatgcctgg gccgccttta ggtccttcat agcgaataac aatgatatct cctgcctgaa
    22981 tctttccgcc atagatagct tctacagcct cttcttcact atcaaaaacc cgcgctggtc
    23041 cggttttttg catcatttcc ggagcaactg caccctgttt gacgactgct ccatctggtg
    23101 ctaaattgcc tcgtaagata gcgataccgc cactgtcgct atagggattg gtaaaggggc
    23161 gaatgacatc ttcgtcaagt atggcagcct cagctagttg ttccccaagc gtacggccac
    23221 ttactgttaa gcatgctgta tgcagtaaat ctttttctgc caaccttttg aggaccgcct
    23281 gaatgccgcc ggcagcagct aaatcctcga tatggtattc tccagctgga gccaatttac
    23341 ataactgcgg gatgcgctca ctaatagttt gtatataatc caaatcaagt gtgatgcctg
    23401 cttcatgcgc aatcgccggc aggtgcaaga ctgtattcgt ggaacaacct aaagccatat
    23461 ccatcgtcaa agcgttcaca aaagcttctg ttgttaatat tttagctggt gtgagttcat
    23521 tgtgaatcgc cttcatgact tcaatgcccg cctttttggc caagcgcacc ctggcagcgg
    23581 ccacagccgg cacagtaccg ttgcccggta aacccatgcc taaagcttct gtaatgcaat
    23641 tcatcgaatt cgctgtaaac ataccggaac aggagccgca accaggacag gcactttctt
    23701 ctagttccag cagctcatca gcagccatcg taccagcttt aacccgaccc acagcttcaa
    23761 acagactatt taaatcagcc ggctgaccct gaaagcgacc cgctaacatc ggaccgccgc
    23821 tcacaaagac agctggcaaa ttgagccgag ctgctgccat taacatcccc ggcacaattt
    23881 tgtcacagtt aggaataaac actaaaccat ctaaaggatg agcctgcacc ataacctcta
    23941 cggtatcagc aatcaattcc ctgctcgcta aagagtagtg catgccctgg tgattcatgg
    24001 ctaagccatc acacacgccg atggtgaaaa actccatggg cgtgccccca gctaggcgta
    24061 caccctcctt gacagcagca gaaatctgat gcagatgttt atgaccaggc acaaaatcat
    24121 tagcagaatt aacaataccg attagcggtc gttccatttc ttctttgacc cagcccatag
    24181 catataacaa agaacgatgg ggtgcacgtt ccaccccttt tttcatttta tcactgcgca
    24241 tagctcttcc tcctattttt acgctaaatc ctccggatat acaggttctc cctctgctcc
    24301 agccacagca cgaaaacgtg caattaaatc attggtaatg gaacctggtt taccggcacc
    24361 gatctgccgt ccatccactt cgacgaccgg aattacttct gctgctgtac cggtgaggaa
    24421 agattcatca gccacgtata attcatgccg ggtaaaaggt ctttctagga cggtgtaacc
    24481 ggctgctgcg gctagttcca taatgacatc cctggtaatg ccaggaaggg caccaacata
    24541 agagggtggc gtgataactt tattgttttt tataatgaaa atgttatcgc cggtaccttc
    24601 agctatgtaa ccctgcccat ttaacatcac tgcttccatc acaccggcgc gattggcttc
    24661 gatcttgacg agaatattat ttaagtagtt aagtgatttg atttgcggat ctaaggcatc
    24721 aggcgcattg cgccgcgtcg ctacagtaat cacggaaaga ccttgctcat ataattcctc
    24781 aggataaaga ctgatggagc tagcaataat tacagttgtt gctttttcac atttacgtgg
    24841 atctaaacct agatcacctg taccacggga aaccaccgca cgaatatagg catcttgcaa
    24901 attattggcg cgaatagttt tcaggattgc ttctttcatt tctgcatagc tttcaggaat
    24961 agtgagcatg atcgatttgg ctgattcata aaagcgagct aaatgttcct ccagacgaaa
    25021 aaccttgccc tggtaggaac gaatcccctc gaagacacca tcaccataaa gaaagccgtg
    25081 atcgaagaca gagaccttgg cctcctcttc aggtacatac tgaccattca aataaataat
    25141 cctacccatt ccaaatccct cctctaaaat ttaatccctt ggtatatacg aaaagagcgc
    25201 tttccccagt aagcatgctc ttcagtatta gctcagacct gacatatctc taccaaacac
    25261 tgcagacagt tgctttactg tctctcaaga cactctaaag cgttaattgc cagcaggaca
    25321 attgccatga tataaaaaaa aacccttgcc ccttggtatg ctaaggggcg aaaggattgc
    25381 tttcgcggta ccacccttat tctccgctca accggactcg ggttcctcac gataacgggg
    25441 agggcccggc tcaacttact aaccacacag ggctttcagc cagcagctta gaggggacct
    25501 tcagctgcgc tgtttaccgg tttgcagcac ccaccggctc tctgtaaaac agacgcaaaa
    25561 tactcttcct cttcattgcg tttatagata tctgctaaaa aatttgaatc taattatatc
    25621 taacacttta catcttgtca agatagtaat agctagtttt aggaaaaact ttgattctat
    25681 tttatggtag cagatctgct aatttttgcg tgtcgcaacc taaaactact tcttcaccga
    25741 caacaactgt gggaacaata gtatgctgag acttatcgat catgcgctgg cgcgcctcag
    25801 ggttgtcctc cacattatat tctacatatt ccagattttt atcggaaaga aactctttca
    25861 cgcgtagaca gtaacttcag ccatgcgcag tatacaattc aattggctgc attgccgatc
    25921 accccttttc actgctagtc tgaggcagca agaggcactt tatacccaat aaaaaaccct
    25981 tacttgcccg ggggcaaata agggtttgtg aataagataa tatttagatg gtttgttctt
    26041 tggtttctgc tatttccaga cgcaatactc tggccactgc tttgtaaaca gcaaaagtaa
    26101 tcacactatt ggcacccgct ttaattaaat taaaaggaat aataattggt accagcatgc
    26161 ccatgaccac ctcgcgggga gcacccatga aggccaccgt cagcactaaa tttagtggaa
    26221 tcatagctaa agtcatggcg atagaaccta aaatcagact gacgacagcg ccgccccgtg
    26281 aagtaaactt gtggtaaata gttcctgcga ctaaaacaaa agctcctgtt gcaatgatat
    26341 gcataatcgc acctatccaa ccactgccgg cagaaaccgt tactgcctgg atcagggaaa
    26401 ctactactgt gagaattaga ccactgatgg ggccaaagag gaaagtaccg ataagaatag
    26461 gcacatcagc catatcatac tccaaaaagg gagcggccgg gaaaatggga aaacgtacca
    26521 tatacaccaa taaaattgat aaagccgcca acattgccat ggtcaccatt tgtctgacat
    26581 tacttttcac gtctcttttg cctcctaata ttattgatgc ctggaaaatc aacactgtac
    26641 aaatcacaaa gaggcaattt gcacagtaga agcaaaaatg cccccgaaag ttcgggggca
    26701 tgacgaatat ctacggtaga tgaataaacc gctcatattc gcttctccca tccggactat
    26761 accgtcggcg ccggattcta accggctcag cgctagcgct cgcgggctta acggcttttc
    26821 gccgtatacc gccggtgggg aattacaccc cgccccgaag catcatctat attatatagt
    26881 ctctaaaatt aatcgtcaat aacaattttc gacatgcttg attttacctt aacgtttagc
    26941 ccactgagct gcttgttcgc cggctatttt ggcacttact acggcttccg ttaaaaatag
    27001 ttccgggatt gcttgcctgc cgtgcagccc cgtcgtcaat tctccggcag cataaagacc
    27061 ttcaagggcc ccctgctgtc ccatcaccgc atagtcttcg ctgactgtta atccccccgg
    27121 tgtcaaagct actaacccga gtacggcaac ttgatacggt gcctttagtt gctttacttg
    27181 ttccgcaacg gctgccggct cgctgtttaa tactgccgcc agcaaattaa catcatcaat
    27241 agtttgcgtt ttgatttgct gggctacagc attgccagct cctgccacaa caaacactgt
    27301 accattagcc cttaacagta tcttggttaa attatcacca gtcagcactt ggcccgcagc
    27361 attaaaaagc agtgcttctt gccatattaa ctgctcaatt ctcgcaccat cctgtactat
    27421 aacgggctgc agccagactt gagacatatt ttgcgtgatc ccacccaggg cttgggctaa
    27481 ctgcaggcca actccctgat gaccgccctc taaacgcggt ctagtaccct taaggcctgc
    27541 ataatctaac agcagctcct gattagaagc aaaaccccca tccgctaata caactgctcg
    27601 ggccgaaata tccagctcct ggccttctgc agtacggacc actaaacctt tcacgcggtt
    27661 tccctgctgc ttcagtcgta agggtaacca atctttttcc gccctgacca gttttggcaa
    27721 tcgtgctgct gtttcctgac aaagctcaga aagctgcctg gccgtcagct tgtgaaaaac
    27781 ttggtttgtt ttcgcctcat taaccaaatc aagacccgtc agactttcca gccactgtaa
    27841 actagcagca gactgtaaac aaaagtttaa tatttgggca tagttacctg cttctttgcc
    27901 gcgtgtgtat atttctaaag ccatagtttc aggcaaatag gcaggctctg cttccgccgt
    27961 caaaccaggt gacctggcag caaaccaaaa agttggtaca taaggcggca ctatttgctg
    28021 ctctgtttgc tgcagattaa gatacagcac atctgtatct tgttgtgccg cagcaattgc
    28081 tgtcagctca ccagctaaac cactacccac cacaactaca tctacactat acggcagcgg
    28141 cggatagtct ggtatttcat ttttccagga aaaacgaact aacagcacaa gcaggaggct
    28201 aaataaaagt aaagcagtta ggatcgcagc acgcagctct ttcattttgg ggtgagacat
    28261 ctcacacccg cctaggcgtc ttgatcatcg gcaaccacac gcgccaggct gaccacccga
    28321 tcaccctcat ctacacgaat gagcgtaacg ccctgtgtat gtcggccttg ttgtgagatc
    28381 tcggtagcat cctgcctgat gacaatgccg ccagctgtaa taatcattac ttcatcgcca
    28441 ggacgtacaa ttctggcccc aatgacagct ccttttttat ccattacacg gatggtataa
    28501 atacctttgc cgccgcgtgt ctgggtacga tattcaaaga atggtgtgcg cttgccaaaa
    28561 ccgttttctg tcaccactag cagctcacta tcgcgctcta attgaggatg tactacttcc
    28621 agggctataa cctggtcatc ttggcctaaa cttatgcctc gtacgccata cgcattacgc
    28681 cccatggacc gtacatcatt ttcattaaag cgaatagaga gaccatcttg cgttactagc
    28741 gctatttggt gttcaccatc tgtcagcctt acatcaatta attcatcatc atcacgtaag
    28801 gaaatggcga tgataccgtt tttacgggca ttagcaaaat cctctagtgc tgttttcttc
    28861 accacacctt gtcgcgttgc cataaacata tattgtcctg catcaaagtc acgtaccggg
    28921 ataacagcag taattagttc tcctggttct acagccatta aattaataat cgccgtacct
    28981 ctggcctgac ggcctgcttc aggaagctca aagactttct tgcgatacat tttaccacgg
    29041 ttagtaaaac aacaaacata agcatgggta gaggcgacaa agacatgttc aacaaagtca
    29101 tcgcctttag tttgcatggc ggtcacgccg cggccgccgc gccgttgttt tttatacgta
    29161 gtagctggta tacgtttgat ataaccgtta tgtgttaagg tcacgaccat atcttcttcg
    29221 gcgattaaat cctcgattaa aaagtcatct tcactcacga caatttttgt ccggcgttca
    29281 tcgccatact tgtcacgaat ctgcaattgt tcatctttaa taatctgcag caataatttc
    29341 tcattagcca agatttcttg gtaataggct atctttttaa tcaactctaa atactcttct
    29401 tctagttttt ctctttccaa accggttaat ttttgtaagc gcatatccaa aatggctttg
    29461 gcttgtcttt cgcttaactg gaaattatcc attaaccctt ggcgggctaa atctactgta
    29521 tgtgaggcac gaattaattt aatcaccgca tcaaggttat cgatcgctat gcgcaaacct
    29581 tctaaaatgt gggcccgttc ttctgctttg cgtagttcat attgtgtgcg tcgggtaata
    29641 acatcttttt ggtggtctag ataacagctt aacatttggc gcagattgag aactttaggt
    29701 ctgccattaa ctaaagctaa cataataatg ccaaaggtct gctgaagttg ggtgaattta
    29761 aacaattgat ttaaaataac ttgggcatta acgtctcttc ttaattcaat gacgacacgt
    29821 aaaccattac gatcggattc atctcgtaaa tctgtgatac cctcaatttt tttattgcgt
    29881 accaggtcag ctattttttc aattgtttta gctttattta cctggtaggg cagttcagtg
    29941 accacgatgc gggatttgcc gttttccatg tgctctattt gtgtcagtga cctgatttta
    30001 atgatgccgc gcccggtacg ataggcagag cgtatacctt ccttgcccac aatgagaccg
    30061 ccggtaggaa aatcgggacc cttgatccga ctaaaaattt cttgttcttc gcattcagga
    30121 ttatctatta aatagacaag agcatcaatt acttcacgca aattgtgcgg tggaatatta
    30181 gtagccatac ccaccgcgat tcccgtcgaa ccattgacta agagattggg aaagcctgct
    30241 ggcagcacaa ctggttcttg caaagtatcg tcaaagttag gccggaaatc aacagtatct
    30301 ttttgaatat cagctaacat ttcaacagcg attttgccca tccttacttc tgtgtaacgc
    30361 atagctgctg gtgggtctcc atcaacagaa ccaaagttac cgtgcccgtc tactaatgga
    30421 taacgaatcg aaaaattttg tgctaaccgc accatagcat cataaacagc tgtatcgcca
    30481 tgggggtgat atttacccaa cacttcaccc acaatacgcg ctgatttttt atgcgcttta
    30541 tctggccaca ttgataaatc agccatcgaa taaagaatgc gccggtgtac tggttttaag
    30601 ccatcgcgca catctggtaa agcacgacta acaatgacac tcatggcata atcaataaag
    30661 gagctcttta cctcatcatg aatagcaatg ggtactattt taccatgcgt aaaattcatt
    30721 acgacccacc tcctttaaat atcaagattg cgcacatact gcgcatgttt ttcaataaaa
    30781 ttacgtcttg gctccacttt atcacccatc aggatcgtaa agatctcatc tgcccgcatc
    30841 gcatcttcca tcgtcacttg cagaatcgtt cttttttctg gatccatggt agttgaccat
    30901 aattgatcag gattcatttc accaagacct ttgtaacgct gcaaagtaat gccgtcacgg
    30961 ccaatttcct taagtaagcg ttctaaatct tgatcagagt acagataata ttctttttgt
    31021 tttttcttta acttatataa ggggggctga gctatataga tgtagcctgc ttgcagcaaa
    31081 ggctgcatat agcggaaaaa gaaggttagc agcaaagtac ggatatgtga accatccaca
    31141 tcggcgtcag tcataataat tattttatgg tagcgtgcgc ggctgacatc catatccgct
    31201 ccaatcccac tgccaagggc ggtaatcatg gtacgaattt cttcgttgcc taaaatacgg
    31261 tcaagacgcg ccttttctac gttgataatt tttccccgca gcggtaaaat cgcttgaaat
    31321 ctacggtctc tcccttgttt agctgaacca ccggcagaat caccttcaac gatatacagt
    31381 tcactgattg cgggatcacg actgacacag tctgctagtt taccaggcag tgaagtgatt
    31441 tctaaggcac ttttacggcg tgctaattcc cgcgctttac gagctgcttc tctagcccgt
    31501 gctgcactaa tggctttttc caccaagcgt ttggcgatgg caggattttc ttcaaaatag
    31561 gtgcctaatt cttcgtagag aaaagaatcg actaaacctc tcatctctgt attgcctaat
    31621 tttgttttcg tctgcccttc aaattgtggt accaacagct tgacacttac cactgccgtg
    31681 agtccttcac gaatatcttc accactaata ttattctcgt tttcttttaa gagattttgt
    31741 ttgcgcgcat aatcattaaa gatacgtgtg agagcagttt taaagcctgc ttcatgcgta
    31801 ccgccctcat gggtgcgaat attattggca aaagaaaaaa tcaattcatt atagccatca
    31861 tgatattgta aggccacttc aatttcactc tgatcctctt tggcgcgctc taaatagatg
    31921 acttttttct gtaaaagatc tttattctta ttgagatatt cgacaaaaga cttaatacca
    31981 ccatcatatt tatatacttc tttaacttct tcttcactgc gtctgtctac cagagtgatg
    32041 cgaatacctt tatttaagaa tgctaattca cgtaaacgct gcgtcagggt atcaaagtca
    32101 aaattaagag attcaaatat ttcaggatct ggcctaaagg aaattttggt accagttttt
    32161 tttgtcttgc cgataatttt taatggcgat acagtttttc cgcgttcaaa gcgcatctgg
    32221 tgtagcttac catccacttg tacttcgact tccagccact ccgaaagggc attcaccaca
    32281 gaaacgccta ctccatgtaa gccgccggat actttatagc cttcaccgcc aaatttaccg
    32341 ccggcatgca gcacagttaa cacaacttcc acagccggtt ttttcatttt cttttgctcg
    32401 ccaaccggta taccgcgacc atcatcggtt actgtaataa tattttccgg ctcaatggtg
    32461 accaaaatat tatcacaaaa accggctaac gcttcgtcaa tactattatc tacaacttca
    32521 tagactaaat ggtgaagccc ccgttcaccg gttgaaccga tatacattcc cggccttttt
    32581 ctaaccgctt ccagtccttc aagaacctga atttgatcag cgccataatg gatttcgttc
    32641 tttattttat cttgtttttc tgccacgttc ctacctcccg ttgcccacac taactaccgg
    32701 cctgcttcgc taactgccag gcatagttat tgatagccag tgccagataa agtcctgctg
    32761 actagccatc agttacacgc tggcagcaaa ctattacatt ttagccagta agttagtatt
    32821 tatttttcta ttgtttcgaa tatttttgcc tgaaagcgtt ttcttaaagt agtaggtgca
    32881 atcggtgaaa aaagcacact tgactcggtt acaataaagg tttttgtctc ttcactgtct
    32941 tgctttgtaa tttgttttgc tgattgtaaa aattctttat tgcattgttt gtcttttagg
    33001 cgaatatcaa aaataccgat gatatccttg gcccgcacaa tgcgggaacc accaatatgt
    33061 agaaacaaat aaaaacctcc ctctaaccgc aattctaagt ctactaacta ttttacccat
    33121 tttattttct tttacagctt aaacaaaatg tttcgcgcgc cgtctgcact aacggcaagc
    33181 cgcaatgctc acaggaaggc tcaccttgtt caatcaccca cttttggtgt tttttttgtg
    33241 ccacaaaaag cgccctcata ctttgtttta attctgctgg taagttggac ccgtcaaagg
    33301 agtcttcaat acttgctagc tcttgttgcc ctaaacttat tgatcgccaa ttgccctcct
    33361 caggatgact gttattagca gcagcctgag tctgcagctt tcctacctga aaacgaagat
    33421 catgcaaaac tctagttttc gtagcacgct gcagtttagc aataatttga gttttttgca
    33481 gcgacagatg ctgagcccaa actgaatcac agacacgtac tagtagtgtg ccattttcaa
    33541 aagccagagc ttcagttttc atggcaatcc attcaccaac aacttcgctc cacgcatcaa
    33601 taagcatttg ccctcttatt tttttggcgg cgggtaaatt tttaagtgtt tttttaagaa
    33661 tttgagctat ggggatcact tgtcctcacc tctcccccct gcactataaa caatttacca
    33721 tcgtgatgaa gtactcccag cctttcggca gccgttccgg tgataattgt ctgtactttg
    33781 ccctcaatgg cagctactaa aagctggcga cgcttactat caagttcgga gaaaacatca
    33841 tccaaaagta aaataggaaa ttctcccgtc tcgcttttga tgaattcaat ttctgctaat
    33901 tttaaggcca agatcaaggt gcgctgctgg ccctgggaag cataaatacg agcgttttta
    33961 tcattaatgg ccaaaagaat atcatcacga tgtgggccga tcagtgtctg ccccatgcgg
    34021 cactcatgtc cagcatgttt gtctaaagcg tgtgcaaact tttcttccca atctgtgttg
    34081 ggtgaggtta ttttttcacc aatactagac agataatcaa ccgtcagtgt ttcttctctg
    34141 tctgtcaggc tgcggtgtgc taatcgagct aataaaccaa ggcggtgtac ggcaaaactt
    34201 cttttcgcga ttactttact gccctgttta attaactgct cattccagct ggccaattca
    34261 gccgaatgta aaactttttc tcgttgtgtt tttaataatt gattgcgctg acgtaagatt
    34321 tgttgatagc ggcttaaatc ttgctcgtag agtggagata ttttgccaat tagttcatca
    34381 aaaaactttc gccgcagctg cggcgaacct tttactacag ctaaatcttc cggcgtaaat
    34441 aaaacaggaa taacccgccc tagatatgca gcgcgtgata actccacacc attgcaaaaa
    34501 tattttttct ttcttgtttg cagatcatag ctaacgcgca gcttttgcag ctgtaatctt
    34561 tgctgcacac tggcaacaac agtgcatgat tctgatcccc agcgtaccag ttcttcttca
    34621 ttgcgggtac ggaatgaacg cgctagtgca gaaaagttaa ctgcttccaa aaaattagtt
    34681 ttgccctgcg cattttctcc caccaaaatg tttaaatgcg catcaagctt tagttctaaa
    34741 tgagagtaat tgcgaaagtt gtgtaagaca agggtttcaa cacgcaaaat gttactcctt
    34801 tgctcaatac tagctaatct taactggtaa aattaagtag aggtaattat cgtataatga
    34861 gtctctcata ataaatggtt gctcgtgacc atttaattca attaggcact gatcgccttc
    34921 agtaattttt agcatctcta acataaaacg ggcattgaag gctatttcaa cttcttcacc
    34981 gttaaactcg atcggtacta cttcttgaat ttttccatat tttgaggagg cccgcataac
    35041 cattgtatct ttattgatca aaaaacgtaa gacatgatta ttgccttccg ctaaaagaga
    35101 ggctctttct acggcctggg taaaagctgc tgtctcgaca atagctttac ctgtaaagga
    35161 tttaggaatg acacgagtaa tattaggata gttttcatca atcaggcgtg aagagataca
    35221 gatattgttg caattcagga agagttgatt ttgcattact gctgcctcaa tcatcgcctc
    35281 atcttctgta aagactcgta aaacctcatt gaggtttttt ccaggtacaa gaaactctaa
    35341 atcacttttg ctttctacag cgcaagtggt agtggccaga cgaaaagtat cagaagcaga
    35401 taaagttagc tcgtgctgcc ttaaggagaa aaacacaccg ataaaagcag gttttccttc
    35461 atcatgagaa acacaaaata gagtttggcg cagggctcgg cgtaaatctg ctactttaat
    35521 ccgaaaactg cattgtgcaa gttctggttg ttgttcagga aaattgggat aatcatctgc
    35581 actatagcca taaacttgaa attcagcagg tgcactggag atctgggtag tgaaattttc
    35641 aggattaact tcgatgagca cagaatctcc aggtaagtgg cgaataattt ccaccgtttt
    35701 acctggcaga acaacttgtc ccctctcttt gtgtgccgca gcaaattgag tttgaatacc
    35761 taattccaga ttagtcgctg ttaaagtcaa cttttctccg tcgcattcaa aaagcacgcc
    35821 ttctaaagca ggattaacac ttctcgtggg cattgctttg gcgacaatct gcagatgact
    35881 cgccaaaaga tttttcggca gcaaaagacg caatttaaaa cacctcactt atttgggtct
    35941 agggtcaaaa aattaatttg ctttgcttaa aaataattta gcaattggca gctgtttaca
    36001 caagtttttg gtataaagaa ctattactag catatatata taacttagta gtatcagtag
    36061 tagggggtgt ggatagtgtg tataaacggc tctaagcttg agagcatcgt ctttagcggc
    36121 tggggataag cccgtggata aacagaacag cttatccacc tcctccacat atcaacaaaa
    36181 gcagccattc tatccagaag taatcaacaa aatataagca tgttatccac aagttatcaa
    36241 cagctgttaa ttttgtcagt taattgttcc acaatgcttt gtaattgtgg gtttttcttt
    36301 aagtctgaaa ttattttttt gtgggcatgc atcacagttg tatgatcacg cccaccaaat
    36361 tcttcgccta ttttaggtaa agaagaatcg gttagttcgc gcgctaaata catggctatt
    36421 tgtcgtggca tagcaacatt ttttgttctt tttttggcct tcatctcctc tatttttaag
    36481 gcaaaatgtt cagccgttac tttttgaatt ctttcaatgg tgattatttt tggtttttcg
    36541 tgtgtagcaa gaatggtctg cagcgtttct tgtgccaaag gaatatcaat tctgcgcttg
    36601 gttaaagatg aataggcaat aacccggatg aaggctcctt ctaattcacg aatattacta
    36661 acgatattat tagcaatata atgaataaca tcatcatcca tcgtgatgtt ttcggaaaaa
    36721 gcttttttgc gcaaaatagc aatacgagtt tctaaatcag gtggttgaat atctgtaatt
    36781 aagccccatt cgaaacgtga gcgtaaacga tcttccaagg tcggtatttc tttaggtggg
    36841 cggtcactgg aaataataat ttgtttatta ttttcgtgga gagcattaaa agtatggaaa
    36901 aattcttcct gtgttgtttc ttttcccgcg agaaactgaa tatcatcaat taataacaca
    36961 tcgatcgtgc gatatttatt acggaattct actgttttat tatcacgaat agaattaata
    37021 aattcattag taaatttctc tgaagaaagg tacatgacac gattggcagg tgtgttatct
    37081 gtaacatgat gaccgatggc atgcatcagg tgagttttac caagacccac accaccgtaa
    37141 ataaaaagcg ggttataagc acgagctggt gcctcagaga cagctaaaga agcagcatgt
    37201 gcgaaacggt tactattacc aataacaaat gtgttgaagg tatatttggg atttaaccag
    37261 gacgtagata aagtacttgt aggtttctcc ggtcttcttg ttggtgggga agctactact
    37321 tcttcttgat taaaatcttt accatcatcg tcattgatac ggggtgtgac aaaatgaaca
    37381 cgataatttt cgttgcccac tacctcttgt aaagcatggg aaattaaatc tgcataccgc
    37441 ccttgcatcc aatctttagt aaagtcattg ggtacactaa tcactagtga ctcactattg
    37501 atagaaacag gttttgtagt ttttaaccat gtttcgaaac taggcttact catttttgtt
    37561 tcgatttgat ccaaggtagc ctgccagata tccagcagtt caaattccat ttacatgatc
    37621 ctcctcaagc aaaacactcc gcgttaatta gccagccagt agaaaaacta ttcacaagtt
    37681 atccacatac tacacaaggc aaaaacataa ataataagca aatagtgaga gttatccaca
    37741 atttaaagct gttgtccaca ttatctgtgg ataacacaca aaatagccta attagtaagg
    37801 ctctttttat gggagagaat gtagttgtgc acagggtgtg gatagattgt tatccacacg
    37861 attatccaca agcaccaaaa aactactggt tcctcactta ttactataaa gtatgtttgg
    37921 acaaaaatct tttattgtca actttgcagt agtgagctct tatgtagctt ctgaaacagg
    37981 gcagtgcaat aagattgatg ttagcaaaca agctctcagg atatacattc ttatcttaac
    38041 aaaatctggc caacgccgca atagtgattg ttaataactg ccgcaagatc aggaaaagca
    38101 agggttttac ccattagctt ctcttgacat caaaagatga aaaagttata atctttttga
    38161 tatgggtttc tttattaatt tgataaactg ctaactgcaa caggaggtgt aatcaatgaa
    38221 gcgcacatac cagcccaaag ttagaaaaag aaaaaaaata catggttttc gtcgccgcat
    38281 gtcaacgcca gcgggccgtt tggtaataaa aagaagaaga gctaaaggaa gaaagagact
    38341 atcggcataa agcaggtaga taagatgaaa aaaaacggtc gtatccgtaa aaatagtgaa
    38401 taccaacatg ttttcactaa aggacgctca ctagcaatga gagcactcgt actttaccaa
    38461 ttgccaaatc aggaaaaaga aaatcgtaca ggatttgttg ttagtaaaaa agttggcaat
    38521 gcagttacga gaaatcgtgt taaaaggtta ttcagggagg cttaccgttt acatgctgct
    38581 gatttagtgc aaggaaaaga ccttgtattt attgccaggc cccaggcagc taaattagac
    38641 tatcaacagg ccgccaccgc aatgaagcaa attctcaaga ggggcggtct gttttcagtt
    38701 aataagtcag cacaagttta gaaaaatgaa agatacgggt gagggaatgc aacggctatt
    38761 gctcttgttc atacgtttct acagaaaatt tttgtcacca ttcaaaacag ctagttgtcg
    38821 ttttactcca acctgttcac aatatgcgaa agaagcaata gagcagtatg gtgctgctcg
    38881 agggttgtgg ctagcaataa aacgcttaag tcgctgtcac cctcttcacc caggcggtta
    38941 tgatcctgtg gatgaacacg aaaaggaggc caaagaatga ttgcggccat tgtagatgtg
    39001 ttaaactcta tactactggc aataaatgat tttacgcata attacggctt gtctattatt
    39061 gtattaacga ttcttgtgcg tttagttatg tggccattaa tgagtaagca gttatcttct
    39121 gccaaagcaa tgcaggagat acagccggaa ctaaaaaaga tacaggaaaa gtataaaaat
    39181 gataaagaaa aactaaatca ggccacgatg gaattatggc agaaacataa aattaaccca
    39241 gcagccggct gcctcccctt aattgtacag atgccaattc tatttgcgat gtttcggatg
    39301 ttacaatctg cgccgactat tgacccgcag caatattttc tgcttggtat cgatatgcgt
    39361 gttgctttgc agtcgacagt taacggagca gcagtttggc agcagtaccc agggtattgg
    39421 attttagtca ttttatcagg actcactact tatattcagc aaaaactaac catgactgat
    39481 ccgtcacaaa aaatgatgat gactattatg ccgctgatgc tcttgtattt cagccttcgc
    39541 ttccccgcag gtttagtgct ctattgggta gttaataact tgctctccct ggtgcagcat
    39601 ttgttgattc atagacaacc accgaaggga gcggtaaatg aacaatgaag gtagtagaag
    39661 caactgctaa gaccgtcgaa gaggcaactc aaaaagcgct cgctgaaatt gctttaccca
    39721 cggaaagagt aaaagtggaa gtggttaccg aaccaagtgc agggtttttg ggcctaatag
    39781 gttctaaaca agcgcgtgtt cgtgtgacgg aaacaatcag ccccgctcag tatatgtatg
    39841 attttttaga gcagattatt ggcaaaatag gattaactag tacgatcgaa gttacagaaa
    39901 ctgcacaagt gttaaaattg agtgtgaacg gcagtaagat gggtatacta attggcaaac
    39961 gcggacaaac actcaatgcc ctgcagtatc tattaagcgt tgtctatcat aaaaagtttc
    40021 ctggacagga tgggcgcctg ctccttgatg tagaagatta tcgtgaaaaa agagaagaaa
    40081 ctttgcgcac actagcacaa aacttagcaa aaaaagccct ccgttctcaa cgtgaggttg
    40141 tgctggaacc aatgagccct caggaaagac gtatcatcca cacagaacta caggaacatg
    40201 ctgctgtgtc tacctatagt gaaggtgatg aaccatatcg caaagtgatc atcgcaccta
    40261 aataaaaaag caaccgccgc ctgcgaggcg gcggtttttc ttcacctttc actcctgtgc
    40321 cagggtcgtc aagggagttg aagcagttaa ggatcagaaa ggggacaaag catgtttgaa
    40381 gataccattg cggctatttc cacgcctcca ggcgaaggtg gaattggtat tgtgcgtatt
    40441 agtggacccc aagcagagca aatcggcaga agcatcttta gctttgccaa gcagccggaa
    40501 aatctgttaa gtcatcatct ttattatggc aaggtagtta atccgctgaa agagcaggtt
    40561 attgatgaag cacttatttc ctttatgcgt gccccccata cttttacgcg tgaagatgtg
    40621 gtggaagtaa actgccatgg cggcattatg ccgctcagta aaactttagc ccttatttta
    40681 gcccagggag cgcgtttagc agagcccggt gagtttacac agcgggcctt tcttaatgga
    40741 cgtatcgatt tagcccaggc agaagctgtg attcaaatca tcagagccaa aacagaggcg
    40801 gccatgctgg ttagtgtgcg gcagttaaca ggtcacctct cacaagaagt taagcaaatt
    40861 cgccgccgct tattagcaat gctggctcat gtcgaagcat cgattgactt tcctgaacat
    40921 caggatgtcg aagatttagc ccgtgaggaa atagccaata gtacacaagc tgcttttgct
    40981 caactagagg cacttttagc cacggcagag caagggagaa ttctacgtga aggcatcaaa
    41041 actgctattg tgggtttgcc taatgtgggt aaaagttcac tcttaaatgc cctgttgcgg
    41101 gaacaacgag ccattgtgac tgagattcct gggacaaccc gggatgtgct ggaagaaagt
    41161 attaatctag gcggcattgc cctgaccatt gtcgataccg caggtattcg tgaaacacgt
    41221 gatgaaatag aaaaaattgg cgtgcaactt agccgctcgg cgatagataa agcggattta
    41281 gtacttttag tgctagagct gacagcaggt ttgaatgagg aaaataaatt aatcctgcag
    41341 ataattaagg ataaaccctg tattattatt gctaataaga cggatttagc agcagaaccg
    41401 caagcggcag cagcgcagct aaaagcacag ttaactcctc taccggttgt gccgatgtct
    41461 gttttaaagc aacagggact gcaggattta gaggatacca ttatccaata tgtatttaaa
    41521 ggagccgtac aaacaccgga ttcggtactt gtgacttcta ctaggcataa aaatgccctc
    41581 ttaaaagcaa aaaatgcgct ctatgactta caaaatgccc ttgaggctgg ttatgcggtg
    41641 gacctgttgg cgatagattt acatgctgcc ttggacgcct tgggagaaat aacgggagaa
    41701 agcgtaggac cagaattagt agatgaaata tttagcagct tttgtattgg taagtaaaag
    41761 ttagggtgtg atgcagtgac ttatgtaggt ggagaatatg atgtaatagt cgtgggtgct
    41821 ggccatgcgg gctgcgaagc agccttagcg tcagctcgtt taggctgtaa gactctatta
    41881 ttaaccataa atttagatgc cattgccttg atgccatgta accctgccat tggcggccca
    41941 gccaaaggac atttagtgcg ggaaatcgat gctttaggcg ggcaaatggc ccacaatatt
    42001 gaccagacca gcatacaaat gcgcctgtta aatacgggaa agggacctgc cgtccatgcg
    42061 ctgcgcgcac aagcagataa aaaaatgtat atgtggctaa tgcgtaaaac tctggagaac
    42121 accgccaatt tagatataca tcaagcgatg gtagaaagat ttcaggtgcg cagcggtcaa
    42181 gtgcaaggtg tgattaccgg caccggtgcc ctctaccgtg ccgctgccgt agtgctgacg
    42241 accggcacat atttgcgcgg tagagttatc attggcgatt atcattatcc aggaggaccc
    42301 aatggtcaat tagccagcac taaattatct acttatctta agcaaatcgg cctagagctg
    42361 cgtcgcttta aaaccggtac gcctgctcgc gtgcatcgtg attctatcga ctttagtaaa
    42421 acaatcatgc agcctggcga tgaacagcac tacgcctttt cctttacagg accgccggca
    42481 gcaactcccc agattccctg ttggctcacc catacgacag aaaaaactca tcaaattatt
    42541 cacgctaatt tacaccgctc ccccctctat gctggcgata ttaaaggtgt aggacctcgt
    42601 tactgccctt cgattgagga taaggtagta cgttttaaag agcggcccac gcatcaggtg
    42661 tttttagagc cagaaggtgc cgatactgcg gagtattatg tacaggggat gtcctctagt
    42721 ttgccggaag atgtgcaggt tgccatgctg cgtagtattc ccggcttaga aaaagtaaaa
    42781 gtattacgta ctggttatgc cattgaatat gattgtcttg atccactgca gttaaagagc
    42841 actttggaaa caaaaactat agcagggcta tacagtgcag gacaaataaa tggctcttct
    42901 ggttatgagg aagcggcagc tcaagggtta gtggcgggga tcaatgctgc tttagctgta
    42961 caaggaaaag caccattgct gattgaccgt gctcggggtt atactggtgt tcttattgac
    43021 gacttggtga caaagggaac tttggagccc taccggatga tgacttcccg ggctgaatat
    43081 cgtttactgc tgcgccagga caatgcggat gccagattaa caccgctagg ctacgagatt
    43141 ggcttaattt ctccagaacg ttatcagtcc tttacggcca aaagttctgc cgtagctgca
    43201 gaaaaagagc gtctgcaaaa aattacaatt accgcaagtg agcagactaa cgaactgctc
    43261 caggcctggg gtagtcgacc gttaacaacg gccgtcaagg ccagccaact attaaagaga
    43321 cccgaaatca gctatcaaaa ccttttacag ttactccctg aagctgcact actctctacg
    43381 gaagcagcac aacaagttga gattcaagta aagtacgaag gatatattac taaacaagaa
    43441 cagcagatcg aaaagtttaa aagaatggaa cataaagtgt tacctgaaaa catggattat
    43501 cttaatttat cagggctttc acatgaggcg gcagaaaagc tcaagcagat taagcctcat
    43561 tctcttggtc aggctagtcg tatttccggt gtttcgccgg cagacatcgc tgtcttacta
    43621 gtccatctac agcaacataa aaaacgtgga ggacagggac atgtggaatg agttaagtca
    43681 tttaagtaca gatttaggca ttagcctgcc cgagactgct ccctcactct ttcgacgtta
    43741 ttatgagctg ctcatctacc acaataaaca aatcaattta acacgtatta cggaagcaca
    43801 ggaagtgatg attaagcatt ttttagattc actgatgctt ttagtaatat ttcctcaatt
    43861 gagtgggcca cttttggatt taggcagcgg ggcaggcttg ccagggattc cccttaagct
    43921 tgctcgacct agcttagagc taaccctact ggatgccttg caaaagagga ttaactttct
    43981 acagcagctt agtgaaaatc tgcaactaac aggagtaaac tatttacatg gtcgtgcaga
    44041 agactacggc caacaaatag cctacagaga aaaatttccg cttgtggttt cacgtgctgt
    44101 cgccaaacta aatgtccttt tggagttatg tctgcctttt gtggcagtag gcggcttttt
    44161 tgttgcctac aaaggcccag aaggtgaaaa ggaactgcag ttggcagaac atgcgctgct
    44221 ggccttggac ggcgagccgg taaaggtgtg gacctatgat ttgccgcggc aattcggcaa
    44281 aagaagctta ctggtgatta gaaaaaattc ccgcacgtca gctagatacc ctcggaaagc
    44341 tggcttgccg gccaaaaggc ctttataagg acttcccata gcgagggaag tccttatatc
    44401 attctgcagc caattttagc tgctttttac ttacgctgct aaaatcctgg caaaattcca
    44461 aaatatattc tgcaagcaga gaaataatgg caggatattc ctttggtttg tcgaataatt
    44521 agcgtgataa gaagatagag atggagggag attgatgagc gaagattggg gaagtttgat
    44581 gaatattgaa gaaatggagt atgagcaggg tgagttacag aaaatagaca tcaataaaat
    44641 tcgacctaat ccatttcaac cgcggaagtt cttcgcagcg gtaaagttag cagaactcat
    44701 gcaatcaatt aaaacgtacg ggctcctgca acctgttata gtgcgtacca tctcaggcgg
    44761 ctatgaattg gtggcgggtg aacggcgttt acgggcttgt caacaactag gctggacaca
    44821 aattcctgct gtagtgaaag atatttcaga aagtgccatg gccacgatgg ccttaatcga
    44881 aaatctacaa agggaggatc tctctttcct cgaagaagca gctggttatg agcgtttatt
    44941 gcgagagttt aaattgacgc aggaagtttt agcacaaaga ttaggtaaaa gccaatcgac
    45001 aattgctaat aaattgcgtt tattgaaact accggatagc gttaaggcaa aattaaggaa
    45061 tcatgatttg acagaaagac acgcacgcgc tctactgaag ttacctgatg aggaagcaca
    45121 aaacaaaata ttacatgaag tgtgtaattt ggggtatact gtcaggcaaa ctgaaaaccg
    45181 tgtggctgcc tacttaagta agttagcccc cccatctaaa gaacgtaaaa aggtaatcat
    45241 tcgtgatgtg cgcatcttcc ttaacacaat ccgtcaagct gtctctttat tggaatcagg
    45301 tggcttggga cctcgtctaa aagaaaagga ttgtggtgat tatttagaag taactattta
    45361 cgtacccaag agtaagagaa ctagtaagct agcagagcaa tacaaaaaag agacagaatc
    45421 agctatttcc caggtaattg cccatgaggc tgctaacaat taacgacaac tatcaacaac
    45481 gggcagtagt aaattacgct ttcgtgcagg aaatggtaaa agctgacgcg aataatagtt
    45541 agtactgcag agagcgaggt gatcttatga ctcgtattat agcagtggca aatcaaaaag
    45601 gcggtgtcgg taaaacaacc acttcagtta atttgagtgc ttgtctggca gcggaaggaa
    45661 aaaaggttct cctgttagat atagatcccc agggaaatgc cactagcgga atcgggttgg
    45721 aaaaaagaga gcttaagttt tgtatctatg attccttgat taatgaggtt cctttacaaa
    45781 cggttatcca gagaaatgtg ctgccgaatc tggatgtcgc ccccgctacc attcaattgg
    45841 cgggggcaga aattgaatta gtaccaacga tgtctcgcga agtaaagctg cgacgagtgt
    45901 tgtcagagat aaaagagcta tacgaattta ttattatcga ttgccctcct tctcttggtt
    45961 tgctgaccat caatgccctc tcagccgcaa attcattgct ggtacctatt cagtgtgagt
    46021 attatgcttt agaggggtta ggccaactca cgaacaccgt aaaaatggtt aaaaagcatt
    46081 taaatactga actagaatat gagggtgtct tacttaccat gtttgacgca cgcactaatt
    46141 tatctgtaca ggttgctgat gaagtaaaaa aattctttgg cagccaagtg ttcgccgcag
    46201 ttataccccg caatgttcgt ctgagtgagg cccccagcca cgggatgccc atcatcgctt
    46261 atgacgagcg ctccaaagga gcagaaacgt acatacaatt ggcaaaggaa gtgatcgcca
    46321 atgggcaaaa aaaggctggg taagggttta caggctttaa tccccgaaat tgatgagaca
    46381 cctctttccg atgccattga aattggtgtg gcagagatag aaattaatcc taaccaaccc
    46441 cgcaaaagct ttgatgaaga gaaattatta gagttaacac gttccattga acaacacggc
    46501 atcttacagc cgttaatcgt ccgtcctacg ggatcaactt acgagctggt tgctggcgag
    46561 cggcggctca gagctgccaa aaaggctggt ttagaccaag ttcctgtcct gattagatct
    46621 ttatctgacc gggaaatgat ggagattgct ttaattgaaa atctgcaaag agaagattta
    46681 aatcccttgg aagaggcaga ggcttataaa agattaatgg aagaatttag ctacacacag
    46741 ggtcagctag cagaaagagt gggcaaaagc cgttcagcgg tagctaatac tttacgatta
    46801 atcgccttgc accacgatgt caggcgggaa atcgttaccg gtaatctgac agaaggacat
    46861 gccagggcaa ttttaggcct gccattagaa aaacagcctg aagctgcccg aaaaaccatt
    46921 gaattgggct tgtcagtacg agaaacagaa cgtctggcgg gacaagtaca aaagaaaaca
    46981 tcagtccgcc agcagaagac acgcaagaca aaagatggct atattgttga tatagaggaa
    47041 agactacggg aaaagtacgg tacagcggtc aatgttcgta tctcaaataa aggcggcggg
    47101 aaaatagaag tccattttta tgaccttgag gaattagaaa ggatttgcga gactctattt
    47161 taataattgc cagggtagga aaacacaaaa tttgtttcac gtgaaacaat gtcgattaat
    47221 tcttcgttgt ttcacgtgaa acattattat ttgtcagagc tagataacaa atctagctgt
    47281 cccatctgat aagctcaata gaacaatact ctgcagccgg gcaaggaaag agtaggtatt
    47341 ttttaaagaa agggaataaa aagatgttct tgattttgca gggaaccata gtaaatggca
    47401 tagcaattgt tgtaggcggt ttactcggag tctgtttagg ccataaaatg cccgagagaa
    47461 taaaagagct gattatgcag ggtatttcgt tggctgtttt agtaattggt atgcagatgg
    47521 ctttgcagat gcaaaacgct gtgctaatta ttttcagttt gattattggc ggtgtgttgg
    47581 gcgagctgat tggtattgat gagtggctgc aacgagtagg tagctggtta gagcaacgag
    47641 cagctcgtgc tagttcaggg ctggctcgtg cttttgtttt tgcgacgcta gtctatgtgg
    47701 ttggtgccat ggcagtaacg ggtgccttgg agagcggcct gttaggccag caccaaacct
    47761 tgtacgtaaa agcaatttta gatggtttta cagccattgc ttttgctgca accatggggc
    47821 ctggagtgtg tttggccgca attcccgtgg tgttgtatca gggcggtatc gctttagccg
    47881 ccggttcact gcaaccctac ctttctcctg ccgtcttgtt ggaattgaca ggagtaggcg
    47941 gtattttgat tattgcgatc gcctttaatc tgttggccct aaaagagatt aaggttgcta
    48001 atttcttgcc tgcatttttg gtggttttgc tgtggttaat tgtcttgctc ccactatacg
    48061 caaattttag tgtttaaata ctggaggagt agttggtatg gaattatatt tatatctttt
    48121 ggcggggggt tcgctgcttt tactggtttt ttgctctatg cttgctgtgc ggctgaagag
    48181 gctgacacaa aggtatcaag agtttatgtc tggtacagat agtgtgagtt tagaaggaat
    48241 gcttgtgaat cttcacgctg atttgcgtag tgtagcagct gaaattaagg aaaaccgaca
    48301 acagatgtcc cttctgcagc acaatttcgt ctccacagtg cgcggcatcg gtgttgttcg
    48361 ttttaatgcc tttcaaaata caggcggtga tttaagtttt gccgtggcct tgctcaatgg
    48421 gaagcaggat ggtgtgatct ttagcagtat ttatggtcgc gaggaatcac gtttctatgc
    48481 caaaccggtt actgctgcac aatctactta taagctgagt gccgaagagc aggaagcagt
    48541 tcggcaggca actgcgatgc tcgtgacgca ggcagaaaaa ttgtaatgga gaaaaaatag
    48601 atgcataatg aaaagagata tttctttatc ataaatccca cagcaaaaaa tggccaggcg
    48661 gtagctgttt ggcagcagct gcagctcttt cttgacttac agcgtgtatc cttccaattt
    48721 gccatgagta aagatattgc tgatgtcgaa aaactagcaa aagcggccgc cgcagcaggt
    48781 gccattgttg tcggggtggg tggagacggc actctctcgc gcattgcggg ggctttagcc
    48841 ggtacaaaaa cgactttcgg cctgattcca gcgggtacgg gaaatgactt tgctcgtacc
    48901 tatcagattc ctgatcgtcc ggaagcagcg atgcagatta ttttaaatgg ttacaccatc
    48961 gctttagatc tgggcatggt taatggccgt tacttttata atgccgtagg ctcaggtttg
    49021 gacgcggagg tagtggcaga tgcaaatctt acttttaaaa gaattggtgg ttcttttggt
    49081 tatttattag cgttgctgcg ccaattaatc ctctatcgac cgcaaaagat aaaaattact
    49141 gtgaatgacg aagtgcatgt agttgaagca tggttagtga cggtggctaa tgcttgttac
    49201 tttggcagtg gcatgaaaat tgctccttgt gctaatcccc aagatggttt gttggatgtg
    49261 attgtcgtac aaggtattca tcgtctcaat tttttacgtc tcttccctcg tgtttaccgt
    49321 ggtcaacata ttaagcatcc tgcagtgcga gtttggcagg ggactaaaat cgccgtagag
    49381 tcggaaaagc ctttagctgt gcaggttgat ggtgacttat gcggccaaac accgctgctg
    49441 gtcacgatgc aagcagcgat ggtgcagctc ctggtacctg atcagcataa aacctcctgc
    49501 agctgagaaa actaactgca gaaggaggcg atggtaaaat ggagcgcacg attgcggtgg
    49561 aagagggttt aacgccagtt ctcaaatatc tccaggaaaa aggctatcat gtcgtaagtc
    49621 tgcagtctgg gcaaaacgca gatgcagcag tgattagtgg cggcgacaaa gatgtgatgg
    49681 ggatgcagac gattgtgcaa gacggcccgg tgattgacgc tcgtggtatg catccagagg
    49741 atgtattacg agcaatagaa ggaaaatggc gccattaatg ttaaaaaaag tttaactagc
    49801 aagcagcagt aagccgtccg tgtttagcgg gcggcttcgt ttgtagaaac agtaactatc
    49861 tccggcaatt caggcaatag caggctgata ccttgagcaa tactttcggc gaggttaaag
    49921 acaagattga gtctagtatt ttgtaaaaca taatattcta agcatccggc tacattaaca
    49981 atcccattga tatagatgtt gccgacagct gggagctcct tactaacccc tgctcccggg
    50041 cgcagggatc cacgcccaat atcaatggcg ccaacactct catttttccc caagcaagca
    50101 tcaatggcaa taatgtaagg atcagcaaag cgctgagaga tcgtaatgat ggtttctgct
    50161 aaatttacag catgcaccgg tgctgctaaa gtgccgtaaa cagcagcagt aggtgctgtt
    50221 tgcattctgg taagctgtgt gcccgttaaa gggcctagag catcaccggt agagcgatca
    50281 gtgccaatac aaagtactac taaatgctgt tggtcagggc aaaagtcttc cttgattaag
    50341 ctctgcaagg cgagtttgat ttcttgcgcg ggaaataaat ctgtatgcca aactcgataa
    50401 acactttgtt tattttgctt gctatgcggc ggataggtag acatagctcc cctccgtttc
    50461 gaatacgtat atgatagtat atggaaataa accgtagtta tgagcgttta gctgataaac
    50521 taactgttta tgccatttcc cgggtaatat ctacaaaaat tgcaggagga aaaataatgt
    50581 cttgggttga tttggcggcg ctgagtatta tgctatgggg ggcggcgcaa ggctatctga
    50641 atgggcctct gaccgcgatt atacgacttt tgggatcaat tttagctgtg ctgatggctt
    50701 tttttctggt aaatccctta gccgtttatg tgcaaaatga gtggcagcta gaggctattt
    50761 ttgtcgattg gtttcagcgg acaggcaaag cagccatttt agtcacgcaa gctggggagc
    50821 aatatttaaa tttgcccacg gctgctgccc cactactgcg taagctggta ccggagttag
    50881 ctgcttcccc ggtcagcgga gcgcaggagg ctttagtgcc gctcttagca gctctggctg
    50941 tacgtatctt aattttaagt gctgccctga tctttatcct ggctgtaatc agggtgttta
    51001 cacaaattaa agctgcggca gccagtagta accagccgga atggcagcgt cttcttggtt
    51061 tagtcgttgg cagtgcagat ggtttattct ttgccctctt gacctgcgta gcactcgatg
    51121 ccttctccct tttagtctac cctacgctca tccaaggtga tttaaccagc tcttatcttt
    51181 ggcgagcagc aaattatctc ataaactaac gagtgttaat gctatgtaca gcagaattgg
    51241 aggcacacaa aagcatttac tccttgcttt aagcaacttg ttgcaggatt tttctaagaa
    51301 gggacagaat ataactagat aatccatgaa tactgaggaa aaggtggtta atcatgcgca
    51361 tagtcttact aggcccgccc ggtgctggga aagggacaca aggtgaacgt ttggtaaaaa
    51421 aatacaacat gccccatatt tcgacaggag atatcttccg tgctgcgatc aaggaaggaa
    51481 ctgaactagg cttacaagcc aaagagtata tggatcaggg acaactagtg cctgacgagt
    51541 tagtagtgga tattgttaaa gcaagactta ctcaaccaga caccaataac ggttatctgc
    51601 ttgacggttt tccgcgtaca gttgcccagg cggaagcttt agatgctaat ctgcaagagt
    51661 taagcacgcc gcttacaggt gtgcttaatg ttgatgtggc agaagaagag ctcattgagc
    51721 gcttaacagg caggcgtgtt tgtcgggctt gtggctccac atatcatatt aagtttaatc
    51781 cttctactgt acgaagtgtt tgcgataagt gcggcggtga gttataccag cgtgcagacg
    51841 atactgtaga aactgtcaag cagcgtctaa cagtttaccg tcaacaaaca gcacctttaa
    51901 ttaattatta tcgtgagcag ggaattttat ttacggtaga cggatcaaga gaaatagaaa
    51961 tagtctttca ggaaatcacg ctccttcttg atcaaaaaca ataatttagt ttcgcaaagc
    52021 agggtgatgg tatgctgcag tatttatcac aactaaacat ttcccgaacc gctatcgagg
    52081 attattttat agacatttcg ctaacttggg gttcacgcat ctttaaaggc atcctcatta
    52141 ttttacttgc ccgccttgct ttgcgttttg gtcaaaatgt cattgcgcgt ctcctgacgc
    52201 cagggcagca taagccttct atcttaccag aaggtaaggc taaaacactg cttggcttgt
    52261 tattttccct gtggcactac atcgtttatt cctttgctat cctgctttta ttaagtaacc
    52321 tagaaataaa tatcggtccc attttggccg gagcaggcat tgtcggttta gccgtaggtt
    52381 ttggcgcaca aaacttggta cgggatgtca tttcgggttt ttttcttatt ttagaagatc
    52441 aatttaccat tggtgaatat gtgactgttt tagagtatag cggtaccgtg gaagaaattg
    52501 gtctgcgtat tactaaaatt aggggctggg atggagaact atatataatc cctaacggtt
    52561 taatcgcctc agtaactaac tggaatcgtg gacatatgcg ggctttagtt gaagtcggcg
    52621 tagcctatga agaggatctt gaccgagtac ttacattgtt agagcaagta gctgtcgaat
    52681 atgctgccac tgatgccgat gtagtggcag ggccaagcgt ggtaggtgtg atggcccttg
    52741 ctgattcatc tgttgtgatt cgtgtcgtgg ctacaaccat ttctatgagc caatggaaag
    52801 tggaaagaaa cttacggctc gcgattaaaa ggaaatttga cgaagagggt atagaaatac
    52861 cctatccgcg tcgtgtcttg gagtaccgca gtaaacttaa gggggaggat tcccgtggct
    52921 gattttacag ttggcgaaaa ggtgaggatg aaaaaagtgc atccttgtgg cggcgatatc
    52981 tggacaatcg ttcgggtcgg catggatttt cgcatcaagt gtatcaactg cggccgcagt
    53041 attatgcttc cccgcagtaa attcgaaaaa aatgtacgcg aacgtcttta attgtctaca
    53101 agacttgtca gcgcaaattg agtatgatac tatataggat gtacttccct gctcccgcca
    53161 tagcgggggc caaagtccat agggaggagg tgagtaggcc atgacaaagt acgaaaccat
    53221 gtttatttta agacccgaac aagacgatga tacctataat acaatggtag agaaatttac
    53281 ggggattatt gaagccgaag gcggcgaaat tactaatatc aaccgtatgg gacgtcgcaa
    53341 actggcctat gaggtaaaga aacagtttcg tgaaggttat tatgtcctca ttaactacac
    53401 agcagaaccg agtgtaacta atgagctgga acgaaacttt aaaatttctg atgatgttat
    53461 tcgttacctc atagttcgag aggaagatta agcggggagg gtgaaagaag aatgctaaac
    53521 cgtattattt taatcggtcg tttaacccgc gatccggaat tacgttatac gtcaacaaac
    53581 ggcgtagctg ttgctagttt cactttagct gttgatcgcc gttttacaca ggggcagcag
    53641 cgcgaagctg actttatacc tattgtcact tggcgtaacc aagcagaaaa ttgtgccaag
    53701 tatttgggta aaggtagttt agtggctgtc gaaggacgct tacagatacg ctcttatgaa
    53761 aaagatgggc agcgtcgtac tgcggcagag gtggtagcag acagtgtgca gttcctggac
    53821 aaaagggaaa agcgttcttc cgatgatgcg ccttcttttg acgatgctat gattggtgat
    53881 gacgatgtac ccttttaaat ttaaggataa ctgctccgta aggagtttag cttgatccgc
    53941 agaggaggtt tgaagttatg cgaagggatc gcggccgcag aggcagacgt aaagtctgca
    54001 acttctgtgt tgataaagtc gaacatatcg actataaagt aacaaataaa ttagcacgtt
    54061 ttcttacaga aagaggcaaa attttgccac gtcgtatttc cggtaactgt gcaaaacatc
    54121 aaagacagtt aaccaatgcc atcaaaaggg ctcgcaatat cgccctgctt ccctatacgg
    54181 ctgagtaaca ctttagtaat cataagcgtc ctttacgggc gctttattct tcggaaaaga
    54241 attatctaac ttgctggaag cgactctttt ttaaaggaaa taaaaaggca aactgttgat
    54301 ctaaacagga aaaaagcttg ctctccccca ttgaagacgc aagctttttt ttagcatcta
    54361 gcaacgaacc acagagacag atattagtaa accctacatt ttttagcggc aggagattgc
    54421 tcaggttgtg cagaacttgt acagcactta ctgttgctgt tggtacgagg aggtatttaa
    54481 gatgaaagtt attttagcgc aagatgttaa agcgctgggt aaaaaaggcg atgttaagga
    54541 agtggcagaa ggatacgccc gcaactatct atttccccgt ggtttagccg ttgaggctac
    54601 cggcggtcaa ctaaaagaac tacaacaaca acaacaaaga accgctgaaa agcaggctaa
    54661 agttttagca gaagctgaac gcaaggccgc taaaatctcc ggcatgcaaa tagaaattag
    54721 tatgcgcgtg ggtgaaaacg gacgactttt tggttctgtt accagtggcg atttagccgt
    54781 tgcattgcag aaaaagggtg taaaagtgga caaaagaaaa attgagctgt cagaaccgat
    54841 caaaaatctg ggcagctatc cattacggat taagttacac tccgaagtag acgcaagctt
    54901 aacagtcaaa gtagtagcgg cagcccctaa ataatcatca ggagtaaata atgaaaaaaa
    54961 aattgacaac tagatcaata gaaacaaacg acttttcggc tgagccagaa caacaaaaaa
    55021 aagaaattaa tgcactttat gcgctgcttt cccaactgct tggtgctgat agattaattc
    55081 ttaaagccgg taaactggaa tcattgtcac tgttacgctc gcgacaacca gcagagcgct
    55141 tgctggggct gcagagagtc attttagaga accccacact ctctgaggtg cctactgcgg
    55201 cacagattcc cctgattttg cagcagcttt atgacgagtt agcagacaac atggcgcgtc
    55261 gtactgtgga ggagaatcta gaaagaaaag ttgcggaaaa aatgcaggaa aaacaggatg
    55321 aatatctgca tgaaatgaaa cggcagctgc ttaaggacga aggcggcccg gaaaatccgc
    55381 agacattaaa aaaatatgcc caactagaaa aaatgaaaca caccaagctt aaccgttcgg
    55441 ccttagagat tttacgtccc catacactga cagagattgt cggacaagaa gcggcggtta
    55501 cttctttaat ggctaaatta gcctctcctt atccccaaca tatcattatt tacggcccgc
    55561 ccggtgtggg taaaacaaca gctgctcgtt tagccttaga agcagctaaa cagttagcag
    55621 atagtgcttt ttcaccgcag gcccccttta tcgaagttga cggctctact ttacgctggg
    55681 atccacgcga aacaaccaat ccgctgctgg gttctgtaca cgatcccatt tatcaaggtg
    55741 ctaaacgcga tctggttgat ggtggtgtgc cggagcccaa acctggcttg gtttcagatg
    55801 cacatggagg cattttgttt attgacgaaa ttggcgagct ggatctccag ctgcaaagta
    55861 agctgctgaa ggtgttggaa gataaaaagg tttcctttga ctccgcctat tacgatccta
    55921 ccgatgcgca ggtaccgcag tatatccgag cactctttga ggaaggagct ccggcggact
    55981 ttgtcttgat tggcgcgacc acttgtgcac cagaagaaat taaccctgct ctacgttccc
    56041 gttgcacacc agtctttttt gccccgttga caccacaaga tattgaaagt attgtggttg
    56101 aggccgcagc aaaattgtct gtaaccttag ccaacgatgt ggcggcttta attgccagcc
    56161 ataccaccga gggtagacaa gccgttaatc tgcttgctga cgctttcggt ctcacacgtt
    56221 accgccagca gcaacagact gaagctgtca ttagtaaaga actggtgtta gaaacattgc
    56281 gtgctggccg ctatagtcct caaaccagta aaaaatatac tggcggcgaa catcttggta
    56341 gaacatatgg cctaggcgtc agcggctttc taggtagtgt gatagaaata gaggcggtgg
    56401 cttttcccgc taatattgct ggtaaaggcg gtatacggtt taatgaaaca gcaggcagta
    56461 tggctcgtga ttctgtgttt aatgccgctg ctgtcattcg ccagcttacc ggcgaggatc
    56521 tacagaacta tgacttgcac gtcaatgtga tcggcggcgg tcgtattgat ggtccttctg
    56581 cgggtctggc cattgtttta gctttactaa gtgccattaa ggggtggccg ctgtcacagg
    56641 aagtagcggt cacaggagaa atttctctgc agggttatgt acgccctgtc ggcggtatac
    56701 ctgaaaaact atatggagca cgcttggcag gtattaacac tgttgtggta ccgtatgaaa
    56761 ataaggatga tatccccagt ctgcccggca tgcaggtgat tcctgtaaaa aaaatcgagg
    56821 acgcactacc attgtttttt aaaggtcaag aagaagttat ttcacatcat tagtttttcc
    56881 gaaagaggtg taccaattga gcgggatgat ggaaagagta cccccgcaaa acttagaagc
    56941 tgaacaatcg gttttagggt caatgctcat tgatcgtgat gccattgtgg cggcagctga
    57001 gctgctaaaa ccaactgatt tttatcgcgg cggccatcaa aatatctttg cggccatgtt
    57061 agcgctttct gaaaaaaatg aaccggtcga tttaatcact ttggccgagg ccttaagcca
    57121 gcaaaaccaa ctggaggcga ttggtggcgt tgcttatctc accactttag ccaatgtcat
    57181 gcccacagcc gctaatattg gttactatgc caaaatcgta caagaaaagg ctgtgctgcg
    57241 gacgatgatt catgctgcta cgggcattgt ctcacgctgc tttgaggcta acgattcctt
    57301 ggaagaaatt gttgatgaag ccgaaaaaac gattttcgaa atttcgcagc gctccacgcc
    57361 tcaaggtttt gccaatatga aagatattct taagggtgct tttgaccgca ttgacaagct
    57421 ttggggcaat aagggcgggg taactggtgt gcctaccggt ttccacgacc tggacaatat
    57481 tacttgtgga ctccagaatg ctgatttagt gattatagca gcgcggccta gtatgggtaa
    57541 aactacgctt gctttgaata tggctcaaca tattgcggtg aacgagaaac tgccagtagc
    57601 aattttttct ttggaaatgt ccaaggaaca gttggtgcag cgtttgctct gtgcacaagc
    57661 caatattgat gcgcaacgct tgcgccgcgg ttatctctcg gatgaagatt atcccaaatt
    57721 aacccgcgct gccgggccgc tgtcggaagc accattgttt attgatgata cagcagccct
    57781 ttcagtgatg gaaatgcggg cgaaagcacg ccgtctcaag gcagaacatg gcctttcggc
    57841 tatatttgta gattatttgc agctcatgcg tggcagcggt cgtgtagaaa atagacagca
    57901 ggaaatttcc ggcatttcac ggtcactaaa ggccttagca aaagagttag atgtccctgt
    57961 cgtggctcta tcacagcttt cacgggctgt cgaacaacgc gagaaaaaac gtcctatcct
    58021 cagtgactta cttgaatcag gcggcattga ggcgaatgcc gatttagtag cttttattta
    58081 tcgtgaaggt tactacgatc aaaatatgga aaaccgccat gatacagaaa tcattattgc
    58141 caagcagcgg aatggtcctg taggttcagt ccatctttac tttaaggaga accataataa
    58201 gtttcttaat attacaacag agcaagtaga acaggttagt taaaagaagc atcacgctgt
    58261 taaaaaatgg ccattgacaa gtatcatgga atatagtaat atgttgattc gtaaggcgtt
    58321 tttgggccat tagctcagtt ggcagagcac ctgactttta atcagggtgt cccgcgttcg
    58381 agtcgcggat ggctcaccat tttggcccct tggtcaagcg gttaagacac cgccctttca
    58441 cggcggtaac gcgggttcga gtcccgcagg ggtcaccatg aaaaagttaa gagaccttaa
    58501 cag
//
LOCUS       DASRMS010000090         8977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_424292, whole genome shotgun sequence.
ACCESSION   DASRMS010000090 DASRMS010000000
VERSION     DASRMS010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 8977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8977
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424292"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="VFC74_03945"
     CDS             <1..341
                     /locus_tag="VFC74_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HZK24518.1"
                     /translation="PTKETIKRATIRDMKALGTHKPEYNRLIDTYADLVAQYCEINQR
                     FKNSDYAFQVPTADGGSKKAPIVATLENLRKDILAYSDRLCLNPKSLETVTAETGKKS
                     VLAEALKKLS"
     gene            371..2059
                     /locus_tag="VFC74_03950"
     CDS             371..2059
                     /locus_tag="VFC74_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HZK24519.1"
                     /translation="MEYANSIVEGRKVACKETIQMCQRFLDDLKNPAYDFKPKDAEFV
                     IGIIKTTFVHQKGEDMDGHPLRGRPFLFEPWQKFVVYNLLGFFHKGTILRRYKEAFIM
                     LPRKSGKTPFMSALAWGIGLLERKSGAEIVIVGALLKQALQSFNFLLYNIRNMGDDGW
                     FRILDNNQEHSISGDLDDGYMRIETIAGNSDRMDSLNTLVQILDELHLYKNASQYNTI
                     KESGKAYRNSLCIGITTAGDDMTSFCYQRKKYCQKILDGTVKDEQYFVLIAKADVDEA
                     GNVDYTNPIEHEKANPNYNVSVSGRELMNDAEQAQNDPQQRKSFLAKSLNVYTSAMKA
                     YFNLDEFRASDRKYKWTMAELARLPVEWYGGADLAKLHDLCGTCLYGSYGDVDIAITH
                     AFFPVVAAHVKADEDNIPLFGWQDDGWLTMTNTPVTDYQAVVRWFLEMKKKGFYIKQI
                     GLDKKFGREFHLEGRKAGLRVVDAPQLYFFKSEGFRRIEAKVKSSKFYYLHSDAYEYC
                     VSNVRAIEQVDDAVRYEKVMPNQRIDLFDASVFAAMEMLKNLQKAGTARKWLNG"
     gene            2173..2904
                     /locus_tag="VFC74_03955"
     CDS             2173..2904
                     /locus_tag="VFC74_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZK24520.1"
                     /translation="MQLTKICTKCGKVFPATKDHFHAAKLGKYGLTAICKGCSAKYKK
                     DYRRKNKERISEQNRQYYMENREHCLTVRGIWRKRNPEYFKEWYERNKDYSAQYDKYY
                     RKQNGHRIRDRQSNWYHRNKDRIIRMRAQYKKENRFKVRTWENKRRTMKAALPCDLTP
                     EDWVEIKASFGYKCAYCGRKKSLTQDHFVPLSKGGAFTKDNIIPACLSCNCSKHDKDF
                     NEWYPNHKHYSRAREDKILDHLGHK"
     gene            2969..4180
                     /locus_tag="VFC74_03960"
     CDS             2969..4180
                     /locus_tag="VFC74_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009624273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HZK24521.1"
                     /translation="MNLLKRIKNIGRKRADPSISQDYAAHWLLTQGGLLDDLNVPGYT
                     RLSNNPEVRMAAHKIAELISSMTIHLMQNTADGDIRVVNELSRKIDINPYSLMTRKAW
                     VYNIVYTMLLDGNGNSVVFPKIKNGLIDELVPLQPWQISFGPTDAGYEITYGHKKLAH
                     NEVLHFAINPDPNEPYRGRGYRVVLRDIANNLKQATATKKGFMSGKYMPSLIVKVDAM
                     TAELASEEGREGVYQKYLESSEAGRPWIIPAELLEIDQVKPLTLQDLAINDAVEIDKR
                     TVAGVFGVPAFFLGVGKYNKDEYNNFINSTILPLAKGVEQELTRKLLYSPDLYFKFNP
                     RSLYAYDIKELADVSGNMYVRGIMTGNEVRDWLGLSPLEGLSELVILENYIPAGMIGD
                     QKKLNPKGGED"
     gene            4180..4764
                     /locus_tag="VFC74_03965"
     CDS             4180..4764
                     /locus_tag="VFC74_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA];
                     GO_process: GO:0019082 - viral protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HZK24522.1"
                     /translation="MRDKKQTRSLQTELKTRAETDGENLYIEGYFAVFNRQTEFWPGA
                     FEEIAPGAFDNTLGNDVRALINHDSTLVLGRNKSGTLELKTDNHGLWGRVKINPNDTD
                     ALNTYERVKRGDVTGCSFGFNIVSEETEWRDDGSVKWTITGVDLHEVSICTFPQYEDT
                     GVQARKAEVAQHQQRQLEVRKKKLKERLSKCLGN"
     gene            4749..6041
                     /locus_tag="VFC74_03970"
     CDS             4749..6041
                     /locus_tag="VFC74_03970"
                     /inference="COORDINATES: protein motif:HMM:TIGR01554.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HZK24523.1"
                     /translation="MLRQLVLAKKLKALRKEIGGLDTQQGALTKKRDELAAAIEEAGT
                     DEELQLVEEEVTKVEGEQVELTEKKSKLEEEIAKIETELQELNSKEPTNNDRKRGVGG
                     MRDAEMRKTIIDFVRTKGQSLKHYREEPESNLPEDVGFKVIVDPEDSGSLIFPEEIMA
                     PVKEKREVVDLERYVRTIPVNRGSGKIPIIKKSGGRMVTVEELKQNPALEHPQFAQVS
                     YDIDTYRGYIPISEEAVDDADYNVIGLIAEEIADQELNTKNDAIAGILKDAPAPSYTG
                     TGELKTADPVEGLDGLKEVLNKKLKRIYNAKLFVTSSLYHELDTVKDKEGRYLLQDSI
                     TAASGKSFAGREVVVLDDDILGDDDTNLNAFIGDAREYCGLFDRKKVSLRWMDNNIYG
                     QLLAGVVRFDTGVLDENAGFYVTYEPEKNEAGEEGGNE"
     gene            6053..6205
                     /locus_tag="VFC74_03975"
     CDS             6053..6205
                     /locus_tag="VFC74_03975"
                     /inference="COORDINATES: protein motif:HMM:NF019139.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho termination factor N-terminal
                     domain-containing protein"
                     /protein_id="HZK24524.1"
                     /translation="MPASAMPVDLEAMTKTELMDFANEHGIEGLNARMLKADIIVAIK
                     GAMGWM"
     gene            6196..6474
                     /locus_tag="VFC74_03980"
     CDS             6196..6474
                     /locus_tag="VFC74_03980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24525.1"
                     /translation="MDVNTVLGLVKARIGLTTEVRDTYLVAIIEGVTSELGDEKGLVL
                     DGTNPYHLMFVVDFATWRYQNRDEPGALPRHLQFRLHNLIIHHGGDSQ"
     gene            6471..6803
                     /locus_tag="VFC74_03985"
     CDS             6471..6803
                     /locus_tag="VFC74_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0044423 - virion component [Evidence
                     IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HZK24526.1"
                     /translation="MTYDHELVLIAWGVEEDEIGQQIPVKTRTQILCKVKSIGRSEFY
                     DAAMTGLKPEIVFVIHEAEYGEEREVEFEEQKYKVLRTYKGGMTSHGSKLAFDEMELT
                     CERMADDG"
     gene            6796..7194
                     /locus_tag="VFC74_03990"
     CDS             6796..7194
                     /locus_tag="VFC74_03990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HZK24527.1"
                     /translation="MAKTVSIDQLENELTGVIKEYTENVSRGIDEEVKRTADLVLKET
                     KAKAPRRIPEYYKHFKKTEEGKVLAAYGRREYYVWNKKHYRRVHLLEFGHLKRSGKGR
                     VDAFPHLRPAYDNNAQDLDQRIERIIREGG"
     gene            7203..7517
                     /locus_tag="VFC74_03995"
     CDS             7203..7517
                     /locus_tag="VFC74_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24528.1"
                     /translation="MTQAELVQELKSLGMPVAYSHFIDQVSLPYLVYQFVYSNDMMAD
                     DVNALEVSNFQIELYTRTKDLAKEKLVQDKLKSLYLPYHKVETFIESENLRQVIYEIQ
                     LM"
     gene            7541..8143
                     /locus_tag="VFC74_04000"
     CDS             7541..8143
                     /locus_tag="VFC74_04000"
                     /inference="COORDINATES: protein motif:HMM:TIGR01603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0019068 - virion assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major tail protein"
                     /protein_id="HZK24529.1"
                     /translation="MGKGVAVGLRNVHFALLTDDDKTGCGYEDPVKIADAITVNVNPN
                     ASVAVLFADDGPSETATSIGEISLEFTAAVISSEGQAVLLGSIQGEDGVLRERATDSP
                     PYVAFGYERTMSNGKRRFVWLAKGKFIPGSTDSQTKEGTVTFQPDKLTGTFVKRAYDD
                     VWRMRANEDDEDFSGANEWFTSDMLESEGDESTNEGDGGD"
     gene            8218..8550
                     /locus_tag="VFC74_04005"
     CDS             8218..8550
                     /locus_tag="VFC74_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24530.1"
                     /translation="MQVPKLKIGDKEYRAGTLKMKAWRNIVKVQKDIGKIDQVKAMQD
                     EDLMFKMAKTLEVMFNNPDVTAELILEEMDVMDFVPAVQKVSEWVTMQVVGKLGEFPK
                     NSPMTGRS"
     gene            8601..8735
                     /locus_tag="VFC74_04010"
     CDS             8601..8735
                     /locus_tag="VFC74_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24531.1"
                     /translation="MWGWTQQQVDETELGHYFDLMIVAAKERAVKYKEDLAQIDALGM
                     "
BASE COUNT         2789 a         1729 c         2379 g         2080 t
ORIGIN      
        1 caccaactaa agaaacaatc aagcgtgcaa cgatccggga tatgaaagcc ctgggtacac
       61 acaagcccga atataaccgg ctcattgata catacgcaga tttggttgct caatattgcg
      121 aaataaacca gcgctttaag aacagcgatt acgcgtttca agtgcccacc gcagacggcg
      181 gcagcaaaaa agcacccatt gtggccacgc ttgagaattt acgaaaagat attttggcat
      241 actccgaccg gctatgtctg aacccaaaga gcctggaaac cgtaacggct gaaaccggta
      301 aaaaatcggt actggccgag gcacttaaaa aattaagttg acagtaaaaa acaagaacta
      361 cagtgtggta atggagtatg ccaacagcat tgtcgagggt agaaaagtag catgtaaaga
      421 aacaatccaa atgtgtcagc ggtttcttga cgatctcaaa aacccggcct acgattttaa
      481 gcccaaagac gctgaatttg taatcgggat cattaaaaca acctttgtcc atcaaaaagg
      541 cgaggacatg gacggccacc cactacgggg caggccgttt ttgtttgagc cctggcagaa
      601 atttgttgtt tacaacctgt taggattttt ccacaagggg acaattttaa ggcgatacaa
      661 agaagcattt ataatgctgc cccggaaatc tggtaaaacg ccatttatgt cggccctggc
      721 ctggggcatt ggactgctgg aaagaaagtc cggggctgaa attgttatcg tcggggcatt
      781 gctcaaacaa gccttgcaaa gttttaattt tttgttgtat aacatccgca atatgggcga
      841 tgatggctgg tttcgcatcc tggataataa ccaggaacat tctatttccg gcgatctgga
      901 tgatggttac atgcggattg aaaccattgc tggcaactcc gaccgcatgg acagcttaaa
      961 caccctggta caaatactgg acgaactaca cctctacaaa aacgcaagcc aatacaacac
     1021 gatcaaagaa tctggtaaag cataccggaa cagtttgtgc atcggcatca ccaccgccgg
     1081 ggatgatatg acgtcttttt gctaccagcg caaaaaatac tgtcaaaaaa tccttgatgg
     1141 taccgttaag gatgagcaat attttgtgct aatcgccaaa gccgatgtgg atgaggcagg
     1201 gaacgtggat tacactaacc cgattgagca tgaaaaggct aacccgaatt acaacgtgtc
     1261 agtgtcgggc cgggaactca tgaacgatgc agagcaagcc caaaacgatc cacaacaacg
     1321 caagtcattc cttgcaaaat cacttaatgt ttacacgtcg gcaatgaagg cctattttaa
     1381 cctggatgaa tttcgggcca gtgaccgcaa atacaagtgg accatggcgg aactggccag
     1441 gctacccgtg gagtggtacg gcggggcaga cctggctaaa cttcacgact tgtgcggcac
     1501 ctgcctgtac gggtcctatg gtgatgttga tatagccatt acccacgcct tttttccggt
     1561 ggttgcggcg cacgttaagg ccgacgagga taacatcccg ctgtttggct ggcaggatga
     1621 cgggtggcta acaatgacta acaccccggt gactgattat caggcagtag tgcggtggtt
     1681 tttggagatg aagaagaagg gtttttatat taagcaaatc ggtctagata agaagtttgg
     1741 gcgcgagttt caccttgagg gaagaaaagc gggtttaagg gtggttgatg ccccgcagtt
     1801 atactttttt aaatccgagg gcttccgacg tatcgaagct aaagttaaat caagcaaatt
     1861 ttactaccta catagtgacg catacgaata ctgtgtatct aatgttaggg ctattgagca
     1921 ggtcgatgac gctgttaggt acgaaaaagt aatgcctaac cagcgtattg atctctttga
     1981 tgcaagtgta tttgcagcaa tggaaatgtt aaaaaatttg caaaaggcgg gaaccgcaag
     2041 aaagtggctt aatggctagg tttatgataa aatgtggttg tgggatagcc cgacggggcg
     2101 acaagcatgg tttcccgacc atgtttccca cattagtata attcgggacg cactacggga
     2161 ggtgtatttt ttatgcaatt aacaaagatt tgtacaaagt gtggaaaggt attccctgcc
     2221 acgaaagacc acttccatgc ggcgaagttg gggaagtatg gcctaacagc catttgcaag
     2281 gggtgctcgg caaagtataa aaaggactat cggcgcaaaa acaaggagcg aatatctgaa
     2341 caaaataggc aatattatat ggaaaaccgg gagcattgtt tgaccgttag ggggatatgg
     2401 agaaagcgga acccggaata tttcaaagag tggtacgagc ggaataagga ttattcggct
     2461 caatacgata aatattatcg gaaacaaaat gggcacagga taagggaccg gcaaagtaac
     2521 tggtatcata gaaacaaaga tagaataatc aggatgcggg cacaatataa aaaggaaaat
     2581 cggtttaagg taaggacatg ggaaaacaaa aggcgaacaa tgaaggcagc gctaccatgt
     2641 gacttaacgc cagaagattg ggtggagata aaagcgagct ttggctataa gtgtgcctac
     2701 tgcgggagaa aaaagagcct tacgcaggat cactttgtcc ccctctcgaa ggggggagca
     2761 ttcaccaaag ataacatcat ccccgcctgc ttaagttgca attgcagcaa gcacgataag
     2821 gattttaatg agtggtatcc taaccataaa cactactcaa gagcacggga agataaaatc
     2881 ctagatcatt taggacataa gtagaaaaga aaagcactta ctaaaaagta ggtgcttttt
     2941 tatgtgcaac taaaaaggag gtggataggt gaatttactt aaacggatta aaaacattgg
     3001 acgaaagagg gctgatccca gtatatccca ggattatgcg gcacactggc tacttaccca
     3061 gggtgggctg cttgatgatc ttaatgtgcc gggctacacc agactaagca ataacccgga
     3121 agtgaggatg gcagcgcaca agattgcgga gctgattagc tccatgacca tccacctgat
     3181 gcaaaatacc gccgacggtg acatccgggt tgtaaacgag ctatcccgca aaatagacat
     3241 taacccgtat agcttaatga cccgcaaagc atgggtttat aacattgttt atactatgct
     3301 gctggacggt aacggtaaca gcgtggtatt tccaaaaatt aaaaacggat tgattgacga
     3361 gttagtcccg ctgcaaccct ggcaaataag ctttggacct actgacgccg ggtatgaaat
     3421 aacttacggc cataaaaaac tggcccacaa tgaggtacta cacttcgcca taaaccccga
     3481 tccgaacgaa ccataccgtg gccgagggta ccgggtagtg ctcagggata ttgccaacaa
     3541 cctaaagcaa gccaccgcaa ctaaaaaggg atttatgagc ggtaaataca tgcctagcct
     3601 aatagttaag gtggatgcca tgaccgctga actggccagc gaggaaggcc gggaaggggt
     3661 gtatcagaaa tatcttgaat cctccgaagc cgggcggcca tggatcatcc cggctgaatt
     3721 attggagatt gaccaggtga aaccgttgac tctgcaagac ttggctatta acgacgctgt
     3781 ggagatcgac aagcgcacgg tggcgggtgt ctttggtgta ccagcgtttt ttttaggcgt
     3841 cggcaagtac aacaaagatg agtataacaa ttttattaac tctactatcc tgcccctggc
     3901 taaaggcgtg gagcaggaat taacccggaa actattatac agtccggact tgtatttcaa
     3961 gtttaacccc cgcagtttat acgcctacga cataaaagaa ctagccgatg taagcggaaa
     4021 catgtatgtc cggggcatca tgacgggcaa cgaggtccgg gactggctgg gattatcacc
     4081 gctggaaggt ttgtcggagt tggttatttt ggagaactac ataccagcgg gcatgatagg
     4141 cgaccagaaa aaacttaacc caaaaggggg tgaggattaa tgcgagataa aaaacaaaca
     4201 cgcagtttac aaactgaatt aaaaactaga gccgaaaccg atggcgaaaa cctgtacatc
     4261 gaggggtatt ttgcagtgtt taataggcag acagaatttt ggcccggagc ctttgaagaa
     4321 attgcacccg gtgcatttga caacaccctg ggcaatgatg tccgagcctt gataaatcac
     4381 gacagcactt tggtgttggg gcgcaataaa tcaggcacct tggaactcaa aacagacaac
     4441 catggcttat gggggcgagt aaaaataaac cccaacgaca ccgatgcgct caacacctac
     4501 gagcgggtta aacgggggga cgttaccgga tgttcctttg gctttaacat tgtgtccgaa
     4561 gaaaccgagt ggcgggacga cgggtcggta aagtggacta tcactggcgt cgacttacac
     4621 gaggttagca tttgcacctt ccctcaatat gaggataccg gggtacaggc cagaaaagcg
     4681 gaagttgccc agcaccagca aaggcaatta gaggtaagaa aaaagaaatt gaaagagagg
     4741 ttgagtaaat gcttaggcaa ttagttttag caaaaaaact gaaggccctg cgcaaagaaa
     4801 ttggggggtt ggacacccag cagggagcac taacaaagaa gcgcgatgag cttgcagcgg
     4861 ccattgaaga agctgggaca gatgaagagc ttcagctggt ggaagaagaa gtaaccaagg
     4921 tcgagggtga gcaggttgag ctgaccgaaa agaaaagtaa actggaagaa gaaattgcaa
     4981 agattgaaac cgagctccag gagcttaact ccaaggagcc aacaaataac gacagaaaaa
     5041 ggggtgtggg tggtatgagg gatgcggaaa tgcgcaaaac aattattgac tttgtaagga
     5101 ctaaagggca aagcctgaag cattacaggg aggagcctga aagtaacctg cctgaagatg
     5161 tgggtttcaa agttattgta gacccggaag attcgggatc gcttattttc ccggaagaaa
     5221 ttatggcacc ggtgaaagaa aaacgggaag ttgtagacct ggagaggtat gtgcggacta
     5281 tcccggtaaa ccgtgggtcc ggtaaaatcc cgatcattaa aaagtccggc ggtcgcatgg
     5341 tgaccgtcga ggaattaaaa caaaacccgg cacttgagca cccgcaattt gcccaggtta
     5401 gctatgacat cgacacgtac cgcgggtata ttccgatctc cgaagaagct gtggacgatg
     5461 cggattacaa cgttatcggt ctaattgccg aagaaatcgc tgaccaggaa ctcaacacca
     5521 aaaacgacgc tattgccggt atactcaagg atgctccggc accgtcatac acggggaccg
     5581 gcgaactgaa gaccgccgac cccgtggaag gtctggacgg gctcaaagaa gtgctaaaca
     5641 aaaagctaaa gcgcatttat aacgccaagc tgtttgtgac atccagcctg tatcacgaac
     5701 tggacaccgt gaaggacaaa gaagggcgct acctgctgca ggacagtatc actgctgcgt
     5761 ccggcaaatc cttcgctggc cgtgaggtgg tcgtgctgga tgatgacatc ctgggtgacg
     5821 atgacaccaa cctgaacgcc tttatcggtg atgcccgtga gtattgcgga ttgtttgacc
     5881 gcaagaaagt atcgttgcgg tggatggaca acaatatcta cggccaactg ttggctggcg
     5941 tagtgcgttt tgataccggg gtgctagatg agaatgctgg gttttatgta acctacgaac
     6001 ccgaaaagaa cgaggctggg gaagaagggg gaaacgagta gaggcgttca gcatgcccgc
     6061 ctcggcgatg cctgttgacc tagaggcgat gaccaagact gaactgatgg actttgccaa
     6121 tgagcacggc attgaagggc tgaacgcacg aatgttgaag gcggatataa ttgtcgccat
     6181 taagggggcg atgggatgga tgtaaacact gttttggggc tagtaaaggc tcgtatagga
     6241 ctaaccactg aggtaaggga cacatacctt gtcgctatca tagagggcgt tacaagcgaa
     6301 ttaggagacg agaaggggtt ggtgcttgat ggtaccaacc cttatcattt gatgtttgtg
     6361 gtggacttcg ctacttggag atatcagaac agggatgagc caggggccct gccccggcac
     6421 ttgcaattca ggcttcacaa cctcattatc caccatggcg gtgatagcca atgacctatg
     6481 accatgagtt ggtgctgatt gcctggggcg tggaagagga cgaaatcggc cagcaaatac
     6541 cggttaaaac aagaacgcaa atactttgca aagtgaaatc tattggtcga tctgaatttt
     6601 acgacgcagc tatgacagga ctaaagccgg aaatagtctt tgtaattcat gaggcagagt
     6661 atggcgaaga gcgggaagtg gagtttgagg aacagaaata caaggtgctc aggacttaca
     6721 aaggcggcat gacctcccat ggctccaaac tggcctttga cgagatggag ctaacctgcg
     6781 agaggatggc tgacgatggc taagacggtt tctatagatc aactggagaa tgagcttact
     6841 ggggtaataa aggaatatac agaaaatgtt agcagaggta ttgatgaaga agtcaaaaga
     6901 actgctgact tagttttgaa ggagacaaaa gccaaggctc cacggagaat tccagagtat
     6961 tacaagcact ttaaaaagac ggaagaaggt aaggttttag cagcatatgg taggcgtgag
     7021 tattatgtct ggaataaaaa acattaccgg cgtgtgcatt tgttggaatt tggtcattta
     7081 aagagaagtg gtaagggccg ggtagatgcc tttccccatt tgcgacctgc ttatgacaac
     7141 aatgcacagg atttagacca gcgaatagag cgtattatta gggagggggg ctaaaggtag
     7201 gtatgacgca agcagagcta gttcaagaac tgaagtcgct aggtatgccg gtagcctaca
     7261 gccattttat tgaccaggtt agccttccgt atcttgttta tcagtttgtt tacagtaatg
     7321 atatgatggc tgatgacgta aacgctttgg aggtaagtaa ttttcagata gagctttata
     7381 ccaggacaaa agatttagct aaagaaaagt tggtgcagga taagctaaaa agtttatatt
     7441 tgccttacca caaggtagag acatttattg aatcggaaaa tttaagacaa gtaatctatg
     7501 aaatacagtt aatgtaatgt aatttaagga ggaaaaacat atgggtaaag gtgtagcagt
     7561 tggattgagg aatgtgcatt ttgctttgtt gactgatgac gataaaacag ggtgtggata
     7621 cgaagatccg gtaaagatag cggatgccat taccgtgaat gtcaacccta acgcttcagt
     7681 agcggttttg tttgcggatg atgggccatc tgaaacagct acaagcatcg gggagatttc
     7741 actagagttt actgcagcgg ttatttcatc cgaaggtcag gccgtgttgt tgggtagtat
     7801 tcaaggtgag gacggtgttt tacgagagag ggccacagat tcccctcctt acgtagcgtt
     7861 tgggtatgaa agaactatgt caaacgggaa aaggaggttt gtgtggcttg ctaaaggcaa
     7921 gtttattcct ggatctactg attcacagac aaaagaaggg actgttacct tccagccaga
     7981 taagttgacc ggtacttttg ttaaacgtgc ttatgatgat gtatggcgta tgagagcaaa
     8041 cgaagatgat gaggatttta gtggtgctaa cgaatggttt acttctgata tgttggagtc
     8101 agaaggagac gagagcacta atgagggtga tgggggcgat taatagcggt catttaggcc
     8161 gcttttcttt tgcttgataa atacaaaaca aaactaaaac aaataggggg ttaaaatatg
     8221 caagtaccaa aattaaaaat tggggataaa gagtatagag ccgggacact gaaaatgaaa
     8281 gcctggcgaa acatagtgaa agtacaaaaa gatataggca aaatagatca ggttaaagct
     8341 atgcaggatg aagatttgat gtttaaaatg gctaaaacat tggaagtaat gtttaacaac
     8401 cctgatgtaa ctgcggaatt aatcctagaa gaaatggacg taatggattt tgttcctgct
     8461 gttcaaaaag taagtgagtg ggtaacaatg caggtagtcg ggaagttagg ggaattccca
     8521 aaaaactccc ctatgacggg ccggagttag aattaaatcc gtatcaaacc gtcatataca
     8581 gttatttgca agctgcacgt gtgtggggat ggacacaaca acaagtggac gaaacagagt
     8641 taggacatta ttttgattta atgattgtag cagcaaagga gagagcagta aagtataaag
     8701 aggatctagc acaaattgat gccctgggaa tgtagcttgt gacatggtat aataccccta
     8761 atatgccagt atttaaaggc cgtagggcct ttttcttttg taattgtaga ggtaggtgag
     8821 gtttatttgt caaaaaacac agttaaactt gagtgtgata tatgtaagaa ggagtttata
     8881 ctgtttgggg taggcataga ggttgttagg gatgatgtct ggaaacacgg ttttttgtgt
     8941 cctcactgta aaacagaata tattgcatat tatacag
//
LOCUS       DASRMS010000091         3572 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_759983, whole genome shotgun sequence.
ACCESSION   DASRMS010000091 DASRMS010000000
VERSION     DASRMS010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3572)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3572)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3572
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_759983"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VFC74_04015"
     CDS             complement(<1..533)
                     /locus_tag="VFC74_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24532.1"
                     /translation="MIDEKQPQQQITPNETNRAAAQAESDAKAKAQAVEAQKKEKAVF
                     ERIIKKRNEKKRLSEVEKTILELAATYDAAHQDEKDIYHALLNTLVREDEAKAAERRQ
                     ARERKKLIKDKLDKLHDTRARHKANNALHVANNLYKLGILNNDASYKIPAEIFYSFIK
                     ANLHLLNQPVEQAEAPKT"
     gene            859..2229
                     /locus_tag="VFC74_04020"
     CDS             859..2229
                     /locus_tag="VFC74_04020"
                     /inference="COORDINATES: protein motif:HMM:NF015354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HZK24533.1"
                     /translation="MSVANLRVSIGSRQYKSTSGKMRERSALAKILYILRRARHKTAQ
                     DLMHESSGNMPYWAAEDPTKFWAAADKHERKNGTLFIEHLLVLPRDLTLEQHIELVER
                     WAETEASHRAYTYAIHYSQSADGGINPHCHFVLNESLQDGVKRGESKFFKRYNAKKPE
                     RGGCKKGGTGARTPAENKQVLIQQRQLFEDLCNEALERAQKDTRVSLQSYKNRGLDIV
                     PEPKVGPRGWRNEKIRNEILAMREAKKELAKELTTIQKTQVVALEQYRQIKEKQAALA
                     RQEKAQERLALQQREREREQEAVKAKNDFWQQEQAEYEQFVAGVEQQEAEIIDFIALQ
                     AEATYQASKADFMQLFVNELTQVAPIYFELAQQDLIGFMHYAAIQADMELDDAVVRVF
                     VEKEKPVLMAQIAERMRAYRQGLVKPQALMYLMQAKEIKESVSFEQLIAELEQQAVYK
                     PPKPDG"
     gene            2411..3331
                     /locus_tag="VFC74_04025"
     CDS             2411..3331
                     /locus_tag="VFC74_04025"
                     /inference="COORDINATES: protein motif:HMM:NF015076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HZK24534.1"
                     /translation="MMLEQKKLFIENLPHKPYCTDNLDYGLQIRSVKIALTKRLIQHN
                     KPFGVSWLVFDLDSPHFWSLLESETLPAPNLVSFNLENHHCHIYYSLKTPVCRSNVAR
                     RRPLRYLAAIEYALREKWTADAGYTGFISKNPIHPHWETRQFRSEPWELGELADWLTL
                     PKTLPRRGQSVGLGRNCTLFDMLRYWAYDNVLEFRINSNRNSWSDTVLSTAESFNSFP
                     EPLPTAEVRATAKSVSNWVWENYTKRWSDEEFSQVQAARGRMSGAKRRHASKEKRAKA
                     YELRAEGMTQQAISKELGVNQSTVGRWLKR"
     gene            3386..>3572
                     /locus_tag="VFC74_04030"
     CDS             3386..>3572
                     /locus_tag="VFC74_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24535.1"
                     /translation="MFDNGLFVGALLVEINNKKSLPPPVAYYFLPIIKHGLKNKLPVP
                     LWSVAKSGAETSEARSVC"
BASE COUNT          997 a          732 c          921 g          922 t
ORIGIN      
        1 gtcttaggtg cttccgcttg ctcaacgggt tggttgagta agtgtaaatt tgctttgata
       61 aaagaataaa aaatctcagc gggtattttg tagcttgcat cgttatttaa gatacctagc
      121 ttatagaggt tgttagccac atgaagggca ttgtttgctt tgtgtcgtgc gcgcgtgtca
      181 tgcagcttgt ctaatttatc tttgattagt ttttttcgtt ctcgtgcttg gcgtctttcg
      241 gctgctttgg cttcgtcttc cctgactagc gtattcagta gcgcatgata aatatctttt
      301 tcatcttgat gggctgcatc atacgtcgca gctaattcta gaattgtttt ttcaacttca
      361 gaaaggcgtt tcttttcatt gcgtttttta atgatgcgct caaacacggc cttttctttt
      421 ttttgcgctt ctaccgcttg cgcttttgcc ttggcatcac tttctgcttg agctgctgct
      481 ctattcgttt cattaggcgt tatttgttgt tggggttgtt tttcatcgat catattattt
      541 cactcatcaa aatagggtgg gtgtttttgc actgtacgat cacgaaatag aaacaacaag
      601 ccaacttttg cagaaaagtc attgttcttt ttacattgca gctttttcct cattgctttt
      661 gcttaagata ggcgggccat ttatattgac ttatgaaaca gccctgcgca gcagccctcg
      721 gagagcgcgg ttaggggttt caaaaaaagc tccgctttgt cttacgactg cttgcgcatt
      781 tagaaacccc aacctcgcgc aaagggctgc gccccttgac ccccttaacc gcgcgcgctg
      841 tgcctattgg agagagtgat gagtgttgca aatctgcgag tcagcatcgg tagccgccag
      901 tacaaaagta ccagcggcaa gatgcgtgag cgcagcgcgt tggcaaaaat actgtatatt
      961 ttgcgacgcg ctcggcacaa gaccgcgcaa gatttgatgc acgaaagcag cggcaacatg
     1021 ccgtactggg cggcagaaga ccccaccaaa ttttgggcgg cagcggataa gcacgagcgt
     1081 aaaaacggca cgctttttat tgagcatctg ctggtgttac caagggattt aacactggag
     1141 caacatattg agttggtgga aaggtgggct gagacagagg cctcccaccg ggcgtatacc
     1201 tatgcgattc attactcaca atcagcagat ggcggcatca atccgcactg tcattttgta
     1261 ctaaatgaaa gtttacaaga cggagtcaaa cgtggagaaa gtaagttttt caaacggtac
     1321 aacgcaaaaa aaccagagcg gggtggttgt aaaaaagggg ggacgggcgc acgaacccca
     1381 gccgaaaata agcaggtgtt gatacagcag cgccagctat ttgaagacct atgtaatgaa
     1441 gcattggaac gcgcacaaaa agacacgcgc gtatcgttac agagctataa aaatcgggga
     1501 ttagacattg tgccagagcc aaaagtcggg ccaaggggct ggcgtaatga gaagatacgc
     1561 aatgaaatat tggcgatgcg cgaggcaaaa aaagagttag ccaaggagtt aacaaccatt
     1621 caaaaaacgc aggtggttgc actggaacag tatcgtcaga taaaggaaaa acaggctgcg
     1681 ctagcacgcc aagaaaaagc gcaagaacga cttgctctgc agcagcgtga gagagaaaga
     1741 gagcaggaag cagtgaaggc gaaaaacgat ttttggcagc aagagcaggc cgagtatgag
     1801 cagtttgtgg caggagttga gcagcaggag gcagagatta ttgacttcat tgcattacag
     1861 gccgaagcaa cttaccaagc cagcaaagcc gattttatgc agctgtttgt gaatgagcta
     1921 acgcaggtag ccccgattta ttttgagtta gcacagcaag atttaatcgg gtttatgcac
     1981 tatgccgcta tacaagcgga tatggaacta gacgatgcgg tggttcgtgt ctttgtagag
     2041 aaagaaaagc cggtattgat ggcacagatt gcggagcgga tgcgggcgta tcgccaaggt
     2101 ttagtaaaac cccaagccct tatgtacttg atgcaggcca aggaaataaa ggagagtgtg
     2161 agctttgagc agttgattgc agagttagaa cagcaagccg tatacaagcc gccaaaaccg
     2221 gacgggtaag cgtgcctgac gtataaaaac ggtgacgata tcgcagtaaa gcgttacatt
     2281 agaagcaggc aaagaaaaag cccaactgcg acaacagtta ggctttgaag tttgatgact
     2341 ttcaccgaca aaagttagag tgcatcagtt ctatttattg tatgcactct agcgcaaatg
     2401 agcaagaata atgatgctag agcaaaaaaa actgtttatt gagaatttac cccacaagcc
     2461 gtattgcaca gataacctag attatgggtt gcagataagg tcggtaaaaa tcgccttaac
     2521 aaagcggctt attcagcata acaaaccctt tggtgtgtcg tggttggtgt ttgatttgga
     2581 ctcaccgcat ttttggagct tgttggaaag tgagacttta ccagcgccca atctggtttc
     2641 attcaatctc gaaaaccacc attgtcacat ttactacagc ctaaaaacac ccgtttgccg
     2701 ctcaaacgtg gcgaggcgta gacctttacg gtatttagcc gctattgagt atgccttacg
     2761 ggaaaagtgg actgctgatg caggatatac ggggtttatt agcaagaatc ccatccatcc
     2821 tcattgggag actcgtcaat ttcgtagtga gccgtgggag cttggcgaat tagccgactg
     2881 gctgacgcta cctaaaacct tacctagacg gggccagagt gttggcttag ggagaaactg
     2941 cacgcttttt gatatgttgc ggtactgggc ttatgacaat gttttagaat ttcgtattaa
     3001 tagtaatcgc aactcgtggt ctgacactgt tttaagcacc gcagagagct ttaatagctt
     3061 tcctgagccg ctacctacgg ctgaagtgag agcaacggct aaaagcgttt ctaattgggt
     3121 ttgggaaaac tacacaaagc gctggtctga tgaggagttt agtcaggtgc aggcagcgcg
     3181 gggcagaatg agcggagcaa agcgacgaca tgccagtaaa gaaaagcgag caaaggcgta
     3241 tgaactaagg gcggagggta tgacgcaaca agctatctcc aaggagttag gggtgaacca
     3301 atctacagtc ggtagatggt tgaaacgcta attatgcatg aaccatatca gataacagcc
     3361 cgtaggggct ttagctgtcc tgagtgtgtt tgataatggt ttatttgttg gggctttgct
     3421 tgtagaaata aataataaaa aaagcttacc ccctcctgtt gcctactatt ttctccctat
     3481 catcaagcac ggtttaaaaa acaagttacc cgtaccactt tggagcgtag cgaaaagtgg
     3541 ggcggaaaca agcgaagcgc gtagtgtgtg ct
//
LOCUS       DASRMS010000092         1174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_21443, whole genome shotgun sequence.
ACCESSION   DASRMS010000092 DASRMS010000000
VERSION     DASRMS010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1174
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21443"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(94..303)
                     /locus_tag="VFC74_04035"
     CDS             complement(94..303)
                     /locus_tag="VFC74_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005948602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin"
                     /protein_id="HZK24536.1"
                     /translation="MKQIKLCFKDFMRAALVRAVRTVAQTAVATIGAAAVISEVNWLA
                     TVSASVLAGILSILMSIATDLPEVE"
     gene            complement(318..626)
                     /locus_tag="VFC74_04040"
     CDS             complement(318..626)
                     /locus_tag="VFC74_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24537.1"
                     /translation="MADEKAMCIGHEQRISAVEESAKSAHKRLDELISLNDSVKNLAI
                     STAKMAEQMKTLTDNSAKLDRKISDLELKPARRWETVAMQIVQLLIAGVVGYLLSQLL
                     "
     gene            complement(631..>1174)
                     /locus_tag="VFC74_04045"
     CDS             complement(631..>1174)
                     /locus_tag="VFC74_04045"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HZK24538.1"
                     /translation="ATVLLELGLTTAALRTHNHWMGLPNKIVSGARKNCPYYILPHWD
                     KFVAKVANYVKEFTGKPDPKPEPTPATDVLYRVQVGAFKNKAYADKMLADLSNLKYFK
                     DNGIVPYISTAEDRKTTIARSVRITATRGLNIRDKPNGRKIGALNFGAVVKISEEKSG
                     WGKLADQDGWISTDYVESVR"
BASE COUNT          263 a          310 c          243 g          358 t
ORIGIN      
        1 acacataaga tgacggaacg gcagctatag ccgttccgtt gacttttcta atagggttat
       61 ccgccattgc gtcacctcat ctcagcttgc ctattactct acctctggca agtcggtagc
      121 tatactcatc aaaatagaca agatccctgc cagcactgac gcagatacag tcgccagcca
      181 attgacctcc gagatcaccg ctgccgcacc gattgtagct accgcagtct gtgctactgt
      241 cctcactgcc ctcactaggg ctgctctcat aaaatcctta aaacataatt ttatttgctt
      301 cataatttct cctcttttta taacaattgg cttagcaaat agcctacaac gcctgcaatt
      361 agcagctgta ctatttgcat tgcgactgtt tcccagcgtc tagccggctt cagctccaga
      421 tcgcttattt ttcgatccag cttggcgcta ttatctgtca gcgttttcat ttgttcagcc
      481 attttagccg tgctgatagc taggttttta acgctatcgt tcagtgagat cagctcgtcc
      541 aatcttttgt gagcggattt ggcagactct tcaacagctg aaatcctttg ctcgtgcccg
      601 atgcacattg ctttttcgtc tgccatcctc ttatctcacg ctttctacat agtccgtgct
      661 gatccaaccg tcttgatccg ccagcttacc ccagcctgat ttttcttccg atatttttac
      721 tacagcacca aaattaagag cgccgatttt tctgccgttc ggtttgtcac ggatgtttaa
      781 gcctcgtgtt gctgtgattc taaccgacct tgcgatagtg gttttcctgt cctcagcggt
      841 agaaatgtaa ggcacgatgc cattgtcctt aaaatatttc agatttgaca agtcagccag
      901 cattttatca gcgtaagcct tgttcttaaa agcgcctacc tgcacccgat acagcacgtc
      961 agtggcgggt gtcggctcgg gctttgggtc aggcttgccg gtgaactctt taacatagtt
     1021 ggctacctta gcaacgaact tatcccaatg aggcaagatg taatatgggc agtttttacg
     1081 tgcgccgcta acgatcttgt taggcaatcc catccaatgg ttatgagtgc gtaaggctgc
     1141 agtggtaaga cctagctcta gaagcactgt agcg
//
LOCUS       DASRMS010000093        48481 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_760380, whole genome shotgun sequence.
ACCESSION   DASRMS010000093 DASRMS010000000
VERSION     DASRMS010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 48481)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48481)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48481
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_760380"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1286)
                     /gene="tsaD"
                     /locus_tag="VFC74_04050"
     CDS             complement(261..1286)
                     /gene="tsaD"
                     /locus_tag="VFC74_04050"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HZK24539.1"
                     /translation="MTQKLILAIETSCDETAAAVVADGKIILSNIIASQVPVHQKFGG
                     VVPEIASRHHLEMINEVVEEALLEANCTFADLAAIAVTHGPGLVGALLVGVNTAKTLA
                     YALDKPLLAVNHVLSHIAANFLVHEVVFPAVCLVVSGGHTNLLYLTSRWQAELLGATR
                     DDAAGEAFDKVARVLGLGYPGGPAIDKAAETGNSSAFAFPRAFTGQNNNFEFSFSGLK
                     SAVINTLHNLKQKGENFQTADVAASFQQAVVDVLVEKTCLAAEVKQVKTVLLAGGVAA
                     NRSLRQQLEQALAAANRNLIYPPPVLCTDNAAMVAAAGWNLYVKQKFAGLDLNATPVL
                     DNLWQVQ"
     gene            complement(1283..1795)
                     /gene="rimI"
                     /locus_tag="VFC74_04055"
     CDS             complement(1283..1795)
                     /gene="rimI"
                     /locus_tag="VFC74_04055"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HZK24540.1"
                     /translation="MNVSIEPMTFFHVDKVSAIENEVYTSPWSVHAFINEILDNGFAS
                     YYVALLDEEVVGYAGIWVILDEAHITTLAVRPSFQGQGLGRQLLEHLIAEAAIKGAKR
                     MTLEVRVSNEIAQRLYKKYGFVACGVRPNYYPDEDALIMWLERLPVNTDKAETSGEQQ
                     AIKQVGEQKR"
     gene            complement(1792..2517)
                     /gene="tsaB"
                     /locus_tag="VFC74_04060"
     CDS             complement(1792..2517)
                     /gene="tsaB"
                     /locus_tag="VFC74_04060"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HZK24541.1"
                     /translation="MTIMYVMGIDTATLVCSVAVVSEEKTLAEYTQQVKKTHSERLLP
                     LIDTILRDTGLKPQDLSGVAVAGGPGSFTGLRIGMVTAKALGQALALPLCSISTLAAL
                     AAQHPLFPGLICPILDARREQVYNAIFTAGTQVERLVADRSLSVTELLQELQQRPEPV
                     LFVGDAVPVYRQIISRALVSRACFMPPESAICRASSVARLGLAQFSAGQVQSWRDLHP
                     AYLRRSEAEIKYQQTQIEGGDSC"
     gene            complement(2514..2978)
                     /gene="tsaE"
                     /locus_tag="VFC74_04065"
     CDS             complement(2514..2978)
                     /gene="tsaE"
                     /locus_tag="VFC74_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HZK24542.1"
                     /translation="MEYVINSAHEGETEALGIAVGRLLFPGAVIYLDGDLGAGKTVFA
                     RGIGQGLGVTTLVTSPTFTLLNAHQGRLPFYHFDLYRLQDESELFEIGLDECLDGDGV
                     SLVEWANKFAAYLASWPALTITISQDVTDRQLVLQAASKPYRQIVESLMAGR"
     gene            complement(3079..4080)
                     /gene="thiL"
                     /locus_tag="VFC74_04070"
     CDS             complement(3079..4080)
                     /gene="thiL"
                     /locus_tag="VFC74_04070"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HZK24543.1"
                     /translation="MKISELGEKALIKHISQIVAKTVTSEMVGIGDDAAITQVSQGSW
                     LVTSKDLLVEGVHFLLPGISAEDLGYKALAVNLSDLAAMGSLPRHVYVALALPAQLES
                     EFVLAFYQGLTALAERFGVAVSGGDTVASPGPLVISITAQGEVKREAALLRSGAQPGD
                     ILCTTGLLGASAAGLLLLQAEINCPLEWRQLALQAHFRPWPRVAEGLWLAQQGVVTAA
                     LDLSDGLLKDVQEICEASGCGVLLREEKVPIHPAVEAIAPLRQIAPITLALNGGEDYE
                     LLFTVKSDAYTALAKAYLERFQAPLYSLGEMIQERTLQMITKEGNWEKLEFTGYQHF"
     gene            4443..5588
                     /locus_tag="VFC74_04075"
     CDS             4443..5588
                     /locus_tag="VFC74_04075"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZK24544.1"
                     /translation="MKKSREIVGLPVVDLLAGSSVGRVHSLIIDPQTRHVAALEVGER
                     TLLKTNIELIPFSQLRNIGNDAITVISTETSSQSDKAATLENLAENQLSGTRVVTVDG
                     TFAGTVEDFSFSPTDGKLAELFIIQEKPRSHLACPVTVVETFGRDFIVVNENYQTAVR
                     KIIPPHPNNTPQHLVQTLESKAIDFALGREVRQEVLGENGDLIIRQGEAVTAENIALA
                     REKNRLPQLLFAAGVGELLDGLDFTREKLDAGSKKIMDAWQNLRGRSQVWLSRKLDDD
                     LGSTTTELRDLWQQVQGKLSQSGRELEDATRKQVRSYVLGKTLANPVYDPEGTLLGGR
                     GDLVTEEMRSKAETAGRLPQLFLSATTGDVQNVLEPIKKQIRNMLRE"
     gene            complement(5721..6530)
                     /gene="thiM"
                     /locus_tag="VFC74_04080"
     CDS             complement(5721..6530)
                     /gene="thiM"
                     /locus_tag="VFC74_04080"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF006830.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004417 - hydroxyethylthiazole kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HZK24545.1"
                     /translation="MDTGKIFTALRQRQPLIHHITNQVTISECANITLAAGGLPIMAH
                     AVEEVAEMVASADALVLNLGTLTPAQIEAMLLAGRRANELQRPIILDPVGVGATRLRS
                     ASAKKLLAALQLSVIKGNAAEIALLAGGAAKIKGVESIAVPSNVVPLAAALARETGAV
                     VVVTGAVDLVTDGQQLLEVHNGHPLMAKVVGTGCMTASVIGCFAAVERNYLVAAAAGL
                     AAINVAAEKAAAEADGPAMFKIKLFDQLAGLSVSTLAARQKIKNGLLEKWQ"
     regulatory      complement(6682..6796)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(6815..7924)
                     /gene="hisC"
                     /locus_tag="VFC74_04085"
     CDS             complement(6815..7924)
                     /gene="hisC"
                     /locus_tag="VFC74_04085"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HZK24546.1"
                     /translation="MRKQLHREKILAIKPYVPGKPVEEVERELGISDVIKLASNENPL
                     GPAPAAIRVLQGMLDNVSVYPDGNCYYLKTALAEKLGILPANLIVGNGSDEIIKMIAE
                     AFLDEGEEAIVADPTFSEYDFAVKVMGGRTVVVPTKDLTHDLTAMAEAINEKTKLVFI
                     CNPNNPTGTIVKKDAVQAFMARVPEDVIIVFDEAYYEYVRDEQYPQTLDYVRAGRNVI
                     VLRTFSKIYGLAGLRVGYGVSTPELIGMLSRVKEPFNVNLLSQAAALAALGDKEHLAD
                     SRQVNEEGKAYLYSEFSRLGLLYEPTEANFIWVEIKADCQKVFNKMLRRGVIVRTGDI
                     FGAPDVLRITIGTAEQNKRLISTLEAVLTEEGVLG"
     gene            complement(7936..9231)
                     /locus_tag="VFC74_04090"
     CDS             complement(7936..9231)
                     /locus_tag="VFC74_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HZK24547.1"
                     /translation="MYWNQEAECMSRDRLQQLQLKRLKKTLERVLSVPHYAQVFQTAG
                     LEPGDINELADLSELPFTVKQDFRDTYPFGLFAVPQNEIVRYHSSSGTTGKPIVVGYT
                     RHDITTWAELMARALTSAGTTRNSVVQNAYGYGLFTGGLGVHYGAELVGAAVVPASGG
                     NTQRQIMLMKDFGTSILTCTPSYALYLAEVMSEMGLVPDDLQLKAGLFGAEPWSEAMR
                     QQIESRLGITAMDIYGLSEIIGPGVAMECDEKHGLHIFEDHFIPEIINPASGEVLPYG
                     EEGELVLTTITKEALPVIRYRTRDLTTLTLEPCLCGRTHVRMGKITGRSDDMIIVRGV
                     NIFPTQVESVLLEVGETEPHYQLVVTRQGSLDLLEIQVEVSESMFSDKIKRLEELEQK
                     IHSKIDSVLGISAKIRLVEPKSIPRSEGKAKRVIDKRQI"
     gene            9419..10189
                     /locus_tag="VFC74_04095"
     CDS             9419..10189
                     /locus_tag="VFC74_04095"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK24548.1"
                     /translation="MIVSFVLSRKRKRIILLVIAALVLLCLVKIFWANQTSATVGRKR
                     LVPIYYVDTTEQKIAISFDASWGAEFTPKILEILRENKLKTTFFLTGFWIDKYPELVK
                     QIADEGHELGNHTATHPHLNSLDKAAITKELTAVHNSLQELSGQKAILFRPPFGEYSD
                     KVIEACDDFGYKTIQWSVDSLDWKEISKEDIVARVTSQLHPGAIVLFHNNGRYTAEAL
                     PEIISYAQENGYEIVQISQLLYQDDYYIDANDGAQRKN"
     gene            complement(10251..11648)
                     /locus_tag="VFC74_04100"
     CDS             complement(10251..11648)
                     /locus_tag="VFC74_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24549.1"
                     /translation="MTKYVMSIATVLLIFTLCTAVFANSGPVYWQGYPAAEMMSIATD
                     TPLTVESEELVFDFSQKHTSSYALEGKVTATYQMSNPTSEQQAVQMAFPFVGQLRQLN
                     LAEIVIKADAQQLPYELYLGSRAEFNSRVSATDVVPSFAFTNILSALSRQQYSAAHFS
                     AEEIGRLYRFDVSPTTEQNINFVVTFNDNAENTKILINGFNGFAREAGKVRLSAWCSE
                     PQQFEVFALGEELEFTMQAYTNGELSEKTDLYTAQVTTQEQQVKLYLVSLSQHHLNTE
                     AAALLDDTQVYNLYAEELDQQFTQNLGVCALEELWALAEQERIMVLLYSLEFPPQRAQ
                     EISVSYKTSGTMDKRETVSPQYSFTYLLNPAAHWSEFKDLQIKIITSPEAPYLINSSL
                     PLKQTADRQYSADFTGLPGQDLTFTLFAAEKVTLADKVAGRVNRTFGYITPISLGILL
                     LLIILFGKKRTNSYF"
     gene            complement(11703..11987)
                     /locus_tag="VFC74_04105"
     CDS             complement(11703..11987)
                     /locus_tag="VFC74_04105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK24550.1"
                     /translation="MRGAFQSCHLGYKLSEAQINQGLLTEALAEVINYAFTELRLHRI
                     EANIMPGNKRSLWVVEKLGFYYEGLAKKYLKINGSWEDHIHLVLRSQALE"
     gene            complement(12006..12278)
                     /locus_tag="VFC74_04110"
     CDS             complement(12006..12278)
                     /locus_tag="VFC74_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24551.1"
                     /translation="MQFKGPLLVVKDINLATSRLDLRVLVAAEQVLAYYQRNQEFLAP
                     WEAVKEEEFFTLTYQQDQLQNELHSMREGRLFKVWLFFNEGQEIIG"
     misc_binding    complement(12559..12805)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(12906..14405)
                     /gene="spoIIP"
                     /locus_tag="VFC74_04115"
     CDS             complement(12906..14405)
                     /gene="spoIIP"
                     /locus_tag="VFC74_04115"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HZK24552.1"
                     /translation="MKRKILVLLFVVTLCSFFMLTSNELTTTSRSLFDFFDNQDTGEI
                     SREDLLAENYYRMVAEDGKEIMVTGRKIHIGDEYLTSNNQLYRVYRVRNYVAEARFIR
                     EVGAIFAEEPQSLLVRLQQRFFGAVQPVQKQEKSKTEKDKQEKEKTKNKKDQKDKKNE
                     DLQPQAEPKRLIGVYHTHNAESYVPTDGTDSIVGKGGIHKVGDSFTKALQEKDIKVIR
                     SNTLHLPHDRGAYRRSRVTAEEILKKGPDVIFDVHRDAGPANVYAAEVAGEAVTQVQF
                     VVGRQNPQMQTVRQFALDLKNTADEIHPNLVKGIFMARGNYNQDLTATSMLIEVGTHL
                     NQREKAQDGAALFADVVSYYFYGPEDEDDDNNERAAPAPLQSPASKGGGGAGNVGRAA
                     ARNAFWMFAVSAVLAAGFYFLNTPLGAIREKLAPWFSKALPYTEKGDIFLASVQEKIR
                     HTAILISDTTMDLLRNGDQYTASWQERIQDFVQTIKEKAEQLRNRDKLS"
     gene            complement(14402..15031)
                     /locus_tag="VFC74_04120"
     CDS             complement(14402..15031)
                     /locus_tag="VFC74_04120"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZK24553.1"
                     /translation="MFTTNVGTLSLIVVTVLIYLGFLHRVLDRMRLTKIQALVILLAM
                     LAAGFLPNIPIYAGLSLNIGGALIPVGVAVYLIVTANEAAEKRRALITAGVVSVLVYL
                     TEKLLPMEPTSAAFLVDPLFMPAIVAAVTAYLLGRSRRSAFIGGIFGVILTDLFAWLE
                     TLFLYQLHVPIVLGSTGVYGAAVIAGMGAVLLAELVGEILERLQGGHRV"
     gene            15223..15651
                     /locus_tag="VFC74_04125"
     CDS             15223..15651
                     /locus_tag="VFC74_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24554.1"
                     /translation="MLRSIVNKMKSAAHFLNYPILQYIPISMHSLLQKDNWHNPFSQA
                     TPQLLLFQNKAAFESALQHYQVQLSAPIPAQELYVICLNFKSGLVLFRYLTCKFVGTP
                     QLGGIHIFAVTKKYFPPRAIHFALYTEDGRKLRSMTQKII"
     gene            complement(15680..16390)
                     /locus_tag="VFC74_04130"
     CDS             complement(15680..16390)
                     /locus_tag="VFC74_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HZK24555.1"
                     /translation="MTIRIGITCNSVGGESRLSQMYVRAVAATGAVPLLLPVCSQVGS
                     YQQMLSTVDGLLLSGGGDPDAYHFGEQAAPQQGLVQPERDRMELYLTRAALKKGLPLL
                     GICRGAQILAIAAGGKLYQDLAAREKVQHQQQAPRSYPIHRVSVSKNSLLYQITGKQS
                     FRVNSLHHQAVRKLGAGMKVCAVADDGIIEAVELPSHRFVLGVQWHPEWLSHKYGHAA
                     AIFAAFSQAAGHEQQGRK"
     gene            complement(16459..16809)
                     /locus_tag="VFC74_04135"
     CDS             complement(16459..16809)
                     /locus_tag="VFC74_04135"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_388347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HZK24556.1"
                     /translation="MEVKRGYIFFADLSPVIGSEQGGVRPVLVIQNDVGNKYSPTVII
                     AAITSQIDKAKLPTHVEAAAKEYGLEKDSVILLEQIRTIDKQRLQDKVTELDERVMQK
                     VNQALKISLGLIDF"
     gene            complement(16815..17087)
                     /locus_tag="VFC74_04140"
     CDS             complement(16815..17087)
                     /locus_tag="VFC74_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HZK24557.1"
                     /translation="MAETKRIMISLPYNLLEEVDGIVATEKRTRSDFIREAMRLYLAE
                     REKQQIREKMQKGYAEMAKLNLRLANEAFDVENEANLIAELLVGKK"
     gene            complement(17100..18284)
                     /gene="alr"
                     /locus_tag="VFC74_04145"
     CDS             complement(17100..18284)
                     /gene="alr"
                     /locus_tag="VFC74_04145"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HZK24558.1"
                     /translation="MEYNINCKDRWCSVLCGLPLRPTWAEIDLGAIRHNIQQFRLHIS
                     SRTQLMAIVKADGYGHGAVQVARTAVAAGVSFLCVGMVEEAIELRENGLTTPILILGF
                     TPQEYASYLVQYNLTPSVFTLAEAQAFSRAAVQSATRLDVHIKVDTGMARVGCQAGEA
                     DSFVQQIAALPGLQVAGLYTHFASADQADLTFARLQLSRYLALVQRLEAQGIMIPFKH
                     VANSAAAICMPEAQLDIVRLGIGLYGLYPSAEVSQKQIQLRPAMTLKAKIIYIKDVPA
                     GVGVSYGSTFVTTQPTRIATLNIGYGDGYRRQLSNLGQVLVHGKRVPIVGRICMDQTM
                     INVQDVAGVQAGDEVVVFGRQNGAQLPADEVASWLDTINYEVVTAISRRVPRIYLGED
                     EI"
     gene            complement(18333..19562)
                     /locus_tag="VFC74_04150"
     CDS             complement(18333..19562)
                     /locus_tag="VFC74_04150"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HZK24559.1"
                     /translation="MVWIVQKYGGTSVGSAEKILNVARKIVRTVAAGHGVAVVVSAMG
                     DTTDDLTALAKQISKQPSERELARLLVTGEQVSSTLLTMALHSLGQAAVSLTGAEAGV
                     LTKGNPLNARICDFKADAVRKALAAGEVAVVAGFQGIDASGEVNTLGRGGSDTTAVAL
                     AAAIGAESCEIYTDVDGIYTIDPRLVPTARKLTAITYDEMLELASLGAKVLHPRAVEC
                     GKEHHVVIHVRSSFHDQVGTLVKGDIGLEKGTVINGIACDENQVKFAVMGVPDRPGIA
                     ARIFNAVAQAGLNVDLIVQSIPREGENDILFTLAGDDFEQAHTILQPLVEEVGARALR
                     YQRNVAKISVVGAGMIHHTGVAAGMFQALAEAGINIEIISTSEIKISCLIAADRLHEA
                     AQAIHSYFNLQERCECP"
     gene            complement(19590..20879)
                     /locus_tag="VFC74_04155"
     CDS             complement(19590..20879)
                     /locus_tag="VFC74_04155"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HZK24560.1"
                     /translation="MSKETIKIGVLGMGTVGTGVVEILTGNSNNIAHKLGVNLEIKRI
                     AVRDVQKPRALNLDRALFTDNIDHVLLDPEIDIVVETMGGIEGARQAITRALENGKFV
                     VTANKDLMAHHGVELLAIAKRQGKNVYYEASVGGGIPLVRPLKYSLAANRIKKFMGII
                     NGTTNYILTQMSASGKEFAEALAEAQAQGFAEADPSSDIEGRDASYKLSILASLSFSS
                     RINIEDVFVEGISKVKLRDIHYAQELGYAVKLLAIGEEVTEGLKLHVHPTLIPLEHPL
                     AAVKNEFNALFLEGDAVGDVMFYGRGAGSLPTGSAVCADVMDAARALKNEIQNGIVEA
                     TFTEKHIVPMAEQYSRFYLRLKAEDEPGVFAKLATAFGDAAVSLDRIIQKRSVEGTAE
                     IVLVTHDVREGNFKQALNSIQAMASIRSINSVLRVVE"
     gene            complement(20902..21348)
                     /locus_tag="VFC74_04160"
     CDS             complement(20902..21348)
                     /locus_tag="VFC74_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZK24561.1"
                     /translation="MQNSDDRLYLVQEHVLPQVLRKTAEAKELLKNGKAKTINEAVQL
                     VGISRGAFYKYRDNIFPFREASRGKILTISLILEHTAGVLSDVLKAIATAGGNVLTIN
                     QGIPLQGVANASISFETDGLSEDVNLLLTRLAAIPGVQKLELVGQS"
     gene            complement(21554..21775)
                     /locus_tag="VFC74_04165"
     CDS             complement(21554..21775)
                     /locus_tag="VFC74_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZK24562.1"
                     /translation="MGLDEFPGGPGPAPNPEKLPVCPSGLFLEIVPGDTLFKIAQEQG
                     VTVEGILALNPGINPKNLQIGTFICLPTP"
     gene            complement(21824..22846)
                     /locus_tag="VFC74_04170"
     CDS             complement(21824..22846)
                     /locus_tag="VFC74_04170"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4367 domain-containing protein"
                     /protein_id="HZK24563.1"
                     /translation="MIKRSLAGLLVLTLLLSGCAVTSEKLLTQIEKRVEKLDGFYAEL
                     EVLVFSLGGEQSYQVKQWQTADNRWRVEVKAAAEAQHFICDGQQIWVYQPGLGDYYRI
                     DAERGALELAPPFMLTGYLQRLQQADSFTFKGEQDYQGEKMYNLSCAGQLPGETLQLW
                     LDKKTFFPMLIETYLDEQLLNRITVKKLELNPPLPAELFKFSGEAESEVTTQCQREPL
                     TLAEAREGWPGSIFVPTYLPTGSSLFVVTRTMEENREHLILVYQGHKNFTLIQQARAD
                     ENIYCSAKTSKVQVGQRTGLYQQNSDRSLGTLWWSNEQSDFVLTGALPLSEMIEIAES
                     LKPEGV"
     gene            complement(22924..23370)
                     /locus_tag="VFC74_04175"
     CDS             complement(22924..23370)
                     /locus_tag="VFC74_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK24564.1"
                     /translation="MSIKEIMTSEVIAVTQETTVDEAARLLLEHKISGLPVVDEQQQV
                     VGMVTEADLIYQAKELHTPAFTEILGGVIFLEDPDNFKKQLQKMSGYRVADVMTTPVF
                     TVAADASKEEVATMMVEQGINRVPVVGTDGRLQGIVSRQDLLSAMF"
     gene            complement(23386..25077)
                     /locus_tag="VFC74_04180"
     CDS             complement(23386..25077)
                     /locus_tag="VFC74_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HZK24565.1"
                     /translation="MVVEIAAVTVVKKKAHLLRLFSDSGDGSLSRSPHTTASVNIITG
                     YEDKEVGEMNVVTAAEMRAIDQKAIAEYGIPGIVLMENAGLRLVEAIRQGQYGERIMI
                     IAGCGNNGGDGFVAARHLSAEKDVSVWITAHPEEYSGDALLNLKILEKLGMTVHCLRD
                     KTVWRDFSLALPRANLVVDAIFGTGLSRPLDEFYQQLIQQLNECASAVMAVDLPSGIN
                     ADTGHDLGAAVRADVTVTFALPKLGHFLYPGAAKRGRLQVVNIGIPPALLSGYSHTLL
                     TSTTVHLPEREANSHKGTFGTVLLVAGSPGFSGAAVLSAAAALRGGCGLVQLAIPRSI
                     HSMVAAQAVEAITVSLPVDQTGQLQATALTMLQEKWPQCHAVAIGPGLTQREQLLPLL
                     AGLLKECNLPVVLDADALNLLTLAPELLRERQAPTILTPHPGEAARLLGQTTQEVQTD
                     RLAAAQYLAKHFRSTVVLKGAHTIVADPDGAAAINSSGNNGMATAGSGDVLTGLLVSL
                     VAQGLPAAQAARLAVYWHGLAGDLAAHALGKASLVARDLLDYLPQAYLEISKIDI"
     gene            complement(25043..25417)
                     /locus_tag="VFC74_04185"
     CDS             complement(25043..25417)
                     /locus_tag="VFC74_04185"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HZK24566.1"
                     /translation="MHPLELAEQLGESILASDEYQSFTHAQEIMEQSDAAVSLIENFQ
                     AQQLALNNKQSQGQSITPELVEELQTLQRQMVENQEIKAYLIARQKVDSLLAAVNETI
                     ARITGMETGRSGGCGDCSGDCG"
     gene            complement(25438..25818)
                     /gene="nifU"
                     /locus_tag="VFC74_04190"
     CDS             complement(25438..25818)
                     /gene="nifU"
                     /locus_tag="VFC74_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014828336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold protein NifU"
                     /protein_id="HZK24567.1"
                     /translation="MYTEQVMDHFTNPRNVGEIENASGVGEVGNMKCGDIMRIYLKVN
                     EQDVIEEIKFRTFGCGAAIATSSKITEMVKGKTITEALKITNKQVAEELGGLPQPKMH
                     CSNLAADALRQAIKNYQQQTDKHK"
     gene            complement(25837..27000)
                     /gene="nifS"
                     /locus_tag="VFC74_04195"
     CDS             complement(25837..27000)
                     /gene="nifS"
                     /locus_tag="VFC74_04195"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase NifS"
                     /protein_id="HZK24568.1"
                     /translation="MRKVYLDHAATTPVHEEVFAAMKPYLTEHFGNPSSVYAIGREAR
                     KAVDEAREKAATALGADPQEIIFTSGATESNNLAIRGIARAQQHKGKHIITSAVEHHA
                     VLDTCLALQKEGFEVTILPVDEYGLVSKKDVTEALQEQTILVTVMMANNEVGTILPVA
                     EIGKLCRERGIVFHTDAVQAVGKLPIDVKELQVDLLSLSAHKFYGPKGIGALYVRKGT
                     GLNIYSFGGAQERKLRPGTENVPGIVGLGRAIELAVSKMAEKNKRVQCLRDKLKAGVL
                     AIEDTRLNGHPEQRLPGNLNVSILYVEGESLILSLDLKGIAVSSGSACTSGSLDPSHV
                     LMAMGLDHQTAHGSLRFTLGDDNTEEEIDYVLEVLPEIVARLRSMSPVYAGKK"
     gene            complement(27017..27433)
                     /locus_tag="VFC74_04200"
     CDS             complement(27017..27433)
                     /locus_tag="VFC74_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZK24569.1"
                     /translation="MKVSAKGKYGVRAMAILALEFHEGPVPLREIAEREDISFQFLEQ
                     IFQPLRRAGLIDSVRGAKGGYVLAKAPDKVKVGDVLRALEGPIAPVECVIEGNNNACG
                     RSAACLTRVVWERLRDSMSEVLDDITLADVIKMSAE"
     gene            complement(27532..27930)
                     /gene="acpS"
                     /locus_tag="VFC74_04205"
     CDS             complement(27532..27930)
                     /gene="acpS"
                     /locus_tag="VFC74_04205"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HZK24570.1"
                     /translation="MNKESLALHTGVDIIEIERVARAYRRRPQLFLQRFFTAQEQEQL
                     AARSYGARHLAARFAAKEAVFKLLGCGLGSIAWTDVEIISLPSGQPTVCLRGRAKLRA
                     AKLSLHSIALSLSHSRNYAVAQAVALAMTK"
     gene            complement(27958..28665)
                     /locus_tag="VFC74_04210"
     CDS             complement(27958..28665)
                     /locus_tag="VFC74_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK24571.1"
                     /translation="MSKVTKILVVDDEPKIRRIVQQSLRQDAYRVIHAADGREGLQKV
                     ADEHPDLIVLDLMMPEMDGFEVCKALRSRGDNTPVIILSAKDELEDKAQGFNLGVDDY
                     VTKPFSPSELALRVKAVLRRVAGEELAPRLKEGTFDDGRLSINSKTREVALKGEHVYL
                     TAKEFDLLWVLANNPGVVYSRDQLLDLVWGNEYFGDTGTVTVLIRRIREKIEKDPANP
                     TYLRTVWGIGYKFETVL"
     gene            complement(28649..30262)
                     /locus_tag="VFC74_04215"
     CDS             complement(28649..30262)
                     /locus_tag="VFC74_04215"
                     /inference="COORDINATES: protein motif:HMM:NF028468.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE3 domain-containing protein"
                     /protein_id="HZK24572.1"
                     /translation="MTKLSMESPYLKIIAAVLLAAALIGLSFRNYLLFHTVVEIFSIL
                     VSFGIAAIAMHTSRISYKNDHLLNFLGFIYFFVAVFDLMHTLAYQGMGVFAGDTANLA
                     TQLWIIPRFFEAFALLLSAALLSKALTTPKVLIPIALLAISLFVMVFTGYFPACFIPG
                     VGLTTFKIVSEYLIVFIILLAIGFLIKNGRRLDRDLYQYLLLACLATVFAEISFTFYS
                     SVYGLANMVGHLWKLLSYYFIYTVIIRKKLLGPYDTIKDLYQNMERKVATRTRELEAA
                     NLRLREGQRVLTQLNQKLSETNRLKSEFLATITHELRTPLTSIVAFCELLLDETAGPI
                     NEEQRENLLDIQIGSQQLMILISDILDMAKYEAGHLRLEPENLDLNDVLYIVRRTMVP
                     VAAQQNITLEVQKAELPLVYADPERIRQMIINIISNAIKFTKEGGRVTVFTTAEDARA
                     AIHIEDTGEGIAPELLPHIFEKFRQGDSSLKRRRNGTGLGLALVKTLAELQSGNVSVQ
                     SEIGIGTKFSIYIPFSRTDQGGDNDEQSY"
     gene            complement(30441..31397)
                     /locus_tag="VFC74_04220"
     CDS             complement(30441..31397)
                     /locus_tag="VFC74_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24573.1"
                     /translation="MKIRSVLLLVLILLLSGCGTEADLEPVVENFEPADKIVSLVDVD
                     FTTVPDFTYHILLEEKMSPNKKQFIRRYVRYNGIKNTATEVLQEVALPERQHDVNGAK
                     NWLQENDPDKLVVYCQTYLSDSVQAQWEDSDYDFYIEEAQLISLEGLKKLGAISRLNE
                     IFVINKKPEEMVELIEAVEADFLKPAPNLLIERELEALPRSFSYQLQLQLGTEPTKKP
                     STILCTAKIILMYQIDELGNPIELNFEASFEDHTKQGSQSWLPGTNQVGIFMVEMRNS
                     LISRLDLFGVTPYNPTLAYANVTLNYEGIAHDNLLTERIYIP"
     gene            complement(31517..32605)
                     /locus_tag="VFC74_04225"
     CDS             complement(31517..32605)
                     /locus_tag="VFC74_04225"
                     /inference="COORDINATES: protein motif:HMM:NF008519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HZK24574.1"
                     /translation="MYDIAIIGAGPAGSTLARLLAAKYRVLLVERRNLLVPVQAAAGT
                     KACGGLLAPDAQKVLGMMSLALPQEVIVGPQIFVVRTIDLQQGRERYYQRFYINLDRT
                     KFDHWLFSLVPATVEVRADALCKDIQQEDNHITLDLLQQGKHYQEKARLVIGADGAGS
                     LVRRKFFPNDQKLVIYSAIQEWFSVKEASPYFSVFFDSELMDFYGWSIPKEDSLLVGA
                     ALRPRQQTVERFKQLKAKLVAYGYPLQTAQKKEGALIVRPRHAPRLVALPPGVALIGE
                     AAGWISPSSAEGFSYAFRSALAAAKSLAPGLDGFVARYQRETAVLRRNIVLKNLKLPF
                     MYHPWLRGAVMSSGLAAVDVMKNEELKS"
     gene            complement(32689..33339)
                     /locus_tag="VFC74_04230"
     CDS             complement(32689..33339)
                     /locus_tag="VFC74_04230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006717966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HZK24575.1"
                     /translation="MYKHILFDLDGTLTDPAAGIISSLRHALAQMGVDEEDEEKFRLF
                     IGPPLKDSFRTMYAFSEEQVDQAIAIYREHHGVSGLYENKVFPGIREMLADLRADGRK
                     LYVATTKMTAFAELILKHFALDTYFTKIVGGHPDGTRTAKAEIIADILQDIPQAERQA
                     VVMVGDRMFDVVGANAQGIDAIAVTYGYGQEEELQAENPTHIAHSVAELVTILRQV"
     gene            complement(33363..34505)
                     /locus_tag="VFC74_04235"
     CDS             complement(33363..34505)
                     /locus_tag="VFC74_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HZK24576.1"
                     /translation="MAKIELIATAAFGLEAIVAKELKALGYRDQMVENGRVTFYGDEE
                     AICRTNLWLRSADRVLVKVGEFIATTFEQLFEQTKALPWQEWLPQDAEFPVDGKSIKS
                     KLFSVSDCQAIVKKAIVEKLKQNYQQTWFEETGPKYKVEVALLKDRATLTIDTSGAGL
                     HKRGYRELTAKAPLRETLAAALVRLSKWPADRVLADPLCGSGTIPIEAALLGLNIAPG
                     LKRHFSAEKWPNIPHHLWQTARTEAQDLIVRDRTLRILGTDHDDQVLSLARHHARKAG
                     VESHIHFQRLALTDFRSRFDYGYVICNPPYGERLGDVQEAEALYREMGQVFGQLPTWS
                     FFVITSHKGFEKIFGRPANKKRKLYNGRIECHYYQYHGPNPPKQRD"
     gene            complement(34852..35229)
                     /locus_tag="VFC74_04240"
     CDS             complement(34852..35229)
                     /locus_tag="VFC74_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZK24577.1"
                     /translation="MLRKVLLFFLIALLIVGGGMFFAFWRGIQQINDFVFADVNLANI
                     ADGVYQGDVAAGPIQVIVDVIVQDAQIAEIKLIKHDHGKGEAAESIVDEIITQQSLQV
                     DTVSGATYSSRVILKAVESALLH"
     gene            complement(35434..36369)
                     /locus_tag="VFC74_04245"
     CDS             complement(35434..36369)
                     /locus_tag="VFC74_04245"
                     /inference="COORDINATES: protein motif:HMM:NF022112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HZK24578.1"
                     /translation="MMRKLVWVLVLLLLLCGCAQNKESPPIEPEEITANVTLYYGDAN
                     NEKLVSEERQITFQEEEDRYKAALQALIAGPETAGFTRNIPAGTKVYGTMRQDNALLV
                     NLTEHFLSFGGSVAEIIAVASVVNTLTESAEIEKVKILIEGSELIGPSGEARGFMTHF
                     TADGGSPTAEEEVTLYFANPQATALIPEKRTLSFALSADLADRLRIVLAELIRGPQGN
                     NLAPTIPAEVQVHSVSMQDGIAVVDFSPEMHTKHSGGAAGETMTITSIVNTLTEFEGV
                     DLVAITVDGKPLNIEHVILDAPVERNEEIVEEQSE"
     gene            complement(36539..37045)
                     /locus_tag="VFC74_04250"
     CDS             complement(36539..37045)
                     /locus_tag="VFC74_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HZK24579.1"
                     /translation="MKNILTRRSIRKFTEQGLTTEQLTALLGAAMSAPTGRNARPWQF
                     IVVRERAILAGMRDCAPNTAVLAGAPAAIVVCGDLEKELVPGLWVQDCAAATENLLLA
                     AHASGLGAVWLSIYPNDEKVGKLRTYLGMPEHVIPLAVVALGYPAEEKAVGDRYDPQC
                     VHYDRWEL"
     gene            complement(37076..37396)
                     /locus_tag="VFC74_04255"
     CDS             complement(37076..37396)
                     /locus_tag="VFC74_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012102779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative heavy metal-binding protein"
                     /protein_id="HZK24580.1"
                     /translation="MLVTTTPIIEGRKILEYRGIMFGEVISGVDFIKDFSAGLRNFFG
                     GRAGAYEGELIEAREMAIEEMVKRAAAVGANAVVGVDIDYEVLGQGGNMLMVTASGTA
                     VVIE"
     gene            complement(37515..37832)
                     /locus_tag="VFC74_04260"
     CDS             complement(37515..37832)
                     /locus_tag="VFC74_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR04076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0046500 - S-adenosylmethionine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HZK24581.1"
                     /translation="MTEEKKAKVRITILKKTKVEDVHSAYAKETVPVVCAKGEEGLSY
                     LAVNGEMPADFCPGAWRGLAATVELLAAGGTSPYTREEGIAISSCNDGLHPVIFKLER
                     VAS"
     gene            complement(37967..38665)
                     /locus_tag="VFC74_04265"
     CDS             complement(37967..38665)
                     /locus_tag="VFC74_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24582.1"
                     /translation="MNNLGILIVWPSILILLGIFSGIARTARVSFRQTRITVSLYLAV
                     LLIFTGAFYLVADAKEAASAQDIQVAEAEAEAMQLQFYAALEKGRLSKLEGIQINGYW
                     NFTYYDDVLYLTTAADQYFEPLVAIERKEINDGKIDVTSYTTSLKFKGDLLGNSPSVR
                     RIDNKLELTNAAQFEMVAFAFTKDWTMQQFSDSSNNWHWNMSSLLGAQAVVIQVPKDL
                     QVEVEVEQGLNLVP"
     gene            complement(38662..39405)
                     /locus_tag="VFC74_04270"
     CDS             complement(38662..39405)
                     /locus_tag="VFC74_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24583.1"
                     /translation="MSFHEPKLLAVCKKQYAYKLKTNLGIFFSLMIVQLLALLLSLNG
                     SASMGAGSSSMVINLKLISGDIIIVFTLIWAFIIGNTVAGNACRLDFSFVSTRLTSNL
                     ASVCLLATAALVGGITATLSGVLLRLLVYWFQDVYTNNSLYLAPLTLLSGSYVTALYA
                     FLLSVLGYFFGMLTQRNKALTIVIPVLFLGSLFIEARRAGEVQLLTTIFVFFVRETSM
                     LLFSMKVVCAAALLFATVILFTNRMEVRR"
     gene            complement(39386..40288)
                     /locus_tag="VFC74_04275"
     CDS             complement(39386..40288)
                     /locus_tag="VFC74_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009622461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24584.1"
                     /translation="MICIESESLAKSFGKFKAVNNLSFSIEENKITGLVGRNGAGKTT
                     LLKLLAGYLLPSSGEVRVFGQTPFNNLHVAANLIFIDDQLELPDNLNLGETLAAIGQF
                     YANWRAELARGLLDYFSLQPQQYYANLSKGMKSSLKMIIGIAARCPLTIFDEPTVGMD
                     VAVRKDFYRALLKDFMQYPRTIIFSSHLLSEIEELLDDLLLIKAGEKYLHLPLDELKG
                     YAVGFTGQASIINKMITPEQILYQRQLGKDSIYLVARNTFNQEILQQARLQGVEISPV
                     ATDDLSIYLTAQHKGGIDNVFSRT"
     gene            complement(40291..40674)
                     /locus_tag="VFC74_04280"
     CDS             complement(40291..40674)
                     /locus_tag="VFC74_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007783324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK24585.1"
                     /translation="MLLNPDSLKPIYMQIAEWLELEILRGSIASDEKIYSQYQLAEMF
                     NINPATAAKGLSMLVEENLLYKKRGLGMFVAPQAKERIRTKRINESLQQLVLEVVAEA
                     GRLHVPREELVRMIEMAEQSFAEGE"
     gene            complement(40823..40975)
                     /locus_tag="VFC74_04285"
     CDS             complement(40823..40975)
                     /locus_tag="VFC74_04285"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HZK24586.1"
                     /translation="MYEELQQKVERERQRLAAIINRFGINHNQTIKQSEKLDKLIVDY
                     LRETNK"
     gene            complement(41185..41901)
                     /locus_tag="VFC74_04290"
     CDS             complement(41185..41901)
                     /locus_tag="VFC74_04290"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZK24587.1"
                     /translation="MDGVSILALVDIISEIIKVPLLLLDKQGEIIYASPYFNNQGKQE
                     LLLRFYQAFVINSGTLISSQADLDGVEFIYTKIRIKKEPFYLFTGHFIKPKQEQQLES
                     ACGQEMGQLLLTHNLYPNGQRQLTQLQTMTSILGELIVREYQIKELSRQLNSMTTEQL
                     TASIEESLMHLEPANKLTRLTRRQLEVLKKLAEAKTNRQIAAELQISEKTIKVHVSNI
                     LHELAVKDRMEAVLYYLAKK"
     gene            complement(42101..42679)
                     /locus_tag="VFC74_04295"
     CDS             complement(42101..42679)
                     /locus_tag="VFC74_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HZK24588.1"
                     /translation="MTTVTPTREATYNLLTEYNKSDSLIKHALTVEGVMVHFAELFNE
                     DQEKWGIIGLIHDLDYEMYPEQHCQKTEEILRANNWPEDYIHAVISHGWKLCTEVEPQ
                     ERMEKVLYTIDELTGLITATALMRPSKSIFDTNLKSVKKKWKQKSFAAGVNREVVEEG
                     AKLLGMELDDVMMETLKGMQKVGESIGLQGNS"
     gene            43104..43544
                     /locus_tag="VFC74_04300"
     CDS             43104..43544
                     /locus_tag="VFC74_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24589.1"
                     /translation="MKIGNRTQQGLLAGLFAGIVMDITDQLTYLLVKEHTLRSADWVA
                     LLIYGRQSNHDLELLLAQVIHFLFTAMLGLLYVYFIAPIKIKNHLFKGWLWSTFVWFV
                     AITLTISTKIPALTEIDVNDFIFHFLLASIYGLTLAAANARLAK"
     gene            complement(43568..43930)
                     /locus_tag="VFC74_04305"
     CDS             complement(43568..43930)
                     /locus_tag="VFC74_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008398525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HZK24590.1"
                     /translation="MGKNIKVKPGKGQSTIGFFAGLVFCGIGFFIVIPTAGLFGIFWT
                     IMAVIITVVNGINAFTNKAIVSHEIMISDAPELQVTNNEERLEPEQRLQELQNLYDKG
                     LISATEFQQKRKNIIDSI"
     gene            complement(44116..44841)
                     /gene="nagB"
                     /locus_tag="VFC74_04310"
     CDS             complement(44116..44841)
                     /gene="nagB"
                     /locus_tag="VFC74_04310"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010963511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006046 - N-acetylglucosamine catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HZK24591.1"
                     /translation="MRIYVVDNYEELSVKAALIVAGQVALKANSMLGLCTGSTPIGMY
                     KELVKMHQQQALDFSQIISFNLDEYFGLSPESEQSYHYFMQDNLFKHLNVTQENINIP
                     NGLAAKTDEECLRYEQAIAQAGGLDLQVLGIGRNGHIGFNEPAPEFIGNTHLVQLDED
                     TIYANARFFDSRTEVPTQAITMGIKTIMQAQKILLLANGAGKAQAVKDTILGEINPHV
                     PASILQLHDDCTFIIDKEAAQYL"
     gene            complement(45085..46428)
                     /gene="glmM"
                     /locus_tag="VFC74_04315"
     CDS             complement(45085..46428)
                     /gene="glmM"
                     /locus_tag="VFC74_04315"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HZK24592.1"
                     /translation="MGKLFGTDGIRGVANKELTPELAFRIGRIGAYLLQQKSKGHKAF
                     LIARDTRVSGQMLEGALIAGITSAGVDVHVAGVISTPAAAYLTKQLNGCGGVMISASH
                     NPYPDNGIKFFSAEGFKLPDEVEDEIEALYFQETDDLPRPEAAAIGRVFQDITAGERY
                     LQYVLSTVSCRLDGYRVVLDCANGSAHNLAPQAFRALGAEVIACYDAPDGININHQCG
                     STHPEAVAAMVQMHQADFGFTFDGDADRVLAVDGHGQLLDGDVIMTILAQDLQEKGQL
                     KHNTVVATVMSNLGLEKAAQAHGFELVRTNVGDRYVLEAMLAGDFSFGGEQSGHIILL
                     QHNTTGDGLLTALLVAAVAAKKKQSFAELAAAYKRYPQVLVNCRVGRRDGWDKNPCIK
                     EAVAQTEAQLAGVGRLLIRPSGTEPLIRVMLEGTNEAELLQLADDLVTVIQDELD"
     gene            complement(46641..47573)
                     /locus_tag="VFC74_04320"
     CDS             complement(46641..47573)
                     /locus_tag="VFC74_04320"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HZK24593.1"
                     /translation="MMERILKNNIVLKIIAFLMALLFWAYVTGDNMYENLLTRTYSDV
                     PLAWLNLAEELELVEMPQEVQVVLSGPADVLNNITPLNLKAFVDLRDLAAGQHRLTPT
                     AEVPNKVKVLSFNPQQVVVELEEVESPHMPVVLDVTGAPASGFIRGEARINPSSVFVR
                     GGRTRLNNVDRVRVIVNIDGIARDWSQVVPVQAVDRAGQVVEGIEISPSMVEVFIPIS
                     EPQKEVSVKVATVGKPPAGYAVQQIKLDPTTVIIEGSAEYLTTVAELSTETVDLSNLT
                     ESTTLNLQLEIPQEVKLLFEGQIKAEIIITAEVD"
     gene            complement(47570..48385)
                     /gene="cdaA"
                     /locus_tag="VFC74_04325"
     CDS             complement(47570..48385)
                     /gene="cdaA"
                     /locus_tag="VFC74_04325"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HZK24594.1"
                     /translation="MTRILQLLSVFSWRSILDIVIVTFVFYKAIVLIRGTRAEQLVKG
                     LAVLVMITVVSRELGLAALDWLLTNLMTLWIVAIPIVFQPELRRALETLGRGKIFARS
                     NQIYADVDFELMLEEIIKAIQVLVKKRIGALIILERETGLKEIIETGITIDGHVSAEL
                     LINIFIPRSPLHDGALIIRGNRIAAAGCFLPLTENPNLSKELGTRHRAALGVSEQSDA
                     VSIIVSEETGVVSLTNNGKLTRYLDDKTLKQMLIDLCKPPSNSFNFWQWRSSQ"
BASE COUNT        13392 a        12066 c         9410 g        13613 t
ORIGIN      
        1 acgcaatata gctccgaaga tgttgtcgaa attatgtagc cggtattatg caatgtactt
       61 atgttaacaa gcaatttcgc cttgatacgc tgtttatgca aggttttgtt aataaaccct
      121 ggtatcaatt aacataataa tccacagtat ccacagagtt atccacagga ttaagcccat
      181 tattagcgga ttcttatcaa gtatccacat tatccacagc ttgttaccat aattatggac
      241 cctcaggaaa cttaacctct ttactgtact tgccataaat tgtctaatac tggggtagca
      301 tttaagtcga gtcctgcaaa tttctgtttt acatataagt tccagccggc agcagctacc
      361 atcgctgcgt tgtcagtaca taacaccgga ggcgggtaaa ttaagttgcg atttgcagca
      421 gctaaagcct gctccaactg ctgccttaag gaacggttgg ctgctacccc acctgccaac
      481 agcacagttt taacttgttt gacttccgcg gctaaacaag ttttttcaac taacacatca
      541 acaactgcct gttgaaaact agcagctaca tcagctgtct ggaagttttc acccttttgt
      601 tttaagttat gcaaggtgtt aatcacggca gactttaagc cagaaaaaga gaattcaaaa
      661 ttattatttt gcccggtaaa agcacgggga aaagcaaaag cgctgctgtt ccctgtctcg
      721 gctgctttat ctatggccgg gccgcctgga taaccgaggc ccagtactcg tgctacttta
      781 tcaaaagctt caccagcagc atcgtctcgg gtggcgccca ataactcagc ttgccagcgg
      841 gatgtgagat acaacaagtt agtatgcccc ccagaaacaa caagacagac agcggggaaa
      901 actacttcat ggacaagaaa atttgctgca atgtgggaga ggacatgatt gactgcaagt
      961 agtggcttat ctaaagcata tgccaaggtt ttagcagtat taactcctac cagcaaagca
     1021 ccgacaagtc cgggaccatg tgtcacagca atggccgcta gatctgcaaa agtacagttt
     1081 gcttccagca aggcttcctc gactacttca ttgatcatct ctaaatgatg gcgagaagca
     1141 atttccggca caaccccgcc aaatttctga tgcactggca cctgtgaagc aataatatta
     1201 ctcaaaatga ttttaccatc tgccactacg gctgcagctg tctcatcaca cgatgtttct
     1261 atcgccagaa tcaatttttg ggtcatcgtt tctgctctcc aacttgtttt attgcttgct
     1321 gttcgccaga cgtctctgct ttgtcagtgt taacaggtaa gcgttccagc cacatgatta
     1381 aggcatcttc atctggatag taattagggc gcacaccaca agctacaaaa ccatacttct
     1441 tatataatct ttgtgctatt tcgttcgaaa ctcgcacttc caaggtcatg cgttttgccc
     1501 cttttatggc agcttcggca ataagatgct ctaataattg gcgaccaagt ccctgaccct
     1561 ggaagcttgg tctgacggct aaagtagtaa tgtgtgcttc gtcaagtatc acccaaatac
     1621 cagcataccc caccacttct tcatccagca gcgccacata gtacgaagca aaaccattat
     1681 ccagtatttc attaataaag gcatgcactg accaaggtga ggtataaact tcattttcaa
     1741 ttgcgctgac tttatccaca tgaaaaaatg tcatcggctc aatagagaca ttcaacacga
     1801 atcgcctccc tcgatctggg tctgctgata cttaatttct gcttcagagc gccgaagata
     1861 agctggatgt aaatcacgcc aactttgcac ctgccctgcg ctaaactgag ccagaccaag
     1921 cctagcgaca ctagaggcac gacagatggc tgattccgga ggcataaagc aagcacgact
     1981 cactaaagct cggcttatta tctgccgata aacagggact gcatcaccaa caaataatac
     2041 aggctccggg cgctgctgca gctcttgcag tagttccgtt acagacaaag atcggtctgc
     2101 gaccagcctt tccacttgtg ttcccgctgt aaaaattgca ttatagacct gctcacgccg
     2161 agcatcaagt atcggacaga ttaatcccgg aaacaatgga tgctgcgctg ctaaagcagc
     2221 gagagtagaa atactacaaa gcggtaatgc caaggcctga ccaagtgctt tagctgttac
     2281 catcccgatg cgcagcccag taaatgaacc tggacctccc gctactgcta ccccggataa
     2341 atcttgtggc tttaaaccgg tatcacgcag tattgtatca atcaagggca acaaacgttc
     2401 cgaatgtgtt tttttgactt gctgtgtata ttcagcaagt gttttttctt ctgaaacgac
     2461 cgctacactg caaactaaag tggccgtatc gatgcccatc acatacataa ttgtcatctc
     2521 cccgccatta gggactctac aatttgccga tatggcttgc tagctgcttg caaaaccaac
     2581 tgacgatctg tcacgtcttg agagatggtt atagtcaaag caggccaact tgctaaatat
     2641 gcagcaaatt tattagccca ttccactaaa cttacaccgt cgccatcgag acattcatct
     2701 aaaccaattt caaagagttc actttcgtct tgtagacgat ataaatcaaa atgataaaaa
     2761 gggagacgcc cctggtgagc gtttaacaag gtaaaagtag gactggtgac gagcgtagtt
     2821 acgccgagcc cctgaccaat accacgggca aagacagttt tccccgcacc taaatcacca
     2881 tctaaatata taacagcgcc ggggaatagt aatctaccca cagctatacc cagtgcctct
     2941 gtttcccctt catgggcaga gtttataaca tactccaagg acttaaccac cttaaaaagc
     3001 ttgcgcttca atttcatctt gccaggtttt tatcctagct tcattagctg ccggcaagcg
     3061 atatcatttt ctagccgatt aaaaatgctg atagcctgta aactctagtt tttcccaatt
     3121 tccctctttt gtaatcatct gcaatgttct ttcttgtatc atctcgccca acgaatataa
     3181 gggcgcctga aaacgttcta gatacgcttt agctaaagcg gtataagcat cactttttac
     3241 cgtaaacagc agttcataat cttctccacc atttaatgct agcgtaattg gtgctatttg
     3301 gcgcaaaggg gcaatagctt ccaccgcagg atgaataggg actttctctt cccttaataa
     3361 aaccccacag ccactggctt cacaaatctc ctgtacatcc tttaacagtc catccgataa
     3421 atccagcgcc gccgtcacta ctccctgctg ggcaagccac aaaccttctg ccacccttgg
     3481 ccaaggacgg aaatgtgcct gcagggccaa ctggcgccat tctaatggac aatttatttc
     3541 tgcttgcagc aatagtaacc ctgccgccga agcacctaac aagcctgtag tacaaagaat
     3601 atcgccaggc tgtgcaccac tacgcaaaag tgctgcttct cgtttcactt caccctgtgc
     3661 agtaatactt atcactagtg gaccgggcga agccacggta tcaccaccgc tgacggccac
     3721 accaaaacgc tcggccaatg ctgtcaatcc ttgataaaag gccaggacaa actcgctttc
     3781 cagctgcgca ggcaaggcca gtgccacata gacatgacgc ggcaaactac ccatcgctgc
     3841 caaatcactt aaattgacag ctaaagcttt atagccgaga tcctctgctg aaattccggg
     3901 cagcaaaaag tggactcctt ctaccagtaa atccttactg gtcactagcc aagagccctg
     3961 acttacctgt gtgatggcag catcatcacc aatgcctacc atctccgaag ttacagtttt
     4021 ggcaactatc tgcgagatgt gctttataag cgctttctcg cctaattcac taattttcat
     4081 ctattaccac ctattccctg attatagcct taactcctac aaaaaacaag aaagacgccg
     4141 cgcatttttt caatgatgcg tctttcttgt tctttagggg ctgccgcccc ttcgacggac
     4201 ctgcgggtcc tgaccacccc gcaagattaa ggggattccc ccttaaaaac cccagcggat
     4261 aggaaaagta agcaattcaa ggttgatatc attcctatcc gtcaagaaag acgccgcgca
     4321 ttttttcaat gatgcgtcct gaccaccgca agctgttcat actatagttg cagttagttt
     4381 gatacgccgt caaaattagg gtatactgga aatgattcga ttattaaaat ggggtgatca
     4441 cgatgaaaaa atcccgcgaa attgttggtt taccagtggt tgatttactt gcagggagct
     4501 ctgtaggtcg cgtacacagc ctaattatcg atccgcaaac tcggcatgtg gcggcactgg
     4561 aagtgggaga acgtacactc ttaaaaacca acatcgagtt aatccccttc agtcaattgc
     4621 gcaatattgg taatgatgcc atcactgtaa tcagcacaga aactagcagc cagtcagaca
     4681 aagcagcaac actagagaac ctggcagaaa atcaattgtc tggcacccgc gtagtcactg
     4741 tagatggtac atttgctggt actgtagaag acttttcctt tagtccaact gatgggaaac
     4801 tggctgaact atttattatc caagaaaaac cacgcagcca tcttgcttgc cccgtcacag
     4861 tagttgaaac atttggtcgt gactttatcg ttgtcaatga aaattatcag acggcggtgc
     4921 gtaaaattat ccccccccat cctaacaata caccacagca tcttgtgcaa acgctggaga
     4981 gtaaagcgat agactttgcc ctcggtcggg aagtgagaca agaagtacta ggcgaaaatg
     5041 gggacctcat tatacgccaa ggggaagctg ttactgccga aaatattgct ttagcccgtg
     5101 aaaaaaatcg tttgccacag ctcctttttg ccgcgggcgt tggcgaatta ttggatggcc
     5161 tagattttac ccgtgagaaa ttagatgcag gcagtaaaaa aataatggat gcctggcaaa
     5221 acttacgagg ccgctcccaa gtctggttat cccgtaaact agatgatgat ctcggcagca
     5281 caactacaga actacgtgac ctttggcagc aagtacaagg taaacttagc caaagcggcc
     5341 gtgagctaga ggacgccacc cgcaaacaag ttcgttccta tgtcctaggc aaaacattag
     5401 ctaatcctgt atatgacccg gaaggtactc tactgggcgg gcgtggtgat ctagtcacag
     5461 aagagatgcg cagcaaagct gaaacagcag gacgactgcc gcagctattt ctctctgcaa
     5521 ctactggtga tgtccaaaac gtcctcgaac cgatcaaaaa acaaatacgt aatatgcttc
     5581 gtgaataaac gaaatgtaaa gtccaaagta acaaatactt tggactttac tgtttttatc
     5641 tcattgatgc ctttgcttcg gccacacagt tatcagcaaa cttaaaacag cgcagcctgc
     5701 tctggcacta acaagttttc ttactgccat ttttctaaaa gaccattttt aattttctgt
     5761 ctggcagcta aagtcgacac tgacaagcca gcgagttgat caaatagctt tattttaaac
     5821 atggccggac catccgcctc cgccgcggct ttttctgccg cgacattgat tgctgccaaa
     5881 ccagcagccg cagccacaag atagtttctc tccactgccg caaagcaacc tattaccgag
     5941 gctgtcatac aaccggtgcc gacaactttg gccattagtg gatgaccgtt atgtacttca
     6001 agtaactgct gaccgtctgt tactaaatct accgcaccgg ttacgaccac taccgcacct
     6061 gtctccctgg ccaatgctgc cgctaacggc actacattac tcgggacagc gatggattcc
     6121 acacctttaa ttttagccgc accgccagct aaaagagcaa tttctgcagc attaccttta
     6181 atcacactca attgtaatgc tgctagcaat tttttggcac ttgccgagcg taaccgcgta
     6241 gcccccacgc ccacggggtc taggataatt ggtcgctgta gctcattggc gcgccggcct
     6301 gctaataaca tggcctcgat ttgagctggt gtcagtgtac caaggttaag gactaaggcg
     6361 tctgcactgg ccaccatctc tgccacttct tccacagcat gtgccataat cggcaaacca
     6421 ccagcagcta aggtaatatt agcacactca ctaatggtga cttgattagt gatatgatga
     6481 atgagcggct ggcgttggcg taaagcagta aatattttac ctgtgtccat ctatttccct
     6541 ccttataaaa aagcttgcgc tttcagtttt catttgcaag cttttatcct ggctgcaaat
     6601 gagtttcgct agttgcgata tcagataatt tccttgccgt attaaagaag ccacggaccg
     6661 taaatgtccg tggctttgtt ctgcctcgca gcggtccttc cctacgctgg aataatccat
     6721 ctcaggttcg aagggttaag tggcttacgc gtgccactct ctcagcccat actaacgggc
     6781 acccctaggt tactatgaag ttatgctttc tattttatcc aaggacgcct tcttctgtca
     6841 agaccgcttc taaagtgcta attaaacgtt tattctgctc agctgtaccg atagtgatgc
     6901 gcagaacatc aggcgcaccg aagatatcgc ctgtacgcac aataaccccg cgccgcagca
     6961 ttttattaaa aaccttttgg cagtctgcct tgatttctac ccagatgaaa ttggcctctg
     7021 tgggttcata aagcagccct aaacggctaa attcgctgta aaggtaagct tttccctctt
     7081 catttacttg ccgactatct gccaagtgtt ctttatctcc cagtgccgcc agtgctgccg
     7141 cttgagaaag cagatttaca ttaaaaggtt ccttgacgcg gcttaacatg ccgatgagct
     7201 ctggtgtgga gacaccatat cccacgcgca atcctgccaa accataaatt ttggaaaagg
     7261 tgcgcagcac aataacatta cgtcctgccc gcacataatc tagcgtttgc ggatactgct
     7321 catccctgac atactcataa taagcttcat cgaaaacgat aatcacatct tccggcaccc
     7381 gtgccataaa agcctgcact gcatctttct ttacaatggt accggtagga ttattgggat
     7441 tgcagataaa aacaagtttt gtcttttcgt taatggcctc agccatagct gtcaagtcat
     7501 gagttagatc tttggtaggc accaccaccg ttcgtccgcc catcaccttt acagcaaagt
     7561 catactcgga aaaagtagga tcagcaacaa tagcctcttc cccttcatct aaaaaagctt
     7621 ccgctatcat tttaataatt tcatctgaac cattgccgac aattaagtta gccggtaaga
     7681 tgccaagctt ttccgctaaa gctgttttta aataatagca attaccatcg gggtaaaccg
     7741 aaacgttatc tagcatcccc tgcaggacac gaatcgctgc cggggcagga cctagagggt
     7801 tttcattcga ggctaactta attacatcgc taatacccag ttcacgttcc acttcttcta
     7861 ccggcttacc gggcacatat ggtttaatag ccagaatttt ttcccggtgc agttgttttc
     7921 tcatcgactg acctcctaaa tttggcgttt atcaatgacc cgcttggctt taccttcgct
     7981 gcgcgggatt gacttcggtt ctactaaacg aattttagcg ctaataccta agacagaatc
     8041 aattttacta tgaatttttt gttccagttc ttctaatctt tttattttat ctgagaacat
     8101 gctttcagat acttccactt ggatctccaa gagatccaaa ctaccttgac gcgttaccac
     8161 taactgataa tgaggttctg tttcacctac ttccagcagc acactctcta cttgagtagg
     8221 aaagatatta acacctcgca caataatcat atcatcacta cggccagtga ttttacccat
     8281 gcgcacatgg gtgcgtccac acaaacaggg ttccagtgtc agcgtcgtta agtcccgtgt
     8341 gcgataacga attaccggca gtgcttcttt ggtaatcgtc gtcagcacca gctctccttc
     8401 ttcaccgtaa gggagtactt caccgctagc cgggttaata atttctggaa taaaatgatc
     8461 ctcgaaaatg tgcagaccat gcttttcatc gcattccata gctactccgg ggccaataat
     8521 ttcacttaag ccatagatat ccatggctgt aatcccaaga cggctttcga tttgctgccg
     8581 catcgcttca gaccaaggct cggcaccaaa gagtcctgct tttaactgca aatcgtcggg
     8641 caccaaaccc atttcagaca ttacttcggc cagatataaa gcataactag gtgtacaggt
     8701 caaaatgctg gtgccgaaat ctttcatcag cataatctgc cgctgtgtat tgccgccgga
     8761 agcgggcacc acggctgccc ctactaactc tgccccgtag tgtacaccta ggccaccggt
     8821 aaatagtcca taaccataag cattctggac tacagaatta cgagttgtgc cggcactggt
     8881 taaagcacgt gccattagtt ctgcccacgt agtaatatca tgacgcgtgt aacccacaac
     8941 aatgggttta cccgtggtgc ccgatgatga atgatagcga acaatttcat tttgtggtac
     9001 cgcaaataag ccaaagggat aagtatccct aaaatcttgc ttcactgtaa agggcagctc
     9061 agacaaatcc gccagttcat taatgtcacc tggttctagt cccgctgtct gaaatacttg
     9121 agcataatgc ggtacgctta atacccgctc tagcgtcttc ttcaggcgct ttagctgtag
     9181 ttgctgcaac ctgtcccttg acatgcactc agcttcttga ttccaataca aggtctaacc
     9241 cccttctatc tttgacgctg tcttaacaaa gttctcctgc ctaatgccaa gaaaagcgac
     9301 cttcttacat gcataagtat caaaaaaaag aatacccata taacagagat tttcacaaaa
     9361 cttaatataa aacagcataa aataaattac ctttataatc taaggaggct aagatggcat
     9421 gatagttagt tttgtgctat cacgaaaaag aaaacgcata atcttactgg tgatagctgc
     9481 ccttgtacta ctatgtttag taaaaatatt ctgggcaaac cagaccagtg ctactgttgg
     9541 ccgaaaacgt cttgttccca tctattatgt ggacactact gaacaaaaaa tcgcgatctc
     9601 ctttgatgcg tcctggggag ccgaatttac gccgaaaatt ttagagatct tgcgtgaaaa
     9661 caaactaaaa acaacctttt ttttaaccgg tttctggatt gacaagtacc cagaactagt
     9721 taagcaaatt gctgatgaag gccatgaatt aggcaaccat acggcgaccc acccccactt
     9781 aaattccttg gataaagccg ccatcaccaa agaattaact gctgttcaca actcactgca
     9841 agaattatcc gggcagaaag caatcctctt tcgccccccc tttggtgagt acagcgacaa
     9901 agtcattgaa gcttgtgatg actttggcta caagacaatt cagtggagtg ttgattccct
     9961 cgactggaag gaaatcagca aagaggatat cgtagcgcgg gttaccagcc aattacatcc
    10021 cggtgccatt gtgcttttcc ataataacgg ccgctacact gccgaggccc tgccggagat
    10081 tatcagctac gcccaagaaa atggttatga aattgtgcag atttctcaac tcctctatca
    10141 agatgattac tacattgatg ccaatgacgg tgctcaacgt aaaaattaac gtttcgctgc
    10201 aatcaatccc gcgcctatta ataaggcgat tatttttttg ctcttgcctg tcaaaaatag
    10261 ctgttagttc ttttttttcc aaaaagaatg attaataaca aaaggatccc caagctgatg
    10321 ggagtaatat agccaaaagt acgattgacc ctaccggcaa ctttatctgc cagtgtcact
    10381 ttttctgccg caaatagcgt aaaggttaaa tcttgcccgg gtaagccggt aaaatctgcg
    10441 ctgtactggc gatctgctgt ctgttttaag ggcagactgc tgttgataag ataaggagcc
    10501 tccggggagg taataatttt aatctgcaag tctttaaact cgctccagtg tgcggccgga
    10561 ttaagcagat acgtaaaaga atactgcggg ctaacagttt cccgtttatc catggtgcct
    10621 gaagttttgt aactaacgct tatttcttgt gcccgctgcg gtggaaactc taaactgtaa
    10681 agtaacacca tgatacgctc ttgttccgcc agcgcccata gctcttctaa cgcacaaacc
    10741 cccaaattct gggtaaattg ctggtcaagc tcttctgcat ataaattgta tacttgagta
    10801 tcgtctagta atgcagctgc ctctgtatta agatggtgct ggcttaagga cactaaatat
    10861 agcttaacct gctgctcctg cgtggtaact tgcgccgtgt acaaatctgt tttctctgaa
    10921 agctcgccat ttgtgtaggc ctgcatggta aattcaagct cctcgcccaa ggcaaagact
    10981 tcaaactgct gcggctcact acaccaggct gacagccgca cctttcctgc ctcacgtgca
    11041 aaaccattaa aaccatttat caggatctta gtattttcag cattatcatt aaaggtaact
    11101 acaaaattta tgttttgctc cgttgtgggg ctaacgtcaa agcgatacag ccggcctatc
    11161 tcctcagccg agaaatgtgc cgcactatat tgctgccggc taagagcaga caaaatattt
    11221 gtaaatgcaa aactaggaac tacgtctgtt gcagaaaccc tactgttgaa ttccgctcgc
    11281 gaacctaggt agagctcgta aggcagctgc tgggcgtccg ctttgatcac aatctcagct
    11341 agattcaatt gccgcagctg cccaacaaaa gggaaggcca tttgtactgc ctgctgttca
    11401 ctagtaggat tagacatctg ataagttgcc gtaactttgc cctctaaagc ataagaagaa
    11461 gtatgcttct gggaaaaatc aaaaaccagt tcttcacttt ccacagtaag cggcgtatct
    11521 gttgcaattg acatcatttc agctgcaggg tagccctgcc aatagacggg ccctgaattt
    11581 gcaaagacgg ctgtacacaa agtaaagatt agcaatacag ttgcaatact cataacatac
    11641 ttggtcatcg cttcacttcc ttacttcacc ctgtttattt actaagctgc agcagcaaaa
    11701 gattactcta aagcttggct gcgtaagacc agatgaatat gatcttccca gctgccgtta
    11761 atctttaaat atttttttgc taacccctca tagtaaaaac ctaacttctc tactacccac
    11821 agtgagcgct tattaccagg cataatatta gcttcgattc tatgcagtcg cagttccgta
    11881 aaagcatagt ttatgacttc tgccagggct tctgtgagca aaccttggtt aatttgcgcc
    11941 tctgacagtt tatagcctaa gtgacaggat tgaaaagcac cccgcactat attgtttaaa
    12001 gcaatctagc ctataatttc ctgcccctca ttgaaaaaca accagacctt aaacagccgg
    12061 ccctctcgca tggagtgcag ttcattttgc agctgatcct gttgataagt cagggtaaaa
    12121 aactcctctt ccttaacagc ttcccacggg gccagaaact cttgattacg ttgataataa
    12181 gccaacactt gttcagctgc aactaagacc cgcaaatcta aacgagaagt tgccaaatta
    12241 atatccttaa caaccaatag tgggccctta aattgcatgc ttaatcatcc ccagatctac
    12301 ttatctgttt aatgtcttgt agtgcatact tctgattaaa actaaaatcc cctgctcttg
    12361 gtggtagaat tttatgatca taaggcccgg tcattgacaa attactcacc acttgcctga
    12421 ccaaaaaact gcagtaatgc ctcggccgtt tggcctcact ttgatatcca agatagtaca
    12481 cacctcacac gtcatcatta aacggtgacc atgactaatg gcgtctttat ataaaataaa
    12541 atcgcccctg ttatcaataa caggggcgat taattattcg cggtaccacc ttgtttttgt
    12601 tgttgaaaaa caacaacctc tgcgggcagc tagcattgcc cctccacatt aacgggtgga
    12661 atccggcaca gcctacaggg catttgcctt tcagtgtgcg gctcaaagga tgaattcaca
    12721 gcgctaatac ttgctctctc acaccaaccg aaagctctct gcattgatta gttaagctgt
    12781 tatgattcct cgtcatgacc tttaaatatg tgttgaaatt tatattacgc ttatcttatg
    12841 ctaaagattt ttatttgtca accacttttt tcatagccat gattacttag cggttacgtt
    12901 acattttagc ttaacttgtc acggttacgc aactgttcag ctttctcttt gatggtttgg
    12961 acaaaatctt ggattctctc ctgccaagag gcagtatatt gatcaccatt gcgcaacaag
    13021 tccatggttg tatcactaat taatatagcg gtatgtctaa ttttctcttg tactgacgct
    13081 aaaaagatat cacctttttc agtgtaagga agtgctttgc taaaccatgg tgccagtttt
    13141 tcacgaatag cgcccaaagg agtgttcagg aaataaaagc ctgccgcaag cactgcactg
    13201 acagcgaaca tccaaaaggc atttctggct gctgcacgac ctacattgcc tgctccacca
    13261 ccccctttac tggcgggact ttgcagcggg gcaggtgctg cccgctcatt gttatcatca
    13321 tcctcatctt ccgggccata gaaataatag gaaaccacat cagcaaacag ggcagcgcca
    13381 tcttgtgctt tctcacgttg attaagatgc gttcctacct cgatcaacat actagttgct
    13441 gttaaatcct gattatagtt accgcgggcc ataaagatgc ccttaactag gttggggtgt
    13501 atttcatcgg cagtgttttt taaatcaagg gcaaattgac ggacggtctg catctgggga
    13561 ttttgcctgc cgacaacaaa ttgaacttgt gtaactgctt cacccgccac ttcagctgca
    13621 taaacgttag caggaccagc atcacggtgc acatcaaaaa taacatccgg cccctttttt
    13681 aagatctctt cagctgttac ccgcgaacgg cggtaagcac cacggtcgtg cggtaaatgc
    13741 agtgtgttgc tgcggatcac cttaatatct ttttcttgta aggctttagt gaaggaatcg
    13801 cccactttat gaatgccgcc cttacccaca atactatccg taccgtcagt cggcacatat
    13861 gattcagcat tatgggtgtg atagacacca attaaacgtt tcggttcagc ctgtggttgt
    13921 aaatcctcat tttttttatc tttctgatct tttttatttt tagtcttctc cttttcttgc
    13981 ttatcctttt ctgtcttact tttttcttgt ttctgtacag gttgaactgc tccgaaaaaa
    14041 cgctgctgta accttactaa taagctttgc ggttcctcgg caaagatagc accaacctcc
    14101 cgtataaaac gagcttcagc cacataatta cgtactcggt aaacccgata tagttgatta
    14161 ttcgaagtaa gatattcatc accgatatga atttttcggc ctgttaccat gatctccttg
    14221 ccatcttcag caaccatgcg gtagtagttt tcagctagca agtcttcgcg ggaaatctca
    14281 cctgtatcct gattatcaaa gaaatcaaaa agagagcgag aagtggttgt tagttcatta
    14341 cttgtgagca taaaaaatga gcataaggtc acgacaaata gaagaacaag aatttttctt
    14401 ttcatacccg gtggcctcct tgcaatcttt ccaagatttc gcccaccaac tctgctaata
    14461 atacagcgcc catgccagca ataaccgctg ccccatagac ccctgttgag cctaaaacga
    14521 tgggtacatg gagttggtaa agaaataagg tctcaagcca agcaaataga tcggtgagaa
    14581 taacaccaaa gataccgcca ataaaagcgc tgcgtctgga ccgtcccagt aagtaggccg
    14641 tgacagctgc gacaattgcc ggcataaata atggatctac taaaaaagca gctgaagtag
    14701 gttccattgg cagcaacttt tctgttaaat ataccagtac ggaaactact ccggctgtaa
    14761 ttagggctct gcgtttttct gcagcttcat tagcagtaac aattaaatag accgctacac
    14821 caacaggaat caaggcgccg ccgatattaa gggaaagccc agcataaata ggtatatttg
    14881 gcaaaaaacc agcagccagc atcgccagta aaattactaa ggcttggatc tttgttaagc
    14941 gcatgcgatc tagaacgcga tgcaaaaaac ctaaatatat tagaactgta acaacaatta
    15001 ggcttaaagt gcctacgttg gtcgtaaaca tggcctcaca ccttccttct tactctcgtc
    15061 tgaagctgct ttagttttcc ccacaaaagc aaaaaaaaca cacctcaaag gatgtgttca
    15121 catattataa tgcattaaac actgttactc acagcttccc tgcagtatca gcttagtcaa
    15181 acgggttagc aagtgaattt tgctttggga gagataaaaa ctatgttacg tagtattgtc
    15241 aacaaaatga aaagcgcagc tcacttctta aactacccta ttttacaata cattcccatt
    15301 tcgatgcata gcctgctgca aaaagacaac tggcataatc cctttagcca agcaaccccg
    15361 caactgctgc tttttcaaaa caaagctgct tttgaaagtg cgctgcagca ttatcaagtg
    15421 cagctctcag ccccgatacc agcgcaggag ctgtatgtaa tctgcctaaa ctttaaaagc
    15481 ggtttagttc tctttcgtta tttaacctgc aagtttgtgg gcacgccaca gctaggcggt
    15541 attcatatct ttgctgtcac caaaaaatat tttcccccgc gtgccattca ctttgcgctt
    15601 tatacggaag acggtagaaa attacgcagt atgacacaaa aaattatcta atggtgatct
    15661 ttatcctcat tcgttaagct cattttctac cttgctgttc atgacctgct gcctgtgaga
    15721 aagctgcaaa gatagcagca gcatgcccat acttgtgaga taaccattcc gggtgccatt
    15781 gcacgcccag tacaaatcga tgggacggca gctctactgc ttcgataatt ccatcatcag
    15841 ccacagcaca tactttcatc cctgcgccaa gcttacgaac ggcctggtga tgcagactat
    15901 tgacacgaaa actttgttta cctgtaattt ggtaaagtaa tgaattcttg cttacgctga
    15961 ctcgatggat tgggtaggaa cgaggagcct gctgctgatg ctgtaccttt tccctagcag
    16021 caagatcctg atacagctta cctccggcag ctatagctaa aatttgtgcg ccacgacaaa
    16081 tgcctaacag gggcagacct ttttttaagg cagcgcgtgt caaataaagt tccatgcggt
    16141 ctcgctccgg ctgcactaaa ccttgctgcg gcgctgcttg ctcaccgaaa tgataggcgt
    16201 ccggatcacc accgccacta agtaataaac catcaactgt gctcaacatc tgttgatagc
    16261 tcccaacttg agagcaaaca ggcaacaata atggcacagc ccctgtggca gcaactgctc
    16321 gcacatacat ctgagaaagc ctactttcac ctcctacact attgcaagta ataccaatac
    16381 gaatggtcag tgggacaacc ccctaaaaag cgaaatacgt aaccgtggtt acgtatagtt
    16441 cacttgactt aagatatttt aaaaatctat taatcccagg gagatcttta gagcttggtt
    16501 gactttttgc attactcgtt catctaattc tgtcacttta tcctgcaggc gttgtttatc
    16561 aatagtacgg atttgttcaa gtaagatcac agaatctttc tccagtccat actcttttgc
    16621 tgcggcttcc acatgtgtgg gcagctttgc cttatcaatt tgtgaagtaa ttgcggcaat
    16681 gatgacagta ggactatatt tattacctac gtcattttgt attaccagca ccgggcgtac
    16741 cccaccctgt tcagaaccga tcacagggct taagtccgca aaaaagatat acccccgttt
    16801 tacttccact ctattcactt tttacccacc aacaattctg cgatcaaatt ggcttcattt
    16861 tccacatcaa acgcttcatt ggctaagcgt aaattcagtt ttgccatctc agcgtagcct
    16921 ttttgcatct tttcgcgaat ttgctgtttc tcccgctccg caagatatag gcgcatcgct
    16981 tctcgaataa aatcggagcg tgttcttttt tctgttgcaa cgatgccatc tacctcttcc
    17041 aacaagttat acggtaggct aatcataatt ctttttgttt cagccaaata gagcaccccc
    17101 taaatttcat cttcccccaa gtaaatcctt ggtactcgac gacttattgc agtgacaact
    17161 tcatagttaa tggtatctaa ccaagaagca acttcatctg caggtaactg ggcgccattc
    17221 tgtcgcccaa atacgacaac ctcatcgcct gcttgcaccc ctgccacgtc ctgcacatta
    17281 atcatggtct gatccataca gatacgaccc acaatgggaa cacgttttcc atgcactaag
    17341 acttgaccaa gattagagag ctgacgtcgg taaccatcac cgtaaccaat attcagtgta
    17401 gcaatgcgtg ttggttgtgt tgttacgaag gtgctgccat aagaaacacc aacaccggct
    17461 ggaacatctt tgatataaat tattttagct ttcaaagtca ttgctggtcg caactggatc
    17521 tgcttctggc ttacttctgc agaaggatac aaaccataaa gaccgatacc taaacgaaca
    17581 atgtcaagct gtgcttccgg catacagatg gccgctgcgc tgttagccac atgcttaaaa
    17641 ggaatcataa ttccttgtgc ttctaaacgt tgaaccagag cgagataacg agacagctgg
    17701 aggcgggcaa aagtcaaatc tgcctggtct gcagaggcaa aatgagtata aaggcctgcc
    17761 acttgcagtc ccggtaaggc agcaatctgc tggacaaaac tatctgcctc cccagcttga
    17821 cagccgacac gcgccatccc cgtatcaact ttgatgtgga catcaagtct agttgccgac
    17881 tgcacagccg cccgggaaaa agcttgtgct tctgccaagg tgaacacaga tggcgttaaa
    17941 ttgtattgta ccagataaga agcatattct tgcggtgtaa accccagaat caagatcggg
    18001 gtcgtaagac cattttcacg cagctcaata gcttcttcta ccatgccgac acataaaaat
    18061 gaaacaccgg cggccacagc cgttcgggcc acctgaacag ccccgtggcc gtacccgtcg
    18121 gcttttacaa tcgccattag ctgggtccgg gatgaaatat gcaagcgaaa ttgttgtata
    18181 ttatgtctaa tcgcaccaag gtcgatttcg gcccaggtcg gtcgtaatgg gagcccgcac
    18241 aaaacagagc accacctatc tttacagtta atattgtatt ccacagcaga cttgattatt
    18301 cctgccggta atgattggct agttgttcat ccctatggac attcacatct ttcttgcaag
    18361 ttaaaataac tatggattgc ttgcgcagct tcatgtaagc gatcagcggc aatcaagcag
    18421 gatattttaa tttctgaggt ggaaataatc tcaatattga taccagcttc cgctaaagct
    18481 tggaacattc cggcagccac accagtgtga tgaatcatac cagcaccaac tacagagatt
    18541 tttgccacat tccgttgata acgtagtgct cttgccccaa cttcctccac caacggctgc
    18601 agaatagtgt gtgcttgctc aaaatcatct cctgccaacg taaaaagaat atcgttttct
    18661 ccttctcggg ggatactttg cacaattaaa tcaacattaa gcccagcttg tgccacagca
    18721 ttaaagatgc gggcggcaat tcctggacga tccggcaccc ccatcaccgc aaacttaact
    18781 tgattttcat cacaggcaat accgttgatc acagttccct tctccaaacc tatgtccccc
    18841 ttaactaaag tgcctacctg gtcatgaaaa ctagagcgga catgaatcac cacatggtgt
    18901 tctttgccac attccactgc ccgcggatgc agaacttttg cccctaaact tgccaattct
    18961 aacatttcat cataagtaat cgccgttagt ttacgggccg tcggtacgag gcgagggtca
    19021 attgtataaa ttccatccac atcagtatat atctcacagc tctcagcacc aatagccgca
    19081 gctaaagcca ccgctgttgt atcggaaccg ccgcgcccca aggtatttac ttccccggag
    19141 gcatcaatac cctggaagcc agccacaact gcaacttcgc cagccgccaa agccttgcgc
    19201 acagcgtccg ccttaaaatc acaaatacgg gcgttcagag gatttccctt tgttaagact
    19261 cccgcttcgg caccggttaa ggatactgct gcctggccta aagaatgtaa agccatcgtt
    19321 agcagcgtac tgctgacctg ttcacccgtg accagcagcc tggctaattc acgttcgcta
    19381 ggctgtttag agatttgttt ggccagtgct gttaaatcat ccgttgtatc tcccatcgca
    19441 gacacaacca cggcgacacc atgccctgcg gctaccgtgc ggactatttt tcgtgccaca
    19501 tttagtattt tttctgcact accgactgag gttcctccat atttttgtac tatccacacc
    19561 atattttatg cctccgtcaa ctctgcttat tattctacaa cacgtaatac gctgttgatg
    19621 ctgcggatgg aagccatcgc ctggatacta tttaaagctt gtttaaagtt accttccctt
    19681 acatcatggg ttaccaagac gatttctgca gttccttcta cactgcgctt ttggataatg
    19741 cgatctaaac taacagcagc atcaccaaat gcggtagcca gtttggcaaa aacacccggc
    19801 tcgtcttctg cctttaagcg taagtagaag cgtgaatact gctctgccat cggcacaata
    19861 tgtttttctg taaaggtcgc ttccacaatg ccgttttgaa tctcattttt gagcgcccgt
    19921 gccgcatcca tcacatcagc acagacagcg ctgcctgttg gcagtgatcc ggcaccccga
    19981 ccatagaaca tcacatctcc cacagcatca ccttctagga aaagagcatt aaattcgttt
    20041 ttaactgccg ctaacgggtg ctctaagggt attaaagtag ggtgcacgtg cagttttaaa
    20101 ccctctgtta cttcttcacc aatagcaagt aacttaaccg catagcccag ctcttgtgcg
    20161 taatgaatat cacgcaattt aactttgcta ataccttcga caaagacatc ttcaatatta
    20221 atgcggcttg aaaaggataa agaggccaga atgctcagct tataggaagc atcacggcct
    20281 tctatgtctg aggaaggatc ggcttctgca aaaccttgcg cttgcgcttc tgccagcgct
    20341 tcagcaaatt ctttgcctga agcagacatt tgcgttagga tatagtttgt tgtcccgttg
    20401 ataatgccca taaacttctt gatgcgatta gcggctaaag aatattttaa cggacggaca
    20461 aggggaatac caccaccgac acttgcttca taataaacgt tttttccttg ccttttggca
    20521 atagctagta gttccacacc atggtgagcc attaaatctt tattagctgt gaccacaaat
    20581 ttgccatttt ccaaagcacg agtaatcgct tgacgcgcac cttcaatacc gcccatagtt
    20641 tcgaccacaa tatcaatctc tgggtcaagt aaaacatgat caatattatc tgtaaatagt
    20701 gcacggtcta aattgagtgc ccgtggcttc tgtacatcac gtacagcaat tctcttaatt
    20761 tctaaattaa cccctaactt gtgggcaatg ttattactat ttcccgttaa tatttccacc
    20821 acaccggtgc ccactgtgcc cattcctaat acgccgattt taattgtttc tttgctcatc
    20881 ttctccactc cattcttttt gctaactttg tccgactagt tccagttttt gcacaccggg
    20941 aattgctgcc agccgtgtca ataatagatt gacatcttca cttaaaccat ctgtttcaaa
    21001 agaaattgac gcattggcaa cgccttgcag gggtatgcct tgattaatgg ttaacacatt
    21061 acctcctgct gtggctattg cttttaaaac atctgatagt acgcctgccg tatgctctag
    21121 aattaatgaa atggttaaga ttttaccgcg gctagcttcc ctaaagggaa aaatattgtc
    21181 gcggtactta tagaaagctc cacggctaat accaacgagc tgcacagctt cattaattgt
    21241 ttttgcttta ccatttttta agagctcctt ggcctcagcc gttttgcgta aaacctgcgg
    21301 caagacatgc tcttgcacca aatatagcct atcatcagag ttttgcatta cgccctcctg
    21361 ttcactgcat gtggacattg atccatgcat gatatacatt atagcaccgc cttccctctg
    21421 ctgcaatact ttttttatgc agcttccgct caggcctttc tcaagcgtat accggtaact
    21481 acctaccgct ttgctagtta tcatccgata ttttatgagc aaaacgaaaa aacccgcctc
    21541 gaggcgggtt tccctatgga gtaggtaaac agataaaagt gccgatctga agatttttcg
    21601 ggttgatacc ggggtttagt gctaggatgc cttcgacagt aacaccttgc tcttgggcga
    21661 ttttaaatag agtatcacca gggacaattt ctaaaaataa acccgagggg caaaccggca
    21721 gcttctcagg attcggggca ggcccaggtc cccccggaaa ttcatccaat cccatgcttc
    21781 cacctcccct aacatcctat tcagctgccg cttgagatgt gttctaaaca ccttctggtt
    21841 taagtgattc agcgatctcg atcatttctg ataatggtag ggcaccagtc aaaacaaaat
    21901 cgctctgctc attggaccac cacagcgtac cgagactacg atcactattt tgttgataaa
    21961 ggccggtacg ttggccaact tgcactttac tagtctttgc actgcaataa atattctcat
    22021 ctgccctggc ctgctgtatt agtgtaaaat tcttgtggcc ttgataaaca agaattaaat
    22081 gttcgcgatt ttcttccatc gtccgggtga caacaaagag agaacttcct gttggtaaat
    22141 atgtaggtac aaatattgaa ccaggccacc cctcccgtgc ttcagccaga gttaaaggtt
    22201 cccgctggca ctgtgtagtc acttcacttt cagcttctcc ggaaaacttg aataattcag
    22261 ctggcagagg tggatttagt tcaagttttt taacagtaat gcggtttaag agttgttcat
    22321 ccaaatatgt ctcgatcaac atgggaaaaa atgttttttt gtccagccat agctgcaggg
    22381 tttcgcctgg cagttgacct gcacagctaa gattatacat cttttcaccc tgataatctt
    22441 gttccccctt aaaggtaaaa gaatcagctt gctgcagccg ttgcaaatac ccggttagca
    22501 taaagggggg ggctagttct aacgcgcctc gttcagcatc aatgcggtag taatcaccta
    22561 accctggctg gtagacccaa atctgctgac catcacagat aaaatgttga gcttctgccg
    22621 ctgcctttac ctccactcgc caccggttgt ctgccgtctg ccactgcttt acttggtagg
    22681 attgctcacc ccccaagcta aaaaccaaca cctccaactc agcataaaaa ccatctagct
    22741 tctccactct tttttctatc tgcgtaagta atttctcact ggtaacagca cagccactta
    22801 agagtaaggt tagcactagt aagcccgcta aagacctttt aatcaatccg ctcgcccctt
    22861 taccccaaag attatcatta gctcagcaaa aacactactg ggaacagttc cgacccttct
    22921 tgtttaaaac atggcactca acagatcctg acgactaaca atcccctgca agcgcccatc
    22981 agttcccaca acaggcaccc ggttaattcc ctgctccacc atcatcgtgg caacttcttc
    23041 tttagaggca tctgctgcta cggtaaaaac gggagtagtc atcacatctg ctactcggta
    23101 accagacatt ttctgtaatt gttttttaaa attgtctgga tcctcgagga aaattactcc
    23161 tcctaatatt tccgtaaaag caggagtatg cagttctttc gcttgataaa tcaaatctgc
    23221 ttctgtaacc attcctacca cctgctgttg ttcatcaaca actggtaagc cactgatttt
    23281 gtgctccagc aataatcgcg cggcttcgtc cacagttgtt tcttgcgtca cagcaattac
    23341 ttcgcttgtc ataatttcct tgatgctcat cgctgcactc ctcttttata tatctatttt
    23401 gctaatttcc agatatgcct ggggaagata gtcaagcaaa tctcgagcca caagtgaagc
    23461 cttaccaaga gcatgtgctg ccaaatcacc agctaaacca tgccaataaa ctgctagccg
    23521 tgctgcttgt gctgctggga gaccctgcgc cactaaagag accagcagac cagtcaacac
    23581 atcaccactg ccagcagtag ccataccatt attgccacta ctgttgatcg ctgctgcccc
    23641 atcagggtct gctactatag tatgcgcacc ttttaagaca acagtactcc gaaaatgttt
    23701 tgctaaatat tgtgctgccg ccaaacggtc tgtttgcacc tcttgtgtag tttgccctaa
    23761 caaacgggca gcttcgccgg gatgtggtgt taggatcgtg ggagcctgac gttcgcgcaa
    23821 aagttcaggg gctagagtca acaaattcaa ggcatcagca tcgagcacta ctggtaaatt
    23881 gcattccttc agtaaccccg ctagcaaggg cagtaattgc tcacgctgtg tcaagcctgg
    23941 tccgatggcc acagcatggc actgcggcca cttttcctgc agcatagtta atgctgtcgc
    24001 ctgcagctga cccgtctgat caacgggaag cgatacggta attgcctcca ctgcttgcgc
    24061 agccaccatg gagtgaatag agcggggtat agctaactgc accagaccgc agccgccacg
    24121 cagcgcagca gcagcggaga gcaccgcagc cccagaaaag cctggtgaac ctgccactag
    24181 cagaactgta ccaaaagtac ctttgtggct gtttgcttct ctttccggca gatgtactgt
    24241 tgtgcttgtt aataaagtgt ggctgtaacc gcttaacagg gcaggcggaa tcccgatgtt
    24301 taccacctgc aaacgccctc ttttagctgc tcccggatat aagaagtgcc ctaactttgg
    24361 caaggcaaaa gtcacagtaa cgtctgctcg taccgcggca cctaaatcat gaccggtatc
    24421 ggcattaata cctgaaggca gatcaactgc catcaccgcc gacgcacact cgttaagctg
    24481 ctgaattagc tgttgataaa actcatcaag gggacgagac agcccagtgc caaaaatagc
    24541 gtcaacgact aaatttgctc ttggcagcgc tagagaaaaa tctcgccaca ccgttttgtc
    24601 acgtaaacaa tgtactgtca tgcctagctt ttctaatatc tttaggttaa gcaaagcatc
    24661 gccactatat tcctcagggt gagcagttat ccagacagag acatcttttt ccgctgacaa
    24721 atgccgcgcc gccacaaaac catcaccacc attattgcca cagccggcta taatcatgat
    24781 tctttcccca tactggcctt ggcggattgc ctccactaga cgcagaccgg cattttccat
    24841 gagcacaatc cctgggatac catactcagc aatagccttt tgatctatcg cccgcatttc
    24901 tgccgccgtc accacattca tctcacccac ctctttatcc tcatatcctg taatgatgtt
    24961 aacgcttgcg gttgtgtgcg gggagcgtga caaggaaccg tcccctgaat cagaaaaaag
    25021 gcgcagtaaa tgcgcctttt tcttaaccac agtcaccgct gcaatctcca caaccaccac
    25081 ttcgtcccgt ttccatgcca gttatccgag ctatggtttc attgacagca gctaaaagac
    25141 tgtccacttt ctggcgtgca attaaatatg ccttaatctc ctgattctct accatctgac
    25201 gctgcagcgt ctgcagctcc tctactaatt ctggtgtaat gctctgccct tgactttgct
    25261 tattattcag ggctagttgt tgtgcttgaa agttttcgat taacgacacg gcagcgtcgc
    25321 tttgctccat gatctcttgc gcatgtgtaa agctttggta ttcatctgaa gctagaatag
    25381 attcccccag ctgttctgcc aactctaatg gatgcattta cattctcctc tcttttttta
    25441 cttgtgctta tcagtctgct gttgataatt ttttatggct tgcctcaaag catcggcagc
    25501 taggttggaa cagtgcattt taggctgcgg caagcctccc aactcctccg ccacttgctt
    25561 attagtaatt ttcaatgctt ctgtaatagt tttaccctta accatttctg tgattttact
    25621 gctggtagca atagcagcac cacagccaaa tgtccgaaat tttatttcct cgatcacatc
    25681 ctgctcattt acttttaagt aaatacgcat gatgtcgcca catttcatat tccccacttc
    25741 cccaacaccg cttgcgtttt caatttcccc gacattacgc ggatttgtga aatgatccat
    25801 tacttgttct gtatacattg ctcttacccc cgagttttac tttttgcctg catacacagg
    25861 tgacatagaa cgtagtcgtg caacaatttc tggtagaact tctaagacat aatcaatttc
    25921 ttcctctgta ttatcgtcac ctaaagtgaa acgtaatgaa ccgtgggctg tctgatggtc
    25981 taaacccata gccattaata catgtgaagg atcaagtgag ccagaggtgc aggccgaacc
    26041 actggagaca gcgattcctt tcaggtcaag acttaagatg agagactcgc cttctacata
    26101 tagaatcgag acatttagat tacctggcag gcgttgttca gggtggccat tcagcctcgt
    26161 atcttcaatg gctagaactc ccgcttttaa tttgtcccgt aagcattgta cacgcttatt
    26221 tttttctgcc atcttgctta cagctaattc tattgctctg cctagaccga caataccagg
    26281 cacattttcc gtgccgggtc taagtttgcg ctcttgcgca ccgccaaaac tatagatatt
    26341 caaacctgtc cctttacgca catataatgc accaataccc tttggtccgt aaaatttatg
    26401 cgccgaaagt gataaaagat caacttgcag ctccttaaca tcgatgggca gcttgccaac
    26461 cgcctgcaca gcatcagtat gaaaaacaat gccgcgctcc cgacaaagct tgccgatctc
    26521 tgctacgggc agaattgtcc ctacttcatt attggccatc atgacagtga ctaaaatagt
    26581 ttgctcttgc agcgcttctg tcacatcttt tttgctgacc agaccatact catctacagg
    26641 cagaatagta acttcaaaac cttccttttg cagtgctaaa caagtatcca gcaccgcatg
    26701 gtgttcgaca gcactggtaa tgatatgctt gcctttatgc tgctgcgccc gcgcaatccc
    26761 gcgaatagct agattattag actctgtggc gccagaagta aaaataattt cttgcgggtc
    26821 agcgcctaag gcggtggccg ccttttcccg tgcttcatcg accgcttttc gtgcttcgcg
    26881 accaatcgca tagacgctgg aggggttacc gaaatgttcc gttaaatacg gtttcattgc
    26941 tgcaaagact tcttcgtgca ctggagtcgt tgcggcatga tctaaataca cttttcgcat
    27001 cagttgcctc ctgtgtttat tctgccgaca tcttgattac gtcagccaga gtaatatcat
    27061 ctaacacttc agacatagaa tctcgtaaac gctcccagac tactcgtgtt aagcaagccg
    27121 cactacggcc acaagcatta ttgttgcctt caatcacaca ttctacgggt gcaatcggcc
    27181 cttcaagcgc tcgcagcaca tcacccactt tgactttatc aggtgctttt gccagaacat
    27241 aaccaccttt ggcgccacgt accgaatcaa tcaggccggc tcggcgtaat ggctgaaata
    27301 tctgctctaa aaattgaaag gaaatatctt cgcgttctgc aatttcacgt aagggtacag
    27361 gtccttcgtg gaactctaat gctaaaatag ccatcgcacg tactccatat ttacctttag
    27421 ctgaaacttt cataagtact ccacctacta atcctgatta aattactctg ctttaagagt
    27481 agcaaaattg gtagcgctag tcaagtagcc aagcatattt tttctaaatt cttatttggt
    27541 catcgcgagg gctacggctt gcgctacagc atagttgcgg ctatgactta aagaaagggc
    27601 aatcgagtgc agcgatagct ttgctgcgcg cagctttgct cgtccccgca ggcaaacagt
    27661 tggttggccg ctaggtaaag aaataatctc cacatcagtc caggcaatac tgcccagacc
    27721 gcagcctaac agtttaaaaa cagcttcttt ggccgcaaag cgagcggcca aatgccgtgc
    27781 tccataggac cgcgcggcaa gctgctcttg ttcctgcgct gtaaaaaagc gctgcaaaaa
    27841 aagttgcggg cgccgccgat aagcacgagc cacccgctca atttcaataa tatctacacc
    27901 agtatgcaag gccaatgatt ctttgttcat ctaatcattt atcctttata tcttttctca
    27961 taatactgtt tcaaatttat aaccaatacc ccataccgta cgcaaatagg taggattagc
    28021 ggggtctttt tctatttttt cccgaatacg cctaattaac acggtaaccg tgccagtatc
    28081 accaaaatat tcatttcccc acaccaaatc taacagttga tcacgactat atacaacacc
    28141 aggattatta gcaaggaccc acaacaagtc aaattccttg gccgtgaggt aaacatgttc
    28201 gcccttgagt gctacctcac gtgttttact gttaatagaa aggcgaccgt catcaaacgt
    28261 cccttctttg aggcgtggtg ccagttcctc accggcaaca cgacgcaaaa cagccttcac
    28321 gcgcagtgct aattctgagg gactaaaagg cttggttaca taatcatcta cacctaaatt
    28381 gaagccttgg gccttatctt ccaattcatc tttggccgaa agaataatca ctggggtatt
    28441 atcgccacgg ctgcgcaggg ccttacaaac ctcaaaacca tccatttcag gcatcattaa
    28501 gtcaagtaca attaaatctg gatgctcatc tgccaccttt tgcaagccct cacgtccatc
    28561 agccgcatgt atcacgcgat aggcatcctg acgtaaactc tgttggacaa tccgtctaat
    28621 tttgggctcg tcatccacca cgaggatttt agtaactttg ctcatcattg tcgcctcctt
    28681 gatcagttct ggaaaatggt atataaatcg agaactttgt acctatacca atctcacttt
    28741 gtacagaaac attgcccgat tgcagttccg ctaaagtctt aactaacgct aaccctaaac
    28801 cggtaccatt acgacgcctt ttaagtgagg aatcaccctg gcgaaacttc tcaaaaatat
    28861 gaggtaatag ttctggggcg ataccttcac cggtatcttc gatatggatg gctgctctgg
    28921 catcttctgc tgtggtaaaa acagtaaccc gaccgccttc cttggtaaac ttaatggcgt
    28981 tggagatgat gttaataatc atttgccgaa ttcgttcggg atcggcataa actaaaggca
    29041 gctctgcttt ttgtacttct agcgtgatat tctgctgggc tgctacaggc accatggtac
    29101 gccgcacaat gtacaagacg tcattcaagt caagattttc gggttctaag cgtaaatgac
    29161 ctgcttcgta tttagccata tctagaatat cactgattaa aatcattaac tgttgagaac
    29221 caatttggat atctaaaaga ttctcccgct gctcctcatt aattggtcct gctgtttcgt
    29281 caagcagcag ttcacaaaaa gctacaattg aggtcaaggg cgttcttagt tcatgcgtaa
    29341 ttgtcgccag aaactcgctt ttaaggcggt tcgtttcgct taatttttga tttagctggg
    29401 ttaataccct ttggccttcg cgtagccgta aatttgctgc ttccagctcc cgtgtacgtg
    29461 tcgccacttt acgctccatg ttttgataaa gatctttaat cgtgtcataa ggaccgagaa
    29521 gttttttacg gataataact gtgtaaataa agtagtatga cagcagcttc cataaatgac
    29581 cgaccatatt ggcgaggccg taaaccgagc tataaaaggt aaaagagatc tccgcaaata
    29641 ctgtagccaa acaagctaac agcaaatatt gatacaaatc acgatctaaa cggcgtccat
    29701 tcttaatgag aaaaccaatc gctaataaaa ttatgaaaac aattagatat tcgctaacta
    29761 ttttaaatgt tgtcaagcca acaccgggga taaaacaagc tgggaaatag cctgtaaaga
    29821 ccatcacgaa taaagagatg gccagcaggg cgatggggat caatacttta ggggttgtaa
    29881 gtgctttaga taatagcgct gctgacagta acaaggcaaa cgcttcaaaa aaacggggaa
    29941 taatccatag ttgcgtagcc aaatttgccg tatcacccgc gaagacaccc atcccttgat
    30001 aggccaaagt gtgcatgagg tcaaaaacag caacaaaaaa ataaataaag cctaaaaaat
    30061 ttagtaaatg atcattctta taggagatcc tgctggtatg catagcaatg gccgcaatac
    30121 caaaactgac cagaatgcta aaaatctcta ctacggtatg aaatagtaaa taattacgaa
    30181 agctgagacc aatcagggca gcagcaagca aaacagcagc aataatttta agatatggcg
    30241 actccattga gagttttgtc aaattctcca ctgctcctta tccgcatagt gagtactttt
    30301 tagtttcgac atgcggatag ataattcctt tttctttaaa aacgctctcc ccgcagaaac
    30361 gcccacccta tcctttgctg ctaattcctc aaaaaaatac tagccgccgc ggctagtatc
    30421 tacatttgca tgtattgtgt ttaagggata taaatccttt ctgtaagtag attgtcatga
    30481 gcaattcctt cgtaattgag cgtaacgttg gcataagcta atgttggatt atagggcgtt
    30541 acgccgaaaa gatcaagtcg tgaaattaag gaattacgca tttctaccat aaatatacca
    30601 acttgattgg tccctggcag ccaactctgg ctcccctgct tagtatgatc ctcaaaagaa
    30661 gcttcgaaat taagctctat aggattgcct aattcatcaa tttggtacat caagataatc
    30721 ttagctgtgc aaagaattgt tgagggtttc ttcgtaggtt cagtacccag ctgcagctga
    30781 agctgatagg aaaatgaacg tggcaaagcc tctaattcac gttctatcaa gagattaggc
    30841 gcaggcttaa ggaaatctgc ctccactgcc tcaattaact cgaccatctc ctccggcttt
    30901 ttgttgatca caaatatttc attgagacga gaaattgcgc caagtttttt taacccttcg
    30961 agggaaatca gttgtgcttc ttctatgtaa aaatcatagt ctgagtcttc ccactgggct
    31021 tgcacactat cgcttaagta ggtttggcaa tataccacaa gcttatccgg gtcattttct
    31081 tgcagccagt ttttagcccc gttaacatca tgttggcgtt caggcaaagc aacttcttgc
    31141 aatacctcag ttgctgtatt ttttatacca ttatatctta cgtagcgacg tatgaattgt
    31201 tttttattgg gactcatctt ttcttctaat aaaatatgat aggtgaagtc aggcaccgtg
    31261 gtaaaatcaa catcgaccag actgacaatt ttatcagcag gttcgaaatt ctcaacaaca
    31321 ggttcgagat cagcttctgt cccgcatcct gaaagtaata aaattaaaac tagtagcaag
    31381 acactacgta ttttcataat tgagctcctt ttggcatgtc tcacttcatc ataccagaaa
    31441 taagccttta ttatgttact gtttctttac ataattcagc aagaaagtta agttttttta
    31501 aaattaacta caaaattcac gatttaagct cttcattttt cattacatct actgctgcca
    31561 ggcctgaact cattaccgca ccacgcagcc aaggatgata cataaagggt aattttaaat
    31621 tttttaagac aatgttgcgc cttaaaactg cagtttccct ctggtaacga gccacaaaac
    31681 cgtcgagacc gggtgccaga cttttggctg cagctaacgc actacgaaag gcatagctaa
    31741 aaccttcagc agaactggga ctaatccaac cagcggcttc gccaattaag gcaactcccg
    31801 gtgggagggc aactaaacgg ggagcatgcc gggggcggac aatgagagct ccttcttttt
    31861 tttgcgccgt ttgcaaaggg tagccataag caacaagttt ggctttaagc tgcttaaatc
    31921 tctctactgt ctgctggcgg ggtctcaagg ctgccccaac aagcaaagag tcttctttag
    31981 gaatagacca gccatagaaa tccattagct cactatcaaa aaagacggaa aagtaaggag
    32041 aagcctcttt cacactaaac cattcttgga tagcactata aattactagt ttttggtcat
    32101 taggaaaaaa ctttcttctg acaagggagc cggcaccatc tgcgccgatc actaaacgtg
    32161 ctttctcttg ataatgtttt ccctgctgca gcaaatctag agtaatgtgg ttgtcttcct
    32221 gttgaatgtc cttacacagg gcgtcagcac gcacctcaac agttgcaggc actaaagaaa
    32281 ataaccagtg atcaaatttt gtccggtcaa gattaatata aaaccgctga taataacgtt
    32341 cccggccctg ctgcaaatca atagtacgaa caacaaaaat ttgcgggccc acaatcacct
    32401 cttggggcaa agctaaactc atcatgccca aaactttctg cgcatcagga gctaaaaggc
    32461 cgccacaggc tttagtaccg gcggcggcct gcactggaac aagtaagttc cgcctttcaa
    32521 ctaataacac ccgatattta gcagccagta aacgagccag cgttgaacct gccggtcccg
    32581 cgccgataat ggcaatatca tacactaaaa accctctccc tcaaaaattg atgcgtagta
    32641 aagcaaaaga taaccgcaaa cagtcaaaat cgttgcactg agcttaggct aaacctgacg
    32701 caagatggtg accagttcgg caacggaatg agcgatatgt gtagggtttt ctgcctgcag
    32761 ctcctcctcc tgaccataac cgtaggtcac agcaatggca tcaatgccct gagcattagc
    32821 accaaccaca tcaaacatgc ggtctcctac catcaccaca gcttggcgtt ctgcttgagg
    32881 gatatcctgt aagatgtcag caataatttc tgctttcgcg gtgcgcgtac cgtcaggatg
    32941 accgccgaca atttttgtaa agtaggtgtc cagtgcaaaa tgttttaaga taagttctgc
    33001 aaaggccgtc attttggtgg tagccacata taattttctg ccgtcagcac gtaaatcagc
    33061 caacatctcc ctgatgccgg gaaaaacttt attttcatac agaccactga caccatgatg
    33121 ctcacgataa atagcgattg cttgatctac ttgttcttca ctgaaggcat acatggtgcg
    33181 gaaagaatcc ttcagcggcg ggccaataaa aagacgaaat ttttcttcgt cttcttcatc
    33241 cacgcccatc tgcgccaaag catgacgcag ggagctgata atgcctgctg ccggatcagt
    33301 taaggtgccg tctaaatcaa ataaaatatg tttatacatg cctagattct cctctgataa
    33361 ttttagtctc gctgcttggg cgggttaggt ccgtggtatt gataataatg acattcaata
    33421 cggccgttat agagtttgcg ctttttattg gccggacggc caaagatttt ttcaaaacct
    33481 ttatgcgagg taatgacaaa gaaagaccaa gtaggtaatt ggccaaaaac ctgccccatt
    33541 tcacggtata atgcttctgc ttcctgtaca tctcctaaac gttcgccgta gggggggttg
    33601 cagattacat agccgtaatc aaaacgagaa cgaaaatcag tcagtgctaa cctctgaaaa
    33661 tgaatgtgac tttccacacc tgctttacgg gcatggtggc gggctaaact taacacttga
    33721 tcatcatggt ctgtacctaa aatacgcaga gtgcggtctc tgacgatcag gtcctgtgcc
    33781 tcagtacggg cagtctgcca caaatggtga ggaatattag gccacttttc ggcactaaaa
    33841 tgccgcttta aacctggtgc gatatttagg cccaacagag cagcttcaat agggatggta
    33901 cccgaaccac agagagggtc agccagtacg cgatcagccg gccatttact aagacgtacc
    33961 aaagcagccg ctaaagtttc tcgtaagggt gctttagcgg ttagttcacg gtagcctctt
    34021 ttatgtaagc ccgcaccact agtatctatg gtcagagtag cccggtcttt tagcaaagcc
    34081 acttcaactt tatatttagg tcccgtttcc tcaaaccaag tctgctgata gttttgtttt
    34141 agcttttcca caattgcttt cttgacgatt gcctgacagt cagaaacact aaacagtttt
    34201 gatttaatcg atttaccatc tactggaaat tctgcatctt gcggcagcca ctcctgccac
    34261 ggcaaagctt tcgtctgttc aaatagttgc tcaaatgtag tggctataaa ttcaccaact
    34321 ttaactaaca cccggtcagc tgagcgcagc cacaagtttg tgcgacaaat tgcttcctca
    34381 tcgccataaa aggtcacccg accgttttct accatttgat ctcgataccc taatgctttt
    34441 aattctttag ccacaattgc ttctaaaccg aaagccgcag ttgcaattag ttcaattttc
    34501 gccattacct actcctttac cttgattgtg tgacctcttc tacaaagcca tctctgcatc
    34561 atttattgat aatggcctgc cagggtttcg tctgtatttt atcctggtta tctgcgcttc
    34621 gctaactgcg aagtactgcc attgctctta agtataacaa aacactctac atttttccaa
    34681 cattacactg acactcttct cgctgcgaag atgaccatct ttagcgtacg cagaaaaatg
    34741 ctgcttaatt ctggcttgta agcgaagtgg tgttgtttga tttctctccg cgtgaaaacg
    34801 cctgacggta atgagcctgt caggcgtttt gtttgtttaa ggggcagtct attaatgtaa
    34861 gagtgcactt tctacagctt tcagaattac tctactgcta taggttgctc cggacaccgt
    34921 atcaacctgt aatgattgtt gtgtgataat ctcatcgaca atactttccg ctgcctcacc
    34981 tttaccatga tcatgcttaa taagcttaat ctcagcgatc tgagcatctt gcacaataac
    35041 atcaactatt acttgaatag gaccggcggc cacgtcacct tgataaacac catcagcaat
    35101 gttagctagg ttaacatctg caaagacgaa atcattaatc tgctgtatcc ctcgccaaaa
    35161 agcaaaaaac atgcctccac caactattaa caaagcaatt aagaaaaaca ataagacttt
    35221 gcgcagcatt gttttacacc tccttttttt atgactgctt cctacagctc cccacaagcg
    35281 taattacgca gaaaaggtac ctacgaactt tttctataat cacactataa caaaaattcc
    35341 ccggacatta aagggaatta tgcaagcact atactgtttt tcaccaggac aagaagagcc
    35401 aggccaagtt aaagtggcct ggctaactct cacctattca ctttgctcct cgacaatttc
    35461 ttcattacgt tccacagggg cgtctaaaat tacatgctca atatttaagg gcttaccgtc
    35521 taccgtaatg gccactaaat ctaccccttc aaactctgtc aaagtgttga caatcgaggt
    35581 aatagtcatt gtttccccgg cggcaccacc gctgtgttta gtatgcattt ccggggaaaa
    35641 atcaactaca gcaatgccat cttgcatgct cacagagtgt acctgtactt ctgcaggaat
    35701 agtcggcgct aaattgtttc cttgagggcc acgaatcaac tctgccaaca ctatccgtaa
    35761 gcggtcagct aaatcagcag ataaggcaaa actaagcgta cgcttttctg ggatcaaggc
    35821 tgtcgcttga ggattagcaa agtaaagggt tacttcttct tccgcagtcg gtgaaccacc
    35881 atctgcggta aaatgcgtca tgaatccccg cgcctcacca ctaggtccaa tgagctcgct
    35941 gccttctatc aggatcttaa ctttctcaat ctctgcggac tcagttaaag tattgacaac
    36001 ggaagcaacg gcgataattt cagccacact ccccccaaag gaaagaaagt gctctgttaa
    36061 attaactagc aatgcgttat cctgacgcat tgttccatag acttttgtcc ctgcaggtat
    36121 attcctggta aaacctgctg tttcagggcc agcaatgagc gcctgcagcg ctgctttgta
    36181 gcggtcttct tcctcttgaa aggtaatctg cctttcttca cttactaact tctcattatt
    36241 ggcatcaccg tagtacaatg tcacattagc ggtaatttcc tcgggctcta tcggtggtga
    36301 ttccttattt tgcgcacatc cacacaatag cagtaacaag acaagtaccc atactagctt
    36361 tcgcatcatc tccacccttc ttagcttacg atagtagttc cttgttctat tttgggttaa
    36421 tctagagtat ttatgccact ttctgcaggc tgcaagcttt aaataagctt gcagtcttta
    36481 tttttgcttg ccagacagta aaataaaaga tgtgacacac agccacatcc tttatcctct
    36541 ataattccca gcggtcataa tgaacacatt gcgggtcata acgatctccc actgcttttt
    36601 cttctgcggg ataacccaga gctaccacag caagggggat aacatgttct ggcattccca
    36661 gatatgtacg taatttgcct actttttcgt cattgggata aatacttagc caaacagcac
    36721 ccaacccgct ggcatgagcc gctaaaagca agttttccgt ggccgcagca caatcctgaa
    36781 cccataatcc ggggaccaac tctttctcta aatcaccaca aaccacgata gccgccggag
    36841 cccctgctaa gacggcagta ttcggagcac agtcccgcat accagctagt attgcccgct
    36901 cccgcacaac aataaattgc cagggacgag cattccgacc cgtgggggca gacatggctg
    36961 cccccagcag cgcggtaagc tgttcagttg tcagtccttg ttctgtaaat ttgcggatac
    37021 tccggcgagt gagaatattc ttcactaaat ccgctccttt cttcgatacc attaattact
    37081 ctatcactac agcggtacca gaggctgtaa ccattaacat attgccaccc tgacccagca
    37141 cctcataatc aatatctacg ccgactacag cattggcgcc aaccgctgct gctcgtttaa
    37201 ccatctcttc aattgccatt tccctggcct caattaattc cccttcatag gcaccggcac
    37261 gcccaccaaa aaaattacgc aagccggcgg aaaagtcttt aataaagtca actccggaaa
    37321 ttacctcccc aaacataatc ccgcgatact ccagaatttt acgaccctca ataatagggg
    37381 ttgttgtaac caacatcttg gcaccctcct cctgacagcg acagattttg cagttctttt
    37441 agcattctat accgatttaa gttaaaaata acctcggcag cggctcttat ttctgcacga
    37501 ggttattatt atgtttagct agccactctt tctaatttaa atataacagg atgcagccca
    37561 tcgttgcagc tggagatagc aataccctct tctctggtat aaggactggt gcctcccgca
    37621 gccaacagct ctacagttgc agccagaccc cgccaagcac ctggacaaaa atctgccggc
    37681 atttcaccat taaccgcaag gtagctcaaa ccttcctcgc ctttagcaca aactacgggt
    37741 acagtttctt tcgcataggc actatgaaca tcttctactt tcgtcttctt gaggatggta
    37801 atccttactt tggctttctt ttcttctgtc attctgatac ctccgattgt gaatattctg
    37861 tgttttaatt tattatatct tgtcacaatt caatgtacaa gatataaata actagttaaa
    37921 tatttaacaa tagctgcatt tagatgtagc ttaaccatag tagtgattaa gggacaagat
    37981 taagcccctg ctctacttct acttccactt gcaaatcttt gggcacttga atgacgactg
    38041 cttgcgcacc taacagtgaa ctcatattcc agtgccagtt gttactgctg tcactaaact
    38101 gctgcatcgt ccaatccttt gtaaaggcaa aggcaaccat ttcaaattgt gctgcattgg
    38161 ttaattccaa tttattatct atccgtctaa cgctggggga attacccaac agatccccct
    38221 taaactttaa actggtggta tagctggtta catcaatttt accatcattg atttcttttc
    38281 tttcaatggc cacaagcggc tcaaaatact ggtcggcagc agttgtgaga taaagcacgt
    38341 catcgtagta agtaaaattc cagtatccgt taatctgtat tccctccagc ttactcaggc
    38401 ggcctttctc cagtgcggca taaaactgca attgcatggc ttcagcttca gcttccgcaa
    38461 cttgtatatc ttgcgctgac gctgcttcct tggcgtcagc gacaagataa aaggcacctg
    38521 taaagatcaa cagcacagct aaatacagac taacagtgat ccgagtttgc cgaaaagaaa
    38581 cacgggctgt gcgggcaatg cctgaaaaaa tacccagcaa aatcaagatt gacggccata
    38641 caataagtat acctagatta ttcatcgcct gacctccatt ctgttcgtaa agaggatcac
    38701 tgtggcaaat agcaaggctg ctgcgcacac tactttcatg ctaaaaagga gcattgaagt
    38761 ttcgcgtaca aaaaacacaa agatcgttgt taaaagctgc acctctccag ccctcctggc
    38821 ttctataaac aatgaaccta aaaaaagcac aggtattaca atcgttaagg ctttattacg
    38881 ctgagttagc atgccaaaaa aatatcccag cacagatagt aaaaaagcat aaagtgctgt
    38941 cacataacta ccactaagta gtgttaaagg ggcaagatac aagctgttgt tggtatatac
    39001 gtcttgaaac caatagacta aaagtcttaa caatactccg gaaagtgttg ctgtaatccc
    39061 cccgaccagg gcagctgtcg ctaacagaca aacactggca agattactgg tcaagcgagt
    39121 ggagacaaaa gaaaaatcta gtcggcaagc attcccagcc accgtattgc caatgatgaa
    39181 ggcccagatc aaggtaaata caataataat atctcctgaa attagtttaa gattaatcac
    39241 catcgaacta gacccggctc ccatggaggc agaaccattt aaagatagca gcagcgccaa
    39301 caattgaaca atcattaaac taaaaaagat acccagattt gttttgagct tataagcgta
    39361 ttgttttttg catactgcta gcagcttagg ttcgtgaaaa gacattatca attcctcctt
    39421 tatgctgggc tgtgagatag atggacaaat catcagttgc caccggggaa atctcaaccc
    39481 cctgtaaacg tgcttgctgt aatatctcct ggttaaaagt gttacgggct accaaataaa
    39541 tactgtcttt gccgagttgc cgttgatata gtatttgctc cggcgtgatc attttgttga
    39601 taatgcttgc ttgaccagta aaaccgacag catagccttt taattcatca agaggtaagt
    39661 gtaaatactt ttcaccagct ttaatcagca gcaaatcatc aagcagctcc tcaatctcac
    39721 ttaagagatg actagagaaa ataatcgtgc gggggtactg catgaagtct ttaagcagcg
    39781 cccgataaaa atctttgcgc accgccacat ccatgcccac agtcggctca tcaaaaatgg
    39841 tgagcgggca gcgggcggca atgccaataa tcatctttaa actgcttttc atgcctttag
    39901 aaagattagc ataatattgt tgcggctgca gagaaaaata atctagcaat ccccttgcta
    39961 attctgcccg ccagttagcg taaaactgac caatggctgc taatgtctcc cctaaattaa
    40021 gattatctgg cagttccagt tgatcatcaa taaaaattag attggcagcg acatgtaaat
    40081 tattaaaggg agtttggcca aagacgcgca cttctcccga gctgggcagc agatagccgg
    40141 ccagtagttt taaaagggtg gttttccccg caccgtttct gccaaccagt ccggtaattt
    40201 tattttcttc tatgctaaag cttaagttgt taacagcttt aaacttgcca aatgatttgg
    40261 caagactctc gctctcaata caaatcacaa ctattccccc tctgcaaaac tttgttctgc
    40321 catttcgatc atcctgacta attcctcacg gggaacatgt aaacgtcctg cttcggcaac
    40381 aacttccaag accagctgct gcaaactttc attaattcgt ttggtacgta ttctttcttt
    40441 cgcctgtggc gcaacgaaca tacctaatcc ccgtttcttg tataaaaggt tttcctcgac
    40501 caacatggac agccctttag cggcagtagc tggattaata ttaaacatct ctgctagctg
    40561 gtactgtgaa tagatttttt catcactggc aatggagcca cgtaatattt ccagctccag
    40621 ccattcagca atctgcatat agatcggttt caaactgtcc ggattaagta acactcatac
    40681 cactccctcc atcaccacaa caatagtgca ttactactgt aatgtagtat ataatattag
    40741 ttgaactttg tcaagaggtt tgtattaaaa aatgccccca ggaggagcaa aagttatttc
    40801 ggccactaag ttgtctgggc tattatttat ttgtctccct taagtagtca acaattaatt
    40861 tatccaactt ttcactttgc ttaattgtct gattatggtt gataccaaac ctgttaatta
    40921 ttgctgctaa acgttgacgt tctctttcca ccttttgctg cagctcttcg tacatataat
    40981 caaaatcacc ccgttctgca agagagccta atcccatatt tcgataagct taaacatatt
    41041 tcaccaataa ttttattact taattgcagc tcatttacag taatgtattt gtcttcctct
    41101 atcctaccaa attataccat ttgcagcaat acatccatcg acgtatcttt taacaaaatc
    41161 agtgtaaaaa ataagtctat cggcttattt ttttgctagg tagtacaata cagcttccat
    41221 tctatccttt accgctaatt catgtagaat attagaaaca tgaactttta ttgttttttc
    41281 acttatttgt aattcagcag ctatttggcg atttgttttg gcctccgcta atttttttaa
    41341 cacctccagt tgacggcgcg ttaatcgtgt caatttattg gccggctcca gatgcattaa
    41401 ggactcctca atgctggctg ttagctgctc agtcgtcata ctatttaatt gtctgctgag
    41461 ctcttttatt tgatactctc tgacaattag ttctcccagg atactagtca ttgtttgcag
    41521 ttgagttagc tgtctttgcc cattgggata cagattatga gttaaaagca gctgccccat
    41581 ttcttggcca caagcgcttt ccaattgctg ctcttgcttt ggttttataa aatgacctgt
    41641 aaataaataa aaaggctcct tcttgatcct aattttagtg taaataaact caacaccatc
    41701 taaatcagct tgactggaga tcagggttcc actatttatc acaaacgctt gataaaatcg
    41761 caaaagtagc tcttgcttgc cttgattatt aaagtaaggt gaagcataaa taatctcgcc
    41821 ctgtttgtcc agcagcaata agggcacttt aataatttca gagattatat ccacaagtgc
    41881 aagaatagac actccatcca tatgtacctt ccttattatg gcaacccatt attttctagt
    41941 catgatctgc tggaaataag caccaagaac tttaagagca gaaaggagta gatttgtaac
    42001 caatggatac aatctactcc tcacattaat atatcttaag tactaatcag ctaaatttgt
    42061 cactaatcca ttatttaacg cttgtttagc ttatattctc ttaactatta ccttgtaagc
    42121 caatactttc tcctaccttt tgcatgccct tgagcgtttc catcattaca tcgtctaact
    42181 ccatacccag cagtttagca ccttcctcaa caacttctct gtttacgcct gcagcaaaac
    42241 tcttttgttt ccattttttc ttcacagatt ttaggtttgt atcaaagata cttttactgg
    42301 gccgcattaa agcagtagcg gtgatcaagc ctgttagttc atcaatagta tataatactt
    42361 tttccatgcg ttcttgtggc tccacttcag tacaaagctt ccagccatgg ctgatcacag
    42421 catgaatata atcttccggc cagttgtttg ctcgcagaat ctcttctgtt ttctggcagt
    42481 gctgttctgg atacatttca taatcaagat catggatgag gcctataatt ccccattttt
    42541 cctgatcttc attaaataat tcagcgaaat gaaccattac accttccaca gttaaagcat
    42601 gtttaattaa gctgtcgctt ttattatact ctgtgagtaa gttataggtt gcttcccgtg
    42661 ttggagttac tgtagtcatg taaaaacctc tttcctatat gtagtaaaat ttccctatgc
    42721 ctttaaattt taccttctcc tacagatttg tcaagttaag ggtggcagtt cctttgctga
    42781 tcatcattcc agcaatattt ttaagatctt ctgcctatta ttgagaaaac ttcgcatgat
    42841 ttgataaggc tatgtttctt cgaagatctc cccttcattg tataccaatc tgactgattt
    42901 tcccagagtt gccttgcaga aacagctgct ctatgcggct ttctacaggt acacagtctt
    42961 ctctaagggc tacactttaa cttttgttaa ccaatcttta agcaagactt aactgcctat
    43021 taagcaggca cactggatat aatatgacaa gctgcttgca caggtctggg aaaagctgaa
    43081 atttgcgcta gaggtgtcgt gatgtgaaga taggcaaccg aacacagcag ggattactag
    43141 caggtttatt cgctgggata gtaatggata tcaccgacca attgacctat ctcctggtta
    43201 aggaacatac attgcgttct gcagactggg tggcactgct gatctatgga cggcaaagca
    43261 atcatgattt agaattactg ttagcacaag tcatacattt tctgtttact gccatgcttg
    43321 gcttactgta tgtatatttt atcgccccca taaaaattaa aaaccacctt tttaaaggct
    43381 ggctatggag cacttttgtt tggtttgtgg ccatcacgct aaccatttcc actaaaatac
    43441 cagcattaac agaaattgat gtgaacgatt ttatttttca ttttctgctg gcctcaattt
    43501 acggactaac gctggcagca gctaatgcta ggcttgctaa ataaagcagc cctttaggct
    43561 gctttattta aatgctgtca ataatgtttt tacgtttctg ctgaaactct gtcgctgaga
    43621 ttaaaccctt atcgtacaga ttttgaagtt cttgtaagcg ctgttctggc tccagcctct
    43681 cctcattgtt tgttacctgg agttcaggag catcactaat cataatttca tgtgagacta
    43741 ttgctttatt cgtgaaagca ttaataccat tgactacagt aatgatcaca gccattattg
    43801 tccaaaagat gccaaacagc cccgctgttg gtatcacgat aaaaaaccct ataccacaga
    43861 acaccaggcc ggcaaaaaaa ccgattgtcg attgtccttt gcctggttta acttttatgt
    43921 ttttacccat tagaccctcc tgataatcta aaacttagta gtacaacttt tatagagcat
    43981 gctcttctca ttataacaca tctgccgcta gggaggccgg atggtgcaca caacttttcc
    44041 cgctgaactg cattaaaatt atgaaacaat caagtccagg ttcgcacctg gacttgattg
    44101 aagataagct cttgcttaaa gatactgggc agcctcttta tcaataataa atgtgcagtc
    44161 gtcatgcagc tgcaggatgg aagctgggac gtgaggattt atttcaccca gaatagtatc
    44221 ttttaccgcc tgcgctttac ctgctccatt tgctaaaagc agtatctttt gcgcctgcat
    44281 aattgttttt atacccatgg taatggcttg ggtaggcacc tctgttctag agtcaaagaa
    44341 tcgagcgtta gcataaatgg tatcctcatc tagctgcacc aagtgtgtat tgccaataaa
    44401 ttcaggagcc ggctcattaa aaccaatgtg gccgtttctg ccgataccta atacctgtag
    44461 atctaaaccc ccggcttgtg caatggcttg ctcatagcgc agacattcct cgtctgtctt
    44521 agccgccagc ccattgggta tgttgatatt ttcctgtgtg acattaaggt gcttaaaaag
    44581 attatcctgc atgaagtaat gataactctg ttcgctctca ggggatagtc cgaaatattc
    44641 atccaaatta aaagaaataa tttggctaaa gtctaaagct tgctgctgat gcattttaac
    44701 tagctcttta tacataccaa ttggcgtgct gccagtacag agaccgagca tgctattagc
    44761 ttttagtgct acttgacccg ccacgattag ggcagcttta acactcaatt cttcatagtt
    44821 atcgacaaca tatattctca tcttaaacct ccctcggttt atcctacaac actttacctc
    44881 ttctgcttct tgtcagcaga aatttacaga cactaaaaag acacccgagc tctttgtccc
    44941 gaaaattact tctcggtttt gtaagctctt tatcggtcgt gactcaaccg ggaggcatcc
    45001 gccgaatatt cgatcaacct ccacctcgtc aacacctctt cgtgtgttct ggcgctaata
    45061 ttcacttgtg gttttcatcc ctccttagtc cagttcgtct tgaataacgg tgaccaaatc
    45121 atcagccagc tgcagtaatt ctgcttcatt tgttccttcc agcatgacac ggattagcgg
    45181 ttcagtacca gaaggacgaa taagcaagcg gccaacgccg gccagttgag cttccgtttg
    45241 cgccacagct tccttaatac acgggttttt atcccaacca tcacgtctgc cgacccggca
    45301 gtttactaaa acttgcggat atcgtttgta ggcagcagca agttctgcaa atgactgttt
    45361 cttcttggcc gctaccgccg ccactagcag tgcagtgagt aagccgtcac cagtggtgtt
    45421 atgctgcagc aaaataatat gacccgattg ttcaccacca aaactaaagt ccccagccag
    45481 cattgcttcc agtacatagc ggtcgccgac attagtgcgg acaagttcaa aaccgtgagc
    45541 ttgtgcggct ttctctaggc ctaaattact catcaccgta gcaacaacag tattgtgttt
    45601 tagctggcct ttttcctgca agtcttgcgc taaaatggtc ataattacat cgccgtcaag
    45661 cagctgcccg tgaccgtcca ccgctaaaac acgatccgca tctccatcaa aggtaaaccc
    45721 aaaatctgct tggtgcatct gaaccatagc agcaacagct tcaggatgtg tagaaccgca
    45781 ctggtggtta atattgatgc cgtcaggagc atcgtaacag gcgatcactt ctgcaccaag
    45841 tgctctaaag gcctgcggtg ccaagttgtg cgctgaccca ttagcacaat ccaacactac
    45901 tcgataacca tccaagcggc aggatactgt agaaagtaca tactgcaaat aacgttcgcc
    45961 ggctgttatg tcttggaaaa cacggccgat agcagcagct tctgggcgcg gcaggtcatc
    46021 agtttcttgg aaatataatg cttcaatttc atcttccact tcatcaggca gcttgaagcc
    46081 ctcagcacta aaaaacttaa tcccgttatc aggataagga ttatgggaag cagaaatcat
    46141 cacaccacca caaccattta gctgctttgt taaatacgct gccgcaggtg tagaaataac
    46201 accagctaca tgcacatcaa caccagccga ggtaatgccc gcaattaaag caccctccag
    46261 catctggcca gaaacacgcg tatcgcgagc aattaaaaag gccttgtggc ctttgctttt
    46321 ctgctgcaaa agataggcac caatccgacc aattctaaac gccagctctg gtgttagctc
    46381 tttatttgct acgccacgaa taccatctgt accaaacaat ttccccatgt tgtcacccct
    46441 ttttaaagcc tttttagtat agcataactg tgtcctattt acaaaccact aatgcctgac
    46501 atataaacca ttagcattat acgcctaagg ggaggattcc gtgtgtagaa cacccttaac
    46561 aacatcatca ggctggtctc ccagcctgat gatattaagt agctatgatc aagatagcgc
    46621 atagagcacc ctattttttc ctagtcaacc tctgccgtaa ttattatttc cgctttaatc
    46681 tggccttcaa ataacaattt tacctcttga ggaatctcca gctgcagatt aagcgtcgtg
    46741 ctttcagtca aattgctgag gtcgacagtt tcagtgctga gttcagcaac ggtagttaag
    46801 tattctgctg aaccttcaat aattacagtg gtggggtcta gttttatttg ctgtaccgcg
    46861 taaccagctg gcggttttcc taccgtagca acttttactg aaacttcttt ttgaggttcc
    46921 gaaataggaa taaatacttc taccatgctc gggctaattt ctatcccttc taccacctgg
    46981 cctgccctgt ccactgcctg cacaggtact acttgtgacc agtcacgggc aataccatca
    47041 atattcacaa taactcgcac gcgatccaca ttattaagac gtgtccgacc gccgcgtaca
    47101 aaaacagagc tagggttgat acgtgcctct ccccgaataa agccgctcgc cggtgccccc
    47161 gtgacgtcta ggaccacggg catgtgcggt gattccacct cttctaactc caccaccact
    47221 tgttggggat taaaagaaag cacttttact ttattaggta cttctgcagt aggagtaagc
    47281 cgatgctgac ctgcagcaag atcacgtaga tcgacaaagg ctttcaagtt gaggggggta
    47341 atattattta ggacgtcagc aggaccgctt aataccacct gtacttcttg cggcatttct
    47401 accaattcta gttcctcagc caaattaagc caagctaaag gcacatcgct atacgtacga
    47461 gtaagtaaat tttcatacat attatcacca gtaacatagg cccagaatag tagtgccatt
    47521 aaaaaagcaa taattttaag gacaatgttg tttttcagga tgcgttccat cattggctcg
    47581 acctccactg ccaaaagtta aaagaatttg acggtggttt acataaatca attaacatct
    47641 gctttagcgt tttatcatca agatagcgcg ttaatttacc gttgttagtt aaagagacta
    47701 cacctgtttc ttcggacaca ataatcgata cagcatcaga ctgttcagaa acaccaagag
    47761 ctgctcgatg gcgtgtgccc aattctttac ttaaattagg gttttctgta agcggtaaaa
    47821 aacagcctgc tgccgcgatc cggttgccgc gaatgattaa agcaccatca tgtaacggac
    47881 tacgtggtat aaaaatatta attaaaagtt ccgcagaaac gtgcccgtca attgtaatac
    47941 ctgtttctat aatttctttt aagcctgttt cacgttctaa aataataaga gcaccaattc
    48001 ttttctttac taaaacctga attgctttaa tgatttcttc cagcattaat tcaaaatcaa
    48061 catctgcata aatttgattg cttcgcgcaa agatttttcc ccggcctaaa gtttccaagg
    48121 cacgccgcag ttcaggctga aaaactatgg ggatggctac aatccaaagc gtcatcaaat
    48181 tagtcaaaag ccaatctagg gcagcaagtc ccaactctct gctcaccacg gtaatcatca
    48241 ctaataccgc cagcccttta acgagctgct ccgctcgtgt accacgaatt aaaacaatag
    48301 ctttataaaa aacaaaagtg acaataacta tgtctaaaat actgcgccag ctaaaaacac
    48361 taagcaattg cagaattcgc gtcaatagat cacctccgtt tctcaatagt aacgtcaacc
    48421 taactataag ttatctttcg ctaaaaatct ataaaaaacc tgcagtaaat ctttattaac
    48481 a
//
LOCUS       DASRMS010000094         1149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_292132, whole genome shotgun sequence.
ACCESSION   DASRMS010000094 DASRMS010000000
VERSION     DASRMS010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1149
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292132"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(737..>1149)
                     /gene="ssb"
                     /locus_tag="VFC74_04330"
     CDS             complement(737..>1149)
                     /gene="ssb"
                     /locus_tag="VFC74_04330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK24595.1"
                     /translation="NIALAYNYGRKGEDGNKPTQWLEIAVWGKQAEGLAQYLTKGKQV
                     YVVVKDVRVETFAKNDGTQSFKLVGDLADIKFASDGQQQQQQSYPQQQAQQQQGATPP
                     PGYVAPQQQPAYQQQAPQNQFQQNGVMDDDIPFN"
BASE COUNT          268 a          275 c          230 g          376 t
ORIGIN      
        1 tattagttca ccagcttgca cttgcttcag tcgcttgcgc tctggtgcgt ttaagtaatg
       61 cagaaagcca agcaggattg ctaccactaa cacgcatagc gatatttgct ttattgtttc
      121 tatcttttcg tggttcatac gctgctcctt atgtatagct acaatatagc catactggtg
      181 agttgtggct aattggtttt tgctattact ctggctctat caatagtttt tattaaagaa
      241 agctatgtcg cattcttacc ttcaccatta atcgtgtgcc atttcttgtg gcatacagca
      301 caaagccacc gcacagacaa aggaaatgca tagtcatcat ggtgagcatg tatgtcacca
      361 acaaagtcgc atctggtgca agatgtttca cgatttaact ttccagtccg tatagcatta
      421 taaactgccg tatgcgcttt atattttttt gggtaagcgg ctctgtatgc tcttaaatcc
      481 tcaatagtct gcctatttcc cctagctctg tcgtactctc gataatattc aaccttagcc
      541 tttctgtttt ctctaacgtc tttcttattg cactccttgc acttgtttac atggccgtcc
      601 gccatctgag catgcttata gaactcagac agcggcttag acttattgca tttaaaacat
      661 tctttcatat tgacctgcct ttacgattaa cgtcccaagg ctaggctacc acgattaagc
      721 ggcatttaaa agggacttag ttaaaaggga tatcatcgtc cataactcca ttttgctgaa
      781 actgattttg cggtgcttgc tgctggtaag cgggttgttg ttgtggtgca acataaccag
      841 gcgggggtgt agcgccttgt tgctgctgag cttgctgctg tggatagctc tgctgctgtt
      901 gctgctggcc gtcgctggcg aacttaatat ctgccaaatc tccgacaagc ttaaagctct
      961 gagtgccatc atttttagca aacgtctcaa cacgcacatc tttaactaca acataaacct
     1021 gcttaccttt agttaagtac tgagctagcc cttccgcctg ctttccccac acagcaatct
     1081 caagccattg agtcggcttg ttgccatctt cgcctttgcg tccgtagttg taagcaagtg
     1141 caatgttag
//
LOCUS       DASRMS010000095        11395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_224225, whole genome shotgun sequence.
ACCESSION   DASRMS010000095 DASRMS010000000
VERSION     DASRMS010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11395
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_224225"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..944)
                     /gene="miaA"
                     /locus_tag="VFC74_04335"
     CDS             complement(15..944)
                     /gene="miaA"
                     /locus_tag="VFC74_04335"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013842002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HZK24596.1"
                     /translation="MQKLIVIAGPTAVGKSAVALELAKRLQSEIISADSVQVYRGLDI
                     GTAKPSAAEQAQVPHHLLNIKDPHETYTVAEFQKDARLAIAQVHQRSKVPLLVGGTGL
                     YLRAVVWGFAFSESGINHALRKKLRSIAEINGPEVLHHQLQAVDPQTARQLHPHDLRR
                     VIRALEVYAQNRRPISEQVEKTPTKPVYDTVQFVLTRSRETLYERIEARVEAMIEAGL
                     IREVQFLLATGVSPEAKAMQSLGYKQIAAYLTGQMPLVEAIERIKRDTRRFAKRQLTW
                     FRREKEMHWINLDDLQLNEVVKNISNSLAGNYF"
     gene            complement(946..2736)
                     /gene="mutL"
                     /locus_tag="VFC74_04340"
     CDS             complement(946..2736)
                     /gene="mutL"
                     /locus_tag="VFC74_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HZK24597.1"
                     /translation="MGKIYLLDEITANKIAAGEVVERPAAVVKELVENALDAGAHHIE
                     IQITGGGLELIRVVDNGSGMAAEDVSLALQRHATSKIKTASDLDTVLSLGFRGEALPS
                     IASVSRFRLLTRRAEELTGTELIIHGGKTVRLEEAGSPVGTEIKVEDLFYNTPARLKF
                     IKSVGSETAKVTDTVQRLALAWPEVAFILTVNGKNKLITPGNGQQEDAAAQILGRQNM
                     RQMVPVKWVGSLLTLRGFVSKPSLTRANRNLQYFFVNRRPIRSPLLADALQTAYHTLL
                     PRHRFPAAVLYLELDPLTVDVNVHPTKREVRFAQERDLYRQLLSGVKEALKKVSLIGE
                     MRTPPFAAAWQERAAHFSQAELTPGFVQRKMADKPAMPPELALFNAPKQAIDIPITPP
                     EYTADEDTVSASRNNFPALRPLGQYLATYILAQSTPGDLYLIDQHAAHERILYDLIKK
                     DLSAGELPVQSILPQTFELDAISAARLQESLNIFLDLGLEFETFGNNTFILRTVPLFF
                     KDTLSQEELMELLTQHRLEQGSVGIFEKAMQLMSCKGAIKANHTLDKAEMATLITNLA
                     ATTEPHTCPHGRPTVMILTAQTVAKNFRRA"
     gene            complement(2729..5338)
                     /gene="mutS"
                     /locus_tag="VFC74_04345"
     CDS             complement(2729..5338)
                     /gene="mutS"
                     /locus_tag="VFC74_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HZK24598.1"
                     /translation="MKKETPMIRQYREIKEKNQDKILLFRVGDFYEMFFEDAKVGARE
                     LEITLTSRDKGVPLAGFPYHALNSYLGRLIERGYKVAICEQVEDPKLAKGIVKREIVQ
                     VITPGTVTDASLLDEKSNNYLVAVYQARGGIGIAAVDISTGELRLAQARGEQAAKFLH
                     DELARLRPAEILINESAQTDEVLQEALLRLDNQTILNPCRDQDFTPKRAKGALLTQFN
                     VVNLESLGCADLPYAVSAAGAAIVYLQETQQKKLNHLQKPVIYDPHGFMVLDAVTRRN
                     LELVRTIREEQKIGSLLWVLDKTCTALGARLLKRWLEQPLLDADAIEQRLNAVEEFLN
                     NFALAEQLPELLAPVYDLERLLSRVHCESATARDLVALRTTLQVIPELLHTLQTGGPC
                     LLALAARIPDLTVLTSRLTAALADEPPLALKEGNLLRDGFHPELDKLRYAISHGRTEI
                     VEMEQRERERTKIKSLKIGYNKVFGYYLEVTRTNTHLVPDDYTRKQTLANAERYITPE
                     LKEYEDTVLGAEEKISALEYELFVGLRNQVAQYTTEIQTTAAVLAELDVYQSLARVAR
                     ENRYVRPQITAAGSIEIRDGRHPVVERVVQDELFVPNDTVLEHTTQQVLLITGPNMAG
                     KSTYMRQTALIVLMAQLGSFVPARHAVIGIVDRIFTRIGAADDLVGGQSTFMVEMSEV
                     ANILNNATANSLVILDEVGRGTSTFDGISIARAVVEYLCTKLKALTLFATHYHELTDL
                     AAVYPTVKNMATSVKERGDDIVFLHKVVPGNVDHSYGIQVARLAGLPEKVLQRARQVL
                     KTLEAPVMTKQQTAAAVAETTTQLSLFAPDPALAQALNELKEADLMTTSPIEALQLMH
                     SLQQALKKGNLDG"
     gene            complement(5340..6656)
                     /gene="miaB"
                     /locus_tag="VFC74_04350"
     CDS             complement(5340..6656)
                     /gene="miaB"
                     /locus_tag="VFC74_04350"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HZK24599.1"
                     /translation="MPQKYTLTVFGCQMNERDAETLRGFLDELGYVETEDTREADLVI
                     MNTCAVREKAEAKVLGRIGRLGQLKAERPELMIAICGCMVQQEEVAQQIKDNFPFVDL
                     IFGTHNLAAFPELLQRAAASKETVLDLWSEAGDVVEGLPVSRQDGVKAWVNINYGCNN
                     FCSYCIVPYVRGRERSRKPEDILQEVTALATRGYSEVTLLGQNVNSYGKDLTAAIDFA
                     DLLARVDKETGMKRIRFMTSHPRDFNEKLARAMAEGQNICEHLHLPVQAGSNRILKMM
                     NRGYTREHYIQLVEMFRLYLPNCAITTDIIVGFPGETEEDFSDTLALLEEVSYDAAFT
                     FLYSPRSGTPAAAMPGQIPNEVKKERFQRLLTLQNKHSLYHNQQLVGKKVEVLVEGPS
                     KTDAAVLTGRTRGSKTVNFTGGKIASGTLVEVKITEAKTWSLLGRN"
     gene            complement(6863..8260)
                     /locus_tag="VFC74_04355"
     CDS             complement(6863..8260)
                     /locus_tag="VFC74_04355"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24600.1"
                     /translation="MEKEKSKIKNSEIMAYALPGAVSAMVHQPSTQYGMLFMTEYLGI
                     PAAAMGTAMLVAKLLDYVVSIFAGSIIEKTHSKKHGKYALWMRLTTYTLFFGSLMQLV
                     NTTGFISSSLGRLLIVSIFYCTLHFGMNFRATSQGGILQRMAGTSMEDRKRLNARQAQ
                     IAAAVTIISGATTIPLIQFFGKQFGEANGYAVMVLTFGTVFLLLSYFLLFPIMKKYDL
                     PREQAEVAKAPSVGNMFKSIITNRQMVVMFLLTTATTVGSQIYTPLLAYYFRVVTGQF
                     SVMALLLTIRGVVGFLFSLVAPSIAKKVGKKGALILGSVLTIISYLGMWLLGLNYLWA
                     MVVFACLIQGAISINACFRVNYFLDCGEYGYYATGQDNRTMALTVMNWPIKIGFLVGG
                     SAVGFGLALIGYTAGMEITAEFASKFMTFMGLVPAAFSVIALFLTIFGYKLTDEQAEF
                     YATENHKREAALKQI"
     gene            complement(8501..8671)
                     /locus_tag="VFC74_04360"
     CDS             complement(8501..8671)
                     /locus_tag="VFC74_04360"
                     /inference="COORDINATES: protein motif:HMM:NF019751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type small acid-soluble spore protein"
                     /protein_id="HZK24601.1"
                     /translation="MDIERAEELLNSPAKIEVHFQGKPVWIEGVEETTANVSVMGTCR
                     TMKVPFTELEEK"
     gene            complement(8752..9216)
                     /locus_tag="VFC74_04365"
     CDS             complement(8752..9216)
                     /locus_tag="VFC74_04365"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HZK24602.1"
                     /translation="MPYARKLQAYIQEQQLAAEHLCFTESCHSVAEAAAAVGASSDEF
                     VKNICLIGSDEKLVVAIVRGKDRVDRKKAAQVLGIKSMRMATADEILERTNFPCGGTP
                     SFGFKAIFLMDKRIFTLTEVYTGGGSENSLVKAAPQVLLDANQAEICDIIKI"
     gene            complement(9221..10378)
                     /locus_tag="VFC74_04370"
     CDS             complement(9221..10378)
                     /locus_tag="VFC74_04370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HZK24603.1"
                     /translation="MKEIYADHAATTFPRPPQVAEAMVSYIRDIGCSPGRGGYRRALN
                     AARLVYETRTILADFFNVPQPEQIIFTPSVTYALNLVIKGLLTAGDHVLISSMEHNAV
                     VRPLTRLAAERKISVERIACQADGTLEPNQIKRALRKNTRLVILTHASNVTGTIMPVY
                     EIGEILAGTNTLYCVDAAQSAGTEEINFAELQCDYLAFTGHKGLLGPPGIGGLCLSER
                     AAALTKPLVEGGTGSRSEEEQQPEFLPDKFESGTQNAPGIAGLAAGVKTIQQIGLTSI
                     KQQKEELTAAFLYGLAGLPQIQVYGTNDPSTTTATVSLNLQGVDNGDLSYLLEQGYGI
                     MTRSGLHCAPLAHQTIGSFPVGTLRFSFGHQNTVEEVTEIILALGEIIKER"
     gene            complement(10359..11288)
                     /locus_tag="VFC74_04375"
     CDS             complement(10359..11288)
                     /locus_tag="VFC74_04375"
                     /inference="COORDINATES: protein motif:HMM:NF040786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism-associated LysR family
                     transcriptional regulator"
                     /protein_id="HZK24604.1"
                     /translation="MEFYQLEAFVMVAAHRSFSRAAEQLYLSQPTISSHVKSLETELN
                     TPLFDRGKNGLLLTPAGTTLFRYAKDLLQLRTDALTELGAGVIQEEKLIVAASSVPCQ
                     YLLPKAIVDFQQLYPQVKVTLRQENSRKASEDVFHYHYALGIVGEQLSIPHLVYEPLV
                     SDQLVVAIPQQAAYEELLMKETLTMADLLNYNLLLREAGSGTRSLFTRELQCNGMDLA
                     DLQLQIFDNQETIKQAVRHGLGVTIISRYVVEDYLQFGLVATRHLQDLQLTRDFYLVA
                     HAKRILTPAAIAFRDFVLSFFQRGPEKDERNIC"
BASE COUNT         3071 a         2713 c         2386 g         3225 t
ORIGIN      
        1 gttttattct ctaactaaaa gtaatttcct gccaatgagt tagaaatatt ttttactacc
       61 tcatttaact gcagatcatc aagattaatc cagtgcattt ctttctctcg cctaaaccat
      121 gttaactggc gcttggcaaa acgcctggta tcacgtttga tccgttcaat tgcctctacc
      181 aaaggcattt gtccagttaa atatgccgct atttgcttat agcccaaact ttgcattgct
      241 ttagcctcag gtgaaacccc tgtggctagt aaaaattgta cttctcgtat caaacctgct
      301 tctatcatgg cctccactcg tgcttcaatg cgctcgtaaa gagtttcacg tgacctggtc
      361 aacacaaatt gtactgtatc atagactggt ttggtcggcg ttttctcaac ttgttcagag
      421 atgggacggc gattttgtgc atagacttcc agagcccgaa taacacgacg caagtcatgg
      481 ggatgaagct gacgcgctgt ttgtggatca actgcttgca gttggtgatg gagaacttct
      541 ggcccattaa tttctgcgat actacgtagt ttttttcgca gtgcatgatt gataccgctt
      601 tcactaaaag caaaacccca cacgacagcc cgtaaataaa gaccggttcc ccccactaat
      661 aagggtactt tgctacgctg gtgtacttga gctatagcca agcgggcatc tttttgaaat
      721 tcggccacgg tgtatgtctc gtggggatct ttaatgttaa gtaagtggtg aggtacctgt
      781 gcttgttcag ccgcacttgg tttagcggta ccgatatcta aaccacgata tacctggaca
      841 gaatcagcag aaatgatttc actttgcagt cgtttagcta gttccagcgc tacagccgat
      901 ttcccaactg cagtgggtcc ggcgatcaca atcaatttct gcatgctatg ccctccggaa
      961 gtttttcgcc actgtttgtg ccgtgaggat catcactgtc gggcgaccgt ggggacaggt
     1021 atggggttcg gtggtagcag ccagatttgt gattaaagta gccatttctg ccttatctag
     1081 tgtatgattt gccttgatgg cacctttaca ggacattaac tgcatcgcct tttcaaagat
     1141 tcccacactg ccttgttcaa gtctatgctg tgtcaatagc tccattagtt cttcttggga
     1201 taaagtatct ttaaaaaata gaggaactgt acggaggata aaggtattat tgccaaatgt
     1261 ttcaaattct aatcctaaat caaggaaaat atttagcgac tcttgtagtc gagcggcaga
     1321 aatagcatct agttcgaaag tctgcggcag aatgctttgt actggtaatt cgccggcaga
     1381 aagatccttt tttatcagat catagagaat acgctcatga gccgcatgtt gatcgataag
     1441 gtacaaatct cctggtgttg actgtgctag aatgtaagtc gccagatatt gccctaaggg
     1501 acgtaaggcc ggaaagttat ttctgctggc actaacggta tcctcgtcag ctgtatactc
     1561 cggaggagta attggtatat ctatcgcctg tttaggagca ttaaataagg ctagttcggg
     1621 cggcatagct ggtttatctg ccatcttgcg ctgcacaaag cccggagtca gctctgcttg
     1681 tgaaaaatgc gccgcccttt cctgccaggc cgctgcaaat ggcggtgtgc gcatttcacc
     1741 gattagcgac acctttttta gtgcttcttt cacaccagac aataattggc ggtacaaatc
     1801 acgttcttgg gcaaagcgca cctcacgttt cgtagggtga acatttacat caactgttaa
     1861 gggatctagt tctagataga gcactgcagc tggaaaacgg tggcgcggca gcagagtatg
     1921 ataagcggtt tgcagggcat cagccagcag tggtgagcgt atggggcgac ggttaacaaa
     1981 aaagtattgg aggttgcgat tcgctcgcgt taaagacggt ttagacacaa agccacgcag
     2041 cgttaacagt gaccctaccc actttacagg caccatctgt cgcatatttt gccgacctaa
     2101 aatttgtgca gctgcatctt cctgctggcc attgcctggt gtgattagct tatttttccc
     2161 gttgacagtc aaaataaaag ccacctctgg ccaagctaaa gccaagcgct gcacagtatc
     2221 tgttactttt gctgtttccg aaccaacact tttaataaac ttaagacgtg ccggcgtatt
     2281 ataaaataaa tcctctactt taatttctgt accaaccggg ctgccagcct cttcaagacg
     2341 tacagtcttt cccccatgta taattaattc ggtaccggta agttcctctg ctcgacgcgt
     2401 caaaaggcga aagcggctca ctgaggctat acttggtagt gcttcgccac gaaaacccag
     2461 cgatagtaca gtatcgaggt cagaggcagt tttaatttta ctggtggcat gccgctgtaa
     2521 ggctagggat acatcttctg ctgccatgcc gctgccatta tcaacaacac gaatcaactc
     2581 tagaccgcca ccggtaatct gtatctcaat atggtgagcg ccggcatcta aagcattttc
     2641 cactaattct ttgactacag ccgctggccg ctccacaact tctcccgctg ctattttatt
     2701 ggcggtaatt tcatctagca aatatatttt acccatcaag attccccttt tttaatgctt
     2761 gctgcaagct atgcatcaac tgcagtgctt caatgggact ggtggtcatc agatcagctt
     2821 ctttaagctc attaagggcc tgtgcgagcg caggatcggg tgcaaacaaa cttaactgtg
     2881 tagttgtctc ggccaccgca gccgccgtct gctgtttagt catcactggt gcttccagcg
     2941 ttttcagcac ctggcgagct cgctgcagca ctttctccgg taaaccagca agtcgtgcca
     3001 cttgaatgcc ataactgtga tctacattac ctggcactac cttatgtaaa aagacgatgt
     3061 catcgccccg ttccttaaca gaggtagcca tgtttttcac tgtaggataa acagcagcta
     3121 aatcagtaag ttcatgataa tgggtggcaa aaagagtcaa ggcctttagt ttcgtgcaca
     3181 aatattcaac cacagcacgt gcaatactga tgccatcaaa agtacttgtc cctctcccta
     3241 cttcatctaa aatcaccaga ctattagcag tggcattatt taaaatatta gcaacttcac
     3301 tcatttcaac cataaaagta ctctgtccgc caaccaagtc atcagcggcg ccaatacggg
     3361 taaaaatgcg gtcgactatc ccaatcacag catggcgcgc cggtacaaaa ctgccaagtt
     3421 gggccattaa gacaattaag gcagtttgac gcatataagt tgatttaccg gccatattgg
     3481 ggccagtgat tagcagtact tgctgagtag tgtgttctaa aactgtatca ttgggtacaa
     3541 acaattcgtc ctgcacgact cgttcgacta cagggtgacg gccatcgcgg atttcaatgc
     3601 tgcctgcggc agttatttgc ggccgcacat aacgattctc tctggctacg cgtgccaatg
     3661 actggtaaac atccaattct gccaacacgg cagccgtggt ttgaatctct gtggtgtatt
     3721 gggctacttg gttgcgcagc cccacaaaaa gctcatactc cagagcagat attttttcct
     3781 cagcaccgag gacagtatct tcatattctt taagttctgg agtgatgtaa cgttcagcat
     3841 tggctaaagt ttgtttgcgc gtataatcat caggtaccaa gtgagtgtta gtacgtgtaa
     3901 cttccagata gtaaccaaaa actttgttat aaccaatttt aagcgattta attttcgtcc
     3961 gttcccgctc acgctgctcc atctccacaa tttcagtacg gccatgacta atggcatatc
     4021 gtaatttatc tagttctgga tgaaaaccat cacgcaggag attaccctct ttcagtgcaa
     4081 gtggtggctc gtcagctaag gcggcggtta agcggcttgt taagactgtc agatcgggta
     4141 ttcttgcggc cagagccagc agacaaggcc ccccagtttg taaagtatgt aaaagttctg
     4201 gtatcacctg cagtgttgtg cgcagcgcca ccaaatctct tgccgtagca ctttcacaat
     4261 gaacacggct taacagacgc tctaaatcat atactggcgc tagtaattca ggtaattgtt
     4321 cagccaaagc aaaattatta aggaattctt ccacagcatt caagcgttgt tcaatcgcgt
     4381 ctgcatctaa cagcggttgt tccaaccagc gttttaataa acgcgctcct aacgccgtac
     4441 aagttttgtc gagcacccac agcaatgaac caatcttttg ctcctcacga attgtacgca
     4501 ctaactccag attacgtcgt gttaccgcgt ctaggaccat aaagccgtgt gggtcataaa
     4561 ttactggttt ttgtaaatgg ttgagcttct tttgctgtgt ttcctgcaaa tagacaatag
     4621 ctgccccggc tgccgagacg gcatatggta aatcagcaca acctaaactc tctaaattga
     4681 ccacattaaa ttgggtcagc agcgctccct tggcccgttt tggtgtaaag tcctgatcac
     4741 ggcagggatt gagtattgtc tgattatcaa ggcgtaataa agcttcctgc agtacttcat
     4801 ctgtttgtgc actttcatta ataaggattt ccgctggccg taaacgggcg agctcatcat
     4861 gtaagaattt agctgcctgc tcacctcgtg cttgggctag tctcaactca ccggtggaaa
     4921 tatcaacagc ggcaatacca atgccaccgc gagcttggta aactgcaact aaataattat
     4981 tgcttttttc atccaacaag gaagcatcgg tcaccgtacc tggagtaata acctgaacga
     5041 tttcccgttt gacaatcccc ttggcaagtt ttgggtcttc cacttgttca cagatagcaa
     5101 ctttataacc acgttcaatc aaacgcccta aatatgagtt aagagcatga tagggaaagc
     5161 cggctaaagg cactcctttg tctcttgatg tcagcgtgat ttctagttct cgtgccccaa
     5221 ctttggcatc ttcaaaaaac atctcataaa aatcgccaac ccggaaaagt aaaattttat
     5281 cctgattttt ttctttaatt tctctatatt gacgaatcat cggtgtttct tttttcaagc
     5341 tagttcctcc ccaacaagct ccacgtttta gcttctgtaa ttttcacctc gaccaaagta
     5401 ccgcttgcga tttttccccc tgtaaaatta actgttttgc tgccacgggt tcgccctgtt
     5461 aacacggctg catctgtttt gcttggacct tccaccagca cttcaacctt tttacctact
     5521 agctgctgat tatggtaaag gctatgttta ttttgtaaag tcagcaaacg ctgaaaacgt
     5581 tcttttttta cttcgttggg tatttgaccg ggcatcgcag cagccggtgt accgcttcgt
     5641 ggtgaataaa ggaaagtaaa agcggcatca tagctcactt cctctagtag agctaaagta
     5701 tcgctaaagt cctcttctgt ttcaccggga aagcccacaa ttatatccgt agttatggca
     5761 caattaggca gatagagtct aaacatctct actagctgta tataatgttc ccgcgtgtaa
     5821 cctcgattca tcatttttaa aatgcgatta ctgcctgctt gcaccggcag atgcagatgc
     5881 tcacaaatat tctggccttc cgccatggcg cgggctaatt tttcgttaaa gtcgcgggga
     5941 tgggaagtca taaaacgaat tcgcttcatc cctgtttctt tgtctacacg cgccagtaaa
     6001 tcagcaaaat caattgccgc tgttaaatcc ttaccatatg aattgacgtt ttgacctagc
     6061 aaagttactt ctgaataacc gcgtgttgct agggcagtta cttcttggag aatatcctcc
     6121 ggctttctgc tgcgttctcg cccccgcacg taagggacaa tacaatagga acagaaattg
     6181 ttacagccat agttgatgtt tacccaagct ttgacgccat cctgacgact gacaggcaga
     6241 ccttccacaa catcaccagc ctctgaccat aagtctaaga cggtttcctt acttgctgcg
     6301 gctctttgca aaagttccgg aaaagccgca agattatggg taccaaaaat caaatcgaca
     6361 aaaggaaaat tatccttaat ctgctgagct acctcttcct gctgcaccat gcagccgcaa
     6421 atggcaatca tcagctctgg tcgctctgcc tttaactgtc ctaaacggcc gatacggcct
     6481 aaaaccttcg cttcagcctt ttcccgcaca gcacatgtat tcataataac taaatccgct
     6541 tccctggtat cttctgtttc cacataacct agttcatcca gaaaaccacg cagcgtttct
     6601 gcatctcgtt cattcatttg acaaccaaag actgtcagcg tatatttctg cggcattgcc
     6661 ttcactcctt gcgcatagaa aagattctat ttatcattgt attataaatc aagccaaaag
     6721 caaagggatc gctaacgttg caaataaaaa tactgcaccc atcatgcact tgtgcacaac
     6781 caagcttgat taaacaaaaa gaacccaact tggggttctt tttgtttggt ctatgaaaat
     6841 cgtgttggct aaggtaacat tactaaattt gcttaagagc agcttctctt ttatgatttt
     6901 cagtggcata aaattcagcc tgttcgtccg taagcttgta gccaaatatt gttaagaaaa
     6961 gggcgatcac ggagaacgcc gccggcacaa ggcccataaa cgtcataaat ttactcgcaa
     7021 attccgccgt aatttccata ccggcagtgt aaccgatcaa agccagaccg aagcccaccg
     7081 cagagccacc gactaaaaat ccgattttaa tcggccagtt cataactgtc agcgccatgg
     7141 ttctgttgtc ttgacctgta gcgtagtaac cgtactcgcc gcaatcaagg aaatagttaa
     7201 ccctaaagca agcgttaatg gagatggcgc cttgaatcag gcaggcaaag acgaccatag
     7261 cccaaaggta gttgagccca agaagccaca tgccaagata ggaaatgatt gtaagcactg
     7321 aacctaagat cagcgcgccc ttcttgccaa ctttcttggc gatagatggt gcgacaaggc
     7381 tgaacaaaaa tcccactacg cctcgaattg tcaaaagcag ggccattacc gagaactgcc
     7441 ccgtaaccac tctaaaataa taggccagaa gcggggtata gatctgtgat ccgacagtcg
     7501 ttgctgtcgt caataaaaac atcacgacca tttgtctgtt ggtaataatg gatttgaaca
     7561 tattaccgac agaaggggct ttggcaacct cggcttgctc tctgggtaaa tcgtattttt
     7621 tcataattgg gaaaagcaga aagtagctga gtagcaagaa gacagtacca aatgtaagca
     7681 ccatcacagc atagccgttt gcttcgccaa attgtttacc gaaaaattga atcaagggaa
     7741 ttgttgtcgc ccctgagata atagtaacag cagcagcgat ttgagcctgg cgagcattaa
     7801 ggcgttttct gtcttccatt gaagtacccg ccatcctctg caaaataccg ccctgagatg
     7861 ttgcgcggaa attcatgcca aagtgcaggg tgcagtaaaa gatggagaca ataagtaatc
     7921 tccccaacga tgaagaaatg aaccctgtgg tattgacgag ctgcattaat gaaccaaaaa
     7981 ataacgtgta agtggtcagt ctcatccata aagcgtactt gccgtgtttc ttgctgtgag
     8041 ttttctcaat aatgctgccg gcaaagatgg atacgacata gtcgagcaat tttgcgacca
     8101 acattgctgt gcccatagcg gcagcaggaa tacctagata ttcggtcata aacaacattc
     8161 cgtactgagt cgatggttgg tgcaccatgg cggaaacagc cccaggcaga gcatacgcca
     8221 ttatttcact gtttttaatt ttcgattttt ctttttccat tttaatcccc tttgcatgat
     8281 aagaagatag ctcacgttcg taactacctt cttatgtatt ttttgctgcc gatcaacaaa
     8341 ctgcttacta gtcgcctgaa ctcacacaac tcgccattat acaacattat aagttacatt
     8401 ttcgttatag tcaataactt tgcaattatc tgaatagtaa aagaagaaat taactaaaat
     8461 aaaataaaat gactaggggc tgattcctgt cagcccctag tcatttttcc tccaattccg
     8521 taaagggaac cttcatcgtg cgacatgttc ccatcactga tacattggcg gtagtttctt
     8581 ccacaccttc tatccacact ggtttgccct gaaaatgtac ttcgattttt gcgggcgagt
     8641 ttaggagctc ctcagctcgc tctatgtcca ttttatcaac tccttcaggt agctgtattt
     8701 agtctatatt cacctttagt atgagttggc aagtggactg attatgtatc tttatatttt
     8761 tataatatca catatttctg cttgattagc gtcgagtaaa acttgcggtg ctgctttaac
     8821 caggctgttt tccgaaccgc ccccagtgta tacttcagtc agtgtaaaga tgcgcttatc
     8881 cattaaaaat atcgctttaa aaccaaagga aggtgtaccg ccacaaggaa agtttgtccg
     8941 ctctaaaatt tcgtcagctg tagccatacg catgcttttt atgcctaaca cttgcgctgc
     9001 ctttttacgg tcaacacgat ctttgccgcg aacaatagca acaactaatt tttcgtctga
     9061 accaattaga caaatattct tgacaaactc atcactggaa gcaccgactg cagctgcagc
     9121 ttctgctaca gaatgacagg attcagtaaa acataagtgt tcggcagcta gctgctgctc
     9181 ttgtatataa gcctgtaatt tacgcgcata gggcattgaa ctatctctcc ttgataatct
     9241 cgcctaaggc caaaatgatt tctgtaactt cttctactgt attttgatgg ccgaaagaaa
     9301 agcgcagcgt ccccaccgga aaactaccga tagtttggtg agccagtggt gcacagtgga
     9361 ggccagagcg agtcattatg ccatagccct gttccaacag atagcttaag tcgccattat
     9421 ctactccttg taagtttaaa gaaacagtag cagtcgttgt cgacgggtca ttagtaccat
     9481 aaacctgaat ttgaggcagg ccagccagtc cgtataaaaa agctgcagtt aattcttctt
     9541 tctgttgctt gattgatgtt aaaccaattt gctgtatagt tttaactccc gctgccagtc
     9601 ccgcaatgcc cggagcattt tgtgtaccac tttcaaattt atccggcaag aattccggtt
     9661 gttgttcctc ctctgaacga ctccctgtac cgccctccac gagaggctta gttaaggcag
     9721 cggccctttc acttaagcag agtccaccga tacctggcgg gccaagtaaa cctttatgac
     9781 ctgtaaatgc tagatagtca cactgcagct cagcaaaatt gatttcttct gtgccagctg
     9841 attgagcagc gtcaacacag tacaaagtat ttgtacctgc caatatttcg ccaatctcat
     9901 aaacaggcat aatggtacct gtgacattag aggcgtgtgt taagatcacc agacgtgtat
     9961 ttttgcggag cgcacgcttt atttggtttg gttctaaggt gccgtctgct tgacatgcta
    10021 ttcgttccac agaaattttt ctttcagcgg ctaaacgggt taaggggcgg acaacagcat
    10081 tatgttccat agaggatata agcacatggt cgcctgctgt caacaaacct ttaatcacta
    10141 aattaagcgc ataggtaaca ctaggtgtaa aaattatctg ctccggttgg ggaacattaa
    10201 aaaaatcagc caagatggtt cgtgtttcgt aaaccagacg tgcagcgttt aaggcccgac
    10261 gataaccacc gcgacccggg ctgcagccaa tatcacgtat gtagcttacc atagcttcag
    10321 ctacttgtgg tggtcgggga aaagttgtgg cagcatgatc agcatatatt tctttcatct
    10381 ttttctggcc ccctctgaaa gaatgacaat acaaaatctc taaaagcaat cgctgcaggg
    10441 gtgaggatgc gtttggcatg agcaacaagg taaaaatctc gcgttaactg taaatcctgt
    10501 aagtggcgcg tcgccactaa gccaaactgc agataatcct ccactacata acgagaaatt
    10561 atggtgacac ccaaaccatg gcggactgct tgtttgattg tttcttggtt atcaaaaatt
    10621 tgcagctgta agtctgctaa gtccatccca ttgcactgca gctccctggt aaaaagagaa
    10681 cgtgtaccgg aacctgcttc acgcagcagc aaattatagt tcaacaaatc cgccattgtc
    10741 aacgtttctt tcattaacag ctcttcataa gcagcttgtt gaggaatggc aacgactaat
    10801 tggtcagata ctaagggttc ataaactaaa tgagggatag ataattgctc acccactatg
    10861 cctagcgcat aatgataatg aaagacatct tcactagctt tgcgggaatt ttcttggcgc
    10921 agcgtcacct taacctgcgg ataaagctgc tgaaaatcaa ctattgcttt gggcagcaga
    10981 tattgacagg gtacagaact ggcggcaaca atcaattttt cttcctgtat tacccccgcg
    11041 cctaattcag ttagggcatc agtgcgtaat tgcagtaaat ccttagcata acgaaaaagt
    11101 gttgtccctg ccggggttaa gagtaagccg tttttccccc gatcgaagag cggcgtgtta
    11161 agctctgttt ctaaactctt gacgtggctg ctaatcgttg gttgacttaa gtagagctgc
    11221 tcagctgcac gggaaaaaga gcgatgtgca gcaaccatga caaaggcctc taactgataa
    11281 aattccatta gcccactctc cttccgctat attattgttc ggaattatca aggacaactc
    11341 ctgccaaata acataaactt taacttcttt ttcatttatg ccattggatt tatct
//
LOCUS       DASRMS010000096         1466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_425960, whole genome shotgun sequence.
ACCESSION   DASRMS010000096 DASRMS010000000
VERSION     DASRMS010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1466
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425960"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1401)
                     /locus_tag="VFC74_04380"
     CDS             complement(<1..1401)
                     /locus_tag="VFC74_04380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24605.1"
                     /translation="MIKLFIKFSALTCALLSAVAYADSTEVWVDSYTPFEAIEAQPAA
                     PPTNPITHYAKEVPVITLEPFIESKKISDSRAFKANNPRTLSAPLNIGVKRVVSETDT
                     VEKVDSYLAWETLEDNSHIAALSIISPHALGNRLVLNIEKLDPRAELRFSNANNDEVF
                     TVSGEDIINTIFKDKQTNEDQETNKNLYIGPYLSGEQATLEIFLPKKVAIDSVELSLP
                     YLSHIFLSPTDLELLNQGSNDLSCMVNVACDSSWNNANGIARMLYTNTLGSTYVCTGT
                     LLADRLNTREPYFLTSSGCISSQELASNLQTFWLYKTDSCYANTLNNSYKRLTGGADL
                     LFKHDSTKTALLRLHDQAPAGVTFQGWSSEDAKNRYVSVVQYPAVAPQKVAKGYSGVS
                     LADCQYAASGTLSCRDSHSHDAGFLKVEYNSGVTADGSNGSGMFITEKNNQGKKKHYL
                     VGQLFTGSSSCTAPSAP"
BASE COUNT          358 a          294 c          352 g          462 t
ORIGIN      
        1 tggggctgag ggcgctgtac agctagagct gcctgtgaac agctgtccta cgaggtagtg
       61 tttcttttta ccctgattgt tcttttctgt gatgaacatt ccagagccgt tgctgccgtc
      121 tgctgtcaca ccgctattat actcaacctt tagaaaccca gcgtcatgac tatgggagtc
      181 tcggcagctt aaagtgccgc ttgcagcata ttgacagtca gcgagggaga cgccgctata
      241 ccctttagct actttttgtg gtgcgacagc agggtattgg acaacggaca catatctgtt
      301 ttttgcgtct tctgaggacc agccttggaa ggttacgccc gcgggtgctt gatcatgcaa
      361 gcgcagtaag gcggttttcg tactgtcatg tttaaatagt aagtcggcac ctccagtgag
      421 ccttttgtaa ctgttgttta gtgtgtttgc gtaacaggag tctgttttat acagccaaaa
      481 agtttgcagg ttagaggcta attcttgaga gcttatgcaa ccagagctgg ttaggaagta
      541 aggctctctg gtatttaatc gatcggcgag aagtgtaccg gtgcatacat aggtcgatcc
      601 gagtgtattg gtatacaaca tcctggcaat gccattggcg ttattccagc ttgagtcgca
      661 cgcaacattg accatgcagc ttagatcgtt actgccttgg ttgagtaact caagatctgt
      721 cggtgataga aaaatatgcg ataagtacgg caacgacagc tctacactat ctatggctac
      781 tttttttggt aaaaatattt ctaatgttgc ctgttcaccg ctgagataag ggcctatata
      841 caggttctta tttgtctctt gatcttcgtt tgtttgtttg tctttgaaaa tggtgttgat
      901 aatgtcctca ccagagacag tgaatacctc atcgttatta gcattgctga agcgtagttc
      961 ggctctagga tctagctttt caatatttaa gacaagacga ttgcctaaag catgtgggga
     1021 aataatactt aatgcagcga tgtggctgtt atcttccaga gtttcccatg ctaaatagga
     1081 atcgactttt tctaccgtgt ctgtttcaga aactacccgt ttaaccccaa tgtttaaagg
     1141 tgcgctaagt gtcctggggt tgtttgcttt aaaagcacga gaatctgata ttttttttga
     1201 ctcaatgaat ggctccagcg ttatgactgg aacctctttt gcataatgcg tgattgggtt
     1261 ggtgggtggg gctgctggtt gtgcttctat tgcttcaaac ggggtataag aatcaaccca
     1321 aacctcggtt gagtcagcat atgccactgc agagagtaga gcacacgtca gtgctgagaa
     1381 ttttataaat agcttaatca tgagacttta atctccatat ttaaagcttt agcagtcact
     1441 actttttcga ggaatgttat gcataa
//
LOCUS       DASRMS010000097        17536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_156955, whole genome shotgun sequence.
ACCESSION   DASRMS010000097 DASRMS010000000
VERSION     DASRMS010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 17536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17536
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156955"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /gene="hisG"
                     /locus_tag="VFC74_04385"
     CDS             <1..345
                     /gene="hisG"
                     /locus_tag="VFC74_04385"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZK24606.1"
                     /translation="AVARRVGSGNFTGGYVATKYPRITERYFQQQGKQFELIKLHGSI
                     ELAPLLNLAEVIVDLVSTGKTLAENHLEEVETITHITSRLIANPGSYQVKGERVMALT
                     KALAQVLSQKEV"
     gene            348..1673
                     /gene="hisD"
                     /locus_tag="VFC74_04390"
     CDS             348..1673
                     /gene="hisD"
                     /locus_tag="VFC74_04390"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010240649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HZK24607.1"
                     /translation="MLKEFDGATFLQQFKRRGFDDYPTELAAVRQILTAVRQRGDAAV
                     LAYTERFDQAKLAALEVTPAEFKAAQTSVEPQIKADLQQAADNIADFHRRQIQQAWVD
                     YGADGIILGQRITAIDRVGAYVPGGAAAYPSSVLMTVIPARIAGVGEVIMVTPPQADG
                     SINPYTLVAAQIAGVDAVYKCGGAQAIGALAYGTASIRRVDKIVGPGNLYVTLAKREV
                     AGVVGIDMLAGPSEVLVVADETARTEFVAADLLAQAEHDPLAAAYCVTTSVRLAAELP
                     AELQRQCAALDRQAVAQQSLTTQGALVTVATLAEALEIVNLLAPEHLELQIADPWAAL
                     GQIRHAGAIFLGAYTPEPLGDYWAGPNHVLPTSGAGRFSSVLSVDDFIKKTSVLAYSP
                     AALLKCSAAVENIAAVEGLTAHSSAVARRRKLLEQENGKCSSSDERNRD"
     gene            1633..2211
                     /gene="hisB"
                     /locus_tag="VFC74_04395"
     CDS             1633..2211
                     /gene="hisB"
                     /locus_tag="VFC74_04395"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HZK24608.1"
                     /translation="MASVRRQTKETEIDLTLVLDGKGEAELDCGVPFLEHMLTLWSYH
                     ACFDLQLQAKGDWQVDAHHVVEDVGLVLGETLHQALGDKAGITRYASLALPMDEALVL
                     VAIDLSGRPYLGFDLPLPAERVGNFETELVEDFLRAFTNRAHCTLHVKLLAGRNTHHI
                     IEALFKGLGRVLGTASRVEGRVPGIPSTKGLL"
     gene            2318..3046
                     /gene="hisA"
                     /locus_tag="VFC74_04400"
     CDS             2318..3046
                     /gene="hisA"
                     /locus_tag="VFC74_04400"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003949 -
                     1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HZK24609.1"
                     /translation="MLIIPAVDIRDGRCVRLVHGELQRETMYYENPVEAALHWESEGA
                     QRLHVVDLDGAFDGSIKNIAVIEEIVRAVQIPIQVGGGIRESQVAADLLARGVSRVIM
                     GTAAVQQPQVVAELCARYPGQIMVGIDARAGRVAIRGWVEEAPMLAIDLAQQMAQLGV
                     REIIYTDILRDGTLKGPNVAALAEVTQAVSLSVIASGGIASLQDILELTPLQPQGLSG
                     IIVGQALYTGRISLPAALAITEGL"
     gene            3047..3826
                     /gene="hisF"
                     /locus_tag="VFC74_04405"
     CDS             3047..3826
                     /gene="hisF"
                     /locus_tag="VFC74_04405"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012284027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HZK24610.1"
                     /translation="MLTKRIIACLDVKDGRVVKGTQFINFRDAGDPVALAKFYDQAGA
                     DELVLLDITASHEGRSTMLELVERTAAEVHIPFTVGGGISELKQMQQIISAGADKISL
                     NTAAVQQPALLTKAAAQFGSHRLVLAVDAKWSEALQTWEVYTHGGHRATGKAVLSWVA
                     EAEQRGAGSILLTSMDCDGAKGGYDLPLTAAVAEVAAIPVIASGGAGSKEDFYTVLTE
                     GKAAAALGASVFHYQQFSIAEVKEYLAARGVPVERERNTEI"
     gene            3804..4454
                     /gene="hisIE"
                     /locus_tag="VFC74_04410"
     CDS             3804..4454
                     /gene="hisIE"
                     /locus_tag="VFC74_04410"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HZK24611.1"
                     /translation="MNVTLKFNQQGLIPAIVQDIQTGSVLMLAYMNEASLQKTLASGQ
                     TWFYSRSRQELWHKGASSGHTQTVKRIDYDCDGDALLVYVEQSGGACHTGAATCFYRT
                     LHGEKTAAPELFLPELERIIAERQVNRPADSYVAQLFNGGLDRILKKVGEEAGEVIIA
                     AKNEERSELIYESGDLLFHLLVLLAAKDVGFSEVLAELQRRHQPAAKEQGAKHHES"
     gene            4444..5253
                     /locus_tag="VFC74_04415"
     CDS             4444..5253
                     /locus_tag="VFC74_04415"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase HisJ family protein"
                     /protein_id="HZK24612.1"
                     /translation="MKVDYHLHTARCGHATGTMAEYLAVAEAHGVTEVGFADHFPLEL
                     LGYVPQDPVNMPPAELPAYIAEVQYWQKEAPLAVRLGAEVDYLPGREKLTAAALAAFD
                     FDYLIGSVHFLADWDFTHPAYQERFQEVNMDRLYAEYFALIQQLAQSGLFQIVGHLDV
                     LKKFAYFPSSDWSMLVEETCQILKATDMCVEVNTAGWRAPVREVYPGVSLLRKCQQLG
                     IPVTLGSDAHQPQDVGRDLERALLLLEKLGFREITTFARRQRKLQPLVISS"
     gene            5312..5767
                     /locus_tag="VFC74_04420"
     CDS             5312..5767
                     /locus_tag="VFC74_04420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009616410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HZK24613.1"
                     /translation="MKLSTKGRYGVRAMFDLALHSGQGAIALKHVAQREQISEKYLEH
                     LFANLKKAGLIYSVRGAQGGYRLARPAEEITLGDIIRVLEGPVAPTDCVIKGAAGKND
                     KCNRATACVMHHVWSRLSDEINDLLDSVTLAEIVEEQRRLTGEGYMYYI"
     gene            5887..6396
                     /locus_tag="VFC74_04425"
     CDS             5887..6396
                     /locus_tag="VFC74_04425"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HZK24614.1"
                     /translation="MNKILDLTGSVTTAAGEMTVSYTAAGLYALELPGFAAGSYLPVS
                     SSEPAWLQKLALDLQNYFLGQTVHFTCPWDEANYPPFYQRVLQAAAQIPYGECRTYKW
                     LAQQAGSPGAVRAAGQAMAHNRTPLVIPCHRVLRSDGKLGGFAYGLSWKERLLTLEKA
                     AREDLNNAN"
     gene            6386..7141
                     /gene="lgt"
                     /locus_tag="VFC74_04430"
     CDS             6386..7141
                     /gene="lgt"
                     /locus_tag="VFC74_04430"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZK24615.1"
                     /translation="MQIDPIAFRLFGIPIYWYGVMISSALLFGTLVAMRRAPQYGVDE
                     DDLLGFLLLAVPLAIVGARAVFVVENWDTMAGDFLAMINLRQGGLSIHGALIGGIIAG
                     LFYTQFKRLSFWSIADITAPGLILGQAIGRWGNYFNQEAYGIPTDLPWALYIDGAYRH
                     PIFFYEFVWNLLVFAYLIRISKKEKTPGGIFLRYLVGYSIGRFFIEGIRANTVFWGNL
                     RLGQIVSVLLIVIGLLLLWWRKRQTKDEGGIVR"
     gene            7141..8304
                     /gene="aroC"
                     /locus_tag="VFC74_04435"
     CDS             7141..8304
                     /gene="aroC"
                     /locus_tag="VFC74_04435"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HZK24616.1"
                     /translation="MRYLTAGESHGPAITTIIEGLPANLPLDQLEINQDLTRRQQGYG
                     RGGRMKIEKDQVEILAGLRFGKTLGSPLTLQVINKDYAQWSDKMAPFGEQPPEIALVT
                     HPRPGHADLPGALKYNLADIRNVLERASARETVARVAVGAVARSLLRAFDIQIYSYVE
                     AIGQVSAAAQKVAALVAANYAGVENSPVRCPDPTAAQQMTKAIDEAKAAGDSLGGIFV
                     VAALGVPVGLGSHVQWDRKLDARLAAALMSIQAIKGVEIGEGFSLAAQPGSEAHDEIY
                     YHHSKGYYRQTNHAGGIEGGMSNGEAIVCRVAMKPIPTLSKPLQSVNMQDHRPYTAAV
                     ERADSCAVPAAAIVGEAVMAWELAQALREKLGSDSLEEMQDNYRTYLKTIAKR"
     gene            8317..9402
                     /gene="aroB"
                     /locus_tag="VFC74_04440"
     CDS             8317..9402
                     /gene="aroB"
                     /locus_tag="VFC74_04440"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HZK24617.1"
                     /translation="MKTVQVSLAERSYPIFIGKDLWSELPQQLPLALAPASALLVSDT
                     NVYPLYGDKISAAFTAAGFKVTPAVVPAGEGSKTLARAEELYTASIKAGLDRASVIVA
                     LGGGVVGDLAGFIAATYLRGVPFVQIPTSLLAQVDSSVGGKVAVNHPLGKNLIGAFYQ
                     PKLVWIELEVLATLPQREFLAGAAEVVKYGAILSEPLWTKLEQSWPDFLAQEQAVLTQ
                     VIIDCCTLKAEVVAADERETGWRTLLNFGHTLGHALEAATRYTYYLHGEAVLVGMTLA
                     VQLALQQQILAPAAGVRLLALFRRLGIKPAPPTLTAAPVLAALQQDKKRVGKKLPFIF
                     PLQIGQASTFDSIPPVLLSSMLQEYLN"
     gene            9547..11682
                     /gene="secD"
                     /locus_tag="VFC74_04445"
     CDS             9547..11682
                     /gene="secD"
                     /locus_tag="VFC74_04445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HZK24618.1"
                     /translation="MRRNRLLILILIIGLLLVGSAAAIWQAANSLNLGLDLRGGVYVL
                     YRAAETEGAADDADKLARAITIIRTRVDALGVAEPNIQKEGQDRIRVELPGIEDQRVA
                     REVIGKTALLKFVGPDGETIVSGDELKDAFVSYDQYNRIAVSLEFNAVGSEKFAQATE
                     KLLGQQINIYLDEDLISDPGVNTVIPDGKAQITGNFTAEEASNLALQLRSGALPVKLE
                     ELEIRGVGPQLGRDSLARSVRAGIAGIILVLLFMLVYYRALGLMAGVSLVVYAAIVLG
                     VMSALKVTLTLPGIAGLILSVGMAVDANVIIFERIKEELSNGRTMRPAVEAGFTRAFR
                     SIFDSNVTTLIAAVVLFQFAQGSVRGFAVTLSVGILVSMFTALVLTRFLLRQAAGTAL
                     LPTPQHGLQTETSTTKRGPNYIGLRKAALIFSILLILAGMTSLATRGLNYGVDFTGGT
                     LLQIDLGQEFTLDEVRDTLAPFDLEAATVQKVGSETLAGNESEQKSQEVLIRTPELTA
                     AQQDEVFEAFQERFAIAEDALLRVENVGAAVGDELQRQAVIALLLASLGMIIYITIRF
                     EYRFAITAIIALLHDAAILLTFFALFRQELNGTFIAASLTILGYSINDTIVIYDRIRE
                     NMKYQRKESLVEIVNNSIAQSLRRTINTSLTTLLVLVTLLIFGGVTLRPFISALVVGV
                     VFGTYSSIFIASPLWLWWKEREAKKKALT"
     gene            11868..12296
                     /locus_tag="VFC74_04450"
     CDS             11868..12296
                     /locus_tag="VFC74_04450"
                     /inference="COORDINATES: protein motif:HMM:NF018058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HZK24619.1"
                     /translation="MQLGIIVGLLFGVLIALFAFVNRATVVVNYFFGQVEASVALIIL
                     ASAMSGALAVGLFGLITQIRTGFTIWNYKKKTKSFAKEVEELKTQKQALLDDLAALHA
                     ESELLLQQEKQSLEQQQNNVETAAEIAAVEAAEETSNDSE"
     gene            12283..14652
                     /gene="recJ"
                     /locus_tag="VFC74_04455"
     CDS             12283..14652
                     /gene="recJ"
                     /locus_tag="VFC74_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HZK24620.1"
                     /translation="MTVNKWAWAVSKVDQQAVHQLTQELDIHRLTATTLYQRGLHSPQ
                     EARAFLSCDLSDLGDPFSMAGAAAAAVRIAAAIEQNEKILIFGDYDADGVTATALLVR
                     ALQNLSADVAYYIPSRFAEGYGLQAAVLEEYAAAGVQLVITVDCGINSFAEMELAKQR
                     GFDLIITDHHTCFPGTRTAMAVLNPKQTTCSYPEKQLAGVGVAWNLVRALYATLQLPV
                     EATYDYLDLVAVGTIADVVPLIGENRLLVKQGLARLSANPLPGLAALARVSDLTDTKL
                     TATQVAFTLAPRLNAAGRLGKADPAVETLLAAPAEADAWARELEAMNRQRQQIEKVIT
                     AEARVLAEAQTSAPALVLWQEGWHAGVIGIAAGRLAREFKRPVVLIALEGAEGRGSIR
                     SIPGCNVVTALQDCASHLLKYGGHAEAAGLSIAAAELEAFRQAFCQAVRDQACTEPIL
                     PVVAETNFSELTLALVKELEILAPFGQGNREPVFLVNGAEVINTQRVGAQRDHLRLTL
                     RQEAVVQKAIYFGGGEQQLNPRERVELIVMTSLNHWQGRTLLSLQVKDLRPAAADQHR
                     TPTIIDQRNRKLSEQRLARLAAEKNLLIWVNTKAAQTGLEKTLARGAAVTQLGRKVDY
                     ARTYDALLFYHLPYQRKACEQLLQNLSFNDAPQVYLCYGTEEEALNEKIFAATVPAAE
                     TLQQLASCVSPAEPNLTAGKVEHKLKQPVTQYLIKEAQAVFTEIAAGQNTTWPPQESQ
                     LQQSPTYRHYDRVLTRFRAYQNFWRQATAAEITTYLLNPEQLTLEEEEI"
     gene            14657..16816
                     /locus_tag="VFC74_04460"
     CDS             14657..16816
                     /locus_tag="VFC74_04460"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013361518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HZK24621.1"
                     /translation="MSLQALKEIIKGYYPVEPNWDLLEKGYNFAVNAHQGQKRFSGER
                     YIIHPLGVSIILAELELDLDTIVAGLLHDVVEDTEVTLEDIETEFGADISLLVDGVTK
                     LSKLEFKSKEERQAENLRKMFMAMAKDIRVLLIKLADRTHNMRTLKYLTSIKQQAISR
                     ETLEIYAPLAHRLGIYKIKWELEDLAFRYLERDRYYALVDRLAKKRQEREAFITQIMG
                     ALQPKLQDVNINAQISGRPKHLYSISQKMKEQNKEFHEIYDLTAIRIIVNSLKDCYGA
                     LGIVHTLWKPIPGRFKDYIAMPKSNMYQSLHTLVMVGKNELLEVQIRTWEMHRTAEYG
                     IAAHWRYKEKNDSKQEEEFEQKLTWLRQIMELQQDSKDAAEFMENVKIDLFADEVFVF
                     TPKGDVIDLPAGSIPLDYAYKIHTDIGHRCIGARVNGRLVTLDYELKTGDIVEIMTSK
                     QGSPSRDWINMVKSSGAKAKIRAWFKKERRDENLAKGKELLEKELRKQELDPHLYLKT
                     ALLQEMAKKFNIHSEDDAYVAIGLGGVTAQQAVSRLKEEYRKKYGPPEPEPEKEFIPQ
                     KTVSKTGKGVVIAGIDSMLLRFAKCCTPVPGDQIVGVVTRGRGVSIHRQDCLNVLNHP
                     DQHRLLEAHWEENAEGAYPVEIEITGMDRPQILKDVVTAVSESKVNINALTGRGTKDR
                     MSIIQMTLVVRDLDHLENVINRLSRVRDVYDVHRIHT"
     gene            16827..17279
                     /gene="dtd"
                     /locus_tag="VFC74_04465"
     CDS             16827..17279
                     /gene="dtd"
                     /locus_tag="VFC74_04465"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HZK24622.1"
                     /translation="MRAVIQRVAEARVEVDGAIIGQINKGLLILLGIQAADQEADLRY
                     LVGKIAHLRIFEDEAGKMNLSLLDIGGSALVVSQFTLYADTRRGRRPGFSYAAGPDQA
                     EPLYEQFCNVLASFGIPVATGRFGATMDVQLTNQGPVTIIIDSSDKTS"
BASE COUNT         4444 a         3912 c         4642 g         4538 t
ORIGIN      
        1 gcagttgccc gccgggtcgg cagcggcaac tttacaggag ggtatgtggc gactaaatac
       61 ccccgaatta cagagcgtta ttttcaacaa cagggtaagc agtttgagct gattaagctt
      121 cacgggtcta ttgaactggc accattgcta aatttagctg aagtaattgt agatttagtt
      181 tccaccggca agactttagc cgaaaatcac ttggaagaag tagaaactat tacccatatt
      241 acctcacgtt tgattgccaa ccccggcagt taccaagtta aaggggagcg agtgatggca
      301 cttaccaagg ctttggcgca agtgctgtcg cagaaagagg tgtaaagatg ctaaaagagt
      361 ttgatggagc aacctttttg cagcaattta agcgccgcgg ctttgatgat taccccacgg
      421 agttagcagc agtgcggcaa attctcacag cagtgcggca gagaggcgat gcggcagttt
      481 tagcgtatac ggaacgtttt gaccaggcca aacttgcggc cctggaagtc acgcctgcag
      541 aattcaaggc agcacaaacg agcgtggagc cgcaaattaa agcagatttg cagcaggcgg
      601 cagataatat tgctgatttt catcgccggc aaattcagca agcctgggta gactatggtg
      661 ctgatggcat tattttaggg cagcgtatca cggccataga tcgcgttggt gcttatgtgc
      721 ctggtggtgc tgctgcttat ccttcctcgg tactgatgac cgtcatcccc gcacgtattg
      781 cgggtgtagg cgaagtgatc atggtgactc cgcctcaggc agatggcagc atcaaccctt
      841 ataccttggt cgcggcccaa atagccggcg ttgatgctgt ttacaaatgc ggcggggctc
      901 aggctattgg cgctctggct tatggtaccg caagcatccg gcgggttgat aaaattgtcg
      961 gccccggcaa tctgtatgtt acactcgcta aacgggaagt ggcaggggta gtggggattg
     1021 atatgctggc gggtcctagt gaagtgctgg tagtcgcaga tgagacggcg cgtactgaat
     1081 ttgtggctgc ggatctactt gctcaggctg aacatgatcc tttggcggcc gcctactgtg
     1141 tgaccacctc cgtgcggctg gcggcagaat tgccggcgga actgcagcgt cagtgtgctg
     1201 cactggatcg tcaggcagtg gcccaacaat ctctcaccac ccaaggggcc ttggtgaccg
     1261 tagcaaccct ggctgaagca ctggaaattg ttaatctgct ggccccagag catttagaat
     1321 tacaaatcgc tgacccctgg gctgctttag gtcaaattcg tcatgctggt gccatctttt
     1381 taggggcgta tacgccggag ccgctgggtg actactgggc agggcctaat catgtgctgc
     1441 ccacgtctgg tgctggtcgt ttttcttcag ttttatctgt agatgacttt attaaaaaga
     1501 ctagtgtcct tgcttattca ccagcagcac tgctaaagtg ttctgcagca gtggaaaaca
     1561 tcgcggcagt ggaagggctg actgcccaca gcagtgccgt tgccaggagg aggaagttac
     1621 ttgaacaaga gaatggcaag tgttcgtcgt cagacgaaag aaacagagat tgatttgact
     1681 ttggtactcg acggtaaggg agaagcagag cttgactgtg gggtaccatt tctagagcat
     1741 atgctgactt tatggagcta ccatgcttgc tttgacttgc agctgcaggc caaaggagac
     1801 tggcaagtgg acgcacatca tgtcgttgaa gatgtcggct tggtgttggg tgaaactctg
     1861 caccaagcac tgggtgataa agcaggcatt actcgctacg cctcgttagc attacccatg
     1921 gatgaagcgc tggtactagt ggcgatagat ttatcaggcc gcccttatct aggttttgac
     1981 ctcccattac ctgcggaacg tgtcggcaac tttgaaacag agctggtgga agattttttg
     2041 cgtgctttta ctaatcgggc gcactgtacc cttcatgtta aacttttagc cggccgcaat
     2101 acgcatcata ttattgaagc gctttttaag gggttaggac gagtactagg cacagcgtcc
     2161 cgagtagaag ggcgagtgcc agggattcct tccaccaaag gattattata aatcctaggg
     2221 atttagcagg atttttttct agtatcaggg aatatactag gaagaaatct ggggaacctt
     2281 cgcttgacta caatatttta aatgggaggt tggttgcatg ttgatcatcc ccgcagtaga
     2341 tatccgcgat ggccgttgtg tgcggttagt acatggtgaa ctgcaacggg agacgatgta
     2401 ttatgaaaac ccggtggaag cagcactgca ttgggagtca gaaggtgctc aacgcttaca
     2461 tgtggtagat ttagacggtg cctttgacgg ctcaataaaa aatatagcag tgatcgaaga
     2521 gattgtgcgt gctgtccaga ttcctatcca agtggggggc ggcatccgtg agtcgcaagt
     2581 ggcagctgat ctgctggccc gtggtgtatc tcgtgttatc atgggtacgg cagcagtgca
     2641 gcaaccgcaa gtagtggcag agctttgtgc acgctatccg ggacagatta tggttggtat
     2701 tgatgctcgt gctgggcggg tagcaattcg tggctgggtg gaagaggccc ccatgctggc
     2761 gatcgacttg gcacaacaaa tggcgcagct gggtgtgcga gaaatcatat atacagatat
     2821 tttgcgggat ggtaccttaa aaggtcccaa tgttgcagca ctggcggaag tgacacaagc
     2881 ggtgtccctt tcggttattg cttctggcgg cattgcttct ttacaggaca ttcttgaact
     2941 tacgcctttg cagccacaag ggctgagcgg cattatcgtc ggacaagcac tgtacacagg
     3001 acgcatctct ttacctgctg ctctagccat tacggaggga ctgtaaatgt taacaaaacg
     3061 tattattgct tgtcttgatg ttaaagacgg tcgcgtggtt aaaggtacgc aatttataaa
     3121 ttttcgtgat gctggagatc cagtggcact agccaaattt tatgaccaag ctggtgcaga
     3181 cgaactggtc ttgttagata ttacggcttc tcatgaggga cgcagcacga tgcttgagct
     3241 agtggagcgt acagctgcag aagtacatat tcccttcact gtaggcggcg gtattagtga
     3301 gctgaagcaa atgcagcaaa ttatctcggc aggggctgat aaaatatcgc ttaatactgc
     3361 cgctgtacag cagccagcac tattgaccaa agcggcagca caatttggca gtcaccgtct
     3421 ggttttagcg gtggatgcca aatggagtga agctttacaa acgtgggaag tgtatacgca
     3481 cggtggtcat cgggcgacgg gtaaggctgt cttgtcatgg gtagcagaag ctgaacagcg
     3541 tggtgccggc tcgattcttt tgaccagtat ggattgtgat ggggctaaag gtggctatga
     3601 tctgccttta actgccgctg tcgccgaggt ggcagcgata ccggtgattg cttccggtgg
     3661 tgccggcagt aaggaagact tttatactgt cctgaccgag gggaaggctg cagcggcctt
     3721 aggcgcctcc gttttccact atcagcagtt tagtattgcc gaagtaaaag aatatttggc
     3781 tgcacggggg gtgccagtcg agcgtgaacg taacactgaa atttaatcaa cagggcttaa
     3841 tccctgccat cgtgcaggat atccagacag gtagtgtttt aatgctagca tatatgaacg
     3901 aagcatcttt acaaaaaaca ctggcgagcg ggcaaacttg gttttatagc cgcagccgtc
     3961 aggaactatg gcataaaggc gctagttctg gtcatacgca aactgttaaa cgcattgatt
     4021 atgattgtga cggtgatgcc ctgctggtgt atgtggaaca aagtggtggt gcctgtcata
     4081 ccggtgccgc tacctgtttt tatcggacac tacacggtga aaaaacagct gcaccagagc
     4141 tgtttttgcc tgaactggag cgcattattg ccgagcgtca agttaaccgc ccggccgact
     4201 cctatgtggc gcagcttttt aatggtggcc tggatcgcat tttaaaaaaa gtcggtgaag
     4261 aagcaggaga agtgattatt gctgcaaaaa atgaagaacg cagtgagtta atttatgaaa
     4321 gtggcgattt gctctttcac ttactagtat tactggcagc caaagatgtg ggattctcgg
     4381 aagtgttggc tgaactacaa cgtcgacatc agcctgcagc aaaagaacaa ggtgcaaagc
     4441 atcatgaaag ttgattacca tctccatacg gcacgctgtg gtcatgctac tgggacaatg
     4501 gcggaatatc tggctgtcgc cgaagcacac ggggtgacag aagttggctt tgcggatcac
     4561 tttcccttgg agttattagg ttatgtgcca caggatcctg ttaatatgcc tcctgcagaa
     4621 ttacccgcct acatagctga agtgcagtat tggcaaaaag aagcaccgct agccgtgagg
     4681 ctgggcgcgg aagtggatta tctgccgggc agggaaaaat taacggctgc tgctttagcc
     4741 gcctttgatt ttgactattt gattggctca gtgcactttt tagcagactg ggattttacc
     4801 catccagctt accaagaacg ctttcaagaa gttaacatgg accgacttta tgcagaatat
     4861 tttgctctca tacagcagtt agcgcagagc gggctttttc agatcgtcgg ccatctagat
     4921 gtgcttaaaa agttcgccta tttccccagc agcgattggt caatgctggt agaagagact
     4981 tgtcaaatct tgaaagcaac tgatatgtgt gtagaagtta atactgcagg ttggcgagca
     5041 cctgtgagag aagtctaccc aggtgtttct ctcctgcgaa aatgtcagca gctgggtatt
     5101 cctgtcacct tgggttcgga tgcccaccag ccgcaggatg tagggcgaga cctggaacgc
     5161 gctctgctgc tgctagagaa attgggcttt cgtgagatta ccacttttgc cagacgacag
     5221 cgcaagctgc agccacttgt gatcagctct taatgaactg cagttgatgt gataaaatca
     5281 tgatgatact agtaaaggca ggagattatt aatgaaattg tccactaaag gtcgctacgg
     5341 tgtacgcgct atgtttgatt tagcgctgca tagcggccaa ggtgcgatag ccttaaaaca
     5401 cgtggcccaa cgggaacaaa tttcggaaaa atatttagaa catctttttg ccaatctcaa
     5461 aaaagcaggt ctgatttaca gtgtgcgtgg ggcacaaggt ggttaccgcc tagcgcggcc
     5521 agcggaagaa attactttgg gtgatatcat ccgcgttttg gaaggccccg tggcccctac
     5581 agattgtgtg attaagggtg ctgctggtaa gaatgataaa tgcaaccgtg ccactgcctg
     5641 tgtgatgcac catgtctgga gtcgcttaag cgatgagata aatgatcttc tcgacagtgt
     5701 cactttggca gaaattgtgg aagaacagcg cagactaact ggtgagggat acatgtacta
     5761 catttaaaaa aaaaggtatc gtcaaggcat tccctccact gggagggcgc cacgaatgca
     5821 aaaacctgct aggcgagatc cccacgactg ggatgctcaa ggcggcaaga aacggatggc
     5881 atgagtatga acaaaatttt agacttgaca ggaagcgtga caacagcggc gggtgagatg
     5941 accgtctcct atacagcagc tgggctttat gccctggaac tgcctggttt tgcggcgggc
     6001 agttatcttc ctgtcagcag cagtgagccg gcatggttac agaagctggc acttgattta
     6061 caaaactact ttttggggca gacagttcac tttacctgtc cttgggatga ggccaattac
     6121 ccgccctttt atcagcgtgt tttacaggca gccgcacaaa tcccttatgg tgagtgtcgt
     6181 acctacaaat ggttggcgca gcaagcaggc tcccctggcg cagtacgtgc agcaggtcag
     6241 gccatggccc ataatcgtac tcctttggtg attccctgcc atcgggtact gcgcagtgat
     6301 ggcaaattag gcggttttgc gtacggtttg tcttggaagg aaagactact aacattagag
     6361 aaagcagcga gggaggacct aaataatgca aattgacccc attgcctttc gactttttgg
     6421 tattcctatc tattggtatg gtgtgatgat tagttcggct ttgttatttg gtaccctggt
     6481 ggcgatgcgt cgtgcgccgc agtatggtgt tgatgaagat gatctactgg gttttctctt
     6541 attggcagta ccgctggcca ttgttggtgc ccgggcagtt tttgtggtgg aaaactggga
     6601 tacaatggcg ggtgattttt tagcgatgat taacctgcga cagggtggct tgtccattca
     6661 tggcgccctg attggtggca ttatcgccgg tttattttat acacagttta aaaggcttag
     6721 tttttggagc atcgcggata ttactgcccc tggtttaatt ctcggtcagg ccatcggccg
     6781 ctggggtaat tattttaacc aggaggccta tggtatacct accgatttac cttgggcact
     6841 atatatcgat ggtgcttacc gtcatcctat tttcttttat gaatttgtct ggaatttgct
     6901 tgtttttgcc tatttaataa ggattagtaa aaaagaaaag acgcccggcg gtatcttttt
     6961 gcgttatctg gttggctact ccattggtcg cttctttatt gaagggatcc gtgccaatac
     7021 tgttttttgg ggtaatttac gtttaggaca gatagtcagt gtgctgctga ttgtaatcgg
     7081 tttgctgctt ttatggtggc gcaaacggca gactaaagat gaggggggta ttgtgcgcta
     7141 atgcgttatt taacggcagg tgaatcacat ggcccggcca ttacaacaat tattgagggt
     7201 ttaccggcca atttacccct ggaccaactg gagattaatc aagatctaac tcggcgacag
     7261 cagggctatg gccggggcgg aagaatgaaa attgaaaagg atcaagtaga aatcctggct
     7321 ggtttacgct ttggtaaaac cctgggtagt ccgctgacgc tgcaggtaat aaacaaggat
     7381 tatgcccagt ggtcagataa aatggcacct tttggggaac agccccctga aatagctttg
     7441 gtaacgcacc cgcgtccagg tcacgctgat ctgcccggtg cgttaaaata taatttagca
     7501 gatatccgta atgttttaga gcgggctagt gcccgagaga ctgtagcccg agttgcggtg
     7561 ggcgccgtgg cgaggtcgtt attaagggcc tttgatatcc agatatattc ttatgtggaa
     7621 gccatcggcc aagtgtcggc agccgcacaa aaagtggctg ctttagtagc agcaaattat
     7681 gcaggggtag agaattcacc ggtgcgctgt cctgacccta cggcggcgca gcaaatgacg
     7741 aaggccattg acgaagctaa agctgccggt gattccctag gcggcatttt tgtggtggct
     7801 gccttgggtg tacctgtggg cctgggtagt catgtgcagt gggatcgtaa attggacgcc
     7861 cgtctggcgg ctgccctgat gagcattcag gccattaagg gagtggaaat tggtgagggc
     7921 ttcagcctgg cagcgcagcc aggttcagag gcgcatgacg aaatatatta ccatcacagt
     7981 aaaggctact atcggcagac taatcatgcc ggcggtattg agggtggtat gagtaacggt
     8041 gaggccatcg tctgccgggt agcgatgaag ccgataccta ctttatctaa gcccttacaa
     8101 agtgtgaata tgcaggatca ccgcccctac acagctgctg tggaacgtgc tgacagttgt
     8161 gctgtaccag cggcagctat cgtgggtgaa gctgtaatgg cttgggaact agcccaggct
     8221 ttacgtgaaa aattgggcag cgactccctg gaagaaatgc aggataacta ccgtacctac
     8281 ttaaaaacaa tagcaaagcg gtagacggag gtcatgatga aaactgtaca agttagcctt
     8341 gccgaacgct cttatcctat ttttattggc aaagatctat ggtctgaact gccgcagcag
     8401 ctgcctcttg ccttggctcc tgcttccgca ctactagtga gtgatacaaa tgtttatccg
     8461 ctctatggcg ataaaataag tgcagccttc actgcagctg gctttaaagt aacgccggcg
     8521 gtagttcccg ccggtgaagg tagtaaaacg ctcgcccgtg cagaagaact ttataccgcg
     8581 tccattaagg caggacttga ccgtgccagc gtgattgtgg cgctgggtgg gggtgtggtg
     8641 ggtgacttgg cggggtttat tgctgctact tatctgcgtg gtgtgccttt tgtgcaaatt
     8701 cccacgtctc tgcttgctca agtagacagc agcgtgggcg gcaaagtggc agtaaatcac
     8761 cctttaggca agaacctgat tggcgccttt tatcaaccta aactggtctg gatagaatta
     8821 gaggttttag ccaccttgcc gcagcgggag tttttagccg gagctgccga agtggtcaaa
     8881 tatggcgcga ttttaagtga accgctttgg acgaagctag agcaatcctg gccggatttt
     8941 ttagcacaag agcaagccgt tttaacgcaa gtgattattg attgctgcac actgaaagcc
     9001 gaggtcgtag ctgcagatga gcgggaaaca ggctggcgga cgctgcttaa ttttggccac
     9061 actctcggtc atgcgctgga agctgccacc cgttacactt actatttgca cggcgaggcc
     9121 gtccttgtgg gtatgacgct ggctgttcag ctggctttgc agcagcaaat attagcgcca
     9181 gctgccggag tgcgcctgct ggcactgttt agacgtcttg gtattaagcc ggcaccgccg
     9241 accttaacgg ccgctcctgt gctggccgca ctgcagcagg ataaaaaaag ggtgggaaaa
     9301 aagctgcctt ttatttttcc tctacaaatt ggtcaggcca gcacatttga cagcatcccg
     9361 ccggtactgc taagcagtat gctgcaagag tatttaaatt gacaacccct aattaagcgt
     9421 ttataattta ctgaggcact gggggtagtg gcaccggagt aatctggaag agtaccctct
     9481 tttgccgttt aactgtattc tcactaccta aacaaaatta tttagataaa acaagggggt
     9541 acccggatga gaaggaatcg tcttcttatt ttaattttaa taatagggct gctcttagtg
     9601 ggcagtgccg ccgctatttg gcaggccgct aattctttaa atctcggtct tgatttgcgt
     9661 ggcggcgttt atgtacttta ccgcgcagcc gagactgaag gtgcagctga cgacgcagat
     9721 aagcttgccc gtgccattac tattattcgt acccgcgttg atgcgttagg tgtggccgaa
     9781 cctaatattc aaaaagaggg tcaggatcgc attcgtgtag aactgcctgg tattgaagac
     9841 cagcgcgtgg cgcgtgaggt gataggcaaa acggctctct taaagtttgt cggacctgat
     9901 ggcgaaacaa ttgtctctgg tgacgaatta aaagatgctt ttgtgagcta tgatcaatat
     9961 aatcgcattg ctgtttcctt ggagtttaat gcggtaggca gcgaaaagtt tgcccaggcc
    10021 acggaaaaat tattaggtca acagattaac atctatcttg acgaagactt aatttctgac
    10081 cccggcgtaa acactgtcat tcctgatggt aaggcgcaaa tcacgggtaa ttttacagca
    10141 gaagaagcct cgaatttagc tctgcaatta cgttccggtg ctttgccggt taaattagag
    10201 gagctggaga ttcgcggtgt cggaccgcag ttaggtcgtg attccttagc ccgcagtgta
    10261 cgtgctggta ttgccggcat cattctggtc ctcctgttta tgttggtgta ttatcgtgcc
    10321 cttggtttaa tggcgggtgt gtcactggtt gtctatgccg caattgtttt aggggtaatg
    10381 agtgcgctta aggttacttt aacattaccc ggtattgccg gtttgatttt atctgtgggg
    10441 atggctgttg atgccaacgt cattattttt gaacggatca aagaagaatt aagcaatgga
    10501 cgcacaatgc gaccagcggt ggaagccggt ttcacccgcg ctttccgctc aatttttgat
    10561 tctaacgtga ctacgctaat tgctgccgtt gtgcttttcc aattcgctca aggttcagta
    10621 cgcggttttg ctgtaacttt aagtgttggt attttagtaa gtatgtttac ggcgcttgtg
    10681 ctgacacgtt tcttgctgcg ccaggccgcg ggtacggcgc ttttacctac tccgcagcat
    10741 ggcctgcaga cagaaacctc taccacaaag cgtggtccaa attatattgg cctgcgtaaa
    10801 gctgccctga ttttctcaat cctattaatt ttggcgggca tgacttcatt ggcaacccgc
    10861 ggattaaact acggggttga ttttaccggc ggcacactgc tgcaaattga tttggggcaa
    10921 gaatttacgc ttgatgaagt gcgtgatact ttagctccct ttgatctaga agctgctaca
    10981 gtgcaaaagg taggctcaga aacactggca ggaaacgagt cagagcaaaa gtcacaggaa
    11041 gtgttgattc gtacccccga attaacagca gcacaacaag atgaagtatt cgaggccttt
    11101 caggagcgtt ttgctatagc agaggatgcc ttattaagag tggaaaatgt gggggcagca
    11161 gtaggcgatg aattacaacg ccaggcggtc attgctcttc tgctagccag cctaggtatg
    11221 atcatttata ttaccattcg ctttgagtat cgctttgcca tcaccgccat cattgccctc
    11281 ttgcatgatg cagccattct cctgaccttc tttgccctct tccgtcagga actaaatggt
    11341 acctttattg ccgcttcact gacgattttg ggttattcca ttaacgatac gattgtcatt
    11401 tatgaccgca tccgcgaaaa catgaagtat cagcggaagg aaagcctggt cgagattgtc
    11461 aataacagta ttgcacagtc attaaggcgc acgattaaca cttcacttac caccctctta
    11521 gtgctggtga cgctgttgat tttcggcggt gtcacactgc gtccctttat tagtgcctta
    11581 gtggtgggtg tagtcttcgg tacctattcc tcgatcttca tcgcctcacc cttgtggtta
    11641 tggtggaaag aacgggaagc aaagaaaaaa gcactaactt agagctttat tgaaataact
    11701 ttagtgcgcc tgccccggta ggcgtacttt ttattttgcc gtcgtcctgt tatgttcata
    11761 tttaaagtcc aattgcgcag aataaaggaa tttactccaa acttgacgaa tcttttactt
    11821 gctttacttc gtgtttgctt tgcctttaaa agatggaggg atgaaaaatg cagcttggca
    11881 ttatagtagg tcttttattt ggtgtcttaa ttgccttatt tgcttttgtt aaccgcgcga
    11941 cagtggtggt taattacttt ttcgggcaag tggaagctag tgtagccttg atcattcttg
    12001 cctcggcgat gtccggggcg ctggcagtag gactatttgg tttaatcaca caaattcgca
    12061 ctggctttac tatctggaat tataaaaaga aaaccaaatc ttttgctaaa gaagtcgaag
    12121 aattaaagac acaaaaacag gctttgcttg atgacctagc tgctttacat gcagaatctg
    12181 aactgttgct gcagcaggag aagcagagtt tggagcagca acaaaacaat gtagagacgg
    12241 ccgctgaaat cgccgccgtt gaagcagcag aggagactag taatgacagt gaataaatgg
    12301 gcttgggctg tatcaaaggt ggatcagcag gctgtgcacc agctgacgca agagttagac
    12361 atccatcgac ttacagcaac caccctttat caacggggtc tccatagccc ccaggaagca
    12421 cgagcgtttt tatcgtgtga cctgtcggac ctaggagacc ctttttccat ggccggggct
    12481 gctgccgcgg cagtaagaat agcggcagca atagaacaaa atgaaaaaat actgatcttt
    12541 ggtgattatg atgccgatgg cgtgactgca accgcactat tagtgcgagc gctgcaaaat
    12601 ttaagcgctg acgtggcata ctacataccg agtcgttttg ccgaaggtta tggtctacaa
    12661 gctgcggtac tggaagagta tgccgctgca ggtgtccagc ttgtgattac agttgactgt
    12721 ggcattaatt cttttgccga gatggaattg gccaagcaaa gaggttttga cctgattatt
    12781 accgatcacc atacttgttt ccccggcaca cgcaccgcca tggcagttct taaccctaaa
    12841 cagactacct gttcatatcc agaaaaacaa ctagccgggg tgggtgtggc ttggaatttg
    12901 gttagagccc tctatgccac actgcagctg ccggttgagg caacctacga ctatttagat
    12961 cttgtggctg tgggtacgat tgcagatgtg gtgccgctga tcggtgaaaa tcgccttctt
    13021 gtcaaacaag ggttggcacg tttaagtgcc aatcctttac ccggacttgc tgctcttgcc
    13081 cgggtcagtg atttaacaga tactaaacta acagctacgc aggtagcctt tactttggcc
    13141 ccgcgactta atgctgctgg gcgcttggga aaagctgatc cagctgtgga gacgctgctt
    13201 gcagctccgg cggaggctga tgcttgggcg agagaattag aggccatgaa ccgccaacgc
    13261 cagcaaattg aaaaagtgat tactgcagag gccagagtat tggccgaagc tcaaactagt
    13321 gcccctgctt tagtgctgtg gcaggaaggc tggcatgccg gtgtaattgg catcgccgcc
    13381 gggcgcttgg ccagagaatt taagcgcccg gtagtcttga ttgctctaga gggcgcggaa
    13441 ggacgcggct cgattcgttc tattcccggc tgcaatgtcg ttacagcact acaggattgt
    13501 gccagtcatc tgctaaaata cggtggacat gcagaagcag ctggtttaag cattgccgct
    13561 gcagagctcg aggcttttcg ccaggccttt tgccaggctg taagagacca ggcatgtact
    13621 gaaccaattt tgcctgtagt agcagagacc aacttctcag agcttacttt agcactggta
    13681 aaggagttag agatcctagc accgttcggc caaggaaata gagaaccagt ctttctggtc
    13741 aacggggctg aagtgattaa tacgcagcga gtcggggccc agcgtgatca tctgcgttta
    13801 accttgcggc aagaggccgt tgtgcaaaaa gccatctatt ttggcggcgg agagcagcag
    13861 ttaaacccgc gtgaacgcgt ggagctaatt gtcatgacaa gccttaatca ttggcagggt
    13921 cgtacactgc tttcactgca ggtaaaagat ttgcgtccag ctgccgctga ccagcaccgg
    13981 acaccgacca tcatagatca acgtaataga aagctaagtg aacaaaggct ggcgcgtttg
    14041 gccgcagaaa agaacttatt aatttgggtt aatactaaag cggcccaaac cgggctggag
    14101 aaaacattag cgcggggtgc cgcagtaaca cagctgggac gtaaagtcga ttacgcacga
    14161 acttatgatg cactactctt ttaccacctg ccatatcaaa gaaaagcttg tgagcagctg
    14221 ctgcaaaact tgtcctttaa tgatgcccct caggtttatc tttgttatgg gactgaggaa
    14281 gaagctttaa atgaaaaaat ctttgcggcc acggtgccgg ctgcagaaac attacagcag
    14341 ttggcaagct gtgtctcgcc ggctgaacca aatttaacag ctggtaaagt agaacataag
    14401 ctcaagcagc ccgtcacaca atatctcatt aaagaggcac aggctgtttt tacggaaata
    14461 gcagctggac aaaataccac ttggccaccg caagagagtc aactgcagca gtcacctacc
    14521 tatcgccatt atgatcgtgt gttaacacgt ttccgtgctt accagaattt ctggcggcag
    14581 gcaacagcag ctgaaatcac aacttatcta cttaacccag aacagttaac cctggaggaa
    14641 gaggagattt aacgagatga gtctgcaagc attaaaagaa ataattaaag gttattatcc
    14701 ggtagaacca aattgggacc tgctcgagaa gggctacaac tttgctgtaa atgcacacca
    14761 aggtcagaaa cggttttccg gtgaacgtta tatcattcat cctctaggag tatccatcat
    14821 cctagccgag ttagagttgg acctggatac cattgttgcg ggtcttttac atgatgtcgt
    14881 ggaagatact gaggtgacac tggaggatat tgaaaccgaa tttggtgcgg atatctcttt
    14941 gctagtagac ggtgtcacca aactttccaa gttagagttt aaatccaaag aagagcggca
    15001 agccgagaat ttgcgcaaaa tgtttatggc gatggctaaa gatattcgcg tgctgttaat
    15061 taaattagct gatcgcacgc acaatatgcg tacgctaaaa tacttaacgt cgattaagca
    15121 gcaggcgatt tcccgggaaa ccttagaaat ttatgctcct ttagcccatc gtttagggat
    15181 ttataaaatt aaatgggaat tagaagattt agcctttcgt tatctggaac gagatcgcta
    15241 ttatgcttta gttgatagat tagccaagaa acgacaggaa agagaagctt ttattacgca
    15301 gatcatggga gcactgcagc ctaagctgca ggacgtcaat attaacgccc aaatctctgg
    15361 gcgtcctaaa catttgtaca gtatctcgca aaaaatgaag gaacagaata aagagttcca
    15421 tgaaatctat gatttaacag cgatccgcat tattgtcaac tcgctcaaag attgttatgg
    15481 tgccctaggg attgtccata ctttatggaa accaattccc ggtcgtttta aagactatat
    15541 cgccatgccc aaatccaata tgtatcaatc actgcatact ttggtgatgg tcggtaaaaa
    15601 tgagctgctt gaagtgcaaa tccgtacttg ggaaatgcac cgtactgctg aatatggtat
    15661 tgccgctcac tggcgctaca aagaaaaaaa tgatagtaaa caggaagagg aatttgagca
    15721 gaagctgact tggctgaggc agatcatgga actgcagcag gatagtaagg atgccgcaga
    15781 gtttatggaa aatgttaaaa ttgatctctt tgctgatgaa gtctttgtct ttacgcccaa
    15841 aggtgatgtg attgacctgc cagccggttc aattcccctg gactatgctt acaagatcca
    15901 tacagatatt ggccaccgct gcattggtgc ccgtgttaat ggccgtctgg taaccctgga
    15961 ttatgaactc aaaacgggtg atatcgtgga gattatgacc tccaaacaag gttcacccag
    16021 tcgcgactgg attaatatgg tgaaaagttc cggcgctaaa gctaaaattc gcgcttggtt
    16081 taaaaaggaa cgacgcgacg aaaacctggc aaaaggcaaa gaactactgg agaaagagct
    16141 acgtaaacaa gagctggatc cccatctcta tttgaaaaca gccttactgc aggagatggc
    16201 aaaaaaattc aacatccata gtgaagatga tgcctacgtg gccattggcc tcggtggtgt
    16261 cacagcccaa caggccgttt cacgtttaaa agaagaatac cgcaaaaaat atggtccacc
    16321 agagccggaa ccggaaaaag aatttatacc gcaaaaaaca gtcagcaaga caggtaaagg
    16381 cgtagtgatc gccggtatcg acagcatgtt actgcgcttt gccaaatgct gtacacccgt
    16441 acctggtgat cagatagttg gtgtggtaac acgcggtcgt ggtgtttcga ttcaccgcca
    16501 ggattgccta aatgttctca accacccgga tcaacaccgt ttgctggaag ctcactggga
    16561 agaaaatgcc gaaggtgcct accctgtgga aatagaaatc accggcatgg atcggccgca
    16621 gatcttaaag gatgtagtca ccgccgtctc ggaaagtaaa gttaacatta atgctttaac
    16681 tggccgcggt actaaagatc ggatgtcaat tattcagatg acactagtgg tcagggatct
    16741 cgatcatttg gaaaatgtca ttaatcgcct tagccgtgtc cgggatgtct atgacgtcca
    16801 ccgcattcat acttaaggag gatctcatgc gcgcagttat ccaacgtgtt gccgaggccc
    16861 gtgttgaagt tgacggggca ataattggtc aaataaacaa ggggttacta attttgttgg
    16921 gcatccaggc cgctgatcag gaagccgatc tgcgctacct ggtaggcaaa atagcccatc
    16981 tgcgaatttt tgaagatgag gcaggtaaaa tgaatctatc tttgttagat atcggaggca
    17041 gtgccctggt tgtttcccag tttaccctct atgccgatac ccgtcgtggc cgccgacccg
    17101 gcttttccta tgccgccggt cccgatcagg cagagccgct ttacgaacag ttttgcaatg
    17161 ttttagcatc tttcggcatc cctgtcgcca ctggcaggtt cggtgccacc atggatgtcc
    17221 agctcaccaa ccaaggtcct gtcaccatca ttattgacag cagcgacaaa acatcgtagg
    17281 ggcgaactgc gttcgcccgc acccatcacc ccaagcgtaa cgcccaaaca tccccccacc
    17341 gtaggggcga actgcgttcg cccgcacaga tcaagccccc aagcgtaacg cccatcaaca
    17401 tcaccccacg taggggcgaa ctgcgttcgc acgcactgat caagccccaa gggaaagcac
    17461 atcaacctcc cccacgtagg ggcgaactgc gttcgcccgc acagatcaag cccccaagcg
    17521 caacgcccat caacct
//
LOCUS       DASRMS010000098         1283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_225034, whole genome shotgun sequence.
ACCESSION   DASRMS010000098 DASRMS010000000
VERSION     DASRMS010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1283
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225034"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..767)
                     /locus_tag="VFC74_04470"
     CDS             complement(15..767)
                     /locus_tag="VFC74_04470"
                     /inference="COORDINATES: protein motif:HMM:NF013658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
                     /protein_id="HZK24623.1"
                     /translation="MKKYQCIVPLSGGKDSQACLKMAVKEFGAPNVLALFCDTQFEHP
                     ITYQHIKDTVKLYGVDLHTVTGGSVEEKILKYGRFPGGGARHCTDELKIRETKIFLKE
                     YSEKHGAVQLWYGMRTEESAARARRYEYKDPDDLYEPHEILNKYPKYLGKNGVRFRLP
                     IADWYTHEVMDYLAGEHNPLYDAGFGRVGCFPCLMGGDESKIKAFEYDEFGKSQLIKV
                     KQIEEKIGKSVFTSGIGFKYENDRQGCLLCEI"
     gene            complement(1017..>1283)
                     /locus_tag="VFC74_04475"
     CDS             complement(1017..>1283)
                     /locus_tag="VFC74_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24624.1"
                     /translation="DVPEPTPSNVELAAAHIARDAAKGLVLGSVDRETLEETVSAQSG
                     TFVTEKFSKGSRSISEGESLALALLSPWMAAKNKLVGFAFLSRT"
BASE COUNT          313 a          353 c          254 g          363 t
ORIGIN      
        1 gtttgtgcgc ttaatcatat ctcacacagc aagcagccct gcctgtcatt ttcatattta
       61 aacccaatgc cgctggtgaa tacgcttttt cctatcttct cttcaatctg cttgactttg
      121 atcagctggc tcttcccgaa ctcatcatac tcaaaggctt ttattttcga ctcgtccccg
      181 cccatcaggc acgggaagca ccctaccctg ccgaatcctg catcataaag cgggttgtgc
      241 tcacctgcta agtaatccat aacttcatgt gtgtaccagt ccgcgattgg tagcctaaac
      301 ctaactccgt ttttcccaag atactttggg tatttattta aaatctcgtg tggctcatac
      361 agatcatcag gatctttgta ctcgtagcgc cttgctctcg cggcactctc ctctgttcgc
      421 attccgtacc acaactgaac cgccccgtgc ttttctgagt attcctttag aaatatctta
      481 gtttcacgaa tctttagttc atcagtacag tgtcttgctc cccctcctgg gaagcggcca
      541 tactttaaaa tcttctcttc tacgctgccg ccagtcactg tatgcagatc gactccgtac
      601 agttttacgg tgtctttaat atgctgatat gtaatcggat gctcaaactg cgtatcacag
      661 aacaacgcta aaacattagg cgcgccgaac tctttaacag ccatctttaa acatgcttgc
      721 gaatctttac cgccagatag aggcacaatg cactggtatt ttttcataac atcacctttg
      781 ttattccacc gatgaaaggt tgcggcaact tgtcggtgtt acaagctttc ggggatcagc
      841 ctagccgcgc gataatcata ccataaaaaa gcccagcacc tattacagcg cgaggctttt
      901 tgaggaaaca aacacttaag cgattatgcg cggtgataac ggtcttagag caaaaccaac
      961 taacgcgccc cataataggt attgggaagc catcggctaa cggcacagtc aaattactac
     1021 gtgcgactta gaaaagcaaa cccaactagc ttattctttg cagccatcca cggcgaaagc
     1081 aatgctagtg caaggctttc accctcactg atactgcgcg atcctttgct aaacttctca
     1141 gtaacgaacg tgcctgactg tgcgcttact gtttcttcaa gcgtttcgcg gtctacgctg
     1201 ccaagcacca agcctttagc tgcatcgcgt gcaatgtgtg cggctgcaag ctctacgttg
     1261 ctaggcgtag gctcaggcac gtc
//
LOCUS       DASRMS010000099         3034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_359071, whole genome shotgun sequence.
ACCESSION   DASRMS010000099 DASRMS010000000
VERSION     DASRMS010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_359071"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1848
                     /locus_tag="VFC74_04480"
     CDS             <1..1848
                     /locus_tag="VFC74_04480"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C-terminal helicase domain-containing protein"
                     /protein_id="HZK24625.1"
                     /translation="VTIARSRRHIQQFYDTTEIGAFPERLTPKSYHVPITHMPGAVEL
                     NEIFAQLSALNLAVYAPMSYLLPSRIKKYEELYDTQVDGGRSRLRQVDREKSLRALMT
                     TNLLKRLESSVHSFRLTLQSLQDTIVQTLTIIEHFKQQAEQHLAAEEVGLYSADSFTE
                     EELDADNPFAVGKKIRIDLQDLDLPSWEFELRQDLAGLNALIQSVAPITPEHDAKLQH
                     LAELIQDKIEQPLNEGNRKILIFSAFADTTEYLYQHLTQQPALQALNLNFAQVTGAGN
                     PKSTLKTRRRAYDFQEVLTLFSPVSKEKDKVLPNEPGEIDILIATDCISEGQNLQDCD
                     YLINYDIHWNPVRIVQRFGRIDRIGSINQAIQLVNYWPDISLDEYINLKERVENRMAI
                     VDVTATGDDNLLDVNSEDAAYRKKQLKRLQTEVIDLEDTGAGISITDLGLNEFRMDIL
                     SHVQQHGSLEQAPKGLHAVIPAGNGLEAGVIFGLRNLNPDKHQDEQNRLHPHYLVYIS
                     EQGEVLASHLQAKQLLDLLRAACKGRSKPLLEACQAFNQRTHDGRDMQEVSDLLNQAI
                     HSMVEHKEESDIDSLFSGQQTTALVNSIQGLDDFELLGFIVVQDVQLEA"
     gene            1850..2512
                     /locus_tag="VFC74_04485"
     CDS             1850..2512
                     /locus_tag="VFC74_04485"
                     /inference="COORDINATES: protein motif:HMM:NF025693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4391 domain-containing protein"
                     /protein_id="HZK24626.1"
                     /translation="MLFAYPESTRVGRPLPKNTIYKNVAPTAKQRELLTQQIEKITWA
                     HKLAPDTLNIQASTDLPEIQVFQLRLKPDVAEVHESLLAYLDKAIPSNLIFEVHSVQG
                     IQTIACLKQFNQNGSVKCSPYLYGAVQPVDAERQALPISRDFNHLHQQLLATLLPYPL
                     QAGESLNAAIERTEKINKLEKTIQQLNTQLLNKSMQFNRRVELNTQLKSAQQEKIALL
                     NV"
     gene            2624..>3034
                     /locus_tag="VFC74_04490"
     CDS             2624..>3034
                     /locus_tag="VFC74_04490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002566135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HZK24627.1"
                     /translation="MEKLKMHSKDLTQDNLAKIQAAFPNCVTERKNAQGKIELAIDFD
                     LLHQELSANIVEGPQERYRLDWPGKREAMLTANAPIAKTLRPAREESVNFDDTENLFI
                     EGDNLDALKLLQETYLGKVKMIYIDPPYNTGNDFI"
BASE COUNT          897 a          754 c          670 g          713 t
ORIGIN      
        1 gtcaccattg cccgttcgcg tcggcatatt cagcagtttt atgacaccac tgaaataggt
       61 gctttcccag aacgcctaac acccaaatcc taccatgtgc ctattactca catgcctggc
      121 gcagttgagt tgaacgaaat attcgcacag ctttctgccc tgaacttggc cgtgtatgcg
      181 cctatgagct acttattgcc cagtcgtatt aaaaaatacg aagaacttta cgatacacag
      241 gtagatggtg ggcgcagccg gttacgccaa gtggaccgcg aaaaaagcct gcgcgcatta
      301 atgaccacca acttgcttaa gcgcttagaa agttcggtgc actctttccg cctaaccttg
      361 cagtcgctgc aagacactat tgtgcaaacc ctcactatta ttgagcattt taaacagcaa
      421 gcagagcagc acttagctgc tgaagaagtg gggctgtaca gtgcagattc cttcactgaa
      481 gaagagcttg atgctgacaa cccttttgct gtgggtaaaa aaattcgtat cgatttacag
      541 gatttagatc tgcctagctg ggagtttgaa ctacgccaag atttagctgg gctcaacgct
      601 ttaatccaat cagtagcgcc catcactcca gagcatgatg ccaagctgca acacttggct
      661 gagttaattc aagacaagat cgaacaaccg ctcaatgagg gcaaccgcaa aatattaatt
      721 ttttctgctt ttgctgatac cacggaatac ttatatcaac acctcaccca acagcctgcg
      781 ttgcaagcgt taaacctaaa ctttgcccaa gtcacggggg caggtaatcc aaaaagcacc
      841 ttgaaaactc gtcgccgtgc ttacgatttt caagaagtgc tcacgctgtt ttctccggtg
      901 tccaaagaaa aagacaaagt tctgcccaat gagccgggcg agattgatat tttaattgcc
      961 acggactgca tctctgaagg acaaaaccta caggattgtg attacctcat taactacgat
     1021 attcactgga atcccgtgcg cattgtccag cgttttggtc gtattgaccg tattggctcc
     1081 ataaaccaag ccatccagct ggtgaactac tggccggata tcagccttga tgagtacatt
     1141 aaccttaaag agcgcgttga aaaccgtatg gccatcgttg atgtgaccgc caccggtgat
     1201 gacaacctct tggatgtgaa ctccgaagat gcagcctacc gtaaaaagca gctcaagcgc
     1261 ttacaaactg aagtcataga ccttgaggat accggtgccg gcatcagtat taccgactta
     1321 ggcttgaacg aattccgcat ggatatattg agccatgtac aacagcacgg cagcctagag
     1381 caggcgccca aaggtttgca tgcggtgatt cccgcaggga atggcttaga agctggggtg
     1441 atttttggtt tacgtaactt aaatcccgat aagcatcagg atgaacaaaa ccgtttacac
     1501 ccacactact tggtgtatat cagcgaacag ggcgaagtct tggccagcca cttacaagcc
     1561 aagcagctgc tggacctgtt gcgcgccgct tgcaaaggtc gcagtaaacc tttactcgaa
     1621 gcctgccaag cgttcaatca acgcacccat gatggccgtg atatgcaaga ggtgtctgac
     1681 ctgttaaacc aagccattca ctcaatggta gagcacaaag aagaaagcga tatagacagt
     1741 ctgttcagcg gccagcaaac caccgcatta gtgaacagca ttcaagggtt ggatgacttt
     1801 gaactgctgg gctttattgt tgtacaagac gtacaactgg aggcttaagg tgttatttgc
     1861 ttaccctgaa tcaacccgtg ttggccggcc actgcctaaa aatacaattt ataaaaatgt
     1921 tgcaccgact gccaagcaac gcgagctact cacccaacaa attgaaaaga ttacctgggc
     1981 gcataagctc gcgccggaca ccttaaatat ccaagccagc actgacttgc cggaaattca
     2041 ggtgtttcag ctgcgactca agcccgatgt ggctgaagta cacgaaagcc tgctcgccta
     2101 tttagataaa gccataccca gcaacttgat atttgaagtg cacagcgttc aaggcataca
     2161 aactattgct tgcctgaaac agttcaacca aaatggcagc gttaaatgca gcccctatct
     2221 ctacggtgcc gtgcagcccg ttgacgctga acgacaggcg ttgcccatca gccgagactt
     2281 taatcatttg caccagcagc tgttggcgac gcttttgcct tatccattac aggctgggga
     2341 aagtcttaac gctgccattg agcgcaccga gaaaattaac aagctagaaa aaaccattca
     2401 gcagctaaac acacaactgc tgaacaaaag catgcaattt aatcggcgcg ttgagctgaa
     2461 tacacaatta aaaagcgctc aacaagaaaa aatcgcgcta cttaatgtat agccgaagtg
     2521 taaaacgagt tatttattta ggtgcgaagc cgcacttaaa gccacttaca gagtaaatag
     2581 tacataaagc caagctttat tatatgtatt gacaggaaac cctatggaaa agctaaaaat
     2641 gcacagtaaa gatttaaccc aagataatct cgctaagata caggcggctt ttcccaactg
     2701 cgtaacagag cgcaaaaacg cccaaggcaa gatagagctg gccattgatt ttgatctgct
     2761 gcaccaagag ctgtcggcca acatcgtcga aggcccacaa gagcgctacc gtttagattg
     2821 gccgggtaag cgtgaagcca tgctcaccgc caacgcgcct attgctaaaa ccctgcgtcc
     2881 tgctcgtgag gaaagtgtaa attttgacga tactgaaaac ctgtttatcg aaggcgataa
     2941 cctcgatgcc ttgaaactgc tgcaagaaac ctacctaggt aaagtcaaaa tgatctacat
     3001 agatccaccc tataacacgg gaaatgactt tatt
//
LOCUS       DASRMS010000100         2227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_627415, whole genome shotgun sequence.
ACCESSION   DASRMS010000100 DASRMS010000000
VERSION     DASRMS010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2227
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_627415"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /locus_tag="VFC74_04495"
     CDS             complement(<1..823)
                     /locus_tag="VFC74_04495"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZK24628.1"
                     /translation="MTIVADLHVHTQASDGILSAAAVVELAQAKGLAAMAITDHDTVD
                     GVQEAQSRGRKIGVKIIPGVEMSTEHQSREIHILGFYIDPQSKILNQLLQQLRSSRYE
                     RMKKMVSILRNLGYRIDIQEVLQKSGDAAPSRPHVARVLVEKGYFKTVSEVFHTLLDR
                     GLPAYVERFKLTPALAIEAIKAAGGLACWAHPGLVGNNELLPLLITAGLQGLEIFHPD
                     HHLQQEKQYLQLAAKHQLLVAGGSDYHGPEAGRERELAIRGLNAAGYHNFIGYCQS"
     gene            complement(820..1803)
                     /locus_tag="VFC74_04500"
     CDS             complement(820..1803)
                     /locus_tag="VFC74_04500"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HZK24629.1"
                     /translation="MSAQNNPLIIVDGHCDTVHHYRHTEGYNFAQRNKVGHLDLPRLQ
                     EGGVALQFFALFIEPEFKPHQSVTRALQLAEHFIAEMGKNSEQVQIVKTQQDLTDALT
                     QRKLAALMTLEGAEPLEGGVEILHILYRLGLRGVGLTWNQRNGLADGVGVGAGAGGLT
                     ELGKTMVREMNRLGIIVDGAHITPRGFYDLLETSSEPIVVSHANAHHLCAHPRNLTAE
                     QLQALRDHGGVVGLTFFPTFIVDDGPASLSQLLDHYCYIADHFGTDILGIGADYDGIE
                     STVAELPDVSHLPLLVKGLQERGFTESEVRQIMGENFLRIVRSTLPEEDKM"
     gene            complement(1950..2210)
                     /locus_tag="VFC74_04505"
     CDS             complement(1950..2210)
                     /locus_tag="VFC74_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_389580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HZK24630.1"
                     /translation="MEVLKVSAKSNPNSVAGALAGVLRERGHAEIQAIGAGALNQAVK
                     AVAIARGFVAPSGMDLICIPAFTDILIEGEERTAIKLLVQPR"
BASE COUNT          596 a          560 c          444 g          627 t
ORIGIN      
        1 agctttgaca atatcctata aagttatgat atcctgctgc gtttaaacca cgtatcgcca
       61 attcgcgttc tctgcctgcc tccgggccat gataatctga accacctgca acaagaagtt
      121 gatgttttgc tgctagctgc agatactgtt tttcctgttg taaatgatgg tcgggatgga
      181 agatctccag accttgcaaa cctgcagtaa tcagcagggg gagaagctcg ttattgccta
      241 ctaaacctgg atgagcccag cacgctaagc ctcctgctgc cttaatagct tcgatggcca
      301 gcgcaggcgt caacttgaaa cgctccacat atgccggcaa gccgcgatca agtaaagtgt
      361 gaaatacctc tgagacagtc ttaaaataac ctttttccac aagtactctg gccacatgcg
      421 gcctgctggg agcggcatcg ccggatttct gaagtacttc ttgtatatca atccggtagc
      481 ctaaatttct taaaatgctc accatttttt tcatgcgttc gtaacggctg ctgcgtaact
      541 gctgtaaaag ctgattaagt attttagatt gcggatcaat gtaaaaacca aggatatgaa
      601 tttctctact ttgatgttca gtactcattt ctacaccagg gatgatctta acaccgattt
      661 tgcgccccct gctttgtgct tcttggacac cgtctactgt gtcatgatcg gtaatcgcca
      721 tggcagctaa tcctttagcc tgtgctaatt ccacaactgc tgccgcactg agtatcccat
      781 cactagcttg tgtatgaaca tgcaggtcag ctacaattgt cacattttat cctcctctgg
      841 taaggtgctg cgcacaatgc gtaaaaagtt ttcccccatg atctggcgta cttcactctc
      901 tgtaaagcca cgttcctgca agcctttaac taatagcggc aaatgagaga catctggcag
      961 ttctgccact gtgctttcaa tgccatcata atcagcacca atacccaaga tatctgtacc
     1021 aaaatggtca gcaatatagc agtaatgatc cagcagctgt gacaaggaag ccggaccatc
     1081 gtcaacaata aaagtcggga aaaatgttag tcctactacc ccgccatgat cgcgtagagc
     1141 ttgtaactgt tcagccgtaa gatttcttgg atgtgcacat aaatgatggg cattggcatg
     1201 gctaaccacg ataggttcac ttgatgtctc taataaatca tagaaaccac gtggtgtaat
     1261 atgggcacca tcaacaatga tgcccaggcg attcatttca cgcaccattg ttttgcctaa
     1321 ctccgtcaga ccaccagctc ccgcgcctac tcctacgcca tcagctaaac cattgcgttg
     1381 attccaagtc aggccaacgc cgcgcaaacc gagacgataa agaatatgta aaatttcaac
     1441 cccgccttca aggggttcgg caccttctaa agtcattagt gctgccagtt ttcgctgcgt
     1501 taacgcatca gtaaggtctt gctgcgtttt aactatttgc acttgctcgc tattttttcc
     1561 catttcagca ataaaatgct ctgctaactg caaagcacgc gttactgatt gatggggctt
     1621 aaactctggt tcaataaata gggcaaaaaa ctgcagggcg acgcccccct cctgtaaacg
     1681 cggcaaatca agatggccaa ctttatttcg ttgagcaaaa ttgtatccct cggtatggcg
     1741 ataatgatgg actgtatcac agtggccatc aacaattatc agtgggttat tttgagcact
     1801 cacttttatc tcccctttcc actagtatat agtatcatac gtgagggtaa atgtaacagc
     1861 tagcttttgc taaatacttt agtttttgcc tgaaaagtta ttaaacgtga taacaaaaaa
     1921 aacacccatt ttcagggtgt tttgtttaac tacctgggtt gaacgagcag cttaatggca
     1981 gttcgttctt ctccctctat taatatatca gtaaaagccg ggatacagat taaatccata
     2041 ccacttgggg ctacaaaacc acgcgcaata gctactgctt taactgcctg attcagcgca
     2101 ccggctccaa ttgcctgaat ctcggcatgt cctctctctc taagcacccc tgccaacgca
     2161 cctgctacag aattcggatt agattttgct gaaactttta atacttccat cgctgaccct
     2221 cctgata
//
LOCUS       DASRMS010000101        12147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_360504, whole genome shotgun sequence.
ACCESSION   DASRMS010000101 DASRMS010000000
VERSION     DASRMS010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_360504"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(126..872)
                     /gene="map"
                     /locus_tag="VFC74_04510"
     CDS             complement(126..872)
                     /gene="map"
                     /locus_tag="VFC74_04510"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HZK24631.1"
                     /translation="MITLKSQREIELMRAAGRVVAEVLQTLEQAIYPGITTAELDKLA
                     EKQIRKAKCQPAFLGYQNFPASICTSVNEEVVHGIPSLRKLKEGDIVSIDVGAVYQGY
                     YGDAAATFAVGEVDEEDLRLLKATRESLLRGIDQALPGNRLTDISHAVQTYVEEKGFS
                     VVRNYVGHGIGNRMHEEPQVPNFGKPGRGPRLQAGMALAIEPMVNSGTWQVETLQDNW
                     TVVTKDRQRSAHFEHTIVITAGAPVIMTAL"
     gene            complement(885..2141)
                     /gene="secY"
                     /locus_tag="VFC74_04515"
     CDS             complement(885..2141)
                     /gene="secY"
                     /locus_tag="VFC74_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HZK24632.1"
                     /translation="MLETLRSAWKIKELRQRITFTLLMLVVFRLGSHIPVPGVDPGKI
                     TNFFQENNLFGLFNVFAGGSLERYTIFAMGIFPYINASIIVQLLGVVIPKFEEWSKEG
                     PEGQRKMQQVVRYGAIVLALLQATGMMLGIFKQAVLTPSFFTYLTIIVALVAGTAFLM
                     WLGEMITEKGIGNGISIIIFGGIVSQFGPGVLQIFELTRIGQLNIFVLLVTVLIVLAL
                     IVGIVALEEGQRRIPVQYAKRVVGRKMYGGQSTHIPFKVNQAGVIPVIFASTLLLFPS
                     TIAGFINHPVANWVGKVFQPGTAVHSVLYIMLILFFAYFYTAVQFDPSKIANDMKKNG
                     GFVPGLRPGRPTSEYLARVSSRLTLAGALALALIAFSPVLLQAIFNVNVLFGGTSMII
                     VVGVVIETMKQIESHLLMRNYQGFMK"
     gene            complement(2143..2583)
                     /gene="rplO"
                     /locus_tag="VFC74_04520"
     CDS             complement(2143..2583)
                     /gene="rplO"
                     /locus_tag="VFC74_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HZK24633.1"
                     /translation="MRLHELKSPQGARTAKKRVGRGIGSGHGKTATRGHKGQNSRSGG
                     GVRPGFEGGQMPLQRAVPKRGFTSIFKKQYNELNVDRLNAFADGTEVTPELLKQEGLI
                     KSLKDGVRVLGNGELEKALTVKAQGFSKTAAEKIEAAGGKVEVI"
     gene            complement(2598..2780)
                     /gene="rpmD"
                     /locus_tag="VFC74_04525"
     CDS             complement(2598..2780)
                     /gene="rpmD"
                     /locus_tag="VFC74_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HZK24634.1"
                     /translation="MAKQLRITLVNSVIGRIEPQRKTVEALGLKKLNQVVEHEDTPQI
                     RGMINKVSHLVKVEEL"
     gene            complement(2792..3292)
                     /gene="rpsE"
                     /locus_tag="VFC74_04530"
     CDS             complement(2792..3292)
                     /gene="rpsE"
                     /locus_tag="VFC74_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HZK24635.1"
                     /translation="MENFDPNKVELTETVVKINRVAKVVKGGRRFSFAALVVVGDGNG
                     RVGSGIGKATEVPEAIRKGMEDAKKNMIKVPIVGTTIPHPVTGVYGAGRVMLKPASEG
                     TGVIAGGPVRAVLEAAGVHDILTKSLGSANAINIVNATITGLKSLKTAEQVAKLRGLT
                     VEELLG"
     gene            complement(3314..3682)
                     /gene="rplR"
                     /locus_tag="VFC74_04535"
     CDS             complement(3314..3682)
                     /gene="rplR"
                     /locus_tag="VFC74_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003494926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HZK24636.1"
                     /translation="MFTRQDKNKQRKRRHLRVRKKLQGTKGCPRLNVYRSLKHIYAQI
                     IDDVAGVTLVAASSNEPSIREGKGSCGNKDAARQVGKLIAEKATEKGIKDVVFDRGGY
                     LYHGRVKELADGAREAGLKF"
     gene            complement(3697..4233)
                     /gene="rplF"
                     /locus_tag="VFC74_04540"
     CDS             complement(3697..4233)
                     /gene="rplF"
                     /locus_tag="VFC74_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HZK24637.1"
                     /translation="MSRIGRKPIQLPAGVEIINNDNVVTVKGPLGQLQKALPTVIEIK
                     VEDNQVVVSRHNDEKTQRSLHGLTRTLIANMVDGVTKGYSKTLELVGVGYRATKQGSN
                     VVLNIGYSNPVNVTPPQGIELEVPAPTKIVVKGIDKEAVGSMAANIRAIREPEPYKGK
                     GIKYDTERVRRKVGKTGK"
     gene            complement(4248..4646)
                     /gene="rpsH"
                     /locus_tag="VFC74_04545"
     CDS             complement(4248..4646)
                     /gene="rpsH"
                     /locus_tag="VFC74_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HZK24638.1"
                     /translation="MTMTDPIADMLTRIRNANMAKHDKVDVPGSKVKIAVAEILKNEG
                     FIKGYEFKKDNKQGTIKIFLKYGPNREKVLTGLKRISKPGLRVYAQKDELPKVLSGLG
                     IAIISTSQGIMTDKTARRAGVGGEVLCYIW"
     gene            complement(4677..4862)
                     /locus_tag="VFC74_04550"
     CDS             complement(4677..4862)
                     /locus_tag="VFC74_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009984979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HZK24639.1"
                     /translation="MAKKSKIAQANRTPKFKVQGYNRCKLCGRPRAYLRKFGMCRLCF
                     RKLAHEGKIPGVKKASW"
     gene            complement(4891..5433)
                     /gene="rplE"
                     /locus_tag="VFC74_04555"
     CDS             complement(4891..5433)
                     /gene="rplE"
                     /locus_tag="VFC74_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HZK24640.1"
                     /translation="MARLKDKYQNEVRPALMARFQYSNIFEAPRLEKVVINMGVGEAK
                     DNPKALDAAVNDLTIIAGQRPVITKARKSVASFKIREGMSIGCKVTLRGDRMYEFLDK
                     LMNVALPRVRDFRGISPKSFDGRGNYSLGLREQLIFPEISYDQVDKVRGMDIIIVTSA
                     KTDEEARELLREMGMPFRTA"
     gene            complement(5455..5787)
                     /gene="rplX"
                     /locus_tag="VFC74_04560"
     CDS             complement(5455..5787)
                     /gene="rplX"
                     /locus_tag="VFC74_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HZK24641.1"
                     /translation="MAKLHVKKGDQVLVLSGKDKGKKGKVLTSLPTAGKVVVEGVNIV
                     KKHARATQHMQAGIHEHEAPIYSAKVMVVCPNCKQATRIKKAAVKKANDKQAWVRACK
                     KCGELLDK"
     gene            complement(5811..6179)
                     /gene="rplN"
                     /locus_tag="VFC74_04565"
     CDS             complement(5811..6179)
                     /gene="rplN"
                     /locus_tag="VFC74_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015924311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HZK24642.1"
                     /translation="MIQAQTYLTVADNSGAKEIMCIKVLGGSVRKFGNIGDIIVASVK
                     EATPGGVVKKGDVVKAVIVRTAQGLRRNDGSYIKFDGNAAVLINDQKNPRGTRIFGPV
                     ARELRDKDFMKIVSLAPEVI"
     gene            complement(6347..6604)
                     /gene="rpsQ"
                     /locus_tag="VFC74_04570"
     CDS             complement(6347..6604)
                     /gene="rpsQ"
                     /locus_tag="VFC74_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HZK24643.1"
                     /translation="MERSNRKTRIGWVVSDKMDKTAVVTVERRTRHPLYGKIVRVTNK
                     YKVHDPDNNCSIGDKVKIMETRPLSKDKRWRLVEILEKTKL"
     gene            complement(6658..6852)
                     /gene="rpmC"
                     /locus_tag="VFC74_04575"
     CDS             complement(6658..6852)
                     /gene="rpmC"
                     /locus_tag="VFC74_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HZK24644.1"
                     /translation="MKAKELKEFSDAELTGKLSELKDELFNLRFQMATRQLENHMRIR
                     EVRKSIARIKTVQRVRELNS"
     gene            complement(6842..7276)
                     /gene="rplP"
                     /locus_tag="VFC74_04580"
     CDS             complement(6842..7276)
                     /gene="rplP"
                     /locus_tag="VFC74_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HZK24645.1"
                     /translation="MLIPKRVKFRRQHRGRMKGKSQVGNEIAFGEYGLQALEPAWITN
                     QQIEAARIAMTRFIKRGGKVWIKIFPDKPVTAKPAETRMGSGKGSPEFWVAVVKPGRI
                     LFELAGVPEDVAREAMRLAAHKLPIKTKFVKRLETGGDSHES"
     gene            complement(7254..7934)
                     /gene="rpsC"
                     /locus_tag="VFC74_04585"
     CDS             complement(7254..7934)
                     /gene="rpsC"
                     /locus_tag="VFC74_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HZK24646.1"
                     /translation="MGQKVSPIGFRLGVIKDWDAKWYADKNYTDQLLEDMKIRKALAK
                     YLDNAGVSRVEIERAANRIRISVHTAKPGIVIGKGGSGVEELRRKLEKLTDKQVHINI
                     IEIKRPELDATLVAENVASQLVKRVAFRRAMKQAIFRTMRAGAKGIKVMISGRLGGAE
                     IARTESQHEGTVPLQTLRADIDYGFVEAHTTYGRIGIKVWIYKGEVLPTKQRNTEGGN
                     PNANTKKS"
     gene            complement(7939..8280)
                     /gene="rplV"
                     /locus_tag="VFC74_04590"
     CDS             complement(7939..8280)
                     /gene="rplV"
                     /locus_tag="VFC74_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HZK24647.1"
                     /translation="MEAKASVKFIRMSPRKARLVVDQIRGRNAQEALAILQVLPNKPT
                     EPIYKVLHSAMANAENNYAMNRDDLYVAQVFVDQGPVLKRFRPRARGMASRIRKPTSH
                     ITIILKEKREG"
     gene            complement(8293..8577)
                     /gene="rpsS"
                     /locus_tag="VFC74_04595"
     CDS             complement(8293..8577)
                     /gene="rpsS"
                     /locus_tag="VFC74_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HZK24648.1"
                     /translation="MSRSLKKGPYVEEKLIAKVRQMNEKNEKKVIKTWSRRSTIFPEM
                     VGHTLAVHDGRKHVPVYLTEDMVGHKLGEFAPTRTFRGHGGHTERSTSLK"
     gene            complement(8605..9438)
                     /gene="rplB"
                     /locus_tag="VFC74_04600"
     CDS             complement(8605..9438)
                     /gene="rplB"
                     /locus_tag="VFC74_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020816386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HZK24649.1"
                     /translation="MAVKKFVPTSPGRRFMTVRSFDEITTTEPEKSLLEPLKKQAGRN
                     AQGRITVRHQGGGHKRKYRIIDFKRNKDGVPATVATIEYDPNRSANIALLHYADGEKR
                     YILAPVGVTVGTQVLSGEGADIKPGNALKLKNIPVGTIIHNIELKVDKGAQLVRSAGV
                     AAQLMAKEGQYAQVRLPSGEVRYISQECKATIGQVGNLEHENIDIGKAGRSRWLGKRP
                     TVRGVVMNPVDHPHGGGEGKSPIGRKSPVTPWGKPTLGYKTRQKNKKSDQYIVRRRNK
                     K"
     gene            complement(9462..9755)
                     /gene="rplW"
                     /locus_tag="VFC74_04605"
     CDS             complement(9462..9755)
                     /gene="rplW"
                     /locus_tag="VFC74_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010073740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HZK24650.1"
                     /translation="MMREARDIIRKPLITEKSTSLMGELKYAFQVDTRANKIEIKKAV
                     EELFNVEVKDVNTIHVTGKVKRMGVHSGRRPSWKKAIVTLADGSKPIEIFEGL"
     gene            complement(9752..10378)
                     /gene="rplD"
                     /locus_tag="VFC74_04610"
     CDS             complement(9752..10378)
                     /gene="rplD"
                     /locus_tag="VFC74_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HZK24651.1"
                     /translation="MPKVALYNQQGGQVGELELPDAIFAVKVNEAAMHQAVLAYLANQ
                     RLATAQTKTRTDVRGGGRKPWRQKGTGRARHGSIRSPIWRGGGVVFGPSGQRNYKQTL
                     PKKIRRLALKSALTTKVNAGSLIVLDTLVMDAPKTKEMVSMLGKLNAGRKTLLILDGP
                     HENVVKSARNLPGVKTTHARQLNVYDILNTDNLVVTRDAVTQMEEVLA"
     gene            complement(10445..11077)
                     /gene="rplC"
                     /locus_tag="VFC74_04615"
     CDS             complement(10445..11077)
                     /gene="rplC"
                     /locus_tag="VFC74_04615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011197190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HZK24652.1"
                     /translation="MEKAILGKKLGMTQVFTPEGQVIPVTVIEAGPCYVVQKKNAEID
                     GYEAIQVGFGNLKEKHITKPRAGHFAKANTAAKKYVREFRLADVSAYEVGQEIKADLF
                     SEGEWVDVTAKSKGKGFAGSIKRHGHSRGPMTHGSHYHRGPGSLGAVDAARVFKGHKL
                     PGRMGGDKVTVQKLRVVRVDTERNLLLINGAVPGPKGGLVTIKDSVKAGR"
     gene            complement(11113..11421)
                     /gene="rpsJ"
                     /locus_tag="VFC74_04620"
     CDS             complement(11113..11421)
                     /gene="rpsJ"
                     /locus_tag="VFC74_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_387996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HZK24653.1"
                     /translation="MAKQKIRIRLKAFDHQILDQSSSKIVETAKRTGAAVSGPIPLPT
                     EKTIYTVIRAPHKYKDSREQFEMRTHKRLIDILEPTPKTIDALMRLDLPAGVDIEIKL
                     "
     gene            complement(11553..>12147)
                     /locus_tag="VFC74_04625"
     CDS             complement(11553..>12147)
                     /locus_tag="VFC74_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013809477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZK24654.1"
                     /translation="YIPDPERDVDKPFLMPVEDVFTITGRGTVATGRVDRGVVKVQDE
                     VEIVGLSEKPRKTVVTGVEMFRKIMDQAEAGDNIGCLLRGIDRESIERGQVLAKPASI
                     KPHTNFMAEVYVLKKEEGGRHTPFFQGYRPQFYLRTTDVTGVITLPEGVEMVMPGDNI
                     QMKIELITSIALEEGLRFAIREGGRTVGAGVVTSILE"
BASE COUNT         2909 a         3153 c         2557 g         3528 t
ORIGIN      
        1 aggtaatact caccggagtc acggcctgca agagagtgaa ccagggcgcc aattattggc
       61 tgctgtttca tttatcaaac tcccttcata atgcaggttc cttaacgcaa aaccatgggt
      121 aacttttata gtgcggtcat aattacaggc gctcctgcag taatgacaat ggtatgctca
      181 aaatgagctg accgctgacg gtctttagtc accactgtcc aattatcctg tagtgtttcc
      241 acctgccaag ttccgctgtt caccatgggt tcaatggcca aggccatgcc ggcctgcagc
      301 cgcgggccac gacccggttt gccgaaattc ggaacctggg gctcttcatg catccggtta
      361 ccaatgccgt gaccgacata attcctgacg acagaaaagc ctttttcctc cacatatgtc
      421 tgaactgcgt gtgagatatc tgtcaggcgg ttgcctggca atgcttggtc aatccctcgt
      481 aggagagatt ctcgggtagc tttcagtaaa cgtaaatctt cttcgtctac ttcccccacc
      541 gcaaaggtgg cagcagcatc gccatagtac ccttgatata ccgcacccac gtcgatacta
      601 acaatgtcgc cttcctttaa cttgcgcagg ctgggaatcc catgaactac ctcttcattt
      661 actgaagtgc aaatggaggc ggggaagttc tggtaaccta ggaaggcggg ctgacatttt
      721 gctttgcgaa tttgcttttc agctaattta tctaattcag ccgtggtaat accaggataa
      781 atagcctgct ccaacgtttg cagaacttct gctactaccc tgccggcagc acgcatcagt
      841 tcaatctctc gttgggattt tagtgtaatc atggcgggcc tccattactt catgaagcct
      901 tgatagtttc tcattaacaa gtgactttca atttgcttca ttgtttcaat tactacaccc
      961 accacaataa tcatactggt gccgccaaac aagacattaa cattaaagat agcctgcaaa
     1021 aggacgggag aaaaagcaat cagtgctaat gccagagcgc cggctaatgt cagtctgctg
     1081 gatacccttg ccaaatattc tgaagtaggt ctgccaggtc gcagccccgg tacaaaacca
     1141 ccgttttttt tcatatcgtt ggcgatttta ctgggatcaa actggacagc cgtgtagaaa
     1201 taggcaaaga agagaattag catgatatag agtaccgagt gaacagcagt accgggctga
     1261 aagacttttc ccacccagtt tgccaccgga tgattaataa agccggcaat ggtgctggga
     1321 aacaacagta aggtagaggc aaaaatgacc ggtatcacgc ccgcttggtt tactttaaac
     1381 gggatatgtg tagactgacc accgtacatt ttgcgaccga cgacccgttt tgcatactgt
     1441 accgggatgc gacgctgacc ttcttctaaa gcaacaatcc caacgattaa agctaaaact
     1501 atcaacactg ttaccagcag cacaaaaatg tttagctggc cgattctggt caactcgaaa
     1561 atctgcagca caccaggtcc gaactgggaa acgatacccc caaagatgat gatggaaata
     1621 ccgttgccga tgcctttctc ggtgatcatc tcccctaacc acatgaggaa tgcggtaccg
     1681 gccactaagg ccacaataat ggtgagataa gtgaagaagc tgggtgttaa aacggcttgt
     1741 ttaaagattc ctagcatcat gccggtagcc tgcagcagtg ccaagacgat agcaccatag
     1801 cggacaacct gctgcatttt tctttgtccc tccggcccct ctttagacca ttcctcaaac
     1861 ttgggaatta ctacaccgag cagttgaacg ataatagagg cgttaatata agggaaaatt
     1921 cccatggcaa agatggtata tctctccagt gagccgccag caaaaacatt gaacaagcca
     1981 aacagattgt tctcttggaa aaagttagtt atcttacccg gatctacacc gggtaccggt
     2041 atatgtgagc ctaggcgaaa aacaactaac atcagtagcg taaaggtgat acgctgacgc
     2101 agttctttaa tcttccaggc actgcgaagc gtttccaaca tgctaaatca cctcgacttt
     2161 gccgccggca gcctcaattt tttctgcggc cgttttcgaa aagccttggg cttttacggt
     2221 gagagctttc tctaattctc cgttgcccag gacccgtaca ccatccttca aggattttat
     2281 taaaccctct tgttttaata gctcaggggt aacttcagtg ccgtcagcaa aggcatttaa
     2341 tcgatcaaca ttcaattcgt tatattgctt tttgaaaata ctagtaaagc ctcgtttggg
     2401 gactgcacgc tgtaaaggca tttgaccacc ctcgaaacca gggcgaacac cgccgccgga
     2461 acgggagttt tgtcctttat gaccgcgggt agctgtctta ccatgaccgc tgccgatacc
     2521 acgaccaacc cgctttttag cagtacgtgc accttgagga ctttttagtt catgcaggcg
     2581 catttattac acctccctta gagttcttct actttgacga ggtgggacac tttattaatc
     2641 ataccccgaa tctgaggtgt atcttcatgt tccacaactt ggttgagctt ctttaagccc
     2701 agcgcttcca ctgttttgcg ttggggctcg atacgcccga tcacgctatt caccagtgtt
     2761 atgcgtaact gtttcgccat gtgtttacct cctaccctaa tagctcttct accgtgaggc
     2821 cacgtaattt tgccacttgt tccgcagtct tcaacgactt caagcctgtt attgtggcat
     2881 ttacgatatt aatcgcattg gcagaaccga gtgatttggt gaggatgtca tgaacaccgg
     2941 ctgcttccag tacggcacgt acgggaccac cagcgatcac gcctgtacct tcagatgcag
     3001 gtttcagcat aactctgccg gcaccataga cgccagtcac aggatgtgga atggtcgtac
     3061 caacaatcgg tactttaatc atgttctttt ttgcatcttc catacctttg cgaatagctt
     3121 caggtacttc agtggcttta ccaatccctg aaccaacacg gccgttgccg tcaccaacaa
     3181 ccactaaggc ggcgaagctg aagcggcgac cacccttaac tactttggcc acgcggttta
     3241 tcttaacgac tgtttcagtc aattccactt tattggggtc gaaattctcc acgccttgtt
     3301 tccctccttc acgttaaaat ttcagaccag cttcgcgagc gccgtcggct agctctttaa
     3361 cccggccatg gtaaaggtac ccgcctctgt caaatacgac atctttgatg cctttttctg
     3421 tagctttttc agctatcagt ttgcctacct gacgggctgc gtctttatta ccgcaagaac
     3481 ctttaccttc tcggatggat ggctcgttgc ttgaagctgc aaccagtgtt acaccggcca
     3541 catcatctat aatttgtgcg taaatgtgct tcaggctacg atacacgttc aaacgcggac
     3601 acccttttgt tccctgtaac tttttacgta cgcggagatg tctccgttta cgctgtttat
     3661 tcttatcctg acgagtgaac aattcgtttc cctcccttac ttaccagtct taccgacttt
     3721 gcggcgaacc cgttcagtat cgtattttat acctttacct ttgtatggtt ctggctcacg
     3781 aatagctctg atgttagcag ccatgctgcc tacagcttcc ttatcgatgc cttttaccac
     3841 aattttagtc ggtgcaggga cttccaattc gatcccctgg ggtggggtga cattaacagg
     3901 gttagaataa ccaatgttaa gcacaacgtt gctcccctgc tttgtagcgc ggtaaccaac
     3961 acccaccagt tccagggttt tggagtagcc ttttgtgaca ccgtccacca tgttggcaat
     4021 taatgtcctg gttagaccgt ggagggagcg ttgtgttttt tcatcattat ggcgtgagac
     4081 aaccacttga ttgtcctcga ccttgatctc aattaccgta ggcaaggctt tttgcaattg
     4141 tcctaagggc cctttaacag ttactacatt atcgttattg ataatttcaa cgcccgcagg
     4201 cagttgaata ggtttgcggc cgatacggct catttcatat ccctccttta ccagatgtag
     4261 cagaggactt ctccaccaac cccagctcta cgagcagtct tgtcggtcat gatcccctgt
     4321 gaggtggaaa tgatggcgat accgagacca ctcagtactt tgggcagctc atctttctgc
     4381 gcatagacac gcagtccggg cttactgatc cgtttcagac cggtcagcac cttttcacgg
     4441 ttcggaccat atttcaagaa gattttgatg gttccctgct tattatcttt tttaaattca
     4501 taacctttga taaaaccttc atttttcagt atctccgcaa ccgcaatctt cactttagaa
     4561 cctggcacat ccactttatc gtgtttagcc atgtttgcgt tacgaatcct tgttaacatg
     4621 tcggcaattg ggtccgtcat ggtcattgca atcgaacctc ctttctctcc tatcccctac
     4681 cagctggctt tcttcacacc ggggattttc ccttcatggg cgagcttacg gaaacaaagg
     4741 cggcacatac caaacttgcg caggtacgcg cggggacggc cgcacagttt gcagcgattg
     4801 tagccttgta ccttaaactt aggcgtcctg tttgcctgag caattttaga ttttttggcc
     4861 actatttccc ctcctttttt gttttagttt ttaagcggtg cgaaatggca ttcccatctc
     4921 gcgtaataat tctctcgcct cttcgtcggt tttagcactt gttacgataa tgatatccat
     4981 cccacgaact ttatctactt gatcatagct gatttcagga aaaatcaact gttcgcgtaa
     5041 acctaacgag tagttacctc gtccgtcaaa ggacttcggt gagataccac ggaaatcacg
     5101 tacccgaggc agtgctacat tcatcagttt atcaagaaat tcatacattc tgtcaccgcg
     5161 aagtgttact ttgcacccga tgctcatgcc ttctcgaatt ttgaaactgg cgacagattt
     5221 tcttgctttc gtaatcacag ggcgttgacc agcgatgata gttaaatcgt ttacggcagc
     5281 gtcgagcgct ttaggattat cctttgcctc gccaaccccc atattgataa ccactttttc
     5341 cagtcgagga gcctcgaaga tgttgctata ttggaaacgt gccattaaag caggccgtac
     5401 ttcgttttgg tatttgtctt ttaggcgggc catacaatat tacctccttt ccggctactt
     5461 gtcgagaagc tccccacatt ttttgcaagc acgtacccac gcttgtttat cgttggcttt
     5521 tttaactgcc gcttttttaa tgcgtgttgc ttgcttgcag ttaggacaga cgaccatcac
     5581 tttcgcacta tagataggcg cttcgtgttc atgaatgccc gcctgcatgt gctgcgtagc
     5641 acgagcgtgc ttcttgacaa tatttacccc ttcaacaacc actttacccg ctgtgggtag
     5701 tgaggtaaga actttgccct tcttgccctt atctttacct gaaaggacaa gaacttggtc
     5761 tccttttttt acatggagtt ttgccactgt aaatgttgca cctccttacg ctaaatcact
     5821 tccggtgcca gcgatactat tttcataaaa tctttatccc gtaattcccg ggctaccggt
     5881 ccgaagatcc gagtaccacg tgggtttttc tgatcattaa tcagaacggc agcgttgcca
     5941 tcaaatttga tatacgagcc gtcattacgt cgtaacccct gggcagtacg gacgatgacc
     6001 gctttaacga cgtcgccttt tttgacaact ccgccgggcg ttgcttcttt tacggaagcc
     6061 acaatgatat caccaatgtt gccaaatttc cgtactgaac cgccaagaac cttgatacac
     6121 atgatctctt tggcgccgga attatcagca accgtcagat acgtttgggc ctgaatcatc
     6181 gtcaaacctc ctctaaaaca gatttctgct agctgatgtc ggatgtcaga cttgattaaa
     6241 tgctgagctc ggtcgttacc gatgcctgat cagcccacaa tcttcatgcg aaattgacaa
     6301 cttcaatttc tatgaatccg actctctgac ctccgacttc cagttgctat agctttgttt
     6361 tttctaggat ctctactaaa cgccatcttt tatctttact caaaggtctg gtttccatta
     6421 tttttacttt atcgccaatg ctgcagttat tatcgggatc gtgcactttg tatttattag
     6481 ttacacggac gattttacca tacaggggat ggcgggttct tctttccaca gtcactacag
     6541 ccgtcttatc cattttatca cttacgaccc agccaatacg ggtcttgcga ttactacgtt
     6601 ccaagagcat tgcctccttt cggacactat tgtttccggc caaaagcgac gcttttgtca
     6661 ggagttaagt tcacgtacac gttgtacagt tttaattcta gcaatacttt tgcgtacttc
     6721 acgaatacgc atgtggttct ctaactgtct agtagccatt tgaaagcgta ggttaaacag
     6781 ctcatctttc aactcggata atttccctgt taattcagca tcgctgaatt cttttaactc
     6841 tttagctttc atggctatca ccacctgtct ctagacgttt cacaaacttt gttttaatcg
     6901 gcagcttatg cgctgccaaa cgcattgctt cccgggcaac atcttcgggg accccagcca
     6961 gttcgaaaag aatccggcca ggcttaacca cggctaccca aaactcaggc gaacccttac
     7021 cactacccat gcgtgtttcc gcaggttttg ccgtaacagg cttgtcagga aagattttga
     7081 tccagacttt accaccacgt ttgataaaac gggtcatggc aatacgtgct gcttcaattt
     7141 gctggttagt aatccaggcc ggttccaggg cctgcaggcc atattcaccg aaggcaattt
     7201 cgttgcctac ttgtgatttg cctttcatac gaccacgatg ctgcctacga aacttaactc
     7261 tttttggtat tagcatttgg gttccctcct tccgtatttc tctgcttggt cgggagaacc
     7321 tcccccttat aaatccagac ttttatgcca atccggccgt aagttgtgtg ggcttccaca
     7381 aaaccatagt caatatcagc acgtaatgtc tgcaggggaa cagtcccctc atgttgtgac
     7441 tcggtgcgcg ctatttctgc accgcctagt cggccgctga tcatcacttt aattccttta
     7501 gcaccggcac gcatggtgcg gaagatagct tgcttcatgg cacgacggaa agcaacccgc
     7561 tttaccaatt ggctagcaac attttcagca actaatgttg catctagttc cggtctttta
     7621 atctcgataa tgttaatatg cacttgctta tctgtcaact tttccagctt cctgcgtagt
     7681 tcctcgacac cagatccacc tttaccaata acgataccgg gtttagccgt atgcaccgag
     7741 atgcgaatac ggttggcagc gcgttctatc tccacgcgag acacaccagc gttgtcaagg
     7801 tatttggcaa gagctttgcg gatcttcatg tcttcaagca gctgatccgt atagttttta
     7861 tcggcatacc atttagcgtc ccagtcttta atgacgccaa gccggaaacc gatggggctt
     7921 accttttgac ccacggactt atccctccct cttctctttg agaatgatgg taatatggct
     7981 agtgggtttg cgaatgcggc tggccatacc gcgggcacgg gggcgaaaac gcttgagcac
     8041 cgggccttga tcaacaaaaa cttgcgccac atataaatcg tcacggttca tagcataatt
     8101 gttttccgca ttagccatgg ccgaatgcaa tactttataa ataggctcag ttggcttatt
     8161 gggaagcact tgtaaaatag ccagcgcttc ctgggcattc ctgccgcgaa tttggtccac
     8221 aactaggcgg gccttccgcg gcgacatacg aataaattta acggatgcct ttgcttccac
     8281 ggcaattcct ccttatttca gcgatgtgga ccgttcagta tggccgccgt gtccacggaa
     8341 tgtccgtgtc ggcgcaaact cgccgagttt atgaccgacc atatcctcgg taagatatac
     8401 cggcacgtgc tttctcccat catggaccgc cagcgtgtgg ccgaccattt ccgggaaaat
     8461 agtggaacgg cgggaccagg ttttaataac cttcttttcg ttcttttcat tcatttgcct
     8521 aactttggca atcaatttct cttcaacata agggcctttc ttcagagacc tggacaagtc
     8581 ttgacgacct ccttcctgaa caatctattt tttattacga cgtctgacga tatactgatc
     8641 agacttttta ttcttctgac gtgttttgta gcccagcgtt ggtttacccc aaggtgtaac
     8701 cgggctttta cggccgattg gtgatttacc ctcaccacca ccatgtgggt gatcaactgg
     8761 gttcatcaca acaccccgga ctgttggacg cttacccaac cagcgtgagc ggccagcttt
     8821 accaatatca atgttctcat gctctaaatt gcccacttga ccaattgtcg ctttgcattc
     8881 ctgcgagatg taccgaactt cgccagaagg cagacgtact tgggcatact gtccttcttt
     8941 agccattaac tgcgcggcca cacctgctga acgtaccagc tgcgcacctt tatccacttt
     9001 taattcaata ttgtggatga ttgtaccaac cgggatattt ttaagcttca gcgcattacc
     9061 gggcttgata tcagcacctt caccggacaa aacttgtgtg ccaactgtga caccaaccgg
     9121 agctaaaata tatcgttttt ctccatcagc ataatgaagt agtgcaatat tagcggaacg
     9181 attaggatca tactcaattg ttgctacggt agcgggcacc ccatctttat tacgtttaaa
     9241 atcgataatc cggtacttac gtttatgtcc accgccctga tgacgtacgg taatccgacc
     9301 ctgggcattt ctgccggctt gctttttcaa gggctctaaa agtgatttct cgggttcggt
     9361 agtcgttatc tcgtcaaaag acctgacggt cataaaacgg cgtccgggag aggtgggaac
     9421 aaatttcttc actgccatat tctttctccc tccttcctgg gttacagacc ttcaaatatt
     9481 tcaatgggct tgctgccatc cgcaagggta acaatcgcct ttttccagct cggacggcga
     9541 ccggaatgaa cgcccatgcg ttttactttt cctgtgacat ggatagtgtt aacatcttta
     9601 acttccacgt taaacagttc ttcgacagcc tttttaatct caatcttgtt tgctctagta
     9661 tctacttgaa aagcgtattt cagctctccc atcaaactag ttgatttctc agttattagg
     9721 ggctttctga taatgtctct cgcctcacgc atcatgcaag cacctcctcc atctgggtca
     9781 cggcatcccg tgtcacaacc agattatcgg tgttcaagat gtcatagacg ttaagctggc
     9841 gtgcatgggt ggtttttaca cctggaagat tacgggcaga tttaaccacg ttttcatggg
     9901 gaccatcaag gatcagtaaa gttttacgcc ccgcatttaa tttccccagc atgctgacca
     9961 tttctttcgt ctttggagca tccatgacta acgtatcgag gactatcagt gagccagcat
    10021 tgactttagt tgtgagcgca gattttaaag ctaaccttct gatttttttc ggcaaggttt
    10081 gcttgtaatt acgctgccca ctaggaccaa agacaacccc accgccccgc caaatgggtg
    10141 agcgaatgga gccatgccgc gcacgtccag ttcccttctg gcgccatggt ttacgtccgc
    10201 cgcctcgtac atctgtgcgt gttttagttt gggcagttgc caagcgttgg tttgctaaat
    10261 aggccaaaac tgcttggtgc atggcggctt cattaacttt aacagcgaaa atggcatctg
    10321 gtaactctaa ttcaccgacc tgcccacctt gctgattata taaagctact ttcggcatca
    10381 caaatcctcc tttcttaact agaggtcaga tttaaaccag cctctaacct ctctttgtgg
    10441 acccttaacg tccggccttg actgaatcct taatggtaac caggccgcct ttagggccgg
    10501 gaacagcacc attaatcaac agcaagttac gctctgtatc caccctgaca actcgtagtt
    10561 tttgaacagt aactttatca ccgcccatac ggccaggtaa tttatgacct ttaaagaccc
    10621 gagccgcatc tactgcgcca agtgagccgg gaccacggtg atagtgtgag ccgtgtgtca
    10681 tggggccgcg actgtggccg tgtcgtttaa tagaaccagc aaacccttta cctttagact
    10741 tggctgttac atcgacccat tcaccttcgc taaagagatc tgcttttatt tcttggccta
    10801 cttcataggc actgacatca gctaaacgga attcacggac atattttttg gctgctgtat
    10861 tggccttggc aaaatgtcca gctcgtggtt tggtaatatg cttctctttt aaattaccaa
    10921 agccaacttg aattgcttca tagccatcga tctcggcatt ttttttctgt actacataac
    10981 agggtccggc ttcgataaca gtgaccggaa ttacttgccc ctccggagta aagacttgcg
    11041 tcataccgag ttttttacct aagattgctt tttccatgtt ttgcacctcc ttaaaaagct
    11101 ggtttccttt gtttacagtt tgatttcaat atccacaccg gctggcagat ctaatctcat
    11161 tagtgcatca atagttttag gagtcggttc aagaatatcg atcagccgct tatgtgtccg
    11221 catttcaaac tgttcacgag aatccttata tttatggggt gcccgaataa cagtataaat
    11281 agttttttct gtcggcaggg gaataggacc ggacacggct gcgccagtcc ttttcgctgt
    11341 ctccacgatt ttgcttgaag actggtctaa tatttggtga tcaaacgcct ttagacggat
    11401 acgtatcttt tgtttagcca tcattttccc tccttcgttc gcccgattat cagactactc
    11461 cacaaaaatt accgggattg gacccgcaac cttttgcttc atcggtagcc taggctgctc
    11521 aatggtctga acagcctagg aactgcatat aattattcca gaatgctagt tacaacaccg
    11581 gcgccaacag tgcggccgcc ttcacggatc gcaaaacgta gaccttcttc caaggcgatg
    11641 gaagtaatca actcaatttt catttggata ttatcgccag gcattaccat ttccacgccc
    11701 tcgggcaagg taatcacacc tgtgacgtca gttgtccgca ggtagaactg aggacgataa
    11761 ccttggaaga acggggtatg acgaccgcct tcttctttct ttaggacgta aacttcggcc
    11821 ataaagttgg tgtgaggctt aatgctggca ggtttagcga gaacctgacc acgctcaata
    11881 gattcacgat caataccacg cagcagacaa ccaatgttgt caccggcttc agcttgatcc
    11941 ataatcttgc ggaacatttc tacgcctgtt acgacagttt tacgtggttt ttcagataaa
    12001 ccaacgattt ctacttcgtc ttggacctta actacaccgc gatcaacacg accggtagct
    12061 actgtaccac gaccagtaat ggtgaaaaca tcctctacag gcattaagaa aggcttatca
    12121 acatcacgct cgggatcggg aatataa
//
LOCUS       DASRMS010000102        27646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_92913, whole genome shotgun sequence.
ACCESSION   DASRMS010000102 DASRMS010000000
VERSION     DASRMS010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 27646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27646
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92913"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..741
                     /locus_tag="VFC74_04630"
     CDS             61..741
                     /locus_tag="VFC74_04630"
                     /inference="COORDINATES: protein motif:HMM:NF019413.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HZK24655.1"
                     /translation="MKSIVITCATIRKEIDQAISKTGFTSPVLYLEAGLHNYPKKLHL
                     ALQELLNRVANVDQVLLVMGYCGNAVLDLEARDYRLLIPRADDCLTMLLGSQKRRLEV
                     QNEKQTYFFSPGWLDHFEDLEKDMVQGRARMEQRYGKEKARKLFREMFKHYRRAGLID
                     TGTFELDELLVKAQQRAAEMELDCEVIPGTMSYLEKFLAGPWEKKDFIILQPGEKVHY
                     SHLFGNEA"
     gene            793..2169
                     /locus_tag="VFC74_04635"
     CDS             793..2169
                     /locus_tag="VFC74_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZK24656.1"
                     /translation="MRQDLPAEEMRLILWTIINSVQDAISVVDEHGKGILFNHAYLQA
                     TGLQEADVLNKPAEVDIVEGESVHLKVLETGKPIRGVPMKVGRYKRHVMVSCAPLYID
                     NKLRGSVGIIHDVSEVRALSAELQRAKQKVRQLETKYTFDDIIGESTVLKQAIQLAKV
                     AAATPATVLLRGAGGTGKELFAHAIHHASPRQGEQFIRVNCSAFPESLLESELFGYSD
                     GAFTGARRQGKKGIFEEADGGTIFLDEIGTMPLSLQAKILRVLQEGEIVRIGEARPLT
                     VDVRVIAATNADLEKLIMENKFREDLYYRLNVFPIYIPPLVERKADIPLLAQYYLHAL
                     GEDYNRPDTELSSTAAKMLQEYHWPGNVRELKNVMARALINLNKDEKLVKTRHLMFSL
                     STSQQISSGRDVQVYDGETLAQQHARWEKNILLETLHSTKGNKTEAARQLGISVRSLY
                     NKLEKYGV"
     gene            2549..3052
                     /locus_tag="VFC74_04640"
     CDS             2549..3052
                     /locus_tag="VFC74_04640"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZK24657.1"
                     /translation="MHKKNIVLIGMPGAGKSTVGVLAAKVLGMAFVDTDLLIQEQEQR
                     LLQEIINQQGIKEFLKIEEEVLFGLQVENSVIATGGSAIYSLPAIKHLQNSGKLIYLK
                     ISLPELEERLGNMAQRGVVMGKGETLADLYAARTPLYEQAADRIVECSNLTTEEVITR
                     IIANLHN"
     gene            3117..3260
                     /locus_tag="VFC74_04645"
     CDS             3117..3260
                     /locus_tag="VFC74_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24658.1"
                     /translation="MKTKGENKSFFILIPPLAVIMIFGLLIVNIVLAIGILRFMKKCE
                     ERI"
     gene            3520..4911
                     /locus_tag="VFC74_04650"
     CDS             3520..4911
                     /locus_tag="VFC74_04650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK24659.1"
                     /translation="MQQENSHELGQTNIKKLLFKYSSPAIIAMLFNSLYNMVDTIFVG
                     RGAGTLAIAGLAISFPIQMLVMAIAQTVGIGAASIISRSLGAGDKKRAAQAAGNAFTI
                     VSILSIAFMVFGLTYLTPLLRLFGATEAILPYAREYMSVILAGSVFFSLAVCSNNIVR
                     AEGFAKVAMYSMLIGAIGNIILDPIFIFTFDMGIRGAALATIIAQFGSFLYLAVFFLR
                     GSSSVKISRQGLKINWQLVPEIFAVGASSFVRTVAGSLFSIVVNNSIAYYGADIHIAV
                     LGVANRLITFTMMPIMGIIQGLQPIIGFNYGAKKFARVKESLQLAIKVASAFALVGYL
                     GMMLFTEQFVRIFNNDPALVVEAVPIIRAMALLIPLIGFQMVGSSLFQAIGRAAPALF
                     LSMSRQLLILIPLVLILPLSMGLIGIFYAFPLSDLLSTIITSLWLRREMRIMSKEKGD
                     GPAKADDHLEKVS"
     gene            5038..5376
                     /locus_tag="VFC74_04655"
     CDS             5038..5376
                     /locus_tag="VFC74_04655"
                     /inference="COORDINATES: protein motif:HMM:NF014132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HZK24660.1"
                     /translation="MTVEEVLADLFAQLDQAPPELMAMDAVFELNILGAQAVTYQLKI
                     KNQQIEWAKGTPFLPSCIFYLSDTDFLKIAVGELNTKLAIMTGRIKIEGDYGQALQLS
                     AIIKKHYKPY"
     gene            complement(5465..6580)
                     /locus_tag="VFC74_04660"
     CDS             complement(5465..6580)
                     /locus_tag="VFC74_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HZK24661.1"
                     /translation="MTEEQKKLRKKQGGIIVVALLLLFLTAILAVEGVQVKFLAAWHS
                     WFTEELPAPGEGKLPNTDEDIEQPIPEEIDEDIEQPIPEKTDEKQEPDQMQEEPADIP
                     HDAETTPIIYVGQKLLLPFSGTKQTVSQVITQGVISSQQPQIALTFDAGWLFDQTNDI
                     LDVLDDYGIKSTFFLRALWAEAHPDLAREINRRGHRLENHSLTHGHLIKMTDDEVRKE
                     LQKSTSIIKEITGSQPRLFRPPYGEYHERLLQLVGEAGYPYTVTWTVDSHDWAAEIRG
                     QQVTTDYLVARVLNNASPNGIILMHLGGYQTVAALPRIITGLQDQGYRLVTVNEMLPP
                     TATEATSAYTVQPGDTLYAISRRYKITVQELIQLNNL"
     gene            complement(6774..7718)
                     /locus_tag="VFC74_04665"
     CDS             complement(6774..7718)
                     /locus_tag="VFC74_04665"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK24662.1"
                     /translation="MINTRRLCSILLVLTLALAGILTGCGNGDTSGDTPPDEKGVTLK
                     IGSQGYAEVEILAELTKILIEDRTPHKVEHIRNLGSSMAAHEATVSGQLDMCNNFTGT
                     LFLGLYEQGLTEEYRDPQKVWEFVHENLPKDHGLTVFPSYGYNNTFCVAVPRKWADEN
                     NINKQSDLAPFAKEMSLAVTPSWLNYPGQGYKEYTEIYGFSFKETVEMNRSLMYMAIR
                     NDEVDAINTYSTDGELVVENLKILEDDKGFNPPYYGILMVRTEVLEEHPELEEALQGI
                     ENLITTEQMQELNKKVSVDEMEPEDVAREHLKANGLID"
     gene            8048..9112
                     /locus_tag="VFC74_04670"
     CDS             8048..9112
                     /locus_tag="VFC74_04670"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF006707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HZK24663.1"
                     /translation="MNISELLGKDAQYLLEHQCDTIERARLHLPGPDFVGRVVAMTDR
                     PVPVLRSLQTMFNHGRLSETGYLSILPVDQGIEHSGGASFAPNPAYFDPEQIVKLAIE
                     GGCNAVASTLGVLGAVSRRYAHKIPFIMKLNHNELLSYPNTYDQVMFADVKQAWAMGA
                     VGVGATIYYGSEQSRRQIQEVTAAFRLAHELGMFTVLWCYLRNGSFKIDGVDYHSAAD
                     LTGQANHLGVTLEADIIKQKLPEVNGGYPAVSKQAGKTFGKIHDKVYSDLTTDHPIDL
                     ARYQVANCYLGRAGLINSGGASGKNDIVDAVRTAVINKRAGGMGLICGRKAFQKPMAD
                     GVKLLNAIQDVYLEAKITIA"
     gene            9297..9833
                     /locus_tag="VFC74_04675"
     CDS             9297..9833
                     /locus_tag="VFC74_04675"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HZK24664.1"
                     /translation="MTRKFATRDLVLIALFAALTSLLAYLIIPLPGGFPPLTGQSLAV
                     MLAGLLLGARNGAMSQLVYLLLGAAGLPVFAGGTAGVGVIVSPTGGFIWGFVVGAWVI
                     GKLTEDQKKLTWLRLVSAAVCGGVFAVYVPGVLQMANVLQITPSRALVAMLPYLPGDL
                     LKVFIAAALARKTMPQLQ"
     gene            9869..10516
                     /gene="thiE"
                     /locus_tag="VFC74_04680"
     CDS             9869..10516
                     /gene="thiE"
                     /locus_tag="VFC74_04680"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HZK24665.1"
                     /translation="MTRSFLQTDIYGITCEAMSAGRSNLETVRELLQAGVQIIQYREK
                     EKTKREKFAQCLALSKMCKKAGALFIVNDDVDIALAAQADGVHVGQDDLPADIVRALV
                     GSKVIVGVSAGNTKEAAAAVACGADYLGVGPIFATGTKLDAGAPIGLEFLAYLKARYT
                     LPLVAIGGINPENITQVFQSGADSAAMISALVSSSELGATVRATRQRLKEHGFAG"
     gene            complement(10491..11087)
                     /locus_tag="VFC74_04685"
     CDS             complement(10491..11087)
                     /locus_tag="VFC74_04685"
                     /inference="COORDINATES: protein motif:HMM:NF018844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HZK24666.1"
                     /translation="MRQYLKAVFNNKLFFVSLLVINFIGIVWGFWWYQPQFVTTPPYL
                     WPFVPNSPLAVLYFFLVLLRLRRGRRHYFLEGFAYFGLIKHGLWTVVIITVYQLSGYR
                     HFANILLWSGHLGMALEASLFWFYYRLPLNYAQATAIAAWYFFNDYLDYVVGIYPRVD
                     TTYISVAAIRNISVSFGIVLTILLFNSAWKFTQQSRVL"
     gene            complement(11262..11735)
                     /locus_tag="VFC74_04690"
     CDS             complement(11262..11735)
                     /locus_tag="VFC74_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK24667.1"
                     /translation="MFENTSYFTEVFQEIFPKLTHYLETEESKELTGLGITPGQINAL
                     LILFMQDGLTMGELSAEIYLAESAATRLVDRLVKLNLVKRQGDDRDRRVVRVYLTSYG
                     TQLASLVFERRNKRFNHLAARLTAEEREMLVLSVKAVLRVFAEFDLIKENKADEA"
     gene            11965..12573
                     /gene="coaE"
                     /locus_tag="VFC74_04695"
     CDS             11965..12573
                     /gene="coaE"
                     /locus_tag="VFC74_04695"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824148.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HZK24668.1"
                     /translation="MLVIGLTGGIATGKSTVADLFAKLGAYRIDTDHLARLIVVPGKP
                     AWQAIIQYFGEEILLPDGQINRSKLGKLVFSDAKKRQVLENITHPAIRELMQIKLSEA
                     RANGICLALVEVPQLFETDFHQDVDQTIVVTAGEEQQLVRLAVRDGLVGEDARQRLAA
                     QMPLSAKAARADYVIVNNGDLAETERQVKVLWQLLQRECVND"
     gene            12566..13126
                     /locus_tag="VFC74_04700"
     CDS             12566..13126
                     /locus_tag="VFC74_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HZK24669.1"
                     /translation="MTELIRGSRLLFMLIIIVLLVVFFRSPYFRRLSYPYHHRKIIEK
                     NALEFRVDPLLVAAVIHTESKFDVRAVSPKGALGLMQIMPPTAAWIAQKLKLGSLTKE
                     QILQPETNIRLGAWYLANLTQEFNGQLDLVITAYNGGPGQVNRWLASGVWSGRYADRA
                     EIPFSETREFLERVRTTYLQYQELYR"
     gene            13294..14049
                     /locus_tag="VFC74_04705"
     CDS             13294..14049
                     /locus_tag="VFC74_04705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HZK24670.1"
                     /translation="MAGHSKWANIKHKKARADAQKGKSFTKISKEIMAAVRQGGSDPN
                     GNFRLRLALQKARSVNMPNDNITRAVQKGAGELDGVNYEEIVYEGYGPAGTAVLLDIL
                     TDNRNRTVGEIRHIFSRHAGSLGATGCVAWMFNRKGYLAVAADGIDEDDLMLQALDAG
                     AADFEADEDGYEIYTAPDDFEAVKEKLEVAGIDFTEAEITMIPENTVVVTDLEDARKA
                     MILIELLEDHDDVQNAYTNLDIPAEVAQALENE"
     gene            14115..14492
                     /locus_tag="VFC74_04710"
     CDS             14115..14492
                     /locus_tag="VFC74_04710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24671.1"
                     /translation="MLKKKQPGIYTISIFSLGLLLGLVLGKFDLLPGATAAMPQAAEG
                     AILFGAADASQPGSATAVEYQGYLGVYEDHLAIYDGLPPHGTLQHVIADYEVRDDVRP
                     LLEAGIPFTDTYDLLRLLENYTS"
     gene            14547..15014
                     /locus_tag="VFC74_04715"
     CDS             14547..15014
                     /locus_tag="VFC74_04715"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HZK24672.1"
                     /translation="MRFSRELSGLAVIHYTSGRELGKVKEWLLDEYGETVVALVIEGS
                     GWLPQRRIYPYQDIFSIGNDVVLVSTEGLLAAGDPPQLESQATFRVLGKRMVSGDGNE
                     LGIVEDVLFEEESGHVTGWRLSAGLIDDLLQGRPVLEESLQLHIGEDALIIRD"
     gene            15031..15231
                     /locus_tag="VFC74_04720"
     CDS             15031..15231
                     /locus_tag="VFC74_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24673.1"
                     /translation="MKCPLCTSIDIGKVGTGQYYCWNCLLEFTMTSKNEFTAYYVDEE
                     GTLVNLSEMVQNNQLMEETVLG"
     gene            15291..16358
                     /locus_tag="VFC74_04725"
     CDS             15291..16358
                     /locus_tag="VFC74_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HZK24674.1"
                     /translation="MLTTKNYRSTYYRYVVLGITVALVLALLYWLSRTGAVLAAVLVP
                     FLLAIVLAYILNPVVEFLEKRRVPRTLGILLIYAVFFLAGTVFSLTVIPTLITELQKL
                     GEMIPQYTEQFQAFLLHLHSDYQRINLPESLRLALDQNLFALQDGIQAILERVTGTVL
                     SLFSNLMTILIIPLVVYYILRDMDNLKRSAVLLFPKKYRNWIISMGSEMDRTLGAYFR
                     GMLIICFLVGFLTYLGLKVIGLDFALILGVIAGLTNIIPYFGPIIGAVPAILLALLSS
                     SGLAVRVIVVYTLVQQLESQVISPQILGRSLGLHPLFVIFVLILGGRFLGLPGLIFAV
                     PFTAIARIFLKHAIDLAANRT"
     gene            16477..19095
                     /gene="alaS"
                     /locus_tag="VFC74_04730"
     CDS             16477..19095
                     /gene="alaS"
                     /locus_tag="VFC74_04730"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HZK24675.1"
                     /translation="MLSKDIRSKFLNFFQSKDHLVLPSFPLIPQNDPTLLLIGAGMAP
                     LKPYFTGEKTPPHSRVATCQKCVRTPDIERVGRTARHGTFFEMLGNFSFGDYFKEEAI
                     EWAWELVTTVYNLPEDRLYASIYLDDNEAFDIWHKKIGLSPDRIFRLGKEDNFWEIGQ
                     GPCGPCSEIYFDLGPEHGCGRPDCAVGCDCDRFMEVWNLVFTQFSCTAEGEYVPLAQR
                     NIDTGAGLERLAVVLQGVNNLFEIDTVRPLLDHFSEATATNYGQAADTDISLRIITEH
                     LRGISFMVGDGILPGNEGRGYVLRRLLRRAVRHGKLLGVNEPFLYRAVDLVVREYGDF
                     YPELSQGQEYIKQVVQQEEDRFHETLEQGMKILTELTNQLGTKGTLAGAEAFKLYDTY
                     GFPIDLTKEILAEQNLQLDEAGFKEALEAQRERARSARGQHGYGTEVTSYHHLRGLES
                     EFVGYEQLHTESKVLVIFKNGQPVTAAAAGDEVEVVLSQTPFYGERGGQVGDSGLLSS
                     PQVMLEVKAAIMAPDGQVVHQGTITSGQLQMGDSVQAVVQHEKRGAICRNHTATHLLH
                     QALREILGSQVQQAGSLVDKDRLRFDFNHLSSLTPEQLASVEARVNEKIWENLPVEVT
                     LTSLDEAREMGATALFEDKYGDQVRVVRVADYSMELCGGTHVTNAGQIGLFKIISEAG
                     IGAGLRRIEAVTGPGAYQWFNQRSQILEQAAAQLKSTATQVPEKISGLHIELKEIQRE
                     NQRLQLKLAGMEVDGLLANVSRASGVPVLSARVSAGNMETLREMADRLKSKLDSGIIV
                     LGAAAEGKVLLVGAVTADLVKQGYHAGKLIGAVAKLTGGGGGGRPDMAQAGGKEPAQL
                     DLALAKVNEFVQKQQK"
     gene            19161..19430
                     /locus_tag="VFC74_04735"
     CDS             19161..19430
                     /locus_tag="VFC74_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004037635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HZK24676.1"
                     /translation="MESFDQTMKFKRTPDDETYQARDVFAVVYSALKEKGYNPINQIV
                     GYLLSGDPAYITSHQNARVLIRKLERDELLEELVGSYLARLDHEA"
     gene            19433..20380
                     /locus_tag="VFC74_04740"
     CDS             19433..20380
                     /locus_tag="VFC74_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK24677.1"
                     /translation="MEYRTLGKTAIRVSRLCFGTLTLGPLQSNLSLQEGVRLLEIALD
                     LGITFFDTADLYGTYPYLREAIRHRPQQPVISSRSYDYTREGMQATLQRALTELAVPY
                     IDIFMLHEQESPLTLAGHRPALEYLFEAKARGLVRAVGFSTHRIAAVQAALTMPEIDV
                     VHPLINRLGVGIQDGTAEEMLSVLTQLHQQGVGIFAMKALGGGHLIPQSEAALQYVLS
                     QTCLDAIAVGMVNEEEIRCNCALFAGETVSSQVMEHLQRKKRQLVVQEWCVGCGTCVK
                     HCPQGALHVENERVVVAAETCLLCGYCGAHCPDFCLKIY"
     gene            20394..20822
                     /gene="ruvX"
                     /locus_tag="VFC74_04745"
     CDS             20394..20822
                     /gene="ruvX"
                     /locus_tag="VFC74_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015615576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HZK24678.1"
                     /translation="MRILGLDVGDRTIGVAVSDALGWTAQGIEVIRRGTWEADVQRLR
                     EICVEYDVTLIVVGYPKNMNGTIGPRAELSAQFAEQISQALSLPVKLWDERLTTMEAE
                     RMLIAADVSRAKRRKVIDKMAAVLILQNYLAAQTVTKTDK"
     gene            20882..21190
                     /locus_tag="VFC74_04750"
     CDS             20882..21190
                     /locus_tag="VFC74_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HZK24679.1"
                     /translation="MTDKMQELQDEVVTLIDEEGQEHQFMVLDIIMVDDSEYAIMIPA
                     LEEEDTEEEEQEAIIFRIDENDGQQSLTVVDDDDEWDAVAAAWEELAAMEDDEFDDEE
                     "
     gene            21220..22260
                     /gene="mltG"
                     /locus_tag="VFC74_04755"
     CDS             21220..22260
                     /gene="mltG"
                     /locus_tag="VFC74_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HZK24680.1"
                     /translation="MSKGKARRLLAGILIVLVVAVGLFAIQLNTWLKPVPAMAQEAVM
                     VAIPSGSSSMRVANILAENKLIRNAAVFRYYAKFRGMDQGLQAGNYIFSFGMTMDEIL
                     RHLLEGNVYRPTVTVTIPEGYTVEQIAQRLEATGLVDAEVFLTLVASTEPELGKVVTG
                     QRYAMEGYLFPDTYEFDINATPQQILQRLQARLGEVLTPARRERAAALDLDIHEVLTL
                     ASLVEREVQAAAERDMVAGVMFNRLQKKMPLQLCASVIYALGVHKTEISTKDTQVESP
                     YNTYKYSGLPPGPIASPGESAIDAVLNPADVDYLYYVVKGDGSGEHYFGLTFAEHQAN
                     IAKSQQNEKKRK"
     gene            22269..23489
                     /locus_tag="VFC74_04760"
     CDS             22269..23489
                     /locus_tag="VFC74_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HZK24681.1"
                     /translation="MQKIELLAPAGDLEKLKMAVLYGADAVYLAGKSFGMRAFAGNFG
                     EEEMREGITFAHQHGAKVYVTVNIFANNQDIAELPPYLELLAALKVDALIIADPGVLA
                     VVRQTVPSLPCHLSTQANTTNWRSAKFWEDAGFSRVVLARELTLAEITEISQQTNLEL
                     EVFVQGAMCWAYSGRCYLSQHLAGRSANRGECAQACRWKYNLVQERQPGEYMPIEEDE
                     RGIYILNARDLCLLDQLGELAQAGVNSFKIEGRMKSVYYVATVTRVYRQALDAYLADP
                     ENYTIDPAWHEELHKISHRPYTTGFIDGNPGESGQVFDSSAQVSSHEFVGVVRAYDEA
                     AGRAVVEMRNRFAAGESLEVVGPHTSSREFTVSALKNSEDQPIAEAIRVQEHVSFSIP
                     FPVEEYSLVRRQKT"
     gene            23707..25263
                     /locus_tag="VFC74_04765"
     CDS             23707..25263
                     /locus_tag="VFC74_04765"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HZK24682.1"
                     /translation="MRRESRQKRIVVMIGCLLLAVSALVGRLFYIQIIKGRDYAERAV
                     AQRSAAYVYDSGRGQILDRNYQSLHAIRTKDSSRLQAELVAYNIPRDLLTGLEIEEEI
                     RYHAGSLASHVTGYIKRPRNPMQAQNGLAGLERAFNNELWGSPAAIGVIIDANREMVA
                     GLGINEWQYEHFRRPYHVVTTIDRQLQGIVETVGGSLIKKGAVILLEPKTGDIVTMAS
                     FPRLLVEKLYSGVEQQELADMENGNAYLNRSLMQYPTGSVFKVMLAAAAIEEQLPEPE
                     GLFLCDGSYEVGNRTISCYNGTSHGVVDLHAALAVSCNGYFVNLAERLGSEKLLAMVR
                     RFKLGQATGVPLGGEMPGKIPTAAELPYPGDLANTAIGQGLVAATPLQLARMMAIIVN
                     DGRDIYPRLVSQIIDKNGNPVKNYPVQYGTRVVSPLITRQLKKMLTTVVNNGSANSAA
                     SELYQAAGKSGTAETSKTGVSHSWFAGYVDLNGRILVAVAFLEEWRPKEPTASSVLKQ
                     IMEAIAIAQS"
     gene            25399..26763
                     /locus_tag="VFC74_04770"
     CDS             25399..26763
                     /locus_tag="VFC74_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011098884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HZK24683.1"
                     /translation="MVSEKDMHEGNIFKLILNFSGPAITGMLVMSVYNIVDRIYIGRG
                     VGTLGIAGIAIGHPLMMIKGAMAMLVAIGSSAMISINLGQKKKEAAEKILGHGFTLLV
                     VFAIFASGVSLVFLTPLMRLFGASENVLPYATDYMRVILIGNVFQMIGYGANNFIRAE
                     GNPRTAMFSSIIGAGTNIILDPILIFVFEMGVTGAAVATIISQAASAAWVLHYYLSGK
                     SYLKIRKINLRPELPLSLKMMSLGTSSFVRQVSNSMSIVLLNNSLQHYGGDMAVSAMG
                     VIFSIRTLQMMPVLGISQGVQPIIGYNYGAQQLGRVKKAFKLAVLLATAATVLNFAAI
                     MLFPEFFFRLFTTDAELIAVGAAGVRRYMLFLPILGYQVLGANYFQAVGKPKQAIFLN
                     LSRQAIFLIPALLFLPRYFGLSGTWSAQPTADALSVLVTTALVWYEMRRLTQQDKQLQ
                     QLTN"
     gene            26794..27006
                     /locus_tag="VFC74_04775"
     CDS             26794..27006
                     /locus_tag="VFC74_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24684.1"
                     /translation="MIDSTILYLMAAPIYLAVLIVVIKKQVPIQKQVLIALFFIYLIG
                     FFSVTLFPLPVQNIVSPLSRQRFSSF"
BASE COUNT         7387 a         5629 c         6806 g         7824 t
ORIGIN      
        1 tttttatata gtggtataac aaattgtttt acccgctaga taaaaggatg tgatcagctc
       61 atgaaatcta tcgtgattac ttgtgcaact attcgtaaag aaatagacca agctataagt
      121 aaaacaggtt ttactagtcc agttttatac ttggaagcag gactgcataa ctatcctaaa
      181 aaactgcatc ttgcgttgca ggagcttctt aaccgtgttg ctaatgtcga ccaggtcctg
      241 ctggtaatgg gctactgcgg caatgcggtg ttagatttag aagcaaggga ttatcgcttg
      301 ctaattcctc gtgccgatga ctgtctcaca atgctgcttg gctcgcagaa aagacggctc
      361 gaagtgcaaa atgaaaaaca aacctacttt ttttctcctg ggtggctaga tcattttgaa
      421 gacctggaaa aagacatggt ccagggcaga gcacgcatgg aacaaaggta tggtaaagaa
      481 aaggcgagaa aactgttccg agaaatgttt aaacactatc ggcgtgccgg tttaattgat
      541 accggtactt tcgagcttga cgaactgctg gtaaaagcac agcagcgagc cgcagaaatg
      601 gagttagatt gtgaagtgat tcccggcacc atgagttatt tagagaaatt tcttgccgga
      661 ccttgggaga agaaagattt tattattctg cagcctgggg agaaagtaca ttattcgcat
      721 ttgtttggta atgaggcgta aagtttagac gtcatcggta aattagccta gcagagaagg
      781 ggtggcgatt agatgaggca ggatttgcca gcagaagaga tgcgccttat tttgtggacc
      841 attataaact cggttcagga tgccatttct gtggttgatg agcacggcaa aggtatttta
      901 tttaatcatg cctatttgca agcaacaggt ctgcaggagg cggatgtttt aaataaacct
      961 gcagaagtag atattgtgga aggtgaaagc gttcacttaa aggttttaga aacagggaaa
     1021 cctatcagag gagtgcccat gaaagttgga cgctataaac gtcatgtcat ggtctcctgc
     1081 gctcctcttt acattgataa taagctgcgc ggcagtgtgg ggattattca tgatgtttca
     1141 gaagtgaggg cgctgtcagc tgaattacag cgggcgaagc aaaaagtgcg ccagctggaa
     1201 acaaaatata catttgacga cattattggt gaaagcactg tgcttaaaca ggcaattcag
     1261 ttagccaaag tagcggccgc aactccagcc acagttttat tacgtggtgc aggaggaaca
     1321 gggaaagaac tatttgcaca cgctatccat cacgctagtc cccgtcaggg tgagcagttt
     1381 attcgggtta attgcagcgc tttcccagaa agtttgttag agagtgagct tttcggctac
     1441 agtgatggtg ctttcacggg tgctcgtcga cagggtaaaa agggtatctt tgaagaagca
     1501 gatggcggca caatttttct tgatgaaatt ggcaccatgc cgcttagtct gcaagcaaaa
     1561 atcttacgcg ttttacaaga aggggaaatc gtgcgcattg gtgaggctcg tcccttaaca
     1621 gtagatgtac gcgtcattgc cgcgaccaat gctgatttag aaaaactgat tatggaaaat
     1681 aagtttcgtg aagatcttta ctatcgactt aatgttttcc ccatttatat accgccttta
     1741 gttgagagaa aagcagacat tcccctgctc gctcaatatt atttgcatgc gttgggtgag
     1801 gattataatc ggccagatac cgagctatct tcaacggctg ccaagatgct gcaagaatat
     1861 cattggcctg gcaatgtccg tgagctgaaa aatgtgatgg ctcgtgcctt gattaacctc
     1921 aataaagacg aaaagcttgt gaaaacgaga cacttaatgt tttctttgag tacttcacag
     1981 caaatcagta gtgggcgtga tgtccaagtt tatgacgggg aaacgctagc ccaacaacat
     2041 gccaggtggg aaaaaaatat attactagaa acactgcaca gtaccaaggg taataaaaca
     2101 gaagcagcgc gccagcttgg tatttctgta cgcagcctct ataacaagct ggagaagtat
     2161 ggtgtttaat gctagctcta cacgcacttt ggcatgactc ttgcaataat ataagggtgt
     2221 aaggcaagaa caaaagctta aaaagctgct tttaaaggat tgctgcccta catgctgaaa
     2281 tatattgcct gcaaaaaact gcagagtgtg aaaaaaattg cagatattga cgaataagag
     2341 gatttaagca ctcccccagg tgcgcaaatc cgcagtatac ccctactaac caatttttcc
     2401 tcattaaaaa tcaaaagccc gcctctttgg cgggtttttg attaactctt tctaaattta
     2461 aagggatttt ctctcctgct acagaattta acacgaagcg gtaagccgcc taataataat
     2521 tattagcaga tagacggtga ggtaagtgat gcataaaaag aatatcgttt taattgggat
     2581 gccgggggcg ggtaaaagta cagtcggcgt gttagcggcc aaagtattag ggatggcctt
     2641 tgtggataca gacctgctga ttcaggagca ggaacaacgc ttactgcagg agattatcaa
     2701 tcagcagggg attaaagaat tcctaaaaat cgaagaagaa gtgcttttcg gcctacaggt
     2761 agaaaatagt gtcattgcca ctggcggcag tgctatttat agtctacctg ccattaaaca
     2821 tctgcaaaac tctggtaaac taatttattt aaaaataagt ctgcctgagt tggaggaacg
     2881 tctgggaaat atggcccagc gtggtgtggt aatgggcaag ggcgaaacac ttgcagattt
     2941 atatgcggcc cgaacaccat tatatgagca agctgctgac cgtattgtgg agtgttcaaa
     3001 tttaactacc gaagaagtaa ttacgcgtat tattgccaat ttgcataatt agcgcttgtt
     3061 gaataagtca tgttggcttc tcatattcag taagcattaa gtgctggctt aatgacttga
     3121 agacgaaagg ggaaaataaa tctttcttta tcctgattcc acctctggct gtgattatga
     3181 tctttggctt attgattgtt aatatcgttt tagccattgg cattttacgt ttcatgaaaa
     3241 aatgcgaaga gcgtatttag atgagtctaa ttggcatttc ctggtactag gtttaaatat
     3301 ggcgttgccg tacaaaggta agtgtgctat acttggaact tgggtctaaa aacccagggt
     3361 tttttctttt ctccaacatt aaatatgcaa ttaaagtatt ctataccaca atgcgcagat
     3421 tatgggaggg tagcagaatt tattacggta aaattaatag aagtacgcaa gcaaacttat
     3481 taagttccag caaaatttat tgaaaaggat gaaacccaga tgcaacagga aaatagtcat
     3541 gaattaggac agacaaacat caagaaatta ctttttaagt attcctcccc cgctattatt
     3601 gccatgcttt ttaactcttt atataacatg gttgatacaa ttttcgtcgg acgaggagca
     3661 ggtacactag caattgctgg acttgctatc tcttttccta ttcagatgct ggtcatggct
     3721 atcgcccaaa cagtcggtat tggcgcggct tctatcatct ctcgcagcct aggagcagga
     3781 gacaaaaaaa gagctgcgca agcagccggt aatgctttta ctatagtctc gattctgagc
     3841 attgccttca tggttttcgg cctcacttat ttaacaccgc tgctgcgcct atttggtgca
     3901 acagaggcta ttttgcccta tgctagagag tatatgtcag tcattttagc tggcagcgtc
     3961 ttcttcagtc tggcagtgtg cagcaataat attgtcagag ctgaaggttt tgcgaaagta
     4021 gcaatgtata gtatgctaat tggagcaatt ggcaatatca ttttagaccc catttttatt
     4081 tttacttttg atatgggaat tcgtggtgcg gccttggcca ctattattgc ccaatttggc
     4141 tcgtttttgt atttagctgt cttcttcttg cgtggcagta gttcagtaaa gattagccgt
     4201 caagggctaa aaattaattg gcagctagtg cccgaaatct ttgctgttgg tgcttcttcg
     4261 tttgtgagaa cggtggcggg aagcttattt tccattgttg tgaacaactc tattgcttat
     4321 tatggtgctg atatccatat tgcagtattg ggtgtggcta accgcttaat tacttttact
     4381 atgatgccga ttatgggtat tattcaaggc ttacagccta ttatcggctt taactacggg
     4441 gctaagaaat ttgcgagagt aaaagaaagc ctgcagctag ccattaaggt tgcttcggcg
     4501 tttgcactag tgggctatct tggcatgatg ctttttacag aacagtttgt gcgtattttt
     4561 aataatgatc ccgcactggt cgtagaggct gtccccatta tccgtgcgat ggccctacta
     4621 ataccgctca ttggttttca aatggtaggc tccagtttat tccaagctat tggtcgggct
     4681 gcaccagcac tatttctttc catgtcccga caactgctta ttttgattcc tttagtctta
     4741 attctgccct tatctatggg tctgattggc atcttttatg ctttcccgtt gtctgattta
     4801 ctttcaacga tcattaccag cttatggtta agacgagaaa tgaggattat gtccaaagaa
     4861 aaaggtgatg gaccagctaa agctgatgat cacctagaaa aagtaagtta aaatggtgtc
     4921 actctagagc agcttgctgc tcttttttta ttgattaaaa accttttgca gaatttgcgg
     4981 gtcacattct taatttttta tgtatagaat gtacggaaag gtgaaagggg gcccaagatg
     5041 accgttgagg aagttcttgc cgatttattc gctcagctag atcaagcacc gccagagctg
     5101 atggccatgg atgctgtctt cgagttaaat atattggggg cgcaagctgt aacctatcag
     5161 ttaaaaataa aaaatcagca aatagagtgg gcaaagggta ccccgttttt gccaagctgt
     5221 attttttatc tctctgatac tgattttctc aagatagcgg tgggagaatt aaacaccaaa
     5281 ctagctatta tgacggggcg gataaaaata gaaggggatt atggtcaagc cttacaatta
     5341 agcgcgatca tcaaaaaaca ctacaaacca tactagtcat gctgaccatt taattgtcgc
     5401 cgaagagtaa gcatagccac ccgcttgcaa aagcgagtgg ctattaattg gttgatttta
     5461 caaactacaa gttattaagt tgaattagtt cctgcacggt aattttatag cggcgggaaa
     5521 tagcgtaaag agtatcccct ggctgcacag tataggcgct cgttgcttct gtggctgtgg
     5581 gcggaagcat ttcattgact gttactaggc gatagccttg gtcttgcagc cctgtgatga
     5641 tgcggggcag agctgcgaca gtttgataac caccaagatg cattaagata ataccgttgg
     5701 gggaggcatt atttagtact cgtgctacca gataatctgt agttacttgt tgtccgcgta
     5761 tttctgccgc ccagtcgtgg ctatctacag tccaggtcac ggtgtaggga tagccagctt
     5821 ctcccactaa ctgcagtaaa cgctcgtggt attcgccgta tggcgggcga aaaaggcggg
     5881 gctgactacc ggtaatttcc ttgatgatgc tcgtagattt ctgtaattct ttgcgtactt
     5941 catcgtctgt catcttgatt aagtgcccat gggttaggga gtggttctct aatcggtggc
     6001 ctcgcctgtt tatttcccgt gccaggtctg gatgcgcttc tgcccagagg gcacgaagaa
     6061 aaaaggtaga cttaatacca tagtcgtcta aaacatctaa aatgtcgttg gtctggtcaa
     6121 acaaccagcc agcatcaaaa gttaatgcta tctgtggctg ttgactagat attacccctt
     6181 gagtaataac ttgagacact gtttgcttgg tgccactaaa agggagaaga agcttttgcc
     6241 ccacgtagat aatgggagtt gtctctgcat cgtgcggaat gtctgctggt tcttcctgca
     6301 tttgatctgg ctcttgtttc tcatctgttt tttctggtat tggctgctca atatcctcat
     6361 ctatttcttc tggtattggc tgctcaatat cttcatctgt attgggcagt ttcccctccc
     6421 caggggcggg aagctcttca gtaaaccaac tgtgccaggc agcaagaaac tttacttgta
     6481 ctccctctac ggctaaaatt gccgtcaaaa ataggagtag cagggcaaca acaataatgc
     6541 ccccttgctt cttccttaat ttcttctgct cctcagtcat gccgaaccct cctgctttag
     6601 atctgaagaa tgttgtaaat aagaactttc ctaatcgaca gtataagcga taaaagctaa
     6661 gtattcctta ttattcgatt taatttctat taattctttt taacagctgt tactgtccaa
     6721 ggcaaaaaaa aactgccccc tgcggggaca gctttttgga gattgctgat caactagtca
     6781 attaaaccgt tggcttttaa atgttcacgg gctacatctt ctggctccat ctcgtccact
     6841 gaaacttttt tgtttagttc ctgcatctgc tcagtggtaa taagattttc aattccctgc
     6901 agagcttctt cgagttcagg atgctcttcc agcacttccg tgcgtaccat taaaatacca
     6961 tagtaaggtg ggttaaagcc cttgtcgtct tcgagaattt ttaaattttc gaccacaagc
     7021 tcaccatcag tagagtaggt attgatagca tcaacttcat cattgcgtat cgccatatac
     7081 atcaaggagc ggttcatttc tacagtttct ttaaaagaaa agccgtatat ctctgtgtac
     7141 tctttgtaac cctgacccgg gtaattcaac caagatgggg tgaccgctaa actcatttct
     7201 ttagcaaagg gggcaagatc actctgtttg ttaatgttat tttcatcagc ccatttacga
     7261 ggtacagcta cacagaaggt attgttatag ccgtaagagg ggaagacagt taggccatga
     7321 tctttgggga ggttttcatg gacaaattcc catacttttt gtggatcacg gtattcttca
     7381 gttaagccct gctcgtatag acctaaaaat agggtgcccg taaagttatt acacatatct
     7441 aactgaccac tgacagtagc ttcatgagct gccattgagg agccaagatt gcgtatatgc
     7501 tcaaccttat ggggtgttct atcctcaatg agaattttag ttagttctgc tagaatctcc
     7561 acttcagcat aaccctgtga gccaattttt aaggtaacgc ctttttcatc aggaggtgtg
     7621 tcaccagatg tgtctccgtt tccacaaccg gttaggatgc ccgccaaggc taaagttaga
     7681 accagcaaga tagagcacaa tcttcttgtg ttgatcattg gttttcgcac ttcctttcta
     7741 tttttctgca cagatgcatt ttgctccgta caattgcccc taagttgtcc aggtgagaaa
     7801 ctttagtaaa agtattcaga attgtatatg cgtataatct atcttaacgg aaagtaacaa
     7861 ttctgtcaag ttggcagcaa tttgtaaaaa cctgttattg cttaagctgt ctgccattta
     7921 ctaggtgagt aatgttttaa aggcaggaaa aatgtagttt gataccgaat tattacaagg
     7981 ttaagtgatg ctgtactatc gcaagacata aatattggta ggattagata atcaaagggg
     8041 gagaaaggtg aacatttcag aacttttggg taaagatgcc caatatttgt tggagcatca
     8101 atgtgatact atagagcgag caagactgca tctgcccggc cctgattttg tgggcagagt
     8161 agttgccatg acggacagac cggtaccggt tttgcgcagt ctgcaaacta tgtttaacca
     8221 cggacgcctg tcggagaccg gttatctatc aattttgcct gtagaccaag gcattgagca
     8281 ttcaggaggt gcttcttttg cacccaaccc tgcttatttt gatcccgagc aaattgtgaa
     8341 gttggccata gaaggtggct gcaatgctgt agcctctact ttaggcgtgc tcggtgcagt
     8401 atcccggcgt tatgcccata aaattccctt tattatgaag ttaaatcata atgaattgtt
     8461 atcgtaccct aatacatacg atcaagtaat gtttgctgat gttaagcagg cttgggcgat
     8521 gggtgctgtg ggtgtggggg caactattta ttatggttcg gagcaatctc gccgccagat
     8581 tcaagaagtg acggcagcat tccgtctagc acatgaattg gggatgttta ctgttttatg
     8641 gtgttactta agaaacggct ctttcaagat tgatggtgta gattaccatt ctgcagccga
     8701 cttaacaggg caggctaatc acctcggggt aactttagag gcggatatta ttaaacaaaa
     8761 actaccggaa gtaaatggtg gttatccggc tgttagcaag caggcgggta aaacatttgg
     8821 caagatacac gataaagtat atagtgattt aactacagat catcccattg atctggcacg
     8881 ttaccaagtg gcaaactgct atttgggtcg ggcaggccta attaactctg gtggagcctc
     8941 tggtaaaaac gatattgtgg atgctgtgcg tactgctgtg attaataaac gtgcaggcgg
     9001 catgggctta atttgcggac gcaaggcttt ccaaaaaccg atggccgacg gtgtcaagct
     9061 gctcaatgcc attcaagacg tttatctgga ggcaaaaata accatcgctt aaatttaaga
     9121 gccggcgtca agccggctct taaatttctc tctcgctgtc agtgctgtta gttaagttaa
     9181 gtgaattatt caagcctgac cccacaaccc tgttaacccc aggggtgggc ataggttgac
     9241 aggataaaaa tagtcgtgct ataatcctgc taacatcttg gcaggaggca gtgctaatga
     9301 caagaaaatt cgctactcgt gacttagtat taatagcttt atttgccgcc ttaacctcac
     9361 ttttagctta tctgattatt ccgcttcctg gtggtttccc gccgctgacc ggccaatctt
     9421 tagcggtaat gttagcgggt ctgctgcttg gagcgcgcaa tggggccatg agtcagcttg
     9481 tttatttatt gctgggcgca gccgggctgc cggtgtttgc tggtggcacc gctggtgtgg
     9541 gcgtcatcgt cagcccgaca ggcggtttta tctggggttt tgtcgtagga gcctgggtaa
     9601 ttggtaaact aactgaagat cagaagaaac ttacttggtt acgtttagtt agtgcggcag
     9661 tttgcggtgg tgtttttgct gtctatgtgc cgggagtttt gcaaatggcg aatgttctcc
     9721 agattacacc aagtagagct ctggtggcca tgttgcccta tctgcctggt gatttactga
     9781 aagtttttat cgcggcagca cttgcccgca aaactatgcc gcagttacaa taaaggtatc
     9841 agttctctgc caggaaagga tgacaagaat gacacgtagt tttttgcaaa cagatatata
     9901 tggtattacg tgtgaggcta tgtctgccgg tcgtagtaat ctggagactg tgcgcgagct
     9961 tttacaggca ggtgtgcaaa ttatccagta ccgggaaaaa gagaaaacaa agcgtgaaaa
    10021 attcgcccag tgtttagccc tcagcaaaat gtgtaaaaaa gcaggcgcac tgtttattgt
    10081 caacgatgac gtggacattg ctttggccgc ccaggctgat ggcgtgcatg taggacaaga
    10141 tgacctgcca gcagatattg tacgtgccct agtaggctca aaagtgattg ttggtgtttc
    10201 tgccggcaac actaaagaag ccgctgcagc tgtggcgtgt ggtgctgatt atctcggcgt
    10261 cggccctatc tttgctacag gaacaaaact agatgccggt gcgcctattg gtttagaatt
    10321 tttagcttat cttaaagcaa gatatacact gcctttggtg gcaattggcg gaattaaccc
    10381 tgagaatata acacaagttt ttcaaagtgg tgcagattct gctgctatga tttcggcctt
    10441 agtctcatcc tcggagctcg gcgcgacggt gagggcaacc aggcagcgtc ttaaagaaca
    10501 cggctttgct gggtaaactt ccaagctgag ttgaataata ggatagtcaa cactatacca
    10561 aagctaacgg aaatattgcg gatagcggct acgctgatat aagtcgtatc tacgcggggg
    10621 tagatcccca ccacatagtc taaataatcg ttaaagaaat accaagcagc aatggctgtt
    10681 gcttgggcat agttgagggg cagccgataa taaaaccaaa agaggctcgc ctcaagcgcc
    10741 atccccagat gtccgctcca caataaaata tttgcgaaat gacggtaacc actgagctga
    10801 taaacagtaa tgatcaccac tgtccatagg ccgtgtttaa tgaggccaaa gtaagcgaat
    10861 ccctccaaaa agtagtgtcg ccggcctctc ctcagccgta gtaagaccag aaagaaatat
    10921 agcaccgcca ggggagaatt aggtacaaat ggccagaggt aaggtggtgt cgtgacaaac
    10981 tgaggctgat accaccagaa accccagaca atgccgatga aattaattac tagcaggctc
    11041 acaaaaaaca atttgttgtt aaagacagct tttaaatact gacgcatcag ccactcctta
    11101 atgaactttt atctgatagt atgcgcaaaa gacggcaaaa aaaaatcctg cacacgatcc
    11161 ctcacgctcg agtactggcc gtctgtgccg ctttgttaat gacaagtaac tgcaagtaac
    11221 ttaattgtgc cagtaaaaaa agactggatt tgctccagtc tttaagcttc atctgctttg
    11281 ttttccttga ttaaatcaaa ttccgcgaag acacgcagca ctgctttaac ggaaagcact
    11341 aacatttctc gttcttcggc agtgagccgg gcggccaaat ggttaaagcg tttgttgcgc
    11401 cgctcaaaaa ctaaactggc taattgcgta ccatatgagg ttaaataaac tctaactaca
    11461 cgccgatctc ggtcatcgcc ttgacgttta actaaattta atttaactaa gcggtccact
    11521 aaacgtgtgg ctgcactttc cgccaggtag atttcggcgc tcaattctcc catggtgagg
    11581 ccgtcttgca taaatagaat caataaggca ttaatttgcc ccggcgtgat gcccagccca
    11641 gttaattcct tactttcttc cgtttctaag tagtgcgtta gtttaggaaa gatctcctga
    11701 aatacttcag taaagtatga ggtattttca aacaaggaat ctgcctcctc catatttctt
    11761 tttgatagtt ttgattataa cacttcaagt caaaaataga taggaaaagt agttagcatg
    11821 acaatatttg cagctctagc tgcaaataat ttaacaaata gcggaaatat taagctgtga
    11881 ttgataaagg tgcagtaaaa aaagtaagtg ggcggaaaaa atcccgtact ttggtatact
    11941 atttagcata gaggagggag cctggtgtta gttattggct taacaggcgg tattgctaca
    12001 ggtaaatcaa cggttgcaga cttatttgct aaattgggtg cctatcggat tgatactgat
    12061 catttggcgc gcttgattgt ggtgccggga aaaccagctt ggcaagcaat tattcagtat
    12121 tttggtgaag aaattctgct ccccgatggt caaataaaca gaagcaaact agggaagtta
    12181 gtttttagcg acgccaagaa gcgtcaggtc ttagaaaata ttacacatcc ggccatccgt
    12241 gagttaatgc aaattaagtt aagtgaagcg cgggctaatg gtatttgtct tgcgctagtg
    12301 gaagttcccc agctgtttga aaccgacttt caccaagatg tcgaccaaac aattgtcgtg
    12361 actgccgggg aagaacagca gttagttcga ctcgctgtcc gcgatgggct agtgggtgaa
    12421 gatgcacggc agcgactggc agcacaaatg ccgctctcgg cgaaagcagc tcgcgcagac
    12481 tatgtcattg tcaacaatgg ggatctggcg gagacagaaa ggcaagtcaa ggtgctttgg
    12541 cagctgttgc agcgggagtg tgtcaatgac tgaattaatt cgtgggtcgc ggctgctctt
    12601 catgctcatc attattgttt tgctggttgt tttttttaga tctccttact ttcgcagatt
    12661 atcttatccc tatcaccacc ggaaaataat cgaaaagaat gctttggagt ttcgtgttga
    12721 tccgctgctg gtagcggcag tgatacatac tgaaagtaag tttgacgtac gtgccgtctc
    12781 gcctaaaggt gccttgggct tgatgcaaat tatgccgcct acggctgcct ggattgcaca
    12841 aaaactcaag ctggggtcgc tcacgaagga acaaattttg cagccagaaa caaatatccg
    12901 ccttggtgcc tggtatttag ctaacctgac gcaagagttt aatggtcagc ttgatctggt
    12961 aatcactgcg tataatggtg gccctggtca agtaaaccgc tggttagcca gcggggtatg
    13021 gtccggtcgt tatgctgacc gtgcggaaat ccccttttcc gaaacaagag agttcctgga
    13081 aagggtacgc accacttatc tacagtatca agaactttat cgttaagagt aaatatcaag
    13141 tcgcacttag caggttttta tctaaacagg attaaaagct ggcactagga gcctgacagc
    13201 gcatacttct tcgttctgct tgccgctgag taccagtcgt gttaagatgg gaagggtaac
    13261 aagtgcctaa tgtattaacc gggagggatt gccatggccg gccattcgaa atgggccaat
    13321 attaaacaca aaaaagcgcg tgctgatgcg caaaaaggaa agagtttcac caagatatct
    13381 aaagagatta tggctgctgt acgacaaggc ggcagtgatc ctaatggaaa ctttcgctta
    13441 cgcctagctt tgcaaaaagc tcgttctgtc aacatgccca atgataatat tactcgcgcg
    13501 gtgcaaaaag gcgctggtga gctggacggg gttaattacg aagaaattgt ttacgagggt
    13561 tatggcccgg ccgggacagc tgtactgctg gatattctta ccgataaccg caaccgtact
    13621 gtcggggaaa tacgtcatat tttttctcgc cacgccggta gtctgggcgc cacaggctgc
    13681 gtagcctgga tgtttaaccg caaaggttat ctggcagtgg cagcggacgg tattgatgaa
    13741 gatgacttga tgctgcaggc tttagatgca ggagcagcag actttgaagc cgatgaggat
    13801 ggctatgaga tttatacagc accggacgat tttgaagccg ttaaagaaaa actggaagta
    13861 gcgggtattg attttacgga agcagaaatt acgatgattc cagaaaatac ggtagtagta
    13921 accgacctgg aagatgcccg taaggccatg atcttgattg aacttttaga ggaccatgat
    13981 gatgtgcaaa atgcttatac taatttagat attcctgcag aggtagccca agctttagag
    14041 aatgaatagc aaatagtata aaccccctga tcacgggaaa ttcttaaagt aagaacgtga
    14101 aagggggttt tcttgtgtta aaaaagaagc agccgggaat ttataccatt agtatttttt
    14161 cgcttggttt attactcggt ttagtgctgg gtaaatttga tttgctgcct ggtgccacag
    14221 cggcaatgcc tcaggccgcc gaaggggcca ttctttttgg tgccgcggat gccagtcagc
    14281 caggctctgc gacagcggta gaatatcagg gttatcttgg tgtctacgaa gatcatcttg
    14341 ccatctatga cggcctgccg ccgcatggta cgctgcagca tgtaattgcg gattatgaag
    14401 taagagatga tgtgcgcccg cttttagaag ccggaattcc gtttactgat acatatgact
    14461 tactgcgtct cttagaaaac tatactagct gaatcagata aaagcagtgg gggtatacta
    14521 tttgtcatac ttttgggggt gtcacggtgc gtttttcccg ggagttgagc ggtctagccg
    14581 taattcatta cactagtggt cgtgagttag gtaaagtcaa agaatggttg ctggacgagt
    14641 atggtgaaac ggtagtggct ctagtgatcg agggcagcgg ctggctgcct caacgtcgca
    14701 tctaccctta tcaagatatt tttagtatag gtaatgatgt tgtcttagta agcacggaag
    14761 ggctgctggc agcgggagat ccaccgcagc tggagtcaca ggcgactttc cgggtgttag
    14821 gcaaacgcat ggtatcgggg gatggtaatg aattagggat tgttgaagat gtgctgtttg
    14881 aagaagaatc cggtcatgtt acaggctggc gtctctctgc tggactaatc gatgatctct
    14941 tgcaaggtcg tcctgtgcta gaggaatcct tgcagcttca tattggggaa gatgctctga
    15001 ttattcgcga ttaaagaaag aggtgtggcc atgaaatgtc ctttatgtac aagtattgat
    15061 atcggcaaag tagggacagg tcaatactat tgctggaatt gtctgcttga atttactatg
    15121 accagcaaga atgagtttac agcgtattat gttgatgagg aaggtacact tgtcaattta
    15181 agtgaaatgg tgcaaaacaa tcaactgatg gaggaaactg tcttaggtta aaaagaatca
    15241 catttttttc cccgcatata gataaataaa cagacggggg ggggagacat ttgctgacaa
    15301 caaaaaacta ccgcagtact tattaccgtt atgtcgtgct tggcatcact gtcgctcttg
    15361 ttttagcttt actgtactgg ctgtcgcgta caggagcagt gctggccgca gttcttgtgc
    15421 cttttctctt ggcgattgtt ttagcgtaca ttctaaatcc tgtagtggaa ttcctggaaa
    15481 aacgccgggt cccgcgcact ctgggaattt tacttattta tgccgtcttt tttcttgcgg
    15541 gtaccgtctt tagtttgaca gtgattccta ccttaattac tgaactgcaa aaacttggcg
    15601 agatgattcc gcagtataca gagcagtttc aagcatttct gctgcatctg cactctgatt
    15661 atcaacgaat taatttgcca gaaagccttc gtttggccct ggatcaaaat ctttttgccc
    15721 tgcaggatgg tattcaggcg atcctcgaac gagtgacagg cactgtgtta agcctctttt
    15781 ccaatctgat gacgattttg attattcctc ttgttgttta ctatatctta cgggatatgg
    15841 ataatctcaa gcgttctgca gttttattat ttcccaaaaa atatcgtaac tggatcatct
    15901 ccatgggtag tgaaatggat cgtaccttgg gagcatattt tcgtggtatg ctgattattt
    15961 gttttttagt gggcttttta acctatctcg gtttaaaggt gataggtctg gattttgctc
    16021 ttatccttgg tgttatcgcc ggcctgacca acattattcc ctactttggg ccgatcattg
    16081 gtgccgtgcc cgcaatattg ctcgccttgt tatcttcttc cggtttagcc gtcagagtga
    16141 ttgtcgtgta tacattggta cagcagttag agagtcaagt tatttcgccg caaatactgg
    16201 ggcgcagttt aggattacat cccttgttcg tcatctttgt gctcatttta ggaggcagat
    16261 tcttgggttt accaggttta atctttgctg tgccctttac ggctattgcc cggatatttt
    16321 taaagcacgc catcgatttg gctgccaacc gtacctagct atgcagctgt ggtaacagct
    16381 aaaacgatat ctacactagt ctactgccca ttttattgac acaattcgtc ttcctcttat
    16441 ataatggaat ttgatatgat gcagggaggg tttggcatgc tgtccaaaga tatccggtca
    16501 aagtttttaa attttttcca aagtaaagat catctggttt tacccagttt tccactaatt
    16561 ccgcaaaatg accccacttt attattaatt ggggcgggta tggcgccact aaagccatat
    16621 tttacggggg aaaaaacacc acctcattcc cgagtcgcca cctgtcaaaa gtgtgtgcgt
    16681 acgccggaca tcgaacgggt agggcgtaca gcacgccacg gcacgttttt tgagatgtta
    16741 ggtaattttt cttttggtga ttattttaaa gaagaagcga tcgagtgggc gtgggaactg
    16801 gtaacaacgg tgtataattt gccagaagat cgactctatg cgagcattta cctagatgac
    16861 aatgaagctt ttgatatttg gcataaaaag atcggcttgt cacctgaccg tatttttcgt
    16921 ctgggcaagg aagacaactt ttgggaaata ggtcaagggc cctgtggccc ttgttcagaa
    16981 atatattttg atttaggacc agaacatggt tgtggtcggc ctgattgtgc agtgggctgt
    17041 gactgcgatc gctttatgga agtctggaat ctagtattta cccagtttag ttgtactgca
    17101 gagggagaat atgtcccctt ggcacaacgc aatattgata ctggtgccgg gctggaacgc
    17161 ttggcggtag tcctgcaagg ggttaataat ctttttgaga ttgacaccgt gcgacccttg
    17221 ttagatcatt ttagcgaggc cactgctact aattatggac aagcagcaga cacggatatc
    17281 tcgctgcgga ttatcactga acatttacgt ggcatttcct ttatggtggg tgatggcatc
    17341 ctgcccggca acgaaggccg tggttatgtc ttgcgcaggt tattgcgccg tgcagtacgg
    17401 catggtaaat tgttaggtgt taacgaacca tttctctaca gagcagtaga tttagtcgtg
    17461 cgtgaatatg gtgattttta tcctgaacta tcgcaagggc aggagtatat taaacaagta
    17521 gtacagcaag aagaagatcg ttttcatgag acgctggaac aagggatgaa gattttaacc
    17581 gagctcacga atcagcttgg taccaagggc actttagctg gtgcggaggc ttttaaacta
    17641 tatgacacct atggtttccc catcgattta acgaaagaaa ttttagccga acagaacttg
    17701 cagctagatg aggctggctt taaagaggcg ctggaagcac agcgggagcg ggcacgcagt
    17761 gcgcgtgggc agcacggtta tggcactgaa gttacttcct atcaccactt acgcggtctg
    17821 gaaagcgaat ttgtgggcta tgagcagctg cacacagaaa gtaaagtgct ggtgattttc
    17881 aaaaacggtc aaccggtaac agcagctgcg gcaggtgatg aagtagaagt tgtgctctca
    17941 cagaccccat tttacgggga acgcggcggt caagtagggg atagcggctt gctttcctca
    18001 ccgcaggtga tgctagaagt taaagctgcc attatggcgc cggatggtca ggttgtgcac
    18061 cagggcacta taacttctgg tcagctgcaa atgggggata gtgtccaagc agtggtgcag
    18121 catgaaaaac gtggtgccat ttgccgcaat catacggcga cccacctctt gcatcaagca
    18181 ctgcgggaga tcttaggttc ccaggtgcag caagctggtt ccctggtgga caaggatcgt
    18241 ctacgttttg actttaatca tttaagttca ctgaccccgg agcagctagc atcggtggag
    18301 gcgcgtgtta acgagaagat ttgggagaac ctacccgttg aagttacttt aactagttta
    18361 gatgaagcca gggagatggg tgctactgcg ctctttgagg ataaatatgg tgatcaggtg
    18421 cgcgtcgtcc gggtagctga ctatagtatg gagctttgtg gcggcactca cgtcactaat
    18481 gccggtcaaa ttggcctctt taagattatt tccgaggcag gaattggtgc tggcttaagg
    18541 cggattgaag cagtcaccgg ccccggtgct tatcagtggt ttaaccagcg cagccagatt
    18601 ttagagcaag ctgctgcgca gctaaaaagc accgctacgc aagtaccgga gaaaattagt
    18661 ggtctgcata ttgaactcaa agaaatacaa cgtgaaaacc aacgactgca gctgaaactg
    18721 gcggggatgg aagtagatgg tttattagcc aatgtgtctc gagctagcgg cgtgccggta
    18781 cttagtgccc gtgtcagtgc tggcaatatg gagacattaa gggaaatggc tgatcgcctg
    18841 aaaagtaaac tggactctgg gattattgtg ctcggagctg ctgctgaagg taaagtatta
    18901 ttagttggtg cggtaacagc agatttagtt aaacaaggct atcacgccgg gaaactgatt
    18961 ggtgctgtgg ccaaactaac aggcggcggc ggcggtgggc gtccagacat ggcgcaggct
    19021 ggtggtaagg aacctgccca gctagatctg gctttggcaa aggtgaatga atttgtacaa
    19081 aaacagcaaa aataaaggaa atattaggat aaggtagaat aactctactg aataatgatc
    19141 ctatccttgg gggtgaaggt atggaatctt ttgatcagac catgaagttc aagcgaactc
    19201 ctgatgatga aacgtaccag gccagggatg tctttgcggt ggtatacagt gcattgaagg
    19261 aaaaaggcta caatcctatt aaccaaattg taggctacct cttgtccgga gaccctgctt
    19321 acattaccag ccatcaaaat gccagagttc ttattcgtaa gttagaacga gatgagttat
    19381 tagaagaatt ggtaggatca tatttagcaa gacttgatca cgaggcgtaa ctatggaata
    19441 tcgtaccttg ggaaagacgg ctatccgtgt ttcccgtctt tgttttggta ctcttacact
    19501 gggaccgctg cagtctaacc tatctcttca ggagggggtc cggctgttgg aaatagcttt
    19561 agatttgggc attacttttt tcgatacagc cgacctttat ggaacttacc cttatctgcg
    19621 cgaagcgatt cgccaccgcc cgcagcagcc ggtgatttct tctagatcat acgactatac
    19681 cagagaaggt atgcaggcta ctttgcagcg ggccttaaca gaattggctg ttccctatat
    19741 tgacattttt atgctgcatg aacaggaatc tccgctgacg ctggcaggtc accgtccagc
    19801 cttagagtat ttgttcgaag ctaaagcacg gggtttagtg cgtgccgtcg gtttttccac
    19861 gcatagaatc gccgccgtac aggcagccct caccatgccc gagattgatg tggtgcatcc
    19921 attaattaat cgccttggcg tgggtattca agatggtact gctgaagaaa tgctgtcagt
    19981 acttacgcaa ttgcaccagc agggtgtcgg tatctttgcc atgaaagcgt taggcggtgg
    20041 tcatttaata ccacagagtg aggcagcatt gcagtatgtt ttatcacaaa cttgtctaga
    20101 tgccattgct gtcgggatgg tgaacgagga agaaatacgc tgcaactgcg ctttgtttgc
    20161 cggggaaact gtttcttccc aagtaatgga gcatctgcag cgcaaaaaac gccagctcgt
    20221 ggtccaagag tggtgtgttg gctgcggcac ttgtgtaaag cactgcccgc agggcgcact
    20281 gcatgtggaa aatgaacgtg tcgtggtagc tgcagaaact tgtcttcttt gtggctactg
    20341 tggggcacat tgccctgact tttgcctaaa aatatattag taatgaggta aatatgcgca
    20401 tactgggact tgatgtaggt gatcgtacca tcggtgtggc cgtcagcgat gctttaggct
    20461 ggaccgctca gggcatagaa gttattcgtc gcgggacttg ggaagctgac gtccagcgtt
    20521 tgagagaaat ctgtgtggag tacgatgtca ctctgatagt agttggttat ccgaaaaata
    20581 tgaatggcac cattggtccc cgggctgagt tgtctgcaca atttgccgaa caaatttcgc
    20641 aggcactgtc actgcccgtt aaattgtggg atgaacggtt aactacgatg gaagcagaac
    20701 ggatgctgat tgctgccgat gtcagtcgcg ctaaacggcg taaagtgatt gataaaatgg
    20761 cagctgtgct gattctgcag aattatcttg cggctcaaac cgttaccaaa acggataaat
    20821 aacggtaaat ttgtaaagaa tatgggcgtt tactcaatag aaggaagagg tgtataatta
    20881 aatgacagat aaaatgcaag aactacaaga cgaagttgta accctgatcg atgaagaagg
    20941 tcaagagcat caatttatgg tcttagatat catcatggtc gatgacagtg aatatgccat
    21001 tatgattcct gctctagagg aagaggatac ggaagaggaa gaacaagaag caattatttt
    21061 ccgtattgat gaaaatgatg gtcaacagtc tttaactgtg gttgacgatg atgatgagtg
    21121 ggacgctgtg gctgcagctt gggaagagct agctgccatg gaagacgatg agtttgacga
    21181 cgaagaataa gctagagaga taacacggag gtagcgcgta tgtcaaaggg gaaagcgcgg
    21241 cggctgctag cggggatctt gattgtgcta gtcgtagcag tagggttatt tgccatacaa
    21301 ttaaatactt ggcttaaacc agtgccggcg atggctcagg aggctgtcat ggttgccatc
    21361 ccttcaggat cttcttccat gcgggtcgct aatattttag cggaaaacaa actaattcgt
    21421 aatgctgctg tttttcgcta ttatgcaaaa tttcgcggta tggatcaagg tttacaagcc
    21481 ggtaactata tatttagttt tggtatgacg atggatgaaa tcctgcggca cttgttggaa
    21541 ggcaatgtgt acagaccgac agtcacagtg actattcccg agggctacac ggtagaacag
    21601 attgcccaaa ggcttgaagc gacgggacta gtagatgcgg aagtcttcct cacattggtg
    21661 gcgtcaactg aacccgaatt aggcaaagtt gtcacagggc agcgttatgc aatggaaggt
    21721 taccttttcc ctgataccta tgaattcgat atcaatgcta ccccacaaca aatcttgcag
    21781 cgtctgcagg cacgtctcgg agaagtcctc acgccagcta ggcgtgaacg ggctgcagct
    21841 ttagatttgg acattcacga agtactgacg ctggcctcgt tggtggagcg ggaagtacaa
    21901 gcggctgccg aacgtgatat ggtcgctggc gtaatgttca atcgtttgca gaaaaagatg
    21961 cctctacaac tttgtgcttc agtaatttat gctttaggtg tgcacaaaac agaaatcagc
    22021 acaaaagata cccaagtaga gtcaccttat aacacctata aatattccgg cttgccgcct
    22081 ggaccaatag catctcccgg cgagtctgcg attgatgcag tgttgaatcc ggcagatgtg
    22141 gactatcttt actatgtggt taaaggtgac ggcagtggtg aacattattt tggtctcact
    22201 ttcgctgaac accaagctaa tattgcaaaa tcacaacaaa atgaaaaaaa acgtaagtag
    22261 gcgttaccat gcaaaaaata gaattacttg ctcccgcagg agatttagaa aaacttaaaa
    22321 tggccgtact ctatggtgct gatgctgtct atctagccgg caaaagtttt ggcatgcgcg
    22381 cttttgctgg taatttcggt gaagaggaga tgcgcgaagg catcactttt gcccatcagc
    22441 atggtgccaa ggtttacgtc actgttaata tttttgccaa caaccaagat attgcagagc
    22501 tgccgccata tttagagctg ctggctgctt taaaggtaga cgccctgata attgctgatc
    22561 cgggcgtact tgctgtggtg cggcaaactg taccctcact gccctgtcat ttaagtacac
    22621 aggctaatac aaccaactgg cgcagtgcta aattctggga ggacgcaggc ttttcccgtg
    22681 tagtgctggc gcgcgaatta accttggcag agattactga aattagccag caaactaacc
    22741 tggaattaga agtctttgtg cagggtgcca tgtgttgggc ctattccgga cgctgctatt
    22801 taagtcagca cctggcaggt cgtagtgcca atcgcggtga gtgtgcccaa gcctgccgct
    22861 ggaaatataa tttagtccag gaacggcagc caggggaata tatgcccata gaagaagatg
    22921 aacggggcat ctatatttta aatgctcgcg acctttgttt actcgaccaa ctgggcgagc
    22981 tagcccaggc tggggttaat agctttaaaa ttgaaggacg catgaagagc gtttattatg
    23041 tagcgactgt cacccgtgtt tatcgtcagg ccttggatgc ctatttagca gacccggaaa
    23101 actatactat cgacccagcg tggcacgagg aactgcataa aatcagccat cgcccctaca
    23161 ccaccggttt tatcgacggc aatcctgggg aaagcggcca agttttcgat agttccgcac
    23221 aggtttccag ccatgaattt gtgggtgtag tgcgggctta tgatgaagct gccgggcgcg
    23281 cagtagtgga aatgcgcaat cgtttcgctg caggtgaaag cctggaggtc gtgggtcccc
    23341 atacttccag ccgcgagttc actgtcagcg ccctaaaaaa tagtgaagac caaccaattg
    23401 ccgaagcaat tcgcgtccaa gagcacgtct ccttctctat cccctttccc gtagaagaat
    23461 attccctggt ccgacgccaa aaaacgtgac agctctgcga aagggtgcga caggggacgg
    23521 ttctcgctgt cgcatttgaa aaagcgctat cccccctgag taaaagggga tctttacctc
    23581 ttagaagatc ctttggttta acattgtaat gcgacaaaga accgtcccct gtcgcaggtg
    23641 taaatacata aaaatggccc ctgttagaca gactaaagtg ttgagtctgt agatgggggt
    23701 ttttttatgc gcagggagtc aagacaaaaa agaattgtgg taatgatagg ctgcttacta
    23761 ttggctgtaa gtgcactagt cggtcgtctt ttttacatac aaataattaa agggcgtgac
    23821 tacgcagaaa gggctgtggc acagcgatct gcagcttatg tttatgattc gggccgcggc
    23881 caaatcctgg atcgtaatta tcaatcctta catgccatca gaacaaagga ttcgagtaga
    23941 ctacaagccg aattagttgc ttacaacatc ccgcgggact tactaacagg cttggaaatt
    24001 gaagaagaaa tccgctacca tgcgggctct ttggcttcgc atgtcacagg ctatattaaa
    24061 agaccacgta atccgatgca ggcgcaaaat ggtttagctg ggttagaacg cgcttttaac
    24121 aatgagctgt ggggctctcc tgcggccatc ggtgttatta tcgatgccaa ccgagagatg
    24181 gttgccggtt taggaattaa tgagtggcag tatgaacatt tccgacgtcc ttatcatgta
    24241 gttaccacca tcgatcggca attacaaggg attgtggaaa cagttggtgg cagtctgatc
    24301 aagaaaggtg cagtaattct gctagagccc aaaactggtg acattgttac catggcttcc
    24361 tttccccgac tgttggtaga aaaattatat agtggtgtag aacaacaaga actggcggac
    24421 atggaaaatg ggaatgccta tcttaatcgt agcttgatgc agtaccccac cggttcggta
    24481 tttaaagtta tgttagcggc ggcggcgata gaggaacagc tgccggagcc agaaggactg
    24541 tttctttgtg atggcagcta tgaggtaggg aaccgcacta ttagctgcta taacggtacc
    24601 agccatgggg tagtcgatct ccatgcagcg ctggcggttt cttgtaacgg ctattttgtt
    24661 aatctggctg aacgccttgg gtcagaaaaa ttgttagcaa tggttagacg ctttaagttg
    24721 ggacaagcga cgggtgtccc cttgggcggt gaaatgcctg gtaaaattcc taccgccgct
    24781 gaattacctt accccggtga tttagcaaat acggcaattg gtcaaggttt agttgccgct
    24841 actccgctgc agttggcacg aatgatggca atcattgtta acgatggacg ggatatttat
    24901 ccgcggctgg tttcacagat aattgataag aacggtaatc ctgtgaaaaa ttatcctgtg
    24961 caatatggta caagagtagt atcaccgctt atcacgcgcc aattaaaaaa aatgttaact
    25021 actgttgtta acaatggttc agcgaatagc gccgcaagtg aactatatca ggccgcaggt
    25081 aaatcgggga cagccgaaac gagtaagacc ggtgtcagcc attcctggtt tgccggttat
    25141 gttgatctga acggtcgtat tttggttgcg gtagcttttc ttgaagagtg gcggcctaaa
    25201 gagccgacag cctccagtgt tttaaaacaa ataatggaag ctatcgctat tgcccaaagt
    25261 tagttatcag aagtacctgc gacgtgtcct ttccagaaag ggggcacgtc gcttttgtct
    25321 gcagaagtaa tacttgactt ttattagtac tgcggcttag acttttagta tgtgccaaca
    25381 aaaagtagga ggtagaagat ggtttcagag aaagatatgc acgaaggtaa tatatttaaa
    25441 ctgattttaa acttttccgg cccagcgatt acaggaatgt tggtaatgtc ggtttacaat
    25501 attgtcgatc gtatctacat tggccgtggc gtaggtactt taggtattgc cggtatagcc
    25561 attgggcatc ctctaatgat gattaaaggt gctatggcca tgctagtggc cataggttcc
    25621 tcggcgatga tttcgattaa tctcggccaa aagaaaaaag aagcagccga aaaaattctt
    25681 ggtcatggct ttactttgct agttgtcttt gctatttttg cttctggtgt tagtttagta
    25741 tttttaactc cactaatgcg gctctttggt gccagtgaaa atgtgctgcc ttatgctacg
    25801 gattatatga gagtcattct cattggcaat gtctttcaaa tgattggtta tggtgctaac
    25861 aacttcatcc gggcagaagg gaacccacgt actgctatgt tttcctccat aattggtgcg
    25921 ggaacgaaca ttattcttga tcctattctt atttttgttt ttgaaatggg agtaacaggt
    25981 gccgccgtgg ccacgattat ttcccaggcg gcctcagctg cttgggtact gcactactat
    26041 cttagcggta aaagttacct aaaaattcgt aaaatcaatt tgcgtccaga attaccgctg
    26101 tctttgaaga tgatgtcctt gggcacctcc tcctttgtac gccaggtgtc taacagtatg
    26161 tctattgtct tattaaataa tagtctgcag cactatggtg gcgatatggc tgtttctgcg
    26221 atgggtgtta tttttagtat tcgtacctta cagatgatgc ccgttttggg tattagccaa
    26281 ggtgtgcagc cgattatcgg ttacaattat ggcgcacaac agttaggacg ggtaaagaaa
    26341 gcttttaagt tagctgtctt actggcgact gctgctaccg tacttaattt tgctgctatt
    26401 atgctcttcc ccgagttctt tttccgtctc tttactaccg acgctgaact tattgcggta
    26461 ggagcggccg gagtgcgtcg ctatatgtta tttttaccta ttttaggcta tcaagtttta
    26521 ggagccaact atttccaagc agttggtaag ccaaaacaag caatctttct caaccttagc
    26581 cgtcaggcca ttttcctgat tccggcctta ctgttcctgc ctcgttattt tggtttgagc
    26641 ggcacctggt cagcccaacc aacagctgat gctttatctg ttttggttac gacagcatta
    26701 gtctggtatg aaatgcgccg ccttacccag caggataagc aactgcaaca attaacaaat
    26761 tagaagagga catcaacaag gaggatgtag attgtgattg attcaactat tctatatctt
    26821 atggcagcac ctatttattt agctgtttta atagtggtca ttaaaaagca agttcccata
    26881 caaaaacagg tgctaattgc tttgtttttt atttatctga tcggattttt ttcagtgaca
    26941 ctttttcctt taccggttca aaatatagtg tcccccttgt caagacaaag gttttcatcc
    27001 ttttaatgaa ccagatattt ccttttcctt taattcaggt ggtacccatt cagcgggaat
    27061 caagggcgag cagagcgagt tcatcttgac ccttgagtcc ctcactaaac cagaatcctg
    27121 ttctgcggtg gtctaaagct agaccacctg ccctccggcg aggacggtgt ttggggctgg
    27181 acccaacgtc cctaagctgc tattcttccc tgaaatactt cgcatggagt cagatagcca
    27241 tgtgtttggt ggggtcgctc agtgttgtat tcttgaacgt attcccggat tccaatccgt
    27301 aatacttttg gtgtaagaaa ttcaaagggt agatacgctc agtttttagg ctccgtaacc
    27361 atcgttcaat gatgacgtta tccacccaac gccctttgcc atccatactt tgtataatag
    27421 cagaaaagca aattggaaga cgactgtttg atggattgat ttaacatgtt tgtattgcct
    27481 tgattgtgtt tctcattctc gcttgggggg ggggaatgga atttagtcgg gatacagaag
    27541 gattggcata ttatctgtcg aaaagaatac agcggaggtg tagtatggat aacgaaaacc
    27601 aaatctttga gcttatgacc aaaatgtatg ctgaaatgca gaaggg
//
LOCUS       DASRMS010000103         2887 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_295691, whole genome shotgun sequence.
ACCESSION   DASRMS010000103 DASRMS010000000
VERSION     DASRMS010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2887)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2887)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2887
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295691"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VFC74_04780"
     CDS             <1..265
                     /locus_tag="VFC74_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24685.1"
                     /translation="APAFGRQGPPHCATKSYPLQAIISTINGQPSAALKRKEGAGFAP
                     ASRLSLLAFTGHKCGRLPAALKRKVGAGFAPAYRLYPRSGKNE"
     gene            896..1144
                     /locus_tag="VFC74_04785"
     CDS             896..1144
                     /locus_tag="VFC74_04785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24686.1"
                     /translation="MEEKELACDKMRRMVEWLNYQDENFNDKFQSVADIEKCFDACER
                     HGLEFADNLDWEDDDTGYEEYKLACEVNEILGYKYYRI"
     gene            1231..1488
                     /locus_tag="VFC74_04790"
     CDS             1231..1488
                     /locus_tag="VFC74_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24687.1"
                     /translation="MEETRKETLMRIGMRLVDKLLADASPHLLSDKENVEFEFEKASF
                     TLAIEDLGTLLDSYLDDDIDISYDAAHNLNALYTICKLSKL"
     gene            1533..1736
                     /locus_tag="VFC74_04795"
     CDS             1533..1736
                     /locus_tag="VFC74_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24688.1"
                     /translation="MDRLLVDGKAYVMPEQACVVFKMPTMCVTITRDELDAAIMDYCE
                     GHLEITKKAAYRATIYIKQLKRL"
BASE COUNT          865 a          571 c          753 g          698 t
ORIGIN      
        1 cgcgcccgcc tttgggaggc aggggccacc ccactgtgcc acaaaaagtt accctctgca
       61 ggccattatc agcaccatta acgggcagcc ttcggcagcc ctaaaaagga aggaaggcgc
      121 gggcttcgcc cccgcctctc gtctgtcttt gttggccttt acaggccaca aatgcgggcg
      181 gcttccagcc gcccttaaaa gaaaggtagg cgcgggcttc gcccccgcct atcgtttata
      241 ccctcgcagt ggcaaaaatg aataattatc caaaactgaa aaaccaaaat gaataattat
      301 ccaagcacag cccttggcac agaccgctag agaggagcgc gcgcgcgacg gggggcgggc
      361 gggggcaagg gcgaacggtt gggatgctgc tgcctgatgg tcgctgataa tggctggata
      421 tggggcgcgc gaggcgagcg aagcgagccg agcatctgcc tgctgcctgc tgcctcctgg
      481 tggcttatgg tggttatggt ggtcaaaaaa aaccgaactt tgtgttatat aacatattat
      541 tgactattat tatgttaatt attgttattg atattatatt actaagacaa atatttgtat
      601 atgtcttaga tatatattat ctttgcagtg attttttaac ctataatata aaacgatatg
      661 actagagatg attttgatta ttaccgtaag gccatcatga gagggtccta ttatcggatt
      721 gtgccaatgc gtggtaggtg ctattttgag attctggatt ataccgacca atggttaggg
      781 gctgaatgta gcaatgagga tgttctgtct ttgttatcag ggacaacatg caagagtgtg
      841 cctggagagg tttgagagtt tgaaatttta ttcatgaata tttatgtagt ggaatatgga
      901 agaaaaagaa ctagcgtgtg ataaaatgcg ccgtatggtt gaatggctga attatcaaga
      961 cgagaacttc aatgataagt ttcagtctgt tgctgacatc gaaaaatgtt ttgatgcttg
     1021 cgagcgtcac ggtttggagt ttgcagataa tctagactgg gaagacgatg atacaggtta
     1081 tgaagaatat aagttggctt gtgaggtcaa tgaaatctta ggttataaat attatcgcat
     1141 ataatttacc aagtacaagg acattctgaa aggagaagat tagttttaac ctgcctgccc
     1201 gctctgcggg cgggcacaat agaaagaagt atggaagaga ctagaaaaga aacattgatg
     1261 agaattggca tgaggttagt ggataagttg ctagcggatg caagccccca tctactgtca
     1321 gacaaggaga atgtcgaatt tgagtttgag aaggctagtt ttacacttgc gattgaggat
     1381 ttgggcactc tgctggacag ttatttagat gatgatattg acatctctta tgatgcagcg
     1441 cacaatctaa atgctctata tacaatttgt aaactttcta agctatgaga cggaaatcaa
     1501 gagaattgtt gcagaatcta ggagggcggt ctttggaccg cctcctagta gacggaaagg
     1561 cctatgtaat gcctgagcag gcttgcgtgg ttttcaaaat gcccacaatg tgtgtgacca
     1621 taacaaggga cgaattggat gcagctatca tggactactg cgaaggtcac cttgaaatta
     1681 cgaagaaagc agcctatcgc gctacgattt acatcaaaca gttaaaaaga ctatgacatt
     1741 atattgtttt gctatgataa tggcagcggc tggcgcagtg tcaggcgcag ccgccaccct
     1801 attgtttaca aagtataggg atattctgaa agaagaggaa gaagactagt tttaacctgc
     1861 ctgcccgcta tgcgggcggg cacaacaact gaagtatatg gagaaaagac atgagttttt
     1921 gaatgcgctg aaatacataa acagcggcta cgaattccgc ttttaccgag agaaggtgga
     1981 gagtgatttg tgtatccttg aaatctacgc agaggacacc aaacgcataa agttcgagtt
     2041 gcgcgagtgt gagattacaa tactgctggc ctacaagaag ctagttattg cggatatgtt
     2101 ggcttgataa ttggaggtga tattatgatg tacagattag ttattaaatt gaaaggagtt
     2161 acctattact ttgagtgtga cacgataggg gaggcctaca gggtagccaa attggtagcg
     2221 gctatcctaa aggtgcggac aaggaagccg acaagaaaaa ggtggataat atggactaca
     2281 ctcgaatgct tttatgagta cgatatattg cacgaaaaag acaaaagttt tgacatttct
     2341 atgatgatac gctccaaatt cctatcttat gactattggg ggaagccaag aagctatgta
     2401 gaaaaacatg cctgaatgag cgaaaacggg ggaatgattt ggaatgatca ttcccccttt
     2461 tcttatgcac gcgctccatc aggatgggcc actcaggaga agaaaccaaa agcaccaaaa
     2521 agacagaggt cccgacacag aggctaatga gtagaaaaga tgttgacact gaaattcagc
     2581 tggccaacac ccatcagaag cctgacggcc ccaagtagcc atcaggagca gccagcagcc
     2641 atcaggagca cttggccagc tggacaacca gggagggaga gcctgaggca taggcagcag
     2701 ccgacaccaa gaagcaccca ccaccaggca gcgcgcagga ccaggcagca gacagcatta
     2761 gacaaaaatt gtccaagatg tccaacaccc atcagaagcc tgacggcccc aagtagccat
     2821 caggagcagc cagcagccat caggagcact tggccagctg gacaaccagg gagggagagc
     2881 ctgaggc
//
LOCUS       DASRMS010000104         1394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_765237, whole genome shotgun sequence.
ACCESSION   DASRMS010000104 DASRMS010000000
VERSION     DASRMS010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_765237"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="VFC74_04800"
     CDS             <1..248
                     /locus_tag="VFC74_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GDP-mannose 4,6 dehydratase"
                     /protein_id="HZK24689.1"
                     /translation="INIASGRAVPIEQLLKILLSHTDASIEVDLDPQRLRPSDIPSAS
                     GDARILLEQTGWHPSFTLDETLLAVLNDWRQRVRGLA"
     gene            245..>1394
                     /locus_tag="VFC74_04805"
     CDS             245..>1394
                     /locus_tag="VFC74_04805"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZK24690.1"
                     /translation="MKVLLNGQALQAPLTGIGNYTLNLLQSLQSLPSISAVDCYFNNQ
                     FVSAQEVLERLQLLNSVVTPVQPNLKRSVKQCVRALPLAYQARDWLQSHHFRQQSMRH
                     NYHVYHEPNFILKSYRGPAVTTVHDLSFIYYPEFHPAERVRWLQRELPKTLARAERVV
                     TDSERVKQELLERFNLPEDKVHAIHLGVAEEFRPHNLASCIDALNSWQLRYKSYVLFV
                     GTLEPRKGIAVLLAAWLKLPKALQREFPLVIAGAPGWKNTALMKQLKELHQQGKIHLL
                     PYVHETVLPYLYAGASVFAYPSVYEGFGLPVLEALASGVPVICGQDTAMAEFAQGACL
                     VEDLDSVDQWYEHLQQQLVNNYPLALRDCQHAQEFTWQRCAERTAQVYA"
BASE COUNT          335 a          293 c          367 g          399 t
ORIGIN      
        1 ttattaatat agcttcaggg cgagcagtgc ctattgagca gcttttgaaa atactgctta
       61 gccacaccga tgcttctata gaagtcgact tagaccctca gcgattgcgt ccgtctgata
      121 tacccagcgc atcgggtgat gcgcgtattt tgctcgagca aacaggttgg catccaagct
      181 ttacgttgga tgaaaccttg ttggcagttt taaatgattg gcgtcagcgg gtgagggggt
      241 tggcatgaag gtgttgttaa atgggcaggc cttgcaagcg ccgctgacgg gaattggtaa
      301 ttacacctta aatttattgc agtcactgca gtcgttaccc agtatttcag cggttgattg
      361 ttacttcaat aatcagtttg taagtgcgca ggaggtttta gagcgcttac agctgcttaa
      421 ttcagtggtt actccagtgc agcctaacct gaagcgcagt gttaaacagt gtgtcagggc
      481 cttacctttg gcttatcaag cgcgagactg gttgcagagc catcattttc gacagcagtc
      541 tatgcggcat aactaccatg tttatcatga gcctaatttt attttaaaaa gctatagagg
      601 ccctgcagtg actacggtgc atgacctatc atttatttat tatccagaat ttcatccagc
      661 agagcgagta cgttggcttc agcgagagct accaaaaact ttggcgcggg cagaaagagt
      721 tgtaacggac tcagagcgag ttaagcagga gctattagag cggtttaatc ttccggaaga
      781 caaagtgcat gcgattcact tgggggttgc tgaggagttt cggccgcaca acctcgcttc
      841 ttgtatcgat gctttaaaca gttggcagct gcgctataaa agctatgttt tgtttgtcgg
      901 cactcttgaa ccacgcaaag gcatagctgt tttgttggca gcttggctga aattgccgaa
      961 ggcgttacaa agagagtttc cgctagttat tgctggggcg ccaggctgga aaaataccgc
     1021 tctcatgaaa cagttaaaag agctgcacca gcagggaaaa atacatttgc tgccttatgt
     1081 gcacgaaact gtgctgccgt atttatacgc cggtgccagt gtctttgcct acccgtccgt
     1141 ttatgagggt tttggtttgc cagttcttga agctttggct tctggtgttc cagttatttg
     1201 tgggcaggat actgccatgg ctgagtttgc ccaaggagct tgcctggttg aagacttgga
     1261 ttcggttgat caatggtatg aacatttgca gcaacagctg gttaataact atccgttagc
     1321 tttacgtgac tgccagcatg cgcaagagtt tacttggcag cgctgtgcag agcgtacagc
     1381 gcaagtttac gcag
//
LOCUS       DASRMS010000105        46893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_698782, whole genome shotgun sequence.
ACCESSION   DASRMS010000105 DASRMS010000000
VERSION     DASRMS010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 46893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46893
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698782"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1367)
                     /locus_tag="VFC74_04810"
     CDS             complement(30..1367)
                     /locus_tag="VFC74_04810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005283 - amino acid:sodium symporter
                     activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HZK24691.1"
                     /translation="MSIQSLNFFLQEFVGGPIMIAAVLLTGCFLTLRLNWLQVRHFST
                     MLRDTIFGLRRQKNTGIGEISAFQALTTALAGTIGTGNIAGVATAITLGGPGAVFWMW
                     VSAFVGMVTKYAEVVLAVHFRDTQPTGRVVGGPMYFLAKGLNSRLLAALFALFGSLAA
                     FGIGNMVQANSVAAALHEAFNVPQLITGIVMAAITALVIIGGISRVAAFTEKLVPLMA
                     AFYTAGALFIIFLNLAQVPLAFSQIIGNAFTGRAAVGGFAGATVLQTFRFGVARGIFT
                     NEAGLGSASIAHAAARTDHPARQGLWGMFEVFFDTHISCTLTALAVLVTGVWNTGLDG
                     AAMTTAAFNQALPSWGGYFVAVSLVIFAFSTLISWSYYGEKCFEYFVSTRATLFYRLL
                     WIALIPIGAVGGLHVVWALADTLNGLMAIPNLIGLWGLNGLIVKLTREFFQKR"
     gene            complement(1437..2804)
                     /gene="amrA"
                     /locus_tag="VFC74_04815"
     CDS             complement(1437..2804)
                     /gene="amrA"
                     /locus_tag="VFC74_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HZK24692.1"
                     /translation="MRTVVHAGIYPHPAIVIPAVGGSEAKLVAETAAAMSELAGRIKQ
                     SGADVLVLITPHGPMFRDAVALLADEQLTGSLAQFAAPEITFSWPNEKSVLQAIELEA
                     ERFGINCAHLDNRRSAAYGVEVTLDHGAMVPLYFLQQRGIRLPLVHITYGLLAAKQLY
                     SFGQAVRKALLRLKRHAAVICSADLSHRLKEDAPAGYSPAGQEFDQKLIHLVERFDVE
                     AILNLNHRLVEEAGECGYRSLIIALGILDGETVEPEILSYEGPFGVGYLVADLTPGRK
                     RTLQPFASTRTQESEHVQLARHTLETFVRTKQVITPPTASSLCQQRAGAFVSLHLNGE
                     LRGCIGSIEAQQANLAAEIVENAISAGIYDPRFPPVTAKELSGLVYSVDVLAAAEAVD
                     SMAELDPQQFGVIVEEGQRRGLLLPNLSGVETAEVQVSIALQKAGINPSELYQLYRFR
                     VQRYY"
     gene            complement(2957..3697)
                     /locus_tag="VFC74_04820"
     CDS             complement(2957..3697)
                     /locus_tag="VFC74_04820"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HZK24693.1"
                     /translation="MRIFFIGSQRTRRKYLLICVYLLCLLILVSVIKIVLPVSKAITV
                     QGCLPLANKTIVLDPGHGGYDPGVRNNNVEEKNIVLAIALQLRDYLQASGARVVMTRE
                     TDTDLLVQPTAGPKKQEDMRNRMQIVEAADPDIFLSIHANAIGSAQWRGAQVFHKIEH
                     QQSIILAQALQQELVRVLGNTDRVIKPGNYFVLNETERCAALVECGFISNPEEAQLLT
                     DPAYQNKVAWALYLGLIHYYAQPPAVVD"
     gene            3759..4325
                     /locus_tag="VFC74_04825"
     CDS             3759..4325
                     /locus_tag="VFC74_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZK24694.1"
                     /translation="MKNSSLLQLFLTFFKIGATTFGGGFAMLPIIKREVVERYAWLSD
                     EEFVDTLAVTQSLPGAIAVNSAIFIGYRVRGYAGALLSMFGAVLPSFLVILFIATFFA
                     RFAEYPMVQAAFAGIRPAIAALIAAAVLKVGKPVLKNCQSIIFAAAFLLLSEGLGLHA
                     IIVILLGALSGLLMHYLKTKKNCEVDAQ"
     gene            4322..4843
                     /locus_tag="VFC74_04830"
     CDS             4322..4843
                     /locus_tag="VFC74_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012062323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HZK24695.1"
                     /translation="MILLQLYLAFLKMGFFSIGGGYVMLSLIETEIITIRGWLSLPEF
                     VDILAISEMTPGPIAINSATFIGYRIAGFWGSAVATAGVITPSIILVLLAATFLKRFY
                     QNQWVQAAFSGLRPAVIGLIAGAAIFVTRTALVDLPSVGIALGAFILLVFTRLHPILV
                     LALSALAGILINL"
     gene            complement(4895..5398)
                     /locus_tag="VFC74_04835"
     CDS             complement(4895..5398)
                     /locus_tag="VFC74_04835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZK24696.1"
                     /translation="MRKLTKHHVPGDKNSLYYWTSYVKPWRVVFNFIVIYLCRYLPSL
                     RLKNVLYRLVGIKIGKDVSVGLMVMFDVFFPQLITIGDNSVIGYNATFLAHEFLVDEW
                     ATGEVKIGANVLIGANSTVLAGVTVGERAMVSAHSLVNKDVAPQTMAGGVPIKKIREL
                     MENPQRH"
     gene            5715..5999
                     /locus_tag="VFC74_04840"
     CDS             5715..5999
                     /locus_tag="VFC74_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain containing protein"
                     /protein_id="HZK24697.1"
                     /translation="MYEDKTLTCRECQEEFIFTVGEQEFYAEKGFENEPARCPSCRAA
                     RKQRMNGDRSERRMYKVTCSECGVETEVPFNPSLDRPVYCRDCFQKNKSF"
     gene            6224..6925
                     /locus_tag="VFC74_04845"
     CDS             6224..6925
                     /locus_tag="VFC74_04845"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LiaF domain-containing protein"
                     /protein_id="HZK24698.1"
                     /translation="MQFDKSKSLLGIVVITAGIILLLDNLNIIAGINLFAYWPVLLIV
                     WGLQSLWTGSKNPAAQGISAFVIILGFIFLANSLTWAEINVGRVFQFFFPVLIILIGG
                     SIFLGRSFAGKANLAIMGAVERGKTSSWQLESGSYVAFLGGIDLDLRHAIIPEGETVL
                     DLTAVMGGIDIRVPADIPVVADGFAVLGGVEFFGKGSGGIVGSTHTEQNIGEGNQVLI
                     IQARAVMGGIDIKRV"
     gene            complement(7018..7179)
                     /locus_tag="VFC74_04850"
     CDS             complement(7018..7179)
                     /locus_tag="VFC74_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24699.1"
                     /translation="MEGISSIVILGVVIVVAVFILKTVLKVALRIALLAILGILTFLY
                     FTGNLPLPF"
     gene            complement(7290..9098)
                     /locus_tag="VFC74_04855"
     CDS             complement(7290..9098)
                     /locus_tag="VFC74_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003371305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24700.1"
                     /translation="MPTEKPKNFKKTTGRLIKYLKPHWLTLLLVFIAAVLGTVFTTVS
                     PKILGQATTLIFEGMMDRVNNVSGGGIDFARIRQILLMLIGLYITAAAFRYFEHYIMA
                     SVSQKTVYDMRLEVSEKMSRLPFKYYDAQTHGEILSRVTNDMDLIARTLQQSLAQVIE
                     SVVTFFGVLFMMLTISGWLTLITLVSIPLSIIITTLVARQSQKFFAAQQRELGNMNGH
                     VEEMYGGHLIVKAFNHEHKSVAEFNDINERLYDVGWKAQFVSGIIMPLIGFVNNIAYV
                     LVAVVGSIFVTQGAITIGNVQAFFQYSRQFSQPITNAANIANIFQSTIAAAERVFELL
                     DEEEEVADGEQALAEFVPKGNVRFEHVKFGYAEDSILMKDMNIDVQSGQTIAIVGPTG
                     AGKTTLVNLLMRFYEVNEGRITIDGIELRDIKRSTLRSIFGMVLQDTWLFKGTIHDNI
                     AYGREGATEEEIVKAAKAAHADHFIRTLPEGYDTILNEEASNISQGQRQLLTIARAIL
                     ADPDILILDEATSSVDTRTEILIQKAMGQLMKGRTNFVIAHRLSTVRDADLILVMDNG
                     DVVEKGTNAELLEMDGFYADIYNSQFSDLAAIGQTG"
     gene            complement(9230..10957)
                     /locus_tag="VFC74_04860"
     CDS             complement(9230..10957)
                     /locus_tag="VFC74_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24701.1"
                     /translation="MLKILRELKPFKFQVAIALILVVVRALTDLFLPTLTADIVNKGI
                     VNGDTQIIARIGGLMLAIAAFGGICAVASNYFASKSAMGFARNLRTKVFAHVENFSLH
                     EFDKFGTASLITRTTNDVTQIQMMLVMAIRMMVMAPVMSIGGLILAVSKDAKLSLIFV
                     VALPLLFGFITFVGRKGSPLFRAIQTKLDTLNLVLRERLTGMRVIRAFSREDYEKKRF
                     ETANLDYTNTSIRVHRIMSLMMPGMMMVMNLTTIAIVWFGGIRISYGEMMTGDLMAFI
                     QYGTHIMFSMFSLSMIFVMIPRAAASANRINEVLETVPSISDPSVEKTAEEAHGRVEF
                     KDVTFSYPGAERPALRNINFTAGPGEITAIIGGTGSGKSTMSSILLRFYELDSGSILI
                     NDVDIREMSQVQLREKIGYVPQQAVLFSGSVAENIRFGQEEATDAEIEHAVEIAQASD
                     FVKSMPDGFDSMIAQGGANVSGGQKQRLSIARALARKPEIYIFDDSFSALDFKTDARL
                     RAALKSETTEASVLIVAQRVSTVMDADRIIVLDDGEVVGMGTHRELMASNPVYQEIVS
                     SQLTEEEIA"
     gene            complement(10965..11435)
                     /locus_tag="VFC74_04865"
     CDS             complement(10965..11435)
                     /locus_tag="VFC74_04865"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK24702.1"
                     /translation="MDNADRETTQKLMDLFSRFRRLHRGCPIPGLKNSELRILFGVKK
                     SLTMQGCGARISDLSHVMRVTSPTVTQLVNSLESSGLVERTTDPQDRRSIRVVLTTKG
                     EETIQAAADEFFSEFYGLVKHLGEEKSQQLIELLVECFDYLRNSTPTKFAGETE"
     gene            complement(11614..13188)
                     /locus_tag="VFC74_04870"
     CDS             complement(11614..13188)
                     /locus_tag="VFC74_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK24703.1"
                     /translation="MKELKYSLTAKISAFCLLYLNGYILLISAYNFVVGGRIEELVVR
                     NIWPFSWFLTSAGIMLPLTIVVSILLFSFLLAAAGTQRNSEELVLNRWDRIPTDLISL
                     GCGTLFLLGVIILPITGGLGIGTITSYYDLSTDVGLGIYLSLILFYLYSIFMLWCITI
                     VKRLRTQTLLTNTLFYRLYDLLRKIFLHLTEVYQAGVAVIIFGLINITVIFNMHSGLA
                     MLFFLMFNSAVLLFILYLALQVSQLKQNMQKIAAGQLKVNLDSTKFTLTFKEQAQALN
                     AISAGLQKAVEERMSSERLKTELITNVSHDIKTPLTSIINYVDLLKKTALENEQALAY
                     LDVLEQKSYRLKDLIEDLMEASKASTGNLTVDLTEINVNELLKQITGEYDERLRERGL
                     EVIQTVPGEKVVINGDRRHLSRLLENLFSNVKKYAQPQTRVYLDLEATATEMKLIIKN
                     ISAEKLNISPEELMERFVRGDKARHTEGSGLGLSIARSLAEIQGGKFNIKIVGDLFEV
                     ELIFPVLKEEDLLQSG"
     gene            complement(13191..13886)
                     /locus_tag="VFC74_04875"
     CDS             complement(13191..13886)
                     /locus_tag="VFC74_04875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004614376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK24704.1"
                     /translation="MGQFNILVCDDDKEIVAAIEIYLANEGYQIKTAYNGREALAILN
                     SEQVDLVIMDIMMPEMDGIRATVKIREDKNIPIIMLSAKSEDTDKILGLNMGADDYLT
                     KPFNPLELIARVKAQLRRYASLGSREVKADVYASGGLVLDDQQKLVTVDDKPVNLTPL
                     EYNILKLLLINKGRVFSTDQIYEKVWQEAPVASDNLVSVHIRHLREKIEIDPKNPIYI
                     KVVWGLGYKIERL"
     gene            complement(13892..14767)
                     /locus_tag="VFC74_04880"
     CDS             complement(13892..14767)
                     /locus_tag="VFC74_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZK24705.1"
                     /translation="MQKYKMLKLSLAALLLLGVVELVFLEQEQVTNQTTLQRVKTEMI
                     AVTTVPVPQCDNDYHLLLVNEALPITVGESAFTLVPAYGHLPLKTADTMLEEKTLQQA
                     ELMIEAAQAAGMNNLVITSGYRSAAEQAQLYREAADKDYVAKPGTSEHELGLAIDLQI
                     LRNGFNSFLSSRQGAWLVEHAHQYGFIMRYPQEKAEITGIPYEPWHFRYVGIPHAAVM
                     AESKLCLEEYLAALNPGTVYQIKTETQNYLVYRVKTRENCIEIPEDLNYQLSCDGTGH
                     YIVTVEIGDIDEGAV"
     gene            complement(14923..16080)
                     /locus_tag="VFC74_04885"
     CDS             complement(14923..16080)
                     /locus_tag="VFC74_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HZK24706.1"
                     /translation="MQYRKFGRLDWQVSALGFGAMRLPTTDGNQANIVEYEAIDMIRH
                     AIDQGVNYVDTAWPYHHGQSEVVVGKALQDGYREKVRLATKSPIRFYKHAEDFERFLT
                     EQLSRLQTEQIDFYLLHGLNKNSWEQVVLKYDLLQKAEAAKKDGRIRYLGFSFHDDLA
                     AFKEIVDGHQWDFCQIQYNYMDTENQAGRAGLQYAAAQGLAVVIMEPLLGGKLVKPPQ
                     DITALLHAADPTRSPVDWALQWLWNQPEVSVVLSGMSTMEQVQQNLQAADKSGVNSFS
                     EAERQLMVEARQRFLTKTAIPCTGCAYCMPCPNNIEIPRNFKTYNEGFMYDDLTGARG
                     TYQHFLTEAMRAGSCLECRICEEKCPQGILISEWMPKVHGVLGEGKDYERN"
     gene            complement(16230..17021)
                     /locus_tag="VFC74_04890"
     CDS             complement(16230..17021)
                     /locus_tag="VFC74_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZK24707.1"
                     /translation="MKKVLAILMTLVMATVLLVGCAGGDKEPSGNGNGNGNQNPAPTG
                     DVKLYQGVGTASNFRNGPGSDSEGVPVYSYNFVFASAIFDAEGKVVDAEVDILEVSTP
                     NYDGAGMPHFSGWPGKEGYNITDEDTGKVTGVSENTEESVPKEIEEWVTKRERGDNYH
                     MNPDNDWHKQMDSFEKWFKGKTVAEIEDAFTTMFSDRNGRPLDPNTDNEEDKTKVDKL
                     TDEQKSEVEDIRSGATMSLSDAHGDILGALKDAYENRKEMAASTK"
     gene            complement(17233..17568)
                     /locus_tag="VFC74_04895"
     CDS             complement(17233..17568)
                     /locus_tag="VFC74_04895"
                     /inference="COORDINATES: protein motif:HMM:NF023184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HZK24708.1"
                     /translation="MALSSTLEEKTVTKMVDIYCQGNHGSTTELCADCNELLAYSKER
                     IDKCPFGEQKPVCGKCTVHCYAAQQREAIKKVMRYAGPRMLYKSPLLTVRYLYRKAFK
                     SAKNKKVKA"
     gene            complement(17672..18277)
                     /locus_tag="VFC74_04900"
     CDS             complement(17672..18277)
                     /locus_tag="VFC74_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK24709.1"
                     /translation="MPKTTFFNLNKEKQSKIFQAAVQEFSLCSFKEASINQIIKQAGI
                     SRGSFYQYFRDKKDIYLYMLTEIGKEKLAVIDQARQQQPDDDFFAALLHITEAALTWA
                     HAMPQYNQIGMRMILDESEFIIKLRELSPQAVAQLTSLIARDQQRGVIKSEVKPELII
                     EMITALMLYCLKEYYQSADYEGMLKQATEIYAIIKHGIAST"
     gene            18380..19231
                     /locus_tag="VFC74_04905"
     CDS             18380..19231
                     /locus_tag="VFC74_04905"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24710.1"
                     /translation="MITVRNLRYKYPRNKDYTINGIDFDINTGEIFGFLGPSGAGKST
                     TQKILIKLLRGFEGEINYLDKPLASFGDDFYEDVGVSFEMPISFSKLTALENLQFFQQ
                     LYKTKVEVEPLLRRVGLWEDRHKKAGEYSKGMKIRLNFVRALLNKPTFLCLDEPTNGL
                     DPANARIMKDMILEFKQEGGTVFLTSHIMSDVDELCDRIAFIADGELREIDTPRNLKI
                     KYGKRSVNVEYRDQGELIKREFSMAEIKSPAFINLLQTKEIETLHSGETTLEEIFIKV
                     TGVALRD"
     gene            19224..19949
                     /locus_tag="VFC74_04910"
     CDS             19224..19949
                     /locus_tag="VFC74_04910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24711.1"
                     /translation="MTRLKVLVKSEFERLNKYNLFTANFVVLLLWMLLAWFLEGEVLL
                     QFMPIIFLTDSVMMTILLVGATLFYEKKEHTINSIMISPVTENEYLLAKVLISVGNSL
                     LTVLFISAAVYLMKGVRYDYLLLIPAVALVTVAHTLLGIRLSYSAKDFTSLLINFMVY
                     VFLFLLPSVFAALGIISSNIAQYFIILPPETSSILISAAFEKADLWKLFFSYAYLVLL
                     SLVLFQFVVKPKFNEYLMKETGV"
     gene            19952..20677
                     /locus_tag="VFC74_04915"
     CDS             19952..20677
                     /locus_tag="VFC74_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24712.1"
                     /translation="MLLTLTKFELKNLLRDKMTVFMIIYPLLLGGLGKYLIAHNYVDG
                     PALGITAMLIAILSGFAYGAMAGFSLLDDRDDHVFASLQIAPISLNSYIWFKVLFAYL
                     LAVLSNFFIVWFTAAFSLSPGNLLLISTLSALQTPIIAFIINAFAQNKVEGFVTMKAT
                     GLLLLLPIGSYFFLDAKEWLFAIAPGYWAAKAVQYSLLEPQIDAGLVSMNLNFYQYIL
                     LGIAYNLLLVIVAFGIFRRKNDI"
     gene            20698..20889
                     /locus_tag="VFC74_04920"
     CDS             20698..20889
                     /locus_tag="VFC74_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24713.1"
                     /translation="MSGISMIFLLIAVVYLFGTLFELPLVLENPKSKWIIDKIGKKQT
                     KILFIIIAVIFFILASKFK"
     gene            complement(20915..23251)
                     /locus_tag="VFC74_04925"
     CDS             complement(20915..23251)
                     /locus_tag="VFC74_04925"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010248936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HZK24714.1"
                     /translation="MGYKIKIAVKQLLEFVLRSGDIDRRFTGLERAVEGGRIHRRLQK
                     EAGEDYQSEVFLSTETACATFTITVEGRADGVFTRAESFWIDEIKSTMLPLAQIDETF
                     NSLHWAQAKVYAYIYCLQHELTSIGIQLTYVHAESNEVKRLQKIYSKEALTDYYFALL
                     AMFKKWADFGSQWAVTREHAIKKLQFPFDSYRHGQRKFAVAVYKTIASSGLLFAQAPT
                     GIGKTISTLFPAVKALGEGEVEKIFYLTAKTITRQVAAEAVATMRAQGLKLKTVTLTA
                     KDKACFLAERSCNPDSCRFAKGHFDRVNEALYQLLQEHDDFSREIIAVYAQKYTVCPF
                     ELALDLTLWSDCIICDYNYLFDPQVYLKRFFTSKGNYIFLVDEAHNLVDRAREMFSAT
                     LNKTAFLKLKKQIGKQEKELYRSLGKVNSVMVSLRKLCEQQEHFLLKELPAKLTKQLT
                     DFVLLCAAWLKDKQGQEIDEEFLQLYFDVLAYLKIAEFYDQCYVTMLRKNGSELQVKL
                     FCLDPSHLLQERLAKGKAAILFSATLTPFSYFIKVLGGNEEAKKLSLSSPYPQDQLCL
                     VLADRISTKYKNRAHSLAEIADLIYHTVSRKRGNYLVYFPSYKYLHDVYRIFAAQYAE
                     IQTQIQHSEMDEQAREAFLTAFDQDNVETLVGFCVLGGIYAEGIDLKGERLIGTIIVG
                     VGLPQLNTEHNIIRNYYQQQNQLGYEYAYLYPGMNKVLQAAGRVIRSETDKGIVLLID
                     ERFTQVTYRKLFPQHWQHYQVVRDASDLKLIMSDFWQK"
     gene            23518..25215
                     /locus_tag="VFC74_04930"
     CDS             23518..25215
                     /locus_tag="VFC74_04930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HZK24715.1"
                     /translation="MTEDLEVQSNFIYNNITKDVAEGKNEGKVHTRFPPEPNGYLHIG
                     HSKAILLNYRLAKNFNGLFNLRFDDTNPVKEEQEFVDSILEDLRWLGVEWGDRLFFTS
                     DSFELKYEFASRLIKAGRAYICDLSPAEIREYRGTLTEPGRESPYRSRSVEESLELFT
                     RMRQGEFANGERVLRAKIDMASGNLNMRDPVIYRILHASHHRTGDKWCIYPMYDFDHP
                     FTDALEGITHSLCSIEYTDHRPLYDWIVDNALALMPEHIKTRSQQTEFARLNMTYTVM
                     SKRKLRRLVEENYVAGWDDPRMPTIAGLRRRGVTAAAIADFQERVGVARADSTVDLAL
                     LEHCIREDLGPKAHRMMAVLEPLKVVITNWPADKTEILELENMPGDESAGVRQVALGR
                     EIYIEQEDFMEEPMKKFHRLAPGREVRLKGAYIIRCEEVIKDAAGQIMELHCTYDPQT
                     RSGEDTSGKKVKGVIHWVAVEHAAKVTVRLYDTLFIKENPEDETSGDFTRNINQDSLL
                     TITEVPVEPAAAEAAPGSRIQFMRKGYFYLDPEDAKAGQIVYNRTVELRDSWAKKKR"
     gene            complement(25268..26032)
                     /locus_tag="VFC74_04935"
     CDS             complement(25268..26032)
                     /locus_tag="VFC74_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HZK24716.1"
                     /translation="MEYTVRKIGKMAGVSARTLRYYDEIGILKPARINSSGYRIYGSA
                     EVDRLQQILFYRELGVCLEDIKAMITNPAFDANSALREHLAQLLHKQAQLETLIANVR
                     KTLALREGRITMSDQEKFAGFKQKLITENEQKYGKEVREKYGEEAVKQSNQAFKNMSP
                     EDLTRMQELEAEVRKSLAEAMPAGDPASPLAQKAAALHKQWITMAWGQYHKEAHAGVA
                     QMYVDDPRFTAYYDQEQPGTAVFLRDAVLIYTGMKK"
     gene            26220..26381
                     /locus_tag="VFC74_04940"
     CDS             26220..26381
                     /locus_tag="VFC74_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003355804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3787 domain-containing protein"
                     /protein_id="HZK24717.1"
                     /translation="MNKEKEKFMAKPIEKHDTAAWADREEIKRKSKVTIPHEVDVRNA
                     KEYVDSNQK"
     gene            26530..27762
                     /gene="pepT"
                     /locus_tag="VFC74_04945"
     CDS             26530..27762
                     /gene="pepT"
                     /locus_tag="VFC74_04945"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013726610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HZK24718.1"
                     /translation="MYKNLVPRFLRYLQKETRSDENSTTIPSTPSQKEFAQTLKIELQ
                     QLGLSEVTYNDHNGYLTARLPANTAAQVPTIGFIAHLDTADFNAKNINPQIHEHYHGE
                     AIVLNKDKNIVLSPDEFPNLKNYLGQTLITTDGTTLLGADDKAGIAAIITALEILSKD
                     DSIKHGEIRVAFGPDEEIGIGADRFDVQSFNADFAYTVDGGPVGELEYECFNAASALV
                     TIQGKNVHPGTAKDKMVNALKLALAFDASLPQAEVPEKTAGREGFYHLLRLTGEVEEA
                     KLSYIIRDHDRELFAKRKETILKIAQELNEKYGSERVVVAIKDSYYNMGEIIAKDMTV
                     VNLAQKAMEKLGIMPIIKPIRGGTDGSKITFMGLPTPNIFTGGENFHGRYEFITVESM
                     QKATAVIVEIARLNTESN"
     gene            complement(27759..29075)
                     /locus_tag="VFC74_04950"
     CDS             complement(27759..29075)
                     /locus_tag="VFC74_04950"
                     /inference="COORDINATES: protein motif:HMM:NF025774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MEDS domain-containing protein"
                     /protein_id="HZK24719.1"
                     /translation="MSQQSFISSNSITTVETHQLRAALLNLLRKSGIPYLQDIPWGTH
                     LCGFYQTDHDLLEIVVPYLRAGLENNEYCMWIVSGSITPSTAISVLKRLVPNFDLYQP
                     QLEILTHDQWYLKYGDLQVGKIEAGWQKKTEQALAKGYDGLRLCANTAWLTKKYWKKF
                     LDYEENVGKKIGKLKMIALCPYQLSACSMPQILDIVNSHHFSFIKSDYDDDCTNNIAK
                     YERINLVASMAASIAHEIRNPMTAVKGFIQLLQSKDEFTAYHDILALMVDEIERANGI
                     ISEYLSLARDKDKDIQRQDLNKILLAILPLLQADALREDKDVTLLAGEIVPLDIDTKD
                     IRQVVLNLTRNGLEAMPKGGILKIRTYMERDQVVLAIEDSGSGIPKEVMAKLGEPFVT
                     TKENGTGLGLPVSIKILDSYNATINIDTSAAGTTMAIFFPQENRSS"
     gene            complement(29354..30016)
                     /locus_tag="VFC74_04955"
     CDS             complement(29354..30016)
                     /locus_tag="VFC74_04955"
                     /EC_number="6.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphthine--ammonia ligase"
                     /protein_id="HZK24720.1"
                     /translation="MKIKNRPFFCSWSGGKDSCLSLYYAIQEGGIPKQLLTMLNEGGE
                     RSRSHGLSLALLQKQAALLEIPLTTEAATWGDYEANFITKLKQMKAAGIEIGVYGDID
                     LADHRQWVQRVSQVAGMLVYHPLWQKPRQQVLHEFITAGFKALVVSLDGKRLDKKYLG
                     QVVDQQLLAEFAAAGIDPAGENGEYHTVVIGGPIFKSDLQIILEEQLNQGDYWFQNIS
                     IK"
     gene            complement(30158..33853)
                     /locus_tag="VFC74_04960"
     CDS             complement(30158..33853)
                     /locus_tag="VFC74_04960"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HZK24721.1"
                     /translation="MLPKFSVEKRYTVFVAVILVLILGAVSFINLQTDLLPKIDLPYL
                     LVMTSYPGASPEEVEMVVTRPIEQVVATTNNIKNISSVSRENSSIVILEFNNDVNLDS
                     ATIEINGNLDLIKAAWSDSVGSSMIMRLNPEMLPIMVAAVDIEGKDTAEVSQIVRGEI
                     LPELESVNGVASVSGVGLLEEKIEVVISSEKITELNQKILANVDAELAQAERQLLAAR
                     KKIQSGKAQLAAENEKQSAKITAGAQAIAVALKQIEQAEVQMIAGEPQLEQTKKALQS
                     VLDVINYSEEQLIAQKEALLKQEELTEAEQTQLEFIAQSLTAVAAKKAETNNDLAVIT
                     AQTASLQEQKTLLTEQKQSLAEQEKQLQSGRQLLAAEMEKAKTELLSGEITLNQKITE
                     FERAKEEAFKNASLEGALTPKMIAGVLAAQNFAMPAGYLTESGTDYLVKVGDKLSEVD
                     ELTKLVLFDTGISGIEKIRLDDVAEIAVKDNADDTYAKVNGNNAVILTFQKQSNFSTA
                     AVSDSIEKKFAELLESNEKLNLTALMDQGVYIGIVIDSVLDNLIYGGLLAVLILWLFL
                     RDLKPTLIIALSIPISVVFAIAMMYFSGLTMNIISLAGLALGIGMLVDNSIVVIENIY
                     RLRNEGVPVIQAAIEGAKEVMGAIAASTFTTACVFLPIVFTQGITRQIFADMGLTIAY
                     SLFASLIVAITLVPTLASIMLNQTQEKEHHFFDGLLKVYERTLRWSLQHKAIVLVFVV
                     ALLGLSGFLALSMGTSFIPEMEAPQMSVAVTMPPEASFAEALAMSETVIERIDELAEI
                     ETIGAFQNSMMGGMGGLGSGGSGNSVSLYLVLKEDKARSNTEIAAEIRSLTADLECSI
                     TVTSSNMDISALGGAGLEVIIKGRELDTLREIAAEITQIMQATAGTVEVSDGMEQKST
                     ELRIAVNKEKAAEKGLTVAQVFASVNSLLADKNATTLSVANQDYPVIIIDGKSQTLTR
                     DNVADLVVAGSQNGESTEVKLADIAAINEAEGLSSITRDAQERYISVTASIDADHNIG
                     LVSRELEEKLQDYQLPSGYRVELAGETELINNSFVDLIYMLLLAIVFIYLIMVAQFQS
                     LLSPFIVMFTIPLAFTGGLLALAITGYELSLIAMLGFLVLSGVVVNNGIVFVDYTNQI
                     KGKGLSVKEALITAGKTRLRPILMTALTTILGLSTLSLGVGMGAETLQPLAITAIGGL
                     AYATVLTLLVVPVMYALFNKDQQDHQS"
     gene            complement(34073..35452)
                     /locus_tag="VFC74_04965"
     CDS             complement(34073..35452)
                     /locus_tag="VFC74_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24722.1"
                     /translation="MQKKPLSKAIKTYYGIGDLGFSLMTSVELFYFVYFLTDVAKYSL
                     AVVALIGSVTSIVDAVFSPFYGAIISGTKALKWGRNRSWLLIAPPAVVVLYAFQYTRI
                     GPEPIAAIIVCAGFILSHIIWNIAWVGNVALINVLANNPEERGLLASRRGTWTSLAGV
                     FFSYIGLPLAVMLGNLTGNEVMGYSLLAGVMAFTMLLGYWIVFKITDGYEETGEEADV
                     APGAQKVSLGIMVKSLIQNPPLMMLVVGDFFRYMVNFIMSAAAAYYFIYVAQNQALLP
                     VYLLTGAIAQVIGAYFSGNMARVLSTRSAAIVSLFGLAVSLFVTRLLAFNVTSFFIVV
                     LVARFFLGVLTAVMVALYTDAAVYGEWKTGENASPFVMGLMNISIKTAVISRGTLIPL
                     ILGLAGFIVGADPATATLELKTAVTNVFLLVPGIFALAAALIITFGYRLTREKLAEYQ
                     KEIDQRDAA"
     gene            complement(35652..38324)
                     /locus_tag="VFC74_04970"
     CDS             complement(35652..38324)
                     /locus_tag="VFC74_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003356219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HZK24723.1"
                     /translation="MWFAKSQEEVLKEFAVDPAAGLSSEEAQERLNKYGENKLKSKPK
                     KSLIVLFFAQLQDMLIYVLLGATAITLVIQEYVDAVIILLVVILNAVIGVVQEYKAEK
                     AIEALQKMTTPKAIVRRDGEVKEIDSEQVVPGDIVIIDAGRYIPADLRLIDSANLQIE
                     ESALTGESVPADKNAEKLHVNPKTPIGDKSNMSFMSTLATYGRGEGVAIATGMETEVG
                     KIAKILDEDHEELTPLQKRLEELGKILGFIAIGICALIFVIALFQKRDLFEMFLTAIS
                     LAVAAIPEGLAAIVAIVLALGVTKMSKINAIVKKLPAVETLGSVNIICSDKTGTLTQN
                     KMTVVKTYTANNLADVPKTGSDFTITNVDKINLMKTFVLCSDATYENGESTGDPTEIA
                     LVVLGERFNLAKVKLNSQHQRVAEKPFDSNRKLMSTLNKEGDGFRVHTKGAIDNIINL
                     ATTALVDGKVVPLTDSMKEKYLKIADEMSNNALRVLGAAYKDTTVLITPEEMEEELTL
                     TGLVGMIDPPRLEVKDSIREAKLAGITPIMITGDHRNTAVAIAKELGIADGIEQSLLG
                     AEIDEMSDEEFSEQISNYRVFARVSPEHKVKIVRAYKAHGNIVSMTGDGVNDAPSLKY
                     ADIGVAMGITGTDVSKGASDMILTDDNFTTIVRAIEEGRNIYNNIKKSVIFLLSCNLG
                     EVIAVFASILFFWPVPLLPTQILWINLITDSLPAIALGVDPGDKDVMKKQPRNPKESF
                     FANGAGTRSIIGGTLIGTLTLLAFYFGLSEHGYSLGSTDIPETVLTYARTMSFVVLAA
                     SQLFYSLTVRSSSKSIFQIGIFSNIYLIGAIIVGFILQFGVISVPFLANAFKVHNLSF
                     RDWGLVFAFSLLPLIVNEIIKKITNSTQSKESTT"
     gene            complement(38664..39005)
                     /locus_tag="VFC74_04975"
     CDS             complement(38664..39005)
                     /locus_tag="VFC74_04975"
                     /inference="COORDINATES: protein motif:HMM:NF013844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HZK24724.1"
                     /translation="MRKKVAQVNLEEGLPTVEAAIQRMKNALTTAKRQGYKAVILIHG
                     YGSTGVGGKIKPAVAKVLSEHSLKGIVRTYAGGEQWIDRKREMIGMCKALENFNFKVA
                     NNAGVTVVILR"
     gene            39270..39806
                     /locus_tag="VFC74_04980"
     CDS             39270..39806
                     /locus_tag="VFC74_04980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24725.1"
                     /translation="MKKASCILLVLVSLFVLVGCSGGKTPANADPIKMGRVEHAAHGT
                     KCFAVAVVAVQGDTIVGASLDEYQFMSTDVATGVPNADADFAKNFADPAVVLASKRAN
                     ADYYSEHMTEAAGATKRLDEQYDTIEAYVVGKTVAELESTLSENDGAGMIDAVSSVTL
                     VDTKGYVEALVAAAKAAK"
     gene            complement(39892..40071)
                     /locus_tag="VFC74_04985"
     CDS             complement(39892..40071)
                     /locus_tag="VFC74_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK24726.1"
                     /translation="MDSYAVVLDFFNKADKPASAGEIATATGVDRKEVDKVMKKLKKE
                     EKIVSPTRCYWEISK"
     gene            complement(40190..41026)
                     /locus_tag="VFC74_04990"
     CDS             complement(40190..41026)
                     /locus_tag="VFC74_04990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZK24727.1"
                     /translation="MSESCNENCSSCGEDCAERKEAQPDFSAKPHKLSKIKKVIGVLS
                     GKGGVGKSLVTSLLAVMMNRKGYHSAILDADVTGPSIPKAFGLKKKATGSEEGIYPVQ
                     SKTGIDIISINLLLPNDTDPVVWRGPIIAGAVKQFWTDVIWQDVDFMFIDMPPGTGDV
                     PLTVFQSIPIDGIIIVTSPQELVSMIVSKAAKMAEMMHIPILGLVENMSYFQCPECKE
                     NHKIFGDSHLEETAQQHNIDLLAKLPIDPKLAYAVDRGQIELFAGDWLEPLADYLEKK
                     LV"
     gene            complement(41050..41409)
                     /locus_tag="VFC74_04995"
     CDS             complement(41050..41409)
                     /locus_tag="VFC74_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HZK24728.1"
                     /translation="MARPRKWRKVCCLPESNLFGPLNSVSTDQEVIIMTVEEYESVRL
                     IDLEGLKQEECATRMNVARTTVQRIYNEARKKIAEALVSGTILKIEGGDYKLYDEGER
                     GRGCGRCRRRRFGQHGK"
     gene            complement(41479..41748)
                     /locus_tag="VFC74_05000"
     CDS             complement(41479..41748)
                     /locus_tag="VFC74_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5320 domain-containing protein"
                     /protein_id="HZK24729.1"
                     /translation="MPRMDGTGPMGAGSFTGGGFGPCVKANTNFVGGYGRGARGCGRG
                     YGRGFIAEPADFPARKELLAEKKEILERRLKMISSQLSELEPKDK"
     gene            complement(41890..44238)
                     /locus_tag="VFC74_05005"
     CDS             complement(41890..44238)
                     /locus_tag="VFC74_05005"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HZK24730.1"
                     /translation="MQLTSTAKVVLEKRYLQKDAEQIVETPEDMLYRVAAVVAQIEQQ
                     YAKTSQEIKTLTKQFYEMMANLEFMPNSPTLMNAGRDLGQLSACFVLPVEDSMEAIFD
                     AIKFAAIIHKSGGGTGFSFSRLRPKNSSVRSTGGVASGPVSFMKVFNAATEAVKQGGT
                     RRGANMGILRVDHPDIMEFIQCKEDNQEITNFNISVGITEEFMEALKEQQNYDLVDPH
                     TKEVVAQLSAAEVFSKIVDHAWRNGEPGVVFLERLNKDNPTPKLGDIEATNPCGEQPL
                     LPNEACNLGSINLKGMVKEQEEGAAIDWEHLAEITRLAVRFLDNVIDANQYPLENIEK
                     VVKGNRKIGLGVMGFADLLAQLQIPYASAEAVEAAEKIMKFIQLQGYLESERLANERG
                     TFPNYEGSIYDGERKMRNATITTIAPTGTISMICGASSGVEPIFAVAYTKNVLDGNSF
                     VEVNPLFEGYAQEYGFASPQLMAKIAETGSVQGLDEVPAWVQEAFLTASEIPSEWHIQ
                     IQAAFQKYTDNAVSKTINFANAATREDIARAFLLAYELNCKGLTVYRDGSRDEQVLSF
                     GTAKEQPAATAPAAQVQAPNSITPRPRPEVTMGVTEKIKIGCGNLYVSVNADEQGICE
                     IFTNTGRAGGCASQSEATARLISIALRSGISVNAILNQIKGIRCAACIRREGVNVTSC
                     PDAIARVIKKYMDVGGTENGQAIQSSTAKANGNGLSKSLQKLSDVAAKSGEYVKKIPV
                     NGMKKKASTGVAIDLACPDCGMPINHESGCVVCIHCGYSKCG"
     gene            complement(44440..45237)
                     /gene="zupT"
                     /locus_tag="VFC74_05010"
     CDS             complement(44440..45237)
                     /gene="zupT"
                     /locus_tag="VFC74_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003438017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HZK24731.1"
                     /translation="MPENNNLLFAFGITLFAGLSTVVGSLFACYTTQTNKKFLSAALG
                     VSAGVMIYVSMIEIFPEARASLTLIYGAKKGYWVTTLAFFAGIALIAIIDLLVPSADN
                     PHEIQDVDDSANDANLLRIGLFSALAVAIHNFPEGLATFVSAIQDPKLGLSIAIAIAI
                     HNIPEGIAISCPVYFATGDRKKAVLYSLLSGFAEPIGAVVGYFLLLNFLSDAVFGITF
                     AGVAGIMVYISLDELLPTAEKYGEHHIAILGLVSGMAVMALSLLLFS"
     gene            complement(45368..46258)
                     /locus_tag="VFC74_05015"
     CDS             complement(45368..46258)
                     /locus_tag="VFC74_05015"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK24732.1"
                     /translation="MRKPRIHYYGALYHVMVRGNNGENIFLNDKNKRKYLDLLAQYKN
                     ALGFSIYSFCIMDNHAHLLIEVQDVTLAKIMQRIQQVYTQWFNYKYERTGHVFQQRYK
                     AQLCNKEKYLLQLIRYIHNNPVKTHLPGGVEYKWSSHLNYLGKGSLDLVDTSFILQLF
                     SDNAKQALKEYMYFMNQEPDIQSAESMYEVKPKVLKEKSKKATSGSEIGIDELIKEVC
                     RLEQIKYEQLIKKTRQQKISDLRKAVVLLSEKQCLVKNATLAQKLNLSPSMISKIKSG
                     EVEITAYVREVMQKWLEISE"
     gene            46435..46746
                     /locus_tag="VFC74_05020"
     CDS             46435..46746
                     /locus_tag="VFC74_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017212798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin"
                     /protein_id="HZK24733.1"
                     /translation="MKVIMYSSDICPDCVDAKKQLAALPKLELDYRSITESTTRLKEF
                     LSFRDHEEMFVSIKEHGKIGIPFFILADGTKTFDVGDFVQIDRPTSSASSCSLDGEKQ
                     C"
BASE COUNT        13280 a        10880 c         9101 g        13632 t
ORIGIN      
        1 gtttaatttt aactataagg aggcaacgac taacgtttct gaaaaaactc tcgcgttagt
       61 ttcacgataa ggccatttaa tccccataag cctattaaat tggggatagc catcaagcca
      121 tttaaagtgt cagccagagc ccaaactaca tgcagccctc ctacagcgcc gatgggaatt
      181 agagcaatcc acaacagacg atagaaaagt gtcgcacgtg tactaacaaa atattcaaag
      241 catttttcac cgtaatagga ccaggaaatc aaagtagaaa acgcaaaaat caccaagcta
      301 acagcgacaa aataaccacc ccagctggga agtgcctgat tgaaggctgc tgtagtcatg
      361 gctgcaccat caagaccagt gttccaaacc cctgttacca gcacggctag ggctgttaag
      421 gtgcaagaga tatgggtatc aaaaaacact tcaaacatac cccacaaacc ctgacgtgct
      481 gggtgatcag tgcgggccgc cgcatgggca atggaggcac tgcctaaccc tgcttcattg
      541 gtaaagatcc cacgggccac accgaaacga aaagtctgca aaacagtagc gccagcaaag
      601 ccgcctactg ctgcccgacc ggtaaaagca ttgccgatta tttgactaaa agctaaaggt
      661 acttgtgcca agtttaaaaa gataataaac aacgcgcctg ctgtataaaa agcagccatc
      721 aatggcacca atttttcggt aaaggccgct acacggctga tgccgccaat aattaccaag
      781 gcagtaatag ctgccatgac tatcccggtt attaattgag gtacattaaa agcctcgtgg
      841 agcgcggccg ctacagagtt agcctgcacc atattaccta tgccgaaggc ggcaagtgag
      901 ccaaataaag caaatagtgc agccagcaga cgactgttca gacctttcgc caagaaatac
      961 attggacccc cgaccaccct gcctgttggt tgggtatcac gaaagtgcac agccaacacc
     1021 acttcagcgt atttagtgac catccccaca aaagcagata cccacatcca aaagacagca
     1081 ccaggtccgc ctaaagtaat agctgtggcc acgccagcga tattgccggt acctatggtg
     1141 cctgccaacg ctgtcgtcaa cgcttgaaat gcactaattt caccaatacc agtattcttt
     1201 tggcgcctca gaccgaagat agtatcacgc agcatagtac tgaaatggcg tacctgcagc
     1261 caatttaaac gtagagttaa gaaacaacca gtcaacagaa cggcggcaat cataatgggg
     1321 ccgccgacaa attcctgtaa aaaaaagttt agtgactgta tactcacaac tcactcccct
     1381 ttgccacatc tatttgataa gtatgcctta aacctgtgat ccatccgctt ctttatttaa
     1441 taataacgtt gtacacgaaa acgatagagt tggtacagct cgctagggtt aatgcctgct
     1501 ttctgtaagg ctatcgacac ttggacttca gctgtctcga caccagacaa atttggtaag
     1561 agcaaccctc tacgctgccc ctcttccaca attacaccaa actgctgtgg atcaagttct
     1621 gccatactat ctacagcttc cgccgcggcc agcacatcaa cagagtaaac taaaccagaa
     1681 agctcctttg ctgtgacggg agggaaacgt gggtcataga tgccggcgga aatggcattt
     1741 tcaacaattt ccgccgctaa atttgcttgc tgcgcctcaa tgctgccgat acaaccacgt
     1801 agttctccat ttaaatggag tgagacaaaa gctccagcac gttgttgaca gagcgaggaa
     1861 gcagtgggtg gggtaatgac ttgcttggta cgcacaaaag tttctagtgt gtgtctagcc
     1921 aactggacat gttcactctc ttgtgttctg gtagaagcaa agggctgcag cgttcttttc
     1981 ctgccaggcg tcaaatccgc caccaagtaa cccacgccaa aggggccttc gtaggaaaga
     2041 atttctggct cgacagtttc tccatctaag atacccaaag caattatgag ggaacgataa
     2101 ccgcattccc ctgcctcttc tactaaacgg tgattaaggt taagaatggc ttcaacatca
     2161 aatctctcca ctaaatgaat tagcttttga tcgaattctt gccctgcagg agaatagcca
     2221 gccggggcat cttcctttaa acggtgcgaa agatctgcac tgcaaatcac agcagcatgc
     2281 cgcttgagtc gcagcaaagc cttgcggact gcctgcccaa agctgtatag ctgtttcgca
     2341 gcaagcagtc catatgtgat atgcaccagc ggcaggcgga tcccccgctg ctgcaagaaa
     2401 tataatggca ccatcgcacc atgatccaag gtcacttcaa caccataagc tgcactgcgg
     2461 cggttatcta aatgagcaca gttgatacca aaccgctcag cctccagttc tatggcctgc
     2521 agcacactct tttcattagg ccaggaaaaa gtaatttcgg gtgcagcaaa ctgtgccagt
     2581 gaacctgtta attgctcatc tgctaatagt gctacggcat cacgaaacat aggcccatgt
     2641 ggcgtaatga ggactaaaac atcagcgccg ctttgtttaa tacgccccgc cagctcgctc
     2701 atggctgcag cggtctctgc aaccagtttt gcttcacttc cccctactgc cggaattacg
     2761 attgctgggt gagggtaaat accagcatgc acaactgttc tcattgacct actcctttat
     2821 ctccgtttac ttctatttta accggaaatc ttttccttta ctacttctaa cttcatcgat
     2881 acattgcaac ttgcagaaga tttaggagga gttctgctaa ataacaggat tttgtcaaag
     2941 caatttagaa tacttattaa tcgactactg caggaggttg tgcatagtaa tgaatcagac
     3001 ccaaataaag agcccacgct actttattct gataggccgg atctgtgagt agttgtgctt
     3061 cttcagggtt agagataaaa ccacactcta ccaaagcggc acaacgctct gtttcgttga
     3121 gcacaaagta attacctggt ttgattactc tgtcggtgtt gcctaaaaca cgaactagtt
     3181 cctgctgtaa tgcttgagca agtattatag actgctgatg ttctattttg tgaaaaactt
     3241 gcgcaccacg ccattgggct gagccgatag cattagcatg aatgctcaaa aaaatgtcag
     3301 ggtcagcagc ctctacaatt tgcattctat ttctcatatc ttcttgcttt ttgggacctg
     3361 cagtaggctg caccagtaaa tccgtgtctg tttcccgagt catcactact cgcgctcctg
     3421 aagcttgtaa atagtcacgt aactgtaagg caattgccaa cactatgttt ttttcctcaa
     3481 cattgttgtt cctgacaccg ggatcataac caccgtggcc gggatcaagc acaatggttt
     3541 tattagctaa aggtaaacaa ccttggacag taatggcctt actaaccggc aggacaatct
     3601 tgataaccga tactaatatt agcaaacaca gtaaatatac gcagattaat aaatatttac
     3661 gcctagtcct ctgactgcca ataaaaaaaa tacgcaagac agacccccct ttagcaaaag
     3721 atatgaaaaa taaacgacgc ttatgaggtg aggcttgtat gaaaaacagt agtttactgc
     3781 aattattcct aacctttttt aagattggtg ctactacctt tggtggtggc tttgccatgc
     3841 tgccaattat taaaagagaa gtagtagaaa ggtacgcttg gttatcggat gaagaatttg
     3901 ttgatacgtt ggcagtaaca caatcactac ctggagcaat tgctgttaat tcagcaattt
     3961 tcatcggcta cagagtccgg ggctatgctg gtgcactact ttccatgttt ggagcagttc
     4021 tgccctcgtt tctagtcatt ctgttcatag ctaccttttt tgcccgtttt gcagaatacc
     4081 ccatggtgca ggctgctttt gccggtattc gtccagccat tgccgccctt attgcggccg
     4141 ccgtgctgaa agttggcaag ccagttttaa aaaattgtca aagcataatt ttcgctgccg
     4201 catttctctt actttccgag gggttaggcc tccatgccat aatagttatc ctcttgggcg
     4261 cactcagtgg cctgttaatg cactatctaa agacaaaaaa aaattgtgag gttgatgcac
     4321 aatgatctta ctgcagctct accttgcttt tctgaaaatg ggtttcttta gtatcggcgg
     4381 cggttatgtc atgctctccc taatcgaaac agaaatcatt accattcgag gctggttgtc
     4441 attacctgaa ttcgtcgaca ttttagccat ctccgaaatg actcctggcc ccattgccat
     4501 taattctgca acttttatcg gctaccgtat cgcaggcttc tggggttcag ccgttgccac
     4561 agccggcgtg attacaccct ctattatttt agtcctcttg gctgctactt tcctcaaacg
     4621 cttctatcaa aatcaatggg tgcaagctgc attttctggg ttacgtcccg ccgtcatcgg
     4681 cctgattgcc ggcgccgcca tctttgtaac ccgtaccgct ttggtcgatt taccatctgt
     4741 aggcatagcc ctaggcgcgt ttattctact ggtttttaca cgtttgcacc caattctcgt
     4801 cctagcactt tctgcattag cagggattct cataaatcta tagcatgtaa ctttcggtca
     4861 gccgtaagct gtaaatacta aaacccccaa aggcttaatg cctttggggg ttttccatta
     4921 actcacgtat ttttttaatc ggcacaccgc cagccatagt ttgcggggca acatccttgt
     4981 tcaccaaaga atgcgcagaa accatcgccc tctcccctac cgttacaccg gcaagcacgg
     5041 tggagttggc accaattagc acatttgccc ctattttaac ttcaccagtt gcccattcat
     5101 ctactaaaaa ctcatgcgct aaaaaggtag cattatagcc gatgacactg ttatcgccaa
     5161 tggtgatcag ctgcggaaaa aagacatcaa acatcaccat caaacctaca gaaacatcct
     5221 tacctatttt tatccctact aaacgataaa gtacattttt caaacgcaac gaaggtaaat
     5281 aacggcacaa atagataact ataaaattaa aaaccacacg ccacggttta acatagcttg
     5341 tccagtaata aagtgaattt ttatcacccg gaacatgatg tttagtaagc ttacgcatct
     5401 cactgccacc gcccacaagt aaatttaagc tactgtaatt atctttttgc tataccagct
     5461 tgtatatgca ttttggcctg cctactttgc aaaaagcagc tgccttttct agcttcgccc
     5521 ttaacgggct gccgttgaaa tttttaaaag ttgccatttt ttgtttgctg ttttttcctt
     5581 agcagtgtat aatatggtta ccactgcatt ttccttcggg tttgggaatc tagagctcct
     5641 ttgtttggtg ttagatttct cagtaagtac cagggttggg gaaatcaggg gtccaaaaca
     5701 aaggaggttt tttcatgtac gaggacaaga ctttaacctg tcgtgaatgc caagaagaat
     5761 ttatcttcac agttggggaa caggaatttt atgccgagaa agggttcgag aatgagccgg
     5821 ctcgctgccc atcctgccgc gctgctcgca aacagcgcat gaatggtgac cgctctgaac
     5881 gccgtatgta caaagttacc tgttctgaat gcggtgtcga aacagaagtt ccttttaatc
     5941 cttccctaga ccgcccagtt tactgtcgtg attgtttcca aaagaataaa agcttctaag
     6001 tgaacgctta actcgttagt ttaaattctc tctcgtcagg caagacgaga gagttttatt
     6061 ttacgctaat cttatctggt caacagcatc acctaaggtg gcagcataca tccgccgcac
     6121 caaattgccg ttttatttag tgttttatgc aggaattata cttttacaac agaatttaac
     6181 agcatacact agtagtaagg tactagagat aaggagtggt gaaatgcagt ttgataagtc
     6241 caaatcattg ttaggcatag tcgtgattac cgctggtatc atcttacttc ttgataatct
     6301 taatatcatt gcaggtatca acctttttgc ctattggcca gtgctcttaa ttgtctgggg
     6361 actgcaatct ttatggaccg gcagcaaaaa tcctgcagca caaggcattt ccgcttttgt
     6421 tattatccta ggtttcatat ttctagccaa tagtttgacc tgggctgaga ttaatgtcgg
     6481 cagagttttt cagtttttct tccccgtttt aatcattctc ataggcggca gcatttttct
     6541 tggtcgctcc tttgccggga aagccaactt ggccatcatg ggggcagtag agcgaggcaa
     6601 aacttcttcc tggcagttag aatcaggctc ttatgtggcc tttttaggag gaattgacct
     6661 agatttacgt catgccataa ttccagaagg agaaactgtt ttggatttaa cggcagtaat
     6721 gggtggaatc gatatccgcg ttcccgctga cataccggta gtagccgacg gctttgctgt
     6781 actcggcgga gttgagtttt ttggtaaagg cagtgggggc attgtcggct ccacacatac
     6841 agaacaaaat attggtgaag gcaaccaagt cctcataatt caggctcgcg ccgtgatggg
     6901 tggcatagat ataaaacgtg tatagcatcg gtctgcccaa agtgaatcgt acaatcttga
     6961 caaaacttgg atttaaaaat tcccttgcag ttcatttagg ctgcaaggga gttttgctta
     7021 aaatggtaat gggaggtttc ctgtaaaata gagaaaggtt aggatcccca ggatcgccag
     7081 tagggcgatg cgcaaggcaa cttttagtac tgtttttaag ataaaaactg ccacaacgat
     7141 taccacacct aaaataacta tactgcttat tccttccaca gcttatccac ccctctctaa
     7201 tctcccttaa gttaacatat gcggttagct gattattgat gtcaagaaag agacaaactc
     7261 ttcctgcttt aatcagttga taggtttttt tagccagtct gaccaatagc agctaaatca
     7321 ctaaattggc tgttataaat gtcagcgtag aagccgtcca tctccaggag ttcggcatta
     7381 gtaccttttt ccacaacgtc cccattatcc attaccaaaa ttaagtctgc atcacgaact
     7441 gtagaaagac ggtgagcaat aacaaaattg gtacgacctt tcattaactg acccattgct
     7501 ttctggataa gaatttctgt ccgtgtatca acactactgg ttgcttcatc cagaatcaaa
     7561 atatctggat cggccaaaat tgcacgggca atggtcagta attgtctttg tccttgtgag
     7621 atattggacg cttcctcatt taagatggta tcataacctt ccggcagggt ccggataaag
     7681 tggtcagcat gagctgcttt cgcggcctta acaatttctt cttctgttgc tccttctcga
     7741 ccgtaagcaa tattatcatg aattgtgcct ttaaagagcc aagtatcctg gagcaccata
     7801 ccaaaaatac tgcgtagtgt actacgttta atgtcacgca gctcaatgcc gtcaatagta
     7861 atcctgcctt cattgacctc atagaaacgc attagcagat taactagtgt tgttttccct
     7921 gctccagtag gacctacaat ggcgatagtt tgaccagatt ggacatcaat gttcatatcc
     7981 ttcatgagga tgctgtcttc ggcataacca aatttgacat gctcaaaacg cacattcccc
     8041 ttaggcacaa attcagccag cgcctgctca ccatcagcta cttcttcctc ttcatccagc
     8101 agctcaaaga cacgttcagc ggccgcaatg gttgattgaa agatattggc aatgttggcg
     8161 gcgttagtaa tgggttgact gaattggcga gagtattgga aaaatgcctg cacattacca
     8221 atggtaattg caccttgtgt aacaaaaata ctacccacaa cagctaccag gacgtaagcg
     8281 atattgttta caaagccaat gagcggcatg ataataccgg agacaaactg tgctttccac
     8341 ccaacatcat acaagcgctc attaatatca ttaaattcag ctacagattt atgttcgtgg
     8401 ttaaaagcct ttactattag gtgcccacca tacatttctt ccacatggcc attcatattc
     8461 cctaattctc tctgctgggc agcaaagaac ttttgtgact gtcgcgcaac aagcgtggta
     8521 ataataatgg acaaaggaat agaaactaaa gtaatcaacg ttaaccagcc gctaattgtg
     8581 agcatcataa ataacacacc gaaaaaagtt accacagact cgattacttg ggccaaactt
     8641 tgctgcagag tcctggcaat gagatccatg tcattagtta cacgacttag aatttcaccg
     8701 tgtgtttgag catcataata cttaaagggc aaccgtgaca ttttttcact tacttcaaga
     8761 cgcatatcat agactgtttt ttgggaaacg ctcgccatga tgtaatgctc aaaatatctg
     8821 aaggcagctg cagtaatata gagaccaata agcatcagta agatctgacg aatacgcgca
     8881 aaatcaatcc cgccaccact gacattatta actctatcca tcataccttc gaagatcaag
     8941 gttgtggcct gccctaaaat tttaggacta acggtagtaa aaacagtgcc gagtacagct
     9001 gctataaata ccaagagcag cgttaaccag tgtggtttta aatattttat caagcgtcca
     9061 gtagtctttt taaagttttt tggtttttct gtcggcattc caacccgtcc gccaggacca
     9121 tgaccacccc ctggtccgcg tcgtgggcca cgccccggac caccagcggg tggagtacca
     9181 ggagggccgc ctggacgaga agcggcgttt tgggaacgat tactattact catgctattt
     9241 cctcctctgt aagctgtgaa gaaacaatct cctggtaaac aggattgctg gccattaatt
     9301 ctctatgtgt gcccatgcca accacttcac catcatcgag cacaataata cgatcagcat
     9361 ccataacagt actaacacgc tgtgctacga ttaaaacaga agcttcagtt gtctcacttt
     9421 tgagagcagc gcgcagcctg gcatcggtct taaagtcaag tgcagaaaag ctatcgtcaa
     9481 agatatagat ctccggtttc cgtgctaacg ctctagcaat ggaaagacgc tgtttctgtc
     9541 cgccagaaac gttggcaccg ccctgagcaa tcattgaatc aaaaccatcg ggcattgact
     9601 ttacaaaatc actagcctgt gctatttcta cagcatgttc tatttccgca tcagtagctt
     9661 cttcctgacc aaaacgaata ttttcagcta cagaaccgga gaaaagtacc gcttgttgtg
     9721 gcacataacc gattttttca cgcagctgta cttgtgacat ctcacgaata tccacgtcat
     9781 tgatcagaat actgccacta tcgagctcgt aaaaacgcag caaaatacta gacatcgttg
     9841 atttaccaga gcctgtgccg ccaataatgg ctgttatctc accaggcccg gcggtgaagt
     9901 taatgtttcg taaggctgga cgctcggctc ctggatagct aaatgtaaca tccttgaatt
     9961 ctacccgtcc gtgcgcttcc tccgccgttt tctccacact cgggtcacta atactgggca
    10021 ctgtttccag tacttcatta atccggttgg ctgaggcagc agcgcgcggg atcatcacaa
    10081 aaatcatgga aagactaaac atcgaaaaca taatatgcgt tccatactgg ataaatgcca
    10141 ttaaatcacc tgtcatcatc tcgccataac tgatacgaat gcctccaaac caaacaatag
    10201 caatcgtggt caagttcatg accatcatca tcccaggcat catcagcgac atgatccgat
    10261 ggacgcgaat agaagtattg gtataatcaa ggtttgccgt ctcaaaacgt tttttctcgt
    10321 agtcctcacg actaaaagct cgtatgacac gcataccagt cagtctttcc cgcagaacca
    10381 gatttagagt gtctagttta gtctggatag cgcgaaaaag aggtgaccct ttacgtccaa
    10441 caaaagttat aaagccgaag agtaaaggca gcgccacgac aaaaatcagg gagagtttag
    10501 catctttaga aactgccaaa ataagaccac caatactcat cacaggcgcc attaccatca
    10561 tcctgatggc cataacgagc atcatctgga tctgtgtaac atcgtttgtg gtacgagtaa
    10621 tcagagaagc agtaccgaat ttatcgaact catgcagtga aaagttttcc acatgggcaa
    10681 atactttggt gcgcaaatta cgcgcaaagc ccatcgctga cttggaggcg aaatagttgg
    10741 aagctactgc acagatgccc ccaaaggcgg caatagctaa cattaaacca ccaatgcgcg
    10801 caataatctg agtatcacca ttaacaatcc ctttattaac aatatccgct gttaaagttg
    10861 gcagaaataa gtcagttaaa gcacgtacaa ctaccaagat tagcgctata gctacctgaa
    10921 atttaaaggg ctttaactca cgtaaaattt ttaacacgta cgtatcactc cgtttcacca
    10981 gcaaatttag taggtgtact attgcgtaga tagtcaaaac attctaccag caattcaatt
    11041 aactgctgac ttttttcttc acctaaatgc ttaaccaagc cataaaactc tgagaaaaat
    11101 tcatcagcag ctgcttgtat ggtttcctct cccttcgttg tcagtacgac acgaatggag
    11161 cgccgatctt ggggatcagt tgttcgttct accaacccgc tgctttctaa actattcacg
    11221 agctgcgtaa ccgttggtga ggttacacgc ataacatggc ttaaatcaga gatgcgcgca
    11281 ccgcagccct gcatagttag actttttttc acgccaaaaa ggatacgcaa ctcactgttc
    11341 tttagtcctg gaataggaca ccctcgatgc agacgcctaa agcgcgaaaa taaatccatc
    11401 agtttctgtg tagtttctcg gtccgcatta tccatttagt ccccccctcg agtaaattta
    11461 tcgccaaaaa tgagtactga ctacctcctc tacatttttt ctcccagaca aaaatataat
    11521 taggtaggta gcctacctaa ttatatttga tttcattttg aattgcaact gctttttttt
    11581 aaattgtgaa aaaagtttta agctttacta tcttcaacct gattgcagta aatcctcttc
    11641 ttttagcacg ggaaaaatca gttctacctc aaataaatca ccgacgatct tgatgttaaa
    11701 cttaccaccc tgtatttctg ctaaacttct agcaatggaa agcccgagcc cgcttccttc
    11761 agtatgacgt gctttatcac cacgcacaaa acgctccatt aattcttccg gtgagatatt
    11821 taatttctct gcagagatgt ttttgatgat taatttcatt tcagtagctg tggcttcaag
    11881 atcaagatag acacgcgtct gaggctgggc atatttttta acattagaaa aaagattttc
    11941 taataagcgg ctcaaatgac ggcgatctcc gttgatgaca actttttctc ctggtactgt
    12001 ctgaattact tctagccccc gctcacgcag acgttcgtca tattcaccgg taatctgctt
    12061 cagcagttca ttgacattaa tctctgtcaa atctacagta agattacctg tgctggcctt
    12121 tgaagcttcc attaaatctt caattaaatc ttttaagcga taagattttt gctcaaggac
    12181 atctaaataa gcaagcgcct gctcattttc taaggctgtt ttcttcaaga ggtctacata
    12241 attaataatg gaagtcagcg gtgttttaat atcatgtgag acattagtga tcagttctgt
    12301 ctttaatctc tcactgctca ttctttcttc cacagccttt tgtaaacctg cactaatggc
    12361 atttaaggct tgtgcctgtt ctttaaaagt gagggtaaat ttggtgctgt ccaagtttac
    12421 tttcagctgc ccggcagcaa ttttctgcat gttttgttta agctgtgaga cctgcagcgc
    12481 cagataaaga ataaagagca gcacggccga attaaacatg agaaaaaaga gcattgccaa
    12541 accactatgc atattaaaaa taaccgtgat attaatcaga ccaaaaataa ttacggctac
    12601 tcctgcctga tatacttcag tcaaatgaag aaaaattttc ctcaacaggt cataaagacg
    12661 ataaaaaagg gtgttggtta ataatgtctg tgtccttaac cttttaacaa tggtaataca
    12721 ccacagcata aagatgctgt agagataaaa aagaattaaa cttaagtaga taccaagccc
    12781 cacatctgtg cttaggtcat agtagctggt gatggtgcct atgccaagtc cgccagtaat
    12841 gggcaggatg attacaccca gtaaaaataa agttccacag ccaagagaga ttaaatctgt
    12901 tggaatgcga tcccatctat ttaggacaag ttcttcactg ttcctttgag ttcctgctgc
    12961 tgccaagaga aaggaaaaga gcaaaatact tacgacaatg gttaagggca gcataatacc
    13021 cgcgctagtt aagaaccagg agaaaggcca gatattgcgc actactaatt cttcaatcct
    13081 tcctcccacc acgaagttat aagcagagat cagtaggatg taaccgttca gatagagtaa
    13141 gcagaaggcg ctgatcttgg cggttaagct atactttagc tctttcactt ttataacctc
    13201 tctatcttat accccaaacc ccagacaacc ttaatataga tggggttctt gggatcgatc
    13261 tcaatctttt cccgtaaatg gcgaatatgc acagacacca aattatcaga agcaacaggt
    13321 gcttcctgcc aaactttctc atagatctgg tcagtagaaa ataccctgcc cttgttgata
    13381 agcaaaagtt ttaagatatt gtattcaagg ggtgtcaagt tcactggttt gtcatctaca
    13441 gtcaccagct tttgttgatc atccaaaact agaccgcctg aagcgtagac atccgctttg
    13501 acttctcggc tgccaaggga agcatatctg cgtagttgtg cttttactct ggcaattaac
    13561 tccagtgggt taaaaggttt ggttagataa tcatcagcac ccatatttaa gcctaaaatc
    13621 ttatcagtat cctcactttt agcactcaac ataataatgg ggatattttt atcttcacgg
    13681 atttttactg tagcgcggat gccgtccatt tctggcatca tgatatccat aataactaaa
    13741 tctacttgtt cgctgttaag aattgctaaa gcttctctgc cattgtaagc tgtcttaatc
    13801 tgatagcctt catttgctag atagatctca atggcggcga caatctcttt atcatcatca
    13861 cacaccaaaa tattaaactg acccatcaca actacaccgc cccttcatca atatcaccta
    13921 tttcgacagt gactatataa tgacctgtgc catcacaaga aagttgatag tttaaatcct
    13981 ctggtatctc aatacaattt tctcgggtct tcacacgata gacgagatag ttttgtgtct
    14041 cagtcttaat ttggtagaca gtcccagggt taagtgccgc caggtattcc tccagacaga
    14101 gcttgctttc cgccatcacc gccgcatgag gtatacctac atagcgaaag tgccacggtt
    14161 cataagggat ccccgtaatc tccgcctttt cctgcggata gcgcataata aagccgtact
    14221 gatgagcatg ttcaactaac caggcaccct gccggctaga caaaaaggaa ttaaaaccgt
    14281 tccttaatat ttgcagatca atagccaatc ccagttcatg ctcactagta ccaggtttag
    14341 caacataatc tttatctgcc gcctcacggt aaagctgtgc ttgttctgcc gcactgcgat
    14401 agccgctagt aataacaaga ttgttcatac cagcagcttg tgccgcctca atcatcaatt
    14461 ctgcctgctg tagtgttttt tcctctagca tagtatcagc cgtctttaac ggcagatgac
    14521 catacgccgg taccagtgta aaagcagact ctccaacggt aatgggcaat gcttcgttaa
    14581 ccagtaataa atggtagtca ttgtcgcact gcggcactgg tactgtagta acagcaatca
    14641 tctcggtctt caccctctgc agcgttgtct gattagtcac ttgctcttgt tctaaaaaaa
    14701 ctagttccac aacgcctaat aacagcaagg ctgccaaact taatttaagc atcttatact
    14761 tttgcatgcg aatgtcatca cctctataat gacattctac ccgagatttt ctaacttcca
    14821 ggaaggaaac tcttaagtat ttcttaattc gcggccgcta gtttcactta cataacaaag
    14881 ccgccaagtt tccctgacgg cctgctaact ccatagtata cactaatttc tttcataatc
    14941 cttgccttca cctagcacgc catgtacttt aggcatccat tcgctaatga gaatgccttg
    15001 cgggcatttt tcttcacaaa tacgacattc cagacagctg ccagcgcgca ttgcttccgt
    15061 cagaaaatgc tgataggtgc cacgggctcc agtaagatcg tcatacataa agccttcatt
    15121 gtaagtttta aaattgcgcg ggatctctat attattgggg caaggcatgc aataagcaca
    15181 accagtacaa ggaatggcgg ttttcgtcag aaagcgctgg cgtgcttcaa ccatcaattg
    15241 ccgttctgct tcactaaaag agttaacccc agacttgtcc gcagcttgga gattctgctg
    15301 aacttgctcc atagttgaca tgccgcttaa gactacagat acttccggtt gattccacaa
    15361 ccactgcaac gcccagtcta ctggggagcg cgtgggatca gcagcatgga gtaaggcagt
    15421 gatgtcttgt ggtggcttaa ccagtttgcc gcccaggagg ggttccatga tcactaccgc
    15481 caggccttgg gccgcagcat attgtaggcc agccctacca gcctggtttt cagtatccat
    15541 ataattatat tggatttgac aaaaatccca ctggtgtcca tcaactattt ctttaaaagc
    15601 tgccaagtcg tcatgaaagg aaaaccccag gtagcgaata cgaccatctt ttttcgccgc
    15661 ttctgctttt tgcagtagat catatttaag gactacttgc tcccaggaat ttttattcag
    15721 accgtgcagc aaataaaaat caatttgctc cgtttgtaag cgtgataatt gttccgtgag
    15781 aaagcgctca aaatcctcag catgcttgta aaaacgaatt ggtgatttgg tagctaacct
    15841 cactttttct cgataaccat cctgtaatgc tttaccaacc actacttcgc tttgaccatg
    15901 atgatagggc catgctgtat caacataatt aacaccctgg tcaatcgcat gacgaatcat
    15961 gtcaattgct tcatactcaa caatgttagc ctgattacca tcagttgttg gcagacgcat
    16021 cgcgccaaaa cccaaagccg aaacctgcca atccaggcga ccaaattttc tgtactgcat
    16081 cctgctgtcc cctttctcta caccttgtac acattatacc atcatttgcc gcgaattaaa
    16141 aaaattctgc cgctcttgaa atgcctttta acaacagaag aaaaaattaa gtccgcactg
    16201 tgcggactta attgctgtcg tgaagctatt tactttgttg aggcagccat ttccttccta
    16261 ttttcatagg catcttttaa agcccccagg atatcaccgt gggcgtcgct taagctcatc
    16321 gtcgctccag atctaatgtc ctcaacttca gatttttgtt catcagttaa tttgtctact
    16381 ttagttttat cttcctcatt atccgtgttg ggatctagcg gacgaccatt acgatcagag
    16441 aacattgtcg taaaagcatc ttcaatttcc gcaacggttt tacccttgaa ccacttttca
    16501 aaggaatcca tttgtttatg ccagtcgtta tcaggattca tatggtagtt atcgccacgt
    16561 tcacgtttag taacccactc ctcaatctct ttggggacac tttcttccgt gttctcagag
    16621 acacctgtca cttttcccgt gtcttcatca gtgatattat aaccttcctt acccggccaa
    16681 ccggagaaat gcggcatgcc ggcgccgtca tagttggggg tagaaacctc aaggatatct
    16741 acttcggcat caacgacttt accctctgca tcgaaaatag cagaggcaaa aacaaagttg
    16801 taactgtaaa caggtacccc ttcactgtca gaacctggac cattgcggaa attactagct
    16861 gtccccactc cttggtaaag ctttacatcc ccagtaggag cgggattttg attgccattg
    16921 ccatttccgt taccactagg ctctttatcc ccacccgcgc aaccaacaag cagtacagtt
    16981 gccataacta gtgtcattag aattgccaaa acctttttca ttactaaatt acctcctttt
    17041 ttgggcttgc tttttttggc ttttttgctt aaagtaactt tctacctttg tcagcaggaa
    17101 gaacctccct taaaaaaagt gttaatgatt tcaagacaaa gtttccccaa ttcggtagat
    17161 aataatacaa aaaggagtgc tgtatcaagt aaggggattt agcttcagct aaatcccctt
    17221 acactttact acttatgctt ttactttttt atttttggca gatttaaatg ctttgcggta
    17281 gagatagcgc accgtcaaca agggactttt ataaagcatg cgcggtccgg cataacgcat
    17341 tacttttttg atggcctcgc gttgttgcgc tgcatagcaa tgcaccgtac acttaccgca
    17401 tactggtttc tgctcgccaa agggacattt atcaatacgt tccttactat aggcaagtaa
    17461 ttcattacag tcggcacaaa gctccgtcgt gctgccatgg ttaccctgac aatagatatc
    17521 aaccatcttc gtaacagttt tttcctcgag tgttgatgaa agtgccacgt tgcttccccc
    17581 ctagtttgtg agtcctttat ttcaggatac acctattata ttttatatcg gcagcattaa
    17641 cccaaacaat gtgggatgtg tgcccgcatc attaggtaga ggcaatacca tgtttgataa
    17701 tagcataaat ttctgtggcc tgctttaaca taccctcata atcagctgat tgataatatt
    17761 ctttgagaca atacaacatg agcgccgtga tcatctcaat aattaattct ggcttaactt
    17821 cacttttaat cacgcctcgc tgttgatccc gtgcaattaa actggtcagt tgcgcaacag
    17881 cttgcggaga aagttcccgc aattttataa taaactcact ttcatctaaa atcatccgca
    17941 taccaatttg gttatattgc ggcatagcgt gggcccaggt caacgctgct tctgtgatgt
    18001 gcaaaagggc agcaaaaaaa tcatcgtcag gctgttgttg tcttgcttga tcgattacag
    18061 ctagtttctc cttgccgatt tctgttagca tatagagata aatatccttt ttgtcgcgaa
    18121 agtactgata aaagctgcca cgtgaaatac ctgcctgctt aataatttga ttgatagaag
    18181 cttctttaaa gctgcacagt gagaattctt gtactgcagc ttgaaatatt ttactttgtt
    18241 tctccttatt aagattaaaa aaagttgttt taggcatgcc gcctcccctc ctgtctttat
    18301 tataacaaaa agaaataaaa tgacaatgtt gtcatattca ataatatagt ttatactaaa
    18361 aaaaaaggag gcgttgccgg tgattacagt aagaaatctc cgctacaaat atcctagaaa
    18421 taaggactat accataaatg gcattgattt tgacattaac acaggcgaaa tctttggttt
    18481 tcttggtccc tccggcgctg gcaaaagtac cacgcaaaag attctcatca aactattacg
    18541 cggctttgag ggtgaaatca attacctgga taaaccactg gccagttttg gcgatgattt
    18601 ttatgaagat gtcggcgtca gtttcgaaat gcctatctcc ttttctaaac tgacagcact
    18661 ggaaaaccta cagttttttc aacaacttta taaaactaaa gttgaagtgg agcctctgct
    18721 gcgacgagta gggctctggg aagatcgtca taaaaaagca ggcgaatatt ctaaagggat
    18781 gaaaatccgc cttaacttcg tgcgcgctct gcttaataag cccacctttc tttgccttga
    18841 cgaacccact aatggtcttg accctgctaa tgcccgcatt atgaaagata tgattctaga
    18901 attcaaacaa gagggcggca cagtcttttt aaccagccat atcatgagtg atgtcgatga
    18961 actatgtgat cgcatcgctt tcattgccga cggtgaacta cgtgagattg atacaccgcg
    19021 taatctgaaa ataaaatatg gtaaacgcag cgtcaacgta gagtatcgtg atcaaggcga
    19081 gctcatcaag cgagagttta gtatggcaga gataaaatcg ccagcgttta ttaatttgct
    19141 gcaaactaag gagattgaga cactgcatag cggtgaaacc acgctagagg agatctttat
    19201 taaagtgacg ggggtggcac ttcgtgacta gactaaaagt gctggtgaaa agcgaatttg
    19261 agcgattaaa taaatataat ctatttaccg cgaattttgt tgtcttgtta ctgtggatgc
    19321 tgctggcatg gtttttggaa ggggaagtgc ttctacaatt catgcccatc atctttctta
    19381 cagacagcgt gatgatgacc atcctgctgg taggagcaac tctcttttac gaaaaaaaag
    19441 agcataccat taactcgatc atgatttctc ccgttacaga aaatgaatac ctattggcta
    19501 aagtgctaat cagtgtaggg aattccctgc ttaccgtgct cttcatctct gctgctgttt
    19561 acttgatgaa aggtgtcaga tatgactacc tccttttaat cccggccgtt gccctagtca
    19621 ctgtcgcgca caccctgctt ggcattcgcc tttcctacag tgccaaggat tttacttctc
    19681 tgttgattaa tttcatggtt tatgtttttc tttttttgct tccttccgtt tttgcagcac
    19741 tggggattat tagcagcaat attgcccaat attttatcat cctgccgccg gaaacttcca
    19801 gcattttaat cagtgctgcc tttgaaaaag ctgatttgtg gaagttgttc tttagttatg
    19861 cttatcttgt gctgctatct ctagtactct ttcaatttgt cgttaaacca aaatttaatg
    19921 aatacctaat gaaagaaacg ggggtgtagg catgcttttg actttaacta aatttgagtt
    19981 gaagaatttg ctacgtgata aaatgacggt ttttatgatc atttatcctc tcctcctggg
    20041 ggggttggga aaatatctca tcgctcacaa ttatgttgat ggtccagctt taggcattac
    20101 tgccatgtta atagcaattc tatccggctt tgcctacggc gccatggcag gcttttcgct
    20161 gcttgacgat cgtgatgatc atgtttttgc ttccttgcaa atcgcgccga tctcgttaaa
    20221 ttcttatatt tggtttaaag tactttttgc ctatctactt gccgtgctgt caaacttctt
    20281 tattgtctgg tttactgctg ccttttctct ttcccctggc aatttattgt taatatctac
    20341 actgtcagca ctgcaaacac caattatcgc ctttattatc aatgcttttg cgcaaaacaa
    20401 agtagaaggt tttgtcacga tgaaggctac cggccttttg ctgttgttac caattgggag
    20461 ctacttcttc cttgatgcca aagaatggct ttttgccatt gctcctggct attgggcagc
    20521 aaaagctgtt cagtatagcc tcctggagcc acaaattgat gctggcttgg tttctatgaa
    20581 cctaaacttt taccaatata tactgctggg gattgcctat aatttgttgc tggtaatcgt
    20641 cgcttttggg atatttagac gaaagaacga catataatta cacagggggt ggattaaatg
    20701 tccggtattt ccatgatctt cttactaatt gctgtggtgt atctgtttgg gactctgttt
    20761 gaattgccac tagtattaga aaatcctaaa tctaaatgga taatagataa aatcggtaaa
    20821 aaacagacca agatcctttt tatcatcatt gctgtaatct tctttatctt ggcctctaaa
    20881 tttaagtaag gcgaactatc agttcgcctc ttttttattt ttgccaaaag tcactcatga
    20941 tcagcttcaa atcactggcg tcacgcacta cttgatagtg ctgccaatgc tgcgggaaaa
    21001 gcttccgata ggtaacttga gtaaaccgtt catcaattaa taatacgata cctttgtcag
    21061 tttcactgcg gatcactctg ccggccgctt ggagaacttt attcatcccg ggataaagat
    21121 aagcatattc atagcccaat tgattctgct gctgatagta attacgaata atattatgtt
    21181 ctgtatttag ctgcggcagc ccgacgccga caatgatggt gccaatcagg cgctcgccct
    21241 tcaaatcaat tccctcggca tagatgccgc ctagtacaca aaagcctacc agcgtctcga
    21301 cattatcttg atcgaaagcc gttaaaaatg cttctcttgc ttgctcgtcc atttcgctgt
    21361 gctggatttg tgtttgtatc tctgcatatt gggcagcaaa gatcctataa acatcatgca
    21421 gatacttata ggaagggaaa taaactaaat aattacctcg tttcctgctg acagtgtgat
    21481 agatcaaatc tgctatctct gctagactat gggcacggtt tttatattta gtactaatgc
    21541 gatctgctaa cactaaacag agctggtctt gcggataggg tgacgacagt gaaagttttt
    21601 tcgcttcttc attaccgccc agtaccttga taaagtagga aaatggtgtt aaggtagcag
    21661 aaaaaagaat ggctgcttta cccttggcca aacgttcctg aagcaaatgt gaagggtcta
    21721 aacaaaacaa tttaacctgc agctcactac catttttacg cagcatggtt acataacact
    21781 gatcatagaa ttcagcaatt tttagatagg ccaaaacatc aaaatagagc tgcaaaaact
    21841 cctcatcgat ctcttgtccc tgtttatctt ttaaccatgc cgcacaaagc agcacaaaat
    21901 ctgttagctg ctttgtcagc tttgctggca gctccttaag cagaaaatgc tcttgctgct
    21961 cacaaagttt acgcaaactt accatcacgc tgtttacttt accaaggctg cgataaagct
    22021 ctttttcctg cttgccaatt tgctttttta actttaaaaa agcagtttta tttaaggtcg
    22081 ccgagaacat ttctctggct ctgtctacca aattatgcgc ttcatcaact aaaaagatgt
    22141 aattaccttt actagtaaaa aatcgtttta ggtaaacctg aggatcaaag aggtaattat
    22201 aatcacaaat aatacagtcg ctccacaacg taagatctaa agctagttca aaagggcaga
    22261 cagtatattt ttgggcgtag acagcaatta tctcccggga gaaatcatca tgttcttgta
    22321 acaattggta taaggcctca ttaacacgat caaagtgtcc tttagcaaag cggcagctat
    22381 cgggattaca gctgcgttct gctaaaaaac aggctttatc tttggccgtc agagttactg
    22441 tctttagctt taatccctgg gcacgcatcg ttgctacagc ctcagctgct acctgtctgg
    22501 tgattgtttt ggctgtcaga taaaaaatct tttctacttc accctcacca agtgctttta
    22561 ctgcagggaa caatgttgag atggtcttgc caatacctgt tggtgcttgc gcaaacagga
    22621 ggcctgagct ggcgatagtt ttatagacgg ccaccgcaaa cttcctttgc ccgtgacgat
    22681 agctgtcaaa tgggaactgc agctttttaa tagcatgctc tctggtaact gcccattggc
    22741 tgccgaagtc tgcccacttt ttaaacatcg ccagcagcgc aaaatagtaa tctgttaaag
    22801 cttctttgct atatattttt tgcagacgct tcacttcatt actttcagca tggacataag
    22861 tgagctggat accaatgctt gtcagctcat gctgcagaca atagatgtag gcataaactt
    22921 tagcttgagc ccagtgcaaa gagttaaaag tttcatcaat ttgtgccaaa gggagcattg
    22981 ttgatttaat ttcatctatc caaaagcttt ctgctctggt aaaaacacca tcagcacgac
    23041 cttctacagt tatggtaaag gttgcacagg ccgtttccgt agagagaaat acttcagact
    23101 gataatcttc ccctgcttcc ttttgcaaac ggcggtggat acgaccccct tcaacagctc
    23161 gctccagacc ggtaaagcga cggtcgatat ccccgctgcg taaaacaaac tcaagcagct
    23221 gtttaactgc tatttttatt ttatatccca ttttgacctc tgcttcctac ttagcatact
    23281 tccattatag cagatatcaa aactaaaact ctcagcgcaa atcaagataa tttgtttatt
    23341 ctggtacatc tctggtataa taggggagaa tctagttaat taacatttaa catccaacct
    23401 agtaaaaaac ttgcgtttgc agtgccgatt gtaatttgat cgtggctgca aaaagtctgc
    23461 taactgcgtt acgatgattc ttttgacagc agaacaaaat aataagggga gattcacatg
    23521 accgaggatt tagaagtgca atccaacttt atctacaata atattactaa agatgtcgct
    23581 gaaggtaaaa atgaaggtaa agtacatact cgtttcccgc cggaacccaa tggttatttg
    23641 catattggac atagcaaagc tattttacta aattaccggc ttgccaaaaa ctttaacggt
    23701 ttgtttaatt tgcgttttga cgacactaac cccgttaagg aagaacaaga atttgtcgat
    23761 tctattctgg aagatctccg ctggttgggg gtggagtggg gagatcgttt attcttcact
    23821 tctgattctt ttgaactaaa gtatgagttt gccagccgtt tgataaaagc tggacgagcc
    23881 tatatctgtg atctttcacc agctgaaatc cgtgaatatc gcggtacgtt aacagaacct
    23941 ggcagggaaa gcccttaccg cagccgttca gttgaggaaa gcttagagct cttcacccgc
    24001 atgcgtcaag gtgaatttgc caacggcgaa cgtgtactgc gggctaaaat tgatatggct
    24061 tctggtaatc ttaacatgcg tgacccggtg atttaccgca tcctgcacgc gagccatcac
    24121 agaactggtg acaagtggtg tatttacccc atgtatgact ttgaccatcc tttcaccgat
    24181 gcgctagaag gtattactca ctctctttgc tccattgagt acacagatca tcgtccgcta
    24241 tatgattgga tcgttgataa tgccttggca ttaatgccag agcatattaa aacccgctcc
    24301 cagcaaactg aatttgcccg tttgaatatg acctatactg tgatgagcaa acgtaaatta
    24361 cgtcgcttgg tagaagaaaa ttatgtcgcc ggttgggatg accctagaat gcccaccatc
    24421 gctggtctgc ggcgacgcgg cgtgactgcg gcagccattg ccgattttca ggaacgtgtg
    24481 ggtgtggcgc gtgccgatag tactgttgat ttagcgttat tagagcactg tatacgggag
    24541 gacttggggc ctaaagctca ccgcatgatg gctgttctgg aacctcttaa agtagttata
    24601 actaattggc cggccgataa aactgaaata ctggagctgg aaaatatgcc gggtgatgaa
    24661 agcgccggcg tacgccaagt ggccttgggg cgtgaaatct atattgaaca agaagatttc
    24721 atggaagaac cgatgaaaaa gtttcatcgt ctcgctcccg ggcgagaagt acgtcttaaa
    24781 ggtgcttaca tcattcgctg tgaagaagtg attaaagatg cagctggtca gatcatggag
    24841 ctgcactgca cctatgaccc acaaactaga agtggtgaag acaccagcgg taaaaaagtg
    24901 aaaggtgtca tccactgggt tgctgttgaa catgccgcta aagttactgt ccggctttac
    24961 gacactttat ttattaagga aaatccagag gatgaaacaa gcggagactt tactcgcaac
    25021 attaaccaag actctttgct tactataaca gaggtaccag tagagccagc ggcagcagaa
    25081 gccgcgcccg gcagccgcat tcaatttatg cgtaaaggct acttttatct ggatcccgaa
    25141 gatgctaaag caggtcaaat agtatataat cgcaccgtag aacttagaga ttcttgggcg
    25201 aaaaagaaaa gataatacca aaagacctcg ttcgttatca gaacgaggtc tttttttgtc
    25261 tgtagtgcta ttttttcatt cccgtataaa tcagaactgc atcccttaaa aatacggcag
    25321 taccaggctg ctcttgatca tagtaagcag taaaccgtgg atcatcaaca tacatttgcg
    25381 ctacacccgc atgtgcttct ttgtgatatt gaccccaggc catcgtgatc cactgtttat
    25441 gcaaagcagc agccttttgt gccaagggac tggccgggtc cccagcaggc atcgcttcag
    25501 ctaggctttt acgtacttct gcctccagct cctgcatcct agttaaatcc tctggcgaca
    25561 tgttcttaaa ggcttggtta gactgcttga cagcctcctc gccatacttt tctcttacct
    25621 ctttgccata cttttgttca ttttcagtaa ttaatttttg cttaaaacca gcaaactttt
    25681 cttgatcaga catggttatc ctcccttctc ttaaagccag tgtcttgcgc acattggcaa
    25741 ttaacgtttc tagctgtgct tgcttatgca ggagttgagc caaatgttcc cgcagcgcag
    25801 agttcgcatc aaaggccggg ttagtgatca tcgctttaat atcttccaaa cagacaccaa
    25861 gttctctata aaatagaatt tgctgcagac gatcaacttc agcactgcca tagatacgat
    25921 accctgatga attgattcta gccggcttga gaatgccaat ttcatcatag taacgcaggg
    25981 tgcgagcact gactcctgcc atcttgccta tttttcgcac tgtgtactcc acttgtttca
    26041 acctcctttc ctttattact ataaaccctt acgttacgtt aaggtcaagc acttttaaag
    26101 atttttcccc tggaaaaata attagctgcg ttgcgagcaa aatctctgcc aaacagtggg
    26161 tattgccttc ttaccagggc aaactaaaga caacgaaatt aaaagaaagg aggccctcca
    26221 tgaataaaga aaaagaaaaa tttatggcca aacctattga gaagcatgac accgcagcct
    26281 gggccgatcg agaagaaatt aaacgaaaat caaaagtaac tatcccgcat gaagtagatg
    26341 tgcgcaatgc taaagaatat gtagactcga atcagaaata atgagcagga aggtctgcaa
    26401 gccttcctgt ttctttatta gcttcgatac agtactcgag aagtatagaa aataaaggca
    26461 aaaaggttgc acttagagaa ctagttgggc gataatataa gcagtaaagc tagaaaggag
    26521 ctgccaagca tgtataaaaa tttagtgccc cgctttttgc gctatctgca aaaggaaaca
    26581 cgttctgatg aaaacagcac aactattcct tccacaccat cacagaaaga atttgcccaa
    26641 actttaaaga ttgaactgca gcaattaggg ttaagcgagg taacttacaa tgaccataac
    26701 ggctatctaa cagctagatt accagcaaac accgcagcgc aagtaccgac aattggcttt
    26761 atcgcccacc tagatacagc agactttaat gccaaaaata tcaatcccca aattcatgag
    26821 cactatcatg gtgaagcaat tgtcttaaac aaagacaaga acattgtttt gtcacctgat
    26881 gaatttccca acttaaagaa ctacttggga caaactctca taactactga tggtacaacg
    26941 ctgctggggg cggatgataa agcaggcatc gccgccatca ttaccgctct agagatatta
    27001 agtaaagacg attccatcaa acacggagaa attcgtgtag cttttggccc agatgaggaa
    27061 attggtatcg gtgcagaccg ttttgatgtc cagagcttta atgccgattt tgcatatact
    27121 gtggatggtg gacccgtggg ggaattagag tatgaatgtt tcaatgctgc ttcggcgcta
    27181 gttactattc aaggcaaaaa tgtccatcca ggcacggcaa aagataagat ggtcaatgct
    27241 cttaaactag cacttgcttt tgatgcctcc ctaccccaag ctgaagtacc cgaaaaaaca
    27301 gcaggtcgag agggttttta tcacttattg cgccttactg gagaggtaga agaagcaaaa
    27361 ttatcctata ttattcgcga tcatgaccgt gagctttttg ccaaacggaa agaaaccatc
    27421 ttaaagattg cccaagagtt gaatgaaaaa tacggaagtg aacgtgtcgt tgtggccatc
    27481 aaagattcct attataacat gggtgagatt attgccaaag atatgactgt ggttaatcta
    27541 gcgcaaaaag ctatggagaa gctggggatt atgcccatta taaaacccat ccgtggaggt
    27601 acagatgggt ctaaaatcac ctttatgggc ttacccacac ctaacatttt tactggtggt
    27661 gaaaactttc atggtcgcta cgaattcatt actgtcgaaa gcatgcaaaa agccacggct
    27721 gtcattgtcg agattgcccg tttaaatact gaaagtaact agctgcttct gttctcctgc
    27781 ggaaagaaaa tagccatagt tgtaccagct gcagaagtat ctatattaat ggtcgcatta
    27841 tagctatcca aaattttaat actaaccggt agtcctaaac ctgtgccgtt ttctttcgtg
    27901 gtgacaaagg gctcgcctag ctttgccatt acttctttgg ggataccact gcctgagtct
    27961 tctatcgcca gcactacttg atctctttcc atatacgtac ggattttcag gatgccacct
    28021 ttaggcatgg cctctaaacc gttgcgcgta aggttaagga ctacctgacg gatatcttta
    28081 gtatcaatgt ctagtggtac aatttcacct gctagtaaag tgacgtcttt atcttccctt
    28141 aaagcatcgg cttgcagcaa gggtaagatt gccagaagga tcttatttaa atcctgccgt
    28201 tggatgtctt tatccttatc acgggccaga gaaaggtatt cagaaataat accattagcc
    28261 cgctctatct catcaaccat caaggctaaa atatcatggt acgcagtaaa ctcatcttta
    28321 ctctgcagca attgaataaa cccttttact gcggtcatgg gatttcttat ttcgtgcgca
    28381 atgctggcag ccatgctggc tactaaatta atccgctcgt atttagcaat attattagta
    28441 caatcatcat cataatcact cttaataaaa gaaaaatggt gactgttaac aatatccaaa
    28501 atttgaggca tgctgcatgc acttagttga tagggacaca aagcaatcat tttcaattta
    28561 ccaattttct tgccgacatt ctcttcatag tctaaaaact ttttccaata cttttttgtt
    28621 aaccaagccg tgttggcaca taatcttaat ccatcatagc ccttagccag tgcttgttca
    28681 gtttttttct gccagcccgc ttctatttta cccacctgga gatcaccata ctttagatac
    28741 cactgatcat gggttaagat ttctagttgt ggctgataaa gatcaaaatt aggcactaaa
    28801 cgcttgagca cactaatcgc tgtactcggg gtaatagaac cagaaacaat ccacatgcag
    28861 tattcattat tttctaaacc tgcccgcaga taggggacaa ctatttccag caaatcatga
    28921 tctgtttgat aaaagccgca aagatgagta ccccagggga tatcttgcag ataagggata
    28981 ccagattttc taagtaagtt taatagtgca gctcttaact gatgagtttc aactgttgtt
    29041 atcgaattac tgctgataaa actttgttgt gacaaagaca cccctcccct ttacatgtaa
    29101 taggaattgg gaattctatt aatattccgt catttaagcc tcctacttga ttcgacgtgt
    29161 agtttgcaaa tcctgcctat taatggtgta gggaccaaat acagaacaag tgcaaggttt
    29221 gcggctatgc agtgagctaa ttgcagtcct tcgctaaaac ggtcaactta acactaactg
    29281 cgtagtaagc taattgctta acctctcagc aagagccgcg gcatagctaa atatgccgcg
    29341 gctcttatct gttttactta atggagatat tctgaaacca ataatctcct tggtttagct
    29401 gctcttcaag tataatctgc aaatcacttt taaatatagg gccgccaata acgactgtat
    29461 ggtactcacc attttcgcct gccggatcaa tgcctgccgc tgcaaactcc gctagcagct
    29521 gttgatccac cacttgaccc agatattttt tgtctaaacg cttaccatct aaagatacta
    29581 ctaaggcttt aaaaccggca gtgataaact catgcagcac ttgctgacgc ggcttttgcc
    29641 aaagcggatg gtaaaccagc atccccgcaa cctgcgatac cctttgcacc cattgtcgat
    29701 gatctgccaa atcaatatct ccatagacac caatctcgat accggctgct ttcatttgct
    29761 ttaatttagt aatgaagtta gcttcataat ctccccacgt agcagcctct gtcgtcaggg
    29821 gaatctcgag caatgctgct tgtttttgca atagtgccag ggacaaacca tgtgaccggg
    29881 aacgttctcc cccttcgttg agcatggtca atagttgttt aggaatcccc ccctcctgta
    29941 tggcataata caatgataga caagaatctt tgccaccact ccaagaacag aaaaacggtc
    30001 tatttttaat tttcattgca aaactcctta agaaagtaaa tttatattat ctgtcactct
    30061 ttaaattctc tttgctgatt gtatattcct ttgtcaattt cggccagaaa aaggctctgc
    30121 ctgctaattt aaaaccactc cgcggagtgg ttttaaatta gctttgatga tcctgttggt
    30181 ctttattaaa caaagcatac atcacaggta cgacaaggag ggtgagcaca gtagcgtatg
    30241 ctaaacctcc tatcgctgtt atcgccaacg gttgtaaggt ttctgccccc atacctacgc
    30301 ctaaggaaag tgtagagagt cccagaattg tcgttaacgc cgtcattaga atggggcgca
    30361 gccgggtttt acctgctgta attaaagctt cctttactga cagacccttt cccttgattt
    30421 gattggtata gtcaacaaaa acaataccat tgttaacaac aacaccagag agcaccaaaa
    30481 acccgagcat cgcaattaag ctaagctcat agccagtaat tgctaaagcc agcaaacctc
    30541 ctgtaaaagc tagggggata gtaaacatca caataaaggg tgataacagt gattgaaact
    30601 gtgctaccat aattaaatag atgaaaacaa tcgctaaaag caacatataa ataagatcaa
    30661 caaaagaatt attaattaac tctgtttcgc cagcaagttc cacgcgatag ccgcttggta
    30721 gttgatagtc ctgcagcttt tcctcaagtt cacggctgac taggccaata ttatggtcag
    30781 catcgatact tgccgttact gaaatatatc tttcttgggc atccctggtg atagaggaaa
    30841 gcccttccgc ctcattgata gcagcaatat ctgccagctt aacttctgta gactcgccat
    30901 tttggctgcc tgcaaccact aaatctgcta cattgtcacg tgtcagcgtt tgacttttcc
    30961 catcaatgat tatcacagga tagtcttgat tagcaaccga aagcgtagtg gcatttttat
    31021 ctgctagcag tgaattaaca ctggcaaaaa cttgtgccac agtgagacct ttttccgctg
    31081 ctttttcttt attgacagcg attcttaatt cagtagattt ttgttccatc ccgtctgaca
    31141 cctccaccgt accggctgtt gcctgcataa tttgcgtaat ctcagcggcg atctcacgta
    31201 aggtatcaag ttctctacct ttaatgataa cttcaagtcc cgctcctccc agagcagaga
    31261 tatccatatt cgaactagta acagtaatgc tgcactccag atcagcagtt aagcttctta
    31321 tctcagccgc gatttccgta ttgcttctgg ccttatcttc cttcagtacc agatatagag
    31381 acacagaatt tccactgccg ccactgccca gaccacccat accacccatc atactgtttt
    31441 ggaaagcacc tatcgtctct atttctgcca gctcatcgat tctctcaatc acagtttcgg
    31501 acatcgctaa agcctcggcg aaagatgcct cggggggcat ggtcaccgct actgacatct
    31561 gtggcgcttc catctccgga ataaaagatg tgcccatgga caaagctaaa aacccactta
    31621 ggcccaacaa agcaacgaca aagactaaaa ctattgcttt atgctgcaag gaccaacgca
    31681 gtgtgcgctc gtacacctta agcaggccat caaagaaatg atgttctttc tcttgcgtct
    31741 gattaagcat tattgaagcc agggtgggca ctaaagttat cgctacgatt aaactggcaa
    31801 atagggagta agcaatcgtc agccccatat cggcaaaaat ttgacgtgtg atgccttggg
    31861 taaacacaat cggcaaaaac acacaggcgg tcgtaaaggt cgaggctgcg attgctccca
    31921 tcacttcttt tgcaccctca atagcagcct gaataacagg cacgccctcg tttcttaggc
    31981 gatatatatt ttcaataacc acgattgaat tatcaaccaa catcccaatc cccagagcta
    32041 ggccagctaa agaaataata ttcatagtca aaccactaaa atacatcatg gcaatagcaa
    32101 agacaacact gatggggata gacaaagcaa taatcagcgt aggttttaga tcccttaaaa
    32161 atagccataa aattaagacg gctaacaaac cgccataaat tagattatcg agcactgaat
    32221 caataacgat gccaatataa actccttgat ccattaaagc tgttaagtta agcttttcgt
    32281 tgctctctag caactctgca aacttctttt ctatactatc agaaacggct gccgtagaaa
    32341 aattactttg cttttgaaag gttaaaatca cagcattatt accattaact ttcgcatagg
    32401 tgtcgtcagc attatcttta actgcgattt cggccacatc gtccaaacga attttttcta
    32461 tgccactgat accggtatca aacaagacta attttgtcaa ttcatctact tctgacagct
    32521 tatcgccgac cttaactaaa tagtcagtgc cggattccgt gaggtagccg gcaggcatcg
    32581 caaagttctg tgccgctaag acaccagcga tcatcttcgg tgttaaggca ccctctaaag
    32641 aagcattttt gaaggcctct tcttttgccc tttcgaattc agttattttt tggttaagtg
    32701 tgatttcacc acttaaaagt tctgtttttg ctttctccat ttctgcagct agcaattgcc
    32761 tgccgctctg cagctgtttt tcctgctcgg ccaaggactg cttctgttct gtcaaaagtg
    32821 tcttttgctc ctgcagcgac gctgtctgtg cagtaataac tgctaagtca ttattcgttt
    32881 ccgctttctt tgccgctact gcagttaaac tctgtgcaat aaactctagc tgtgtttgtt
    32941 ctgcctctgt tagttcttct tgtttcagca gcgcttcctt ttgcgctatc agctgctctt
    33001 cactataatt aatgacatca agcacactct gcagggcttt ctttgtctgt tccagctgcg
    33061 gctcaccagc aatcatttgc acctctgcct gctcaatttg tttaagcgct acagcaattg
    33121 cttgggcacc tgcagttatt ttggcgcttt gtttttcatt ttctgccgct agttgagctt
    33181 tgccgctctg aatttttttt cttgctgcta gtaactgccg ctctgcttgt gctagttcag
    33241 catcaacatt agccaggatt ttttgattta gttctgtgat tttctcggag ctaatcacaa
    33301 cttcaatctt ttcttccaat aaacccacac cagaaactga ggccacaccg tttacacttt
    33361 ctagctccgg gagaatttct ccgcgcacta tttgtgaaac ttcggcagta tccttgcctt
    33421 ctatatctac agctgccacc atgatcggca gcatctcagg gttcaagcgc ataatcattg
    33481 acgatcctac agagtcactc caagcagctt taattaaatc aagattaccg tttatctcaa
    33541 tagttgctga gtctaaattc acatcattat tgaactctaa aataacgatt gatgaatttt
    33601 ctctggaaac tgaacttata tttttgatgt tgttggtagt tgctaccact tgttcaatgg
    33661 gtcttgtgac caccatctcc acttcctccg gacttgcacc gggataggaa gtcatcacca
    33721 gcaaataggg taaatcaatt ttaggcagga gatctgtttg cagattgata aaggaaacag
    33781 ctcctaaaat aagcaccaaa attacagcaa caaagacagt ataccgtttt tctacactaa
    33841 acttgggcaa catgtaattt ccccctaagc tgttacgcta ataaaatgac taagcggtca
    33901 gatacttcat agtgtagttt aaatgtgcag gtattgtcaa tatgccgaaa gctttttcaa
    33961 ttgcagccaa gaattacgat tttatccgca caaccatcga ctatctattc cggcgtctca
    34021 ctagatgtag ttaaaaaagg ccactcgctt agagtaagcc tcttttactt acttaagccg
    34081 catctctttg atcgatctct ttttgatatt ctgctaattt ttctcttgtt agtctatagc
    34141 caaaggtaat gatcagcgcc gcggccagag caaaaatacc aggaaccaat aagaagacat
    34201 tagttacagc tgttttcagc tctagtgtag cagttgctgg atcagcaccg acgataaaac
    34261 cagctaaccc taaaattagc gggattaagg tacctcgcga aatcacagca gtttttatgg
    34321 aaatgttcat cagacccatc acaaagggag aagcattctc cccggtcttc cattcaccat
    34381 aaacagcagc atctgtatat agggcaacca tcaccgcagt caatacacct aagaagaatc
    34441 tggcaaccaa gacgacaata aagaagcttg taacattgaa ggcgagcagc ctagtaacaa
    34501 ataaggaaac ggctagacca aacagactga cgatcgcagc gcttctggtt gaaagcactc
    34561 ttgccatgtt accgctaaag taagcaccta tcacttgcgc aatagcaccg gtaagcagat
    34621 aaactggcaa taacgcctgg ttttgcgcca catagataaa atagtaggcg gcagcagcac
    34681 tcataataaa gttgaccata taacggaaaa agtcacccac tactaacatc atcaaaggcg
    34741 ggttttgtat gaggctttta accataatgc ccaagcttac tttttgtgcc ccaggagcaa
    34801 catccgcttc ttcacctgtt tcctcataac catcggttat cttaaagact atccaatagc
    34861 caagcagcat ggtaaaagcc atcacccctg cgagcagtga gtaacccata acttcattac
    34921 cggttaaatt acccagcatc acggccaagg gcaaaccaat ataggagaag aagaccccag
    34981 ctaaactcgt ccaagtaccc cttctagagg ctaacagccc tctttcttca gggttatttg
    35041 ccaatacatt aatcagagca acattaccga cccaggcaat attccaaata atatgactca
    35101 agataaagcc cgcacaaacg ataatggccg caattggctc ggggccaatt ctggtgtact
    35161 ggaaagcata gaggacgaca actgctggcg gtgctatcaa cagccaggaa cggttacgtc
    35221 cccactttaa agctttggtg ccgctaataa ttgctccata aaatggagaa aaaacagcat
    35281 ccacaatact tgtcaccgag ccaataagtg cgacgacagc cagtgaatat ttggcaacat
    35341 cagttaagaa atatacgaag taaaacaatt ctacactggt cattaaggaa aaaccaaggt
    35401 cacctatccc gtagtaggtt tttatagctt tgcttaacgg ttttttttgc atcagctaaa
    35461 ctctcctttt gtcttcagat tatggtgtaa aaaccagtac caaaaggcat atgctttagc
    35521 tgtagtttct cccaatgtaa agttaatatt tcgtcttttt ttggctaagg gccgcacaaa
    35581 aaaaggagca ggccctgatc tgaatcccca aggcttgccc ctttttgttg atttattgtt
    35641 tgctcattat tctatgtggt actttcctta ctctgagttg agtttgtaat ctttttgata
    35701 atttcattga caatcaacgg cagcaaggaa aaagcaaaca ctaaacccca atcacgaaag
    35761 cttaaattat gcactttaaa agcattggcc aagaaaggca ctgaaatcac accgaattgt
    35821 aggataaaac caacaatgat ggcgccaatt aaatagatat tagaaaaaat gccgatttgg
    35881 aaaattgact tgctcgaact gcgcacagtt aatgaataga acagctgcga ggcagccaga
    35941 acaacaaaag acatagttct agcatacgta aggaccgtct cagggatatc tgtggaaccg
    36001 agactatagc cgtgttcact taggccaaag tagaaggcca gcagcgtcaa tgtgccgatc
    36061 agtgtaccgc ctataataga ccgggttccc gccccgttag caaagaaact ttctttagga
    36121 ttacgaggct gttttttcat tacatcttta tctccaggat ccacaccaag ggcaatagcg
    36181 ggcagggaat cagtgattaa attgatccac aagatttgcg taggcagcag cggcactggc
    36241 caaaagaaga gaatcgaggc aaatacggct attacttcac ctaaattaca ggaaagaagg
    36301 aagatcacag acttcttaat attattgtaa atgtttcggc cttcttcaat ggcgcgtaca
    36361 atggtggtaa agttatcatc cgtgaggatc atatcactgg cacccttaga aacatctgtg
    36421 ccggtaatac ccatggccac gccaatgtcc gcatatttta atgatggtgc atcgttaact
    36481 ccatcacctg tcatggaaac aatattaccg tgcgctttat aggccctcac aatttttact
    36541 ttatgttcgg gtgaaactct ggcaaagact cgataattac taatttgctc ggagaattcc
    36601 tcatcgctca tctcatcgat ttctgcacct aaaagactct gctcgatacc atcagcaatg
    36661 ccaagttctt tggcaatggc tacggcagtg ttgcgatggt ctcccgtgat catgattggt
    36721 gtgatccctg ctaattttgc ttccctgatt gaatccttaa cttctagtcg cggcggatca
    36781 atcatgccta ccaaaccagt taaggtcagc tcttcttcca tctcctcagg ggtaatcagc
    36841 accgtagtat ctttataggc agcacctagg acacgcaggg cgttattaga catctcgtca
    36901 gctatcttca ggtatttttc cttcatggaa tctgttaacg gcacaacttt cccatctact
    36961 agggctgttg tcgcaagatt aatgatatta tcgatagctc ctttagtatg tactctgaaa
    37021 ccatcgcctt ccttattaag cgtagacatt agtttacgat tagaatcaaa aggtttttct
    37081 gctaccctct gatgttgact atttaattta actttagcaa gattgaagcg ctcacctaac
    37141 accactaaag caatttctgt ggggtcgcct gtactctcgc cattttcata ggtggcatcc
    37201 gaacataaga caaaggtctt cataagatta attttatcaa cattagttat ggtaaaatcg
    37261 ctgccggttt taggaacgtc tgccaagtta ttggctgtat aagttttgac aacagtcatt
    37321 ttattttggg ttaaagtgcc ggttttatct gaacaaataa tatttaccga acctaaagtt
    37381 tctacagcag gcagtttttt cacgatagca ttgatcttgg acatttttgt tacacccaga
    37441 gctaaaacaa tcgctacaat agccgccagg ccttctggaa tagcagctac cgccaaacta
    37501 atggccgtga gaaacatctc aaatagatct cttttctgga ataaggcaat aacaaagatt
    37561 aaggcacaaa taccaatagc aataaagcct aatattttgc ccagttcttc tagcctcttt
    37621 tgtaaaggag tcagctcttc atggtcttcg tcaagaattt tagcaatttt acctacttct
    37681 gtctccatgc ccgtagcgat agccacgcct tcacccctgc cataggtagc aagggtagac
    37741 ataaaagaca tattagattt gtcaccaatc ggcgttttag gattcacatg cagcttttct
    37801 gcgttcttat ccgcaggtac agattcaccg gtgagggcag attcttcaat ctgtagatta
    37861 gcgctgtcaa tgagccgtaa atctgccggg atatatcttc cggcatcgat gatcacaata
    37921 tcgccgggta caacttgctc ggaatcgatc tcttttactt cgccatctct cctcacaatt
    37981 gctttcggtg tcgtcatctt ttgcagggct tcaatcgcct tttctgcttt atattcctgc
    38041 accacaccaa tcacagcatt taaaattaca accaagagga taatgacagc atcaacatat
    38101 tcttgaatca ctaaggtaat cgctgttgcc cctaacaaaa cataaatcag catatcctgc
    38161 agttgggcaa aaaatagtac gattaaactt tttttcggtt tactcttaag tttattttcg
    38221 ccatacttgt ttagcctttc ttgagcttct tcactcgaaa ggcctgcggc tggatccaca
    38281 gcaaattctt ttagcacttc ttcttgcgac ttggcaaacc acatagccta aacaccctcc
    38341 atttttgttt tcttattttt tctttgtctg catctattta tgccctcagc ctattaacac
    38401 aagcactgcc tagtaaacaa aaactctgca cctagacttg ccacggcaag acagacgcag
    38461 agtctcacga tttaaaacaa taaagccggg ctttattaag agccgtaatg acgactttat
    38521 gctgctgttg agcagacgtt actccctctg gtaattaatt caattaagtg aagtttatca
    38581 tgcctgtttg tttacgtcaa tagcttttta gaagcagtaa aagcagatga agtctatttg
    38641 ccgaatttta atgcagcgtt attttatctt aagattacca cagtaacacc ggcattattt
    38701 gccactttga agttaaagtt ctctaatgct ttacacatgc caatcatttc tcttttgcgg
    38761 tctatccact gctcaccgcc agcataggtt ctgactatac ccttcagact atgttcacta
    38821 agtacttttg ctaccgccgg tttaattttg cctcctaccc ctgtagaccc gtaaccatga
    38881 ataagaataa cagctttata gccttgacgc ttagctgtag ttagggcatt tttcattctt
    38941 tgtatcgccg cctctaccgt cggcaatcct tcctctaaat tgacttgagc aacctttttg
    39001 cgcataataa cctccttcgc gactactaat aatctgctct gcaaacgcat atttatctaa
    39061 aagttatact ttacatcatt atttgtcaat aactagagaa gtcaataaaa tccgcagtaa
    39121 gcagatagtg atactattgt ctaaacttcc taaaatatac ttttcctatt gataaacttt
    39181 tcacaccgtg ttacattaaa atttgtcagt gtttttacct tgcctaaggg ttaacctgac
    39241 atagaagaat gaaaaagggg agaactaaga tgaaaaaagc ttcatgcatt ttgcttgtcc
    39301 tggtttcgtt gtttgtgttg gttggttgtt ccggcgggaa gacacctgct aatgccgatc
    39361 ccattaaaat gggaagagtc gagcatgctg cccatgggac aaaatgcttt gcagttgccg
    39421 tagtagctgt acaaggtgac accattgttg gtgcttcact ggatgaatat caatttatga
    39481 gcactgatgt tgctaccggt gttcccaatg ctgatgcgga cttcgctaaa aactttgctg
    39541 atcccgcagt tgttttagct tcaaaacggg caaacgccga ctactacagt gagcatatga
    39601 cggaagcagc tggcgcaaca aaacgtcttg atgaacagta tgacaccatt gaagcatacg
    39661 tagtgggtaa aactgttgcg gaattagaaa gcaccctgtc cgaaaacgac ggcgctggca
    39721 tgattgatgc agtaagttct gttaccttag tagatactaa aggctatgta gaagcacttg
    39781 ttgctgctgc taaagctgca aaataaattc tccatgatca cgttacaatt ttaatagtaa
    39841 gaaaatcgcg cctaggacat aggcgcgatt tttatgtagt tacattctgg tttatttact
    39901 gatttcccaa taacaccttg tgggggaaac gattttttcc tcttttttaa gttttttcat
    39961 tactttgtcc acttcttttc tgtctacacc cgtagcggtc gcaatttcgc cggcacttgc
    40021 aggcttgtcc gctttgttga agaaatcaag tactactgca taactatcca cagcgatcct
    40081 tcctttcctt catttgatag tttatcttat tatagcacgc tgtaaaaaac cttacaaatc
    40141 tctttttacc attcttacct gcaagccagc aatattctct gacaggcttc tagactagct
    40201 tcttttctag gtaatccgct aagggctcga gccaatcacc ggcaaaaagt tcgatttgac
    40261 ctctatcaac ggcataagca agtttaggat ctataggtag ctttgccagt aaatcaatat
    40321 tgtgctgctg tgcagtttct tccaagtggc tatcaccaaa tattttatga ttttctttgc
    40381 actcaggaca ttgaaaatag gacatgttct cgactaatcc taaaatagga atatgcatca
    40441 tttctgccat tttagccgcc ttagaaacga tcatggacac aagttcctga ggtgacgtta
    40501 caatgataat gccatcgata ggaattgatt ggaagacggt caggggaaca tctcctgttc
    40561 ctggcggcat atcaataaac ataaaatcaa catcctgcca gatcacatcg gtccaaaact
    40621 gctttacggc acccgcgata atgggccccc gccaaacgac aggatcggta tcattaggca
    40681 atagtaagtt aatagaaata atatcaatac ctgttttgct ttgtacagga taaatgcctt
    40741 cttcactgcc ggtcgctttc tttttcaggc caaaagcttt ggggatggag ggaccggtaa
    40801 catctgcatc taagatcgca ctatgataac cttttctatt catcattact gccaaaaggg
    40861 aggtaacaag tgatttgccc acaccacctt ttccgctcag tacaccaatc actttcttaa
    40921 ttttacttaa cttatgtggt ttagcggaaa aatcaggctg agcctctttt ctttctgcac
    40981 aatcctcacc acaactgctg caattctcgt tacaactttc actcatgctg actatctcct
    41041 tcacaaatat tatttaccat gttgaccaaa cctgcgtcga cgacagcgac cacagccacg
    41101 gccgcgttca ccttcatcat atagtttgta atcgccacct tcaatcttta gtatagtgcc
    41161 gctaacgagc gcctcagcaa tttttttgcg cgcctcattg tagatacgct gcacagttgt
    41221 gcgcgccaca ttcattcttg tagcacattc ttcttgcttt aacccctcta aatcaattaa
    41281 acgaacactc tcatattcct ctacagtcat gattattact tcctgatctg tagaaaccga
    41341 gtttaaaggt ccaaaaagat tgctttcagg caggcagcat acttttcgcc attttcgcgg
    41401 tcttgccatc atctattcac cccagttaac ttttctacaa ttatacagtt gagggtaagt
    41461 aacaggttat ataacctgtt acttatcctt tggctctagt tctgataatt ggctgctgat
    41521 catctttaat cttctttcta gtatctcttt tttttctgct aataactctt ttctggcagg
    41581 gaagtcagct ggctctgcga taaaacctct gccataacct cgcccgcaac ctctagctcc
    41641 gcgtccatag ccgccaacaa agttagtatt agcctttaca caaggtccaa aacctccccc
    41701 tgtgaaagaa cctgctccca tcggtccagt gccgtccatt ctaggcatct gattacaccc
    41761 cctttcatta ttgacatatg cccctttcaa tttctattat actacgtttt gagcatatgt
    41821 caataactta agcaaaaaaa cccggctaag cgcttaaaca cttaggccgg gttatttata
    41881 cttttaagtt taaccacact tggagtaacc acagtgaatg cagacgacac agccgctttc
    41941 gtggttaatg ggcatcccac aatctggaca ggccagatcg atcgccacac cggtacttgc
    42001 ttttttcttc atcccgttca ccggaatttt tttcacatat tcaccagatt tagcggcgac
    42061 atcggataac ttctgcaatg acttggacaa accattgcca ttagctttgg ctgtagaaga
    42121 ctgaattgct tgtccatttt cagtgccgcc cacatccata tacttcttaa taacccgggc
    42181 aatggcatcc ggacaagagg taacattaac cccttcacgt cgaatacaag cagcacaacg
    42241 aatgcctttg atttggttaa gaatggcgtt gacagaaatg ccagaacgta aagcaatgga
    42301 aattaatctt gcggtggctt ccgattggga agcacagcca cccgcacgac cagtattggt
    42361 aaatatttcg cagatgcctt gttcatccgc attaacactg acatatagat taccacagcc
    42421 gatttttatt ttctctgtta cacccatggt aacttccggc cgaggacggg gggtgattga
    42481 gttgggcgct tgtacttggg cagcaggcgc tgttgctgct ggttgttcct ttgcagtacc
    42541 aaatgagagt acttgttcat cccgactacc atcccgataa actgttaatc ccttacaatt
    42601 tagttcataa gccaacagaa acgccctggc aatatcttcc ctggtagccg cattagcaaa
    42661 gttgatagtt ttggatacgg cattatcggt atatttttgg aaggcagcct gaatctgaat
    42721 atgccattct gaaggaatct ctgaagctgt aagaaaagct tcctgtaccc atgctggtac
    42781 ttcgtctaat ccttgcaccg agccagtctc tgcgatttta gccattagct ggggagaggc
    42841 aaagccatac tcttgcgcat aaccttcaaa taatggatta acttccacaa aagagttgcc
    42901 atctaaaaca tttttggtgt aagctacagc aaaaataggt tctactccac tagaggcgcc
    42961 acaaatcata gaaatggtgc ctgtcggagc aatagtagtg atggtggcat tgcgcatttt
    43021 tcgctcacca tcatagatag aaccttcata attagggaag gtaccccgct cattcgccag
    43081 acgttcagat tctaggtatc cctgtaattg aataaacttc atgatctttt ctgcggcctc
    43141 tactgcttct gctgaagcat aggggatctg cagctgggct aataaatcag caaaacccat
    43201 cacaccaagg ccaattttgc gattaccttt gaccactttt tcgatatttt ccaaagggta
    43261 ctgattggca tcaatcacat tatcaagaaa acgtactgct agtctggtaa tttcagctaa
    43321 atgctcccag tcaattgccg ctccctcttc ttgctctttg accatacctt ttaaattaat
    43381 agaaccaagg ttacaggctt cgttgggtaa gagcggttgt tcaccacacg ggttggtagc
    43441 ttcgatatca cctagtttag gtgtagggtt atctttgttc aagcgctcta aaaagactac
    43501 gcccggctca ccattacgcc aagcatggtc aacaatttta ctaaagactt cagcggctga
    43561 tagttgggcc acaacttctt ttgtatgcgg atccactaaa tcatagtttt gctgctcttt
    43621 taatgcttcc ataaattctt ccgtaatacc aacagaaata ttaaagttag taatctcctg
    43681 attatcttct ttacattgaa taaattccat tatatcaggg tgatctaccc gtaaaatacc
    43741 catattagca ccacggcgag taccgccctg cttaacagcc tctgtggcag cgttaaatac
    43801 tttcataaag ctgacaggac cggaggcgac accaccagtg gaacgtactg aactattttt
    43861 gggacgtagg cgggaaaaac taaagcccgt acctccgcct gatttatgaa taatggccgc
    43921 aaacttaatg gcgtcgaaaa tagcttccat actatcttct accggcagca caaagcatgc
    43981 acttaactgg cctagatctc tacccgcatt cattaaagtc ggtgaattag gcataaattc
    44041 tagattagcc atcatctcgt aaaattgttt agttaatgtc ttaatctctt gagatgtctt
    44101 tgcatattgc tgctcgatct gagcaacaac ggcagcaacc cgatataaca tatcctccgg
    44161 cgtttctaca atctgttcag cgtctttctg caaataacgt ttttctaaaa caaccttagc
    44221 agttgaagtt aactgcattc taggccaaga ttctggcaca ttcctctgag cattcaagcc
    44281 aatccccaac tttctttatg tattcaatcc acatcctgca gcaacaaaac acaacatctt
    44341 gtgtgtctac attttaccaa ctctatatat agtagtcaat agcattttca ttcgacaaaa
    44401 ctatttacca attcatccag aaactatccg ttagttagat taagaaaata aaagcagact
    44461 caaggccatc acggccatac cggaaacaag acccagaata gctatatggt gttcaccgta
    44521 cttttctgcg gtcggcagaa gctcatcaag tgaaatatag accatgatcc cggccacccc
    44581 agcaaaagta atcccaaaga cagcatcact taagaaattc aacaagagaa aatatccgac
    44641 cactgcgcct atcggttcgg caaacccaga tagtaaagag tataaaactg cctttttacg
    44701 atcacctgtg gcaaaataga cgggacatga aatcgcgatt ccttctggaa tattatgaat
    44761 ggcaatggcg atagcgatac ttaatcccag cttggggtct tgtatcgcac taacaaaagt
    44821 tgctaacccc tcgggaaagt tatgtatcgc caccgccaga gcagaaaata aaccgatcct
    44881 taataggttg gcatcattag ccgagtcatc cacgtcctga atctcatggg ggttatccgc
    44941 gctaggcaca agcaaatcaa tgatggcaat taaagcaatg ccggcaaaaa atgccaatgt
    45001 cgtcacccaa taaccttttt tagcgccata gataagcgtt aaagatgccc tagcctcagg
    45061 gaatatttca atcatcgaaa cataaatcat caccccagca gatacgccca aagctgccga
    45121 taaaaacttt ttatttgttt gtgtagtata acaggcaaat aaacttccca cgactgtcga
    45181 taaacctgca aacagtgtaa ttccaaaagc aaacaacaaa ttattatttt ctggcattat
    45241 tgttcctccc taacttgacc tctgtctcta aatttgcagc atcagtagca tagacatatg
    45301 atctaattat attagtttgc ctaaaggagc tcaatcatta ttttttgttt gtggggtcag
    45361 gcttgaatta ttcacttatc tccagccatt tctgcattac ttctcttaca taagctgtaa
    45421 tctccacttc gccactttta attttagaaa tcattgatgg cgacaagttt agcttttgtg
    45481 ccaatgtcgc atttttcacc aggcactgct tctcacttaa taacactact gctttgcgga
    45541 gatcagatat cttttgctgc ctagtcttct taataagttg ctcgtactta atctgttcaa
    45601 gccgacaaac ttccttaatt aattcgtcta tgcctatttc actaccacta gtagcctttt
    45661 tacttttttc ttttaatact ttaggtttta cttcatacat actttccgct gattgtatat
    45721 cgggctcctg gttcatgaaa tacatatatt ctttcagagc ttgctttgca ttgtcagaaa
    45781 acaactgcaa gataaaagag gtgtcgacta ggtcaagact acctttcccc agataattta
    45841 gatggctgct ccacttgtac tctaccccac caggcaaatg agttttgacc gggttgttat
    45901 gaatatatct aatcaactgc aataaatatt tttctttatt acataattga gccttatatc
    45961 tttgttgaaa tacatgtccg gttctctcat atttgtagtt aaaccattga gtataaactt
    46021 gttgtattct ttgcataatt tttgctagtg tgacgtcctg tacttctatt aataagtggg
    46081 catgattatc cataatgcaa aatgagtata ttgaaaagcc caacgcattt ttgtattggg
    46141 ctagcaggtc tagatatttt cttttgtttt tatcattgag gaaaatattt tctccgttat
    46201 taccgcgaac cataacatga tataaagctc cgtaataatg tatacgtggt ttccgcacca
    46261 tacacatcac ctggacaatt ataccatttc agaccactct tttcaagctc tgccgcctgc
    46321 tatgtttaac ttatgcctgt ctcttcgtca ttcaggcccc cccctacaac tctagaaata
    46381 attgtttttg taagatataa tttaggcaga taaatataga agggagatga accggtgaaa
    46441 gtcattatgt atagctcaga tatatgccca gattgtgttg atgcaaaaaa acaattagcg
    46501 gctttgccta aactcgagtt ggattaccgg agcattactg aaagcaccac aagattaaaa
    46561 gaatttttat cgtttcgtga tcatgaggag atgtttgtct caattaaaga gcatggcaaa
    46621 ataggcatcc ccttttttat tttggcagac gggacaaaga cttttgatgt aggtgatttt
    46681 gtccagatcg ataggccaac gtcgtctgcc agttcttgtt ctttagatgg tgagaagcag
    46741 tgttaacccc aaaaggtgct aaaatttaat tcgtctgtca gggcggtaag tgaataattc
    46801 aagcctgacc ccacaagttt gtcacagatt tgtaatattt tattgcatta aaggacaagt
    46861 cgggctataa tggtaatatt aaatattcta cat
//
LOCUS       DASRMS010000106         3976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_363977, whole genome shotgun sequence.
ACCESSION   DASRMS010000106 DASRMS010000000
VERSION     DASRMS010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3976
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_363977"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="VFC74_05025"
     CDS             <1..880
                     /locus_tag="VFC74_05025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006566260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HZK24734.1"
                     /translation="LNTFFLKDKSLIDYVQKVVGLAAIGKVYVEALIIAYGEGRNGKS
                     TFWNAVSRTLGSYSGNISADILTVGCRRNVKPELAEAKGKRLLLAAEMEEGMRLSTSN
                     VKQLCSTDEIYAEKKYKDPFSYIPSHTLVLYTNHLPKVGALDTGTWRRLIVIPFAARI
                     EGHDDIKNYGDYLFAQAGGAILSWIIEGAKKVIKDDYQISLPEKVRDAVSAYKENNDW
                     LAHFLEECCEIDRTFTEKSGELYSEYRAFCLRTGEYARSTTDFYTALDIAGFIRHRKK
                     TGVIVRGLKLKSDFLD"
     gene            1027..1323
                     /locus_tag="VFC74_05030"
     CDS             1027..1323
                     /locus_tag="VFC74_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014119958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HZK24735.1"
                     /translation="MGEKHIEQKLVKAVKAAGGMAPKFISPGLAGMPDRLVLLPKGKM
                     AFVEVKSPGMKPRPLQIKRHRMLRHLGFKVYVLDDATEIKKVLAEVMSDGVHTT"
     gene            1304..2663
                     /locus_tag="VFC74_05035"
                     /pseudo
     CDS             1304..2663
                     /locus_tag="VFC74_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006524189.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
     gene            2666..2878
                     /locus_tag="VFC74_05040"
     CDS             2666..2878
                     /locus_tag="VFC74_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24736.1"
                     /translation="MEDCFAYRGNSCTALKVKKCEGCSFYKTKKQYELDQLKAMERIL
                     SLDAKRREYIINTYYNGKLEVGTFEG"
     gene            2868..3308
                     /locus_tag="VFC74_05045"
     CDS             2868..3308
                     /locus_tag="VFC74_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019278795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1492 domain-containing protein"
                     /protein_id="HZK24737.1"
                     /translation="MRVKEYLSQALWLDKRINSKLEQLEVLRALATKVSANLREEKVS
                     GGNNTKSHLENTVVKIVDLEKEINEDIDRLVGIKTEIMNTIGQVDDPISQLLLEMRYV
                     NGKSWDAVARELRFDRSWISRLHGNALKEIEGRVKYASKSNKKQ"
     gene            3512..3805
                     /locus_tag="VFC74_05050"
     CDS             3512..3805
                     /locus_tag="VFC74_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24738.1"
                     /translation="MKCVSCRFAVIDEAASDRDWAAYECGNPKSEYHKSLINIDEEGK
                     KHKRISWSGCEHGERKVKANAKETLKALSLSRLSGTDGGAVLSQTHERIQPAV"
BASE COUNT         1219 a          811 c         1028 g          918 t
ORIGIN      
        1 cctcaacacc tttttcctaa aggataaatc gttaatcgat tatgtgcaga aagtagtggg
       61 tctagctgcc attggcaagg tttacgtgga ggctttaatt atcgcctatg gcgaaggcag
      121 aaacggtaaa tctacctttt ggaacgccgt ctcaagaacc ctaggtagct acagcggtaa
      181 tatctcggcc gatattttga ccgtaggctg ccgcaggaat gtgaagccgg aactggccga
      241 agccaagggg aaaagactgc ttttggcggc ggagatggaa gaaggcatgc gacttagcac
      301 ctccaatgtt aagcagcttt gttccaccga cgagatttat gcggagaaaa aatataagga
      361 tcctttcagc tatatcccca gccacacttt agttttatat accaaccatc tgccaaaggt
      421 tggagccctt gatacaggta cctggaggcg actgatcgtc attcccttcg ccgccagaat
      481 tgagggccat gatgacatta aaaactatgg cgattacctt tttgcccagg ccggtggggc
      541 cattcttagc tggataatcg agggtgcaaa aaaggtgatc aaggacgact accagataag
      601 tctccctgaa aaagtgcggg atgcagtttc agcctataaa gaaaacaacg attggctggc
      661 ccatttcctt gaggaatgct gtgagattga tagaaccttt acggaaaagt cgggtgagct
      721 atatagcgag taccgggcct tttgtctaag gacaggggaa tacgccagga gcacaactga
      781 tttttataca gcccttgata tagcggggtt tataagacac agaaaaaaga ctggagtgat
      841 cgtgaggggc ttaaagctaa aatcggattt cctggattag gtcattttct ttttgggtgt
      901 agataggagt aggtcatatt ataaagtttt cttaagggct taaaaaaaga ctatatataa
      961 aaagttatat atatgaccat cacgaccatc acccctgcct aatccctgac gccaaggagg
     1021 taaggcatgg gcgaaaaaca tattgagcaa aagctggtca aagcagtaaa agcagcgggg
     1081 ggcatggcac caaagtttat cagtccgggc cttgccggta tgcctgatcg cctggtgtta
     1141 ctgccaaaag gcaagatggc ttttgttgaa gttaaaagcc ccgggatgaa gcctcgtcct
     1201 ttacaaataa aaagacaccg aatgttacgg cacctaggtt ttaaggttta tgtgctagat
     1261 gatgcaactg agataaaaaa ggtacttgcg gaggtgatgt cagatggagt tcataccaca
     1321 taaatatcag cagtatgcca ctaactatat tcttgaaaat ccggtggcgg tgatattttt
     1381 ggatatgggc ttaggttagc aaaactgtta tcaccctcac ggccatattt gatttgacct
     1441 tggatagttt tatagttcgt aaagttctgg tggtggcacc tttaagggtg gccagagaca
     1501 cgtggccggc cgaaattgag aaatgggatc atctaaaagg tcttacatat tcggttgcca
     1561 ccggtagtga aaaggagcgt aaagcggcac ttatgcaaaa ggcagatatc tacatcatta
     1621 accgggaaaa cgtggactgg ttagttaata gaagcggcct tccttttgat tacgacatga
     1681 tggtcgttga cgagctaagt tcctttaagt cacatcgggc gaaacgcttt aaaagcctga
     1741 tgaaggtgcg gcccaaggta aaaaggattg tgggcctcac cggcacacca tcagccaacg
     1801 gcctaatgga cttgtgggca gagtttcgtc ttttggatat gggccacagg ctgggccgct
     1861 ttattggccg gtatagggag gactactttg tgccggataa acgcaatcag cagattgtgt
     1921 tcagctacaa accaaagccc ggggcagagc aggcaattta caagcgcatc gcagatatca
     1981 ccatcagcat gaagaacaca gactatctga agctaccgga actggtgatg aacgaagtgg
     2041 ccgttaaact gtcggacaaa gagacggatt actaccggac tttgcaacaa gagttggtgc
     2101 tatcgcttaa gggtaaggag attgatgccg taaatgccgc tgccctgtcc agtaaactgc
     2161 ttcagatggc caatggggct gtatatgacg aagatggcag tgtagtggag atacatgacc
     2221 gcaagcttga tgccttagag gatctaatcg aggcgactaa tggcaaaccg gtcttaatcg
     2281 cttactggtt taagcatgat ctggggcgga tattagagag attccccgca gaaaaacttg
     2341 aaagcacaag ctccattaag cggtggaacc aaggggagat cccggtagca gccatccatc
     2401 cagcctctgc aggccatggt ttgaatttgc aagctggtgg ctgcacctta atctggtttg
     2461 gccttacctg gagtctggaa ctttaccagc aaactaacgc ccggctctgg cggcagggcc
     2521 aaaaaaacac ggttgtcatt caccacatta tcacccaaaa caccattgat gaagatgtca
     2581 tggaggcttt aaagagaaaa gataaaaccc aaactgcact tattaaggcg gtaaaagcga
     2641 gaatctatca ggaggtgaag taactatgga agactgcttt gcttacagag gaaatagctg
     2701 cacagccttg aaggttaaaa agtgtgaagg ctgcagtttc tataaaacta aaaagcaata
     2761 cgagctggat caactaaaag caatggaacg aatcctctcc cttgatgcca agcgaaggga
     2821 gtatatcata aacacctatt acaacggcaa actggaggtg ggaacctttg agggttaaag
     2881 aatatttatc ccaagcccta tggctggata aaagaatcaa tagcaagctg gaacaactgg
     2941 aagtgctgag ggcactggcc acaaaggtat cggctaactt aagagaagaa aaggtgtccg
     3001 ggggcaataa tactaaaagc cacctggaga acacagtggt caagatcgtg gatttagaaa
     3061 aagagatcaa tgaggatatt gatcggttgg tggggattaa aacagaaatt atgaatacca
     3121 tcggccaagt ggatgatcct attagtcagc tactattaga aatgagatat gttaacggca
     3181 aatcgtggga tgctgtggcc agggaattaa gattcgacag aagttggatt tcccgtctac
     3241 atggtaacgc cttaaaagaa attgagggta gggtgaaata cgcatcaaaa agcaacaaaa
     3301 agcaataaaa gcaacaagaa gcaacagccg acctgtgcta tactataaga tgtaaaggca
     3361 tggaaaaata tcaggaacac cagatgctgt ttgcataggc cttagggata gatcgaccac
     3421 gactcccggg aaacgcagca ttcttgataa caagcccttg gggattaacc cccccggggc
     3481 tttttctatg cctgaaatta ggaggtgccc tatgaaatgc gtatcctgcc gctttgctgt
     3541 tattgacgaa gcggccagcg atagagattg ggcggcttat gagtgcggca atccaaagag
     3601 cgaatatcat aaatccttaa ttaacataga tgaagaaggt aaaaaacata aacggatttc
     3661 ctggtccggc tgcgaacatg gcgaaaggaa ggtgaaggca aatgccaagg aaacccttaa
     3721 agccttgtcg ctatcccggc tgtccggaac tgacggagga gcggttctgt cccaaacaca
     3781 tgaaagaata caaccggcag tataaccggg aagagagacc ggcgtattct aaaaaactat
     3841 acaaaactgc ccggtggcag cggctaagga aacgggtgct atcaagaaac cccctttgtg
     3901 tggagtgtga gcgtcaagga aggattaccc cggcgatagt tgtggatcac atcatccccc
     3961 acaaagggaa tctgga
//
LOCUS       DASRMS010000107        11247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_95539, whole genome shotgun sequence.
ACCESSION   DASRMS010000107 DASRMS010000000
VERSION     DASRMS010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95539"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1037)
                     /locus_tag="VFC74_05055"
     CDS             complement(<1..1037)
                     /locus_tag="VFC74_05055"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK24739.1"
                     /translation="MAKIIIDNQESEVKGNSTIYEACRQAGIILPALMYDDRLEPVAS
                     CSLSIVKVEGEAELVNACTQVVSDGMKIHTQTEQVKEARKEILISLLAEHPLECENCS
                     TATQCKLQTYCQEYEISSPPQTAKRKEYELDERNPFYTINPNKCIACKLCVRVCHELQ
                     GVGALEMNEEGHVQRIITVECESCGNCIDICPVRAIIPKRFEEEYAQELAVMDNTQAA
                     EETRYVKTTCSYCGVGCQLELVVINNKVVDVKPLKVPPNNGLLCVKGKFGHKFINHSS
                     RLTTPLIKKDGKLVKATWDEAYQLITEKVKEIKEKHGSDVFGGLSSARYTNEENYLFQ
                     KMVRTVFGTNN"
     gene            complement(1112..1981)
                     /locus_tag="VFC74_05060"
     CDS             complement(1112..1981)
                     /locus_tag="VFC74_05060"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007060499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZK24740.1"
                     /translation="MKVPEIMEQRMCFSFEIYPPKDDQPLEPLLDTLEHLYKFEPDFV
                     SCTYGAGGSNAGRNVEIVKAMKERGKTIPVTHYTCIGNTKETITKEMQGYLQIGVDHI
                     LALRGDFPKGWEETRGDFDYANELVEFIRTNFPQFTIAVAGNPEKHFEADSFTADIAH
                     LRIKQDAGADYIMTQLCHDVEQYEWWVEAIRKAGIYLPIDVGVMPVLLKEPTIRMAVM
                     NGSAIPRDLAEIIGRYGNNPEDFKKAGKEYTVKQIHQYINAGIDGLHLYALNKWRDIT
                     DIINDAGIRKTSE"
     gene            2225..2599
                     /locus_tag="VFC74_05065"
     CDS             2225..2599
                     /locus_tag="VFC74_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002605298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK24741.1"
                     /translation="MFFIFGAASKQEQLDFNQPAVCTQCGRYGNYTVIMEYMYFSLFF
                     IPLIKWHKQYYVKSSCCNSVYTITQELGGRIASGGSTTLTEQDLQLVRDGSRETQKCC
                     AKCGYQTTEDFKYCPKCASPLA"
     gene            2961..4091
                     /locus_tag="VFC74_05070"
     CDS             2961..4091
                     /locus_tag="VFC74_05070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24742.1"
                     /translation="MIELRDLVHIYPNGVRAVNNLNLQINDSEICVLIGPSGCGKSTT
                     LNMLNKLIPVTSGEIMIDGHNIEEMVTTELRRNIGYAIQDVALFPHRTVAENIATVPA
                     LKRWSRQKINARVEELLDLMGLEPAQYRDKYPIQLSGGEQQRVGVARALGANPPILLM
                     DEPFGAIDPITRARLQDEFLLIQEKIHKTIVFVTHDIHEAIKMGDKIAIINEGQLVQS
                     GTPKDILQNPANRFVEEFMGEDRHMKWLQLILAEEILEQQNMYCLQTDNYQAVLEKFK
                     QSRLNSLPVVNADQQLIGYVRANVLKNSKQPWDEKIKKYDEWIDKKTTIYDALFALML
                     DVNNYLPIVDKRGKYLGVITQDKVNAILADIKNEVKEVEHVS"
     gene            4081..4728
                     /locus_tag="VFC74_05075"
     CDS             4081..4728
                     /locus_tag="VFC74_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24743.1"
                     /translation="MLAKIIEYVFGNFDYILELIVQHLQVSITAIGLAIAVAVPLGIL
                     LTRYKGMSSFVMGFVGILQTIPSLALLAIMIPFLGIGFKPAVAALFMYSLLPILRNVY
                     TGLNEVTYTMIKAARGMGMTSSQIIFKIQIPIAMPIIIAGIRTAAVICIGTATLAAFI
                     GFGGLGTLIYSGIQQINEVHIFTGAFFSAVLALGVDQLFNLIEYVATPKGLRINK"
     gene            4745..5635
                     /locus_tag="VFC74_05080"
     CDS             4745..5635
                     /locus_tag="VFC74_05080"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK24744.1"
                     /translation="MLKKKLGLISLVLFVIVFALTGCSGGDESAAIRVSNQTWTEASI
                     IGHITEKMITENTDYEVERIELESTVLQWNAINNDQIDIWPDYTSSLFNVFEDDDTVL
                     YEPDAVYDYVLDKAKTDYNLIMLERMGFYNNYDLAVTPEAAAEYDLKTYSDLAAVSDQ
                     LAIAADANFKDREDCYPLLQANYDMNFNEIYQMSVAAKFTAVENGEAEVVACYTTDAG
                     ISRLGLVVLEDDKHANMHFDAVYILRSEILEQYPDLEELLNKVKITNEEMAAMNLQVE
                     GEKRTPDAVAQEFLEEKGLI"
     gene            complement(5762..7231)
                     /locus_tag="VFC74_05085"
     CDS             complement(5762..7231)
                     /locus_tag="VFC74_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007871479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24745.1"
                     /translation="MSNFKDKTINTILDNKVVILFVLLCVAAIYASKNPLTFVASELF
                     TRLGRNGFTVLSLLIPVLAGMGLNFGIVIGAIAAQIAVFWVVYWGYTGIIGFLLCVLL
                     STPIAILFGWLVGRLFNKMKGAEMIAGLVLGYFADGLYQFFFLYVIGGLIPVDNPRLI
                     ISTGVGVKNTIDLADNLKYSLDTVSMLTVLKIAFIAILAVALIKIVLYLVRKTGSLRE
                     NLTLIVAAIVGYAVITLVSPLTAFLATDRLLLLDAVTIGVVGVIAFQAWALISNKLVR
                     KKQNYQISRPLIFIAMAVGIYFATYLKPVEEILLFVKIPVTTYLCIIALCIFNSLILR
                     TRLGQNMRTVGQSQVVANAAGLSVDRLRIIAMIISTVLACWGQLIYLQNMGTFSTYGA
                     HTQVAQFAIAALLVGGASVQKADNKQAIIGVILFHTLFIVAPQAGKELFNNAQLGEYF
                     RVFVAYGVIAVSLAMHAWKTVMKPKEGTLVPGPLNLRDL"
     gene            complement(7233..8291)
                     /locus_tag="VFC74_05090"
     CDS             complement(7233..8291)
                     /locus_tag="VFC74_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24746.1"
                     /translation="MQEQIKKAYDNIGLPRLIIISFFVLMLVFAAPLGLNVASLLGDT
                     LRRWAMYGILVLAMVPAIQSGIGPNFAISLGIVSGLLGATISIELGISNSWVAIGVAM
                     LIGTVMGTILGILYGFLLNNVKGSEMTVSTYVGFSVIAFMNIMWLSLPFKDGNLIWPL
                     GGSGMRNTISLSNTFGELLNKTWGFTIGQEGAGLYVPTGLILFFLFVCVIMWLFMRSK
                     TGIALSAVGSSPTFTRSSGINVNKMRIIGTALSTALGAIGIITYAQSYGFLQLYNAPL
                     WMGFHCVAAILIGGATTKRASIAQVIIGTFLFQGILAVALPVVNQLLPESNLPDVIRL
                     IISNGIILYAISKTKGDK"
     gene            complement(8359..9936)
                     /locus_tag="VFC74_05095"
     CDS             complement(8359..9936)
                     /locus_tag="VFC74_05095"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006774804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HZK24747.1"
                     /translation="MSAQFLLNMQNISKEYDGNQVLKNVTLQVKPGEIHALIGENGAG
                     KSTLMNILFGMPVIHATGGFEGTVEINGEQVSIDSPHQAIDFGIGMVHQEFMLIPGFT
                     VTENIKIGREVSKPTLLSHIFGQELDFLELEKMAQDARKALATIGLNIEEYIKVAGLP
                     VGYMQFIEIAREIDKTGIKLLVFDEPTAVLTESEAERLLEIMKKIAAKGIAIIFITHR
                     LSEVMDYADSMTVLRDGELITRKEIKDTSTIEIAELMIGRKVEKLVDLAEDNRTFTDN
                     SVALSLKGFHVEMPGERVRGIDLEIRKGEIFGIGGLAGQGKIGIPNGIMGLYKSQGTV
                     EVFDKKLELSKLGNAMANAVSFVSEDRRGVGLLLDESIERNIVFSAIQIKNEYLHHLG
                     PIKLLDQKKMRQHALEMIKLLDIRCTGPTQKVGSLSGGNQQKVCLARALTMNPDILIV
                     SEPTRGIDIGAKKLVLEYLVKLNREQGLTIVVVSSELVELRSLCDRIAIVSGGKIATI
                     IKPDAPDAEFGLAMAAG"
     gene            complement(10055..11206)
                     /locus_tag="VFC74_05100"
     CDS             complement(10055..11206)
                     /locus_tag="VFC74_05100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3798 domain-containing protein"
                     /protein_id="HZK24748.1"
                     /translation="MTKKLLALLLIAMMLFSLTACGRGNEGEGEGEGEGEDAAYKIGI
                     MTGTVSQGEEEYQAAQQMKEKYGDKIVTATYPDNFSSETETTIANVVAMASDPEVKAI
                     VFVQAVPGAAAAIDKVRETRPDMLFVAGVAAEDPATIADKADIVMLVDELSMGKTIPE
                     KAAEMGAKTFIHYSFARHLSYATIAARRELMIETCDELGIEFVDVTAPDPTGDAGVSG
                     AQQFILEDVPRQIDEYGKDTAFFSTNCSMQEPLIRSILELGAIYPQQCCPSPYHGYPA
                     ALSVNVSGHEGDVQYMLDSIKEKLVAAGQESRMSTWSVPINMLMIEAGVDYAIEFCEG
                     RTDGRFDEDAFVAAVTKVDNTCQISTYEDGDVKLDNFYMLLCDFYDFSK"
BASE COUNT         3294 a         2316 c         2418 g         3219 t
ORIGIN      
        1 atattgttag taccaaagac tgttctgacc atcttctgga agagatagtt ttcttcatta
       61 gtgtatctag ctgaacttaa gccgccaaaa acatcactac catgtttttc ttttatttcc
      121 ttaacttttt ctgtaattag ctggtaagct tcatcccaag ttgctttgac aagtttgccg
      181 tccttcttga tcaggggagt ggttaatctc gaggagtgat taataaactt atggccgaat
      241 ttgcctttga cgcatagcag accgttattg ggtggtactt tcagcggctt aacatcaacg
      301 accttgttat ttatcacgac taactctaat tggcagccaa cgccgcaata ggaacaggta
      361 gttttcacat agcgtgtttc ttcagccgcc tgggtattat ccatgaccgc tagttcttgg
      421 gcatattctt cctcgaaacg tttagggatg atagcccgta ctggacagat atcaatacag
      481 ttaccacaag attcacattc aaccgtaatg atacgctgta catgaccctc ctcattcatt
      541 tccagagcac caacaccttg caattcatgg caaacccgta cacagagctt acaagcaata
      601 catttattgg ggttgatagt gtaaaagggg tttctttcat caagctcata ttctttgcgt
      661 tttgctgttt gtggggggga ggaaatttca tactcctgac aataagtttg tagtttacac
      721 tgcgttgcgg ttgagcaatt ttcacattcg agagggtgtt cggcaagcag ggaaattaat
      781 atttcttttc ttgcttcctt tacctgctct gtttgagtgt ggattttcat gccatcacta
      841 acaacctggg tacaagcatt gaccagctct gcttcaccct caaccttcac aatgcttaag
      901 ctgcagctag ctaccggttc taacctgtcg tcatacatta aagcgggaag gatgatccct
      961 gcttgccgac aagcctcgta aatagtggaa ttgcctttaa cttcagattc ttgattatca
     1021 ataattattt tggccatctg ctaacctccc tgccgcgtct atgacaagga gggcctgtca
     1081 gaccctcctg aaaattggtc aagatttact attactcact cgtttttcta atgccggcat
     1141 cattaatgat gtctgtaata tcacgccatt tatttagggc atagagatgc agaccatcaa
     1201 taccagcatt gatgtattga tgaatttgtt taactgtata ttctttaccg gccttcttaa
     1261 aatcctccgg attgttgcca tagcggccga taatttcagc taaatctctg gggatagcgc
     1321 tgccattcat cactgccatg cgtattgttg gctctttgag caggacgggc atcacgccga
     1381 catcaatcgg taaatagatg cctgccttgc ggattgcttc tacccaccat tcatattgtt
     1441 ccacatcatg acagagctga gtcataatat agtcagcacc ggcatcttgc ttgatacgta
     1501 gatgggcaat atcagctgtg aaggaatcag cctcaaagtg tttttctggg ttaccggcga
     1561 cagcgatggt gaactggggg aagttagtac gaataaactc caccaactcg ttggcgtaat
     1621 caaaatctcc ccgcgtttct tcccagcctt taggaaagtc cccgcgcaga gcaagaatat
     1681 ggtctacacc gatttgcaaa tagccttgca tctctttggt gatggtttct ttggtgttgc
     1741 caatacaagt ataatgggta accggaatag tcttgcctct ttctttcatc gctttcacga
     1801 tttccacatt acgtcctgca ttggagccac cagcgccgta ggtgcagctg acaaagtctg
     1861 gttcaaattt atagaggtgt tctaaagtat ctaagagtgg ttctagcggt tgatcatctt
     1921 ttggtggata gatctcaaaa gaaaaacaca ttctttgctc cataatttct ggtaccttca
     1981 tcataaaacc cccaaaatta tatttgttgt actgttattt tccctatcaa gggcgctatt
     2041 attacagaaa gctagttgaa aagtttaatt ctgttgtcat gctggtaatg agagttaact
     2101 tttggtaaaa ctatggcgaa acttaactgt tagtgataga atatagccag cgacagcttg
     2161 gcaaattaaa agaaatacta aaagtgaaaa aatagccgat aatatttgtg gagatgagta
     2221 aaagatgttt tttatttttg gtgcagcatc caagcaggag cagctagatt ttaatcaacc
     2281 tgcagtttgc actcagtgcg gcagatatgg taactacaca gtgattatgg aatatatgta
     2341 ctttagtctg tttttcattc ctctgatcaa atggcataag cagtactatg taaaaagcag
     2401 ctgctgcaat agtgtctata ccattacgca ggagcttggt gggcgaatcg ccagcggtgg
     2461 cagtactacc ctgacagaac aagatttgca attggttcgg gatggaagca gagaaacgca
     2521 gaagtgctgt gctaaatgtg gttatcaaac aactgaggat tttaagtatt gcccaaagtg
     2581 tgctagtcca ctcgcttagt taaaaccgtt atctcccatt taccttagcc ggatattttg
     2641 acataatcca agaattgtga taagtatata gagaggaact tatttataaa cacaaacttt
     2701 cgtatatttt acaatatttc atggagccgg ggactcgttt ttgctttaag ttctaattgt
     2761 tataaatttt atactgctat ctggtagtgt actgtaagta gcaatgcata cagaagtacc
     2821 ctaactctat gctctataga gtttaggtac ttttttaaat tgtcgaattg ttgtagtgaa
     2881 gcagcagcag gaggcctgtg aagccatctt ctgtgtgaga gaatggcttc acaggcaaat
     2941 agaaggagga gaaaaacagt ttgattgaat taagagattt agttcatatc tatcccaatg
     3001 gtgtgcgtgc tgtgaacaac ttaaatttgc aaatcaatga tagtgagata tgtgtgctca
     3061 tcgggccatc gggatgcggc aagtctacca ccttaaatat gcttaacaag ttaatacccg
     3121 taacatccgg tgaaataatg atcgatggtc acaatattga ggagatggta actacagagt
     3181 tgcggcgtaa tattggctat gccattcaag atgttgcact tttccctcac cggacagtag
     3241 ctgaaaatat tgcaactgtg ccggcactaa aaagatggtc tagacagaaa attaatgctc
     3301 gtgtcgagga gctgttggac ctaatggggc tggagcccgc acaatacaga gacaagtatc
     3361 ctattcaatt aagtggtggt gaacagcagc gggttggagt agcgcgtgct ttaggtgcga
     3421 atccgccgat acttttaatg gatgaacctt ttggagcgat cgatccgatt accagggcaa
     3481 gattacagga tgaattttta ctaattcaag agaagattca taagacgatc gtgtttgtta
     3541 cgcatgatat tcatgaagcg attaagatgg gtgataaaat tgcgattatc aatgaaggac
     3601 agttagtgca gtcgggtaca cctaaagata ttctgcagaa cccagctaat agatttgtgg
     3661 aagaatttat gggtgaagat aggcacatga aatggctgca gttgattcta gcagaagaaa
     3721 tactggaaca gcaaaatatg tattgcctgc agacggacaa ctatcaggct gtgctggaga
     3781 aatttaagca aagcagactt aatagtctgc ctgtggtaaa tgcagaccag cagcttattg
     3841 gttatgttcg agcaaatgta ctgaaaaata gcaagcagcc gtgggatgag aaaattaaaa
     3901 agtatgatga gtggatcgat aagaaaacta ctatttatga tgctctgttt gccctgatgc
     3961 ttgatgtcaa taattatctg ccaatcgttg ataaaagagg taaatacctt ggtgtgatca
     4021 cacaagataa agtcaatgcg attttggcgg atattaaaaa tgaagtaaag gaggttgaac
     4081 atgttagcta aaataattga gtatgtcttt ggtaattttg attacatttt ggagcttatt
     4141 gttcaacatc ttcaagtatc aattacagct atcggcttgg caattgccgt ggccgtgccg
     4201 ttaggtattc tgctaacccg ctataaagga atgtcaagct ttgttatggg cttcgtggga
     4261 atcttgcaaa ctatccccag cctggcgtta ttggcgatca tgatcccctt tttagggatt
     4321 gggtttaagc cagctgtggc agctttattt atgtattcat tgttaccaat tctacgcaat
     4381 gtgtatacag gtttaaatga agtcacgtat accatgataa aagctgcccg cggtatgggc
     4441 atgactagtt cacagattat ctttaaaata cagataccta ttgcgatgcc cattatcatt
     4501 gccggcatcc gcactgctgc agttatttgt attggtacgg ccacgctggc agcatttatt
     4561 ggttttggtg ggttaggcac acttatctat tccggtattc agcaaatcaa tgaagtccat
     4621 attttcacag gtgccttttt ctcggcagtt ctggcgcttg gcgtcgatca gctgtttaat
     4681 ctgattgagt atgtcgctac ccctaagggt ttgcgcataa ataagtaaac aaagaggagg
     4741 aagtatgtta aaaaaaaaat tggggttaat ctctttagtg ttatttgtga tcgtttttgc
     4801 actaactggg tgcagtggcg gcgatgaatc agctgctatc cgtgtttcga atcaaacctg
     4861 gacggaagct agtattatcg gtcatattac cgaaaaaatg attacagaga atacagatta
     4921 tgaggtagaa aggattgagc tggaaagtac cgtcttgcag tggaatgcta tcaataatga
     4981 ccagatagat atctggcctg attatacatc gtccctgttt aatgtgtttg aagacgatga
     5041 cacagttctc tatgaaccag atgccgtgta tgattatgtt ttagataaag ctaaaactga
     5101 ttataactta attatgctgg agcgtatggg gttttataat aactatgatt tagctgtgac
     5161 gccggaagca gcggcagaat atgatttaaa aacgtattcg gatttggctg cggtctcaga
     5221 tcagctagcc attgcggccg atgccaactt taaagaccgt gaagactgtt atcctttact
     5281 gcaggcaaac tatgacatga actttaatga aatttaccaa atgagtgtgg cggccaaatt
     5341 cacagctgta gagaatggtg aggctgaggt tgtggcttgt tatacaacag atgctggaat
     5401 tagccgcttg gggttagtag ttctagaaga tgacaaacat gccaatatgc attttgatgc
     5461 tgtctatatc ttacgtagtg agattttgga gcaatatcct gatttagaag agctgctaaa
     5521 caaggtaaaa attacaaacg aggaaatggc ggcaatgaat cttcaggttg aaggcgagaa
     5581 gcgcacaccg gatgctgtag cccaggaatt ccttgaagaa aaaggcttaa tctagttaaa
     5641 gacaaataaa attaaataaa aaaacagcaa gactggaggc ttacccctct atgatagaat
     5701 aaaatacctt cgaccaagtt taactggtcg aaggtatttt tgtggtaact tttagttgct
     5761 gctacaaatc tcgtaggtta agtggccccg ggactaatgt accttctttg ggtttcatta
     5821 ctgttttcca agcgtgcatg gcaagggaaa cagcgataac accgtatgct acgaacactc
     5881 tgaagtattc acctaactgg gcattattga agagctcttt gccggcttgc ggggcaacaa
     5941 tgaagagtgt atggaagagg ataaccccaa tgatcgcctg cttgttgtca gccttttgta
     6001 ccgaggcccc gccaactaac agagcggcaa tggcaaattg ggcgacttgt gtatgagctc
     6061 cataggtgga gaatgtaccc atgttttgca agtaaattag ttggccccag caggcaagga
     6121 cagtggagat gatcatcgca atgattctta agcgatccac actaagacca gcggcattcg
     6181 ccacaacttg gctttgtccg actgtgcgca tgttctgacc gaggcgggtg cgcagaatga
     6241 gggaattgaa gatacaaaga gcgatgatac agagataagt cgtgacagga atttttacaa
     6301 aaagcagtat ttcctccacc ggttttagat aggtggcaaa atatattccg acagccatag
     6361 ctataaaaat aagtggacgg gaaatttgat aattttgctt tttcctaacc agcttattac
     6421 taatcagagc ccaggcctgg aaagcaatta ctcccacaac gccgatggtc actgcatcca
     6481 gcagcaacaa acgatcggtg gcgagaaaag cagtaagggg agaaactaag gtaatcactg
     6541 catagcctac aattgctgca actatgagcg tcagattttc acgtaaagaa ccggtttttc
     6601 gcaccaagta caatactatt ttaattaagg ctactgccaa tatggcaatg aaagcaattt
     6661 tgagaactgt gagcatggaa acggtatcta aggaatactt taggttatct gccaagtcaa
     6721 tggtgttttt cacacctaca ccagtggaaa taatcagccg tgggttatct acaggaatga
     6781 gaccaccaat aacataaagg aaaaagaatt gatacaagcc gtcggcaaaa taacctaata
     6841 ccagaccggc aatcatttct gcacctttca ttttgttaaa taagcgacca accaaccaac
     6901 cgaaaaggat agcaatcggt gtggacagca gcacgcagag caggaagccg atgatgccgg
     6961 tatagcccca gtagactacc cagaagacag caatctgcgc agcaatagcg ccaatgacaa
     7021 taccgaagtt tagacccata ccggctaata ctggaatcag gagggacaaa actgtaaagc
     7081 cattgcggcc aagacgtgta aagagttcgc tcgccacaaa agttagcgga ttcttggagg
     7141 cgtaaattgc ggccacgcac aataacacaa agagaattac taccttatta tcaaggattg
     7201 tattaatagt tttatcttta aagttactca ccttatttat ctcctttcgt cttagagata
     7261 gcgtagagga taataccgtt ggagatgatt aggcgaatga cgtccggcaa gttgctctcc
     7321 ggtagtaatt ggttaacgac aggcagtgct acagctaaaa ttccctggaa taaaaaggtg
     7381 ccaataataa cttgagcaat tgatgctctt ttagtggtag ccccaccgat taaaatagca
     7441 gccacgcagt gaaagcccat ccagagaggc gcattataaa gctgcaaaaa accatagctt
     7501 tgggcataag taatgatgcc aatagcacct agtgctgtgg aaagtgcagt gccgataata
     7561 cgcatcttat tgacattaat acctgatgaa cgtgtgaaag tagggctgga acccactgca
     7621 ctcagtgcga tacctgtctt acttctcata aacaaccaca tgattacaca gacaaagagg
     7681 aaaaataaaa tcagtcctgt tggcacatag agacctgctc cctcttggcc aatggtaaag
     7741 ccccacgtct tatttaataa ttcgccaaag gtgttgctta aactaatggt attcctcata
     7801 ccgctgccac ctaggggcca aataaggtta ccatctttga agggcaagga gagccacata
     7861 atattcataa acgcaatcac ggaaaaacct acatatgttg agactgtcat ttctgaaccc
     7921 ttaacgttat ttagcaaaaa cccgtataga atacctaaaa tagtgcccat aactgtacca
     7981 atcaacatag ctaccccgat ggccacccaa gagttgctaa taccaagctc aatactaatc
     8041 gttgctccta acaggccgct aacgatacct aaggagatag caaaattagg tccaatgccg
     8101 ctttggatgg ctggtaccat cgctagtact aaaataccat acatagccca cctgcgcagc
     8161 gtgtccccta agagactggc aacgtttagt cccaagggtg cagcgaaaac taacatcagg
     8221 acaaaaaagc tgatgatgat taagcgaggc aaaccaatgt tatcataggc tttttttatc
     8281 tgttcttgca taatgtatac ctctttcttt agacctcaaa aagtttgatt tagatttatt
     8341 gttgtcagcg tatactcctt aacctgcggc catcgctaaa ccaaattcag catctggtgc
     8401 atcaggctta atgattgtgg caattttacc gccagaaaca atggcaatcc gatcacaaag
     8461 cgaacgcaat tcaaccaatt cagagctcac aactacaatc gtcaaacctt gttcgcggtt
     8521 taatttaaca aggtattcca ggactagttt cttcgcacca atgtcaatac ctctggtagg
     8581 ttctgaaacg atcaggatat ctggattcat cgtcagcgct ctggcaaggc agactttttg
     8641 ttggttaccg ccagaaagac tgcctacttt ttgtgtagga ccggtgcagc ggatatcgag
     8701 taatttaatc atttccagtg catgctgacg catttttttc tggtcaagca gtttgatggg
     8761 gccaagatgg tgtaagtatt catttttgat ctggatggcg gaaaagacga tattacgttc
     8821 tatagattca tcaagtagca ggccaacccc tcgtctgtcc tctgaaacaa aggacacagc
     8881 gttggccatg gcattgccta gcttggaaag ttcgagtttc ttgtcgaaaa cttcaacagt
     8941 accttgtgat ttgtataaac ccataatgcc gttaggaatg ccaattttac cttgtccagc
     9001 cagaccaccg ataccgaaaa tttcaccttt gcggatttct aaatcaatcc cgcgcactct
     9061 ttctcccggc atctcaacgt gaaaaccttt aagcgataaa gcaacgctat tatctgtaaa
     9121 ggtacgatta tcttctgcta aatctactaa tttttcaacc ttacgcccaa tcattaattc
     9181 agcaatttct attgtactgg tatctttaat ctctttacgc gtaattagtt caccgtcacg
     9241 tagtacggtc atgctgtcag cataatccat aacttcggat aggcggtgcg ttataaaaat
     9301 tatcgcaatg cccttagcgg caattttttt cataatctcc aataatcttt ctgcttcact
     9361 ttcagtaagt acagcagttg gttcgtcaaa tactaataat tttatgccag tcttatcaat
     9421 ttctctggca atttcaataa actgcatgta acctacgggt aatccggcta ctttaatata
     9481 ttcctcaatg tttagaccaa tagtggcaag tgctttgcgt gcatcttgcg ccattttttc
     9541 cagttctaaa aaatccaatt cttgaccaaa gatatgactt aacagtgtgg gcttactaac
     9601 ctctctaccg attttgatgt tttctgtgac agtaaaccca gggattagca taaattcctg
     9661 atgcaccata ccaatgccaa aatcaatggc ctggtgcgga gaatctatac ttacctgttc
     9721 accgttaatt tccacagtac cttcaaagcc accggtagcg tgaataactg gcatgccaaa
     9781 aaggatattc attagggtgg atttgccagc accattctca ccgatgagag cgtggatttc
     9841 gccgggtttc acttgtaagg taacattttt taaaacttgg ttaccgtcat attctttgga
     9901 gatgttttgc atgtttaata aaaattgagc actcaaatgt acacccccgc acatgtggta
     9961 aaaaatataa aaatcaaaaa aataataaaa aataaaatag atacgccagc ttgccgagca
    10021 agccggcgta tcatttagcc gatagcttta gttattattt actgaagtcg tagaaatcac
    10081 aaagcagcat gtagaagttg tcaagtttga catcgccatc ttcgtaggtg ctgatctgac
    10141 atgtgttgtc aactttggta actgctgcaa cgaaagcatc ttcatcaaat ctaccgtcgg
    10201 ttctgccttc acagaactcg atggcataat caacgccagc ttcgatcatt aacatgttaa
    10261 tgggcacaga ccaagttgac attctgcttt cttgaccagc tgcaaccaat ttttccttaa
    10321 tggaatcaag catgtattga acatcacctt catgaccgga cacattaacg ctcagagctg
    10381 caggatagcc gtggtaaggg ctggggcagc attgttgcgg ataaatagcg cctaattcca
    10441 aaatggagcg aatcaaaggt tcttgcatcg aacagttggt agagaagaaa gcagtatctt
    10501 taccatattc gtcgatttga cggggtacat cttccagaat gaactgttgt gcaccggaaa
    10561 caccagcgtc accagtgggg tctggagcag ttacgtcaac aaattcgatg cccagctcat
    10621 cgcaagtttc gatcattaac tcacgacggg cagcaatagt agcataggaa aggtgacgtg
    10681 caaaggaata gtgaatgaat gttttggcac ccatttcagc agctttctcg ggaattgttt
    10741 tacccatgga aagttcgtct acgagcatga caatgtcagc tttgtcagca atagtggcag
    10801 gatcttcagc agctacgcca gctacaaaga gcatgtctgg acgcgtttca cgtactttgt
    10861 cgatggcagc agctgcacct ggtacagcct gcacaaagac aatagctttt acctcaggat
    10921 cagaggccat agcaacaacg ttagcaatgg tagtttctgt ttcggaagag aagttgtcag
    10981 ggtaggtagc tgtaacaatc ttgtcaccat atttttcttt catctgttgt gcagcttgat
    11041 attcttcctc accttgcgaa actgtacctg tcatgatgcc aatcttgtag gcggcatctt
    11101 ccccttcccc ttcaccttca ccttcacctt cgttgccccg accacatgct gttaaagaaa
    11161 ataacatcat ggcaatcaag agcagagcaa gcaatttctt tgtcatcggt ttttcctcct
    11221 ttttctctat atgtagaata tttaata
//
LOCUS       DASRMS010000108         1152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_95584, whole genome shotgun sequence.
ACCESSION   DASRMS010000108 DASRMS010000000
VERSION     DASRMS010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95584"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            425..1054
                     /locus_tag="VFC74_05105"
     CDS             425..1054
                     /locus_tag="VFC74_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24749.1"
                     /translation="MTIKNIRQFNSILTSVGNVEQHYTDLLTFAVGNIHRHGNKDPMI
                     SFLNAPFIRTKAGKVKKAYAPLLQWINAACPALTIGERADDKVVKFNATTLPAKVDGF
                     NVLFDKQKEVTVTPYTDWLTEQAADKEPAAPATTVTANALVKYLDSKLALSLTASDQS
                     EIDALVKAAKDLIVAAGKATVPDVDLIRVEELANTKASGAEKTANKKAV"
BASE COUNT          332 a          264 c          250 g          306 t
ORIGIN      
        1 atataccagc tctgggtaaa gtcttcggcc gtgcttttca cggaagaaaa gaatcgctat
       61 atcgtgctgc catatagtag cgcgcattac cttggtttgc tgtcttgcct tagttggttt
      121 gactgtatgc cgtaaaatac aacaggatga aacaagttta gctggttgcg tgttctttaa
      181 taatttgttt agttttttga attgtgcgct ttactggcaa cactgccaac gggacttaag
      241 attcttggtt ttaacgctta ctaacggcgc ggccacgtaa accaacggcc atttattctt
      301 ttgtcttgtg cgtattattc taattttatc taaactttaa ctatatgctt tctagtctaa
      361 taaactagaa agcattcgga taaagtttaa actttatcta ttttaattta ttggggtata
      421 taacatgact attaagaaca ttcgccagtt taattctatc ctgaccagtg ttggcaatgt
      481 agaacaacac tacactgatt tgttaacctt tgcagtgggt aatatccacc gccacggcaa
      541 caaagatcct atgatttcgt ttttgaacgc gccatttatc cgcacgaaag cgggtaaagt
      601 aaagaaagcc tacgccccac ttttacaatg gattaacgcg gcatgcccag cgttaacgat
      661 tggggaacgt gcagatgata aagtggtaaa gtttaacgct accactttac ccgcgaaagt
      721 agacgggttt aacgtgctat ttgataagca aaaagaagtc acagtgaccc cttacactga
      781 ttggctaaca gaacaggcag cagacaaaga gccagccgcg ccagccacca ccgtgacggc
      841 taacgcattg gtaaaatacc tagatagcaa gcttgcttta agcttaacag caagcgatca
      901 atcggaaatt gacgcactgg tcaaggccgc caaggactta atcgtggcgg ctggcaaggc
      961 tacagtgccc gatgttgacc tgatccgagt agaagagctg gccaacacca aggccagcgg
     1021 agcagagaag acggcaaaca aaaaggccgt ctaacccaaa ccgaccagat cactgatctg
     1081 gtcggttttt tttttttcgc gcacccgcca taggtcggcc tatgccccgc gccccgacca
     1141 caacaacaaa aa
//
LOCUS       DASRMS010000109        16534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_431761, whole genome shotgun sequence.
ACCESSION   DASRMS010000109 DASRMS010000000
VERSION     DASRMS010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 16534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16534
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_431761"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            642..1655
                     /locus_tag="VFC74_05110"
     CDS             642..1655
                     /locus_tag="VFC74_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK24750.1"
                     /translation="MRSFKHKRNLSLLFTVFLLVAVVSTGCTGGAGNEADALPTIGII
                     QIREHPSLNTIREEMLAELAAKGYKDGENITIDYQSAQGDPTNLKTISQKFVADGYDL
                     IIAIATPSAQAVVGETSDIPIIFSACTDPVGSGLVASMQNPGGNVTGTCDAVSAEKIM
                     GLALEITPEIKTIGALYNAGETNSVAVINDLKAFAADNDLTVVDATVTNTSEVQQAIT
                     SLVDKVDAVFTPIDNTVASAMLLVAQVAIQAELPVYVGADSMVADGGLATYGINYKVL
                     GQRTGQMVAEVLEGTNPGELAVVTIEDVEIYINEETAERIGVEVPQEILAQANLIKTS
                     LFH"
     gene            1692..2609
                     /locus_tag="VFC74_05115"
     CDS             1692..2609
                     /locus_tag="VFC74_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24751.1"
                     /translation="MSLLAFLGSVELGLIYAVMTLGVFISFRTLNTPDLTVDGSFVLG
                     AAVAAVLCSGGGNPLLAILAAFAAGCAAGAVTALLNTKLQIQPLLSGILVMLALYSVN
                     LRVMGGRANIHLMNAPSIYRLLPKTALNGYSKLATAALALLITLLLLYFFLKTRLGFA
                     LRATGDNEVMVRAAGIDSNQMKLFGLSLANGLVAASGAMIAQYQSYADVGMGIGMVVI
                     GLASVIIGEVIFGVRPLFRRLIAVVLGSILYRLIIAFALGRGMPATDLKLVSAIIVTI
                     ALSTGILKESFAKRVKHLPYFQKRVFHHD"
     gene            2602..3396
                     /locus_tag="VFC74_05120"
     CDS             2602..3396
                     /locus_tag="VFC74_05120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK24752.1"
                     /translation="MISITGLSKIFGKGTINEKIALDNVYLSLAAGEFVTVIGGNGAG
                     KSTLLNCIAGVYPVDAGQIVLNEVDITEMPEFKRSRWLGRVFQDPALGTAFDMTIEEN
                     LAIAYGKNKKRGLQPGITKKEMQYFSEQLVLLELGLEDRMKDKVGLLSGGQRQALTLL
                     MAALVQPKLLLLDEHTAALDPVTAKKVLSLTKQIVKEHQLSTLMITHNMQEALDVGTR
                     TIMMYNGQIMLDLQGEERAKMTVEKLVNQFEQRSGSYLTNDRMLLN"
     gene            3604..5238
                     /locus_tag="VFC74_05125"
     CDS             3604..5238
                     /locus_tag="VFC74_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK24753.1"
                     /translation="MKIIIVGGVAAGASAATKARRVNEQAEIIILEKGPYVSFANCGL
                     PYYVGGDIAKKDNLLLVTPELFQQRFRIEVRLQHEVLAIDAQAKTLTVQSAAGNYAEG
                     YDKLVLATGAEPARPPLPGIDLPQVFTVSTVPDAEAIVNALTAGVQSAVIVGGGFIGL
                     ETAEALLKKGIKTTLVQRGKQLANNFDAEFSLPLEKHLQEMGLDIVLGDSLIKILGEE
                     SVEAVELGDGRQLSTDMVIMAVGVKPRIQLAQSAGLQLGQTGGVLVDASMQTTDPSIY
                     AAGDLVEALHLVSRKKVRIPLAGSASKQGRVAGANAAGGKLLFKGVLGTAIIKVGELT
                     VARSGLSEKEAEELGIDYFVAYTPAGSHAAYYPGSQGMIIKLTAESITGQLLGAQVIG
                     RRGVDKRIDVLATAIYARLSVFDLENLDLAYAPPYGAAKDPVIMAGMIAANMVRGDVT
                     QITPQQLRELQAQPGVIVLDVRTPQEFSKGAVAGAINIPVDELRMRYQELDSAKKVLV
                     YCRTGYRSNLAHLFLRQQGFNASNVSGGYLAYSMDV"
     gene            complement(5324..5497)
                     /locus_tag="VFC74_05130"
     CDS             complement(5324..5497)
                     /locus_tag="VFC74_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24754.1"
                     /translation="MAKSTTTSWAVTGTGAVVTGVGRVVGGRVGAWISGFGLAHIVLG
                     LLDMRRPTIREHF"
     gene            5608..6258
                     /locus_tag="VFC74_05135"
     CDS             5608..6258
                     /locus_tag="VFC74_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011461702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HZK24755.1"
                     /translation="MCGRFTLINWQELIVRFGVAAYDLQPRYNIAPTQGIPVIVQGAK
                     ELRLFRWGLIPFWAADETFAARLINARAETVLEKKSFRHCMQQKRCLIPADGFYEWKK
                     EGNKKQPYYFSLTGGQLFAFAGLWDKWRAPDGSEIFTCTIITTQANQLVAEIHQRMPV
                     ILPPEAEAMWLDPQLRQPEVLQSLLQPYPAAMIRRAVSPLINRPNFDGPEVLNFQA"
     gene            complement(6271..6681)
                     /locus_tag="VFC74_05140"
     CDS             complement(6271..6681)
                     /locus_tag="VFC74_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003378865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK24756.1"
                     /translation="MNIAFFLIPKQEVVFLPLTCTMRQAIERMEYHRYTAVPLVDHQG
                     KYAGTITEGDLLWKMKNTPGLSFENTEKIPITEIPRRVKNEPVRIKAQIDSLLTLAVT
                     QNFVPVVDDNDIFIGIIRRREIIEYFTQQSPRTI"
     gene            7089..8006
                     /locus_tag="VFC74_05145"
     CDS             7089..8006
                     /locus_tag="VFC74_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24757.1"
                     /translation="MAQYVVKRIALAVVTLIIVSTFTFFLMFLVPGGPFLAEKAPSAA
                     TLKALEEKYGLDQPIPIQYKNYMLRLLQGDLGTSLKQRGREVNNIIFTRLPVSAKIGG
                     LAILLAVVVGVFLGSVAALNRGKTIDSVIIFFSTCGIAVPGFVVCTVLMYFLGAKLNL
                     LPTFGLTSYKHYIMPVIALAFYPMSYISRLMRSCMLDVLGQDYMRTAKAKGLSQWKAL
                     FKHALRNAILPIVTYLGPLLAYTLTGSFIVEKIFTIPGLGNSFISSITGRDYPLIMGT
                     TIFLAFLIITMNVVVDIIYKLVDPRIKLS"
     gene            8022..9101
                     /locus_tag="VFC74_05150"
     CDS             8022..9101
                     /locus_tag="VFC74_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006861415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24758.1"
                     /translation="MNQKQPFAINMNPDQKVPLSFHLNPEDFAPASDSEKLELVTMRE
                     SVSFWGDGIRRFRKNKLAMASLVVVIFMMFFAFVLPRFYPYNYAQQIRGSEALAPFEY
                     SAPEQAAKDAGEKIFPHMLGTDTLGRDIMARVMMGSSVSILVGLVASTIILIIGSIYG
                     AMAGYFGGWVDTIMMRLVDIIYTVPDILIIILLSVTLKHPLQALATKPGFAWINTVGV
                     GLISIFIVFSLLYWVGMARIVRGQVLALKELEFVSAAKALGANHNRIIRKHLLTNCIG
                     TLIVTTTLQIPSSIFTESFLSFLGLGVSAPMPSLGSLAATALDGIYSYPYRLFAPAFT
                     ISVIILAFNLLGDGLRDAFDPKLKN"
     gene            9113..10120
                     /locus_tag="VFC74_05155"
     CDS             9113..10120
                     /locus_tag="VFC74_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009323905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24759.1"
                     /translation="MSEYLVNIQNLKLSFFTPAGEVKALNDVTIQMKQGDVLGIVGES
                     GSGKSVTAYSLMGLVPEPGRIISGSIEFDGHPMHTMTEKELRQIRGNEISIIFQDPMT
                     SLNPVFTVGNQIEEVILLHTDKNRKQARERAEELLNLVGINEPRKRLRQYPHELSGGM
                     RQRVMIAIALACEPKLLIADEPTTALDVTIQAQILELMNDLKEKIGMSIIMITHDLGV
                     VASMCEKIAVMYAGKIVEYGTADEIFYNPHHEYTKGLLLSIPKLTATDHEKLIPIEGT
                     PVDMLMPPKGCPFAPRCNSCMKICLKEMPPYTDVNDDHYSACWLLQKQAYKMIKDGVA
                     R"
     gene            10117..11082
                     /locus_tag="VFC74_05160"
     CDS             10117..11082
                     /locus_tag="VFC74_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018305960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24760.1"
                     /translation="MNKLVEVQNLKQYFPAGSNSLFNKRFIKAVDDVSFSINKGETLG
                     LVGESGCGKTTTGKSTIRLHEPTAGKIIYDGKDITKVNMLPYRRKMQIVFQDPYASLD
                     PRMTVSDIVGEAIDIHKLASSKAERKDKVITILERVGLNSEHANRFPHEFSGGQRQRI
                     GIARSLAVDPEFIVCDEPVSALDVSIQAQIINMFEDLQDEMGLTYLFIAHDLSVVKHI
                     SNRIGVMYLGKLVELADSLELAFHSLHPYTKSLVSAIPVPDPRISRQQKRIVLEGDVP
                     SPLDPPSGCRFRTRCQYATKICAEQEPEFKEVASDHFVACHHCCE"
     gene            11145..12761
                     /locus_tag="VFC74_05165"
     CDS             11145..12761
                     /locus_tag="VFC74_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HZK24761.1"
                     /translation="MKKLSFLLALMMLVGGVLVGCGGGNTDGEGNGDNAEVVLPVHVG
                     TNPDTIDPALNSAVDGATLIIHGFEGLYILDKDGVPTPGQAETVDISDDGLTYTFHLR
                     DGLKWSDGSDLTAEDFVYSWNRAIAPETAADYAYMFESIKGFADGELDVTAVDAKTLE
                     VNLVAVTPYFLELTAFPTFSPVQKATVEANGEQWALAPETYVGNGPYKLVEWTPSANM
                     VYEKNENYWNVAALGPDKINFILMEDANAVLAAFETGEILFGDDIPTEEIDAWRDKDE
                     FYIEGQLGTYYVSFNTQKAPFDDPLVREAFSLAIDREFICKEIGKGGQQPASAFVAIG
                     LSDADPTKEFRDVGGDYYDPTAAGFEANLARAKEALKEAGYENGEGLPAVEYLYNVEG
                     DGHRLIAEALQQMWGELGVEVTLGSQEWATFLNTRKNGDYQIARNGWLGDYNDPISFL
                     DMWITSSGNNDAQWSNAEYDKLIADVKASSDQAERFEKMHKAEDIIFEESMLAPIYYY
                     VDLYLMSNKLQNAYSSPLGYKYFMFATVAE"
     gene            12896..13192
                     /locus_tag="VFC74_05170"
     CDS             12896..13192
                     /locus_tag="VFC74_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HZK24762.1"
                     /translation="MTNQQDTAGSRVIVSVLGVDRVGIIAAVANILADNNVNILDISQ
                     TTMQELFTMILIADMEHSTIDLAALKEQLAQLGQKLGLKIDAQHENAFRYMHRL"
     gene            13208..14566
                     /locus_tag="VFC74_05175"
     CDS             13208..14566
                     /locus_tag="VFC74_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HZK24763.1"
                     /translation="MLTMQEIMETIKMVQEEHLDIRTITMGISLLDCADSNAKQACRK
                     IYDKITRLAGNLVSVGESLAREFGIPIVNKRISVTPIALVAETAAVDNYVAFAQTLDR
                     AAAEVGVNFIGGFSALVHKGFTTGDQILLNSIPQALAETERVCASVNVATTRAGINMD
                     AVYQMGKIVKETAALTADRDGLGCAKLVVFANVPEDNPFMAGAFHGVGEPECVINTGV
                     SGPGVVKNAVERVSGADFGTLSETIKKTAFKITRMGELVGRAAAERLGVPFGIVDLSL
                     APTPAVGDSVAEILEAMGLERCGTHGTTAALALLNDAVKKGGAMASSYVGGLSGAFIP
                     VSEDAGMIRAVVDGALTLEKLEAMTCVCSVGLDMIAVPGETSAETIAAIIADEAAIGV
                     INNKTTAVRIIPAPGKVVGDSVEFGGLLGRAPVMPLNKYASTDFVRRGGRIPAPIHSL
                     RN"
     gene            14778..14930
                     /locus_tag="VFC74_05180"
     CDS             14778..14930
                     /locus_tag="VFC74_05180"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HZK24764.1"
                     /translation="MGQHILAEFYGCPAEILNDRQRIERLMVEAALETPFNIFEHEVF
                     VKAANF"
     gene            14949..15863
                     /gene="speE"
                     /locus_tag="VFC74_05185"
     CDS             14949..15863
                     /gene="speE"
                     /locus_tag="VFC74_05185"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF002010.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HZK24765.1"
                     /translation="MSAPNWKWFIEYTHPSQAHMHGVEQYIYSGKTKYQVVEIMETDF
                     YGRCLVLDGKIQSSQYDEYIYHETLIQPAMTLHPEPKNVMIVGGGEGAVLREILRHPT
                     IEQIVMVDIDREVVELCKEYLPEWHQGAFENPKVQVHYMDARKYLKKTDEVFDIIFMD
                     LTEPLDDGPSYLLFTKQFFKIASERLADDGIIALQAGSFNPRLIECHAAICNTLKTAF
                     PIVRSYWNFIPSYDSTWGFALAAKTSDPLQLTVKEIDRRLEERSVTKLKYYDGETHQA
                     IFAIPKDIRAAKVKEQRIIADDRPLITY"
     gene            15886..16338
                     /gene="ndk"
                     /locus_tag="VFC74_05190"
     CDS             15886..16338
                     /gene="ndk"
                     /locus_tag="VFC74_05190"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HZK24766.1"
                     /translation="MEKTYVMVKPDGVQRNLVGEIICRFEKRGLKLVGLKLMQIPQEL
                     AAKHYGEHQGKPFYGGLIDFITSGPVVAMVWEGLNAVSVVRTMMGKTNPAEAAPGTIR
                     GDLAMYMGNNVVHGSDSPDSATREINLFFTTDELVDYKKATDFWLNGK"
BASE COUNT         4569 a         3290 c         4014 g         4661 t
ORIGIN      
        1 tagtaagggg aaatctacct gctattaaag gccatacaac gtacctaact aaagcactac
       61 gtggtttgag ttgtccagca taacgtactt ttagggattg gtgaagaaaa actttttccc
      121 tacagtgggt tgacactatc aaaatggaaa atgactcttg accagaggat aatattgtga
      181 taggattgtt aaaaccgtag atggggaagt aaaactacga agcgcttgcg cagcgaatcg
      241 gggatagtgg gagcccgggg aaggcagccg tagacaaatg ggcctctgag ggcggagtga
      301 acgaatttta ttctagtagc tgccgacgtt ttaccagcgt tacttggtag gagtgtgttg
      361 gcactctgta tgagaggatg tcttagtgac atcaatcaag gtggtaccgc aggtagacgc
      421 ctgtccttgt ttagaggata ggtgtttttt gttgccttta aatgccacgc tgttagcgaa
      481 actttaaagg caggcagcac ggaaagggcg ggtaaggaaa ttgaaagtac gcctaatctt
      541 gtgtgtctac cgcttgtggc aagttcatgc tgcggatgaa cttaagacta cagagaagac
      601 acagcgaaaa tctgaagctc ataagaggag gaaattaaag gatgagaagt tttaagcaca
      661 aaaggaactt gtcgttactc ttcacagtat ttttgctggt ggcagtagtt agtacggggt
      721 gtaccggcgg ggcaggaaac gaagcagatg cactgccgac aattggcatc attcagatta
      781 gagaacatcc ctcgctaaac actatccgtg aagaaatgct ggcagaactg gctgcaaagg
      841 gttacaaaga cggtgaaaac atcaccattg actatcaaag tgcccagggt gatcccacaa
      901 atttaaaaac tatcagtcag aaatttgttg ctgatggtta tgatttgatt atcgctattg
      961 ctacaccatc agcacaggcc gtagtagggg aaacaagcga tattcccatt atcttttccg
     1021 cttgcactga tccggttggt tccggactgg tggcaagtat gcagaatcca ggtggcaatg
     1081 tgacaggtac ttgcgatgcc gtgtcggcag aaaaaataat gggcttggca ctggaaataa
     1141 cgcctgaaat caaaactatt ggcgctcttt ataatgccgg ggagacaaac tctgtcgctg
     1201 tgattaatga tttaaaagct tttgctgccg acaacgattt gactgtggta gatgctaccg
     1261 taacgaatac ttccgaggtg cagcaggcaa ttacttcctt agtagataag gtggatgctg
     1321 tttttacccc tattgataac actgtagctt cagcaatgct cttagtggcg caggtggcaa
     1381 tccaggctga actgccagtc tatgtaggag cagattccat ggtggcagat ggtggacttg
     1441 cgacctatgg tattaattat aaagtactgg gtcaaagaac cggccagatg gtggcggagg
     1501 tgctggaagg aacaaaccct ggtgaacttg ctgtagtcac cattgaagat gtggaaattt
     1561 acattaacga ggaaacagct gaacgtattg gcgtagaggt gccgcaggag attttggcac
     1621 aagctaatct gataaaaaca agccttttcc attaaaagta atccaaaaac taaggatata
     1681 ggggatgccc aatgagtttg ctcgcttttc ttggctcagt tgaattaggt ttaatttatg
     1741 ccgttatgac tttaggtgtt tttatttcct ttcgtacact aaatacgcct gatctaacgg
     1801 tagatggaag ttttgtgtta ggcgccgccg tcgctgctgt gctttgcagc ggcgggggta
     1861 accccttgct ggcgatttta gctgcttttg ctgccggttg tgcggctggt gctgtgacag
     1921 ctcttttaaa taccaaactg cagattcaac ccttgctttc aggtattttg gtcatgctgg
     1981 ccttatattc tgtaaaccta cgggtgatgg gtggtagagc taacattcat ttaatgaatg
     2041 caccatctat ctaccgccta cttcctaaaa cagcgctgaa tggttacagt aagctggcca
     2101 cggctgcact agccctcttg attacgctct tgttattgta tttcttctta aaaactcgtt
     2161 taggctttgc tttgcgggcg acaggagata atgaagtaat ggttagagct gccggtatag
     2221 atagtaacca aatgaagttg tttggtttat cccttgctaa tggcctggtt gctgcttccg
     2281 gagcgatgat tgcccagtat cagtcttatg ctgatgtggg gatgggaatc ggcatggtgg
     2341 tcattggttt agcttccgtg atcattggcg aagttatttt tggtgttagg cccctcttcc
     2401 ggcggttaat tgctgtcgtg cttggttcta tactgtaccg tttgataatt gcttttgctc
     2461 taggcagggg gatgccagct acagacttaa aattagtttc ggcaataatt gtaaccatag
     2521 ccttatcaac aggtatttta aaagagagtt ttgcaaagcg agtaaaacat ctaccatact
     2581 ttcagaaacg agtgttccat catgattagt attacaggtc ttagcaaaat atttggcaaa
     2641 ggtacgatta acgagaaaat tgccttggat aatgtttatc tctctttggc agcaggcgag
     2701 tttgttacgg tgatcggtgg taatggtgcc ggaaaatcca cgctgttaaa ctgtattgct
     2761 ggtgtatatc cagtagatgc cgggcaaatt gttttaaatg aagtggacat tactgaaatg
     2821 cctgaattta aacgctcgcg ctggctggga cgtgtcttcc aagatcctgc tttgggcact
     2881 gcctttgaca tgacgattga agagaatttg gctattgcct atggtaaaaa caaaaaacgg
     2941 ggtttacagc ccggtattac taaaaaagaa atgcaatatt tttctgaaca gctggtttta
     3001 ttagagttag gcctggaaga tagaatgaaa gataaagtgg gcttgttgtc aggaggacaa
     3061 cggcaggcgc tcacgctcct gatggccgcg ctcgtgcagc caaaattgct cttgttggat
     3121 gaacatactg cggctctaga tccggttact gccaaaaaag tcttgtcctt aaccaaacaa
     3181 attgtcaagg aacaccagct tagcacgctt atgattaccc ataatatgca ggaagcatta
     3241 gatgttggga cacgtaccat tatgatgtat aatggacaga ttatgcttga cctccaaggt
     3301 gaagagcggg caaagatgac agtggaaaaa ttggttaatc agtttgagca gcgcagtggt
     3361 tcctatctaa ctaatgaccg catgttatta aactagcaat gatggcaaag ggacgttacc
     3421 tttgccattt ttttgttagc ttgagttaat atttaagtta acgatcatat ggtaaaatca
     3481 acttaactag caagctgttg tgcctctgtt agtgaattac ttcgcttgaa ttttagtaag
     3541 ctagttagtg tttgcagaaa aaatatttac atagagaata ttaagtggaa ggtggaataa
     3601 gaagtgaaaa ttattattgt gggcggcgtg gcggcaggtg cctccgcggc cactaaagca
     3661 cggcgggtta atgagcaggc ggaaatcatt attttggaga aagggcctta tgtttccttt
     3721 gctaactgtg gattgcccta ttatgtgggg ggcgatatcg ccaagaagga taatctgctc
     3781 ttggtgacac cggagctgtt ccagcagcgt ttccgcattg aagtgcgtct gcagcacgaa
     3841 gttctggcga ttgatgctca ggctaaaacg ctgacggtac agtctgcagc gggaaattat
     3901 gcagaggggt acgataaatt ggtgttagcc acgggggcag agccagcacg cccgccgctg
     3961 ccgggaattg acttgccgca ggtgttcaca gtctctacgg tccctgatgc ggaagctatt
     4021 gtgaatgcct tgactgccgg ggtgcagtcg gctgtgattg tgggcggcgg ctttattggc
     4081 ctggaaactg cagaagcact gctgaaaaaa ggaattaaaa ccactttagt gcaacgaggt
     4141 aagcagttag cgaataattt tgatgcggag ttttccctgc ccttggagaa acatttgcag
     4201 gaaatgggat tagatattgt tttaggtgat tcgttaatta aaatcctggg cgaggagagc
     4261 gtcgaggccg tagaattagg ggacggcaga cagctgtcaa cagatatggt aattatggcg
     4321 gtcggtgtca agccacgcat tcagttggca caaagtgccg gtttacaact tggtcagacg
     4381 ggtggggtgt tagttgatgc ctccatgcaa accactgatc cctccattta tgccgctgga
     4441 gatttggtgg aagcgctgca tttagtgtca cgtaaaaagg tgcggatccc gttagcgggc
     4501 agtgccagta agcaaggccg tgtggccgga gctaatgctg cgggaggtaa actacttttt
     4561 aaaggtgttc tcgggacagc cattattaaa gtaggtgaat taacggtagc ccgctccggc
     4621 ttaagtgaaa aggaagcaga ggagttgggc atcgattatt ttgttgccta tactccggca
     4681 ggcagccatg cggcatatta tcccggcagc caggggatga ttatcaaact gacggctgaa
     4741 agtattactg gtcagctatt gggggcacaa gttattggca ggcggggtgt ggataaaaga
     4801 attgatgttt tggctacggc catctatgca cgccttagcg tttttgatct agaaaatctt
     4861 gatttagcct atgcgccgcc ttatggtgcg gcgaaagacc ccgtgattat ggctggcatg
     4921 atcgctgcca atatggtgcg gggtgatgtt acacagataa caccgcagca gttgcgggaa
     4981 ttacaagcgc agccaggggt gatagtgctt gatgtacgca cgcctcaaga attcagtaaa
     5041 ggcgctgttg ccggggcgat taatatacct gttgatgaat taagaatgcg ttatcaggag
     5101 cttgatagtg ctaaaaaagt tttggtttac tgccgcaccg gctatcgttc caatctggcc
     5161 catctttttt tacggcagca gggctttaat gcctctaatg tcagcggtgg ttatctagct
     5221 tacagcatgg atgtttaaaa aaaagacaga ggttgttcct ctgtcttttt taagtttgca
     5281 gaaaagtaca tccccggtta ataaccgggg atgtttgggt gaactagaaa tgttcgcgaa
     5341 ttgtcggcct gcgcatgtcc aagagaccga gcacaatgtg ggccaatccg aacccggaaa
     5401 tccaggcgcc aactctcccg cctactacac gtcctacacc ggttactacg gcaccggtgc
     5461 cagtaacagc ccagctagta gttgtacttt tcgccatttt tttgttcacc tccgctactt
     5521 attgttccat cgcgcagttt ttttattaaa ataagatgag aggttttgtg gaagatctct
     5581 taaaaacata aattaaggat gattgtgatg tgtggaagat ttacgctgat taattggcaa
     5641 gaactaatag taagattcgg ggtggcagca tatgacctgc agccacgcta taatattgcc
     5701 cctacgcaag gaataccggt tatcgtacaa ggagctaaag aattacgttt gtttcgttgg
     5761 ggactcatac ctttttgggc agcagatgag acatttgctg cccgcctgat taatgcgcga
     5821 gcagaaacgg tgctggagaa aaaaagcttt cgccactgta tgcagcaaaa gcggtgttta
     5881 atcccagcag atggctttta tgaatggaag aaagaaggga ataagaaaca accatactat
     5941 tttagtctta ccggcggtca gctttttgct ttcgccggct tgtgggacaa atggcgtgct
     6001 cccgatggca gcgaaatttt tacctgtacc atcatcacca cacaggcaaa ccagctggtg
     6061 gcagaaattc atcagcgcat gcctgtgatc ttaccgccag aagcggaagc tatgtggttg
     6121 gatcctcaac tgcgccagcc agaagttctg cagagcttac tccagccata ccctgcggcg
     6181 atgatcagac gcgcagttag cccattaatt aaccgaccaa actttgatgg tccagaggtg
     6241 ttaaactttc aggcctagcg ctggcaagaa tcaaatggtg cgaggacttt gttgagtaaa
     6301 gtactcaata atttcccgcc gtcggataat cccaataaaa atatcattat catccacaac
     6361 gggcacaaaa ttttgcgtta ctgccagagt aagtaaacta tcgatttgtg ctttaatacg
     6421 gacaggttcg tttttcaccc gcctgggtat ttcagtaata ggtatttttt ccgtattttc
     6481 aaaggagagt ccgggtgtat ttttcatctt ccacaacaaa tccccttccg taattgtacc
     6541 ggcatattta ccttgatggt caacgagggg gacagctgta taacgatgat actccatacg
     6601 ttctatcgct tgacgcattg tgcaggtaag gggaagaaaa actacttctt gtttagggat
     6661 gagaaagaac gcaatattaa tggtcattac ccctttccgg ttaaatgata atactaattt
     6721 cccgatcttt atgctattcc ctgctgtaga aaagtgaaca atttgtggag actattatct
     6781 tccttaggta atagttacct gtgacataag gttgacggta gacataaaaa tctgttattc
     6841 tacaatatgg acgatctact gcaaaattag cggtgaatga gatgcttttt agaccggcac
     6901 gaaagggagt actagggcaa gaacggaata tatggtttac agtcgaatat tttttatcag
     6961 caagaaagca gggaaaatat tattaagggc gaatatctat tctaatacgc acttattttt
     7021 ttgtgcctaa ttcgtcatta tttttagtta tgcgatagtg gattgaactt caaggaggat
     7081 ttaacaagat ggcacaatat gttgtcaaac gtatagcttt agcggttgtt accttaataa
     7141 tcgtttcaac ctttaccttt tttctgatgt ttctcgttcc cggcggtcct tttctggctg
     7201 aaaaagcccc ttcagcggca acattgaaag cactcgagga aaagtacggt ttagatcagc
     7261 cgatacctat ccagtacaag aattatatgc tacgtttgct tcagggagac ttaggcacct
     7321 cattaaaaca gcgtggccgg gaagtaaata atattatctt tactaggtta cctgtctcag
     7381 caaaaatcgg tggacttgcc attcttctgg cagtagtggt tggagtgttt ttaggctccg
     7441 tggcggcctt aaacagagga aaaacaatag atagtgttat cattttcttc tcaacctgcg
     7501 gcattgctgt tccaggcttt gtggtgtgta cggtgctgat gtattttctt ggcgctaagt
     7561 taaatctttt gccaaccttc ggtttaacta gttacaagca ctacattatg ccggttattg
     7621 ctttggcttt ttaccccatg tcttatattt ctcgtttaat gcgctcctgt atgttggacg
     7681 tactaggaca ggactatatg cgcacagcta aagctaaagg cttgtcgcaa tggaaggctt
     7741 tgtttaagca tgccttacgt aatgcgatct tacccattgt tacctacttg gggccgctgc
     7801 ttgcttatac cctaaccggc agctttattg tggaaaagat cttcaccatc cccggacttg
     7861 gcaattcatt tattagctcc attaccggtc gggattatcc actgattatg gggacaacca
     7921 ttttcttggc ttttctgatt atcactatga acgttgtagt tgacattatc tacaaattag
     7981 tcgatccaag aattaaatta agttaggtgg tgctgaaaaa catgaatcag aaacagccat
     8041 ttgcgataaa tatgaaccct gaccaaaaag taccgctcag ttttcatctt aatcctgaag
     8101 attttgcacc cgctagtgat agtgagaagc tagaactggt cacgatgcgg gaaagtgtta
     8161 gtttctgggg tgacgggatc agacgttttc gtaaaaataa actagccatg gctagtttag
     8221 tagtagtaat ttttatgatg ttttttgctt ttgttttacc aagattttat ccctataatt
     8281 atgctcaaca aattcgcggt agtgaagcct tggctccttt cgagtattcg gcaccagaac
     8341 aggctgcaaa agatgcaggc gagaaaattt tcccgcatat gctcggtaca gataccctgg
     8401 gaagggatat catggcacgg gtgatgatgg gtagtagtgt ttctattctg gtcggactag
     8461 ttgcctcgac gattattttg atcatcggtt ctatttacgg ggcgatggct ggttatttcg
     8521 gcggctgggt agacaccatc atgatgcgtt tagttgatat catttataca gtacctgata
     8581 ttttgattat tatcttgctt tctgtaacac tgaaacatcc gctgcaggct ttagcaacaa
     8641 aacctggctt tgcctggatt aatacagttg gtgttggctt gatcagcatt tttattgttt
     8701 tctccttgct ctactgggtg ggtatggccc gtattgtcag agggcaagtg ctggctttaa
     8761 aagaattgga gtttgtgtcc gcggccaaag ctttgggtgc aaatcataac cgcattatcc
     8821 gcaagcacct gttaacaaac tgtatcggta ctttaattgt aactaccact ttacagattc
     8881 cttcttctat ttttacggag agttttctta gctttttagg tcttggtgta tctgcaccga
     8941 tgccttcttt aggttcgctg gccgcaacag cacttgacgg tatttattcc tatccatacc
     9001 gtctctttgc accggcgttt actattagtg tgattatttt ggcctttaac ctactagggg
     9061 acggtctaag ggatgccttt gatcccaaac taaagaatta ggggggcagc aaatgagtga
     9121 atatctagtt aacatacaaa acttgaagct ctcctttttt actccggcgg gcgaagttaa
     9181 agcactcaat gatgttacta tacaaatgaa acaaggcgat gtgttaggta ttgtcggcga
     9241 atcaggcagt ggtaaatctg tgactgccta ttcattaatg ggtttagtgc ctgagcccgg
     9301 tcgtattatt tccggctcta ttgaatttga cggtcatcct atgcatacga tgacggaaaa
     9361 agaattacgg caaatacgcg gcaatgaaat tagtattatc tttcaggacc cgatgacttc
     9421 gctgaatcct gttttcactg ttggtaatca aattgaagaa gtaattcttt tgcataccga
     9481 taaaaatcgc aaacaagcaa gagaacgcgc tgaagagcta ttaaatttag tcggcattaa
     9541 tgaaccacgg aagcgtttac ggcagtatcc gcacgaactt tccggcggta tgcgtcaaag
     9601 ggtgatgatt gccatagcct tggcctgcga gcccaaacta ttgatagctg acgaaccaac
     9661 aacggcactt gatgttacta ttcaggcgca gattctggag ttaatgaatg atttaaaaga
     9721 aaagatcggt atgtctatta ttatgattac gcacgatttg ggcgttgtgg ccagcatgtg
     9781 tgagaaaatt gctgtgatgt acgccggtaa aatcgttgaa tatggcacgg ctgatgaaat
     9841 attctataat cctcaccatg aatacaccaa gggattatta ctgagtatcc cgaaacttac
     9901 cgctacagat catgaaaaat tgattcccat tgagggtact cccgttgata tgctgatgcc
     9961 gccgaaaggc tgtccttttg ccccacgctg caatagctgt atgaaaatct gcttaaaaga
    10021 aatgccgccc tataccgatg ttaatgacga ccactatagt gcctgctggt tgttgcagaa
    10081 acaagcgtac aagatgataa aggatggggt tgcacgatga acaaactagt cgaagttcaa
    10141 aacttaaaac aatatttccc ggcaggcagc aattctttat tcaataaacg ctttatcaag
    10201 gctgttgacg atgtttcttt ttcgattaac aaaggggaaa cgctagggct agtcggtgaa
    10261 tctggctgtg gcaaaactac caccggtaaa agcacgattc gtctgcacga acctactgca
    10321 ggcaaaatta tctatgacgg taaggatatc accaaagtta atatgctgcc ctatcgtcgc
    10381 aaaatgcaaa tagttttcca agatccctat gcttctttag atccccgcat gacagtttca
    10441 gatatcgtcg gcgaagctat tgatattcat aaactagctt ccagcaaagc tgaacgtaaa
    10501 gataaagtga tcaccatctt ggaacgtgta ggacttaact ctgagcatgc taaccgtttt
    10561 ccgcatgagt tttccggtgg acaacgtcag cgcattggta ttgcccggtc cttagctgta
    10621 gatccagaat ttattgtctg tgacgagcct gtttcagcct tggatgtctc tattcaggca
    10681 caaattatta atatgtttga agatttacag gatgaaatgg gactaacgta tttattcatt
    10741 gctcatgatc tctctgtagt taagcatatt tctaatcgta tcggtgtgat gtatctgggg
    10801 aaattggtgg agttagctga tagtttagaa ttagcctttc atagtctgca tccttacact
    10861 aaatctcttg tttcagctat cccggtacca gatcccagaa tctcacgcca acagaagcgt
    10921 attgtgttgg aaggtgatgt gccaagcccc ttggatccgc cttcaggttg tcgtttccgt
    10981 acacgctgcc aatatgctac aaaaatatgt gcagaacaag aaccggaatt caaagaggtg
    11041 gccagcgacc actttgttgc ttgccatcac tgctgtgaat agagtttaag ctctctatct
    11101 cagaggctta aatatagatg aaaaaaagag gaggaaaaga aagaatgaag aagctttcat
    11161 ttctactagc attaatgatg ctggtaggcg gcgtgctggt tggctgcggc ggcggcaaca
    11221 ccgatggcga aggcaacggt gacaatgctg aagtagtact gcctgtacac gttggtacga
    11281 accctgacac catagaccct gcattaaact ctgcagttga tggtgccaca ttgattattc
    11341 atggtttcga gggtctttac atcttggata aagatggtgt gccgactcct ggtcaagccg
    11401 agactgtaga catcagtgat gatggtctta cctacacctt ccatcttcgt gacggtctca
    11461 aatggagtga cggttcagat ctgacagctg aagactttgt ttattcctgg aacagagcta
    11521 ttgctccaga aacagctgct gactacgcct atatgtttga atccattaaa ggttttgctg
    11581 atggcgaact agatgtaact gctgttgatg ccaagacatt agaagttaac ttggttgctg
    11641 taactcctta cttcctggag ttaactgcct tcccgacctt tagccctgtc caaaaagcta
    11701 cagttgaagc taatggcgaa cagtgggctc tagctcctga aacttatgtt ggcaacggcc
    11761 cctacaagtt agtagagtgg actccatctg ctaacatggt ttatgagaaa aatgaaaact
    11821 actggaacgt agctgcttta ggtcctgaca aaatcaactt cattctcatg gaagatgcta
    11881 acgctgtttt agcagctttc gaaactggcg aaattctctt tggtgacgac attcctaccg
    11941 aagaaattga tgcttggaga gataaagacg agttctatat tgaaggtcaa cttggcacct
    12001 attatgtcag cttcaatacg caaaaagcac cttttgacga tcctctcgtg cgtgaagcct
    12061 ttagtctggc cattgatcgt gaatttattt gcaaagaaat cggtaaaggc ggacaacaac
    12121 ccgctagcgc atttgttgcc atcggtttat ctgacgctga tcccactaaa gaattccgtg
    12181 atgtaggcgg cgactattat gatcctaccg cagctggctt cgaagctaac cttgctagag
    12241 ctaaagaggc actaaaagaa gctggttatg aaaacggtga agggctacct gccgtggaat
    12301 atttgtacaa cgtagaaggc gacggtcata gactgattgc tgaagccctg caacaaatgt
    12361 ggggcgaact cggcgtagaa gtaaccttag gttctcaaga atgggcaacc ttcttgaaca
    12421 cccgtaaaaa cggcgactac caaatcgcac gtaacggttg gttaggtgac tacaacgatc
    12481 ctatttcctt cttggatatg tggattacca gcagtggtaa caacgatgct caatggtcaa
    12541 atgctgaata cgacaagctg attgctgacg ttaaagcttc cagtgaccaa gcagagcgtt
    12601 ttgaaaaaat gcataaagcg gaagatatca tctttgaaga gtccatgttg gctccaattt
    12661 attactatgt agatctctat ctcatgagta ataaattaca aaatgcctac tcttctccgc
    12721 taggctacaa atacttcatg tttgctactg ttgctgaata gtaaagaggt atctaaagag
    12781 gctgagttta ctcagcctct tttttttact ttccataatg agctttttta tggtaaaata
    12841 gggcgcaagt aagggtcgca gaaaaggtca gtataaatat taaggagcga tgatcatgac
    12901 aaaccagcag gatacagctg gcagccgggt aatagtcagc gtgctaggag tagatagagt
    12961 tggtattata gctgctgtgg ccaatattct cgcggataac aatgtaaata tcctcgatat
    13021 tagccaaact acgatgcagg agctttttac gatgatcttg atcgccgaca tggagcacag
    13081 cactatcgac ttagcagcct taaaggagca gcttgcgcag ttaggacaaa agctagggtt
    13141 aaaaattgat gctcaacacg aaaatgcttt tcgctacatg caccggcttt aagaaggaga
    13201 acaaactatg ttaacaatgc aagaaataat ggaaactatt aagatggtac aagaggagca
    13261 tttggacatt cgcacgatta ctatgggcat tagcctgctc gattgtgcag acagtaatgc
    13321 aaaacaagcc tgccggaaaa tctacgataa aattacgcgt ttggctggta acctggtctc
    13381 agtaggtgag agtttagccc gagaatttgg tattcccatc gtaaacaaac gcatctcagt
    13441 cactcctatt gccctcgttg cggaaacagc agctgtagat aattatgtgg cctttgccca
    13501 gactttagac cgcgctgctg cagaagtagg tgttaatttt attggtggtt tttccgcact
    13561 cgtacataaa ggttttacca caggtgatca aattctgctg aactctattc ctcaagctct
    13621 ggcagaaaca gagcgagttt gtgcatccgt taatgtcgct accacgagag ccggtattaa
    13681 catggatgcg gtctaccaaa tgggtaagat tgttaaagaa acagcagcct taacagcaga
    13741 tcgcgatgga ctggggtgtg ccaagctagt tgtttttgcc aacgtaccag aagataatcc
    13801 ttttatggct ggagcttttc atggtgtggg agaaccagaa tgtgtgatta atacgggtgt
    13861 aagtggccca ggagtagtca agaatgctgt agaaagagta tcaggtgctg attttggcac
    13921 actatcagag acaattaaaa aaacagcatt caaaatcacc cgcatggggg aactggtggg
    13981 acgtgcggca gctgaacgtt tgggtgttcc ttttggcata gttgaccttt ctttggcacc
    14041 gacaccggca gtaggtgata gtgtggcaga aatccttgaa gccatgggtt tggaacgttg
    14101 tggtacacac ggcactaccg ctgctttggc cttattaaat gatgcagtaa aaaaaggagg
    14161 agccatggca tcttcttatg ttggtggtct gtccggtgcg tttattcctg tcagtgaaga
    14221 tgctggcatg atccgggcag ttgttgatgg cgcactcacc ttggagaagc tagaagcaat
    14281 gacttgcgtc tgctccgtcg gcttagatat gattgctgtg ccgggagaaa cctcagcaga
    14341 aacaattgct gcgattattg ccgacgaggc ggccataggt gtgattaaca ataaaactac
    14401 cgccgtcaga attattccgg cgccaggtaa agtcgtaggt gatagtgtag aatttggcgg
    14461 actgctggga agagcacctg tgatgccgct taataaatat gcgtcaacag atttcgtacg
    14521 ccgtggtggt cgtattcctg ctccaataca cagcctccgc aactaaaacg tggtgtcgtg
    14581 tagtctgtgc gggcgaactc gtacagactt ttccgcttca gaggtaatca cggcaagaaa
    14641 aacaacgcat ttgtcagtct aaatagagca attgacattt tgcctgaatt tgttacaatt
    14701 taacttgatg tccaaacggc cagacgttgt catagcgggg caaaaaaagc atcaaggatg
    14761 cttcacaaaa tgaaggcata gggcaacata ttttagctga attttatggc tgccctgcgg
    14821 aaatccttaa tgatagacaa aggattgaac gcctgatggt tgaagcagcg ttggaaacac
    14881 catttaacat ttttgaacat gaagtcttcg ttaaagcagc gaacttctaa ccgaaagggg
    14941 gaagatagtt gagtgcacct aattggaaat ggtttattga atacacacac cccagccaag
    15001 cgcatatgca tggcgtcgag cagtatattt acagcggtaa aacaaaatac caggtagtag
    15061 aaattatgga aactgatttt tacggcagat gcctggtgct tgatggtaaa attcaatctt
    15121 cacaatatga tgagtatatt taccatgaaa cgctgattca acctgctatg acgctgcacc
    15181 ctgagcccaa gaatgtaatg atagttggtg gtggcgaagg ggcagtctta cgggagattc
    15241 tccgccaccc caccattgaa caaatagtta tggtggatat tgaccgggaa gtagttgaac
    15301 tatgtaaaga gtatttgcct gaatggcacc aaggcgcatt tgagaatccc aaagtgcaag
    15361 tacattacat ggatgcacgt aaatatctta aaaaaacaga tgaagtattt gacatcattt
    15421 ttatggatct cactgaacct ttggacgatg gaccgtccta cttactgttt accaaacagt
    15481 ttttcaaaat tgccagtgaa cgtttagccg atgatgggat tattgcttta caagcgggtt
    15541 catttaaccc tcgtttaata gaatgccatg cggctatttg caatacttta aagacagctt
    15601 ttcccattgt ccgttcctat tggaacttca ttccttccta tgattctaca tggggttttg
    15661 ctctcgctgc caaaacctct gacccgctgc agctgacagt taaagaaatt gatcggcgcc
    15721 tggaggaaag aagtgtgaca aaattaaagt attatgatgg agaaacacat caagcaatct
    15781 ttgctattcc caaggacatc cgggcagcta aagtaaagga acaacggatt attgccgatg
    15841 acagaccgct aattacatat tagtagtaaa tgaggagtga caaacgtgga aaaaacttat
    15901 gtaatggtaa aaccagatgg tgtacagcgc aatttagttg gtgaaattat ctgccgcttt
    15961 gaaaagcgag gattaaaact tgtggggctt aaattaatgc agattccgca ggaactggcc
    16021 gccaaacatt acggtgaaca ccaaggcaaa cctttttatg gtggattaat tgactttatt
    16081 acttcaggac ccgtcgtggc catggtttgg gaaggtctaa atgctgtgtc tgttgtgcgg
    16141 accatgatgg gtaaaactaa cccagctgag gctgccccag ggacaatccg tggcgacttg
    16201 gcgatgtata tgggtaataa tgtggtgcat ggttctgatt caccagacag tgcaactaga
    16261 gagattaatc ttttcttcac aactgatgag ttagttgact acaaaaaagc aactgatttc
    16321 tggctgaacg gaaaataagc tacagagcaa agaaaatgca cacctgcggg tgtgcatttt
    16381 aaattggata gaaaattata ttatccttgg gttatttaag agggattccc ttttagtctt
    16441 gcggggtggc agcccctaaa gcacaaacaa gacgcatcat tgagaaaaga gcgcagcgtc
    16501 ttttctgtgc tattctgtaa tcgtatatta acac
//
LOCUS       DASRMS010000110        15541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_900470, whole genome shotgun sequence.
ACCESSION   DASRMS010000110 DASRMS010000000
VERSION     DASRMS010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 15541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15541
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900470"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..372
                     /locus_tag="VFC74_05195"
     CDS             28..372
                     /locus_tag="VFC74_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HZK24767.1"
                     /translation="MAELLEILKSLADETRYQLLKLLLQHDYCVGALAQKLNISESAV
                     SQHLKILRQTGLIKGDKRGYYTHYYVERELLKQAAKDLNQLSHLVPCSTACHQTDKCS
                     RNCEGRGKHEQA"
     gene            359..487
                     /locus_tag="VFC74_05200"
     CDS             359..487
                     /locus_tag="VFC74_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24768.1"
                     /translation="MSKPDCEHPELRPEDGKCSAEQIRKCHGKDKTHPSHAKDNKE"
     gene            564..1535
                     /locus_tag="VFC74_05205"
     CDS             564..1535
                     /locus_tag="VFC74_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK24769.1"
                     /translation="MSTTIIEVNQLSKHFDDFKAVDEISFSVNNGEVFGFLGPNGAGK
                     TTTINMLTGLARPTSGSILIAGEDGIKHIKKVQQLIGIVPDESNLYDEMSGLENLIFC
                     ASLYGLEKQERESRAKQLLDQFGLANTGKRSFKAYSKGMKRKLTIAAGIIHQPQILFL
                     DEPTTGIDVESARQIRKLLQELNHYGTTIFLTTHYIEEAERLCNRVGFIVEGKVVRIG
                     GLSELMQDAQQDNIVEFALESNSTDLQKALEKEFPKTKITKLEDDKIRISSPVRIELM
                     PFMNFFAQAQVPVYEAKVIHPSLEDVFVKITGIELSKMKKEKEGRKK"
     gene            1532..2266
                     /locus_tag="VFC74_05210"
     CDS             1532..2266
                     /locus_tag="VFC74_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014256555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24770.1"
                     /translation="MNKSIAFWSILMKDMKNYYLKPPNISWGIIFPISWTLMFFVRSQ
                     SAVDIRGILPGVMALSILFGTTSMLAVTITFERKSRSFERLLLAPISLNILMLAKTTG
                     AILFGVINAFIPVFFASFLADLSGINWGAAVLSVVLIAVTSTFLGLFIAVSVSEVFEA
                     QTFSNFFRFPMMFLCGLFIPIPNLPAVVRPLSFLLPLTYGADILKTAINHTGFIAPWL
                     SFIVLLAFTLFLFLMSTRNIKKKWIY"
     gene            2469..3284
                     /locus_tag="VFC74_05215"
     CDS             2469..3284
                     /locus_tag="VFC74_05215"
                     /inference="COORDINATES: protein motif:HMM:NF015995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK24771.1"
                     /translation="MLSISGCGLFTQGNDKETLVIGATPWSSTIPPTNIVATILEEHL
                     DYDVQIQEVDVGTVYSGLATGDIDIFVDAWLPNMHGNYMQEYGDRIDEISVSYTEGIL
                     GWVVPTDSKVESIAELNDYRDEFGGQIIGIDAGAGMMETSQQIIDEYDLDYELVEGSE
                     WAMLTEVSRQFDRGNSVLFLGWRPHWMFAEYDLKFLEDPKGLWQQDEVRNLATKGLAE
                     RAPEAVAFLKNWQMPIDDLEDMIRRMELENEDIDVLVAEWLQENESLVQEMLP"
     gene            complement(3397..4281)
                     /locus_tag="VFC74_05220"
     CDS             complement(3397..4281)
                     /locus_tag="VFC74_05220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009066253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline/glycine betaine ABC transporter
                     permease"
                     /protein_id="HZK24772.1"
                     /translation="MPANFGQWLINVFTSLQLVELPLAKWIESLVDWMGLNLIALFDF
                     INLLVGTTFTYIESFILWLPAILIMAVFTYLAWRLNGRGLALFTVAGFFFVGSLGLWE
                     ETMITLALILTATLISVFVGVTLGILAAKNDSVAGVFRPVLDLMQTMPSFVYLIPALI
                     FFGMGSVPAVISTVVFSMPPAVRMTSLGIRQVPADIIEAAKAFGSSPRQLLVDVEFPL
                     ALPTIMAGINQTIMMALAMVVIAAMIGAGGLGSVVLRGIMRVRVGTGFEGGISIVILA
                     IVLDRISQSLGKSKKDFN"
     gene            complement(4271..5470)
                     /locus_tag="VFC74_05225"
     CDS             complement(4271..5470)
                     /locus_tag="VFC74_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HZK24773.1"
                     /translation="MEKLQVKNLYKVFGSDPQRAVSALEQGLSKDEVQKKMGLTVALR
                     DVSFTVNEGEFFVVMGLSGSGKSTLLRCLNRLIEPTQGSVLVDRQDLTSLNDTQLREL
                     RRQKFGMVFQRFALFPHRTVLENVEYGLKLQGVAPDERRKRALEVLETVDLGVWKDKY
                     PDQLSGGMQQRVGLSRALAIEPDILLMDEPFSALDPLIRRDMQEELMDLQARLQKTIV
                     FITHDLDEALKLGDRIAVMKDGEIVQLDTAEGILTSPANDYVAAFIRGVNRAHVLTAE
                     NIMQKPDALITTKDGPRVALRKMAEARISSVFVVNRNRELKGLITAESAIEAIKEHKE
                     MEKVLTTDLLTTAPDTQVFDLIPMATRAKFPLAVVDDKHRLLGIIVRVSVLSGLVDAE
                     EDKNDAS"
     gene            complement(5725..6036)
                     /locus_tag="VFC74_05230"
     CDS             complement(5725..6036)
                     /locus_tag="VFC74_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006565539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HZK24774.1"
                     /translation="MILVNTDYIAGKDLEMLGLVKGSTIQTRNAGRDITQFFKHLVGG
                     ELKEYNKMMNEARALATKRMMEDAAALGADAVVNIRYASTAIMSGAAEVMAYGTAAKF
                     K"
     gene            complement(6017..6250)
                     /locus_tag="VFC74_05235"
     CDS             complement(6017..6250)
                     /locus_tag="VFC74_05235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013378883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24775.1"
                     /translation="MVMGKQAKKIIAPVIVTLIVVLYFAFISRMSLLQPLPLMARILA
                     IAIPLALISVSIYVLIERIKEIRSGEDDDLSKY"
     gene            complement(6493..8238)
                     /locus_tag="VFC74_05240"
     CDS             complement(6493..8238)
                     /locus_tag="VFC74_05240"
                     /inference="COORDINATES: protein motif:HMM:NF017043.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24776.1"
                     /translation="MSKRYRRYIYFTSFVLACYLFAVLYAAYLQGTVFYYGLVLLLPG
                     GALIWYLLGKMRYAAIVEELAAMWGKASKRDRDFRDLTRMSTLFPVQTQTETIISPQT
                     KEDLHIDLLYSLVDRTLTTPGAQVLYNMLQRPLFDTERLAQRGEMIRLFDTNSPLRKK
                     LRIRLRQLNRQDADTVTNLIWDEEMQPASLGYLPTVLACLALVAVVSPFIIGFKGVFY
                     GIFPMFAINFLYTHKKSARMMISLPAIRYMNSFILAAGDLAQLTAESLPDYAAELAAG
                     VQQCKGLLRKTHHNILRLLDAFGLYDYVNYLFLLELRRFEASMVIISRKREHLQQLYS
                     LVGELDALLAVTSLRAGQAAWVEPELVTEQCFISTEEIYHPLLEHPVSNSVQLQKPGS
                     ILTGSNMSGKSTFLRTIGVNALFAQTIYTCFAKSYRASYFSIVTSINKDDYLPGGKSY
                     FLSEAEAILAMINNRADHVASLCIIDEIFRGTHSLERIPAAIEVLRYLAKQNTLSVIA
                     THDLEVAKACIATYSLYHFSERVGEEGLEFDYLLKKGLTTSWNAIRILKHLGFPAEIV
                     DRATATVDKDIKSSS"
     gene            complement(8272..8487)
                     /locus_tag="VFC74_05245"
     CDS             complement(8272..8487)
                     /locus_tag="VFC74_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24777.1"
                     /translation="MEILKKALQIPQGRTSFFIMIGGILLAFPIVKFFGDNNFVLFFV
                     LILSVVTAESARTFGLILVKVRDSQNK"
     gene            complement(8590..8781)
                     /locus_tag="VFC74_05250"
     CDS             complement(8590..8781)
                     /locus_tag="VFC74_05250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015395237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YwbE family protein"
                     /protein_id="HZK24778.1"
                     /translation="MDGTKRSNIKIGAAVKVVQKQDQRSGKLTAGVVERILTNSATHP
                     HGIKVMLEGGIVGRVKEIE"
     gene            complement(9137..9505)
                     /locus_tag="VFC74_05255"
                     /pseudo
     CDS             complement(9137..9505)
                     /locus_tag="VFC74_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
     gene            complement(9592..10923)
                     /locus_tag="VFC74_05260"
     CDS             complement(9592..10923)
                     /locus_tag="VFC74_05260"
                     /inference="COORDINATES: protein motif:HMM:TIGR02679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02679 family protein"
                     /protein_id="HZK24779.1"
                     /translation="MNKLITEAAAFFQTEKGFARLLRGFIRTYRRLGRIGGTVKLVRM
                     TKEEKEALSGFMGKDYSRQNSATISLTDFQIALDKTKFAGLQLKDLLQAYIGEILLTR
                     SEEQELYRQRKEHFFLELQQAYPDELQQLILEHILNKGTGTRGLHQLYDTRPTLLKKR
                     LNCVLSAVKMLPTTYERLPGFASRVTKDPHGLDLNTETGRLFVLALQIIRAHQDASYR
                     LISTPSAEEITEILGEFKIIRDDLLNFVSCTGLLGLKEDGQLITMWQAALQDKAVINL
                     PLRELVKIAAFIPAHQLKTVFIVENSGVFSELLDHLQDKAAPPLICTHGQFRLAALLL
                     IDKLVQSGAVLYYSGDFDPEGLQMAERLLKRHPHHVKLWHYTVENYEACLSQVSLSQA
                     RLNKLKNIASSSLTAVKDSILSLQKAGYQEQLVPLLVADITAVLDKRIDNV"
     gene            complement(10950..15119)
                     /locus_tag="VFC74_05265"
     CDS             complement(10950..15119)
                     /locus_tag="VFC74_05265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02680 family protein"
                     /protein_id="HZK24780.1"
                     /translation="MSKDKKWTINRAGLLNFWYYDEEEFYFSDGKLLLRGDNGSGKSV
                     TMQSLIPILLDGRKSPDRLDPFGSRSRKMEDYLLGEKELVDRDERTGYLYLEYKRQGL
                     NQYITTGIGLRAKRYSSMDFWGFIIQDNRRVGHDLALYNTEYSGEDGKKVKVPLSRLE
                     LENRLGQGGRVVRGQKEYMELVNKHIFGFESVEAYEELIKLLIQLRSPKLSKDFKPTV
                     IYEILNESLPALSDEDLRPLSETIENMDQTKQQMEQLQRDRQSLKRLCRHYDRYNRFV
                     LAEKSAGFVKAARDVGKHKQQQYSLHLTDLRREQDALAEEYRALKKHDVFNMEEEKNH
                     LEKRIAGLKEQEAQKSALLRQKQEKERFLQDEINLEEEKNYSLQKELKDLLSEMSLAA
                     EECKFDGHTLAAAEFEEQYVQAEEFHFGLWRRDAAEYLHKLEHVLAVLQEEARVKEKH
                     RETDFELSEARRELDQRRLEEKKWYSLFEEEKEKLLTAIHQWLTASEEVRLTAEEVQQ
                     LISACNHLYEDYSYEAARSPLFQARQRCASKLESALVALTHQSKEKQKEKLELEQKIK
                     EWKEMKEPEPARHPDTVEARCELEKAGIPYLPFFAAVEFRPEVPADVRERLESAITQM
                     GLLDALIVPEKYAQAAINSNRIIKPDPHILAHTLAEYLYPTPPQEPQGLTAGDIDNVL
                     RSILIGENQQDVSVPIVNAEGRYRLSLLAGHAPREKQAVFIGKEARVNHRKQVIARLT
                     ENLAEVMTELQALHEKQEQLENRRQKLVEEYESFPRDTDVAEAYTNFKELKIQVNAQR
                     KEVERVNEKLRIIIDKLQRIREIVRSKAAGMPLDTNVEIYLSAVGCMKEYMTCLQKLE
                     ITYQKFFSGLRRLAQIGTNLQDVEEDVDGLKGELNVLSDQLIIENRQLNEVFKKLEEL
                     GAQEIRQEIESIEKRLRTIPDERVELTKKERDVGNAVVRLTEEIGQKELEYQAAAALA
                     AQWEKVFLAEYHLQLTPEEEFLTLQDNDALKLAIKIKQKIGHLLAESEREKVTESLNQ
                     SFYQEQGVLVEYRPNLDAILELEIPSEHFLVTSADDHIGIKVEQLARVSRRIQLMLEY
                     GGKRVSPYFVQKEMDKDIELQQIILSEKDRQLYEDIIVHSVGRLIRSRIYRAEKWVKQ
                     IDKLMADRDTSSGLTFSLKWKPRTAESEDELDTQDLVEILRRDSHLLSENDLDKVTRH
                     FRSQIERAKESMVEKGYGETLHQVIKEMLDYRRWFSFTLYYRKEGEAKKELTNHVFFT
                     FSGGEKAMAMYIPLFSAAYSRYLEARDDAPFIISLDEAFAGVDERNIRDMFDLVEKLN
                     FNYIMNSQALWGDYDTVPALSIHELIRPQNAPYVSVIRYYWNGQVRRMLRPQEVLPSE
                     RERAAEEQYAG"
     gene            complement(15116..>15541)
                     /locus_tag="VFC74_05270"
     CDS             complement(15116..>15541)
                     /locus_tag="VFC74_05270"
                     /inference="COORDINATES: protein motif:HMM:NF021198.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2398 family protein"
                     /protein_id="HZK24781.1"
                     /translation="DFQLELYRNTAFLTRTERKARLHLFPDNRAIADIALQFAAYVRK
                     QQQEQDIPLQYDGSLRLMPVDYEKWLEALKKEYGRGWSKQYREALISETARDLLAYLE
                     SWKMAAQDEESGVISLRPLLVRLVGDYFADFKQTGGDKA"
BASE COUNT         4330 a         3864 c         2983 g         4364 t
ORIGIN      
        1 ttacttaatt gattaaggag aaaaacattg gctgaattat tggaaattct aaaatcactt
       61 gccgacgaaa ccagatatca gctgttaaag ctgctcttac aacatgatta ttgtgttggt
      121 gccctagccc aaaagttaaa catctctgaa tctgccgtct cacaacatct gaaaattctt
      181 cgccagactg gcctaattaa aggcgataaa cgcggctact atacccatta ctatgtagag
      241 cgcgagctgt taaaacaagc agctaaagat ttaaatcaac tttcgcactt agtaccatgt
      301 agtactgcgt gtcaccaaac cgataaatgc agcagaaact gcgaagggag gggaaaacat
      361 gagcaagcct gattgtgaac atcccgaatt aagaccggag gacgggaaat gcagcgcaga
      421 acaaattaga aagtgtcacg gcaaagacaa aactcaccct agccacgcca aagacaacaa
      481 agaatagaca atatttgctg ttggacatga acaagataga aaatcttatt catgtcaatt
      541 tttttctaaa ggaggtagga tatttgagta ctacaatcat agaagtaaac caacttagta
      601 aacactttga tgattttaaa gctgttgacg aaatcagttt ttccgtcaac aacggcgaag
      661 tctttggttt tcttggtccc aatggtgcag gcaagacaac gaccataaat atgctcaccg
      721 gattggccag acccacctcg ggctccattc ttattgctgg tgaggatggt attaagcaca
      781 taaaaaaagt acagcagctg attggtattg tacctgatga gagcaatctg tacgatgaaa
      841 tgagtggttt agaaaacctg atcttttgtg cctcgctgta tggcctggag aaacaagaac
      901 gagaaagccg ggctaaacaa ctattagacc aatttggtct ggctaatact ggcaaaaggt
      961 cgttcaaagc ttattccaaa ggcatgaaac gtaagctaac tattgccgcc ggcatcatcc
     1021 atcaaccgca aattctcttt cttgatgaac ccactaccgg cattgatgtg gaaagtgccc
     1081 ggcaaataag aaaacttctt caagaactta accactatgg tacgaccatc tttttaacca
     1141 cacactacat agaagaagca gaaagactct gtaacagagt tggtttcatc gtcgaaggaa
     1201 aggttgtgcg tattggcggg cttagcgagt taatgcagga tgcacaacaa gataatattg
     1261 tagaatttgc tttagagagc aacagcacag atctgcaaaa ggctttagaa aaagagtttc
     1321 ccaaaactaa aatcaccaaa cttgaagatg ataaaatcag aattagttca ccggtaagga
     1381 ttgaactaat gccctttatg aactttttcg cccaagcgca ggtgccagtc tatgaagcca
     1441 aagtaatcca tccttcgcta gaagatgtct ttgtcaaaat aacaggtatc gagctgagta
     1501 aaatgaaaaa ggaaaaggag ggccgcaaaa aatgaacaaa tccattgcct tttggagtat
     1561 cctgatgaag gatatgaaaa actattatct aaagccgcct aacattagtt ggggcatcat
     1621 cttccccatc tcctggacac tgatgttttt tgttcgttcg caaagtgccg tagatatcag
     1681 aggtatcttg ccaggagtga tggcgctctc cattctgttt gggacaacct ctatgctggc
     1741 agtcactatt acttttgaac gcaaaagccg ctcttttgaa cgtctactgc tagcccccat
     1801 cagcctaaac atattaatgt tggccaaaac aacaggcgcc atcctctttg gcgttatcaa
     1861 tgcttttatc cctgttttct ttgcttcctt tctcgcagac ctatccggta tcaattgggg
     1921 agctgccgtg ctaagtgttg tactaattgc cgtgacctct actttcttag gcttgtttat
     1981 tgccgtctct gtcagcgagg tatttgaagc acaaacattc tccaactttt ttcgcttccc
     2041 catgatgttt ctctgtgggt tatttatccc cattccgaat ctaccggcag tagttagacc
     2101 actctctttt ttgctgcccc taacctatgg cgccgatatc ttaaaaacag caatcaatca
     2161 tactggcttt attgctcctt ggttaagctt cattgtactg ctggctttca ccctctttct
     2221 tttcctcatg agtacacgta atatcaaaaa gaagtggatt tattaaacat caagctgtcc
     2281 ctacctcctc tgactagtct aagttggtgc ctccagcaac aaaaatgcct cttctgtcct
     2341 gacgctgtaa tgcatagcgc acaaattcgc aggaaaaact aaagccgcta ctagaacaaa
     2401 aggaggtaag gataataata cacaagcaca cctacaggac attcatttta gggataatca
     2461 tcgtcggtat gctttctatt tctggctgcg ggttgtttac ccaaggaaac gacaaagaaa
     2521 ccttagttat cggagcaaca ccatggtctt cgacaattcc tcccactaat attgttgcta
     2581 caattttgga ggagcacctg gattatgatg tgcagattca ggaagttgat gttggcacag
     2641 tctacagcgg tctagcaaca ggcgatatag atattttcgt tgatgcctgg ctacccaata
     2701 tgcatggcaa ttacatgcag gaatatggcg atcgcattga tgaaataagt gtcagctata
     2761 cagaagggat tcttggttgg gtcgtgccca cagattctaa agtcgaatcc atcgccgaac
     2821 taaatgatta ccgtgatgag tttggcggac aaattatcgg tattgacgct ggagcaggca
     2881 tgatggagac ttcccagcaa attattgacg aatatgattt agattatgaa ttggttgaag
     2941 gtagtgagtg ggcgatgctt acagaggtat cacgccaatt cgaccgcgga aattcggtac
     3001 ttttcttagg gtggcgcccg cactggatgt ttgccgaata tgaccttaaa tttttagaag
     3061 accccaaggg cttgtggcag caggatgaag tgcgcaacct tgcaaccaaa ggattagcag
     3121 aacgagctcc tgaagcggtg gcatttttaa agaattggca gatgccaatc gatgatcttg
     3181 aagatatgat acgtcgtatg gagttagaaa acgaagatat tgatgtttta gtggctgagt
     3241 ggctgcagga aaatgaaagt ttagttcagg agatgctgcc gtaattactt aatttaatct
     3301 agctcaaaag caccgcggga acaaatgttc tcggcggtgc tttttgctaa gccattacga
     3361 ctaaacatca aataaacgag ctatttagca agtaagctaa ttaaaatctt tcttactttt
     3421 acctaaagac tgagaaatgc gatccaaaac aatagctaaa atcacaatac ttataccacc
     3481 ctcaaaacca gtacccacac ggacgcgcat aataccacgc agcacaacac tgcctaaccc
     3541 accggctccg atcatcgccg caatgacgac cattgctaat gccatcataa tggtttggtt
     3601 gatgccggcc ataatcgtag gcaaagcaag tgggaattcc acatcaacca acagctgtcg
     3661 tggtgaacta ccaaaagctt tagctgcttc aataatatca gccggcactt gacgaatacc
     3721 aagactcgtc atgcgcaccg caggcggcat ggagaaaaca accgtactta tgacggccgg
     3781 tacactcccc atgccaaaga aaatcaaggc aggaatcaag taaacaaaac tgggcatagt
     3841 ctgcatcagg tctagaactg ggcggaaaac accggcaacg gaatcatttt tcgccgctag
     3901 aattcccagt gtcaccccga caaaaacaga aatcaaagtt gctgtcaaaa tcagggctaa
     3961 agtaatcatg gtctcttccc acaagccaag gctgcccaca aaaaagaaac ccgcgaccgt
     4021 aaataaagcc aaaccccgac cattcaggcg ccaagcaagg tatgtgaaaa cggccataat
     4081 caatattgcc ggcagccaca gaataaagga ttcaatataa gtaaatgtgg taccaactag
     4141 cagattgata aaatcaaaca acgcaatcaa attcaagccc atccagtcca ctaaactttc
     4201 aatccacttc gctagtggca gctcaactag ttgtaaagaa gtaaagacat ttattaacca
     4261 ttgtccaaaa ttagctggca tcgtttttat cctcctctgc atctaccaga ccggaaagaa
     4321 ctgaaacacg tacaataatc cctagtaaac gatgcttatc atctaccaca gcaagaggga
     4381 attttgccct tgttgccatg ggaatcaaat caaaaacctg tgtatccgga gctgtggtaa
     4441 gcaaatcagt agttaatact ttttccattt ccttatgttc tttgattgcc tcaatagcac
     4501 tttcagccgt aatcagacct tttagttcac gattacgatt aactacaaac acactagaaa
     4561 tacgggcctc tgccattttg cgcagagcca cacggggacc gtctttagtg gtaattaatg
     4621 catcaggctt ttgcataatg ttttcggcag tcaagacatg agcgcggtta acaccgcgaa
     4681 taaacgcagc aacataatca ttggccgggc tggtaagaat tccttctgcc gtatctaact
     4741 ggacaatttc accgtctttc atgacagcaa tccggtcacc caatttcaaa gcttcatcaa
     4801 gatcgtgcgt aataaaaaca attgttttct gcaaacgggc ctgcaaatcc attaactctt
     4861 cctgcatgtc acgtcgaatg agtggatcca aagcactgaa cggctcatcc attaaaagaa
     4921 tatcaggttc aatagctaga gcacgagata aaccaactct ctgctgcatc cctccactta
     4981 actgatcagg atatttatct ttccagacac ccagatcaac tgtttctagt acttccaacg
     5041 ctcttttccg gcgttcgtca ggagcaacac cttgcagctt aagaccatat tccacattct
     5101 ccaagactgt acgatgagga aatagggcga aacgctgaaa aaccatgcca aatttttgac
     5161 ggcgcagttc acgcaattgg gtgtcgttca aacttgttaa atcctgacga tcaaccaaca
     5221 cactaccctg tgtaggttca atcaagcggt taaggcagcg caaaagggtt gactttccac
     5281 taccagaaag ccccataacc acaaaaaact caccttcatt aacagtaaag gaaacatctc
     5341 tcagtgcaac agtcaatccc attttttttt gcacttcatc ttttgacaga ccttgctcta
     5401 aggccgagac cgccctttgt gggtcagagc caaaaacctt gtataaattc tttacctgta
     5461 acttttccat cgcgttttat cctccatttt gcagctagca agattaccac cgacagtttt
     5521 aaatttccct aaataccaca aaataaccac ttgggaagtt tagactactg agtccacgta
     5581 aaaaaagaag agcaaacaaa actgaatttg ttcactcttc tcgttcatct ttatggggtc
     5641 actctattga gacaatggtt actttttcct cctaaccatg aatatccctt gtctgacaaa
     5701 acgcttccca gtaccacttc taagctattt aaactttgcc gccgtgccgt acgccataac
     5761 ttctgccgcc ccgctcataa tcgctgtcga cgcgtagcgg atgttgacaa cagcgtctgc
     5821 ccccaatgct gccgcatcct ccatcattct tttcgtagcc aaagcccgag cctcattcat
     5881 cattttatta tattctttta actccccgcc cacgagatgt ttaaaaaact gggtgatatc
     5941 acgaccagca tttctggttt ggatagtact gcctttcacc aagcccagca tttccaaatc
     6001 cttgcccgct atgtaatcag tatttactaa gatcatcgtc ttcaccgctc cttatttctt
     6061 taattctttc aattaacaca tagatactaa cgctaattag cgccaaggga atggcaattg
     6121 ctaagatcct cgccattagc ggcagaggct gcagcaaaga catacgtgag ataaatgcaa
     6181 aatacagcac cactattagt gtaacaatca caggagcaat gatctttttt gcctgcttgc
     6241 ccatcaccat ttcatctcct ctcaactaac aaaacaatcc tattattaca atactatgct
     6301 gcgtaacaga catattgctt tttacggcca ttgtctctcg cttttcactt tctctcacct
     6361 ctcgttttac ttcactttca ttttaagcaa ctcatctaaa caattataaa acattttcta
     6421 aacaagaaca aaaacctagg gcaactgact aacatcagct gcccctcttt taggttacat
     6481 tttttatctg aattaactac ttgattttat atctttatca acagtggcag tggcccgatc
     6541 cacaatctct gcaggaaaac ctaaatgctt taaaattcga atggcattcc aagaagttgt
     6601 taatcctttt ttaaggagat aatcaaactc cagcccttct tctcccaccc gttcactaaa
     6661 gtgatacaaa gagtaggtag ctatacaggc tttagccacc tctaaatcat gcgtagcaat
     6721 aacacttaag gtgttctgtt tcgctaaata gcgcaaaact tcaatggcgg ctggaattct
     6781 ttctaaagaa tgagtgccgc gaaagatctc atcaataatg cataaggaag caacgtgatc
     6841 tgctctattg ttaatcatcg cgaggatcgc ttccgcttca cttaaaaagt aacttttgcc
     6901 acctggcaaa taatcatctt tattgatgga agtgacgata gagaaataac ttgcgcgata
     6961 acttttggca aaacaagtat agattgtttg cgcaaataaa gcattgacgc caatagtacg
     7021 caagaaagtt gatttaccag acatattgga accagtcaag atagaacccg gtttttgaag
     7081 ctgtacagag ttcgacaccg gatgctcgag taacggatga taaatttcct cggtactaat
     7141 aaaacactgc tccgtgacta actctggttc cacccacgct gcctgtccag cacgcagaga
     7201 agtaactgcc agcagggcat ccagttctcc caccagcgaa tatagctgtt gtaagtgctc
     7261 tcttttacgt gaaattatca ccattgaggc ttcaaatcta cgtaattcca acaaaaagag
     7321 atagttcacg taatcgtaga gaccgaaagc gtctagtaat cttaaaatgt tatgatgtgt
     7381 tttgcgcagt aaccctttac actgctgcac tccagctgct agctctgcag catagtccgg
     7441 caaactctct gccgttaact gtgccaagtc acctgctgcc aagatgaaag agttcatata
     7501 acgaattgct ggcagactaa tcatcattct ggcggatttc ttatgtgtat ataagaagtt
     7561 tatagcaaac atcggaaaaa tcccgtaaaa gactccttta aaaccaatga taaaaggaga
     7621 taccacagcg actaatgcca agcaggccaa tactgttggc agataaccta aactagccgg
     7681 ttgcatctct tcatcccaaa ttaaattagt aacagtatca gcatcctgtc tatttaactg
     7741 ccgcagccgg atacgcagtt ttttccgcag cggtgagttc gtatcaaaca aacgaatcat
     7801 ctcgccacgc tgcgccagcc tctcggtatc aaaaagcggc ctctgcagca tattgtaaag
     7861 aacttgggcg cccggcgttg ttaaagtgcg atcaactaaa ctgtaaagta aatcaatgtg
     7921 taaatcttct ttggtttgcg gagaaatgat cgtttctgtc tgcgtctgga caggaaataa
     7981 tgtggacatt ctcgttagat cacgaaaatc cctgtccctt ttgctagctt taccccacat
     8041 cgctgccaac tcttccacga tggcggcata acgcattttg cctagcaaat accaaatcag
     8101 agcacccccg ggcagcagca aaactagacc atagtaaaaa actgtaccct gcagatacgc
     8161 cgcatagagt acagcaaata aatagcacgc taaaacaaag gatgtaaaat aaatataccg
     8221 acggtaacgt ttactcattt atttaaagaa ctcctgtctt cgatgaatct tttatttatt
     8281 ttggctatct ctaaccttaa ccaggataag tccaaaagtc cttgccgact ctgctgtaac
     8341 cacagagagt atcaatacaa aaaagagaac aaagttgttg tcaccaaaaa attttacaat
     8401 aggaaaggcc aacaggatac cccctatcat aataaaaaaa cttgttctgc cctgtggtat
     8461 ttgcagagct ttctttagta tttccatttt aaccaaccct ctcatcagat agtaaaacag
     8521 caaatttgca atactaatga caatattaag tcaattgtac cacaaataac tgcaattgtc
     8581 ccaccctcat cactctattt cttttacccg tcccacaata ccgccttcca gcattacttt
     8641 aatgccatgg ggatgtgtgg ccgaattagt caaaatccgt tcaacgacgc cagctgtaag
     8701 ttttccacta cgctgatcct gcttttgcac gaccttaact gccgcaccaa ttttaatatt
     8761 acttcttttt gtaccgtcca ttgttaatcc tccacttcac agcttattct tctacccgct
     8821 ccaaaatgat acgcttacta aaagcaccac gtgccgcagc tttgttgtca gccagtttaa
     8881 ctacttccgc ccagttaaca cccaaattag cagcaatgcc tttcagcact tccaagatat
     8941 ctgccaattc ctcgacctcc gccgcagcag aaaactcccg tacttcctcc cacaacttct
     9001 cacgcagcgc cagcgtatac tcttcaccct cggcaatatg gcaaactgcc accttaccct
     9061 cctgctcaat aatttccggt attctatccc gcactaactt atgatagaca tgctcttccc
     9121 tctcctcttc taaagggtag ggcaccacat tcttcttcac ttttctaata ccgccacgcg
     9181 gattctttac atcaccaaca aagcgtggaa tcacatggac atgaaaatga aaaacagact
     9241 gcccagccgc ctctcctaca ttagcaccca cattatagcc atccgggtga tactgctctt
     9301 ccagcagatc ttttacctta aaaattaact cattaacagc agccagctct gccggagtaa
     9361 cctcaaaata ggtggccaca tggcgcttgg gcaccaccag tgtatgtcct tcattaacag
     9421 gaaaattatc ataaaaggcc ttagccaaat cattctcgca aataatagcc ttttcagcga
     9481 tctgacaaaa gatacaacgt tccatgcagc tcttcctttc tcaccatctt tcatagtttg
     9541 ccatataggt cgataaaata tcttaaacta aaacatgtct accggtgcta attaaacgtt
     9601 atctatcctc ttgtctaata ctgctgtaat atctgctact aatagtggca ccagctgctc
     9661 ttggtaacca gccttttgta atgataagat gctatccttt acagcggtta aagatgaact
     9721 cgcaatgttt tttagtttat taagccttgc ctgagacaaa gacacttgcg aaaggcaagc
     9781 ttcataattt tcgaccgtat aatgccacag tttgacatgg tgcgggtgcc gcttgaggag
     9841 ccgctctgcc atttgcagac cttcaggatc aaagtcacct gaataataca aaacggcgcc
     9901 gctttgcacc aatttatcga tcagcagcag cgcagccagc ctaaattgac catgggtgca
     9961 gataagtggc ggcgctgctt tatcttgcaa atggtcgagt agctctgaaa atacacccga
    10021 gttttctaca ataaataccg tcttaagctg atgtgcagga ataaaggcag caatttttac
    10081 tagctctcgt aggggcagat tgataacggc tttatcctgc agagctgcct gccacatagt
    10141 aattaattgt ccgtcctctt ttaggcccag caagccagta cagctgacaa aattcagcag
    10201 gtcatcccga ataattttaa attcccctaa aatttccgta atctcttccg cagaaggtgt
    10261 agaaattagg cggtagctgg cgtcttgatg cgcgcgaata atctgtaggg ctaaaacaaa
    10321 tagacgacct gtttctgtat ttaaatctaa accatggggg tccttcgtta cccgactggc
    10381 aaaaccgggt agtcgttcat aagttgtcgg cagcattttt acagctgaca aaacacaatt
    10441 aagccgcttt ttcaatagag ttggccgagt gtcatacaac tgatgaaggc cccttgtacc
    10501 agtcccttta ttgagaatgt gctcaagtat caactgctgt agttcatcgg gataagcttg
    10561 ctgcaattca agaaaaaaat gttccttccg ttgccgatag agttcttgtt cctctgaacg
    10621 cgttagtaat atctccccta tataggcctg aagcaaatct tttagctgca ggccggcgaa
    10681 ctttgttttg tccagcgcaa tttgaaaatc agtcagggaa atggtggcgg agttctgccg
    10741 actgtaatcc ttacccatga agccggacaa ggcttctttt tcttcttttg tcatccttac
    10801 cagtttaacc gtgccgccaa tacggcctaa acgccggtaa gttcggataa agccacgcaa
    10861 aagccgggca aagccttttt ctgtctgaaa aaaagctgcc gcttcagtaa tcagtttatt
    10921 catttccctc accctttata agtcaaaagc taccctgcat attgctcctc agcagcccgt
    10981 tccctttcac tgggcagtac ttcctgcggc ctcagcatcc gccgcacctg accattccaa
    11041 taataacgaa tcactgaaac atagggagca ttttgcgggc gaattaactc atgaatggaa
    11101 agtgcaggaa ctgtatcata atccccccac agggcttgtg agttcataat gtaattaaag
    11161 ttaagtttct ctaccaagtc aaacatgtca cggatgtttc gttcatcgac gccggcaaat
    11221 gcctcatcca gagatattat aaatggggca tcatcacgcg cctcaaggta gcgcgagtac
    11281 gccgctgaga aaagaggaat gtacattgcc atggcctttt caccaccact gaaagtaaag
    11341 aagacatgat tggtcagttc ctttttcgcc tcgccttcct tgcgatagta aagagtaaag
    11401 gaaaaccagc gacgataatc cagcatttcc ttaatgacct gatgcagcgt ttcgccatac
    11461 cctttttcca ccatcgattc tttggctcgt tcgatttgcg agcggaaatg cctggttacc
    11521 ttatccaaat cattttcact gagcagatgg gagtcccgcc gcaggatctc tactaagtcc
    11581 tgtgtgtcca gctcatcttc tgattctgcc gttcttggct tccattttaa ggagaaggtt
    11641 aagccgctgg atgtatcccg atcggccatt aacttgtcaa tttgcttcac ccatttttcc
    11701 gctcggtaaa tccgggaacg aatcagacgc ccgacactat ggacaataat atcctcataa
    11761 agctgtctgt ccttctcact taagatgatc tgctgcagct cgatatcctt atccatctct
    11821 ttttgcacaa aataggggct tactcttttt ccaccgtatt ccagcatcag ctgtattctg
    11881 cgggagacac gagccaactg ctctactttt atacctatat ggtcatccgc tgaggtaaca
    11941 agaaaatgtt ctgacggaat ttccaactct aaaatagcat caagatttgg cctgtattcc
    12001 actaaaacac cttgttcctg gtaaaaagac tgatttaagc tttccgtcac tttctcccgc
    12061 tcagattctg ccaagagatg acctattttt tgcttaattt ttatcgctag cttcaacgcg
    12121 tcattatcct gcagcgtcaa aaattcttct tccggagtaa gctgtaaatg gtattcggct
    12181 aagaaaactt tttcccattg tgctgccagc gctgctgcgg cttggtactc tagctctttt
    12241 tgcccaattt cttctgtcag cctgacaaca gcattaccta catcacgttc tttttttgtc
    12301 aactccactc tctcatcagg aattgtccgc aagcgttttt ctatgctttc gatttcctgc
    12361 cggatctcct gcgcgccgag ctcctcaagc tttttaaaaa cctcatttag ctgcctgttt
    12421 tcaataatca actgatcgct aaggacatta agctcacctt taagcccgtc cacatcctct
    12481 tccacgtcct gcaaatttgt gccaatttgt gctagtcgcc tgagaccgga aaagaacttc
    12541 tggtaggtga tctctagctt ctgcaggcaa gtcatatatt ctttcataca gcccactgca
    12601 ctcagataaa tttcaacatt cgtatccagc ggcatccccg ctgctttgct gcgcactatt
    12661 tctctgattc tctgcagctt atcaatgata atcctcagtt tttcgttaac ccgctctacc
    12721 tctttgcgct gggcgtttac ttgtattttt agttctttga aattcgtata ggcctcagca
    12781 acatcggtgt cccggggaaa cgattcatat tcttctacca acttctgcct gcggttttct
    12841 agttgctcct gtttttcatg taaggcctgc agttctgtca ttacctcggc caaattttcc
    12901 gttagcctgg caataacttg tttccgatgg ttcacacgag cctctttgcc gatgaaaacc
    12961 gcctgttttt ccctcggagc atgacctgca agcaaagaca gccgatatct gccctctgcg
    13021 ttgactatcg gtacggatac atcttgttga ttttcaccga tcagtatact tcttaaaacg
    13081 ttatcaatat ctcccgccgt caggccctga ggctcctgtg gtggtgtagg atagagatat
    13141 tcagccagtg tatgagcaag aatatgggga tcaggtttaa tgatgcggtt gctgttgata
    13201 gctgcttgtg cgtatttttc cggtacgatt agcgcatcta acaagcccat ttgcgtgatt
    13261 gcactttcca gccgctcccg cacatctgcg ggcacttccg gcctaaattc caccgcagca
    13321 aaaaacggca gatagggtat accggccttc tccagctcac accgcgcctc tacggtatcc
    13381 ggatgtcttg cgggctccgg ctctttcatt tctttccatt ctttaatttt ttgctcaagc
    13441 tctagctttt ctttttgctt ctctttactt tggtgtgtaa gcgctaccaa ggcagattca
    13501 agcttgctcg cacagcgctg tctggcctga aacaagggac tgcgggctgc ttcataacta
    13561 tagtcttcat aaagatgatt gcaggcgctt attagctgct gcacttcctc agcggttaaa
    13621 cgaacctcct cacttgccgt taaccattgg tgtatcgcgg tcagcagctt ttccttttct
    13681 tcctcaaaaa ggctgtacca ctttttttct tccagtcggc gctggtctaa ttccctgcgc
    13741 gcttctgaca gctcaaaatc cgtttcccga tgcttttctt ttactctggc ctcttcctgc
    13801 agtaccgcta aaacatgctc aagtttgtgc agatactcag cggcatcacg tcgccacagg
    13861 ccaaagtgaa attcttctgc ctggacatat tgttcttcaa actctgctgc tgccagtgta
    13921 tgcccgtcaa atttgcactc ttcggccgcc agcgacatct cggacaaaag atctttcagc
    13981 tccttctgca gagaataatt tttttcttct tccagattta tttcatcctg caggaagcgt
    14041 tctttttctt gcttttgcct aagcagcgca cttttctgag cttcttgctc tttaaggcca
    14101 gcaatccgtt tttctagatg gtttttttcc tcttccatat taaaaacatc atgttttttc
    14161 agcgccctgt attcctcagc cagggcatct tgttcccgcc gcagatcagt taagtgcagg
    14221 ctatattgct gctgcttgtg cttaccaaca tctcttgctg ctttgacaaa acctgctgat
    14281 ttttctgcca ggacaaagcg attatagcgg tcataatgac ggcacagtct ttttagggac
    14341 tgcctgtctc gctgcagctg ctccatctgc tgtttggtct gatccatatt ttcaattgtt
    14401 tcagacaggg gccgcaagtc ctcatccgat aaggctggca gcgattcatt taaaatttcg
    14461 taaataaccg ttggtttaaa atctttggac agcttggggc tgcgcagttg tatcaaaagc
    14521 ttaattagtt cctcataggc ctcaactgat tcaaaaccaa agatatgttt attgaccaac
    14581 tccatatatt ccttttgtcc ccgcactacg cggccaccct gccccagccg attctccaac
    14641 tccagccggc tgagcggcac ttttaccttt ttgccgtctt cgccgctgta ttctgtattg
    14701 taaagagcca gatcgtgacc gaccctacgg ttatcctgaa taataaagcc ccagaaatcc
    14761 atgctgctgt aacgttttgc ccgcaggccg atgcccgtcg tgatatactg gttcaacccc
    14821 tgccgtttat attccagata gaggtagccc gtccgctcat cccggtcaac cagttctttt
    14881 tcgcctaata aatagtcttc cattttcctt gaccgggaac caaagggatc taaacggtca
    14941 ggacttttgc gaccatctaa caggatgggg atcaggctct gcatcgtcac agatttcccg
    15001 gaaccattgt cgccccgcag cagcagcttg ccgtcggaga agtaaaactc ttcttcatca
    15061 tagtaccaga agtttagtaa gccggctcga ttaatagtcc atttcttgtc cttgctcatg
    15121 ccttatcacc gcctgtctgc ttaaaatccg cgaaataatc gccaacaagc ctgaccaata
    15181 ggggtcgcag tgaaataaca ccggactctt catcctgcgc cgccattttc cagctttcca
    15241 gatatgccag taagtcgcgg gctgtttcag aaatcaaggc ctctctgtat tgtttactcc
    15301 agcctctgcc gtactccttt tttaaagctt ccagccattt ttcataatca acgggcatta
    15361 agcgcagcga accgtcatac tgcagcggaa tatcttgctc ctgctgctgt tttcgtacgt
    15421 aagcagcaaa ctgcagcgca atatcagcaa tcgccctatt atcggggaaa agatgtaaac
    15481 gagccttgcg ctctgtgcgc gtcagaaagg ccgtgttacg ataaagttct aactgaaaat
    15541 c
//
LOCUS       DASRMS010000111         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_28170, whole genome shotgun sequence.
ACCESSION   DASRMS010000111 DASRMS010000000
VERSION     DASRMS010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28170"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VFC74_05275"
     CDS             <1..463
                     /locus_tag="VFC74_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24782.1"
                     /translation="FLSADKEFSAEEFRAIILAIRDCQVNGSLEPLETLPITIRRALS
                     MATMGEQILRILERAENMRARGSAGGRKTAAGAARPEQMAASSIREELREHENQAKEQ
                     QREVDRRAAVKPATYEEMLSQAAHGNSNALKELQLTEEEAKKIYAKKGGLL"
     gene            535..966
                     /locus_tag="VFC74_05280"
     CDS             535..966
                     /locus_tag="VFC74_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="structural protein"
                     /protein_id="HZK24783.1"
                     /translation="MNLKNTRGYRNNNPGNLRHGSKWQGLATTQTDAAFCQFVSMTMG
                     MRALCKLLSNYIKNGHNTIKKIISRYAPSSENNTAAYIATVSQMVSIPADRVLDGVAN
                     EGGIKSLAMVAAAIVAVENGDRVHFHYWYCYAYLVFYDYVI"
BASE COUNT          336 a          219 c          303 g          323 t
ORIGIN      
        1 ttttttgtct gccgacaaag agttcagcgc ggaggaattt cgcgcaataa tactggcaat
       61 tagagattgc caagtaaacg ggtcacttga gccgttggaa accttgccaa taaccattag
      121 acgtgccctg agtatggcga cgatgggaga gcagatactg cggatcttag agcgtgcaga
      181 gaatatgaga gctcgaggtt ctgcaggagg tcgaaagacg gcagcaggtg cagcacgtcc
      241 agaacaaatg gcagcatcct ccataaggga agaactacgc gagcacgaga accaggccaa
      301 agaacagcag cgcgaggttg acagacgcgc agctgtaaag ccagctacgt atgaagagat
      361 gctgtcacaa gcagctcatg gaaacagtaa cgcacttaaa gaattacaat taacagaaga
      421 ggaggcaaag aaaatatacg ctaagaaggg aggtttatta tgaatgaagg ttgttgttat
      481 aggttctcga gttttatact aatggtttaa taaattagca atcagttata atatatgaat
      541 ctaaagaata caagagggta tcgtaataac aatccaggta acctgcgcca tgggtctaaa
      601 tggcaaggcc tggccactac gcagacagat gcagccttct gccaattcgt ttcaatgaca
      661 atggggatgc gtgctttatg caagttacta tcaaattata tcaagaatgg acacaacaca
      721 attaaaaaga ttatatcaag gtatgcacca agtagtgaaa acaatactgc agcttatatt
      781 gctacggtta gtcaaatggt tagtatccca gccgatagag ttttggatgg cgttgctaac
      841 gaaggtggca tcaaatctct tgcaatggtg gctgcagcta tcgttgcggt tgaaaatggt
      901 gaccgcgttc attttcatta ttggtattgt tacgcttatc tggtatttta tgactatgtg
      961 atatagtaac gctgatgggc gcttagttac tttttcttta tggctttttg gtttgttggt
     1021 ccatgggggt ttatagatct ccatggacct attttttttg tgtcctggtc taatctgcag
     1081 ggtgtcctgg tctaatctgc agggtgtcct ggtctaatct gcagggtgtc ctggtctaat
     1141 ctgcagggtg tcctggtcta atctgcaggg tgtcctggtc t
//
LOCUS       DASRMS010000112        16034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_230609, whole genome shotgun sequence.
ACCESSION   DASRMS010000112 DASRMS010000000
VERSION     DASRMS010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 16034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16034
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_230609"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1429)
                     /locus_tag="VFC74_05285"
     CDS             complement(212..1429)
                     /locus_tag="VFC74_05285"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HZK24784.1"
                     /translation="MIAFEQAQKILCQEIERLLANTNNVEQLSLWQAGDRVLAQDIVA
                     ADNVPAFDRSLVDGFALLAADTEGATAEQPITLPVTATVAAGARAAKALLPKTAVRIF
                     TGALLPDGTDCVVKEEKVIVTDEAIVLKHEEDKGEGISFKGEDIALGEHLLSCGTRLA
                     PADLAILATLGIDPVPVFKQPEVGIFSTGNELVDVNCSLQPGQLRASNLYVLAELVRQ
                     AGGVPVNLGLVGDSEEEVLAVYAEAEERDLPLILSTGGTASGDYDVIKAAMDKFSGGR
                     LFNKVAMRPGAPVVVSAGSRQLLIGLSGNPAGATVAMLLLIAPLIARLAGNKHFLRRQ
                     QARLTQPIFYRSALRGFFWGNYDQQADNIYVTPHVNQFCASIKTYVQSNCLIEAADGH
                     NIERNQLVTIFPW"
     gene            complement(1484..2233)
                     /locus_tag="VFC74_05290"
     CDS             complement(1484..2233)
                     /locus_tag="VFC74_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobB"
                     /protein_id="HZK24785.1"
                     /translation="MKVFSVFGITKSGKTTTIEKIIAELRKRRYSVGSVKEIHFEKFA
                     IDTEGTNTDRHKKAGSQLVTARGMYETDVLLQRQLAVNEILSFYDHDYVLLEGVTDTC
                     APKIVTAHSTEEVDARLDETVFAISGRLANSMQEYRGLPVINAITETDKLVDLIIEKV
                     FTRLPDVAPECCAACGVSCTELSASILQGTAKREDCVISDGSVTLTIDDQELSIVPFV
                     QKLLYNAVTGVAKELDGYRGNGEIVVRIKGK"
     gene            complement(2248..2991)
                     /locus_tag="VFC74_05295"
     CDS             complement(2248..2991)
                     /locus_tag="VFC74_05295"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HZK24786.1"
                     /translation="MWQTPTLDPRTKLLIVLCLSSLAVLIKNVYILLLVLVLTLAAVK
                     LTGGEIWHVFSKLKRFISIFLAVIILQSVFTNEGQAIVQVMQVKLITDVGLLRGLQTI
                     LRFFIVIAAAVLMTTANPRDIIQGLIQWKVPYELAFMVTIAIRFLPLLQEEATDTLTA
                     LQLRGVNLKQIPILKRLRTYSYLLMPLLTSVLMKSRDLSVAMEMRAFRAYPQRTSLRE
                     LQFSGIDYLAMALSMLIFLLLLVAALGFN"
     gene            complement(2969..3634)
                     /locus_tag="VFC74_05300"
     CDS             complement(2969..3634)
                     /locus_tag="VFC74_05300"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24787.1"
                     /translation="MISLQDVSFHYKRGKWVFQELNLEIRRNLITFIVGPNGGGKTTL
                     GKLMAGILKPQTGMVLIDNLNTKEVPLAELGAKVGYLFQEPERQIFANTVREEIGFVL
                     DLREVPPAESKAKVDQALAQFQLTHLAALSPLKLSRGEKQRLVIAAIMVNQPQFFILD
                     EPTTGLDQERKAILSGVIEGLQQQGIGMAIISHDQSFVQRHAQQLITVAKGEIVDVAN
                     PNT"
     gene            complement(3656..4345)
                     /locus_tag="VFC74_05305"
     CDS             complement(3656..4345)
                     /locus_tag="VFC74_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24788.1"
                     /translation="MSLPILVKDLSFQYENDATQILRHLNLAVAKGEIVAVVGLSGIG
                     KSTLCYCLSGLIPHVYGGMMEGEVLLQGQNTKELSLAAIATTLGIVFQNPDHQLFSFN
                     VEDELAFGPENLCVPRAEIARRIEEVLAKVGMQDYRDASPQQLSGGQRQLIAMAAVLT
                     LQPEILIFDEAMSQIDADGKAMLKAMMLRLKEEGKTMVMIEHDLDNLQIADRVLVLRE
                     GQLSPYTGSLS"
     gene            complement(4342..4848)
                     /locus_tag="VFC74_05310"
     CDS             complement(4342..4848)
                     /locus_tag="VFC74_05310"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK24789.1"
                     /translation="MKKVVIVLVILVVIVAITAMLNRDDLGEKVASQENALLYLKADN
                     SETKVDFATMQELPEVEFPAVLKRSGKAPVDTSYAGVELKALLAKLEINTDDKTQIIT
                     KAVDGYTVALSLAEVLADDNVYIVYKRDGEDLGTKEDGGSGPYQLVIREDSFGQRWNK
                     YLMEIELQ"
     gene            complement(4845..6347)
                     /locus_tag="VFC74_05315"
     CDS             complement(4845..6347)
                     /locus_tag="VFC74_05315"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HZK24790.1"
                     /translation="MPKKYLLMMLLLVIGVFLGGLLREAFVRKTAQAAVSFVPTLRIG
                     GDVNQPLTIKEPTDFAQVDFTYKGECFTGIPLAEIVRSAEPITEDSKILFIADDGLSA
                     VIDSQDLTGCHLLFSAHGWEAINLFHPVSSNIKRIQSIIIIAADAPLNYGMNVITQDA
                     NLAHFTPGQLYQLASRHLPYFEGTSTVTPEGKQNSVSIYTRRQLYPLAELVELTGEEK
                     LIVMGAHGEYLTTEESGFLEVNNNLLTYITLDRKAEIEDVRGMIINPPTASITDLYHD
                     TLHFLQQDEKVLLIYLDGLGFHQYEKALAEGYAPFLASLPAAVKATSVYQPVTNAGFA
                     AMITGTTPAENGVYSRKQMDLKTPSIFAKAQELGKKSALIEGHIKVLNTEIEPVLNLD
                     TNGNGSNDDEIFAAGQQYLAADDYDLLMVHAHSIDDAGHTNGDFHVKTMEQIKLIDSY
                     AESLVKAWRGKVIITADHGMHSTNEGGGHGEFRYEDLFVPYLVCEGGVTE"
     gene            complement(6349..6915)
                     /locus_tag="VFC74_05320"
     CDS             complement(6349..6915)
                     /locus_tag="VFC74_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HZK24791.1"
                     /translation="MLSKLLKKFTLFDMVLLALMACLGIATKPIIVPLTHIITGPLFV
                     PGGVIAGGFYMMWIVLGVGLVKKPGAATLIAIVQAIMVTATGFFGTHGLFNIFTYLLP
                     GLAVDLVMIISRHRGCCLGCCFAGGIAANLSGTLLVNLVFFRLPWVPLLLSLSSAALS
                     GGLGGIIAYTLLRQLDKSGITSVLNRRG"
     gene            complement(6926..7405)
                     /locus_tag="VFC74_05325"
     CDS             complement(6926..7405)
                     /locus_tag="VFC74_05325"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK24792.1"
                     /translation="MKKLSLLLVVLLLSVALIGCSQPEEETPTADPAWTISIVGVEAE
                     PIDFTDLDTAELSQKDVEAVKKKKDGTEITENWQGVPLKEVLAAVGAADYQSVTVEAS
                     DGYAQEYTLEIINREETILGLVRDGEKLDEADGPVQMVPAGESGNMYIKNLAKITVQ"
     gene            complement(7423..8511)
                     /locus_tag="VFC74_05330"
     CDS             complement(7423..8511)
                     /locus_tag="VFC74_05330"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HZK24793.1"
                     /translation="MKKNLFLLSLVLLLLFTAACGSNQPAPGGGEDTPQGEEAIVIAG
                     SELGEHKITVAELKELTPVEKEVVSVSSSGEENEYSVKGALFADVLAELGLQQKELSA
                     IRFIAGDGYMMEVPQEVLAARDIILAYEIDGEPLQEKTKPLRAIVPEERSMYWVRNLV
                     KIEILEAAVAEPLSKLMFLETVTSTMEQQDYTYYESTDQAVLCSELPLPDAGETVYLK
                     ASDGLDKNEKAAVFLEEGYIKVTGEAKPLYLSPNLPKGMHVKEILWLAKAEVGIFSVE
                     QGLKYFTVTVKDGEEGIALTDILAEVQLAASEQYLLTAADGYEVEISKADLAQGLVYV
                     RDSGEITVIFEGLPKNTTVKSLLAIEVK"
     gene            complement(8513..9007)
                     /locus_tag="VFC74_05335"
     CDS             complement(8513..9007)
                     /locus_tag="VFC74_05335"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HZK24794.1"
                     /translation="MFVVVVWAVLPNLSQKIPAYITAGLQDTETLTRQKNLEERNIKE
                     VYLVADQTDQPAELLLIVETMGYNGPLKLRVQLDVKKSILANLTILAHEESATYGAYA
                     AEEWFLERFNGKDTAHDLRLAKVAAQNAQEVVAVTGATITSKAIIDGVNQAFSTYRKY
                     QEGN"
     regulatory      complement(9115..9232)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            complement(9324..9896)
                     /locus_tag="VFC74_05340"
     CDS             complement(9324..9896)
                     /locus_tag="VFC74_05340"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HZK24795.1"
                     /translation="MEEAKIKETKTLQDLLALSETCRACVLGKDRNKLVFGEGDPQAK
                     IFLLAEAPGAKEDELGRPFVGRAGELLTTLLAQAGIKREDVYITGSCKCRPPKNRNPY
                     KKELTACQPIIKQQLAIIRPQVVVCLGLVAVHNLLEPKVRLADVRGKWFEGSGYRIYP
                     TYHPAAVLRGTVKADILVEDFQRVSASLVS"
     gene            complement(10040..13114)
                     /locus_tag="VFC74_05345"
     CDS             complement(10040..13114)
                     /locus_tag="VFC74_05345"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZK24796.1"
                     /translation="MSFVHLRVHSEYSFLRSACRLEELVLRAKELGMPAVALTDYGVV
                     HGAVRFTQLALQHGLQPILGVELNLSSGLPVVLLVRNKTGWGNLLRLINGCQLSQPAK
                     AWLEPQELADHYEGLSLVLHPSYQSFSLNTVEKALTMLQPIFAAELYLALADTGLPGT
                     SRFNQQLAALAQTQGVSLLASQNVHYVLSGEESIFWMVNCIRTGKPLAGARQAFLPGS
                     NYHLATPAEMWRRFASFPQACDTTLFLAQKCSFVLQREGRHLPTFHLPNDEAAESALP
                     RLCREGLQRTGLRAEVALRRLERELAVILPKGLAAYFLIVQDLVNYARRAGIPVGPGR
                     GSAGGSLVAYLLGITQVDPLAHGLYFERFISEDRVEFPDIDIDVCQRRRGELLDYLRS
                     RYGEGRVAQVSTFSTLGARAAVREVGKILDASGDKVSAVAEALPHYSDQGGIEAALRQ
                     FPEFQQARLRDETAQAILVGARRLEGLCRHLSTHASGVILGLADLAEAAPLCRGPAGE
                     VLTQWDKDDVEAMGFLKLDVLGSRNLTIIHDTLASVAARHGGAPTVEKIVTDDQATFA
                     MLRRGESLGCFQLESAGMRRVLRLLVPERLEDLIHLLSLYRPGPWESGMVETFVARQR
                     GQEETTYLHPALAEILAETHGVVLYQEQVMRIACAVGGYTAGEADQLRREMGRRAPSL
                     QEVHRLKFITGAVQKGFSTKDAGDIFDHLCRFAGYGFNKAHSAAYALFSYWTAYLKQH
                     YPVEYYAALLSAGFGYYGSAVYIHEARRRGIEILPPHVNYSEITFQPEGSAIRAALSL
                     VRDLGARGAATIIDARAHEPYTSLQDFCQRVGRSALRRKALANLVKAGGLAGLGLNRR
                     QALAALDHVLKWTDRQPGQLSLWPWLTEENTLPSLPDFSAAEVITAEAETLGLSLSQH
                     PLQPYRSQLKHGHYLQLQELSFRPAGQEVTVAAAVLARSRRRLKKGGVMLTLLLSDES
                     GFAEAIFYPDVYKRLLYSLNEDLLVLTGHTTTDGDAIIVQQALPLWSYCLQN"
     gene            complement(13335..14225)
                     /locus_tag="VFC74_05350"
     CDS             complement(13335..14225)
                     /locus_tag="VFC74_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK24797.1"
                     /translation="MSLTLKQVSGKTYYIPSPVNIGVLIKANDCIVIDTGLDKGVGQQ
                     ILHCLQQAGLRVKAIINTHSHADHCGGNAPLREAGAIVCASQLEKALIENTKLEPFYL
                     FSAAPVNELKSHFLMAPASPVEQVLETGPCTMLGIDLEILDLAGHSPGQIGVLTADGV
                     CFCGDAYVGELILDKFNMSYTADVSAALQTLIKLQHSSFQYYVPAHGEVTSDTSEIIT
                     KNIAAIEKTLAQILTFIQKEPLTREEILAYYITTTKRRFDAIQYVLNFSTITACLSHL
                     HQTNQVAYSFVDGKMLWQAV"
     gene            complement(14311..14871)
                     /gene="cobO"
                     /locus_tag="VFC74_05355"
     CDS             complement(14311..14871)
                     /gene="cobO"
                     /locus_tag="VFC74_05355"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF004637.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZK24798.1"
                     /translation="MLIINNEVKQMKDRQGIVMVYTGNGKGKTTSALGLALRASGHGE
                     KIFMVQFRKSDPTYGEIQGIRKFLPNMTVLQSERSRFRKHGDFEQADYTDAANVFTQG
                     KEALLSGEYDLVIFDEINFALYNDLLELDDVLAMLKARPRHANVVLTGRYACESIIEA
                     ADLVSEVKEIKHHYQAGIKAQPGVEY"
     regulatory      complement(15046..15231)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(15362..15991)
                     /locus_tag="VFC74_05360"
     CDS             complement(15362..15991)
                     /locus_tag="VFC74_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HZK24799.1"
                     /translation="MSKLAEVKAQVEAGKTKIVPGLVQEALDEGLAAKDILAAMVDAM
                     GVVGDKFSAGEIFVPEMLIAGKAMQKGVEILKPALAGDSSISLGKCIIGTVQGDLHDI
                     GKNLVCLMIESAGFEMIDLGVDVSKETFVDTIKANPDVKVVALSALLSTTMPSMRDTV
                     AAIKESGFTGFKTIVGGAPITQEFAAEIGADAYARDAGGAAIKVKELAS"
BASE COUNT         4403 a         4053 c         3210 g         4368 t
ORIGIN      
        1 ccactcccat gtttgaccct gggatcccac aaatgtataa agagcaacct cattcttttt
       61 actatgaaac ttttcttggc agtgatagga gacaatatcc cattttttgc agaaatcccg
      121 ttcaaacatg aaaaaccttc ctttactaat tacgggtaaa accctttatc gttgactgtg
      181 acagtctaag cttactttta ctacccctgt gtcaccaggg aaagattgtc actagttggt
      241 tacgttctat attatgaccg tcagcagctt ctattaaaca gttgctctgc acataagttt
      301 taatagaagc acaaaattga ttaacatggg gtgtcacata gatattatct gcttgctggt
      361 cgtaattacc ccagaaaaat cctcgcaaag cagagcgata gaaaataggc tgtgttaatc
      421 tggcttgttg ccgtcgtaag aaatgtttgt ttccagcaag ccgggcaatc agtggtgcaa
      481 ttaacaacag catcgccact gttgcgccag caggattgcc ggacagtcca attaataact
      541 gcctggatcc ggcagaaaca acaacaggtg ccccaggtcg catcgctact ttattaaata
      601 atctgccgcc agaaaattta tccatggcgg ccttaataac atcataatca ccagaagcag
      661 tgccaccagt agacagtatc agcggcagat ctcgctcttc tgcttccgca taaactgcca
      721 gcacttcttc ctcactgtct cctaccagac ctaaatttac cggtactccg cctgcttgac
      781 gtaccaattc agctaaaaca taaagatttg aagcacgcag ctgtcctggc tgtagtgaac
      841 agttgacatc aactagctca ttacctgtag aaaagatacc cacttctggc tgtttaaaaa
      901 cgggcaccgg atcgatgcct aaggtggcga ggattgctaa atcagctggc gcaagacgtg
      961 tgccacagct taataagtgt tctccaagag caatgtcttc acctttaaaa ctgataccct
     1021 cgcccttatc ttcctcatgt tttaaaacta tggcctcgtc agttactatt actttttctt
     1081 ctttgacaac acaatccgta ccatccggca gcagggcccc tgtgaagatt ctcaccgctg
     1141 tcttaggcag taatgctttt gctgcccttg ctcccgccgc cactgttgcc gtcacaggta
     1201 aagtaatagg ttgttcggct gtcgcccctt ctgtgtctgc tgccaatagc gcaaaaccat
     1261 ccaccaaaga acggtcaaag gcagggacat tatctgcggc cacaatgtct tgtgctaata
     1321 ctctgtcgcc agcctgccat aaggacagct gctctacatt attggtgttg gctagtaaac
     1381 gttctatctc ttggcacaaa attttttgcg cctgctcaaa ggcaatcacc tggtttcact
     1441 cccactttac tgtaaatcac actggtcaac gatacttaca gttttatttt cctttaattc
     1501 tgaccacaat ttccccgttg ccacgatatc cgtctagctc tttagctaca ccggtgactg
     1561 cattgtaaag cagcttttgc acaaaaggca cgatggaaag ctcttggtca tcaatggtca
     1621 aagtgacact gccatctgat attacacagt cctcgcgttt tgctgtacct tgtaagatac
     1681 tggcggataa ttccgtacaa ctaacaccgc aggcagcaca acattcaggg gcgacgtcgg
     1741 gcaagcgtgt aaatactttt tcgattatca agtcaaccag cttgtccgtt tcggtgatag
     1801 cattaatgac cggcaaacca cggtactcct gcatactatt agccagcctg ccagaaatag
     1861 caaagactgt ctcatccaga cgtgcatcga cttcctctgt gctgtgagca gtgacaattt
     1921 tcggcgcaca ggtgtcggtc acaccttcca gcaacacgta atcgtgatcg tagaaactta
     1981 atatttcgtt cacggctaac tgccgctgca gtagcacatc tgtttcatac ataccgcgtg
     2041 ccgttaccag ctgcgagccg gcttttttat gtctgtcagt attggtgcct tcggtatcaa
     2101 tagcaaactt ttcaaaatga atttccttga ccgagcctac actatagcgt ctttttctca
     2161 attcagcaat aatcttttct atcgttgtag ttttccccga ttttgtaata ccaaatactg
     2221 aaaaaacttt cacgctgtct acacctccta attaaagccc aaagctgcca caagcagcag
     2281 tagaaaaata agcatactta atgccattgc taaatagtca attccgctga actgcaattc
     2341 ccgcaaactc gttctttggg gatatgcccg aaaagcgcgc atttccatag ccacagacag
     2401 atccctggat ttcattaaaa cacttgtcaa caggggcatt aacagataag aataagtacg
     2461 cagccgcttt aaaatgggga tttgcttgag gttgacgccc cgcagctgca gagcagtaag
     2521 cgtatccgtg gcttcctcct gcaggagggg taagaatcta atggcgatgg ttaccataaa
     2581 ggccagttcg taaggcactt tccattggat aagtccttga ataatatcac gcgggttggc
     2641 agttgtcatc agcacagccg cggcaatcac gatgaaaaaa cgcaaaattg tctgcaggcc
     2701 ccgcagtaat cccacatcag taatcagctt aacttgcatt acttgcacga tggcctgacc
     2761 ctcgttagta aaaacacttt gcaggataat tactgccaaa aaaatagaaa tgaatctctt
     2821 gagcttactg aacacatgcc agatctcacc gccggttaac ttcaccgcag ctaaagtcag
     2881 tacaagaacc aacagcaaaa tatagacatt tttgatcaag acagccaggc tggaaaggca
     2941 aagcacaatc agcagttttg ttctcggatc aagtgttggg gtttgccaca tcaacgattt
     3001 ccccctttgc cacggtgatc agctgttggg catgtctttg tacaaaactc tggtcatgac
     3061 taataatggc catgccaatc ccctgctgct gcaacccttc aatcacgccg gaaaggatgg
     3121 cttttctttc ttggtccagt ccggtagtcg gttcatcaag aataaaaaat tgcggttgat
     3181 taaccatgat ggcagcaatc actaaacgct gcttttcgcc acggcttaat ttaagcggcg
     3241 acagtgccgc taaatgagtt aattgaaact gtgctagggc ctgatcaact ttagctttgc
     3301 tctcagctgg cggcacttca cgtaagtcca aaacaaagcc aatttcctcc cgcactgtat
     3361 tcgcaaagat ttgccgctct ggctcctgga aaagatagcc cactttagct ccaagttcag
     3421 caagcggcac ctctttggtg tttaaattat ctatcagcac catccctgtc tgcggtttaa
     3481 gaatgccagc catcagctta cccaaggttg ttttgccgcc gccattggga ccgacaataa
     3541 aagttatgag atttcgccta atctctaaat tcagctcttg aaatacccat ttacctcttt
     3601 tgtagtgaaa actcacatct tgcaagctga tcattatgct catcctctca cctgcctatg
     3661 acaggctgcc agtgtaggga gacagctggc cttcccgcaa gaccaatacc cgatcggcaa
     3721 tctgtagatt atctaggtca tgttcaatca tcaccatggt tttaccttct tcttttagac
     3781 gcagcatcat agctttaagc attgctttcc cgtcagcatc aatctgcgac atggcttcat
     3841 caaagataag aatctcgggc tgcagcgtta atactgcggc catggcaatc agctgccttt
     3901 ggccgccgga aagctgctgg ggactggcat cgcggtaatc ctgcatgccc acctttgcca
     3961 gtacttcttc aatgcgcctg gcaatttcag ctcggggcac acaaagattt tccgggccga
     4021 aggccagttc atcctcaacg ttaaaagaaa agagttgatg atccgggttt tgaaaaacaa
     4081 tacctaaagt ggtggcaata gcagctaaac ttaactcttt ggtgttctga ccttgcagca
     4141 gcacttcgcc ctccatcatg cctccgtaga catggggaat taggccactc aaacaatagc
     4201 agagggtgct tttaccaatg ccgcttaaac ctactacggc cacgatctca cctttagcta
     4261 cagctaagtt taaatgacgc aaaatttgtg ttgcatcatt ttcatactga aaggataaat
     4321 ctttgactag aattggtaaa ctcactgtag ttcaatctcc attaagtatt tattccatct
     4381 ttgtccaaag gaatcttctc tgatcaccag ctgatagggg cctgagccgc cgtcctcttt
     4441 ggttcccagg tcctcgccat cacgcttgta gacaatataa acattatcat ctgccaacac
     4501 ttccgctaga gacagggcta cggtataacc atccacagct ttagtaataa tttgagtttt
     4561 atcgtctgtg ttaatctcca gcttcgctag cagtgctttt aactctacgc cggcatagga
     4621 tgtatctacg ggtgccttac cgcttctttt taaaacagca ggaaactcta cttcaggcaa
     4681 ttcttgcatg gtggcaaaat caacctttgt ttcgctgtta tctgctttaa gatacaataa
     4741 ggcattttcc tgactggcca ctttttcccc taaatcatca cgattaagca ttgctgtaat
     4801 ggcaacaatg accacgagga taaccaggac gatcacaact tttttcactc ggttactcct
     4861 ccctcacaca caagataggg cacaaataaa tcttcatagc ggaattcacc atggcctccc
     4921 ccttcgtttg tcgagtgcat tccatgatcg gcagtaatta ttaccttgcc gcgccatgct
     4981 ttcaccaagc tctctgcata gctatcaatt agcttaatct gctccatagt tttcacatgg
     5041 aaatcaccat tggtatgccc ggcatcatcg atactgtgcg catgtaccat cagtaaatca
     5101 taatcatcag cagccaggta ctgctgacca gcagcgaaga tctcatcgtc attactgccg
     5161 ttaccattag tatcaagatt taaaacaggc tcaatttctg tattgagaac ttttatatga
     5221 ccttcaatta gggcagattt tttgcctaac tcttgtgctt tagcaaaaat agaaggagtc
     5281 tttaaatcca tttgcttcct cgaataaact ccattttctg ctggtgttgt ccctgtaatc
     5341 atagcagcaa atccagcatt agtcactggt tgatacaccg atgtggcttt caccgctgca
     5401 ggcaagctgg caagaaaagg cgcatagccc tcagccagag ccttttcata ctgatggaag
     5461 ccaagaccat cgagatagat taagagcact ttttcatcct gctgaagaaa atgcaaggta
     5521 tcatgatata aatcagtaat gctggctgtg ggtggattga tgatcatacc cctgacatct
     5581 tctatctcag ctttacgatc taatgtaatg taggtaagca gattattgtt cacttccaga
     5641 aaacctgatt cttcagtagt taagtactct ccgtgggcac ccatgacgat taacttctct
     5701 tctccagtca gttctaccaa ctcagcaaga ggatataact gccgccttgt atagatggag
     5761 acactatttt gcttaccctc aggcgtgacg gttgaggtgc cttcaaaata aggaagatgc
     5821 ctacttgcca gctgataaag ctggcctggc gtaaaatggg ccagattggc gtcttgagta
     5881 atcacattca tgccataatt taagggcgcg tctgccgcaa taataataat tgactgaata
     5941 cgcttaatat tactgctgac aggatggaaa agattaatgg cctcccagcc atgagcagaa
     6001 aacaggagat gacaacctgt taaatcctga ctatctatta ccgcgctcaa accgtcatca
     6061 gcaataaata atatcttact atcttcggtg ataggctctg cacttcgtac tatctctgca
     6121 aggggaatgc ctgtaaaaca ttcccctttg taagtaaaat caacctgagc aaaatctgtg
     6181 ggctccttga tcgtcagcgg ctgattcacg tcaccgccaa tccgcagagt aggtacaaag
     6241 ctaacagcag cctgggcagt ctttctgacg aaagcttcac gcaaaagacc accaagaaag
     6301 acgccgatca ctagcaaaag catcattaac agatactttt tcggcatact atcccctcct
     6361 gtttagtaca ctagttatcc cgcttttatc taattgcctg agcagagtat aagcaataat
     6421 tcctcccaag cccccggaaa gagccgcact ggagagactt aacaatagag gaacccaggg
     6481 caggcgaaaa aatactagat ttactagtaa ggtaccgcta agattagccg caatgccgcc
     6541 ggcaaaacag cagccgaggc agcagccccg atggcgggag ataatcataa ccaaatctac
     6601 tgccaggcct ggcagtagat aagtaaaaat gttaaataac ccatgggtgc caaaaaaacc
     6661 agtagcagta accatgatcg cctgcacaat agcaatcagt gtagcggcgc cgggcttctt
     6721 aactaaaccc actcccagga caatccacat catgtaaaag ccgccggcaa tcacgccgcc
     6781 cggcacaaaa agcggaccgg taataatatg tgtcaaggga acaataatcg gttttgtggc
     6841 aatgcctagg cacgccatta aagccagcag taccatgtca aaaagtgtaa atttctttaa
     6901 aagcttactt aacatgtttg atcacttact gtacagtaat ttttgccaga tttttaatat
     6961 acatattgcc tgattcaccg gctggtacca tttgtaccgg accatcagct tcatctagct
     7021 tctcgccgtc acgcaccaag cctaaaatag tttcttcacg gttaatgatc tctaaagtat
     7081 actcttgcgc ataaccatcg ctagcctcta ctgttacact ttgatagtca gctgcaccga
     7141 cagcagccaa aacttctttt agtggtacac cctgccagtt ttctgttatt tctgtgccgt
     7201 cttttttctt tttcacagct tccacatctt tttggcttaa ctctgctgta tccaagtcag
     7261 taaaatcaat cggttcagct tccacaccga caatactaat agtccaagcc ggatcagctg
     7321 tcggagtttc ttcttcaggt tgtgaacaac ctattaacgc aacacttaac aacaacacaa
     7381 cgagtaataa agataacttt ttcatttttt cgccccctga ttttatttta cttctatggc
     7441 tagtaaactt ttaacagtag tatttttggg cagaccctca aatattactg tgatttcacc
     7501 actgtctcga acatacacca gcccttgggc cagatctgct ttgctgattt ccacttcata
     7561 accatcggct gccgttaaca gatactgctc actagctgcc aattgtactt cagctaaaat
     7621 atctgttaaa gcaattcctt cttctccatc cttaacggtc acagtaaaat attttagccc
     7681 ttgttctaca gaaaagatgc caacttcagc tttagctagc cagagaatct ctttgacatg
     7741 catcccctta ggcagattgg gtgagagata aagtggttta gcctcaccgg taactttgat
     7801 ataaccttcc tccaagaaca ctgccgcttt ctcattctta tccagaccat cactggcttt
     7861 caaatagaca gtctcaccag cgtcaggcag cggcagctca ctgcataata cggcctgatc
     7921 tgtgctttca taataagtat aatcctgctg ttccatggta ctggtcacag tttccaagaa
     7981 cattagttta ctaagaggtt cagctactgc tgcctcaaga atttctatct ttaccagatt
     8041 tcgcacccag tacatggaac gctcttcggg aacaatggct ctaagcggct ttgttttttc
     8101 ttgtaagggt tcaccgtcta tttcataggc caaaatgata tcccgcgctg ccagaacttc
     8161 ttgaggaacc tccatcatat aaccatcgcc ggcgataaaa cgaatggcac ttaattcttt
     8221 ttgctgcagc cccaattccg ctaagacgtc cgcaaataag gcacctttaa cactgtactc
     8281 attttcctca ccagatgaac tgacagagac tacctctttt tctacgggtg ttaattcttt
     8341 taactcagcc acagtaatct tgtgctcgcc tagctctgaa ccagcaatga caatggcttc
     8401 ttccccctgc ggcgtgtctt ccccaccgcc aggtgcaggt tgattgctgc cacaagccgc
     8461 agtaaacaac agcagcaaga ctaaagacag caaaaacaag ttctttttca ccttaattcc
     8521 cctcctgata ttttctataa gtgctaaaag cctgattaac accatctata atcgccttgc
     8581 tggtaatagt ggctccggtt acagctacta cttcctgcgc attctgagcc gctacttttg
     8641 ccaagcgtaa atcatgagct gtgtctttgc cgttaaagcg ttctaaaaac cactcttctg
     8701 ctgcatatgc accgtaagta gcactttcct cgtgcgctaa aatcgtgagg tttgcaagta
     8761 tacttttctt gacatccagc tgcacacgaa gtttaagagg accgttatag cccatagttt
     8821 ccacaatcag cagcagctcg gcaggttgat cggtttggtc tgcaactaaa taaacttctt
     8881 taatattcct ttcttctaaa tttttttgcc tagtgagagt ctcggtatcc tgtagacctg
     8941 ctgtaatgta tgctgggatc ttttgactta ggttaggcaa aacagcccaa accacaacaa
     9001 cgaacacaac tgtagctaca aaccaacgat attttgtctt aattaggcac acccccgagg
     9061 tagtgaaatc aaaaaacctc ttcccaaggg aagaggtaaa aataggcaag tcaaaataaa
     9121 cttgattgtt ctcctttcca ctgggaggct gtaccgtttc ctgtacaacc ctaaacggtt
     9181 tatgctcata ggtttcccct tagatgacat aaactcggag tcactattct tttattgtaa
     9241 cctagctgca ggctgttaaa agcactacat ttctatactt tacattatag ttttagggct
     9301 actttgtcaa ctatcattta cccttatgac accaagctag cagacacgcg ctggaaatcc
     9361 tctactaaaa tgtctgcttt aactgtgccc cgcaaaactg ctgccggatg ataggtggga
     9421 tagatcctat agccactgcc ctcaaaccac ttaccgcgca catcagcaag ccgtactttg
     9481 ggctccagta agttatgcac agctaccagt cccaaacaga ctaccacttg agggcggatg
     9541 atagccaatt gttgtttgat gatcggctga caagcagtta gttctttttt atatggatta
     9601 cgattcttgg gagggcggca tttacatgat cccgtaatat aaacatcctc gcgcttaatg
     9661 ccggcttgag ccaacagtgt tgttagcagt tctccggcac gtcccacaaa tggtctgcca
     9721 agctcgtctt ctttggctcc cggagcttcg gccagaagaa aaattttcgc ctgcgggtca
     9781 ccttctccga aaacaagttt gttacgatct ttgcctagca cacaggctcg acaggtttca
     9841 cttaatgcca ataaatcctg cagcgttttt gtttctttga tttttgcttc ttccactact
     9901 gctaactcct ttgttttgga cttgctaagt tatttcgaca tcaggcttta ttctcctgtt
     9961 aaaaaagcaa gcgcttttca attttagctt gcctgctttt tataccaatt tcattagccg
    10021 cttgctaaga ggaaatgagc taattttgta ggcagtaaga ccacaaaggc agtgcctgct
    10081 gcacaataat ggcatcaccg tcggtggtcg tatgtcctgt taacaccagc aaatcttcat
    10141 tcaggctgta tagtaggcgt ttataaacat ctgggtaaaa gatagcctcc gcaaaaccgc
    10201 tttcgtctga caaaaggagt gttagcatga caccgccttt tttcagacgt cgccgtgaac
    10261 gggccagaac agccgccgct accgtaacct cctgaccagc cggtctaaaa gacagttcct
    10321 gcagctgcaa atagtggccg tgctttaatt gggagcgata aggttgtagg gggtgttgtg
    10381 ataaacttaa acctaaggtt tccgcctctg ccgttatcac ctcagcggca ctaaagtctg
    10441 gtaaagacgg cagcgtattc tcctctgtca gccaaggcca caagcttaat tgacccggtt
    10501 gacgatctgt ccacttcagc acatggtcca gggcagcgag tgcctgacga cgatttaacc
    10561 ctaaccccgc cagaccaccg gctttaacta aattagccag tgctttacgg cgtagagctg
    10621 aacgccccac ccgctgacag aaatcctgca gtgaagtata cggctcatgg gcccgggcgt
    10681 cgataatagt cgctgcaccc cgcgcaccta aatctcgaac taaagatagg gcggcgcgaa
    10741 tagcgctgcc ttccggttga aaagttatct cgctgtagtt aacatgaggt ggtaaaatct
    10801 caataccgcg gcgacgagct tcatgaatat agaccgctga accgtagtaa ccaaaaccgg
    10861 cagaaagtag agccgcataa tattctactg gataatgctg tttgagatat gctgtccagt
    10921 aggagaataa agcataggct gcagaatgtg ctttattaaa accataacct gcaaaacggc
    10981 acaaatgatc aaagatatct ccagcgtctt tggtgctgaa gcctttttgc accgccccag
    11041 taataaattt taggcggtgc acttcctgta atgaaggtgc ccgacgtccc atttcccgcc
    11101 gcaattgatc tgcttctccg gctgtatagc cgcctactgc acaggcaatc cgcattactt
    11161 gttcctgata aagtactaca ccatgtgttt cagccagtat ctcagcaagt gcagggtgta
    11221 aataagtggt ttcttcctga ccacgctgac gcgccacaaa ggtttccacc attccgcttt
    11281 cccaagggcc aggacggtag agtgacagta gatgaatcag atcctccaaa cgttctggca
    11341 ccagcaggcg taaaacacga cgcatgccgg cactttccaa ctgaaaacaa cccaggcttt
    11401 cacctcggcg cagcatggca aaggttgctt gatcatcagt gacgatcttt tccactgtgg
    11461 gcgcaccacc atgacgagca gccactgacg ctaaagtatc atgaataatc gttagattac
    11521 gtgagcccaa aacatcaagc ttgagaaaac ccatcgcctc cacatcatct ttatcccatt
    11581 gtgtcagtac ttcccctgct gggccgcggc aaaggggggc ggcttcagcc aaatctgcca
    11641 ggcccagaat aacgccactg gcatgagtgg aaagatgacg gcacaaacct tccagccgcc
    11701 gggcccccac taaaatagct tgcgccgtct catcgcgcaa gcgtgcctgc tgaaattccg
    11761 gaaactgtct taaggcggcc tcaataccgc cttgatcgga ataatggggc agcgcttcag
    11821 ccacagccga tactttgtca ccgctggcat ctaggatctt ccccacttcc cgcactgctg
    11881 cccgggcacc cagggtacta aacgtactca cctgtgccac cctaccctcg ccgtaacggc
    11941 tgcgcaaata gtctaaaagc tcaccgcgac gccgttgaca tacatcgata tcaatatcag
    12001 gaaattctac ccgatcttca ctgataaaac gttcaaaata gagtccatgt gctaaaggat
    12061 cgacttgagt tatgcccagc aaataggcta ctaaactacc accggctgaa ccgcgccccg
    12121 gtcctactgg aattcctgct ctccgggcat aattgactaa atcttgcaca attaaaaagt
    12181 aagcagccag acccttaggc aaaatcacgg ctaattcccg ttctaaacgc cgcagcgcca
    12241 cctctgctct cagtccggta cgctgcagtc cctcacggca taaacgtggc aaggcacttt
    12301 ccgccgcttc atcgtttggc aggtgaaagg ttggtaaatg tctgccttcc cgctgcaaaa
    12361 caaagctgca cttttgtgct aaaaagagag tagtatcaca ggcttgagga aagctggcaa
    12421 agcggcgcca catttctgct ggtgtcgcta aatgatagtt ggagccaggc aaaaaggcct
    12481 gcctagcacc cgccagcggt ttaccagtgc gaatacaatt taccatccaa aaaatacttt
    12541 cctcaccaga taaaacataa tgcacatttt gcgaggccag caaggaaaca ccttgggtct
    12601 gcgccagtgc tgctaactgc tggttaaaac gggatgttcc cggcaaacca gtatctgcaa
    12661 gggccaaata taattctgca gcaaaaatag gctgcaacat tgtcagtgct ttttctacag
    12721 tatttaagga aaaactttga taactgggat gtaaaacaag tgacagcccc tcgtaatgat
    12781 ctgccagttc ctgtggctcc agccacgctt tggctggttg ggataattga cagccgttga
    12841 tgagacgcag cagattgccc cagcctgttt tattacgcac cagcagcact acaggcaaac
    12901 ctgaactaag atttaactct acgcccaaaa ttggctgcag accatgttgt agtgctaact
    12961 gtgtaaaacg tactgcacca tgcacaacgc cataatcagt taacgccacg gccggcatgc
    13021 ccagctcctt tgcacgcagc accaattctt ccaaacggca ggcactgcgc agaaaactat
    13081 actcactatg cactcgcaaa tgcacaaagg acattttatc accccagtag tccaaacata
    13141 tgttcgtaat gctattataa ccggtggtga cgaattttac aactggggcc tgactaatgc
    13201 aatcagcgcc tcgccaaaca aacttctatt aaaaaagcag ccccaagtcc ttacccacag
    13261 ggactttaag ctgcttttgt ctaaataaca ctcaaggata agatttttac aatccggcaa
    13321 aaaatcaatg ttgattatac ggcctgccag agcattttac cgtccacaaa gctgtaagct
    13381 acttggttag tttgatgaag atgggaaaga caagcggtga tggtactaaa gttaaggaca
    13441 tactggatag catcaaatct tcgtttagta gttgtaatat agtaagctaa aatttcttcc
    13501 cgcgttaggg gctctttttg aataaaagta aggatttggg ccagtgtttt ctctatggcg
    13561 gcgatgtttt tagtaatgat ttctgaggtg tcgcttgtta cctcgccatg ggcaggtaca
    13621 taatactgaa agctgctgtg ctgtaatttt attagtgtct gtaaggccgc agaaacgtca
    13681 gcagtgtagg acatgttaaa cttgtccaag atcagctcgc caacataagc atcaccgcaa
    13741 aagcaaaccc catctgctgt caggacgcca atttgaccgg ggctatgtcc tgccagatca
    13801 aggatttcta gatcaatacc gagcatagta caaggccctg tttctagtac ctgctcgaca
    13861 ggagaggcgg gagccatcag aaaatgagat tttaattcat ttacaggtgc agcagaaaaa
    13921 aggtaaaagg gctccagctt tgtgttttca attaatgctt tttccagttg tgaagcacat
    13981 actatggcac cggcttcacg caacggggcg ttgccaccac aatgatcagc atgagaatgg
    14041 gtattgataa tagccttaac ccgtagtccg gcctgctgca gacagtgaag gatttgctgg
    14101 ccaacccctt tgtccaaacc agtatcaatc acgatacaat catttgcctt gattaacaca
    14161 ccaatattaa ccggactagg tatatagtag gtcttaccgc ttacttgttt taaggttaag
    14221 ctcatcgtct tccctcactt taagtcgcct gctctactat taaggctatt taggttacta
    14281 tgtacaacaa tttcttttag atcgagctgc ttaatactcc acgcccggtt gtgctttgat
    14341 acccgcttga taatgatgct tgatttcctt gacctcactg actagatcag ccgcttcaat
    14401 aatggactca caagcataac gcccagttag gacgacatta gcatgccggg gacgagcttt
    14461 aagcatggct aaaacatcat caagttcaag caaatcgtta tagagggcga aatttatttc
    14521 atcaaaaata accaggtcgt attcacccga cagtagtgct tccttacctt gagtaaagac
    14581 attggcggca tcagtataat cagcttgttc aaaatcacca tgtttacgaa aacggctgcg
    14641 ttctgactgc aaaactgtca tattgggtaa aaacttacga atcccttgta tttcaccgta
    14701 agtaggatca cttttacgaa actgcaccat aaatattttt tcaccatgac cgctagcgcg
    14761 taaagctaaa ccaagtgctg aggttgtttt ccctttccca ttgccagtat acaccatcac
    14821 aatgccttgt ctgtctttca tttgcttcac ctcattatta ataataagca ttaactttga
    14881 ttactggatc gtactatcca ctttagtgtg agctgaatcg ttttaattat taataggcta
    14941 atcctctccc taaaataaat tacacctatc ctacgttcag ataagtgtaa ataggggatc
    15001 tcaaatctag taccctggaa attttcccta tctgccgtaa ggaatgtgct ttgtaaaccc
    15061 caaagttgta tcctgactta agcatcacag cactatacag ccttcccagt ctcccagtgg
    15121 catcattgta tgtgctccgc tattacagtt gcgggacagt gtgggatttt cacccagctt
    15181 cctaacttac atattattgg tgtaacaagc tcattatatc tgatatattt agcgaataca
    15241 agcgatttca cgttgactgc tgtactgcaa tgtcgttcag catagaagca agtaagaggg
    15301 aatcaaaaaa ttgggccact tatatgctaa gtagcccaat ttatgtttta agtataagtt
    15361 attagctagc taattcttta actttaatag ctgcgccacc agcatcgcga gcataagcat
    15421 ctgcaccgat ttcagcagca aactcctgag ttatgggagc gccgcccacg attgttttga
    15481 agccggtaaa gccactttct ttaatagcag ctacggtgtc tctcatagag ggcatagtgg
    15541 tggaaaggag agcggataga gcaacaactt taacgtcagg attcgctttg attgtatcca
    15601 caaacgtttc tttagaaacg tcaacaccga ggtcaatcat ctcaaagcca gcgctttcaa
    15661 tcatcaggca aacaaggttt ttgccgatat catgtaaatc accttgtacg gtaccaataa
    15721 tacatttacc taaggagatc gagctgtccc ctgccagagc gggtttcaga atttcgacac
    15781 ctttttgcat tgctttacca gcgatcagca tttcaggtac gaatatttcg ccagctgaga
    15841 atttgtcgcc aacaacgccc atagcgtcaa ccatagcagc caaaatatct tttgctgcta
    15901 atccttcatc tagagcttcc tgtaccaaac cgggaactat ttttgtcttt ccggcttcaa
    15961 cttgagcctt aacttctgct agtttagaca tttatcttcc tcctcattaa taaatagttt
    16021 aagcaaaata acta
//
LOCUS       DASRMS010000113         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_700464, whole genome shotgun sequence.
ACCESSION   DASRMS010000113 DASRMS010000000
VERSION     DASRMS010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_700464"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(453..1355)
                     /locus_tag="VFC74_05365"
     CDS             complement(453..1355)
                     /locus_tag="VFC74_05365"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HZK24800.1"
                     /translation="MIGIDLNKPMEYRGSSLRFFKENEHHITRFCKEDVLLLVFAGCL
                     RFSENGIPYQINPGEYFIQRKNSMQRGDVPSQSPQYLYVHFLSQWSDSSAALPFRGTF
                     DYPAYKPMIAQLDRVGHSECSLTEKTMHFYQLLYALYTNQSKDTLADKIAHYIADQGQ
                     KDCSLEMLCKEFSFSKNYIISIFKEQYKMTPIEYRNHLKIEKAEYLLVVTSNSTEWIA
                     YQSGFNHYSHFYRLFFRKNGMSPKEWRSMKRVQPAAKGGNTGQPRGAPCDGQYFKEGT
                     NGFAKGQGNNGQIVTPKPQDRGAD"
     gene            1473..3284
                     /locus_tag="VFC74_05370"
     CDS             1473..3284
                     /locus_tag="VFC74_05370"
                     /inference="COORDINATES: protein motif:HMM:NF019556.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HZK24801.1"
                     /translation="MKKWSFYSTKEIAPAGWLKRQLELQAEGLSGNLDKIWPDIKDSG
                     WIGGAREGWERVPYWLDGFIPLAYLLENKDMMQRAEKYIRAIITHQQPDGWICPCSQE
                     ERKTYDTWAVLLMTKVLTVYYECTGDESVLTVVYATLKNFYQLLKDNAIQLIRWGKYR
                     WFEGFVAINLLHRKYSEPWIVDLAKILKKQGFDYHQAMPLWKTPLNHWKLETHVVNIA
                     MMLKSQALSNDILKEEDTQSAEQWYQALYQYNGTPVGLFTGDECLSGQSPIQGTELCA
                     VVEQMYSYEHLYAYTGQDKWMERLELAAFNALPATLSDDMWAHQYVQMTNQMACQEFP
                     GKAVFRTNGKDAHLFGLEPHYGCCTANFSQGWPKFALSTFMHRDDTIVNAIPVPSVLE
                     TEDIYISLETNYPFQTSFQYTIASKKDFHFEVRIPSFAQNLTVNGEKQAAKHQKFTIK
                     ANTKTQISINYTVTPALEKRPHDLNVVKYGSLIFAVPIAYEKQMHEYVKDGVTRKFPY
                     CDYQYIPKSHWSYAYCQGELAVENHPIGETPFSSQNPPITIAAKVKKIQWGLEDGYET
                     VCAKTPLSREPIGQMEEIQLYPYGCAKLRMTELPLLE"
BASE COUNT         1039 a          792 c          849 g          952 t
ORIGIN      
        1 tcaaaagacc acaccagaga atttagtctc caatgtggtc ttaatcttgc taacccttaa
       61 agctgttcac ttaagttttt atccaatccc tatgtgaaag cttctaaggt gtagccgtta
      121 tgaaaaagaa aaatgcaaag cttattaaaa atataacggc catactgatc gctgccggaa
      181 tatatgccct gattgcctat ggaatgagta ccaacgtcct tccccgtgcc acccaaagtc
      241 tgcttataaa ggcttgctac aatgttatat tggcactttc tttgaatttg gtggtgggct
      301 ttttagggga gctttctttg ggccacgcag ggttttatgc tttgggcgcc tatgcgggct
      361 gtattgtttc cattaatgtg aacctgccgg tggaactgcg gtttttactg ggcgtgttgg
      421 tgggtggggc ggttgctgct ctgggcggct ttttaatcag ctcctctatc ctgcggcttc
      481 ggggtgacta tctggccatt gttaccctgg cctttggcga aaccattcgt tccttcttta
      541 aaatattgcc catcacaggg ggcacctctg ggctgaccag tattccctcc cttggcagca
      601 ggctgcactc ttttcatact tctccactcc tttggcgaca taccgttttt gcgaaaaaac
      661 agcctgtaaa aatgagaata atggttaaac ccactttggt aggcaatcca ttctgttgag
      721 tttgaggtta ccaccagcaa gtactccgct ttttcaatct ttaaatgatt cctgtattca
      781 ataggagtca ttttatactg ctctttgaaa atgctgataa tatagttttt gctaaacgaa
      841 aactctttgc atagcatttc aagagagcaa tctttttggc cctgatcggc tatataatgg
      901 gcaattttgt cggccaaagt atccttggac tggttggtat atagtgcata taatagttgg
      961 taaaaatgca ttgttttctc ggtcagcgaa cactcactgt ggccaactct atcaagctgg
     1021 gcaatcatgg gcttgtatgc cggatagtca aatgttccgc gaaagggaag agccgcgcta
     1081 gagtctgacc actgggaaag gaaatgcaca tacaaatatt gaggggattg gctgggcaca
     1141 tcgcctcttt gcatggaatt tttcctttga ataaagtact caccggggtt aatttggtaa
     1201 ggaatgccgt tttccgaaaa ccgtaagcac cctgcaaaca ccagcagcaa aacatcctct
     1261 ttgcaaaagc gtgtgatgtg gtgctcgttt tctttgaaaa accttaggga agagcccctg
     1321 tattccattg gcttgttcag atcaattcca atcataaaat cacccttttc atcatagcgg
     1381 cattctgtga tattgtcaat gttttatgtg ctttacgcaa ttcctatagt tgcaagtcaa
     1441 tggtatactt tataaaaaag agaggtgtgc ctatgaaaaa gtggagcttt tatagtacca
     1501 aagaaattgc ccccgcaggc tggctgaaaa ggcaactaga gctgcaagca gagggcttaa
     1561 gcggaaacct agataaaatt tggcccgata tcaaagacag tgggtggata gggggagcgc
     1621 gcgaggggtg ggaaagagtg ccctactggc tggacggctt tattcctttg gcctacctgc
     1681 ttgaaaataa agatatgatg caaagggcag aaaagtatat acgggcaatc attacccacc
     1741 agcagcccga cggctggatt tgtccatgca gccaggagga gagaaaaaca tacgacacat
     1801 gggcggttct gctcatgacc aaggtgctta ccgtttatta tgaatgcacc ggggatgaaa
     1861 gtgttttaac agtggtgtat gccaccctaa aaaactttta tcagttgctc aaagacaatg
     1921 caatacagct tatacggtgg gggaaatacc gctggtttga agggtttgtt gcaatcaatc
     1981 ttttgcaccg gaaatacagt gagccatgga ttgttgacct ggcaaaaatc ctgaaaaagc
     2041 agggctttga ctatcatcag gcaatgcctc tatggaaaac acccttgaac cattggaagt
     2101 tggaaaccca tgttgtaaac attgccatga tgctaaaaag tcaggcacta tcaaatgata
     2161 ttctaaagga agaggatacc caaagcgcag agcaatggta ccaggcactg taccaatata
     2221 acggcacgcc tgtggggctg tttacggggg atgaatgcct ttctgggcaa agccccatac
     2281 agggaaccga gctttgtgcc gtggtggagc aaatgtattc ctacgagcat ctatatgcgt
     2341 ataccggcca agataaatgg atggaacggc tggaattggc cgcattcaat gccttgcccg
     2401 ccacactgag cgacgatatg tgggcgcacc agtatgttca aatgaccaat caaatggcct
     2461 gtcaggagtt tcccgggaag gccgttttta gaaccaacgg aaaagacgcg catttgtttg
     2521 ggctggagcc tcactatggt tgctgcactg caaacttcag ccaggggtgg cccaagtttg
     2581 ccctcagcac ctttatgcat agggacgaca ccatagtgaa tgctattccc gttcccagcg
     2641 tgctggaaac agaggatatt tacatttctt tggaaacaaa ctatcctttc caaaccagct
     2701 ttcaatacac cattgcatca aaaaaggact ttcattttga agtgcgcatt ccctcttttg
     2761 cccaaaacct tacggtcaat ggggaaaagc aagctgccaa gcatcagaaa tttaccatta
     2821 aagcaaatac caaaacgcaa atatccataa actatactgt aaccccggcc cttgaaaaaa
     2881 ggccccatga cttaaatgta gtgaaatacg gctcccttat ctttgctgtt cccatcgcct
     2941 atgaaaagca aatgcatgaa tatgtaaagg acggggtaac cagaaaattc ccctactgcg
     3001 attaccagta tattcccaaa tcccattgga gctacgccta ttgccagggg gagttggcag
     3061 tggaaaacca ccccatcggc gaaacgccct tttcctctca aaacccgccc atcacaatag
     3121 ctgcaaaagt aaagaaaatc caatgggggc tggaggatgg ctatgaaacc gtttgcgcca
     3181 aaacgccctt atccagagaa cccattggcc aaatggaaga gatacagctt tacccttacg
     3241 gatgtgcaaa gctgcgcatg accgagctgc cattgcttga atgaattagg gccaattttg
     3301 cctggtaaaa agaatagcgc ataggggagt ggggcggatt gcgtgaaaaa gcatcggctt
     3361 ttacagggtg tgctcttggg caaagtgcct ttagatttaa cttttccatg agaaaatcgt
     3421 aaaaattgct ttttaaataa ggaaccaacc gcaaaagaag aaaaggctgc atgcagcata
     3481 cacagcagtg cagtacgcac aagaagcgga acggctcaag ttacttttag ttgcaaaagg
     3541 tgctaaagat attgatgaat tcattaaata tctagaaaag ctgatgaagg aaaaaaagag
     3601 taaccggtca gacggaaccg attactttgg ga
//
LOCUS       DASRMS010000114        13355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_299074, whole genome shotgun sequence.
ACCESSION   DASRMS010000114 DASRMS010000000
VERSION     DASRMS010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 13355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13355
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299074"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VFC74_05375"
     CDS             complement(<1..401)
                     /locus_tag="VFC74_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HZK24802.1"
                     /translation="MYTEAELVQELKKGNEQAVAVLVERYSDKLLHLALGICGDLQRA
                     EEVVQDTMLQACRKIDSFAEKAALATWLYRITINLAKNSSRSFWLRRITTWEEQKMQM
                     LAAPVSSQPELMTIKKETNSQVIELLQQLPV"
     gene            588..1055
                     /locus_tag="VFC74_05380"
     CDS             588..1055
                     /locus_tag="VFC74_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24803.1"
                     /translation="MQQLERNHDFLYGLKAGVAGAVVKYGFNELMQLSGIAKYDNNAT
                     SLTVVFSAYQHSPFYWILGFLHAIMIGAFFGVCIAFVFDYILTPEDYLLKCLGIGVFI
                     YFFNFGVMSRAFHYPADITTLPGDVISMFLSLLIYAAVTGWTLKLQGFFQEIK"
     regulatory      1205..1368
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00380"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="The M-box (ykoK) riboswitch appears to function as
                     a genetic 'off' switch that is important for maintaining
                     magnesium homeostasis in bacteria; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="magnesium"
                     /db_xref="RFAM:RF00380"
     gene            1584..2924
                     /gene="mgtE"
                     /locus_tag="VFC74_05385"
     CDS             1584..2924
                     /gene="mgtE"
                     /locus_tag="VFC74_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HZK24804.1"
                     /translation="MEFAHVNELLAQKKFHQLKQYLANSQLADIAELIDQLELKTALL
                     VFRLLPKDEAAAVFAYISPLRQTELSQLVNEDELQQIINDLYFDDKLDFLEEMPATVV
                     KRIIANTSEKERTLINQFLHYPEDSAGSLMTIEYVDLKKEMSVQAALERIRQTAPDKE
                     TIYVCYITDASRHLEGTLSLRDLVVATPEQKISDIMHTEIIYAKTNDDKEYMADLVKK
                     YDLLAIPITDNEERLIGIVTVDDVLDVIEDENTEDFHRMAAMQPTNEEYLDSGVLKMA
                     RQRITWLLFLMISATFTGYIIRTYQATLETVVALTVFIPMLMDTGGNAGSQSSTLIIR
                     SLALNEVSIHDLVKILWKEFRVSIVVGFTLAFVNFFRILLIERYSVAVAATVSLTLVF
                     TVITAKIVGASLPIIAKQLKIDPAIMASPIITTVVDTISLIIYFGLATRIMMLG"
     gene            complement(3035..4861)
                     /locus_tag="VFC74_05390"
     CDS             complement(3035..4861)
                     /locus_tag="VFC74_05390"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZK24805.1"
                     /translation="MNILLIGNPNVGKSAIFSHLTGVSVTTSNYPGTTVQFTKGSLTY
                     QGQEYEVIDIPGTYQLTPEAEAEKVAASMISEGDILVNVVDATNLERNLNLTLQLMEF
                     GKPMVLVLNMWDDAQHKGIEIDQELLAALLEIPVLTTNGLTGEGLNQLPSVCLSAQPV
                     SVPKLLPQQRWSKIGSIISSVQNLSHRQHTFAERLQDFSIHPWFGPPLALFILYWAFK
                     IVISSGEMLTNFTTNLFDRVYAPFILALSKILGGEGLLHALLLGDINGTSIDYEGAMG
                     VLTTGVFVTLGIVLPYIVLFYLVFGFLEDLGYLPRVAIILDRLLHKVGLHGYSVIPMF
                     LACGCNVPGVLAIRNLESRRERFITAVMTCTTIPCMAQTSLIVKAVGAQGGIFVLLLF
                     VTLFTVWISMGMLLKQLVEGTTPTLLMEVPPYRMPNLTIQIKKLWLRVKCFLKEAIPY
                     VLGGIALINLLHVSGVISWLGHLFSPLVKGVFGLPEQTVAALIIGIVRKDAAVALLEP
                     LGLAGVQMVTAVVVLTLYFPCIATFTVLLKELGLKDTAKAVAIMFAVTLTAGGGLNLL
                     SKLYNPWVIIAVEFILVIILALCIQLFFSARQADHIDRSFTQ"
     gene            complement(4858..5103)
                     /locus_tag="VFC74_05395"
     CDS             complement(4858..5103)
                     /locus_tag="VFC74_05395"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZK24806.1"
                     /translation="MQKSKLPLSALKKEETGVVVFVQGGESMRRRLEALGIRLGAKIK
                     KKSKLFGLGPVIVSVGNAEIAVGYGMVTRIFVEVDRK"
     gene            complement(5174..5605)
                     /locus_tag="VFC74_05400"
     CDS             complement(5174..5605)
                     /locus_tag="VFC74_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HZK24807.1"
                     /translation="MLQQLYLYLNEHNLRVTQQRRAILQVLYENRDRHLETENIYELL
                     AQKGVTARKIGLSTVYRTLELFARIGLASKLSFGKAPARYELIMQNKNGHHHLICLRC
                     GAVQEINDHKIHDFKCQILRETGFEVAEKPMKIYGYCQQCR"
     gene            5868..6209
                     /locus_tag="VFC74_05405"
     CDS             5868..6209
                     /locus_tag="VFC74_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002599588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmaF family protein"
                     /protein_id="HZK24808.1"
                     /translation="MSDLEIQHKNRQRHVHEMIGSVELAEYEEDPHGHRFATVTDEAI
                     LTKPGDHIHKVKFRTDFYEDHYHEFHGKTSGAIAVGDRHIHYLESVTTVDDGHKHHFR
                     VAALIENPTGE"
     gene            complement(6270..6357)
                     /locus_tag="VFC74_05410"
     tRNA            complement(6270..6357)
                     /locus_tag="VFC74_05410"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6321..6323),aa:Leu,seq:taa)
     gene            complement(6370..6443)
                     /locus_tag="VFC74_05415"
     tRNA            complement(6370..6443)
                     /locus_tag="VFC74_05415"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6409..6411),aa:Cys,seq:gca)
     gene            complement(6569..6643)
                     /locus_tag="VFC74_05420"
     tRNA            complement(6569..6643)
                     /locus_tag="VFC74_05420"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6609..6611),aa:Gly,seq:gcc)
     gene            complement(6648..6723)
                     /locus_tag="VFC74_05425"
     tRNA            complement(6648..6723)
                     /locus_tag="VFC74_05425"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6688..6690),aa:Phe,seq:gaa)
     gene            complement(6730..6806)
                     /locus_tag="VFC74_05430"
     tRNA            complement(6730..6806)
                     /locus_tag="VFC74_05430"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6770..6772),aa:Asp,seq:gtc)
     gene            complement(6874..7521)
                     /locus_tag="VFC74_05435"
     CDS             complement(6874..7521)
                     /locus_tag="VFC74_05435"
                     /inference="COORDINATES: protein motif:HMM:NF003989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HZK24809.1"
                     /translation="MPRRDAPKSVVKRLPVYLRILDNLIRRDVEVVSSRILSDETGFT
                     AEQIRKDLAHFGAFGTRGTGYNTNYLRDKLLRIIGLHRTTKIIVVGYGHLGKALTRYN
                     AGKNPYVEIVAAFDIDPKEQKTELEGLRVLGMDMLPEVIRAEDVKVTILTVPAEVAQE
                     VVDLLVENGITAVLNFAPTNINAPENVYVHNTDLTIELQTLIYYSLEDEMHQRGE"
     gene            complement(7737..8294)
                     /locus_tag="VFC74_05440"
     CDS             complement(7737..8294)
                     /locus_tag="VFC74_05440"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK24810.1"
                     /translation="MKKPLKYAQELVQTVVSAGDVVVDATCGNGHDTVFLAALVGTAG
                     QVYAFDIQPQALAATKKKLLDSQLQAQVKLWEADHADIAFWPQAPLAAVMFNLGYLPG
                     GDHALVTRAETTVRALQIAVARLKEDGVITLMVYRGHPGGEQEYQALLNYCQFLPQQN
                     YAVLEYQFINQQHQPPLLLAISRLP"
     gene            complement(8335..9036)
                     /locus_tag="VFC74_05445"
     CDS             complement(8335..9036)
                     /locus_tag="VFC74_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HZK24811.1"
                     /translation="MHWPLLCRTVFLYLAVLVAIRIMGKREVGELSAFDLVVAIMIAE
                     LAAMSMERIDMPLHEGLVPIFTLVVLEVFLSYLSLKSHAIRGLIDGAPSIVIANGKII
                     EKELRKLRYNVSDLIGQLREKDVPNIADVQYAVLETSGELSVVLKGNKRPVTPEDLGL
                     PAKYEGIPTPLIFDGHIHYNNLRNLHLDESWLLAEVRKYGLERIEDVLYGSLDTDGNL
                     YVSEKERATKKKPAG"
     gene            complement(9095..9646)
                     /gene="pyrR"
                     /locus_tag="VFC74_05450"
     CDS             complement(9095..9646)
                     /gene="pyrR"
                     /locus_tag="VFC74_05450"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HZK24812.1"
                     /translation="MQIKKQIMDAEAMRRALMRISHEIVEHNKGMAGLVLVGIRTRGV
                     PLAERIAHNIGIIEGVEPQVGLMDITQYRDDLSRLPAQQQSRETIVNVPVTDSKIVLV
                     DDVLFTGRTIRAAMDALMDLGRPQSIQLAVLVDRGHRELPIRADYVGKNVPTSHLENI
                     AVVLSEVDADSDKVLIIESQHVT"
     gene            complement(9661..10584)
                     /locus_tag="VFC74_05455"
     CDS             complement(9661..10584)
                     /locus_tag="VFC74_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010292314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HZK24813.1"
                     /translation="MKTFTYQVAGELVGERLDVAVTKLIPELTRARVQKLITEKLVTV
                     NDTARKANHRLREEEVITVTIPDVKPSDLQAEEIPLKILYEDADILVLNKPKGIVVHP
                     AAGHATGTLVNALLYHCQDLSGIGGEARPGIVHRLDKDTSGVLVIAKNDQAHLKLSRQ
                     FKAHTVTREYVAIVHGQLTAAHGTIDAAIARHPQERKKMAVTKRDKGRRAVTHFEVLE
                     SFKDYTYVALRLETGRTHQIRVHLASLGHPVVGDPVYGYKKQRFKLHGQALHARLLGF
                     KHPSSGSYLEFSSEPPQEFKEVLASIQELER"
     gene            complement(10581..11327)
                     /gene="lgt"
                     /locus_tag="VFC74_05460"
     CDS             complement(10581..11327)
                     /gene="lgt"
                     /locus_tag="VFC74_05460"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HZK24814.1"
                     /translation="MFPVLLRIGSFEVYAYGVMLALAFGVGTIGLTRAGRQVGLPEEK
                     LLDLTIRIMVAAIIGSRLLYVLLELPVYLANPWGIFKLRSGGLSFHGGLAAGVAVGLW
                     YTHRHGLPQGQVADLAAPYLALGYGIVRIGCLLNGCCYGRPTDLPWALPAAYHDGVLR
                     HPTQLYAAAAACLIFFILRWRKKHTRFVGQLFLEFVLLYSIYRFIVEFFREVSSFVGF
                     LTLGQAVSIVGAVAAFVAIRIWPLGRGERR"
     gene            complement(11327..11773)
                     /gene="lspA"
                     /locus_tag="VFC74_05465"
     CDS             complement(11327..11773)
                     /gene="lspA"
                     /locus_tag="VFC74_05465"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZK24815.1"
                     /translation="MPTLLLMAVLLLLDQGTKWYVTQKLSVRQSIPVIKNVFHITYIR
                     NPGAAFSILSHRPALLIVIATVVTIGLVVFFYSVPVEQKLLRLALTFAIAGALGNLID
                     RLRLGYVVDFLDFRVWPIFNVADIAIFMAIGLLVLDLARTPGGKGI"
     gene            complement(11930..12541)
                     /locus_tag="VFC74_05470"
     CDS             complement(11930..12541)
                     /locus_tag="VFC74_05470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HZK24816.1"
                     /translation="MDSESFRHRQQLEQLKNDLLEQTEFTEGLQEHEGLRDSTQELSV
                     VDNHPADVASENYERAKDISLHEKNQLVLKLVEEALEKLAVGTYGVCENCGQLIAAER
                     LDAMPYAEFCLDCKKRAEGPLHDFPRPIEELNLMSPFARTYMDEKDYTGYDGEDAWQD
                     VDQYNKLPHVFYRETVDESDDTIGAVEDTDRISNEEYRKQLPD"
     gene            complement(12551..12853)
                     /locus_tag="VFC74_05475"
     CDS             complement(12551..12853)
                     /locus_tag="VFC74_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5665 domain-containing protein"
                     /protein_id="HZK24817.1"
                     /translation="MEGPSKPDEFLLKRLNHRVARLLQEIDKFNIAEYLNLLNNPRRF
                     FWVNFLGGVGRGVGAAIGATVVAAVVISLLRRLVVLNLPIIGDYIVELVQIVQNHL"
     gene            complement(12996..>13355)
                     /locus_tag="VFC74_05480"
     CDS             complement(12996..>13355)
                     /locus_tag="VFC74_05480"
                     /inference="COORDINATES: protein motif:HMM:NF018531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HZK24818.1"
                     /translation="ALLTYRDVVTRRLEEARQEKRLGSSLTAALELYPDQTAYDALLP
                     FKERLAEIFIVSACTLHEPDAGAAETVVEQGLGVAVKTAAGEKCERCWMIHPQLGIDA
                     THPTLCPRCTESVIAQS"
BASE COUNT         3837 a         3258 c         2599 g         3661 t
ORIGIN      
        1 ttaacaggca gttgctgcag cagctctatc acttgactat ttgtttcctt cttgatcgtc
       61 atcaactctg gttgtgaaga caccggtgcc gctagcattt gcattttctg ctcttcccag
      121 gttgtgatgc gacgcagcca aaaactgcgc gaactatttt ttgctaaatt aatagtgatg
      181 cgatagagcc aagtagccag tgccgctttc tccgcaaaac tatcaatctt ccgacaggcc
      241 tgcagcatag tgtcctgtac cacttcttcc gctctttgca gatcaccgca gatacctaaa
      301 gcgagatgca acagtttgtc actatagcgc tctactaata cggccactgc ctgttcattg
      361 cccttcttca attcctgtac aagttcagct tctgtataca tctaatcctc caggcctgat
      421 tctacttatt agacacaccg tcgtttaatt tggttgcact taactaactt cccactgtaa
      481 aattaatttc cttcgtcctt ctataattgc taatactgtg gggatactaa aaaagcatgc
      541 tcatgcaaaa ataaaaatgt aaattgttgt cactcaggag gctgaatatg cagcaactgg
      601 aacgaaatca tgactttctg tatggactaa aagccggtgt agctggagcg gttgtcaaat
      661 atggcttcaa cgaattaatg cagttaagcg gcattgctaa atacgacaac aacgctacat
      721 ctcttacagt agtcttttct gcctatcaac actcaccctt ttattggatc ttaggttttc
      781 tacatgctat catgattggt gctttctttg gcgtctgtat tgcttttgtt tttgactata
      841 ttctcacccc agaagattat cttctaaaat gtttaggtat tggggtattt atctacttct
      901 ttaattttgg tgtaatgtcc agagcctttc attatcctgc cgatatcaca acattaccgg
      961 gtgatgttat ctccatgttt ttaagcttat taatttatgc agctgtaact ggctggacat
     1021 taaagctgca aggcttcttc caagaaatca agtaactcac cactatgcag agacttgctc
     1081 acaatctgct cttttacaga tgatgtgcta actaattaca gctagtggcc gccatgacta
     1141 aaaaaaattt tctcatcatc tgttttaggc ttggctttaa agcccacgaa gcatataata
     1201 gtatcgtaca ctctgttagg tgaggttact atacaaacat atgctgctgc cctgaaacat
     1261 cgagagatgc caaagggtga gcaggtaata tcggtttaag gtattactta acgcagctgg
     1321 gttgccccta cgttgtatag tgctaaagct ttgacaaggg agtttataac ttcgctttgt
     1381 taacagcctc aactaattag agtggggctg tttatttttg cgcaaagtta attatttgga
     1441 ggtgatttgt atggacgctg accctgggag tcctgctcga ttatataggc aacatcaggc
     1501 tttcctgcaa gtcatcctta atattaatta ttgatcttgc tagcagggga gcctcaaatt
     1561 taattaagga ggcttaactc actatggagt ttgcacatgt caatgaactg ctggcccaaa
     1621 aaaagtttca tcaattaaaa cagtatctgg ctaacagtca attagcggat attgcagaat
     1681 taattgacca actagaatta aaaactgcgc tgctagtttt tcgtctcctg cctaaagatg
     1741 aagcggcagc tgtgtttgct tacatctctc ctctccgcca gactgagtta tcacaactag
     1801 taaatgaaga cgaactacaa cagatcatta atgatttata ctttgacgac aaattagatt
     1861 ttttagaaga aatgcccgcc accgtagtca aaagaattat tgccaatacc agtgagaaag
     1921 aaagaacact aattaatcag tttttgcatt acccggaaga ctccgccggc agcttgatga
     1981 cgattgaata tgtggatctc aaaaaggaaa tgtcagtgca agccgcactg gagagaattc
     2041 gtcagaccgc acccgacaag gagactatct acgtttgcta catcaccgat gctagccgtc
     2101 acctggaagg gaccctttcc ctgcgtgatt tagtggttgc tacgccagag caaaaaatta
     2161 gtgatattat gcatactgaa atcatttatg ctaagaccaa tgatgataaa gagtatatgg
     2221 ccgatttagt aaaaaaatat gatttattag ccataccaat cacagataac gaagaacgcc
     2281 tcatcggcat tgttacagta gacgatgttc tagatgtaat cgaagatgaa aatacggaag
     2341 actttcatag aatggcggca atgcaaccaa ccaatgaaga atatttagat agcggtgtgc
     2401 tcaaaatggc acgccaacgt atcacctggc tgctttttct catgatttct gctaccttta
     2461 ccggctatat tattcgcact tatcaggcga cattagagac agtggtcgcc ttaactgtct
     2521 ttatccccat gctcatggac acaggcggca atgcagggtc acaatcctcc acgctgatta
     2581 ttcgcagttt agcgttaaac gaagttagta ttcacgatct ggtcaagatt ctctggaaag
     2641 aattccgagt tagtattgta gttggcttca ctttggcttt tgtcaacttc tttaggattc
     2701 tactaatcga gcgctactca gtagccgtgg ccgctaccgt ctccttaact ttagttttta
     2761 ccgtgatcac ggccaaaatc gtgggcgcaa gtctccctat tatagctaaa cagctcaaga
     2821 tagatccggc aattatggct agtcccatca ttaccaccgt tgtcgacaca atctcgctga
     2881 taatttattt tgggctggct accagaatta tgatgttagg ataatccaaa ataagaacta
     2941 aaaataaggc cattgattag ttttcataat caacggcctt tccttttctc gcctactctg
     3001 ctgcaaaaca tgctaggata ttgcagagtt taagttactg ggtgaaagag cgatctatat
     3061 ggtctgcttg tcgagcggaa aagaacagct ggatacataa cgctaagatt attaccagta
     3121 taaattcaac ggcgataatc acccaggggt tataaagctt acttaaaaga ttcaatcccc
     3181 cgccggcagt caatgtcact gcaaacatta tagcaactgc tttagccgta tccttgaggc
     3241 caagttcttt taataagaca gtaaaagtgg cgatacaggg aaaatagagt gttagtacaa
     3301 ccacagctgt caccatttgt acacctgcta ggccaagggg ttccagcaaa gcgactgccg
     3361 catcctttct aacaatacca ataatgaggg cagctacagt ttgttccggc aaaccaaaaa
     3421 cacctttaac cagcggagaa aacaggtgcc ccaaccaact gattacacct gagacatgta
     3481 aaagattgat cagcgcaatg cctcctaaaa cataaggaat ggcttccttt aaaaaacatt
     3541 taacccgcag ccaaagtttc tttatttgaa tggttaagtt tggcatcctg tatggtggta
     3601 cttccatcaa gagagtcggc gttgttcctt ccaccagttg tttaagcaac atccccatac
     3661 taatccagac agtaaataag gtcacaaaaa gcagcagcac aaatattcct ccctgcgccc
     3721 ctactgcctt aacaattaac gaagtctgcg ccatacaagg aatagtggta caggtcatga
     3781 ccgccgtaat aaaacgttcc cttcttgatt ctagatttct aatagccaag acccccggta
     3841 cgttacaacc acaggctaaa aacatgggga tcaccgaata gccgtgcaaa cccactttat
     3901 gcagtaatcg atccaaaata atagctacac ggggtaaata cccaaggtct tccaaaaagc
     3961 caaagactaa atagaaaaga acaatataag gcaaaacaat ccccaaagta acaaacacgc
     4021 ctgttgtcaa tacacccatg gctccttcat aatcaattga agtaccattt atatccccta
     4081 gcaagagagc atgaagcaac ccctctccgc ctaatatttt acttaatgcc agtataaaag
     4141 gagcatacac tcggtcaaat agatttgtcg taaagttggt taacatctca ccgctactta
     4201 tcactatctt aaaggcccaa tagagaataa atagagcaag ggggggacca aaccaaggat
     4261 ggattgaaaa atcctgaagc ctctcggcaa aagtatgctg acgatgagaa agattttgca
     4321 cagaactgat aatactgcca attttactcc atctttgttg cggcaacagc ttgggtacag
     4381 acacaggttg ggcacttaaa catacagacg gcagttggtt taaaccttca cctgtcaacc
     4441 cgtttgttgt caggacaggt atctcgagta acgcggcaag caactcttgg tctatctcaa
     4501 tacccttatg ttgagcatca tcccacatat ttaggactag caccattggc ttaccaaact
     4561 ccatcagctg caatgtcagg ttaaggtttc tttccaaatt tgtggcatca acaacattaa
     4621 caagaatatc accctcgctg atcatcgacg ccgcaacctt ttctgcctca gcctcggggg
     4681 tcagctggta tgtaccagga atatcaatta cttcatattc ctggccctga taagtcaagc
     4741 tgccttttgt aaattgtaca gtagttcctg gataattgga cgtagtaaca ctcacacccg
     4801 ttaagtggct aaagatcgcg ctcttaccaa cgttgggatt accaatcagt aaaatattca
     4861 tttcctgtcg acctcaacaa aaattcttgt taccattcca taacctaccg caatttccgc
     4921 gttaccaacc gacacaatga ccggtcctaa accaaaaagt ttacttttct tttttatttt
     4981 agctcctaac cggatcccta aagcctccag ccggcgtcgc atactttcgc ccccctggac
     5041 aaacaccacc acaccagttt cttctttttt caatgcggag agtggtaatt tacttttttg
     5101 catacctcta ctacacccct ttgctaatga taatcatttt caattaacag agaaaaaaaa
     5161 tataccctga agttcacctg cattgctggc agtaaccata aatcttcatc ggcttctctg
     5221 caacttcgaa ccctgtttcc cgcagtattt ggcacttaaa atcatgtatc ttgtgatcat
     5281 taatctcctg cactgctcca catcgtaaac agattaaatg atgatgccca ttcttattct
     5341 gcataatcaa ctcataccgt gccggcgctt taccaaaaga taatttagaa gccagaccta
     5401 ttctcgcaaa taattccagt gtcctatata ccgtagataa gccaattttc ctcgctgtta
     5461 ctcctttttg tgccaatagc tcataaatat tctccgtctc tagatgacgg tcacggtttt
     5521 cgtataaaac ttgtaaaata gctctccgtt gctgcgtgac ccgcaaatta tgttcattta
     5581 aataaaggta cagttgttgt aacaattgcc tcccccttgc tagccagagg aatctggttt
     5641 atgagctaat gctcataacc atcttaccat gaatatttgc aataatcaag caataattag
     5701 tctaaaatta ttcctgcgct actagcaaac gatagataaa aaaagtcaac ccggtcacat
     5761 tcttacctat catacatact gtaacaaggg gaagactttg ttgcattctt ttatctgcac
     5821 attgagtatt ccttaatatt tctagatcgt agaaaggagc agtttggatg tcggatttag
     5881 aaattcaaca caagaacagg caaagacatg tgcatgaaat gatcggtagt gttgaacttg
     5941 cagaatatga agaagatccg cacgggcacc gctttgccac agtcactgat gaagctattc
     6001 tcaccaagcc gggtgatcat attcacaaag tcaagtttag aacagatttc tatgaagatc
     6061 attatcatga gtttcacggt aaaacttctg gtgcaattgc agttggtgac aggcatattc
     6121 attatctgga atctgtaaca acagtagatg atggacacaa acaccacttc cgtgttgctg
     6181 cactgataga aaatcccact ggcgaataaa tacgaaaaga ccacctgcat aggtggtctt
     6241 taaaaatggc atacagcctg taattcaagt ggtgccggag accggaatcg aaccggtacg
     6301 gtcttttgga ccgagggatt ttaagtccct tgcgtctgcc tgttccgcca ctccggcagg
     6361 attaataaat ggaggcgaca cccggatttg aaccgggggt aaaggatttg cagtcctctg
     6421 ccttaccact tggctatgtc gccccattag cctgcttaag cattcatatt aagcattcat
     6481 aataatagca tataggtgac cgggcgtcaa gaccggtcgt tattggatta aggaaggcgc
     6541 acctagtgcg ccgtcgtgtt tttttgaatg gagcggaaga cgggattcga acccgcgacc
     6601 ctcgccttgg caaggcgatg ctctaccact gagctacttc cgcatcatgg tgccgagacc
     6661 cagaattgaa ctggggacac gcggattttc agtccgctgc tctacctact gagctatctc
     6721 ggcataaaat ggcggagctg acgggatttg aacccgcggt ctcctgcgtg acaggcaggc
     6781 atgttaggcc tctacaccac agctccgcat atgcgatact tttctagtat acacaagggt
     6841 aaactaaaag tcaataacaa gtcatgagga aaattattcc ccccgctgat gcatctcatc
     6901 ctctaatgag tagtagatta aagtctgtaa ttcgatcgtt aaatccgtat tatgaacata
     6961 aacattttcg ggggcattga tatttgtggg tgcaaaattt aggacagctg tgatgccatt
     7021 ttccaccagc aagtccacca cttcctgcgc tacctcagct ggcaccgtca agatagtcac
     7081 ttttacgtct tcagctcgga taacctcggg cagcatatcc atgcccagca cacgcaaacc
     7141 ctcaagttct gttttttgct ccttaggatc gatgtcaaag gcagctacta tctctacata
     7201 gggatttttc ccggcattat aacgagttag tgctttacct agatgaccat atcccacgac
     7261 aatgattttt gttgtccgat gtaagccaat aatgcgtagt aatttatccc tcaggtaatt
     7321 agtattatag cccgtaccac gcgtaccaaa agcaccgaaa tgggcaaggt ctttcctgat
     7381 ctgctccgcg gtaaaacctg tttcatcact aaggatacgt gaggaaacca cctcgacatc
     7441 acgacgaatg aggttatcca agatccttaa gtaaacaggt agacgcttca caactgattt
     7501 aggcgcatct cgtctaggca tgttatcttt cccttcttat ccatctaatt ttgctggcct
     7561 gtaaagtttt acatgtttag taattgtatc ataatttata aaagaaaaaa ggtcgcaagc
     7621 attttctttt tttaaaacag atgtactact taaaacagat atgctagtta atattcacgc
     7681 tgcagctgga attccctgcc tgacgttggt aaccttttca cattctttga taatttctat
     7741 ggcaagcggc tgatagccaa gagtaaagga ggttgatgct gttgattaat aaactgatat
     7801 tctaatactg catagttttg ctgaggtaag aattggcaat aattcagtaa cgcctgatat
     7861 tcttgctccc ctccgggatg tcccctataa accatcaggg tgatgacgcc atcttctttt
     7921 agacgtgcaa ctgcaatttg cagtgctcta acagtagtct ccgctcgtgt caccagtgca
     7981 tgatcaccgc ctggcaaata tcctaaatta aacatcactg ctgccagcgg agcttgaggc
     8041 caaaaagcaa tatcagcatg atctgcttcc catagtttca cttgagcctg cagctgactg
     8101 tccagcaact ttttttttgt agcagcaagc gcctgtggct gaatatcaaa agcataaact
     8161 tgacctgccg tgccgactaa agcagccaaa aagactgtgt catggccatt gccacaggta
     8221 gcatcgacaa caacatcacc tgcgcttacc accgtttgta ctaattcctg tgcatatttt
     8281 agtggttttt tcatgctatc cctccaccag aaagtatggt gaaagcctgc ccagttatcc
     8341 ggcaggcttt ttcttcgtcg ctcgttcctt ttcactgacg tacagattac catcagtgtc
     8401 caaacttcca tacaagacat cttcaatgcg ctcaagaccg tacttgcgca cttctgccaa
     8461 taaccaactc tcatccaaat gtaaattacg taaattatta taatgaatat gcccatcaaa
     8521 aatcaagggc gtcggtatgc cttcatattt tgccggcaag cctaaatctt ccggtgtcac
     8581 cggtctctta ttgcctttaa gcaccacact taactcacca gatgtttcca agacggcgta
     8641 ctggacatca gcaatattag gaacatcttt ttcccgcagc tggccaatca ggtcagaaac
     8701 attatagcgc agcttacgca gttctttctc tataatttta ccattagcaa tcacaatact
     8761 cggagcacca tcaattagac cgcggatagc atgacttttt aagctcaagt atgataagaa
     8821 cacttctaaa accactaaag taaaaatagg tactaatcct tcatgaagcg gcatatcaat
     8881 ccgctccatc gacatcgctg ctaattcagc aatcataatt gctactacca agtcaaaagc
     8941 tgataattct cctacttcac gctttcccat aatgcggatc gccactaaca cagctaggta
     9001 caaaaagaca gtccgacaaa gtaaaggcca atgcaagctg cagcactctc cagtatcttt
     9061 attctatcta ctagctttac ccagctggca agcattatgt aacatgctgt gattctataa
     9121 tcagaacttt atcgctatct gcatctactt cgcttagcac cacagcgata ttctccaagt
     9181 gacttgtggg cacattctta ccaacataat cagcccgaat cggtaattcg cggtgaccgc
     9241 gatcaactaa tactgccagt tgaatcgact ggggccggcc taagtccatc aaggcgtcca
     9301 tcgccgcacg aatagtacga ccagtgaaca gcacatcatc gactaacacg attttgctgt
     9361 ccgtaaccgg cacattaaca attgtttccc tgctttgctg ttgtgccggt aagcgggata
     9421 gatcatccct atattgtgtg atatccatca agcctacttg aggttctacc ccctcaatga
     9481 tgccaatatt atgcgcaatg cgctcggcta agggcacacc ccgtgtgcga ataccgacca
     9541 agactagccc ggccatgcct ttattgtgtt ccacaatttc atgggaaatg cgcatgaggg
     9601 cacgacgcat ggcctcggca tccataattt gtttctttat ttgcatttac atacccccct
     9661 ctacctttca agctcttgga tactagccaa tacttcttta aactcctggg gcggttcact
     9721 gctaaactcc agatagcttc cgctgctagg atgtttaaaa cctaaaaggc gagcgtgcaa
     9781 agcctgacca tgtaatttaa agcgttgttt tttatagccg taaaccggat caccaaccac
     9841 agggtgtccc agggaagcta aatgcactcg aatttggtgt gtacgacccg tttccaaacg
     9901 caaagctaca tatgtgtagt ccttaaagct ctccagcact tcaaagtgtg tcaccgcacg
     9961 gcgaccttta tcccgttttg tcaccgccat tttctttcgc tcttgtggat gacgggcaat
    10021 ggcggcatcg atggtcccgt gtgccgccgt cagctggcca tggacaatag ccacatattc
    10081 cctagttaca gtatgagctt taaattggcg tgataactta agatgcgcct gatcattctt
    10141 ggcaatcacc agcactcccg aagtatcttt atctaaacga tgtacgatac ctggtctggc
    10201 ttccccgccg atgcctgaaa gatcctgaca atgatagaga agtgcattaa ccaacgtacc
    10261 ggtggcatgc cctgccgccg gatgtaccac tatgcccttc ggcttattta atactaaaat
    10321 atccgcatcc tcatagagaa tctttaaggg aatctcttcg gcctgcagat cagaaggttt
    10381 gacatcagga atagtaactg taatcacttc ttcttcgcgc agccgatgat tcgctttacg
    10441 cgcagtatca ttaactgtca ctaatttttc ggtaattaat ttttgcacac gagcccttgt
    10501 tagttcaggt attagtttcg tgacagccac atctaaacgc tctcccacta actcgcctgc
    10561 cacttgatag gtaaaagttt tcatctacgc tcaccccgtc ctagcggcca gatgcgaata
    10621 gccacaaaag cagcaaccgc tcccacgata ctaacagctt gtcccaaagt gagaaaaccc
    10681 acaaaagaac tcacttcacg aaagaattcc acaataaaac gataaatact ataaagaagt
    10741 acaaactcca aaaacaactg tccaacaaag cgggtatgct ttttgcgcca gcgtaaaata
    10801 aaaaagatta ggcaagcggc agcggcagca tagagctgcg tagggtgtct taacacacca
    10861 tcgtggtaag cagcaggcaa agcccagggt aaatccgtcg gccgcccata gcagcagcca
    10921 ttcagtaggc agccaatccg tactattcca taccctaaag ccagatacgg tgcagctaaa
    10981 tctgctactt gaccctgcgg taagccgtgg cgatgcgtat accataatcc cactgccacc
    11041 ccggccgcta aacctccgtg aaaagagagc cccccactac gcagcttaaa gataccccag
    11101 ggattagcaa ggtagacagg tagttccagt aaaacataaa gtaggcggga accaataatg
    11161 gccgccacca taatcctaat ggtcagatcc aatagttttt cttctggcag gccaacttga
    11221 cgtcctgccc gggttaaacc aatggtaccg acaccaaaag ctaaagccag catcacacca
    11281 taagcataaa cctcaaagct gccaattcgc aataatacgg gaaacattaa attcctttcc
    11341 ctcccggtgt acgcgctaaa tcaagcacca gcaaaccaat cgccataaaa atagcgatat
    11401 ccgccacatt aaagattggc cagacccgaa aatctaaaaa gtccacaaca tatcccagcc
    11461 gcagtcggtc aattaaatta cccaatgctc cagcaatagc aaacgtcaag gcgaggcgca
    11521 gcagtttttg ctccacaggt acactgtaga aaaaaacgac cagaccaata gtcaccactg
    11581 tggcaatgac aatcagtaaa gcgggacggt gagacaaaat tgaaaaagcg gccccggggt
    11641 tacggatata ggtaatgtga aagacatttt taatcacagg aatactttgt cgtacgctta
    11701 gtttctgtgt cacataccac ttggtaccct ggtccaaaag cagcaagaca gccatcagta
    11761 gtaaggttgg caatgcataa caccccagca ttcatttcct gatttccttt attctaccat
    11821 aagtgctgct ctgccgacaa ctgataagta ggctgcactg cttaatctag cgaagcctta
    11881 acctgaccct actgtttatg cagcaaacag ctttaacttt tttcactgct taatccggga
    11941 gttgcttacg gtactcctca ttagagattc tatctgtatc ctctacagca ccgatagtat
    12001 cgtcactttc atctacagtt tcccggtaaa aaacgtgagg aagtttatta tattgatcga
    12061 catcctgcca ggcatcttca ccatcatagc cggtataatc cttttcatcc atataagtac
    12121 gggcaaaggg tgacatgagg tttaactcct cgataggacg ggggaaatca tggagcggcc
    12181 cttccgcacg ctttttacag tccagacaga attcagcata gggcatagca tccagacgct
    12241 ctgcggcaat taactgtcca caattttcac atacgccata tgtgcctaca gccagttttt
    12301 ctagcgcttc ttccaccagc tttaacacca gctgattttt ttcgtgcagc gagatatctt
    12361 tggcccgctc ataattttca ctggctacat cagcaggatg gttatcaacg acagataatt
    12421 cttgcgtaga atcacgtaat ccttcgtgtt cttgcaaccc ttccgtaaat tccgtttgct
    12481 ctaacaaatc atttttcagc tgctccagct gttgtcgatg gcgaaagctt tctgaatcca
    12541 taataacctc ctacaaatgg ttttgtacaa tctgtactaa ttctacgata taatcgccaa
    12601 tgatgggcaa gtttaaaaca actaaacgtc gcagaagaga gatcacaacg gctgcgacga
    12661 cggtagcccc aatggctgcc ccaacaccac gccccacgcc gcccagaaag ttaacccaaa
    12721 aaaaacgacg ggggttattt aaaaggttca gatactcggc aatgttgaat ttatcaattt
    12781 cctgcagcag acgagcgaca cggtggttaa gtctttttaa taaaaactca tccggctttg
    12841 acggcccttc cacaacgctt ccctcctctt tagatttttc ctcattccct catcgcttac
    12901 agtttcccca aactgcatga tctcaagcaa aaaaatcgtt aaaaaacggg cgaattgtct
    12961 tcgcccgcag tttaatgact acgcagtcaa gttgcttatg attgagctat aacactttct
    13021 gtgcaacgag ggcacaaggt aggatgagtg gcgtcaatgc caagctgagg atgaatcatc
    13081 caacaacgtt cgcatttttc cccggcagct gttttaactg caacgcctaa accttgttca
    13141 actactgttt ctgccgctcc tgcatccggt tcgtggagtg tacaggccga aacaatgaat
    13201 atttccgcca aacgttcctt aaatggcagt aaagcatcat aggctgtttg gtcgggatac
    13261 agctccaggg ctgccgttag ggatgatccc agccgttttt cttgacgtgc ttcttccagg
    13321 cgacgcgtga ccacgtcacg gtaggttaat aaagc
//
LOCUS       DASRMS010000115         1725 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_500917, whole genome shotgun sequence.
ACCESSION   DASRMS010000115 DASRMS010000000
VERSION     DASRMS010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1725)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1725)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1725
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_500917"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1650)
                     /locus_tag="VFC74_05485"
     CDS             complement(52..1650)
                     /locus_tag="VFC74_05485"
                     /inference="COORDINATES: protein motif:HMM:NF013974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HZK24819.1"
                     /translation="MKKQLVLGAEAIAMAALDEGISAVFAYPGTPSTEIAEYIQNDAY
                     AQKEKIHSRWSVNEKTAYESALGMSYAGKRSIVCMKHVGLNVAADAFMNSSITGIHGG
                     LVLVVADDPSMHSSQNEQDSRVYGKFSFLPLLEPSNQQEAYNCVRDAFKLSEKVKLPV
                     ILRITTRLAHSRAIVERMEAVAQNELNPETDKKKWILLPAIARNSYAALLEMQKDLVN
                     YSEASPYNRLVEGSGTKGVIAFGLAFNYVMETLREYELDIPVLKISQYPLPEKMIKEF
                     FNKYDSILVAEEGYPVYEELLRGYLDNPKIAGRLDNALPRKGELTPDAIAEALGVKQA
                     AGEQQDALSVPGRPPRLCDGCGHRDLYEALNKTLLNYSQQHVFSDIGCYTLGALPPFN
                     AINTCVNMGASITMAVGAAQAGLNPAVAVIGDSTFTHSGITGLLDAVNFGLPITIIIS
                     DNDTTAMTGGQDSAGSGSLYDICVGVGVSKEHIKTIVPLKRNLEENIHILKDEMDYNG
                     VSVILASRECVQTLKRNRRRSKNA"
BASE COUNT          499 a          489 c          270 g          467 t
ORIGIN      
        1 cccgcaagcg ttaaaacacc ttgtccgcct acacccgcta gaataacatt tttatgcatt
       61 ttttgatctc cttctatttc gttttaatgt ttgcacacac tcacgtgatg ccaaaattac
      121 tgatacacca ttataatcca tttcatcttt cagaatatga atattctcct ccagatttct
      181 ctttagcgga acaattgttt tgatatgctc tttcgacaca cccacaccca cacatatatc
      241 atacaaacta ccactacccg ccgaatcctg tcccccagtc atagctgtag tatcgttatc
      301 agaaattatt atcgttatag gcaatccaaa attcacagca tccaacagtc ccgttatacc
      361 agagtgcgta aaagtagagt cgccaattac cgccacagcc ggattcaagc cagcctgagc
      421 agcacccaca gccatcgtta tcgatgcacc catattcaca cacgtgttta tggcattaaa
      481 cggtggcaaa gcacccaacg tgtagcaccc aatatccgaa aacacatgct gctgactata
      541 attcaacaac gttttgttca gcgcctcata caaatctcta tgtccacaac catcacacaa
      601 ccttggtgga cgacccggca cacttaaagc atcctgctgc tcaccagctg cctgtttcac
      661 acccaaagcc tcggcaatag catcaggcgt aagctcccct ttacgaggca aagcattatc
      721 caacctaccc gctatttttg gattatcaag ataacccctc agcaactcct catacaccgg
      781 ataaccctct tcagccacca atatagagtc gtatttatta aaaaactcct ttatcatctt
      841 ttcaggaagc ggatattgag atatcttcaa caccggaata tccaactcat actccctcaa
      901 agtctccatc acataattaa aagcaagtcc aaaagcaatc accccctttg tgccactccc
      961 ctccaccaac ctattataag gcgatgcttc agagtagttt accaaatcct tttgcatctc
     1021 cagcaaagcc gcataactat tgcgtgcaat ggcaggaagc aaaatccatt tctttttgtc
     1081 cgtttcaggg ttcaactcat tttgagccac cgcctccatc cgctccacta tagcccttga
     1141 atgagccaat cgagtagtaa ttctcagtat cacaggcagt ttcacctttt ccgacaattt
     1201 aaaagcatca cgcacacagt tgtatgcctc ctgctgattg gaaggttcga gcagcggtaa
     1261 aaaagagaac ttaccataca cccgcgagtc ctgctcgttt tgagacgagt gcatcgatgg
     1321 atcgtcagcc accaccagca ccaatccgcc atgaataccc gttatagacg agttcataaa
     1381 agcatcagca gccacattca agcccacatg tttcatgcaa actatactac gcttgccagc
     1441 atacgacatt cccaaagcac tctcgtaagc agttttttca tttaccgacc acctagagtg
     1501 tatcttctct ttctgtgcat aagcatcgtt ttgtatgtat tcggcaatct ctgtagaggg
     1561 tgtgcccgga taggcaaaca ccgcagaaat accttcatca agagcagcca ttgcaatagc
     1621 ctcagcgccc agaaccaatt gttttttcat aaattaaatt tcttacaagt tattattgtt
     1681 tcacatgttt tagttcgatg tcaacaaggc tttataccct aatac
//
LOCUS       DASRMS010000116         1059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_163186, whole genome shotgun sequence.
ACCESSION   DASRMS010000116 DASRMS010000000
VERSION     DASRMS010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163186"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..416
                     /locus_tag="VFC74_05490"
     CDS             225..416
                     /locus_tag="VFC74_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24820.1"
                     /translation="MNKPTVKQILDKAFKECYEIHGFKIGMVLNTWQDSTTADGRDSA
                     HLTESHVEGIFYDVTEKQK"
     gene            670..1044
                     /locus_tag="VFC74_05495"
     CDS             670..1044
                     /locus_tag="VFC74_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24821.1"
                     /translation="MGRPRTTVDDLPSDWKNILIDCGQEGGSAVEMRCLLGIGESAWG
                     TLLEDSVDFQRTVKEARDLCEVWWERTGRKMATGADGNATVWIFNMKNRFNWRDKIEQ
                     EHTSPDGSMSPTTISLVGPSEG"
BASE COUNT          361 a          208 c          257 g          233 t
ORIGIN      
        1 taattagagc tgctataacg aaaaatcagc agtgttttgt accacaaata aacaatgaaa
       61 ataatggcag ggttagtcat gaggcgattc gtgcggcaac ttccgaaatg acaaagagca
      121 aagagctgcg gcttaaagaa gactcttacg agcatgactg ggaatacata ctgcgctgag
      181 tcataacaaa accattaacg ggctttaaca aaggagcaac aaaaatgaac aaaccaacag
      241 taaagcaaat actagataag gcattcaaag agtgctatga aatacacggc tttaaaatag
      301 gcatggtgtt aaacacatgg caagacagca caaccgcaga tggtcgagac tcagcacacc
      361 taacagagtc gcatgtcgaa ggtatttttt acgatgtaac ggagaaacaa aaatgaacac
      421 aatcaaagca atgcaactaa tgagcagcaa gaagaaaacg agccatatag tacacttgat
      481 aatatgctta ctaactatgg gtttctggct gcctgtttgg gtgctagtag gcgtgtctaa
      541 ttcgctacac aatagcagcg tagaccgtaa gcttaataag ctagctaaac agtgatatac
      601 taacaccacg cccgtcatgt gcgggtttta attttttagg ggcggcttat ggaagaagtt
      661 aaaaacccag tgggtaggcc acgcactacg gtcgatgact taccaagcga ctggaaaaac
      721 attctaattg attgcggaca agagggtggc agtgctgtag aaatgcgctg cttactgggc
      781 attggtgagt ctgcatgggg cactttacta gaggactcag tagattttca acgaaccgta
      841 aaagaagcac gtgatttatg cgaggtctgg tgggagcgga caggtcgaaa aatggcgacc
      901 ggtgcagatg gtaacgcaac cgtttggatt ttcaatatga agaaccgatt taactggcgt
      961 gataaaatcg aacaagaaca cacctctcct gacggtagca tgtcaccaac aacaatctcg
     1021 ctagtaggcc cgagtgaagg ttgaggtaca aataccgcc
//
LOCUS       DASRMS010000117         1311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_231579, whole genome shotgun sequence.
ACCESSION   DASRMS010000117 DASRMS010000000
VERSION     DASRMS010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_231579"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1031
                     /locus_tag="VFC74_05500"
     CDS             234..1031
                     /locus_tag="VFC74_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24822.1"
                     /translation="MQEELLEASEYEAWLKKFETKKTTDSCFTPAAIYSAVLDWAANE
                     YGLKGRRILRPFHPEGDYENVDYQVGDVVIDNPPFSILTKIVRFYESKGVDFFLFAPS
                     LTLFSSNAKCSIVTNSTIEYANGAKINTSFLTSLDGAKVRTAPELKKVIADAEAASKA
                     EKPKLPKYIYPSNAITAALLGKIASVDFKVYPNEISEKLSRLDSQKEAKKTFFGSGFL
                     ISDRKAAELKAAELKAAELKAAEIKATVGAIEWPLSERERKLIKGLA"
     gene            1038..1187
                     /locus_tag="VFC74_05505"
     CDS             1038..1187
                     /locus_tag="VFC74_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24823.1"
                     /translation="MVFNGGKMELDSFELDESALAEAEKRREQKLTDDEQPVAAADDC
                     EACKI"
BASE COUNT          422 a          252 c          328 g          309 t
ORIGIN      
        1 aggtgctggc gcttgtcgat aatgaaggca tacacaagca tattgtcatg gatgcatacg
       61 atcagtacgc tatgcggact ccggagaacg ctgcaaatca gcgtgcagag tttggtgtaa
      121 agtgggcgtt gttctgcatt aaaaacagat agtcgtattg tggtataatc aacccgtcgt
      181 aaatggcggg tttttcatgg tggtagctat ggcagactgg ggaggcgcaa taaatgcaag
      241 aagaactgct ggaagctagc gaatacgaag cttggttaaa aaagttcgaa accaaaaaaa
      301 caaccgatag ctgtttcaca cctgctgcta tttattcggc agtattggat tgggcagcga
      361 atgagtacgg attaaaagga cgccggattt tgcgcccgtt tcaccccgag ggtgactacg
      421 agaacgtaga ctatcaggtt ggagatgttg ttattgataa cccgcctttt tcgatactaa
      481 ccaaaatcgt taggttttac gaaagtaaag gcgttgattt tttcttgttt gcgccatcgt
      541 taactctgtt tagctctaac gctaaatgct cgatagtcac aaactcaact atcgaatacg
      601 ccaatggcgc aaaaattaac acgtcatttt tgactagctt agatggtgca aaagtacgca
      661 ctgcaccaga attaaaaaaa gtgatagcag acgcggaagc ggctagcaaa gccgaaaagc
      721 caaagctgcc aaagtacatt tacccgagca acgctataac agcagctttg ctaggaaaaa
      781 ttgcaagtgt agattttaag gtttatccca acgaaataag cgaaaagcta tcgcgcctag
      841 actcgcagaa agaagcaaaa aagacctttt tcggcagcgg ctttttaatc tcagacagaa
      901 aagcagctga gctaaaagca gctgagctaa aagcagctga gctaaaagca gcagaaatta
      961 aagccaccgt tggcgctatc gaatggcctc tgtccgagag agagcgaaaa ctaattaagg
     1021 ggctagcatg attaacgatg gtttttaatg gtggtaaaat ggaattagac agttttgagt
     1081 tagatgagag cgcactagca gaagctgaga agcgcagaga acagaaactc acagacgatg
     1141 agcagccggt tgctgctgct gatgactgtg aagcttgtaa aatttgaggt ggtggtatgg
     1201 atgaccaagt gaaaagacct ataggcaggc caacaaagta taacgatgag ctacaggctc
     1261 tagcagacac ctatatcttt aactacaaag agcaaggcga tgtgattcct a
//
LOCUS       DASRMS010000118         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_299693, whole genome shotgun sequence.
ACCESSION   DASRMS010000118 DASRMS010000000
VERSION     DASRMS010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_299693"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..695
                     /locus_tag="VFC74_05510"
     CDS             <1..695
                     /locus_tag="VFC74_05510"
                     /inference="COORDINATES: protein motif:HMM:NF024872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK24824.1"
                     /translation="TPEMIVVDTLHRFMHGDENSAQDTKTMLDACAGLMSEFGCSVVL
                     VHHTGVSDEAQHRARGSSAWRGALDIEISVRPGKDNQPIEVSQKKSKDAEMADPLYFD
                     LTSINIEGWFDEEGEPVTTAVLQPADAPVAPKRESASDRRMKRLEAAWVDSGAELDEN
                     GRPNISRSALLNYLTNTCELSESSARQELVPSSGRLIGNLLDNQVIEPTEHGWSVINS
                     VVASAWILSKKA"
     gene            873..>1081
                     /locus_tag="VFC74_05515"
     CDS             873..>1081
                     /locus_tag="VFC74_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24825.1"
                     /translation="MNFTKKEKEKMNEIKIVLPWPDSALMPNRRGGKHWASFQKKKEG
                     ARADGADATRNALEGLRGVSMAEGEV"
BASE COUNT          357 a          189 c          285 g          250 t
ORIGIN      
        1 aaacgccaga aatgattgtg gtggacacgt tgcaccgttt tatgcacggt gatgaaaaca
       61 gcgctcaaga cacaaagaca atgctagacg cttgcgcggg tcttatgtct gagtttggtt
      121 gcagtgtcgt gcttgtacac catacgggcg taagcgatga ggctcaacat agagcgcggg
      181 gttcgagcgc ttggcgtgga gcactagata ttgaaataag cgttagacca ggcaaagaca
      241 atcagcccat agaggttagc caaaagaaaa gtaaagatgc tgagatggcg gatccgcttt
      301 attttgattt aaccagcatc aatatagaag gttggtttga tgaagagggt gagcctgtca
      361 ccaccgctgt gctgcagcca gccgatgccc cagtagcacc aaaaagagaa agcgcatctg
      421 atcgtcgtat gaaaagacta gaagctgcgt gggtcgattc tggtgcggaa ttagatgaaa
      481 atggtaggcc aaatataagc agatcagcac tattaaacta cctaactaat acgtgcgaac
      541 tttcagaaag tagtgctcgc caggagctgg ttccaagttc aggaagattg attggaaacc
      601 tgctagacaa tcaagtaatt gagccgacag aacatggctg gtcagtcatc aattcggtgg
      661 tggcaagtgc atggattttg agcaaaaaag catgaataca tacaccatac aaacatacat
      721 aaacatacaa tgtattaata tgtatacatg tatgaaaagg gggcaaatag ctactaaaac
      781 agcatacata cacatacacc cctctttaga gggtgtatgt atgtatgctt agtatgctgc
      841 gtcagtatgt aggtttagaa aaagtaggtt agatgaattt tacgaaaaag gaaaaagaaa
      901 aaatgaatga aataaaaatt gttttaccgt ggccagattc ggcactgatg ccaaaccgaa
      961 gagggggcaa acattgggct agttttcaaa agaaaaaaga aggggcaagg gctgatggag
     1021 cagatgcgac tagaaatgct ttagagggat tgcgtggtgt ttcaatggct gaaggtgagg
     1081 t
//
LOCUS       DASRMS010000119        10329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_163614, whole genome shotgun sequence.
ACCESSION   DASRMS010000119 DASRMS010000000
VERSION     DASRMS010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 10329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10329
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163614"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="VFC74_05520"
     CDS             <1..853
                     /locus_tag="VFC74_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24826.1"
                     /translation="MSAEDYDTLIEDFPAFRKTMFLRKYPVFNESNAVAYEALKEAAM
                     EYKTYFLANSLINETLFGDKQIVSLHGPESLRYSSAFNSLFDYYRGIPKSLSDLRRRP
                     AVVREACKVIRDRALSQLSAKPEDYQGKGFPLGMTVYHSECFLSPKQFDELFFKDFKE
                     FYGPFMEAGAKFFLKGEGKFLNTIDRYRQLPKGAIVMMLDEDDPFEVYKAIGDWATVA
                     TGIRSDLLKYGTKQQCIDYVKKSFDTFAPGGGFMFLQNKPFLYAQDVKPENVVAAYEF
                     ANEYGKK"
     gene            866..997
                     /locus_tag="VFC74_05525"
     CDS             866..997
                     /locus_tag="VFC74_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24827.1"
                     /translation="MSEQTFGIEELLAQLKQDDSWESDPKLREQIVDALYGSLTRGV"
     gene            1147..3012
                     /locus_tag="VFC74_05530"
     CDS             1147..3012
                     /locus_tag="VFC74_05530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZK24828.1"
                     /translation="MKPTIAIMSYRWLTSLIRKIEGKFIEHVNLTIIDALLEDALAEA
                     QKMEEKQHVDVFVSAGGNARLLAQYLNSPFLEIQVTGFDILLALKKAGQYGDRIGVLT
                     YRHKLPYLAEVTDMLNYDVRELTYNALSNIDIVLEQLQNEGINTVVGASLVLERAQER
                     GMNGVFIYSVEGIERTLESAVKIAYSKKIESEKAERLRVILDFAYSGIIATDKYGKIT
                     AFNPSAAKIMGITRRNVMGRSVSKAIPNTRLMEVLTSAQSELNQIQSIGEIKILTNRV
                     PIMVNGEVTGAVATFQDISAIQKAEETIRARLYKKGFLAKATVEDVLGDSVVMRQVKR
                     KACLYAKTDSTVLIFGESGTGKELFAQGIHNASTRSKRPFIAVNCAALPENLLESELF
                     GYEDGAFTGARKGGKAGYFELAHGGTIFLDEIGEISEKLQARLLRVLEEREVMRIGGD
                     RIIPVNIRVVAATNRKLWQLVEKGKFRQDLYYRINVLELEIPPLRHRKEDIQLLGGYF
                     LANFRRELERGEIAEIMSYPQFQSYHWPGNVRELKNVMERLAVLYENGTDYKELLATF
                     FNKQNAVTLLNSEEEEIEKVLQEVGGNKSEAAKRLGISRTSLWRKLKKYETHRNN"
     gene            3435..4697
                     /gene="leuC"
                     /locus_tag="VFC74_05535"
     CDS             3435..4697
                     /gene="leuC"
                     /locus_tag="VFC74_05535"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HZK24829.1"
                     /translation="MGMTITEKILAHNSGQEQIEAGEIVNAKLDGVLANDITAALAIP
                     QLQEMGYEKVFDAERVFFVMDHFVPNSNVNAAMQCKVCRDFARASDLKYFYDVGEMGI
                     EHVLLPEKGHVLPGDVLIGGDSHTCTYGALGAFATGVGSTDLAAGLAKGELWFKVPPS
                     LKFIYEGKLPRYVSGKDLILYTIGQIGVDGALYKAMEFAGAAITELPMDGRFTMCNMA
                     IEAGAKNGIIAPDEITEKYVRMRARRPCQIYTSDADANYEKVYTFDVSALEPQVAVPH
                     LPSNARNISEVKSITLDQVIIGSCTNGRLSDLRAAAALLTGQKVHPYVRTIIIPGSQE
                     VYLQALKEGLIEIFISAGAAVSTPTCGPCFGGHVGVLAAGEVCLSTTNRNFVGRMGHP
                     QSAVYLASPVVAAASAIKGRVASPWEVV"
     gene            4697..5179
                     /locus_tag="VFC74_05540"
     CDS             4697..5179
                     /locus_tag="VFC74_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015516588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HZK24830.1"
                     /translation="MGKAFVFGDNIDTDLIVPGKYLTLSTPAELATVCMAGLAEDFVL
                     RISEGDIFVAGRNFGCGSSREHAPVSIKAAGVSCVIATSFARIFYRNAINIGLPVLES
                     AGAAAGIREGDEVLVSMEKGLIINQSTDESYTFTAFPKFILEIFQAGGLVDFIKMQHK
                     "
     gene            5194..6048
                     /locus_tag="VFC74_05545"
     CDS             5194..6048
                     /locus_tag="VFC74_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014345227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HZK24831.1"
                     /translation="MEKTTLRDLLNKPGIIRAPGAFDAWSARLVQMAGFPAIYMTGYG
                     VSASALGSPDIGLITMNEMVNQAKNMSAAGIPLIADADTGYGGVLNVMRTVKEYERAG
                     VAAIQLEDQVLPKRCGHMEGKELVTQEEMVSKVRAAVAARASEDTLIIARTDARAVHG
                     FEDALKRAAAYQAAGADIIFFEAPQSVEEMKTVAATLHVPLLANMVEGGKTPFLTATE
                     LEEMGYKLVIYPASTLYAATKSMISVLQSLKEHDTSAQYREQMVGFPEFYRLIGVEAA
                     RQLEKSFS"
     gene            6078..7154
                     /locus_tag="VFC74_05550"
     CDS             6078..7154
                     /locus_tag="VFC74_05550"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HZK24832.1"
                     /translation="MKRKALIIFLCLTLVLVTLTGCGSTDTTPGTNEGNDKETFHVSI
                     AAGRPGDMWYVLSHALATFINERSDWVTAEVVATAGVTDNTRLVMGEKELRGTHLNVT
                     MLPGATVWGEGEYLPLQIGMLVMLNETFVTLDPNIETLADFSNKTVVLPRDAPDAYGW
                     IFRNMLDLAGAKNYTLMHGGIDARLTALRDGAAEVGVLPFDFYYPNEFALSSGLMELG
                     ARGTLYFPNQGNVRQNLDVVTNACVADPFTGEKELPPLGMVAPAGSMGETQTEEMVYV
                     STPVYWSAGEEMPEEVVYEITRILYEAAENGDFEPYHAMGKGITTEFLAMSFWSDEQR
                     EAMFHPGALKLYQEKGVALKSFLD"
     gene            7250..9163
                     /locus_tag="VFC74_05555"
     CDS             7250..9163
                     /locus_tag="VFC74_05555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter fused permease subunit"
                     /protein_id="HZK24833.1"
                     /translation="MEEQVKQNTTPSKWLDYIFYALVVLFIGYQLYYIRTLPFEPTVH
                     AILHLGFALALSAWGKLRQGASKPITRAFIALVFFASIAVTLYFYFSYKSILANPSYP
                     PMIALIFGVIASLVVIIVVSWNYGWLFPIFAVLAAIYVQYGKFIPGMFRAPSTTWQRT
                     ITLLATDVTSPWGLYGNLVGLSANYLFLFIIFGSVLHAFGGLRFIVQLGNLVASKLRS
                     GPAALAVVSSALLGSITGSTAANVTITGSFTIPLMKQAGYTPDQAAAVEAAASNGGQF
                     LPPVMGATVFVLSAYTGIPYLEIAKAAVVPALIYFFMLLLYAELNARKLNITTVPSQL
                     DWRQLLLDAPLFIMPLATLVVMLAKGYSLMMVVFWSTVCVVVLGLVSGLRQDARIDWA
                     DVREKFAEGAMTGANVATILAVIGIAVAALEVTGLTLKLSVVLGNLAGNNIFLLLFFT
                     MLASLILGTGVPTPAAYMIVATVLSPLLIRQGLPVMQAHLFPMFYAFLSHLTPPVGIG
                     LLIATKLAGSEYWRGAREVFKAAYPSLFLPYLFVYVPELLLQYNDIGTWTLKVLAVTL
                     FFFTTAILLNKQWKNKVLVGEQVLLLLSSVLIVVYLFISTHPLSLFLAVLFALIPLII
                     NSRRSTVAIIQTK"
     gene            9358..10137
                     /locus_tag="VFC74_05560"
     CDS             9358..10137
                     /locus_tag="VFC74_05560"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZK24834.1"
                     /translation="MSCQDKSIKGLPADCMPQQECLDAMQIYRLTLETILKAMHKMLA
                     SLPQAEVPGATCRIPTSCPAGFTGRYTVQPGDSMFLIAQRCGVSLQSLINANPQITDP
                     SQIFPCDVLCVPLQCANQDSLPGSTTCRIPGACPVGFTGRYTVQPGDSMFLIAQRCGI
                     SLQSLINANPQITDPGQLVPCDVLCVPLQCANQGTCRMPTSCPAGFPGRYTVQPGDSM
                     FLIAQRCGISLQSLINANPQITDPGQLVPCDVLCVPPQCAN"
BASE COUNT         2873 a         1982 c         2495 g         2979 t
ORIGIN      
        1 tatgtcggca gaggattatg atactttgat tgaagatttt cctgctttcc gcaaaacaat
       61 gtttttgaga aagtatccag tttttaatga atctaatgca gtcgcatatg aggcattaaa
      121 agaagcagcg atggaatata aaacttattt ccttgctaat tcattgatta atgaaactct
      181 ttttggggat aaacagattg tctccctgca cgggccggaa tcacttagat attcctccgc
      241 ctttaactca ctttttgatt actatagagg gattcccaag tccttatccg acttaagaag
      301 gagaccagct gtagtcagag aagcttgcaa ggtcatcaga gatcgggcat taagccagct
      361 tagcgctaaa cctgaagatt atcagggcaa aggttttccg ctaggcatga ctgtttatca
      421 tagtgaatgt ttcctttcgc ccaaacaatt tgatgagttg ttcttcaagg acttcaagga
      481 attttatggc ccctttatgg aggcaggcgc aaaattcttc ctcaagggtg aaggtaaatt
      541 ccttaatact attgaccgct atcggcagct gcccaaaggg gcaatcgtga tgatgctaga
      601 tgaagacgat ccctttgaag tatataaggc aattggtgat tgggcaaccg tggccaccgg
      661 tattcgtagt gatctgctga aatacggcac aaaacaacaa tgtatagatt acgttaaaaa
      721 atcgtttgat actttcgccc ctggcggtgg cttcatgttc ctgcaaaaca aacctttcct
      781 atatgctcaa gacgtaaagc cggaaaatgt agtggcggcc tatgaatttg ccaatgaata
      841 cgggaagaaa taggaggcat gaagaatgag tgaacaaacc ttcggcatag aagaattatt
      901 ggcgcaatta aagcaggatg attcctggga aagcgatccc aaattaagag aacagattgt
      961 cgacgcgctg tatggttcac taactagagg cgtctagcaa ttggcagtaa ttacagccca
     1021 agccggttta ggcttgggct gttcagtcac ttgttttaca aaaaatacaa gtagtacctt
     1081 catttgtgta aaggggttta agttgacaaa gagaagtatt ttttaatagc aactggaggg
     1141 atgtatatga aaccaacaat cgccattatg agctaccgct ggttgacttc acttatacgt
     1201 aagatcgaag gtaaatttat tgagcatgta aatttaacaa ttattgatgc tttactggaa
     1261 gatgccttag ctgaagcaca aaaaatggag gaaaagcaac atgttgatgt ttttgtttca
     1321 gctgggggta atgcccgttt gttggcacag taccttaata gtcccttctt agaaattcaa
     1381 gttactggtt ttgatatctt attagcttta aaaaaagcag gtcaatacgg agatagaatt
     1441 ggtgtgttaa cctaccggca caaactgcct tacctggcag aagtaactga tatgctgaat
     1501 tatgatgtga gagagttaac ctataatgct ttgtccaata tcgatatagt tttagaacaa
     1561 ctgcagaatg aaggtataaa caccgttgtg ggtgccagtc tggtgctgga aagagcacag
     1621 gaacgaggca tgaatggtgt gtttatatat tcagttgagg gcattgagag aacattagag
     1681 tctgctgtaa aaattgctta ctccaaaaag atagaatctg aaaaggcaga acgtttgcgc
     1741 gttattttgg attttgccta tagcggtatt atcgccacgg ataaatatgg caaaattact
     1801 gcctttaacc ccagtgccgc aaaaataatg ggtatcacca ggcgaaatgt gatggggcgt
     1861 tccgttagta aagctattcc taataccaga ttaatggagg tgctgactag cgcccagtcg
     1921 gaattaaatc aaatacaatc tattggggaa ataaaaatcc tcaccaatcg cgttccaatc
     1981 atggttaacg gtgaggtgac gggtgccgtc gccacattcc aagatattag tgctattcaa
     2041 aaagctgaag aaactattag ggcgagatta tataagaagg gttttctggc taaagccact
     2101 gttgaagatg ttttaggtga cagtgtcgtg atgcggcaag taaagcgcaa ggcgtgtctt
     2161 tatgctaaaa ctgattctac tgtgttaata tttggtgaat caggtacagg taaagaattg
     2221 tttgcgcagg gaattcataa tgctagcacg cgctctaaac ggccatttat tgctgttaac
     2281 tgtgctgctc tgccagaaaa cttgttggaa agtgaacttt ttggctacga ggacggcgct
     2341 tttactggtg ccagaaaagg cggtaaagct ggctattttg aattagctca tggaggaaca
     2401 atatttcttg acgagattgg tgagatctct gaaaaattac aagctcgttt actgcgcgta
     2461 ctggaagaac gcgaagttat gcggattggc ggggatcgta tcatacctgt taatatccgc
     2521 gtcgtggcag cgacaaatag aaagttgtgg cagctggtgg aaaagggtaa atttaggcaa
     2581 gatctatact atcgcattaa tgtcttagaa ttggagattc caccgctacg ccacagaaaa
     2641 gaagatattc aacttttagg tggctacttt ttagctaact tccgccgcga acttgagcgg
     2701 ggtgaaattg cagagataat gtcctatcct cagtttcagt cttatcattg gccgggtaat
     2761 gtacgtgagt taaagaatgt tatggaaaga ctcgctgtac tttatgaaaa tggtacggac
     2821 tataaagaac tgctagcaac cttttttaat aagcagaatg cagtaacttt gctaaatagc
     2881 gaggaagaag aaatagaaaa agtattacaa gaggtgggcg gaaacaaatc agaagctgcc
     2941 aaaagattag gtatcagccg caccagtctc tggcgcaaat taaagaagta tgaaacacat
     3001 agaaacaact gaaacactat actgtaacac tgaaacgttt ttactacatg cgctttactc
     3061 ttgtttagag gaaaagcgca tttttattaa ccaatattta ctatctttgc aggttaccag
     3121 tggcgaaaag cgctggatga aggaaggcaa ggggcatgca tttcttgttg gcaagggaaa
     3181 tattaacttc ttcatgcctt ttctcttcta taacggaggt aattaaatgg ggactttatt
     3241 tattttgcgg tgtggaaagt gtcgtaaagt cgttgccggc acagcagcag attccttgtt
     3301 ttgactgtga gatcggacgt gctttaggga gtttaaaagt tagatattta tgcttgttgt
     3361 ttaatcttgg catgactttt gcactgatat taggtaatta agtaccaatt gtctgaggaa
     3421 gaggggtgtt gcatatgggt atgacaatta cggaaaaaat tttagcccat aatagcgggc
     3481 aggagcaaat tgaggctgga gagatagtca atgctaaatt agacggtgtt ttggccaatg
     3541 atatcacagc agcgctagct attccacaat tacaggaaat gggttatgag aaagtatttg
     3601 atgcagagag agtgtttttc gtgatggatc attttgtacc gaatagcaat gttaatgcag
     3661 ccatgcagtg taaggtctgc cgcgattttg cccgtgccag cgatctgaaa tatttttatg
     3721 atgtaggtga gatgggtatc gagcatgttc ttttgccaga aaaaggccat gttttaccgg
     3781 gtgatgtatt aatcggtgga gattcccata catgcactta tggtgcactg ggcgcctttg
     3841 ccactggcgt gggcagtact gatttagcag caggtttagc aaaaggtgaa ttgtggttta
     3901 aagtacctcc cagtctcaaa tttatttatg aaggaaaatt accaagatat gtgtctggta
     3961 aggatctaat tctttataca attggtcaga tcggtgtcga cggtgctttg tataaagcaa
     4021 tggagtttgc aggtgcggcc attacagagc tacctatgga cggacgcttc accatgtgca
     4081 atatggcgat tgaggcagga gcaaagaatg gaattattgc ccctgatgaa ataacagaaa
     4141 aatatgttag aatgcgggcg cgacgacctt gtcaaattta cacaagcgat gcggatgcca
     4201 attacgagaa agtttatact tttgacgtct ccgcattgga gcctcaagtt gctgtgcctc
     4261 atcttccctc taatgcaaga aatatcagtg aagttaagag cataacactg gaccaagtca
     4321 tcattgggtc gtgcactaat ggtcgactaa gcgatcttcg tgcagcagca gctctcctca
     4381 caggccagaa agtgcacccc tatgtgcgca cgattataat ccctggttct caggaagttt
     4441 atttgcaagc cttaaaggaa gggttaatag aaatatttat ttcagccggg gctgctgttt
     4501 ctacgccaac ttgtggtccc tgttttggtg ggcatgtggg agttttagca gcaggtgaag
     4561 tttgtctatc cactactaac cgtaattttg taggtcgcat ggggcatcca caatcagccg
     4621 tttatttagc cagccccgtt gtagctgctg catcagcaat taagggtaga gtcgccagcc
     4681 catgggaggt agtgtaatgg gaaaagcttt tgtttttgga gacaatattg acacagattt
     4741 aattgtgcca ggcaaatatt tgacactttc tactcccgcg gagttggcga cagtctgtat
     4801 ggcgggtctg gcagaggatt ttgtcttacg gattagtgag ggagatatct ttgtggcggg
     4861 gagaaacttc ggctgtggca gctctagaga acatgcgcca gtaagcatca aagcagccgg
     4921 agtcagctgc gttattgcta caagttttgc caggatattt taccgtaatg ctattaatat
     4981 tggtttgccg gttttagaat ctgcgggggc ggctgcagga atcagggaag gagatgaggt
     5041 gttagtcagc atggaaaagg gtttgattat taatcaaagc acagacgaaa gttatacttt
     5101 taccgccttt cctaagttta tcctagaaat ttttcaggca ggaggtctag tggattttat
     5161 taaaatgcag cataaataga ggaggaagag ttagtggaaa aaactacatt acgtgatttg
     5221 ttgaacaaac caggaataat tcgtgcccca ggagcatttg atgcttggtc ggcaaggctt
     5281 gtgcagatgg cagggtttcc cgcaatttat atgacaggtt atggtgtgtc ggcaagcgct
     5341 ttaggatctc cggatattgg cttaattacc atgaacgaaa tggtaaatca ggcgaaaaac
     5401 atgtctgcag ctggcatccc acttatagct gatgccgata caggctatgg tggtgtttta
     5461 aatgtaatgc gcactgttaa ggagtatgag cgggcaggag tagcagcgat ccaattggag
     5521 gatcaggtgc tgccaaaacg atgcggtcat atggaaggaa aagagcttgt tacacaggag
     5581 gaaatggtgt ccaaagttag ggcagctgta gctgcaagag catcggaaga taccttaatt
     5641 attgctcgaa cggatgccag agcagttcat ggatttgaag acgcactaaa gcgtgcggca
     5701 gcttaccagg cagcaggtgc tgatataatc ttctttgagg caccacaatc agttgaagaa
     5761 atgaaaacag tagctgctac attgcatgtg cccttattag cgaatatggt ggaaggcggc
     5821 aaaactccat tcctgacagc aacagaactg gaagagatgg gttataaatt agtaatttat
     5881 cctgcctcaa cgctatatgc tgctacaaaa tcaatgatca gtgtcttgca gagccttaaa
     5941 gagcatgata cctcagcgca ataccgtgaa cagatggtgg gctttcccga attttatcgt
     6001 ttaataggtg tagaagctgc tcgtcaatta gaaaaatcat tttcctaggt aaaaatacca
     6061 attaggagga gagaagtatg aagagaaaag ctttaatcat ttttctatgt ctgacactgg
     6121 tactcgtcac cttgaccggt tgtggttcaa ctgatacaac gcctggtact aatgagggga
     6181 atgacaaaga aacatttcat gtcagtatag ctgctggccg ccctggggat atgtggtatg
     6241 tcctttcaca cgctttagcc accttcatta atgaacgttc agactgggtg acagcagaag
     6301 ttgtggctac ggccggtgta actgataata cccgtttggt aatgggtgaa aaagagttac
     6361 ggggtacaca ccttaatgtc accatgctgc cgggagcaac tgtctggggt gaaggtgagt
     6421 atctgcccct acaaattggt atgttggtaa tgttaaacga aacctttgtt actctagatc
     6481 ctaatattga aactttagct gattttagta ataaaacagt agtgctgccg cgagatgcgc
     6541 cagatgcata cggctggatt ttccgcaata tgctggactt ggctggcgct aaaaactata
     6601 cactgatgca tggcggtatt gatgctcgtc tgacggctct acgcgatggt gcagcagaag
     6661 ttggtgtgct tccctttgat ttctattatc cgaatgagtt tgcgctaagt tctggtttga
     6721 tggaactagg tgcacgtggc actctatatt tcccaaacca agggaacgtg cgtcagaatc
     6781 tagatgttgt tactaacgcc tgtgttgcag atccctttac tggtgaaaaa gaacttcctc
     6841 cactaggtat ggttgctccc gctgggtcca tgggtgaaac tcaaacagaa gagatggtgt
     6901 atgtgagtac accggtttat tggtcagcag gtgaagaaat gccggaagaa gtggtttatg
     6961 aaataacacg gattctttat gaagcagcag aaaacggcga ctttgagcca tatcatgcga
     7021 tgggtaaagg tataaccaca gaatttttag cgatgtcctt ctggtctgat gaacagcgcg
     7081 aagcaatgtt ccatcccggt gccttaaagc tctatcaaga aaaaggtgta gccttaaaat
     7141 ctttccttga ttaaaacgtt actactgagg cgggcaagga aatgcaggca atacttgccc
     7201 gcctaaactt taggttacta acagtgcact aatacaggag ggattaacag tggaagaaca
     7261 ggtaaaacaa aataccacgc catcaaaatg gcttgactat atattttatg ctttagtggt
     7321 attatttatt ggttaccaat tgtactacat cagaacactt ccctttgaac caacagtgca
     7381 cgcgattctt caccttggtt ttgcgttggc cttaagtgcc tggggtaaat tacgtcaagg
     7441 tgccagtaaa ccgataacgc gtgcctttat tgctttggtt ttttttgctt ccattgccgt
     7501 aacactatat ttttatttta gctataaatc tattcttgcc aatcctagct atccaccgat
     7561 gattgcttta atctttggtg taattgcctc tttggtggta attattgtgg taagttggaa
     7621 ttatggttgg ttgtttccca tctttgctgt tcttgccgct atatatgtac agtatggtaa
     7681 gtttataccg gggatgttta gagcacctag caccacctgg cagcgtacta tcaccctttt
     7741 agctacggat gtaacttcgc cgtgggggct ttatgggaac ttggtggggt tatcagctaa
     7801 ctatctattt ctgtttataa tctttggttc agttttacat gccttcggcg gtttacgctt
     7861 tattgtgcaa ctaggtaatc tcgtggcttc caaactgcgt agtggccctg cagcattggc
     7921 tgtcgttagc agtgctctgt taggttcaat tactggtagt acagcggcaa atgtgacgat
     7981 tacaggctcc tttactattc ccctcatgaa gcaagcaggt tatacaccag atcaggcagc
     8041 tgctgtagaa gcagcggcat ctaatggcgg acagttttta cctcccgtca tgggggctac
     8101 tgtttttgtt ttgtcagcct atacgggaat accatatctt gaaattgcca aagcagctgt
     8161 tgtgcccgcc ttaatctact tttttatgtt actgttatat gcagagctga atgcacgcaa
     8221 actaaatatc actactgtac ccagccagct tgattggcgg cagcttctat tagatgctcc
     8281 cctctttatc atgcctttag caacacttgt agtaatgttg gcaaagggat attccttaat
     8341 gatggtggtc ttttggagta cagtttgtgt agtcgtcttg ggtcttgtat ctggtttacg
     8401 tcaagatgcc cgtattgact gggcagatgt aagagaaaag tttgctgaag gagctatgac
     8461 aggagccaat gtagcaacaa ttctggctgt tattggtatt gcggtcgcgg ctctggaagt
     8521 gacggggctg acactaaaat taagtgttgt cttaggtaac cttgcaggga ataacatttt
     8581 cctactgctg ttctttacca tgctggccag tctgatatta ggtactggtg tgcctacgcc
     8641 tgcggcgtat atgattgtag caacagtatt atcccctctt ttaatcaggc agggcttgcc
     8701 ggtaatgcaa gcacatttat ttcccatgtt ttatgccttt ctatctcacc tcacaccacc
     8761 agtgggtata ggtttactaa ttgctactaa actggctggc agtgaatact ggcgtggcgc
     8821 cagggaggtt tttaaagctg cctatccctc tttattttta ccgtacctct ttgtttatgt
     8881 accggagcta ttgctgcaat ataacgacat aggcacgtgg acattaaaag tattggcagt
     8941 tactctattt ttctttacaa cagcaatact tctgaacaaa caatggaaaa ataaagtgtt
     9001 agtaggagag caagttttac tattgctatc tagtgtattg atagtcgttt atcttttcat
     9061 tagtacccat cccctgtccc ttttcttagc agttcttttc gctttgatcc ccctaattat
     9121 aaatagtagg cgcagtactg tagcgattat tcaaacaaaa taaaaattat ttgactagat
     9181 agagcgtaat aactaattaa acaacaaaaa gagcggtaca gaaccgctct ttttgttttg
     9241 ccaaccttgc agtatggtct agggaacatt tttttaggca gatcataaga tatataaact
     9301 gaaagaaata agtacagtaa gtaggttacc caaacttata gggaaagagg agaaaaaatg
     9361 agttgtcaag ataaaagtat aaaaggtctg ccagcagatt gtatgcctca gcaagagtgt
     9421 cttgacgcta tgcagattta tagacttacc ttggaaacca tactaaaagc gatgcataaa
     9481 atgctagcat ctctacctca ggcagaagtg cctggggcaa cgtgccgcat acccacatct
     9541 tgtccggcag gttttacagg caggtataca gtgcagcccg gggattccat gttcttaatt
     9601 gcccagcgct gcggggtctc cttgcagtcg ttaattaatg ccaacccgca gattacagac
     9661 cccagtcaaa tattcccgtg tgatgtattg tgtgtaccct tgcagtgcgc taatcaggat
     9721 agtctgccag gctcaacaac ttgccgcata cctggagctt gtccggtagg ttttacaggc
     9781 aggtatacag tgcagcccgg agattccatg tttttaattg cccagcgctg cggcatctcc
     9841 ttgcagtcat taattaatgc taacccccag attacggatc cgggtcaact tgtcccttgt
     9901 gacgtactgt gtgtacccct ccagtgtgcg aatcaaggaa catgtcgcat gcccacatct
     9961 tgtccggcag gctttccagg cagatataca gtgcagcccg gagattccat gttcttaatt
    10021 gcccagcgct gcggcatctc cttgcagtca ttaattaatg ctaacccgca gattacggat
    10081 ccgggtcagc ttgtcccctg tgacgttctg tgtgtgccac cccagtgtgc taattaatta
    10141 cagatactac tcatttaagg tggcctctcc ttatgctgga aaggccacct ttttggtgtg
    10201 cccggcatgg gcggtagctt ggcggtgaaa gtccgctatg ggctttgcag tgggaaccat
    10261 tagccaaagg caagggtgtc catcgtgagg tggaatctga aggaagccag aggcaaagtc
    10321 ccgacccga
//
LOCUS       DASRMS010000120         7540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_365992, whole genome shotgun sequence.
ACCESSION   DASRMS010000120 DASRMS010000000
VERSION     DASRMS010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 7540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7540
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365992"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..601
                     /locus_tag="VFC74_05565"
     CDS             218..601
                     /locus_tag="VFC74_05565"
                     /inference="COORDINATES: protein motif:HMM:NF016187.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HZK24835.1"
                     /translation="MLDKLLYGLEISLIGFTGVMLILYLLYLILLAFSKYFSRGDSKQ
                     KKATCPTVQLSLDKVKESNNNTAPEVVAAITAAIAMYLNSSAEQFNIVSIKPEKGSSS
                     WVLSGRQIIMANRQNLALSRREKTS"
     gene            598..972
                     /locus_tag="VFC74_05570"
     CDS             598..972
                     /locus_tag="VFC74_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016320647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZK24836.1"
                     /translation="MKEFRITVNGQVYQVSVEEVAGTNSPAAKIVTTLATAPAATPPT
                     TTVPQAQAAAGNSITAPMPGAVLEIVVSEGQDVQAGEVVLILEAMKMENEITAPAAGK
                     IKKIVTSKGAAVNTGDLLLVIE"
     gene            983..2131
                     /locus_tag="VFC74_05575"
     CDS             983..2131
                     /locus_tag="VFC74_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018592363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HZK24837.1"
                     /translation="MLDKVFSFVQETGFYAMSLEQLVMIFIACALLYAGIVKKYEPLL
                     MIPISFGILLANLPLGGVMDLPSYDQAGNLVTAGGLIHYFYQGIKLGIFPPLIFLGIG
                     AMTDFGPLIANPLTLLLGAAAQFGIFTTFMGAILLGFPAVQAAAIAIIGGADGPTAIY
                     LASRLAPELLPSIAIAAYSYMALVPLIQPPIMRALTTQKEREVVMEQLRPVSKTEKII
                     FPIVVVIITGLLLPSSIPLLGMLMFGNLLRESGLTDRLSQTAQNDLNNIIVILLGTAV
                     GASAKAETFLSSQTLLILVLGLVAFAVGTAAGVLMGKLLYKLTGGKVNPLIGSAGVSA
                     VPMAARVSQSEGRKANPNNYLLMHAMGPNVAGVIGSAIAAGILLLLLG"
     gene            2366..3763
                     /locus_tag="VFC74_05580"
     CDS             2366..3763
                     /locus_tag="VFC74_05580"
                     /inference="COORDINATES: protein motif:HMM:NF016202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HZK24838.1"
                     /translation="MSLVKKRVPRWTIIIISLLSLLFSCSFIDYLYHRERIYAGVFVA
                     QQDLGGKTTEEAGQLLAEIIDVGEQSVEFIYDELAQTKKFNELGIALDLDSTINVALR
                     TGRQNLHIFRYPERIRLASKSLQLVPQFKIDQTSFETSLQEIFTAIQLEPVDARLKLS
                     ADRKSVNIEPDEPGRMLDVLAAYRALETALQSTYIDLQLNLPIEPVEAEITSASLEAL
                     QVTEPVASFSTAISGNANRLHNIRQAAAAIDQTLIPPGAVFSFNEIVGQTTAATGYRA
                     APIIMNGKIVDGIGGGICQVSSTLYNAVLLSDLQLVERRNHGLRVKYLPPGLDATVAY
                     GSIDLKFKNTRAHALWLRIFLDGNKLTTTIYGTQIPGQEVKVYTTDVQVIPAGEKIIK
                     TAELPKGKRELVTVGQPGYRATVWRSVYLNGQEEKTEKISQDSYKAMPNEYRVGTKEL
                     PVSGEPSEQSETENE"
     gene            3842..5020
                     /locus_tag="VFC74_05585"
     CDS             3842..5020
                     /locus_tag="VFC74_05585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HZK24839.1"
                     /translation="MMRAIIHVDMNAFFASCHQAENPAWRGKPLLVAGDPKKRHGIIL
                     TASYEARQCGIKTAMTVWQAKKLCPQGIFIPPDHHLYLAYSEKILTIFRSYTPLVEPF
                     SIDEAWLDVTGSERLFGSPEEIGQQLQQRILAELEISCSVGIAPNKFLAKMASERQKP
                     RGFTIIGPQDIRTVLWPLPVEKMVGVGRKIAPALKEMGVETIGQLAKMPERLLVSRFG
                     VMGEILRGLANGDDDSPVDPQALDTAKSIGHSITLPRDINDPEDVARILLELSERVGR
                     RLRQGGYSGRTITLTVKDQNFVTITRASTLQTPAYLTETIYQTAQEIYHSRFEPWRKV
                     RLLGIAISNLQARGEGLQLTLWDQTGERLTSLTETADKIKDRFGDYALQRASLYKNKD
                     "
     gene            complement(5022..5435)
                     /locus_tag="VFC74_05590"
     CDS             complement(5022..5435)
                     /locus_tag="VFC74_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HutP family protein"
                     /protein_id="HZK24840.1"
                     /translation="MNQLDSKLIASAAIRLALSADREAEKELKKELYAEGIRSTAVDY
                     GGEYLTAIQKVLERAVVAAKREGVIESTHHEEGGVAGAAREAIAQIMPKAMGLNVGGK
                     VGIARKGDHVAVAIFFAIGLLHLNEVAIGLGHRAI"
     gene            complement(5604..5867)
                     /locus_tag="VFC74_05595"
     CDS             complement(5604..5867)
                     /locus_tag="VFC74_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HZK24841.1"
                     /translation="MLQAVYVADWFFKILNGMLFVRVILSWLPHNPQQPLIRLLYEGT
                     EPLLAPFRRLIPRTALPIDLSPILAYLVLGIVRNLVISTLLRF"
     gene            complement(5957..6232)
                     /locus_tag="VFC74_05600"
     CDS             complement(5957..6232)
                     /locus_tag="VFC74_05600"
                     /inference="COORDINATES: protein motif:HMM:NF023251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HZK24842.1"
                     /translation="MDNYYVATFYSTSQALRFEKTVLKQELAVRMIPVPRVISSSCGL
                     AARIEDAVWPAIAQLLSSEAANAEEVYLFTQLGKTTQAERIWVDERN"
     gene            complement(6242..6451)
                     /locus_tag="VFC74_05605"
     CDS             complement(6242..6451)
                     /locus_tag="VFC74_05605"
                     /inference="COORDINATES: protein motif:HMM:NF013379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HZK24843.1"
                     /translation="MVYKLDVRGLSCPEPVINTKRALAEHDGTVVVVADSFVAAENIR
                     RLVSSLAYQITTEEEHGEFTITITK"
     gene            complement(6441..7520)
                     /gene="yedE"
                     /locus_tag="VFC74_05610"
     CDS             complement(6441..7520)
                     /gene="yedE"
                     /locus_tag="VFC74_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YedE family putative selenium transporter"
                     /protein_id="HZK24844.1"
                     /translation="MERRKIIVAGGVLGGLGVLLAYFGNPVNMGICVACFIRDIAGGL
                     GLHRAAPVQYIRPEIIGFILGAFLIALKNGEFRARGGSSPLIRFFMGVAMMIGALVFL
                     GCPLRMVLRLAAGDLNALVGLGGFIFGIFIGVQFLKKGFSLGRSQKLPTTNGLVIPAF
                     ALILLILLVLAPGFIYFSESGPGAAHAPILLALGAGLLVGVLVQRTRLCQAGCFRDLM
                     LIRDPHLLYGTIAIFVAALVLNIAFGYFKLGFLEQPVAHTEALWNFIGLAVVGLGATL
                     LGGCPLRQVILAGEGDTDAAMVFLGLLAGAAVAHNFGLAASGTGVPVAGKIGVLVAAS
                     FLVIIGFAFSLPLPVSKKGGNTLGL"
BASE COUNT         2189 a         1663 c         1689 g         1999 t
ORIGIN      
        1 gctcaaatat taacactttt aagtaaaaag cgaataacat taaatatatt tcacttttca
       61 agtgagaaag agtatgttaa aatgaggatt ataactatgg ttgcagctag ttcaccccag
      121 ggaggtgcca gcgaaagcga acgcttatag gcacttttat cgagtgtgat gggtattgtc
      181 accagaatga tgtagatgcg aggaggttga tgagttcttg ttagataaat tactgtatgg
      241 tctggaaatc tcactgatcg ggtttactgg tgtgatgctg atcctttatc ttctttacct
      301 aatactcctg gcttttagca aatatttttc caggggagat agtaaacaaa aaaaagcaac
      361 ctgtcctacg gttcagcttt cactcgacaa agttaaagag tcaaataata atactgctcc
      421 ggaagttgtg gctgctatta ctgcggctat agcgatgtat ttgaattcat ctgcagagca
      481 gtttaatatt gtttctatta agccggaaaa aggcagctca agttgggttc tgtcaggaag
      541 acaaataata atggctaaca gacagaattt agcgctgtct cgaagggaga aaacatcgtg
      601 aaagaatttc gtattaccgt caatggacaa gtttatcaag ttagcgtcga agaagtggca
      661 ggaaccaact ctcctgctgc aaaaatagtt accaccctcg ctacagcccc agcagcaact
      721 ccgccaacaa caactgtgcc tcaagcgcag gcggcagcag gaaatagtat tacagcacca
      781 atgcccgggg ccgttctcga aatagttgtt agcgaaggac aggatgtgca agcaggagag
      841 gtagtgctga ttttagaggc catgaaaatg gaaaatgaaa ttactgcacc tgcagcagga
      901 aaaataaaaa aaatagtaac tagtaaaggt gcagctgtaa ataccggtga tcttttactt
      961 gtcatcgagt agggggataa ctatgttgga taaagtattc tccttcgtgc aagaaactgg
     1021 tttttatgcg atgtcgttag agcagctggt gatgattttt atcgcctgtg ccctgcttta
     1081 tgccggaata gttaagaaat atgaaccact cttaatgatt cctattagtt ttggtatcct
     1141 tttagctaac ctgccgctgg gcggggtgat ggatctgccc agctatgatc aagctggtaa
     1201 tttagtaact gctggtggtc tgatccatta tttttaccag ggcataaaat taggcatatt
     1261 tccaccgtta atctttctcg gtattggcgc aatgaccgat ttcggacctt tgattgccaa
     1321 tcctttgact ttgctcttag gtgcggcagc gcaatttggc atctttacta cctttatggg
     1381 tgcaattttg ttaggctttc cggcagttca agcggcagca atcgccatta ttggcggtgc
     1441 agatggcccc acagccatct atttagcgtc acgcttggct cccgaattat tgccgtcgat
     1501 tgccatagcg gcttattcct atatggcctt agttcctttg attcaaccgc cgattatgcg
     1561 tgccctgacc acccaaaagg aaagagaggt ggtgatggag cagttacgac ccgtctctaa
     1621 aacggaaaag atcatctttc ccattgttgt ggtaattatc accggcttgc tgttaccctc
     1681 cagtattcct ctgttgggga tgctgatgtt tggtaatcta ctccgtgaat ccggcctcac
     1741 tgaccggctt agtcaaactg cccaaaatga tttaaataat attattgtta ttctcttggg
     1801 cacagcagta ggtgcttcgg ccaaggcaga aactttcctt tcctcacaaa ccttgttgat
     1861 ccttgtacta ggcttggtcg cctttgctgt ggggacagcg gcgggcgtcc tgatgggtaa
     1921 actactgtat aaactgaccg gaggcaaggt taacccttta attgggtcgg caggagtctc
     1981 agcggtgccc atggctgctc gtgtttcaca gtctgaagga cgcaaagcca accctaataa
     2041 ttatttatta atgcatgcca tggggcctaa tgtagcggga gttattggtt ctgccatagc
     2101 ggccgggatc ttactgctgc ttttaggtta agagcgccgc tcacgaagtc agcgccagat
     2161 gagacaataa agaacctcac agtaagcgtt tgttggtcta aaatgtaaag ataatcttta
     2221 cattagactt tcggcgactg tgaggttcat ttacatgttg ccctgctgcg ctgtaaatat
     2281 acatcttata tctatagaat aaacataatt cttgtcctat ctcaatacaa taaaaagaaa
     2341 agcagtacaa ggcagggatg agaagatgtc gctagtaaaa aaacgtgtgc cacgctggac
     2401 aatcattatt attagccttt taagcttatt gtttagctgt tctttcattg attatcttta
     2461 tcaccgtgag cgcatttatg ctggtgtgtt tgttgcgcaa caggatttgg gaggtaagac
     2521 aacagaggaa gcggggcagc tattggcaga aataatagat gttggtgagc aaagtgtgga
     2581 attcatctac gacgagcttg cacaaacaaa aaaatttaat gagttgggca ttgcactaga
     2641 tctagatagt accattaatg tagctttgcg taccggaagg cagaacttgc atattttccg
     2701 ctatccagaa cgaattcgct tggccagcaa atctctacag ctggtaccgc aattcaaaat
     2761 tgatcaaact tcatttgaaa caagtctgca ggaaattttt actgctattc aactggaacc
     2821 agttgatgca cgtttgaaac tgtctgcgga ccgcaagagt gttaacattg aacctgatga
     2881 gccaggccgt atgctcgatg ttttagctgc ctatcgggct ttagaaacag ctttgcaatc
     2941 cacttacatt gatctgcagc ttaatttgcc tattgagccg gtagaggcgg agattacaag
     3001 tgccagccta gaggcattac aagtgactga gccagtagct tctttctcta cagcaatttc
     3061 cggtaatgcc aatcggctgc ataatattcg tcaagcagcc gcagccattg atcaaacgct
     3121 aatcccgcca ggtgcagtat tttcctttaa cgaaatagtc ggtcaaacaa ctgcggccac
     3181 aggttaccgg gcggcaccaa tcattatgaa tggtaagatt gttgatggta tcggcggcgg
     3241 catctgtcaa gtttcctcga ctttatataa tgcagtgctg ttgtcggatt tgcagcttgt
     3301 agaacgacgc aatcacggac tgcgtgtaaa atatttgccg ccggggttag atgctactgt
     3361 tgcttacggc agcatagacc tgaaatttaa gaacacccgc gctcatgcgt tatggctgcg
     3421 tatctttctt gatggtaata agttaaccac cactatctat ggtacgcaga ttcccggtca
     3481 ggaagtaaaa gtctacacta ccgatgtaca ggttatccct gctggtgaaa aaataattaa
     3541 aacggcagaa ttacccaaag gtaaacgaga gttagtaaca gtagggcagc ctggttatcg
     3601 agcgacagtt tggcgcagtg tttatttaaa tggtcaggaa gagaaaacag aaaaaatatc
     3661 tcaagatagc tataaggcta tgcctaatga atatcgtgta ggtacaaaag aactgcctgt
     3721 ttctggtgaa ccgtcagagc aaagtgagac agagaacgag taagtaaaaa gcttggcttt
     3781 cgccaagctt tttggtatac tggatgtaat aaaatgcgaa catagattcg gggtgggttt
     3841 gatgatgcgc gccattattc atgtggatat gaatgctttt ttcgcctcct gccaccaagc
     3901 tgaaaatcct gcttggcgcg gcaagccgct gcttgttgcc ggtgacccca aaaaacgtca
     3961 tggtattatc ttaactgctt cctatgaggc aaggcaatgt ggtatcaaaa cagctatgac
     4021 cgtctggcag gcaaaaaagt tatgcccaca gggcattttt attccccctg atcaccattt
     4081 atatttagct tactcggaaa agattttgac tatttttaga agttatacgc ctctggtaga
     4141 accattctcc atcgatgagg cttggttaga cgtcactggt tccgaacgtt tgtttggttc
     4201 accagaagag attggtcagc agctgcagca gcgaattctt gctgaattgg aaatctcctg
     4261 ttccgtgggc atagcgccca ataaatttct ggctaaaatg gcctctgaaa ggcaaaaacc
     4321 acgtggcttt acgatcattg gcccccaaga tattcgtacc gtactctggc cgctgccggt
     4381 cgagaagatg gtaggcgtgg gcagaaaaat agctcctgca cttaaggaaa tgggtgtaga
     4441 aactattgga caattagcaa agatgcctga gcggttattg gtaagccgct ttggtgttat
     4501 gggagaaata ttgcgggggc tggccaatgg agatgatgac tcccccgtcg atccacaagc
     4561 cctagatacg gctaagtcga taggtcattc cattactttg ccacgtgata ttaacgatcc
     4621 cgaggatgtc gctcgcattc tgttagaatt aagcgagcgg gtcggccgtc gcctacgcca
     4681 aggcggctat agcggtcgta ccattaccct gaccgtgaaa gatcaaaact ttgttaccat
     4741 tacccgggca tctacgttac agacacctgc ctatctcacg gaaactatct accaaacggc
     4801 acaagaaata tatcacagtc gttttgagcc atggcgtaaa gtgcgtttat taggcattgc
     4861 tatttctaat cttcaggcac gaggggaggg gctgcagctg acattatggg atcagactgg
     4921 tgaacgctta acaagcttaa ctgaaacagc tgataaaata aaagatcgtt ttggtgatta
     4981 tgctttacaa cgggccagtc tttataaaaa taaggattag tttagatagc acgatgacct
     5041 aacccaatgg ctacttcgtt taaatgaagc aggccaatgg caaaaaagat agcgactgct
     5101 acatggtcac ccttgcgcgc aatgcccact ttaccgccca cattaaggcc catcgctttg
     5161 ggcataattt gagcaatagc ttctcgtgcg gcaccagcga cgccgccttc ttcatggtgt
     5221 gtactttcga tgactccttc acgtttagca gcaacaacag ctcgctctaa aactttttgt
     5281 attgctgtta ggtattcacc gccgtaatct actgcggtgg aacgaattcc ttcggcgtaa
     5341 agctcctttt ttaactcttt ttccgcctca cgatctgccg ataaagctaa ccgaatagcc
     5401 gcactggcaa ttaatttact gtcaagttga ttcatggtca cacctctttt cccaacgcat
     5461 cttcttccaa ttataatata aaaaggcttg tgctgccagt ttgagataaa aagccagggg
     5521 agaggaaaaa acaagcgaac agatctgttc gcttgtttta tagattttac agtatttgtt
     5581 tcagttgtga gcagctaata tttttagaat cgcagtaaag tactaataac taaattacgt
     5641 acaatgccta atacaagata agcaagaatg ggtgataaat caataggtaa agcagtacgt
     5701 ggaattaaac gccggaatgg tgcaagcagt ggttctgtac cctcatatag taagcgaatc
     5761 aggggctgct ggggattatg tggtaaccac gataaaatta cacgcacaaa caacatgcca
     5821 tttaagatct taaaaaacca atctgcaaca taaactgcct gcagcacatt accaactcct
     5881 tgtttattat cttatttatt ctaccataag aagttagttt tgtgtaggga gggcttgtca
     5941 taaaatctca agatcactaa tttcgttcat ccacccaaat acgctctgct tgtgtagttt
     6001 tccccagttg ggtaaataga taaacctcct cggcgttagc tgcctctgaa gaaagcagct
     6061 gagcaattgc aggccagaca gcatcctcaa tccgggcagc taaaccacag gaagaggaaa
     6121 taacacgtgg tacagggatc attctgactg ccagctcttg cttgagcacc gttttttcaa
     6181 agcgtagcgc ctgtgatgtt gaataaaagg ttgctacata gtaattatcc acagtacacc
     6241 tctattttgt gatagtgata gtaaattcgc catgttcttc ttccgtcgta atttggtaag
     6301 ctaaggaaga aacaagtcgc cggatatttt cagcagcaac aaaactatca gctacgacca
     6361 caacagtacc atcgtgttct gcaagtgcac gtttggtatt gatgacaggt tcagggcagg
     6421 aaagaccacg gacatcaagt ttatagacca agagtgttac ctcctttttt acttactggc
     6481 agcggtaaag aaaacgcaaa gccgataatc accagaaaag atgctgccac cagtacacct
     6541 attttcccag ctactggaac accagtgccg ctggctgcta aaccaaaatt gtgcgctaca
     6601 gccgcaccag ccagcagtcc gagaaagacc attgcggcat ctgtatcccc ttctccggct
     6661 aggataacct gccgaagtgg gcagccgcca agcagcgtag ctcctagtcc taccacagct
     6721 aaacctatga agttccaaag ggcttcagta tgggcaacag gttgctctag aaaacctagt
     6781 ttgaaatagc caaaagcaat attcagcact aaagcggcta caaagatagc aatagtacca
     6841 tacagtaagt gcgggtcgcg gatcaacatc agatcacgaa aacaaccggc ctgacaaagc
     6901 ctggtacgct gcactaaaac accgaccagt aaacccgcgc ccaaagcaag caaaatggga
     6961 gcatgggctg cgcctggtcc actttcacta aagtaaataa aacctggtgc taaaactagt
     7021 aatattaata agatcaaagc gaaggccggt atgacaagac cattagttgt cggcagcttc
     7081 tgactgcgcc ctaaagagaa accttttttc aaaaattgca caccaataaa aatgccaaaa
     7141 ataaaaccac ccaagccaac taaagcattt aagtcaccag cggccagtcg taaaaccatc
     7201 cgcaagggac atcctaaaaa gaccagagcg ccaatcatca tggcaacacc cataaaaaat
     7261 cgaatcagcg gcgaagaacc gccgcgagca cgaaactccc catttttcaa agcaataaga
     7321 aacgctccca gaataaaacc aataatttca ggacgtatgt actgcacagg agccgctcga
     7381 tgtaaaccaa gcccaccggc tatatcacga ataaagcagg cgacgcaaat tcccatgttt
     7441 accgggttac caaagtaagc taggagcacc cctaaaccgc ccaatacacc accggctaca
     7501 attatttttc ttctctccaa ataattacct cccttaaata
//
LOCUS       DASRMS010000121        23010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_232358, whole genome shotgun sequence.
ACCESSION   DASRMS010000121 DASRMS010000000
VERSION     DASRMS010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 23010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23010
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232358"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            641..838
                     /locus_tag="VFC74_05615"
     CDS             641..838
                     /locus_tag="VFC74_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016412724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK24845.1"
                     /translation="MTTERKFTIEPSRFIHAEEVAEILGVSVSSAYRIIKRLNNSLKD
                     QDKITVAGKISRRYFEEKVML"
     gene            911..1990
                     /locus_tag="VFC74_05620"
     CDS             911..1990
                     /locus_tag="VFC74_05620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZK24846.1"
                     /translation="MPTYKNEATGSWYCAFYYTDWLGNRKKKKKEGFKTQREAKTFER
                     QFIEKAEASPDMSFGTLVSIYMEDCKTRLKPTTYSGKECLINTKLLPFFEEMPINKIE
                     PATIRKWQNTLMEDESDYSQTYLKTIHNQISAIFNFASKYYKLQTNPARVCGSMGKKN
                     AESMLFWTVEEFRKFLPFVSDKPVSKLAFEILFWTGIRSGELLALTLNDFDFDAKTLS
                     ITKNYARHEGEDLIMEPKTPKSRRLIAVPTFLCALVQKYAEQLYDYEPNERLFPVTKS
                     YLHSEMTRGTKKSGIKRIRVHDLRHSHASMLIEEGFSALLISERLGHESIETTLQTYS
                     HLYPNKQIEVANRLDQMNKPDSPIE"
     gene            complement(2083..3627)
                     /gene="guaA"
                     /locus_tag="VFC74_05625"
     CDS             complement(2083..3627)
                     /gene="guaA"
                     /locus_tag="VFC74_05625"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HZK24847.1"
                     /translation="MELVTKELAVILDFGGQYTQLIARRIRDLNVYCEILPYTAAVAQ
                     IKDLQPRALIFSGGAKSVYGPEAPAVDSEVYQLGLPILGICYGMQLMAKDLGGVVEAA
                     ARREYGQTLLTVQEDRDLFAGLEKEISVWMSHGDFVKQAPPGFTVTATTSNTPIAAMT
                     NSEQHFYAVQFHPEVNHTSWGSEMLKNFLFSVAGFTGDWTTGSFIDDTVQEIREAVGE
                     HQQVVCGLSGGVDSSVAAVLVHKAIGDRLTCVFVDHGLLRKNEAQQVMETFTRTLKMK
                     VIHVDAATEFLGKLAGVTDPEQKRKIIGAEFIRVFEREAAKIGKIDYLVQGTVYPDII
                     ESGTAAAAVIKSHHNVGGLPEDLKFKLIEPLSHLFKDEVRRVGEALNIPEEIVWRHPF
                     PGPGLAIRVLGEITKEKLEILRAADAIMVEEIKNAGLYREIWQAFCVLPNIRSVGVMG
                     DKRTYAYTIALRAVTSHDGMTADWYRFPHEVLARISNRIVNEVPHVNRMVYDITSKPP
                     STIEWE"
     gene            complement(3880..4689)
                     /locus_tag="VFC74_05630"
     CDS             complement(3880..4689)
                     /locus_tag="VFC74_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HZK24848.1"
                     /translation="MAEKKIKPEFAKWTNPPATKIKRVVAVISGKGGVGKSSVTALLA
                     VTLTRAGLKVGILDADITGPTIPQMFGVNEMPQGGEQGMTPPQSKLGIKIMSLQLLMD
                     DEDAPAIWRAPMLNGTIKRFWTDVMWGELDCLLVDLPPGTGDVPLTVMQSLPLAGVMM
                     VATPQKIAVGVVRKAIKMAERMGVPVLGLVENMSYVGCGSCGEKNYIFGPSAGGQVAE
                     EFGLPFLGAVPIDPLLVELADSGKIEEYKHPVFAAVMEPLTEKNLIFAGTN"
     gene            complement(4810..6432)
                     /gene="groL"
                     /locus_tag="VFC74_05635"
     CDS             complement(4810..6432)
                     /gene="groL"
                     /locus_tag="VFC74_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119663.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HZK24849.1"
                     /translation="MAKDLLFREDARRALERGVNKLADTVKVTLGPKGRNVVLDKKFG
                     APLITNDGVTIAKDIELEEPFENLGAQLVKEVAVKTQDVAGDGTTTATLLAQAIIREG
                     IKNVTAGANPMSIKRGIEKATNAIVAEIKAQSRPIETKEAISQVAAISADDHNIGELI
                     AEAMEKVGKDGVITTEESRGFNTELKVVEGMMFDRGYISHYMVTDTEKMEAVLDEPYI
                     LATNRKISNVQDILPILEKVVQSGKPLLLVAEDVEGEAVATLILNKLRGTFNCVAVKA
                     PGFGDRRKAMLEDMTILTGGQVISDELGIDIKSVDLDMLGRARQVRVTKEETVIVDGA
                     GEASAIQGRIVQIRNQIEDTTSEYDKEKLQERLAKLAGGVAVIEVGAATEVEMKEKKL
                     RIEDALSATRAAVEEGIVPGGGTSYIDALPALDNIAAEGDELTGVNIIRRALEEPVRQ
                     IALNAGMEGSIVVEKVKAAAPGVGYNAAANTYVDMIDAGIPDPAKVTRYALQHAASIS
                     AMFLTTEAVVVDQPEEDKGGPAGGGMPQMPMM"
     gene            complement(6480..6767)
                     /gene="groES"
                     /locus_tag="VFC74_05640"
     CDS             complement(6480..6767)
                     /gene="groES"
                     /locus_tag="VFC74_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HZK24850.1"
                     /translation="MKIKPLGDRVVIKVLEAEEKTASGIVLPDKAKEKPQEGEVAAAG
                     NGRILDNGTRVEMDVAVGDRVLFSKFAGTEVKLDGVEYLIMRQDDILGKLV"
     gene            7233..8915
                     /locus_tag="VFC74_05645"
     CDS             7233..8915
                     /locus_tag="VFC74_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HZK24851.1"
                     /translation="MPKYRHFKRYREIANNVVRHGFGHLLTQLGMAQFLPSVRRAQES
                     DADILRYSRAQRLRMLLEELGPTFVKLGQLLSTRSDLIPRDFLVELASLQDRVPSFPY
                     ENVTQIIAQELGQSIEEVFADFKEEPLAAASIGQVHRAQLHSGEYVVVKVRRPQIIEQ
                     MQMDLEILIDIATIADKRTAWGQMYHLADIALELQSSIVEELDYLNEAENADQIRRNF
                     RHRDDVIIPKIYWQQTTSAVLTMEYVQGVKLNTPDALRSAGHEPAKVVNRLVDLMYTQ
                     IFEHGWFHADPHPGNLAVAADGRLIFMDFGIVGRLKGERKSQFVLFLLGIVTKNPRQL
                     VRSLQNMGVLSHRVDRKALRRDVERLLDKYLDAALRKINLGKAVAEIFSLTYQYHIRI
                     PAEFTLLGKTIMTLEGVIEDLDADLKLVELLQPYAGRLARDRFSYESLKDTATEHFFE
                     TSDFLISLPRRLNDLLDRLDTEGLPFQINYPDIDKAFLHLDRLANRLTFSIVLLSFSI
                     IMAALIIGAGFITAIAGETALLWRLPIMEIGFVVATGMATWLIAAIYRSGKL"
     gene            9359..10756
                     /locus_tag="VFC74_05650"
     CDS             9359..10756
                     /locus_tag="VFC74_05650"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24852.1"
                     /translation="MKKPLSPALKTFYGIGDLGFSLMTSVGMYFQTYFLTDVAGFNLA
                     LVGLIITIPGIFDAVFAPIYGAIIDAVKPMKWGKYRSWLILTPPLVVVLMTMTYTKIG
                     TDAMAATIIIACTLLARPLFNIPWVANVALISVLANNPSERVLLSSRRAFWTSLSRVF
                     FSYIGPPLAAYFGGRTNPVAGYSILQFLMATMMMIGYFIQFKMTDGYEETTSKTAQSA
                     KQTAQKPKEKTSLKDLLSNLFQNPPLMALMFADFGRYLGMFTMTACIAYYFTYVAENM
                     DLMPPFMLITALAGVAGSLATPTLNKKLKTRGTSIVGLLLAGGLMLFSKIVGLQTYIF
                     MAICTLAQFFMGMLASTLVALYSDTVVYGEWKTGKNTAGFIMGLMNLPLKIGSLARGV
                     VMTAALAGIGFIAGMEPTLALKDGILNIYVVMPGIGLMLAGVMLLFGFRLTDDKLQEM
                     TEEIESRKGKVIVEQ"
     gene            complement(11005..12378)
                     /gene="argH"
                     /locus_tag="VFC74_05655"
     CDS             complement(11005..12378)
                     /gene="argH"
                     /locus_tag="VFC74_05655"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HZK24853.1"
                     /translation="MKLWGGRFNKETDALMEAFNASIGFDQRLYAEDIGGSTAHCRML
                     AACGIITQAEADLIINGLQDIQAEIERGEFEFTVALEDIHMHVEKRLIELIGPVGGKL
                     HTARSRNDQVALDLHLYVKKECLETVALIKNLQEVLLQTAAAYQDVIMPGYTHLQRAQ
                     PILFAHHLLAYFWKLQRDQERFLETYQRADWMPLGSGALAGTTFPINREMVAEDLGFG
                     QLYENSMDAVSDRDFVVEFLAHASLLMTHLSRLSEDLILWNSQEFGFVELDDAFTTGS
                     SIMPQKKNPDVAELVRGKTGRVYGHLLAMLTVLKGLPLTYNKDLQEDKEGLFDTVDTI
                     KMCLTLYAAMLRTLRVNQERMYAAVAGDFASATDLADYLVRRGLPFREAHAVVGRLIA
                     WCIAEKRPFSDLTATELQAHSPLFTDKALELLSPEACVAARTSRGGTAAVAVAQQLQK
                     AKAALAE"
     gene            complement(12387..13589)
                     /locus_tag="VFC74_05660"
     CDS             complement(12387..13589)
                     /locus_tag="VFC74_05660"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HZK24854.1"
                     /translation="MKKIVLAYSGGLDTSVAITWLKEKYNVEVVAMAADVGQGEELEP
                     LQEKAINSGASKLYIEDIREEFMRDFVFPYIKSGAIYEQKYLLGTAIARPVIAKRLVE
                     IAEKEGADAIAHGATGKGNDQVRFELTVKALAPHLKIVAPWREWEIKSRDDAMDYAAK
                     HGIPVPVTKAKPYSMDRNLWHLSYEGGILEDPDNEPDQEMFMLTTDPSQAPETPEYVE
                     IGFEQGIPLSVNGQILEPVELLTTLNTIAGRHGVGRVDMVENRLVGMKSRGIYETPGG
                     TLLCFAHRELEALTLDRETAHYKETAAIKYGELVYYGLWYSPLREALDAFFDSTQRTV
                     TGTVRIKLYKGNITVAGRTSPFSLYREDLATFGEDVVYNQQDAEGFINLFGLPLKVQG
                     LLAKSQKK"
     gene            complement(13586..14398)
                     /gene="murI"
                     /locus_tag="VFC74_05665"
     CDS             complement(13586..14398)
                     /gene="murI"
                     /locus_tag="VFC74_05665"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HZK24855.1"
                     /translation="MQKLAEKKEKPIAFFDSGVGGLSVLAVAVHCLPHEDFIYYGDTA
                     HAPYGRKSKAEVQALSLQVAEILMARGCKALVVACNTATSAAVHLLREQLPIPVIGME
                     PAVKPALEHGDQGKVLVMATPLTLQEEKFHDLCQRCEVDLAKVLLVPCPTLVELVEQG
                     CIAGRQVEQELVKIFAAIDTSSVATVVLGCTHYLFLKEALTRIFPLGVSFIDGNLGTV
                     RQLTRVLANLDLLREEEQVSSKVELLTSGGESARQLLQKLYTSAYEKMEGLK"
     gene            complement(14420..15352)
                     /gene="argF"
                     /locus_tag="VFC74_05670"
     CDS             complement(14420..15352)
                     /gene="argF"
                     /locus_tag="VFC74_05670"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HZK24856.1"
                     /translation="MSSLQGRDFLSVNDFTPDELLEILTFAQELKIKQRTGQAAALLP
                     GKTLGMIFQKASTRTRVSFEAGMFQLGGYALYLSAADMQIGRGEEIKDTARVLSRYLD
                     GIMIRTYAHRDVEELAAYAEIPVINGLTDLLHPCQAMADLLTILEQQGTLSGLKLAYL
                     GDGNNMAHSLLLACSKLGINVAVASPLGYQPSAAIVADAQANAQKHGSTVTVTTEPAA
                     AAADADVLYTDVWASMGQEAEQMSRAKIFKSYQLNNQLLAQAKSEAIVMHCLPAHRGE
                     EISEEVLEGPQAVVFDQAENRLHVQKAIMAKLLS"
     gene            complement(15349..18606)
                     /gene="carB"
                     /locus_tag="VFC74_05675"
     CDS             complement(15349..18606)
                     /gene="carB"
                     /locus_tag="VFC74_05675"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZK24857.1"
                     /translation="MPRDEALKRVLVIGSGPIIIGQAAEFDYAGTQACKALREEGCQV
                     ILVNSNPATIMTDPDLAERVYLEPLSLEFVSQVIARERPDGLLPTLGGQTALNLAKEL
                     AEAGVLAKYHVRLLGTSLETIKHAEDRELFKETMQEIGEPTAPSIISSSLQEAFDFAQ
                     AVGYPLIVRPAYTMGGSGSGFATDDQSLRDIVSRGLKHSLIGQVLLETSVAGWKEIEF
                     EVMRDAQDNCITICSMENFDPVGVHTGDSIVFAPALTLEDTEEHMLRNAALKIIRALK
                     IEGGCNIQFALHPTDKKYIVIEVNPRVSRSSALASKATGYPIAKVATKIAVGLQLEEI
                     LSSGSGKAAACFEPTIDYVVCKIPRWPFDKFQAANRQLGTQMKATGEVMAIGQTLEEA
                     LLKAVRSLEIGVTGLQMAELKTRSDRQLAAELAAPTDERLFVLAEMLRRGGSVAEAAE
                     ISSIAPYFIDKIKKIVAAELALGKLNLTALTAKQLRHYKELGFADAEVAKILGVSSEA
                     VRAQRKSLALAPAYKTVDTCAAEFDTLMPYYYSTYQAEDEVNCSKQEKVLVLGSGPIR
                     IGQGIEFDYCSVHSVWALKESGREAIILNNNPETVSTDFDCADKLYFDPLTVEDVLAV
                     VEKEKPLGVVVQFGGQTAINLAGPLQQAGVKLLGTPLETIDLAEDRQKFNQLLRLLGI
                     PQPEGVAVSSPHEALGAAHKIGFPLLVRPSYVIGGRAMQIVYNEEQLYTYLQLAANVT
                     PEHPVLLDRYLLGTEVEVDAVCDGTTVLIPGIFEHIERAGVHSGDSMAVFPPQSLTPE
                     LMETLTEYTVRIALALGIRGLVNIQYVVWRERVYVLEVNPRASRTVPIISKVTGMPLV
                     ALATRIMLGETLQEMGYGHGLVPAPPYVTVKAPVFSFAKLQQLDISLGPEMKSTGEVL
                     GMADDFATALYKSMLASGFSFPQRGNILASVADKDKQELIPLLQQAHAGGFKIYATAE
                     TAQALQKAGAANVETVNKVGEGLPNVLDLIREGKIDLAVNTPTYGKKITSTGYQVRRA
                     CVEYQIPCLTSTDTARAFFEVLTRKTAGQLQPTVVALQDYLAQRGQRHGRKDVAE"
     gene            complement(18622..19809)
                     /locus_tag="VFC74_05680"
     CDS             complement(18622..19809)
                     /locus_tag="VFC74_05680"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HZK24858.1"
                     /translation="MELKALEKKTIINTYNRQPEATLLLQRGEGAYVWDDAGKRYLDF
                     VSGLAVNNLGHCHPQIVEAIYKQAQQLMHTSNLYYTEPQIRLAEQLVQHSCADKVFFC
                     NSGAEANEAAFKLARKYGKLKKGEAAFEIITAKRSFHGRTLATVTATGQPKYHKGFEP
                     LVAGFRYAEFNNLQSFKDAVTAQTCAILIEPVQGEGGVYPATLEFLQGLRQLCDKHQL
                     LLIFDEVQCGMGRTGKLFAYQHYGVEPDVFTLAKALGGGLPIGVMAAKGEAATTLVPG
                     EHASTFGGNPVVCAAANVAVSILAEADFLNRVAELGAYFQQKLQALAYPHLVEVRGLG
                     LMIGLEIAGDAARVVPLCQEKGLLVNCIGGKTLRFLPPLIVTEKEIDAAVTILEQTLT
                     AID"
     gene            complement(19820..20713)
                     /gene="argB"
                     /locus_tag="VFC74_05685"
     CDS             complement(19820..20713)
                     /gene="argB"
                     /locus_tag="VFC74_05685"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HZK24859.1"
                     /translation="MNNDVQQLIAKADVLIESLPYLRSFADKTFVIKYGGHAMVSEEL
                     KKSVMMDIILLKYIGINPIVVHGGGAEVTKMLQRLGQESQFVNGLRVTDKEAIEVVEM
                     VLTGKVNKQIVALINQYGGKAVGLSGKDANLLVARRRAPEQLSGSEEVVDLGYVGEIT
                     KVNPEVIHTLSKEGYIPVISSIAVGLEGESLNINADHVAGELAAALAADKLILLTDVE
                     GIFVEAGVPSSLISALPKAKALAMIAEGKINKGMIPKVEACIMALEHEVNRTHIIDGR
                     VPHSLLLEIFTDHGIGTMVTS"
     gene            complement(20761..21969)
                     /gene="argJ"
                     /locus_tag="VFC74_05690"
     CDS             complement(20761..21969)
                     /gene="argJ"
                     /locus_tag="VFC74_05690"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HZK24860.1"
                     /translation="MKFRKVEGGVTAARGFKAAGIAAGIKKKDKKDIALIYSQVPAVA
                     AGMFTKNRFQGAPVLVSREHLADNKAQAIIVNSGNANACTGEQGLKDAQRMAALTGEL
                     LGLQTQDIVVASTGVIGQYLPMARLETGIHDAVEQLQAEGGPLAAEAIMTTDLTAKIT
                     AFQLDIGGEIVTVGGIAKGSGMIHPNMATMLAFITTDCAIAPALLQKALQYSVERSYN
                     LITVDGDTSTNDTVVVLANGAAANLVISEENEDYHRFLMLLQRVNTELAQSIVRDGEG
                     ATKFLAVTVKHAATYEDAKTLARGILNSNLVKTAFFGEDANWGRIISAMGQTEVEFFP
                     ERVDVFLGDLQVTQFGQGLLFDEAQAKAILAQQDIRITVDLQMGEEKVTAWGADLSYD
                     YIKINASYRS"
     gene            complement(21982..>23010)
                     /gene="argC"
                     /locus_tag="VFC74_05695"
     CDS             complement(21982..>23010)
                     /gene="argC"
                     /locus_tag="VFC74_05695"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HZK24861.1"
                     /translation="VFIMLKVAVLGATGYAGKELVSILAHHPYARPYYITSESFSGQS
                     FAQIYPEFRAVCELPLEKLELTKVAACDFIFAALPHGLSVKEVPKLLNTGIKVVDFSG
                     DFRLKDASLYPTWYGYEHSSQENLALSVYGLPEYNRAMIRDAELIANPGCYPTSVLLA
                     LKPLLAEGLIEAEDIIVDAKSGVSGAGRSPKQAFHFPECTENFKAYKIAAHQHTPEIE
                     QELSNLAGKPVTLIFTPHLVPMIRGILSTIYVRSAAGVDEATVSAAYQKHYAQEPFIR
                     LLPAQELPETKYVSGTNFCDLAFRLDPRTGRLIVVSAIDNLVKGAAGQAVQNMNIMQN
                     WPEEAGLK"
BASE COUNT         6149 a         5848 c         4792 g         6221 t
ORIGIN      
        1 aaatctcatg gcttatgaga gggactttta tcttctcata aaatcgatct ttgacaagtt
       61 catataggag aaccggatac ttttggtgca cgagccggaa ggacaagcca ggcagcaggt
      121 acgccatgac cttcgtttaa gaagagagcg agacataccc atgctgcagg tcgatcttgg
      181 cagatcggct ggccatgacc ttgtgcatca gataatgata cttccgtata gtcacagtcc
      241 gagcgtgatc aaaccgtcgc aggcaatgag tacggcccgc catcagaatg gagggaggtg
      301 aaagtcctat gaaagcgtaa gccagcgcat gtccggtccg tccaaagaac cagaatcaaa
      361 tatagcagcc gcactgcatt caagcattct taatgatgct tcatcctacg cggtgcggct
      421 gctttgattt tcatgtcaat tttattgatg gttatattcc actgcatcga caacattgca
      481 tgaaaccgaa acgtgatacc ctcaccttaa gttcatcatc cctgcagaat taaataaaac
      541 ccctgcattc atcctggaca taccaaaccg ccggggcgct tttaccgacg gcttggtata
      601 ttcaggagat ccatcctgag aaagaaagga gaaaacgaaa atgacaaccg aaagaaaatt
      661 caccatcgaa ccatcaagat ttattcatgc tgaagaagtc gcagagattt taggtgtgtc
      721 ggtatcctct gcgtaccgga tcatcaagcg tcttaacaat tctcttaagg accaggataa
      781 aatcaccgtg gccggtaaga tcagcagacg ctatttcgaa gagaaagtga tgctgtaaat
      841 actaaacacg taaagccaca tgacacgaga tttcatggtt cagtgctttg cggccagaga
      901 gggtgatgct atgccaacct ataaaaatga agccaccggc agctggtatt gtgcctttta
      961 ctataccgac tggttaggca accgaaaaaa gaaaaagaag gaaggcttta aaacccaaag
     1021 agaagccaag actttcgaac gacaattcat tgagaaggcg gaagccagtc ccgacatgtc
     1081 ctttggcacc ctggtgtcca tctacatgga ggattgtaag acacggctca agccaaccac
     1141 ctacagtggc aaggaatgcc tcatcaacac aaagctgctg ccttttttcg aagagatgcc
     1201 catcaacaaa attgaacccg caacgattcg caaatggcaa aataccttga tggaggatga
     1261 gagcgactat tcacaaactt atttgaaaac catccacaac cagatctccg ccatttttaa
     1321 ctttgcctcc aagtattata agcttcaaac caaccctgcc agagtgtgcg gcagcatggg
     1381 taagaaaaat gcagagtcca tgttgttctg gactgtagag gaattcagaa aatttctccc
     1441 ctttgtctcg gataagccag tttcaaagct ggcctttgaa atcctgtttt ggacgggtat
     1501 ccgatcgggt gaattgttgg cattgacctt gaacgatttt gactttgatg ccaaaacatt
     1561 gtctatcaca aaaaactacg ctcgccatga gggcgaagat ctgatcatgg aacccaagac
     1621 acccaagagt cgccgcctga tcgcagtgcc aacctttctc tgtgctctgg tccagaaata
     1681 cgccgagcag ctttatgact acgaaccaaa cgaaagactt ttcccggtaa ccaaatccta
     1741 tttgcacagt gagatgacac gagggacgaa gaaatctggc atcaaacgca ttcgcgtaca
     1801 tgatctgaga cattcccatg cctccatgct gattgaggaa ggtttttcag ccctcctgat
     1861 ctcagaaaga ctgggacacg aaagcatcga aacgacgctt cagacctatt cccaccttta
     1921 tcccaataag cagatcgagg tcgccaatcg cttggatcaa atgaataaac cagatagtcc
     1981 aattgaataa ttacaaaagg acctgtagta cgcttttagt acggtcacca aaaagcaaac
     2041 cctgaaacca cgttaaatca atggtctcag ggtttttgac tgctattccc actcaatggt
     2101 cgaaggcggt ttggaagtaa tatcatagac catacgatta acatggggga cttcgttgac
     2161 aatgcggtta gaaatacgtg ccagcacttc gtgggggaaa cggtaccagt cagctgtcat
     2221 gccgtcatga ctggttacag cacgcagggc aatggtgtag gcataggtgc gcttatcgcc
     2281 catcacacca accgagcgta tattcggcaa gacacaaaaa gcctgccaga tctcccgata
     2341 taagcctgca ttttttattt cctcaaccat aatcgcatct gctgcccgta agatttctag
     2401 tttttcttta gttatttcac ctaacacccg aatggctaaa ccgggaccgg ggaagggatg
     2461 ccgccagaca atctcctcag gaatatttaa ggcttcacca acacgacgca cctcatcctt
     2521 aaacaagtgg cttaaaggtt ctattaactt aaacttcaga tcttccggca gaccaccgac
     2581 attgtgatgg gatttaatca cagccgcagc ggcagtgccg ctttcaataa tatctggata
     2641 aacggtgccc tgtactaaat aatctatttt gccaattttg gcggcttctc gttcaaagac
     2701 acgaataaat tccgccccaa taattttacg cttttgttcc ggatcggtaa cccctgctag
     2761 tttgcctaga aattcagtcg cggcatctac atgaattact ttcattttta acgtcctagt
     2821 gaaggtctcc atcacttgct gtgcttcgtt tttccgcagg agcccatgat caacaaagac
     2881 acatgttaag cgatcaccaa tagctttatg taccagcaca gcggccacgg aactgtccac
     2941 accgccggaa aggccgcaga ctacctgctg atgctccccg acagcttccc ggatttcctg
     3001 cacagtatca tcgataaaag aacctgttgt ccaatctcct gtaaaacctg ctacgctaaa
     3061 caaaaagttc tttaacatct cgctgcccca cgaagtatga ttgacttcag gatgaaactg
     3121 gaccgcgtaa aaatgttgtt cagaattggt catggctgcg atcggtgtat tggaagttgt
     3181 tgcagttacc gtaaaacccg gtggcgcttg tttcacaaaa tcgccatgac tcatccaaac
     3241 agagatttct ttctccaggc cagcaaaaag atctctgtcc tcctggacag ttaaaagtgt
     3301 ttgcccgtac tcacgtcgtg cagcagcctc taccaccccg cctaaatcct tggccatgag
     3361 ctgcatgccg tagcaaatac ctaaaatcgg taaacccaat tgataaacct ctgaatccac
     3421 ggcgggtgct tctggaccat aaacgctctt agctccgcca gaaaaaatta gggcacgtgg
     3481 ttgtaagtct ttaatttgtg ccacagctgc tgtgtaaggc agtatttcac aataaacatt
     3541 aaggtcccta attctgcgag caataagttg ggtatattgt ccaccaaaat ctaaaatgac
     3601 tgctaattcc tttgtcacta actccacctc actacttata aattacagct gacttgctta
     3661 acagcgctga ctacgttaaa catactgcta tgctagtctc ccctcccacg ggccagaata
     3721 ctttacagct gcagcaagcc cttggcttcg acaaaattgc cagcatgaac attatacatg
     3781 gagtgagggt tcttggacaa ctatttgtca agtttttctt agtggcgttt acttgaaatg
     3841 ataattgcta ccgggttaaa tggtgaaggg ctgctgcaat taattcgtac cggcgaaaat
     3901 aagatttttt tctgttagcg gctccattac tgctgcaaaa acaggatgtt tatactcctc
     3961 gatcttgccg ctatcggcaa gttccaccag cagtgggtcg ataggcacgg cacctaaaaa
     4021 gggcaagcca aattcttctg cgacctgccc accggcactg gggccaaaaa tatagttctt
     4081 ttccccacaa ctgccgcatc ccacataact catattctcc actaaaccaa gcactggcac
     4141 ccccatcctc tcagccatct taatggcttt tctgacaaca cccacagcaa tcttttgtgg
     4201 tgttgccacc atcatcacac ccgccaaagg tagggactgc atcactgtca gcggtacatc
     4261 gcctgttccc ggtggtagat ctaccagcaa acaatctaat tctccccaca tgacatccgt
     4321 ccaaaatctt ttaatggtac cattcagcat cggggctcgc cagatggccg gtgcatcttc
     4381 atcatccatc aacaattgta aagacataat tttaatgccg agcttacttt gtggcggtgt
     4441 catcccctgc tcgccgcctt gcggcatttc attgacgcca aacatttggg gaatagttgg
     4501 accagtaata tcggcatcaa ggatgcccac ttttaagcca gctcgtgtta acgttacagc
     4561 aagcagtgca gttacagaag atttgcccac gccacccttg ccactgatga cagctactac
     4621 tcgtttgatc ttagtcgctg gaggattagt ccatttggcg aattcaggtt ttattttttt
     4681 ttcagccatt aagttttccg ctcctttact taaactcact agtagcatca aacaaaaggc
     4741 cgcttttaat gcaagcttta agcgaaagaa aaagtacccg cctgtgcggg tacttttagt
     4801 agttagtttt tacatcatgg gcatttgggg cataccgccg ccagcggggc cgcctttgtc
     4861 ttcctctggc tgatcaacaa caactgcttc ggtggtcaag aacatcgcgg aaatgctagc
     4921 tgcatgctgc agagcatagc gcgttacttt agccggatca ggaataccag catcaatcat
     4981 gtccacatag gtatttgcag cagcattata gccaacacca ggtgcggcag ctttaacttt
     5041 ttctaccaca atggaacctt ccatgccggc gttaagagcg atctgccgca caggttcttc
     5101 cagcgcacga cgaataatat ttacaccggt cagctcatca ccctcagctg cgatgttatc
     5161 aagagcagga agtgcgtcga tgtaagaagt gccgccgccg ggaacaatac cttcttctac
     5221 tgcagcacgc gttgcagaaa gagcatcctc aatgcgtaat ttcttttctt tcatttcgac
     5281 ttctgttgca gcaccaactt cgatcactgc aacaccgccg gccaatttgg ctaaacgttc
     5341 ttgaagtttt tctttatcat actcagaggt agtatcttca atttggttgc ggatttgtac
     5401 aattcgccct tgaattgcag atgcttcccc ggcaccgtca acgataactg tttcttcttt
     5461 agtgacgcga acttgacgag cgcgaccaag catgtcgaga tccacactct taatatcgat
     5521 accaagttca tcagaaataa cttgtccacc ggttaaaata gtcatgtctt ccaacattgc
     5581 tttacgacga tcaccaaaac cgggagcttt gacagccaca cagttaaagg tgccacgtaa
     5641 tttattgaga attaaagtag cgactgcttc accttctaca tcttcagcca cgagcaataa
     5701 gggtttacct gactgtacca ctttttccaa aatcggcagg atatcttgta cattgctgat
     5761 tttgcggtta gtagctaaaa tgtaaggctc gtccaggaca gcttccattt tctctgtatc
     5821 tgtgaccata tagtgagaaa tataaccgcg gtcaaacatc ataccttcaa caactttaag
     5881 ttcagtgttg aagcctctgg attcttctgt ggtaatgacg ccatctttac ctaccttttc
     5941 catcgcctcg gcaatcagct caccaatgtt atggtcatca gcagaaattg ctgctacctg
     6001 agagatagct tcttttgttt cgatgggacg ggattgggct ttaatttcag ccacaatggc
     6061 gttggttgct ttttcaatac cacgtttaat ggacatgggg ttagcacccg ctgtgacatt
     6121 cttaatacct tcgcgaataa tagcttgtgc caataaagtt gcagtcgttg tgccatcgcc
     6181 ggctacatct tgtgttttaa cagcaacttc tttcaccagt tgagccccta aattttcaaa
     6241 gggttcctct aattcaatat ctttagcaat ggtaacaccg tcgttagtaa tgaggggtgc
     6301 accaaatttc ttgtcaagca ccacattgcg gcctttcggg ccgagggtaa ctttgacagt
     6361 atcagccagt ttattaacgc ctctttctag agcacggcgt gcatcttcac gaaataatag
     6421 atctttggcc attgtttgtc tctgcctcct ttattttcag ttgatatgtg cgtgttaagt
     6481 tacacaagtt tgcccaagat atcatcttgg cgcataatta agtattcgac accgtctaat
     6541 tttacttcag tgccagcgaa tttagagaac agtacgcggt cgccgacggc aacatccatc
     6601 tctacacggg taccattatc caagatgcga ccattgccag cagcggcaac ttcaccttcc
     6661 tggggttttt ctttggcttt gtcaggcagg acaatgccac tagccgtttt ctcttcggcc
     6721 tcaagtactt tgatgaccac acgatcacct aggggtttga ttttcatttg aagacctcct
     6781 ttattaagtc ggtttattgt tgttagcact ctctggttgt gagtgctaat tatcacactt
     6841 gttatggtat aaaaaaagtg gtctgaaagc aaatgccccg agggcgtcat ttaggcaaat
     6901 atacgcattt atacacaatt agcacaatat atcagccttt ttctttattt atctatattt
     6961 tcctttattc acttttttag aggctgtaaa ttcttcacca agtgtttaaa ttcataccaa
     7021 agcagctgct atagacactg gtactagcta gagggggagc ctcgaccagg aacggacagc
     7081 tacagctctt aacttcaata gtggatagta tacacgttgc tcttcttttt atgcattttg
     7141 cagtcctaga tctgagccgc cttatgctgc tgacgacagc ttgacatcac caatccttac
     7201 cagcataatg gaagtacgga ggtggccaag tcatgcccaa ataccgtcat tttaagcgtt
     7261 acagagaaat tgccaataac gtagtccgtc acggctttgg tcatttgttg actcaacttg
     7321 gcatggcaca attcctcccg tccgtgcgcc gggcacaaga atctgatgcg gatatcttac
     7381 gctactcccg tgcccagcgc ttacgcatgc tgctggaaga actaggccct acctttgtaa
     7441 aacttgggca attactatca acacggtcag acctaatacc acgtgatttt ttagttgaat
     7501 tagcgagcct acaggatcgc gtcccatctt ttccctacga aaatgtcact cagatcatag
     7561 ctcaagaatt aggtcaaagc atcgaggaag tatttgctga tttcaaagaa gaacccctgg
     7621 cagcagcctc cattggccaa gtgcaccgtg ctcaactaca tagcggggag tacgtggtag
     7681 taaaagttcg tcgaccacag atcattgaac aaatgcagat ggatttagaa atattaatcg
     7741 acatcgccac cattgctgac aaaagaacgg cttggggcca aatgtaccat ttagcggata
     7801 tcgctttaga gttgcaaagt tctattgtcg aagaattaga ctatttaaac gaagcggaaa
     7861 acgctgacca gatccgccgt aactttaggc atagagatga cgtaattatt cccaagatct
     7921 actggcagca aactacttca gctgtcctca ccatggaata tgtacagggt gtcaagctca
     7981 acactccgga tgcactgcgc agcgcaggac atgaacctgc caaagtagtt aaccgcctag
     8041 ttgatttaat gtatacacaa atatttgagc atggttggtt tcatgctgat ccccaccctg
     8101 gcaatctagc agtggcagca gatgggcggc ttatttttat ggattttggc attgttggcc
     8161 gcctcaaagg cgaaagaaaa agccagtttg tcctcttttt attaggaatc gtcaccaaaa
     8221 atcctcggca gcttgtgcgc tccttacaga acatgggagt actttcacac cgtgtagacc
     8281 gcaaagccct gcgacgtgat gtggagcgct tgctggataa gtacctggat gccgcactgc
     8341 ggaaaattaa tctcggtaag gctgtagcag aaattttttc tcttacctat caataccata
     8401 ttcgcattcc cgcagagttt accttgctag gaaaaacgat catgacttta gaaggtgtta
     8461 ttgaggacct tgatgcagat ctaaaattag ttgaactttt acagccttac gccggtcgtc
     8521 tggctcgtga ccgtttttcc tatgaatcac ttaaagatac cgctacggag cacttttttg
     8581 aaactagcga ctttcttatt tccttaccac gtcgccttaa tgatctgtta gatcgtttgg
     8641 atacagaagg tctccccttt caaataaact accccgacat cgataaagct tttctgcatc
     8701 ttgaccgctt agcaaatcgc ttaacgttta gtattgtgtt attatccttt agtatcatta
     8761 tggcagcgct gattatcggt gccggcttta ttaccgccat cgccggtgaa acagccttac
     8821 tttggcgctt gccgattatg gagattggtt ttgtcgtcgc gacaggtatg gcaacctggt
     8881 tgattgccgc catctaccgc agcggtaaac tataagtgct taagcttttt tgcctcaacc
     8941 tgctaaaatg atgaatattt gtctgtcttt ttccctgttc taaataattg tcaggcttaa
     9001 taatattatc taggtggtta gctgttgccg ggatttaggg tcaacatttg ctgatagcaa
     9061 accgaaatgt ctaatttttc tgattattta ttgacaaaga agctgcttaa ttataatatg
     9121 gagataaagt aatattttcc agctagcagt ctggcagctt atcccacact cacctaactt
     9181 accttaggaa atgcaacagc gcctttagtc cctctatact aggtcgctgt tattattgtg
     9241 caagaaaagc gtgctcgttc aggttcctta acgcgcgctt ttcagtattg gctgcctctg
     9301 cagcctcgct gtacacagta tcctatccag tgcataaata atcttggagg tgtataaggt
     9361 gaaaaaaccg ttaagtccag ctctaaaaac attttatggt attggtgatt taggtttctc
     9421 cctgatgacg tctgtaggca tgtattttca aacgtatttt ttaacggatg ttgctggttt
     9481 taatttagcc ttggttggtc ttattatcac gatccctgga atttttgatg ccgtgtttgc
     9541 tccgatttat ggcgcaatca ttgatgctgt caaacctatg aagtggggta aatatcgttc
     9601 ctggctgatt cttacgcccc cccttgtggt tgtgctgatg acgatgacct acactaaaat
     9661 cggcactgat gccatggctg ctacaatcat cattgcttgc accctactgg ctcgaccttt
     9721 gtttaatatc ccctgggttg ccaacgtggc cctgatttca gtgctggcca ataatccgag
     9781 tgaaagagtt ttactttctt cccgcagagc tttttggacc agcttgtcac gggtgttttt
     9841 ctcatatatt gggcctcccc tggccgcata ctttggtggc agaacaaatc ctgttgctgg
     9901 ctactccatc ttacaatttc tgatggcgac aatgatgatg attggttatt ttattcaatt
     9961 taaaatgact gacggctacg aagaaacgac cagcaaaact gcgcaatcgg ctaaacaaac
    10021 ggcacaaaaa ccaaaagaga agacttcact taaagatttg ttaagcaacc ttttccaaaa
    10081 ccctccctta atggctttaa tgtttgctga ctttggccgt tacctcggta tgtttaccat
    10141 gaccgcctgt atcgcctact attttaccta tgtcgccgaa aatatggatt tgatgccacc
    10201 tttcatgctg attacagctt tggctggcgt ggcaggctca ctggccacac ccaccttaaa
    10261 taagaagctt aaaacacgtg gcacatccat cgttggttta ttgctggcag gcggcttaat
    10321 gttgttttcc aaaatcgtag gtttacaaac ctatatattt atggctatct gcactttagc
    10381 gcaatttttc atgggtatgt tggcttctac tctggtggcg ctttacagtg atacagtggt
    10441 ctatggtgaa tggaaaacag gaaaaaatac agccggtttt attatgggct taatgaacct
    10501 gcccctaaaa ataggttcat tagcacgtgg tgttgtcatg acagcagcct tggctggcat
    10561 tggctttatt gccggcatgg aaccgactct ggcactaaaa gatggcattc taaatattta
    10621 cgtagtcatg cccggtattg gcctgatgtt agctggcgtg atgttactgt tcggctttag
    10681 actcactgat gacaaactac aagaaatgac cgaggaaatt gagtctcgca aaggtaaagt
    10741 tattgtggaa cagtagcggc agccgactct tttcaaacga agactcgcac ataaagactg
    10801 caccgaagga taaaaccttt agtgcagtct tgcttatcta tgacctgcgt tttttatata
    10861 ctacaatttc tgcatctaca ccgcggtcac catactcaca aactaaaaga tagttttcac
    10921 tccccccccc aaaaaaatag acaccaagtc acaccgtatt tgtcttgctc attacctgaa
    10981 aaaagctaag cggagggatt atttttattc tgctaatgct gctttggctt tctgcagctg
    11041 ctgtgctaca gccacagcgg ctgtaccgcc acgggaagta cgggcagcca cacatgcttc
    11101 tggtgacaaa agttctaaag ccttatcagt aaataggggg ctgtgcgcct gcagctctgt
    11161 cgctgttaag tcactaaagg ggcgcttttc ggcaatacac caagcaatca gtctgcctac
    11221 gaccgcatgg gcttccctaa agggtaatcc cctccggaca aggtaatcgg ctaaatcagt
    11281 cgcgctggcg aaatcaccgg ctaccgctgc atacattctt tcttgattaa cacgcagcgt
    11341 ccgcagcatg gccgcataaa gagttaagca cattttaata gtatctactg tatcaaagag
    11401 cccctcctta tcttcctgca aatctttatt gtaggtcagg ggcaaaccct tcagcaccgt
    11461 taacatcgcc agcaggtgac cataaacacg accggtctta ccacgcacca gctccgccac
    11521 atcggggttt tttttctgcg gcataatact tgaccctgta gtaaaagcat catcaagctc
    11581 cacaaaacca aattcttgtg aattccataa aataaggtcc tcactgagac gacttaaatg
    11641 cgtcattaag agcgacgcat gagcgagaaa ctctaccacg aaatcgcgat cgctaactgc
    11701 gtccatacta ttttcataca gttggccaaa acctaagtcc tctgcgacca tctctcggtt
    11761 gatgggaaag gtggttcctg ctaaggcccc agaacctagg ggcatccagt cagcacgttg
    11821 ataagtctct agaaagcgct cctggtcccg ctgcagcttc caaaagtaag ctagcagatg
    11881 atgggcaaac aaaatgggct gggcccgctg caggtgggta taaccaggca taatcacatc
    11941 ctgatacgcc gccgctgttt gtagtaatac ctcttgtaag ttttttatta aagccaccgt
    12001 ctccaggcat tcttttttga cataaagatg caaatctagt gctacctgat cattacggga
    12061 acgagcagta tgcagttttc cccccaccgg accaataagc tcgattaatc gcttttccac
    12121 atgcatatga atatcttcca gtgccactgt aaattcgaat tcaccacgtt caatttctgc
    12181 ttgaatatct tgaagaccgt tgataattaa gtctgcttct gcttgggtaa taatcccaca
    12241 agctgccagc atgcggcagt gagcggtact accaccaata tcttccgcat aaagacgctg
    12301 gtcaaaaccg atggaggcat taaaagcctc catcagagca tctgtttctt tattaaagcg
    12361 tccaccccaa agtttcattt ttccacctac tttttctggc ttttagctaa gagtccttgt
    12421 acctttagtg gcagaccaaa taaatttata aacccttctg catcttgctg attgtataca
    12481 acatcttcac caaatgttgc caggtcttcc cggtaaagcg agaatggtga ggtacgtcct
    12541 gctactgtga tattcccttt gtataatttt atccgtacag taccggttac agtgcgctgc
    12601 gtgctatcaa agaaagcatc taaggcttcc cgcagcggtg aataccagag gccataatat
    12661 acaagctcac catatttgat ggctgccgtt tctttatagt gagcagtctc ccgatccaga
    12721 gttaaagctt ctaattcacg gtgagcaaag caaagtagtg tgccgcctgg tgtctcataa
    12781 atgccgcgcg acttcatccc taccaaacga ttctccacca tatccacacg gcccacccca
    12841 tggcgcccgg caatggtgtt caatgtcgtc aacagttcca ccggctctaa aatttgacca
    12901 ttaacactta agggaatccc ttgctcgaaa ccaatttcca catactctgg cgtctcaggt
    12961 gcttggctgg ggtcagtagt aagcataaac atctcttgat ctggttcatt atccggatcc
    13021 tccagaatac cgccttcata actcaaatgc cataggttac gatccatact ataaggttta
    13081 gccttcgtga cgggtacagg aatgccatgt ttggccgcat aatccatcgc gtcatcacgc
    13141 gatttaatct cccattcccg ccaaggtgca acaattttga gatgtggtgc cagtgctttt
    13201 acagttaatt caaagcgaac ttgatcattg cccttacccg tagcgccatg ggcaatggca
    13261 tctgcgcctt ctttttctgc aatttctact aaacgcttgg caattaccgg acgcgcaatg
    13321 gcagtgccca ataaatactt ttgttcataa atcgcccccg atttaatgta ggggaaaaca
    13381 aaatcacgca taaattcttc tctaatatct tcaatataga gcttgctggc accggaattg
    13441 atcgcttttt cctgcagcgg ctctaattct tcaccctgcc cgacatcggc agccatggca
    13501 acaacctcaa cattatattt ttctttcagc caggtaatgg caactgaagt atctaatccc
    13561 ccagaatacg ctaaaacaat ttttttcatt ttaatccctc cattttctca taagcactag
    13621 tataaagctt ttgcagtaat tgtcgggctg actctccacc actggttaac agctccacct
    13681 tactgctaac ttgctcttct tcacgtaaca aatccaaatt agccaaaacc cgtgtcaact
    13741 gtctgacggt acctagattg ccatcgataa agcttacgcc aagcggaaag atacgagtta
    13801 aagcttcttt aagaaataaa taatgggtac agcctaagac aacagtggct acggaactgg
    13861 tatcaatagc agcaaagatt tttactaact cttgctccac ttgtctgcct gctatgcagc
    13921 cctgctccac caattctacc agggttgggc acggcacaag cagcactttg gccaaatcaa
    13981 cttcacagcg ctgacaaaga tcatgaaact tttcttcttg cagcgttaac ggcgtagcca
    14041 ttaccagaac cttgccctgg tcaccatgtt ctaaagcagg ctttaccgcc ggctccatgc
    14101 caatcacagg aatcggcagc tgctcacgta ataaatgcac agccgcactg gtagcagtat
    14161 tgcaggctac caccaaagct ttacaaccac gcgccattaa aatttctgcc acctgcagac
    14221 ttaaggcctg cacttctgct ttacttttgc gaccatacgg tgcatgggct gtatccccat
    14281 aatagataaa atcttcatgc ggtaagcagt gaaccgctac agctaaaacc gagagcccac
    14341 ctacaccgga atcaaaaaag gcgattggtt tttctttttt ctctgctagt ttttgcatta
    14401 tccaccatca cactctctgc tatgataata gtttagccat tatggccttc tgtacatgca
    14461 gacgattttc tgcttgatca aacactaccg cttgcggacc ttccagcact tcttcactga
    14521 tttcttcacc gcggtgtgcc ggcaagcagt gcatcacgat agcctcgctt ttagcttgtg
    14581 ccagtaactg gttatttaat tggtatgatt taaagatctt tgctcggctc atttgctctg
    14641 cttcttgccc catactcgcc caaacgtctg tatatagcac atcagcatca gcagcggcag
    14701 ctgctggttc tgtcgtcaca gttactgtgg aaccatgctt ttgagcattg gcctgcgcgt
    14761 ctgcaacaat agcggctgac ggttgataac cgagggggct agccacggca acgtttatgc
    14821 cgagtttgct gcaggctagc agtagagaat gcgccatatt gttaccatca cccagataag
    14881 ccagtttcaa accgctaagt gtcccttgct gctcgagaat cgttagtaga tccgccatag
    14941 cctgacaggg atgtaataaa tctgttaagc cattaatcac gggtatctcg gcataagctg
    15001 ctaactcttc gacatcccgg tgggcgtagg tgcgaatcat aataccgtct agatagcggg
    15061 agagtacacg ggcagtatct ttaatttctt ctccccggcc aatttgcatg tctgctgcac
    15121 tcaagtataa agcataaccg cctaactgga acatccccgc ttcaaaagaa acacgggtac
    15181 gagtggaggc tttctgaaaa atcatcccta atgttttgcc tggcagtaag gcggcagctt
    15241 gacctgtcct ttgttttatt tttagctcct gtgcaaaagt aagtatttcc agcaattcat
    15301 ctggggtaaa atcattaacg cttaaaaaat cacgtccctg taaactactc attcggccac
    15361 atcctttctc ccgtgccttt gacccctttg tgccagataa tcctgtaaag caaccactgt
    15421 cggctgcagt tgaccagctg ttttgcgtgt taaaacctca aagaaagccc ttgcagtatc
    15481 cgtagaggta agacagggga tttggtattc cacacaagca cgccgcacct gataaccggt
    15541 agaagtaatt tttttaccat aggtaggagt attcactgct aaatcaattt taccttcacg
    15601 aattaaatct agcacattgg gcagaccttc acccacttta tttactgtct ccacgttggc
    15661 cgcacctgct ttctgcaaag cttgtgccgt ctcagccgtg gcataaatct taaaaccgcc
    15721 agcatgggct tgctgcagta aaggaataag ttcttgctta tctttgtccg cgacactggc
    15781 gagaatgttc cctctctgag gaaaggaaaa cccactggcc agcatacttt tgtacagagc
    15841 ggtggcaaag tcgtccgcca tacccaacac ttcgccggta gacttcatct ctggtcccag
    15901 cgagatgtcc agttgctgga gcttggcaaa ggaaaataca ggcgccttga ctgtaacata
    15961 tggtggtgca ggcactaaac catgcccata acccatttcc tgcagtgttt cccccaacat
    16021 gatcctcgtt gccaaagcca ccaggggcat acctgtgact ttgctaataa tcggtactgt
    16081 ccgactggcg cgaggattaa cttccaagac gtaaactcgc tcccgccaaa ccacatactg
    16141 aatattcacc aaaccacgga tccccaaggc taaagcaatg cgtactgtat attcagtaag
    16201 ggtctccatt aactcgggcg tgagtgattg cggcggaaaa acagccatgg aatcaccgga
    16261 atggacacct gctcgctcta tatgttcaaa gatgccggga attaacaccg tggtgccatc
    16321 acaaactgca tccacttcca cttcagtccc caacaaataa cgatctaata aaaccggatg
    16381 ctccggagtt acattggccg caagctgtaa ataagtataa agctgttctt cattataaac
    16441 aatctgcatg gcccggccgc caatcacgta agagggacgc accagtaaag ggaaacctat
    16501 tttatgtgct gctcccagtg cttcatgtgg tgaggagact gccacccctt ctggctgcgg
    16561 tatccctaac aagcgaagta actgattaaa cttctggcgg tcttccgcca agtcaatcgt
    16621 ctctagcggc gtgcccagca gtttgacgcc agcttgctgc aggggcccgg ccaaattaat
    16681 tgctgtttgt cccccaaatt gcacaaccac gcctagtggt ttttcttttt ccacgaccgc
    16741 taaaacatct tccaccgtca gaggatcaaa gtaaagttta tccgcacaat caaaatcggt
    16801 agagacagtt tctggattat tattaagaat tattgcttct cgtccactct ccttaagtgc
    16861 ccagacactg tgtacagaac aataatcaaa ctcgatcccc tggccaatac gaataggtcc
    16921 ggagcccagc actaacactt tttcctgctt actacagttc acctcatctt ctgcctgata
    16981 ggtagagtag taatagggca tcagcgtatc aaattcggcc gcacaggtgt ccacagtttt
    17041 gtaagctggt gccagggcca agcttttccg ctgggcacgc acggcctcgc tgctaacccc
    17101 caggatttta gccacctccg catcggcaaa acccaattct ttatagtgtc gcagttgctt
    17161 agccgtcagt gcagttaagt taagcttgcc tagtgctagc tcggcagcaa caatcttctt
    17221 gatcttgtca ataaaatagg gagcaatact actaatttct gcggcctccg ccacactacc
    17281 accccgacgc agcatttctg ccagcacaaa caagcgttca tcagtagggg cagccaattc
    17341 agcagcaagc tggcggtcac ttcgcgtttt taactctgcc atttgtaaac cagtcacacc
    17401 gatttccaga gagcggacag ctttaagcaa tgcttcttcc aacgtctgcc caatggccat
    17461 tacttcacct gtggccttca tttgtgtgcc tagttgtcga ttggcagctt gaaacttatc
    17521 aaaaggccaa cggggtattt tacagaccac ataatcaata gttggctcaa aacaagcagc
    17581 ggctttgccg gatccagagc ttaatatttc ctcaagctgt aaaccaaccg ctattttcgt
    17641 agcaacttta gcaatgggat aaccggtggc ttttgaggcc aaggcactag aacgactaac
    17701 acgcgggtta acctcaatta ctatatattt tttatctgta gggtggaggg cgaattgaat
    17761 gttacagcca ccttcaattt ttaatgcccg gataatcttt agtgccgcat tgcgcagcat
    17821 gtgctcctcc gtatcttcta aggtcagggc cggtgcaaag acaatggaat caccggtatg
    17881 cacacccaca ggatcaaaat tttccataga gcagatggtg atacagttat cctgagcatc
    17941 acgcattact tcaaactcaa tttccttcca gccggcgaca ctggtttcca aaagcacctg
    18001 cccaattaaa ctatgtttta ggccgcgaga aactatatcg cgcaaagatt ggtcatcagt
    18061 ggcaaaaccg ctgccagacc ctcccatagt ataggccgga cgaacaatga gtggatagcc
    18121 cactgcttgg gcaaagtcaa aggcttcctg caagctggag gagatgatac tcggagcagt
    18181 tggttctcca atctcctgca tagtttcttt aaatagttcc cggtcttcgg catgttttat
    18241 agtttccaaa gacgtgccca gcaggcgaac atgatatttt gccagcacac cagcttctgc
    18301 taactccttg gctaaattca aggccgtctg gccgcctaat gttggcagca agccgtctgg
    18361 acgctcccga gcaatgactt gtgagacaaa ctccagggag agcggttcaa gataaacccg
    18421 ctccgccaga tccggatcgg tcataatggt agccggattg ctgttaacca ggattacctg
    18481 gcagccttcc tcacgcaagg ctttgcaggc ttgggtcccg gcataatcaa attctgcggc
    18541 ttgcccgatg ataataggac cagaaccaat tactaatact cgttttaatg cttcatctct
    18601 cggcacagcc gggccccctt cttagtcaat tgctgttaac gtttgttcta aaatagttac
    18661 agcagcatca atttcttttt ctgttacaat gagcggcggc aagaaacgta atgttttacc
    18721 accaatacag ttaaccaata aacctttctc ctgacagagc gggaccaccc tggcagcatc
    18781 tccggcaatt tccaaaccaa tcattaagcc caggccgcgc acctcaacta ggtgagggta
    18841 agctagggct tgtagctttt gctgaaaata tgcccccagt tctgctaccc ggttcagaaa
    18901 atcagcttct gccaggatgc tcacagctac atttgctgcg gcacaaacaa ccggatttcc
    18961 cccaaaagtg gaggcgtgtt caccgggcac taacgttgtc gctgcttctc ctttggctgc
    19021 cattacccca atcggcagtc cgccgcctag tgctttcgcc agggtaaaga cgtctggttc
    19081 tacaccgtaa tgctgataag caaacaactt tcctgtacgt cccatcccgc attggacctc
    19141 atcaaagatg agcagcagct gatgtttatc acataactgt cgcagtccct gcagaaactc
    19201 caatgttgcg ggatagacac caccctcacc ttgaaccggc tcaattaaaa tggcgcaagt
    19261 ttgtgccgtg actgcatcct taaatgattg caagttatta aactcggcat agcgaaaacc
    19321 tgcaactagc ggttcaaagc ccttatgata tttgggttga ccggtagcag tcacggtagc
    19381 tagcgtccgg ccatgaaaag aacgcttggc ggtaataatt tcaaaggctg cctcaccctt
    19441 cttaagcttg ccatatttac gtgccagttt gaatgccgct tcattagctt cggcaccgct
    19501 gttgcagaaa aatactttat cagcacaaga gtgctgcacc aattgttctg ctaaacgaat
    19561 ctggggttcc gtgtagtaaa gattagaagt atgcatcaac tgttgggcct gtttataaat
    19621 ggcctcgaca atttgtggat gacagtgccc cagattgtta accgccaaac cactgacaaa
    19681 atctaaataa cgcttgccgg catcatccca aacataagca ccttcaccac gctgcagcaa
    19741 taaagtagct tcaggctgcc ggttataggt attaataatc gtcttttttt ctaatgcttt
    19801 aagttccatg gtctgcctcc taactcgtaa ccatggtacc aataccatga tccgtaaaga
    19861 tttccaacag taaagaatga ggaacacgcc cgtcaataat atgtgtacga tttacttcat
    19921 gttcaagcgc catgatacaa gcttcaactt taggaatcat acccttatta attttacctt
    19981 cagcaatcat ggccaaggct ttggctttag ggagggcaga aatcagtgaa gagggcacac
    20041 cagcctcgac aaaaatacct tccacatcag taagtaaaat caatttatca gccgccagtg
    20101 ctgctgccaa ttcaccggcc acgtgatcag cattaatgtt caggctttct ccttccagac
    20161 ccacagcaat cgaggaaatg accggtatgt agccttcttt acttaaagta tgaataacct
    20221 cagggttaac cttcgtgatt tcaccaacat agcctaggtc taccacttcc tcacttccgc
    20281 tcagctgctc cggagcacgg cggcgcgcca ccagcaaatt agcgtcttta cctgataaac
    20341 ctacagcttt gccgccatac tggttaatta aagcaacgat ttgtttgtta actttacccg
    20401 tcagcaccat ttctactact tcgatggctt ctttgtcggt tacacgtaaa ccattaacaa
    20461 actgtgactc ctgacctaaa cgctgcagca tttttgtgac ctctgctccc ccaccatgca
    20521 cgacaatggg gttaatccca atatacttaa gtaggataat atccatcatc actgattttt
    20581 ttaattcctc tgagaccatg gcatgaccgc catatttgat gacaaaagtt ttatcagcaa
    20641 aagaacgcaa ataaggcagc gattcaatta gtacatccgc tttggcaatt agctgctgaa
    20701 catcattgtt caccgatctt cactccttac acaaatttta gtcaccaagt taagacaaca
    20761 ctaggagcga taactggcgt taattttgat ataatcgtaa cttaaatcag ctccccaggc
    20821 agttactttt tcttcgccca tctgcaagtc aactgtaatg cgaatatctt gctgggctaa
    20881 gattgctttg gcttgtgcct catcaaaaag taaaccctga ccaaactgag tgacctgcaa
    20941 atcccccagg aagacatcaa cccgctctgg gaaaaactct acttccgttt gccccatggc
    21001 ggaaataatg cgtccccaat tagcatcctc accaaaaaag gctgttttca ccagattaga
    21061 gttgagaata ccgcgggcta aagttttcgc atcctcatag gtggcagcat gcttgactgt
    21121 tactgccaaa aacttggtgg caccctcacc atcgcgcaca atactctgtg ccagttccgt
    21181 gttgacccgc tgcaggagca tcagaaaacg atgataatct tcattctcct cactaattac
    21241 taagttagcg gctgccccat tagctaagac caccaccgta tcattggtgc tcgtatcacc
    21301 atctacggta atcaggttat aagaacgttc aaccgaatac tgcaaagcct tctgcagcag
    21361 cgcaggagca atggcgcaat cagtagtgat aaaagccaac atcgtagcca tattggggtg
    21421 aatcatgcct gaacctttag caataccacc cacggtaact atttctccgc caatatccag
    21481 ttgaaaagct gtgattttgg cagtaagatc tgtcgtcata atggcctcgg cggccagtgg
    21541 accgccctca gcttgtagct gctctaccgc gtcatgaata cccgtttcca gccgcgccat
    21601 gggcaggtac tgtccaatca caccggtgga ggccacaacg atgtcttgcg tctgtaggcc
    21661 caacagttca cccgtcagcg ctgccatacg ctgggcatcc ttaagcccct gctcgccggt
    21721 acaagcattg gcattgccgc tgttcacaat aatcgcctgt gctttgttat cagctagatg
    21781 ttcacggctg acaagtacag gagcaccttg gaaacggttc ttggtaaaca tcccagcggc
    21841 tactgccgga acttgagaat aaatcagcgc gatatctttt ttgtcctttt tcttaatgcc
    21901 agcagcgatg cctgccgcct taaaaccacg cgcggccgtc acgccaccct caactttacg
    21961 aaatttcatt actgctacct cctacttaag ccctgcttct tcgggccaat tctgcataat
    22021 attcatattt tgtaccgcct gacccgcagc gcctttgacc agattatcaa tggcagaaac
    22081 aactattaag cgtcccgtgc ggggatccag tcgaaaagct aaatcgcaaa aattggtgcc
    22141 gctgacatac ttcgtttccg gaagctcctg ggcggggagc agacgaataa agggctcctg
    22201 ggcataatgt ttttggtaag cagcgctcac ggttgcttca tctaccccag ccgcactacg
    22261 aacgtaaatg gtggaaagta tcccacgaat catcggtact aaatgtggtg taaaaattaa
    22321 ggtgacaggc ttaccggcca ggttactcaa ctcctgctca atttccggtg tatgctgatg
    22381 agcagcgatt ttataggcct taaaattttc tgtacactct gggaaatgga atgcttgttt
    22441 gggagaacga ccagctccag aaacacccga tttggcatca acaataatat cttctgcctc
    22501 aattaaaccc tcggcaagca gcggtttaag tgccagtaaa acactagtgg ggtaacagcc
    22561 cgggttagca atcagttctg catcccggat cattgcacgg ttgtattccg gcaagccata
    22621 aacgctaagt gctaggttct cctgactact atgttcatag ccataccagg tcggatacag
    22681 tgaagcatcc tttaaacgga aatcgccgct aaaatccact accttgatgc ctgtgtttaa
    22741 aagtttaggg acctctttaa ctgataaccc atggggcaag gcagcaaaaa taaaatcaca
    22801 ggccgccact tttgttaact ccagcttctc gagcggcaac tcacacaccg cacgaaactc
    22861 gggatagatc tgagcaaaac tctgcccact gaagctttca ctggtgatgt aatacggacg
    22921 ggcataggga tggtgagcca agatgctgac taactctttc cccgcatagc cagttgcacc
    22981 taaaacagcc acctttaaca ttataaacac
//
LOCUS       DASRMS010000122        18629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_768902, whole genome shotgun sequence.
ACCESSION   DASRMS010000122 DASRMS010000000
VERSION     DASRMS010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 18629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18629
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_768902"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..1591)
                     /locus_tag="VFC74_05700"
     CDS             complement(212..1591)
                     /locus_tag="VFC74_05700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014355910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK24862.1"
                     /translation="MKEEVQKIQQARGKLYSDFYNNIIPERMFVGMSVSSHILAEFGG
                     LDVFKVQYDYSQLAEPAKKMCDMIYSDTIPVRPVGLISRPPSFYQLLGSQSFVMGNRG
                     FVQHPEVVGMYEDEYPELIADPYSFLLEKVIPRQYKELDASNPVRMLNRMQMAKAALK
                     NDSASSMPWFLEMMDKHGYHKGAPPGSGGFSEAPYDFIADQLRSFSGISKDIRRHRSE
                     LKEACDAVLPLMFRMGLPANPHAEGSVMTPLHMPTFMREKDFMEVWMPSYKKMYEQYA
                     ALGIRGSAFCEDDWMRYLDNLLELPSGMVLMFEYGDPQQIKDKLGKKFIIQGLYPIDL
                     IKHGTKEQCVNKAKELLDIMMPGGGYVFGFDKGPLTLSDINLENLAAVAETVRDYGVY
                     ENAGESFGTPVNSEGFVFDESIASFKSKHLFNWDEFKAQYPLVPDSARANFEKYDKEL
                     FDLYMLMLI"
     gene            complement(1881..2360)
                     /locus_tag="VFC74_05705"
     CDS             complement(1881..2360)
                     /locus_tag="VFC74_05705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24863.1"
                     /translation="MTFPSALAQLTQLYTKKVQRVLHYQNLSAKFKGPDKMRGLITQL
                     IAEEREHLTMLSTVIVEMLSGEENDLAGKEAVTLAALGLELPPQLPLKPEETPDLAKA
                     IVTPGKPEEPAEPLPAVKATQPANHEQITTPQSKHSKYDFATAQKPLVWNFGRTPKH"
     gene            2663..3094
                     /locus_tag="VFC74_05710"
     CDS             2663..3094
                     /locus_tag="VFC74_05710"
                     /inference="COORDINATES: protein motif:HMM:NF037502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN family nuclease"
                     /protein_id="HZK24864.1"
                     /translation="MDGNCLEKDFISRTLHILKHYEGPYGVTLLVNCLLGLIVLPKEK
                     DYNHITERGQLHFSDLGIKETDVQWGELEDSKRTAAGLLRCLRNSVAHCQIESLSKDG
                     EIQALRFSDQRGFEAVFRLESLKQIIEKLAGYLDQLEIEKK"
     gene            3175..3783
                     /locus_tag="VFC74_05715"
     CDS             3175..3783
                     /locus_tag="VFC74_05715"
                     /inference="COORDINATES: protein motif:HMM:NF023166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiV family protein"
                     /protein_id="HZK24865.1"
                     /translation="MPQPFQAANIIEQSIQQAGITVAYPEVAGLSEQDMQAKINEQII
                     IVVRSMMLAQGITAVDLVQMEGTYTIELNEKGLLSIRFENYVRPDHRANGITLLRALT
                     FNLDTGVHYSFNELFKPSLPYHYLINNLIQEQIKQKALPTTGTVSSVTARQDFYLMRT
                     SVVIIFPELTFTPQVIGPPEFKLSFKQLRELVNPLGPLARLI"
     gene            complement(3859..4479)
                     /locus_tag="VFC74_05720"
     CDS             complement(3859..4479)
                     /locus_tag="VFC74_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014903733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HZK24866.1"
                     /translation="MSTENTTTAMQAASTLLIPSPPSQCNGQLYTVKAGDSLFFIGQR
                     FGVTVQQLQAANPQITNPNVIFIGQVICIPTGTPPPPDCEFRVLSLRILTENGQPIPR
                     TNNALQLPARVIVRATFTDPVQRAFFFLEPTGTGTCENARLIGIDCPSATTGVAEIIW
                     NVPMGTLGRVFVVACRNSVCAKSEEILVVRGNNNMTNTGTNNMNNM"
     gene            complement(4649..7870)
                     /gene="carB"
                     /locus_tag="VFC74_05725"
     CDS             complement(4649..7870)
                     /gene="carB"
                     /locus_tag="VFC74_05725"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HZK24867.1"
                     /translation="MPKQLKIKKVMVIGSGPIIIGQAAEFDYAGTQACRALQEEGLEV
                     VLVNSNPATIMTDTNIADRVYIEPLQLEPLTEIIAQERPDGLLPTLGGQVGLNAAKEL
                     AEKGILAQYNVQLLGTPLSAIIKAEDREHFKTTMQELQEPIPVSSIVSTVQDAADFAA
                     QNGYPVVIRPAYTLGGTGGGFAANDRELRLIAQQGLKASPIQQILVEQSVAGWKEIEF
                     EVMRDKNDTCITICSMENVDPVGVHTGDSIVVAPAQTLSSEDYTMLRSSALKTIRALG
                     IEGGCNVQFALDPDSSQYVVIEVNPRVSRSSALASKATGYPIAKVATKIAVGLTLAEI
                     QQTATPDNPASLEPAIGYVVVKVPRWPFDKFAFAQRTLGTQMKATGEVMAIADTFEAA
                     LLKAVRSLEIGAHSLHLDEVEKLSLHELKEKIAQPNDERIFMVAEALRRGVSIAELYQ
                     ISRLTPFFTQKIKNIIELEAEIKAYAGQDVDDFPLALWQRAKKMGFADATLAALLGTE
                     QTKVRTARLAAGIKPAYKIVDASAVGFTSASPYFYSTYAGKDEVLPTKRRKIVVLGSG
                     PIRIGQGVEFDYCSVHSVWALQDAGYETIIINNNPETVSTDFDTADRLYFEPLQIEDV
                     LNIIDKEQPAGVIVQFGGQTAINLAQPLVDAGVQIIGTSVDDIDRAEDRDRFDALLQE
                     LNLPRPAGHTVTSAKQAVPVAQRISYPVIVRPSYVLGGRAMEIVYNDQELAEYMSSAV
                     QVSPRHPVLVDKYLLGKELEVDAISDGHNVLIPGIMEHIERAGVHSGDSIAVYPAQSV
                     SAQHQQQIVDYTTRVARALHVKGIINIQYVLFEDNLYILEVNPRSSRTVPFLSKVTDT
                     PMVQYATHCALGQTLTSLGCTAGLLPAKPFVAVKVPVFSFAKLGDLETSLGPEMKSTG
                     EVMGIDKDLPRALFKALQAANLELPQTGQLLATVADKDKAEALPLLKRFVQLGFTLVA
                     TDGTAEVLEQVGCQVRRINKIAQGSPHVVDIIRGGEIDVVINTLTKGKKPERDGFRIR
                     RTAVELNIPCLTSLDTTKAILNVIESLITGGKVNYLPLQTYHQ"
     gene            8250..9161
                     /locus_tag="VFC74_05730"
     CDS             8250..9161
                     /locus_tag="VFC74_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK24868.1"
                     /translation="MKQERRMLLLADAALLTVAAIWGLTFVTVKNAINLLPPFAFNFY
                     RFGLAALIMAAFALPYRREVTKATVKAGTVLGIFLFLGYSFQTLGLLYTTASNAGFIT
                     GLSVVFVPLFSLLIAKERPASGVIIGALCAATGLAFISGVGVANFNVGDLLVLCGALS
                     FALHIIFVGKYTMIYSIVWLVTLQIGIVALCSGIFSLLLESGNNTFQPAVWPALIITA
                     LFATCFAFFAQNYLQQFTSPGHTAIILTSEPVFSAIFSVILLQETISSYAYWGGALIV
                     IGMILSEFKALPWSKTNEDSVPVTGQE"
     gene            complement(9319..10041)
                     /locus_tag="VFC74_05735"
     CDS             complement(9319..10041)
                     /locus_tag="VFC74_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZK24869.1"
                     /translation="MITVNELTKKFGTLEVLRGISCHIAPKEVVVVIGPSGSGKSTFL
                     RCLNQLEAATSGEIMIDGVSITDPKTNINMVRQEVGMVFQRFNLFPHMTALQNVALAP
                     IKVRKQKQQAAKDLALSLLERVGLADKADQYPEKLSGGQQQRVAIARALAMRPKIMLF
                     DEPTSALDPEMVGEVLEVMKSLALEGMTMAIVSHEMGFAREVGDRVLFMDEGLIVEEG
                     SPNQLFCNPTNPRTQSFLSKIL"
     gene            complement(10025..10696)
                     /locus_tag="VFC74_05740"
     CDS             complement(10025..10696)
                     /locus_tag="VFC74_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014905247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZK24870.1"
                     /translation="MDLRFNVVLDYLPMLIQGTKVTIQLTVMAVGIGTLIGLIVSLLR
                     LSGIKPLVLFAKIYTDFFRGTPLLVQIFLFYYGFPQLLGVQSFPRAISGIIALSLNSG
                     AYVAEIFRGGIQSIHRGQMEAARSLGMTSAQAMRYVVLPQAFKRCIPPLGNEFIAMLK
                     DSSLVSVISLQELMQTGRIIVGRSYRSFEPYIVVALIYLVMTLTISQFVSYLERVFGK
                     DDHGE"
     gene            complement(10790..11593)
                     /locus_tag="VFC74_05745"
     CDS             complement(10790..11593)
                     /locus_tag="VFC74_05745"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZK24871.1"
                     /translation="MKAWKKVVPVLMILLLAGLVGCGQSSKETAGTVQRFETMPPALL
                     ELTNRGIDAVVGDSPVVLEYIKSNPSAKLRAFPDDNFEKEFYGIAMRQEDTDLHKLVN
                     DGLAAIKANGVYDKLYAKYFGDGSDYVVPESENTLNLTYNVAQDMAYAPLESINAAGE
                     PEGFDCDLIRSIAAEMGFAVNLVNTNWDGIIPSLVSGTSDIIISAMTITEERQKSVTF
                     SDPYFEATQYIVVNEDSDINSLADLVGKTVGVQNATTGDIAITKYFEAQ"
     gene            complement(11932..12327)
                     /locus_tag="VFC74_05750"
     CDS             complement(11932..12327)
                     /locus_tag="VFC74_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HZK24872.1"
                     /translation="MEKDALAQFQDKVSELLIRHRSILDSLTKFQESSSRVNRSLVKS
                     VTHCGCLTISASKQYCPPEVDFKDCQKYMDSHLKGQLCESCREALEEELGNHLFYLAA
                     LCELLEIDLSEVFKDEFERVSTLGYFHLS"
     gene            complement(12546..12773)
                     /locus_tag="VFC74_05755"
     CDS             complement(12546..12773)
                     /locus_tag="VFC74_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24873.1"
                     /translation="MLKVYALDEIPDQERKSNNFDFHQTVTKPQKRYVPPMSHPWKAK
                     AFTDYVKAQKHHIAESQKAFEDIIYSQEIMF"
     gene            13223..13708
                     /locus_tag="VFC74_05760"
     CDS             13223..13708
                     /locus_tag="VFC74_05760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012065471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HZK24874.1"
                     /translation="MFSIGDKVVYPMHGAGVIEAIEEKEILGARKKYYIMKIPIGDMK
                     VMIPLDSVEQVGLREVIDIEGVKQVFTILKQDHSKMSTNWNRRYRANMEKIKSGDIFQ
                     VAEVFRNLMYRDKEKGLSTGERKMLDSAKQILVSELVLAQGKSEEDVDEILNRLLADV
                     S"
     gene            13911..15017
                     /locus_tag="VFC74_05765"
     CDS             13911..15017
                     /locus_tag="VFC74_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003447954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HZK24875.1"
                     /translation="MKPQLQRIVRIGALLIGFAIGYQLVNVSFSTIAAFAGMEATPVI
                     NVGASIFGGSIFGLIAYLISPWISKSMTTFNSWIETTLKAIPTQDIMVGVVGLVIGLL
                     IGALVSLALHRLPLIGSYLTVVVTLFTGYLGLSIMLNRKEEMAFLNNLFTRQARAAEK
                     ANFRSAKILDTSVVIDGRIIDITSTGFIEGVIIVPGFVLEELRHIADSSDTLKRNRGR
                     RGLDILNKIQKESLVPVQIVEQDFEDIMEVDSKLIKLAKTMKGKIVTNDYNLNKVCEL
                     QGVPVLNINELANAVKPVVLPGEEMVAQIIKDGKEVGQGVAYLDDGTMIVVEGGRRFI
                     GDTIEVVVTSVLQTAAGRMIFAKPKLAEKMSGVR"
     gene            15017..15703
                     /gene="ispD"
                     /locus_tag="VFC74_05770"
     CDS             15017..15703
                     /gene="ispD"
                     /locus_tag="VFC74_05770"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZK24876.1"
                     /translation="MSSALIIAAAGQGRRMKNQLNKQYLKLAGKPVVSYSLAAALAAN
                     CFTQIVVVVTPGEEELFRRDVLLPFFPEQELHVVTGGKERQDSVSNGLAVLVEKVTYV
                     CVHDGARPLAEAALFARCLKVAQARGAAIAAVRVKDTIKVVDDKGQVLTTPPREQLWA
                     VQTPQFFRRDWLELAHQRAKEEGFQGTDDASLLEHYGYPVQIIQASYENIKVTTPEDL
                     LLAEAVLGRR"
     gene            15705..16175
                     /gene="ispF"
                     /locus_tag="VFC74_05775"
     CDS             15705..16175
                     /gene="ispF"
                     /locus_tag="VFC74_05775"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004605166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HZK24877.1"
                     /translation="MRFGIGYDVHKLVADRPLILGGVTIPFDLGLLGHSDADVLVHAI
                     MDAMLGALALGDLGKHFPDTDPDYKGIASLQLLKQVAQLCRQQGYTIGNIDAIIIAQK
                     PKLAPYITSMRTNIAQTLEVTIEQISVKATTTEGLGFCGTSEGMAAQAAALLRA"
     gene            16503..17072
                     /locus_tag="VFC74_05780"
     CDS             16503..17072
                     /locus_tag="VFC74_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK24878.1"
                     /translation="MVITGENVVLALQAKRPEALEYLINQYGQSVYSLARTILRSAAS
                     EDVEECVSDIFAAAWQKSKEYDGQKGTIKTWLLMLTKYRALDYRRKLSTQSAYLASDE
                     QLTTSLQAVEDEVLQRERLQLTLAVVETFSELDQKLFFRRYFLQEALDELAAAFDLTK
                     RAVENRLWRCRKELRDQLAEKEGSSCGSS"
     gene            17059..18426
                     /locus_tag="VFC74_05785"
     CDS             17059..18426
                     /locus_tag="VFC74_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24879.1"
                     /translation="MDRHEQQLIKLLDLMDAKQIEKVLSVVDADGAKIVMPAVSVARI
                     KQQALGHAKLKKKSRPNKPLAFKLTAASLLLLVFAVAFYGPANTWAQIVKALNFIPGI
                     GKVVDNNKETTRHILAQPIELKLAESTLTVKAISVDQRNIMVVVEEVGDKLTLPTQAT
                     LVLGEQEYPLHRTSLSHQQRQENEHEAWTILDWESYFTRYGIVTAVEREGLVRVNYGE
                     EKQVEIPFKLEVAANFTDYAEMGPTASVKGLSVTAIPSLKDNLLKISLLSPPLAGQQV
                     RSYAYSLETLMTLSDQRGNSYPLAVKDHYSLPANEVLFDISGAETKQFILSIPCIVVK
                     QEKEAALLKLAIPPQGKTIVDQTVKLAGHEVILHTVERLDEKTIQLHVTLPQSDLARA
                     QNKDHLVDFDLKDYFNYGYGMHGDDTGRYYKINIPRDAEEISLTLKEPVLAIYGPWEI
                     ELDLE"
     gene            complement(18442..>18629)
                     /locus_tag="VFC74_05790"
     CDS             complement(18442..>18629)
                     /locus_tag="VFC74_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24880.1"
                     /translation="GSGLCLWLPSDSTSRWTPLPLANGSHCKAHSGLSPPSYRPCRAH
                     QKEKYAWPGILFINPNI"
BASE COUNT         5177 a         3841 c         4231 g         5380 t
ORIGIN      
        1 cattatgttc ctgctttttg tgggctaatc atattatact ttatccacag ctataattct
       61 atgaaaaagt attacaaaag cataaataag cagaatattt caaatactta ataactcagc
      121 ttgtttagcg ttggagtgta actttactta caaaaaagta taggcccgaa aggcctatac
      181 ttattgtata ttttgtactt tctttgtcga attaaatgag catcagcata tataagtcga
      241 atagctcttt gtcatatttc tcaaagttag ctctggcaga atcgggaacg agcggatatt
      301 gagctttaaa ctcatcccaa ttaaagagat gtttagattt aaagctggca atgctttcat
      361 caaagacaaa gccttcgcta ttaacgggag taccaaatga ttctccagcg ttttcataaa
      421 caccatagtc tctgacagtt tcggcaacag cggcgaggtt ttctaaattg atatcagata
      481 aggttaaagg acctttgtcg aaaccgaaga cgtagccgcc accgggcatc atgatatcaa
      541 gtaattcttt tgctttatta acgcattgtt ccttggtacc gtgtttgatc aaatcaatgg
      601 gatatagtcc ttgaataatg aactttttac ctagtttgtc tttaatctgt tgcggatcac
      661 cgtattcgaa cattaagacc atgccagatg gcagttcgag caggttgtcc agataacgca
      721 tccagtcatc ctcacagaag gctgagccgc ggatacctag agccgcatat tgctcataca
      781 tttttttata acttggcatc caaacttcca tgaagtcttt ctctctcatg aaggtaggca
      841 tatgtaatgg tgtcattacg ctgccttcgg catgggggtt agcgggtaag cccattctaa
      901 acatcaatgg caatactgca tcgcaggctt ctttcaattc tgaacgatgt cttctgatat
      961 ccttactgat accactgaag cttctcagtt ggtcagcgat aaaatcataa ggagcttcgg
     1021 aaaagccgcc ggagcctgga ggtgcacctt tatggtagcc atgcttgtcc atcatttcaa
     1081 ggaaccaagg catgctagag gcagagtcat ttttaagagc agctttggcc atttgcattc
     1141 tattgagcat tctaacaggg ttagatgcat ctaattcttt atactgccga ggaataactt
     1201 tttctaataa aaagctatat ggatctgcaa tgagttcagg atattcatct tcgtacatac
     1261 ctacaacttc agggtgttgc acaaagcctc tattacccat gacaaaggat tgtgaaccta
     1321 atagttgata gaaagacggc ggcctggaaa tcaaacctac cggtcttaca ggaatggtat
     1381 ccgaatagat catatcacac atttttttgg cgggttctgc tagctgtgaa taatcatact
     1441 ggactttaaa gacatctaaa ccaccaaatt cagctagaat gtggcttgaa actgacatac
     1501 caacaaacat tctttcggga attatgttgt tgtaaaagtc actgtaaagc ttgccgcgtg
     1561 cttgttgtat cttttgcact tcttctttca tttttaagct cccttcatta aaatattttg
     1621 ttctaggatt gttgtgctcc cattcaaact aaattatatc gtattttcga tttaagtcaa
     1681 ccaattaata agattataaa ttgtcagaat gtatcgaatc aaaaaatata aaggcagata
     1741 aactattttt tacaggcaga caactacgat gccctgccaa acatctatgc tgtgtattaa
     1801 agaaacaggc tgctctatga agcagcctgt tctgatactg acccagcagg tagcctgtaa
     1861 tgattagcgc aaacttttga tcaatgttta ggagtgcgcc caaaattcca cactagcggc
     1921 ttttgggcgg ttgcgaaatc atacttgctg tgtttgctct ggggtgtggt tatttgctcg
     1981 tggttagctg gttgagttgc ctttaccgcc ggaagtggtt ctgcaggttc ttctggtttt
     2041 cctggtgtga ctattgcttt agcaaggtct ggagtttctt caggcttgag gggaagttgg
     2101 ggtggcagtt ccagccctaa ggcagctaag gttactgctt cttttcctgc aaggtcattt
     2161 tcttcaccgc taagcatttc tacgatgact gtgcttaaca tcgtcaaatg ttcgcgttct
     2221 tctgctatta gctgcgtaat gaggcctctc attttgtcag gacctttgaa cttggcggat
     2281 agattttgat agtgaagcac acgctggact tttttagtat atagctgagt taactgagca
     2341 agtgcactcg gaaaagtcaa aagttatctc ctccttttgt ggcaaattta cgattctaca
     2401 cgggcaatct agtatatagt attactatgt attctcggat gtcaccattt gctatcaagt
     2461 aagataaaat agagatagaa gatcgtatac ccttatagct atcaagcagc ttccttgccc
     2521 ctacatagaa caaattagcc taacttacca gctaaacctt gggcatgcct gaaaggttct
     2581 taaaagcagt tcatcattct tgtaataata agtaaggatt gttgttaaaa ataaagggtg
     2641 aagactgtga tggaggtaca atttggacgg gaattgtctg gaaaaagact ttattagccg
     2701 tactttgcat attttaaagc actatgaggg tccgtacggt gtaaccttgt tagtaaactg
     2761 tttgttaggc ctgatagttt tgcctaaaga aaaagattat aaccatataa ctgaacgtgg
     2821 tcagcttcat ttctctgatt taggtatcaa agaaacagat gtgcagtggg gggagcttga
     2881 ggattctaaa agaactgcag caggcttgct gcgctgctta cgtaattcag tggcccactg
     2941 ccaaatcgaa agtttaagta aagatggtga aatccaagct ttacgtttta gcgatcaacg
     3001 cggtttcgaa gccgtgttta gacttgaaag cctgaaacaa attatagaaa aattggcggg
     3061 ttatcttgac caattagaga ttgagaaaaa gtagctagta ggccaacaga aatgtagcga
     3121 cattctgcac tactcttgaa tatgatagaa gaaagctatc aggaggagtg tattgtgccg
     3181 caaccatttc aggcagccaa cattatagag caaagcatac aacaagcagg cattactgtc
     3241 gcttaccccg aagtagcggg cttaagcgag caggatatgc aggctaaaat taatgagcaa
     3301 atcataattg tcgtacggag tatgatgttg gcacagggaa ttacagctgt tgatctagtg
     3361 cagatggaag ggacttatac catagaatta aatgaaaaag gacttttaag tatccgtttc
     3421 gaaaattatg tgagaccgga tcatcgtgct aacgggatca ctttgctcag agcattaacc
     3481 tttaatcttg ataccggagt gcactacagt tttaatgaac tatttaaacc aagtcttccc
     3541 tatcattatc tgatcaataa cttaatacag gagcagatta agcaaaaggc tttgcctacc
     3601 acggggacgg tgtcgagcgt tactgcccgg caagactttt atctcatgcg cacttccgtt
     3661 gtgattattt ttccagaact tacttttact ccgcaggtca ttggcccgcc cgaatttaag
     3721 ctatccttta agcagttgcg agagctggtt aaccccttag gcccactggc gcgattaatc
     3781 taattttttg ttgtttgcta agtgcagcaa gattagatca gaatatcttt atgtacacgg
     3841 gccggggccc gtgttttatt acatattgtt catgttgttt gtgcccgtat tagtcatgtt
     3901 attgttgccg cggacaacca aaatttcctc ggatttagca caaacactat tgcgacaggc
     3961 aactacaaag acgcgaccta aagtacccat gggtacattc cagattattt ccgcaactcc
     4021 tgtagtagca ctggggcaat ctataccaat tagacgagca ttttcgcaag taccggtacc
     4081 tgtgggctct aggaagaaga aggctctttg caccgggtct gtaaaagtag cacgtacaat
     4141 tacgcgtgca ggcagctgaa gggcgttatt agttctgggt atcggctgcc cattttccgt
     4201 aagaattctt aaggacaaaa cacgaaattc acaatcaggc ggtggcggcg tacccgttgg
     4261 gatacaaata acctgaccaa taaaaatcac attggggtta gtaatttgcg ggttagctgc
     4321 ttgcagctgc tgtacggtta ccccgaagcg ctggccaata aaaaaaagtg aatcacctgc
     4381 tttaacagta tataattgcc cgttgcattg tgagggggga gaagggatta gcaaggtgct
     4441 agcagcttgc atagcagttg tggtattttc agtactcaat tctttcacct ccttatgata
     4501 aaactactgc tatactatat gcaaaagggg cagggtttgt ttgaagacta gcaagaacta
     4561 ttgtccgcta gccatgaggg caaaaaaaaa ctccgacgtg tcagcggagt ttttttcgtg
     4621 atcagcatta gagtgcaaag gccaagcatt actggtggta tgtttgcagt ggcagataat
     4681 taactttacc gccggtaatt aagctttcta tcacattaag gatagcttta gtagtatcta
     4741 acgaagttaa gcaggggata ttcagttcta cagcggtacg gcggatacga aagccgtctc
     4801 tttctggttt cttacctttg gttaaggtgt taatgaccac atcaatttcc ccgccgcgaa
     4861 taatgtctac gacatgagga gagccttggg caattttatt aattctcctt acctgacatc
     4921 caacctgctc aagtacttct gctgtaccgt cagttgccac caaggtaaag ccaagctgga
     4981 caaagcgttt taagagggga agagcctctg ccttatcttt atctgccact gtggctaaaa
     5041 gttgacctgt ctgcggcagt tctagattgg ctgcctgcaa tgctttaaac agagcccgcg
     5101 gcaggtcctt atcaatgccc atcacttccc cggttgattt catttctggt cccagcgagg
     5161 tttctaaatc ccctaattta gcgaaagaaa agacaggaac tttaaccgcc acaaaaggtt
     5221 tagcgggcag taggccagct gtacaaccca gtgaagtaag tgtttggcca agagcacagt
     5281 gtgttgcata ctgcaccatg ggggtgtctg taactttaga taaaaaggga accgtacgcg
     5341 aggaccgcgg attgacttct aaaatataca gattatcttc aaacagcaca tactggatat
     5401 taatgattcc tttaacatgt agtgcccggg ccacgcgggt tgtataatcg acaatctgct
     5461 gttggtgttg tgctgatact gattgcgcag gatagacggc aatgctatcg ccggagtgta
     5521 ccccagctcg ctcaatgtgc tccataatgc cgggaataag cacattatgg ccatctgaaa
     5581 tagcgtctac ttctaattcc ttacccagca ggtacttatc taccagtacg ggatgacgag
     5641 gcgatacctg cactgcactg gacatatatt cagctagttc ttgatcatta tatacaatct
     5701 ccattgctct tccgcctagt acgtaggagg gacgaacaat tacgggatag ctaattcttt
     5761 gggcaaccgg gactgcttgt ttagccgaag taacagtatg tccagctgga cggggaagat
     5821 taagctcttg caagagagcg tcaaaacgat cgcgatcttc agcgcggtca atgtcgtcga
     5881 cgctagtacc aataatttgt acccctgcgt caactagtgg ctgtgctaaa ttaatggctg
     5941 tttgtccacc aaactgcaca atgacgcctg ccggctgttc tttatcaata atatttaaga
     6001 catcttctat ttgcagcggc tcaaaataca atctgtctgc cgtatcaaaa tctgtggaga
     6061 cagtttccgg attgttatta ataattatag tttcatatcc tgcgtcctgc agagcccaaa
     6121 ctgagtgaac agaacaatag tcgaattcaa caccttggcc gatacgaatg ggacctgatc
     6181 ccaacacgac tattttgcgg cgtttagttg gcagtacttc atctttacca gcataagtag
     6241 agtaaaagta aggacttgca gaggtaaaac caacagcact agcgtccact atcttatagg
     6301 ccggttttat gccggcagca aggcgtgctg tgcggacttt agtttgctct gtgcctaaca
     6361 gtgcagcgag agtagcatcg gcaaagccca tttttttagc cctttgccaa agtgctagtg
     6421 gaaaatcgtc cacatcttgg ccggcatagg cttttatttc cgcttccagt tcaataatat
     6481 tctttatttt ttgtgtaaaa aagggagtaa ggcgtgaaat ctgatatagt tcagcgatgg
     6541 aaacgccgcg ccgtaaagct tcagcgacca taaaaatacg ttcatcatta ggttgagcta
     6601 ttttttcttt taattcgtgt agagagagtt tttctacctc gtcgagatgc aaactatgcg
     6661 cccctatttc taaagagcgg acagctttta gcagcgcagc ttcgaaagta tctgcaatgg
     6721 ccattacttc accggtggcc ttcatctgtg tacccaaggt ccgttgggca aaggcaaact
     6781 tgtcaaaggg ccagcgaggt actttgacaa ccacatagcc aatggcaggt tccaaagagg
     6841 caggattgtc tggtgttgct gtttgttgaa tttcagccaa tgtcaagcct acggcgattt
     6901 tagtcgcaac tttagcaatg gggtaaccgg ttgctttgga ggctaaagcg gaagaacgcg
     6961 aaacgcgagg attcacttca atcactacgt actgtgagct atctgggtct agggcaaact
     7021 gcacattaca gcccccctca atcccgagtg cacgaattgt tttaagggct gaggaacgta
     7081 acatcgtata atcctctgat gataaggttt gtgctggagc aacgacaatg gaatccccgg
     7141 tgtgtacacc tacgggatcg acattttcca tactgcaaat ggtgatgcag gtatcatttt
     7201 tatcacgcat cacttcaaac tcaatttctt tccacccggc tacactttgt tcgactaata
     7261 tctgctgaat cgggctagcc tttaaacctt gctgggcaat cagccgtaat tcacgatcat
     7321 tagcggcaaa accgccacct gtaccgccta gggtataggc aggacggatg accacaggat
     7381 agccattttg tgcggcaaag tctgcagcgt cttgtacagt ggagacgatg ctggagaccg
     7441 gaattggttc ctgaagttcc tgcatggtgg tcttaaaatg ctcacggtcc tcagctttaa
     7501 taatggcaga gagcggtgtg cccagcagct gtacattata ctgcgcgaga ataccttttt
     7561 ccgctaattc ttttgctgca tttaggccaa cttgtccgcc taacgtgggc agcaggccat
     7621 ccggtcgctc ctgagcaata atttctgtta gcggttctag ctgcaacggc tcaatataaa
     7681 cacgatcagc gatattggta tcagtcataa ttgtggcggg attactattg accagtacga
     7741 cctcaagtcc ttcttcttgc agcgcacgac aggcttgggt gcccgcatag tcaaattcag
     7801 ctgcttggcc aataataata gggccagaac caatcaccat tacttttttg atctttagtt
     7861 gtttaggcaa gacaaaacaa ctccttctct gtgaggctgt tttttgatcg ttaatattat
     7921 ccgcaacaag ggagctggcc attaataaaa aggctaacca aacggcagct gcagccgtcc
     7981 tggttagcca aattgttgtt gttttagata cataaattta gtgcctcctt tttagtctct
     8041 ctggactaat ttaaaggtgc ctgtgaagtt cactcaattc tatcagaaga gtggcccggc
     8101 tgtcaagaac tgtgctctca gtttaacttg cattgactgc tttagtagtt acgaaaaacc
     8161 gtgacgagaa aagaagcttt tgccgtcgct agttttacag tttcatggta caataataag
     8221 atattattta actgtgagga gatagagatt tgaagcaaga gagacgtatg ttattactag
     8281 ccgacgctgc cttattaact gtagcagcta tttggggcct aacatttgta actgtaaaaa
     8341 acgcgatcaa tttattacca ccctttgctt ttaactttta tcgtttcggg ctggcagctt
     8401 tgatcatggc ggcttttgcc ttaccatata gaagagaagt aaccaaagca actgttaagg
     8461 ccgggacagt actggggata ttcctttttt taggatactc tttccagact ttaggtctgc
     8521 tttatactac ggcttccaat gccgggttta tcaccgggtt atctgttgta tttgtgccgc
     8581 tgtttagtct gctgatcgcc aaagagcgac ctgccagtgg ggtaattatc ggtgctctct
     8641 gtgcagccac tggtctggca ttcatttccg gagtcggtgt cgcaaatttt aatgtgggtg
     8701 atttattggt tttgtgcggt gctctttcct ttgccttgca cattatcttt gttggcaaat
     8761 atactatgat ttattcaatc gtctggctgg tgacactgca aatagggata gtagctttgt
     8821 gcagcgggat tttctcttta ctgctggagt caggtaacaa tacctttcag ccagccgtct
     8881 ggccggcttt aatcatcacc gccttatttg ctacctgttt tgctttcttt gcgcaaaact
     8941 atctgcagca gtttacttca ccaggtcata cagctattat cttaaccagc gaacctgttt
     9001 tttctgcaat cttttccgta atccttttgc aggaaacgat ctcatcgtat gcttactggg
     9061 gtggcgccct tattgtcatt ggtatgatct tgtctgaatt taaagcgctg ccgtggagta
     9121 aaaccaatga agatagtgtg ccggtgacag gtcaggagta agtcctttat tgtgcaggca
     9181 gcgtagtttg ctctgctcag aagcaatttt taagctagcc aaccgctaag ataagtaaaa
     9241 ggaacagcac aagctgctcc ttttcttttt tgccaagact aaatgcttgt ccataaagtt
     9301 aagctatcaa tagctccatt aaagaatttt actgaggaaa gattgtgtgc gtgggttggt
     9361 aggattacag aaaagttggt tgggagatcc ttcttctaca atcagtcctt cgtccatgaa
     9421 caagacacga tcccctactt cgcgggcaaa ccccatctca tgactgacaa tcgccatggt
     9481 cataccttct aaagccaagc ttttcattac ttccagcact tctcccacca tttccggatc
     9541 caaggctgag gtaggttcat caaaaagcat aattttcgga cgcatggcta atgcgcgggc
     9601 gatagcaact cgctgttgtt ggccgccgga gagtttctct gggtactgat ctgctttatc
     9661 tgctaacccc acccgttcaa gcagggataa tgctaaatct ttcgccgctt gctgtttttg
     9721 tttgcgtact ttgattggtg ctaaagcaac attttgcagt gccgtcatat gaggaaataa
     9781 attaaagcgt tgaaatacca tgcctacttc ctgccgtacc atattaatat ttgttttagg
     9841 gtcagtaata ctaacaccgt cgatcataat ctctcccgag gtggctgcct ctagttgatt
     9901 taggcagcgt aaaaaagtgc ttttgccaga accggagggg ccgatgacga cgacaacctc
     9961 tttaggagca atgtggcagg agatcccacg cagtacttct agggtgccga acttcttggt
    10021 cagctcattc accgtgatca tctttgccaa acaccctttc taaataggat acgaattgtg
    10081 agatagtcag agtcataacc aggtatatta aagcaactac aatatatggt tcaaaagagc
    10141 ggtaggaacg ccctacaatg attctgcctg tttgcatcaa ttcctgtaag gagatgaccg
    10201 aaactaatga ggagtctttt aacatggcaa taaactcgtt acctagtggc ggaatgcacc
    10261 gtttaaatgc ttgtggcaag acaacatagc gcattgcttg cgccgaagtc atgcccaggg
    10321 aacgggctgc ctccatttgt cccctatgaa tagattgaat cccgccacga aaaatctccg
    10381 ccacataagc accactgttg aggctgaggg caatgatgcc ggagatagct ctgggaaagg
    10441 actgcactcc tagtaattgc gggaagccgt aataaaataa aaagatctgt actaataatg
    10501 gtgtgccccg aaagaaatct gtataaatct tagcaaataa gacgagtggt ttaataccgg
    10561 ataaacgtaa aagactaact attaagccaa ttaacgtgcc aatgcctacg gccatcactg
    10621 ttagctgaat ggtaactttt gtaccttgga tgagcatggg cagataatca agcactacat
    10681 taaaacgaag atccattttc tccccccttt aaaaaattag tgggtaatgg tttgcattac
    10741 ccactaaaaa tataacattt aagtgtatag atgaacagac taaatcaggt tattgggcct
    10801 caaaatattt agtaatggca atgtcaccgg tggtagcgtt ctgcacacca actgttttgc
    10861 ccactaaatc tgccaaacta ttaatatcgg aatcctcatt tactacaata tactgtgtcg
    10921 cttcgaaata tgggtctgaa aaggtaacgc ttttttggcg ttcttcagtg atggtcatgg
    10981 cggagataat gatatctgaa gtaccgctta ctagtgacgg aatgatgcca tcccagttag
    11041 tgttcactag atttacagca aaacccatct cggcagcgat ggagcggatc agatcacaat
    11101 caaaaccttc aggctcgccc gcagcattaa tggactctaa tggagcatag gccatatctt
    11161 gggccacatt ataagttaga tttaaggtgt tttcgctctc tggcactacg taatctgagc
    11221 cgtcgccgaa atacttagca taaagtttat cataaacacc gttagccttg attgctgcta
    11281 acccatcatt tactagtttg tgcaggtcag tatcttcctg acgcatggca ataccataga
    11341 attctttctc aaaattgtca tcagggaagg cccgtaactt tgctgacgga ttacttttaa
    11401 tgtactctaa aactacaggt gaatcaccga caactgcgtc aataccacgg ttagtgagtt
    11461 ctaatagtgc tggcggcatc gtttcaaatc tctgtaccgt tccagctgtt tcctttgacg
    11521 attgaccaca acccacgagt ccggcaagca ataagatcat cagcaccggt acaacttttt
    11581 tccaagcttt cattttttta ccccctactt tagtacgttt tttggttaac acagtaaatt
    11641 cgatattaag ccttgttttc ctgcctagca ttagaaaaaa cagctgagga atttacagag
    11701 ctttgatatc agcttctcca atcataaaaa agttggcgtt tttagtttct gaacgcaatc
    11761 ttttgctctg ctccatgtac aatctgctac tagcacaaaa gagacgactg tgctcgtctc
    11821 ttaacgttag ttgcagaaat ggctgcatca atgtttactt gtatctttaa aatatcttct
    11881 agaatgaaaa acacgttaag caagcagccg aggttgatcc tgcagatttt gctaggataa
    11941 atgaaagtag ccgagggtag agacccgctc aaattcatct ttaaatactt cgctcagatc
    12001 gatctccagc agctcacaaa gagctgccag gtagaaaagg tggttaccta attcttcttc
    12061 aagcgcttcc cgacaagatt cacacagctg acccttaagg tgagagtcca tgtatttttg
    12121 acagtcttta aaatcaactt cgggggggca gtactgtttc gaagctgaaa ttgtcaggca
    12181 gccgcaatga gttactgatt ttacaagtga ccggtttact ctactagatg attcttgaaa
    12241 tttagtcagg ctatccagga tgcttcggtg tcggattaat aactctgata ctttatcctg
    12301 aaattgagct aaagcgtctt tctccacaga tatggccccc taactggttt tatgataatc
    12361 tctttttcat tatactgata ttggtaattc attgtcaatg taagttcatc atgaattcct
    12421 ttgctgcacg cttaaggcca aagaaaatgg tcaattttgg tgcgtataga ggtgtcaagc
    12481 atttttgatt atgtcccgaa aattttaaaa cattagcacc agcgtaagtt ggtgtttttt
    12541 tctgtctaaa acataatttc ctgtgaatag atgatatcct caaaagcttt ctgggactcc
    12601 gcaatgtggt gcttctgagc cttgacatag tctgtaaagg cctttgcctt ccaagggtga
    12661 ctcataggtg gcacgtaacg cttttgcggc ttggtgaccg tctgatggaa gtcaaagtta
    12721 ttagattttc tttcctggtc tggaatttca tcaagagcat atactttgag catatacttt
    12781 ctcccctacc gaagtgaata tgctgcagag cgaacttggc attgaatttc tcgatatagg
    12841 tggtcaagaa ttcatttgcc tgctctagcg tggtcacgcc tgctaaccgt aactcgattg
    12901 gtaggcgtga ttttaatgtt ccaaacatcc tctcaacccg gcctttagcc tctggtacgc
    12961 tagtagtctt gatgtcaacc cctagctgct tgcaggcgta gccaaattgt gtaaaggtat
    13021 ctttttcaag tttagactac actaccgaaa aagacataac tggtaatata tggtgggcaa
    13081 ttaaacttgc aattctcccg gaaactcaaa aaagcgtata gaggttgaca gagaaagtca
    13141 actgtgataa aataccttct atttgacaaa atacagcttg tatgttaaaa ttaaataacg
    13201 tcaacctagg aggtctgccg aattgtttag tataggtgat aaggttgtgt atccaatgca
    13261 cggcgccggt gttattgaag cgattgagga aaaggaaatc ctgggtgcgc gtaaaaaata
    13321 ttatattatg aagattccca ttggtgacat gaaggtgatg attcctttag atagtgtgga
    13381 gcaagtaggc ttaagagaag tgattgatat tgaaggtgta aaacaagtct ttacaattct
    13441 aaagcaagat catagtaaga tgtcaaccaa ctggaatcgt cgttatcgcg caaacatgga
    13501 aaaaatcaag agtggagaca tttttcaagt tgctgaagtt tttcgcaatc ttatgtatcg
    13561 tgataaagaa aaaggtcttt ccactggaga acgcaaaatg ctagatagtg caaaacaaat
    13621 tttagtatct gaacttgtac tggcacaagg aaaaagcgaa gaagatgttg atgaaattct
    13681 caatcggtta ctagcagatg tatcttaatt aaacaaggca tcatataatc ttattttatg
    13741 gtgccttttt tgtacttgct tcgcagttag caaagcttta gcagtcgcca gtacggcaga
    13801 gcttgagata atcaaatatg aacttttagt gaataatgta tcaatttgct tgaatctggt
    13861 aaaaactata cgttataata ggttaataaa tgaaaggagg tgctagatag ttgaaaccac
    13921 agttacagag aattgtgcgc atcggcgcgt tactgatagg ctttgcaatt ggctatcagt
    13981 tggtaaatgt cagcttttct actattgcag cctttgctgg catggaagca acacctgtga
    14041 ttaatgtagg tgcttctatt tttggcggtt ctatttttgg acttattgct tatcttatta
    14101 gcccctggat cagtaagtca atgactacct ttaatagttg gatagaaaca acattaaaag
    14161 cgatcccgac ccaggatatt atggttggtg tagtaggatt ggtcattggt ttactaattg
    14221 gtgccctggt tagtttagct ttacatcgac tgccattaat tggtagttac ttaacggttg
    14281 ttgtgacttt gtttacaggt tatttgggct taagtatcat gcttaaccga aaagaagaaa
    14341 tggctttttt gaataacttg tttacccgtc aggcccgggc agcggaaaaa gcaaatttcc
    14401 gtagtgccaa gattctcgat actagtgtgg ttattgatgg tcgcattatt gacatcactt
    14461 ccacagggtt tatcgaaggt gtgatcattg taccaggttt tgtacttgag gaattgcgcc
    14521 acattgcaga ttcctctgac actctcaaga gaaaccgtgg tcggcgtggt ttggatatcc
    14581 tcaacaaaat tcaaaaagaa tcactagttc ctgtacaaat tgtagaacaa gactttgagg
    14641 acatcatgga agtggatagt aagttgatca agctggccaa gaccatgaaa ggtaaaatcg
    14701 ttactaacga ctacaattta aataaagtct gtgaattaca aggtgtacct gtactaaata
    14761 ttaacgaact tgctaacgct gttaaaccgg tagttttgcc gggtgaggaa atggttgcgc
    14821 aaatcattaa ggatggtaag gaagttgggc agggcgtagc ttatctcgat gacggcacca
    14881 tgattgtggt tgaaggcgga cgccgcttta ttggtgatac cattgaagtc gtggttacca
    14941 gtgttttgca gacagctgca ggacggatga ttttcgctaa accaaagtta gcggaaaaaa
    15001 tgtccggagt taggtaatga gtagtgcctt aattatagca gcggctggac aaggccgacg
    15061 catgaaaaat cagctcaata agcagtattt aaagctggcc ggcaaaccgg ttgtaagtta
    15121 cagcctggca gcggcactgg ccgcaaattg ttttacccag attgtggtgg tagttactcc
    15181 cggcgaagaa gaactttttc gccgggatgt tcttttaccc ttctttccag aacaagagct
    15241 gcacgtggtg acaggaggaa aagaacgcca ggattctgtc agcaacggac tagcagtatt
    15301 agttgaaaag gtaacttatg tctgtgttca tgatggtgct cgtcccttgg cagaagctgc
    15361 cttattcgca aggtgcctaa aggtagcaca agctagaggt gctgctattg ctgccgtgcg
    15421 ggtgaaagat accattaaag ttgttgatga caaagggcaa gtgctgacaa caccgccacg
    15481 ggagcagtta tgggcagtgc aaacaccaca attctttcgt cgtgactggc tggagctggc
    15541 acatcagcgt gcaaaagagg agggctttca aggtactgac gatgcctctt tacttgagca
    15601 ttacggctac cctgtgcaga ttatccaggc cagctatgaa aatattaaag ttactacgcc
    15661 ggaagatttg ttgttggcag aagcagtttt agggaggcgt taagatgcgg tttggcatcg
    15721 gttatgatgt acataaattg gtggcagaca gaccactgat ccttggtgga gtaactatac
    15781 cttttgattt gggattgtta ggacattctg atgctgatgt gcttgtgcat gcgatcatgg
    15841 atgccatgtt gggagcgttg gccctgggtg atttaggcaa acatttcccc gatacagatc
    15901 cggactacaa aggcattgcc agtctgcagc ttttaaagca agtggcgcag ctttgccgtc
    15961 agcagggcta tacaatcggt aacattgatg ccatcattat tgcgcaaaaa ccgaaactag
    16021 caccatatat cacatcaatg cgtaccaata ttgctcaaac ccttgaggta acaattgagc
    16081 aaattagtgt caaggccact accacggaag gtttgggctt ctgcggcaca agtgaaggca
    16141 tggccgccca agctgccgct ctcttgagag cgtgacaggg gacggttctt tgacacggaa
    16201 attcatccgc attttattct tctttgctca acatagctta aaaagtgcac gatggttttt
    16261 taatgtgcgt tatctagtag ctttatcaaa ggtaagcctt tctaggactg tttttgttgc
    16321 gattgactta aacatctgat acagaatagg caaccttttt tatttgtaca aaaatattgt
    16381 tgggcatgat tcactgggtt aagacctgtg gcagtgcgac aaagaaccgt cccctgtcgc
    16441 gactgagagt tttagctatc acatgcgtag taattagtag aaggcgggaa aggagaatgc
    16501 caatggtgat tacaggagag aatgttgtgc tggctttaca ggcaaaaagg ccagaggcac
    16561 tagaatatct aatcaatcaa tatggtcaat ccgtttactc cttagcccgt actattctcc
    16621 gcagcgccgc ctctgaagat gtggaggaat gtgtcagtga tatttttgct gctgcctggc
    16681 agaaaagtaa ggaatatgat gggcaaaaag gcaccataaa aacctggctg ctaatgctga
    16741 caaaatatcg agcgttagat taccgtcgca aactttctac ccagtcagct tatctagcga
    16801 gcgatgagca gctgacaaca tctcttcagg cggtagagga tgaggtgctg caaagagaac
    16861 gtttacagct gacactggcg gttgtagaga cttttagcga gttggatcaa aagctattct
    16921 ttagacgcta cttcctacag gaagcactgg atgaattggc agctgctttt gacttaacca
    16981 agagagctgt tgaaaacaga ttatggcgct gccgcaaaga gcttagagac cagcttgcgg
    17041 aaaaggaggg gagcagttgt ggatcgtcat gaacagcagc tgataaagtt gcttgattta
    17101 atggatgcta agcaaataga aaaggttttg tctgtagtag atgcagatgg tgccaagatc
    17161 gtgatgcccg ctgtttccgt ggccaggatt aagcagcaag ccttgggcca cgcaaagtta
    17221 aaaaagaaat caagaccaaa taaaccactg gctttcaaac taactgccgc ctcgctactt
    17281 ttattggtct ttgctgtggc tttctacggg ccagccaata cctgggcgca aattgttaaa
    17341 gctttaaatt ttattcccgg tatcggtaaa gttgttgaca ataataagga aacaacacgc
    17401 catatacttg cacagccaat tgaactaaag cttgccgaga gtacactgac agtaaaagcg
    17461 ataagcgtgg atcaacgtaa cataatggtt gtggtagagg aagttggtga caagcttacc
    17521 ctgcctacgc aggctaccct tgttttaggt gagcaggagt atcccttaca tcgtacttca
    17581 ctgtcgcatc aacaaaggca ggaaaacgag cacgaagcat ggactatcct ggattgggaa
    17641 agctatttta ctcgctatgg cattgtcacc gcagttgagc gggaaggtct tgtcagggtg
    17701 aattatgggg aggaaaaaca ggtagagatc cccttcaagc tggaggtagc agctaatttt
    17761 actgattatg ctgagatggg gccaacagca tctgttaagg gccttagtgt tacagccatt
    17821 ccctctttaa aggataattt gttaaagatc agcctgcttt ctccgccttt agctggtcag
    17881 caagtgcgca gctatgcata ctctctagag acgctgatga cattatcaga tcaaaggggg
    17941 aatagctatc ctctggccgt taaagatcac tactctctgc ctgccaatga agttttgttt
    18001 gatatcagtg gggcggagac caaacaattc atcttgtcaa ttccctgcat agttgttaag
    18061 caggaaaaag aagcagcctt gcttaaactg gccattcctc cgcagggcaa aacaatcgtt
    18121 gaccagacag tcaaacttgc cggtcatgag gtaattttgc acacagttga acgtttagat
    18181 gaaaaaacaa ttcagctgca tgttacgctg ccgcagtctg atttggcccg cgcccaaaat
    18241 aaggaccatt tagtggattt tgacctcaag gattatttca actatgggta cgggatgcat
    18301 ggtgatgata caggcaggta ctataaaata aacatcccta gggatgctga agaaatttct
    18361 ctcacgctga aagagccagt gctagcaatt tatgggccat gggagattga gctggatcta
    18421 gagtaatcag cattagcttt gctagatgtt ggggttaata aaaagtatgc ctggccaggc
    18481 atacttttct ttttggtgtg cccggcatgg gcggtagctt ggcggtgaaa gtccgctatg
    18541 ggctttgcag tgggaaccat tagccaaagg caagggtgtc catcgtgagg tggaatctga
    18601 aggaagccag aggcaaagtc ccgacccga
//
LOCUS       DASRMS010000123         1651 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_769446, whole genome shotgun sequence.
ACCESSION   DASRMS010000123 DASRMS010000000
VERSION     DASRMS010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1651)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1651)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1651
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_769446"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..>1651
                     /locus_tag="VFC74_05795"
     CDS             91..>1651
                     /locus_tag="VFC74_05795"
                     /inference="COORDINATES: protein motif:HMM:NF024548.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HZK24881.1"
                     /translation="MTKKTLPTLLVAIVMVLVAACNTHDDLLVNQPQQTRTLTLTATM
                     PHDGPTTRVALEKKDDNSIALTWQDGDELQLLFVQNDIKEKAIATIKNITEEGKKAQF
                     DIVLPTNINDGNFTLYGIYGGGGLDDANPAIVKLPANPGSATSLADVQTRKDVMLYFK
                     STNIDAAKPTAAVSFEHLGSLLNITLKNKSTTPLTNIKEVRLVGTGGDGKWAYNHNQG
                     AQTYNLLTGEFENKGSAGNYLSFAAPSNSLAGDAEITFWGWNPPLPDKNWPELKLELW
                     GETTVTSITANSKPARGSAIAAGRAFYFYAIWDGSKVSFTDSSFTPEYFIEEGGLDAA
                     LGDDKDNIDAIALRGSVNSADFEVMKSMPSLRHLDLSGVTVAGNKIPDYAFGAAPAAG
                     MGAKNNTPKTSNTNATHTANTTIETVVLPDNITAIGNYAFAQCTNLKGNVTIPNSVIS
                     IGDGAFENCNKLDGTLTLPESLETIGARAFQFCKFNSQLILPSNLVSIGEEAFGWCDK
                     LYGDLTIPNTVT"
BASE COUNT          544 a          429 c          337 g          341 t
ORIGIN      
        1 cctttatagc ataaaagata caaccgtaac aaaacaacag aacaaataac aattttataa
       61 ccataaccca tatttaaaac aaccataaat atgacaaaga aaacattacc cacactatta
      121 gtcgccattg taatggtgct cgttgctgca tgcaacaccc acgacgactt gctggtaaac
      181 caaccacagc agacacgcac actaaccctc acggctacca tgccccacga cggaccaacc
      241 acaagagttg ccctagaaaa aaaagacgac aactccattg ccctcacatg gcaagatggc
      301 gatgagttgc aactactatt tgtgcaaaac gatattaaag agaaggcaat tgccactata
      361 aaaaacatta ccgaagaggg caagaaagca cagttcgaca tcgtacttcc cacaaatatc
      421 aacgacggaa acttcaccct atatggcata tatggtggtg gcggattgga cgatgcaaac
      481 cctgccatag taaaactacc tgccaaccct ggcagcgcca cctcgctagc agatgtgcaa
      541 accaggaaag atgtaatgct ctattttaag tcaaccaata tagatgctgc caagcccaca
      601 gccgcagtgt cgtttgagca ccttggttcg ctactaaaca tcacactaaa aaacaaatct
      661 accaccccac taaccaacat aaaagaggtg cgcctagtgg gcactggcgg cgatggcaaa
      721 tgggcataca accacaacca aggagcccaa acctacaacc tgctcactgg cgagtttgaa
      781 aacaaaggct cggctggcaa ctacctctct tttgctgcac caagcaacag cctggctggc
      841 gatgcagaaa ttaccttttg gggatggaat ccacccctac ccgataaaaa ttggccagaa
      901 ttaaaactag aactatgggg cgaaaccacc gtaacctcta tcactgccaa cagcaagcca
      961 gcaagaggca gcgccatagc agccggcagg gcattctatt tctatgctat atgggatggc
     1021 tcgaaggtga gctttacaga tagttccttc acccccgaat actttataga agagggtgga
     1081 ttggacgcag ctctgggcga tgacaaagac aatatagatg caatagcttt aaggggctcg
     1141 gtaaacagtg ccgactttga agtaatgaaa agcatgccat cgttgcgtca cctagatttg
     1201 agcggtgtaa ccgttgcagg caacaaaata cccgactatg cttttggagc tgctcctgca
     1261 gcagggatgg gtgcgaaaaa caacaccccc aaaacaagca acaccaacgc cacacacacc
     1321 gccaacacca caatagagac agtggtgcta cccgataata ttacagccat tggcaactat
     1381 gcatttgccc agtgtaccaa tttaaaaggc aatgtaacca tccccaacag cgttatatcc
     1441 attggcgatg gagctttcga aaattgcaac aagctcgatg gcaccctcac cctccccgag
     1501 agtttagaaa ctattggggc aagggcattt cagttttgca aattcaatag ccaactcatt
     1561 cttcctagca atttagtttc gataggagaa gaggcgtttg gttggtgcga caagctatat
     1621 ggcgatttaa ctataccaaa cacagtaaca t
//
LOCUS       DASRMS010000124         1450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_569280, whole genome shotgun sequence.
ACCESSION   DASRMS010000124 DASRMS010000000
VERSION     DASRMS010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1450
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_569280"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..244
                     /locus_tag="VFC74_05800"
     CDS             <1..244
                     /locus_tag="VFC74_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24882.1"
                     /translation="VAYKQRCSEKSFRLSAGLLAFAIAGFNLACVGYLVLIQPNHADL
                     MDYVHIVASTVLLVIVMRFKGNTSRMIDWVLNKPAG"
     gene            complement(248..1198)
                     /locus_tag="VFC74_05805"
     CDS             complement(248..1198)
                     /locus_tag="VFC74_05805"
                     /inference="COORDINATES: protein motif:HMM:NF022019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZK24883.1"
                     /translation="MAKTQTFIEKARAVHGDNYSYAETSYTKAHEKVIINCSDHGSFE
                     QSANDHLKGYGCAKCGKESRAAKIRQKAKDVFIEKARAKHGDRYGYDRAVYVYSHERV
                     IITCKKHGDFEQTPNHHLKGRGCAKCGFEEVAAKNTQEAKDRFIEKACAMHGDKYDYL
                     KTVYVRALDKVIITCKIHGDFEQTPNCHLRGNGCPSCPARYDQPTNLYLMHNGLGKVK
                     IGYSIDTNRRITELNSNAPFEAELLETWTLTDTPTVRKIESKVHRKLEKYHAGLSGFN
                     GATEWFNASPDTARKHILDAIRGSHVVKNYVKNNPQMSLI"
BASE COUNT          347 a          333 c          319 g          451 t
ORIGIN      
        1 tgttgcgtac aagcagcgct gcagtgagaa aagctttagg ctgtctgctg gacttctggc
       61 atttgctatt gcaggcttca atcttgcttg cgttggctat ctggtactga tacagccaaa
      121 tcatgcggat cttatggatt acgtgcatat cgtggcaagc acggtgctgc tagtgatcgt
      181 tatgcggttt aagggtaaca cttcgcgaat gattgattgg gtattaaaca agccagcggg
      241 gtaaaaatca tattagcgac atctgaggat tatttttaac ataattcttt acaacatggc
      301 tgcctctaat agcatctagt atgtgctttc ttgctgtatc agggcttgcg ttaaaccact
      361 cggtagcacc gttaaagcct gataaaccag catggtactt ttccagctta cggtgaactt
      421 tactttctat tttgcgcacc gttggtgtat cggtcagcgt ccacgtttct agcagttcag
      481 cttcgaatgg ggcgttgctg tttagctctg ttatgcgtct attcgtgtca atggaataac
      541 caatctttac tttcccaagg ccattgtgca tcaggtatag gttggtgggc tggtcgtagc
      601 gcgctgggca tgatgggcag ccgttacccc ttaaatggca gtttggtgtc tgctcaaaat
      661 cgccatgtat tttgcaggtg atgataacct tgtcgagcgc tcggacataa acagtcttta
      721 aataatcgta cttatcgcca tgcatagcac aggctttctc gataaaccta tctttagcct
      781 cttgtgtgtt ctttgctgcc acttcctcaa atccgcattt agcacaacct ctacctttta
      841 aatggtggtt tggtgtctgt tcaaaatcac cgtgcttttt acaggtgata ataacccttt
      901 cgtgcgaata aacataaacc gcacggtcat aaccgtaacg atcgccatgc ttggcacgtg
      961 ctttctcaat aaaaacatcc ttggcctttt gtcttatctt tgctgccctt gactctttgc
     1021 cacactttgc gcagccgtag ccttttaaat ggtcgtttgc actctgctca aatgaaccgt
     1081 gatcactgca atttataatt actttctcgt gcgctttagt gtaagaggtt tctgcataag
     1141 aataattatc accatgaacg gcacgcgctt tctcaataaa ggtttgtgtc tttgccatat
     1201 cagatacctt ggaacaaagg gaacatgagg aaatgcggct ggtgggcgtt caactccacc
     1261 acggcttgca aaccgttgcc gcactgtcaa ttataacaca taaaaaagcc agcgggttag
     1321 gctggcttgt ggtggttatg aagaaatata tgctggcatc gtatctgata ccaccgctgg
     1381 ctttcttggt tttatataat tctccagatc cacacaaccg ccaatcaccc gatcaccatc
     1441 cgtgataatc
//
LOCUS       DASRMS010000125         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_165131, whole genome shotgun sequence.
ACCESSION   DASRMS010000125 DASRMS010000000
VERSION     DASRMS010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165131"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..544
                     /locus_tag="VFC74_05810"
     CDS             <1..544
                     /locus_tag="VFC74_05810"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZK24884.1"
                     /translation="GSKYSLAENIALTRRVAEVAHAMGASIEGELGKIGGTEDDISVD
                     ERDTLFTDPAEALQFVEEAQLDALAVAIGTAHGPYKGEPILDFDRLAVINERVATPLV
                     LHGASGVPDSSIRQAVELGIQKINIDTELRQAFTRGVQQVIQAKPGEFDPRKILGPAK
                     EEMKAIVKTKMRLFGCSGKA"
     gene            558..1202
                     /gene="fsa"
                     /locus_tag="VFC74_05815"
     CDS             558..1202
                     /gene="fsa"
                     /locus_tag="VFC74_05815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012303236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HZK24885.1"
                     /translation="MKLFLDTANLQEIKQAMAWGVIDGVTTNPTLVAKEGGDFMALLK
                     EICQLVPGPVSAEVIATETSAMIAEAEKLAAVAPQVVIKVPMTPAGLTAVAALRSQGI
                     RCNVTLVFSFNQALLAAKAGAAYVSPFLGRLDDLGHDGVELVSEIYSIFRHYDYPCEV
                     IAASIRHPLHVLQVAQAGAHIATIPFKVLKQMFKHPLTDLGIERFLADWQKTQG"
     gene            1449..2432
                     /gene="glpX"
                     /locus_tag="VFC74_05820"
     CDS             1449..2432
                     /gene="glpX"
                     /locus_tag="VFC74_05820"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HZK24886.1"
                     /translation="MERELALEFVRITEAAALAAGRLMGRGDKEAADQAAVDAMRSVF
                     DTVYVDGTVVIGEGEMDEAPMLYIGEKVGIGKEPSVDIAVDPLEGTNLVAKGLPNALA
                     VVAVAPQGCLLHAPDIYMDKIAVGPQAKGCVDLDASVTENLQAVAKALNKDVQDVTAI
                     ILERPRHAAIIEEIRAAGARVKLITDGDVSAAIATALPNTGVDILLGIGGAPEGVIAA
                     AALKCLGGEIQARLTPDGEEELTRVRKMGQADPHKLLQLDDLVRGDDVFFAATGITDG
                     DMLRGVRYRGQTAETHSLVMRGITGTIRTIDATHLLSRKPHFVARHQDCEA"
     gene            2880..3836
                     /locus_tag="VFC74_05825"
     CDS             2880..3836
                     /locus_tag="VFC74_05825"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZK24887.1"
                     /translation="MKMSPNSTLAGVLVSLLILLPTNGEAATVPVVADAPIDVPSESA
                     FRPAYFTELGEVREQLKAQEAAILQHTIKRGETLSTIAKKYGLSVKSLVQTNNLSNPN
                     FVYEGQVLTLITNAEAAKNYTAPVIHRLQRGETIWELAKRYQTDVDDILAANNIDNAK
                     KLEPGLTLTIPGAAMPVSQTPNRLIASRSTAQATNTGFVWPTKGYISSGYGPRWGKFH
                     YGLDIAANTGTPLVAIAAGRITDSGWRQGYGYMVRIDHQNGWVSVYGHCSKLYVKGGQ
                     QVRRGQQIAAIGQTGNATGPHVHLEMLKKGKHHNPINYLPAR"
     gene            3941..4138
                     /gene="rpmE"
                     /locus_tag="VFC74_05830"
     CDS             3941..4138
                     /gene="rpmE"
                     /locus_tag="VFC74_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015263800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HZK24888.1"
                     /translation="MKEKIHPNYQQTSVTCACGNTFETGSTKKDLKVDICSNCHPFFT
                     GKQKFIDTGGRVERFKRKYGM"
     gene            4252..>4860
                     /locus_tag="VFC74_05835"
     CDS             4252..>4860
                     /locus_tag="VFC74_05835"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZK24889.1"
                     /translation="MSAAPVVGGQAVIEGVMMRHKNRIAIAVRKPDQQLSLYEETLTT
                     FGEKYPLFKLPLLRGMVAFIESLVLGTRALTISATEAALEEEEELTGWQLTLTVGAAL
                     LFGVGLFFFLPTVVMRLLAGNSTAPPLILNLLEGVLRLTIFLAYLLIVSRLQDIQRVF
                     EYHGAEHKSVACFEAGEELTVVNARKYSTLHPRCGTSFLLLVM"
BASE COUNT         1299 a         1069 c         1245 g         1247 t
ORIGIN      
        1 cggttctaaa tactccctag cagaaaatat tgccttaacc cgcagagtgg ctgaggtggc
       61 acatgccatg ggtgcttcga ttgaggggga acttggcaaa attggtggca cggaagacga
      121 tatttctgtg gatgaacgtg ataccttatt tacagatccg gcagaagcgc tgcagtttgt
      181 ggaagaagcc caactggatg ctttggcggt ggcaatcggg acagctcacg gcccttataa
      241 aggcgagcca attttagact ttgaccgctt ggctgtaatt aatgagcgtg tggcaacccc
      301 attggtgctg catggtgctt caggagtgcc cgacagcagt attcgtcaag ctgtagaact
      361 tggtattcaa aaaattaata ttgatacaga gttgcggcaa gcttttacac gtggtgtgca
      421 gcaagttatt caagctaagc ctggtgaatt tgacccccgt aaaatccttg gtccagctaa
      481 agaagaaatg aaagcgattg tgaagactaa aatgcgtctc tttggttgtt ctggaaaagc
      541 ataggagtga tcataacttg aaattatttc ttgacaccgc caacttgcag gagattaagc
      601 aggcgatggc ctggggtgtc attgacggcg tgaccactaa tcccacactg gtggccaaag
      661 aaggcggcga ctttatggca ctgctcaaag aaatttgcca gttggtgcca ggcccagtgt
      721 ctgctgaagt aattgccact gaaactagcg cgatgattgc tgaagcggaa aaactggcag
      781 cggtggcacc acaggttgtg attaaagtac ccatgacgcc agctggcctt acagcagtgg
      841 cggctttaag atcacaagga atacgttgta acgtaacctt ggttttctcc tttaaccaag
      901 cgctcttagc cgcaaaagcc ggggcagcct atgttagtcc ctttctcggc aggctcgatg
      961 acttgggtca tgacggcgta gaattagtgt ctgagatcta cagcattttt cgtcactatg
     1021 actatccctg cgaagtgata gctgccagta ttcggcatcc tctgcatgtg ctgcaggtag
     1081 cacaagccgg tgcccatatc gccacgattc cgtttaaggt acttaagcaa atgtttaagc
     1141 acccgctgac tgacttgggc atagaacgtt ttctggcaga ttggcaaaaa actcaaggat
     1201 aactacaggg cgtctgcagg cgcccttttt aatgtttggt aaaataatta gtacacaaaa
     1261 aacgtgtaaa aagtgctata atattatttg agcgcaagca attaaccttg acataagctt
     1321 tgtttgtcag gccattgttg ctggcatacc actacgcagg gaggaagcta gtgttatcag
     1381 cgctgtcagc gggagttaag ggaatatttc cgtgtctctt aaataaccta gagggggagt
     1441 ttgttctatt ggaaagagaa ctggctttag aatttgttag aataacggag gcggccgctt
     1501 tagctgcggg gcgtttaatg ggacgtggtg ataaagaagc tgccgaccaa gcagccgtag
     1561 atgctatgcg atccgtcttt gatacagttt atgttgacgg tacggtagta attggcgaag
     1621 gggaaatgga tgaagcaccg atgctttata ttggagaaaa agtaggtatc ggcaaggagc
     1681 ccagcgtgga cattgcagtg gatccgctgg agggaactaa tttagttgcc aagggcttac
     1741 caaatgcgct ggccgtagtc gcggtagcac cgcagggctg cctcttacac gccccggata
     1801 tttatatgga taagattgct gtgggtccgc aagccaaggg ctgcgtggac ttggatgcta
     1861 gtgtgacaga gaatctgcaa gcggtagcta aagccctcaa taaggatgta caggacgtca
     1921 ctgctattat tttagaacgt cctcgccatg ctgccattat cgaagaaatt cgtgccgctg
     1981 gtgcacgggt gaaactaatc actgatggtg atgtttccgc ggccattgct acagctttgc
     2041 cgaacacggg tgtagatatt ttgctgggca tcggtggtgc tcctgaaggt gtgatcgctg
     2101 cggcagcgct gaaatgcctc ggcggagaaa ttcaagctcg tctcactcca gacggcgaag
     2161 aagagctgac cagagtacgc aaaatggggc aggccgatcc ccacaaattg ttacagctag
     2221 atgacctggt acgcggtgat gatgttttct ttgctgctac cggcattacc gatggtgata
     2281 tgctgcgtgg cgtccgctac cgcgggcaaa cggctgaaac gcattcttta gtcatgcgtg
     2341 gtatcaccgg caccatccgt acgattgatg ccacccacct gctctctcgt aagccgcatt
     2401 ttgttgctcg tcatcaagat tgcgaagcat agatttaagc taaatcacga atcaaggaac
     2461 agtctggcca tgtcaaggct ggttccttga tttttttgtg ctgtgctcgg ccttaacagc
     2521 tgctgaaaac acagtactat cagcaaggat aagcaaaact tacctttcta acatctgtgg
     2581 cccaaacgag agaaaatttt tatcaagctc taaaaaagta gatgatgaag ccgctaactt
     2641 tacccggctt gactaaatat gtataaaatt taacaattgg ggaagactta cctagaccgt
     2701 gtttttcctg gaaaacgttg tggcgacaca gcgttttcgc tcctttttga ctggcagaga
     2761 aataactact gcgcccttag cgaagtaatg cgacagccag gagaaaattg ctactcggaa
     2821 actgcaagca caagcttgat gcttaaagct gacgtgctac gtaaagaggt gaataataaa
     2881 tgaagatgtc cccaaatagc acgctggcag gtgtactggt atctctcttg attctgctgc
     2941 cgaccaatgg tgaggctgcc accgtaccgg ttgtggctga tgccccaatc gatgtaccct
     3001 cagagagtgc ttttcgacct gcctatttta cagaacttgg tgaagtgcgt gagcagctca
     3061 aggcccaaga ggctgctatc ttgcagcaca cgattaaacg tggtgaaaca ttaagtacga
     3121 ttgctaaaaa gtatggtctg agtgtcaaat cattggtgca gactaataat ttgtccaatc
     3181 ccaatttcgt ttatgaagga caagttctca cgctgattac caatgccgag gcggcaaaaa
     3241 actatactgc tcccgtgatc catcgactac agcgcggtga aaccatctgg gaattagcca
     3301 aacgttatca aactgatgtg gatgatattt tggcagctaa taacattgac aatgctaaaa
     3361 agctagaacc ggggctgaca ttgactattc ccggtgctgc aatgccggtc tcacagacac
     3421 ctaatcgcct gatcgcttca cggtcaactg cacaagctac taataccggc tttgtctggc
     3481 cgactaaagg ctatatctcc tcaggctatg ggccgcgctg gggtaaattc cactatggac
     3541 tagatattgc cgccaacacg ggcacaccgc ttgtggcgat tgccgcagga agaataaccg
     3601 attccggctg gcgtcagggc tacggttata tggtcagaat tgaccatcag aatggctggg
     3661 tttctgttta cggtcactgc tccaagcttt atgtcaaggg cggccaacag gtacgacgcg
     3721 gacagcaaat tgcggccatt ggtcaaacag gtaatgctac cggtccccat gtgcatctgg
     3781 aaatgctgaa aaaaggtaag catcataatc ccattaatta tctgccggca cgctaattct
     3841 gcacgaaggt cacaatattt tcttaaaaaa tcttgcactg gtgaacaccc tatgttaata
     3901 tagtagatgt ggatttttat ggcggaaaga ggtgaacacc gtgaaagaaa aaatacatcc
     3961 taattaccaa caaacaagcg tgacctgtgc ctgcggtaat acgtttgaaa ctggttcgac
     4021 aaaaaaggat cttaaagttg atatctgctc caattgccac cccttcttta cagggaaaca
     4081 aaagtttatt gacaccggtg gacgggtaga acgctttaag cgtaaatacg gcatgtaact
     4141 acagacaggc ggcattaaag cctgaaaatt tgatttgaaa aaaacagcag cgggtaacgc
     4201 tgctcttttt tactatttaa aaaaatatgg ggagaaacga ggggaaatat tatgtctgct
     4261 gcccctgtag tcggtggtca ggcagtcatt gaaggtgtga tgatgcgcca taaaaaccgc
     4321 atcgccatcg ctgtgcgcaa acctgaccag cagctatcat tatatgaaga aacactaacg
     4381 acgtttggtg agaaatatcc gctttttaaa ttacccctgc tgcgtggcat ggtggctttt
     4441 atagaatcgc tggtgctggg gacaagggcg ctgacgattt ctgccacgga agcagccctg
     4501 gaggaagaag aagaattaac gggttggcaa ttaaccctga cggtaggtgc agcactgctt
     4561 ttcggtgtgg gactattctt ttttctgcct actgtggtga tgcgtttgtt ggccggtaat
     4621 agtacagctc cacccctgat cttaaatctc ctggagggcg tactgcgtct gacgatcttt
     4681 ttagcatatt tgctgattgt ctcccgtctg caagatattc aacgcgtctt cgaatatcat
     4741 ggggctgaac ataaatcggt agcttgtttt gaagccgggg aagaactaac agtcgttaat
     4801 gcccgtaaat attctacact gcatccccgc tgcggcacga gctttctttt gctggtgatg
//
LOCUS       DASRMS010000126         3410 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_435150, whole genome shotgun sequence.
ACCESSION   DASRMS010000126 DASRMS010000000
VERSION     DASRMS010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_435150"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..1500
                     /gene="murC"
                     /locus_tag="VFC74_05840"
     CDS             130..1500
                     /gene="murC"
                     /locus_tag="VFC74_05840"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HZK24890.1"
                     /translation="MLKDIKHIHFIGIGGYGMSALARVLLDIGYIVSGSDKKQSAITA
                     MLQKEGAVVYQDHRGTNITGAELVIYSTAIAPDNVELVAAKQAKLPVWHRSELLAQFI
                     NGRFGIAIAGAHGKTTTTSMVAAVLTRGQLDPTAFIGGIFHEFNGNSRIGKSELVVAE
                     ADESDNTFLRYRPRIAVVTNIDADHLEHYQGDFRLLLNAYREFLNNVAADGTAVLCAD
                     DPYLRDMRPTHLRKVVTYGLEEGDWRAENLQEEGWRTRFTVIGPTGMHGEIALPVPGR
                     HNVLNALAAVAVAQELKVDFSAIREGLESFHGADRRFQILCQENDIVVVDDYAHHPTE
                     IRATIQATRAGHNKRLLIVFQPHRFSRTKCFFNEFVQAFAGADKVFLQQIYAASEKPL
                     EGISSGKLAEEMRARGMDVTQLDRAEDIIEQVLAMAQPGDLIITMGAGDVTEIGHKIA
                     ESLQTR"
     gene            1509..1727
                     /locus_tag="VFC74_05845"
     CDS             1509..1727
                     /locus_tag="VFC74_05845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24891.1"
                     /translation="MSKQWTQQFNVPVIPQPCVPDGLAADIWGVMPNTPAHAAGLHPA
                     DLVLAVNQQLMRTRVEAFQLLKLYKIPY"
     gene            1878..2168
                     /locus_tag="VFC74_05850"
     CDS             1878..2168
                     /locus_tag="VFC74_05850"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HZK24892.1"
                     /translation="MASEFAHSLLQQVVTKFKLLSVVVKPVPNHYFGGSIKAAGLLTV
                     EDLLIAAREALSSRQYDLLLVPREAFDGHQLDLTGQPLHKLADASSLPVLAV"
     gene            2194..2685
                     /locus_tag="VFC74_05855"
     CDS             2194..2685
                     /locus_tag="VFC74_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013986710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HZK24893.1"
                     /translation="MNYVRVFVEVPKGSRNKYEFNKTTGRFLLDRMLFSSVHYPADYG
                     FIPETLAEDGDELDALVLAGDPTFPGCEIRAVPVGVLEMWDEKGKDEKVICVSLRDPV
                     WGWITEYDHLPPNLLREISHFFKIYKDLEDKITRIGEWFGKEEAWAVIRASRERYANR
                     REV"
     gene            2785..3366
                     /locus_tag="VFC74_05860"
     CDS             2785..3366
                     /locus_tag="VFC74_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24894.1"
                     /translation="MEAIILDNIPFTAELEMFTERLRIQKRPAMIEQFAELLEEAKGI
                     ARPKVMFKEMPIAEKGENFIVLDGVTFHSRELRHTVDSLPRVFPTVVTCGLELGNWSA
                     AYEDMMVRFFAEVICEGAMTSAQEYLAQYAAQQHGAEAITYILPGTLDWPIEEQEKVF
                     TLVGDTTPIGVHLTAQGTMRPIKSVSALYYETV"
BASE COUNT          924 a          691 c          851 g          944 t
ORIGIN      
        1 catcttcgga gctatattgc gttgattcga cagaaagctg gcaaaaatac tgtcatgaac
       61 cactagttca ctaataatat ttttaacata aactaaaaaa gtgagaatct atggaaagag
      121 gggtcgcctt tgctaaagga catcaaacat attcatttta ttggtatcgg cggttatggg
      181 atgagcgctc tggcacgtgt cttactggat attggttaca tagtcagcgg ctctgataaa
      241 aaacaaagtg ctattactgc aatgctgcaa aaagaaggtg ctgtagttta tcaggaccat
      301 aggggtacta atatcaccgg cgcagaacta gttatttatt caacagcgat tgccccggat
      361 aatgtggaac ttgtcgctgc taaacaggct aagctgcctg tctggcatcg ttcagaatta
      421 ctggcacaat tcattaatgg ccgctttggc attgccattg caggggcaca tggcaaaact
      481 acgactactt ctatggtggc cgcagtatta acccgtgggc aacttgatcc cactgctttt
      541 attggcggta tctttcatga atttaatggg aattcacgaa ttggtaaatc tgagcttgtg
      601 gtagctgaag ctgatgaaag cgataacaca tttttgcgtt accgtcctcg tatcgctgtt
      661 gtcaccaata ttgatgccga tcatcttgaa cattatcaag gtgactttcg tctgctgctt
      721 aatgcttatc gagaattttt aaataatgtt gctgctgatg gtacagctgt actctgtgcg
      781 gatgatcctt atctccgtga catgcggccc acccatctac gaaaagttgt tacttatggt
      841 cttgaagaag gtgattggcg tgccgagaat ctgcaggagg agggctggcg aactcgcttt
      901 acagttatag ggccgacagg aatgcacgga gaaattgcct taccagtacc tggccgccat
      961 aatgttttaa acgccttggc cgcagtagct gtagctcagg aacttaaagt agatttctct
     1021 gccatcaggg aaggtttaga gtcctttcat ggtgcagaca gacgttttca aatcctgtgt
     1081 caggaaaatg acattgtggt ggttgatgat tacgcacatc acccgacgga aatacgggct
     1141 actattcagg ctacacgtgc tggtcataat aaacgactac ttattgtttt ccaaccgcac
     1201 cgttttagtc gaaccaagtg tttttttaac gagtttgttc aagcttttgc gggcgccgac
     1261 aaagtatttc tacaacaaat ttatgctgct agtgaaaagc ctctagaggg catttcttca
     1321 ggcaaattag cagaagagat gcgtgctcgt gggatggatg tgacacaact tgatcgggcc
     1381 gaagatatta ttgagcaagt gttagccatg gctcaacctg gcgatttaat tatcactatg
     1441 ggtgccggtg atgttactga gataggtcac aaaatcgcgg aaagtctgca aacgcgataa
     1501 tatgagtaat gagcaagcag tggacacaac agtttaacgt accagtaatc ccgcagccct
     1561 gtgtgccgga tggtttagca gcggacattt ggggagtgat gcccaataca cctgctcacg
     1621 cagcaggtct gcaccccgct gatttagttt tagccgttaa tcagcagctg atgcgcacac
     1681 gtgtggaggc ttttcaactg ctaaagcttt acaaaatccc ttactgacaa ttaagcggtg
     1741 ggtacaaatc actaaacatc agttatctaa agcagctcac caacccccag ggtttgttgt
     1801 attatatgat tttgttccta ccgggtcgaa gagatagctg cggcgattaa acgttttcgc
     1861 gctgtgcatg tactattata gcttctgaat ttgcacattc cctactgcag caggtggtaa
     1921 ctaaatttaa gcttttgtct gtggtggtga agcccgtgcc caatcactat tttggcggat
     1981 caattaaagc agccgggctg ttaacggtag aggatttact cattgccgct agggaggcct
     2041 tgtcctccag acagtatgat ttgctgctgg tgcctcgtga agcgtttgat gggcatcagt
     2101 tagatttaac tggacaacct ctacataaat tggccgatgc ttcatccctg ccagtactgg
     2161 cggtgtaaaa tatacttgaa gaggagcacg caaatgaatt atgtgagagt gtttgtggaa
     2221 gtacctaaag ggtcgcgcaa taaatatgag tttaataaga caacaggacg ttttctctta
     2281 gacagaatgc ttttttcctc tgtccattat cctgcagatt atggctttat tccggaaaca
     2341 ttggccgaag atggggacga gctcgacgcc ttagtgctgg ccggagatcc caccttccca
     2401 ggttgtgaaa tccgtgccgt ccctgtgggg gttctggaaa tgtgggatga gaagggaaag
     2461 gacgaaaaag tcatttgtgt ttcgctgcga gaccccgtgt ggggctggat tacagagtat
     2521 gatcatctcc ctcctaatct gctgcgagag atctctcatt tctttaaaat ttataaagat
     2581 ttagaggata aaataacacg cattggtgag tggttcggta aggaggaagc atgggcagta
     2641 atccgcgctt cacgtgagcg ctatgctaat cggcgtgaag tttaacaagg ggctgcccgg
     2701 ccctggagca ggacttttat agttgagcgc gaaatgtaaa gcatagcaac gtaagagagt
     2761 aacttattct tcaggaggta ggatatggaa gctattattc ttgataatat tccctttaca
     2821 gccgaattag agatgtttac agaaaggctg agaatccaaa aaagacctgc gatgattgag
     2881 caatttgctg aactgctgga ggaggccaaa ggtattgctc gtcctaaagt gatgtttaaa
     2941 gaaatgccga tagcagaaaa aggtgaaaac tttatcgtgt tggatggtgt gactttccat
     3001 agccgcgagt tgcgtcatac tgttgattca ttaccgcgtg ttttcccgac ggtagttact
     3061 tgtgggcttg aattaggcaa ttggtctgca gcctacgaag atatgatggt gcgttttttc
     3121 gcagaagtta tttgcgaggg ggcgatgact tcggcgcagg agtatttggc acagtatgca
     3181 gcacagcagc atggtgctga ggccatcacc tatatactac caggaacact ggactggccg
     3241 atagaagaac aagagaaggt ttttacacta gtaggtgaca caacccctat tggggtacat
     3301 ctaactgcac aaggcaccat gcgtccaata aaatctgttt ccgcacttta ttatgaaacg
     3361 gtataagatc acgaaacgta aagaggctgg gacaaaactc agtctaaatg
//
LOCUS       DASRMS010000127         1603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_846182, whole genome shotgun sequence.
ACCESSION   DASRMS010000127 DASRMS010000000
VERSION     DASRMS010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1603
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846182"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            624..1421
                     /locus_tag="VFC74_05865"
     CDS             624..1421
                     /locus_tag="VFC74_05865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24895.1"
                     /translation="MTTITNYTKKQKNAFIKKHDLANLLPELDKAITFDLDQLLHRNS
                     AEINRTLRIAALRSESDRLTELGQQVLHYVLAVVEGVKWDNGRIALGKKEVTIKTDLV
                     FTVYIEEAKAKAEKDAKDKAEKAEKEKAEKAEKAKAAAQEKAEKAAAAKAKADDNKAD
                     EVAQAAAATAIFEAKAADSKLIAGDRNMSAKTLQNALHSLIAGNAKFSTLQDINDTFK
                     LLQLAQQALARYAKAVPNVDVDEDRVEELSKTKPTGKEKTAPKKKTG"
     gene            1473..1595
                     /locus_tag="VFC74_05870"
     CDS             1473..1595
                     /locus_tag="VFC74_05870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24896.1"
                     /translation="MKTDKIKALEEKLDRLEQLLKQLHSEADALAKTKASRMAS"
BASE COUNT          535 a          370 c          369 g          329 t
ORIGIN      
        1 tttggaaaaa tctgaaaaat ttctggcggg gtacgagggg ccttcccaga ccaggcatag
       61 ctaaaaatta tgggcggtgt ctggctgata gaaaagacta atcagtcgct tgacctgccg
      121 accgatagac aaaactaatc aaccttgctc aaccctatag aattattcaa ttgtacacat
      181 tctcgcagct cgctataatg gacacatcaa aaggggaagc actacagccc ctagcattac
      241 gcgcccttgc ccgttttgcg cccgttcttt aataatttag tgatagcaga tagctttgct
      301 catgtcgtgc gcttgtgtgc agactgttta aagcaactgg cgaacccgcg gcgcgtcaat
      361 tttttgacgt gtaaaacatc gtgccgtcaa ttttttgaca ctagcaccag aataatcgcg
      421 tagttttgtc cttttatatc gtgctatcgc ttaggccagc ctatggcctt ttggctaata
      481 cccaatatcc gccatgcgga tagctcctta acaattcaga acaaaatgac aagcgcaaag
      541 cattccggct ttgcgcgtag gttataactg ccagcaagtt attaatagct tgctggttta
      601 tataacttat tggagtaaag actatgacaa ccatcaccaa ctacaccaag aaacaaaaga
      661 atgcctttat caaaaagcac gacttagcga acttgctacc tgagcttgat aaggctatca
      721 ctttcgacct agaccaactg ctgcaccgta acagcgcaga gattaaccga accttacgga
      781 tcgctgcgtt gcgcagtgag tcggatagac taactgagtt agggcaacaa gtgctgcact
      841 atgtgctggc agtagtcgag ggcgtaaagt gggacaacgg cagaatagca ctaggcaaaa
      901 aagaagtcac aatcaagact gatttagtgt tcaccgtata catagaagag gcaaaagcaa
      961 aagcggaaaa ggatgcaaaa gacaaagcgg aaaaagcgga aaaagaaaaa gcggaaaaag
     1021 cggaaaaggc taaagcagca gcgcaagaaa aagcggaaaa agcagcagca gcaaaagcaa
     1081 aagcggacga caataaagcg gacgaagtag cgcaagcagc agcagccacg gcaattttcg
     1141 aggcaaaagc agccgatagt aagctaattg ctggtgaccg taacatgagc gccaaaacgc
     1201 tgcaaaatgc cttgcatagc ctgattgctg gtaatgctaa gtttagcacc ttgcaagata
     1261 ttaacgacac tttcaagctt ctacagcttg cccagcaagc actagcacgg tatgcaaagg
     1321 ccgtaccgaa tgtggacgta gacgaagacc gcgttgagga actatcaaaa accaaaccaa
     1381 cgggcaagga aaaaacagca ccgaaaaaga aaaccggcta aacccacttc tagcagcgca
     1441 ttaacgcgct gctacctacc ttggagaaca ttatgaagac agataaaatc aaagcactag
     1501 aggaaaaatt agaccgcttg gagcagcttc tgaagcagct tcatagcgag gccgatgctc
     1561 tggccaaaac caaggccagc agaatggcca gctaaaccta atc
//
LOCUS       DASRMS010000128         1128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_99352, whole genome shotgun sequence.
ACCESSION   DASRMS010000128 DASRMS010000000
VERSION     DASRMS010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1128
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99352"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            796..>1128
                     /locus_tag="VFC74_05875"
     CDS             796..>1128
                     /locus_tag="VFC74_05875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24897.1"
                     /translation="MGTLKNYIKEVTGVELTSKQLGRLHALALKASADEKKTVQEKYG
                     IDEDFALVVIEWLEYKKERRESYKSPRSIKAFITRLQNLSGNDLEKAKRIIQISIANN
                     WAGIFELKE"
BASE COUNT          415 a          253 c          243 g          217 t
ORIGIN      
        1 aattaaaggg gtccaatcgg agcccccagg ctatctcata ttcggaactg ataggcctaa
       61 gcgggctaga gaccagcgag ctcaacaccc agctgaacaa tttatacaga atcggaatga
      121 tatccgttta taaagcgatt aacgacaaaa tgataacttt aagaaccccc accaaatgag
      181 acgagtaagc aaaaaacaag cccagcgaaa tagggagcta gcaaaaataa aaagtgaaat
      241 agacaagaac tgctgtatct gcgggcgcca cggttatcag ctcatgcacc tgctcccacg
      301 gagcctttat cctgaacact acaccaggaa agagaaccta gcaatgggct gtcagatttg
      361 ccacaaccag tacgataaca acctggagta caggcgccag caaactcatt tatttaaaag
      421 gatttgcaaa tttgatgagc caggcgctag aaaatacttt caaatattcg acgagctgta
      481 acaaacgtta actgtaataa ttaggatttt aacactaaga gtgttacctt agctatgcgc
      541 attagggaaa gacccacagc gcagaaccct caaaaaccca agaaatgaaa acagcaaaaa
      601 gaacaacgct cagacttcac gtgaacggcg agcttggaaa cgacactatc ttaatttatg
      661 tctacagagc aaaagaagaa gccccgcact actacctaaa caggacatgg aacatcggaa
      721 ccagcaacca cccatactac gtcatgtgct atgaggtcga ggttatagaa gagtcaatta
      781 tttaaagaaa cgagaatggg aaccctaaaa aactacataa aagaggtcac aggggtcgag
      841 cttaccagca aacagctagg taggctccac gccctggccc tgaaagcctc agcggacgaa
      901 aaaaagactg tccaagaaaa gtacggaata gatgaagact ttgcccttgt ggttatcgag
      961 tggctggaat ataaaaagga gcgcagggaa agctacaaat cgccacgaag catcaaggcg
     1021 tttattacca ggctccagaa cttgtcagga aatgacctgg aaaaagctaa acgaattata
     1081 caaatatcaa tcgccaacaa ctgggctggc atttttgaac taaaggaa
//
LOCUS       DASRMS010000129        17214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_234166, whole genome shotgun sequence.
ACCESSION   DASRMS010000129 DASRMS010000000
VERSION     DASRMS010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 17214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17214
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_234166"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFC74_05880"
     CDS             complement(<1..554)
                     /locus_tag="VFC74_05880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK24898.1"
                     /translation="MAVRKENLESTYQLYRQSAEHDDLLNLVRAGERLVRHFSRIYTG
                     GMAEDALQAGREGLLKAVRRFEPEKGVAFATYASHCIMGEIRHYLRKEASYYRPGSIK
                     DLQNRVDRFVESVIKDRGQPPDLEEIAETLNVKVDGVVQAMRAGLVSLDEVEVKKIHS
                     LRYESFRLPIEDKLVLQQALERLS"
     gene            complement(563..1768)
                     /locus_tag="VFC74_05885"
     CDS             complement(563..1768)
                     /locus_tag="VFC74_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HZK24899.1"
                     /translation="MPLYAYQVLDRRGAVASGKLEAENEFSAAARLKKLGYTPLEVNE
                     AKTSAFKQMFQFKKKVTTGDLAMFSRQLASMLNAGIPLTRCLYALGEQAANQTLGETL
                     LDVANNVEGGMSFSASLRAYPGVFNDIYVDLISAGELGGTLETVLERLAQQLESEKLL
                     KDNVRSAMLYPSVVLCFAVLVMIAMLLFIVPVFLGFYPPDADIPALTSLIIGVSNSMR
                     GYWYLYLVFFAVLMLGVRTYVLSEAGKKTYDRLKFRIPIFGSLIQKTVIARFARTLST
                     LLAGGIPVLQALDSAGPAAGSSLIEEAVRVAGERIQEGQSIAAPLKESGLFPPMVTLM
                     ISVGEETGDLPGLLVRVSDFYEAEVATMSKGLTSMIEPLMIIGVGGLIGFMVIALYLP
                     MFSVITQIG"
     gene            1973..2335
                     /locus_tag="VFC74_05890"
     CDS             1973..2335
                     /locus_tag="VFC74_05890"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZK24900.1"
                     /translation="MHKLIKRAKNQKGFTLVELMVVVIIIGILVAIAVPVYNGVQANA
                     RKNAHNANVRTLEGAAALYISENGVDGKIKLTNDDLVPKYLQAWPTNPMKNGEAYKIG
                     LGTDGKITVSPARETVED"
     gene            2651..2911
                     /locus_tag="VFC74_05895"
     CDS             2651..2911
                     /locus_tag="VFC74_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK24901.1"
                     /translation="MDAIRECCAGLDVHQATVVACILKGPLDKKPKSEVREFPTVLSG
                     LLKLMDWLEEQGCLEVAMESTGVYRKPVYNVLEASYPEILPQ"
     gene            3628..4518
                     /locus_tag="VFC74_05900"
     CDS             3628..4518
                     /locus_tag="VFC74_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZK24902.1"
                     /translation="MTVNTENIVVSFIQHLQADGKGPKTILSYSGDVRAFLQWLEDKG
                     QSFHGHFSRFHITRYLNQLQELNYTPNTINKKVNSLHCFNHFLINQKLMQEKVVFPNK
                     DKIKIARGSEAEVAVFSKPELERLLFYIEDQAQVSQRDKVVVLLLLYTGLRVSELVNI
                     RLKDLDLLALNLKVVGKGGKYREIPLKSEVAEAVQIYLEGDRKAHKHATSPYLLLTQR
                     AGRMDKDTVNKLLRKIGSRIDLDIHPHKFRHTFCSRLVQLNVPLTTVSKLAGHASIQT
                     TAHFYINTSRQDKEQAVSLL"
     gene            4643..5044
                     /locus_tag="VFC74_05905"
     CDS             4643..5044
                     /locus_tag="VFC74_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24903.1"
                     /translation="MRLIYTWQDLVLLRQAKALPEDYLKYLEEMFLEVKDLLEYGKPK
                     ESFSLHNHGGIIVLERGDNAKDLSAVGLNPQDGGLLGTAPETTNKVVLSSIVLYETLV
                     VHNNEYACFYIVPENLDDEVETWLRWQAGTD"
     gene            5114..5569
                     /locus_tag="VFC74_05910"
     CDS             5114..5569
                     /locus_tag="VFC74_05910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003427593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HZK24904.1"
                     /translation="MTKKPRKRIDIVSLKLVRESSILYPLRQVSSPKDAADLLQQFVG
                     DCDREKFTVLCLDTKNQPISLSVISVGSLNSTLVHPREVFKTALLCNSASIILAHNHP
                     SGVPEASREDVDVTKRLCEAGKILGIEVLDHIILGAEKRFCSIKEAGKM"
     gene            5680..5832
                     /locus_tag="VFC74_05915"
     CDS             5680..5832
                     /locus_tag="VFC74_05915"
                     /inference="COORDINATES: protein motif:HMM:NF045139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24905.1"
                     /translation="MIVKSAAIGAVLGSVFGLWANGLSGVFPGAVIGAAAGIALRKWI
                     FRTFWH"
     gene            5869..6012
                     /locus_tag="VFC74_05920"
     CDS             5869..6012
                     /locus_tag="VFC74_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003372914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24906.1"
                     /translation="MNVTILILRIILLILGGSTAACAVARVSGESGISFSTLWEQLPD
                     RYK"
     gene            6422..7402
                     /locus_tag="VFC74_05925"
     CDS             6422..7402
                     /locus_tag="VFC74_05925"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK24907.1"
                     /translation="MGNDFYNRVKAALDSNNLKPYHKNTLRVVIHHLDGMSSPEIAKK
                     LDITLRTVQRHLKKYHELGEDIIQELCAKGNKHKLTPEQKAKLDTNIKEGAYQSGYMV
                     NTWTARILAQHIKKQFDVVLNIESCRQLLQKIDNPSDDNYEKSDIPPHDNTWAFLEIF
                     LGYYRSTGKYHIGKAKCYPSDFLKSEDEILKENIDLNTPVAKWYLLLAENKQTGERRH
                     EIVLSNTTNRLNHNRGFLRKIIKGTRPNDVLLLLTNNALNRTLVRRMRYKNRAREIRV
                     KFFRLNNDKSLDQFREDLLRDFDLLNKENQRKKIKETTIHRIEQYLTQQR"
     gene            7646..8068
                     /locus_tag="VFC74_05930"
     CDS             7646..8068
                     /locus_tag="VFC74_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24908.1"
                     /translation="MKTKKKKKKMVDILTAFTPSEAEYQAKSRAGALSVVNTEQHGKR
                     VKISKHLLQQIGNPTSVQFSFNKNKIAVAANLRGCTVFYDVKKGGCIYSSALVQEITQ
                     KFELDFSNITCISTGQAKYQTLDDGETVAIIKMKKKKN"
     gene            8114..9541
                     /locus_tag="VFC74_05935"
     CDS             8114..9541
                     /locus_tag="VFC74_05935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage/plasmid primase, P4 family"
                     /protein_id="HZK24909.1"
                     /translation="MSKKHSNIPTEAKDIESLKAVGFTFNDNEQISGINGNVFARYIL
                     TLYTLLFSPGSRFYQYRKGRWEYVENIVLSKRLRKKLHEFVPDFWTTVVEKTYIEALK
                     REAPYVTKLDADRDSLNLSNGMLNLDSFKLTKHRAKYHSSIQIPIIYDKILDCPHFKN
                     FLADVFEEDAERIAVVQEMLGYCLTSDTSAQKCFFLFGKGANGKSILAEIMLNLCGKE
                     NVSAIPIKDLSKPFSRYELVDKLLNLATENEVSEKGLNTTHFKAIVAGDPIQVEKKHE
                     QSFTYQPVCKLVFSLNNLPYTKDRSLGFQRRLIVIPFNKTFREDDPMTKNYNELLDSL
                     LAELSGILNFALEGLKRLRKNKYRFSHSTAIDNALKKYRELINPHLLFVSDAVEQGDQ
                     HDRLYNDDLVRTFKTWCENNGYLELRKRSNRAILADIRDVLLNERFDFDHGDHCKSGK
                     KGRYTHKVKLKVQSSRTGLMLSRKR"
     gene            9691..10323
                     /locus_tag="VFC74_05940"
     CDS             9691..10323
                     /locus_tag="VFC74_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24910.1"
                     /translation="MKSPTAIFATNSRPGGTESIFCVAEQKITVTNRHTGTLNPKCSL
                     FSIAFLWHRLQKRKCRAVSTQTLVGPLRYQEGKKMYLNTRKRKPRISEGEHDAVIGLP
                     KTQKLIKQNKMWYRVTIPFIIKRSRFDDTIVVNFYATIDSTISDNLILLFENILGNIR
                     PTLYNLDKLEGRAVKALVVHRIDKKGNTWENVIAARKNVIRRNRHNSRFK"
     gene            10384..11244
                     /gene="xerA"
                     /locus_tag="VFC74_05945"
     CDS             10384..11244
                     /gene="xerA"
                     /locus_tag="VFC74_05945"
                     /inference="COORDINATES: protein motif:HMM:NF040815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008907 - integrase activity [Evidence
                     IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase/integron
                     integrase"
                     /protein_id="HZK24911.1"
                     /translation="MLFLDAIELLIKNMETIGRSSATIKLYRRDLRRFAHHLACKQNG
                     PVYLEDITAADLEEFLHNLKELGIAPNSRSRYLYTFRAFYKFAYKKELVDRDISQSIE
                     LIKIPAKERQYLSQEEVEELANAIRQNIIRLVVLFLANTGLRISECLSLTLDDVDLDK
                     SMIRVIQGKGGKDRNVPINQTLLPLLKDYRNNWRDAYGSDLFFATKKSGSLSNSYINT
                     VIRNTVKKLGWKKQVSCHTLRHSFASSLVKKKVGLVEIQKLLGHSSLKVTSVYTHVDM
                     DQLNEAVNAL"
     gene            11257..12045
                     /locus_tag="VFC74_05950"
     CDS             11257..12045
                     /locus_tag="VFC74_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24912.1"
                     /translation="MAVNAIDKAPIYEKQVQTILDGLAAGRTKEELAEQLGYTNVKSL
                     DNYMRRKNFSWDGRRGIFFPTVKKLAAKKLQLPKQDYSKSGVVVDLFAGGLDSKEIAQ
                     KLGFPSHKDLAQYMTDKDYIWDAEHCNYVKTNVKQLKQKQQSPEPIPVADAAAANSES
                     QNSDDLTELLSFLPLLRQLQARKERLGELLESIPLDSAQLPHYAIPGTPVTKSVYMVN
                     TIDKLVKEYSEERNVSQRQIFELALLEFFKKYGYRAEVDRLLLN"
     gene            12258..12554
                     /locus_tag="VFC74_05955"
     CDS             12258..12554
                     /locus_tag="VFC74_05955"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZK24913.1"
                     /translation="MRLGFTLVELMVVVVIIGVLVAIAVPVYNSITGDAADKADQATL
                     RTVNGAVMMYDAANGEKSFDGTIADLVGDFLQETPTLNSGTLTWVPADHKFIIN"
     gene            complement(13056..13217)
                     /locus_tag="VFC74_05960"
     CDS             complement(13056..13217)
                     /locus_tag="VFC74_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24914.1"
                     /translation="MWVADDPTNNVATAVTMGDLVTDFIDETPKCPTDGDDYTFTNGK
                     AVCPNGHTY"
     gene            complement(13375..13938)
                     /locus_tag="VFC74_05965"
     CDS             complement(13375..13938)
                     /locus_tag="VFC74_05965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24915.1"
                     /translation="MNIKAHKLAPIYERQVTYILEALVQGQSRKELAQKLGYTNAKSL
                     DNYMRRKNFSWSRQYGTFIPTISKTAETRKEVGSNSAIAATALALFSQQYEAKEVATQ
                     LGFQDHRELYDFMNQEEYQWDAKHANYHQRAEGSSCNLPHPEPAPVPVADKAGTDLAE
                     LISYLPLLRKLQKREQLTELLEAASSS"
     gene            complement(13954..14814)
                     /locus_tag="VFC74_05970"
     CDS             complement(13954..14814)
                     /locus_tag="VFC74_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HZK24916.1"
                     /translation="MLFNEALKLFQDHLLTIGRSPNTIHRYLTDLTTFYHAQEHKQNG
                     AAYLADINSTDLEQFLYNLQQQGIAPNSRSRFIYTFRSFYKFAYKKELVPRDIAQTLD
                     VIKLPTTERTYLNQCELEELIAAIQHKLIRLLVIFLANTGLRISESLHLTLHDVDLAA
                     GLIQVRQGKGGKDRKVPINQKLLPLLQDYRENWRDAYGSDFFFATKKTGSLSNVYVNS
                     IIRQTVKKLGWSKQVSAHTLRHSFASNLIRKKVGLVEIQKLLGHSSLTVTSIYTHTDL
                     AQLTAAVNTL"
     gene            complement(14999..15484)
                     /locus_tag="VFC74_05975"
     CDS             complement(14999..15484)
                     /locus_tag="VFC74_05975"
                     /inference="COORDINATES: protein motif:HMM:NF022986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3231 family protein"
                     /protein_id="HZK24917.1"
                     /translation="MNIVIFDYAIIPLLYSVFLYFSKPFINFILRIQVYFNYAHDTDF
                     KLMIKNIGLSMLEAQAAELEKQCELYGIPMPSRPPKNVTLHGDSIIFSDEYMFRQIFE
                     GCQHFLQHIARCISTAVHNDALREIFYKFFKDEMTIFNNICKYGKTKGYLHVAPTYKA
                     T"
     gene            15492..16811
                     /locus_tag="VFC74_05980"
     CDS             15492..16811
                     /locus_tag="VFC74_05980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HZK24918.1"
                     /translation="MPMKYRLIQINDNLVTHSGLSIVGQMLAKTSLKKRLDRSAIPKI
                     TDPTILHSEVVKSYVGLLCQGKSDFDHIEAFREDIFFPQALRVKKVPSSPTLRQRFNL
                     AGKRWKEIILEESAVMLKALDAVLTPCIRDFVPLDIDVTPFDNSNTKKEGVNRTYKGH
                     DGYAPIFAYLGQEGYGVNVQLREGKDHSQNGTPEFLTQTIQYARMITDVPLLIRMDSG
                     FDSVDNLKVCLNTETPTDFIIKRNLRRESKEGWLNIAEQNGICCLERDGKKVYLGDLV
                     TKVKGVDLPVRMVYRVVERTITASGQYLMMPDLEVETYWTTLPDSPATIIDLYHNHGT
                     SEQFHSEIKGELDLERLPSGKFDTNHIVLTMGLLAYNILRFIGQASLGAKAPLRKKVQ
                     RRRIRTVIQNLITIAAKLISHARSWTLGFGRHSPWFQTFQHIYRIVS"
     gene            complement(16974..17144)
                     /locus_tag="VFC74_05985"
     CDS             complement(16974..17144)
                     /locus_tag="VFC74_05985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24919.1"
                     /translation="MVQIGNFHFGKADSAEKLNLSLQEAALVWDVLVSRYKCIEETNI
                     YPESVKPLLKPV"
BASE COUNT         5393 a         3905 c         3529 g         4387 t
ORIGIN      
        1 tcactcaacc gctctaatgc ctgctgcaaa accagcttgt cctcaatcgg caacctaaat
       61 gattcataac gcagagagtg aatctttttt acctctacct catccaggga aactaaaccc
      121 gcccgcattg cctgtacaac accatctacc ttcacattta atgtctcagc aatttcctct
      181 aaatcaggtg gctgtcctct atctttaatc acactttcca caaaccgatc tacccggttc
      241 tgcaaatctt taatcgaccc cggtcggtaa taggaagcct ctttccttaa gtaatgacga
      301 atctccccca taatacaatg agaagcatag gtagcaaacg ctaccccttt ctccggttca
      361 aaacgacgca ccgccttaag taagccttcc ctacctgcct gcagagcatc ttccgccatc
      421 ccccccgtat aaatacggga aaaatgacga actaagcgct ccccagcccg caccaaattc
      481 agtaaatcgt catgttctgc tgattgacga tacaactgat aggtactctc taaattttcc
      541 tttcgcactg ccatattact acctacccta tctgcgtaat cacagagaac atcggtaaat
      601 acagtgcaat taccataaaa cctataaggc cgccaacacc aataatcatt aatggttcaa
      661 tcatactagt caggcctttt gacatcgtag caacttccgc ttcataaaaa tcagacactc
      721 ttaccaatag ccccggtaaa tcccccgttt cttctcccac tgagatcatt agtgtaacca
      781 tagggggaaa caaaccactt tcttttaatg gtgctgcaat actctgtccc tcctgaatac
      841 gctcacctgc cacccgtaca gcctcctcaa tcaggctgct gcctgctgcc ggcccagccg
      901 aatccaaagc ctgtaaaacg ggaatacctc ccgccaacaa ggttgacaaa gtccgggcaa
      961 agcgagcaat cactgtcttt tgtatcaaac taccaaagat aggaatacga aattttaacc
     1021 tgtcataggt cttcttgcct gcctcactca acacataggt tcgcacacct aacatcaaga
     1081 cagcaaaaaa taccaaatat aaataccaat agccacgcat ggaattactg acaccaataa
     1141 taagacttgt caaagcggga atatctgcat ccggcggata aaaccccaaa aagaccggaa
     1201 caatgaaaag caacatggct atcatcacca acactgcaaa gcacaacacc acagagggat
     1261 aaagcatcgc cgaacgaaca ttatccttta gtaacttttc gctctccaac tgctgcgcca
     1321 agcgctccaa aacagtctcc agcgttcctc ccaattcacc agcactaatt aaatcaacat
     1381 aaatatcatt aaatactcct ggataagctc tgagggatgc cgaaaaactc atgccacctt
     1441 ccacattatt agccacgtcc aacaaggtct cacccaaagt ctgattagca gcctgctcac
     1501 ccaaggcata caaacaacgc gtcagtggaa tccctgcatt aagcatagaa gccaactgcc
     1561 ggctaaacat tgctaaatca ccagttgtaa ccttcttctt aaattgaaac atctgcttaa
     1621 aagctgaagt tttcgcttca ttaacctcta acggcgtgta acctaatttc ttcagtctcg
     1681 ctgccgcaga aaactcattc tccgcctcca gcttcccact agcaaccgcc ccccgcctgt
     1741 ccaacacttg atatgcatac agtggcatac ataacccccc atgcttattt accaaaccct
     1801 tctacaatct ccccatacat cttttaccaa gaatacttct cgacaatcag caaatctcct
     1861 tcacatattc aagtattata aaaatatttt tgcaaaactt gtctaaatat ctcctctcat
     1921 ggtataacat aggcagaaca caccccacaa ggggaaacaa ggaggaaata atatgcataa
     1981 gttaataaaa agagctaaga accaaaaagg cttcacactg gttgaactga tggtggtagt
     2041 tatcattatc ggtatcttgg tggcaattgc tgtgccggtt tataatggtg tgcaggcaaa
     2101 cgccagaaaa aatgctcata atgccaatgt gagaactcta gaaggtgcag ctgcgctgta
     2161 tatttcagaa aatggtgttg atggtaaaat taaattgaca aacgatgatt tagtgccgaa
     2221 gtacttacaa gcatggccaa ctaatccaat gaaaaatggg gaggcttata aaataggctt
     2281 aggcactgac ggcaaaataa ctgtatcgcc agctcgtgaa actgttgagg attagtatac
     2341 agacaagcca gcccttacat aaaacgggct acccggaaat tcttccgcaa taagctccat
     2401 aatttcccaa aacaaacatg cctttaacag gcacaatcta gtatgaaaat ggtaacgtag
     2461 taaatattta gcatcatgcc aatactatag atatgcagga caggaaggtc tcccccttat
     2521 tggatgcaag actccattcc taaaactgac tacaactgct ttgagaacaa catattgttg
     2581 ctttgagcac atttatcaaa tttcaaatta tcaggcggtt gtgccctgca gacaaggagg
     2641 atgctaaaag ttggatgcaa tacgagaatg ctgtgcaggt ttagatgtac atcaagctac
     2701 tgttgttgct tgtattttga agggtccgct agataagaaa cctaaaagcg aagtacgaga
     2761 atttcctact gtgttatcgg gtttactaaa attaatggat tggcttgaag aacaaggatg
     2821 tttagaagtc gccatggaaa gtacaggcgt ttatcggaag cctgtatata atgtgctgga
     2881 agcatcatac ccggaaattc ttccgcaata agctccagaa tttcccaaaa ggggtcagtc
     2941 cctcattata gtaaagcatt aaagcagaag ccagttctct catgagggct ggctttcttc
     3001 aatccctaat ctacaacagc agcataccgc tcgctgaaaa acatcaccca gaatcactac
     3061 ccggaaattc tttcgcaata agctccataa tttcccaaaa agggtcagtc cctcaccata
     3121 gtaaagcgct aaagtaaacg gcaaattcca gggactaccc tgcaactgtt cccgtaaagc
     3181 aacctaatca taaataaatc tcgcctaatc tgtctatcac ccaaaagggt gaaaccatcc
     3241 ccctccagaa tctttttaat tgttctccta cagcttttta gcaaataagg taaggacgag
     3301 cagaatccaa atcatccacc gaaagccagc tctctcttga gggctggctt tcttcatgtc
     3361 ctaatctcta gctacatacc atcactgtct ggaaaacatc actcataatc actgtaaaac
     3421 attctgccat cacttcaacc gaatataaac cgatgcccag catcattttt tccagattat
     3481 tccccatcaa atctcgctga agaattagta ttctgcgacg agtacccgcc cctagaggac
     3541 accaaatcct ctgggggctt ttttgcattt caccatgttg ctagcaagcg agtcctaaga
     3601 ccatctctct agaaaggagc tgcacacatg accgtaaaca ccgaaaacat cgtcgtttct
     3661 ttcatccagc acctgcaagc agacggcaaa ggtccgaaga ccatcctctc ctacagcggt
     3721 gacgtcaggg ccttcctgca gtggctagag gacaaaggac agtccttcca tggccacttc
     3781 agccgcttcc acatcacccg ctacctcaac cagcttcaag agctgaacta cacccctaac
     3841 accatcaaca agaaagtcaa cagtctgcac tgcttcaatc acttcctcat caaccaaaag
     3901 ctgatgcaag aaaaggttgt cttccccaat aaggacaaaa tcaagattgc cagaggtagc
     3961 gaggcagaag tcgctgtgtt cagtaaacca gagttggaaa gattgctttt ctacatcgaa
     4021 gaccaggcac aagtcagcca gcgagacaaa gttgttgtac ttctgctcct gtacaccgga
     4081 cttcgggtca gcgaactagt caacatcaga ctgaaagatt tggacctgct ggcactgaac
     4141 ctgaaagtcg ttggcaaagg cggcaagtat cgagaaatcc ctctcaagtc ggaagtggca
     4201 gaagctgtac aaatctacct tgaaggtgac agaaaggctc acaaacacgc caccagcccc
     4261 tacctcctcc tgacacagcg ggcaggaagg atggacaaag acaccgtcaa caagctgctg
     4321 cgcaagattg gcagcagaat cgatctagac atccaccctc acaagttcag acacaccttc
     4381 tgctctaggt tggtccagct gaacgtccct ctaaccactg tctccaagct tgccggacac
     4441 gcctccatcc agacaacagc ccacttctac atcaacacca gccgccagga caaggagcaa
     4501 gctgtctccc tcctgtaaaa gaccggggca aacggggcgt agtgtctgcc ccagaagccc
     4561 cagaagctgc acaggggaca gggacctgga aagttgtgcc acatacatca tctctgtctg
     4621 acaaacaaaa ggagttgaaa aaatgcgtct catctacact tggcaggact tagtcctgct
     4681 acgtcaagca aaagctctgc cagaagacta cttaaagtat cttgaggaaa tgttcctaga
     4741 ggtaaaagac ctactagaat acggcaaacc aaaagaaagc ttttccctac acaaccatgg
     4801 aggaatcatc gtccttgaaa gaggtgacaa tgcgaaggac ttgtcagctg ttggcctgaa
     4861 tccacaagac ggagggctcc taggtacagc tccagaaacc acaaacaaag tggtgctgtc
     4921 gagcattgtc ctgtacgaaa cgcttgtagt gcacaacaat gaatacgcct gcttctacat
     4981 cgttccagag aacctggatg atgaagttga aacctggttg cgttggcaag caggaactga
     5041 ctaggcccga atctccacag cggaggttcg ggtctttctt tgtgtataga aaactagaag
     5101 ggagcaaaca aacatgacca aaaaacctag aaaacggatt gacattgtca gcctcaaact
     5161 tgtcagggaa agcagcatcc tctacccctt gcggcaagta agctctccta aagatgctgc
     5221 tgacctacta caacaattcg ttggcgactg tgatagagag aagttcactg tactctgcct
     5281 ggatactaaa aaccaaccca tcagtctcag tgtaatctct gttggcagtc ttaacagtac
     5341 tctcgtacac ccgagagaag ttttcaaaac tgctttgttg tgtaacagtg cctccatcat
     5401 cctagcccat aatcacccat caggagtacc agaggctagc cgtgaagatg tggatgtaac
     5461 caagagactc tgtgaagcag gaaagatcct gggaatagaa gtgcttgacc acatcatcct
     5521 gggggcagaa aaacgtttct gcagcattaa agaagctggc aagatgtaac tgaaacaacg
     5581 cacctaataa agctatatta tccctttagt gatgcttaat cgataggtaa tcaccctagg
     5641 aacataggaa caaagaaact acgaaaggag actgatagca tgattgtaaa atcagcagca
     5701 atcggagcag tactaggcag cgtcttcggt ctgtgggcaa atggactcag tggagtcttt
     5761 cctggtgcag tcataggagc agccgctgga attgccctgc gcaaatggat tttccgtacc
     5821 ttctggcatt agcaaaaagc aacaaaaact cagggaaagg gaaacaacat gaacgttacc
     5881 atcttaatcc tgcgcatcat ccttctgatt cttggtggca gtacagcggc ttgtgctgta
     5941 gccagggtca gcggagaaag cggaatctcc ttcagtacac tgtgggaaca attacctgac
     6001 aggtacaagt aggcaagaaa tcaggaaggg agcaaaatcc cttctttttt tttgcttggt
     6061 ccgtccaccg cttcccacta gggagagcgg tggacggtgt ggggcgactc cgggtcacaa
     6121 aaaaagattg cgacacagca aaaaacgttg ttctgtcagg attaaaccac cgttgtggcg
     6181 aggtggcgct agaaatcagc catctgtttg ttcaggaccg aggtacaaag gtctgcagtg
     6241 aaaactagcg acacaaccgc caccaacacc caagaacctg gactcttcca cattttcggc
     6301 gtggcgacaa gagggtgtaa gcgacaaaga accggcactg tccgcttgta aaacagcgtg
     6361 tacaaacaag cttggaaggt gtactatatc aatagtagaa aaactgatga ggtactctag
     6421 catgggtaat gatttttata atcgggtaaa agctgctctg gatagcaaca accttaaacc
     6481 gtaccacaag aatacattac gagttgttat acaccacctt gacggaatga gcagtcctga
     6541 aattgccaaa aaacttgata taactctacg aaccgtacag cggcacctaa aaaaatacca
     6601 tgaattaggt gaagacatta tacaagaact ttgtgctaag ggcaataagc acaaattaac
     6661 gcctgaacaa aaagcaaaac tagatactaa tattaaagaa ggtgcctacc aatctgggta
     6721 tatggtgaac acttggacag ccagaattct tgcacaacac attaaaaagc aatttgatgt
     6781 cgttcttaac attgaatcct gtcgtcaatt attgcaaaag attgataatc cttccgacga
     6841 taattatgag aagagcgaca ttccacccca tgacaatact tgggcatttc ttgaaatctt
     6901 cttagggtat taccgctcca ctggtaaata tcatattggt aaagcaaaat gctacccttc
     6961 agacttcctt aaaagcgaag atgaaattct taaagaaaat atagacctaa atacccctgt
     7021 tgcaaaatgg tacttactgc ttgctgaaaa caaacaaacc ggagagcgtc gtcacgagat
     7081 agttctttca aacacaacaa atagactcaa tcataatcga ggttttcttc gtaagatcat
     7141 taaaggaact cgccccaacg atgtgttgtt gttgctgacg aacaacgcac taaacagaac
     7201 tcttgttcgg agaatgaggt acaaaaacag agctagagaa atacgagtta aattctttcg
     7261 attaaataat gataagtctt tagaccaatt ccgtgaagat ttattaagag attttgattt
     7321 actcaacaaa gaaaaccagc gaaaaaaaat caaagaaaca acaattcata ggattgaaca
     7381 gtacctaacg caacagcgtt aggttttttt tatgctaaaa acgacgcatt taattggatt
     7441 gtttaagttt cctaaaatac ggcgaaagat gaacaaaaac gatggcagct caatataatc
     7501 aaaatcgcat aagtaaaatt ttctgctctc tgggcgaagt aaggcgcatc tctcttttgg
     7561 taatttttaa catcctgttt gtttttttcc tgctgcaccc gttacaatta gtatagaaga
     7621 aattcaaaag aaaggaatgg ttactatgaa aacaaagaag aaaaagaaaa aaatggttga
     7681 tattctaacg gcgtttacac catctgaagc agagtaccaa gcaaaaagca gggctggtgc
     7741 actatccgtt gtcaacacgg aacagcacgg gaagcgtgta aaaatctcaa agcacctgct
     7801 gcaacaaatc ggaaatccca cttctgtgca attcagcttc aacaaaaaca agattgcagt
     7861 cgccgcaaac ttgcgggggt gcacagtgtt ttacgatgtg aaaaaaggtg gctgcattta
     7921 ttcatctgcg cttgtacaag agataacgca aaagttcgaa ctagattttt ctaacatcac
     7981 ctgcatctcc actggacagg caaaatatca aaccttggat gacggtgaaa cagtggcaat
     8041 catcaaaatg aagaagaaga agaattaagt gggaggaagc atgcttcctc ccaccctgaa
     8101 agggggaaat tctgtgtcaa aaaagcacag caacatacca actgaagcaa aagatatcga
     8161 gtcacttaaa gcagttggct tcacctttaa cgataatgaa cagatttcag gtattaacgg
     8221 taatgtattc gcgagataca ttctaacact ctacaccctt ctgtttagcc ctggctctcg
     8281 cttttatcaa tatcgcaagg gtcggtggga atatgttgaa aatatagttc tgtcaaagcg
     8341 tttacgtaag aagcttcatg aattcgtccc tgatttctgg actactgtgg ttgaaaaaac
     8401 ctacattgaa gcgttgaaac gagaggctcc ttacgtcaca aagttagacg cagaccgaga
     8461 ttctctaaac ttatcaaacg gaatgctcaa cctcgacagc ttcaagctga caaaacaccg
     8521 ggcaaaatat catagcagta tccagattcc aatcatctat gacaagatat tagattgtcc
     8581 tcactttaaa aattttcttg ctgatgtctt tgaggaagac gcagaacgca tcgctgtggt
     8641 tcaggaaatg ttaggctact gcctgacatc agatacttct gcacaaaagt gtttcttcct
     8701 attcggcaaa ggggccaatg gtaaaagcat actggctgag ataatgttaa acctgtgcgg
     8761 caaagagaat gtatcagcga taccgattaa agacttgagt aaacccttta gccgttacga
     8821 actggtcgat aaactactca atctggcaac ggaaaacgaa gtgtcagaaa aagggttgaa
     8881 cacaacccac tttaaagcca tcgttgcggg cgaccccata caggtcgaga aaaagcacga
     8941 gcagagcttt acctatcagc ccgtctgcaa gcttgttttt tcactaaaca atcttccgta
     9001 cacaaaagat cgcagtttag gatttcaaag aagactcatt gttataccct tcaacaaaac
     9061 cttccgtgaa gatgatccta tgactaaaaa ctacaatgag cttttagaca gcttgcttgc
     9121 cgaactaagc ggcattctga actttgcctt agaaggttta aagcgtctgc ggaaaaacaa
     9181 atatcgcttt agtcattcaa ccgctatcga caatgcgctg aagaaatacc gggagttaat
     9241 caaccctcac ctactctttg tcagtgatgc ggttgagcag ggagaccaac atgaccgact
     9301 ttacaatgac gacttggttc gtacattcaa gacttggtgc gagaacaatg gttacctaga
     9361 actgcggaaa aggtcaaacc gagcaattct tgcagatata cgagatgttt tactgaatga
     9421 gagatttgat tttgaccatg gtgaccactg caaaagcggg aaaaaaggac gctataccca
     9481 taaggtaaag ttaaaggttc agagttcccg tacaggctta atgctatcta gaaagagata
     9541 gcattaagcc caagaaaggg aaagcacaga atcagaagtt tatctaatag caatgaaaaa
     9601 ggagctggga aactggcgga ggcttgacaa attcacatgt ttcgtgtttg tcatagaggg
     9661 cggtgagtcg ttcaaactga tctcaatcga atgaaaagtc cgaccgccat cttcgccaca
     9721 aactcccggc caggaggaac tgaaagcatt ttctgcgtag cagaacaaaa aatcaccgtc
     9781 acaaaccgcc acacaggaac gctgaaccca aaatgcagtc tgttctcaat agcttttctc
     9841 tggcacaggc tgcaaaaaag aaaatgtcgg gcagtctcca cacaaacact tgttggaccg
     9901 ctcagatatc aggaggggaa aaaaatgtac ttaaatacga gaaagcgcaa accacgaatt
     9961 tcagaagggg aacacgatgc agtaattgga ttacccaaaa cacagaaact aatcaaacag
    10021 aacaaaatgt ggtatagggt aacgatacct ttcatcatca aaagatcaag atttgacgat
    10081 acaattgttg taaacttcta cgcaactatc gactcaacca taagtgataa cttgattttg
    10141 ctctttgaaa acatccttgg caatatcaga ccaactcttt ataatctcga caaattagag
    10201 ggtagagccg tgaaagctct agttgtgcac agaatcgaca agaagggtaa cacatgggaa
    10261 aacgtgattg ccgcacgcaa aaatgtgata cgtagaaacc gacataattc ccgattcaaa
    10321 taaaaagata aacttatatt atggtaatgt tggaataata aagaaatatt aaaggaggaa
    10381 aacatgctgt tccttgatgc aatcgaactg ctaataaaaa acatggaaac cataggtcga
    10441 agttcagcaa ccattaaatt atatcgccga gatttgcgta gatttgccca ccaccttgct
    10501 tgtaaacaaa acggcccggt gtacttggaa gacataactg ctgccgacct agaagaattc
    10561 ctgcataatc taaaagagct gggcatcgca ccaaacagcc gcagtcgcta cctttacacc
    10621 ttccgggcgt tttacaagtt cgcttacaag aaagaactag tggacagaga catctctcag
    10681 tcgattgaac ttatcaaaat accagcaaaa gagcgacagt atctatcaca ggaagaagtt
    10741 gaagaactgg ctaatgcaat ccggcaaaac atcatcaggc ttgttgtgtt gtttcttgcc
    10801 aacacaggtc ttcgcatctc tgagtgccta tccctgacac ttgacgatgt agaccttgac
    10861 aaaagtatga ttcgagtcat ccaaggtaaa ggcggcaagg acagaaatgt tcccatcaac
    10921 cagacgctgc ttccattgct gaaagactac agaaacaatt ggcgtgatgc ttacgggtcc
    10981 gatctgttct ttgccactaa aaaatccgga agcttgtcta atagttacat taacactgta
    11041 atccgaaata ctgtaaagaa actgggttgg aaaaaacaag tctcctgcca tactctccgt
    11101 cactcttttg cctcaagtct tgttaagaaa aaagtcgggc tagtggaaat ccaaaagctt
    11161 cttggccata gcagccttaa agtgacatct gtctacaccc acgtagatat ggatcagcta
    11221 aacgaagctg taaacgcatt gtaaggaggt gatacattgg ctgtaaatgc aatcgacaaa
    11281 gcacccatct acgaaaaaca ggttcagacc attcttgatg gactggcagc aggcaggacc
    11341 aaggaagaat tggcagagca gttgggctac actaacgtca aatcactgga caactacatg
    11401 cgtcggaaga atttttcctg ggacggtcgc agaggcatct tcttccccac tgtaaagaaa
    11461 ttagctgcca aaaagctgca gctgcccaaa caggactact ctaagtctgg cgtagttgtt
    11521 gacctctttg ctggcgggtt agatagtaaa gagattgccc agaagttggg cttcccgagt
    11581 cacaaagact tggctcaata catgactgac aaagattaca tttgggatgc tgaacattgc
    11641 aactacgtga aaactaacgt taaacaactc aaacagaaac agcagagccc agaacccatt
    11701 cctgtggcag atgccgctgc ggccaacagc gagagtcaaa acagcgatga cttgacagaa
    11761 ctcctctcct tcctcccgct cctgcgacag ctgcaggcac gcaaagaacg tctgggagag
    11821 ctgcttgaat ccatccccct tgactctgcc cagctgccac actacgcaat acccggtaca
    11881 cctgtgacaa agtcagtcta tatggtcaat accatcgata agttggtaaa ggaatacagc
    11941 gaggagcgga atgtcagtca gcggcaaatc tttgagctag ccctactgga attcttcaaa
    12001 aaatacggct acagagcaga agttgaccga ctgttactaa actagcagta caggagcggg
    12061 ggacaaacat cccccgctcc tcctccatgc cctacatcaa cttaatggaa atctaatgga
    12121 aaccttttga tcatcgttcc attaggaaat ctgatgttaa attaatgatg aatcttctaa
    12181 tggctcgtca aaaaggtcca gaaggaacct tatcttacaa gatatcctac ttaatttgac
    12241 aaatttggga aattgtagtg cgtttaggct tcacactagt tgaactgatg gttgttgtag
    12301 taattattgg ggtgctggtg gcgattgctg ttccggttta caacagtata actggtgatg
    12361 cagctgataa agcggatcaa gcaactctaa ggactgtaaa cggtgcggta atgatgtatg
    12421 atgcagctaa tggggaaaaa tcctttgatg gaaccattgc tgatttagtt ggtgattttc
    12481 tccaggaaac acctactctt aacagcggca cattaacgtg ggtaccagcc gaccataaat
    12541 ttattatcaa ctaatcgtac ctcctaaccc agctatctga attaggtagt tcttcaattt
    12601 tctttagcac tccgaaggct acagactaaa gcataaacca gtcctttaca ggggctggtt
    12661 tttttgtcgt taagtaaact atatcaacct tttaaaaatg aattacctga taaagcccca
    12721 tgtggtaagg ttttattatg aagggtatag tacgaaaaat cttttctgat cactgggaag
    12781 gttttttaaa acagtcggta taactaaaat tagaccaaca attattcgcg aagttgagtg
    12841 aatgattagt tgtgctgact tgcccctgtg tcatcatgaa ggattttttc taaagacgtg
    12901 ctggattatt ttgatgttat taatccacca acaccactta gcggatctat caatttatat
    12961 gttacatgtg aaaaatgtgt gggtatcata tcacaaagaa aataagcacg ccctcaggcg
    13021 tgccttttat atttctagaa atctagttgt agagcttaat acgtatgacc gtttggacat
    13081 acagctttac cattggtaaa cgtataatcg tcaccatctg tggggcattt tggtgtttca
    13141 tcaataaaat cagttactag atcccccatt gttacagcag tagcaacatt gttggtaggg
    13201 tcgtctgcaa cccacatctg tatagctccg tcaatgattc ttaagtttga ttgacaggcg
    13261 cgttctttag cattatcagt cacgttatta taaactggaa tcgcaaccgc taccaaaaca
    13321 ccaataatca caacaactac catcagttca acgagtgtga agcctttttg attattagct
    13381 ggagctagca gcctccaaca attcagtcag ctgctctctc ttctgcagct tgcgcaggag
    13441 gggtaggtaa gagataagtt ctgctaagtc tgtgccagct ttatctgcaa caggcaccgg
    13501 cgctggctca gggtggggta aattacatga ggatccttct gctcgttgat ggtagttagc
    13561 atgttttgca tcccactgat attcttcctg attcataaaa tcatatagtt cccggtgatc
    13621 ttgaaaacct aactgggtgg caacttcctt ggcttcatat tgttgagaaa ataaggccag
    13681 agcggtagct gcaatggcac tattactacc gacttctttc cttgtctcag ccgttttgct
    13741 aatggtggga ataaaagttc cgtactggcg ggaccaggag aagtttttcc ggcgcatata
    13801 attatcaagg gatttggcgt tggtatagcc tagcttttgt gccagttctt ttctgctttg
    13861 tccctgtacc agggcttcaa gaatataggt aacctgtctt tcatagattg gtgctagttt
    13921 gtgagctttt atgttcataa ttgatacccc agactataaa gtattgactg ctgctgttaa
    13981 ctgggcgaga tcagtgtggg tgtaaataga agtgaccgtc aggctgctgt gaccaagtag
    14041 tttttgtatc tctaccagac ccaccttttt gcgaatcagg ttggaggcaa aactgtgcct
    14101 gagggtatgg gcggacactt gtttgctcca gcctaacttt ttaactgtct ggcgaatgat
    14161 actgtttaca tagacattag agaggctgcc tgtcttttta gtggcaaaga aaaaatcaga
    14221 gccataagca tcacgccaat tctctcgata gtcctgcagg aggggtagca gcttttgatt
    14281 aataggtacc tttctgtctt ttcctccttt gccttgccgg acctgaatga ggccggcagc
    14341 taaatctaca tcgtgtaatg ttaagtgcag gctttctgaa attctcaggc cggtgttggc
    14401 aaggaagata acgagcagac ggattaattt atgttggata gcagcaatca gctcttccag
    14461 ttcacattga ttaagataag tcctttctgt agtgggaagc ttgataacat caagagtttg
    14521 agctatatca cgtggtacta gttctttttt ataagcaaac ttatagaagg agcggaaagt
    14581 atagataaaa cggctgcggc tatttggtgc tatgccctgc tgttggaggt tataaagaaa
    14641 ttgctccagg tctgtactgt tgatgtctgc taaataggca gctccatttt gcttgtgttc
    14701 ctgagcatga taaaaggtag tcaggtcagt aaggtagcgg tggatagtat tgggactgcg
    14761 cccaatcgtc agaagatggt cctgaaatag cttcaaagcc tcattaaata acatctgagt
    14821 tacctcctaa atggttttat ctgctatcaa gcacctcctg cttcctttct atacaccgta
    14881 tcattactaa aatagatttg ccctctcctt cccatgcgaa ctgtgttttt tgtagcagac
    14941 ctaatctatg taaaaaaagc tgacaataaa taaggctacc atgcaaaaag ctacctgtct
    15001 aggtagcttt ataagtaggt gctacatgaa ggtagccctt ggtcttgccg tacttgcaga
    15061 tattattgaa aattgtcatc tcatctttga aaaacttata gaagatctcc cgcaaagcat
    15121 cattgtgcac ggcggtacta atacaacggg cgatatgctg cagaaaatgc tgacagccct
    15181 caaagatctg gcgaaacata tattcatcac taaagattat actatcacca tgcaaggtca
    15241 catttttcgg aggtctgctg ggcatgggaa taccgtatag ttcgcactgc ttctccagtt
    15301 cggcagcctg tgcttccagc atgcttaaac caatattttt tatcattaac ttgaaatctg
    15361 tgtcatgtgc ataattaaaa tagacctgaa tccgtaaaat aaaattgata aaaggcttag
    15421 aaaaataaag aaaaacactg tataataaag gtataatggc ataatcgaaa atgacaatat
    15481 tcaccctgga ggtgcctatg aaataccggc ttatacagat aaatgacaat ttggttactc
    15541 atagtggttt atctatcgtt ggacagatgc tagcaaaaac atcattaaaa aaacggttag
    15601 accgttcagc tattccaaaa atcacggatc ctacgatttt acacagtgaa gtcgtcaaat
    15661 cttatgtcgg cttactttgc cagggtaaaa gcgactttga ccatattgag gcttttcgtg
    15721 aggatatctt ttttcctcaa gccctacgcg taaagaaagt accatcaagc cctactttac
    15781 gtcaacgatt taatttggca ggtaaacgat ggaaagagat catccttgag gagtccgctg
    15841 taatgctcaa ggcattggac gccgttttaa cgccctgtat tcgggatttt gtaccccttg
    15901 atattgatgt aacacccttt gataattcaa acacaaaaaa agagggagta aaccgtacat
    15961 acaaagggca tgacggatat gcgccaattt ttgcttactt agggcaggag ggctatgggg
    16021 ttaacgtgca attacgagaa ggaaaagacc atagtcaaaa cggcacccca gaatttctga
    16081 ctcaaaccat ccaatatgct cggatgataa ccgatgtgcc gcttctcatc cgaatggatt
    16141 cagggtttga tagcgtggac aacctgaaag tatgcctgaa tacagaaaca cctaccgact
    16201 ttattattaa gcgtaatctc agacgggaaa gcaaagaagg ctggctgaac attgccgaac
    16261 aaaatggtat ctgctgcctg gaacgtgacg ggaaaaaggt ttacctcggt gaccttgtga
    16321 caaaggttaa gggggttgat ctccctgtac gcatggttta tcgtgttgtt gaaagaacaa
    16381 tcacagcatc aggccaatat ctaatgatgc cggatcttga ggtagaaaca tattggacaa
    16441 cacttccaga ttcgccagcg acaattattg acctttatca caaccatggc accagtgagc
    16501 aattccacag tgaaattaaa ggtgaactgg atttagagcg actgccctcc gggaaatttg
    16561 atacaaacca cattgtgtta acgatgggtc tgcttgcata caatatcctg cgttttatcg
    16621 gccaagccag tttaggtgcc aaagctccgt tacgtaaaaa agttcagcgc aggcggatta
    16681 gaacggtcat acaaaacctt attactattg ctgctaaact gatttctcat gcacgtagct
    16741 ggactctagg gtttggaagg catagtcctt ggtttcagac attccagcat atctatagga
    16801 ttgtaagcta gtaaaacaga gttagttctt ccataactcc tgactgacag gggctagttt
    16861 tctgtcccct aaaacagatt atgtctgtaa ctaaaattat taatgtctga agcgtgcaag
    16921 ctggctaatg aaattaagtt taattgggct catttaagag aatatttgcc gctttaaaca
    16981 ggttttaata aaggcttcac ggattcagga tagatattag tttcctcaat acatttataa
    17041 cggctaacga gaacgtccca gactaaggcc gcctcttgta acgaaaggtt taatttttcg
    17101 gcgctgtctg ccttaccaaa atggaaattt cctatttgca ccaaaacatg tccccccctt
    17161 ctggttagta ttagtagttt tcttctgaga gaggatcttc atacttatta ttta
//
LOCUS       DASRMS010000130         5342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_436235, whole genome shotgun sequence.
ACCESSION   DASRMS010000130 DASRMS010000000
VERSION     DASRMS010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5342
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436235"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VFC74_05990"
     CDS             <1..468
                     /locus_tag="VFC74_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HZK24920.1"
                     /translation="YIYSLLLAKEYLNSDLLLLHGDLVFDDAVLANTLASPQPNTVLV
                     NRQTALPEKDFKARLVNGEVKEISIKIFGDDCAFLIPLYKLSSTLIQAWLIEMEQFAA
                     RNELNVYAENALNNLLADYALSPVYFQQELCTEIDNLQDLAKVKAALAARGEQ"
     gene            471..1289
                     /locus_tag="VFC74_05995"
     CDS             471..1289
                     /locus_tag="VFC74_05995"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZK24921.1"
                     /translation="MTISDYRQLCQKNEAENYFFDKYIFRKVSIYFTIIFIKLRLTAN
                     QATFLSLLASLSSCYFLMQNTTAAMFLGPALIFTYYMLDHVDGELARYYIRQGKQQPS
                     LQGTYFDVLIHKYSTNLMLFFLGVSVYNFFNYRWAVLLGFAACIGMSAFPNLLASQVI
                     VQKIAQQKELVDNPAVVEVLNLLEKKQEQIKKVTSTNPLKRLKKIITELLFFPGALLM
                     IMLVTLADLIVPTFTLFNYPVNIRLLFLLAVTPIYLTNALRQTLKWFSKFAEIA"
     gene            1310..2119
                     /locus_tag="VFC74_06000"
     CDS             1310..2119
                     /locus_tag="VFC74_06000"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZK24922.1"
                     /translation="MNLQQYRQLCQKNDSENYLLDKLIFRKISIFITILLIKLKLTSN
                     QTTFLSLLASLASLYFLTLNTRAALLTAAALIFTYYILDHVDGELARYAIQTGQAKPN
                     LKGHYFDVLVHRYSSNLMLFCIGLSIYNHYGYRWAVLLGFMACIGLSSFPNVIAAQVI
                     AGKIATNRQLVHTDPALRKILAELETKEQQIAAVHSGSLLQKVKKLIIESLFFPGHII
                     VLIIVLIIDAYWPGLATLNLRCLLLVGFTLLYTLKTIIQSMLWLAKLKTIA"
     gene            2144..2833
                     /locus_tag="VFC74_06005"
     CDS             2144..2833
                     /locus_tag="VFC74_06005"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HZK24923.1"
                     /translation="MDKQLQEILSYFHQDELAYWLDSGTLLGLMREGQLLASDKDLDI
                     SIWDTELAQLQALLPSFRQAGYQIYAASYQGQIFKYNLTPSNLKKLRTVDINIYRSNN
                     NHAWCPMYYFKLSPTTEKKPPSKASPLKALRSLLRSTWKAINTKFTMNVKIDRLPWQP
                     FLIIGTWWIPQAFYDKRIFNEQYQVWIPAAWQDYLAFRYGNWQEPQTDWVFYRDDQGI
                     NPQSPQQLLNN"
     gene            2949..4292
                     /locus_tag="VFC74_06010"
     CDS             2949..4292
                     /locus_tag="VFC74_06010"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HZK24924.1"
                     /translation="MGTNAPKTWINYLQMFGDVLILNAAVALAFTIRHDWIYFLANTE
                     LHVVALIAFSVLMLLFFNLYGLYNTANKDWTDILSAVFVSLIFLSLGVAAITYMIPSY
                     IFPRRVLFTFGLLYGPAVALWRWLLLMIEHRITPAQKVIIVANPEEIPSLASKLNGNH
                     ILLGAITAQSFREAAYPNLGSAAEAEAIFTTYQPDIVLISGNLPEEIKSQIVLLGMTF
                     PCILYVVPNLYEIMVGQAVLDQFQDTPVYQIRLTRNPGQTQIKRLFDCLIAVTMLIIT
                     LPLTILAALAVKLTSPGPIFYTQERVGRDNRRFMLYKFRTMVKNAEAATGPVLAKRHD
                     SRVTKVGRFLRATRIDELPQLINVLKGEMSIVGPRPERPVFVERYNKEVPGYTYRHTM
                     DAGITGLAQVSGKYSTSPQDKLRYDLLYAKGASPLFDLRIMLQTLKVMLLKDKAS"
     gene            4525..>5342
                     /locus_tag="VFC74_06015"
     CDS             4525..>5342
                     /locus_tag="VFC74_06015"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HZK24925.1"
                     /translation="MNSYLILFSIAFFVTLLVTPLVAQLAFFIGAVDRPNRRKIHDTF
                     MPRLGGLAVFIGFAAAMIVLAMKEPKTLGLLLGGFMILILGIVDDIRGVSPRTKLLWQ
                     TITALAVVYLNLKLDMFSNPLNGLMQLGYLSIPLTVFWIISVTNAVNLIDGLDGLASG
                     VSAIALLTFAFIAYRSDQYLVALVSVILVGAILGFIRYNFFPAKIFLGDSGSLFLGFM
                     IAVLSAYGILKGATFYALIIPVIVLGVPIIDTCYAIVRRFYEKQPIFQPDKKHI"
BASE COUNT         1352 a         1378 c         1060 g         1552 t
ORIGIN      
        1 tacatttatt cactcctctt ggctaaggaa tatttaaact ctgacctctt gcttttacat
       61 ggcgatttag tctttgacga tgcggtgctc gccaataccc tggcctcacc ccagcccaac
      121 acagtgcttg tcaatcgcca gaccgccttg ccggaaaaag attttaaagc ccgtttagta
      181 aacggtgagg taaaagagat ctccattaag atttttggtg atgactgcgc tttcttaatt
      241 cccctctaca aattaagctc caccctaata caggcctggc tgatagaaat ggagcagttt
      301 gcagcccgta acgaactaaa tgtttatgcc gaaaacgcct taaataacct gctcgccgac
      361 tacgccctct cgcccgtcta tttccagcag gagctatgta cagagattga taacctacaa
      421 gacctggcca aggttaaagc cgctctggca gcaaggggag agcagtaagc atgaccatca
      481 gcgactatcg ccagctctgt caaaagaatg aagccgaaaa ctatttcttc gataaatata
      541 tctttcgcaa agtttctatc tatttcacca tcatctttat taaactgcgc cttactgcta
      601 atcaggccac ttttttaagt ttactggcct ctctgagcag ctgctacttt ttaatgcaga
      661 atacgactgc cgccatgttc ctaggccccg ccttgatctt cacctactac atgctggacc
      721 atgtggacgg cgaactggcc cgttactaca tccggcaagg taagcagcag ccttccctgc
      781 agggcactta ctttgatgtt ttaatccaca agtattctac caatctaatg ctctttttcc
      841 tgggtgtcag tgtctacaat ttttttaact accgctgggc agtactgctt ggctttgcgg
      901 cctgcatcgg catgagcgct ttccccaatc ttttggcctc acaggtaatc gtgcaaaaga
      961 ttgcccagca aaaagaatta gtagacaacc ctgctgtcgt agaagtactc aacttactag
     1021 agaaaaaaca ggaacaaatc aaaaaagtta ccagcactaa ccccttaaaa agattaaaaa
     1081 agatcatcac cgaactcctc tttttccctg gggccctgct gatgattatg ctagtaaccc
     1141 tcgccgatct catcgtgccc accttcaccc ttttcaacta tcccgttaat atccgcttac
     1201 tttttctgct cgccgtcaca ccgatttacc tgactaacgc cctccgccaa accctaaaat
     1261 ggttcagtaa atttgccgaa attgcttaac aaagagagga gggactaaca tgaatttaca
     1321 gcagtaccgt caactctgcc aaaaaaatga tagtgaaaac tatcttttag ataaactgat
     1381 ctttcgtaaa atctccattt ttatcaccat cttgttgatc aaactcaagc ttacttctaa
     1441 ccaaactacc ttcttaagcc ttttagcctc gctggccagt ctctattttc tcaccttgaa
     1501 tactcgcgcc gccttgctga ctgccgccgc cctcattttt acctattaca ttttagatca
     1561 tgtcgacggg gagctggccc gttatgctat tcaaaccgga caggctaaac ctaacctgaa
     1621 aggtcactac ttcgatgttt tagtgcaccg ttattcttcc aacctgatgc tcttttgcat
     1681 cggcctcagc atctacaacc attatggtta tcgctgggcc gtactgcttg gttttatggc
     1741 ctgtatcggt cttagctctt ttcccaatgt cattgccgcc caagtaattg ccggtaaaat
     1801 tgctaccaat agacaattag tccatactga ccctgccctc aggaagatcc ttgccgagct
     1861 ggaaacaaaa gagcagcaaa tcgctgctgt gcatagcggc agcttactac aaaaggtcaa
     1921 gaaactaatc atagaatctt tatttttccc cgggcatatc attgtactaa ttatagtcct
     1981 catcattgat gcctactggc ccggtctggc caccttaaat ctacgctgcc tgctgctggt
     2041 gggctttacc ctgttatata ccctcaaaac aatcattcaa agtatgctct ggctcgctaa
     2101 actaaaaaca atcgcttaat ctgaatggta ggatgtggca cttatggaca aacaactgca
     2161 agagatcctc tcatatttcc accaagacga gcttgcctac tggctggaca gcggcaccct
     2221 gctggggctg atgcgcgaag gtcagcttct cgccagtgac aaagatttgg atatcagcat
     2281 ctgggataca gagcttgccc aactacaagc cctcctgccc agttttcgcc aagctggcta
     2341 ccaaatctat gctgcctctt accaagggca aatctttaaa tataatctta cccccagcaa
     2401 cctaaaaaag cttcgcactg tagatatcaa catctaccgc agcaataaca accacgcctg
     2461 gtgccctatg tactacttta aattaagccc cacgacagaa aagaagccgc caagcaaagc
     2521 atcaccacta aaagcactac gcagcttact gcgcagtacc tggaaagcta tcaacaccaa
     2581 atttaccatg aacgtcaaaa tagaccgcct gccctggcag ccttttctca ttatcggcac
     2641 ttggtggatt ccacaagctt tctacgacaa gcgcatcttc aatgaacagt accaagtgtg
     2701 gatccctgcc gcctggcagg actatctggc tttccgctac ggcaactggc aagagccgca
     2761 aactgattgg gtcttctacc gtgacgacca aggtattaac ccgcaatcac cacagcagct
     2821 gctcaataac taaacccgtg agggaacttt accaccacgc tgacgtcaaa aagagttgtt
     2881 tcaaatgcct agcttataaa gatagagaac ggttaaccgt tccgatgtaa atagagggga
     2941 tgggtcaaat gggtactaat gcgccaaaaa cctggattaa ttacctgcag atgtttggtg
     3001 atgtgttaat tctcaatgcg gctgtggcct tggcttttac catacgccac gactggattt
     3061 acttcttagc caacaccgag ttgcatgtgg tggctttaat tgctttttcc gtcttaatgc
     3121 tcttattctt taatttatac ggcctttata atacggccaa taaagactgg acggatatcc
     3181 tgtccgctgt ctttgtttct ctaattttct taagtttagg cgttgccgcc attacttata
     3241 tgattcccag ctacattttc ccccgtcgtg tcctgtttac ttttggcctt ttatacggac
     3301 ccgcagtagc cctttggcgc tggctgctct taatgattga acaccgtatt acgccagcgc
     3361 aaaaggtaat tattgtggct aaccctgaag aaatcccgtc tctggccagc aaattaaacg
     3421 gcaaccacat tcttttaggc gcgatcactg cacaaagttt ccgtgaggcg gcttacccta
     3481 atttaggcag tgctgctgaa gcggaagcca tctttaccac ttaccagccg gatattgtct
     3541 tgatctctgg caacctgcct gaggagatta aaagtcaaat cgtgctcctc ggcatgactt
     3601 tcccttgcat tctgtatgtc gtccccaacc tttatgaaat catggtgggg caagcggttt
     3661 tagatcaatt ccaagacacc ccggtttatc agattcgcct cactcgtaat cctggtcaaa
     3721 cacaaatcaa acggcttttc gattgtctaa ttgccgtgac catgctgatc attacgcttc
     3781 ctctcactat actagctgcg ctggccgtta aactcacttc accgggtccc attttttaca
     3841 cgcaagagcg cgtgggccgc gataacagac gctttatgct ctataagttc cggacgatgg
     3901 ttaaaaacgc tgaagctgct accggcccag tcctggctaa acgtcatgac tcgcgcgtca
     3961 ccaaagttgg ccgcttcctg cgtgccacca gaattgatga actgccccaa ttaattaacg
     4021 tgctcaaagg cgagatgagt attgtaggcc cgcgccccga acgccccgtt ttcgtggagc
     4081 gctataataa agaagtgccc ggctacacct accgccatac tatggatgcc ggcatcaccg
     4141 gtctcgccca ggtgtccggc aaatacagca cttcaccaca ggataagctg cgctatgact
     4201 tactctacgc caaaggagcc tcaccgcttt ttgacctgcg gatcatgtta caaaccttga
     4261 aagtaatgct gttaaaagat aaggcttctt agcttactac tatcataaaa gatgagtata
     4321 tgtgctttca gatgctcatc ttattgtcac gcttacctga aatgatcttc cttttaagtt
     4381 agcacatact ggcactactt tcactatttt gccagttacc ctagaattgg cattgtcaga
     4441 aactgagttc ttttgtataa tgagtaagat agtgttggcc gtactgtggc tttttgtcat
     4501 taactgacac gggagattga ttttatgaac tcatacctga tacttttttc gattgctttt
     4561 tttgttacgc tattggttac gccgctggtc gcccagctcg ccttttttat tggcgccgtg
     4621 gacaggccta atcggcgtaa aattcatgat acatttatgc ccaggttagg cggactagca
     4681 gtttttattg gttttgctgc cgcgatgatc gtcttggcga tgaaagagcc caagacttta
     4741 ggtcttttgc ttggtggttt tatgattctt attttaggaa ttgttgatga tatccgtgga
     4801 gtttcacctc ggactaaact cttatggcaa actattacgg cgctggcggt agtttatctt
     4861 aatcttaaac tggacatgtt tagtaatcct ttaaatggtc tgatgcaatt aggttaccta
     4921 agtatccctc tgaccgtttt ctggattatt tctgtgacta atgctgtcaa cttaattgat
     4981 gggctagacg gcttagcctc aggtgtttcg gcgattgcgt tgctcacttt tgcttttatc
     5041 gcctaccgca gcgatcaata tcttgtcgcc ctcgtttccg tcattttagt aggggcgatt
     5101 ttaggtttta tccgctacaa ttttttcccc gccaagattt tcctcggcga ttccggctct
     5161 ttatttttgg gctttatgat cgcggtcctt tccgcttacg gcatccttaa aggggctaca
     5221 ttttatgctt taatcatacc cgttattgtg ttaggcgtgc cgattatcga tacctgctat
     5281 gctattgtgc gtcgctttta tgaaaaacaa ccgatctttc agcccgataa aaaacatatc
     5341 ca
//
LOCUS       DASRMS010000131        18911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_32128, whole genome shotgun sequence.
ACCESSION   DASRMS010000131 DASRMS010000000
VERSION     DASRMS010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 18911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18911
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32128"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..1453)
                     /locus_tag="VFC74_06020"
     CDS             complement(92..1453)
                     /locus_tag="VFC74_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018595248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK24926.1"
                     /translation="MKKNIPMLYFIAIVGQMFGTAIAGTYLSFYMTERLLLTASAMGV
                     ILLVARIVDLIIGFLSGMIVQKTSFKYGQYRSWLIIGPICVAIGVIMCFLNPNISVQM
                     KGVIVLLGYILYGGGMSFAQLGQNGMMSKIAGPNLDMRLTISGKIVQGQTVAQIATAL
                     TIMPLIIWVERFGVDGYTVVAILFSVISIAAQTTLFKGTKEYDQYDPDFKSKSGASGS
                     VGSMIGDILKNKQILLVMLGDTLRFAAMMTIISLAMYYFTFVAGNPLLISVAMLSQSI
                     AGFTGALFAPSIVKKIGKKNAAVTSGAIATLAYTGLALFAGANPILYIVLNSAGFLGM
                     SLVNSCGVNLYLDCAEYQLYKTGKDNKTFSMSMFGVAIKVGFAVTSILVSWVLQTSGY
                     DAATGMAEPARLARLIGAVPAVLYLVYMLIYMFFYKITEEKSREYAAHNYEKAAAAQK
                     ANN"
     gene            complement(1909..2823)
                     /gene="ftsY"
                     /locus_tag="VFC74_06025"
     CDS             complement(1909..2823)
                     /gene="ftsY"
                     /locus_tag="VFC74_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HZK24927.1"
                     /translation="MGLFNRLKAGLQKTRSNILGRLGDLFSRNHFDDEFYEELEEILI
                     GADVGVETTLEVVENLRAQVKAAKATEAEQVMDILKKLLLELLGSTTIPLQMAPEKPT
                     VILVLGVNGVGKTTTIGKLAASFYAQDKKVLLAAGDTFRAAAIEQLEVWASRAHSEVV
                     KHQQGADPSAVLFDSVNAAKARDCDVLLCDTAGRLHNKTNLMEELKKVRRVIEKALPG
                     APHEILLVLDATTGQNALAQAKVFNEVTPLTGVVLTKLDGTAKGGIVIAIARELKVPV
                     KFVGVGEGIEDLQPFDPADYVEGLLTLN"
     gene            complement(2889..6467)
                     /gene="smc"
                     /locus_tag="VFC74_06030"
     CDS             complement(2889..6467)
                     /gene="smc"
                     /locus_tag="VFC74_06030"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HZK24928.1"
                     /translation="MHVKRLEIHGFKSFADKTIFELTPGVTVIVGPNGCGKSNITDAI
                     RWVLGEQSARHLRGFRMEDIIFSGTSKRKALSFAEVSLTFDHSDGALGLEYEEITVTR
                     RLYRNGESEYLLNKNTCRLKDILELFMDSGVGKEAYSFIGQGRVDEIINARPEERRQI
                     FEEAAGILKYKSRKREAERRLAETAENLLRVGDIIHELSAQLPPLKEQAEQAQSYLQL
                     REQLKAQEIDLLVHDALNLRQRWYGLDNKAKAAADDLIGQQTTVNRQEAELTTSQLLL
                     DKEQTTVASRQQETQQLASEIEKAQAQIAVTKEKIHSAERQLKEASLYMEDLDQQAAG
                     LTETQEQLAQQLQAINDTKQQAEAQLAKAQAALAELETSPDVQQSLTKQAELDKLLPT
                     LHRLQRELDRVSMEVEQLEAQGEALAAQETVKQAEAKSLTEHGQALLRQKEMIRGAKE
                     QQTSEQAELEQNLQTLQVQQEQLSRQSQQQQKELTATQNKLAVLQELEQAMAGYYQGV
                     KTILKVRKKQAQLQGIVGTVADILQVPPRYVPAVEAALGASLQNLVAENEQVAKAAIT
                     YLKQTKGGRATFLPLNILEVATRRPASDKVTQIDGYLGIAADLVQTQPRFQQVIESLL
                     GRVHFVANLDTALPAAQALHYRERVVTLDGDIIAPGGAMSGGFDKKQGGVLTRRKEAA
                     ALKEKLEQQQTVLLSLQTKEKSSQEQQAAAKQQLAELQQKLQQTELQFSLNEQEIVYW
                     ARQEQELTVTLQKLQQELTAVQETLSVRREVIKQTVQELTTVQKQEHALRIELAELEG
                     RLSAREIEKQSLREHYTECRVRVASLQKQQERYADEADRARRERSLLENKKQHKAAQI
                     SLVQAAQQQLNVDIAAKQITVDTLQNKRSQAVAVLAKREQALKTMATELREHTAQLRQ
                     LEKSFAGLERKQARLEVERERVEGDLQAVLNRLRDSWELEFAEAEKAAKPLQDRQAAQ
                     TAISQIKESVNKLGTVNLGAIEEEQRVAERVGFLTTQRDDLREGERDLLRIIKEIDNR
                     MGEKFASSFALINENFAQVFKELFGGGRAELCLIDPDNPLEAGVDIVAQPPGKKLQHL
                     SLLSGGEKALTAISLLFAFLKMRPTPFCILDEIEAALDEANLSRFSDYLRTYSAQTQF
                     ILITHRKRTMEQADILYGVTMEESGVSKLISVRLRTSNNAEPNLSA"
     gene            complement(6650..7354)
                     /gene="rnc"
                     /locus_tag="VFC74_06035"
     CDS             complement(6650..7354)
                     /gene="rnc"
                     /locus_tag="VFC74_06035"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004066068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HZK24929.1"
                     /translation="MKKADFQAIFTLLQIEPKEWQLYQQALTHSSYAHEHDLGSHAYN
                     ERLEFLGDAVLELAVSEELYRRYPQLPEGKLTRFRAGLVCEDTLYRLANKLELGNYLR
                     LGKGEAANGGRQRPSLLADAFEAVLGAIYLDLGFAQAKAAVQKQFAPLYGDLQQGSLP
                     FDYKTLMQEYTQAKMAATPEYRIVAETGPDHDKVFVAQVMIKEKVLGKGSGHSKKEAE
                     QEAARVAYERIKKDKS"
     gene            complement(7375..8616)
                     /gene="fabF"
                     /locus_tag="VFC74_06040"
     CDS             complement(7375..8616)
                     /gene="fabF"
                     /locus_tag="VFC74_06040"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HZK24930.1"
                     /translation="MKRRVVITGLGMVTPVGTGKTIFWDNLLAGKSGIDRVQRFNVDD
                     YPTQVAAEVKDFNPADYMDKKESRRMDRFSQFAVAAARLAMADAKLESSEIDNQRVGV
                     SVGSGIGGITTIQEQVQVLINKGPRRVSPFFVPMLIANMASAYISIEFGLKGPNTTIV
                     TACATGTHSVGEAMKTIQLGAADVMVAGGAEAALVPVAFAGFCALRAMSSCNDDPQRA
                     SRPFDSERDGFVMGEGAGIVILEELEHALKRGAKIYAEVAGYGLSSDAYHITAPDPDG
                     AGAQLCMSMAIKDAGLRPEDVDYINAHGTSTQLNDKTETQAIKRVFGEHAYDLAVSST
                     KSMTGHLLGAAGGIEAIICALAIENGMIPPTINYEHPDPECDLNYVPNQAIARPVQTA
                     MSNSFGFGGTNASLIIKKFSS"
     gene            complement(8688..8915)
                     /gene="acpP"
                     /locus_tag="VFC74_06045"
     CDS             complement(8688..8915)
                     /gene="acpP"
                     /locus_tag="VFC74_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HZK24931.1"
                     /translation="MDVLSKVKGLISDQLGADEANIALETSFEELDADSLDIVELVMA
                     IEEEFSLEISDEEVENIKSVGDVVRYIEANA"
     gene            complement(8967..9716)
                     /gene="fabG"
                     /locus_tag="VFC74_06050"
     CDS             complement(8967..9716)
                     /gene="fabG"
                     /locus_tag="VFC74_06050"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HZK24932.1"
                     /translation="MRLVNKVAIVTGASRGIGRAIAIALAREGAKLVINYTANAQAAE
                     DTLAAVQEAGSQGLLVQADVTAAADCNKLVKAAQEHFGKIDILVNNAGITRDNIAARM
                     KADEWQAVLDTNLTGAFNCAKAVIRPFLKQKTGGRIINMASIVGKIGGIGQVNYAAAK
                     AGLMGMTKSLARELAARQVTVNAVAPGYINTAMTAVLSSEVKDNIVKQIPLGRTGEPE
                     DVAALVVFLASDEAAYITGQVIAIDGGLVMC"
     gene            complement(9700..10665)
                     /gene="fabD"
                     /locus_tag="VFC74_06055"
     CDS             complement(9700..10665)
                     /gene="fabD"
                     /locus_tag="VFC74_06055"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HZK24933.1"
                     /translation="MGKIAFIFPGQGAQYIGMGRELAEHYPVAREVFTAADEALSMKL
                     SDIIFDGTDEQLKQTEITQPAIVATSLAFLAVLKQHGLVPDAAAGLSLGEYSALVAAG
                     ALSLQDALPLVQKRGQLMQEAVPPGVGGMAAILGLAREKVATVCQLASEVGIVEPANF
                     NAPGQIVIAGELSALRRACELAKEHGARRTVELPVSVSFHCSLLQSVEPLLAAELDKV
                     QIKPVEIPVITNVTAAYVNSTAAIRDALVRQVSHSVLWQDSMEKLIADGFNRFVEVGP
                     GKSLTGLMKKTNTDVWVHQMEDRKTLAAVLEALGAGEGENIAFSQ"
     gene            complement(10687..11634)
                     /gene="fabK"
                     /locus_tag="VFC74_06060"
     CDS             complement(10687..11634)
                     /gene="fabK"
                     /locus_tag="VFC74_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HZK24934.1"
                     /translation="MVLKSKLCDLLGIQYPILQGGMAWVATGELAAAVSETGALGIIG
                     AGNAPAAVLEQEIRKAKARTDKPFGVNIMLLSPYVEEVVELLCREKIRVITTGAGNPG
                     RYVKLFQENGAKVIPVVSSVALSKRLLRLGVDAMIAEGMEAGGHIGELTTMALVPQIV
                     DAVNVPVIAAGGIADGRGVAAALALGADGVQLGTRFISAYECTAHAQYKGMILKAKDR
                     DTVITGVSTGHPVRVIRNNLTREISRLEKEGASLAEIDKFAVGRLSDAVRRGDVVNGS
                     VMAGQSAAMVTKEESAQDIINDLIKQTRSVISELAKFIG"
     gene            complement(11648..12631)
                     /locus_tag="VFC74_06065"
     CDS             complement(11648..12631)
                     /locus_tag="VFC74_06065"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006440054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HZK24935.1"
                     /translation="MKLQDAGILGVGSAVPPKIVTNSDLEKIIDTSDEWIRTRTGISE
                     RRVVEDGVFTSDLAAQAAEKALQAANTTAEEIDLIIVATITPDTIMPSTACFVQAKLG
                     AKKAFAFDLSAGCTGFIYALSVGRLYIQSGLYRRVLVIGAEVMSKFLDWSDRSTCILF
                     GDGAGAVVLGPVEEPGFINVTLGADGSLAELLVVPAGGSQLPASQQSVAERQHYIKMN
                     GHETFKFAVRVMGEVTQQALDAAGLVKEDLDFLVPHQANLRIIESARKRLKLPPERVI
                     VNIDRYGNMSSASIPVALDEAIQQGRLKRGDLMALVGFGAGLTWGAALVRY"
     gene            complement(12667..13686)
                     /gene="plsX"
                     /locus_tag="VFC74_06070"
     CDS             complement(12667..13686)
                     /gene="plsX"
                     /locus_tag="VFC74_06070"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HZK24936.1"
                     /translation="MKLVIRVALDVMGGDHAPKQIIAGGLLALTELADLHIHFVGPQA
                     VLENYLRDKVYDQNRVALMPAEEIIKNEDSPVLAVRRKKNSSMMVALQMLRDKKVEAM
                     VSAGNTGALMAGSLLLTGRIEGIERPALTVVAPTFPGGNVVLLDVGANMDAKAEQLLQ
                     YGIMGKIYAREVLLKKEPRVGLLNVGVEEIKGNEQVKKAYQLMKEKLPCFIGNVEARN
                     VLDGVADVIICDGFSGNIILKTLEGLVAGLFGSLKEAMSANLKSKIAASLLKPELLAL
                     KAKLDYTEYGGAPFLGVGGACIKGHGSSNARAIRSAIVNQAYLYARQNVNGLIEQELA
                     ALLTD"
     gene            complement(13790..13966)
                     /gene="rpmF"
                     /locus_tag="VFC74_06075"
     CDS             complement(13790..13966)
                     /gene="rpmF"
                     /locus_tag="VFC74_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HZK24937.1"
                     /translation="MAVPKRKTSKSRKNKRRTHWKLTVPGLISCPQCHEPKLPHRVCV
                     HCGYYKGREAVEIK"
     gene            complement(13984..14448)
                     /locus_tag="VFC74_06080"
     CDS             complement(13984..14448)
                     /locus_tag="VFC74_06080"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HZK24938.1"
                     /translation="MKIELQALKDAPGKDITYNFTVAESALNLDEEDLQVIAPLEVQM
                     SAAYRDRKVEVRGSLRTRVILTCSRCLKQVPISFQEEFEDEIPVSEEKEINLSELVRE
                     LFITTLPFKPLCQAACQGICPTCGTDLNEKQCSCPVDNMDPRLSVLKKLLDK"
     gene            complement(14552..15592)
                     /locus_tag="VFC74_06085"
     CDS             complement(14552..15592)
                     /locus_tag="VFC74_06085"
                     /inference="COORDINATES: protein motif:HMM:NF017200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HZK24939.1"
                     /translation="MKQLRKIIAIIVVGALLLFAKTGYLLIKPGSAEDLSRFVTVQGS
                     TDDDNGTFYLVTVSQQRATPVLLCYALLHPDVELQPSTSVIPPNMDRQEYNELMQRWM
                     TESQNLAKVIALRHSGYEVPIHSDGVEVVDVEEDSPAQGLLFPEDVIVAVDGHSVYLA
                     EELISQVQLRPVGDPVSLTVKRNGEQREVEVATTNHTELPERAAIRVLIQTLNWQPVL
                     PLEIEIDAGEISGPSAGLMFVLEIMRQLEARDLTAGHKIAGTGTINLLEEVGAIGGVH
                     QKVKAAEKAGAAYFFVPLENYEDAKQAAQKITLVPVQTLTEALHFLQELTPAEKSASN
                     CLPVHKKGSKAA"
     gene            complement(15576..16385)
                     /locus_tag="VFC74_06090"
     CDS             complement(15576..16385)
                     /locus_tag="VFC74_06090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HZK24940.1"
                     /translation="MFKRGKKVGLALGGGSARGFAHIGVIKVLKLAGVPIDLITGTSM
                     GSVVGAAYCAGLELPLLERLFSHADSKLWLDWTFPHMGLAAGDKLEQLMLLLTRRKTF
                     DQLEVPLAIVATDLCRGEKVVMQEGLVARAVRASAAIPGIFRPLEEGEQVLVDGAVIE
                     RVPAPTARTLGADLVIAVDVGIYLDGHKPQHILDVITQSLDVMQRDLSRYSLEAADVV
                     ISPALREIAPGHFHRAKEAIKAGEEAAHEALPSIRQMLEKEGLWKLNETIT"
     gene            16470..17711
                     /gene="ylbJ"
                     /locus_tag="VFC74_06095"
     CDS             16470..17711
                     /gene="ylbJ"
                     /locus_tag="VFC74_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YlbJ"
                     /protein_id="HZK24941.1"
                     /translation="MNTKRFNRDRVPAYLLGIAAVALAVALVLHPSEAFDAAVKGLEV
                     WWNVVFPALLPFFIGSEILMGLGVVHFLGVLLEPLMRPVFNVPGVGSFVIAMGLASGF
                     LVGGNLSAQMRRKSLISKVEAERLVSFTNTSDPLFMCGAVALGMLGYPEIGGTIVAAH
                     YLSALTTGLFLRFYGRNQPNTPETFERGRMLSRAATALYHARKKDGRAFGQLMGDAVR
                     NSVNNLLLIGGTIILFSVIIRILTVTGVVGIISAAAYKILFGPLGMHPDLMPAVVNGF
                     FEIDLGCLVASQVSTAVPMIQKIMVTSGIIAWSGLSVFAQIAAITSDTDMKMKPFVIG
                     RMIQAFFAVLYTWLFMGPLAPVMSRAMPVFSHLHPPVGMTVLQSLGYMSWLFIILAAG
                     LCLIGLLLNIFKGIKISVFRR"
     gene            complement(17701..18183)
                     /gene="coaD"
                     /locus_tag="VFC74_06100"
     CDS             complement(17701..18183)
                     /gene="coaD"
                     /locus_tag="VFC74_06100"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003493233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HZK24942.1"
                     /translation="MIVVIYPGSFDPVTNGHRDIIDRASRVFSKVIVTVLDNPRKAPM
                     FSIEERVEMLKLVVKNYPNVEVDSFQGLLVDYAKMKQTKVMLKGLRPMGDFDFEFQMA
                     ITNRQLDPELETLFMATTNRYSYISSSVVKEVGIFGGDISEFVPPEIYELVMKKVVTV
                     "
     gene            complement(18206..18772)
                     /gene="rsmD"
                     /locus_tag="VFC74_06105"
     CDS             complement(18206..18772)
                     /gene="rsmD"
                     /locus_tag="VFC74_06105"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HZK24943.1"
                     /translation="MIALRIIAGCARGRQLKTRKGSPTRPTADRVKESCFNILSNRLQ
                     GAYFLDIFAGNGGIGLEALSRGAEKCVFIEKNAHCAKIITGNLFLCGLQAGGEVLQLD
                     ALTALAQLKIKQQQFDIIFLDPPYHSPVLASVLRQLAANDLLAPAGIVVVEHHRQHSD
                     WYQPEEWQTNRVKKYGDTILTFLTPSKA"
BASE COUNT         4824 a         4862 c         3797 g         5428 t
ORIGIN      
        1 ttacatcttc agctagtgct cactagcaat aagcatagtt agtacattaa tgataggatc
       61 tggccggcag cgtgcgcctg ctaactagct attagttatt ggctttttgt gcagctgctg
      121 ctttttcgta gttatgggca gcatactcac gagatttttc ttcggtaatc ttatagaaaa
      181 acatgtaaat caacatgtag accaagtaaa gcactgccgg caccgctcct atcagtctag
      241 ccaaacgcgc cggttcagcc ataccggtag ctgcatcgta accggatgtc tgaagcaccc
      301 aagaaaccaa aatcgaagtt accgcaaaac caaccttaat agccacaccg aacatagaca
      361 tggagaaagt cttgttatcc ttgcctgttt tgtaaagctg gtactcagca cagtccagat
      421 acaggttgac tccacaggag ttaaccagag acatgcccag gaagccggct gaattcaaca
      481 caatgtacag tatagggttg gcgccggcaa aaagcgctag tcccgtgtag gctaaggtgg
      541 caatggcacc tgaggtcaca gctgcattct tcttgccaat ttttttaacg atggacgggg
      601 caaataacgc accggtaaac cctgctatac tctgtgaaag catcgccacg ctgataagca
      661 gcgggttccc tgcaacgaaa gtaaaataat acatagccaa agatataatc gtcatcatgg
      721 ctgcaaaacg taaagtatca ccaagcataa ccagaagaat ctgtttgttc ttaagaatat
      781 ccccgatcat ggagccgaca ctgccactgg cgccactctt gcttttgaag tctggatcgt
      841 attgatcata ctcttttgta cccttaaata atgtcgtttg tgccgcaata ctgatgacgg
      901 aaaagaggat tgccaccacc gtataaccat caaccccaaa gcgttccacc caaataatga
      961 ggggcataat agtaagcgca gtagcaatct gagcaaccgt ctgaccttga acgattttac
     1021 ctgaaatcgt caaacgcatg tccaggttag gaccggcaat cttagacatc atgccatttt
     1081 gtcctagctg ggcaaaactc atgccgccgc cgtagagaat atacccgaga agcacaataa
     1141 cccctttcat ctgtacagag atgttgggat tgagaaaaca cataattact ccgatagcaa
     1201 cacatatggg accaatgatc aaccaagaac ggtactgacc gtatttaaac gatgtttttt
     1261 gtacgatcat accggaaagg aaaccgataa tcagatcaac aattctggcc accaagagaa
     1321 tcacgcccat ggcggaagcg gtgagcagta atctttctgt catatagaaa gaaagataag
     1381 tccctgcaat agcagtgccg aacatttgcc ctacaatagc gataaaataa agcattggta
     1441 tatttttctt cattttctaa acctcctaaa tattctaaca attttagcaa ctcttatcct
     1501 cattactaca atcttcagca ggacgtaaat gaagttcagc aaccatctcc tcgcatcaag
     1561 ctaatttcaa gagttctcca caagcaaacc tttcctggca tctcctcgca gtaacactat
     1621 gagtttcgtc tgctggctct aatgaaaaaa taatagacaa catcaaaccg cctgaggtaa
     1681 atctcttgtt tcacaagcac agctctgtac aagctgagcc agtcaattta agtataaatg
     1741 taaattagct caatgtaaac cattattttt tcgtcgcgcg ctgctaagat tacaaaaaac
     1801 gactccgata cacacttatc ttgatgtaaa gatactgtga tctgctccct ggagtcgttt
     1861 tttgcggcag attttcagtt atttagacct ccttgtggaa aaagacaatt agttcagagt
     1921 taacagtcct tccacatagt ccgcgggatc aaagggctgt aaatcttcta tcccttcacc
     1981 aacgccaaca aatttaactg gcacctttaa ctcacgggcg atggcaatga caatgccgcc
     2041 cttggcggta ccatcaagtt tggtcaggac aactcctgtc agcggggtaa cttcattaaa
     2101 gactttggcc tgagctaagg cattttgccc tgtggtggca tctaaaacca gtagtatttc
     2161 atgcggtgcg cctggcaaag ccttttcgat tacccggcgc acttttttta attcctccat
     2221 taaattagtt ttgttatgga gacggcctgc tgtatcacaa agcagcacat cgcagtcccg
     2281 tgctttagca gcattaacac tatcaaagag taccgctgaa gggtctgcgc cctgttggtg
     2341 tttaactact tcgctatgcg cgcgtgaggc ccagacttct aattgctcta tggctgcagc
     2401 ccggaaagtg tcaccggcag caagcagaac ttttttatct tgagcataaa aactagcggc
     2461 taacttgccg atagtggtcg tttttccaac cccgtttacc cctaacacta aaataacggt
     2521 cggcttttct ggtgccattt gcagtggtat cgtggtgctt cccagcagtt caagcagcag
     2581 ctttttcaga atatccatca cttgttcggc ttcagttgct ttggctgctt taacttgagc
     2641 acgaagattt tctactactt ctaaagttgt ctctacgcca acatcggcgc caattaatat
     2701 ttcttccaat tcttcataaa attcatcatc aaaatggttg cgggagaata agtcccccaa
     2761 gcgacccaga atattgctgc gtgttttttg taatcctgct ttcaggcgat taaataatcc
     2821 catctccgcc ctcactcctt tcccgatcta ctattgttag ccttttctta cttcgtgctg
     2881 ctcatacctt aggcactaag gttaggctcc gcattgttac ttgtacgtaa acgcacggag
     2941 attagttttg aaactccgga ttcctccatg gtgacaccgt agagaatatc agcttgctcc
     3001 atagtacgct tgcggtgcgt aattaaaata aattgagttt gtgcgctata tgtccgcaga
     3061 taatcactaa aacgtgataa attggcctca tccaaagccg cttctatctc atccaaaata
     3121 cagaacggtg ttggcctcat tttcaaaaag gcgaacaata aagagatagc cgttaaagct
     3181 ttttcccctc cggagagcag cgataagtgc tgcagtttct tgccgggtgg ctgcgccaca
     3241 atatctacac cagcttccaa aggattatca ggatctatga gacataactc tgcccgtccc
     3301 ccaccaaaca attctttaaa cacttgcgca aagttttcat taattagggc aaatgagctg
     3361 gcaaatttct ctcccatgcg attatcaatt tctttaataa tccgcagcag atctctttct
     3421 ccttcacgca aatcatctcg ctgcgttgta agaaacccca cacgttctgc tacacgctgc
     3481 tcttcctcaa tggcgcctag gttaacggtc cctaatttat taacactttc tttgatctgg
     3541 ctaatggccg tctgcgctgc ctgcctgtcc tgcagcggct tggctgcttt ttccgcttcg
     3601 gcaaactcaa gctcccaact atctctcagt cgattaagga ctgcctgtaa gtcgccttct
     3661 acgcgctcac gctcaacttc taggcgtgct tgtttgcgtt ccaaccccgc aaaggatttt
     3721 tccagctgac gcagctgcgc tgtatgttca cgcagctcgg tcgccattgt ttttaaggcc
     3781 tgctcccgtt tagctaaaac agctacggct tggctgcgtt tattttgcaa agtgtctact
     3841 gttatttgtt tcgccgctat gtccacgttt aactgctgct gcgctgcttg caccagtgaa
     3901 atttgcgctg ctttgtgctg ctttttattc tccagcaggc tgcgctcacg acgggcacgg
     3961 tcggcttcgt ctgcgtagcg ctcctgctgc ttttgtaagg aggcaacacg tacacgacac
     4021 tctgtatagt gctcacgtag actttgcttt tctatttccc gtgcggatag cctgccttct
     4081 agttcagcta attctatgcg cagggcgtgt tcctgttttt gtactgtagt tagttcttgc
     4141 actgtttgct taatcacttc tctgcggact gagagagttt cttgcactgc tgtaagctct
     4201 tgctgcaatt tttgcaaagt cactgttaat tcttgctctt gtctggccca atacactatt
     4261 tcctgctcat ttaagctgaa ttgtaattct gtttgctgta gtttctgctg cagttcagca
     4321 agctgctgct tggcggcagc ttgctgctct tggcttgatt tctcctttgt ttgcagagac
     4381 aaaagtacag tttgctgctg ctcaagtttt tctttcagcg ctgctgcctc tttgcggcgc
     4441 gtcaggacac cgccttgctt tttatcaaag ccaccactca tcgcaccacc gggggcaata
     4501 atatcaccat ctaaagtaac tacccgctca cggtaatgca aagcttgggc tgccggcaac
     4561 gctgtatcta aattagcaac gaaatggacg cgccctaata aggattcgat tacctgttga
     4621 aagcgtggct gtgtttgtac taaatcagct gcgatgccaa gatagccgtc aatttgcgtg
     4681 accttgtcgc ttgccggccg tctcgtggct acttctagga tatttaaggg caagaaggtt
     4741 gcccgaccgc cttttgtttg ctttaagtaa gtaatagctg ctttagccac ctgttcattt
     4801 tctgcgacca aattttgcag cgaagctcct agcgctgcct ctacagcggg gacatagcga
     4861 ggaggaacct gcaagatatc ggcaaccgtg ccgacaatac cctgcagttg agcctgcttt
     4921 ttacgtacct tgagaattgt tttaacgcct tgatagtagc cggccatggc ctgctccaat
     4981 tcttgcagca cagcaagctt attttgtgtc gcagtgagct ccttttgttg ctgctgagac
     5041 tgtcgggata gctgttcttg ctgaacttgc agagtttgca agttttgctc taattctgcc
     5101 tgctcactcg tctgctgttc ctttgccccc ctgatcattt ccttttgcct caaaagagcc
     5161 tggccatgtt ctgtcaaact tttcgcctct gcttgcttga cagtctcttg tgcggcaaga
     5221 gcttccccct gcgcctcaag ttgttctacc tccatactaa cccggtcaag ttcacgctgc
     5281 aggcggtgca gtgttggcag taatttgtct aactccgctt gtttagtcag ggattgctgc
     5341 acgtctggtg aagtttccag ctccgcaaga gccgcttgag cctttgccag ttgtgcttct
     5401 gcctgctgtt tagtatcatt aatggcctgc agttgttgtg caagctgctc ttgtgtttct
     5461 gtcaagccgg ctgcttgctg gtctagatcc tccatatata aactggcttc ttttaactgt
     5521 cgctctgcac tatgtatttt ctctttagtt acagcaatct gtgcctgtgc tttttcgatt
     5581 tcagaagcga gctgttgtgt ttcttgctgc ctggaagcta cggtggtttg ttctttatct
     5641 aataacaatt ggctggtggt gagttctgct tcctgccgat tgacagttgt ttgctggcca
     5701 attaaatcat cagcggctgc tttagcttta ttgtctagcc cataccaacg ttggcgcaaa
     5761 tttagggcgt cgtgtactaa gagatcaatt tcctgtgcct tcaattgttc tcgcagctgc
     5821 aggtagcttt gggcttgttc agcctgctcc ttcagcggcg gcaattgtgc agacaactca
     5881 tggataatat ctcccacccg caaaaggttt tctgctgttt ctgccaggcg acgttctgct
     5941 tctcgtttgc gacttttata tttgagaatt ccggcagcct cttcaaagat ctggcgtcgt
     6001 tcttctgggc gggcattaat aatttcgtca acacggcctt gtccaataaa agaataggct
     6061 tctttaccca caccggaatc cataaataat tccaggatat ctttcagtcg acaggtgttt
     6121 ttatttagca gatattcact ttccccattg cgataaagcc gacgggtgac tgttatttcc
     6181 tcatattcta accctaaagc gccatcacta tggtcaaagg ttagggatac ttcagcaaag
     6241 cttaatgctt tgcgcttgga ggtaccgctg aagataatat cttccatacg aaaaccccgc
     6301 aggtgtcgcg ctgactgttc acccaaaacc cagcgtatag catccgtaat attactttta
     6361 ccacagccgt tgggcccgac aattactgtc acaccaggtg ttagttcaaa tattgtttta
     6421 tcggcaaatg atttaaaacc atgtatttca agacgcttaa catgcaatga ttttcccctc
     6481 ctcctgacat tggtgggctt actgtattta caatactata acatatctaa tttctctatc
     6541 tgtataaaaa tacctgcttg ttgacaagca ggttaagtta agcaaattag attacagccc
     6601 agatttttca agacaagctc attcataaga ttacgaaaca gctttcaggt caagatttat
     6661 cttttttgat ccgctcgtaa gccacccggg ctgcttcctg ttctgcttcc ttcttggagt
     6721 gtccacttcc cttacccagg accttttcct taatcattac ttgcgctaca aagactttat
     6781 catgatctgg tcctgtttcg gccacaattc tatattctgg tgtagctgcc attttggctt
     6841 gtgtatactc ttgcattaat gttttgtaat caaaaggcag actaccctgc tgtaaatctc
     6901 cgtaaagcgg tgcaaactgc ttctgcactg cagccttagc ctgggcgaac cccaggtcta
     6961 aatatattgc acccagtact gcttcaaagg catctgccag taaagaaggg cgttggcggc
     7021 caccattagc cgcctcgcct tttcctaaac gcaaatagtt tcccagttcc agcttattgg
     7081 ccagtcggta taaagtatct tcacagacta aaccagccct aaagcgtgtt aatttacctt
     7141 ctggcagctg aggatatcgt ctgtacaatt cttctgaaac cgccagctcc aaaaccgcat
     7201 cgcctaaaaa ctctagacgt tcgttataag catggctgcc caaatcatgt tcatgggcat
     7261 aggaagagtg ggttaacgct tgttgataca gctgccactc tttaggctcg atttgtagta
     7321 gcgtaaatat tgcttggaaa tcagcttttt tcataccttc accgttataa ttccttaact
     7381 actaaatttt ttaattatta aggaggcatt agtgccgcca aagccaaaag aattggacat
     7441 cgcagtttgt acggggcggg ctatcgcctg attaggtaca taattcaagt cacactctgg
     7501 gtcaggatgc tcgtaattaa tggtaggcgg tatcatgcca ttttcaatag ctaaagcaca
     7561 aatgattgcc tctatcccac cggcagcacc cagtaagtga ccggtcattg atttggtaga
     7621 gctgacagca aggtcgtaag cgtgctcacc aaataccctt ttaatggcct gggtttctgt
     7681 cttatcgtta agttgagttg atgtaccatg ggcattaatg tagtcaacat cctccggcct
     7741 caaaccggca tccttaatgg ccatggacat gcacagctgt gctccggcac catcgggatc
     7801 aggagcagta atatggtagg cgtcactaga aagaccgtag ccggcaacct cagcatagat
     7861 tttcgccccg cgctttagag catgctctaa ttcttccaga ataacaattc cggcgccttc
     7921 tcccatcaca aaaccgtctc gttcactgtc aaaggggcgt gaagcacgct ggggatcatc
     7981 attacatgag gacatggcac gtaaagcaca aaaaccggca aaagctactg gtactaaagc
     8041 tgcttctgca ccaccggcca ccatcacatc tgcagcgcct aattgaatag tcttcattgc
     8101 ttctcctacc gaatgggtac ctgttgcaca ggctgtaaca atggtcgtgt taggcccttt
     8161 aagaccaaac tcaatagata tataggctga tgccatattt gcaattaaca tcggcacaaa
     8221 aaacgggctg actctacgtg gtcctttgtt aatcagaact tgtacctgtt cctggatagt
     8281 ggtaatacct ccaatcccag agccaacaga aacaccaacc cgttgattat cgatttcaga
     8341 tgattccagc ttagcgtctg ccatagctaa tctagctgct gcaacagcaa actgggaaaa
     8401 gcgatccata cgacgacttt cttttttgtc catataatcg gcaggattaa agtctttaac
     8461 ttcggctgcc acctgtgtcg gataatcatc tacattaaat ctttgtacac gatcaatgcc
     8521 cgattttcct gctaatagat tgtcccaaaa gattgtttta ccagtaccga cgggtgtaac
     8581 catccccaga cccgtaataa caacgcgtcg tttcaatctt ctcacctctt ctttccatgc
     8641 cttttctaaa gaagccctgc ctgcgcaggg cttcaggtgc tcacgcctta ggcattagcc
     8701 tcgatatagc gcacaacatc tcccactgat ttaatgtttt caacttcctc atcggagatt
     8761 tcaaggctaa attcttcttc aatggccatc actaactcca ctatatcaag ggagtctgca
     8821 tccagttcct cgaaagaagt ttccaaagca atgttagctt cgtctgcgcc caattgatca
     8881 cttattagtc ctttaacttt gctcaagaca tccattgtct cacctcctcc ccataagata
     8941 ctaaataaaa taatcattaa tgtcaactag cacatcacaa gtccaccatc aatggcaata
     9001 acttgccctg taatgtaggc agcctcatct gaggctaaaa acacgaccag cgcggccaca
     9061 tcttctggct caccagtacg gcctaaggga atttgtttga caatattgtc ttttacttca
     9121 ctactgagta ccgctgtcat agctgtatta atatagcctg gtgctactgc atttactgta
     9181 acctgacgtg cggccaactc acgtgccagg gactttgtca tccccatcag gccggcttta
     9241 gctgcagcat aattaacctg accaatacca ccgattttgc ccacgatgga cgccatatta
     9301 atgatgcgtc cacccgtctt ttgttttaaa aatgggcgga ttacagcttt agcacagtta
     9361 aaagcgccag tcagatttgt atctagtact gcttgccatt catcagcttt catccgggca
     9421 gcaatgttgt cacgggtaat gccagcatta tttaccaaaa tatcaatttt accgaaatgc
     9481 tcttgggccg ctttcactag tttattacaa tcagctgctg cggtaacatc agcttgtact
     9541 agtagtcctt gactacctgc ttcttgcaca gctgctagcg tatcctctgc ggcctgggca
     9601 ttggcagtat agttgatgac tagttttgct ccttcacgtg ctaaggcaat ggcaatggca
     9661 cgcccaatgc cgcgtgaggc accagtaaca atagctacct tattgactaa acgcaatatt
     9721 ctcaccctcc cctgcaccga gagcttctaa cactgctgcc agtgtcttgc gatcttccat
     9781 ctggtgcacc caaacgtcgg tattagtttt cttcattaag cccgtcaagc ttttacctgg
     9841 acctacttcc acaaaacggt taaacccatc cgcaattaat ttttccatgg aatcttgcca
     9901 taagacagag tgactaacct gacgcactaa agcatcacga atagcggcag tcgaattaac
     9961 ataagcggcg gtgacattgg taatgacggg gatttccacg ggtttaatct gcaccttgtc
    10021 taattctgct gctaataatg gttctacact ttgcaataag ctgcagtgaa aagaaacact
    10081 gaccggcaat tcgacagttc gtctggcccc atgctctttg gccagttcac aagcacgacg
    10141 gagggcagac agctcaccag cgatgacaat ttgcccggga gcattaaaat tagcaggctc
    10201 tacaatcccc acctcactag ctaactggca gactgttgca actttttccc gcgctaaacc
    10261 caagatcgcg gccatgccgc caactccggg aggtaccgcc tcctgcatca actgaccgcg
    10321 cttctgtacc aacggcaggg catcctgtaa ggacagcgca cctgctgcaa ctaaagctga
    10381 atattcacct aaacttaagc ctgctgctgc atccggcact aaaccgtgtt gctttagaac
    10441 tgctaaaaag gctaggctag ttgccacaat tgctggttgt gtgatttcag tttgttttaa
    10501 ttgttcatca gtaccatcaa agataatgtc cgataacttc atagaaaggg cttcatcagc
    10561 tgccgtaaaa acttctctcg ccacaggata atgttccgct aactcgcgtc ccatcccaat
    10621 gtactgcgcg ccctgccccg gaaagataaa tgctattttt cccatcttta ttcacctcgc
    10681 atctgactaa ccaataaact ttgccagttc gctgatgaca ctacgtgtct gttttattaa
    10741 gtcattaata atgtcttgtg ctgactcttc cttcgttacc atcgctgcac tttgtccggc
    10801 cataactgag ccattcacca catcgccacg acgtacagcg tccgacaaac gaccaaccgc
    10861 aaatttatct atttctgcca aactagcacc ttctttttct aggcggctaa tttcacgtgt
    10921 taaattattg cggataacgc gtacgggatg cccagtagac acacccgtaa ttaccgtgtc
    10981 tcgatctttt gccttcagaa tcataccctt atactgtgca tgcgccgtac attcatacgc
    11041 gctgataaag cgtgtgccca actgcacacc gtcagcccct aaagccaagg ctgctgcaac
    11101 tcccctgcca tctgctatcc caccagcggc aatgaccggt acattaacag catccactat
    11161 ttgcggtacc aacgccatgg tggttaactc cccaatatgg ccgcccgcct ccataccttc
    11221 agcaatcata gcatcaacac ctagtcgtaa taagcgtttg ctcaaagcaa cagaggatac
    11281 tacgggtatt actttcgcac cattttcctg aaaaagtttc acatacctac cgggattacc
    11341 tgctcctgta gttatcactc gtatcttttc ccggcaaagg agttctacta cttcctcaac
    11401 gtagggggaa agtaacataa tattaacgcc aaaaggctta tctgtacgcg cttttgcttt
    11461 cctaatttcc tgctccaata ctgcagcagg tgcattaccg gcaccaataa taccaagtgc
    11521 ccccgtctca gagacagctg cagccaactc accggttgcc acccaggcca tgccaccttg
    11581 taagatagga tactgtatgc caagtaagtc acatagcttc gactttaaaa ccatcatctt
    11641 cctcctgtca atatctaact aaggcagctc cccaagtcaa tccggcccca aagcctacca
    11701 gtgccatcag atcgccgcgt tttaaacgtc cttgttggat agcttcatct aaagcaactg
    11761 gaatagaagc agaagacata tttccatacc gatcaatatt gacaatgaca cgctctgggg
    11821 gtaattttaa ccgtttacgg gcagactcga ttatccttaa attggcttga tggggaacta
    11881 aaaaatctaa atcctctttt accaaacctg cggcatctag tgcttgttgt gtaacttctc
    11941 ccatcacccg tacggcaaat ttaaaagttt cgtgaccatt cattttaata taatgttgac
    12001 gctctgcaac actttgctgt gaagctggca gctgtgaacc acccgccggc acgacgagca
    12061 gctcggccag agagccgtcg gcacctaagg taacattaat aaatcctggc tcctccacag
    12121 gtcctaaaac tactgctccg gcaccatcac caaaaagaat acaggtgctg cggtcggacc
    12181 aatccaaaaa tttagacatc acttcggcac caatcaccaa aacccgccga taaagcccgc
    12241 tttggatata cagacgtcct accgaaagag cataaataaa accagtacag ccggcagaaa
    12301 gatcaaaagc aaaagccttt tttgccccaa gttttgcttg cacaaaacaa gctgttgaag
    12361 gcatgatggt atctggtgta attgtcgcca cgataattaa atctatttcc tcagcagtag
    12421 tattcgccgc ttgcagagcc ttttccgcag cttgtgcggc taaatcagat gtaaacacac
    12481 catcttcaac cacacggcgc tcactgatac ctgttcgcgt acggatccat tcgtcactgg
    12541 tatcaatgat tttttctaag tcgctattcg ttactatttt ggggggtaca gctgagccca
    12601 cccccaaaat ccctgcatcc tgtaatttca tacacagtac ttcctttctt tcaggcagga
    12661 cagctattag tcggttaaaa gtgcggctaa ttcctgttcg ataagtccgt ttacgttttg
    12721 gcgagcatac agatacgctt ggttaacaat ggcgctgcga atggcacggg catttgaaga
    12781 accgtgtcct ttaatacagg cgccgccaac acctaagaac ggtgctccac cgtactctgt
    12841 ataatctaac ttggctttca gcgctagtag ttctggtttc aatagtgagg cagctatttt
    12901 actttttaag ttagctgaca tagcttcttt taatgaaccg aacaatccgg caaccaagcc
    12961 ttccagggtt ttcaggataa tattccctga aaagccatcg caaattatca catctgccac
    13021 accatctaac acattacgtg cctcaacatt ccctataaaa cagggcagct tttctttcat
    13081 cagttgataa gcctttttaa cttgttcatt gcctttgatt tcttcaacac caacattcag
    13141 taaaccaaca cgcggttctt tttttaacaa aacttcccgt gcatatattt tacccattat
    13201 tccatattgc agcaactgtt cggcttttgc atccatattg gctcccacat ccagcagcac
    13261 tacattgccg cccgggaaag tgggtgctac tactgttaaa gccggccgct cgatcccctc
    13321 aatccggcca gttaataata agctgccggc catcagggcg ccagtattac ctgcagaaac
    13381 catggcctcc acttttttat ctcttaacat ttgcagtgca accatcattg acgaattctt
    13441 tttacggcgc acagccagga caggagaatc ttcattttta ataatttctt ctgctggcat
    13501 caatgctacg cggttttgat cataaacctt gtcgcgcaaa taattttcca gcacagcttg
    13561 tggacctaca aaatgtatgt gcagatcagc cagttcggtc aacgccaata ggccacctgc
    13621 tattatttgt ttaggggcat gatcccctcc cataacatcc agtgccactc ttatcactag
    13681 tttcacctct tgcaaaacat ttgccacctt caagcttttc cctcggcaag atttccccgg
    13741 ataacaaaaa cccttcctta tgcaggaaga gtatttgata tgttgattgt tattttattt
    13801 ctacagcttc tctgccttta taatagccac aatggacaca cacccggtga ggcaatttgg
    13861 gttcatgaca ttgcgggcaa gagattaaac cgggaacagt aagtttccag tgtgttctcc
    13921 gtttattttt tctggatttg gatgttttcc ttttaggtac cgccattatt acacctcctc
    13981 atactactta tccagcagtt tctttagaac agataagcgg ggatccatat tatctactgg
    14041 acaactacac tgtttttcat taagatccgt accacacgtg gggcagatgc cctggcatgc
    14101 tgcctgacaa agaggtttaa aaggcaatgt ggttataaat aattctctaa ccaactcaga
    14161 cagattaatt tccttttcct cacttaccgg gatctcatcc tcaaattcct cttgaaaaga
    14221 aattgggact tgttttaaac agcggctgca ggtaagaatc acccttgtgc gcaggcttcc
    14281 tctcacttct acttttctat cgcgatatgc tgcactcatt tggacctcta ggggagcaat
    14341 aacttgcaaa tcctcctcgt ccaaattaag tgctgactct gccacagtaa aattataagt
    14401 aatatcttta ccaggcgcat cttttaaagc ttgtagctca attttcatag caaaaattag
    14461 cacctcgctt gcgctaaatt ttattatata gatgaataac tttattgtca acatttaacg
    14521 ggacagccgt gtaacggaat tctacttctt attacgccgc tttactgcct tttttatgca
    14581 caggtaaaca attacttgcg cttttttcag ctggcgttaa ctcctgcaaa aaatgtaagg
    14641 cttctgttaa tgtttgtacc ggtaccaagg taattttttg ggctgcctgc ttagcatctt
    14701 cgtaattctc aagcggcaca aagaagtatg cggcaccagc cttctctgct gctttaacct
    14761 tttggtgcac cccgccgatg gcaccgacct cttccagcaa attaattgta ccggtgcccg
    14821 ctattttatg tcctgctgtt aaatcacgcg cctcaagctg ccgcataatt tcaagcacaa
    14881 acattaagcc agctgacggt ccggaaatct cgccggcatc aatctctatc tctagcggca
    14941 aaacaggctg ccagttaagc gtctgaatca acacacggat agcggcccgc tctggtagtt
    15001 ctgtatggtt ggtagtagcc acctctacct cacgttgttc accattacgt ttcactgtta
    15061 aggaaacggg gtcaccaaca ggtcgcagct gcacctgtga aataagttct tctgccaagt
    15121 agacagaatg accgtctact gccacaatca catcttcggg aaagagtaat ccttgtgctg
    15181 ggctgtcctc ttccacatca actacttcta caccatcact atggataggc acctcataac
    15241 ctgagtgacg taacgcaatt actttagcca agttctggct ttctgtcatc caacgctgca
    15301 tcagttcgtt atactcctga cgatccatat tagggggaat cacactggta ctgggttgta
    15361 attctacatc gggatgcagc aacgcataac acagcaagac aggtgttgct cgctgctggg
    15421 aaacagtaac tagataaaat gtcccgttat catcatccgt actgccttgc actgtgacaa
    15481 aacgcgacaa gtcttctgcc gagccaggct tgatcaacaa atacccagtc ttggcaaaaa
    15541 gtaaaagcgc acccacaacg ataatggcaa taattttacg taattgtttc atttagtttc
    15601 cacaaacctt ctttctctag catttgcctg atgctaggta acgcctcgtg cgccgcttct
    15661 tcacctgctt tgatggcttc tttagctcga tgaaaatgtc ctggtgctat ctcccgcagg
    15721 gcagggctga tcaccacatc ggctgcctca aggctataac gagataaatc ccgctgcatc
    15781 acatctagac tttgtgttat tacatctaaa atatgttgtg gtttatgacc atcaagatag
    15841 atccccacat ctacagcaat cacaagatcg gcaccaaggg tccgcgcggt gggtgcaggc
    15901 acacgctcaa tgacagctcc atcaaccaat acttgctctc cctcttcgag cgggcggaaa
    15961 atccccggaa ttgctgctga agcacgtaca gctcgagcca ctaagccctc ctgcatcaca
    16021 actttctccc cacggcacaa atccgtggcg acgatagcta aaggtacctc aagttggtca
    16081 aacgtttttc tgcgagtcag cagtaacatc agctgctcta gtttatcccc ggcggccaaa
    16141 cccatatgcg ggaaagtcca atctagccat aacttactat ccgcatggct aaataaacgt
    16201 tctagcaggg gcaattccaa accggcacaa taagcagcac cgactactga ccccatactg
    16261 gttcctgtta ttaagtctat gggcacaccg gccaacttta acactttaat gacaccaata
    16321 tgagcaaatc ctcgtgccga accgccgcct aaagccaagc cgactttttt tcctctttta
    16381 aacatcgcat ccacctcaat ccggcatact ccggcgaatt caaatatata tattactatg
    16441 ccaataataa ttataccgcg ggagggaata tgaatacaaa acgctttaac agggatcgcg
    16501 tgcccgccta tttgctgggc atcgctgcgg tcgcgctggc cgttgctctg gtcctgcacc
    16561 cgagcgaagc ctttgatgct gccgttaagg gattagaagt ctggtggaac gtagttttcc
    16621 cggcgctgct accgtttttc ataggttcgg aaatattgat ggggcttggg gtcgtccatt
    16681 ttttaggagt gctgttagaa ccattgatgc gtcctgtctt taatgtgcca ggcgtaggtt
    16741 cctttgttat tgccatgggt ttagcttccg gcttcctcgt tggtggcaat cttagcgccc
    16801 agatgcgccg caaaagcttg attagtaaag tagaagcaga gcggttagtc agctttacca
    16861 atacatctga ccccctcttt atgtgtggcg ctgttgcttt gggtatgcta ggctatccgg
    16921 agataggtgg taccattgtt gctgcgcact atttatccgc tcttaccaca ggcttgttcc
    16981 tgcgctttta tggcagaaac cagcccaata cacctgaaac ctttgagcgt gggcgtatgc
    17041 tgtcacgggc agctacagct ctctatcacg caagaaaaaa agatggccgc gccttcggcc
    17101 aactcatggg cgatgctgtt cgcaattctg tcaacaattt gctgctaatt gggggcacta
    17161 ttatcctttt ttccgtgatt atccgcatcc tgacagtcac tggggttgtt ggtattatta
    17221 gtgctgccgc ctacaaaata ttgtttggtc ccttaggcat gcatcccgat ttgatgccag
    17281 cagtagtcaa tggatttttt gaaattgatc ttggttgcct ggttgccagt caagtaagta
    17341 ctgccgtacc catgatacaa aagattatgg tgacaagcgg cattattgcc tggagtggcc
    17401 tctcagtctt tgcccaaatt gccgccatca ccagcgatac agatatgaaa atgaagccat
    17461 ttgtgattgg ccgcatgatc caagcctttt ttgctgtcct ctatacctgg ctttttatgg
    17521 gaccattagc gccggtaatg tctcgagcaa tgccggtctt ttcccatctc cacccgcctg
    17581 ttggtatgac agtgctgcag agtttaggtt atatgagttg gctttttatt atcctggcgg
    17641 ctggattgtg cctgattggc ctgctgctca atatttttaa gggcattaaa atttctgtct
    17701 ttagacggta actacttttt tcatcaccaa ctcataaatt tctggaggca caaattcaga
    17761 gatatcaccg ccaaagatac ctacttcttt gaccacacta ctggaaatat aggaataacg
    17821 gtttgttgta gccataaata gcgtctctaa ttccgggtct aattgacgat tggtaattgc
    17881 catttgaaac tcaaagtcaa aatcccccat cggtcgcagg cctttaagca taacctttgt
    17941 ctgcttcatt tttgcataat caaccaataa accttgaaag gaatctacct cgacattagg
    18001 atagttttta actactagct ttaacatttc tactcgctct tctatggaaa acatcggtgc
    18061 tttgcgggga ttatctaaga cggtgacaat aaccttggag aaaacacgac tggcacggtc
    18121 aataatgtca cggtggccat tagttaccgg atcaaaactt cctggatata ttacgacaat
    18181 caatttgcgc gcctcctttt atcactcaag ctttgcttgg cgtcagaaaa gtaagtatag
    18241 tatcaccata ttttttaaca cgatttgttt gccattcttc cggctggtac cagtcactat
    18301 gctgacgatg atgctcaaca acaacaattc cggctggtgc caataaatca ttagcagcga
    18361 gctgtctgag aactgaagcc aagaccggac tatgatacgg gggatcaaga aagattatat
    18421 cgaattgctg ctgttttatt ttcagctgtg ctaaggctgt taaagcatct aactgcagaa
    18481 cttcaccccc tgcctgcaaa ccacataaaa acaaattccc tgttattatt ttagcacaat
    18541 gggcattttt ttcaataaaa acacattttt ccgcaccacg acttaaggcc tcgaggccga
    18601 taccaccgtt accggcaaag atatcaagaa aatacgcgcc ctgtagccga ttgctaagga
    18661 tattaaagca tgattctttc actctatcgg ctgtagggcg cgttgggctg ccctttctag
    18721 ttttgagctg tctgccacgt gcacagccgg caataatccg caaagctatc acctcctggt
    18781 aaaattacta catcaaaaaa tgccaagtcc catttttttc ctagcatttt gatgtatatt
    18841 cttattaagc actgttcata ctaggagtac aacatattcc ccataagctc ttcctccggt
    18901 ttctcctctc c
//
LOCUS       DASRMS010000132         1144 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_505472, whole genome shotgun sequence.
ACCESSION   DASRMS010000132 DASRMS010000000
VERSION     DASRMS010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1144)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1144)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1144
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505472"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            244..549
                     /locus_tag="VFC74_06110"
     CDS             244..549
                     /locus_tag="VFC74_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24944.1"
                     /translation="MKNLLFGLALSVAVSAPAFAGEQMSKEQFCAELETEAVAIMSAR
                     QSGVPISKVLALVDNEGIHKHIVMDAYDQHAMQTPENAANQRAEFGVKWAVFCIKNR"
     gene            629..>1144
                     /locus_tag="VFC74_06115"
     CDS             629..>1144
                     /locus_tag="VFC74_06115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24945.1"
                     /translation="MQEELLGASEYEAWLKKFETKKTTDSCYTPAAVYEAVADWAVNE
                     YGLQGRRILRPFHPEGDYEKLEYQAGDVVIDNPPFSILTKIVRFYESKGVDFFLFAPT
                     LTLFSPNAKCSIVTNSTIEYANGAKINTSFITSLDDAKVRTAPELKKAIADAEAAIRA
                     EKPKLPKYIYPS"
BASE COUNT          352 a          223 c          294 g          275 t
ORIGIN      
        1 gaatggagca ggagccataa cccaaagatc actgcgctaa tgaagcgcca gcggatctgg
       61 tggatagtga tctatgaatg acgaaaacca agacagcata agaaataccc tcactgctgc
      121 gctcaacacg atctgcgagg atcacggagt ttgcattacc gcgctaactg cagaatggat
      181 cgatgtaagc acatgcggcg aaagtaaaaa gctactaaca tgtttagaaa ttgaggcgtc
      241 attatgaaaa atctattatt tggcttggct ttatcggtag cagtgagtgc gccagcgttt
      301 gcaggtgagc agatgagtaa agaacagttt tgcgctgagt tagaaaccga agctgtagcg
      361 ataatgagcg ctaggcagtc tggagtaccg attagtaagg tactggcgct tgtcgataat
      421 gaaggtatac acaagcatat tgtcatggat gcttacgatc agcacgctat gcagacacca
      481 gaaaacgctg caaatcagcg cgcagagttt ggtgttaagt gggcagtctt ctgtattaaa
      541 aacagatagc cgtattgtgg tataatcaac ccgtcgtaat ggcgggtttt aatggtggga
      601 gttatgacag attgggggtg cgcaataaat gcaagaagaa ttgttgggcg caagtgagta
      661 cgaggcttgg ttaaaaaagt tcgagaccaa aaaaacaact gatagctgtt acacgcctgc
      721 tgctgtttat gaagctgtgg cagactgggc agtgaatgag tacggattgc aaggccgccg
      781 gattttgcgc ccgtttcacc ccgagggcga ctatgaaaaa ctggagtacc aagcgggcga
      841 tgttgttatt gataacccgc ctttttcgat actaaccaaa atcgttaggt tttacgaaag
      901 taaaggagta gatttttttc tgtttgcgcc aacgctcacg ctgtttagcc ctaacgctaa
      961 atgctcgata gtcacaaact caactattga atatgccaat ggtgcaaaaa ttaacacgtc
     1021 ttttattacg agcttggatg atgcaaaagt acgcactgca ccagaattaa aaaaagcgat
     1081 agcagacgca gaggcggcga ttagagcaga aaagccaaag ctacctaaat atatttaccc
     1141 tagc
//
LOCUS       DASRMS010000133        17770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_505746, whole genome shotgun sequence.
ACCESSION   DASRMS010000133 DASRMS010000000
VERSION     DASRMS010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 17770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17770
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505746"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..125)
                     /locus_tag="VFC74_06120"
     CDS             complement(<1..125)
                     /locus_tag="VFC74_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003509988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZK24946.1"
                     /translation="MTIPLGDFLQQLVSNPALLVIATLTAAVILVNGWTDAPNAIA"
     gene            complement(351..521)
                     /locus_tag="VFC74_06125"
     CDS             complement(351..521)
                     /locus_tag="VFC74_06125"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HZK24947.1"
                     /translation="MQIVNMEIETERKLLGKLMITESIDSRAILKQSEKLDMLINLYN
                     RLERFIFENACP"
     gene            complement(665..1111)
                     /locus_tag="VFC74_06130"
     CDS             complement(665..1111)
                     /locus_tag="VFC74_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="demethoxyubiquinone hydroxylase family protein"
                     /protein_id="HZK24948.1"
                     /translation="MVKKINKDELLARLKEFYLLEKFQVQYYQAQLSAANDAYFQCAF
                     TKMVQIESGHADYFAKKLIEAKVELPQARTSLLQLAGRLLGESVELTGAKQTCKLGIA
                     LESKAIEMYQKFILEAWDEQKLRNTLMEYLLDEEFHTLWMKDYLHR"
     gene            1770..2732
                     /locus_tag="VFC74_06135"
     CDS             1770..2732
                     /locus_tag="VFC74_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24949.1"
                     /translation="MKNLLAIKNLNVGFSNGVGAVRVIRDLSLNLAYNEALGIVGESG
                     SGKSVTSLAAMRLLPSVGTEVQGEILFDGKDLLSLSEPEMQAIRGNRMAMIFQEPMTS
                     LNPIHTCGRQISEAILLHSKATKKEAAAKALELLALCGIPDAKQRLKEYPHQLSGGMR
                     QRVMIAIALACDPELLIADEPTTALDVTIQAQILKLLKDIKHKKEMSIILITHDLGIV
                     SAFCDRVIVMYTGEVVEIAPVRQLFKEPLHPYTQGLLKALPKLTDTSRLEAIKGMVPD
                     ADQMPQGCHFHPRCPQASERCRSEKPPLVTLDDGRGVRCFLFDY"
     gene            2745..3728
                     /locus_tag="VFC74_06140"
     CDS             2745..3728
                     /locus_tag="VFC74_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24950.1"
                     /translation="MGAEVLVKAEGVKQYFTVKQERGKKATLYAVDEVDLTIYQGETF
                     GLVGESGCGKSTLGKMFLRLYPLTAGKIYFSDVDISKLKGQQLKDFRCQTQMIFQDPS
                     SCLNPRRKIIDILTEPYIIHKLYSKEERRHKVGELCEMVGLSQSYLHRYPHEMSGGQK
                     QRVGIARALALQPQLIICDEPVSALDVSIQAQVINLLQDLQKQFKLTYLFISHNLSVV
                     KHICQRIGVMYLGKIVELAPAEKIYQAAKHPYTQALIAAIPSLDDPDKVTKAEGQVLS
                     GDLPSPTNPPQGCAFHTRCPVATAICQLEKPPLLEVEPEHFVACHLCPARM"
     gene            3786..5399
                     /locus_tag="VFC74_06145"
     CDS             3786..5399
                     /locus_tag="VFC74_06145"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK24951.1"
                     /translation="MKRIVGILLLCAFVVAGFTGCSKGSSGATPGEGETEQKVIVIGA
                     QSDLITLDPGHMYEPYANMISYAAYDMLYRVQPGTMGTPTPSVATGVNIDETDTVYTF
                     TLRDDVVFASGNQLTANDVVWSINRVLNMKDSNAYSNVKIVEKVEAPDDKTVVITLTE
                     PDASFLVKLTSNAYSILDSAVVKEHGGSDSGNDTAKAWLDTASAGSGPFIIKSWTPKE
                     QLVLEKNANYWGEAGNIDSIILREMPSVDAQITALQNGEIDIALGLNSETAKQLAGKE
                     GVTIANGSTALMTFLVMSRDETMSKEMSNPLVQEAVRFALDYEGYLTLGGEGCTIPLN
                     FVQQGFAGALTRKADSRDLEEAQRLMAEAGYADGFEITLTCANNNSEGLEWTTIAQKV
                     KQDLAEIKINVKIETLETTLVYEKMRDAAMPFYVMFWSPDYYDINNQFAFLPGTGDSG
                     TAYGNRTKWERTAANEKMWELANKIQVETDTTQRAQYAEELQEFFALDNPFAFLLQHA
                     KTFAYNSNTLQHVSYNDLCKIELCELVAK"
     gene            5445..6458
                     /locus_tag="VFC74_06150"
     CDS             5445..6458
                     /locus_tag="VFC74_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24952.1"
                     /translation="MVRFLLRRLALTIVLLLGVTVMVFLISHLVPSNPVAANLSQTAL
                     ADPEIVAAYEAKWGLDKPLSEQYFIYITNIFKGDFGTSIRTGRPILQDLKQYFPATLE
                     LSLLAMLIAIVFGISFGIISAVWQNKPIDQLLRAISVSGVSLPSFWFALLIMSVFSGT
                     LKWFPSGGRINPRLFNPTGGTGLYLLDALSWGDWGMLSDSLAHLILPALVLGFFTMGL
                     ITRQTRSNLLETMSMDYIRTARAKGMRESRVVCAHALNNALIPVITVSGMGFCNLLGG
                     MVFVEKIFSWPGIGQYAYLSAITLDFPAICGVSLLIAATYVVINLLIDILYGIIDPRI
                     RYQ"
     gene            6471..7313
                     /locus_tag="VFC74_06155"
     CDS             6471..7313
                     /locus_tag="VFC74_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002585722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24953.1"
                     /translation="MMNAIKQLLKQKNFMFTLGLIICVAWILVALFASLLSPYDPVKD
                     QNLAIRFKPPSSAHYFGTDSLGRDVFSRVLYGSRISLAAGLVTVLISFFVGLLFGGIA
                     GYVGGIVDDFMMRFSEMVQSFPPLILAMVIAAALGPNITNSIIAMAIIWWPNYARLTR
                     SIVISVKENDYITASRVLGATHLRILYKEILPNSLGSLIVMCTLDLGNAILMFSGLSF
                     LGLGVQPPVAEWGAMVSDGVLTFNYWWISTFPGLAIFSVAMGANFIGDGLRDYLDPRM
                     RRQI"
     gene            7345..7686
                     /locus_tag="VFC74_06160"
     CDS             7345..7686
                     /locus_tag="VFC74_06160"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK24954.1"
                     /translation="MKKVTLDSVPITGLPLYEMQQLFQINEPCSEIKISYITLLPGQR
                     VPESGTGVHEEDEYSYFIEGEVYTESGDYQGVCGKGDATLIPQGEAHWSENLTDKPCR
                     IVCVLVKTQKK"
     gene            7700..8770
                     /locus_tag="VFC74_06165"
     CDS             7700..8770
                     /locus_tag="VFC74_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007493692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HZK24955.1"
                     /translation="MNKQRIERVIATMQTAGLQQMIVSATAALYYLTGYWVEPHERMI
                     ALYLDAAGQAVLFGNEIFGLPSTPALPLVCHKDSDNPVADLAKHAAPGKLGIDKFWYS
                     KFLVELLELRPDIVPVHGSAPIDLARTTKDEAEIAQMRHSSLINDQVIAAVIGMLQEG
                     ITENQLASQVNQQFLQHGADSEGPQLVCFGANGADPHHSGDQTKLQPGDSVTFDIFTP
                     INRYWCDMTRTVFYQTVSDRQRQVYELVKQANEAAIAAIKPGVLLSTIDQTAREIIIQ
                     GGFAPYFTHRLGHGIGLECHEPPDVSLASEIPLTPGMIFSVEPGIYLPDEFGVRIEDL
                     VLVTAAGCEVLNHYSKELQVIK"
     gene            8948..9799
                     /locus_tag="VFC74_06170"
     CDS             8948..9799
                     /locus_tag="VFC74_06170"
                     /inference="COORDINATES: protein motif:HMM:NF014600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HZK24956.1"
                     /translation="MKRQQRASSRKAPRRNRSLTLLITLLAVVILTGYCYRDTLYAYW
                     DADVPPVDAPVEEPPSGTPADTEPPAAEEPPPVEKTSVYPPAPGEILQLANGNDLLAL
                     VTKETSLGQYKPTDLVTVPAEMRLYDVEYQLRAEAYQQLRAMWEDAGAAGVDFFVNSA
                     YRSYATQKRLFNNYAAKHGEAEANKFSARPGQSEHQLGTTVDVGIAGHVLTNSFGGTT
                     PGRWLVENAHRYGFVLSYPKNSSEITGYIYEPWHFRYIGVDMAAAWRESGQILCQYLA
                     QQPQQWQ"
     gene            complement(9886..10869)
                     /locus_tag="VFC74_06175"
     CDS             complement(9886..10869)
                     /locus_tag="VFC74_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010077562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HZK24957.1"
                     /translation="MTQLDTPLVSCKLHLHNRLVMPPMATAKADDEGKITKGILEYYQ
                     DKTQGGYISLVIIEHSYVAVAGKASKRQMSVSSDRDIPGLEQLASAIHANEAKAAVQI
                     NHAGSTGIADEVMGPSAVAHPNKQRIPEALTVGQIEKVIAQFQAAAGRVQKAGFDAVE
                     IHAAHGFLLNQFYSPLTNHRTDEYGGDLHGRIRLHLDIIKAVREAVGESFPLMLRLGG
                     CDYQDGGSTIEDSVIAAQAFAEAGVDLLDISGGIFGYMKSTSREPGYFADVSSAIKQA
                     VDLPVILTGGITSRQEADDLLARGAADLIGVGRAIMQDSLWAQKALAGTVN"
     gene            11014..11481
                     /locus_tag="VFC74_06180"
     CDS             11014..11481
                     /locus_tag="VFC74_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24958.1"
                     /translation="MIPIPEFYIYPIYTGILALTLIALLPREEIKRLVIYGIVFGAVT
                     DVAIIGVISNLLKVGGHISYYPFGALGVAFFPPLAWGIWFILFFYFLPTDLPWVVIYV
                     FAAVGTSVLFSNVLVNMQIFVWNYGRILVPFLIYGLWFSFAAWAYKRLTKHHA"
     gene            11630..12391
                     /locus_tag="VFC74_06185"
     CDS             11630..12391
                     /locus_tag="VFC74_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NRDE family protein"
                     /protein_id="HZK24959.1"
                     /translation="MCLVVFAYQQHPNYKLILAANRDEFYNRPTARATFWADCPDLLA
                     GRDLQAGGTWLGVTKSGRFAALTNYREPAWEVTNRRSRGDVVRNYLCNQITPAEYAEL
                     LRQTGTEYNVFNVIFGDVNSLWYYSNVTGEPLLLTPGLYGLCNHLIDTPWPKVARSKS
                     QLAQSLNSTPLNKDQLLALLKNKKPVPDHELPDTGVGLEWERILSPVFVESETYGTRC
                     STILTIDEAGSLDFLERTYITHSPVYEDVSFSFKV"
     gene            complement(12397..12735)
                     /locus_tag="VFC74_06190"
     CDS             complement(12397..12735)
                     /locus_tag="VFC74_06190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24960.1"
                     /translation="MPKAKSEIPFSNNNRFYLELMLVLVIIILIIALAAPFYNATLQL
                     VRDRVDEANIRILNSATLQWLLADEANDPQKMDTLELQAELINDYLLEWPISPNSSTY
                     QLEDGQWIAK"
     gene            12941..13363
                     /locus_tag="VFC74_06195"
     CDS             12941..13363
                     /locus_tag="VFC74_06195"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZK24961.1"
                     /translation="MGKFLKKLFQKQQGQAITEMALTLPILLLILLGIIQFGFIFNGQ
                     IAVASAAREGARLAAVGAQDAEITATVQSTLAGIALLDNQSTNVTPSGTRIFGQQVTV
                     EVAASTNIVVPFFNLVLGENFNHTSSATMRVEFVQGGS"
     gene            13365..14297
                     /locus_tag="VFC74_06200"
     CDS             13365..14297
                     /locus_tag="VFC74_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZK24962.1"
                     /translation="MKFLQRLLREEKGSAMVLVTIAMVILLGSSALVIDYGQLVLIRR
                     KLVNAADAAALAGAQELLSEPASPSNAMSTAEAYAIANGVDVDQVTAEVTHDKSQITV
                     TVTGIVDYTFARVLGFEQGNVSAEAKAAVGSIQGMTGVAPLTIVEQSFVKGQSYELKS
                     ASPSEMGPGNFGVLALGGSGASRYEANLRDGYSGNIKIGDLLDTEPGNMSNPTKRACT
                     DRINACNLGCTYQNFKPDCPLLVYIPVIKQPTGQGRQEVEVVGFAAFFLDKHQPPGQG
                     NESSIKGWFVDTIVPGQINPGETGYGVSAVNLIK"
     gene            complement(14374..14889)
                     /locus_tag="VFC74_06205"
     CDS             complement(14374..14889)
                     /locus_tag="VFC74_06205"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZK24963.1"
                     /translation="MCKDSLNRRGLSLVELIITMTILLIVLAVGYPFYHFGTRTASVG
                     EDQSNIQRDIRLNGDFITKELRNATEIEIVPSASEGSGDTDNQYFYLENNNIMHKTNA
                     NPTNPSTFTNRNILSLTFTLQQTAAGNNFVTFIVSGQEGTQTYSVESQVHLNNLTGLP
                     AATGTVIRYKR"
     gene            complement(14902..15603)
                     /locus_tag="VFC74_06210"
     CDS             complement(14902..15603)
                     /locus_tag="VFC74_06210"
                     /inference="COORDINATES: protein motif:HMM:NF015140.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007154 - cell communication [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Calx-beta domain-containing protein"
                     /protein_id="HZK24964.1"
                     /translation="MKLLTLNKRGMTLVEVLVALVIIVIMIAALVPLFSSSYGGIFVA
                     GRRSSAKFYVQEEIEKAIAGDSGTTANIAPVSDIEGVAGAIVELEHEYEDGRTVVIKT
                     FYREQPVWLQFALEEYKAFKDDDLIEIKVTRKGITDSTATISYKTVDDFSSAIPGTHY
                     KATSGTLTFAPGETVKAFPVEVINYDTDPNNTETASFTVKLSLSNPATSGGYSALTGF
                     PSSAVLHIIEHIPES"
     gene            complement(15603..16700)
                     /locus_tag="VFC74_06215"
     CDS             complement(15603..16700)
                     /locus_tag="VFC74_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24965.1"
                     /translation="MLKNNRGAAMPIALLVLFVLTLLGTVLWQYFMTDTMQVARAEEM
                     TQAYYLARSGVEVGLGNLENVLYNSVSTITSIDELETKLTYIPSISAVDTGTFDVNYE
                     VDKTNSSVKIISNGTAAEVTQKEAAIVNFHFPALNLNPPQWLNNGYIVNTNDKNPSDQ
                     TGNIAQVSNKENVGHAIKHTSGLTDLYASFIAFIDAAPSLEIGNNATLHLHAGIVSFY
                     DSIYLSDHTKSLLYLHPNLKDSAKHRFIVNSSASAGPHTYSTSIRIKFSGSDFTIDGV
                     QTNTFVSNEYVGIVYFGGDVKESHGNSWNTILNVNNKYYFFKDGTAIHNGENQINNNN
                     LIRINNPDVLEEVFKMAEALPTVTSVTWGDR"
     gene            complement(16773..17435)
                     /locus_tag="VFC74_06220"
     CDS             complement(16773..17435)
                     /locus_tag="VFC74_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24966.1"
                     /translation="MYENDRGAALPLILVIMLVLGLLGATLIQYSVADAAQVAKEANK
                     LEAHYAARAGADAMADFLIKNPGKIADVAAKTNSSTYATGNVGDATFRTDVNETEDII
                     EIQSIGTANGVEQAVTLILSKDDNPNLFSNAIAQTSKDKHTLLLHEVDIKDEDGGDNV
                     SVEATGEILPTKENQNDNFRGTQNSNSSKYYPPPVFPKEGVDVSLTTFKDIVFILVVR
                     YN"
BASE COUNT         4864 a         4020 c         3875 g         5011 t
ORIGIN      
        1 gcaatggcat ttggtgcatc tgtccagcca tttaccaaga taactgctgc tgtcagggtg
       61 gcgataacca ataaagccgg attagagacc aattgctgca gaaaatctcc cagtggaata
      121 gtcatatttt cctccaagtt tcttgtaatc tagtcaactc ggtattcttt aagatctatc
      181 tataacttat tccgcagtta tcagcgtatt tcctttgttt taggccgaac aaggtaaaag
      241 tttcacatgc catcttatta gtctgacaca agcttcccat ttccaggcta gcacgctagt
      301 acaaacgaaa accagcctgc agctgcaggc tggttgggga gtgcaagaac ttagggacaa
      361 gcattctcga aaataaatct ttctaaacga ttataaagat ttattagcat gtctagtttc
      421 tcactttgtt ttaatatggc ccgactatca atactttccg ttatcatcaa cttgcctaat
      481 aattttcttt ccgtttcaat ctccatgttc acgatttgca ttattttgtc tttaaacatc
      541 gcgctcgcct ctttattatt tgtgtcgtag ctagattata tatttatttt ctgcccaggc
      601 tattaatagt ttcttatatt tgactctacg aatctggctt acatttttgc ttagcaagaa
      661 gaggttagcg gtgcaaatag tctttcatcc acagggtatg gaattcttca tccaacagat
      721 attccatcag tgtattgcgc agtttctgct cgtcccatgc ttctaaaata aacttctgat
      781 acatttctat tgctttactt tccaaagcaa tcccaagttt acaagtttgc ttggccccgg
      841 ttagctctac ggactcacca agcaggcgtc ccgccagttg aagcaaagat gtcctagcct
      901 gaggcagctc aactttagcc tcaattagtt ttttggcaaa ataatcggca tgcccacttt
      961 caatctgcac catcttggta aaagcacatt ggaaataggc atcgtttgca gcagagagct
     1021 gagcttgata atactgcact tgaaattttt ccagcagata aaactcctta agcctggcaa
     1081 gcaactcatc tttgttgatt ttcttcacca cctacagtat gtcgatcctc taatttacaa
     1141 atagttttag ctatagtatt attagcatac gcaaactctg cgattcccgc gcagaagtag
     1201 tcaagttaaa aataaccatg ttataaggag tagactacat gaaagcaaag ttaacagcac
     1261 tggcttcaat actaacctct tttatggcaa ctgcctgttg aatgggcccc ttatttttag
     1321 tgccgctagg tcttggcggc atgagtggtt tgtttgccgg caccctaact agatacagaa
     1381 gcctttttgt gattttagca gtgctgatgc tctacttgtc gcatcatttg gcagcgcaaa
     1441 ggcagcaacg gtggagcgaa aaactgatcc tgtgggcagc tacgctgtct accgtactta
     1501 ttttaatgta ccctttgatc caaaatagga ttctctagtt gtaaacggtc ttcgtttggc
     1561 ggattgttta aaactagcag aatactaaaa atgaggagaa atatgcaaaa gttatcttga
     1621 aaatcaagca ataatgtagt agcattaact aatacaacaa gagttgcggc gcctgacact
     1681 gtcactcgca gctcttattt ttaaactttc tcacttcact gcgttaaagt agtaacaaca
     1741 gctagcagtt tgtgcctgga ggcgtacttg tgaaaaatct attggcaata aaaaacctca
     1801 atgtaggttt tagtaatggc gtgggggccg tgagagtaat cagagatctc tccttaaacc
     1861 ttgcttataa tgaagcttta gggattgttg gcgaatcagg cagcggtaaa agtgtcactt
     1921 ctttggcggc aatgcgtctt ttgccgtcag ttggtacaga agtgcagggt gaaatccttt
     1981 ttgacggcaa agacttgctc tccttaagtg agcctgaaat gcaagcgatc agaggcaata
     2041 ggatggccat gatatttcag gagcccatga cttcgctcaa tcccattcat acctgtggcc
     2101 ggcaaatcag cgaagcgatt ctgctccaca gcaaggcgac taaaaaggaa gcagctgcca
     2161 aagccttaga gctattggcg ctgtgtggca ttcccgatgc caaacaaaga ctaaaagaat
     2221 atccgcatca gctttccggc ggcatgcgac agcgagtgat gattgcgatt gctttagctt
     2281 gtgacccgga acttttaatt gccgatgagc caacgacagc tcttgatgtc accattcagg
     2341 cgcaaatttt aaagctgctg aaggatatta aacataaaaa agagatgagc attattctca
     2401 ttacgcatga cttgggtatt gtctctgctt tttgcgaccg tgtgattgtc atgtatacgg
     2461 gagaagtggt agagatagca cccgtgcgac aattatttaa agagccgctg catccttata
     2521 cgcaaggcct gctgaaggca ctgcctaaac tcacagacac cagcaggctt gaagctatca
     2581 agggcatggt ccctgatgcg gaccaaatgc cgcaaggctg ccactttcat ccccgctgtc
     2641 cacaggcaag tgaacgctgc cgcagcgaga aaccgccctt agtcacttta gacgatggta
     2701 gaggtgtcag atgtttctta ttcgactatt aaggagtaga acatatggga gctgaagttt
     2761 tagttaaggc ggagggcgtt aaacagtact tcacagttaa acaggagagg ggcaaaaaag
     2821 ccacccttta tgctgtcgat gaggtcgatc ttactattta tcagggcgag acattcggtt
     2881 tagtggggga atccggttgt ggcaaatcca ccttaggcaa gatgtttttg cgcctttatc
     2941 ctctaacggc aggtaaaatc tatttttccg atgtggacat cagcaagctc aaaggccagc
     3001 agctcaaaga cttccgctgt caaacccaga tgattttcca ggatccctct tcctgcctca
     3061 acccccgacg caagattatt gatattttaa ctgaaccata tattatacat aaactctata
     3121 gcaaagaaga aagacgtcac aaagtagggg agctttgtga gatggtcgga ctctctcaaa
     3181 gctatctgca ccgctatccc cacgaaatgt ccggggggca gaaacagcgt gtcggtattg
     3241 cccgtgccct agcactgcag ccgcagctga ttatttgtga tgaaccggtt tctgctttag
     3301 atgtctcaat ccaagcgcaa gtaattaatc tcctacagga tctacaaaag cagtttaagc
     3361 taacctatct gtttatttca cataacctca gcgtcgtgaa acacatttgt cagcgtatcg
     3421 gcgtcatgta tttaggcaag attgtggaat tggcaccagc ggaaaagatc tatcaagcag
     3481 ccaagcatcc ctacacccag gcactaattg ctgctattcc ctcacttgat gacccagaca
     3541 aggtgactaa agctgaagga caagtactct caggcgactt acccagcccg actaatcccc
     3601 cgcaaggctg tgcttttcac accagatgcc ccgtggccac agcaatctgt caactggaaa
     3661 aaccgccgct gctagaggtg gagccagagc attttgtggc ttgtcatctt tgtcctgcgc
     3721 gaatgtaaac ggcaactcaa agagcgatct tttatataaa gaaaaaaatg ataggaggaa
     3781 gaaaaatgaa aagaatcgtg ggaatattgc ttttatgtgc cttcgtggtg gcggggttta
     3841 ccggctgcag caagggcagc agcggtgcga cccctggcga aggagagact gaacaaaagg
     3901 tgatcgtgat tggtgcccag tcagatctaa ttacccttga tcccggtcat atgtacgagc
     3961 cttacgccaa tatgatttct tatgctgcgt atgacatgct ttaccgggta cagccaggta
     4021 ccatgggtac acctaccccg tcggtagcca caggtgtgaa cattgatgaa actgacaccg
     4081 tctatacctt tactctgcgt gatgatgttg tttttgccag cggtaatcag ctgacagcta
     4141 atgatgtggt ttggagcatt aatcgtgtct taaatatgaa ggacagcaat gcctactcta
     4201 acgtgaaaat cgtcgagaag gtagaagccc cggatgataa aactgtcgtc attactttga
     4261 cggaacctga tgcttcattt ttagtaaaac taacttccaa cgcttacagc attctagata
     4321 gcgccgtagt caaagagcac ggcggctcgg acagcggtaa cgatacggct aaagcttggc
     4381 ttgataccgc ctctgcaggc tctggcccct ttataattaa aagttggacc ccgaaggaac
     4441 aattggtctt agaaaagaat gctaactact ggggcgaagc gggcaacatt gattccatca
     4501 tcttaagaga aatgcccagt gttgatgcgc agattacagc actgcaaaat ggagaaatag
     4561 atattgctct tggcctcaac tctgaaacgg ccaaacagtt agcaggaaaa gaaggcgtga
     4621 ctattgctaa cggttccacg gctttaatga ctttcctggt aatgagtcgt gatgaaacga
     4681 tgtctaaaga aatgtccaac cccctagtgc aggaagctgt acgtttcgcc ctagactatg
     4741 aaggttatct gaccctaggc ggtgagggct gcaccatccc cctaaacttt gtccagcaag
     4801 gttttgctgg tgcgctgacc cgtaaggcgg acagccgtga cctggaggaa gcccaacgat
     4861 taatggctga agccggttat gctgatggtt ttgagattac gctgacttgc gccaataaca
     4921 attctgaagg tctggaatgg acgaccattg ctcagaaagt taagcaggat ctggcggaaa
     4981 tcaagattaa cgtcaagatt gaaacgctgg aaacaaccct cgtctatgaa aaaatgcgtg
     5041 atgccgcgat gcctttctat gtgatgttct ggtctcccga ttattacgac attaacaacc
     5101 agtttgcctt tcttcccggt acaggcgata gtggtactgc ttacggtaac cgtaccaaat
     5161 gggaacgcac agccgcaaac gaaaagatgt gggagttagc aaataagatt caggtagaaa
     5221 ctgataccac tcaacgtgcc cagtatgccg aagaactaca agagttcttt gccctggata
     5281 atccgtttgc ttttcttttg cagcatgcta aaacctttgc ttacaactcc aatacactac
     5341 agcatgtttc ttacaatgac ctctgcaaaa ttgagctgtg tgaattagta gcaaagtagt
     5401 tctattcaac gctccctttg ctatgaaaga aaggatgtgc cattttggtt cgttttctac
     5461 tcagacgttt ggccttaacc attgtgctgc tgcttggtgt gaccgtcatg gtctttctga
     5521 tttcccattt ggtccccagc aatccggtag cagccaacct cagccaaaca gcactagccg
     5581 atccagaaat tgttgctgct tacgaggcaa aatgggggct tgataaaccg ttatcagaac
     5641 aatacttcat ctacattaca aatatcttta aaggtgattt tggcacctct attcgcaccg
     5701 gcagaccaat cttgcaggat ttaaaacagt atttcccggc aaccttggaa ttgtcgcttt
     5761 tagctatgct gattgccatt gtcttcggca tcagcttcgg cattatttcc gcggtctggc
     5821 aaaataagcc cattgatcag ctgctgcgcg ctatttctgt cagcggggtc tccctgccca
     5881 gcttctggtt tgcgctgctg attatgtctg tctttagcgg tacgctcaag tggttcccca
     5941 gtgggggacg gattaatccc cgtctcttta atcccactgg gggtaccggc ttgtatctgc
     6001 ttgatgccct cagctgggga gattggggga tgttaagcga ttctctggct catctgatcc
     6061 tgcccgcgct ggtgctaggc tttttcacca tgggactaat cacacgacaa acccgttcta
     6121 acctgctgga aacaatgtcg atggactata tccgcacggc ccgcgccaag ggcatgcggg
     6181 aaagccgcgt cgtttgtgcc catgcgctga acaatgcgct gatcccagtg attactgtca
     6241 gcgggatggg tttttgtaat ctactaggtg gcatggtctt tgtggagaaa atcttctctt
     6301 ggccgggcat tggccagtat gcctatcttt cagcaattac ccttgatttt ccggctatct
     6361 gtggtgtttc tttgctcatc gctgccactt acgtcgtcat taatctgttg atagatattc
     6421 tttatggtat tattgaccca agaatacgct accagtagga gaggagaaac atcatgaatg
     6481 ctatcaaaca gttgctaaaa caaaagaact ttatgtttac ccttggcctc atcatctgcg
     6541 tagcgtggat tttagtagca ctttttgctt cattattgtc accctacgat ccagttaaag
     6601 atcaaaattt agcaatacgc tttaaaccgc ctagcagcgc tcactatttt ggtacagaca
     6661 gtttaggtcg tgatgttttc agtcgtgtat tgtacggcag ccgcatctcc ctggccgctg
     6721 gtttggtcac tgtgctgatc tccttttttg taggtctgct ctttggtggg attgccggtt
     6781 atgtgggcgg gattgttgat gattttatga tgcgcttcag cgaaatggtg caatccttcc
     6841 cgccgctgat tctagctatg gtcattgcgg cagctttagg acctaacatc accaattcca
     6901 ttattgccat ggccattatc tggtggccaa actatgcccg gctcaccaga agcattgtga
     6961 tctcggtaaa ggagaatgac tatatcacgg ccagccgagt gctgggggca acacatttac
     7021 gcatcttgta caaggaaatc ttgcctaaca gtcttggttc actgattgtg atgtgcacgc
     7081 tggatcttgg taatgccata ctgatgtttt ccggactttc ttttttaggc ctaggtgtac
     7141 agccccctgt tgcggaatgg ggagcgatgg tcagcgatgg ggtgctgacc tttaattact
     7201 ggtggatttc caccttcccg ggactggcaa tctttagtgt cgccatgggc gccaatttta
     7261 tcggcgatgg tttgcgagat tacctggatc cccgcatgcg cagacaaatc taaaactttg
     7321 cgcccaacta aggaggtaaa gataatgaaa aaagtaactt tagacagtgt gcccatcacg
     7381 ggcttgcctt tatatgaaat gcagcagctt ttccagatta atgagccctg cagtgaaatt
     7441 aaaatcagct acatcactct cttaccaggt caacgtgtac cggaaagcgg caccggtgtc
     7501 catgaggagg acgaatattc ctactttatc gaaggcgaag tctataccga aagtggtgac
     7561 tatcaaggcg tctgcggtaa aggtgacgcc acgctgattc ctcagggtga agcgcactgg
     7621 tcagaaaatc tcacagataa accatgccgg attgtctgtg tcctcgtgaa aacacaaaag
     7681 aagtgagcag agagcgacga tgaataaaca acgaattgaa cgtgtgatcg ccacgatgca
     7741 gactgcgggt ttgcagcaaa tgattgtttc agccaccgct gctctctatt atttaacagg
     7801 ttattgggtc gagccgcatg agcggatgat tgctctgtat cttgatgctg ccggacaagc
     7861 agtgcttttt ggcaatgaaa tctttggctt acccagtaca cctgcgctgc cgcttgtttg
     7921 tcacaaggat agtgataacc cagtcgccga cttagcaaaa catgctgccc cgggcaaact
     7981 aggcatcgat aaattctggt acagcaagtt cctggttgag ctgttggagc tgcgtcccga
     8041 tatcgtaccg gtgcatggtt ctgcacccat agatctagcc cgcaccacta aagatgaagc
     8101 agaaattgcc caaatgcgcc acagctcctt gattaacgat caagtgatag ctgccgtaat
     8161 tggcatgctt caggaaggta tcacagaaaa ccagctggca tcccaggtca atcagcaatt
     8221 tctccagcat ggtgctgata gtgaaggtcc gcaactggtc tgctttggtg ccaatggcgc
     8281 tgacccccac cacagtggtg atcagaccaa actccaacca ggtgacagcg taacctttga
     8341 tattttcacc cccattaatc gctactggtg tgacatgacc agaacagtct tttaccagac
     8401 ggtaagtgat cgtcagcgcc aggtttatga actagtaaaa caagctaatg aagcagcgat
     8461 tgcggccatt aaaccaggcg tgctgctttc tactatcgac cagacagcgc gggagattat
     8521 catccaaggc ggctttgccc cgtattttac ccaccgttta ggtcacggga ttggcctgga
     8581 atgccatgaa ccacccgatg taagtctggc ctctgagatc ccgctgacac cagggatgat
     8641 cttctctgtt gaaccaggca tttatctgcc cgatgagttt ggtgtgagaa ttgaagattt
     8701 agtcttagtc accgcagctg gctgtgaagt gcttaatcat tactccaaag agctgcaagt
     8761 aattaaataa gcccagacaa aagctccagc aggaaattgc tggagctttt atgtagttaa
     8821 tttaacaaat tatcctgacc aattagacag gaaaatagtt ttataagtag aaaaaagtag
     8881 agagagccgc agacagctag cggtattttc agaagagatt ttaaaccgct agaggaggag
     8941 tttagcgatg aaacgacagc agcgagctag cagcagaaaa gctcccagaa gaaatagaag
     9001 cctaaccttg ctcatcacat tactggcggt ggtgattttg acaggttatt gttaccgcga
     9061 taccctttat gcttattggg atgccgatgt cccgccggta gatgcccctg tggaggagcc
     9121 gcccagcggc acccccgctg atactgaacc accggctgcc gaggaaccac cgcctgtgga
     9181 gaagacttcc gtatatcctc ccgcaccagg agaaatcctc cagttagcaa acggcaatga
     9241 cctgttagca ctggtcacaa aagagacaag cttaggacag tataagccga ctgatctggt
     9301 gacagtgcca gctgaaatgc gcttatatga tgtggagtac cagctgcgtg ccgaagctta
     9361 tcagcagctg cgcgccatgt gggaagacgc cggcgccgca ggcgttgatt tcttcgtcaa
     9421 ttccgcatac cgcagctatg ctacccagaa acgtctgttt aacaactacg ctgctaaaca
     9481 cggtgaagct gaagctaaca aatttagtgc ccgccccggt cagtctgaac atcaactagg
     9541 cactaccgtt gatgtaggta ttgctggtca tgtgctgacc aattcctttg gcggtaccac
     9601 acctggcagg tggctggtag aaaacgccca tcgctacggc tttgtgctga gttatcccaa
     9661 aaacagcagc gagatcactg gctacatcta cgaaccatgg catttccgct acatcggcgt
     9721 agacatggcg gcagcctgga gggagtctgg ccagattctc tgtcaatatt tagcccagca
     9781 accgcaacaa tggcagtaag acattgagct cgctaaaatt tagtaaaaag cagatagact
     9841 aacaaaaaag gaagtatttt cacttccttt tttgtttgca gcaatttagt taactgttcc
     9901 cgccagagcc ttttgagccc acagggaatc ctgcatgatt gctctgccca ctccgattaa
     9961 atcagcagca ccacgtgcta ataaatcatc tgcttcttga cgcgaagtga tccccccggt
    10021 taaaatgacc ggcagatcca ctgcctgctt aatagcactg gaaacatcag caaagtagcc
    10081 gggttcacgg gaagtgcttt tcatgtaacc aaagatcccg cctgagatat caagcagatc
    10141 tactcccgcc tcagcaaacg cctgtgccgc aatgacacta tcttcaattg tgctgccacc
    10201 atcttgataa tcacagccgc ctaaccgcag catcaggggg aaactttccc caactgcttc
    10261 acgaaccgcc ttgataatat ctagatgcag tcggattcta ccgtgaagat cgccgccata
    10321 ctcatcagtt ctgtggttag tcagcggaga ataaaactgg tttaataaga aaccatgtgc
    10381 agcatgaatt tctaccgcat caaaaccagc tttttgcaca cgtccggctg ccgcttgaaa
    10441 ttgggcaatc actttttcaa tctgccccac agtaagcgcc tctggtatcc tttgcttgtt
    10501 agggtgggcg acagcagaag gtcccataac ttcgtcagca atgccggtac tgcctgcatg
    10561 gttaatttgt acagcagcct tagcctcatt agcatggatc gcacttgcta gctgctctaa
    10621 accagggatg tcacgatcac tggacacaga catttgtcgc ttactggcct tcccagctac
    10681 agctacataa ctatgctcga taatcaccag ggaaatatat cccccttgtg ttttatcctg
    10741 ataatattcc aatatccctt tggtaatctt tccctcatca tctgctttgg ctgtagccat
    10801 cggcggcatc accagccggt tatgtaaatg aagtttacag ctcaccaatg gcgtatcaag
    10861 ttgtgtcatc ttttatcctc ccatcaataa atctaagtct attatagcaa agtagtcatc
    10921 aggatactaa tcattgtaaa ctaattaatt ctgtactctg cagtatgtat taactatgaa
    10981 aaatggtaac actactagca ggagttgatg ccgatgatac caataccgga attttacatt
    11041 taccctatct ataccggaat tttagcccta accttaattg ccctgctgcc acgtgaggaa
    11101 attaaacgtc ttgtaatcta cggcatagtt tttggcgctg taactgatgt cgcaattatc
    11161 ggtgtcatca gcaacctgct aaaggtggga ggacatatta gctattatcc gtttggggca
    11221 ttgggcgttg ctttctttcc accattagcc tggggtatct ggtttatcct gtttttctac
    11281 ttcctgccta cagatctgcc gtgggtggtg atttatgttt ttgctgccgt cggtacatcc
    11341 gttttgtttt ctaatgtcct ggtaaacatg cagatctttg tttggaacta tggccgcatt
    11401 ttagtcccct ttctaattta tggtttatgg ttcagctttg ccgcctgggc ttataagcgt
    11461 cttacaaagc atcatgccta gtctgcttta atacagctac atatccctct acatcacaac
    11521 acgggtaaca gctactcgtg ttgttattta tctgctgaaa aaaatgcagg aaagctaaac
    11581 aaattgccga actgtaacta taataaatct caagatgtga ggagttaata tgtgcctagt
    11641 agtatttgcc tatcaacaac atcctaacta caaactaatc cttgccgcta atcgtgatga
    11701 gttctacaac cgtcctacag ctcgtgctac tttttgggcc gattgtcctg atctactggc
    11761 cggtcgtgat ttacaagccg gcggcacctg gcttggtgtt accaaaagcg gccgttttgc
    11821 cgccctcacc aactaccggg aacccgcttg ggaagttacc aaccgcaggt cacgcggcga
    11881 tgtggtgcgt aactacctct gcaaccaaat cactcctgcc gaatatgcag aactactgcg
    11941 tcaaactggt acagaatata atgtttttaa tgtgatcttt ggcgatgtaa actcgttatg
    12001 gtattactcc aatgtgacgg gtgagccgct actgttaacc cctggtcttt acggtctctg
    12061 caaccacctg attgatacac cctggccaaa agtggcgcgc agcaaaagcc aactggcaca
    12121 atcacttaat tctaccccac taaacaaaga tcagctccta gctctgctga agaataaaaa
    12181 gcctgtacct gaccatgaac tgccagacac aggcgttggt ttagaatggg aacgcattct
    12241 ttctccagtc ttcgtagagt ccgagacata cggtacccgc tgctcaacca ttctaactat
    12301 agatgaagca ggttccctag attttctaga acgtacctac atcacacatt cccctgtata
    12361 tgaagacgta tcgttttcct ttaaagtata accaaattac tttgctatcc attgcccatc
    12421 ctcaagttga tatgtgctgc tgttaggaga gatgggccac tctagcaaat agtcattaat
    12481 aagttcagct tgcagctcta aagtatccat tttttgtgga tcatttgctt catctgctaa
    12541 taaccattgt aatgtagcac tgttgagaat cctgatattc gcttcatcaa cccggtcacg
    12601 caccagctgc agtgttgcat tataaaacgg tgccgccaga gcaattatca ggataataat
    12661 tactagtact agcataagtt ctaggtaaaa gcgattgttg ttagaaaagg ggatctcact
    12721 ttttgctttg ggcatgatgt accactccgc agtatttgct gatcattcta ttggcagaat
    12781 accataagta ccttaaaatg gcaatatatt tgttagttcg acaaagctgt aaataggaaa
    12841 taagtcctag cagaataaag actattgttt gattactgta cacctaaaag cttgttataa
    12901 ttacaataag taatattgta tagttttgag gtgcaagatt gtgggtaagt ttttaaaaaa
    12961 attattccaa aagcaacaag gacaagccat aacagagatg gctcttacac tgcctatctt
    13021 attattaatc ctgctgggaa ttatccagtt tggctttatt tttaatggac aaattgctgt
    13081 agccagtgca gcacgagaag gagcacgttt ggctgcggta ggtgcgcaag atgccgagat
    13141 taccgctaca gtgcaaagca cgcttgccgg gatagcttta ttagacaacc agagtactaa
    13201 tgttacacct tccggtaccc gaatatttgg ccagcaggtg acggtagaag tggctgccag
    13261 taccaacatt gttgttccct ttttcaattt agttctcgga gaaaatttta accacacctc
    13321 cagtgccacc atgagggttg agtttgtgca gggaggctct taaaatgaaa ttcttacaga
    13381 gactgttgag ggaggaaaag gggtcagcga tggtgcttgt caccattgcc atggtgatat
    13441 tactaggttc ctcggcacta gtcattgatt acggtcagct tgttttaatc agaagaaagt
    13501 tagttaatgc ggctgatgct gccgccttag ccggcgccca agaattgtta tcagaaccag
    13561 cttccccaag taatgctatg agtacagctg aagcgtatgc cattgctaac ggtgttgatg
    13621 ttgaccaggt gacggctgaa gtaacacatg ataaaagtca gattacagta actgtaactg
    13681 gcatagtaga ctacactttc gctcgtgttt taggcttcga gcagggaaat gtatctgcag
    13741 aagctaaggc cgcagtcggt tcaattcaag gcatgaccgg cgttgcaccc ctaactattg
    13801 tagagcagag ttttgtcaaa ggccagtcgt atgaattaaa atctgcttct ccatctgaga
    13861 tgggccccgg caactttggc gtgctcgccc tcggaggatc gggtgcctcc cgttatgaag
    13921 ctaatcttcg tgatggttat agcggcaaca ttaaaatcgg tgatttatta gacactgaac
    13981 caggtaatat gtctaacccc actaaaagag cttgtactga ccgcattaat gcttgtaacc
    14041 tgggctgtac ctaccaaaac tttaaacctg attgcccgct tttagtatac attcctgtca
    14101 tcaaacaacc caccggacaa ggtcggcaag aagtggaggt agtaggcttt gcagccttct
    14161 ttctagataa acatcaacct cccggccaag gcaatgaaag ctcaattaaa ggttggttcg
    14221 ttgacaccat tgtccccgga caaataaatc ccggcgagac aggctacggg gtttctgctg
    14281 tgaatttaat taaatagata gtaatgacct caacacaaaa aaaccggact ttggcatccc
    14341 ggttttttac gttatcaagg ttgtttgcag atattatctt ttgtagcgaa taactgttcc
    14401 tgtagctgct ggcaacccag ttagattgtt tagatgtact tgtgattcta cagagtacgt
    14461 ctgggtacct tcctgcccac taacgatgaa agtaacaaaa ttgttcccgg cagcagtttg
    14521 ctgtaaagtg aaggtaagtg agaggatgtt tctgtttgta aaagtgctag ggttggtagg
    14581 attggcatta gttttgtgca taatattatt attctccaga tagaaatact gattgtcagt
    14641 atctccactg ccttcagagg cactgggtac gatttctatt tcagtggcat tccgaagttc
    14701 tttggtaata aagtcaccat taaggcgaat atccctttgg atattagact gatcttctcc
    14761 tacgctagcg gtacgtgttc caaaatgata aaatgggtac ccgacagcaa gtacaataag
    14821 taagatcgtc atggtaatga tcagttcaac taaacttaag cctcgccggt taagggaatc
    14881 tttacacatt tacagccctc ctcagctttc cgggatgtgt tcgataatat gaagcacggc
    14941 actgcttgga aatccggtta gggcactata tcctccgctt gtcgcaggat tactcaagct
    15001 taatttaacg gtaaatgatg ctgtttctgt attgttgggg tcagtatcat aattaataac
    15061 ttcaacagga aaagctttaa ctgtctcacc aggagcaaaa gttagtgtgc cacttgtagc
    15121 tttataatga gttccaggga ttgcggaaga aaaatcatca acagttttat aagagatcgt
    15181 agcagtacta tctgtaattc cctttcgagt gaccttaatc tcaattaaat catcatcttt
    15241 aaaagcctta tattcttcta aggcaaactg tagccaaaca ggttgttctc ggtaaaaagt
    15301 tttaataacc acagtgcggc cgtcttcata ttcatgttct aactccacaa tagcgccagc
    15361 tacaccttca atatcactga caggagcaat gtttgctgtg gtaccactat cgccagctat
    15421 tgctttttcg atctcttcct gtacatagaa tttagcacta cttcgcctac cagcaacgaa
    15481 aataccacca tagctgcttg aaaataaggg gactaaagcc gcgatcataa tcactataat
    15541 gactagtgca acaagtactt cgaccaaagt catgcccctt ttgttaaggg taagtaattt
    15601 cattatctgt caccccatgt aacagaagtc acagtcggca gagcttctgc cattttaaaa
    15661 acttcctcta gaacatcagg attattaatg cgaattaagt tattattgtt aatttggttt
    15721 tctccattat gaatagctgt gccatctttg aaaaaataat atttattatt tacgtttaga
    15781 attgtattcc agctattccc atgactctct ttgacatctc caccaaagta aacaatacct
    15841 acatactcgt tacttacaaa cgtattggtt tgaacaccat caattgtaaa gtcagagcca
    15901 ctaaacttta tacgaataga ggtactataa gtatgagggc cagcagatgc cgaactattt
    15961 actataaaac gatgttttgc tgaatcttta aggttaggat gtaaatacag taaagactta
    16021 gtgtgatcac ttaaatagat agaatcgtaa aaagatacaa taccagcatg aagatgtaaa
    16081 gtggcattat tacctatctc cagacttggc gccgcatcaa taaatgcaat aaatgatgca
    16141 taaagatctg ttaacccgct agtgtgcttt atcgcatgac caacgttctc cttattagat
    16201 acctgagcga tattacctgt ttgatcactg gggtttttgt cattggtatt aacaatataa
    16261 ccattattca gccactgagg cgggttaagg tttagggcag ggaagtgaaa gtttactatt
    16321 gccgcctctt tttgtgttac ttcggcagct gtgccgttag aaatgatttt tacagagcta
    16381 tttgttttat cgacttcata atttacatca aatgtacctg tatcaacagc ggaaattgaa
    16441 ggaatgtatg ttagttttgt ttcaagttca tcgattgaag tgatggttga aactgagtta
    16501 tacagtacat tttcaaggtt acccagacca acttcgacac cagaacgagc taaatagtag
    16561 gcttgtgtca tctcttcagc ccgtgctact tgcatggtgt ccgtcataaa gtactgccac
    16621 aatactgttc ctaacagggt caaaacaaac aaaacaagta atgcgattgg catggcagct
    16681 ccacgattat ttttcaacat aaaaaagccc ctttcttaaa catatcaatg agatatcaca
    16741 tgaggctaag taattgatgt ctgtatttcg ctttaattat aacgtacaac taaaatgaat
    16801 actatatcct tgaatgtagt aagtgataca tcaacacctt cttttgggaa aacaggggga
    16861 ggataatatt tacttgaatt tgaattttgg gtgcctcgaa aattatcatt ctggttttct
    16921 ttagtgggga gtatctctcc cgtagcttcc acgcttacat tatctcctcc atcttcatcc
    16981 tttatatcca cttcgtgtag tagcaaagtg tgcttatctt tagatgtttg cgcaatcgca
    17041 ttagaaaaca gattcggatt atcgtctttc gacaaaatga gtgtcacggc ctgttctacg
    17101 ccgttagccg tgccgatgga ttgaatttct ataatatctt cagtttcgtt gacgtcggtg
    17161 cgaaaagtag catccccaac gtttcctgtg gcatacgtag atgaatttgt tttcgctgca
    17221 acatccgcaa ttttgccagg gttttttatc agaaaatcag ccatggcgtc cgcaccagct
    17281 cttgcggcat aatgagcctc tagcttattt gcttctttag caacttgtgc agcatcagcc
    17341 acactgtatt gtattaaagt agctcccaat aagcctaaaa cgagcatgat taccaaaata
    17401 agcggtaatg cagctcctct gtcgttttca tacattattt aacagccccc caataacggt
    17461 agttttacta ttgtatattg taatacaggc atacaataaa ggtagacatt caaatatttg
    17521 taatagttat accagtatag actaaaaata gacttttatt tgtgtttttt ataaaaaata
    17581 cgacgctctg taaaaataaa cattaggcaa ttatcgcatc cttataaaca aactgtaact
    17641 ccttaaaatc cagacaaccc aaaacaatac ccgttaatgg tcgaatgctg cagaaaagag
    17701 gagtagatca aaataagtta ttggaatcta ccgccactat ataaaaaata tttattattt
    17761 caagggaagg
//
LOCUS       DASRMS010000134         3308 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_236132, whole genome shotgun sequence.
ACCESSION   DASRMS010000134 DASRMS010000000
VERSION     DASRMS010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3308)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3308)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3308
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236132"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="VFC74_06225"
     CDS             complement(<1..240)
                     /locus_tag="VFC74_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24967.1"
                     /translation="MAQNLKPDLADKRREKRLNRSRKNAPLGKYTKSAPPPLKVAEKI
                     RANRAERYELLASARALFLHEGKKENLKYPHDWHRT"
     gene            444..1061
                     /locus_tag="VFC74_06230"
     CDS             444..1061
                     /locus_tag="VFC74_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK24968.1"
                     /translation="MKKIIVSLFCTLLLWTNYSLAAHIFTAEEVRLITDDANTGNPKA
                     QHLLGRLYSNGQIKGEFQGEGIFQNLTEAKNWFEKAAIQGSSKSQTELGTMYLRGLGV
                     KQNHKKGEDWLRKAALQGDELALLSLGNMYYFGKGVDKDYALAKSFYEEAINKSNSSD
                     GLFFLAVMYTTGKGVPKDLKKAKELAARSCVGGSQLGCNAFEKLN"
     gene            1973..2908
                     /locus_tag="VFC74_06235"
     CDS             1973..2908
                     /locus_tag="VFC74_06235"
                     /inference="COORDINATES: protein motif:HMM:NF023168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HZK24969.1"
                     /translation="MQTNTRAKVTTEQINHAIEQLNSEGLRPTIRAVRERLGAGSPNR
                     IQPLLAAWHDKNKPAETAQAALSDSAQQTLADLLARERSTAVAAKLEEITELKTQLDE
                     ITQTGQQLEDDNERLENENAQLDEQRNEAATLANARQQRIEQLEAEQKRLQTQSQELT
                     QQLATAQAQLAMQQQQSQQQQAQLEQQTAAVTEANQQTAAAQQAAAVAQAKQQAAVET
                     ATAAQKRIEKLEQQIDKQQAQANAKLDALQVRLDDVQQQRAADAQAHTKQLAQRDEQL
                     HVEHTKNAQLMTQVDELTDKIKTLSTKKPAAQKRD"
BASE COUNT         1052 a          776 c          745 g          735 t
ORIGIN      
        1 ggtgcgatgc cagtcatgcg gatattttaa attttctttt ttgccttcat ggaggaacaa
       61 ggcacgcgca gaggcaagca attcgtagcg ttcagcccga tttgccctga ttttttccgc
      121 cactttgagg gggggggggg cggatttcgt gtacttccct agaggggcat ttttgcggct
      181 gcgatttagc cgtttttctc gccttttatc ggctagatcg ggctttagat tttgagccat
      241 tgaatcgcgt ccctaacatc gggcgcacga tacttgaaaa gcaatgtttt cttgtacata
      301 atgattccca gagattgaga aaccgcgcga ggcttgcagg ccgttgaccg cggtttttta
      361 ttgtctgcct ttcagcaatt acgtcaataa ttgcacatca cccccgcctc gtcaaaaagc
      421 acatctgtat caaagggatt tacatgaaaa aaattatcgt atctctattc tgcacactat
      481 tgctatggac taactattct cttgctgcac acatattcac agctgaagaa gtaaggttaa
      541 taacagacga tgccaataca ggaaacccaa aagctcaaca tcttttaggc agactttatt
      601 ctaacggtca aattaagggt gaatttcaag gtgaaggaat atttcaaaac cttacagaag
      661 caaaaaactg gtttgaaaaa gcagcgatac aaggcagctc aaagtcgcaa acagagctag
      721 gcaccatgta cttaagaggt ttaggtgtaa agcaaaacca taaaaaggga gaagattggc
      781 ttagaaaagc agcattacaa ggcgatgaac tcgctcttct ctcactagga aatatgtatt
      841 attttggaaa aggtgtagat aaagactatg cattagcaaa aagtttttat gaggaagcaa
      901 tcaataaaag caacagcagt gatggtctgt tttttttagc agtaatgtac acaacaggca
      961 aaggcgtacc taaagattta aagaaagcta aagagttagc tgctaggtct tgtgttggtg
     1021 gttctcaatt aggatgtaat gctttcgaaa aattaaatta gcaataaatt tattctcaat
     1081 cggtattcat ccataaaccc tactatttat aattaaaaac cccgagtaaa gaagggaaag
     1141 taaaaaccct tagtaaagag agagggagtc taaaaccctc catattaata aatcaaaggg
     1201 ggtttttatg aacataaatt tatttgcaga aacagtatta aacagcccaa cagaatctct
     1261 gatagcaggc tctgtatttg tcttaagtat agcgatactt gtttttctta tggaaaggcc
     1321 gtaaccattg ctgtccagca caggacgcgt tgatccagaa gcgaataagg aaaagtgaag
     1381 taggcctact tcaccctatc ctgcccagca ttcaatgctt atttttccat taaaaatggt
     1441 tatgccatcg tgaataactc agcaaactag cggctttact taaaaacagg ttaaaaccag
     1501 caaatacgcg cattaatcac gctttaataa tgcttcttca cgctttattc acgtttatta
     1561 ccgctttttt cattctcacc catcaaaacc acccccacta acgcgccccc ctgatggggg
     1621 cttattcact cgccgtgggg caggcggtca ggggaaaagc accctgacca ccagcccgaa
     1681 gtgttggggt cgttacgatg cgtcaagtgc cgctacgctc aaatgctcac ccgttcagac
     1741 tcgctgcgct cgcttagctg cggcttgcgc tttgcacttg acccatcttc acttaaaagc
     1801 aatgcaggcc gtggggggtg ctggacggct tgtgaccggt ctaacttaat caattatttt
     1861 caaaataatg tttgacgtat catatcaata agcttatcat atcatcatat catatcaaat
     1921 atgatacgat atcatgatat gataaggcca ttgatatgag tgaggttaca aaatgcaaac
     1981 aaacacacgc gcaaaagtga caaccgagca aatcaatcac gcgattgagc agctaaacag
     2041 tgaaggtctg aggccaacga ttcgcgcagt acgtgagcgt ctaggcgctg gtagcccgaa
     2101 ccgcatacaa ccgctactcg ctgcatggca cgataaaaac aagccagcag agacagcaca
     2161 ggctgcattg agcgatagcg cacagcaaac ccttgctgac ctgctagcac gtgaacgaag
     2221 cacagcagta gccgccaagc tcgaagaaat caccgagcta aaaacgcaac tcgatgaaat
     2281 cacacaaaca ggccagcagc tcgaagatga caacgagcgc ctagagaatg aaaacgcgca
     2341 actggacgaa cagcgcaatg aagcagcaac acttgctaat gcccgtcagc aacgcattga
     2401 acaacttgaa gccgagcaaa aacgcctaca aacacaaagc caagaactaa cccaacagct
     2461 cgcaaccgca caggcgcagc ttgcaatgca acaacagcaa agccagcagc aacaagcaca
     2521 gcttgagcaa cagaccgcag cagtcacaga ggcgaaccaa caaacagcag ctgcacaaca
     2581 agccgcagca gttgcccagg caaagcagca agccgcagta gaaaccgcca cggcagcaca
     2641 aaaacggatt gaaaaactag aacagcaaat tgataagcag caggcacaag caaacgcaaa
     2701 gctggacgct ctacaagtgc gcttagacga tgtacaacag caacgggcag cagatgcgca
     2761 ggcacacaca aaacagcttg cacagcgtga cgagcaactg catgtagagc atacaaaaaa
     2821 cgcccagctc atgacgcaag tggacgagct gacggataaa attaaaacct tatcgactaa
     2881 aaaaccagcc gcacaaaaaa gagattaaga gtgaagtcgg cgtcaagggc gaaagcggaa
     2941 gccgcagcgc aagggagcgt agcgagtgcg aacgggtgag cacttcgggc gtagccacac
     3001 ccttgatgct ggcagatcga cccaacgctt agtgctggtg gttggggtgc ttttcccccg
     3061 accgcctgcc acgtggcgaa cgtggggagg caagcgaagc gcgctagggg caacgcccct
     3121 tagccacaca agtactatat tttattttgc gtatatattt cggtgtcccc gctatgtaca
     3181 cgctgaagcg tacatagcgg agccagtata tacgctgcat tcagaaatta gacgcaaaaa
     3241 ataggcgcaa attgacggtt acgccaatac tcagtatatt caaaattcct gaagccttag
     3301 cgctggct
//
LOCUS       DASRMS010000135         1897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_572504, whole genome shotgun sequence.
ACCESSION   DASRMS010000135 DASRMS010000000
VERSION     DASRMS010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572504"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..320)
                     /locus_tag="VFC74_06240"
     CDS             complement(24..320)
                     /locus_tag="VFC74_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24970.1"
                     /translation="MKELLLIIFLAILVEYIVNIIKPLIPDVNYPIPLILSLIVGIGL
                     ALLVQIDILSALGFEPRNIHIGCVVTGLVVAGGASAVHELFAKLRASRDDINCE"
     gene            complement(317..793)
                     /locus_tag="VFC74_06245"
     CDS             complement(317..793)
                     /locus_tag="VFC74_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24971.1"
                     /translation="MEILLADHILNVDEVLYWLGAILTIFAVVTAIYVPVKKMLKKYD
                     KALQKTEKTLENHADINAQYQKLIDESKADRKQLNEDVSAIKKATLTQIKLELTSICD
                     NMINRGYAYLCECEKAEHLFDAYEPLGGNSGMAAKVERVRALPIRRAPLENKEIEE"
     gene            complement(1029..1733)
                     /locus_tag="VFC74_06250"
                     /pseudo
     CDS             complement(1029..1733)
                     /locus_tag="VFC74_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004103219.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
BASE COUNT          448 a          478 c          330 g          641 t
ORIGIN      
        1 atttatttat attttatatt gtgttattcg cagttgatgt cgtcacggct ggctcttagc
       61 tttgcaaata gctcgtgcac cgctgatgct ccacctgcca cgactaagcc tgtgactaca
      121 cagcctatat gaatatttct cggctcaaag cctaatgctg atagaatgtc aatttgcacc
      181 agcaaggcca gcccaattcc tactataagc gataatatca gcggtatcgg ataatttaca
      241 tctgggatta gcggtttgat gatgttcacg atgtactcga ccagtattgc caaaaatatt
      301 attaataata actctttcat tcttcaatct ccttattttc tagtggtgca cgtctgattg
      361 gcagtgctcg caccctttca actttcgccg ccatgcctga atttcctcct aatggctcgt
      421 atgcgtcaaa caaatgttca gccttttcgc actcacacaa ataagcataa ccccgattaa
      481 tcatgttgtc acagatacta gtcagctcca gctttatctg tgtgagtgtt gcttttttta
      541 tggctgacac atcttcgttt aattgctttc tatccgcttt gctttcatct attaattttt
      601 gatattgtgc attaatatca gcgtgatttt caagcgtttt ctcagttttc tgcaaggctt
      661 tatcgtattt tttcaacatt ttttttactg gcacataaat tgccgttacc accgcaaaga
      721 ttgttaaaat cgcgcccagc caataaagca cttcgtctac atttaatata tgatccgcca
      781 gtaaaatttc catgtcacac ctttactaaa tctttctcct gcatcgccgc tgttacttgc
      841 cccttgagcc cgataactat ccgattcccg cgcggtactt ccaacacgtc aaaagtagtc
      901 gtgtacaccc acccttgcag tattccgcct gtatatgttt ttgcaccctc gcggacagtt
      961 acacgatcgc cttttttaat cgcaggtact ggcttcggct tatcaggtgc tttcgcttgt
     1021 gcagtgacaa cataagcgtc aaatcctgct tttttaatct gtgctgcata attatcagcg
     1081 ttgcttttta ctcggaaagc gcctacttgc actctgtaaa gtgtatcacc aggcgtcggt
     1141 ttcggcgtcg gcgtcatttc tgccgctata tctttgcgca ttttatccag tgtaatcccg
     1201 tacttaccaa gccagtgcct gacatcgcca tgattgctcg ctatccctag cctcgcaccc
     1261 tcggcatgat cgatgatcac gtttttgccc agcgggtcta atttatattg tttgcataga
     1321 tatgccgaaa attcaaccgc ttccttatat acagccattg cataattttt atctttcgta
     1381 ttgtcttcac agatttcaaa gcctatatat cctaagcgat tagcacagcc tttattgccg
     1441 ctaccactgt gccacccgac catatcccaa ggcaatgtct gataagtggc aatacatcca
     1501 tcctgcaatt taccgataaa agcatggcag caaacttgcc tgccacttgg cctgaagttg
     1561 ttccatgaat tgttgtagat gttcttgccc agtagtccat catctggctg aacatacctt
     1621 ttcaagaatg gattgttcgc gcctgtactg tgccacatta tgcctatcgg cgtcatttcg
     1681 tggccgatga tatagcactc gtttttcgtt gctaactgtt tttttaagtt cattttaatt
     1741 tccacccttg tggataatct gttggtgacc acgtattgcc atcaattata cattcatata
     1801 ctttaccctc aaatatcacc ttgtcaccta cactataagc atcatgactt ccagtaggtt
     1861 gtacccattc aggtattacg ttttctggca tcatact
//
LOCUS       DASRMS010000136         1188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_370095, whole genome shotgun sequence.
ACCESSION   DASRMS010000136 DASRMS010000000
VERSION     DASRMS010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_370095"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VFC74_06255"
     CDS             <1..498
                     /locus_tag="VFC74_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK24972.1"
                     /translation="KQLLKTAEGNDMFSEFGRVGRDVTLRYTPNGMAVAEIALAYEYG
                     RKDQQTGKRPTQWVEVTMWGKTAEALSQYLTKGKQVFIEAKDVHIQTYDKNDGTQGFK
                     LAAEFVGIKFAYDSQGQQQSQAPTQQQRPAQQQSQQAAHNMAFDDGQDIPFANPYRGR
                     RALLI"
     gene            542..730
                     /locus_tag="VFC74_06260"
     CDS             542..730
                     /locus_tag="VFC74_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24973.1"
                     /translation="MKWKRRCKYTAASECGTYLVAGYRVDGRILYRASRGGVFVTDPL
                     DSMDKINKALKSRYSIRK"
     gene            815..988
                     /locus_tag="VFC74_06265"
     CDS             815..988
                     /locus_tag="VFC74_06265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24974.1"
                     /translation="MSVLKVATKEDFKVGAVLVCIAEGWEFKLTAKYDDGIWEARQTV
                     VFETEANCYYVKQ"
BASE COUNT          380 a          244 c          307 g          257 t
ORIGIN      
        1 aaacagttat taaaaacagc agagggtaat gatatgttta gtgaatttgg ccgagttggc
       61 cgtgatgtaa cgcttagata tacacctaac ggaatggcgg tcgcagagat tgccctggca
      121 tacgaatacg gtcgcaaaga tcagcagaca ggtaagaggc cgacgcagtg ggttgaggtc
      181 acgatgtggg gaaaaacagc agaggcgctg tctcagtatc ttacaaaagg caaacaagtc
      241 tttattgaag ctaaagacgt gcatatccag acgtatgata aaaacgacgg cacgcaaggc
      301 tttaagcttg cagctgagtt tgttggtatt aagtttgcgt atgacagtca aggtcagcag
      361 caaagccaag ctccgacaca gcaacagcgt ccagcgcaac agcagagcca gcaggcggct
      421 cacaatatgg cgttcgatga cggtcaagat attccatttg ccaacccgta tcgcggccga
      481 cgggcattgc taatctaacg caacaaagcc cgccaccata cgggcttttt aatggtgacc
      541 tatgaaatgg aaacgccgct gtaaatatac agcagcctcg gagtgcggca cttacctagt
      601 tgcaggttat cgagtagatg gccgcatact gtacagagcc agccgtggcg gcgttttcgt
      661 tactgatccg ctggacagca tggataagat caataaggcg ttaaaaagcc gctacagcat
      721 tagaaaataa tttaaaaagg ctttactctg tgcgcggtac tgcatataat ggacacaaca
      781 aagcgaaaca ctaaacgaaa gaaaggaaat acaaatgagc gttttaaaag tagcaacaaa
      841 agaagatttt aaagttggcg cggttctggt ttgcattgca gagggatggg aattcaagct
      901 aaccgctaaa tatgatgatg gtatatggga ggcgcgccaa acggttgttt ttgaaacaga
      961 ggcgaactgt tattacgtta agcagtaagc agcctgaaaa atatgataaa actaagtgcc
     1021 cttcggggca catactcaca cgaatgtaag gaagttaaaa atgaaaacta aatacgcaga
     1081 tttaattgca gagttagaaa aaacagccag cgcatacgct aagcttgatg ctgcggccgc
     1141 tgagtacgag gctgcaatcg ctggcaactc cagtgtgacc ggttaccg
//
LOCUS       DASRMS010000137         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_438384, whole genome shotgun sequence.
ACCESSION   DASRMS010000137 DASRMS010000000
VERSION     DASRMS010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438384"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..194
                     /locus_tag="VFC74_06270"
     CDS             <1..194
                     /locus_tag="VFC74_06270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24975.1"
                     /translation="LKYCLYIVAHNTLLTIAFQQEVYKKNTTERSPKTFFFTIREEKG
                     GSKGQFGVVLHLAELRNSV"
     gene            complement(853..2154)
                     /locus_tag="VFC74_06275"
     CDS             complement(853..2154)
                     /locus_tag="VFC74_06275"
                     /inference="COORDINATES: protein motif:HMM:NF018513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0031317 - tripartite ATP-independent
                     periplasmic transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HZK24976.1"
                     /translation="MAVEIGFYGLAILLGLLALRVPVALALIAVSLGGVTFMFGWDTS
                     ISLLAAKPYEFLSKWTLSAVPMFLLMGFVCYHTGLTGGLFNAAKVVFRWLPGGLAIAS
                     VFASSGFAAVCGSSVACAAAMGRIAIPEMVRSGYKPSFACGTIAAGGTIGALIPPSIL
                     MIVYGVFAQVSITDVFLGGIGIGIVTALSYTMIILLVSWLRPDIVPREATDVQEGYSV
                     GQALYDIWPVLALGFLVFGGLFGGFFTATEAGAVGAAGAILISLGQRRLDMEKAKLAV
                     LETLSTTAALLIIGIGASMFTVFLSLSGVAGYLSLAVTGWGPSYLELMLIVVVIYLLL
                     GLFMEPFGAMLITLPVLLPVFELYDVSLIWFGVLLVKLLEIGMITPPVGMNIFVIRGV
                     AGQYASLVDIFKGVTLFLVADLIVVALIIFYPSIVMFMVGS"
     gene            complement(2178..2669)
                     /locus_tag="VFC74_06280"
     CDS             complement(2178..2669)
                     /locus_tag="VFC74_06280"
                     /inference="COORDINATES: protein motif:HMM:NF016200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HZK24977.1"
                     /translation="MNWLVRLMDRAAVLLALVGTLGLVAMMLHISLDIILRSTMSVSI
                     PATLELVTRYYMVSLALLPLAWVEWRKEMIFVEALGGFFGDIGVRVVDVLVSVLSAGI
                     YVVLTIATWSKAIEQYEIGSYVMSLSFPMPVWPTYFILPIAFGMAAMVSIVRIPLIAK
                     ISH"
     gene            complement(2755..>3145)
                     /locus_tag="VFC74_06285"
     CDS             complement(2755..>3145)
                     /locus_tag="VFC74_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24978.1"
                     /translation="GLFLPAYFPADLPEINLIGDLAFLGNNPQAMAAAMTEYVVTCDA
                     CQAIEPSQELLTATAVFVENDLVFAAREASERYKVKNADAKIEHFRQLVDKWAKIAEA
                     VNNDATKMAEAQQREIWDQVNFSTYGI"
BASE COUNT          972 a          824 c          662 g          687 t
ORIGIN      
        1 gcttaaaata ctgcctctat atagtggcgc acaatacatt gttaacgatt gcctttcagc
       61 aagaggttta caaaaagaat acaaccgagc gtagcccaaa aacctttttt tttacaataa
      121 gagaggaaaa gggagggagt aaaggtcagt tcggagttgt actgcatttg gctgagctta
      181 gaaattcagt atgactgcag actattattt aatggcaacg cacgacgagt tttcaggctt
      241 cttatggact gagacccagc aggattaata tagaataatt tagcctgagg cttataacta
      301 gtgctattta gtacacaata actttactcc tttagtaatg cctgctgtaa aagctccact
      361 gtacaatcag gcttgccaaa gcggctctcg atattactcg ctgaaataac agcttataaa
      421 agcttccatg tataactaaa aaccagcaaa tggcgccgat ccgaaaagcc ctaggctgac
      481 agattgttac tcgaataccg tctaacgaat cgttgatttt cctttgagat aatccgaacc
      541 agaaagtgag tatttaagac cctagctgcg gctaacgcct caccaatata gagcttaact
      601 tgggtaattt ctcgccagga aaagagctct tggctaaata aagaacgact acctcatagg
      661 ctatctctat aaatagtttg ctgagcttct ttatggaact ctcatactcg ttcgggctgc
      721 cgtctgaacg agtatgagaa gagcaggaca ttatttagcc aatcggatac gaagtgcaat
      781 tacactcaat taaacgatac aacactggct tcaaaaactg tcagtgtatt tacaagctgc
      841 ttggcacagt cattagctgc cgaccataaa cataacaata cttggataaa agataatcag
      901 cgccactact ataaggtcag caacaagaaa cagcgtgaca cccttgaaaa tatctaccaa
      961 tgatgcgtac tgccccgcaa caccgcgaat aacaaagata ttcataccta caggaggcgt
     1021 aatcatgccg atttccagca gtttgaccag cagcacgccg aaccagatca ggctgacatc
     1081 ataaagctca aaaaccggca ggagaactgg gagcgtaatc agcattgcgc caaaaggttc
     1141 catgaacagc ccaagtaaca ggtagatcac caccacaatc agcatcaact cgaggtaaga
     1201 tggcccccag ccagtaacgg ctaaagagag ataaccagca accccgctga gactcagaaa
     1261 tacggtaaac atgctggccc cgataccgat tatcagcaat gcagcagttg tggataaagt
     1321 ttccagcacc gccagtttag ctttttccat atccagacga cgctgaccca acgaaatcag
     1381 gatcgcacca gcagcaccta ctgcccccgc ttccgttgcg gtaaaaaaac caccaaacag
     1441 tccaccgaaa acaagaaagc ccaacgctag cactggccag atatcgtaca aagcctggcc
     1501 gacactgtaa ccttcctgca catctgttgc ctcgcgcggc acgatatccg ggcgcaacca
     1561 gctaacaagc aaaataatca tagtgtagct caaggctgtc acaataccga tgccgatacc
     1621 gcctaaaaac acatcggtga tcgagacctg ggcaaaaacg ccgtaaacga tcatcaaaat
     1681 gctcggaggg atcagcgcac caatagttcc ccctgccgca atagtgccgc aagcaaaact
     1741 gggtttgtaa ccactgcgca ccatctctgg gattgcaata cgtcccatcg ctgcggcaca
     1801 ggcgacactc gaaccacaga ccgcagcaaa gcctgaactg gcaaatacac tagcaatagc
     1861 gagaccaccc ggcaaccagc gaaacacaac tttcgccgca ttgaataacc ctccagtcaa
     1921 ccctgtgtga tagcagacaa atcccatcag tagaaacata ggtacggcgc taagtgtcca
     1981 ttttgacaaa aactcatagg gcttggctgc cagcaggctg atggaggtat cccaaccaaa
     2041 cataaaagta actcccccta aggagacagc tatcagcgcc agtgcgacgg gcacgcgcag
     2101 tgccagtaac cccagcaata tcgcgagtcc ataaaaacca atttctacag ccatgatctt
     2161 tccttatgct cgactagtta atgggatatt ttggcgatca gcggtatgcg gacaatagac
     2221 accattgccg ccattccaaa tgcaatcggt aaaatgaaat aagttggcca gaccggcatc
     2281 gggaaactga gcgacataac ataggaacct atttcgtact gttcgatcgc cttgctccaa
     2341 gtcgcgatgg tcaatacgac atagatgccc gccgagagca ctgataccag tacatccacc
     2401 acgcggactc cgatgtcacc aaaaaagccg cccagcgcct caacaaatat catctccttg
     2461 cgccattcaa cccaggccaa cggcaacaat gccagagaaa ccatgtagta gcgggtcacg
     2521 agctccaatg ttgctggaat cgacacggac attgtcgagc gaagaatgat gtctaggctg
     2581 atatgcagca tcatggcaac gagacctaaa gtgcccacca aagctaggag tacggcggca
     2641 cgatccatca agcggacaag ccagttcata attgtcacct ttattgttct atcaaagtcg
     2701 tgcaagccaa catattaagt aacgtcaact ggcctgcaat ccatgtagct cggttcagat
     2761 accgtaagtg ctgaaattca cttgatccca aatttccctt tgctgtgctt cagccatttt
     2821 cgtcgcatcg ttattaacgg cttcagcgat cttggcccac ttgtcgacta gctgacggaa
     2881 atgctcgatt ttagcgtcgg cattcttgac tttataacgc tcacttgctt cccttgcagc
     2941 aaaaacgagg tcattttcga caaagactgc agtcgccgta agcagctcct gactgggttc
     3001 aatagcctga caagcatcgc aagtgacaac atactcagtc atggctgctg ccatcgcctg
     3061 aggattgtta cccaaaaatg ctaagtcacc tatcaggtta atctcaggta aatcagccgg
     3121 aaaataagct ggcaggaaca gccct
//
LOCUS       DASRMS010000138        22819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_573543, whole genome shotgun sequence.
ACCESSION   DASRMS010000138 DASRMS010000000
VERSION     DASRMS010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 22819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22819
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573543"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..2073)
                     /locus_tag="VFC74_06290"
     CDS             complement(25..2073)
                     /locus_tag="VFC74_06290"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK24979.1"
                     /translation="MKKLKRSKIRTGKNKFIHSKLYHQEVNALFECVNTVFTNVQFDD
                     AVRKIYDIAKRIIGNTVGFAAELNEERTTNNILFLDSAALPFRVPVNVPLPVDGLHAY
                     AYQTDRIFYENNFATSKWVKDLPASHLPFENLMVIPLTYAEQTAGLLGLVNKPGGFTE
                     HDVVLATIFAEFASLTLSKKHLLEELAASEDKYKSLVEILPEAILIHKQGKIIFTNAI
                     ASELLGVTDGSSLEGKSILRYLLPEDKLLFAKKCIATKKLPINLMERSVIREDKQIIT
                     VETSTANFKYNGDNAEIFVFRDITARKKLQEETLIATKLEAINILAGGIAHDFNNILT
                     VILGNLSMAKMLTASGTEIEDRLKEIETAALQTKELTRQLLTFAKGNPLIKKQASLAE
                     ILQEMITFALRGTNVEPFFNFAEQLPAVAIDIGQFGQVVNNLVINAVQAMPHGGQLYL
                     QATLEHLTNYSGLLAPGDYVKVSFTDTGVGISSLHLPQIFDPFFTTKEQGNGLGLVTS
                     YHIIKQHGGQITVESELGKGSTFHLYLPAAHEKVTPEPEEMTIVHGYGKILLMDDEYG
                     VRKTACEMLNLLGYEPDWAKNGEEAFTRYLRAQQSGTPYDLVILDMTVRGGMGGQEAM
                     EALLQIDPQVKAIIATGYTTAPLITDYKKYGFSGYIYKPFRLEELSSALNKLLTAHKE
                     "
     gene            complement(2294..4054)
                     /gene="recQ"
                     /locus_tag="VFC74_06295"
     CDS             complement(2294..4054)
                     /gene="recQ"
                     /locus_tag="VFC74_06295"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HZK24980.1"
                     /translation="MLTEAQTILQKIFGYPSFRGGQAKVVESLLTRTDTLAIMPTGAG
                     KSLCYQIPALLAEGVTLVISPLIALMKDQVDALGEHGVPATFINSSLKAAELRKRLQD
                     IADGRYQLVYVAPERLAAEGFLELAVNLQISFLAVDEAHCVSQWGHDFRPSFRKIAPF
                     INALPKRPVIGAFTATATPEIKQDIIELLGLVDPQVFVTGFDRQNLHFTVLRGENKQE
                     FVLDYLQANRGKSGIIYAATRKDVDTLTKNLQEHGIAAGKYHAGMRDLERGENQEAFL
                     RDDLLVMVATNAFGMGIDKSNVRFVIHYSIPKNIEAYYQEAGRAGRDGEPGECILLYH
                     PQDVILQKYLIEQTVYSSRRRQNEFSKLQAVTDYCHTSHCLRQTLLEYFGEVDVHDNC
                     DNCSNCKDDREVVDVTTEAQKVLSCVYRMQERYGANLVAQVLKGANNKRIREFRLDRL
                     STYGLMPKQTIQELRDFIGFLTAEGYLRLTEGKFPLVKLGRSAAAVIKGQQQVLRKVK
                     VQEVFVEDGLFAILRQVRKEIADRENIPPYMVFSDATLRQMCEKRPADHAALLRVSGV
                     GEMKLEKYGNDFLDAIKTAN"
     gene            complement(4135..6561)
                     /locus_tag="VFC74_06300"
     CDS             complement(4135..6561)
                     /locus_tag="VFC74_06300"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK24981.1"
                     /translation="MILNTVKLSPFGGINGREVQFKPGLNVVVGPNEAGKSTMVNALF
                     AVLFLPTNLALNSKLWKNNLARFLPYGGGDTMLVTVDFQDDAGHTYSLSRSWGATKSC
                     RLTMADGNVITSETKAQEIMAEILQYGRGTYEGVLFARQEEMMQTIELLRQNNEAAAT
                     LGDILRTALLQGGGVSLEEFAIELSQKKKALLSNWDLERDAPKNNRSVDNPYVQNVGD
                     LLKAFYAKERLRRRLQASTAAEKQVETALIILEQAAAAKAELAPRKAVLEALESDVIK
                     RSSVTPELKTLALQEEKLKVINREWPQETERKNNLQKSIAAAKVRLTNLAAELEAAKA
                     AVAAKKLRERYLTAKPIQEELTEMQHKLTAVPQVTAADVQELEQQQNKLTNKETEVQA
                     MKLLAELSLAKPQTITVTSALAEPQVVEVAGEAAFSGAGSLLFATEEWTLKVQAGQCD
                     VEALMEEITTVKAQMQEKLRQLSLTDLSAVRQNLESRQMLAEQISRLQAKLEGVLQEQ
                     AFAEIAAAVEEITEIMTREPEMVLTELLQVQADLKHAEAQVQAILQRLTAWEQEYGNY
                     DAVMDKVVDIRTEVRQLQTELTYLAPLPEEYADTDSFLSALKSLREQSQQLETQLAAA
                     KEQLLGAQQEQGTDTADDLQARYQEAEQHFQRIKREAAALLKVEVKFNHLSTQLNQNT
                     FAPFAAAFCRYLAPATGYRYQTAALAGTLPCQLEAADGKTLPVELLSTGTTRGIALAL
                     RLAMAEFLLQQSGGFLVMDDPLVDLDPARQKHAASMIRDYAQTRQVIITTCDPDTANR
                     LGGNVIDWNK"
     gene            complement(6558..7667)
                     /locus_tag="VFC74_06305"
     CDS             complement(6558..7667)
                     /locus_tag="VFC74_06305"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HZK24982.1"
                     /translation="MLKVLHTGDLHLGIMHTTRNYPEELRQNLVQARLTTLARLVEQA
                     NQTSCHLFVIAGDLFNSHNVTKELVLETIRILNRFTGACVAVLPGNHDFYTEMITLWQ
                     TFLDQAPEQIILLSQQKPYPLQDFGLDVVLYPAPCDRRHSATNRLGWLAELTEQPAGK
                     WHLGVAHGTLRGISPDLDDSYFPMERDELLRTGLQHWFLGHTHSRYPDLEQTEETGFA
                     YCGTPEPDGFDCSHSGYAWLTTFSDGKVLNQSLATGQYQFAEKSLEIRDLAAVEKFFT
                     EIAAETLLLKLSLTGRLPREEYQQRAGLLKQCATKVAYLEYDDSLLRMELSAASIAAE
                     FPVGSFPHLLLTSLADAGDREALQLAYELVKKVKK"
     gene            complement(8009..8617)
                     /locus_tag="VFC74_06310"
     CDS             complement(8009..8617)
                     /locus_tag="VFC74_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24983.1"
                     /translation="MFTEQEEQILTYVMGLLHYYGAAYCSDLYERVEEQLQVGWSKED
                     FHRLLERTVLNDNHPYVMELEDEICFDFEVDDSEWVLAQQQQQAALDFRPVNEREAAC
                     LLEDRHLLLWSDSEEAFYAWLEARCDDSDLALTVFLEYASLLKNGLTPLALAQKLVQQ
                     LAVMQTEIQEVAELVLKFAAATPMWTLKGWRPNELPLSSLVK"
     gene            8802..9452
                     /locus_tag="VFC74_06315"
     CDS             8802..9452
                     /locus_tag="VFC74_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZK24984.1"
                     /translation="MDTRSEHLERKDFARYEQIALIIARDITRGVYLEGEKISGRTTL
                     ASKYSISPETVRKALALLSAKQVVDVIPNSGTIILSRAAAQLFADDFKEHNALATMER
                     RLTALSKERDQLNLEIERLVKEIVGFKDSMLKNMHNAEEITVGPNSPLIGESVQTAQL
                     RTVTGATVTAIKHHGRWQISPGADIKLAEGDILLVLGSHQSIKYLRKLAAGSKHQA"
     gene            9487..10650
                     /locus_tag="VFC74_06320"
     CDS             9487..10650
                     /locus_tag="VFC74_06320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK24985.1"
                     /translation="MVRFENITKKYNNVAVVNNLNLEIKEGELVTLIGPSGCGKTTSL
                     KMVNRLIDPTSGTIYVNGEDIMRQDPVQLRRGIGYVIQQIGLFPNMTIAQNVAVVSKL
                     QGWNKEKQIARVKELLEMVGMDPASYMARYPKELSGGQQQRIGVLRALAANPKIILMD
                     EPFGALDPITRDQLQGELKKLQAQLRKTIIFVTHDMDEALKLADRIVLMRDGEIVQTA
                     SPDEILRNPADKFVASFLGKDRLLRSPDAVKVEEVMISDPVTIEYNRGLAEALERMRM
                     RHVDGLMVVDDLEHLVGVLSVKNLQADLKKGGTIEKYIVPHPAAVKTGQTVREAVHLM
                     AQYNVGYLPVIDEDNKLRGLMTRSSLVDILTDVMWPGLDAPHELLPATEKEVV"
     gene            10651..11325
                     /locus_tag="VFC74_06325"
     CDS             10651..11325
                     /locus_tag="VFC74_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK24986.1"
                     /translation="MPDRISIFAKVYTFIASDPAQFITVLQEHLLYLVLIPVSLAILV
                     AVPLGILATRYKWIEKIALGVTSMLQTIPSLALLALMIVMGLGIGYKPAITALFLYSL
                     LPILRNTFIGIRNVDPALKEAATGMGMTNMQRLRMVELPLAFSVIMAGIRTATVTCIG
                     TGTLAAYVGAGGLGVFIVRGLQLFRNYMLIAGAVPAAVMALTADFLLGKLEYALIPRG
                     LKNDNQ"
     gene            11542..12900
                     /locus_tag="VFC74_06330"
     CDS             11542..12900
                     /locus_tag="VFC74_06330"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HZK24987.1"
                     /translation="MRKIKLLAVLLLFTLLLVACNKDPAPTLPPQEGEIADLANEFVA
                     KLKNEDFTSAVGFFDTVMKKELPVSRLEEVWKTVEEQVGAFQSITETRTEDMGQAEIA
                     IVTARFAQNLLDIRITFNQDKRISGLYFVPAQNQTTAYKTPPYAHPAKLEEIEVTIGE
                     EWALPGTLTIPQGSGPFPAVVLVHGSGPNDRDETIGINGAVKPFKDLAWGLASQGVAV
                     LRYDKRTKVYPDKLAALSNFTVQEETIADALAAVELLSQQKKIDPKKIYVLGHSLGGM
                     LAPRIGAAAEDKLAGLVILAGPTRPLEELILEQTKYLANADGSTTEQEAASIRQLEAQ
                     IVNIQDPNLSPSTPPTELFNAPASYWLDLRSYNPAETAKNLNLPMLILQGERDYQVTM
                     TDFAKWQQTIGEQSGVQLKSYPGLNHLFIKGEQPSLPAEYEEPGNVAQEVITDIATWL
                     QK"
     gene            complement(12975..13163)
                     /locus_tag="VFC74_06335"
     CDS             complement(12975..13163)
                     /locus_tag="VFC74_06335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24988.1"
                     /translation="MTLIGLLERIAEANKMMFGQHGKRRLTPGSLHEFSSRLQLPYVS
                     VANSLHDEGDGWLENNCS"
     gene            13437..13994
                     /locus_tag="VFC74_06340"
     CDS             13437..13994
                     /locus_tag="VFC74_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005896522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HZK24989.1"
                     /translation="MIMPYLIGAIIVIGLFVMTSYNGLVALRQRVQNAWAQVDVQLRR
                     RYDLIPNLVNTVKGYAKHESETFEKVTAARTQAMAATTVQEQAGAENMLSGTLKSLFA
                     LAENYPQLKADSNFKQLQEELSATENKIAFSRQFYNDTVQKYNTKREVFPTVLIAGML
                     GFHAAEYFNLDSDADARNPVNVKFD"
     gene            14001..15908
                     /locus_tag="VFC74_06345"
     CDS             14001..15908
                     /locus_tag="VFC74_06345"
                     /inference="COORDINATES: protein motif:HMM:NF044955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HZK24990.1"
                     /translation="MPEKRQLSSRCSLLLLIFCLLLIMPQQVWARSYDFPQITIEAEI
                     TPEGDMWVTEQRTVNFNGTFHGLSLWIKTQNPITISNVEVAENNLTYTFNPGTEYGPA
                     GTYMIKDEADRFLIDWSFDATNTNRTFTVRYRVNDVVQVHDDVAELYYQFIGGEWEVA
                     AHQVKVNLHLPEKAAVNEIRAWGHGPLYGEVQIPANDLITWEIEPLPAYTFLEGRVTF
                     PPSLVPLATHSTGKAALPDILAEEEQWADQANREREEAKQEVDNENDWRPLPIIPRQS
                     PLLPYLAAFILLGSSGFAFWAWYRYGKEFKPNFSGDYYRELPADYTPAELGVLWRFGK
                     TTTEDLTATLIDLARKGFLQIEEVVVEKRSIFFGSKKEDYRVIRTAKQASLAEHEEQL
                     LDFLFTRVSKDNAFTFDELEDYAKKHASRFRSFWTDWQNTVSAQSGKLDFFDTSVNLV
                     KGIVIVVGILMLPLGLLMANLLTPAFIIGGIIIVISGFSLNRRSRNGVEQFVRWRAFR
                     KFLLDFSNMESHELPSLVIWEHYMVYAITLGVAKEVIKQLQLVFPLESDGQSFGNGWF
                     MYSGAYRAASFAAMTRSFDKLNQSFNETVRIASSPPSSGSGGGGGFSGGGGFGGGGGG
                     GGFGGGGGSAR"
     gene            complement(16018..16518)
                     /locus_tag="VFC74_06350"
     CDS             complement(16018..16518)
                     /locus_tag="VFC74_06350"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK24991.1"
                     /translation="MKSQTMLLGSLALLLFIIGLRRKDGSHKKGLQLGAKVFYSYLPV
                     LVLAFFLAGLLQVALPTTLVRNWLGAESGWRGIFVGAFSGWLFATGPYAVFPIIASMM
                     HSGASIGTVIALSTSWALLSLSKLPYELAILGPRFTLTLKIVTFLMPLLAGFLAQVLF
                     GSVVII"
     gene            complement(16515..17069)
                     /locus_tag="VFC74_06355"
     CDS             complement(16515..17069)
                     /locus_tag="VFC74_06355"
                     /inference="COORDINATES: protein motif:HMM:NF015715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HZK24992.1"
                     /translation="MKKTIIVMSAAVVVLSMLAYLQGGTALVGAGFLIGVRMMWSVLP
                     MLVAAFALNGLVQVLVNRDKITTILGKSAGVKGMLLGSLGGGLLPGGPYVVYPLISSF
                     MGTGADAATIMALVVGKSLLDLARLPQEAAFLGTKVMAIRYGITFTFPLLAAILTKIF
                     FPKLTEELATFRKQLEKQEEAAKE"
     gene            17240..17806
                     /locus_tag="VFC74_06360"
     CDS             17240..17806
                     /locus_tag="VFC74_06360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24993.1"
                     /translation="MLQHKVKLALLMLALLLLTACGPPNPELTVQDYWQAVVNGNAAE
                     AAAYIQPNEATESWLKKLWPDNSAEFVDDAKTALFLQRITITPTSHEINGSQATVNVT
                     VTWPQLDNFLGNFMTEALSEVFSLALSHAAENEIDAALETIFTAVLVDTPDTHTEHVV
                     KLVLEEGKWKLTSSPLPDPAVLFNLQAK"
     gene            complement(17869..21429)
                     /locus_tag="VFC74_06365"
     CDS             complement(17869..21429)
                     /locus_tag="VFC74_06365"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HZK24994.1"
                     /translation="MARNIFGKTWWSRAWLEALTEIDYRNRLPRGRKYAADGAVTKIE
                     VNDGLVQARVRGRRYIPYKITLHLKPFSSADVTKLADILSKDALLTAELSAGKLPEDL
                     PALLTAAGLSLFPGEWQQIKAACSCPDGANPCKHVAAVFYILANEIDKDPFVLFELRG
                     LKRRDLLKMAGFTNSESQAKAFIPLSEATWSTKVPAEFSVAELASRKNAAATLFTLLQ
                     EEPVFYGEGNFKSILYQAYQGLAQYIDKVEIPKDSLIPQEAFVRLLWNQEQFAHPLEQ
                     ITAVIYNSGHKLTNGASTNRTVPMLEEGKLVLKRIKGRIISLKYLLWLFLQSPLLLEG
                     SSEVVFLSAAASVALVLVQTSLFVPEVVASKEGRFIVRYRPRTEDLEVQAVVEQLALM
                     LPPTLGYRGCDHSVLSGQLAAEEVLALYFACLVQDVDELPAWDKLSWVFFGTDVYLPQ
                     RFEERQTGQSVAAWLTPLSVAAGRFIPVVRVEVPVGRQKKFRVYLEVEDRHQSLSAIV
                     PLADLYEKRAVFNASSGEVLAQVAKQVAVAAEYAPELKELLAQKGSPLLLSGPTLVQF
                     LSAGQNILAMLGIPILLPKEIRSVVASKLVLAAKTTTHAVSYLSLNEILDFSWEVALD
                     EMRLSKDEFLSLATSAEGVVRYRDQYLLLEPAEVTRLLAQLQKPAPKLSSGQVLRAGL
                     TGETDGALFAMDETLQSIMAEIHSVREISTPTGLQGILRPYQERGFRWLYSNWERGLG
                     SCLADDMGLGKTVQVITLLLKLKEDAKLRPPALVVCPTTLLGNWEKECARFAPQLRIA
                     ICHGPDRQLETTKADLILTTYGVVRNDCADFARIRWSLLVIDEAQNIKNADSGQAQAL
                     KQLTAAGFVAMSGTPVENRLDELWSIFDFMLPGFLGSRLNFARSFAVPIEKYRDQTRA
                     ALLRKATSPFVLRRMKTDRSVITDLPDKIIKNEFCRLTPAQTALYQEVLQQEIAEIAD
                     SSGMTRRGRILRLMTWLKQICNHPVHFARKGVVSSAESGKAELAISLLQQIIADGEKA
                     LIFTQYRAMGELLVQMIREEIGLTVSFFHGGLQRKTRQRYIDAFQQKDGCPLLVVSLK
                     AGGTGLNLTEATHVLHYDLWWNPAVEDQATDRAYRIGQDKNVTVHRFISLGTLEEKID
                     AMLTAKKQLAEMTVSAGETWLTEMTNEQLREIFTFQDIER"
     gene            complement(21619..22476)
                     /locus_tag="VFC74_06370"
     CDS             complement(21619..22476)
                     /locus_tag="VFC74_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003374925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HZK24995.1"
                     /translation="MAIQILTDSAADLPLSIIEEYGIDIAPLHIYGDGEEYLDGKDLL
                     SPQMLNAMRQGKVFKTSQVDPATFLEYFKLYAARGDSCIYVGLSSELSSTYQSAVLAQ
                     KEIQKTYPDFTVHTVDTKCVSLGQGLVASKAAQLAKEGKSIPEILASVEFYAKHMEHI
                     FTVDNLEYLYRGGRVSRATALVGGILNIKPVLQVAEGKLVPLEKLRGKNKVFRRLVEL
                     IGEKGVDLDTQTIGISHGDDLENAAKLKKLIQEAYGCTDIIINPIGCVIGAHAGPGTL
                     ALFFLNSKP"
BASE COUNT         6306 a         5410 c         4778 g         6325 t
ORIGIN      
        1 atgtataatt ttactaactt taatctactc tttatgagct gtaaggagtt tgttcagtgc
       61 actacttagc tcttccaaac gaaaaggttt ataaatataa ccactaaaac catacttttt
      121 ataatccgtg attaaaggcg ctgtcgtata gccagtggca atgatcgctt tgacttgggg
      181 gtcaatttgt agtaaagcct ccattgcttc ctgacctccc atgccgccgc gtaccgtcat
      241 atccaaaatt accaggtcgt agggagttcc actttgctgt gcgcgtagat aacgggtgaa
      301 cgcctcttcc ccatttttgg cccagtctgg ttcgtaccct agcaggttta gcatttcaca
      361 tgcagttttg cgcaccccgt actcatcatc catcaataaa attttgccgt aaccatgtac
      421 aattgtcatc tcctcgggct caggggtgac tttttcgtgg gcagctggca aataaagatg
      481 aaaagttgat cctttcccta gttccgattc aactgttatc tgcccgccat gctgcttaat
      541 aatatgataa gaagtaacta gccccagacc gttaccttgt tcttttgtcg taaaaaaggg
      601 atcgaatatt tggggcaaat gcaaactgga aatacctacc cctgtatctg taaaagagac
      661 cttgacatag tcaccaggtg ctaacaaacc agaataatta gttaaatgtt ccagcgtggc
      721 ttgaagataa agttgacctc catgaggcat ggcctgtacg gcgttaatga ctaggttatt
      781 aacaacctga ccgaattgac caatatctat tgctactgct ggcaattgtt ctgcaaagtt
      841 aaaaaacggt tctacattcg tgccacgtaa agcaaaagta atcatttcct gcagtatttc
      901 tgctaacgat gcttgttttt tgatgagagg attgcctttg gcaaaagtaa gcagctggcg
      961 tgtcagctct ttagtttgca gggcagctgt ctcaatttcc tttaatctgt cttctatctc
     1021 tgtaccactg gctgtcaaca ttttagccat cgatagatta ccaagaatta ctgtgaggat
     1081 attattaaaa tcatgcgcaa tgccgcccgc taaaatatta attgcttcca gcttagtggc
     1141 gatcaatgtt tcttcctgca gttttttgcg tgctgtaatg tcgcggaaaa caaaaatttc
     1201 tgcattatca ccattatatt taaagtttgc cgtgctagtt tctacagtta taatctgctt
     1261 gtcttcacgt atcacactac gttccattaa attaattggc aatttctttg tagcgataca
     1321 tttttttgca aataatagtt tatcttctgg taagagatag cgcagaatac ttttgccttc
     1381 taatgaactg ccgtctgtaa ctcccaaaag ttcagacgct atcgcgttag taaagataat
     1441 cttgccctgt ttgtgaatta aaattgcctc aggtagaatc tccacgaggc ttttatactt
     1501 atcctcactt gctgccaatt cttccagcag gtgtttttta gataaggtca gcgaggcaaa
     1561 ttcagcaaag atggtggcta acaccacatc atgttccgta aaaccacccg gtttattaac
     1621 caagccaagc agccccgcag tttgttcagc ataggttaaa ggaataacca ttagattttc
     1681 aaaaggcaga tgactggctg gcaaatcctt tacccactta ctagtagcaa agttattttc
     1741 ataaaagata cgatcggttt gataggcata tgcatgcaga ccatctacag gtaagggcac
     1801 attgactggc acccgaaaag gaagtgctgc agaatcaaga aagagaatat tgtttgttgt
     1861 gcgctcctcg tttaattctg ccgcaaaacc gactgtatta ccaataatcc gtttggcaat
     1921 gtcataaatc ttgcgcaccg catcatcgaa ctggacatta gtaaaaactg tatttacaca
     1981 ttcaaaaaga gcatttactt cctgatggta caacttactg tgaataaatt tattttttcc
     2041 tgtcctaatc ttactccttt ttaatttttt catggagaat cctcctccaa ataagttaag
     2101 gactagctaa tactcaatct ttaattgtta agaaatttca acaaaagtaa acaattccct
     2161 gcctaaatat tctaaagata gtcctgataa ataatataag ctcacggtca ttaagaccgt
     2221 gagcggtttg ataacacttt ctgaagaatg ttttatgcaa gctaatttgc agaactcgtg
     2281 atagtacaaa ttattagtta gcagtcttga tggcgtcaag gaagtcatta ccgtatttct
     2341 ctagtttcat ttcacctaca ccacttacac gtaataaagc ggcatgatcg gcaggacgct
     2401 tttcacacat ctgccgcagt gtagcgtcgg aaaagaccat atagggtggg atgttttcgc
     2461 ggtcagcgat ttctttacgc acctggcgta gtatggcaaa cagaccatcc tccacaaaca
     2521 cctcttgtac ttttactttt cgcaatactt gttgttggcc tttaatcacg gctgcggcac
     2581 tacgacctaa cttcacaagg gggaacttcc cctcggtcaa gcgcaaataa ccttctgcgg
     2641 tgaggaaacc aataaaatct cttagctctt gtattgtctg tttaggcatt aaaccatagg
     2701 tacttaatcg gtctagcctg aattcgcgga ttctcttatt attggcacct ttcagcacct
     2761 gggcgacaag attggcgccg tagcgttctt gcatccgata cacacaggag agcactttct
     2821 gtgcctcagt ggttacatct actacttcgc ggtcatcttt acagttactg caattgtcgc
     2881 agttatcatg aacatcaacc tcaccaaaat actcaagcag tgtctgacgt aggcagtgcg
     2941 aggtatggca ataatcagta accgcctgca gcttactaaa ttcattctgc cgacggcggg
     3001 aagaataaac tgtctgttcg atgagatatt tctgcagaat tacatcctgg ggatgataaa
     3061 gcagaatgca ttctccaggt tccccgtctc gccctgcgcg tcccgcttct tgatagtaag
     3121 cttcaatatt tttaggaatg ctgtaatgga tgacaaaacg tacattagac ttgtcaatgc
     3181 ccataccaaa ggcatttgtt gctaccataa ccagcagatc atcacgcaaa aaagcttctt
     3241 ggttctcccc ccgttctaaa tcacgcatgc cagcatgata tttgcccgcg gcaataccat
     3301 gctcctgcag gttttttgtc agtgtatcta catctttacg cgtggcagca taaatgatgc
     3361 cggatttacc tcggttcgct tgtagataat cgaggacaaa ctcttgcttg ttttctcctc
     3421 gtaagactgt aaaatgtaag ttttgtcgat caaagcctgt gacaaaaact tgtggatcaa
     3481 caagtcccag cagctcgata atatcttgct tgatttccgg tgtagctgtt gctgtaaaag
     3541 ctccaatcac tggtcttttg ggtaaggcat taataaaggg tgcaatttta cggaaactag
     3601 gacgaaaatc atgaccccat tgtgaaacac aatgggcttc atcgacagca agaaaggaaa
     3661 tctgcagatt gactgccagc tccaagaagc cctctgccgc taaacgttcc ggtgccacat
     3721 acaccaattg ataacggcca tcagcgatat cttgcaggcg cttacggagt tctgcagcct
     3781 ttaaggagct gttaataaag gtagccggta caccatgctc gcctaaggca tctacttgat
     3841 ctttcattaa agctatgagc ggtgaaatta ccagtgtcac tccctcagcg aggagggcag
     3901 gaatttgata gcacagcgac tttcctgcac cagttggcat aatggctaaa gtatcagtac
     3961 gcgtgagcag actctccacg accttggcct gtccacccct gaatgacgga taaccaaata
     4021 ttttttgtaa aattgtttgt gcttctgtta gcatacctac cccccactac atatccttga
     4081 aatatatgtc ctgttgtcag ctaaataatt tgtgctgttt tgccaagtct accattactt
     4141 gttccagtca atgacatttc cgcccaacct gttggcggtg tccgggtcac aggtggtgat
     4201 gatcacttgc ctggtttgag cataatctct aatcatgctt gcggcatgtt tttgccgtgc
     4261 cggatccaaa tccactaatg ggtcatccat gactaaaaat cctccacttt gctgcaaaag
     4321 aaattcggcc atcgccagac gtagagccaa agcaatacct cgcgtggtac ctgttgataa
     4381 tagttctaca ggcaaagttt taccgtctgc tgcttctagc tgacagggta aggtgccggc
     4441 taatgctgct gtttgatagc gatatcctgt agcaggtgct aaatagcggc aaaaagccgc
     4501 agcgaaagga gcaaatgtat tttgattaag ttgtgtactt aaatgattaa acttgacttc
     4561 tacctttaac agagcggctg cttctcgctt aatccgctga aaatgttgtt ccgcctcttg
     4621 gtaacgtgct tgtaaatcat cagccgtatc cgtgccttgt tcttgttgtg ctcccagcag
     4681 ctgctcttta gctgcagcaa gctgtgtttc aagctgttga ctttgttcgc gcaatgactt
     4741 taatgccgac aaaaaggagt ccgtgtcagc atattcctca ggcaggggag ctaaataagt
     4801 taattctgtt tgcagctgac gtacttcagt acgtatatct accactttgt ccatgacggc
     4861 atcatagttg ccatattcct gctcccaagc ggtaagacgc tggagtatcg cctgaacttg
     4921 agcttcagca tgctttaaat cggcctgcac ttgcagcagc tctgttaaga ccatttctgg
     4981 ctcacgcgtc attatttctg ttatttcctc cactgcagca gctatttcag caaaggcttg
     5041 ctcttgcagc acaccctcta atttcgcctg caaacggcta atttgctctg ctagcatttg
     5101 gcgcgactct agattttgac gtactgcgga taaatctgtt aaagacagct ggcggagttt
     5161 ctcttgcatc tgtgccttaa ctgtagtaat ttcctccatt agtgcctcca catcgcattg
     5221 gcccgcctgc acttttaatg tccactcctc cgtggcaaac aacaaactgc cagcaccgct
     5281 aaaagcagct tcacctgcca cttccacaac ttgtggttca gctaacgccg aagtcacagt
     5341 aatagtctgt ggtttggcca gtgagagctc tgccaagagt ttcattgctt gcacttccgt
     5401 ttccttatta gtaagtttat tttgctgctg ttccaactct tgcacatcgg cagctgttac
     5461 ttgcggtacg gcagtgagct tatgctgcat ttctgtcagt tcttcttgaa ttggttttgc
     5521 tgttaaataa cgttcacgca gctttttagc agccacagca gctttagcgg cttccagttc
     5581 agccgctaaa ttagtcaggc gcactttcgc tgccgcaata gatttttgta aattgttttt
     5641 acgctccgtc tcttgcggcc actcccggtt gataactttt aacttctctt cttgtaacgc
     5701 tagcgtcttt aattccggtg ttacgctgct gcgcttgata acatcacttt ctaaagcttc
     5761 taaaacagct tttcgaggtg ccaattcggc tttagctgca gcggcctgct ctaaaataat
     5821 taaggctgtt tctacctgtt tttcagccgc tgtgcttgct tgcagtcggc gacgtaagcg
     5881 ttctttagcg tagaaggctt tcagtaaatc acccacattt tgcacgtagg ggttgtccac
     5941 actacggttg tttttaggtg cgtcgcgctc tagatcccaa ttactcaaga gtgctttctt
     6001 tttttgtgac agctcgatgg caaactcctc cagcgacacc ccgcccccct gcagcagagc
     6061 agtgcgcaaa atatcaccta aagttgcggc agcttcgtta ttttggcgaa gcagctcgat
     6121 cgtttgcatc atttcttcct gacgcgcaaa caacacccct tcataggtgc cgcgaccata
     6181 ctgcaaaatt tctgccatta tttcttgtgc cttggtttca ctagtaatca cgttaccgtc
     6241 ggccattgtt aaacgacaac tttttgtagc accccaggaa cggcttaagg aataagtatg
     6301 accagcatca tcttgaaaat caactgttac cagcatcgta tcaccgccgc catagggcag
     6361 aaaacgagct aaattgtttt tccacagctt tgaattcaga gctaaattag taggtaaaaa
     6421 taaaacggca aaaagcgcat tgaccatcgt acttttgcct gcttcattgg ggcccacgac
     6481 tacgtttaag cccggcttaa actgaacttc tcggccatta ataccgccaa acggagatag
     6541 tttaacggta ttaaggatca ttttttcacc ttctttacca gctcataggc tagttgtaaa
     6601 gcttcccggt caccagcatc agccaaagaa gttagcaaga gatggggaaa agagcctact
     6661 ggaaattctg cagcgatact agctgcagat aactccatcc ttaataagct gtcatcgtat
     6721 tccaaataag cgactttagt ggcgcattgc ttgagtaggc cagcacgctg ttgatattct
     6781 tcccgcggca ggcgacctgt tagagatagt ttcagcagta aagtttcagc cgcaatttct
     6841 gtaaaaaact tttccactgc agctaaatca cgaatttcta atgatttttc tgcaaactga
     6901 tactgtcctg tggccaaaga ctgatttaga actttaccat cactaaatgt agttagccag
     6961 gcatagcccg agtggctgca atcaaaacca tcaggttcag gtgtgccaca gtaggcaaag
     7021 ccagtctctt ctgtctgctc taaatcaggg taccggctat gagtatgacc aagaaaccaa
     7081 tgctggagac ctgtacgcag caattcatct cgctccatgg gaaaatagct atcatcaagg
     7141 tctggcgaaa taccacggag cgttccatgt gctaccccta aatgccactt accagcaggt
     7201 tgctctgtca gttcagcaag ccagcccaaa cggtttgtgg cgctatgcct gcgatcacat
     7261 ggtgctggat agaggactac atctaaacca aaatcctgca gggggtaagg tttttgctgg
     7321 gaaagtaaaa taatctgttc cggtgcctga tctaaaaagg tttgccataa tgtgatcatt
     7381 tcagtataaa agtcatggtt gcctggcaaa accgctacgc aagctccagt aaacctattt
     7441 aagatacgaa tagtctctaa aactaattct ttggttacat tgtggctgtt gaacaaatca
     7501 ccggcaatga caaataggtg gcaggacgtt tgattggcct gctccactag tctggcaagc
     7561 gtagttaaac gcgcctgtac taaattttgc cgcagttctt cgggatagtt ccgcgtcgta
     7621 tgcatgatac ctaaatgtaa atctcctgta tgcagaactt ttaacaagag atcacctcca
     7681 ttatgaaact tgtttctcac aggttacatt agctataaag ctgcttgttt ccttccaaca
     7741 gcacaagaaa gacgcttcct gtttttttaa atgctgcgtc tctcttgtgc tgttggggta
     7801 ggcctaaccc agtgagcggc agcaaacgca gtttaagtaa ccaagcctgc taatgaaatt
     7861 taatagtact attcgctgat acttttggca ttcccctgaa aaaaccccag cggcaagaaa
     7921 agaaagcaat tcaagattga tataatttcc tatctaatcc aaaacagcaa aagcgtgtcc
     7981 cggcagctta cctgacacgc ttttagtatc actttactaa agaagatagc ggcaattcat
     8041 tagggcgcca gccttttagc gtccacatcg gtgtcgcggc agcaaatttt aagacaagtt
     8101 ccgctacttc ctgtatttct gtttgcatca ctgccagctg ctgaactagc ttttgcgcta
     8161 aggctaacgg agtcaaacca ttttttagca gtgacgcata ttccaaaaaa acagtcaggg
     8221 ctagatcact atcgtcacaa cgagcttcga gccaagcata aaaagcctcc tcactgtcag
     8281 accacaacag taaatgccga tcctcgagca ggcacgctgc ttcacgttca tttaccggtc
     8341 ggaagtcaag ggcggcctgt tgctgttgtt gggctaagac ccactcgcta tcatcaactt
     8401 caaaatcaaa acagatttca tcctcaagct ccatgacata gggatgatta tcgtttaaca
     8461 ctgttcgctc cagcaaccgg tgaaagtcct ctttactcca gccaacctgt aactgctcct
     8521 ccactctttc atataaatct gagcaataag ctgcaccgta atagtgcagc agtcccatca
     8581 cataagttaa tatttgctct tcttgttcag taaacatcga aattccctcc ccgttaccta
     8641 ctatttttat atacaaccag cgagcctaaa attcctgctg tgccgctgct tttgctttac
     8701 ctcggcgctt gcatttattt actattatgc atcattatgg tcttgacaaa ccgccaacct
     8761 gtgtaataaa atgagattgt cggtaaaagg aggagctcaa attggacaca cgtagcgagc
     8821 atttagagag aaaagatttt gcacgttatg agcaaatcgc cttgattatt gcccgcgata
     8881 tcactagagg tgtttacctt gaaggagaaa aaatttctgg acgtacgaca cttgccagca
     8941 aatatagtat ttcgccggag actgtgcgta aagcactcgc actattaagc gccaaacaag
     9001 ttgtcgatgt aattcctaat agcggcacta ttattctttc ccgggccgca gcgcagcttt
     9061 ttgctgacga ttttaaggag cacaatgcgt tggccactat ggaacgccgt ctgactgctc
     9121 tcagcaaaga gcgtgatcaa cttaatcttg aaatcgaaag attagttaaa gaaatcgttg
     9181 gttttaaaga cagtatgctg aaaaatatgc ataatgctga ggagattacg gtgggtccaa
     9241 actcaccact catcggggag agcgtgcaaa ccgcacaact gcgcactgtt acgggagcaa
     9301 cggtaacagc aattaaacat catggacgct ggcagatctc tcccggtgca gatatcaaat
     9361 tagctgaagg agatatcctg ttagtcttgg gttcacatca gtcaataaaa tatttgagaa
     9421 aattagctgc cgggagtaaa catcaagcat aagacctaat tctatcaaga gaggtaggga
     9481 aagctgatgg ttcgttttga aaatattaca aaaaaataca acaatgtggc tgttgttaac
     9541 aaccttaact tggagatcaa ggaaggtgaa ttagttacct taattggacc ctctggttgt
     9601 ggcaaaacaa catcgctaaa aatggttaac cgtttaattg accctacaag cggtactatt
     9661 tatgtcaacg gtgaagacat tatgcgtcaa gatccggtgc agctgcgtcg cggtattggt
     9721 tatgtcattc aacaaattgg tctttttccc aacatgacaa ttgcccaaaa tgttgccgtg
     9781 gtcagtaaac tccaaggctg gaataaagaa aaacagatag ctcgggtaaa agagctgtta
     9841 gagatggtgg gcatggaccc tgctagctat atggcaaggt atcccaaaga attatctggc
     9901 ggccagcagc aacgaattgg tgtcttgcgt gcgttggccg ccaaccctaa gattatttta
     9961 atggatgaac cctttggcgc cctggatccg atcacacgag atcaattaca gggagaacta
    10021 aaaaaattac aagcccaact gcgtaaaacc attatttttg tgacccacga tatggatgag
    10081 gcacttaaac ttgctgatcg tattgttctg atgcgtgatg gcgaaattgt gcaaacagca
    10141 tcacctgatg aaattctacg gaacccagct gacaaattcg tagctagttt cctggggaaa
    10201 gatcgactgc tgcgttcacc agatgcagtg aaagtagaag aagtaatgat ctctgatcct
    10261 gttactattg agtataatcg tggtttagct gaagcattag aacgcatgcg tatgcgtcat
    10321 gttgatggct tgatggttgt agacgatttg gaacatctgg ttggcgtgct gtctgtaaaa
    10381 aatttacagg ctgatttaaa aaagggcggg actatcgaga agtatattgt acctcaccca
    10441 gctgctgtaa aaacagggca aactgtgcgt gaagctgtac atttgatggc gcaatacaat
    10501 gtcggctacc tgccagtcat tgatgaggat aataaattgc gcggcctcat gactagatcc
    10561 agtttagttg atattttaac agatgtaatg tggccaggat tggatgcacc ccatgagctg
    10621 ctgcctgcta cggaaaagga ggtcgtttag atgccagatc gtatttctat ctttgccaaa
    10681 gtatatacat ttatcgctag cgaccccgca caattcatca ccgtcctgca ggaacatctt
    10741 ctctatttgg tgttaatccc agtctccctc gccatcttag tcgccgtacc cctcggcatt
    10801 ttagccacac gctataaatg gatagaaaag atagctttag gtgttaccag tatgctgcaa
    10861 accatcccta gtttggcttt gctggcactg atgatcgtca tgggactggg cattggttat
    10921 aagccagcca ttactgcgct tttcctttat tcactgctgc ctattttgcg caacaccttt
    10981 atcggcatta ggaatgttga tccggcgctc aaggaagctg ccacaggcat gggcatgacc
    11041 aatatgcagc gtttgcggat ggtggaacta ccacttgcct ttagtgttat tatggcaggt
    11101 atccgtacag ctaccgttac ctgcatcggt acaggtaccc tcgctgccta tgtcggagct
    11161 ggtgggttag gggtctttat tgtccgtggc ttacagctat tccgcaacta tatgttgatt
    11221 gccggtgccg taccggcagc cgtaatggca ttaactgcag attttctgct gggtaaactt
    11281 gaatatgcat taatcccccg cggtcttaaa aacgataacc aataaattta acgacgtctt
    11341 tagatgagga gctatgccca cggctagctc ctcttatttt tagcacagca tcacacaaaa
    11401 catttagtac atacatagga gggcaatgct taccggcaaa taagggcaaa acgttacttg
    11461 gtacataatt ctagctatgc aggtaaaggc ttgaaaataa agaatataag attagcgtag
    11521 tttaggaaag ggtgaaggat agtgaggaag ataaaattat tagcagtcct cttactattt
    11581 actttattgc ttgtagcctg taacaaagat ccagcgccga ccttgccgcc acaagaaggt
    11641 gaaattgccg atttagcaaa tgaatttgta gctaaactaa aaaacgagga tttcaccagc
    11701 gccgtcggct tttttgatac cgtcatgaaa aaggaattac ccgtaagtag actggaagaa
    11761 gtctggaaaa cagtagaaga acaagttgga gcttttcagt ccatcactga gacccgcact
    11821 gaagatatgg gacaagccga aattgcgatt gtcactgctc gctttgcaca aaacctactt
    11881 gatatccgta taacttttaa ccaagataaa cgtattagcg gactttactt tgtcccagca
    11941 caaaatcaaa ctacagccta taaaacaccg ccctatgctc accctgccaa gctggaggaa
    12001 atagaggtca cgattggtga ggagtgggct cttcccggca cccttaccat acctcaaggc
    12061 agcgggcctt tcccggctgt cgtgctggta cacggttcag gccctaatga ccgagatgaa
    12121 actatcggta tcaatggtgc agtcaagccc ttcaaagatt tagcctgggg gttggctagc
    12181 caaggcgttg ccgtgttacg ctatgacaaa cgtacgaaag tttatcctga taaattagca
    12241 gcactcagca acttcacggt gcaagaagaa accattgctg atgccttggc cgccgtggaa
    12301 ttgctctctc agcagaaaaa gattgaccca aaaaagatat atgtacttgg gcatagttta
    12361 ggcggtatgt tagcaccacg aatcggtgct gctgccgagg ataagctcgc tggtctcgtc
    12421 atcctggctg gccccacccg cccgttagag gaactaattt tagagcagac taaatatctc
    12481 gccaatgctg acggcagtac cacagagcag gaggctgcca gcatccgcca gcttgaagca
    12541 caaattgtaa acattcagga ccccaacctt tctccttcca ctccccctac tgagctcttc
    12601 aatgcccccg ccagttactg gttagacttg cgcagctaca atccagctga aacagccaaa
    12661 aatctgaatt tacccatgct tattttgcaa ggagaacgtg actaccaagt taccatgact
    12721 gattttgcta aatggcagca aactatcggt gaacaaagtg gggtacaact taaaagctat
    12781 cccggtctta atcatctgtt tatcaaaggt gagcagccga gcttgcctgc ggaatatgaa
    12841 gagcctggca acgttgcaca agaagtgatt actgatattg ccacctggtt gcagaaataa
    12901 agagagtgac taagcaaagc caccaaacaa ttttggcggc tttgcctaat tcgcccacaa
    12961 ttattagtaa cattttaact acagttattt tctaaccatc catccccttc atcatgtagg
    13021 ctatttgcta cggagacgta aggcagctgc agccttgaac taaactcatg aagactgccg
    13081 ggagtcagac gcctcttccc atgctgccca aacatcatct tattagcctc ggcgatacgc
    13141 tccaacagtc ctattaaagt caatcaactc atctccttcg tcatcccttt cttactttta
    13201 gtacaaggct ctcgatcctt acaccaccca gcggctctca gcgctaccac agtaaaaaag
    13261 cattacgtgt cctctcaggg gtgtagtcaa aatattacca caattaaggc cggtaattat
    13321 taatatttta gtacgataaa gtaaaactac taatttattt tatacttgcg tatattcttg
    13381 caggagtaaa ccgtacgcta agttaatatg ttaatataaa tctaattttg ggagtgatga
    13441 tcatgccata tctaatcggc gccattatcg ttatcggact atttgtgatg actagctata
    13501 atggcctggt tgccctgcgg cagcgcgtac aaaacgcctg ggctcaagtc gacgtccaat
    13561 tacgccgacg ctacgattta atacctaacc tcgtgaatac tgtcaaaggc tacgcaaaac
    13621 atgaaagcga aacattcgag aaggttacag cagctcgcac ccaagctatg gccgcaacga
    13681 ccgtccagga gcaggccgga gcagaaaaca tgttgagcgg cacactgaag tcactttttg
    13741 cgctggcaga aaactacccg caattaaaag cagatagcaa cttcaaacag ctgcaagagg
    13801 agttaagtgc tactgaaaat aaaatagctt tttcccgcca gttctataac gacactgtac
    13861 aaaaatacaa tacaaaacgt gaagtgttcc ccacagtact gattgctggt atgcttggct
    13921 ttcatgctgc ggaatacttt aatttagaca gtgacgcgga tgctcgtaat ccggtcaatg
    13981 tcaaatttga ctgatgcgct atgcctgaga aacgtcaatt gtcatctcgt tgttcactgc
    14041 tgctgctgat tttttgtctg cttttaataa tgccgcagca ggtttgggca cgatcatatg
    14101 attttccgca aattactatt gaggcggaga taacaccgga aggtgatatg tgggtaactg
    14161 aacaacgcac ggtcaacttt aatggtactt ttcacggact ctcgctttgg ataaaaacac
    14221 aaaaccctat taccattagt aatgtggaag tagccgaaaa taatcttact tatactttta
    14281 acccgggcac tgaatatggc cctgctggta cttacatgat taaggacgaa gcagacaggt
    14341 ttctcattga ctggagtttt gatgccacca atacaaaccg tacttttacg gtgcgttatc
    14401 gggttaatga tgtagtgcaa gttcatgatg atgttgccga gctgtattac caatttattg
    14461 gtggtgaatg ggaagtagcc gcccatcaag taaaggtaaa cctacattta ccggaaaaag
    14521 ctgcggtgaa tgaaatacga gcctggggac atggtccact gtacggtgag gtgcaaatac
    14581 ctgcaaatga ccttattacc tgggaaatcg agcctttacc ggcttatacc tttttagaag
    14641 gacgcgttac ctttcctcct tcactagttc ccctagctac ccattctacc ggcaaagctg
    14701 ctctcccgga tattttagct gaagaagaac agtgggccga ccaagccaac cgggaacgcg
    14761 aagaggccaa acaagaagtc gacaatgaaa atgattggcg tccactgcca atcattcctc
    14821 ggcagtcacc cttgctgccc tacctagctg cctttatttt gcttggcagc agcggctttg
    14881 ctttctgggc ttggtaccgt tacgggaaag agttcaagcc aaatttctct ggcgattatt
    14941 accgtgaact gccagcagat tatacaccag ctgaactagg tgtactgtgg cgcttcggta
    15001 aaactactac cgaagattta acggcaacat taatagattt ggcacgcaaa gggttcctgc
    15061 aaattgaaga ggtggttgtg gaaaaacgca gcattttctt tggcagcaaa aaagaagact
    15121 accgagtcat acgcacagct aaacaagctt ctttagccga acacgaagag cagctgttgg
    15181 actttctctt cacgcgggtc agtaaagata atgcttttac ctttgacgag ttggaggact
    15241 atgccaaaaa acacgcctcc cgcttccgct ctttttggac agattggcag aatactgtct
    15301 ctgcccaaag cgggaaatta gactttttcg atacaagcgt taatctagtc aaaggcattg
    15361 tgatcgtcgt aggaattttg atgctgcctt taggtctact gatggctaac ttactcacac
    15421 cagctttcat cattggtggg ataattatcg tcatctctgg tttttctcta aacagacgtt
    15481 cacgcaatgg tgtagagcaa tttgtgcgtt ggcgtgcctt ccggaagttt ttattggact
    15541 tctccaatat ggaaagccat gagctccctt cactagttat ctgggaacac tacatggtct
    15601 atgccattac actcggcgta gctaaagaag taatcaaaca attgcagcta gtcttcccct
    15661 tggaaagtga cggccaatcc tttggcaatg gctggttcat gtatagtggt gcttaccggg
    15721 cggcttcttt tgctgccatg acacgcagtt ttgataaact caatcagtcc tttaacgaaa
    15781 cagtgcgcat tgcttccagc cccccttctt ctggctccgg cggcggtggt ggtttttccg
    15841 gcggcggtgg cttcggcggc ggtggtggtg gcggcggttt cggcggcggc ggcggcagcg
    15901 cccgctaact atgcctaaac aggcaagaag aaccacgcag ggaggtcgcg tggttcttct
    15961 gctaagcgct aaagttcttt tattatggac agaactctcc cgttaaagtt ggggatctta
    16021 gataataacc acgctgccga aaagcacctg tgctaggaac cctgccaata gaggcattaa
    16081 aaaggtcaca atttttaagg tcaaggtaaa gcgaggccct aaaatagcta actcataagg
    16141 cagcttcgat aaactaagca gtgcccagct agtagataaa gcaattactg taccaatgct
    16201 ggcaccgcta tgcatcatgg aagcaatgat cggaaacacg gcatatggac ctgtggcaaa
    16261 caaccaaccg gaaaatgcac ccacaaagat accacgccag cctgactctg cccctagcca
    16321 attacgtact aaagtagtgg gcaaagcaac ttgtaataac ccagcgagaa agaaagcaag
    16381 aactaacacc ggcaggtagc tgtagaacac tttcgctccc aactgcaaac cctttttatg
    16441 gctgccatct ttacggcgta aaccgataat aaacaggagc aaggctaaac tacccaaaag
    16501 catggtttgt gatttcactc cttagccgcc tcctcctgct tttcaagttg cttacgaaaa
    16561 gtagcaagct cctctgttag cttgggaaag aagattttcg ttaagattgc tgccagcaga
    16621 ggaaaagtga aagtaatgcc atagcgaata gccataacct tcgtacctag gaaagctgct
    16681 tcctgcggga ggcgcgccag atctaaaaga cttttaccaa ctaccagcgc cataatggtt
    16741 gccgcatcag cgccggttcc cataaaggat gaaattaggg gataaacaac gtagggaccg
    16801 cctggcaata aacctccacc caaagaaccc agcagcatac ctttaacacc agcgcttttg
    16861 cctaagatag tagtgatttt atcgcggttt actaaaacct gaaccaagcc attcagcgca
    16921 aaggctgcca ccagcattgg caagacactc cacatcatgc gcacaccaat caaaaaacct
    16981 gcgccaacta aagcagtgcc tccttgcagg taggcaagca tggataaaac gaccacagct
    17041 gccgacatga cgataatggt ttttttcaaa ctatcccttc tttctttgac tcctctacct
    17101 ttaaactaac aaagattatc tgaatagtca aacatttagt agctaaattt ctggctgctg
    17161 ctttccgaaa gcaaaatctc cagcaggaga tcggctagac aatgtcaaaa tgtaactcta
    17221 ccagacgagg agtgatacga tgttacagca taaagttaag ctggctttac taatgctcgc
    17281 cttgctgctg cttaccgcat gcggcccgcc gaaccctgag ttaacggtac aagactattg
    17341 gcaggcagtt gttaatggta atgctgctga agcagccgca tatattcaac ccaacgaagc
    17401 tacagaaagt tggcttaaaa agctatggcc tgataatagt gctgagtttg ttgatgatgc
    17461 caagaccgcc ttgtttttgc agcgaattac tattaccccc actagccacg aaattaatgg
    17521 cagccaagct actgtcaacg ttacggtaac ctggccgcag ctagataact ttctgggcaa
    17581 tttcatgact gaagcgttaa gtgaagtctt ttctttagct cttagtcatg ctgcagaaaa
    17641 cgaaattgac gccgcacttg aaactatttt cacggcagtt ttagtcgaca cacctgatac
    17701 acatactgaa cacgttgtga agctagtttt agaagaagga aaatggaaat taacctcttc
    17761 accattaccc gatcccgcgg tactttttaa tttacaagct aagtagtgac ccacagaatt
    17821 taaaactcgc tcccacgaga gagcgagttt ttttgtgccc caaaaaagtt atctttcaat
    17881 gtcctggaag gtaaagattt ctcgcagctg ttcgttggtc atttctgtaa gccaggtttc
    17941 gccagccgaa acggtcattt ctgccagttg ctttttagct gttaacattg catcgatctt
    18001 ttcttctaaa gtacccaagc tgataaacct gtgtacagta acgtttttat cctgcccgat
    18061 ccggtaggct cgatcggtag cctgatcttc tacagctggg ttccaccata agtcgtaatg
    18121 caaaacatgt gttgcttcgg tcagatttag acctgttcca cctgctttta aggataccac
    18181 cagtaaggga cagccatctt tttgttggaa agcatcaata taacgttggc gtgtttttct
    18241 ttgcaaaccc ccatgaaaaa aggaaacggt caggcctatt tcttctctaa tcatttgtac
    18301 cagcagttct cccatggcac gatattgcgt aaaaatcaag gccttctctc catctgcaat
    18361 aatttgctgc agcagtgaaa tagccaactc cgccttacca gattctgcag aactcactac
    18421 acctttacgg gcaaaatgga ctgggtggtt gcaaatctgc tttaaccaag tcattaaacg
    18481 taaaatccgc cctcttctgg tcatcccaga gctgtcagca atctctgcta tctcttgctg
    18541 cagcacctct tgataaaggg ctgtttgtgc tggggttaaa cggcaaaact catttttaat
    18601 aattttatct ggtaagtcag taatcacgga ccggtcagtt ttcatgcgcc gcaaaacaaa
    18661 cggcgacgtt gctttgcgca acagggctgc tcgtgtctgg tcacggtatt tttcaatggg
    18721 gacagcaaag gaacgcgcaa agtttaaccg ggaacctaaa aatcctggca gcataaaatc
    18781 aaaaatactc cacagttcat caagccggtt ctctaccggc gtaccactca tagcgacaaa
    18841 cccggccgca gttaattgct tgagtgcttg agcctgacca ctatcggcat ttttgatatt
    18901 ctgtgcttca tcaatgacta acaaactcca acgaatacga gcaaaatctg cacaatcatt
    18961 acgcacaacc ccgtaggtag taagaatgag gtccgctttt gttgtttcta actgccggtc
    19021 tggaccatga caaatggcga tacgtagttg cggggcaaaa cgagcacatt ctttttccca
    19081 gtttcctaaa agtgtggtag gacaaaccac tagcgccggt ggtctaagtt tagcgtcctc
    19141 ctttagttta agcaggagag taatcacttg cacagttttg cccaaaccca tatcatcagc
    19201 tagacagctg ccgagcccac gctcccagtt gctatataac caacggaaac cccgttcttg
    19261 gtagggacgt aatattccct gcaagccagt gggcgttgag atttcacgga cagagtggat
    19321 ttctgccatt atactttgta atgtttcatc catggcaaaa agagctccat ctgtctctcc
    19381 cgttaagccg gctcgtaata cttgcccact gcttaatttc ggcgccggct tttgtaattg
    19441 tgctaacaaa cgtgtcactt ctgccggctc taaaagcagg tattgatccc ggtaacgcac
    19501 aaccccttca gcacttgttg ctaaagagag aaattcgtct ttacttaaac gcatttcatc
    19561 gagggcaacc tcccaggaga aatcaagtat ttcatttagc gacaggtagg agacggcatg
    19621 tgttgtggtt ttggcggcta agaccaactt ggatgcgacc acagaacgaa tttctttcgg
    19681 cagcaaaatg gggatgccca acatcgctaa aatattttgt cctgcgctta aaaattgcac
    19741 taaggttggg ccgctcaata gcagcgggct acctttttgt gctaagagtt cttttaactc
    19801 cggcgcatat tcagcagcaa ctgctacctg tttagctacc tgtgccaaga cttccccact
    19861 actagcatta aatacagctc ttttttcata caaatccgct agcggcacta ttgccgataa
    19921 agattgatga cgatcttcca cttctaaata aacgcggaat tttttctgcc tgccaactgg
    19981 cacttctact cttaccacag gaataaaacg gcctgcggct actgataaag gagttaacca
    20041 agcggctaca gactgacctg tttgtctttc ctcaaatcgt tgtggcagat aaacgtctgt
    20101 cccaaaaaat acccacgaca gcttatccca agcaggtaat tcatcaacat cctgcactaa
    20161 acaggcaaaa tataaagcca agacttcttc tgcggccaac tggccgctta acaccgaatg
    20221 atcgcatccc cggtacccta aagtaggtgg cagcattaat gccaactgct ccactaccgc
    20281 ctgtacctcg agatcttctg tacgcggccg atagcgtaca ataaaacgac cttccttgga
    20341 agcgacaacc tcagggacaa atagtgatgt ttgcactaaa actaaggcca ccgaggcagc
    20401 tgcacttaaa aatactactt ctgaggaacc ctctagtaac aatggtgatt gtaaaaatag
    20461 ccataataaa tattttaggg aaattattcg accctttatc ctttttaaaa ctaactttcc
    20521 ctcctctagc atgggcacag tgcgatttgt actagcacca tttgttagtt tatggccact
    20581 attataaatt accgcagtaa tctgctctaa aggatgggca aactgctcct gattccataa
    20641 caggcgcaca aaggcctcct gtggtatcag cgagtctttt ggtatctcta ccttatcaat
    20701 atactgtgct aaaccttggt aagcctggta aaggatgctt ttaaaattcc cctcaccata
    20761 gaatactggc tcctcctgca aaagtgtaaa taaggttgcc gctgcatttt tacgactggc
    20821 aagttcagcc acagaaaact cggctggtac tttagtagac catgttgctt cacttaaagg
    20881 aatgaatgct ttagcttgac tttcactatt agtgaagcca gccatcttca gcaagtccct
    20941 gcgtttgagg ccgcgcagtt caaataaaac gaagggatct ttatcaattt cattagccaa
    21001 aatatagaag acagcggcca catgcttaca gggattagcc ccatcgggac acgaacatgc
    21061 tgcttttatt tgctgccact cccctgggaa aagacttagc ccagctgctg ttaagagggc
    21121 aggtaagtct tcaggtagtt tacccgcgga caattcagcc gtaagcagcg catctttact
    21181 taaaatatct gccagtttag tcacatctgc agagctaaat ggttttaggt gcaaagtaat
    21241 tttatatggt atataacggc gccctcgaac acgcgcctgt actaaaccgt cattaacctc
    21301 aattttagta acagcaccgt cagcagcata ctttctgcct cgtggtagac ggttacgata
    21361 atcaatttca gttagggcct caagccaagc ccgggaccac catgttttac caaaaatatt
    21421 tcttgccatc tccgactcct ccaacccagc agcaaatact gcctcccgct cactttcgta
    21481 tctaactgct aattatatca tataccctgc actttaccca atttacacag acaaaaatag
    21541 gaatggtcac ccattccctg cagcacagct aactattcgt ttttaaaaat cgccaacgca
    21601 gagcattggc gattgtaatt acggtttgct gttaagaaag aaaagtgcta aagtccccgg
    21661 gccggcatgg gcaccgatca cacaaccaat cgggttaata ataatatcag tgcagccata
    21721 ggcttcttga attagttttt ttaatttagc tgcattttcc agatcatcac catgactaat
    21781 gccaatggtt tgagtgtcta aatcaacacc tttttcacca atcagctcta ccaggcggcg
    21841 aaagacttta tttttgccgc gtaacttttc caaaggaacg agtttacctt cagcaacttg
    21901 taggactggc ttgatgttca aaatcccgcc aactagtgct gtagcacgac taactctgcc
    21961 cccacgataa agatactcta aattatccac ggtaaagata tgctccatat gtttggcgta
    22021 aaactccacg cttgctaaaa tttcaggtat tgacttacct tccttagcta attgagctgc
    22081 tttagatgca actaaaccct gccccagcga aacgcatttt gtatccacgg tgtgcactgt
    22141 aaaatcaggg tatgtttttt ggatttcctt ctgtgctaat actgctgact gataagtaga
    22201 agacaattct gaactaagac caacataaat acaactatcc ccgcgtgctg cgtaaagctt
    22261 aaaatactcc aggaatgtag ccggatcaac ttgagaagtt ttaaatactt tcccctggcg
    22321 catcgcgttc agcatttgcg gcgacagcaa atccttacca tcaagatatt cctctccgtc
    22381 accgtaaata tgcaggggcg ctatgtctat gccatactcc tcaatgatgc ttaaaggtaa
    22441 atctgcggca ctatctgtta gtatctgaat tgccattacg tcaccccctt accatgaaga
    22501 cttgttttaa ttatatgcta cttttactga tttttgtacc cctaagtgcc gttaaaatga
    22561 ttacctcaac aacaaagacg cacttcaacc ccaccttcct tttcatttat taagaagctg
    22621 gaacaagaaa ggctgcttcg aattcgaagc agccttgatc ctttgaagca tttatgatct
    22681 ctttacttta catgcggcta cgatctatga ggttggtata tttttcatag gctgcacgca
    22741 ttaatggctc aacacgcgca tctactggcg ggtattcctt cttaaagtcg tcaaagctga
    22801 ttatatattt agaattaaa
//
LOCUS       DASRMS010000139         1435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_169749, whole genome shotgun sequence.
ACCESSION   DASRMS010000139 DASRMS010000000
VERSION     DASRMS010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169749"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VFC74_06375"
     CDS             <1..260
                     /locus_tag="VFC74_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24996.1"
                     /translation="PQDLACGVVNMVFQSIGTMAVLAARLKKVNNIVFVGSVVHIQKG
                     QEALEQFMELYNANIVFPEKARFSTAIGAALSTDYIKIQAK"
     gene            525..>1435
                     /locus_tag="VFC74_06380"
     rRNA            525..>1435
                     /locus_tag="VFC74_06380"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          455 a          273 c          379 g          328 t
ORIGIN      
        1 ttcctcaaga tctcgcttgt ggagtagtta atatggtttt tcaatctatt ggtaccatgg
       61 cagttcttgc agctagatta aaaaaagtta ataacattgt ttttgtagga tcggttgttc
      121 atattcaaaa aggtcaagaa gctttagagc agttcatgga gctatacaac gcaaatatag
      181 tttttcctga aaaagcaaga ttttcaactg caatcggtgc tgctttaagt acggactata
      241 taaaaataca agcaaagtaa ataatacaaa accgtataac atactctcct acggctaaac
      301 gcccggcgat gttgttagaa gaccgaataa agttagaaaa gaatcagaaa attggttgac
      361 aataaaaaaa gtgtttgcta ttattgtcaa gcactcaaaa aacgggtgcg accaacggac
      421 cttgaaaatt gaacagcaga caaaacatga gacggactca agttaataaa ttgagcaaag
      481 cgaatgtaca aagtaataag agctagacgc tctcattata taataaaaat gagagtttga
      541 tcctggctca ggatgaacgc tggcggcatg cctaacacat gcaagtcgaa cggagttacc
      601 ggagtggaag acttaggttt gaagcactga ctaacttagt ggcggacggg tgagtaacac
      661 gtgagcaacc aaccttttac acggggataa cctggggaaa ccctggctaa tacccgatat
      721 tatcaagaga tggcatcatc acttgatgaa agatttatcg gtaaaagacg ggctcgcgtc
      781 cgattagtta gttggtaagg taatggctta ccaagacaac gatcggtagc cggactgaga
      841 ggtcgaacgg ccacattggg actgagaacg gcccagactc ctacgggagg cagcagtggg
      901 gaatattggg caatggaggc aactctgacc cagcaatgcc gcgtgaagga tggaaggccc
      961 tcgggttgta aacttcttta gacagggaag aaagaaatga cattacctgt agaataagcc
     1021 acggctaact acgtgccagc agccgcggta atacgtaggt ggcgagcgtt atccggaatt
     1081 actgggtgta aagggcgcgt aggcgggtac gcaagttaga tgtgaaatcc cggggcttaa
     1141 ccccggactg gcatctaaaa ctgtgtatct tgagtgtcag aggggaaagt ggaattccta
     1201 gtggagcggt aaaatgcgta gatattagga ggaacatcag tggcgaaggc gactttctgg
     1261 ctgacaactg acgctgaggc gcgaaagcgt ggggagcgaa caggattaga taccctggta
     1321 gtccacgccg taaacgatga ttactaggtg tagggggtat cgactccctc tgtgccgcag
     1381 ttaacgcatt aagtaatcca cctggggagt acggccgcaa ggttaaaact caaag
//
LOCUS       DASRMS010000140         1616 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_573997, whole genome shotgun sequence.
ACCESSION   DASRMS010000140 DASRMS010000000
VERSION     DASRMS010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_573997"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VFC74_06385"
     CDS             <1..189
                     /locus_tag="VFC74_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24997.1"
                     /translation="FESKVGSHLDYKTLPKSLNDYLPLDGKQTKTKSIEHQQAMEALR
                     RERAEAKARIEQLKKEQQ"
     gene            186..1328
                     /locus_tag="VFC74_06390"
     CDS             186..1328
                     /locus_tag="VFC74_06390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24998.1"
                     /translation="MSFTAVISANIKDFESNLQKAQSQMDAFDKKIGVSVASIGRAVE
                     GVGKKMSILSAGLVVAGGKAFTMAADFQDAIGATDQIFKDASDTVNDWAKNLSSDFGI
                     AKEEALKYSNLMGSMLINIGQLTEEQAAKQSAKLIELAGDLTAMYGGTTQDAVRALTG
                     ALKGNNTMLDNYGMAVNDALVKQKAFELGLSSGTGELSLQAKQAATLALIWEQTGAAQ
                     GQAGREADTASGAMRAVAVEVKNLSTEIGEVLLPIITPIVAKIRDFVAALRELDPKVL
                     EMGVKIAGVVAAIGPLLVMIGKVLQMLPLIKVAITALTGPVGLVVAAVAAAATLIIKY
                     WDEIKTFFINVANAVTQAIAKMAGNIAGLLEQPPGLSSLQQVFQRR"
     gene            complement(1231..>1616)
                     /locus_tag="VFC74_06395"
     CDS             complement(1231..>1616)
                     /locus_tag="VFC74_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK24999.1"
                     /translation="IKTFFINVANSVTQAIAKMASNIAGLLERVGMDKMASKLRSFQQ
                     TMEDAFIIPKPKVDDLENSVDELGNTVNGTTTNVNGLAGGLNGLGSQAEETANAFEKL
                     VAGMTAREAVQATPLYCLPSWLLPV"
BASE COUNT          498 a          320 c          390 g          408 t
ORIGIN      
        1 tttgaatcaa aggtcggttc acatttggat tacaagacgt taccgaaatc acttaacgac
       61 tatctaccac ttgacggcaa gcagacgaaa acaaaatcaa tagagcatca acaggcgatg
      121 gaggcactaa gacgtgagag ggcagaagcg aaagcaagga tagaacaact taaaaaagag
      181 caacaatgag ttttacggca gtcatatcgg caaatataaa agactttgaa agcaaccttc
      241 aaaaggcaca aagccagatg gatgctttcg ataagaagat aggtgtcagc gttgcaagta
      301 tcgggcgggc cgttgagggc gttggcaaaa agatgtctat actttccgct ggattggttg
      361 tcgctggtgg taaggcattc actatggctg ctgattttca ggatgcaatc ggagcgacgg
      421 atcaaatttt taaagatgcg tcggacacgg ttaatgactg ggcaaaaaac ctatcttctg
      481 actttggcat agcaaaagaa gaggcgttga aatattccaa cctgatgggg tcgatgttaa
      541 taaatatcgg tcaattaaca gaggagcaag cagcgaaaca atccgcaaaa cttattgagt
      601 tggcaggtga tcttactgcc atgtatggag gcactacgca ggatgcagtt agagccttga
      661 caggtgcact gaaaggtaat aacacgatgc tggataatta cggcatggct gttaatgatg
      721 cacttgtcaa acaaaaagca tttgaattag gattatcatc tggaactgga gaattaagtt
      781 tacaggcaaa acaggctgcg actttagcac ttatttggga gcagacaggg gcagcccaag
      841 ggcaagcagg aagggaagca gacacggcaa gtggggcaat gcgagcagtg gcagttgagg
      901 tcaaaaatct ttcaactgaa ataggagagg tattgttgcc tatcatcacg ccaatagtag
      961 cgaagatacg agattttgtt gctgcactac gtgagcttga tcctaaagtg ctggaaatgg
     1021 gagtcaaaat tgcgggagtt gttgctgcaa taggtcccct tttagtaatg ataggaaagg
     1081 tgttacaaat gttacctctt ataaaagtag ctataacagc gttaaccggt cctgttgggt
     1141 tagttgttgc agcagttgca gcggctgcaa ccttgatcat aaaatactgg gatgagataa
     1201 aaacattctt cattaacgtg gccaatgccg tcacacaggc aatagccaag atggcaggca
     1261 atatagcggg gttgcttgaa cagcctcccg ggctgtcatc cctgcaacaa gtttttcaaa
     1321 ggcgttagcc gtctcctctg cctgacttcc aagcccattt aaaccacccg ctaaaccatt
     1381 gacatttgta gtagttccat taacggtatt gccaagctca tcaacagaat tctcaagatc
     1441 atcaactttt ggttttggga taataaaagc atcttccatc gtttgttgaa agctcctcag
     1501 cttcgatgcc atcttatcca tcccaaccct ttcaagcaag cctgcaatat tgcttgccat
     1561 cttagctatt gcctgtgtaa ctgaattggc tacattaatg aaaaatgttt ttatct
//
LOCUS       DASRMS010000141         3198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_170211, whole genome shotgun sequence.
ACCESSION   DASRMS010000141 DASRMS010000000
VERSION     DASRMS010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3198
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170211"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            49..426
                     /locus_tag="VFC74_06400"
     CDS             49..426
                     /locus_tag="VFC74_06400"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HZK25000.1"
                     /translation="MNRNIFLVLALVVMAAFAWYVKGTVLVPAQTVAGPDLVSHRVDQ
                     YDEDCVDCHAKVGEWHKENFAAFSSDNCMDCHGGAPGTPHPTDGTYAECLTCHEPIVA
                     THDEMFPYENTTYEDCVGCHIPE"
     gene            604..1920
                     /locus_tag="VFC74_06405"
     CDS             604..1920
                     /locus_tag="VFC74_06405"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HZK25001.1"
                     /translation="MIYQDVKWIRTHGAKLAPSLRKHILAWYRPASFTPCFINNLYTN
                     IIQKWRKIPVIVQLDDSAPSTLSLQSISQTTGCKVQKDLSLLHAFSTKVDRKTLQALA
                     EDPKVVRIWYDEEIHAVLDTASPTVQAASGWEEGVTGKDVTIAIIDTGVYNHADLAGR
                     IVGFVDLVGSKQQPYDDNGHGTHVAGCAAANGKQSAGKYKAPAPEANIVGVKVLSKTG
                     SGSLSTVIQGVQWCIDNQARYKIRILNLSLGSETYQSYQDDPVGLAVAKAWDSGLIVC
                     VAAGNSGPEARTVNSPGNHPKVLTVGALDDGNTSDNDDDQIAGFSSRGPTADELNKPD
                     VVAPGVNIIALRSPGAYLDKQNKKARIEIDYLSLSGTSMATPVCAGAIAVLLEKDPTL
                     TPERVKQLLQATATRLPEYGDNDQGSGLINLQRALQELNPPLAKTE"
     gene            2312..3133
                     /gene="speD"
                     /locus_tag="VFC74_06410"
     CDS             2312..3133
                     /gene="speD"
                     /locus_tag="VFC74_06410"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HZK25002.1"
                     /translation="MEIKSFKKLKLYGFNNLTKSLSFNMYDICYAKTPEDRQGYIEYI
                     DEQYNAERLTSILTNVSEIIGANILNIAKQDYDPQGASVTILIAEGHVPEEHQYTEHT
                     QNETPGPLPDAIVAHLDKSHITVHTYPESHPDDGISTFRADIDVSTCGHISPLKALNY
                     LIHSFESDIITIDYRVRGFTRDVSGKKYFMDHKINSIQNYIPGNTREKYQAIDVNVYQ
                     ENIFHTKMILKEFDLDNYLFGICKKDLSASQRRRIKQRLKKEMLEIFYGRNISKV"
BASE COUNT          952 a          671 c          675 g          900 t
ORIGIN      
        1 gcagaatata tttcattgga atatttatct tataatctag gaggacatat gaacagaaat
       61 atttttctag tacttgcttt agttgttatg gctgccttcg cttggtatgt taaaggaact
      121 gtcctggttc ccgcacaaac ggtagccggt ccggatcttg ttagtcaccg cgtggaccaa
      181 tacgatgagg attgtgttga ctgtcacgct aaagtcgggg agtggcataa ggagaatttt
      241 gccgccttta gtagtgataa ctgcatggat tgtcatggtg gtgctcctgg caccccacac
      301 cccacagatg gtacatacgc tgaatgctta acctgtcacg aaccaattgt cgccacacat
      361 gatgagatgt tcccatacga aaatacaact tacgaggatt gtgtgggttg tcatatccct
      421 gaataagctt aacttctaaa gccgatacat ttattgtatc ggcttttttg ctggcagaga
      481 aaagataatc tcttcatgag tatgtagttc cgtactcaaa tagtcccgat acagagaaca
      541 aattggcgca taacctccat ccttttcata ttgtattgta tctacaaatg atggagggtt
      601 gagatgattt atcaagatgt aaagtggatt cgtactcatg gcgccaagct tgcgccttca
      661 ctgcgtaaac acatcctggc ttggtaccgg cctgccagtt ttacgccatg ttttatcaac
      721 aatttgtata caaatatcat ccagaagtgg cgtaaaatcc cggtgattgt ccaactagac
      781 gacagcgccc ccagtactct ctccctgcaa tcgatcagcc aaactaccgg ttgtaaagta
      841 caaaaggatc tttccttact gcatgccttt tcaacaaagg tcgatcgcaa aacactgcag
      901 gctttggcag aagatccaaa ggtagtgcgc atctggtatg atgaggaaat acacgccgtt
      961 ttagatacag cttcccccac tgtgcaggcc gctagtggct gggaagaagg tgttactggt
     1021 aaagatgtga ctattgccat cattgataca ggtgtttata atcacgcaga ccttgctgga
     1081 cgcattgtgg gctttgttga tttggttggc agcaagcagc aaccatatga tgataatggc
     1141 catggaactc atgtcgccgg ttgtgcagct gccaatggca agcaaagtgc aggcaagtat
     1201 aaggcaccgg caccagaagc aaatattgta ggcgtcaagg tattaagtaa gacaggttca
     1261 ggttctttaa gtacagtcat tcaaggtgta caatggtgta ttgataatca ggcacgttac
     1321 aaaattcgta tccttaacct ctccttaggc agtgaaactt accaatcata ccaagatgat
     1381 cctgttggtt tagcagtagc aaaagcctgg gattccggtt taattgtctg tgtggccgcc
     1441 gggaatagcg gaccggaggc acgtacagtt aattcacctg gtaaccaccc gaaagttttg
     1501 actgttggtg cacttgatga tggcaatacc agcgataacg acgatgatca gatagccggc
     1561 ttttccagcc gtggtccaac ggcagatgag ttaaataaac ctgatgtcgt agcgccagga
     1621 gttaacatta tcgccttacg ctcccctggt gcctatctgg ataaacagaa taagaaagcc
     1681 cgcatagaaa tagactattt atcgctgtca ggaacttcta tggctacacc cgtttgtgct
     1741 ggcgcgattg ccgttttgtt agaaaaagac cccacattaa caccagagcg agtaaaacaa
     1801 ctgctgcagg caacggcaac tcgtctgcct gaatatggcg acaacgatca aggcagcggc
     1861 ctaattaact tgcagcgagc tctgcaagaa ctaaatccac ccctagctaa aacggaatag
     1921 cacgctaaag cgtgctattc ttgttttagc tcttttattg tattggcaaa gcagctattt
     1981 tctgtttttg aggcatcgct atcaagtctc tttggagagt atatttttac tagctccaca
     2041 aagatagttt ttaagcatta gggcttcctt ttctctgaaa ttctgctata gtaatagtta
     2101 ttgaacaagc agtaaaaagg gacagcttgt gcaaataaat ggcttgctaa tcttgtagca
     2161 gcagtctatg taggtttata atgaagcacg agtgatatac tacaggagga tatgccttaa
     2221 aactcagtag cactttttag ttaaatctgc tccctcacag cgaagtaaat aatcactagc
     2281 agtgaataaa aaatgttttg ggatgtgaat tatggaaatt aaatctttta aaaaattaaa
     2341 actttatggt tttaataatc taacaaaatc attaagtttc aatatgtacg acatttgtta
     2401 tgcgaaaaca ccagaggacc gtcaggggta tattgagtat attgatgaac agtacaatgc
     2461 tgagcgactt acctctattc ttaccaatgt ctccgagata attggtgcaa acatcctcaa
     2521 tattgccaag caggattatg acccccaagg ggccagcgtg acgatactca ttgctgaagg
     2581 tcatgtccca gaagagcacc agtacactga acatacccaa aatgaaacac ctggtccact
     2641 accggatgct attgtggcgc atttagataa aagccatatc actgttcata cctatccgga
     2701 aagccacccc gatgacggca ttagtacttt tcgtgctgac attgatgttt ccacttgcgg
     2761 tcacatttct ccactaaaag ctctaaacta tttaattcac agttttgagt ctgacattat
     2821 cactattgac tatcgggtac gtggttttac gcgtgatgtc agcggcaaaa aatattttat
     2881 ggatcataaa attaactcta tccaaaatta tattcctggt aataccagag aaaaatatca
     2941 agccatcgat gttaatgtct atcaggaaaa tatctttcac actaaaatga ttctgaaaga
     3001 gttcgaccta gataattatc tctttggtat ttgtaaaaaa gatctttctg ccagtcaacg
     3061 ccgaagaata aaacagcggc tcaaaaaaga aatgttagaa atcttctacg gccgcaacat
     3121 ctctaaagtc taaaaagcga aacaaacgtt tcgctttttt gcaataaaga taattaagca
     3181 gcctctttct acaatggc
//
LOCUS       DASRMS010000142         1118 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_305965, whole genome shotgun sequence.
ACCESSION   DASRMS010000142 DASRMS010000000
VERSION     DASRMS010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1118)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1118)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1118
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_305965"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..679)
                     /locus_tag="VFC74_06415"
     CDS             complement(50..679)
                     /locus_tag="VFC74_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25003.1"
                     /translation="MKPKKLVYGVGNNDADYAVVKFETIDCADGKRKQKQVWTCPYYK
                     VWRDMIKRCYSAKFQESRPTYIGCTVSDKWLTFSNFTGWMKNQDFEGKQLDKDLLFEG
                     NKVYSPETCVFVTGAVNSFTTDSGAARGDCLIGADWYKQTGKFRSKCSNPFTKKPEHL
                     GYFTSEQEAHEAWAKRKLELAHELAAIQTDQRVAKALIDRYAQPMEKTE"
     gene            complement(830..1036)
                     /locus_tag="VFC74_06420"
     CDS             complement(830..1036)
                     /locus_tag="VFC74_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25004.1"
                     /translation="MKTLTAVPISELIKKAGSKRKVAQIAGIQNLGSLSHESVLIDGR
                     MYRPSISTADWAEVYKQLVEGKNE"
BASE COUNT          276 a          290 c          206 g          346 t
ORIGIN      
        1 gtaacacaca atgcgcacgg gcgtgtagat aacagtatac cactaaacct tattctgttt
       61 tttccatcgg ctgcgcataa cgatcaatca atgctttagc caccctttga tctgtttgaa
      121 ttgccgccag ttcgtgcgct agctccagct tgcgttttgc ccatgcttcg tgggcttctt
      181 gttcagatgt gaagtagcca aggtgttccg gttttttagt aaatgggttg gagcatttag
      241 atcgaaactt gcctgtttgc ttataccaat ccgccccgat caaacaatca cctcttgctg
      301 ccccactgtc agtggtgaag ctgttcaccg ctcctgttac aaacacacat gtttcagggc
      361 tataaacctt attcccttca aacagcaaat ccttatcaag ttgctttccc tcgaagtctt
      421 ggtttttcat ccagcctgta aaattgctga acgtgagcca tttatctgat acggtgcagc
      481 caatgtaagt cggacggctc tcttgaaatt tggcagaata gcacctctta atcatgtctc
      541 tccaaacttt gtaatagggg catgtccaaa cctgcttttg ctttcgctta ccatccgcac
      601 aatcaatcgt ctcaaacttc acaacagcat aatctgcatc attattaccc acgccgtaga
      661 ctagcttctt tggtttcata tttatgatcg tccataaaaa aaccgttatg ctcacccttt
      721 tcgccagaaa agccagtctt agtaactggt gatgagcata acggttttta ctaagtatct
      781 tttctggcag atctaattat acgcactgcc gataacaatg caatggtttt tattcgtttt
      841 taccctccac cagctgttta taaacctcgg cccaatccgc agttgaaatg ctcgggcgat
      901 acatacgacc atcaatcaac acagattcgt gagacaagct acccaagttt tgaataccgg
      961 caatctgcgc caccttccgc ttgctgcctg ctttttttat tagctcgctg attggtactg
     1021 ctgtgagtgt tttcatttgt cacccctgcg ccatgcgcga gcaatagcct taaaattata
     1081 gtactcgtct aaaccaagat cgactttcag atcctcga
//
LOCUS       DASRMS010000143         4132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_35159, whole genome shotgun sequence.
ACCESSION   DASRMS010000143 DASRMS010000000
VERSION     DASRMS010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35159"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..430)
                     /locus_tag="VFC74_06425"
     CDS             complement(89..430)
                     /locus_tag="VFC74_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25005.1"
                     /translation="MKNTHNFRMPDQSINALIDSAPNTHEPLSSAADTSLHYYCLAFM
                     YAEKDAKGSIYVGLLEEKITTKVITAAKNKMLKNYGADPDTAVLTSVSYLGFMKPSEF
                     APDNWPEGLLE"
     gene            638..826
                     /gene="csrA"
                     /locus_tag="VFC74_06430"
     CDS             638..826
                     /gene="csrA"
                     /locus_tag="VFC74_06430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004155916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HZK25006.1"
                     /translation="MLILTRSQGETICIGDDIRITVLGTIGGQVRLGTDAPQNVEVHR
                     EEIYQRIQLEKRAREQPK"
     gene            1756..3504
                     /locus_tag="VFC74_06435"
     CDS             1756..3504
                     /locus_tag="VFC74_06435"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HZK25007.1"
                     /translation="MFIKKALVWVLSVALSSAVSFSVQAQIIPELEQKAERGDAKAQF
                     DLGGRYDRGEGVTQDYKKAVYWWHKAAEQEYAKAQFNLGVMYANGDGVAQDYKQAVHW
                     YRKAAEQGDADAQSKLGDMYVYGNGVAKDSKQAEQWWRKAAKQGHVGTQSKLGVFYAV
                     GIVVAQDDKQAMHWYRKAAEQGDVSSHINLGDRYYNGKGVAQDYKQAMHWYHKAAEQG
                     DAGAQFNLGNRYYNGEGVAQDYKQAVHWYRKAAEQGYVNGQHYLARSYYKGKGVSQDY
                     MQAVQWYRKVAEQGYARGQLRLGFMYENGYGVAQDYKHAVHWYRKAAEQGDASAQVSL
                     GNRYYYGEGVAQDYKQAVHWYRKAAEQGDADAQSKLGDMYKNSYGVAQDCKQAVHWYR
                     KAAEQGNATAQNNLGISYGNGDGVAKDYKQAVQWWRKAAEQGDASAQFNLGLSYYNGY
                     GVAQDHKQAVQWYRKAAEQGDARAQHLYVELSYAGGEIAPAEIEQMVRWLRASAQQGE
                     AEAQYYLGRLFNSGDGIPQNTTLAYAWLSVAVANGYDKARSLRDEIAQNLSPVALDKG
                     QTLAAEYFEKYRPSSP"
     gene            3601..4032
                     /locus_tag="VFC74_06440"
     CDS             3601..4032
                     /locus_tag="VFC74_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25008.1"
                     /translation="MEVFWIIFVQLITALIIIGVVYKVRPGELEKVKKENNRLKEKLK
                     SNEDVSIELKKSELIFTDKISKIERQLNTLKGKESTYQKEISELKVKYAQSQKFLKNC
                     HKALNRNGYPIPKNPQVTLESKNFDWCNACDRNIRNCMCSN"
BASE COUNT         1167 a          780 c         1155 g         1030 t
ORIGIN      
        1 gtgttgaagt gatcaaccag catggctgca atgcctatta ttttcattgt ggtcaggctg
       61 gtgttggaaa tacgcaatgc gtcaagggtc actccaaaag cccctcaggc cagttatcgg
      121 gggcgaactc gctaggtttc atgaagccaa ggtatgaaac tgaggtcaaa acagctgtat
      181 ctggatccgc tccgtagttt tttagcatct tgttttttgc ggcggtgatc actttggtgg
      241 tgattttttc ttccaacaga cctacatata ttgacccttt tgcgtctttt tcggcataca
      301 taaaagcaag gcagtaatag tgcagagacg tatcagcggc gcttgacagc ggctcatggg
      361 tatttggagc gctgtcgatt aaagcattga tggactggtc aggcatacga aagttatgtg
      421 tgtttttcat tgagttacac cttgaattaa cgctcacgct ttcaccgggg ttagccccgg
      481 atatgttgga tgtcctagta ttatctgctt ggtggtctga gttgtgtgct gttaatccga
      541 tactggcttt ttcggtttgt ttcagtcagt agattttgat aggtttgcct aagcacttgt
      601 ttggtgccta tgcgatctaa taaaaaggag agtacggatg cttattttga cgcgcagcca
      661 aggcgagacg atttgcattg gtgacgatat acggattacg gtgctaggca ccattggtgg
      721 tcaggtacgt ttgggaactg atgcacctca gaatgttgag gtgcaccgag aggaaatcta
      781 ccaacgaatc caattggaaa agcgggcaag agaacagcct aaataatgcc tacaacttag
      841 gtgggttttt aggacactga tagcttccca tcttgatgaa gggaagctat gcctcccgat
      901 ttgactcaaa gattgtttta taaagtaaaa gcaaatcact gacttacttt atcagtcaaa
      961 aagagttttt cgcgccgcga cagccaagtt ggaccacgtg gcttcgttat ctcaactcaa
     1021 ctcaacttca ggctggccta gcggagttgt cattcgctag gagatgacat ctaataacga
     1081 tcataaatcg ctaaggatgg tgaccttcct acagaggtct ttcacctcat tagttcatgc
     1141 ccatgctggg cgtacacaag gccaatcaca aaggccaaca agctggtcgg actggctacg
     1201 ctcgcctgtt ttagcggcgt tatgtgcaac caaagcagga gcacaccatg aaccttagag
     1261 cagcattgct cgctttatgc cttattagca ttagctcact ttcaagtgcg ggtcccactt
     1321 gtcgcactga ttcttttggt actacccggt gtgataatgg agctacttat cgcacagact
     1381 catttggcac tactcgtgac aaccaaggaa attcatggcg tacggattcc tttggtacta
     1441 cgagaggaag tgatggctca acttatagga ctgattcatt cggagctaca cgagacaacc
     1501 ggggcaattc ttggagaaca gactctttcg gtacgacgag aggtagcaac ggttctactt
     1561 gccgtaagga ctcacacggc accactcgct gtaattaaac acataaccag agcattaaat
     1621 tcgttcctag cctagtcggg ccgtttatgc aggcgctagg cgtagggaca tgttgtcaaa
     1681 gtgagttctt tagaagaagt gatatgagtc ccgacttcgg gttagttagt ttaaaaacaa
     1741 ggaaaggaag tagtcgtgtt tataaagaaa gctttggtat gggtgttaag tgtagcgctt
     1801 agcagtgcgg tgagttttag tgttcaggcg cagattatac cggagctcga gcagaaggcc
     1861 gagcgtgggg atgcaaaagc gcagtttgac ttagggggta gatacgatag aggtgaaggt
     1921 gtcacgcaag actacaagaa ggcagtgtat tggtggcata aagcagctga gcaggagtat
     1981 gcgaaagcgc agtttaactt aggggtcatg tatgcaaatg gcgacggggt tgcgcaagac
     2041 tacaagcagg ctgtgcattg gtatcgcaag gctgcggagc agggggatgc ggatgcgcag
     2101 agtaaattag gggatatgta tgtttatggc aacggcgtcg cgaaagacag caagcaggct
     2161 gagcagtggt ggcgtaaggc agcgaagcag gggcatgtgg ggacacagtc aaaattagga
     2221 gttttctatg cagttggcat cgtcgtcgcg caagacgaca agcaggctat gcattggtat
     2281 cgcaaggcag cagagcaggg ggatgtgagt tcacatatta acttaggaga tagatactat
     2341 aacggtaagg gtgtcgcgca agactacaag caagctatgc attggtatca caaggcagcg
     2401 gagcaggggg atgccggagc acagtttaac ttaggaaata gatactataa cggtgagggt
     2461 gtcgcgcaag attacaaaca ggctgtgcat tggtatcgca aggctgcaga gcaggggtat
     2521 gtgaacggac agcactatct agcacgtagc tactataagg gtaagggtgt ctcgcaagac
     2581 tacatgcagg ctgtgcagtg gtatcgtaag gtagcggagc aggggtatgc gaggggacag
     2641 cttcgcttag gttttatgta tgagaatggc tacggcgttg cgcaagacta caaacatgct
     2701 gtgcattggt atcgtaaggc agcagagcag ggggatgcca gcgcgcaggt ttccttaggg
     2761 aatagatact attacggtga gggcgtcgcg caagactaca agcaggctgt gcattggtat
     2821 cgcaaggcgg cggagcaggg ggatgcggat gcgcagagta aattagggga tatgtataaa
     2881 aatagctacg gcgtcgcgca agactgcaag caggctgtgc attggtatcg caaggcagcg
     2941 gagcagggga atgcgacagc acagaataac ttagggatta gttatggcaa tggcgacggc
     3001 gtcgcgaaag actacaagca ggcggtgcag tggtggcgta aggcggcgga gcagggcgat
     3061 gccagcgcgc agtttaactt agggcttagt tattataatg gttacggcgt tgcgcaagac
     3121 cacaagcagg cggtgcaatg gtatcgcaag gcagcggagc agggggatgc gagagctcag
     3181 cacttatacg tggagctgag ctatgcgggt ggtgagattg cacctgcaga gatagaacag
     3241 atggttcgct ggttgcgtgc atcagcccag cagggagagg cagaggcgca gtattactta
     3301 ggaaggttgt ttaatagcgg tgatggcata ccacaaaaca caacattggc ttatgcgtgg
     3361 ttatcagtgg cagttgccaa cgggtacgat aaagcacgat cgcttcgtga tgaaatagcg
     3421 caaaatcttt ctccggtagc gctagacaag gggcagacct tggctgcgga gtattttgaa
     3481 aagtatcggc caagcagtcc ttagcctaaa gcatcttatc cgcactctac atctaaagat
     3541 ctcgtgcacc aggaaatgta taaagtcaaa tagactaacg aaaatagcag gcgtgtggtt
     3601 atggaagtgt tttggattat ttttgtacag ttgataactg ctttaataat aattggtgtt
     3661 gtttataaag ttaggcctgg agagttagag aaggtaaaga aagaaaacaa tagattgaaa
     3721 gaaaaattaa aaagtaacga agatgtttct attgagctta aaaagagcga gcttatattt
     3781 actgataaaa tatcaaagat agagaggcag ctaaatactc tcaaaggaaa agagagtact
     3841 tatcaaaaag aaataagtga gctaaaagtt aaatatgcac agagtcagaa gtttttaaaa
     3901 aactgccata aagcactgaa tagaaatggg tatccaatcc caaaaaaccc tcaagtcaca
     3961 cttgagagta aaaactttga ctggtgtaat gcctgtgata gaaatatacg taactgtatg
     4021 tgttcaaatt aaaatgctaa ctaggtaact gaaaatttat tatcaaataa cccatggtct
     4081 ttttgctgct gtcgtgattg agcatttatc agtatttatc ccgtgttact ga
//
LOCUS       DASRMS010000144         1054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_507755, whole genome shotgun sequence.
ACCESSION   DASRMS010000144 DASRMS010000000
VERSION     DASRMS010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1054
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_507755"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /locus_tag="VFC74_06445"
     CDS             complement(<1..838)
                     /locus_tag="VFC74_06445"
                     /inference="COORDINATES: protein motif:HMM:NF019315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7TM diverse intracellular signaling
                     domain-containing protein"
                     /protein_id="HZK25009.1"
                     /translation="MDNADDLANVISQLNLNATQPLLVGMLLGCNISLILYNLLRFIY
                     TRYYSSLCFALALGFVLLFLFFSIEPLTAHTPFLLNLDSDFIYISVVLSMLFLHILCL
                     NLLPQTAVFSGLSKQLIRHMGALCLSGIALLTLTAAHAHTLTFYVLITSAVMLLGSTL
                     LLWHNDYTPARTLSIGFSVFYLPLLAAWPAFNNESLFSADVAVLLTLTSLAISAFIIS
                     IGLSEAHRYQIKKNVRVSQRHAAEEAESRAKSELLAKISHEIRTPLSGVLGMTTLLLD
                     TPL"
BASE COUNT          331 a          213 c          262 g          248 t
ORIGIN      
        1 acaatggagt gtccagtaat aaagtagtca tgcccaacac accgcttaaa ggcgtgcgta
       61 tttcatggct gattttagct aataattctg atttggcacg gctctcagct tcttcagcag
      121 catggcgttg tgaaacacgg acgttctttt taatttgata acggtgggct tcactgagcc
      181 cgatgctaat aataaacgca ctgatggcga ggctggttag agtcaataaa actgcgacat
      241 cggctgaaaa taaagactcg ttattaaagg ccggccaagc cgctagcaaa ggtaaataga
      301 aaacactgaa cccaatggat aacgtacgtg cgggtgtgta gtcgttgtgc cagagcagca
      361 gagtgctgcc taataacata accgctgagg tgataagcac ataaaaagtt agagtgtgag
      421 cgtgggctgc tgttagggtg agtaaggcaa ttccgcttaa acatagggcg cccatatgtc
      481 gaatcaattg cttagatagg ccgctgaaca ctgctgtttg tggcagtaga ttcaaacaga
      541 ggatatgtag aaataacatg cttagtacaa cagaaatata gataaaatca ctgtcgaggt
      601 taagcagaaa aggtgtgtgt gcggttagtg gctctattga aaaaaacaaa aatagcagca
      661 caaaacccag tgccaaggca aagcataggc tgctgtagta gcgtgtataa ataaagcgca
      721 gaagattata taaaataagg ctgatgttac aacccagcag catgccgacc aagagtggct
      781 gcgtcgcatt taagttcaac tggctgataa cgttcgccaa gtcatcagca ttatccaatg
      841 aaaacactaa ggcgtagtaa ttgggggtgc tgatcatcaa aaacagagag cctaagatca
      901 ggctgatgat taaagcgcta gctaggcgaa tcggtttcat gtcagtaatc agcgcctctt
      961 gagtaccaac cacgctcccc gcatacaatt agcagcgggg agcaccggta gtatttactg
     1021 ctgttcgcgc tcgatagcac gataaccgat atct
//
LOCUS       DASRMS010000145         1172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_372540, whole genome shotgun sequence.
ACCESSION   DASRMS010000145 DASRMS010000000
VERSION     DASRMS010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1172
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372540"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..651)
                     /locus_tag="VFC74_06450"
     CDS             complement(<1..651)
                     /locus_tag="VFC74_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="MutS2; MutS-II; involved in blocking homologous and
                     homeologous recombination; has ATPase activity stimulated
                     by recombination intermediates; inhibits DNA strand
                     exchange; Derived by automated computational analysis
                     using gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZK25010.1"
                     /translation="MITGSLRKLEFPSIIRLLSQQADTATGRKLCEQLLPAENKKEAE
                     IRLRETDDAVKRLLEYGGLPFGGIQDIHSQISRASNGAVLSGRDLLKIAALLRSVVRM
                     RQAIPEVWTAAKKATDKENFPYNAIYHRIESLTLCRDLQQEIENSVAGEDEIFDRASP
                     ELMSIRRRIREAQASIRSELDKILRKHSSVLQEALITVRGDRYVVPVKSEQRSAVPG"
     gene            complement(648..>1172)
                     /locus_tag="VFC74_06455"
     CDS             complement(648..>1172)
                     /locus_tag="VFC74_06455"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HZK25011.1"
                     /translation="LTLVRLLAIVPLVILLSYWPKYKTTSFIFFLSIWLTDMLDGYIA
                     RTFNQITDFGKLFDPVVDKLFQIAVAVTLAAIGRLPVWVPTFIVCREALMILGGYLLL
                     KNYEVVVYSDVFGKLATVFYVIGFTLLFFVPDEPGWLRHVIFIPGVALSVIATLNYAR
                     KNIDNIKNRKKAAV"
BASE COUNT          349 a          276 c          240 g          307 t
ORIGIN      
        1 gccgggaaca gcagaacgct gctcgctttt aaccggcaca acgtagcggt cacctcgaac
       61 agtaatcaaa gcttcctgta aaacagaact gtgcttgcgc agaatcttgt ccagttctga
      121 tctgatactt gcctgcgctt ctctgatgcg cctccggatc gacatcagtt ccggactggc
      181 gcggtcaaaa atttcatctt caccggcaac tgagttttct atttcctgct gaagatcccg
      241 gcagagggtc aatgattcaa tccggtgata aatagcattg tagggaaaat tttctttatc
      301 agtagctttt ttagcagctg tccagacttc aggtatggct tggcgcatac gtacgaccga
      361 ccgtaaaaga gctgcaattt tcagtaaatc acgtccgctg agcacggctc cgttactggc
      421 acgactaatc tgggaatgaa tatcttgaat accaccaaaa ggcaaaccac catattccaa
      481 caagcgtttt accgcatcgt cggtttcccg caaacggatt tccgcttctt ttttgttttc
      541 agccggtagc agttgttcgc ataatttgcg tccggtagca gtatcagctt gctgggataa
      601 taaacggata atgctgggaa actctaattt cctcaaactt ccagttatca tacggcagct
      661 tttttcctat tctttatgtt atcgatattt tttctggcgt aattcaaagt agcaattact
      721 gataaagcaa caccgggaat aaagattaca tgacgcaacc aaccaggttc atccggaaca
      781 aaaaagagta aggtaaagcc tattacgtag aaaaccgtgg ccagtttccc aaacacatct
      841 gaatagacaa ctacttcgta gttttttaac aaaagataac cgcccaaaat cattaatgct
      901 tcacgacaca caataaatgt aggcacccaa accggcagac gcccgatcgc tgccaaagta
      961 acagccacag ctatttggaa aagcttatca accaccgggt cgaaaagttt accaaagtca
     1021 gttatttgat tgaaagtacg ggcaatatat ccatccagca tgtcagtaag ccatatggac
     1081 aaaaagaaga taaatgatgt tgttttatat ttaggccagt aactcagcaa tatcaccagg
     1141 ggtactatcg caagcaagcg cacgagtgtg ag
//
LOCUS       DASRMS010000146        10232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_36053, whole genome shotgun sequence.
ACCESSION   DASRMS010000146 DASRMS010000000
VERSION     DASRMS010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36053"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..266)
                     /locus_tag="VFC74_06460"
     CDS             complement(51..266)
                     /locus_tag="VFC74_06460"
                     /inference="COORDINATES: protein motif:HMM:NF020945.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl-phosphate phosphatase Spo0E family
                     protein"
                     /protein_id="HZK25012.1"
                     /translation="MGALHLEQVCKEDKDGWKEAVAGELEQERKKLYVLATNLGVSHP
                     TVLKQSRYLDKLVFSAMKKRNHNLFSF"
     gene            complement(500..1927)
                     /gene="gatB"
                     /locus_tag="VFC74_06465"
     CDS             complement(500..1927)
                     /gene="gatB"
                     /locus_tag="VFC74_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HZK25013.1"
                     /translation="MSNYQTVIGLEVHVELATKTKIFCGCPTEFGKEPNTHVCPVCLG
                     LPGTLPVLNKKVVDFAIKAALALNCEIPAFAKLDRKNYFYPDLPKAYQISQYDLPLAV
                     NGHLDIEVNGQTRRIGITRVHIEEDAGKLVHAPFGDYSLADYNRAGVPLIEIVSEPDL
                     RSPVEAKAYLEKLKSIIQYTGVSDVKMEEGSLRCDANVSIMPVDSQQFGTRAEIKNMN
                     SFRAVQNGLEYEVARQAEVLAAGELVIQETRRWDENKGITYAMRSKEEAHDYRYFPDP
                     DLVPIIVEPAWVEEIRQSLPEMPEARQKRYVTTWGLPAYDAAVITAAKSMADFFEATM
                     EAYHDAKAVSNWLMGEVTKHLNAEGLEITQTKLKPAHLIELLELQNKGTVSGTIAKTV
                     FEEMFKSGKMPAEIVKEKGLVQITDEAAIAAIVDEVIANHPKPVADYKSGNKKAIGFL
                     VGQVMKLSKGKANPPLVNKLLREKL"
     gene            complement(1920..3395)
                     /gene="gatA"
                     /locus_tag="VFC74_06470"
     CDS             complement(1920..3395)
                     /gene="gatA"
                     /locus_tag="VFC74_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HZK25014.1"
                     /translation="MLLEQLTVADAVEKLQSREISAQELTEATLKRIAAVDETVKAYL
                     TITSEQALAAAAQIDAKRKNGEQVGPLAGVPVALKDNMCTKGVKTTCASKMLHNFLPP
                     YDATVVELLQKAGCIMLGKTNLDEFAMGSSTENSTYFTTRNPWDLTRVPGGSSGGSAA
                     AVAADEALFAIGSDTGGSIRQPASFCGVVGMKPTYGRVSRYGLIAFASSLDQIGPLTK
                     DVRDCAMVMNAIAQYDPRDTTSAKQATPDYTSYLDKNVKGLKIGVPKEYFAQGITPGV
                     AAAVQKSIDLLVSLGAEAEEMSLPHTEYGIPIYYIIAPAEASSNLARFDGVEYGFRVE
                     TDNLLDMYKKTRSQGFGTEVKRRIMLGTYALSSGYYDAYYHKALQVRTLIKEDFDRAF
                     AKYDVILTPTTPATAFVAGTVEDPLTMYLNDICTIPANMAGLPAISLPCGFVDDLPVG
                     MQLIAKPFAEGTILRAAHAYEQHSGLPKQKPALEMGVSKDE"
     gene            complement(3404..3691)
                     /gene="gatC"
                     /locus_tag="VFC74_06475"
     CDS             complement(3404..3691)
                     /gene="gatC"
                     /locus_tag="VFC74_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HZK25015.1"
                     /translation="MKITDQEVAHVAALARLDINVSEMQQFTGELNKILEYIGKLNEL
                     DTKDIQPTAHVLPLRNVLREDKEQPCLPHGQALANAPETEDGMFRVPLVIE"
     gene            4025..5767
                     /locus_tag="VFC74_06480"
     CDS             4025..5767
                     /locus_tag="VFC74_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZK25016.1"
                     /translation="MNNLNLKQSRRKNGRVYLSIEKSYRDKVTGKPKARTVKSLGYLD
                     ELEKEYQDPIAHFKEIARKMTEEENKRKKLTLTLNMDEQLAQGTDNRKNLGYAAILKI
                     YHELGLHRFLNNKARNEDFKFNTNSIMMLLVVSRLLSPGSKKKTFEEKDRYFERFNFS
                     LPDVYRALSHYAKIAKDLQQFLNEQITEKYGRDTKTVYYDVTNFYFEIDKPDEVRKFG
                     PSKEKRPNPIIQMGLAMDANGIPLHYELFPGNKLDKETFRSVIGEVRKNYDTGRIVVV
                     ADMGIITGDNIYYLTGGKNRNGYVFSFSVRGGANAFKNYVLDNEGYVGTDGKPAGEDA
                     EFKIKSRRIAREINVTMKSGKKAKKTVYEKQVVFWSKKYAVKAKAEREELVKKALDLV
                     ASPQKYNKATSYGAAKYVKHLEFDKKTGEIIEGKKKPYFDADKLAEQEMYDGYYAIVT
                     SEMQMTDGEIIDTYRGLWEIEETFKVTKGTLEARPVYVSREDRIEAHYLTCFIALVII
                     RLIQKKMDRRYSPKMIIECLNKIACSNEQDNLYLFDYRSEISDDIGNVLGIDFTRKRL
                     RLADIKNILANCKK"
     gene            6180..7397
                     /locus_tag="VFC74_06485"
     CDS             6180..7397
                     /locus_tag="VFC74_06485"
                     /inference="COORDINATES: protein motif:HMM:NF019244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HZK25017.1"
                     /translation="MKHKHRWLTVTGYMLLLVVIGLVWPAEGQFVSAYRQILKASGVL
                     VSDYFVIGSISAALVNAGLVGLSGLALIYFTHTTLSGPTIAAVFTMAGFALFGKTPLN
                     IWPIFLGVALSAWLKKEQFRTYIVVALFGSALGPVVSQVAFGLGLGYLTGAVIGLLIG
                     IVLPALAPHLLQNHQGFSLYNIGFTCGIIGMFVVALLKLRGLDPTATMVWGSEQSTAL
                     AAVFLLYFFSMLILGWKGRRGARELLKLPGTLVTDFVSEQGFRASLFNMGLVGLMALA
                     YIILVGGAVNGPTLGGVFTIAGFGAFGKHPRNCWPLMAGVFIGSHLSVWTAAEPGPLL
                     AALFGTTLAPIAGSFGPLFGMAAGVVHLAVVMHVGIFHAGLNLYNNGFAGGLVGTLFV
                     SLSRWFNLQSKHD"
     gene            7412..7579
                     /locus_tag="VFC74_06490"
     CDS             7412..7579
                     /locus_tag="VFC74_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25018.1"
                     /translation="MDKDRKNITGIVVELMKNALQTNATSVNISFTEADEEIRTTNIP
                     TFVVLILVNIS"
     gene            complement(7591..8292)
                     /gene="sigK"
                     /locus_tag="VFC74_06495"
     CDS             complement(7591..8292)
                     /gene="sigK"
                     /locus_tag="VFC74_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigK"
                     /protein_id="HZK25019.1"
                     /translation="MIPIITIVATVIREITLLVSYLNNNSFPRPLSPQQEQVYLQRLA
                     EGDPEARSVLIERNLRLVAHVIKKYENTGEDQEDLISIGTVGLIKAIDSYKTNKGTRL
                     ATYAARCIENEILMHIRVITKRKAEVLLHDPIGTDKEGNEITLLDILGTDAEEVLEQV
                     DKKLLEAKLYEVIRKLKKRERTVLALRFGLPMGRRQTQKEIAKTLGISRSYVSRIEKK
                     VIAKIAEEFQHENIR"
     gene            complement(9099..9362)
                     /locus_tag="VFC74_06500"
     CDS             complement(9099..9362)
                     /locus_tag="VFC74_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25020.1"
                     /translation="MKLYHDDVRNLLEEIPCQDYDFIVLDLGIVFEPETDSEKRKEMV
                     QYKVHQFKEYGGIVLCCVNRLDVNQNHTLNTLNESINYARCHF"
     gene            complement(9593..9907)
                     /locus_tag="VFC74_06505"
     CDS             complement(9593..9907)
                     /locus_tag="VFC74_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HZK25021.1"
                     /translation="MAAGLKPPTGAGDQYWLNQWAKIISECRISGQTVVREAACETLP
                     APGENSSIVPVNISIEAAGTGLTETSSPITLRLGSVTLELRNNASVTLIENTLKALQN
                     AR"
BASE COUNT         2842 a         2304 c         2158 g         2928 t
ORIGIN      
        1 ccagtaaata atataattat gattatgctt cacggccgat gcctgtaaac ttaaaaagaa
       61 aatagattat gattcctttt tttcatcgca gaaaaaacta gtttatcaag atatctactt
      121 tgtttaagca ctgttggatg agaaactccc aaattggttg ctaaaacata tagttttttt
      181 ctttcttgct ccagttcacc agctaccgct tctttccacc cgtctttgtc ttccttacaa
      241 acttgctcta aatgtaacgc acccataccc ccaagcacct cccaaattcg cttgacctaa
      301 tctctaaaac acaatcctta tctaaaatat agcattgaac caagatatgc aaggggaata
      361 atgtagaatt ttgtagtcga tgaaaacttt tttattaatt taggaataaa actttttctt
      421 atgcatataa tgttggctgg tcttaatcag cctaaaataa aaaaagacgg atatcatcac
      481 aacatccgtc ttttttgcac tataattttt cccttaacag tttattgacc agtggcgggt
      541 ttgctttgcc tttagataac ttcatcactt gaccaacgag gaagccaatt gcttttttgt
      601 taccgctttt gtaatccgca accggtttag gatgattggc gatcacctcg tcaacaatgg
      661 cagcaatcgc agcttcatct gtgatttgga ctaacccttt ttctttaact atctcagctg
      721 gcattttgcc gcttttaaac atttcctcaa acaccgtttt ggctatggta ccgctgacag
      781 tacctttatt ttgcagttct agcaattcga ttaagtgagc tggctttagt ttagtttgcg
      841 taatttctag gccctcggca ttaaggtgct tggtaacttc ccccattagc cagttagaga
      901 cagctttggc atcatgataa gcttccatcg tggcttcaaa gaaatcagcc atacttttag
      961 cagcagtaat cactgcagcg tcataagctg gtaaacccca ggttgtaaca tacctttttt
     1021 gccgtgcttc cggcatttct ggcaggcttt ggcgtatttc ttccacccac gcgggttcaa
     1081 caatgatcgg cacgaggtcg gggtcaggga aatatctgta atcatgcgct tcttccttag
     1141 aacgcatggc ataagtaata cctttattct catcccaacg ccgagtttct tggattacca
     1201 gttcacctgc agccagcacc tcagcttgcc ttgctacctc atactccaag ccattttgta
     1261 cggcgcggaa agagttcata tttttaattt ctgcgcgtgt gccaaactgc tgactgtcga
     1321 caggcatgat agaaacgtta gcgtcacaac gcaaactgcc ttcttccatt tttacgtcag
     1381 aaacaccagt gtactgaata atgcttttta gcttttccag atatgctttc gcctcaactg
     1441 gcgaacgtaa gtccggttct gagactattt caatcagcgg tactcctgca cgattgtaat
     1501 ctgccagcga ataatctccg aaaggggcat gcactaattt accggcatct tcttctatat
     1561 gtacacgtgt aatgccaatg cgccttgtct gaccattaac ctcaatatcc aaatgaccat
     1621 ttacagctaa aggcagatcg tactgggaaa tttgataagc cttgggcaga tcagggtaaa
     1681 aatagttttt gcggtctaac ttggcaaagg cgggtatttc acagttgagg gctaacgccg
     1741 cttttatggc aaaatcgacc acttttttat tgaggacagg caaagtgcca ggcaagccaa
     1801 gacagacggg acagacatgt gtattgggtt ccttgccgaa ttcagtagga cagccgcaaa
     1861 aaattttagt ttttgttgct agctctacat gtacctctaa cccaattact gtttgatagt
     1921 tactcatctt tgctaacccc catttcaagc gcgggttttt gtttaggcaa accactgtgt
     1981 tgttcgtaag catgggcagc acgcaggatg gtcccttcag caaaaggctt agcaattaac
     2041 tgcataccga caggcaaatc atcgacgaac ccacagggga gcgaaatagc cggtaacccg
     2101 gccatattag ccgggatagt gcaaatatcg ttgagataca tcgttaaggg atcttccacc
     2161 gttccggcaa caaaagcagt agctggcgtc gtcggcgtta ggattacatc atatttagca
     2221 aaggcacggt cgaaatcttc tttgatcaaa gttcgtactt ggagtgcctt gtggtaataa
     2281 gcatcataat aaccggagct tagggcgtac gtacccaaca tgatacgccg ttttacttcc
     2341 gtgccgaagc cctggctgcg tgttttttta tacatatcaa gcagattgtc tgtttctaca
     2401 cgaaaaccgt attccacgcc gtcaaaacga gccaggttac tagaagcttc ggctggtgca
     2461 ataatatagt agataggaat gccatactcc gtatgcggca gtgacatttc ttccgcttct
     2521 gctcctaggg agaccaatag atcgatgctt ttctgtacag ccgcggccac gccaggtgta
     2581 ataccttggg caaagtattc cttgggcacc ccgatcttca ggcctttaac attcttgtct
     2641 aaatagctgg tataatctgg cgttgcttgt ttagcggatg ttgtatcacg gggatcatat
     2701 tgtgcaatgg cgttcatcac cattgcacag tcgcgcacat cttttgttaa cggcccgatt
     2761 tgatctagtg aggaagcaaa cgctattaag ccataacgag aaacacgtcc gtaggtcggc
     2821 ttcatgccta ccacgccaca gaaagaagct ggttgacgaa tagaaccacc tgtatctgag
     2881 ccgatggcaa ataaagcctc atcggcagcc acagccgctg ctgaaccacc actagagcct
     2941 ccaggtactc tagtcaagtc ccagggatta cgcgtcgtaa aataagtgga gttttccgtt
     3001 gaagagccca tggcaaattc atcaagattg gttttgccca gcataataca gcctgctttt
     3061 tgcaaaagtt caactaccgt agcatcatag ggcggcaaaa aattatgtaa catttttgag
     3121 gcacaagtag tttttacacc ctttgtacac atattatctt ttaaagccac aggcacacca
     3181 gccaaggggc ctacctgctc accattttta cgttttgcat caatttgggc agcagccgct
     3241 aaagcctgtt cgctagtgat agtaagataa gctttaactg tctcgtccac tgcggcgatg
     3301 cgctttaaag tcgcttctgt taactcctgc gcactgattt ctctagactg tagtttttct
     3361 acagcatcag caactgttaa ttgttccagc aagattatcc cccctactca attacgagcg
     3421 gcacacgaaa catgccatct tcagtttccg gagcattagc cagtgcttgt ccgtgcggca
     3481 gacagggttg ttctttatcc tcacgtaaaa cattgcgcag cggcaaaaca tgcgccgtgg
     3541 gttgaatatc cttggtatcc aactcattga gcttaccgat atattctaaa atcttattta
     3601 actcacctgt gaactgttgc atttcactaa cgttgatgtc cagtcttgct aaagcagcca
     3661 catgggcaac ttcttgatct gttattttca cttttgtacc tccttctaac cctgctcaaa
     3721 ggtaaaatac catgggcaaa ttataccata accaccactg ctcggcaact ttaacgacgt
     3781 tccgccctct ccaaaaacaa aaaaaagtac cacacttggg tacctctttt aatgaatagg
     3841 cattgttaaa gcaaattagc taaactgtga ccacgttagt ttgacagctt aacctgaaac
     3901 gaaaagccag aaacccttgt agcacaatgc tgcacgggta ttattttttt atattattgt
     3961 ctttaataag tataggaatg tatagtatta tatggtataa tataactatg taatcgaggc
     4021 ggaggtgaat aatttgaacc taaaacaatc acgcagaaaa aacggtagag tttatctttc
     4081 tatcgaaaaa agttatcgtg ataaggtgac tggcaagcca aaagctagaa cagttaaatc
     4141 tctcggctac ttggatgaat tagaaaagga ataccaagac cctatcgccc atttcaagga
     4201 aattgcccgc aagatgacgg aagaagaaaa caaaaggaaa aagttaacac taaccctcaa
     4261 tatggatgaa cagcttgcgc agggaacaga taacaggaaa aacctcggat atgccgcgat
     4321 cctgaaaatt tatcatgaac ttgggctgca ccgtttttta aataataaag cacgcaatga
     4381 agatttcaag ttcaacacaa actccattat gatgctgctt gtcgtctcca ggttactttc
     4441 ccccggctcc aagaaaaaga cattcgaaga gaaagaccgt tatttcgaac gcttcaactt
     4501 ttcgctgcca gatgtctatc gtgccctttc ccactatgct aagattgcta aagatctgca
     4561 gcaatttcta aatgagcaga tcacagaaaa atacgggcgt gacacaaaaa cggtttatta
     4621 tgatgtcaca aatttttatt tcgagatcga taagcctgat gaggtgcgaa aattcggccc
     4681 atccaaagag aagcgcccca atccgatcat acaaatgggt ctggcaatgg atgcaaacgg
     4741 gataccactg cactatgagc tttttcccgg gaacaagctg gataaggaaa cgtttcgctc
     4801 cgtcatcggt gaggttcgta aaaattacga cactggcaga atagtagtag ttgctgacat
     4861 ggggattatc acaggtgata atatttacta cctgaccggc gggaaaaaca gaaatgggta
     4921 cgtgttcagc ttttctgtcc gcggtggggc aaacgcattt aagaactatg tacttgataa
     4981 tgaggggtat gttggtactg acggcaaacc agctggagaa gatgccgaat tcaaaatcaa
     5041 gagcagacgc attgcgcgtg agatcaatgt aacaatgaaa agtggtaaaa aagcaaaaaa
     5101 aactgtctat gaaaagcaag tcgttttctg gtcaaagaag tatgcagtta aagcaaaggc
     5161 agaacgggaa gaacttgtga aaaaggcgtt agatctagta gcgagtccgc aaaagtataa
     5221 caaggctaca tcctacggtg cagccaaata cgtcaaacat ttggaattcg ataagaaaac
     5281 tggtgaaatt attgaaggaa agaaaaaacc atactttgat gctgataaac ttgcggaaca
     5341 ggaaatgtac gacggttatt acgctatcgt cacaagtgaa atgcagatga ctgatgggga
     5401 gatcattgat acataccggg gactatggga aattgaagaa accttcaaag tcacaaaagg
     5461 tacgcttgag gcacgcccgg tatacgtctc ccgtgaagat cgtatcgaag cacactatct
     5521 cacctgcttc atcgcccttg ttatcataag gcttattcag aagaaaatgg atcggcgcta
     5581 ttcgcccaaa atgattattg agtgcttaaa caaaattgcc tgctccaatg agcaggacaa
     5641 cctgtatctg tttgattaca gaagtgagat ctctgatgac atcggcaatg ttctcggtat
     5701 cgatttcacg cgaaaaagac ttcgcttggc tgatataaaa aatattttag caaactgcaa
     5761 aaaatagttg tgtcccatac atatttatgc aaaaacacga aggccgcgaa ccctttattt
     5821 acaggggttg cggcctttat ttcgcatttt ttgctgtcaa agtcaggata ctatatgcga
     5881 aacaacctca atatgtcaat acaaactgcg gcaagcagtt ttcctatcaa gcgtcgcatt
     5941 tgtctaggga ctggttgttc tgtgacaaag cagttacctt gactacgctg cctgcgtagg
     6001 cataacctcc taaggcgaag gggaacgaaa gtagcggtga gtggtaagca tctaaattaa
     6061 ggccagcatc aagattacaa tttccttgca tttaggcatc tgcactcctc gaaaggaaga
     6121 aacgagcagc attacacgaa actatgtcgg atacattaaa gtaaaaggag cagcttaaga
     6181 tgaaacataa acatcgctgg ttgaccgtga ccgggtatat gttattatta gttgtcattg
     6241 gccttgtatg gcccgctgaa gggcagtttg tgtcagccta cagacagatt ttaaaagcat
     6301 ccggtgttct tgtctcggat tactttgtca ttggcagtat ttccgccgct ctggtcaatg
     6361 ccgggttagt tggtctgtca ggcttggcct taatatattt cacccacact actctctcag
     6421 gccccaccat cgccgctgtc tttaccatgg cgggatttgc cttattcggc aaaactcctc
     6481 tcaatatctg gcccatcttc ctgggagtag cacttagtgc ctggctgaaa aaagagcagt
     6541 ttcgtaccta tatcgtcgtt gccctttttg gcagcgccct cggacctgtt gtcagccaag
     6601 tcgcctttgg tttaggactg ggatatctaa caggggctgt gatcggcctg ctcattggta
     6661 ttgttttgcc ggctttagcc ccgcatttac tgcagaatca ccaaggtttc agcctctata
     6721 atattggctt tacctgtggt atcattggca tgtttgtcgt agctctcctc aagctccgtg
     6781 gtctagaccc caccgcaaca atggtttggg ggtcagaaca atccaccgcc ttagctgcag
     6841 tctttttgct ttatttcttc tcaatgctga tcttaggctg gaaaggacgc cgaggtgccc
     6901 gtgagctctt aaagcttccc ggcactttag ttacagattt cgtttctgaa caaggttttc
     6961 gtgcctccct ttttaatatg ggactggtgg gtttaatggc tttagcttat attatcttgg
     7021 ttggcggtgc tgttaacggc cccactttag gtggtgtctt taccattgcc ggctttggcg
     7081 ccttcggcaa gcacccccgc aactgctggc cactcatggc cggtgtcttt atcggctccc
     7141 acttaagtgt ctggacggca gctgaaccgg gaccactgct ggcagcctta tttggcacta
     7201 cgctcgcacc aatcgctggc agcttcggcc cgttgtttgg tatggccgcc ggagttgtac
     7261 atctggctgt tgtaatgcac gtcggcattt tccacgcagg acttaacctc tacaataacg
     7321 gctttgcggg tggcctggta ggtacactct tcgtcagcct gagccgctgg tttaacttac
     7381 agagtaaaca cgattaaggg gggggcattg cgtggataaa gatcgcaaaa acatcactgg
     7441 cattgttgtc gagctcatga aaaatgcgct gcagaccaat gctacaagcg tgaacatatc
     7501 atttactgaa gcagatgaag aaataagaac cacgaacata ccgacgttcg tagttcttat
     7561 tctagtaaat attagctaac agattgctca tcacctgata ttttcatgtt gaaactcttc
     7621 ggctattttg gcgatgacct tcttttcaat gcgtgagaca taagagcggg aaatgcccag
     7681 ggttttggca atttcttttt gcgtttgcct gcgccccatg ggaaggccaa accgcagtgc
     7741 caagacggtg cgttcacgtt tttttagttt acgtattacc tcatacaact tggcttcaag
     7801 gagttttttg tcaacctgtt ccaacacttc ctcggcatcg gtaccaagga tatccaagag
     7861 cgttatttca ttaccctctt tatccgtacc aatcgggtca tgcagcagta cttctgcttt
     7921 tcttttggta atcacccgaa tatgcatgag gatttcattt tcgatacagc gggctgcata
     7981 agtggctaaa cgggtacctt tattggtctt ataagaatca atagccttaa tcaaaccaac
     8041 ggtaccaatg gaaatcaggt cctcctggtc ttcacctgta ttttcatatt tttttattac
     8101 atgtgctacc aagcgtagat tccgttcaat caggacactg cgggcttcgg gatcaccttc
     8161 agccaggcgc tgtaaataaa cctgttcttg ctgcggggaa aggggacggg gaaaagaatt
     8221 gttgtttaag taggaaacaa gtagggtaat ttcccggatc acagtcgcca cgattgtaat
     8281 gattggaatc aaaaaagcac ctccaccaag atgtcattat tgtattgtat gcacatggtg
     8341 gagtaattgt gtcaactgct aataatttgt tatttgttgc agttggcact acaacggaaa
     8401 tgaggcgtcc cctttcgtgt caacaaatga ttatggcaaa aatgaattgt ctttgcaagt
     8461 tttgaaataa tggttaagcg gttaagcagg aaacccgctt aaccgctatc aacattactg
     8521 tgagatatat attcattaat atccccttgt gttaggattg ttgtcatagg gctccgagta
     8581 aagtatagtt aatgtaagag gtctcggagg agattaagtc cgtataatgg tgtaaaaaca
     8641 aaatgagcca aaaactcatt cctcggttag aataagtacc agcaaatacc aaacagagga
     8701 ggatgaatca tggctcagtt taatattacc ctagccaaca cttcatctta tcccaacaac
     8761 acagataaag ccgtgccaat atatctttcc ttaataatcg tttgcatctg caccattaca
     8821 ggcatctgcc cctgcttttg tcttcactta ccccactcat cccagcggac aagttttgct
     8881 accaacctat catatgtcag aagcatctgg ctttccactt catctagctt ctttctatcc
     8941 ctttacttga ttacataagc tcccaatgta aagatcagta gagatgctaa catttacttt
     9001 gctttcttca tgataacctc caatagtttt tatttagcct atcttcggta taccgaccag
     9061 gttccagata gcctcttcta ccttcaaatt tgagtatatt aaaagtgaca tcttgcataa
     9121 ttaattgatt catttagtgt gtttagggta tggttctggt taacatctag cctgttaacg
     9181 caacagagaa caatgccacc atactcttta aattgatgca ccttatattg caccatctct
     9241 tttcttttct cgctgtcagt ttctggctca aaaacaatac ccagatccaa gacaataaaa
     9301 tcatagtcct ggcaagggat ctcctccaga agatttctta catcatcatg atataacttc
     9361 accgatccgc cattgctttg cttccacttt ctaaacgttt gtaggtagag aagaacaaag
     9421 ccatcgcctt cccaatagag atccggtcgc gacgccggcc acagaataga aataggctgt
     9481 tttgaaaggg atttagctga aagtctgctc aacgatagca gcaaggccgt caatggcttt
     9541 tcgcatatcg gtgtagccgc aagcaatata gattttctcg gctttggaga tatcaccgag
     9601 cattttgcag tgccttcaaa gtattctcaa tcaatgtcac agaggcattg ttgcgaagct
     9661 ctaatgtcac agatccaagc cgcagggtaa tcggtgaaga tgtttctgtt aggcctgtgc
     9721 cggctgcttc tattgaaatg ttgacaggca caatcgagct attctcccca ggggctggaa
     9781 gcgtttcgca ggctgcctca cgtactacgg tctgaccact gatacgacat tcggagataa
     9841 ttttagccca ttgattcaac caatattggt ctccagcacc tgtgggcggt ttgagacctg
     9901 cagctatatg ccgatctcga agggccttcc ttcatctttt gagaagcatc atttcagaca
     9961 gttcgttcct cctttcggtg tcttactttg ttcaggacgc acgcgggaat ccaggattca
    10021 aagaatgtag aaaaagaaga cccgctgata aaaaagcgga tcgtgagatt aattatcccg
    10081 acagataata tgggtataaa cctattgact acacccgaaa tgttgcttgt acagcaaata
    10141 tgagttgaat gtttaaagga aatcctaaat ttaataggtt tgtggttttg ctgcataaaa
    10201 aaactagtat aggtttaaaa ttagttttaa at
//
LOCUS       DASRMS010000147        18429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_239146, whole genome shotgun sequence.
ACCESSION   DASRMS010000147 DASRMS010000000
VERSION     DASRMS010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 18429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18429
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239146"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(374..1639)
                     /gene="murA"
                     /locus_tag="VFC74_06510"
     CDS             complement(374..1639)
                     /gene="murA"
                     /locus_tag="VFC74_06510"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZK25022.1"
                     /translation="MNGIISVEGGVPLNGRVRVEGAKNACLPIMAASLLCTGEVTLRE
                     IPGLEDVYTMCKVLNALGAKTDFDEEARALRVASGGELIAEAPYDLVRKMRASFLVIG
                     PLLARTGRARVSLPGGCAIGIRPIDLHLKGLKALGAEVIIGHGFIETVADKLRGERIY
                     LDFPSVGATENIMMAASLAEGITVIENAAEEPEVVDLARFINSMGGHVSGAGTDTIRV
                     HGVAALACTDYTVIPDRVEAGTFMLSAAITGGTVTVENVIPTHLTPLIAKLKEAGCEV
                     VEEEALIHIRRQGPIRAVDVKTLPYPGFPTDLQSPMMALLTYATGTSVITETVFENRF
                     MHVDELRRMGAQIKIESHSAVISGREQLCGAPVKATDLRAGAALVLAGLAAKGRTDVS
                     AIYHIDRGYWHFTEKLASLGAKITRSEQP"
     gene            complement(1757..2506)
                     /locus_tag="VFC74_06515"
     CDS             complement(1757..2506)
                     /locus_tag="VFC74_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwmB family TATA-box binding protein"
                     /protein_id="HZK25023.1"
                     /translation="MERKHKLALIFILLALAVVVYQLPGSPVADVEALAMVVETAGAA
                     VTEGEVQYFVVLNNRYQEMDELEAVLQETAALLGMQSPKIERSAGDTFRVVDATDKTA
                     FGPTAHLVVQSNPGDDHGIPAQTYLLVICRDTSVAALSAAVQQLTTTLQPYAPGGQLS
                     YYLSGQLADKKDRQEMEEMAKKALAAVRGTIVEGMQDEQLVSYTAYTPLVARYMTIDG
                     DRFNVNVAVRYDDYLQKTVVWAGFPLIHDPY"
     gene            complement(2701..3336)
                     /locus_tag="VFC74_06520"
     CDS             complement(2701..3336)
                     /locus_tag="VFC74_06520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003513511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HZK25024.1"
                     /translation="MEIQVNPTRMELNRLRGRLQMATRGHKLLKDKRDELMRQFVDII
                     KENKRLRESIEGKLADSFANFLLASAVMSSEALEEALMYPTASINVEVTNKNIMSVRV
                     PEITLVETADTAEHAANYGFAATSGELDTSIETLAQVLPELMRLAALEKKVHLLADEI
                     DKTRRRVNALEHVMIPELQDTIKYIRMKLDENERSTLTRLMKIKDIVRKQA"
     gene            complement(3340..4719)
                     /locus_tag="VFC74_06525"
     CDS             complement(3340..4719)
                     /locus_tag="VFC74_06525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HZK25025.1"
                     /translation="MLKEYRSVSEIVGPLMLIEHVEGVTYYELAEIEMKDGSVRQGRV
                     LEVDGDKALVQIFEGTSGLNVSESKVRFLGHGIELPVSMDMLGRVFDGLGRPRDNGPR
                     IIPEERRDIEGLPINPYARNYPSEFIQTGISTIDGLNTLVRGQKLPIFTGSGLPHNNL
                     AAQIARQAKVLGAEAKFAIVFAAMGITFEEADFFISDFRKTGAIDRSVLFINLADDPA
                     IERIATPRMALTAAEYLAYDKGMHVLVIMTDMTNYAEALREISAARKEVPGRRGYPGY
                     LYTDLATLYERAGRIRGVEGSITQIPILTMPEDDKTHPIPDLTGYITEGQIILSRSLH
                     RKGIYPPVDALPSLSRLKDKGIGQDKTREDHADVLNQLYAAYARGKEAEELAAILGEA
                     ALSDADKLFARFSHEFEARFVNQGEDEDRSILDTLTIGWELLTLLPRAELKRVRDEYL
                     DKYLPAEEE"
     gene            complement(4738..6507)
                     /locus_tag="VFC74_06530"
     CDS             complement(4738..6507)
                     /locus_tag="VFC74_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HZK25026.1"
                     /translation="MGRVIKVSGPLVVAENMQGANMYDVVRVSEKRLIGEVIEVRGDR
                     ASIQVYEETSGLGPGEPVYTTGIPLSVELGPGLIESIYDGIQRPLNVLYEQVGARLSR
                     GVEAHALDREKEWHFVPTAAVGQVVKPGYVLGTVQETALVEHKVMVPPGVEGTLTFLH
                     EGEATVAEMIAKVEQDGRTVEVAMLQNWPVRKGRPYREKLSPTEPLITGQRVIDMFFP
                     VAKGGTASIPGPFGSGKTVTQHQLAKWADAEIVVYVGCGERGNEMTDVLNEFPELKDP
                     QSGEPLMKRTVLIANTSDMPVAAREASIYTGITIAEYFRDMGYSVALMADSTSRWAEA
                     LREISGRLEEMPGEEGYPAYLGSRLAEFYERAGKVVCLGEDNRIGALTAVGAVSPPGG
                     DISEPVSQNTLRIVKVFWALDSSLAYRRHFPAINWLTSYSLYTNNFSEFYKAEAGSEW
                     IEMRVEGMRILQEESELEEIVRLVGVDALSEKERLTLETARSIQEDFLHQNSFHEVDT
                     YASLTKQFRMMKLIMLYHHEAQAAIEHGADFQALLKLPVREQIARSRYLAEDDLGAMD
                     KIEAAIREQIADLSGDDLEQTYA"
     gene            complement(6528..6860)
                     /locus_tag="VFC74_06535"
     CDS             complement(6528..6860)
                     /locus_tag="VFC74_06535"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003518476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HZK25027.1"
                     /translation="MSMYKVAVVGDRDSILGFKALGVTTYPVTSNEEAIRALQGIARE
                     NIAVVCITEAVAEQIQPQVAALQKKMLPAVVFIPNNKGSLGLGMAQIKKNAEKAIGAD
                     ILFGKEGS"
     gene            complement(6847..7833)
                     /locus_tag="VFC74_06540"
     CDS             complement(6847..7833)
                     /locus_tag="VFC74_06540"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit C"
                     /protein_id="HZK25028.1"
                     /translation="MSNSKYTYAVGRIRAMETRLLDAGKIDRMATAHDAEEALKVLGE
                     TEYAAHLADVENANYFEPILAAERRRVYLELRKMLPEPDLINLFAQQFDYHNLKVLLK
                     AHHLGEKRDELLEPELGNIPLDHLVVAVTAEDFSSLPTRMREAAEKIKAQFHPQLVDL
                     WLDHALYEELASEAQRHSPFISNYFTSLRDLTNIKTFIRVKRLNRTPEFLASALLPAG
                     ELDLLELLSLEEPLEELVAHLSKTRYASVVSEGVLNYQQTETLTRYEKLADDFIINYV
                     KQAKHYIMGPEPVVAYLLAKENELKMIRMIMVGKINQLPVEAIRERLRDVYV"
     gene            complement(7884..8489)
                     /locus_tag="VFC74_06545"
     CDS             complement(7884..8489)
                     /locus_tag="VFC74_06545"
                     /inference="COORDINATES: protein motif:HMM:NF014093.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E"
                     /protein_id="HZK25029.1"
                     /translation="MSGAEKLKEKIVADATSEAEDLLAGARERAAASRDKAEAEAAAK
                     KQKIEADVAKKAAELQRRDQTIAELDARKAILAAKEDLIEDTFKQALTRLQNLEPQAY
                     QAILFNMLLATASTGSEEIIISPADKVHFSPEFLARVNKALTQEGKQGNLQLSAETRE
                     LQGGFVLSAGDVEINSSFAALLRMQRDQLEPEVAAILFTNA"
     gene            complement(8503..8979)
                     /locus_tag="VFC74_06550"
     CDS             complement(8503..8979)
                     /locus_tag="VFC74_06550"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004850778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HZK25030.1"
                     /translation="MIGTGLLLAIVGAALAVILAGIGSAVSVGRVGQAAAGVVTEDPD
                     KFGPTLLLQALPGTQGIYGLLVGFLILQRTGLIGGTVATITMQEGLGLFFAALPIAIV
                     GLISALVQGQTAISGVHLVAKRPEEAGKAIIYAVMVETYAVLALLMSILMLFGIQV"
     gene            complement(9051..10997)
                     /locus_tag="VFC74_06555"
     CDS             complement(9051..10997)
                     /locus_tag="VFC74_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HZK25031.1"
                     /translation="MSKVTLLAHQTDKKNILELLQQSGVLEISDLTEQMAAEEAFADF
                     LEQDHEQEAMQAVEKSMAEVRFNLDFLNRYHRVNKGLLDDILVEKEVLTLKTVQEKVP
                     HWAAISSNVYQTLKAVDEKLMSLRNEETRLQNLQRQLQPWQQLTITLPEITNTKAVRL
                     ELGTVPVTQLKLIESQLAAAEAACYLEEIDSQRGEAYILLAYHHQHAELVQNALKQVN
                     FNRQQLPQLPGTVVENLRHIEQGLTTVTAERKTALAQIAEQVQHRQTLQYYLDYLTVQ
                     REKNQAVDNLARTDSTFVLQGWVPTAEVAQLQQRILALSETIGLTVSEPEEGENFPVK
                     LENKPFFAPFEFVTKLYGIPSPYGIDPTASFTAFFIIFFGFCLTDAGYGLVLMLLAGI
                     GLRKIKGENMRKLLKILFWGGLSTVVIGWLVGGWFSYQIIGAPLAFDALANPILLLGI
                     ALGIGVVHIFYGMGVKFVSLVRQGKTLDALFDVGLWYVLLTGLGLLALPSTAKIGKYM
                     ALAGAIGLILTQGRSQPTILKKFFSGLVSLYDITGYFSDILSYSRLLALGLATGVVGL
                     AINTMAGLLVGSPIGWIPMVILLIGGHTFNLAINGLGSFIHSSRLQYIEFYNRFYEGG
                     GRAFAPFRFNTRHMEIQQETTQHE"
     gene            complement(10999..11325)
                     /locus_tag="VFC74_06560"
     CDS             complement(10999..11325)
                     /locus_tag="VFC74_06560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25032.1"
                     /translation="MLGDVIKDIRAAEEQAAALKAEAQTEARRIVREAEVAAVETVEQ
                     TTTEAEAAGRQRLAVLEEERESLIAPIRERTAAEIKVMLAAAVTQQEAAITMVMERIV
                     KSYGHS"
     gene            complement(11392..11592)
                     /locus_tag="VFC74_06565"
     CDS             complement(11392..11592)
                     /locus_tag="VFC74_06565"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HZK25033.1"
                     /translation="MKREDFTALRVVIELALSISCSLLGGVYLGRFLGQRFGHPSLYL
                     LAGILLGLLAGFGQAKRILLKK"
     gene            complement(11836..12297)
                     /locus_tag="VFC74_06570"
     CDS             complement(11836..12297)
                     /locus_tag="VFC74_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HZK25034.1"
                     /translation="MRPAWDNYFMEIAHMVATRSTCLRRAVGAVIIKDKRILTTGYNG
                     APSGLAHCEEVGCLRDQQQVPSGQRHELCRGLHAEQNAILQAALYGVPIGGATLYCTT
                     HPCVVCAKMIINASIKEVVITDSYPDKMSAELLAEAGITVRYLEYKQGSKL"
     gene            complement(12317..12949)
                     /gene="upp"
                     /locus_tag="VFC74_06575"
     CDS             complement(12317..12949)
                     /gene="upp"
                     /locus_tag="VFC74_06575"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HZK25035.1"
                     /translation="MNNKQTFIFNHPLVEDKVARLRDRNTNVALFRQLVEEVTVLMLY
                     EVSKSLPCTTKEVETPLATTACQVLDGPAPVLVPILRAGLAMADPLLKLLPNSPVGHV
                     GLARDHETLAPVRYLVKLPPLADRAVWVLDPMLATGGSASATLQILKDHGANNLTLFA
                     IIAAPEGVAHLEKAHPDVKLFIAKLDERLNEKGYILPGLGDAGDRLYGTL"
     gene            complement(12942..14177)
                     /gene="glyA"
                     /locus_tag="VFC74_06580"
     CDS             complement(12942..14177)
                     /gene="glyA"
                     /locus_tag="VFC74_06580"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HZK25036.1"
                     /translation="MSTLQLIDAEIARVIALEKQRQQDHLELIASENYVSRAVLEAQG
                     SVLTNKYAEGYPSRRYYGGCDYVDEAEELARTRAQELFGAEHANVQPHSGASANLAVY
                     LAALKVGDTMLGMNLAHGGHLTHGAPVSLSGKYFHAVSYGVDRSTGLLDYNQVEDLAK
                     KHKPRLIVAGASAYPRIIDFTAFRQIADRVGAYLMVDIAHIAGLVAAGIHPSPLPVAE
                     FVTSTTHKTLRGPRGGLIMCRQEYAAAIDKAIFPGLQGGPLMHVIAAKAVCFKEAQLP
                     EYAVYVQQVVTNARVLAKCLLNEGFDLVSGGTDNHLMLVDLRSQGLNGKEAEEMLGEV
                     GITINKNTIPFDPANSHVTSGIRIGTPAVTSRGMKEPEMELIAELISATLQHKGAEQV
                     KTQVRHRVKELCASFPIYE"
     gene            complement(14204..14659)
                     /gene="rpiB"
                     /locus_tag="VFC74_06585"
     CDS             complement(14204..14659)
                     /gene="rpiB"
                     /locus_tag="VFC74_06585"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008676598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HZK25037.1"
                     /translation="MKIAIASDHGGFHLKQQINTYLAEEGYISHDLGTMSAASVDYPD
                     FALQVAEAVAGGEYNLGIICCGTGIGVSIAANKVPGIRAAHCHDTFSARMSREHNDAN
                     VLTLGERVLGPGLALEVVAAFLQGSYAGGRHACRVEKIKAIEHKYCRQG"
     gene            complement(14656..15186)
                     /locus_tag="VFC74_06590"
     CDS             complement(14656..15186)
                     /locus_tag="VFC74_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HZK25038.1"
                     /translation="MRKISKQQKLLLFVCTGNTCRSPLAQVMAAAQLAQADLSDWRVA
                     SAGLAALKGMSASKHALTVANALQLDLSVHQSQPFTAALAAQADLILVMTDTHQESIL
                     QQQPQLKGKVSTIREFSGSTGNVQDPFGGTAAEYLATAQELESLLQTIVKKLAAAGVK
                     KQDEGKQSRINQGGCL"
     gene            complement(15183..16253)
                     /locus_tag="VFC74_06595"
     CDS             complement(15183..16253)
                     /locus_tag="VFC74_06595"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HZK25039.1"
                     /translation="MLRETIKGTLIFDGITPATLSREAAAAAQILRQGGLVAFPTETV
                     YGLGGNALQPQVVAKIFRAKGRPMDNPLIVHIHEASQMSDLAAEVPAEAWLLAKHFWP
                     GPLTLVLQKQDHVPRETTGGLDSVAIRLPAHPTALALLRAAAIPLAGPSANLSGRPSP
                     TTAAHVLQDLAGRIEGVLDGGPSKVGVESTVLDIRGGSPRILRPGGITAQQISTVLQR
                     DCPVASWVQTSGEAPPSPGLKYLHYAPQAPLYLVKSHNAGQLLAKLRQLQQDWQQQGK
                     RVGMLVSQESAAQLQGDDIIVLGSRQQPADLASHLFAALREFDQRQVEVIVAEGYGAA
                     GVGLALMNRLEKAAGPRVIEVG"
     gene            16567..17331
                     /gene="larB"
                     /locus_tag="VFC74_06600"
     CDS             16567..17331
                     /gene="larB"
                     /locus_tag="VFC74_06600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004621578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HZK25040.1"
                     /translation="MDKKQLHSLLSQVQNGTVAVADALQRLRNFDYEDLGFAKVDHHR
                     ALRQGFPEVIFCPHKTTAQIVSIAARLAQNNTTVLATRAAAHIFEALQASLPQVEYHE
                     LPQVVVINRDHNLPAVGQVLIVSAGTADLAVAEEAAITAELMGSQVNRLYDVGVAGIH
                     RLLANQAQLQAANVLVVVAGMEGALASVVGGLVDKPIIAVPTSVGYGASFGGVAALLS
                     MLNTCASGVSVVNIDNGFGGGYTAALINRLAAQEES"
     gene            17334..>18429
                     /gene="larC"
                     /locus_tag="VFC74_06605"
     CDS             17334..>18429
                     /gene="larC"
                     /locus_tag="VFC74_06605"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HZK25041.1"
                     /translation="MKLLYFDCFAGASGDMILGALLAAGATLDFVNSELAKLPFTGYQ
                     LKTTQVIKKGIAATRLLVEITEAQQPHRSYHTIREMLNQSDLAKPVRDLSLAIFARLA
                     AAEGKIHGRSPEEVHFHEVGAVDSIIDIVGAAAALTELAADKVYASALHTGRGFVNCA
                     HGRLPVPAPATLEVLAGAPIYATELEGELLTPTGAAILATIAEFTPLPLMTVQQIGYG
                     AGQKELPIANVLRVMVGESADSDTAQHEQVTVLEATIDDQNPELYGYIMAKLFAMGAV
                     DVTMTPLYMKKNRPGILLTVLVAAAQEQAVITTIFQETTTLGLRRSTAEKLRLTRRQL
                     TVQTPYGSVRMKIAEQAGQICNAAPEYADCT"
BASE COUNT         4679 a         4825 c         4155 g         4770 t
ORIGIN      
        1 atcctattgt ggctccgaat aagagtaaac atgacttcaa aaaagactgt ttaacacatc
       61 atttttatta taatgtgtaa ttttcttgta ttaatacatt tcacgcccaa atcattacag
      121 tattgcttat attctaccac atttatcagc ttcctgctca aaaagagagt tttttcttta
      181 aaagttaaca aagttgacat aatctttcgc aatttgcatg tgcatttagc tgcataatta
      241 ggaaggagga taactataca agcttttaca ataagtaagt gattaccttg cacttcaata
      301 tattcagaat gaacttttct gttcatataa taatatcagc agaatactta tattcacaaa
      361 aagaaagcag cccttaaggc tgctcactgc gtgtgatttt ggcgcctaaa ctggcgagtt
      421 tttcggtaaa atgccagtag ccgcggtcta tatggtagat ggcactgaca tcggtgcgcc
      481 ctttcgcggc taaaccagcg aggacaaggg cagcaccagc acgtaaatca gttgctttaa
      541 cgggagcgcc acacagctgt tcgcgtccgg agatcaccgc actatggctt tcaattttaa
      601 tctgtgctcc catgcggcgc agctcatcta catgcataaa acggttttca aacactgtct
      661 ccgtaattac tgaagtacct gtagcataag tcaatagtgc catcatcggt gactgtaagt
      721 cagtgggaaa accaggataa ggtaatgttt taacatccac agccctaatg gggccttgac
      781 gacgaatatg aataagagct tcttcttcca ccacttcaca gccagcctct tttaatttag
      841 caattaaagg cgtcaagtga gtaggaataa cgttttccac ggttacagtg ccgccggtaa
      901 tggcggcgct taacataaaa gtaccggctt ctacgcggtc tggaataaca gtataatctg
      961 tacaagcaag cgcagcaacg ccatggacac gaatagtatc tgtaccggca ccactgacat
     1021 gaccgcccat ggagttaata aaacgtgcca aatcaactac ttccggttcc tcggcggcat
     1081 tttcaatgac ggtgatacct tcggctaaac tggcggccat cattatattt tctgtagccc
     1141 ccacactggg gaagtctagg tagatccgtt caccacgcag cttgtccgcg acagtttcaa
     1201 taaacccatg accaataatc acttccgccc ccaaggcttt caggcctttg aggtgcaaat
     1261 caataggacg aataccaatc gcacagccgc cgggcaggga aacgcgggcc cgaccggtgc
     1321 gtgctaacag tgggccaatc actaaaaagg aagcgcgcat tttgcgaacc aagtcataag
     1381 gtgcttctgc gatgagctct cctcctgatg ctacccgcag tgctcttgct tcttcatcaa
     1441 aatctgtctt ggcacccaaa gcgtttaaga ccttacacat cgtataaacg tcttccagcc
     1501 caggtatttc tcgcaaggtg acctcccctg tgcatagcaa tgaggcggcc attatgggca
     1561 gacaggcatt ttttgcacct tcgacacgta cacgcccgtt tagcggtacg ccgccttcaa
     1621 ctgaaataat accattcaca tactgtggcc ccctcatctc tagttacaaa gatcaattta
     1681 gcctgctttt ctttttatct tttgaagaga ttgccgtctt tatactgctg tgctgcagaa
     1741 aatttaaaac cgggccttag taaggatcgt ggatgagagg aaaaccggcc caaactactg
     1801 ttttttgcag atagtcatca taacggacgg ccacattgac attaaagcgg tcaccatcaa
     1861 tcgtcatata acgtgctacc agcggcgtgt aagcggtgta gctaactaac tgctcatcct
     1921 gcatgccttc tactatcgtg ccccgcactg cggccagggc cttcttcgcc atctcctcca
     1981 tttcctgacg atcttttttg tcggcaagct gtccgcttaa ataatagctg agctggcccc
     2041 ccggcgcata gggctgcaga gtggttgtca gctgctgcac agcagcagaa agtgctgcta
     2101 cagaagtatc acggcagatg accaagagat aagtttgcgc tgggatacca tggtcatcac
     2161 ccggattact ttgcactacc agatgagcag tagggccaaa agcagttttg tccgtcgcat
     2221 ctacgacacg aaaagtatcc cctgcacttc tctcgatttt gggactttgc atgcctagta
     2281 acgccgccgt ttcctgcaag actgcttcca gctcgtccat ttcctgatag cgattattga
     2341 gcacgacaaa atattgtact tcgccttcag taacggccgc cccggctgtt tccaccacca
     2401 tggccaaagc ctcaacatct gccacggggc tcccgggcag ctgataaaca actaccgcca
     2461 acgctaacaa aataaaaatg agtgcgagct tgtgcttacg ctccattacc tcaccctcct
     2521 ctactctata gttttggcat atttttcccc ttttaaacct gccaactaaa aaaaccttct
     2581 gtactcaaca gaaggttttt taaggttcgg gtgaacacag ttcaccgctg cagttttagc
     2641 tggtctagct agctggtaaa agtctccccc ccaccagcag tgaataaaaa caaactgacc
     2701 ctaagcttgc ttgcggacga tgtctttgat tttcatgagg cgggtgagcg tgctgcgttc
     2761 attttcgtcc agtttcatgc ggatatattt aatggtatcc tgcagctccg gaatcatcac
     2821 atgttcgaga gcattaacgc gacggcgtgt tttgtcaatt tcatcagcca gcaggtgaac
     2881 ttttttctcc agcgcagcca ggcgcatcag ttcaggcagc acttgggcta aagtttcaat
     2941 ggaagtatcg agttcaccgg aggtggcggc aaaaccataa ttggccgcat gctctgcagt
     3001 atctgctgtt tccactagcg taatttcagg aacacgcaca ctcataatgt ttttgttggt
     3061 cacctcaacg ttaatggaag cagtagggta catcagggct tcttccagcg cctcactgct
     3121 catcacggca ctggctaaaa ggaagttagc aaaagagtct gctagttttc cttcaatgct
     3181 ttcccgcagt cgtttgtttt ctttgatgat atcgacaaat tgacgcatta attcgtcacg
     3241 tttatctttt agcagtttat gaccacgtgt agccatctgc agacggccgc ggaggcggtt
     3301 taattccatc cgggttggat tgacttgaat ctccatcccc tactcctcct cggccggcag
     3361 gtacttatcc aggtattcat cacgtacccg ctttaactcg gcacggggca gcagtgtcag
     3421 cagctcccag ccgatagtca gggtgtcaag aatactgcga tcctcatctt cgccttggtt
     3481 gacaaaacgt gcttcaaatt catgggagaa gcgtgcaaag agtttatcag catcagaaag
     3541 ggctgcttca ccgagaatag cggccagctc ttccgcttct ttaccacggg cgtaagccgc
     3601 atagagttga tttaagacat cggcatgatc ttcccgggtt ttatcttgac caatcccttt
     3661 atctttcaga cgagaaagag aaggcagagc atctacagga ggataaattc ctttgcggtg
     3721 cagcgaacgg cttaagataa tctgtccttc cgtgatatag ccagttaagt cggggatggg
     3781 gtgtgtctta tcatcttcag gcatggtgag gatgggaatt tgggtgatgg agccttccac
     3841 accacgaatc cggccggcgc gctcataaag ggtggccaag tcggtataaa gatagccggg
     3901 ataaccacgg cgcccgggaa cttctttacg ggcggcagaa atttctcgca gggcttcggc
     3961 gtagttggtc atatcagtca taatcaccag cacatgcata cctttatcat aagctaaata
     4021 ttcggcagcg gtgagagcca tgcggggggt ggcaatccgt tcaatggctg ggtcatcagc
     4081 caagttaata aagagcactg aacggtcgat cgcaccggtt ttgcggaagt cagaaataaa
     4141 gaaatctgct tcctcaaaag taatacccat cgccgcaaag acaatggcga atttggcttc
     4201 tgcaccaagt actttagcct gccgagctat ttgggcggcc aggttattat gtggtaaacc
     4261 tgaacccgtg aaaataggca gcttttgtcc ccggactaaa gtatttaaac cgtcaatcgt
     4321 ggaaataccc gtttgaataa attcggaagg gtaattgcgg gcgtagggat tgatcggcag
     4381 tccctcaata tcccgacgct cctccggtat aattctggga ccgttatcac gggggcggcc
     4441 tagaccgtca aaaacgcggc ctagcatatc catagaaact ggtagttcga tgccgtgacc
     4501 gaggaaacgt accttggact cactgacgtt cagtccgctg gtgccttcaa agatctgcac
     4561 caaggcttta tcaccgtcaa cttccagcac acgtccctgg cgcactgaac cgtccttcat
     4621 ttcaatttca gccagttcgt aataggtcac gccttctaca tgttcaatca gcatcaaggg
     4681 tccgacaatt tctgaaacgc tacggtattc ttttagcaat aacctctcct ccttccctta
     4741 cgcgtatgtc tgctcaaggt catcaccact taaatcggca atttgttccc tgatcgcagc
     4801 ttcaatttta tccatggcac ctaaatcatc ttccgctaga taacgggagc gagcaatttg
     4861 ttcacgtaca gggagtttga gtagggcctg aaaatcagca ccgtgctcaa tggctgcttg
     4921 tgcttcatgg tgataaagca taatcagctt catcatccgg aactgcttgg tcagtgaggc
     4981 ataagtatcc acctcgtgga aagagttctg atgcaaaaag tcttcttgaa tagagcgagc
     5041 tgtttccagc gtcaaacgct ctttttccga gagggcatca acaccgacta aacgcacaat
     5101 ttcctccagc tcggattctt cctgcagaat acgcatacct tccacgcgca tctctatcca
     5161 ttcgctgccg gcttctgctt tatagaattc actgaagtta ttcgtataca gtgaataaga
     5221 agtcaaccag ttaatggccg ggaagtggcg gcggtaggct aaagaagagt ctagcgccca
     5281 gaatacttta acaatcctta aggtgttctg ggaaaccggt tcagagatat caccaccggg
     5341 cggggagaca gcaccaactg ccgtcagagc accgatgcga ttatcttcgc ctaaacaaac
     5401 cactttgccc gcccgctcgt agaattccgc taaacgagag cctaggtagg caggatatcc
     5461 ttcttcacca ggcatttctt ccaagcggcc ggagatttcg cgcaaagctt cggcccagcg
     5521 cgaagtggag tctgccatca gcgctacgct gtaacccatg tcacggaaat attccgcaat
     5581 ggtaatccca gtataaatag atgcttcccg ggcggccacc ggcatatcag aagtattagc
     5641 aattaagaca gtacgcttca tcagcggttc gccgctttgg gggtctttta attccgggaa
     5701 ttcatttaaa acatcagtca tttcattgcc acgttcaccg cagccgacat aaaccacaat
     5761 ctcagcatct gcccattttg ccaactggtg ctgggtaaca gttttaccgg agccaaacgg
     5821 gccgggaata ctagccgtac ctcctttggc tacagggaag aacatatcaa ttaccctttg
     5881 accggtaatt aacggttccg tgggagaaag cttttcacga tagggacggc ctttacgtac
     5941 tggccaattc tgtagcatgg caacctcaac tgtgcggccg tcttgttcta ctttggcaat
     6001 catctccgcc actgttgctt caccttcatg caaaaacgtt agcgtcccct ccacaccggg
     6061 tggtaccata actttatgtt ctaccagcgc agtttcctgt actgtaccta aaacataccc
     6121 gggtttaacc acttggccta cggccgcggt cggtacaaag tgccactctt tctcccggtc
     6181 taaagcatgg gcttccacgc cccggctgag gcgggccccg acctgctcat aaagcacgtt
     6241 taaggggcgt tgaataccat cataaataga ttcaatcaga ccagggccca attccacact
     6301 taaggggatg ccagtagtat aaaccggctc tccaggaccc agtccgcttg tttcttcata
     6361 aacttggatg gaagcacggt cgccccgcac ttcaataact tcaccaatta ggcgcttctc
     6421 gctcacacgc acaacatcat acatattggc gccttgcata ttctcggcta cgacaagtgg
     6481 gccggatact ttgatcaccc tacccacatt ttctgtattg gtcaaagcta actaccctct
     6541 ttcccaaata agatatcagc gccaatggct ttttctgcat tcttttttat ctgggccata
     6601 cctaacccta aactaccctt attgttaggg atgaacacca ccgcggggag catttttttc
     6661 tgcagtgctg caacctgcgg ttgaatttgc tcggccacgg cttctgtaat gcagacaact
     6721 gcaatgttct ctctggcaat gccttgcaga gcacggattg cctcctcatt gctagtaacc
     6781 gggtaggtgg tgacaccaag cgctttaaag cccaaaatgg aatctctatc tccgacaacg
     6841 gcgaccttat acatagacat cacgtagcct ttctctgatc gcttccacag gcaactgatt
     6901 aatttttcct accatgatca tacgaatcat ttttagttca ttttccttgg ctaaaagata
     6961 cgctacaacc ggttcaggtc ccatgatata atgtttggcc tgcttgacgt aattaatgat
     7021 aaaatcatcg gctaatttct cgtatcgtgt taaggtctca gtttgctgat aatttaaaac
     7081 accttcgctg accacggacg catagcgcgt cttggacagg tgggccacca actcctctag
     7141 cggctcttcc agggagagca gctccagcaa atccagctcc ccagcgggca gcagggcact
     7201 ggccagaaat tccggtgtac ggtttaaacg tttgacgcgt ataaaggtct tgatattcgt
     7261 gaggtccctg agagacgtaa agtagttact aataaaggga gaatggcgtt gagcttcgct
     7321 ggccagctcc tcgtaaagag catgatccaa ccataaatcc actagctgag gatgaaactg
     7381 tgccttgatt ttttctgccg cctcacgcat acgcgttggt agtgaactaa aatcttctgc
     7441 tgtcacggcc accactaagt ggtcaagggg gatattaccc aattccggtt ctaataactc
     7501 atcacgcttt tcacccaagt ggtgtgcttt cagcaaaact tttaagttat gataatcaaa
     7561 ctgctgcgca aataaattga tcaggtcagg ctcgggcagc attttacgca attccaggta
     7621 aacgcgtcgt cgctctgctg ccaagattgg ttcgaaataa ttggcatttt ccacatctgc
     7681 caaatgtgcc gcatattctg tttccccgag cacttttaag gcttcctcag catcatgggc
     7741 tgtggccatc cggtcaattt ttcccgcatc taatagtctc gtttccatag cacgtatgcg
     7801 gcccacggca taagtatatt tgctattcga catgctccaa gcctccttta cactggaggt
     7861 taaaaatgtc caacctcaag agattatgca ttagtaaata aaatggccgc cacttctggc
     7921 tctagctgat cccgctgcat acgtaatagg gcggcaaagg aactgttaat ttccacatcg
     7981 ccggcactta agacaaagcc accctgcagt tcgcgtgttt ccgcagacaa ctgtaggttc
     8041 ccttgtttgc cctcttgcgt cagggctttg ttaactcgtg ccagaaattc cggtgaaaaa
     8101 tgcactttat ccgccggaga aatgataatt tcttcgctgc cagtagaggc agtggccagg
     8161 agcatgttaa agagtatggc ctgataagct tgtggctcca gattctgcag tcgtgttagc
     8221 gcctgcttaa aggtatcttc aattaaatcc tctttggcgg ccagaatagc tttacgggcg
     8281 tccagttcag caatagtttg gtcacgacgc tgtagttcgg cagctttttt ggccacatct
     8341 gcctcaattt tttgcttttt agcggcagcc tcggcctctg ccttgtccct gctggcagcg
     8401 gctcgctcac gagcgccagc cagcagatct tctgcttcgc tagtggcatc tgccacaatt
     8461 ttttctttta gtttttcggc tcccgacatt aggttgccct ccttagacct ggatcccaaa
     8521 tagcattaag atcgacatta atagggctaa caccgcgtag gtttcaacca ttacagcata
     8581 gataatcgct ttaccagctt cttcagggcg tttagcaact aaatgcacac cactgatagc
     8641 tgtttgacct tggaccaaag cagaaattaa accaacgatg gcgatgggca gggcggcaaa
     8701 aaacaagcct aaaccttctt gcatcgtgat ggtcgcgact gtaccaccaa tcagtccagt
     8761 acgctgtaaa attaagaaac ctaccaaaag tccatagatc ccctgtgtac caggcagcgc
     8821 ttgcaaaagg agcgtaggtc caaatttatc aggatcttct gtaacaacac ccgctgcagc
     8881 ctgacccaca cggcccacac ttacagctga accaatacct gccagaatta cagccagggc
     8941 agcaccaaca atcgctaaaa gtaaaccggt tcctatcatg actatttatc ctcctttttt
     9001 tcaggtcctc ttgtcaacct gttgccaata attttgctga cgataacaag ttattcgtgt
     9061 tgagttgttt cctgctgaat ctccatatgc cgagtattaa agcggaaggg tgcaaaggca
     9121 cgaccgccac cctcataaaa gcgattatag aactcaatgt actgcaggcg gctggagtgg
     9181 ataaaagaac ccaaaccatt aatggctaaa ttaaaggtat gaccgcctat taataaaatt
     9241 accatgggaa tccagccaat aggactgcca accagcaatc cagccatcgt gttaatggcc
     9301 agcccgacca cgcctgtagc cagacctaga gccagcagac gtgagtaaga caagatatca
     9361 ctaaagtagc cggtaatatc atacaaggag actagccctg agaaaaactt cttaagaatc
     9421 gtaggctgac tgcgcccttg cgtcaaaatt aaaccaatag caccagccaa agccatatat
     9481 ttaccaatct tagcggtact gggcaatgct agtaaaccta aaccagtcag caggacatac
     9541 cacagcccca catcaaacag ggcatcaagg gttttccctt gccgtaccag gctgacaaac
     9601 tttacaccca tgccgtagaa gatatgcacc acacctatcc ccaaggcaat gccaagtaag
     9661 aggatggggt tagctaaagc atcaaaggcc agtggggcac caataatctg gtaactaaac
     9721 caaccgccta ccagccagcc aatcacgact gtggataagc cgccccaaaa gaggattttc
     9781 aatagcttac gcatattctc cccttttatt ttccgtaaac ctatcccggc cagcagcatt
     9841 aaaaccagac catagccggc atcggttaag cagaaaccga aaaatatgat aaaaaaagcg
     9901 gtgaaagaag cggtgggatc aatcccatag gggctgggga taccatatag ttttgtgaca
     9961 aactcaaaag gagcaaagaa tggcttgttc tccagcttta cggggaagtt ttcgccttcc
    10021 tccggttcac ttacggtcag cccaatggtc tcacttaaag caagaattct ctgctgcagc
    10081 tgcgccacct ctgccgttgg tacccagcct tgcagcacaa aggtactgtc ggtacgtgct
    10141 aaattatcaa ctgcctgatt tttctccctt tgtaccgtga ggtaatctag ataatattgt
    10201 aaagtttggc ggtgctgcac ctgctccgca atctgggcga gggccgtttt ccgctctgca
    10261 gtcaccgtag tcagcccctg ctctatgtgc cttaaatttt ccaccactgt acctggcagc
    10321 tgcggcagct gctggcggtt aaagttaacc tgttttaggg cgttttgcac cagctcggca
    10381 tgctgatggt gataagccaa gagaatgtat gcttcaccac gctggctgtc tatttcctct
    10441 aggtagcagg cagcctcagc tgctgctagt tgagactcaa tcagctttaa ttgggtgacc
    10501 ggtacagtac ctaactccag acgtaccgct ttagtatttg tgatttctgg cagggtaata
    10561 gttaattgct gccacggctg cagttgtcgc tgcaagtttt gcaggcgggt ttcctcatta
    10621 cgtaagctca ttaatttctc atcaacagct tttaatgttt gataaacatt gctgctgatt
    10681 gctgcccagt gcggtacctt ttcctgcact gttttaagcg tgagcacttc tttttctacc
    10741 aggatatcat ctaaaagccc cttattaaca cgatgataac gattaaggaa atctaaatta
    10801 aaacgaactt ctgccatgct tttttccaca gcttgcatgg cttcctgctc atgatcttgt
    10861 tccagaaagt cagcaaaagc ttcctctgcc gccatctgct cggtgaggtc gctgatttcc
    10921 agcacaccgg actgctgcaa gagctctaaa atattcttct tatcggtctg gtgggcgagc
    10981 agggtaacct tactcatctt agctatgccc atagcttttc acaatcctct ccatgaccat
    11041 tgttattgct gcttcctgtt gtgttacggc tgcagccagc ataactttaa tttcagctgc
    11101 agtacgctca cgtatgggag cgatcaaact ttcccgttct tcctccagta cagccagacg
    11161 ctggcggcct gctgcctctg cttcggtagt agtttgctct acagtttcca ctgctgccac
    11221 ttctgcttcc ctgacgatac gacgggcctc cgtctgcgct tcagctttca gtgcggctgc
    11281 ttgctcctca gctgcccgta tatctttgat cacatcccct aacaaataat ttcacctccc
    11341 ttttacagta ttgtctgcta atatatcacg atgattgtcg tagctttact tttatttttt
    11401 taagagaatc cttttggctt gcccaaagcc agccaagaga cctaagagga taccggccag
    11461 caaataaagg ctagggtgac caaaacgctg ccccagaaag cggcctaaat aaacgccgcc
    11521 cagcaaggag catgagatac tcaatgccag ctctatcacc acccgtaagg cagtaaaatc
    11581 ctcccttttc accggaccac ctctcaggct cacgtttaca ttccccagtt tttcactggc
    11641 agatgcgcca gttagttaca ctgcgtgtac gtgtccgtta aattagcttt tcgacacctt
    11701 aacttacatc tccttttttg cccacagtag tcgcaatcgt ggttcttgtg aaaatattcc
    11761 caaatattac agagacttta cattgtcaat aaattgcaat gtttggacaa aaaagtgctg
    11821 gtgtacatag cttctttaca atttactccc ttgtttatat tcaagatagc ggaccgttat
    11881 tccagcttcc gctagcagct cagcagacat tttatctggg taactatccg taatcaccac
    11941 ttctttgatg ctggcattaa taatcatctt ggcacaaacc acacaggggt gggttgtaca
    12001 atagagcgtg gcgccaccaa taggcacacc ataaagcgca gcctgtaaaa tagcattttg
    12061 ttcggcgtgc agaccacggc acagttcatg acgttgaccg gagggcacct gctgctggtc
    12121 acgcagacaa ccaacttctt cacaatgagc taaaccactg ggagccccgt tataaccggt
    12181 agttaagatt cttttatctt taataatcac tgcacctacg gcgcgccgta aacatgtaga
    12241 gcgggtcgct accatgtgtg caatctccat aaaataatta tcccaagccg ggcgcatcgc
    12301 tcttcctccc ctttacttac aatgtgccat acaatcggtc gccggcgtcg cctagtcctg
    12361 gcaggatata gcctttttcg tttagtcttt catctagctt ggcaataaaa agtttcacat
    12421 caggatgtgc tttttctaga tgggccacgc cttccggagc agcaataatc gcaaagagcg
    12481 tcaaattgtt agcaccatgg tctttaagaa tctgtaaagt agcactggca gaaccaccag
    12541 tagccaacat ggggtctaaa acccagaccg cccggtcagc caggggaggt aatttgacta
    12601 aataccgtac aggtgctaaa gtttcatgat cccgggctaa ccccacatgc cccacgggcg
    12661 aattaggcag tagttttaac aacggatcag ccatggccaa gcctgcccgt aagatgggca
    12721 ctaagactgg tgccgggccg tctaaaacct ggcaggcagt ggtggccagt ggagtttcca
    12781 cttccttggt tgtacagggc aaagacttgc ttacttcata aagcatcagt acagttactt
    12841 cttctactaa ctgccgaaac aaagcgacat tagtgttacg gtcacgcaat ctggctactt
    12901 tatcttccac caaagggtgg ttaaaaataa atgtttgctt attattcata aatggggaaa
    12961 gacgcacaga gctcctttac gcggtgacga acctgcgtct taacctgctc agctccttta
    13021 tgctgtaatg ttgctgaaat taactcagca ataagttcca tttccggttc tttcatgcca
    13081 cgtgaagtaa ccgcaggcgt accaatacga ataccactgg tgacatgaga gttagctggg
    13141 tcaaaaggaa tagtattttt attgatggtg atccctacct cacccagcat ctcttctgcc
    13201 tctttgccgt tgaggccttg agagcgcaga tcaactaaca ttaagtgatt atctgtgccg
    13261 cctgagacca aatcaaaacc ctcatttaac aggcatttag ccaaaacccg ggcattggtc
    13321 acgacttgct gtacatagac agcatattca ggcagctggg cctctttgaa gcaaactgct
    13381 ttagccgcaa ttacatgcat cagcggtcct ccctgtaagc cggggaagat agccttatcg
    13441 atggccgccg catattcctg acggcacata atcagaccgc cgcgcgggcc gcgcaaagtt
    13501 ttgtgcgtgg tcgaagtgac aaactcggct accggcagcg gtgagggatg gataccagct
    13561 gccaccagtc ctgcaatgtg ggcaatatcc accattaaat aggcaccgac acgatcagca
    13621 atctgccgaa aggcggtaaa atcaatgatc cggggatagg cactggctcc ggctacaatt
    13681 agacgcggtt tatgtttttt agctaaatct tctacttgat tataatcaag taaaccggtg
    13741 ctgcggtcca caccgtagga gacggcatga aaatatttgc ccgacagact gacgggggca
    13801 ccatgtgtca aatgtccccc atgggctaaa ttcatgccca gcatagtgtc accaacttta
    13861 agcgccgcta aatagacggc tagatttgcg ctggcaccag aatgaggctg cacattggca
    13921 tgttctgcac cgaaaagttc ttgagcacgt gtacgggcta attcttccgc ttcatccaca
    13981 tagtcacagc cgccgtagta acgtcgacta ggatagcctt cagcatattt attggttaac
    14041 accgaccctt gggcttccag gactgcacgg ctgacatagt tttccgaggc aattaactcc
    14101 aaatgatctt gctggcgctg cttttctagg gcaataaccc gggctatttc cgcatcaatt
    14161 aattgtagcg tcgacatctg tacctcctta cttactgccc gggttaaccc tgacggcaat
    14221 acttgtgctc gattgcttta atcttctcca cccggcaggc atgacggcca ccggcgtagc
    14281 tgccctgtaa aaaggctgcc acaacctcta gggccaggcc tggtccaagc acccgctcac
    14341 ctaaagtcaa gacattggcg tcattatgct cacggctcat gcgcgccgaa aaagtatcat
    14401 gacaatgagc agcacgaatg ccaggcactt tattcgcggc gatactcaca ccaattcctg
    14461 taccacaaca gataatccct aagttatact cgccgccggc tactgcttcc gccacctgca
    14521 gcgcaaaatc aggataatcc actgatgctg ccgacatcgt acctaaatcg tgactgatgt
    14581 agccctcttc agccagataa gtgttgatct gctgctttaa atgaaacccg ccatggtcac
    14641 tagcaatcgc aattttcata ggcaacctcc ttgattaatt cttgactgct taccttcatc
    14701 ctgctttttg acgcctgctg cagctagttt tttcacaatt gtctgcagta ggctctctaa
    14761 ttcctgggct gtagccaaat actcggcggc cgtgccgcca aaagggtctt gtacattgcc
    14821 cgtagagccg ctaaactcgc ggatcgtaga tacttttcct ttcagctgcg gctgctgctg
    14881 caagatgctc tcttgatggg tgtccgtcat tactaaaatt aagtccgcct gagcggccag
    14941 tgctgcggta aaaggctggg actggtgtac tgataaatcg agctgcagcg cgttggccac
    15001 cgttaaggca tgtttgctgg cagacatccc cttgagcgcg gccaagccgg ctgaggcaac
    15061 ccgccagtca cttaaatctg cctgcgctaa ctgtgctgcg gccatcactt gggctaaagg
    15121 actgcggcag gtattacctg tacaaacaaa taacagtaat ttctgctgtt tactaatctt
    15181 tctcacccca cttcaatgac acgaggacca gctgctttct ctaaacggtt catcaatgcc
    15241 aagcccacac ctgccgcgcc atacccttcg gccacaatca cctctacttg gcgttggtca
    15301 aactcccgca gggcggcaaa aagatgactg gccaaatctg ctggctgctg gcgactgcct
    15361 aggacaatta tatcatctcc ctgcagctgt gcagcacttt cttgcgatac taacatccct
    15421 actcttttcc cctgctgctg ccagtcctgc tgcaattgcc gcagtttagc caacagttga
    15481 cccgcattgt gactctttac taaataaagc ggggcctgcg gggcataatg taaatatttt
    15541 aagccgggcg aaggcggtgc ttcgccgctg gtctgcaccc agctagccac agggcagtct
    15601 cgctgcagta ctgtacttat ttgctgcgct gtgatgccgc cagggcgcaa aatgcgtggt
    15661 gagccgccgc ggatgtcaag cactgttgat tcgacgccca ctttactggg gccaccatcc
    15721 aataccccct caatgcgtcc cgccaaatcc tgcaaaacat gggcggcggt agtaggacta
    15781 gggcgtccgg agagattggc actaggacct gccaggggga tagctgcagc ccgcaataaa
    15841 gctaaagctg tgggatgcgc cggcagacga atagccacac tatccagacc accggttgtc
    15901 tctctgggca catggtcttg tttttgtaaa accagcgtta acggcccggg ccaaaagtgt
    15961 ttcgctaaca accaagcttc cgccggaact tctgctgcta aatcactcat ttgtgacgcc
    16021 tcatgaatat gtacaattaa cgggttgtcc attggtcggc ctttggcacg aaagatttta
    16081 gctactactt ggggctgtaa agcattacca cctagaccat agactgtttc tgtggggaaa
    16141 gctaccaacc ctccctggcg cagaatctgc gccgctgctg cggcttccct gcttaaagtg
    16201 gcgggcgtga tgccatcaaa gattagcgtt ccttttatcg tttctctgag cacatgcacc
    16261 cctgctttct ttcaactgct ctgggcgttc aatctgcctt caccatttga caaaggtaga
    16321 gttagatcat accttcaagt ataatatata caataagaga tatctgttcg acagaattag
    16381 caataactcc tttaaaaagc ttcctctttt catatttctt aatggtaatt atttaaaatg
    16441 aaaaccatga ttcagggcac ttaaaaatat cttgcccaaa atgaactttc tcactgccgc
    16501 tattatcatt ttcgccaagt tgtgatagat tgtttttagt gacataaagg agggtactaa
    16561 gagctgatgg ataaaaagca attacatagc ttactaagtc aagtgcaaaa tggcactgtg
    16621 gctgttgctg acgcgctgca gcgcttaaga aacttcgact atgaagattt aggttttgcc
    16681 aaagtggatc atcaccgagc tctgcggcag ggatttccgg aagtaatctt ttgtccccac
    16741 aaaacaacag cacaaattgt cagtatcgcc gcacgtttag cacaaaacaa tactaccgtt
    16801 ttagctactc gtgctgccgc tcacattttt gaggcgctgc aagcgtcgct gccccaggtc
    16861 gaataccatg aattgcccca ggttgtggtc atcaaccgcg accacaatct ccccgcagtg
    16921 ggacaggtcc taatagtttc ggcaggaaca gccgatttag ccgtggccga agaagcggcc
    16981 atcacggccg aactaatggg cagccaggtg aatcgtttat atgatgtggg cgtcgccggc
    17041 attcaccggc tcttagccaa ccaggcacaa ttgcaggctg ccaatgtttt agtggtcgtg
    17101 gccggcatgg aaggtgcttt agctagtgta gttggcgggc tcgtagacaa gcccatcatc
    17161 gccgtaccca cttcagtggg ttatggcgca agctttgggg gagtagccgc actgctgagc
    17221 atgcttaata cctgcgccag cggtgtgtct gtagtcaata ttgataacgg ttttggcggc
    17281 ggctatacgg cagccttaat taatcggctt gccgcccagg aggagagcta actgtgaagc
    17341 ttttatactt tgattgtttt gccggcgcca gtggtgatat gattcttggt gccctgcttg
    17401 ctgcaggagc tactttggat tttgttaaca gcgagttagc taaacttccc ttcaccgggt
    17461 atcagttaaa aacaacgcag gtcatcaaaa agggtattgc tgcgacaagg ctgctggttg
    17521 aaatcacaga agcacagcag ccgcaccgct cttatcatac cattagagaa atgcttaacc
    17581 agagcgatct ggctaaacca gtgcgagatt taagcttggc gatttttgcc cgtttagccg
    17641 cggcggaagg aaaaatccac ggacgttcac cagaggaagt ccattttcat gaagtaggag
    17701 cggtagactc gattattgat attgtgggcg ctgcggcggc tttaactgaa ctagctgcag
    17761 acaaggtcta tgcttcagca ctgcatactg ggcgcggctt tgtgaactgc gcacatggca
    17821 gactgccggt gccggcccca gccactttgg aagtactggc cggggcgcca atctatgcta
    17881 ccgagctgga gggcgaatta ttaacaccaa ccggtgctgc cattctcgcc accattgctg
    17941 aatttacccc cctgccgctg atgactgtac aacaaattgg ctatggggcg gggcaaaaag
    18001 aactaccaat tgccaatgtc ttacgcgtga tggtcggtga aagtgcggac agtgacactg
    18061 ctcagcatga gcaggtgacg gtgctggagg ccaccattga cgatcaaaac ccggagcttt
    18121 atggttatat tatggcaaaa ctttttgcaa tgggggcagt ggatgtcacc atgacaccac
    18181 tatacatgaa aaaaaaccgt cctggcattc tgctgactgt cctagtggca gccgctcagg
    18241 agcaggccgt catcaccacc atcttccaag aaactactac cttagggctg cggcgcagca
    18301 cggcagagaa gttaaggcta accaggcgac agcttacagt ccagactccc tacggcagtg
    18361 tacgcatgaa aattgccgag caagctggcc agatctgtaa tgcggcacca gaatacgccg
    18421 actgcacct
//
LOCUS       DASRMS010000148         8065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_240776, whole genome shotgun sequence.
ACCESSION   DASRMS010000148 DASRMS010000000
VERSION     DASRMS010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 8065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8065
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_240776"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFC74_06610"
     CDS             <1..408
                     /locus_tag="VFC74_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HZK25042.1"
                     /translation="QRVFEYHGAEHKSVACFEAGEELTVVNARKYSTLHPRCGTSFLL
                     LVMTVSIVLFSFFGWPGLWQRLMLRLVLLPFVAGLSYELIQLAGRYRIFRYLTAPGLW
                     LQRLTTREPDDQQLAVALHALHAVLADANKHEV"
     gene            412..1482
                     /gene="prfA"
                     /locus_tag="VFC74_06615"
     CDS             412..1482
                     /gene="prfA"
                     /locus_tag="VFC74_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HZK25043.1"
                     /translation="MIEKLQSIEDHYLEIEKQISDPEVIARQDEWRQLTKEHAHLMEI
                     VNAFRDYKAADTSLQEAKEMLQDSSEEEMNAFLKEEITDHTEKMEELTEKLKVLLLPR
                     DPNDDKSVIVEIRAGTGGEEAALFAGDLLRMYTRYAERLGWKTEIMDANATDIGGFKE
                     VVFAIDGDGAYSRLKFESGVHRVQRVPSTESGGRIHTSAATVAVLAEAEDVEVQINAN
                     DLRIDVFCSSGPGGQSVNTTQSAVRITHQPTGIVVSCQDEKSQLKNKEKAMRVLRARV
                     YEKAQEEQQAELAQARKLQVGSGDRSERIRTYNFPQGRVSDHRIGMTLYKLDQFLDGD
                     LDEMVDGLITTAQAELLKKVDA"
     gene            1479..2330
                     /gene="prmC"
                     /locus_tag="VFC74_06620"
     CDS             1479..2330
                     /gene="prmC"
                     /locus_tag="VFC74_06620"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HZK25044.1"
                     /translation="MSLTVKEALQRASFQLQLAKLAEPRRESEVLLCAVLQQTRAWLY
                     AHGEAEIPPAAADLFGQWLQRRSKGEPYAYLCGEKEFMGLPFTVTPSVLIPRPETEVL
                     VETVLGELKKVKQPHLLELGTGSGAIAVSLAIYLPQATITAVDISAAALAVARSNAER
                     HQVGSRLRFLLGDLYAPVAHEEYTAVVSNPPYVPSADILRLADTVKAYEPRLALDGGT
                     DGLNFYRRLTAELTMLSAAPPLLAMEVGLGQAAAVAELCHSAGYGQVAQIADLAGIPR
                     VILAKLI"
     gene            complement(2538..2954)
                     /gene="nikR"
                     /locus_tag="VFC74_06625"
     CDS             complement(2538..2954)
                     /gene="nikR"
                     /locus_tag="VFC74_06625"
                     /inference="COORDINATES: protein motif:HMM:NF002169.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0010045 - response to nickel cation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HZK25045.1"
                     /translation="MTKLVRFGISMEEQLLKVFDHKIVSQGYVNRSEAIRDLIRNSLV
                     ELAWEDEAQEVAGTITLVYDHHVRGLSDLLNEIQHNCHQLIISTTHIHLDHQNCLEVL
                     VLKGPAGNVREIADKLIGVKGVKHGQLTITSTGQEL"
     gene            3190..3993
                     /gene="sigG"
                     /locus_tag="VFC74_06630"
     CDS             3190..3993
                     /gene="sigG"
                     /locus_tag="VFC74_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HZK25046.1"
                     /translation="MQVRKVNISGVNTYKLPVLSNERMRELFHQLRSGKKAARMELIN
                     GNLRLVLSVLQRFKNRGENLDDLFQVGCIGLMKAIDNFSLEHNVHFSTYAVPMIIGEI
                     KRHLRDNNTVRVSRSLKMLAQKAQHAREELTRKFLREPSIQEIAQVLDVTPEEVVFAY
                     QAINEPVSLHDPVFSDTTDPVYIMDIVSDQKCNADLWVEDITLRQALSILSERERQIL
                     EARFFEGRTQTEIAVEIGISQAQVSRIEKAALRRVRHHMAEEGDSDHVG"
     gene            3983..4600
                     /gene="spoIIR"
                     /locus_tag="VFC74_06635"
     CDS             3983..4600
                     /gene="spoIIR"
                     /locus_tag="VFC74_06635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein R"
                     /protein_id="HZK25047.1"
                     /translation="MWAKRWLVLLLLLPIFLSGAPAPQPTNDALLRLHVRANSDAPAD
                     QAVKYEVRDTVLAVLDEYLQTAGSAADAKVQVASVLPAVLAAAQNTVQAAGFDYAVTA
                     SLGTAQFPTRLYGEQIYRAGSYQALQIYLGRGAGQNWWCVLFPPLCFVEAKTSAPEQE
                     AVVVQAAESAPEDSTPQFKSRLWEWWQKLFGKEQKKLVFAFLKVW"
     gene            4856..6460
                     /locus_tag="VFC74_06640"
     CDS             4856..6460
                     /locus_tag="VFC74_06640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005436 - sodium:phosphate symporter
                     activity [Evidence IEA];
                     GO_process: GO:0044341 - sodium-dependent phosphate
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HZK25048.1"
                     /translation="MVVNIVLQLIGGLGLFLYGMQLMASGMQKAAGDRMRKILELLTA
                     KPIIAVFTGIVATVLVQSSSTTTVMIVGFVNAGLMTLYQAVGIILGANIGTTVTAQML
                     AFDVNALIYPAIGLGSILNFFGRRRTYRYFGQAALGFGVLFLGMSTMSAAMEPARELP
                     FFINMIAAFGVKPLLGVLIGALFTGLIQASGATLALVIAMTLQEIITLPAAMAIILGS
                     NIGTCITAMIACIGTSLSARRTAVAHLLFNVIGVVIALIFFRPFLSLVTATATGITRQ
                     TANAHTIFNVFNTLLFLPFTKQYANLITRLVPGDDVVLEVGPKYLDKRILLTPDVAIG
                     GVRQELLRMATLAREMVVEATQVFANEDTRLIQQVMQKEDLVDSLEKEITLYLAELAQ
                     HSLTKEQSNEVSALMHAVNDLERIGDHAENITRLAEDKVENRLPLSEQAVTQLQEMQA
                     KVDQMLALAIEAFEESDFLLAGQVVDHDDEVDNMERSLRKSHISRISEQICFPPSGII
                     FLDVISNLERIADHTTNFAQVVLGEF"
     gene            6509..7669
                     /locus_tag="VFC74_06645"
     CDS             6509..7669
                     /locus_tag="VFC74_06645"
                     /inference="COORDINATES: protein motif:HMM:NF019522.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HZK25049.1"
                     /translation="MFEKKVRALQLLVLFLSLFLLAGPVNAAKAPEAGEGNETVIMRG
                     RVTAVENMEPAVEVEDYVEIEQLAEIEITSGDHQGETHSLLNALMGHPYFDITLEEGS
                     EVLLWGEVDGDGQLRTVYLQDHLRDRYVYLIAGLFILLLLLVGGRKGIVTIITLGITV
                     LAVFRLLLPLLLRGYAPIPATVLVATGVTLITIIMIGGLHRKTFAAVIGTVGGVLVAG
                     LLALYVGNAASLTGFSSEDAQMLLFMEGGAIDVRGLLFAGIIIGALGAVMDVAMSIAA
                     ASYEVYAVNPAITIREQIKSGMNVGRAVMGTMANTLILAYVGASVPLLLLFMGYDTPY
                     ITIINSDLVATEVVRALAGSIGIIAAVPLTAVSAGLLRKFGKQPKVENTVDS"
     gene            complement(7836..>8065)
                     /gene="larC"
                     /locus_tag="VFC74_06650"
     CDS             complement(7836..>8065)
                     /gene="larC"
                     /locus_tag="VFC74_06650"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nickel insertion protein"
                     /protein_id="HZK25050.1"
                     /translation="RRSTAEKLRLTRRQLTVQTPYGSVRMKIAEQAGQICNAAPEYAD
                     CTSLANQHNVPLKEVYQAAIHAYQSQKLTNK"
BASE COUNT         1975 a         1762 c         2140 g         2188 t
ORIGIN      
        1 caacgcgtct tcgaatatca tggggctgaa cataaatcgg tagcttgttt tgaagccggg
       61 gaagaactaa cagtcgttaa tgcccgtaaa tattctacac tgcatccccg ctgcggcacg
      121 agctttcttt tgctggtgat gaccgtcagt attgtgttat tctccttttt cggctggccg
      181 ggcttatggc aaagactaat gctgcgtctg gtgctcttac cctttgtcgc aggattgtcc
      241 tatgaattaa tccagctggc aggtcggtat cgtatctttc gctatttaac tgcccctggt
      301 ctttggctgc agcggctgac aacacgtgaa ccagatgacc aacaattagc agtagctctg
      361 catgccctgc acgcggtgct ggcggatgca aataagcatg aggtgtaaaa aatgattgaa
      421 aaattacaat ctattgaaga tcattatctg gagatagaaa aacaaatcag tgatcctgaa
      481 gtaattgctc gccaggatga atggcggcag ctgactaaag aacatgctca tttgatggaa
      541 atagttaacg cttttcgcga ctataaggcc gcagatacta gcttgcagga agctaaggaa
      601 atgctgcaag acagcagcga ggaagaaatg aacgctttcc tcaaggaaga aatcactgat
      661 catacagaga aaatggaaga actaacagaa aagttaaaag tgctgctttt accacgtgac
      721 cctaatgatg ataaaagtgt gattgtggaa atacgcgcgg gcacaggcgg tgaagaagca
      781 gctttgtttg ccggtgattt gctgcggatg tacacccgtt acgcagaacg tttagggtgg
      841 aaaacagaaa ttatggatgc caatgccacc gatattggcg ggtttaaaga agttgttttt
      901 gccatcgatg gtgatggtgc ttacagccgt ctgaagtttg aaagcggcgt gcaccgtgta
      961 cagcgtgtac ccagcacaga atctggtggt cgtattcata cctctgctgc cactgttgcc
     1021 gttttagcgg aagcagaaga tgtggaagtg caaatcaacg ccaatgatct acgtattgat
     1081 gtcttttgtt cttctggacc cggcgggcaa agtgttaaca ctactcaatc tgcagtacgc
     1141 attactcacc agcctacggg catcgttgtt tcctgtcagg atgaaaaatc ccagttgaag
     1201 aataaagaaa aagcgatgcg cgtcttacgg gcccgggttt atgaaaaagc acaggaagaa
     1261 caacaggctg aactggcgca agctcgtaaa ttacaggtgg gcagtggcga ccgcagtgaa
     1321 agaattcgca cctacaactt tccccaaggg cgtgtttctg atcatcgcat tggcatgaca
     1381 ctatataagc tagaccaatt tttagacggc gatcttgatg aaatggttga cggcctaatt
     1441 acaactgcac aggctgaatt gctcaagaag gtggacgcat gagtctgaca gttaaagaag
     1501 ccctgcaaag ggcttctttt caactacaac ttgccaagct ggcagaacca cggcgcgagt
     1561 cagaagtttt gctctgtgct gttttacagc agaccagagc ctggctctat gcccatggtg
     1621 aggccgaaat tccacctgca gcagcagact tgtttgggca gtggctgcag cggcggtcaa
     1681 agggggaacc atatgcttac ttatgtggtg aaaaagagtt tatgggactg cccttcaccg
     1741 tcacccccag tgtgctgatt ccccgccctg aaacagaggt cctggtagaa acagtgcttg
     1801 gagaactaaa aaaggttaag cagccacacc tgctggaact tggcaccggc agtggtgcca
     1861 ttgctgtctc cctggcaatc tatttgccac aggccacaat taccgctgtt gatatatccg
     1921 cggccgccct ggctgtagcg aggagcaacg ctgaacgtca tcaggtaggc tcacgcctgc
     1981 gctttttact gggggatctt tatgctcccg tagctcacga ggagtatact gctgtcgtca
     2041 gcaacccgcc ttatgttccc tccgctgata ttttgcgctt ggccgacaca gttaaagcat
     2101 atgaaccacg cctggcactg gacgggggaa cggatggttt aaatttttac cgccgtctca
     2161 ccgcagagtt aaccatgctg tccgcagcac ccccattgct ggccatggaa gtaggactgg
     2221 ggcaagcagc cgccgtagcc gaactttgtc acagtgccgg ctacgggcag gtagcacaaa
     2281 tcgccgatct ggccggcatc ccccgtgtca tcctggcaaa gttgatctag gggtgaactg
     2341 ttatctaagg gcgagaagtt gatgtgggga tgagaagttg atgtgggggc gaaaaattga
     2401 tgtaggggcg aaaaattgat gtaggggcga aaaattgatg taggggcgaa ctgtgttcgc
     2461 ccgcagcccg cagatgtgat gtacggtgtg cctctggcac gtttcctgta taaaaaaaaa
     2521 cgccctgcgt ttttttttta taactcttgg ccggtggaag tgatggtcag ctggccatgc
     2581 ttgacacctt taaccccgat tagcttgtcg gcgatctcgc gtacatttcc tgccgggccc
     2641 ttgagaacta aaacttccag gcagttctgg tggtctaaat gaatatgcgt ggtggaaata
     2701 attaactgat ggcagttgtg ttgtatttca ttaagcagat cacttagtcc gcgtacatgg
     2761 tggtcgtaga ctaaagtaat cgtgccggct acttcctgtg cttcgtcttc ccaagccaac
     2821 tcgactaaac tgttgcgaat taaatcccta atcgcttcag aacggttaac ataaccttgg
     2881 ctgacgattt tatggtcaaa cacttttaat aattgttcct ccatggaaat accgaaacgc
     2941 actagttttg tcacattgat tcctcctcag ggagtggggt ctgcagagcg gcgacgcccc
     3001 gacgctgggt attgatgcaa gagtaaggga atatcaattg tttcggctca aggtgttaaa
     3061 aatcctgccg ctgttttttg gcgtactgtt atggcctaac ggaaaaactt ggttattttt
     3121 ccccccggca tgtttattgg atgaaaatta tggcaatact aacaggcagg aaggttaggg
     3181 gggagtgcca tgcaggtcag gaaagtaaac attagcggcg taaatactta taaattaccc
     3241 gtgctgagca atgaacgcat gcgggagctc tttcatcagc tgcgaagcgg caagaaagcg
     3301 gcgcggatgg agttaatcaa cgggaattta cggttagtct taagtgtctt gcagcgattt
     3361 aaaaatcgcg gtgagaatct agatgattta tttcaagtag gttgtatcgg cttaatgaaa
     3421 gcgattgata atttttctct ggagcataat gtacattttt ctacttatgc cgtacccatg
     3481 attattggtg agattaagcg tcatctacga gataataata cggtgcgggt cagccgctct
     3541 ttaaaaatgt tggcgcagaa ggcgcagcat gctcgggagg agttaacacg gaaattttta
     3601 cgggaacctt caatccaaga aattgcgcaa gtattagatg tgactccgga agaagtagtt
     3661 tttgcttatc aagccatcaa tgaacccgtt tccctgcatg atcctgtttt ttcagatacg
     3721 acagaccctg tctatattat ggatatcgtc agtgaccaaa aatgtaatgc tgatttatgg
     3781 gtggaggata ttactctgcg gcaggcttta tctattcttt ccgaacggga acgtcagatt
     3841 ctagaagcac gcttttttga ggggcgcacg caaacagaaa tagccgtgga aattggtatc
     3901 tcccaagccc aagtctctcg cattgaaaag gcggcgctgc gccgtgtgcg gcatcatatg
     3961 gcggaggaag gggatagtga tcatgtgggc taaaaggtgg cttgttttac tgctgctgct
     4021 accaatattc ttatccggtg cacctgcgcc gcagcctaca aatgatgctc tgttacggct
     4081 gcatgtacgc gcaaacagtg atgccccagc tgatcaagca gtaaaatatg aggtgcgtga
     4141 cactgtgctg gctgttttag atgaatatct gcagacagca ggcagcgcag cagacgccaa
     4201 ggtccaggtg gcctcagtgc tgcctgccgt cttggctgcg gcccaaaaca cagtgcaggc
     4261 cgccggtttt gattatgcag taacggcctc gctaggcaca gcgcagttcc cgacacgttt
     4321 atatggtgag caaatttacc gggccggcag ctaccaagcc ctgcaaatct atttgggcag
     4381 aggtgccgga caaaattggt ggtgcgtctt gtttccccct ttatgttttg tggaagcaaa
     4441 aacttctgca cctgaacaag aggcggtggt ggtacaggcc gccgagtctg cgcctgaaga
     4501 ttctacgccc cagttcaaat cccgcctgtg ggaatggtgg cagaagttat ttggcaagga
     4561 gcaaaaaaaa ttagtttttg ccttccttaa agtgtggtag gccagggaac ttgtcggtgt
     4621 ggtcgctagg ttagtaaagt ggtgtaaaca gtaatcaggg cagcttgtct cggctgcggc
     4681 agcaggcagg cggcgtccgc tgtggtgtaa agctgcatca aacagacaaa atgaagaagt
     4741 taatgtttat tgaaaaagtg acatccttcc gctataataa aaatagtatg tccatctact
     4801 taacaagaca acaaattcgg tgctaacaaa aaacgccgtt attcggggag gatgcatggt
     4861 cgttaatatt gttctgcagc taattggggg gctggggctt ttcctgtatg gcatgcagct
     4921 aatggcttcg gggatgcaaa aagcagcagg ggatcgcatg cgtaaaattc tggagcttct
     4981 gactgccaag ccaattattg ctgtctttac gggcattgtg gctacagtgc tggtgcaaag
     5041 cagcagtacc accacagtta tgatcgtcgg ttttgtcaac gcaggtttga tgactcttta
     5101 tcaggccgtt ggtattattc tcggtgccaa tatcggcact acagtgacgg cacaaatgct
     5161 tgctttcgat gtgaacgcct taatttatcc ggcgattggt ctgggttcca tcttaaactt
     5221 ctttggccgc cgtcgtactt atcgctattt cggtcaggct gccttaggtt ttggtgtgct
     5281 ctttctgggg atgagcacca tgtcagctgc tatggaaccg gcacgtgagc tgcccttttt
     5341 cattaacatg attgctgctt ttggggttaa gcccttactt ggtgtactaa ttggggcatt
     5401 gtttacgggg ctcattcaag ctagtggtgc tactctagct ctcgtgatag ccatgacgct
     5461 gcaagagatt attaccctgc ctgcagcgat ggccattatt ctcggctcta atattggtac
     5521 ttgtatcacg gcgatgattg cttgtattgg cacttccctg tcggcacgac gcaccgcagt
     5581 agcgcactta ctttttaatg tcatcggtgt ggtgatcgct ttaattttct tccggccttt
     5641 tcttagttta gtcacggcga ctgctaccgg gattactcgc cagaccgcca atgcgcatac
     5701 aatctttaat gtttttaata ctttactttt cttacccttt actaaacagt atgccaatct
     5761 cattacgcgc ctggtaccgg gtgatgacgt ggtgctggag gttggcccca agtatttgga
     5821 taaacgtatt cttttgacac ccgatgtggc catcggcggc gtacggcaag aattattgcg
     5881 catggcaact ttagcccgtg aaatggtagt tgaggctacg caggtgtttg ccaatgagga
     5941 taccagactg attcagcagg ttatgcaaaa ggaagatcta gttgatagtc tggaaaaaga
     6001 aataactctt tatctggcgg agctggcaca acactcccta accaaagaac aatctaacga
     6061 agtatcagcc ctgatgcatg cagttaacga tttagaaaga attggcgatc atgccgaaaa
     6121 tattactcgt ttggctgaag ataaagtgga aaaccgccta ccgctttctg aacaagctgt
     6181 gacgcagctg caggagatgc aggcgaaagt tgatcagatg ctggccttag cgattgaggc
     6241 ttttgaagaa agtgacttct tgttagcagg tcaagtagta gaccatgatg atgaagttga
     6301 caatatggaa cgtagtttac gcaaatcaca cattagccgt attagtgaac aaatctgttt
     6361 cccgcccagt ggcatcatct tcctggacgt aattagtaac ctcgagcgca ttgccgatca
     6421 cacgaccaat tttgcccagg tagtattagg tgaattttaa ggtatgtggt aaggatagtg
     6481 cgtaaattga gaagatggag gaaagtagat gtttgaaaaa aaagtgaggg ccttgcaact
     6541 gctggtcctt tttttatccc tgttcttgct ggctggaccg gttaacgccg ctaaggcccc
     6601 tgaagctggt gaaggtaatg agacggtgat catgcgcggt cgcgtcactg ctgtcgaaaa
     6661 tatggaacca gctgttgaag tagaagatta tgtggaaatt gaacagctgg cggaaataga
     6721 aatcacttcc ggcgaccatc aaggtgagac tcattctttg ctgaatgcct tgatggggca
     6781 tccctatttt gatattaccc tggaagaagg cagtgaagta ttgttatggg gtgaagttga
     6841 tggggacggg cagctgcgta cagtgtattt acaagatcat ctgcgagatc gctatgttta
     6901 tttaatagct ggtcttttta ttctgctcct gctgctagtt ggtggccgca aggggatcgt
     6961 aaccattatt acactgggta taacggtgtt ggcagtcttt cggctcttgc tgccgctgct
     7021 gttgaggggg tatgccccca ttccggcgac ggtgctggta gcaaccggtg ttacattaat
     7081 taccattatc atgattggcg gcctgcatag aaaaactttt gcggcggtta ttggtactgt
     7141 tggtggtgta ctggtggcag gccttttggc tctctatgtt ggtaatgccg cgagtctcac
     7201 gggcttctct agtgaagatg cacagatgct gctgtttatg gaaggcggcg ccattgatgt
     7261 ccgcgggctg ctctttgccg ggattattat tggggcctta ggtgccgtga tggacgtggc
     7321 catgtccatt gccgccgctt cctatgaagt ttatgcggtt aaccctgcca tcacgattcg
     7381 ggagcaaatt aagtctggta tgaatgtggg acgtgctgtg atgggcacca tggctaatac
     7441 cttgattttg gcctatgtcg gtgcctcagt acccttgctt cttttgttta tgggttacga
     7501 tacaccttat attacaatta tcaactcaga cttagtggca acagaagtag tacgagcctt
     7561 ggcgggcagt attggtatta tcgctgccgt accgctcacc gctgtttctg ctggtctgct
     7621 gcgtaagttt gggaaacaac ccaaggtaga aaatactgtg gattcgtaaa tcatttagtg
     7681 tacacatcaa gagggcgcct gggcgccctc ttttctttta gcggtgcggg cgcacacaga
     7741 ggcgccctta cggtgagagt gcagcccaac ggtcaggatg catgccccac attgtggaca
     7801 tcgctatgtt ttgccgttcg ttttgtctta caggattatt tgttggtcag cttttgcgat
     7861 tgataggcgt ggatggccgc ttgatagact tcttttagcg gtacattgtg ttggtttgct
     7921 aaagaggtgc agtcggcgta ttctggtgcc gcattacaga tctggccagc ttgctcggca
     7981 attttcatgc gtacactgcc gtagggagtc tggactgtaa gctgtcgcct ggttagcctt
     8041 aacttctctg ccgtgctgcg ccgca
//
LOCUS       DASRMS010000149         2007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_442237, whole genome shotgun sequence.
ACCESSION   DASRMS010000149 DASRMS010000000
VERSION     DASRMS010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2007
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_442237"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            224..730
                     /locus_tag="VFC74_06655"
     CDS             224..730
                     /locus_tag="VFC74_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3D domain-containing protein"
                     /protein_id="HZK25051.1"
                     /translation="MSKKGELLQAIAVLALSLAVLVAAKSTASLKVEVKELQLRQEVL
                     EVELQRLREEGIKVDIKIEDIFPDAKETVMEVTAYAPLDPSAVEGLCYSGNPNITANG
                     ERPVPGETVAASLPFGTVLYIEGLGLRRVNDRGVGAGRVDVVTETRAQAFKLGRSMRR
                     VLILERGR"
     gene            732..1175
                     /locus_tag="VFC74_06660"
     CDS             732..1175
                     /locus_tag="VFC74_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25052.1"
                     /translation="MEKKIPRSDYRFVERLLYQQKTHDTVIAELKAELDEMIPSSTSS
                     YVTFDHNKKNPELTQPERIADKRLYSVRGKYLQEEIARRKRHQAAITEAMNALNDTES
                     QLVWLYYHLEKTPRDCWRTMGYEKSRWYEIKNETVCKVARFLGLA"
     gene            1188..1451
                     /locus_tag="VFC74_06665"
     CDS             1188..1451
                     /locus_tag="VFC74_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25053.1"
                     /translation="MEGYKNLAAAVVKAAVDDLDAAYRKIIKWEEEGKQRKIKLTQDQ
                     QTAVNFFKDGSEMREFYFSMLGIDGLPHEIISQCDYVEKACKI"
     gene            1629..1970
                     /locus_tag="VFC74_06670"
     CDS             1629..1970
                     /locus_tag="VFC74_06670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012720777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HZK25054.1"
                     /translation="MVMVLNKKLLGYIKQGWYVKFYQSKEWRATRARVLQRDRYECQT
                     CKDRGKVTVGTEDRPLEVHHIKHLKDYPLLGLAEDNLLTLCASCHNEEHPEKLIKAQP
                     KPKFENEERWE"
BASE COUNT          605 a          354 c          596 g          452 t
ORIGIN      
        1 tgggcgtgtt cgtggggata tttgcaggct tttttatcgt cggtatagct gtaggggtcg
       61 ggcagtttat ctggtttgga ggtggcgggg atgagtaacg agcaggaagt cttgtactgg
      121 tgtgtcttct gcagttataa agagaggtca gcgcccacgg aggattttaa gttttgtccc
      181 cgctgcggaa atggaatgtt tatttggggg gaggtcaatc acaatgagca aaaagggtga
      241 gttgttgcaa gctatagctg ttttagcctt gtccttagcg gtgttagtag cggcaaaatc
      301 tacagctagt ttaaaagtcg aagttaagga gttgcagctt aggcaagaag ttttagaggt
      361 tgaattacaa agacttaggg aagaaggcat caaggtcgac atcaagattg aggacatctt
      421 ccccgacgct aaggaaacgg ttatggaagt cacagcttat gcgccgcttg acccgtcagc
      481 agtggaaggc ttgtgttata gtggcaatcc taacatcact gcaaacggtg aacgaccggt
      541 accgggtgaa acagtagcag caagcctgcc ctttggcacg gtgctgtata ttgaggggct
      601 gggcttgaga agagttaatg acaggggagt aggtgcaggc agggttgatg tggtgacaga
      661 gactagagcg caagcgttta agctgggcag gagcatgcgg cgggtactga tactagagcg
      721 ggggagataa cttggagaag aagataccgc gcagcgatta caggtttgta gagaggctgc
      781 tttatcaaca aaaaacacac gacaccgtaa ttgctgaact gaaagctgaa cttgacgaaa
      841 tgattccgtc gtcaacttct tcctatgtaa ccttcgacca caacaaaaaa aatccagagt
      901 taacacaacc agagcggata gcggacaagc ggctgtacag cgtaaggggt aagtatctgc
      961 aagaagaaat agcgcgaagg aagcgacacc aggcggcaat tactgaagcc atgaacgcac
     1021 tcaacgatac agagagccag ttagtgtggc tgtactatca cttagaaaaa acgccgcgcg
     1081 actgctggcg cacaatgggg tatgaaaaaa gccggtggta tgagattaaa aatgagacag
     1141 tttgcaaagt tgcgcgattc ttggggttag cgtgaaagga gaatgttatg gaagggtata
     1201 aaaatctagc ggccgcggtg gttaaggcgg cggttgatga cctagatgca gcatacagga
     1261 aaattataaa atgggaagaa gaagggaagc agaggaagat aaaactaaca caggatcaac
     1321 aaacagcagt caattttttt aaggatggaa gcgaaatgcg cgagttttac ttctcgatgt
     1381 tgggaattga tggactgcct catgagatta ttagccagtg tgattatgtt gaaaaggcat
     1441 gtaaaatata aagaccggac aaatagcgga cggattcgcc ttgaaaggct aagtatagtt
     1501 gatgtatgat taaggtggaa taactaactg aatacataaa ccgtcctttg gggcggttat
     1561 ttttattggt gtgcctacgg gcggggagta cacatccata gagtgccagg gtgggagccg
     1621 atgtgtaaat ggtgatggtg ttgaataaaa aattgttagg ctatatcaag caggggtggt
     1681 atgtaaagtt ttaccagtcc aaagagtgga gagcaaccag ggcaagagtg ctgcagcgtg
     1741 accggtacga gtgccagacg tgtaaggata ggggcaaggt gacagtaggc acagaggaca
     1801 ggccattaga ggttcaccac atcaagcatc taaaggacta tccattgtta ggactggcag
     1861 aagataacct tttgacactg tgcgcatcct gccacaacga agagcatccg gagaaattga
     1921 ttaaagcgca accgaagccg aagtttgaga acgaggagcg gtgggaataa gctccccccc
     1981 tatcaaaaaa acggattccc ctaggga
//
LOCUS       DASRMS010000150         5106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_776225, whole genome shotgun sequence.
ACCESSION   DASRMS010000150 DASRMS010000000
VERSION     DASRMS010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776225"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            97..495
                     /locus_tag="VFC74_06675"
     CDS             97..495
                     /locus_tag="VFC74_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK25055.1"
                     /translation="MDALGKTTVSEDVFQSIAEIALKDIEDIVTKEKKGPFAGISRIF
                     AERFSPQVTVKRDENAEGEFGQVGYELRLAVMYGVKIPEAALKVRSSVIKTVENLTGY
                     KVTKVDIVVDRIVEIKELEADIDQAKETRE"
     gene            complement(724..1011)
                     /locus_tag="VFC74_06680"
     CDS             complement(724..1011)
                     /locus_tag="VFC74_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HZK25056.1"
                     /translation="MKYRIQVYQKNTIESDAWFSIHALDYHPELLERLTLLGIITVKD
                     EMIHSSDLARLRKLFRLRSCLGVNVTGAAIILDLLARVEELQEEIRHLRGY"
     gene            complement(1014..2009)
                     /locus_tag="VFC74_06685"
     CDS             complement(1014..2009)
                     /locus_tag="VFC74_06685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HZK25057.1"
                     /translation="MAVKFQDYYEVLGIKRTATEKEVKTAYRKLARKHHPDLHPGENQ
                     AAAEEKFKQINEAYEVLIDADKRAKYDRLGENWQAGENFQPRPDMDGMHFYSTSGDTG
                     GFSDFFETLFGGGFNNFQGNQGFRQRHTNMRGEDVEAELTLTLEEALRGGEKTVQLAL
                     PSACEQCGGSGISGSGFCPACAGRGQVQTTSTLTVKIPPSTREGTKIRLRGQGGGRSG
                     QRGDLYLRVRLLAHPVFQVQEHDLEAALVVMPWQAVLGDKVSAPTLEGTVSVTLPPAT
                     HAGKRLRLRGKGLPKKDNSRGDLYLRVVIDIPTQVSEKERELYHGLAAERSKEES"
     gene            complement(2034..3893)
                     /gene="dnaK"
                     /locus_tag="VFC74_06690"
     CDS             complement(2034..3893)
                     /gene="dnaK"
                     /locus_tag="VFC74_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HZK25058.1"
                     /translation="MAKTIGIDLGTTNSVVAAMEGGEPVVITNAEGARTTPSVVSYKG
                     NERLVGQIAKRQGALNPERTLTSVKRFIGRNYSEVTEEITLVPYKVVAGPNDAVRFEI
                     DGTLYSPEEISAQVLKKLVVDASSYLGETVRDVVITVPAYFNDAQRQATKDAAVIAGI
                     NVLRIINEPTAAALAYGLGKDINETILVFDLGGGTFDVSILDVGDGVFEVRSTNGDTH
                     LGGDNFDREIVNWLAQEFKKDHGFDLRNDKQALQRLTEAAERAKTELSTTMETQINLP
                     FITADANGPQHLDIKLTRAKFNELTKELVERCREPIKDALRDAKMTADNIDQVILVGG
                     SSRIPAVAELVKELAGGREPNRSVNPDEVVAIGAAIQGGVLTGDVKDVVLLDVTPLSL
                     GLETMGGVMTRLIERNTTIPTRRAEVFSTAEDNQPAVDIHILQGEREMAKDNRLLGQF
                     KLDGVAPAPRGTPQIEVTFDIDANGILSVTAKDKGTSKEQHITISGSTNLDQGEIERM
                     VENAKRYAEEDKKLKELAEARNEADAMVYTSQRQMEELGNKAPQSEKSKVTDLITELT
                     QMLDDHADAQKIRTLTQQLQQQVTALLAASTKEAPSPADHGNDDVVDAEFEEQ"
     gene            complement(4354..4704)
                     /locus_tag="VFC74_06695"
     CDS             complement(4354..4704)
                     /locus_tag="VFC74_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25059.1"
                     /translation="MWLIEYNVKAPYEFLEVGKLRRFFFYSLIVHLSIVVVSVALQRT
                     EVIARGSIAVFVGTVLIAGSIVRGARGWEFLGTTQEEQKEMFAYRNNQGLKALAFLIP
                     NLITIGLYAYLIGF"
BASE COUNT         1413 a         1243 c         1028 g         1422 t
ORIGIN      
        1 atattaacag ataataaaca atactaatgt ttaaaggtca tggccaatat ttatcgaaat
       61 aagttatatc agcaataaaa ggaggtagat atatttatgg atgctttagg taagaccacc
      121 gttagtgaag atgtattcca aagtatagca gaaattgctc tcaaggatat tgaggatatt
      181 gtaacgaaag aaaaaaaagg tccgtttgct ggaatatcaa gaatatttgc agaaagattt
      241 tctccccaag ttacggttaa aagagatgag aacgctgagg gagaatttgg ccaagtaggg
      301 tacgaactta ggttagccgt tatgtacggg gtaaaaatcc ccgaagctgc gctcaaagta
      361 agaagcagtg tcatcaaaac tgtagaaaat cttactggct acaaggttac taaagtcgac
      421 attgtcgttg atcgaatcgt tgaaataaaa gaactagaag cagatataga tcaagcaaaa
      481 gaaactagag agtaaagtca aactcacaaa gaaaaaaact accctaccag cctggtaagg
      541 tagttttttt cttctaccgc gccgaaagat ggggatttgc ttgtttaatt gccagacaat
      601 atcacaaata aaggaataaa atactccctc gtggcttaga tggcaagtat gaagataatc
      661 tggttatcaa tgatgataat ctaaattccc cgcaagagag tttacgggga atttattgta
      721 cttttaatat cctcgtaagt gtcggatttc ttcctgcaat tcttctaccc gagccaacaa
      781 atcaaggata atggcagctc ctgtaacatt aacacctagg catgaacgca ggcggaaaag
      841 cttgcggagt cgcgcaaggt cgctgctgtg gatcatttca tctttaaccg taataatgcc
      901 aagtagagta agccgttcca ataactctgg gtgataatca agagcatgta tactaaacca
      961 agcgtcactc tctatcgtat ttttctgata gacctgaatg cgatacttca tggttaggat
     1021 tcctccttag agcgttctgc ggctaaacca tgatatagct ctcgttcttt ttcactcacc
     1081 tgcgtgggaa tatcaataac cactcgcaaa tagagatctc ctctgctgtt atctttttta
     1141 ggcagtcctt ttccacgtaa ccgcagccgt ttacctgcat gtgtagctgg aggcaaagtt
     1201 acagaaacag tcccctccaa tgtgggtgca ctgactttat cacccaaaac agcttgccag
     1261 ggcatcacca ctaatgctgc ctccaaatca tgctcttgta cttggaaaac aggatgggca
     1321 agtaatcgca cacgtaagta aaggtcgcca cgctgtccag agcgaccacc gccctgaccg
     1381 cgtaagcgga tcttcgttcc ttcccgggta gacggaggta tctttaccgt taaggtacta
     1441 gtagtctgga cctgaccccg accagcacag gctggacaaa atccactgcc actgataccg
     1501 ctgccaccac actgctcaca ggcactaggc aaagccagct gcactgtctt ctcgccaccc
     1561 cgcaaagctt cttctaaagt gagcgttaat tcagcttcca catcttcacc acgcatgttt
     1621 gtgtgacgtt ggcgaaaacc ctgattgccc tgaaaattat taaaccctcc accaaaaagc
     1681 gtttcgaaaa agtcactgaa gcctccagta tcgccgcttg tagagtaaaa gtgcatgcca
     1741 tccatatcag gtcgtggctg aaaattctcc cccgcctgcc agttttcacc cagtctatca
     1801 tacttggctc gtttatcggc gtcgattaaa acttcatagg cttcgttaat ttgcttaaac
     1861 ttttcttccg ccgccgcttg attttcccca gggtgtaagt cgggatgatg tttacgggcc
     1921 agtttacggt aagccgtttt tacttccttt tccgtggctg tacgttttat accgaggact
     1981 tcataataat cttgaaattt tacagccatc gcttatcccg ccatttctct aatttattgt
     2041 tcttcaaatt cggcatccac aacatcatcg ttaccatgat ctgctggaga aggagcttct
     2101 tttgtagacg cagcaagtaa cgctgtcact tgctgctgta gctgctgagt aagggtacgt
     2161 attttttgcg catccgcatg atcatctagc atttgtgtta gctcggttat caggtcagtc
     2221 actttgcttt tttcgctttg tggtgcttta ttccctagct cctccatttg ccgttgcgag
     2281 gtatagacca ttgcatctgc ttcgttgcgt gcttcggcta gctctttgag ttttttgtct
     2341 tcctctgcgt agcgcttggc attttccacc atgcgctcga tctcaccttg gtctaggtta
     2401 gtggagccac taatggttat atgctgttct ttactcgtcc ccttgtcctt cgctgttaca
     2461 ctgaggatgc cattggcatc aatatcaaac gtaacttcga tttgtggtgt gccacggggc
     2521 gctggtgcaa caccatctag tttaaattgt cctagcaatc tattatcctt agccatttcc
     2581 cgttcaccct gcaaaatatg aatatcaact gctggctgat tatcttcggc agtggaaaaa
     2641 acctcggcac gtcgggttgg aatggtggta ttacgttcaa ttaagcgtgt cattacaccc
     2701 cccatggttt ctaaccctag ggaaagaggc gttacatcca acaacacaac atcttttacg
     2761 tcccctgtta ggacaccgcc ctgaatcgca gctcctatgg ccactacttc atctgggttt
     2821 acactgcggt tcggttcgcg tccccctgcc agttccttca ctaattccgc cacagcagga
     2881 atacgggaag aaccacccac taaaataact tgatctatat tatctgcagt catctttgca
     2941 tcacgtaaag catctttaat gggctcacga catcgttcca ctaactcttt tgtaagctca
     3001 ttaaatttcg ctctcgtcaa cttaatgtct aaatgttgag gtccattggc gtcagctgta
     3061 ataaagggta agttaatctg cgtctccatc gtggtggaca gctcagtctt tgctctttct
     3121 gccgcttcag ttaatcgctg caaggcttgc ttatcatttc gcaaatcaaa gccatgatcc
     3181 tttttaaact cttgtgctaa ccaattcaca atttccctat caaaattgtc gccacctaaa
     3241 tgtgtatcgc cgttggtgga acgtacttcg aaaacaccat ctcccacatc aaggattgag
     3301 acatcaaaag taccaccacc taaatcaaaa acaagaattg tttcattgat gtctttcccc
     3361 aaaccgtaag ctaaagcagc cgctgtgggc tcattaatga ttcgtaacac atttatacct
     3421 gcgattacag cagcatcttt agttgcctgt cgctgcgcat cgttaaaata agctgggaca
     3481 gtgatcacaa catcccttac cgtttctcca agatatgaag aagcatcgac cactagtttt
     3541 ttgagaactt gtgcggaaat ttcctctggt gagtataaag taccgtcaat ctcaaagcga
     3601 actgcatcat taggtcctgc taccacttta taggggacaa gagtaatttc ttctgtgacc
     3661 tcagaataat tgcgtccaat aaagcgtttc acggaagtta acgttctttc cggatttaag
     3721 gcaccctgac gttttgcaat ttggcccacc aaacgctcgt ttcctttata tgatacgaca
     3781 gatggagttg ttcgtgcccc ttccgcattg gtgattacga cgggctctcc gccttccatt
     3841 gctgccacca cagagttggt cgtgcctaaa tctataccta ttgtctttgc catctgtaca
     3901 cctccaaatt atatgatgtt catactgaca attataccca ataccggctg tgcagtactt
     3961 atcaaattat tacaaattgt cgttagtaga gatactgaca cgctccagat aaattgttgc
     4021 ccccctgcga caggggacgg ttctcctgtc gcacaacttt gcggcacctc aggcgctaca
     4081 atgcatatgg ttttgtagca aacacgaggg acgtccctgg aagtgtacag gtgcggcaca
     4141 taaacaaaca acgcaccaac ataagttaag tgcgttgcag aataatagtc tggcaaagaa
     4201 ctcccggttt aacctagtga gcaaatctgt cttagctgcg caccaggggg gactctgcta
     4261 aaatacagcg tgagaaagaa ccgtccccta acacgtatac ttgcacataa attgttgacc
     4321 ccatgcgaca ggaaaaccgt cccctgtcgc atgttaaaaa ccaataagat atgcatacaa
     4381 gccaatagta atcaggtttg gaattaaaaa cgcaagagcc ttcaaacctt ggttatttct
     4441 gtatgcgaac atctccttct gctcttcttg tgtagtacct aaaaactccc acccacgcgc
     4501 accacgcaca atggatcctg caattagtac cgtgccaaca aaaacagcta tactaccccg
     4561 tgcaataact tcagtcctct gtagtgcaac gctcaccacc acaatgctca aatgcacaat
     4621 caaactatag aaaaaaaacc gcctcaactt acccacctcc aaaaactcat aaggtgcttt
     4681 tacattgtat tcaataagcc acaacaatta tcctctgcaa ttttttttgc cccgagtaag
     4741 attcgcaacc tcggcctaac ccagcgaaca gagtgagcgg agttatcagg atacaagtaa
     4801 cgctaaagag tttacaattt actgcctcat caaacagtgt ggtcacctgg ttatgtagca
     4861 ttcgcccaag gaggggacat gtccctggga gaatcacagg tgcgggatat aaaaataacg
     4921 acgtcaccga ttactcagtg cgtcgttata aagaggttct ggcagagacc tactctcggc
     4981 ctaacctagt gagcaaagcg aacggatgat actaagtcag cgtatcgcta aagaggagtt
     5041 aagtatcttt taactttata ccaagcacgg gttacctgat tatgtagcat tcgccgcagg
     5101 gacacg
//
LOCUS       DASRMS010000151         1216 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_444044, whole genome shotgun sequence.
ACCESSION   DASRMS010000151 DASRMS010000000
VERSION     DASRMS010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444044"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(101..880)
                     /locus_tag="VFC74_06700"
     CDS             complement(101..880)
                     /locus_tag="VFC74_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25060.1"
                     /translation="MESHKMKVNKALLFFCEAHFLPFPPQSNSRVFLWVVIMKPRKTV
                     FGVGVNDANYAVVKREELGCVGGKRKRKLVWGCAYYSRWRDMLRRCYDAKYQECKPTY
                     AGCTVSKDWLTFTNFKNWMESQDWEGKHLDKDLLFEGNKIYSPETCVFVSPMVNTFTI
                     DRGNDRGKWLIGACWDKQTEKFLAQCSNPFTNKNEKLGLFTCEQEAHEAWLKRKLELA
                     HELAAIQTDERIAKALIDRYSNPYIDKAIGLNKPTVGEALK"
     gene            complement(947..>1216)
                     /locus_tag="VFC74_06705"
     CDS             complement(947..>1216)
                     /locus_tag="VFC74_06705"
                     /inference="COORDINATES: protein motif:HMM:NF023181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HZK25061.1"
                     /translation="DLFPNQQVIDVIQSALTEEEFAGYCKGNALKYRLRAGDKGDALK
                     CIAKANWYQNKLREIKETNAETEWPDESRIDAIGQNGNDGVVYSE"
BASE COUNT          282 a          304 c          231 g          399 t
ORIGIN      
        1 atttctgagc actcatgcac tgttttgctt cgccataatt cctttagatg tgcaatctct
       61 ttagcgctcc attctttgcc tctgtgtttt tttgtcgagg tcattttagt gcctcgccca
      121 cagtcggttt atttagacca atagccttat caatatacgg attcgagtaa cgatcaatca
      181 gtgccttggc aattctttcg tctgtttgga ttgcagctag ttcgtgagct agctctagtt
      241 tacgtttcag ccacgcttca tgagcttctt gttcgcaggt gaataggcca agtttttcgt
      301 ttttatttgt aaatggattg ctgcactgag ctagaaactt ttctgtttgc ttatcccaac
      361 atgccccgat taaccactta cctctgtcat tacccctatc aatggtgaat gtgttgacca
      421 tgggggagac aaaaacgcac gtctcagggc tatatatttt gtttccctcg aataacaggt
      481 ccttatcgag atgttttcct tcccaatctt gactctccat ccagttttta aagttggtaa
      541 acgtaagcca gtctttagac acagtgcatc cagcataagt tggcttgcac tcttggtatt
      601 tggcatcgta gcacctccta agcatatctc tccatctgct ataataagca cagccccata
      661 ccagtttacg ctttcgtttg ccgcctacgc agccaagctc ctctcgcttt acaacagcat
      721 aattagcgtc gttgacacca actccaaata ccgtttttct aggtttcata atcaccaccc
      781 ataaaaaaac tctgctgttt gactgtggtg gaaaaggcag aaagtgcgcc tcacagaaaa
      841 acagcagagc tttgttaact ttcattttat ggctttccac ggcctagact ctaattatac
      901 acactaccgc gagtaatgcc agccttttta agctggcaaa tgctgtttat tcagaataaa
      961 ccacgccatc atttccattc tgcccgatcg cgtcaatgcg tgattcgtcc ggccattcgg
     1021 tttctgcgtt tgtttcttta atttccctta gcttgttttg ataccaattt gctttggcga
     1081 tacattttag agcgtcaccc ttgtcaccgg cgcgaagtcg atacttaagc gcattgcctt
     1141 tgcagtatcc ggcgaactct tcttctgtca gagccgattg gataacgtcg ataacctgct
     1201 gattgggaaa cagatc
//
LOCUS       DASRMS010000152         6157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_777744, whole genome shotgun sequence.
ACCESSION   DASRMS010000152 DASRMS010000000
VERSION     DASRMS010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 6157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6157
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777744"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..1614)
                     /locus_tag="VFC74_06710"
     CDS             complement(208..1614)
                     /locus_tag="VFC74_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HZK25062.1"
                     /translation="MFNSAALLTLATLAGLILGFAREWLLIDAWGAGKQSDAFLIALF
                     LPEALRMTLAAGILSAAALPLYQQRTAGEQPQWLNSFAACLLLAGVILTGMLSVLAPG
                     WINLIGPGLEPSAKLAAVADFKLLIWCVPLFFLHAVFTVILQAQQRYILAGLGSFLFN
                     LFPVGWLLIARSEVTTQGLSLAFILGSFAMLSVLIPSAITLGWRPWLMRGALRVGAEL
                     WGKLGPLLLSNAASQGLTLLERAVASTLGEGMVTWVNLARKLINLPLIALMALNQVLL
                     SILSSRLLEQRLLILRQGLAVTSCIALAAAVGLVAISPSLSELLFRQLENISLLALLL
                     AWFAVPLVFGAWNPLLARLAYADGNTQLPLRCELTGNAVNALMLLALPLFMGVIGIAL
                     AAVCGALITAFLLLKQQNLLHDVPWKLQLILSSMALSSAAYIIYPMPPGIVQITWSIV
                     AGALALIIAAILYKPWKA"
     gene            complement(1621..2433)
                     /locus_tag="VFC74_06715"
     CDS             complement(1621..2433)
                     /locus_tag="VFC74_06715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HZK25063.1"
                     /translation="MRHRVYLDFDVYEKLPEYAGFLNVSLEELQSTVEYMLQHNILFE
                     ENEKILSISSIDIEDVIENDIAKRFYAALKKTIPSNFFPNYGKNWATVKDRCLRIWEQ
                     VYNIIINKIPSHTVRIAWLRLGGAKIGKGSTIWRHTEVLGVENLVLGEDSTIAWHCQV
                     DARAGLVIGDHVAIASHVLIVAGSHDLTAPEFWSVGAPIYIEDYVWVASRALIAHGAR
                     LRRGCVVTANTVVAKEIPAYKIIGGTGAKAMGERPHNLNYKVGGKGLFTLLH"
     gene            complement(2449..3822)
                     /locus_tag="VFC74_06720"
     CDS             complement(2449..3822)
                     /locus_tag="VFC74_06720"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HZK25064.1"
                     /translation="MTIERILAVLLGLSFGLAALVLTPAKVLVLMAALLVFLIVMRKP
                     LYGLILFAVLATSIPYTTIEIGVRTTISEAVLAVTWAGVFWHALIGLMRQRLSWQSAE
                     LSMLHLFLYSLIPLIYGVLMVSAEGSSIVNWLRWMLNLSVLFLVPLLLRTDADRDKVI
                     VAFLSGSLLMLLVSIGYFLKDRDATTFIPLLETLRYSHPEAVIDIFSANYQRMASPWV
                     HPNLTGGILALTVPLSLLYGWTRPGWRRFLGLSVAILGCAGLLFSISRGAILALLLVL
                     FWLSYKRLPYSARIIALGAVLAVLLVMFYPPLQERLLSMFSASNASTGIRFDEYRMFP
                     QAMATYPVGIGFKVDPPVPGSGLLGISNLWLNVIYKIGLPGLLLFSILIWRWWREVKP
                     RNNITKITYENAIWVGSLSGVLAALTTGLFDHYYSFTFVIIALFWLLVGVSLQQVRLF
                     PSKTSHA"
     gene            complement(3824..4915)
                     /locus_tag="VFC74_06725"
     CDS             complement(3824..4915)
                     /locus_tag="VFC74_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HZK25065.1"
                     /translation="MHIGIDYRPVSVAPFSGIGRQVKGLEAVLQGRDNTQLSLLAVVP
                     EDHPLRAKVTSPEWTTPLAGLQQPHKRLRFEAGFIPRALADNKIDLYIATANMGLPIG
                     RKNKNTRLALLLHDLFQLTEDNHHTSKLKASVYRVLDRLSIAWSVYAADQIWCPSKFT
                     AEQVKNKFPAASKKVRVLNNLVSEFTADTEPLEQLPQNYWLIVGTREPRKNIKLFIQV
                     WMMLRSQMSVPDLVLVGSSDDCPEYRHATGIHWLSGITEGQLHTAYRNAASLWQPSYA
                     EGFGLPVIEALSVGTPVVTALGSALDEITPEFSPRFDPYSFADLEKCMREVGAHPLLK
                     NQIHYTQWSAQYTYAAYQKRVNQLILELS"
     gene            complement(4918..6123)
                     /locus_tag="VFC74_06730"
     CDS             complement(4918..6123)
                     /locus_tag="VFC74_06730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK25066.1"
                     /translation="MVKPMRILWILPYLPWPATSGGKTRQLHLLRMLAAADFKITLLV
                     QSKKPANEATHVALAPYLERLIVLPRRSMKHPMTLLRIFFGKWPILTCVNGFSKPLQA
                     VFSELLSEQWDVIQIEHSYSFQPYDQLLKARQQSFILTEHNLESSLGGATYGRFPKIF
                     KPFVVFDQWRARTWEKYVFARGQRILAVTETDADNIQRLTATPVDVVVNGVDTASFSL
                     VEPDFNALSVLFVGNYEYPPNGDAIGWALTEIMPLVWQTVPSARFIVAGYALPDEWAK
                     RWPDQRIDWKGFVEDLPELQSQCSVFLAPLRHGGGSKLKVLEALAAGLPLISTAQGVS
                     GLSIEAEDAYLAGADAPQLAAQVCRVLQDSSVAQRLSISGRHYVQLNHDWSVAAQQLI
                     AAYQRCQEY"
BASE COUNT         1911 a         1529 c         1255 g         1462 t
ORIGIN      
        1 tcgcattaaa aggatcacaa cctaagcccc acacagcttc agtcgggtag gcaatcaccg
       61 caccacgacg aattttttgt gcgatctgtt gaatctgcca agcgctactc attcatttca
      121 ctcagaaatt ttactcaata agtatagcag cagcagactc catctaagta ggtgcatggc
      181 gcttaaaaac atcacaccga ggcagcttta ggccttccat ggcttataca aaatagcagc
      241 aataataagc gctaaagcac cagcaacgat actccacgta atctgcacaa tgcctggtgg
      301 catgggataa attatataag ccgcactact taaagccatt gagctgagta ttagctggag
      361 cttccatggc acatcatgca gaagattttg ctgcttaagt aataaaaaag ccgttattag
      421 agcgccacac accgctgcta aagcaatacc aatcacaccc ataaataaag gtagagcgag
      481 cagcatcaat gcgtttaccg cattgcctgt tagctcacag cgcaatggca gctgcgtatt
      541 gccatcggca taagccaagc gtgccagaag cggattccaa gcaccaaaga ccaagggtac
      601 tgcgaaccaa gccaatagaa gtgcaagtaa agaaatattc tccagctggc gaaacaacag
      661 ctcagacaag gacgggctga tcgcaactaa gccaacagca gcagctaagg caatgcagct
      721 ggtaaccgct aacccctgac gtaaaatcaa caaacgctgt tcaagcaggc gcgaactcaa
      781 gatactcagc agtacctgat ttaaagccat cagtgcaatc aagggtaaat taatcagctt
      841 cctagcaaga ttcacccaag tcaccatacc ctcgcctagc gtgctcgcca ctgcacgttc
      901 gagcaacgta aggccttggc ttgcggcatt actcagtagt aacgggccta atttccccca
      961 aagctcagcg ccaacgcgca gcgctcctcg cattaaccag ggacgccaac ccagcgtgat
     1021 agcacttgga atgagtacgg acagcatggc aaaactgcct aaaataaaag ccaatgatag
     1081 cccttgcgta gtgacttcgc tgcgcgcaat caacaaccaa cccacgggaa acagattaaa
     1141 taaaaacgag cctaaaccgg ccagaatata acgttgctgc gcttgtaaaa tcacggtaaa
     1201 taccgcatgt aaaaaaaata acggcacaca ccaaatcaat aacttaaaat ctgcaactgc
     1261 agcaagcttg gcgctgggct ccagcccagg cccaattaag ttaatccaac ccggtgccag
     1321 cacactgagc atgccagtta agataacacc agcaagcaac agacacgcag caaatgaatt
     1381 gagccattgc ggttgttcac ccgcagtacg ttgttggtat aagggtaatg ctgctgcact
     1441 caaaataccg gcagcaaggg tcatccttag cgcttcagga agaaataacg ctattaaaaa
     1501 tgcatcactt tgtttgccag caccccaagc atcaatcaac aaccattcgc gggcaaaacc
     1561 taatattaag cccgctaaag ttgctaacgt taatagagca gcgctgttaa acatgcgcag
     1621 ttagtgcaac aaagtaaata ggcccttacc accgaccttg taattaagat tatgtgggcg
     1681 ttcacccatc gctttagcac cggtaccacc aataatttta taggcgggaa tttctttagc
     1741 tacaacggta ttggccgtta ctacacagcc acgtctcaat cgagcaccat gggcaatcaa
     1801 cgctcgactc gcgacccaca catagtcttc aatataaata ggcgctccaa cagaccaaaa
     1861 ttctggcgca gtcaagtcat gggaaccagc cacaatcaaa acgtgtgagg caatggccac
     1921 gtgatcaccg atcactaatc ctgcgcgcgc atcaacctgg caatgccaag caatagtact
     1981 atcttcacca agaactaagt tttctactcc cagcacctca gtatggcgcc aaatagtcga
     2041 gcctttacca attttagcgc cgcctaaacg taaccaagcg atgcgtacgg tgtggctggg
     2101 tattttattg ataatgatat tataaacttg ctcccagatt cgtaaacaac ggtcttttac
     2161 agtggcccag tttttcccat aattagggaa aaaatttgag ggaatagtct tttttaaagc
     2221 agcgtaaaac ctttttgcaa tatcattctc aataacatct tcaatatcaa ttgaggatat
     2281 gcttagtatt ttttcattct cttcaaataa aatattatgc tgcagcatat attcaactgt
     2341 tgattgcaat tcctctaaac ttacatttaa aaaaccagcg tattccggca gtttttcata
     2401 cacatcaaaa tctaaataaa cacgatgtct cataatatat tccctcagtt aagcatgtga
     2461 cgttttagac ggaaacaacc taacctgctg caaagaaacg cctactaaaa gccaaaaaag
     2521 cgcaataata acaaaagtaa aactatagta atgatcgaac aaccctgttg ttaatgcagc
     2581 taatacgcca gataggctgc caacccaaat ggcattctca taggttattt tagttatatt
     2641 atttcttggt ttaacctctc gccaccaacg ccaaatcaaa atcgagaaca gcaataaacc
     2701 cggcagtcca attttataaa tgacattcag ccataagttg gaaataccta atagaccact
     2761 ccctggcacg ggcggatcga ctttaaagcc aattccaacc ggataggttg ccatggcttg
     2821 agggaacatg cggtactcat caaagcgtat tccagtactg gcattagatg cggaaaacat
     2881 agacagcaat cgttcttgta atggcgggta aaacatcacc agaagcactg ctagtacagc
     2941 gcctaaggca ataatccgcg cactgtatgg cagcctttta tatgacaacc aaaacaatac
     3001 gagtaacaac gctagaatgg caccacggga aatactaaac agcaggcctg cacagcccaa
     3061 aatagcaacg cttaagccta aaaaacgacg ccatccaggt cgcgtccagc catacaacaa
     3121 acttaaaggc acagttaaag ctaagatccc tccagtcaga tttggatgca cccaaggtga
     3181 agccatccgc tgatagtttg ctgaaaatat atcaatcacc gcttcagggt gtgaatacct
     3241 taaggtttct aacaaaggta taaaggttgt cgcgtcacgg tcttttaaga aatagccaat
     3301 tgagactaag agcatcagca gcgaacctga caaaaaggcc acaatgactt tatccctatc
     3361 ggcatcggtt cgtagtaata atggcactaa aaacagtaca gacaggttta gcatccaacg
     3421 cagccagttg actattgagc tgccttcagc actgaccatc agcactccat aaatcaatgg
     3481 aatcaggctg tatagaaaca aatgcagcat gctcaattca gcggactgcc aactcaaacg
     3541 ttgccgcatc agacctatca gtgcatgcca aaatacaccg gcccaagtca cggctaaaac
     3601 cgcctcacta atggttgtgc gcactccgat ctctatagtt gtataaggaa tgcttgtggc
     3661 taaaactgcg aatagaataa gcccatacaa aggcttgcgc ataacaatta aaaaaaccaa
     3721 tagcgctgcc ataagcacta aaactttagc aggcgttaaa actaatgcag caagcccaaa
     3781 cgataatccg agcagtactg cgagaatacg ctctatggtc attttaggat aactccaaaa
     3841 taagctgatt tacccgcttt tgatacgctg cataagtgta ttgagcagac cactgcgtat
     3901 agtgtatttg gtttttaagt aacggatgag cgcctacttc acgcatacat ttttctaagt
     3961 ctgcaaaaga ataggggtca aaacgcgggc taaattcagg cgttatttca tccaatgcag
     4021 aacccagtgc tgtaacaact ggcgtgccta cacttaatgc ctcaattact ggcaaaccaa
     4081 atccttcagc ataagagggt tgccacagag atgctgcatt gcgatacgct gtgtgtagtt
     4141 gcccttctgt tataccagat agccaatgaa tacctgtcgc atgtctatac tcagggcaat
     4201 cgtctgaact gccaaccagt accaagtcag gcacactcat ttgtgatcta agcatcatcc
     4261 atacttgaat aaataacttg atattctttc tcggttcgcg agtgccgact attagccagt
     4321 agttctgcgg cagctgctca agaggctcag tgtcagcagt aaactcagag actaaattgt
     4381 ttaaaacccg tactttttta cttgcagcag gaaatttatt ttttacctgc tctgcagtaa
     4441 atttagaagg acaccaaatt tgatctgcag catacacaga ccaagcgatt gagagtctat
     4501 ccagtacacg atacacagat gctttaagct tagacgtgtg gtgattatct tcagtgagtt
     4561 gaaataaatc atgcagcaat agagccaaac gcgtgttctt gttttttcga ccgatgggta
     4621 atcccatatt cgctgtggcg atatagagat caattttgtt atcagctaac gcgcggggta
     4681 tgaatcccgc ctcaaagcgt aaacgtttat ggggttgctg cagccctgcc agcggtgttg
     4741 tccattctgg actggttact tttgcgcgta atgggtgatc ttccggaaca acagcaagga
     4801 gcgatagctg agtattgtcg cgtccttgta aaacagcttc tagccctttc acttggcggc
     4861 ctatacctga aaaaggtgcc acgctgacgg gtctataatc aatacctata tgcattttca
     4921 atactcctga cagcgttgat aggctgctat taactgctgc gcagcaaccg accaatcgtg
     4981 attgagctga acgtaatgac gtcctgaaat agataatctt tgagcgacag aagagtcctg
     5041 caacactcga cacacttgtg cagcgagctg cggcgcatca gcacccgcta aataggcgtc
     5101 ctcggcctca atcgataagc ccgatacgcc ttgggctgtt gaaatcaaag gcagtccagc
     5161 tgccaatgct tctagtactt ttagctttga tccaccacca tggcgcaacg gcgccaaaaa
     5221 tacgctgcac tgggactgca gttcaggcaa atcctcgaca aagcctttcc agtcaatgcg
     5281 ctgatccggc caacgttttg cccactcatc tggtagcgca tagcccgcca caataaaacg
     5341 tgcagaaggt actgtttgcc aaacgagcgg cataatctcg gttaaggccc agccaatggc
     5401 gtcgccattc ggcgggtact cataattgcc tacaaataaa accgaaagcg cattaaagtc
     5461 aggctcaact aaggaaaagc tcgctgtatc gacaccattc accaccacat cgactggtgt
     5521 tgcagtgagg cgctggatat tgtctgcatc agtctcggta accgcgagta tgcgctgccc
     5581 ccgagcaaat acatatttct cccacgttcg cgcacgccat tgatcaaaca cgacaaaagg
     5641 tttaaatatt ttcggaaaac gcccgtaggt tgcgccaccc agggatgatt cgaggttgtg
     5701 ctcagtcaaa ataaaagact gttgcctagc ttttaataat tgatcatacg gctggaaact
     5761 atagctgtgc tctatttgaa taacatccca ttgctctgac agcagctcag aaaacaccgc
     5821 ctgcaatggt ttagaaaacc catttacaca ggttaaaata ggccacttgc caaaaaagat
     5881 gcgcaatagc gtcataggat gcttcattga acgccgcggc aaaacaatta aacgctctaa
     5941 atacggagct aatgcaacgt gtgtggcttc atttgccggc tttttacttt gtactaataa
     6001 cgtaatttta aaatcagccg ctgcaagcat tcgtaacaaa tgcaactgcc gtgttttacc
     6061 accactggta gcaggccatg gtaaatacgg taaaatccat aaaatacgca taggctttac
     6121 catattaata cctgcaagct gggcttagtt ttaagcg
//
LOCUS       DASRMS010000153         1237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_911568, whole genome shotgun sequence.
ACCESSION   DASRMS010000153 DASRMS010000000
VERSION     DASRMS010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1237
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_911568"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1237)
                     /locus_tag="VFC74_06735"
     CDS             complement(<1..>1237)
                     /locus_tag="VFC74_06735"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HZK25067.1"
                     /translation="METTSSYISDAQTAHLDAVIDAIEHNNGLPIVYYPKSRSMSGQP
                     RPPSAPAAAPASETTPAAVAFPNPKSQRRPVAIEPLLQLDNQMIANVLLVNTFLGGDT
                     EGRKAKYQQLNIPVLHIVHYRADNYAAYKADSAGVDSFSLPFTLTNAEYIGIQDPVIL
                     TTNEGGEMLPIPEQLNVLVNKAFNLASLQRKDNQDKKLALLFWNHPPGETNQGASNLN
                     VPRSLEKLTSDLQKEGYQVSPVAEQTIIDAVAAMLKPSYRPDHLDELMDTALWSFLPL
                     EAYQAWFKTLPEEVQNEINGYWGEADKYAGLVAYQGQQGFVIPRLESGNLIIMPQPSR
                     GGNDPSQDKDIFHDVKVPMHHYYAAVYLWIREHYQADAIIHFGTHGSQEWLPGKERGL
                     WAYDAPNLAVGSVPVIYPY"
BASE COUNT          310 a          288 c          312 g          327 t
ORIGIN      
        1 atatagggat aaatgaccgg cactgagcca accgctagat tgggtgcatc ataggcccat
       61 aaaccgcgct ctttacccgg caaccactcc tgtgagccgt gggtaccaaa gtgaataatg
      121 gcatcagctt gataatgttc acgaatccat aaatacaccg cagcatagta atgatgcatc
      181 ggcaccttaa cgtcatggaa gatatcttta tcttggctgg ggtcattacc gccacgactg
      241 ggctgcggca tgataatcag attaccgctt tctaaacgtg gaataacaaa gccttgctgg
      301 ccttgatacg cgactaaacc agcatattta tccgcttcgc cccagtagcc attgatttcg
      361 ttctgcacct cttcgggtag cgttttaaac caagcttgat aggcctctag aggcagaaag
      421 ctccacaaag ctgtgtccat taattcgtct aagtggtcag ggcgatagct gggctttagc
      481 attgcagcta ccgcatcaat aatcgtctgt tcagcgactg ggctaacctg ataaccctct
      541 ttttgtaaat cgctggtgag cttttctaaa gaacgcggca cattcagatt agaggcacct
      601 tggttggttt ctcccggtgg gtgattccaa aacaacagtg ctaatttttt atcctgattg
      661 tccttgcgtt gcaggctggc caagttaaag gctttattca ccaacacgtt cagctgttca
      721 gggattggca gcatttcccc gccttcatta gtggttaaaa taaccgggtc ctgaataccg
      781 atatattccg cattggttaa ggtgaagggc aacgaaaaac tatcaacacc tgcactgtct
      841 gccttatagg ctgcatagtt atccgcacgg tagtgaacaa tgtgcaaaac ggggatgttg
      901 agctgctggt acttagcttt acgcccttca gtatcaccgc ccaaaaaggt attgaccaag
      961 agcacattgg cgatcatctg gttgtcgagt tgtaacaagg gttcaatagc gacagggcgg
     1021 cgctggcttt ttgggttagg aaaagccact gctgccggag ttgtttcgct ggcaggggca
     1081 gcggctggtg cgcttggcgg gcgaggctgg ccactcattg agcgagattt aggataatag
     1141 acaatcggca agccattatt gtgttcaatg gcatcaataa cggcatctaa atgcgcggtt
     1201 tgtgcatcag aaatgtagct ggaggtggtt tccatgg
//
LOCUS       DASRMS010000154        11149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_242924, whole genome shotgun sequence.
ACCESSION   DASRMS010000154 DASRMS010000000
VERSION     DASRMS010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 11149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11149
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242924"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="VFC74_06740"
     CDS             <1..213
                     /locus_tag="VFC74_06740"
                     /inference="COORDINATES: protein motif:HMM:NF012779.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HZK25068.1"
                     /translation="PLYKDLKQYRFVTSKAEGVKPYFIYNNAQMKDLISAMPKTTDDI
                     KKISGFGDVKVQKYGKEILEIVNKNR"
     regulatory      431..517
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            968..1870
                     /locus_tag="VFC74_06745"
     CDS             968..1870
                     /locus_tag="VFC74_06745"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK25069.1"
                     /translation="MNKNLKLFVWSMSVLVVLGSLVGCGSQGDTHGNDIYEIKGSDSE
                     VNLVTALVEAYAKGNNEVEFSVTGGGSGTGIAALINNQTDVANSSRQIKEQEVTDAKA
                     QGVEPVPIVFAMDGVAIIVNADNNVKQLTVTEIGNIFAGEITNWKEVGGPDQEITLYG
                     RQSSSGTFSYVMERVVKNDYAQSMKQMNGNSEIVESVANDKSAIGYVALGYIQDATGI
                     KALGVSEDGTDPYVNPSDYQRVKNGEYVLTRPLYQYFNGLPSGALREYVEFELSTSGQ
                     ELVGAEGFLPVTETYRQENEKYLK"
     gene            1913..2791
                     /gene="pstC"
                     /locus_tag="VFC74_06750"
     CDS             1913..2791
                     /gene="pstC"
                     /locus_tag="VFC74_06750"
                     /inference="COORDINATES: protein motif:HMM:TIGR02138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HZK25070.1"
                     /translation="MYGHKDKLIASFLTLIGVFIILLVIGIFGLLISNSFAAWQEIGL
                     RDFLGSTVWNPSGYQSNSYGILGMIASTLLVTAGAMLTAVSVGIACALYLAEIAAPWE
                     REIIKPIVEILAAIPSVVLGFFGIVVLAPLLARLLHLNNGLNALNGSLLLALMSLPTI
                     ISIAEDALNSVPQEYKEASLALGATRMQTLLKVSLPTAASGVTAAVLLGIGRAIGETM
                     TVIMATGNALAFPSGFLSSVRTMTATIAAELGEVPYNSLHYQALFAIGFLLFALSFVI
                     SLVADYFLQRAAERSK"
     gene            2791..3660
                     /gene="pstA"
                     /locus_tag="VFC74_06755"
     CDS             2791..3660
                     /gene="pstA"
                     /locus_tag="VFC74_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HZK25071.1"
                     /translation="MKLNKLMRGKACEQVVFALLRAVMLLVVAVVAYLLYDLVSKGYG
                     VISWEFLTTLPRQGMTAGGILPAILGTCYVTLMTAVAAVPVGICAAIYLHEYARQNLF
                     TRLIRLAIRNLAGVPSVVYGLFGVALFVQALKFGSSILAAGLTLGLLTLPVTITATEE
                     ALKTVPDAYREGALALGAGKWQTISTIVLPSALPGIMTGLILGVSRAAGETAPLLFTG
                     VAFYLPRIPSSLFSQFMALPYHLFILATQHHAITQVRPLAYGTALVLVVLVVLLNLAA
                     IIIRARLRKKLRG"
     gene            3679..4431
                     /gene="pstB"
                     /locus_tag="VFC74_06760"
     CDS             3679..4431
                     /gene="pstB"
                     /locus_tag="VFC74_06760"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HZK25072.1"
                     /translation="MNTLCTENLNLYYGKVQALNNVTINIGKQEVTALIGPSGCGKST
                     FLRTLNRLNDLIDHVTIEGRVLLEGEDIYDKNCNVAALRKRVGMLFQKANPFPKSIYE
                     NVAYGPRIHGLKERRKLDELVEKSLREAALWHEVKHDLNRSALRLSGGQQQRLCLARV
                     LAVEPEVLLMDEPTSALDPIATQKIEDLIRELKEKFTIIIVTHNMQQAARVSEVTAFF
                     LSGEIIEKNKTELLFTHPQDQRTEDYITGKFG"
     gene            4460..5119
                     /gene="phoU"
                     /locus_tag="VFC74_06765"
     CDS             4460..5119
                     /gene="phoU"
                     /locus_tag="VFC74_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015943309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HZK25073.1"
                     /translation="MKQRGEFTKALLALQQDILKMGSLVEEIVAQAVKSLEAQDLQLV
                     AEVFILEKQIDQLELEIEDHCLRLIATQQPMAKDLRKIASAFKIVHDLERMGDHAVDI
                     AKVTQKIGAEPLIKPLIDLPRMALTAQQMIKDALDAYVAEDMAQAETVGQADDTVDSL
                     HQQIFRELLLMMMEKPRVITQATYLLFVSSSLERIADYATNISESVIYLVSGRRLDLN
                     Q"
     gene            5180..5884
                     /locus_tag="VFC74_06770"
     CDS             5180..5884
                     /locus_tag="VFC74_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK25074.1"
                     /translation="MPLILIVDDEEAIIELLTFNLEREGFAVAAAYDGREALSKVAEL
                     KPDLVVLDWMLPELDGLEVCRRLRQDSETALIPIIMLTARGEEIDKILGLELGANDYV
                     TKPFSPRELAARVKAHLRTRQLNQVHNKTAAQTLKRGSLYLDADCYLTEKDRVNIELT
                     PKEFELLFLLASHPGRIFRREVILNHIWGYDYPADTRTVDVHIRYLRQKVEDDPNNPT
                     MLVTVRGVGYKFAEQQ"
     gene            5884..7662
                     /locus_tag="VFC74_06775"
     CDS             5884..7662
                     /locus_tag="VFC74_06775"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25075.1"
                     /translation="MKSIRGKLFATYAGLLLLAVLLLGIGLTWFMRGFYLNTIKSRLA
                     EEAQLTGELLKPLLSDYAVDFKLSETDAYIEYLGAKTNSRITLLSPEGVVIGDSLEPA
                     HLMDNHLTRPEIIAARQDGTGNAIRFSETSNEELLYTAVSLVQNNQVMGYLRLALPLA
                     QLNTALARLRYGLLLGMGLVLLLTLTISYPLAASVTRPLKEIADTAGRIAQGELSSRI
                     ISTQKDEIGGLAAAVNEMAESLQVQLEVVRENRDQLETILTTMVEGIVVFNQDGYAVI
                     VNPAAVEMLNLRPDQWLGKQDLELIRNHALHEKICTVRKKKVYLEHELTLQFPHKRVL
                     MVSLAPVRTNQREAAGVLAVLHDISQLRRLEQVRTDFVANVSHELRTPLTAIRGFAET
                     LANDDKLDDETTARFVQIIWREAQRLQLLIEDILKLSQIESGQITVSKEPVDLQAVIG
                     EVSERLAIPLQEFQFKVNLPEAVPRVKGDSGLLSQAIYNLLDNAIKYTLAGGTITIKV
                     EEQQSMVSLQVSDSGIGIPDEAQERIFERFFRVDSARSRRLGGTGLGLAIVKHIVEAH
                     QGRIELTSQEGVGTTITLLLPLYATS"
     gene            7924..8943
                     /locus_tag="VFC74_06780"
     CDS             7924..8943
                     /locus_tag="VFC74_06780"
                     /inference="COORDINATES: protein motif:HMM:NF021498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Wadjet anti-phage system protein JetD
                     domain-containing protein"
                     /protein_id="HZK25076.1"
                     /translation="MKTVYDLGKKIVTVTEIQEHFKLEKYSQLVEKINRFVAEGTLSE
                     VKNSGSNGMFPALYKRYRVIEDQVDDLSALQEEINYNFPLNFKRDYYLSNLARYREDK
                     EQIEKLAAYFRMSKHLLSEPMAINERSFAIWSQEKFLKEGRGQAILKNLGLTLEDVNV
                     YLTPEPFVYFSCHQQHAHVLIIENKDTWYTLRKLMLQGQASFFTQRIDAIIYGSGKNI
                     EKSLEDYEHTVEEHLLNPAAIFYWGDIDYEGIGIYERLKKRYRDKFEIEILAKAYQAM
                     VDLSQDRVLPKSKDKQNKNIGEIFLKELTSPYRELIVKVLQSGAYIPQEIVNYHNVRE
                     ADTNV"
     gene            8936..10531
                     /locus_tag="VFC74_06785"
     CDS             8936..10531
                     /locus_tag="VFC74_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25077.1"
                     /translation="MYDFLNNFHKRMEKLSYSFLIDSKVSTLTTFKDYFTSLELINLV
                     YSLMCFVLDRSLKDEECTLNRMTVLLAELAANYYDRVLTEDEAHEITRFIVYKILRND
                     GKPFVFTTIDYNSGEKKAFDFHLLQQKTSKADKNKSTFMLTQEGYRLLLGALEVDEKT
                     QIDINQIILELSLKKKNFSQGLLAVENLSNLITAQINVLNNFIYQTRENIFSIEQKAF
                     EDNFLKNIAVLREQNKKFDELKEIIIIEEERLAKTQGEITQENYDSLKQLARIKEFLT
                     AISFKAGELINRHFYFKEEYLRALEESGYYYSHKRINLKEDLIKPIERDASKLGRVYE
                     LFNPLFNSSVPKKFNINYMFNEQKLYLRDDDDDDVLISDEPGQDLELVMRQKNKEDYR
                     GIISSLFSFLVNNNDTDLKSMINYYEHSSQDDYHQLVPSVRKFSEVFIELLRIGSIEI
                     GEMLREQERTYDNEEIEFDLRSLLVDEINAKHRLLTCNLLTFSRSAERERVTVSEQKT
                     AEPGADFITVETLSCPNIIMKLE"
     gene            10540..>11149
                     /locus_tag="VFC74_06790"
     CDS             10540..>11149
                     /locus_tag="VFC74_06790"
                     /inference="COORDINATES: protein motif:HMM:NF039975.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6063 family protein"
                     /protein_id="HZK25078.1"
                     /translation="MYTLEDVRKAIRLYNYLLAHGQLTAEDEQELYHDFTEGKVKEII
                     YTIEEEAQVSIKRFDDTIYLIPDVDNEFLGYKRAELKKEIFPRGDLRNIDLFLAIYIM
                     LQLTAEFYSGKGSNVKIRDLIELGELDEKITERLEVFTSSQDNSVDEETGLAITDIAK
                     YWFALTNEDDAAVFKTRRWYIIQVMNFLKKQDLISIQDETVII"
BASE COUNT         3198 a         2100 c         2741 g         3110 t
ORIGIN      
        1 ccactatata aagatttaaa gcaatataga tttgtaacta gtaaagcaga aggagtaaaa
       61 ccttatttta tttataacaa tgcacaaatg aaagacttga tttctgctat gcctaaaact
      121 acagatgata ttaaaaagat atctggtttt ggagatgtta aagtccagaa gtatggaaaa
      181 gagattttag aaatagtcaa taaaaacagg taatgaaaaa cttataccac atcagtatcc
      241 agatttagaa gcaaaaatgt atatcccagc ttattgttca acgacttaat ggctggggtg
      301 tgtattttgg tattttatta ctgtttaggg atcgaaaaag ctccttttct tatgtatgat
      361 ggaagccatt aggggattgt gtataattat agttgtaaac tgacaaacca tgatagatta
      421 atacagggtc actcgatatt agcaaaccca gcgaaagttg gggacgcaaa gcagatggtc
      481 tacggcacgt gtgtgctatg acagtctggc taccgaaggt gcatttttat tggggtgacc
      541 ttgcgtttga atacatacat gcttgcaatg acttctggta gaaacatcaa gcgatagata
      601 cgaaaaaata tgagtttttt attggcgggc gttctggtta acagatgttc tgcctttttc
      661 tcatgccaaa aagaatattg tgctttttaa catgttgatt cgatggtagt taaatagtca
      721 gagatctaag cgtacataaa ttgaatacgc tgggtcggat tgtgtagttt gaccgcagta
      781 attgtgcact atgatctgtc ggcgaaatcc ctctgtataa tggatttaat aaccttgtta
      841 aaataaggtt cattctgcct tgttgtctgc tgcaggtgat tttaaccttc atttaacatt
      901 gcctgccttt tgctttaaca ttggcttgtt ataatcaggc tgaagataaa tcaagaggag
      961 gttaataatg aataaaaact tgaagctatt cgtgtggagt atgagtgtac tggtggtgct
     1021 tggcagcttg gtgggctgtg gcagtcaagg tgacacacat ggcaacgata tttatgagat
     1081 taaggggtct gacagtgagg taaatttagt gactgcttta gttgaagcgt atgcgaaagg
     1141 gaataacgag gtagaatttt ccgttaccgg cggtggttcc ggcaccggca ttgccgcctt
     1201 aatcaacaac cagactgatg tcgcaaattc gtctcgccag attaaagagc aggaagttac
     1261 ggatgctaag gcgcagggtg tagagccagt accgattgtt tttgctatgg atggcgtggc
     1321 gattattgtt aatgctgata acaatgtgaa gcagttaact gtgacagaaa tcggcaatat
     1381 atttgctggg gagatcacca actggaaaga agtgggtggt cctgaccagg agattacttt
     1441 gtatggtcgt caaagcagtt ctggtacctt tagttatgtg atggagcgag tggtgaaaaa
     1501 tgattatgcc cagtctatga agcagatgaa tggtaattca gagattgtgg aaagtgtggc
     1561 aaatgacaaa tcagcaattg gctatgtggc tctggggtac attcaggacg ccacgggcat
     1621 caaagctctt ggtgtgtcag aggatggtac tgatccttat gttaatccca gcgattacca
     1681 gagagtcaaa aacggtgagt atgtcttgac acgaccgctt tatcaatact ttaatggcct
     1741 gccgagcgga gcgctgcgtg agtatgtgga gtttgaatta agcaccagcg gacaggaatt
     1801 agtgggagca gagggatttc taccggttac ggagacctat cgccaagaaa acgaaaaata
     1861 cctaaagtag ggtactcctc ctattgccaa ctatcagagg ggagaactgc agatgtatgg
     1921 tcacaaagat aaactaattg cttcttttct aactttaatc ggtgttttta ttatcttgct
     1981 tgtcattggc atttttggtt tgctgattag taatagtttt gctgcctggc aagaaatagg
     2041 tttgcgtgat tttttaggca gtactgtttg gaatccgtcg ggttatcagt ctaatagcta
     2101 tgggatctta ggcatgattg ccagcacctt actggtaact gccggggcaa tgctgacggc
     2161 ggtatcagtg ggtattgcct gcgctcttta tttagcagaa attgctgctc cctgggagcg
     2221 tgaaataatt aagccgatag tggagatctt agcggccatt ccttcagttg tgctgggttt
     2281 ctttggcatt gttgtcttag cgccgctgct tgcccgcctc ttacatttaa acaatggctt
     2341 aaacgcttta aatggttcgc tgctgttggc gctgatgtct ctgccgacaa ttatcagtat
     2401 agctgaagat gccttaaaca gcgtgccgca ggagtataag gaagcttctt tggccttagg
     2461 cgcaacaagg atgcagacgc tgctcaaggt gtctctgccg acggccgcgt ccggggtaac
     2521 ggcggctgtg ttgctgggga ttggccgcgc cattggtgag acaatgactg tcatcatggc
     2581 cacaggcaat gctttagctt tcccctccgg ctttttgagc tcggtgcgca cgatgacagc
     2641 aacgatcgcc gcggaacttg gtgaggtgcc ttataacagt ctgcactatc aagctttgtt
     2701 cgccattggt tttctgcttt ttgcgctaag ttttgtcatc agtttggtgg ctgattattt
     2761 cttacaacga gcagctgaga ggagcaaata atgaagctaa acaaattaat gaggggtaaa
     2821 gcttgtgaac aggttgtttt tgccctcttg cgagctgtta tgctgctggt ggtagcggta
     2881 gttgcttatc ttctctacga tttagtcagc aagggatatg gggtgataag ttgggaattc
     2941 ctcacaactt taccgcggca agggatgacc gcaggaggaa tcctgccggc aatcttgggc
     3001 acttgctatg tgaccctgat gacggccgtg gcagcggtgc cggttggtat ctgtgctgct
     3061 atttatctcc atgaatatgc ccggcaaaat ctttttacac gtctcatccg tttggcgatt
     3121 cgcaatctgg ccggcgtacc ttcggtcgtc tatggtctgt ttggggttgc cctctttgtg
     3181 caggcgctta agtttggctc atcgattctg gcggcaggtt taaccttagg gttactgacg
     3241 ctgccggtaa cgattaccgc cacggaagaa gcgctgaaga cagtgcctga tgcttatcgg
     3301 gaaggagcgt tggccttagg tgcgggtaaa tggcaaacta ttagcaccat tgtcttgccc
     3361 tcagccctgc ccggcattat gacgggctta atcttaggtg tttcacgggc cgctggggaa
     3421 actgctccct tgctttttac cggtgtagct ttttatttgc cgcgtatccc cagctctctt
     3481 ttttcccaat ttatggcctt gccctatcac ctgtttattc tggccaccca gcaccatgcc
     3541 attacacaag tacgtcctct agcttacggt acagcgttgg tcttagttgt tttggtagtg
     3601 ctgcttaatt tagcagcgat cattatccgg gctcgactgc gaaagaaact gcgagggtag
     3661 gtggaagata tgtcagagct gaatacacta tgtacggaaa atttaaattt gtattatggc
     3721 aaagtgcagg cactaaacaa tgtgaccata aatattggca agcaggaagt aaccgccttg
     3781 attggcccgt ctggctgcgg taaatctacc tttttacgca cgctaaaccg tttaaatgat
     3841 ttgattgatc atgtaactat tgaggggcgt gtccttttgg agggagagga catttatgat
     3901 aaaaactgta atgtggcagc tttacgaaaa agagtaggca tgctttttca aaaagcgaat
     3961 ccttttccca agtctattta tgagaatgtg gcctacggac cacgtattca tggcttaaaa
     4021 gaacgtcgta aactggatga gcttgtggaa aagagtttgc gggaagcagc tttatggcat
     4081 gaagttaagc atgatttaaa tcgttctgct ttacgtctgt ccggcgggca gcagcagcgg
     4141 ctttgtctgg cccgggtgct ggcagtagag ccggaagtac tgctgatgga tgagcctacc
     4201 tcggcgcttg atcccattgc cacgcaaaaa attgaagatt taatccgcga attaaaagaa
     4261 aaattcacga ttattattgt gacacacaat atgcagcagg cggctcgcgt ttctgaggta
     4321 acagcctttt tcttaagcgg cgagattatt gaaaagaaca agacagagct gctctttact
     4381 cacccccaag accagcggac agaagactat atcactggta aatttggtta ggccaaatca
     4441 gactgaggag gatgtcaacg tgaaacaacg tggtgagttt acaaaagcgc tgctggcgct
     4501 gcagcaggat attttaaaga tgggtagttt agtggaagag attgtggcgc aggcagttaa
     4561 atccttggaa gcgcaagatt tacagctagt ggctgaggtg ttcatactgg aaaagcaaat
     4621 tgaccaattg gagctggaga tagaagatca ttgcctgcgc ttgatagcga cccagcagcc
     4681 gatggccaaa gatttgcgaa aaattgcctc tgcttttaaa attgtgcatg acttggaacg
     4741 tatgggcgac catgctgtgg atattgctaa agttacccag aaaattgggg cagaaccgct
     4801 gattaaaccg ttgattgatt taccgcgcat ggctctgaca gcgcagcaga tgattaaaga
     4861 tgcccttgat gcttatgtgg ctgaggatat ggctcaggct gaaacagtgg ggcaagctga
     4921 tgatacagtc gattctttgc atcagcagat ttttcgtgaa ctactgttaa tgatgatgga
     4981 aaaaccgcgg gtgataaccc aggctactta tcttctattt gtcagcagct cgctggaacg
     5041 tattgctgac tatgctacca atattagtga aagtgtcatc tacttagtta gcggccgccg
     5101 ccttgatttg aatcagtaga attttgtttg tccgtttcgt gataaaatgc tagaatgtga
     5161 gagaaggggg gttaccaggt tgccattgat tctgatagtc gatgatgaag aagccatcat
     5221 tgaactcctt acttttaatc tggaacggga aggctttgca gtggctgctg cttacgatgg
     5281 tagagaggct ttaagtaagg tagctgagct gaagccggat ctggtggtgc tggattggat
     5341 gctgccggaa ttagatggtt tagaagtctg ccgccgcctg cgtcaggaca gcgagacagc
     5401 acttatcccg attattatgc tcacggcacg cggtgaagaa atagataaaa ttcttggttt
     5461 ggaacttggc gctaatgact atgtaacaaa acccttctct ccccgcgaat tggcagcccg
     5521 cgtcaaagct catttgcgga cacgccagct taaccaggtg cacaataaga cggcagccca
     5581 gactttaaaa cggggcagct tgtatttaga cgctgattgt tacttaacag agaaagaccg
     5641 agtcaacatt gagctgacgc ccaaagagtt tgagttatta ttcttactgg cgtctcaccc
     5701 tgggcgtatc tttcggcggg aagttatttt aaaccacatt tggggttatg actatcctgc
     5761 agatacacgt actgtggatg tccatatccg ttatttgcgc caaaaagtag aggatgaccc
     5821 caacaatccc accatgctgg tgactgtacg gggcgtaggc tataaatttg cggagcagca
     5881 gtgatgaaga gcattcgggg taagcttttt gctacttatg ccggactcct gctgttggct
     5941 gtgctgctgc tgggtatagg attaacatgg tttatgcgtg gtttttatct gaataccatt
     6001 aaaagccgtt tagcggaaga agcacaactt acaggtgaac ttttaaaacc cttactgtct
     6061 gactatgctg ttgatttcaa gctgagtgaa actgatgcct atattgaata cctgggtgca
     6121 aaaaccaaca gcaggattac tctgcttagc ccagagggcg tggtcattgg cgattctctt
     6181 gagcccgccc acctcatgga taaccacctg acacgaccgg aaatcattgc cgcccgtcaa
     6241 gatggtactg gtaatgctat ccgtttctct gagaccagca acgaagaatt gctttatact
     6301 gctgtttccc tcgtccaaaa taaccaagtt atgggttatt tgcgtttggc gctgcctcta
     6361 gcgcagctga atactgcttt agctcgttta cgttacggct tattattggg catggggttg
     6421 gtgcttttat taacgctgac tataagttat ccgctagccg ccagcgtaac gcgtccatta
     6481 aaagaaatag cggatacagc aggacggatt gcgcaaggtg agctgtcaag ccggattatt
     6541 agtacgcaga aagatgaaat aggcgggtta gctgctgcgg tcaacgagat ggccgaatcc
     6601 ctgcaagtac agttggaagt ggtacgagaa aacagagatc agttggaaac aattttaacc
     6661 acgatggtgg aagggattgt cgtttttaat caggatggtt atgccgtgat tgttaatcca
     6721 gcagcagttg agatgctaaa tttaaggcct gatcaatggt tggggaaaca ggatttggag
     6781 ctaatccgca atcatgcttt gcatgaaaaa atctgcacgg tcagaaagaa gaaagtatat
     6841 ctagagcatg agctaacatt gcaatttccc cataaacgag tgctgatggt cagtctggca
     6901 ccagttcgca ccaatcagcg cgaagctgct ggggttttgg cagttttgca tgatatcagc
     6961 cagctgcggc ggttggaaca agtacgaacg gattttgtgg ccaatgtctc ccatgaactg
     7021 cggacgccgc tgacagctat tcgcggtttt gcagaaacgc tagctaatga tgacaagctt
     7081 gatgacgaga caacggcacg ctttgtgcaa atcatctggc gagaggcaca acgcttgcag
     7141 ctgctcattg aagatattct taaactttcg caaattgaaa gtggtcaaat tactgtgtcc
     7201 aaagaacctg tagacttaca agcagtgatt ggggaagtca gtgaacgttt ggctatcccg
     7261 ctgcaggaat ttcagtttaa agtaaactta cctgaggctg ttcctagagt aaaaggagac
     7321 agcggcttat taagtcaggc gatctataat ctgttggaca atgctattaa gtacaccctg
     7381 gcaggcggca caattacaat caaggtagag gaacagcaga gtatggtctc tctgcaggtg
     7441 agcgacagcg gcatcggtat tccggacgaa gcacaagaac gtatctttga gcgttttttc
     7501 cgagtagact ctgctcgttc gcggcgttta ggcggcaccg gactgggttt ggccatagtt
     7561 aaacatatcg tggaggcgca tcaggggagg atcgaattaa ctagtcaaga gggtgtgggc
     7621 acaacaataa ccttgctgct gcctctgtac gctacaagct aattaaaaat ttgcttttcc
     7681 agtactcccg ttaaacctcc gggcagaccc tgattctctg aaaaaagtaa gtatctgcca
     7741 gatattaaaa cgacgcaccg agttattagt gcgtcgtttt aatatctacc ttggtttatg
     7801 gcgtaagcac tagttgtacg tacagaattt tttagttaat aggtattttg tcttttatct
     7861 tgccaggaaa tgaacaaata ctatattata aggataaagg tgtcagaaaa tggagttggg
     7921 gcaatgaaaa ctgtttatga cttgggtaaa aagatagtta ctgtaacaga aattcaagag
     7981 cactttaagc tagaaaaata ctcacagtta gttgaaaaaa taaacagatt tgttgcggaa
     8041 ggtaccttga gcgaggtaaa aaacagtggc tctaatggta tgtttccggc cttatacaag
     8101 agatacaggg tcatagagga tcaggttgat gacttatctg ctttgcagga agaaataaac
     8161 tataattttc ccctgaactt taaaagagat tactacttgt ctaatttggc aaggtaccgt
     8221 gaggataaag agcaaataga aaaattagca gcatacttca ggatgtctaa gcacctgttg
     8281 tcagaaccga tggccattaa tgaacgaagt ttcgccattt ggagccagga gaagttttta
     8341 aaagaaggca gaggacaggc catcttaaag aatttgggac taactctaga ggacgtaaat
     8401 gtttatctaa cccctgaacc gtttgtttat ttttcctgcc atcagcagca tgcccatgta
     8461 ctgatcatcg agaataaaga tacctggtat acgctgcgga aactgatgct gcaagggcaa
     8521 gcttcatttt ttacgcaaag gatagatgct attatttatg gcagcggtaa gaatatagaa
     8581 aaatcccttg aggactatga gcatactgta gaagaacatc tgcttaaccc tgcggccatc
     8641 ttctattggg gagacattga ttatgagggt attggcatct atgaaaggct taaaaaaaga
     8701 tatcgtgata aattcgagat agaaattttg gcaaaggcct atcaggcgat ggtggatctg
     8761 tctcaagata gagtgctgcc taaatctaaa gataagcaga ataaaaatat tggggagatc
     8821 ttcttaaagg aactgacatc cccctatcgt gagttaattg tcaaagtact gcaaagtggt
     8881 gcttacattc cgcaagaaat agttaactat cataacgtaa gagaggcaga tacaaatgta
     8941 tgattttctg aataatttcc ataaacggat ggaaaagctc tcttactcct ttttaattga
     9001 cagtaaggtt tccaccttaa ctacctttaa agactatttt accagcctgg aactgatcaa
     9061 tttagtctac agcctgatgt gttttgtcct agacagatca ttaaaggatg aggaatgcac
     9121 cttaaatagg atgactgttt tgttggcaga gctggcggct aattattatg acagagtatt
     9181 gacagaagac gaagcgcatg agatcacgcg ttttattgtg tacaaaattt tgcgcaatga
     9241 tggtaagcct tttgtcttta caacaattga ctataacagc ggggaaaaga aagcttttga
     9301 ttttcacctg ctgcagcaaa aaacctcaaa ggcagacaaa aataaaagta cctttatgct
     9361 gacccaggag ggctacaggc tgctgctggg ggctttagag gtagatgaaa aaacccagat
     9421 tgatatcaat caaatcattt tagagttaag tttgaaaaag aagaattttt ctcaggggct
     9481 tttagcagtg gaaaacctga gtaacctgat tacagcgcaa attaatgtct taaataattt
     9541 catctatcaa acgcgagaaa atatcttctc catagagcaa aaagcatttg aagataattt
     9601 cctcaaaaac attgctgttt taagagaaca gaataagaag tttgatgaat taaaggaaat
     9661 catcatcatt gaagaagaga gattagctaa aacgcagggc gaaattactc aggaaaatta
     9721 tgattccctg aagcagttgg cgagaataaa agagtttttg accgcgatat cttttaaggc
     9781 gggtgaactg attaaccgtc acttttattt taaagaagaa tatctgcggg cactggaaga
     9841 gtctggttac tattacagcc ataaacgcat caatctaaaa gaagatttga ttaaacccat
     9901 agagcgggat gcctccaagc ttggcagagt ctatgaacta tttaaccctc tatttaattc
     9961 cagtgtgccg aaaaagttta atatcaatta tatgtttaac gaacaaaaat tgtacttgcg
    10021 agatgacgat gacgacgacg tactgatcag tgacgaaccg ggacaagact tggagctggt
    10081 gatgcggcag aaaaacaaag aagattatcg tggcataatt tcctccctgt tctcattttt
    10141 agtcaacaat aacgatacgg atttaaaaag catgatcaac tactatgagc atagcagcca
    10201 ggacgactat catcagctcg taccctctgt gagaaagttt tcagaggttt ttattgagct
    10261 gctgagaatc ggcagcatcg agataggcga aatgttaagg gagcaagagc ggacctatga
    10321 taacgaggag atagaatttg acttaaggtc actgctggtg gatgaaataa atgccaagca
    10381 ccggctttta acctgcaact tactcacctt tagcagatct gccgagcggg aaagagtcac
    10441 agtaagtgag cagaaaacgg ctgaacctgg agcagatttc atcaccgtag aaaccttaag
    10501 ctgccctaat attattatga aactggagta gataaagcga tgtatacatt agaagatgtt
    10561 agaaaggcaa tacgactgta taattatttg ctagctcatg gccagttaac cgcagaggat
    10621 gagcaggagc tttatcatga cttcacagag ggtaaggtta aagaaattat ttataccatt
    10681 gaagaagagg ctcaagtcag cattaaaaga tttgatgata ctatttatct catccccgac
    10741 gtggataacg agtttctagg ctataaaagg gcggaattaa aaaaagagat cttcccccgg
    10801 ggcgatctga gaaatattga tctctttctg gctatttata ttatgctaca gcttaccgca
    10861 gagttttata gtggcaaggg cagtaatgtc aagattagag atttaattga acttggtgag
    10921 ctagatgaaa aaataactga gcggttagaa gtgtttacat ctagtcaaga caactccgta
    10981 gacgaagaaa cgggtttagc catcacagat attgctaaat actggtttgc cttaacgaac
    11041 gaagatgatg ctgctgtctt taaaacaaga agatggtata ttattcaggt catgaacttt
    11101 ctaaagaaac aagatttaat cagcattcaa gatgaaacag taattattc
//
LOCUS       DASRMS010000155         1159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_311448, whole genome shotgun sequence.
ACCESSION   DASRMS010000155 DASRMS010000000
VERSION     DASRMS010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_311448"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VFC74_06795"
     CDS             <1..502
                     /locus_tag="VFC74_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002572462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25079.1"
                     /translation="TALNGKVDKVSGKGLSTEDYTSAEKTKLAGIAAGAQVNTVTSVA
                     GKTGAVTLTKADVGLGNVDNVKQATKAEFDSHNGDTIKHITAAERTGWNAKTGKYTAN
                     IGNGTATEFTLTHNLNTKDLTVGIEEVATGEMVWTDIVKISVNAIKVLFAQAPASNQY
                     RATVVG"
     gene            515..>1159
                     /locus_tag="VFC74_06800"
     CDS             515..>1159
                     /locus_tag="VFC74_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002588792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25080.1"
                     /translation="MKYVGKTDLKIGNNAVWHGGNFVPDDKADKSHGHTKSQITDFPS
                     SLPANGGTANKANILVITDTRDIDDTPGDLQKKGFTGAFKRRATVNNPPVGSSNNYVF
                     IMTVAGYGTASDGSGGWPIQVAFGIEGLAYRRGTSSTAWGPWDRISNLSDIPTSLPAN
                     GGNAATVNGKTVAENVPAGAKFTDTTYSEITTSEIDAGTSSTLRTISGRRVKYIL"
BASE COUNT          393 a          229 c          296 g          241 t
ORIGIN      
        1 gacagcttta aacggaaagg ttgataaggt aagcggtaag ggactttcca cggaagatta
       61 caccagcgca gaaaagacca aactagccgg tatcgcagca ggggcgcagg tcaacacggt
      121 aacgagtgtg gcgggcaaaa ccggggccgt tacgcttact aaagcagatg tagggttagg
      181 taatgtggat aatgtaaagc aagctacaaa ggccgaattt gacagccaca acggggacac
      241 aatcaagcat attacagcag cagaaagaac aggctggaac gctaaaacag gcaaatatac
      301 cgccaatatt ggcaatggta cagccacgga attcacgttg acgcataatc taaacacaaa
      361 agatcttact gtaggtatcg aagaggtggc cactggggaa atggtttgga cggacattgt
      421 taaaatcagt gtaaatgcca taaaggtgct atttgcccaa gcaccggcaa gcaaccaata
      481 ccgggcaacg gtagtaggat aggcaggtga tatagtgaaa tatgtaggta aaacggattt
      541 aaaaatcggg aataatgcag tttggcatgg gggcaatttt gttccggacg ataaggcaga
      601 caaaagccac gggcatacaa aaagccaaat aacagatttc ccttcttcct tgcccgctaa
      661 tggagggact gcgaacaaag ctaatatatt agtgattaca gatacacgag atattgacga
      721 taccccaggt gatttgcaaa agaaagggtt tacaggcgca tttaaaagac gagctacagt
      781 taataaccca cccgttggta gcagcaacaa ttatgtgttt ataatgacag tggcgggtta
      841 tggaacagcc tcagatggtt caggcggctg gcctatacag gtagcattcg ggatagaggg
      901 gcttgcctat aggaggggaa cctcaagcac agcctggggg ccatgggaca gaatatcaaa
      961 tctttcagat attccaacct cactgccagc caatggtgga aatgctgcca cagtaaacgg
     1021 caaaacagta gcagaaaatg ttcctgctgg ggcaaaattt actgatacaa cttacagcga
     1081 aataaccact tcagaaattg atgcaggaac ttcttctact ttaagaacaa tatcaggtag
     1141 aagagtgaaa tatatactc
//
LOCUS       DASRMS010000156         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_580100, whole genome shotgun sequence.
ACCESSION   DASRMS010000156 DASRMS010000000
VERSION     DASRMS010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580100"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2455
                     /locus_tag="VFC74_06805"
     CDS             <1..>2455
                     /locus_tag="VFC74_06805"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25081.1"
                     /translation="TFVMPAKDVTVTANYLDIGKLVGNVTITGETKFGETLTATVTDS
                     NAKTQFNYKWRRSASNGVVIGSNSPTYQLTVKDIPYHIYCEITTEGKTNSIFGFTLTS
                     IRKADGPAAPTGLGSTAETVAGKKDGTITGVQLGQEYALADGGEWKDVQGTSVINLPA
                     GDYEVRVKETQTHKPGAVAKITIEPGEPVPTYAVTVTGGTGGGNYEVGSMVTITADAP
                     AKGKVFDQWIATGLTDDYELESDPLRFTMPSNDVTLKATYKDAPATTYAVTVNSGTGS
                     GNFAEGETVNITANTADAGKVFDKWTTTDGVAFASANSATTTFVMPAKNVTVTATYKD
                     APVSTYAVTVNSGTGSGDYSQGDTVTIKANEPEIGKVFDKWTTTDGVTFTDANSATTT
                     FVMPAKAVTVTATYKDAPVTTYVVTVTDGTGSGDYAEGATVNITANAPAAGKVFDKWT
                     TTDGVAFANANSATTTFKMPAKGVVVTATYKDAPVTTYTVTVSNDGNGTGTAKPATAE
                     AGTEITLSASASSGYQFKEWQVVSGGVTISSDKFTMPAGNVEVKAIFEVLPPTLTGVS
                     ISPKTTSVQKGTSDTFTAVVTGTGAFDSTVTWSVLGGIPGTNIDNNGKLTVDASETAT
                     TLTVTATANGDSSKSDSATVTITDVAITKYAVTVNNGTGGGNFAEGETVSITAGAPAD
                     GKVFDKWTTADGVTFTDANSATTTFTMPAKAVTVTATYKDAPATTYAVTVNNGTGGGN
                     FAEGATVSITAATPATDKVFDKWTTSDGVTFANANSVTTTFTMPAKAVTVTATYKDAP
                     ATTYAVTVNNGTGGGNFAEG"
BASE COUNT          806 a          512 c          578 g          559 t
ORIGIN      
        1 acttttgtta tgccagctaa agatgtaacc gttacagcca actatttaga tattggtaag
       61 ctagttggca atgtgacaat tactggagaa actaagtttg gcgaaacttt aacagcaaca
      121 gtaacagact caaacgccaa aactcagttc aactacaagt ggagacgtag cgcctccaat
      181 ggagtagtaa ttggaagcaa tagtccaact tatcagctga ctgttaaaga tattccgtat
      241 cacatttact gtgagattac aaccgaaggt aagacgaact caatcttcgg cttcactttg
      301 acgtctatta ggaaagccga cggcccagca gcccctactg ggttgggaag caccgctgaa
      361 acagtagccg gtaaaaaaga tggaacaata accggtgtac aacttgggca ggagtatgcg
      421 ctagctgacg gaggcgaatg gaaagacgtt caaggaacat ccgttattaa cctaccagca
      481 ggcgactatg aggtgcgtgt gaaagaaaca caaactcaca agcctggagc tgtggcaaag
      541 ataacgattg agccgggaga gccagtcccc acctatgcag ttaccgtaac tggcggtaca
      601 ggcggcggaa actatgaagt gggttcaatg gtcaccatta cagcggatgc tcctgctaaa
      661 ggcaaggtat ttgaccagtg gatagcaaca ggtcttactg atgattatga gcttgaatca
      721 gatccattga gattcacaat gccatccaat gatgttactc ttaaagcaac ttataaagat
      781 gcacctgcta ccacttatgc agttactgta aatagtggga caggcagcgg aaactttgca
      841 gaaggtgaaa cggttaacat tacagcaaat actgctgatg caggaaaggt atttgacaag
      901 tggacgacaa cagatggagt agcatttgca agtgcaaata gtgctactac aacgtttgtt
      961 atgcctgcta aaaatgtcac cgttaccgca acttataaag acgcacctgt tagcacctac
     1021 gcagttactg taaatagtgg tacaggcagc ggagactatt ctcagggaga cactgtcact
     1081 attaaagcaa acgaacctga aataggtaag gtgtttgaca agtggacaac aacagatgga
     1141 gtaacattta cagatgcaaa tagtgctacc acaacatttg ttatgccagc taaagcggtc
     1201 accgttacag ccacttataa agatgcacct gttaccacct atgtagttac agtaactgac
     1261 ggtacaggca gcggagacta tgcagaaggt gctacggtca atatcaccgc aaatgctcct
     1321 gctgcaggca aggtttttga caaatggacg acaacagatg gagtagcatt tgcaaatgca
     1381 aatagtgcca caacaacttt taaaatgcct gctaaaggtg tcgttgttac agcgacctat
     1441 aaggacgctc cagttactac ttatacagta actgtaagca acgatgggaa tggaacaggt
     1501 acagcaaagc cagcaacagc agaagcagga acagagataa ctttatctgc aagtgcaagt
     1561 agtggatatc aatttaaaga atggcaagta gtaagtggag gagtaacaat ctcaagcgat
     1621 aaattcacta tgccagcagg aaatgtagaa gttaaggcga tatttgaagt attaccacca
     1681 accctaacag gtgtatctat ctcacctaaa actacatcag tgcaaaaagg tactagtgac
     1741 accttcaccg cagtagtgac aggtacaggt gcatttgata gcacagtgac ttggagtgtt
     1801 ttaggtggta tcccagggac taatattgac aataacggaa agcttacagt agatgcatca
     1861 gaaacagcaa caacactaac ggtaactgcc acagcaaatg gcgatagcag taagagtgat
     1921 agtgcgacag taactattac tgatgtagca attactaaat atgctgtaac cgtaaacaat
     1981 ggtactggtg gtggaaactt tgcagagggc gaaactgtta gtatcacggc gggtgctcct
     2041 gctgacggca aggtatttga caagtggaca acagcagatg gtgtaacatt tacagatgca
     2101 aatagtgcca ccacaacctt cactatgcca gctaaagcag ttaccgtcac agcaacttat
     2161 aaagatgcac ctgctaccac ttatgcagtt actgtaaata atggtacagg cggcggaaac
     2221 tttgcagagg gcgcaactgt cagtatcacg gcggccactc ctgcaacaga caaggtgttt
     2281 gacaagtgga ccacatcaga tggagtgaca tttgccaatg caaacagtgt taccacgaca
     2341 ttcactatgc cagctaaagc tgttaccgtc acagcaactt ataaagatgc acctgctacc
     2401 acttatgcag ttactgtaaa taatggtaca ggcggcggaa actttgcaga gggcg
//
LOCUS       DASRMS010000157         1181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_779270, whole genome shotgun sequence.
ACCESSION   DASRMS010000157 DASRMS010000000
VERSION     DASRMS010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779270"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..>1181
                     /locus_tag="VFC74_06810"
     CDS             8..>1181
                     /locus_tag="VFC74_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HZK25082.1"
                     /translation="MSFNAIISADVKNFEAGIDKAIGDIDKLAKTASEKLERIGKSFE
                     NAGKKASIMSAAIVAAGGVMVSSFITQEKAERKLSAAINATGGDVEVLSARFKKFASD
                     LQAVTLTGDETTLGMLQMAQTMGLTAVQSERAVKNAIAMESAFGVSAASAMRYTAALE
                     DGDATMLKRYIPALKSIEDESLLAAKAQEILGNAFEVAKEDAQTFGGSFTQLKNSAGD
                     LMEEFGAIISKALMPLVEWAKNAVEHFSNLDEGTKELIVTVGGIVAAIGPLLLILGTI
                     VGKVVPAMLVGFKALRTVVMATSGAFAKLGVIIAANPIGAIAVGITAVIAALVIFNRR
                     LSDTSTLSDKIMTDALKNTAGERVEVERLLRVLNSEKATREQKEKVLRKLNEISPEY"
BASE COUNT          360 a          213 c          325 g          283 t
ORIGIN      
        1 agaaagaatg agttttaatg caatcatatc ggcagacgta aagaactttg aggctgggat
       61 agataaggcg attggtgaca ttgacaaatt agcgaaaaca gcaagcgaga aacttgaaag
      121 gataggcaaa agttttgaga acgctggcaa aaaggcttct atcatgtcgg cagcaatcgt
      181 tgcggctggt ggtgtgatgg ttagcagttt tataactcaa gaaaaagcag agcgtaaact
      241 atccgctgca attaatgcca caggcggaga cgtcgaggta ctatcagcaa ggtttaagaa
      301 gtttgctagt gacttgcaag cggttacgct gacaggtgat gaaaccacgt tgggtatgct
      361 gcagatggct cagacgatgg gcttgacggc tgtacaatca gagcgagccg ttaagaatgc
      421 aattgccatg gagtcggctt ttggcgtttc ggcagcaagc gcgatgcggt acacggcagc
      481 attagaggat ggcgacgcaa ctatgctaaa gcgttacata ccagcattga aaagtattga
      541 ggatgaatcg ctattagcag cgaaagcaca agagatttta ggcaatgctt ttgaagtggc
      601 aaaggaggat gcccaaacct ttgggggtag ttttacccaa ttaaaaaact ccgcaggtga
      661 cctaatggag gagtttggcg caattatttc aaaagcctta atgccgttgg tcgaatgggc
      721 taaaaacgca gttgaacatt tcagcaattt agacgaaggc acgaaagaac taattgtaac
      781 cgttggtggg attgttgctg caattggccc gcttttgcta atattaggta caattgtagg
      841 caaggtcgtg ccagcgatgc ttgtcgggtt caaagcgtta aggacggtgg taatggccac
      901 gagcggagcg tttgctaaac tgggagttat aatagcagcc aaccctatcg gcgctattgc
      961 cgtagggatt accgctgtta ttgcagcctt agtaatattt aaccgccgac tttcagatac
     1021 gtccacttta tctgataaga taatgaccga tgcgctgaaa aatacagcag gcgaaagggt
     1081 tgaagttgaa cgcttgttga gggtactgaa tagcgaaaaa gccacaaggg agcagaaaga
     1141 gaaagtttta agaaaactga acgaaatttc tcccgagtat t
//
LOCUS       DASRMS010000158        16518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_644752, whole genome shotgun sequence.
ACCESSION   DASRMS010000158 DASRMS010000000
VERSION     DASRMS010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 16518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16518
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_644752"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(28..408)
                     /locus_tag="VFC74_06815"
     CDS             complement(28..408)
                     /locus_tag="VFC74_06815"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK25083.1"
                     /translation="MAPNQKAEKQHDLLPYKGKIERFIEPCLLLLLAEKPAHGYELIE
                     PLSQFDFEPNVDPGQIYRTLRRMEKHGFVVSNWETGKAGPARRRYQLTAQGEGFLTSW
                     IKTLTKRVQMIEKLLQRYDQIMER"
     gene            582..2123
                     /locus_tag="VFC74_06820"
     CDS             582..2123
                     /locus_tag="VFC74_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HZK25084.1"
                     /translation="MTYLLRVNMSTQAVTTSELPPDYQRLGGRALTSRICQDEVDPFC
                     YALGERNKLVIAPGLLAAADFPYGGRIAMAGKSPLTQGLTASNAGGLTAARLGALGIQ
                     AIVVEGLPQDDKLYVLVISEKEARLVPTPELRGKGTSETTARLQEKYGTDSAVAAIGP
                     AGEMLLTAAAITNADLHGLASRINGRGGLGAVMGAKRLKAIVIENASPPKAAADSGRF
                     KAAVNEFEELLKGNSRKNAPLAETALQQNCLPSGSAAVNGAQAKLGAPVKPETARAFR
                     QYCEITDWTAISELNETANDLGLDTLETAAALAVLMQAEMIPWGDGKAALQMIKEVRE
                     GTSLGYILGQGGSFAAEAFNVQLSEDKVVSKAAASGSAARDKQFNVCIAAALQNEQEF
                     PGRRQGELQDELGADMPLLTNHLLDTLGVCLYAAPAFTRQPTAWSTMAELLNARYGWE
                     QTAESLLALSRDALLKEREFDAAAGYLGESNRLGGYLPLSRQDHHTHSAVMWQQQSGS
                     QPFIQ"
     gene            complement(2229..3284)
                     /locus_tag="VFC74_06825"
     CDS             complement(2229..3284)
                     /locus_tag="VFC74_06825"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HZK25085.1"
                     /translation="MYNIAVLAGDGTGPEVIREGLKVLDAISENQGLKLNYTHYDLGG
                     TRYLRTGEVLPDSVLAELRQFDAIYLGAIGHPEVKPGILEKGLLLRLRTELDLYINLR
                     PIKLFPGVETPLKDKGPQDIDFVVVRENTEGQYVGAGGIMRKGTPHEIAIQESVCTRM
                     GVERCLRYAFEYAQRRNQGNKVTLCGKTNVLTHVFDLWERVFHEVGQQDYPDITRDYA
                     HVDATVMWMVKNPEWFDVIVTENMFGDIITDLGAMIQGGMGIAAGGNINPEGVSMFEP
                     IGGSAPKYTGQNVVNPLAAIGAAAMMLDTLGETAAAAKIEKSIMHVCEKDIKSQAAGK
                     MGHTTQAVGDLVVKNLS"
     gene            3468..6902
                     /gene="addB"
                     /locus_tag="VFC74_06830"
     CDS             3468..6902
                     /gene="addB"
                     /locus_tag="VFC74_06830"
                     /inference="COORDINATES: protein motif:HMM:TIGR02773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HZK25086.1"
                     /translation="MSIRFILGRAGSGKTFTCLQAIAEASKTEPLGAPLILLVPEQAS
                     LEMEKELAVMCGGGTFRAQVLSFRRLAYRLLQASGSQLPVMTELGRQLLLRRLLQDET
                     SSLSAFARAARQPHFCQQLAVQLREFKHYQITTDNLAALAQSVDCPTALRGKLADLAA
                     IYTAYHAATAGSYTDSEDTLTQLAAAIAAGGLPQQTKVWVDGFAGFTPQEFSVLGALM
                     GAVSHLEIALCLDPHTKKQPQEEDLFHPTQDTYWRLRRLSGETAVKVLPPLELPQANQ
                     TTRFSAAAPLAHLEANFAVFPLIPYQQQTSCLQLVTTAGPRAEVEAAARDIVQRVREE
                     GWRYRDIGIILRNFSTYHDLVAAIFLDFDIPYYVDERRSAAHHPLVEFLRSAVDTVSS
                     NFSCQPIMQLLKTDLFPLRRQDVDQLENYVLAHGIYGSRWLTDKPWNYHHQLTLAEER
                     AEQTTSEDTSKLVDDTRRQFIKIFGPFAQAVGGGEKQVVAVYCQALWKLLEQVEAAQT
                     LQNWAETASADGQLSQALEHRQVWQGVVELLEQLVGILGNQQLTPPEFAQVLGTGLEN
                     LKLGLVPAMTDRVVIGNVERSRQPRLLAAYVLGLSEGDFPARLQEEGLFADEEREALG
                     DNGLELAVTRRQRLFHEQYLSYIALTRSSDYLWVSCPLADDEGKAKRPSALFNHLREF
                     FPVNEVQFYGNTPDPQEDYQAVTKPHRIAAALLQQAGTALRGGAISPFWAAVYNEALA
                     HAPTSTKLQQLWPALNYQNHIPSLQPATVRALFGTKLHSSVSRLEQFAQCPFAHFARY
                     GLRLKERADFSLEAPDMGMFFHAALRIFVTQLLEEKLAWSALTIEEAVVRINEIVDDL
                     VPQLHREILLSSARLRFLAARLKETLAQAITALTIHAQQSVFQPVAVELPFGGGELPA
                     WQLQYGQQEIMLHGQIDRLDVAEANGQVYYRVIDYKSTPTSLKLSELWYGLSLQLLTY
                     LAVVNENKANFSQLAGKGVGALYFGIYRPFTRVANPGAAAEEAVPKLDGLILADQQVF
                     KLMGGEKLVHAALKKDGNFTKVSRVAEETQLQQLLQYVCEQIGESAVRILQGEVTIKP
                     FKKPDGRKACTYCPFMPLCCFDVTVEGNQYRLISPLAAEEVLQAVAAKQEGASQDE"
     gene            6895..10587
                     /gene="addA"
                     /locus_tag="VFC74_06835"
     CDS             6895..10587
                     /gene="addA"
                     /locus_tag="VFC74_06835"
                     /inference="COORDINATES: protein motif:HMM:TIGR02785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HZK25087.1"
                     /translation="MSKQWTAEQLAAIETGGCNLLVAAAAGSGKTAVLVERIIRLLCR
                     SEAPLDIDKLLVVTFTEAAAAEMRERIAAALEREISTARRLDLVKQLSLLNGASISTL
                     HSFCLEVIQRYFYLLELDPSFRVADELEASMLRQEIVALVLEKAFAEEDEDFLALARH
                     YGGRTSDEGLVSLILQLYRFCWSNPWPERWLDEAVNLYALNAEADPESALGVWLNPLR
                     EELHLTLEQAADTLHEALALCQLPQGPLVYEAALQKELEQVKSLLTLLGGPWSELREA
                     WLAVTFDRLRAAKDVDEKLKTRVKALRDGAKDLLKKSSITYFTRSLSEYVSEITALAP
                     LLSSLVKQVKEFAHAYATAKKQAGLMDFNDLEHYCLQILLANEADAASLQPSAAALEL
                     RARYAQVLVDEYQDINPVQDAILQLVSRQGEEAPNLFMVGDVKQSIYRFRLGDPGLFL
                     DKYNRYAVNNDKVDSTERKILLSRNFRCRTNVVATVNYLFSQLMTAQAMEIEYDQEAE
                     LVCGATYPVAADIKLIEEAVEVFLLEKDIAANSLASAEADAEEEDLTSLEREGVVVAQ
                     QIQQLVRKQGSATHIFDKDNGCYRPVSYRDIVILLRSTSNRASRLAAILARYNIPAYA
                     ELTTGYFAATEVETMLSLLQVLNNPCQDIPLAAVLRSPFVSFSAEQLAAIRQVGGRNC
                     DFWQALLTAAQAELPELSLRCQAFLQQLDRWRDLARREKLGTLLATIYRESGYADYVA
                     GMPDGMKRQANLRALFSRARQFDRFSRQGLSRFLQFIEQLRKSGEDLGAARALGENED
                     VVRIMSIHKAKGLEFPIVFLSDLGKSFNFQDQRAELLLHRHLGLAPLIVEPEAKLRYP
                     SLPYLALRLVGEAETRAEELRILYVALTRAREKLYLVGSVKKLTETMDTWGELVSCPT
                     KQLPSYHVRRARTYLDWLGKALSRHPQIKAANSARGWQPAIEAHLKLTCVEQVEADFD
                     LASQAQEADCKQALLTLSPLPVETPVDVKKAIKERLCYQYPYPPSSVPAKLSVTEIKR
                     RFAQAEEEEGEAHRLITSPWPRPNFIQKQGLTPVERGLLYHSLLQHLDLTQPLDEAGI
                     VEQIRQLVQAGILAEMFLSYLDPAKVTAFFRSEAGQLMLTYKEQVLREWPFTYALPAA
                     ELAAEEHETVLIQGIIDVLVSTPEGFVIIDYKTDRLPTEGMAELAKRYQVQLAYYARA
                     VETILHEPVQAAYLYAFAADRAVRVL"
     regulatory      10666..10843
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            10914..11969
                     /gene="cobT"
                     /locus_tag="VFC74_06840"
     CDS             10914..11969
                     /gene="cobT"
                     /locus_tag="VFC74_06840"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008939 -
                     nicotinate-nucleotide-dimethylbenzimidazole
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HZK25088.1"
                     /translation="MEKLQSTLNRIVPTDKNKEEEAQRYIDGLTKPVGSLGVLEDIAR
                     QMAGITGQVKPQIPKKTSILMAGDHGVVAEGVSAYPQEVTPQMVLNFLSGGAAMNVLA
                     RHANAELIVVDMGVAVDLPEHPILLNRKVAYGTANMAQGPAMTREQAIAALEAGIEVA
                     ESYIATGTGLFGTGEMGIGNTTPSAAITAFYKGCSVAEVCGRGTGLDDQGLQLKIKVI
                     EQAIAVNKPDPDDALDVLAKLGGYEIAGMAGVMLAAAANRVPVMVDGFISAVSAIIAS
                     RLHPHAVEYMLASHLSEEPGHITALSLLELTPFLRMNMRLGEGTGTALAMSMVDAALK
                     IVHEMASFADAGISGAL"
     gene            12453..13121
                     /locus_tag="VFC74_06845"
     CDS             12453..13121
                     /locus_tag="VFC74_06845"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HZK25089.1"
                     /translation="MIPLRDTPKTRKFPYVNTLLIIFNIFVFVQQLALTNEQLANVVV
                     RYGLIPLRFSQSIAAGEAAAALAPLVTYQFMHGGWLHIGSNMLYLWVFGDNIEDRVGH
                     LRYLIFYLLMGLLAGLLQVFLDAASPIPIIGASGAVAGILGAYLVSCPRARVLAIIPI
                     FILFTITEVPAILFLGFWFILQLFSGFTSIGVNVSIAWWAHIGGFVAGMALIRFFGER
                     IKCE"
     gene            complement(13160..13639)
                     /locus_tag="VFC74_06850"
     CDS             complement(13160..13639)
                     /locus_tag="VFC74_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HZK25090.1"
                     /translation="MDYLPLILASLLFIVGVLGTVLPVLPGAILIFGGMLLYGYLTNF
                     AVLTINFYLLQGVAMLLVFLIDFLATAVGTKRYGGSRVASFGAAIGTLVGIFSFPPLG
                     IVVGPFLGAVLAELLVGKDTNQALRVGFGTLIGLLGGTILKLVIEAAMIIYFFLTIF"
     gene            13790..15487
                     /gene="glgP"
                     /locus_tag="VFC74_06855"
     CDS             13790..15487
                     /gene="glgP"
                     /locus_tag="VFC74_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010964175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HZK25091.1"
                     /translation="MPNQSKLPRVAYFCMEYGLNSGLPIYAGGLGILAGDFLKAAGDL
                     HLPMIGIGILWRQDYTEQFIGEDGWPYDMYPNYDFEFVKDTGITVNVEVEGQQVPCKV
                     RLVDQYGNAPLYLLDTNFPGSEYGWITNKLYGGGSTERITQEIVLGIGGVRLLRALNI
                     DVDVYHFNEGHAVLAGIELIRENMAEGMTFEDALRAARRQVVFTTHTPVEAGNEKHNH
                     GELEKLGAYNGLSYEQMARLGGDPFNMTEAALRLCAVANAVSNLHGHTARAMWRHVAD
                     TGPIISITNGVHVPTWQAPEIHRAFKSGADLWKPHQQAKQELINFAEKHTDATLNPNA
                     LIVGFARRAAGYKRSDLIFRNTEVIDPLLRAGKMQLIFSGKAHPNDNEGKRIIRDLVA
                     MDRKYKDSVVFLENYNMEIARLLIRGCDVWLNNPVRPLEASGTSGMKAAMNGVLNLSV
                     VDGWVGEGPQHGISGWLLDAHHSHIDPQQQNMDDLHALYKVLFDEVIPTYYNDRSRWI
                     DMMRASIDMSHFKFSATRMVREYYELMYRQVTDKQDAITAPWMVPQRSHGEQHPYIT"
     gene            15731..16399
                     /locus_tag="VFC74_06860"
     CDS             15731..16399
                     /locus_tag="VFC74_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25092.1"
                     /translation="MSEDFYVQDNLKPTEAELVFNSTTSKKNLSGVRGRASRTGRTGA
                     IRTPVDFLSGKAKKEYMKSSEVKVYNMYEDQVLPYDEFKELPIEKQVVLMTRWRDEIG
                     TNNIIRDMKISRKKFYSEVLKTLGIETKPRGRRPKKIETDEYKTEQTEIIEKTKAEVS
                     AEIVPETPAKVILAPEQQETSFNIVFNGRFAGHQLSNRLARLARVLDAEEQYEIKVEI
                     RELI"
BASE COUNT         4422 a         3576 c         4114 g         4406 t
ORIGIN      
        1 catcacgata ttaaacactg tacatcccta cctttccata atttgatcat aacgttgaag
       61 cagtttctca atcatttgta cccgtttagt taaagttttg atccagctag tcagaaaacc
      121 ttcgccttgt gcggttaatt gatagcgtct gcgcgccggt cctgctttgc ctgtttccca
      181 atttgaaacc acaaaaccat gtttctccat gcgccgcaga gtgcgataga tttgccctgg
      241 atccacgttg ggctcaaaat caaattgaga caaaggttca atcagctcat aaccatgcgc
      301 tggcttttcg gcaagcaaca acaacaagca tggttctatg aagcgttcaa tcttgccttt
      361 atatggcagt aagtcgtgtt gtttttctgc tttctgattc ggcgccaata ggttcacctc
      421 acatctatgt gtaaacttca tatacctatt ataaaagtga gctctgctga ttgcaagtgt
      481 aaattttgca agtaccttac acttttgcgc ctgcttacat agctttcagg taaagggata
      541 ctctaaaaaa tatgaataat atgtagaagg cggtgtaaga catgacttat ttgctgcgcg
      601 ttaatatgtc cacgcaagcg gttaccacca gtgagctacc accagattat caacggttag
      661 gtggacgcgc cctaacctca aggatttgcc aggatgaagt agacccattt tgctatgccc
      721 tgggtgaaag aaataaacta gttattgctc cagggctatt agcggctgcg gattttccat
      781 atggcggacg tattgccatg gcagggaaaa gcccgctgac gcagggatta acagcgtcta
      841 atgctggtgg tctaacagcg gctcgactgg gagctttagg cattcaagcc atcgttgtag
      901 aaggcttgcc ccaagatgac aaactttacg tgttagttat tagtgaaaag gaagcaaggc
      961 ttgtaccgac gccagaactg cgcggtaaag gtacttccga gaccacagcc aggctgcagg
     1021 agaagtatgg cactgatagt gccgtggcag caattggtcc tgccggagag atgctgctga
     1081 cggctgcagc tatcactaac gctgatcttc atggtttggc cagtcgtatt aatggtcgtg
     1141 gtggtttagg tgcagtaatg ggagcgaaac gtttaaaagc tattgtgatc gaaaatgcct
     1201 cgccgccaaa ggctgctgct gacagtggac gttttaaggc tgcagtaaat gagttcgagg
     1261 agctccttaa aggaaacagc agaaaaaatg ccccactggc agagacagca ctgcagcaaa
     1321 attgcctgcc ttctggcagt gcagcagtca acggagcgca agctaaatta ggggcacccg
     1381 ttaaacctga aactgcgcgt gcttttcgcc agtattgtga aattactgat tggacagcca
     1441 ttagtgagct aaatgagaca gccaatgact tggggttaga tactctagag acggcagcag
     1501 cactggcggt gttgatgcag gcagaaatga ttccttgggg agacggtaag gccgcgctgc
     1561 aaatgataaa agaggtgaga gaaggtacct cactgggtta tatcctaggt cagggcggca
     1621 gctttgccgc tgaggctttt aatgtacaac tttctgaaga caaagtggtg agcaaagcag
     1681 ctgccagtgg cagcgcagcg cgagataagc agtttaacgt ttgtattgcc gccgctttac
     1741 aaaatgagca agagtttcct ggacgccggc agggggagct gcaagacgag ttgggcgctg
     1801 acatgccttt gctgactaac cacttgcttg atactctggg agtttgtctc tacgcggcac
     1861 ccgccttcac tcgccaacca actgcctggt ccacgatggc ggaactatta aatgcgcgct
     1921 atggctggga acagacggcg gagtcactgt tagccttaag ccgtgatgct ctgttaaagg
     1981 aacgcgagtt cgatgcagct gccggctacc ttggtgaaag taaccgtctt ggtggttatt
     2041 taccattgtc acggcaggac caccatactc attctgcggt aatgtggcag cagcagtccg
     2101 gctctcagcc ttttatacag taagagaaaa gggattagga aagaaagcga tcaagttgga
     2161 gataatttac tggcagccga taaaaaaagc ttactcttcg aaattgagga gtaagctttt
     2221 tacttaaatc atgacagatt tttaactacc aggtcaccta ctgcttgtgt agtgtgtccc
     2281 attttcccgg cggcctgact ttttatatct ttttcacaaa catgcataat cgatttttct
     2341 atcttggcag cagctgctgt ttcgcccaga gtgtcgagca tcatggccgc ggcaccaatg
     2401 gccgctaagg ggttaacaac attttgaccg gtatacttgg gtgcagaacc gccgatcggc
     2461 tcaaacatgg agacgccttc agggttaata ttaccaccgg cggcaatgcc catcccgcct
     2521 tgaatcatgg cacccaggtc cgtaatgata tcgccaaaca tattttctgt gacaatcaca
     2581 tcaaaccatt cgggattttt taccatccac atcactgtgg catccacatg agcgtaatcg
     2641 cgagtaatgt cggggtagtc ttgctggccg acttcatgga aaacccgctc ccaaagatca
     2701 aagacatgcg tcaggacatt agttttaccg cacagtgtga ccttgttgcc ttggttgcgc
     2761 cgttgtgcgt attcaaaggc ataacggaga cagcgctcta cgcccatccg tgtacacact
     2821 gattcttgaa tggcgatttc gtgtggtgtc cctttgcgca taatcccacc ggctcctaca
     2881 tattgccctt cagtattctc acgtactacc acaaagtcaa tatcctgcgg tcccttatct
     2941 ttgaggggtg tttctactcc ggggaagagc ttaatgggcc gtaaattaat ataaaggtct
     3001 agttcagtgc gtaaacgtaa cagcaagccc ttttctaaaa tccctggttt gacttcagga
     3061 tgaccaatgg ctcccagata aatcgcatca aactgccgca attcagccag cacactatcg
     3121 ggcagcactt cacctgtacg caggtaacgt gtgccgccta aatcgtaatg cgtataattt
     3181 agctttagac cctggttttc actaatcgcg tccagaactt ttagaccttc acggataacc
     3241 tctggacctg taccatcgcc ggcaaggacg gcaatattat acatcggctt tcctccattc
     3301 ctatgtaaaa gtattgtgtt taattttatt acatcctgca gaaaaaggaa agagcaagat
     3361 agcaggtaaa caggaataat tacggtgcat cacgaattca aagaggagaa gcaagtaagt
     3421 aactgtttag taactgcaca attagcgaag aaacagggga gatgtttatg tcaattcgct
     3481 ttattctagg tcgcgctggt agtggcaaaa cttttacctg cctgcaagca atcgctgaag
     3541 caagtaaaac tgagccgctg ggggcgcctt taatattatt agtgcctgaa caagcctcat
     3601 tggaaatgga gaaagagctg gctgtcatgt gtggcggcgg cacttttcga gcgcaggtac
     3661 tgtcctttcg tcgtttagcc taccgcctac tgcaggcaag cggcagccaa ctgccggtga
     3721 tgacggaatt aggccggcag ttattgttac gtcgcctact gcaagacgag acgtcaagtt
     3781 tatcggcttt tgcccgcgcc gcccggcagc cgcatttttg tcagcagctg gctgtgcagc
     3841 tgcgagaatt taaacattac caaattacaa cagacaattt agcagctctg gcgcaaagtg
     3901 tggactgtcc aactgcactg cgcggtaaat tagctgatct tgctgctatt tacacagcat
     3961 atcatgcggc aactgctggc agctatactg attctgaaga tactttaaca cagctggctg
     4021 ctgctatcgc tgcaggtggt ctgccccagc agactaaagt atgggtagat ggttttgccg
     4081 gttttacccc gcaggaattc tcggtgttag gcgctttaat gggtgccgtg agccatctgg
     4141 aaattgccct gtgcctcgat ccccacacaa aaaaacagcc tcaggaggaa gatttattcc
     4201 atcctacaca ggatacatat tggcgcctgc gccggttgag tggggaaaca gctgttaaag
     4261 tattaccgcc cttggaactg ccgcaagcta accaaaccac acgctttagt gctgccgcgc
     4321 cactagccca tttagaagca aattttgctg ttttccccct gattccttac cagcagcaga
     4381 cttcatgcct tcaactagta accacggccg gacctcgggc cgaagtggag gcggcagcac
     4441 gtgatattgt gcagcgagta cgggaagaag gttggcgcta ccgggatatt ggcattatct
     4501 tacgtaattt ttcaacttat catgacttgg tggctgctat atttttagac tttgatattc
     4561 catactatgt tgatgagagg cgttcggccg ctcaccatcc actagtggag tttttacgtt
     4621 ctgctgtaga tacagtaagc agcaatttta gctgccaacc aattatgcag ctattaaaaa
     4681 ctgatttgtt tccactccgc agacaagatg ttgatcaact ggaaaactat gttttagcac
     4741 atggcattta cggcagccgc tggttgacgg ataaaccctg gaactatcac caccaattaa
     4801 ccctggccga agagagagca gaacagacaa caagtgagga tacaagtaag ctggttgatg
     4861 atacccgccg tcagttcata aaaatctttg gaccatttgc ccaggccgta ggtggaggcg
     4921 aaaaacaagt ggttgcggtc tactgtcagg cgttatggaa gctgttggag caagtagaag
     4981 cagcgcaaac gctgcaaaac tgggctgaaa ccgcttcggc tgacggccag ctctcacaag
     5041 cactggaaca tcggcaggtt tggcagggag ttgtggaatt attagaacag ctggtaggga
     5101 ttttgggtaa tcaacaactt accccgcctg aatttgcgca agttttaggg acaggcttgg
     5161 agaacttaaa gttaggttta gtacccgcca tgactgaccg tgtggtgatt ggcaacgtgg
     5221 aacgctcacg acaaccacga cttttagccg cctatgtttt gggtttaagt gaaggtgact
     5281 tcccggctcg tttacaggag gaaggcttat tcgccgatga agaacgggaa gctctcggtg
     5341 acaatggctt ggaattagcg gttacacgcc gccagcgttt atttcatgaa cagtacttgt
     5401 cctatattgc cctaacacgc agttcagatt acttatgggt tagctgtcct ttggctgatg
     5461 atgaaggtaa agcaaaacgt ccttctgcgc ttttcaatca tctgcgcgaa ttttttcctg
     5521 tcaatgaggt gcagttttat ggtaatacgc ctgaccccca agaagattac caagcggtga
     5581 caaaaccgca cagaattgct gcagctctct tacagcaggc agggacagcc ttaagagggg
     5641 gcgctatttc acccttctgg gctgctgtct acaatgaagc acttgcacat gcccccacat
     5701 caactaaact gcagcaactt tggcctgctc taaactatca aaaccacatt ccgtctctgc
     5761 agccagccac agtgcgggcg ctttttggaa ctaaactgca tagcagtgtg tcacgtttag
     5821 agcagtttgc ccagtgccct tttgcccatt ttgcccgcta cggactgcgt ttaaaggaac
     5881 gggcggattt tagtcttgaa gctccagaca tggggatgtt ttttcatgcc gccttacgaa
     5941 tttttgtgac acagctgctg gaagaaaaac tagcatggtc tgctttaacg atagaagagg
     6001 ctgttgtccg catcaatgag atagtcgatg acttggtacc gcagctgcac cgggaaatat
     6061 tgctttccag cgcccgtttg cgcttcttgg cagcccgctt aaaagaaact ctggctcagg
     6121 caataacggc tttaactatt catgctcagc agagtgtctt tcaaccagta gcagtggaac
     6181 tgccttttgg cggcggtgaa ctgccggcct ggcagctgca gtatggtcaa caggaaatta
     6241 tgctgcacgg gcagatagac cgccttgatg tggctgaagc taacggtcag gtttactatc
     6301 gtgtgattga ttataaaagc actccgacct cgctgaaact tagtgagctt tggtatgggc
     6361 tctcattaca gttgttgact tatctggcag tagtaaatga aaacaaagcc aattttagtc
     6421 agctcgcagg caaaggcgtg ggcgctttat attttggcat ctatcgtccc tttactcgtg
     6481 tggcaaatcc aggggcggca gcagaagagg ccgtgcctaa actggatggt ttgatcctcg
     6541 cagaccagca ggtctttaag ctaatgggtg gggaaaagtt ggtgcatgcg gccttaaaaa
     6601 aggatgggaa ttttactaag gtctcccgtg tagcagagga aacccaactg cagcagctgc
     6661 tgcagtatgt ctgtgagcaa atcggtgagt ctgctgttcg tatcctgcaa ggtgaagtta
     6721 caattaaacc ttttaaaaaa cctgatggca gaaaggcctg cacttattgt ccctttatgc
     6781 cgctttgttg ttttgatgtc actgtggagg gaaaccaata ccggctgata tcaccgcttg
     6841 ctgcagaaga ggtcctccaa gcggtggcag ccaagcagga aggagcgagt caagatgagt
     6901 aaacaatgga cagcagaaca acttgcagcc attgaaacag gcgggtgtaa tctactcgtt
     6961 gccgcagctg ccggttcagg aaaaactgcg gtgttggtgg agcggattat ccgtctgtta
     7021 tgtcgttcgg aagctccttt agatatagac aaattattag tcgtgacttt tacagaagcg
     7081 gcggcagcag aaatgcgcga acgtattgct gctgccctgg aacgagaaat ttcaacagca
     7141 cggcgtctgg atttagttaa gcagctctca ctcttaaacg gcgcctccat atctacactg
     7201 cattcctttt gtctagaggt tattcagcgt tatttttatc tgctagaact agatccttct
     7261 tttcgtgttg ccgacgaact tgaagccagc atgctgcgcc aggaaattgt ggcacttgtt
     7321 ttagaaaagg cctttgctga ggaagatgaa gattttctgg ctctggctag acactacggc
     7381 ggcagaacta gtgatgaggg tttggtcagt ttaattctgc agctttatcg tttttgttgg
     7441 agtaatccct ggcccgagcg ctggctggat gaggccgtta atctctatgc gctgaatgcg
     7501 gaggcagacc cagaaagtgc cctgggggtg tggttaaacc cgttacgtga agaattgcat
     7561 ctgaccttgg agcaggcagc agatactttg catgaagcgt tggcactttg tcagctgccg
     7621 cagggaccct tagtttatga ggcagcttta cagaaagagc tggagcaggt caaaagtttg
     7681 ctaaccctct tgggggggcc gtggtcagag ttacgtgaag cctggttggc agtgactttt
     7741 gaccgcttgc gtgccgcaaa agatgtagat gagaagctta aaacgcgcgt taaagccctg
     7801 cgcgatgggg ccaaggattt actaaagaag agttctatca cttactttac gcgttctttg
     7861 tcggaatatg tcagcgaaat aactgccttg gctcctttgc tgtcttccct ggtaaaacag
     7921 gttaaggagt tcgctcatgc ttatgccaca gctaaaaaac aagcagggct gatggatttt
     7981 aatgatctgg agcattattg tctgcagatt ttactggcaa atgaggcaga tgcagctagc
     8041 ctgcagcctt cggccgccgc cctcgaacta agggctcgct atgcacaagt gttagtagat
     8101 gagtatcaag atatcaatcc cgtgcaggat gccattttac aactagtctc tcgccaagga
     8161 gaggaagcac ccaatctgtt tatggtcggc gatgtgaagc aaagtattta tcgttttcgt
     8221 cttggtgacc ctggtctctt tttggataaa tacaatcgct atgctgtaaa taatgacaaa
     8281 gtggacagta cagaaagaaa aattctttta agccgcaatt tccgctgccg gactaatgtg
     8341 gtggccacag taaactatct atttagtcaa ctgatgacgg cgcaagccat ggaaatcgaa
     8401 tatgatcaag aagcggagtt agtctgtggc gcaacctatc ctgtggccgc agatataaag
     8461 ctgattgagg aagcagtgga agtttttcta ttggaaaaag acattgccgc aaattcgctc
     8521 gcatctgcag aggcagacgc ggaggaagaa gacttgacaa gcttggagcg ggaaggcgtg
     8581 gtggtggcac agcaaattca gcagctagtc agaaagcaag gcagcgccac gcatattttt
     8641 gataaagata atggctgcta tcgtcccgtc agttatcgtg atattgtcat tctactgcgt
     8701 tctacatcta atcgtgctag caggttggcc gcaatattag ctcgctacaa cattcccgcc
     8761 tatgctgaac ttactaccgg ttactttgcc gctacagaag tagaaactat gctttctttg
     8821 cttcaagtgc taaataaccc ttgtcaagac ataccattag ctgctgtact gcggtctcct
     8881 tttgtcagct ttagcgcgga acagctagct gccattcgcc aggtcggcgg ccggaactgt
     8941 gatttttggc aggccttact tactgcggcc caggcggaac tgcccgagct ctcgctcagg
     9001 tgccaagctt ttttgcagca gcttgaccgc tggcgcgatt tagcaagacg tgaaaaatta
     9061 ggcacgctgc ttgcgacaat atatcgtgaa agtggttatg cagattatgt ggctgggatg
     9121 ccagatggta tgaaacggca agcaaatttg cgggcccttt tttcccgcgc ccggcagttt
     9181 gatcgttttt ctcggcaggg actgtcacgt tttctgcaat ttatcgaaca actgcgtaaa
     9241 tctggtgaag atttaggcgc tgcacgtgcc ttgggtgaaa acgaagatgt ggtacggatt
     9301 atgagcattc ataaagccaa gggattggaa tttcctatcg ttttcttgtc tgatttaggc
     9361 aaatctttta acttccagga tcaacgcgcc gaattactgc tgcatcgtca cctggggtta
     9421 gcaccgctga ttgtggaacc agaagcgaaa cttcgttatc cctccttacc atatttagcg
     9481 ttacgtttgg tgggggaagc agaaacacgc gctgaagagc tgcggattct ttatgtcgcc
     9541 ctcacacgag caagggaaaa actctattta gtaggttcag ttaaaaagct gactgagacg
     9601 atggacacgt ggggagaact agtaagttgc cccactaaac aattgcccag ctatcacgta
     9661 cgtcgggcca gaacatacct tgactggctc ggcaaagcgt taagtcgtca tccgcaaatc
     9721 aaggcggcaa acagtgctcg tggctggcag ccagcaatag aagcgcacct taagctgacc
     9781 tgtgtggaac aggtagaagc tgattttgac ttggcgtcac aagcgcagga agctgattgc
     9841 aaacaggcgc tcttaacttt gtcgccgctg ccagttgaga cgcccgtaga cgttaaaaag
     9901 gcgattaagg aaaggctttg ctatcagtac ccttatccac cctccagtgt gccggcaaaa
     9961 ctatcagtaa ccgaaataaa gcgtcgtttt gcgcaggctg aagaagagga gggagaggcc
    10021 cacagactta tcacatcacc ttggccgcgt cctaacttta tccaaaagca gggcctgacg
    10081 cctgtggagc gcggcttact ttaccacagt ttactgcagc atttagactt aactcaacct
    10141 ctggatgaag ctggaattgt ggagcaaata cgacagctgg tccaagcagg aatcctggca
    10201 gaaatgtttt tgagctattt ggatccagca aaagtaactg ctttctttcg ttcagaagca
    10261 ggccagctga tgcttactta taaagagcag gttttgcgcg aatggccttt tacctatgcg
    10321 ctgccggcag cagagctagc cgcagaagag catgagacag tgctcattca gggaattatt
    10381 gatgtcttgg tgagtacacc agaagggttt gtaattattg actataaaac tgatcgctta
    10441 ccgacagagg gcatggccga actggcaaag cgctaccagg tgcagcttgc ttattatgcc
    10501 cgggctgtgg aaactatttt gcatgaaccg gtgcaggccg cctatcttta tgcttttgct
    10561 gctgatcgag cagtgcgtgt gctgtaagca gccagttagt ttaattagtt aaacctgctc
    10621 gcaagctatt gacaaggata aacgagcttt gtataataga gataaattaa atagatggtt
    10681 tgtggtgctg cagatgcgga gaatagggaa tcgggtgtaa atcccgaacg gtcccaccac
    10741 tgtaaccggg gagaaactgc caagaccact gtccgtaacg acgggaaggt ggcagggaca
    10801 aagagccgga gtcaggagac ctgccacaaa gctgtgtaat attggatgat ggtaaagtcc
    10861 tcaagctttc gcttgaggac tttttcttat ttaaaatcaa aggaggagca aaaatggaaa
    10921 aattacaaag cactttaaac cggattgtac caactgataa aaacaaggag gaggaagctc
    10981 aaaggtatat tgatggtctc actaaaccag taggcagttt aggggtttta gaagatattg
    11041 ccaggcaaat ggcaggtatc acaggtcaag ttaaaccaca aatacctaaa aagacttcca
    11101 tcctaatggc cggggaccat ggtgtggtag cagaaggtgt ttctgcttat ccgcaagaag
    11161 ttacaccgca gatggttcta aacttcctca gcggcggtgc agccatgaat gtactggctc
    11221 gccatgccaa tgcagaacta attgttgtcg atatgggggt ggcagtcgat ctgccggagc
    11281 accccatctt acttaaccgc aaggtagctt atggtacagc caatatggca caagggccgg
    11341 ctatgacacg cgagcaagct atcgcagcct tggaagcagg cattgaagta gctgaatcat
    11401 atattgctac tggtaccggt ctgtttggta caggtgaaat gggtattggc aacaccacac
    11461 ccagcgcagc tatcactgca ttttacaaag gctgctccgt agcagaagtc tgtggtcgtg
    11521 gtacaggttt agacgatcaa ggtctgcagc taaaaattaa agtaattgaa caggcgattg
    11581 ccgtgaataa gcctgacccg gacgatgccc tagacgtctt agctaaactg ggcggttatg
    11641 aaatagcggg catggctggt gtaatgctcg cggcagccgc aaatcgcgta ccagttatgg
    11701 tagacggttt tatctccgct gtatccgcca ttattgcttc tcgcctgcat ccgcatgctg
    11761 tagaatacat gttagcatcg catttatcag aagaaccagg ccatatcaca gccttaagct
    11821 tactagagct gactcccttc ttacgcatga atatgcgctt gggtgaaggt acaggcacag
    11881 ccttagccat gtctatggtc gacgccgcat taaaaattgt ccatgaaatg gccagttttg
    11941 ctgatgccgg tatctccggg gctctttaaa gctaattaaa taaattatgt ttaatgtacg
    12001 ggaaacatcg ttttcccgca catttttttt tttgtttttg gcatacggta agaagaaggt
    12061 ttaattctga acaagttgtt aaatattatt aaagcaagaa cctcgtacgc aggaaaatac
    12121 cgccagcagt aagaagaaat actaaactaa agtaaaggcg gtaactgcag atgagcacaa
    12181 ctacaaaaat agttatttta cttaatctga tcactctgct gttaatgttt tacgataaaa
    12241 ggcaagctca acgggcacaa tggcgggtgt cagagaaaac gttgctgttt ttagccatgc
    12301 tcggaggcag tgtcggcgtt tggttgggga tgaaaagttt tcggcataaa actaaacata
    12361 agctttttta ctggggcctt cccctgctcc ttagtttgca agcggcattg ttattatact
    12421 tttataggca aaattggtag gaggcgaatg gcttgattcc tttaagggat acaccgaaaa
    12481 caagaaaatt tccctacgtc aataccttgc tgatcatctt caatattttt gtgtttgtgc
    12541 agcagttagc tctaactaac gagcagctag ccaatgttgt ggtgcgttat ggtttaatcc
    12601 ccctgcgctt tagtcaaagt atcgctgcgg gggaggcagc agcagctctg gctccacttg
    12661 ttacttatca atttatgcat ggcggttggc ttcatatcgg tagtaatatg ttgtatttgt
    12721 gggtctttgg cgacaacata gaagaccgcg tcggacatct caggtatctt atcttttatt
    12781 tgttaatggg gctcttggct ggcttattac aggtttttct cgatgccgcg agtccaatac
    12841 caattatcgg tgccagtggt gccgttgcgg gcattcttgg ggcttatctt gttagttgcc
    12901 cgcgggcacg ggtgttggca atcatcccta tctttattct ctttacgatt actgaagtgc
    12961 cggcgatttt atttctcggt ttttggttta ttctacagtt gttctccggt ttcacaagta
    13021 tcggcgttaa cgtttctatt gcctggtggg ctcatattgg cggctttgta gcaggtatgg
    13081 ctttaatccg gttttttggc gagcgcatta aatgtgaata atagtaataa aaacccgcct
    13141 gtggcgggtt tttttgttat taaaagatag tgagaaagaa ataaataatc atagctgctt
    13201 caatgactaa tttgaggatg gtgccgccga gcaaaccgat cagtgtgcca aagccaacgc
    13261 gtaaagcctg gttagtatct ttgccgacaa gtaattccgc cagtactgca cccaaaaagg
    13321 gaccaacaac aatgcctaaa ggagggaagg agaaaattcc tacgagcgta ccgatggctg
    13381 cgccaaagga ggcgacgcgg ctgccgccat agcgttttgt gccaactgca gttgctaaaa
    13441 aatctattaa aaatactaac agcatagcta caccctgtaa aagataaaag ttgattgtta
    13501 atacggcaaa attggtcagg taaccataca gcagcatgcc gccaaaaatt aaaattgctc
    13561 caggcaaaac aggtaaaaca gtacctagaa ctcccacgat gaataataaa cttgccagga
    13621 ttaatggtag atagtccatt aagttctctc ctctgaaaaa aatttatatt aatttatatt
    13681 cgggcttagt gatttggttc ctgcgtttta gtatattctt tcgaatgctt caacaaaata
    13741 aacaagaagt tcaaagaaag gatggtgaag accggctttg ccggcaagaa tgcctaatca
    13801 aagtaaacta cccagagttg cttacttttg tatggaatat ggtcttaact caggactgcc
    13861 aatttatgca ggcgggttgg gaattttggc tggtgatttc ttaaaagctg ctggcgactt
    13921 acatttacca atgatcggta ttggtatctt atggcgccaa gactacacag aacagttcat
    13981 tggagaagat ggctggcctt atgacatgta tcctaattat gattttgagt ttgtgaagga
    14041 tacagggatt accgttaatg tggaggtgga aggacagcag gtaccatgta aagttcgttt
    14101 ggttgatcaa tacggtaatg ccccgctata tttattagat acaaactttc ccggcagcga
    14161 gtatgggtgg ataaccaata aactatatgg tgggggttct actgaacgga ttacccagga
    14221 aatagtactt ggtattggtg gtgtccgttt attacgtgct ttaaatatcg atgtagatgt
    14281 atatcacttt aatgaaggtc atgccgttct cgccggtatt gaacttattc gtgaaaatat
    14341 ggcggaggga atgacttttg aagatgccct gcgggcagca agacgtcaag tagtttttac
    14401 aactcatacc cccgttgaag ctggcaatga gaaacataac catggtgaat tagaaaagtt
    14461 gggtgcttat aatggcttat cttatgaaca gatggctcgt ttaggcggtg atccttttaa
    14521 tatgacagag gcggctttgc gtttatgtgc ggtagccaat gccgtttcaa atttgcatgg
    14581 tcatacagcg cgtgctatgt ggcggcatgt tgctgacacg ggtcccatta tttctattac
    14641 caatggtgtg catgtgccga cctggcaggc acctgaaata catcgtgcct ttaagagtgg
    14701 tgcggatttg tggaaaccgc accagcaagc taaacaagaa cttattaact ttgccgaaaa
    14761 acataccgat gccacactta atccaaacgc tttaattgtt ggctttgcgc gtcgggctgc
    14821 cggttacaaa cgttctgatt tgatatttcg taatacagaa gtaattgatc cactcttaag
    14881 agcaggaaaa atgcagctga tcttttccgg caaagcgcat cccaatgaca atgagggcaa
    14941 gcgaattatt cgtgatttag tagctatgga tcgcaagtat aaagattctg tagtttttct
    15001 ggaaaactat aacatggaaa tagcacgttt gttgattaga ggctgtgatg tttggcttaa
    15061 caatcccgtg cgtcccttag aagcttccgg gacttcaggt atgaaagcgg ctatgaatgg
    15121 cgttttaaat ttaagcgttg ttgatggctg ggtaggggag ggaccacagc atggtatttc
    15181 cggctggtta cttgatgctc atcatagcca tattgatccc caacagcaaa atatggatga
    15241 tctgcacgct ctttataagg tactgtttga tgaggtcatt cccacgtact acaacgatcg
    15301 ctcgcgctgg atagatatga tgcgtgcgag tatagatatg tcacatttta agttttctgc
    15361 tacacgcatg gtacgcgaat actatgaatt aatgtatcgt caggtaacgg ataaacagga
    15421 tgccattacg gcgccatgga tggtgcccca gcgttcgcac ggtgagcagc acccctatat
    15481 tacataatag acaaagaggc tgttgcaagc gcggtttatt tgcagcagct tttttgctct
    15541 agtatccgtg cggcaggatt aaagtgactt aacctttgtc aactgctcgt tacttgcatc
    15601 atcctgttag gttttgttta acataaaagg tctttgtatc gtaaaatacc taataatatt
    15661 tattaatctc ctagcataat aggcacacaa tagatcatac taattcaata tatgatgaat
    15721 gggtgagaga atgagtgagg atttttatgt gcaggacaat ctaaaaccaa ctgaggctga
    15781 attagttttt aattctacga ctagcaagaa aaatttaagc ggcgtacgag gaagagcatc
    15841 tcgtactggc cgcacaggcg ccattcgcac gccggttgac ttcttaagtg gtaaagccaa
    15901 aaaagagtat atgaaaagta gcgaggtgaa agtgtataat atgtatgaag atcaagtttt
    15961 accttatgat gaatttaaag agctgcccat cgaaaaacaa gtggttctca tgactagatg
    16021 gcgtgatgaa atcgggacaa acaatattat tcgtgatatg aagatttctc gtaaaaagtt
    16081 ttatagtgaa gtgctaaaaa cgctaggcat tgaaacaaaa cctcgcggca ggcgaccaaa
    16141 gaaaatagaa acagatgagt acaaaacaga gcagacagaa ataatagaga aaaccaaagc
    16201 agaagtgtcc gcagaaatag ttcccgaaac tccggcaaaa gttatcttgg caccagaaca
    16261 gcaagagact tcttttaata tcgtttttaa tgggcgtttc gctggtcacc agttgtctaa
    16321 taggctggca agattagcta gagtcctcga tgctgaagag cagtatgaga ttaaagtaga
    16381 aatacgtgag ctaatttaat tggcaatttt tgacgtgaaa tggtgatatt tcagaatatt
    16441 tgtcggttcc catagattag tgctcttgtg gtcttaggct gcccatcatg ggggtgtgat
    16501 cagccattgg agaaagag
//
LOCUS       DASRMS010000159         2846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_580641, whole genome shotgun sequence.
ACCESSION   DASRMS010000159 DASRMS010000000
VERSION     DASRMS010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2846
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580641"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..2406
                     /locus_tag="VFC74_06865"
     CDS             76..2406
                     /locus_tag="VFC74_06865"
                     /inference="COORDINATES: protein motif:HMM:NF024728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
                     /protein_id="HZK25093.1"
                     /translation="MASLSGGINLQAGEDYQQTASQVVAVEGDIRIQAQQVSIVAGYD
                     TLDHSEKQSSSRTAIGGAVNVPVVDAAISMQQVVRAATHTDDPRMQALAAATVAMQGK
                     TAYDNAKAIVDGDATGIKFSVSLSHEKSKSESTQQSRNVVASSVTAGGDVRIQASGTP
                     NSDVKIIGSTVNAGRDIDLKADRNILLLAAENTAKQNSKNSGSGWSAGIGLGIGGAQN
                     GFSLDLAANQFRGKSDGSDSAWSNTELEAGRQVNLESGQDTELKGAVVKGEQVTAKVG
                     GNLTLESLQDTSTYTSKNSGSSIGASLCIPGFCAGASTVSGSLNSSKVKGDYASVTEQ
                     SGIKAGDGGFQIEVQGHTDLTGAIIASSDSAVENNLNRLETNTLAFSDISNFANAKAT
                     SKGVNLSSDMLFQGKYGVSKGVVGNVLNQGSAKDSSSSQTLSGVSGGEVKIRDEEGQI
                     ERTGQTTEQTISYLNRDTDNSHTPAERLNIQALEREAEAERIIKQAVFAEVVKFTDDA
                     YHTMFIKEHKMYEVILDRNGKPEKNADGTPKIRELSFEEKKNLQANGSDKVHVAANGI
                     FNDPYDAAIYAAQNNPELTGGPLYIVSFPKADSLVAELMVAGYQKHLENNFWGLTNST
                     EEFTGIMRQHGMSGLELDAHSRGSMTIGNAMESLSSLSTTAAGVLSNTFISFFGAAYS
                     AEKAAELLYELSGGKQNTVNLQNHKDDFVGGIIGGNPYSHDRRPENSSKGKEWINMFK
                     NGPTVHSCYGVGKDGCGSAYGVPKTIQVESYGKNKK"
     gene            2387..>2846
                     /locus_tag="VFC74_06870"
     CDS             2387..>2846
                     /locus_tag="VFC74_06870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25094.1"
                     /translation="MEKIKNKILIIFISVFVWGCSSNRSICNIDKPMPDYYRLWEKAG
                     DDEIGIKKKLLECGGKNPSGDMISINDSAITYICMVQAGYFLLVSVNGKRENHNVWCM
                     NWPDLAACQKGANIPKPSIEKRLSSDYCKSARNYQFCKENVVNPAACERMD"
BASE COUNT          912 a          447 c          739 g          748 t
ORIGIN      
        1 tggcagccag cggttgggca ggttaaacat aaaaagcaaa cacaaggcca cactacccag
       61 caaagtggta gtcagttggc ttcattgagt ggcggtatta atcttcaagc aggagaggat
      121 tatcagcaaa cagccagtca agttgtcgct gttgaaggtg atatccgcat tcaggcgcag
      181 caggtcagta ttgttgcagg ctatgacacg ctagatcata gtgagaaaca aagctcaagt
      241 cgcacggcaa taggtggtgc agttaatgtt cctgttgttg atgcagcaat aagcatgcag
      301 caagtagtgc gtgcagcaac gcatactgat gatccgcgta tgcaagcact agcagcagca
      361 actgttgcga tgcaaggaaa gacagcatac gacaatgcta aagcaatcgt cgatggcgat
      421 gcgacaggca ttaagtttag cgttagcttg agtcatgaaa agagtaaaag cgaaagcact
      481 caacagagcc gtaatgtggt ggctagctct gttacagctg gtggtgatgt gcgaatacag
      541 gccagtggaa cacctaatag cgatgtgaaa attatcggta gcacagtaaa cgcaggacga
      601 gatattgacc taaaagcaga tcgcaatatt ttactgcttg ccgcagaaaa tactgccaag
      661 caaaacagta aaaatagcgg cagcggctgg agtgctggaa tagggttagg cattggcggt
      721 gctcaaaatg gctttagttt ggacttagct gccaaccagt ttcgaggaaa gagtgatggc
      781 agtgatagcg catggagcaa tactgaactt gaagcagggc gccaagttaa ccttgagtct
      841 ggccaagata cagagttgaa aggtgctgtt gttaaaggtg agcaagtgac agccaaagta
      901 ggcggtaatt taacccttga aagcctgcaa gacactagta cttacacctc aaaaaacagt
      961 ggttctagta ttggagcaag cttatgtatc ccaggttttt gcgcaggtgc cagtacagtt
     1021 agcggcagtc ttaacagcag taaagttaaa ggtgattacg ctagtgttac tgagcagtct
     1081 ggcataaagg ccggcgatgg aggctttcag attgaggtgc aggggcatac tgatctgact
     1141 ggggctatta ttgctagtag tgatagtgca gtagaaaaca atctgaatcg tttagaaact
     1201 aatactttag ctttcagtga tattagtaat tttgctaatg ccaaagcaac tagcaaaggt
     1261 gtaaatctta gtagtgatat gctttttcag ggtaaatatg gtgtttccaa aggtgttgtt
     1321 ggtaacgttt taaatcaagg ctcagctaaa gatagttcat caagccaaac attaagtggg
     1381 gtaagtgggg gagaagttaa gattcgagat gaggaggggc agattgaaag aactggacag
     1441 acaacagagc agacaatctc atatcttaat cgtgatacgg ataatagtca tacaccagca
     1501 gaacgtttaa atatccaagc tttagagcgt gaggctgaag ccgagcggat tattaaacag
     1561 gctgtttttg ctgaggtagt taagtttact gatgatgctt accatactat gtttattaaa
     1621 gaacataaaa tgtatgaggt gattctggat agaaatggaa agcctgaaaa aaatgcagat
     1681 ggaaccccga agattcgtga actctcgttt gaggagaaaa aaaatttaca ggctaatggt
     1741 tcagacaagg tacatgttgc tgcaaatggt atatttaatg atccttatga tgctgctata
     1801 tatgcagcgc agaataatcc tgaattaaca ggcggcccat tatacattgt gtcatttcct
     1861 aaagcggata gcttggtagc tgagttaatg gttgctggct atcagaaaca ccttgaaaat
     1921 aatttctggg gtttgaccaa ctcaacggaa gaattcacag gaattatgcg gcagcatggt
     1981 atgtcagggc ttgagttgga tgcgcacagt cgaggtagta tgactatcgg caatgcaatg
     2041 gaatcgcttt catctttaag tacaactgct gcgggcgtat taagtaatac atttataagt
     2101 ttttttgggg cagcctacag tgcagaaaag gctgctgagc tgttatatga gttgagtggt
     2161 ggaaagcaaa atacagtcaa ccttcaaaat cataaagatg attttgtcgg cggtattata
     2221 ggagggaatc cttattcaca tgatagaaga ccagagaaca gtagcaaggg taaagagtgg
     2281 attaatatgt ttaaaaatgg tcctacagta catagttgtt atggcgttgg aaaagatggt
     2341 tgtggaagtg catatggggt accgaaaaca atacaggtag aatcatatgg aaaaaataaa
     2401 aaataaaata ttaattattt ttataagtgt atttgtttgg gggtgttcta gtaatcgtag
     2461 tatctgtaat attgataagc caatgccgga ttattatagg ctttgggaga aagctggtga
     2521 tgatgagatt ggtataaaga aaaagttatt ggagtgcggt gggaagaacc catctgggga
     2581 tatgattagt attaatgatt cagcgataac ttatatttgt atggtgcaag caggatactt
     2641 tttacttgtt tctgttaatg ggaagagaga aaatcacaac gtgtggtgta tgaattggcc
     2701 tgacttagct gcgtgtcaga aaggagctaa tataccaaag cctagtatag aaaaaagact
     2761 cagtagtgat tattgtaaga gtgcaagaaa ctatcagttt tgtaaggaaa atgtagtaaa
     2821 tcctgcagcc tgcgaaagaa tggatt
//
LOCUS       DASRMS010000160         2549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_244870, whole genome shotgun sequence.
ACCESSION   DASRMS010000160 DASRMS010000000
VERSION     DASRMS010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2549
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_244870"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1026..2003)
                     /gene="intI2"
                     /locus_tag="VFC74_06875"
     CDS             complement(1026..2003)
                     /gene="intI2"
                     /locus_tag="VFC74_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HZK25095.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIKQLIEQARLIQQDDNLQGVGPSLPFALDHKYPSAYRQAAWM
                     FVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSKRVTCHTFRHSFATHL
                     LQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGLMLLINQ"
     gene            2336..>2549
                     /locus_tag="VFC74_06880"
     CDS             2336..>2549
                     /locus_tag="VFC74_06880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25096.1"
                     /translation="MRKLLHAATIACLAHASLAMAGPGWVLDEKVDPLTDEKIATAMS
                     NYAVGLAQRSAVVRCKGTKLEVYFGFG"
BASE COUNT          723 a          523 c          574 g          729 t
ORIGIN      
        1 tatggcgacc caagatcaaa agcacactga gcagattttg cagttgttac gttaagttat
       61 agtgtttgat gcaataaaag cctagcactt gtagacggac aaaatagttg ggaactggga
      121 ggggtggaaa tggagttttt aaggattatt tagggaagag tgacaaaata gatgggaact
      181 gggtgtagcg tcgtaagcta atacgaaaat taaaaatgac aaaatagttt ggaactagat
      241 ttcacttatc tggttaacta atctagctaa cctgccgctc agcttagtac gatcctatat
      301 ccaactgatc ataatccttt catcagtatc ccaaattaaa tccccctcct agactgcctc
      361 accaacactt tacagtgatc ccgtatttcc ggacaggtga ttgatttttt tcgcttgtac
      421 aggtgttaag ccacagttat cttgatgtgg acattatgca ttgtaatagc ccattaacat
      481 gcgctaagag cataaattaa tatacagtaa caagcattac gaggtgctaa tgtttggaaa
      541 gcagcctctt ccaactacaa taataagtta actatcctcc acccacaaat aattacaaat
      601 gacccctttc cgctataact ccgatctcac cagcggctcg ctacaaaccc gagagtgtcg
      661 cattattact ggcttgttac tccaagagct tgatgaagct gcctgggata aagccatgta
      721 taaggaaaat gtgcttcaga aacgcacgca atctacggta agacgtattt cttcagcgct
      781 tagaaaacgc ctagaacacc taagctctga cttttgggct tttgcgtttt tatgttaggc
      841 attgtctggc aactggagga agtgtaatga ccatgaatgg tcactgtgat tgacagtatc
      901 ttgcttaaac ttatctgaac aatggtagaa ctggctcata tttatcccct ctatgattgt
      961 ttatcttttt caccataaaa agtatcaggg gctactcagg gctacataaa gatatttatc
     1021 tacttttact gattgataag tagcatcagt ccatccgcag gactggtggt gccggcaaaa
     1081 tgctgaccca acacatgcgt atagatttgc gtggtcttaa catcgttatg ccctaagagt
     1141 tcttgcacag tgcgaatatc acgccccgct tgtaatagat gcgtagcaaa cgagtgacga
     1201 aatgtatgac aagtgacacg cttgctaacg atgcctgctt tttgtacggc tgccttcaat
     1261 gcctttcgcg caacggagtc atgcagatga tggcggcata atttgccgtt atacgggtgg
     1321 ttgcagagcg tgctggaggg aaagacaaac atccacgccg cttgtcgata agcagaaggg
     1381 tatttgtgat ctaaagcaaa aggcagcgat ggccctacgc cttgtaagtt gtcgtcttgc
     1441 tgaataagcc gcgcttgctc aatgagttgt tttattgctg ggattaggcg cgtgggcagt
     1501 aggctgtttc tgcttttccc acccttaccg tcatgcacag tgatgcagcc attatcaaaa
     1561 tcaaaatctt taacccgcaa acgcaagcat tcattaatgc gcaaacctgc accatacagc
     1621 agcgtaaaaa taacttggtt gcgagtatcc ataacctgca aaatgcgttg cacttcattt
     1681 gcagagataa cagagggtag ccgtctaggc ttgcttgcag ggatataatc aatatcgccc
     1741 aacggctgtt gtaaaaacct gttgtacaaa aaagctaggg catttaaagc gattttctgc
     1801 gtgtttatgg ctacatgtct gctgtttgct aagctggata aaaacagcct gacctcttca
     1861 ctgcccatgg tctgaggatg acgttttttg tgaaacagaa taaaacgctt aatccagtgc
     1921 aggtaagttt tttcagtttt cagcgcataa cctttttgtc gcatatccgt gcgtatagaa
     1981 tttaaaaatg gactgttaga cataaaacgc tccttgtctt gcaactgtct gcctatacag
     2041 cctattctag ctgggattta aaaaagtgcc tgttttttac gcctagagat gcttgtttac
     2101 cggtagagtt ttaatttaat gctaaataaa ttaaaatgtt atgagttctt tgggtgagat
     2161 aatgtgcatc gtgcaagcag gatagacggc atgcacgatt tgtaataaca gagtgtcttg
     2221 tatttttaaa gaaagtctat ttaatacaag tgattatatt aattaacggt aagcatcagc
     2281 gggtgacaaa acgagcatgc ttactaataa aatgttgggc acttgggaga acacgatgag
     2341 gaaactattg cacgctgcca ccatcgcttg ccttgcgcat gcgtcgctgg caatggccgg
     2401 tccaggctgg gttttggatg aaaaggttga tcctctaacc gatgagaaga tcgccacagc
     2461 tatgtccaat tatgcagtag ggctggctca gcgttcagcg gttgttcggt gcaagggaac
     2521 gaagctcgag gtctacttcg gtttcgggg
//
LOCUS       DASRMS010000161        13653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_646747, whole genome shotgun sequence.
ACCESSION   DASRMS010000161 DASRMS010000000
VERSION     DASRMS010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 13653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13653
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646747"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            399..1124
                     /locus_tag="VFC74_06885"
     CDS             399..1124
                     /locus_tag="VFC74_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HZK25097.1"
                     /translation="MNIAVILAGGNGLRLGAAVPKQFLEILGKPLIVYALQIYERSTY
                     IDAIEVVCIPEYIPVVWEYAKEWGIKKLKWVVPGGASCQDSTRNGIFNLEEVCSNDDI
                     LMLGMSTSVFVDDDIIADSLRVCKQHGNAFAAMQCIYNLAKTSDGLTSTGLEYKEMHK
                     TLNMPWSASFGTFNRLYRKAYAENIETAPTSYAPTLFLALGETLYLSKDTPKNKLHVT
                     TQDDLDIIEACLKYEQIKKGKCK"
     gene            1121..2638
                     /locus_tag="VFC74_06890"
     CDS             1121..2638
                     /locus_tag="VFC74_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HZK25098.1"
                     /translation="MKYLMGIDEGTTGCKACLFNESGELVASASREYPSYYPYPGWVE
                     QDIEQIKDSVFAACKEAIEASGVDPNEILGVSHSNQGITMVLLDENEKPVRDKTIGWQ
                     DLRYVDILPEMQQSVDADEYWKISGMMFGTYNISVLNWLQKNEPETWAKVRRCCSHQD
                     YFLRQYGANGYYIDEGSTNFLSMARLQDNEWDERLMNVYNVNESILPTIVHEPGKVVG
                     FVTEDVSKKTGLPVGCKVCLGGLDTNCCALGAGAKDAGTQVLIMGTAGVSLLISDKMV
                     LDPNKRITVRSNPGFKNWQYYIMTNTGASSFRWFRDEMCSMEVATGKLMGIDPYDIIT
                     TTAAHSHPGANGVTALTCFQGAHTRKKNEKARGTFFGISLGTTKADIAEAILEGICFE
                     MKDILKMNEELSGEIKHVRLCGGVSKSEMWCQMFADILQRPVELTTISELGSLGAAMC
                     AGIGAGLFENCSDAVNKCVHISKTYIPNEKMSKEYAEAFKRWNKYYSIANSEIYI"
     gene            2744..3739
                     /gene="galE"
                     /locus_tag="VFC74_06895"
     CDS             2744..3739
                     /gene="galE"
                     /locus_tag="VFC74_06895"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017413584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HZK25099.1"
                     /translation="MNILVTGGTGYIGSHTCVTLLAAGHDVVIADNLCNSKAETVDII
                     EQISGKKVIFYQIDVTEEAAVESVFAKHAFDGVIHFAGLKAVGESVEKPLQYYYNNTV
                     STMVLAKVCQKYQVKRFVFSSSATVYGENEVPFVETMPLLPTTNPYGETKAISERILT
                     DVVQANPEWSVALLRYFNPVGAHESGLLGEAPNGIPNNLMPYVAQVAKGKLAKLHVFG
                     NDYPTVDGTGVRDYIHVMDLAEGHVVALAKLQPGVHIYNLGTGQGTSVLQLVQAFEAA
                     NQIKVPYEIVARRPGDIAECYADAAKAKKELGWTAKLTLMEMCRDAWRFEQNYRD"
     gene            3808..4293
                     /locus_tag="VFC74_06900"
     CDS             3808..4293
                     /locus_tag="VFC74_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZK25100.1"
                     /translation="MSKIKRTKTILSWALVVLWMCVIFASSSQPALVSAELSTGLTAV
                     LVRTVAKVLQLETPEVEPYLSMFNHLLRKAGHFTVYLILATLVVNALRQSGVTGRRLY
                     LMAFTCCLLYAITDEVHQLFVPGRGGQVRDVLLDSCGALVAILCAYVMSRRKTKDTET
                     A"
     gene            4493..5278
                     /locus_tag="VFC74_06905"
     CDS             4493..5278
                     /locus_tag="VFC74_06905"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF039343.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsB/CapC family capsule biosynthesis tyrosine
                     phosphatase"
                     /protein_id="HZK25101.1"
                     /translation="MRHKIDIHCHILPDVDDGPQTMADSLMLASVLVNQGFAEIIATP
                     HYIDDYSEAYRNHIKQQYKALQTALQVQGIPLQLHLGGEVMLTSLVVQQADSGTLPVL
                     ANTNHVLLELPLRQEVLAYASQVIFHLQIKGYQPIIAHPERVEFIKQDPRCLLRLLES
                     GVLLQVNMGSLFGLYGKRAKQAAEFLLANQMAHFLATDSHHASQGALGHKLTKLAYPL
                     ELLMGENPHNLLNQQRIKQLCKREEKSYSVNKLIRFLKNILGR"
     gene            5298..5972
                     /locus_tag="VFC74_06910"
     CDS             5298..5972
                     /locus_tag="VFC74_06910"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Wzz/FepE/Etk N-terminal domain-containing
                     protein"
                     /protein_id="HZK25102.1"
                     /translation="MEQEIELRELLEIVIKRWKLILLLFLTAVMISAVVSFFLLDPTY
                     QASTTLLVGKPKDDTPVMYQDVQLNRQLVSTYEQIARSGQVGNEVLDELNLSMTLEQL
                     QNKITISQVADTEIIAISVKDKSADKAALLANGVANVFIGKIGAIMKIDNVTIIDPAA
                     VPAKPIAPRKKMNVAIAGVLALMIGFFLAFALEYLDNTVKTAKDVEDYLGLPLLGTIP
                     FYEGEQ"
     gene            5969..6676
                     /locus_tag="VFC74_06915"
     CDS             5969..6676
                     /locus_tag="VFC74_06915"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HZK25103.1"
                     /translation="MRKKRKKTTNPRYAERLITFRNPKSPVSEAFRTVRTNIEFSDID
                     HKLKTIMVTSSIPSEGKSTLTSNIAVAMAMSGKNILIIDADLRSPTQHKCFNVRNLHG
                     LTNLLLDETLSLEDVLVYPSQENLILLTTGPLPPNPAELLGSERMRNFVASLANHFDV
                     ILIDAPPVLPVTDAAILASYVDGVVMVAAAGNVPREQALLAKEQLIKAKANILGVVLN
                     KVSANSKPYYGYYGHSQ"
     gene            6739..8232
                     /locus_tag="VFC74_06920"
     CDS             6739..8232
                     /locus_tag="VFC74_06920"
                     /inference="COORDINATES: protein motif:HMM:NF024686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HZK25104.1"
                     /translation="MKKILLAVLGAALLCAIFVLGQGLFQAEAEPSFVVFSQPGTHGP
                     QQGVAEYLGDITVSSAQTHLQNLKIEGNLYLAPEDEGTIRLTNVDVEGAIVMLAAEET
                     SIYLQDVAAPELTISQAESPVTITTQGSTRIPLLQTRGQAIVQEEEAASGFSELYLNG
                     GSAVEFTGAAVTLTIAGDDAELLLSSGEIAELIVQEGLLRTRIEIGAAGLVKLLDLAA
                     AAKVTGEGTIEEANLHVSNVELELEPLKVTGLQPLTPANTEEDETEEEKAPIIIYRIN
                     NVTLKPGKAALVSVETTPSEQRKLTATSSSSKIVTAAVYQDAIQLRAVAAGTARITVT
                     ASHPDCSDASVTFTVTVPIVQAASPTVSPGSGEVAAGTPLTLATATPGAAIYYTTDGS
                     QPSTNSMRYSASSKPVVSEGGLTLKAIAVSQSLAASQAVTFRFTTPPPVQLPDEEPPG
                     GEEPPTVEEPSDEEDLPPVDDPPEGEEPPPDELEEPPVSEETGQAET"
     gene            8249..9175
                     /locus_tag="VFC74_06925"
     CDS             8249..9175
                     /locus_tag="VFC74_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZK25105.1"
                     /translation="MNKKRWLLAGVLTVVIVLLTGAVVYLRPLYKAYQAVKNPPPAVN
                     EQPNPETEKLLQDRYYNFLVYGVDTGEWVNGTYRPGEARADTIVLMQLDLENKTVAML
                     SIPRDTLVEIPGYAGTDKINHAHAFGGTKLLVETVEHFTNLSVDYFIQINYPLFRDVV
                     EAIGGVEFEVDRAIKARGLQLEPGLQVLNGDQAFALVSFRYEAMGDIARVQRQQRFLL
                     ALARQVKTLSTGELLPAFYAAWQQIKTNLSSQEAAELVMLLNGLQVNTVTTEIVPGWF
                     YNRSGVSYWRPNEEETKALITNIFRHTDKTKS"
     gene            9397..9879
                     /locus_tag="VFC74_06930"
     CDS             9397..9879
                     /locus_tag="VFC74_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25106.1"
                     /translation="MDLRVNKRQLLLIGVDALLLTAAAYFGLLLQFNFQFTTFYLAQL
                     NAQIFLIVPLCLLTFFLCGLYRPLWRYASIHELLLVVAAVTSSDLLIYLYLELFLATP
                     LPTSFYIIKWILNLGLIGGSRMLMRILPTRFQYFHSAESRVLIIGAGAAGIMVAKEIQ
                     "
     gene            9916..11220
                     /locus_tag="VFC74_06935"
     CDS             9916..11220
                     /locus_tag="VFC74_06935"
                     /inference="COORDINATES: protein motif:HMM:NF014740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HZK25107.1"
                     /translation="MDDDDAKQRCGISGVKVLGTREDIPQLVQQHKVTEVIIAIPSAP
                     RSEIKKLTRICSQLAVQVKIIPGVHQLINGTVSYSRIRSVQIEDLLGREEIKTNLAEI
                     AQYLQHETVLVTGAGGSIGSELCRQIVQFNPHQLLLLDHTENNVYDIEMELRQLHLDC
                     TVVPIVADVRNYQRIEDVFTEYQPTIIFHAAAHKHVPLMEVNCKEAIENNIGGTKNVA
                     EAAHRHRAKSFLLISTDKAVHPTSVMGATKRAAEIIIQMIAEKSPTHYCAVRFGNVLG
                     SRGSVIPLFKKQIAAGGPVTVTHPEMTRYFMTIPEAVQLVIQAAAMGNNGNIFILDMG
                     EPVKISDLAHDLIRLSGFEPEKDIQITYTGIRPGEKLYEELHSAEEDTHTTTHERIFI
                     AQPIDQSSDSFLQELEHLQKILHTDLREYYLNKLAVKKYNVG"
     gene            11323..12561
                     /locus_tag="VFC74_06940"
     CDS             11323..12561
                     /locus_tag="VFC74_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZK25108.1"
                     /translation="MTTNLSIALLDLVTCLAEAVDLISPSLAHHHQQVAFIAYSLAQE
                     LQLTPQAQAELYLAGALHDIGALSLQERIGTLQFEFEEGTEHERIASDLLQQFPPLQP
                     IARIIRHHHTYWEHGEGEFAPLASHIIHLADRVAVLIKKERNILSQKESILARISAQK
                     NKMFHPELVQALHSLAKKESFWFDTIYDTNFDQSLQPTFITLNLEQLAQLAALFAKVI
                     DFRSPFTATHSAGVAATAAKLAELAGFKQHEVQLMHVAGHLHDLGKLAIPKEILEKPG
                     KLTPEEYNLIKSHTYYSYRLLQNIPGLETINTWASLHHEHLDGSGYPFKLSADKLTPG
                     SRIMAVADVFTAIAEDRPYRRGMAEDEMLRILYSLAAKNHLDQEIVELLQENLPAIND
                     TRLQAQELAQQSYGRVITNS"
     gene            12885..13250
                     /locus_tag="VFC74_06945"
     CDS             12885..13250
                     /locus_tag="VFC74_06945"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HZK25109.1"
                     /translation="MSQEGFTLIEMTVVLAILSIILVTVTPLFADYMERSKTNVCSIN
                     RQLLFKEYHLYLVMNGIKHSNLVLDKYILENGQNICPKQGSISAGEDIIQCSIHDKYE
                     DKDDPGDGGEGDDDQVPYL"
BASE COUNT         3914 a         2927 c         3199 g         3613 t
ORIGIN      
        1 gtattccagg ctgtttattt catatctctg ccgaagatgg ctatagcctt tttaaaatgg
       61 aattctcctc tttgaaattc ttaattcttt cattgcagcg ttacctggac gaatatcaac
      121 tatactagaa ttttgatact cttttagtaa atgtttttat ttatgccgtg acacgtgctt
      181 tttcggggac attattggac ctactccatg tacggaactt aggtacttgc ttattggttc
      241 tagtccccga agtagaagta actaacggta tccaatagct tttagtttta agtattatgt
      301 ttttgaagca atagcattaa ttttttgtcg tcaatatttc gggtaacaac gtggttaact
      361 ttctaacaaa taaaaaacta tgaggagcag gaataatcat gaatatagca gttattttag
      421 ctggtggcaa tggattgcga ttgggggcag cggtgccgaa acagttttta gagatactag
      481 gaaaaccttt gatcgtgtat gctcttcaaa tttatgagcg ctccacctac attgatgcga
      541 ttgaggttgt ctgtatacca gaatatatcc cagtggtatg ggagtatgcc aaagagtggg
      601 gtataaaaaa gctcaaatgg gtagtgcccg ggggtgcatc ctgtcaggat tcaactcgaa
      661 atggaatctt taaccttgaa gaggtttgct ccaatgatga tattctgatg cttggaatga
      721 gtacaagtgt gttcgtagat gatgacataa ttgccgattc tctgcgggtg tgcaagcagc
      781 atggtaacgc ttttgctgca atgcagtgta tttataactt agcaaagaca tctgacggct
      841 taacatcaac tggacttgaa tataaggaga tgcacaagac attgaatatg ccttggtcgg
      901 cttcgtttgg tacatttaat agattatatc gtaaggctta tgcagaaaac atagaaactg
      961 cgcccacatc atatgcacca actttgtttt tggctctggg cgagacactg tatttgtcaa
     1021 aggatactcc taaaaataaa ctccatgtta ctacgcaaga tgatcttgat ataatcgagg
     1081 cctgtttgaa atatgagcaa attaagaaag gaaaatgcaa gtgaaatact taatgggaat
     1141 tgatgaggga accacagggt gcaaagcgtg tctgtttaat gaatcaggcg agctcgtggc
     1201 tagcgcatcc agggagtatc cgagctatta tccatatccc ggttgggttg aacaagacat
     1261 cgaacaaatt aaagacagcg tatttgcagc ttgcaaagaa gcaatagaag cttctggtgt
     1321 ggatcccaat gaaatcttgg gggtaagcca ttccaaccag ggcataacga tggttctgtt
     1381 ggatgaaaat gagaagccag ttagagacaa gacgattgga tggcaagact tacgctatgt
     1441 tgatatcctt cctgaaatgc agcaaagtgt tgatgctgat gagtattgga agattagcgg
     1501 catgatgttt ggtacctata atatctctgt gctgaattgg ctacaaaaaa atgaacctga
     1561 aacgtgggca aaggttcgac gttgctgttc tcatcaggat tattttctca gacagtatgg
     1621 cgcaaatggt tattatattg atgagggcag caccaacttt ttaagcatgg ctcgtttgca
     1681 agataatgag tgggacgagc gtcttatgaa tgtgtacaat gttaatgaat caattttacc
     1741 cacgattgtg catgagccgg gtaaggttgt tggttttgtt acagaagatg tctcgaaaaa
     1801 aaccggtctg cctgtaggtt gtaaagtgtg tctcggtgga ctcgatacaa attgctgtgc
     1861 gctgggagcg ggtgcaaaag atgctggtac acaagttctt atcatgggga cagcaggtgt
     1921 ttccctcctt atatctgaca aaatggtgct cgatccaaac aaaagaatta cggtcagaag
     1981 caatccgggc tttaaaaact ggcagtatta tatcatgacg aataccggag cgtcttcttt
     2041 ccgatggttc cgtgatgaaa tgtgctcaat ggaagttgct actggtaagc tcatgggtat
     2101 agacccatac gatattatta caacgactgc ggcgcactcg caccctgggg ctaatggtgt
     2161 aactgcactt acttgctttc agggggcaca tacgcgtaag aagaatgaaa aggccagagg
     2221 tacctttttt ggcattagtc ttggcacaac aaaagcagac attgcggagg cgatactaga
     2281 gggcatttgc tttgaaatga aggatattct gaaaatgaac gaggaacttt ctggtgagat
     2341 caaacatgtc aggctttgtg gaggagtatc aaaaagtgag atgtggtgcc agatgtttgc
     2401 ggatatcttg caaagacctg ttgagcttac aacgatatcg gaactcggtt cattgggagc
     2461 tgctatgtgc gccggcatag gtgctggatt attcgaaaat tgttctgatg ctgttaataa
     2521 gtgtgtccac atcagtaaaa cctatatacc taatgaaaag atgagcaaag aatacgcaga
     2581 ggcattcaag cgctggaata aatactactc gattgctaat agtgagatat atatttgatg
     2641 actgaaaaaa tccgattgtc agcttgtgat agtcggagta ttatcccgtt gcatgttcag
     2701 tatatcagat taaatagtaa agtattctgc aaaggaggct acaatgaaca tcctcgtcac
     2761 cggtggtacc ggctacatcg gctcccatac ctgtgtcacc ttgctggcag cagggcatga
     2821 tgtagtaatc gctgataatc tttgcaacag caaagccgaa accgtagata taattgaaca
     2881 aattagcggc aagaaagtta ttttctatca gattgatgtc actgaagagg cagctgttga
     2941 gtctgtcttt gctaaacatg cctttgatgg ggtaattcat tttgctggac ttaaagcggt
     3001 gggtgagtcg gtagagaaac cactgcaata ttattacaac aacaccgtta gtacgatggt
     3061 gctggcgaag gtgtgccaga agtaccaagt aaagcgtttt gtctttagct cctccgctac
     3121 tgtgtacgga gaaaatgaag tgccttttgt ggaaaccatg cctctcctgc ctacgactaa
     3181 cccttatggt gaaactaaag ctatcagtga gcgcattctc acggatgtgg tacaagctaa
     3241 tcctgagtgg tcagtagcgc tgctgcgcta ctttaacccc gttggtgcac atgaaagcgg
     3301 cttactcggt gaagcaccga acggtattcc taataacctg atgccctatg tggcacaagt
     3361 agctaagggt aagctagcga aattacatgt ttttggtaac gactatccta ctgtagatgg
     3421 taccggtgta cgggattata tacacgtgat ggatctagcc gaagggcatg tggtagcatt
     3481 agctaaactg cagcccggtg tgcacatcta taatttaggt acgggacaag gcaccagtgt
     3541 gttacagttg gtgcaggctt ttgaagcagc taaccagatt aaggtgccct atgaaatagt
     3601 ggccagacgc cctggtgata ttgccgaatg ctatgccgat gccgccaagg ccaaaaaaga
     3661 attgggctgg acagctaaac tcactctaat ggagatgtgt cgcgatgcct ggcgctttga
     3721 gcaaaactac cgcgattaag cggtcgccta aattatcaag tagtacttta agctgagtgg
     3781 cataaaataa agaaaagaat atagttggtg agcaagatta aacgaacaaa aacaattctt
     3841 tcctgggcat tggtagtgct gtggatgtgc gtgatttttg ccagttccag tcagccagca
     3901 cttgtatccg ctgaattaag tactggtcta acggcagtat tggtacgcac agtagccaaa
     3961 gtactgcagc tagagactcc cgaggtagaa ccttacttga gtatgtttaa ccatctactg
     4021 cgtaaagccg gtcactttac cgtgtactta atattagcca ctttagtagt gaacgccctg
     4081 cgacaaagcg gtgtgaccgg gcgcagatta tatctcatgg cttttacctg ctgcctgctt
     4141 tatgccatca cggatgaggt gcatcaacta tttgtacctg gccgcggcgg gcaggtgcgt
     4201 gatgttttgc ttgatagttg tggggctttg gtggccatac tgtgtgccta tgtcatgagc
     4261 cggcgcaaaa ctaaagatac tgagacagct tgatctagta agttgcgcat agtaatgaga
     4321 aataaagaag catcagaatg actcactaat gggtcatttt tcatttagcg ctaaggatta
     4381 gcaagtaacc gtgctgtcaa aatgcagtaa gcaacaaaat gggtgtgatt taggcaggat
     4441 aagaaaaata tgtgtcgaaa taaaataaac ataagaaaaa cgaggtaata atatgcgaca
     4501 taaaattgat atccactgcc acattctccc ggatgttgat gatggacccc aaacgatggc
     4561 agactcttta atgctagcta gtgtgctagt caaccagggt tttgcagaaa ttattgccac
     4621 ccctcattac atagacgatt attctgaggc gtaccgcaac catattaaac agcaatataa
     4681 ggcactgcag acggcgctgc aggtgcaggg catccctctg cagctacatt taggcgggga
     4741 agttatgctt acttccctag tcgtacagca agctgatagt ggtaccttac ctgtgcttgc
     4801 taataccaat catgtgttat tagagctgcc tttaagacaa gaagtgttag cttatgcctc
     4861 acaggtaatt tttcacctgc aaattaaagg ctatcagccg attattgccc atccggaaag
     4921 agtggaattt attaaacaag atcccaggtg cttactgcgc ctgctcgaat caggggtgct
     4981 gctgcaagtg aatatgggaa gtttatttgg cctttatggg aaaagagcca aacaggcagc
     5041 agaatttctc ctggctaacc agatggcaca ctttttagct actgatagtc atcatgcttc
     5101 ccaaggggca ctcgggcata aacttacgaa attagcctat cccttggagc tgttgatggg
     5161 ggagaatcca cacaacctct taaatcagca aaggattaag cagctgtgca aaagggaaga
     5221 gaaaagttac tctgttaata aactgattag attcttaaaa aatatcttag gcaggtaaga
     5281 aggagagcag aagaaacttg gaacaggaaa ttgagcttcg cgagctactg gaaatcgtga
     5341 tcaaacgttg gaagttaatt ttgctattat tcttaaccgc agtgatgatt agtgctgtgg
     5401 taagcttttt tttgctagac ccaacctatc aggcttcaac caccttatta gtgggtaaac
     5461 ctaaagatga tactccggtc atgtatcagg atgtgcagct caaccgacag ctggtttcca
     5521 cgtatgagca aattgcccgt agcgggcaag taggtaatga agtgcttgat gaattaaacc
     5581 tgtcaatgac tttagagcag ctgcaaaaca agattacgat tagccaagtg gctgatacag
     5641 aaattatcgc cattagtgtg aaagataaat cagccgataa agcagcttta ctggcgaatg
     5701 gtgtggccaa tgtcttcatt ggtaaaatcg gtgccattat gaaaatagat aatgtcacca
     5761 ttattgatcc ggcagctgtc cccgccaagc ccatagcacc gcgtaaaaag atgaatgtcg
     5821 ccatcgctgg agtgctggcg ctgatgattg gcttcttttt agcctttgcc ctcgaatatc
     5881 tggataacac ggtgaaaaca gcaaaagatg tggaagatta tttaggttta ccgttattag
     5941 ggacaatacc tttttatgag ggagaacaat gagaaaaaaa agaaaaaaaa ctacaaaccc
     6001 acggtatgcg gaacgtttaa ttaccttccg caaccctaag tctccagttt cggaagcttt
     6061 ccgtaccgtg cgtactaata ttgaattctc tgatatcgat cataaactca aaacaattat
     6121 ggttaccagt tccatcccgt cagaaggtaa gtctaccctg acttccaata tcgctgtagc
     6181 catggccatg tcaggcaaaa acattctcat tattgatgcg gacttaagaa gtcctacgca
     6241 acataagtgt tttaatgtcc gcaatctgca tggcctgacc aatctgctcc ttgatgagac
     6301 actttcactg gaagatgtgc tggtttatcc tagtcaggag aatttaattt tgctaacgac
     6361 agggccgctg ccgcccaatc ccgctgaact tttaggctca gagcgcatgc gcaattttgt
     6421 agcctctctg gctaatcatt ttgatgtgat tctgattgat gccccacctg tcctgcccgt
     6481 gaccgatgcc gctattctcg caagttacgt cgatggtgtg gtgatggttg ctgccgccgg
     6541 taatgtgccc cgcgaacaag cgttactggc aaaagagcag ctcatcaaag ccaaggccaa
     6601 tattttaggg gtggtcctga ataaggtttc tgccaatagt aagccttatt atggttacta
     6661 tggccattca caataactag tcgtttagtt ttaaggagat ttaaatagct gcaaaaagta
     6721 attaaggtgt gtagatcaat gaaaaagatt ttattagccg tgttaggcgc ggccttatta
     6781 tgtgccatat tcgtactagg ccagggtctg tttcaagccg aagcagagcc ctcttttgta
     6841 gtcttctcac agccaggcac acatggtcca caacaaggtg tggcagagta ccttggtgat
     6901 attacggtat cttctgccca aactcatctg cagaacctaa aaattgaggg aaatctttac
     6961 ttggcacctg aagacgaggg cacaattaga ctaactaatg tggacgtaga gggtgctatt
     7021 gttatgctgg ccgcagagga gacaagcatc tacctgcagg atgtggcagc acccgaacta
     7081 acaatcagtc aagctgagag ccccgtcacc attaccacgc agggttccac ccggattccc
     7141 ctccttcaga ctcgggggca agccattgtc caggaagaag aagccgccag cggcttcagc
     7201 gagctttacc ttaacggtgg gtctgctgtg gagtttacag gcgctgcggt caccctgact
     7261 attgctgggg acgatgcaga actacttttg agcagcgggg aaattgctga actaatagtg
     7321 caggaagggt tgctgcgcac gcgtattgaa attggtgccg ctggtctggt taaactgctg
     7381 gatttagcgg cagcggcaaa agtcaccggc gaaggcacta ttgaagaggc taacttacat
     7441 gtttctaatg tcgagctgga gctcgaaccg cttaaggtta ctggtcttca gccccttaca
     7501 ccagcaaata ctgaagaaga tgagacggaa gaagaaaaag ctccgattat catctaccgt
     7561 attaataacg taacgctaaa gccgggtaaa gccgcactag tctccgtcga gaccacgccc
     7621 agtgaacaga gaaaactcac tgctacttcg tccagcagta agatagttac agctgcagtt
     7681 taccaggatg ctattcagct gcgcgctgtg gctgccggga cagcaaggat tactgtaaca
     7741 gccagccacc cagactgcag cgatgcctcc gtaaccttta ccgtaactgt acccattgtg
     7801 caggctgcca gtcctacggt tagtcctggc agcggtgaag ttgccgcggg tacgccgcta
     7861 actctagcga cagcaacacc cggtgccgct atttattata ctactgatgg cagtcagcct
     7921 tccacaaata gcatgcgcta cagtgccagt agtaaacctg tcgtcagcga aggtggtctg
     7981 acactcaagg caattgctgt cagtcagtct ttagccgcaa gtcaagctgt gacctttaga
     8041 tttactacac cgccacctgt gcaactgccg gacgaagaac caccaggggg ggaagaacca
     8101 ccaacggtag aagagccgtc tgatgaggaa gatctcccac cggtagatga cccacctgag
     8161 ggagaagaac caccaccaga tgagctagaa gaaccgcctg tgtctgagga aacaggacaa
     8221 gcagaaacat agattaacgg gggttatgat gaacaagaaa agatggttac tggctggtgt
     8281 gttaactgta gttattgtgc tgttaactgg tgcggtggtt tacctaaggc cgctttacaa
     8341 ggcctatcag gctgtaaaaa acccgccacc tgctgttaac gaacaaccaa atcctgaaac
     8401 agaaaaactg ctgcaagacc gttactataa ctttttagtc tatggtgtag atacgggaga
     8461 atgggtaaat ggtacttatc gccccggtga ggcgcgtgcc gataccattg tcctcatgca
     8521 gttagacttg gagaacaaaa ctgtggcaat gctctccatt cctcgtgata cgctggtcga
     8581 gattcccggt tatgccggaa ctgataaaat taatcatgcc catgcctttg gtggtaccaa
     8641 acttttagtc gagacggtag agcactttac taatctatcg gtagactatt tcatccagat
     8701 caattacccc ctctttcgcg atgttgttga ggctatcggg ggtgtggaat ttgaggttga
     8761 tcgtgccatc aaggctcgtg gtctacaact ggaaccaggc ttgcaggtct taaatggtga
     8821 ccaagctttt gccctggttt cttttcgtta cgaagccatg ggtgatattg cccgtgtgca
     8881 gcggcagcag cgtttcctct tggcgctggc ccggcaggtg aagacacttt ctacggggga
     8941 actgctgccg gctttttatg ccgcctggca gcagataaaa acaaacctct cttcacagga
     9001 ggcagcagaa ttagtgatgt tactcaacgg tttacaagtt aacacagtaa caacagagat
     9061 agtccccggc tggttttaca accgcagcgg tgttagttac tggcgcccta atgaggaaga
     9121 aactaaagca ctaataacca atatttttcg ccacacagat aaaactaaaa gttgaaaaat
     9181 ggaagataaa gaggaaggaa aagccaaaac tgtgtagaaa tatagtatac aatggaaaat
     9241 attataacaa tccatggcaa atgatgctag atgttgacat ttctggtttg aggatgaggc
     9301 tgcgcagaaa gaaagtcgct taaaactgtc gaattcctac tctagaaata aatcaggata
     9361 ttaatcatta gggatcgcgc ttcgggggga gaatctatgg atttacgtgt aaacaaaagg
     9421 cagctgctgt tgataggggt agacgcttta ttgctcacag cagctgctta ttttggcttg
     9481 ctgctgcagt tcaatttcca atttactact ttttatctcg cacaattaaa tgcgcaaata
     9541 tttttaattg tgccactttg tttgcttact ttttttctct gcggtctcta ccgaccactc
     9601 tggcgttatg ccagtattca tgaattgctg ttagtagttg cagctgtcac cagcagtgat
     9661 ctgctgattt atctttattt agagctattt ttagctacac cgctgcctac cagcttctat
     9721 attattaaat ggattctcaa tcttggtctg ataggcggca gccgcatgtt aatgcgcatt
     9781 cttcctactc gctttcagta ttttcacagt gccgaaagcc gagttctgat tattggtgcc
     9841 ggtgcggccg gtatcatggt ggccaaagaa atacagtagc agtttgccag cctcaaaaag
     9901 cttttaatcg gctttattga tgatgatgac gccaaacagc gctgcggcat ttccggggtc
     9961 aaagtcttgg gcacccgtga ggatataccg cagctggtgc aacaacataa ggttaccgaa
    10021 gtgatcattg ctattccttc cgcaccccgc agtgaaataa aaaagctgac ccgcatttgc
    10081 agtcagctgg ctgtacaagt taaaattatc cccggtgtgc accaactaat taacggcacc
    10141 gtctcctaca gcagaattcg ctccgtgcag attgaagatc ttttaggccg ggaagaaatt
    10201 aaaactaatc tcgcggaaat tgctcaatac ctacagcacg aaaccgtttt agtcaccggc
    10261 gccggcggct ccattggctc tgaactgtgc cgccaaattg tgcagtttaa cccccaccag
    10321 ctcttattat tagatcatac ggaaaacaat gtctacgata tcgaaatgga actgcgccag
    10381 ctccacctag actgcactgt cgtacccatt gtagccgatg tgcgcaatta ccagcgcatt
    10441 gaagatgtct ttactgagta tcagcccacc atcatcttcc atgccgccgc ccacaaacat
    10501 gtgccgctga tggaggttaa ctgcaaagaa gcgatagaga acaatatcgg cggcaccaaa
    10561 aatgtcgcag aagcagcgca ccgccaccgg gcaaaaagtt ttttgctcat ctctacagat
    10621 aaagctgtac atcctaccag cgtgatgggg gccactaaac gcgctgcgga aatcattatc
    10681 cagatgatcg cagaaaaatc gccaacccat tactgtgccg tgcgcttcgg taacgtttta
    10741 ggttcccgcg gcagtgttat tcccctcttt aagaaacaaa ttgccgccgg tggtcccgtt
    10801 actgttaccc atccggagat gacccgttac tttatgacca ttcctgaggc ggtacagctg
    10861 gtcatccaag ccgccgccat gggtaacaac ggcaatatct ttattctgga catgggtgag
    10921 cccgtcaaga tcagcgacct cgcccacgat ctaattcgcc tttccggttt tgaaccggaa
    10981 aaagatatcc agattaccta caccggtatc cgccccggcg aaaaactcta cgaggaactg
    11041 cactccgccg aagaagacac ccataccacc acccacgaac gcatcttcat cgcccagccc
    11101 atagaccaaa gctccgactc tttcttgcag gagcttgaac acctgcaaaa aatcttacat
    11161 acagacctgc gggagtacta cctcaataag ctggcagtaa agaagtacaa tgtggggtaa
    11221 gtaattaata tcaacaacta gttgataagc agcacgcagt caagcacatt actatgaaca
    11281 atcaaaatac caactaatat acattgatga ggatctacca ccatgacgac aaatctctcc
    11341 attgctttac tcgatcttgt cacctgcctg gctgaggcag tcgacttaat tagccccagc
    11401 cttgcgcacc accatcagca ggtggccttt atcgcctata gtcttgccca ggagttacag
    11461 ctaaccccac aggcacaagc cgagctctat ttagcaggtg ccctgcacga tatcggtgcg
    11521 ctttcgctac aagaacgcat cggcacccta cagtttgaat tcgaagaagg aaccgagcac
    11581 gagcgcatcg ccagcgacct cctgcagcag ttcccacctt tgcaaccaat cgcccgcatc
    11641 atccgtcatc atcataccta ctgggagcac ggcgaaggtg agtttgctcc ccttgccagt
    11701 cacatcatcc atctggctga ccgtgtcgct gttctaatta aaaaagaacg caacatcctc
    11761 agtcaaaaag agtccatcct tgcacgtatt tcagcacaaa aaaataaaat gttccacccg
    11821 gaactagttc aagctcttca ctcactggcc aagaaagaaa gtttctggtt cgataccatt
    11881 tacgatacta attttgatca gtctctgcag cctactttta tcaccctcaa cctggagcaa
    11941 ctcgcccagt tagccgcgct ctttgctaaa gtcatcgact ttcgcagtcc ctttaccgct
    12001 acccattctg ccggtgtcgc tgctactgcg gccaaactag ccgaacttgc cggctttaag
    12061 cagcatgagg tacagttgat gcacgttgct ggtcacctac acgatctcgg taaactggct
    12121 atccccaaag agatcctaga aaaaccaggc aagctcacac cggaggaata taacctgatc
    12181 aaaagccaca cctattattc ataccgcctc ttgcagaaca tccccggcct ggagactatc
    12241 aatacctggg cctctctgca ccatgaacat ctcgacggta gcggctaccc ctttaaatta
    12301 tcagcagaca aactaacccc cggctcccgc atcatggccg tggctgatgt ttttaccgcc
    12361 attgccgaag accgtcctta ccgccgtggg atggcggaag atgagatgct gcggattctg
    12421 tacagcttag ctgccaagaa ccatttagat caggagatag ttgagttgct gcaggagaat
    12481 ttgcctgcta ttaatgatac tcgtctgcag gcgcaggagc tggcgcagca gagttatggt
    12541 agagttatta ctaatagttg atgcaaccaa gtcagcatac cgaccacaat ctgtagaaca
    12601 gatattaaaa agatactatt gtagttaatc atgtgagtcg tgagtatggg gaagggataa
    12661 agtacaaaat tagttcctag gactttaaaa cacaaattag tgacatatgt tttatttgct
    12721 taatggggtg aacgcagatg tatatgcagc aatcaatgca aacagaaatg aagagacgca
    12781 tctttgtgtc tttttttgcc ttttagctaa tttgaaagcg gtcgtataag cattttgagt
    12841 aagtatatta caacatctat accagtgtta ttaggtgggg cggtatgagt caggaaggat
    12901 ttactttgat agaaatgaca gttgtcttag caatattatc gataatatta gtaactgtga
    12961 ctccattatt tgcagactat atggaacgtt cgaagacaaa tgtgtgttca attaataggc
    13021 agttactctt taaagaatat catttatatt tggtaatgaa tggtattaaa cattctaatt
    13081 tagtgcttga caagtacatt cttgagaatg ggcaaaatat ttgtccaaag caaggttcta
    13141 taagcgcagg agaagatatc attcaatgta gcatacatga taagtatgaa gataaagatg
    13201 accctggtga tggtggagaa ggtgacgatg accaggttcc atatttgtag gagattggat
    13261 ggttagagcg acgtgagtat aaagatgtga tggaatacta tatgttgtgt tttggagagg
    13321 aaaaatgtta agatatatac aatatataga ttagtccttt tatcataaac gtatcgaagg
    13381 tgatacgaaa aggataaaaa acgagtgagt atgtgtaaag gacaagtaaa aatcaacaaa
    13441 cacaatatgt agatgtagtt aaatcaacgc atacaacatg ttgcataaaa atcgaatgat
    13501 tgtatggacg ctatcgacgt gagtatcgga aaagaataac aagggtgcag atgtagtaat
    13561 agcaagggtt ttataggtta ttaaaatgag agaaatgtta cacaatggtt acaggtataa
    13621 ccattcaatc atcgaaagtg ctgaatgcat tgg
//
LOCUS       DASRMS010000162         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_781440, whole genome shotgun sequence.
ACCESSION   DASRMS010000162 DASRMS010000000
VERSION     DASRMS010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_781440"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            828..1628
                     /locus_tag="VFC74_06950"
     CDS             828..1628
                     /locus_tag="VFC74_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25110.1"
                     /translation="MTEKLNVMFEESTSENTPVGTKSLEGTAQLTSIANTIAQELIKT
                     ISSDVETHGEMFEKSKEDHNAMDDLVKALYDLSVVETEFLKELGEDVLNGMLKSQQSK
                     RSRSKGKTMTIENYRNMMSGAIAELLLRDVLGKPKAATGYRRASGSLEYSDEEIEMYR
                     NDQDLLRRELRNVQSKKSIEKRKEDFSEESERWKQLIVAEDILKSMRVGGTSSRTVIV
                     DKTGEQLKAELGDRDVNDLKAAEAKALLKKLLEAAGGEAAQEVEHRDE"
     gene            1621..1791
                     /locus_tag="VFC74_06955"
     CDS             1621..1791
                     /locus_tag="VFC74_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25111.1"
                     /translation="MNKIWLETLAECCRCYPDKQGNMPCDNGRLCDACEQEWVQEVYQ
                     QKKRKDAAAKTE"
     gene            2134..2421
                     /locus_tag="VFC74_06960"
     CDS             2134..2421
                     /locus_tag="VFC74_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25112.1"
                     /translation="MNKSERDSMNEANELSRLMKAMTKQMQSDNDRYEENPDADFNIS
                     NITITVGDETIRLVLGGPQAAGIHSMIQHIASENMYCVDFDRDEVHDYCSK"
     gene            2689..2847
                     /locus_tag="VFC74_06965"
     CDS             2689..2847
                     /locus_tag="VFC74_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25113.1"
                     /translation="MNKLTGASYSAIHDAICNQLDYLAGGEQYELLLQALNELNAIYY
                     DEGVNNND"
     gene            2840..3043
                     /locus_tag="VFC74_06970"
     CDS             2840..3043
                     /locus_tag="VFC74_06970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25114.1"
                     /translation="MISLRLTDVAESYREMLHTIGADADVYAAIMNNFYKLCETSGVG
                     DKTAVQSLYPGITDEEYYDGWSE"
     gene            complement(3315..3833)
                     /locus_tag="VFC74_06975"
     CDS             complement(3315..3833)
                     /locus_tag="VFC74_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25115.1"
                     /translation="MTDLKKIEKAANELKTVENVNRELKRIASVKCRLKKQKGRADYS
                     DKMTEILQQEQILKEAKNLIKPAKKTVTTYTQNDVDLIEDYDELAKAIRTIQSKKTLS
                     RWLTTIEGDNDEFRNAEKIERMLIERRKLIKPVDDVNIRKTDVQKIIDTIESSGNISQ
                     GKIVELLKNVIQ"
BASE COUNT         1282 a          894 c          978 g         1117 t
ORIGIN      
        1 aatatataca tatatatata tatatatata taaataatct gtttagttac atatattgat
       61 tctatatcca atacctgttt atttttcaac cccgcagctt aattataaac atgtgtagaa
      121 accagaccaa gaatcctcta acctgacgcc gcgccgggtc gacgcgcaac agaaatccaa
      181 aataactgtg cgggctttta tgcggcgaca cgcggtaaag cccaattgta gttactcggc
      241 agtgcgctac agaacaggtg gccaagacgc gccgctgggg cgatttgaag tggagtagta
      301 tagttcagtt cgtcaaagct tttatgcggc gcagcggcgc ttagaccgat tggcgcttta
      361 tgaagtaagg tatagtcctg cctgaccacg gcggtgcggc gttatatggt tcagactgac
      421 catggccgca tggcttacgc tcgccgagct tgtccacaaa ctagtagata gcccggcgct
      481 ggcgcgctga ggctaaacgc cctgggaaga ggtgtcgccg cggtcatgca ggactatatt
      541 ataacttctt tgcgcggtga gtaaataatt tttctaaaac tggatacatt ccacaagaat
      601 tacgttataa ttatattaag agtaaggaca agaataaaaa caagacacct gtagttatat
      661 catgtgcaga agcaagctga gcggctacca cattgaatta agaataacat atgtacggtc
      721 gcacggttcc atgatataat ataattaagg gtgaggcaat tactctaata cgcaggccac
      781 tgcaaagtgt ggcatccgct tggatgaagc ggcgaaagga gactaccatg actgaaaaat
      841 tgaatgtaat gtttgaagag agcaccagcg aaaacacacc tgtaggtaca aagagtttag
      901 agggtaccgc acaactaaca agtatcgcaa acacaattgc acaggagtta atcaaaacaa
      961 ttagcagcga cgtggagaca catggcgaga tgtttgaaaa gtcaaaggaa gaccacaacg
     1021 caatggacga tttggtaaag gcgttgtatg acctcagcgt tgttgaaact gagttcctca
     1081 aagagcttgg cgaggacgta ctaaacggca tgctgaagag tcagcagtca aagcgttccc
     1141 gcagcaaggg taaaacaatg acgattgaaa actacagaaa catgatgtca ggcgcaatcg
     1201 ctgagttgtt actgagggat gtgttgggca aacccaaagc agcaacgggc taccgcaggg
     1261 cctcagggtc attagagtac tccgacgaag agattgaaat gtatagaaac gaccaagact
     1321 tgctgagacg tgagctccgc aatgttcagt ccaagaagtc tattgaaaaa cgcaaggaag
     1381 acttcagcga agagtctgaa agatggaaac aactaatagt cgcagaagac attctgaaga
     1441 gtatgagggt cggcggcaca tcatccagga cagtcattgt agataaaact ggcgaacaac
     1501 tgaaagctga actaggtgac agagacgtaa acgacctaaa ggccgctgag gccaaggcgc
     1561 tgctgaagaa attacttgaa gccgctggag gcgaggcggc acaggaggtg gaacacagag
     1621 atgaataaaa tatggcttga aacactagcg gagtgctgca gatgttatcc agacaagcag
     1681 ggcaatatgc cctgcgataa cggtagacta tgtgacgctt gcgaacaaga atgggtacaa
     1741 gaagtgtacc aacaaaagaa gaggaaagac gccgctgcca aaactgaata actacaatga
     1801 ttacaacttt acttcgggtc tgctggacat gtcacacagc agaccctttc tttataatgc
     1861 tgcgcagccg cggccatgca gcgaagtgct tctacttcct tttatgcggc gcagatgagg
     1921 caaggtatga ctagagtcac ctttatgcgg cagcgcaagg tacagactga ccgtggccgc
     1981 atggcttacg cccgccgagc ttgtccacaa actagtagat aacccaggct tcagactgac
     2041 cgtggccgct gcggcgatga aaacttattt atcagaactg acataaaccg cattaaaata
     2101 cgatataata taattaagct aagagattag ctaatgaata aaagcgagcg tgatagtatg
     2161 aacgaagcaa acgagttatc aagattaatg aaagctatga caaaacaaat gcagagcgac
     2221 aacgaccggt atgaagaaaa tccggatgct gatttcaata tcagcaacat cacaattact
     2281 gtcggcgatg agactatacg tcttgttctt ggcggaccgc aggcggcagg tattcattct
     2341 atgatacagc atatagccag cgaaaacatg tattgtgttg atttcgacag agatgaagta
     2401 cacgactact gtagcaaata agtaacacac gaccgcggct cagagccgcg gttacttttg
     2461 cttttatgcg gccacagtca agccgtgtta tactttatgc ggcagcgcaa ggtacagact
     2521 gaccgtggcc gcatggctta cgcccgccga gcttgtccac agactagtag ataaccccgg
     2581 cttcagactg accgtggccg ctgcggtgag taaaacttat ttatcagaac tgacataatc
     2641 cgcgttaaaa tatgatataa ttatattatg aaaggagctg atatatatat gaataaatta
     2701 acaggagcaa gctacagcgc aatacacgac gcaatatgta accagcttga ttatcttgca
     2761 ggtggcgagc agtacgaatt gctgctgcag gcactgaatg aactgaacgc aatatattac
     2821 gacgaggggg tgaataataa tgattagtct gagattgaca gatgtggctg aaagctacag
     2881 ggaaatgcta cataccatcg gcgctgatgc tgacgtttat gcagcaatca tgaataattt
     2941 ctacaagcta tgtgaaacca gcggcgtcgg cgataagact gccgtacagt cgttatatcc
     3001 aggcattact gacgaggagt attatgacgg ctggagcgaa taaacaacac actaccgcgg
     3061 ctattagccg cggtagttgc ttttatgcgg cttcagtcaa gtcaagcaga gctgtacttt
     3121 tatgcggctt cagtcaagtc aagcagagct gtacttttat gcggcggcgc ggcggcgcgg
     3181 actgaccgtg gccgcagcca tacaggggca ataaaaaatt gcaggccaga tgattacccg
     3241 ccgcggtttt taacgccgga ttctctggcc tgcaatttta aatttaggac cggcagggac
     3301 cggtcctatt cattttactg aattacattt tttaacaatt ctacaatttt tccctgtgaa
     3361 atatttccgg aactttcgat tgtatcgatt attttttgaa cgtctgtttt tctgatattc
     3421 acgtcgtcga ccggtttaat taattttcgt ctttcgatta acattctttc aatcttttcg
     3481 gcatttctaa attcgtcgtt gtcgccttct atcgtcgtaa gccaacggct taaagtcttt
     3541 tttgactgta tggtcctaat ggcttttgca agctcgtcat aatcttcaat caaatcgacg
     3601 tcgttttgcg tgtacgttgt tacggtcttt tttgccggtt taatcaggtt ttttgcttct
     3661 tttaatatct gctcctgctg caatatttcg gtcattttat ccgaataatc tgcgcggcct
     3721 ttctgctttt ttaacctgca ttttactgat gcaattcttt tcagctctct gttgacgttt
     3781 tcaaccgttt ttaattcgtt tgcggctttt tcaatctttt ttaaatctgt catatttatc
     3841 ggccctcttt catttatttt tgaatgtata tattattcat tcattaatta tattatatcg
     3901 catattcagc cgttcgtata ccgtataaac agcctaaaat tatgaccgtt ttctgtacgt
     3961 ttatacgggc ctggcgcggc ggcgtataat ccaagcaact catgtaaaat gtataccgca
     4021 taatccaagc aactcatgta aaatgtataa tccaagcaac tcatgtaaaa tatatcacat
     4081 aaaccaagca actcatatta aagtataagt ctgtatatat tccaagcaac aagcctaaaa
     4141 ccagttgtaa aatagtcact gtgctgcctc gctgcataca cagaaaatta aggacaattg
     4201 tagatacgca gctagccgga tatattagtg ccatatgcct taatagtata taatatatat
     4261 atgtgtttat g
//
LOCUS       DASRMS010000163         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_111039, whole genome shotgun sequence.
ACCESSION   DASRMS010000163 DASRMS010000000
VERSION     DASRMS010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111039"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            275..700
                     /locus_tag="VFC74_06980"
     CDS             275..700
                     /locus_tag="VFC74_06980"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HZK25116.1"
                     /translation="MRSNKPRGRLLTAISAVILSSSALAAADLVLNVNDSPDPGYARG
                     IFNYEWRVDNNGPDLAENVEFVHTYPEGAEFVSVAPATGCVDNGITRTLTCQIGTMQN
                     GTSETRDVKIMLPTAQVWSNTGKVTSTTEDLRPGNNSLT"
     gene            791..>2423
                     /locus_tag="VFC74_06985"
     CDS             791..>2423
                     /locus_tag="VFC74_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013508.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF11 domain-containing protein"
                     /protein_id="HZK25117.1"
                     /translation="MVAKNFGPSAIENNQKLKVTFDVPPGAAVTARPTGAGWTCLPAS
                     GYPRASGEITCERTGPLNKDEMADPITVPAVANTSGEVILLASVKGFDANGTNELPEG
                     NLENNESRVTVTTTDGSDVSIIKTQSPAGNAIEIGAEVTYTLTPRHEGGIAPGSTGDG
                     KIIVKDTLSPGLTYIAGSLEAGAGWSCEVVGQEITCERPGPWLGGTFTNMPAIKLKAT
                     ATTVKDTDLSNTAVITIPENDPNPGNNTSTVGKKTTNAADLQMSKTSSLSYPAMLGQE
                     FNYRLRARNIGPLSIPAGDTITITDTLPVGISLEGIEPPAGWTCTSSPNDLPATNATV
                     ECSSTQGLSVGSWTPFTNLRVKALTAGEISNEACVALDSGTVEDKNPSNNCSVIKTIG
                     TQDGQHADVSIVKTADKTTLKAGELLTYTLTVSNAGPALASKVEVRDELNNLLPGNAG
                     GIRTINAPSGTACTPNAPSTGSSKTLVCTIPSLAVGATQDITVTVVPNVAGSVEITRP
                     NTATANSLEVADKNRTNNTSKVDTTVTPLVDLTVTK"
BASE COUNT          711 a          495 c          564 g          653 t
ORIGIN      
        1 aaattgttac atatagagag actctatgtg gcaattcagt gcgcgcgcat atttttcctt
       61 taagtgttta agctttatca attattagct gtgatttgcg caattgtatc tttgattaag
      121 cggcttgggt tgcaataagc aaaacttagc gttgatgttt ttgtattgca gatcatttta
      181 tctatacaca caatatataa atagataaaa atttttgcta ggaacctagc agcatgcaat
      241 acagaaatgc caaataaaat aatggagaat gtttatgcga agtaacaagc cgagggggcg
      301 gttgctcact gcgatcagtg cagtaatctt atcaagcagt gcgctggccg ctgctgacct
      361 agttttgaac gtcaatgaca gtcccgatcc aggttacgca cgcggcatat ttaattatga
      421 atggcgtgtg gataataatg gtcctgatct agctgaaaat gttgaatttg tacataccta
      481 ccctgagggc gccgaattcg tcagtgtggc gcctgctaca ggctgtgttg ataacggcat
      541 tacacgcacg ctgacctgtc aaattggaac catgcaaaat ggtacaagtg agacacgtga
      601 tgtgaagatc atgctgccta cagcacaggt ttggtccaac acaggtaaag tgacaagtac
      661 cacagaagac cttcgtccag gtaataatag cttaacttaa cctactactg ttcgagcagc
      721 cactgatatt gagctaatag cgacttcgtc tgctgataat attgttgctg gtgccgctta
      781 taattatcag ttggttgcta aaaactttgg accaagtgct attgaaaata accaaaaact
      841 taaagtcact tttgatgtac cccctggagc tgctgttact gcacgtccaa cgggagctgg
      901 ctggacttgt ttacctgcta gcgggtatcc gcgagcttct ggcgaaataa cttgtgaacg
      961 taccgggccc ttaaacaaag atgagatggc ggatcctatt acagtccctg cggttgctaa
     1021 tacatcaggt gaagtaatac ttttagcatc agttaaaggt tttgatgcaa acggtacaaa
     1081 cgaattgcca gaaggtaatc ttgaaaataa tgagtcaaga gttactgtca ccacaactga
     1141 cgggtctgat gtttcgatta ttaaaacaca gagtcctgcg ggtaatgcaa ttgaaatcgg
     1201 tgctgaagtt acatacacct taaccccacg tcatgagggt ggtattgcac cgggcagcac
     1261 tggtgatgga aaaataattg ttaaagatac cttaagtcca ggactgactt atattgcagg
     1321 cagtctggaa gcaggtgctg gctggagctg tgaagtcgtt ggtcaagaaa ttacctgtga
     1381 gcgtcctgga ccttggctgg gcggtacttt tactaacatg cctgctatta aattgaaggc
     1441 cacggctaca acagtaaaag acacagattt aagtaataca gctgttataa ctatccctga
     1501 aaatgatcca aacccaggaa ataatacgtc tactgttggt aaaaaaacca ccaatgcagc
     1561 tgacttacag atgtctaaga caagctcgct aagctatccc gctatgcttg gacaagaatt
     1621 caattatcgt ttgagagcta gaaacattgg gcctttaagt atccctgctg gggatacaat
     1681 cactatcact gatactttgc ccgttggtat ttccttagaa ggaattgagc cacctgcagg
     1741 ttggacttgc acaagtagcc caaacgattt gcctgcaacc aatgcaacag tggagtgtag
     1801 ttcgactcaa ggattatcag taggcagctg gacacctttt actaatttaa gagttaaagc
     1861 cttaactgct ggggaaatta gtaatgaggc atgtgtagca ttagatagcg ggacagttga
     1921 ggataagaac ccgagcaata actgttctgt gattaagact atcggaactc aagatggaca
     1981 gcatgctgat gtatctatcg taaaaacagc ggataagacc actttaaaag caggtgagct
     2041 cttaacctac accctgacgg taagtaatgc tggtcctgct ctagcgagca aggttgaggt
     2101 gcgtgatgaa ctgaataacc tgcttccggg taatgcagga gggattcgaa caattaatgc
     2161 gccttccgga acagcctgta ctcctaatgc gccttcgacc ggttcaagca agactttagt
     2221 gtgtacgatt cctagtttgg cagtcggtgc tacgcaagac attaccgtta ctgttgtgcc
     2281 caatgtggcg ggttctgttg agattacccg tcctaataca gcaacggcca actcgcttga
     2341 ggtagctgat aaaaaccgta ctaacaatac atcgaaagtt gacactacgg ttacaccgct
     2401 tgttgatttg acagttacaa aag
//
LOCUS       DASRMS010000164        31417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_517753, whole genome shotgun sequence.
ACCESSION   DASRMS010000164 DASRMS010000000
VERSION     DASRMS010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 31417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31417
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_517753"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..1909)
                     /locus_tag="VFC74_06990"
     CDS             complement(62..1909)
                     /locus_tag="VFC74_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZK25118.1"
                     /translation="MTKHEVLFPGDLRLAVEDGTTLKEIMNDAGINFDFPCGGRGRCG
                     KCLVRITAGANEPLEEEKKKLTEEQINEGIRFACLTKVHSKMSVEFLSDKNIQHKILL
                     SSLDRSAKVEPHITKRYIEVDKATMTDHRTDWQRIRDGLTAQGNISEITNNPVDVLRT
                     IPDLLRNNQHKITAVIYKDEIRSLEAGNTTEKLYGVAFDIGTTTIAGFLMDLYTGKQL
                     SVASTLNPQTQYGADVISRITHVSNDPDGLADLHRAVTQAINKLIGEAAEKAGLERND
                     IYSVSIVGNTCMHHLFLGINPRDVAIAPYVPIVTDSIVVDPQDVQLEMNPGGKVFVLP
                     NIAGFVGADTIGVLLATELDESEDIKLVVDIGTNGEMLLGNKEHILACSTAAGPAFEG
                     AQISSGMRGATGAIDHVTFGEELTYTVIDDVEPLGICGSALLDTVAGLVEFGLLEDTG
                     RIMKPEQVTHPIGQKLKDRLIVHDGSMAFLLEETKARKIMVTQKDIRELQLAKAAMAA
                     GIQLLVKGYGIEVGDIQEVLLAGAFGNYLNPHSACVIGLIPLELEDRVKMVGNAAGAG
                     SQLALLSTSEYQRSGDAARNVQFVELGTNPAFTDVFAEQMLFPDLDELA"
     gene            complement(2430..2505)
                     /locus_tag="VFC74_06995"
     tRNA            complement(2430..2505)
                     /locus_tag="VFC74_06995"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2470..2472),aa:Met,seq:cat)
     gene            2652..3494
                     /locus_tag="VFC74_07000"
     CDS             2652..3494
                     /locus_tag="VFC74_07000"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HZK25119.1"
                     /translation="MRELDVAQISSAVAEMIKEANYVLGDDVYQALQQALEHEESPSG
                     RQVLAQLLKNAELAQREQVPLCQDTGFAVVYLSLGQDVHLFGGDVNEAIQEGIRQGSA
                     DGYLRTSIVADPLQRVNTNDNTPAVIHLTLVPGEEVKLVVAPKGGGSENVSAVKMLKP
                     AEGTAGVIDFVLETVRQAGSNPCPPVIVGVGIGGTLEKVTELAKRALLRKIGQSHPEL
                     FYARLEQELLQRINSLGIGPQGLGGRITALAVHIETYAAHIASLPVAVNINCHAARQL
                     EKTL"
     gene            3517..4113
                     /locus_tag="VFC74_07005"
     CDS             3517..4113
                     /locus_tag="VFC74_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S-containing hydro-lyase"
                     /protein_id="HZK25120.1"
                     /translation="MQVKHLTTPLTTAAVLTLKAGQAVLISGVLYTARDAAHQRLAEL
                     QRTGKTLPFDLEGQLIYYVGPAPAKPGQVIGSAGPTTSGRMDLYTPGLLAQGLKGCIG
                     KGARSQAVKDALVKYQGVYLAATGGAGALLAQTVRQVTVVAYPELGPEAIYRLAVENF
                     PATVINDAYGNDFYVNGQKQYARPISEPGKDENYDPGR"
     gene            4097..5437
                     /locus_tag="VFC74_07010"
     CDS             4097..5437
                     /locus_tag="VFC74_07010"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HZK25121.1"
                     /translation="MTQEDERLARASRPAEEAMRLHPFYRGKIEVGIKCRVQDLQDFA
                     TWYSPGVAAPCKAIAADEKLVYEHTNKGNFVAVVSDGTRVLGLGDIGPEAALPVMEGK
                     ALLFKYLGGVDAFPVCVDTKEPDKLIEFVKMLQPTFGAINLEDISSPKCFYILERLRQ
                     ECQIPVWHDDQQGTATVILAGLINALRYVQKDIHELRIAMIGAGAANICITKLLIQAG
                     ADPGKIYVTDRQGILHRGRASALQPRHQQKYELAVTTNTEGRQGGVAEAMKDCDVAIA
                     LSQPGPGVIQAAWVQAMASEPICFFCANPTPEMWPWQAKEAGAVIVATGRSDFPNQIN
                     NSICFPGIFRGILDVQAAAITDEMCLTAAYTIADLGAENGIDPDQLLPTMDNAEVFIR
                     QAVNVGRKAIELGLARQGLSAAALREKATAAINRARGATQHLMEQGFIAEPPPR"
     gene            5647..7608
                     /gene="acs"
                     /locus_tag="VFC74_07015"
     CDS             5647..7608
                     /gene="acs"
                     /locus_tag="VFC74_07015"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HZK25122.1"
                     /translation="MLKTDVSQQQQVFEPTEEFRQKAYLQSRQEYVDMYDRSIKDSDG
                     FWAEMAEQLHWFKKWEQVREYDFVNAQINWFRGGKLNVSYNCLDRHLTTSRKNKAAII
                     WEGDQPGESKTYTYQQLHREVNRFANVLRKKGVKKGDRVAIYMQMIPQLAIAMLACTR
                     IGAIHTIVFGGFSADALRDRINNCQCKLLITADAGYRAGKVTPLKDTADRALTHCPSI
                     ESVIVYQHTGSEVNMVTGRDSWWDEEMTAENITNFCPPTEMDAEDPLFILYTSGSTGK
                     PKGVLHTTGGYLTYAATTCKYIFDLKEEDTYWCTADIGWITGHTYIVYGPLASGATTL
                     MFEGVPNYPQPDRFWEIVEKHQVNIFYTAPTAIRAMMRDGEKWPNGRDLSSLRLLGTV
                     GEPINPEAWLWYHRVIGKERCPIVDTWWQTETGGILISAIPGAIATKPGSATLPFFGV
                     EPLVIRQDGSTADVNEGGYLVMRGAWPGIMRTVYRDHERFKNTYFTQYPGYYFTGDGA
                     YQDQDGYFWLTGRVDDVINVSGHRLGTADIESALVAHPSVAEAAVVGYPHEIKGEGIY
                     AYAILREGCAASEELKVELIQAVRQAIGPIAKPDCIQICPGLPKTRSGKIMRRILRKI
                     AHNELDTLGDTSTLADPSIVQTLISERVS"
     gene            7762..8535
                     /locus_tag="VFC74_07020"
     CDS             7762..8535
                     /locus_tag="VFC74_07020"
                     /inference="COORDINATES: protein motif:HMM:NF013348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine biosynthesis TtcA family
                     protein"
                     /protein_id="HZK25123.1"
                     /translation="MKLPKWIVRRFWRAIVEFKLLEPGDRVLVGLSGGKDSSFLVTGL
                     KVLQEHAPFHFDLAAVHIDQGFPEIVDLSPLADLCTQLNVPLEIVKTQIYQMAFTEEQ
                     SPCARCAYFRRGFMHNYAEEKQYNKVALAHHLDDAVETFLMSQLFTGQLKTFQPKSYL
                     SRTGVTVIRPLIYFREKEIKQAERLLMLNTVSTPCPYAGHSARAKAKDLLRKLESSND
                     RIFDNLVVAMREGAEKDLWPAPPSKGEFRNLMTDFWQRQ"
     gene            8585..10984
                     /locus_tag="VFC74_07025"
     CDS             8585..10984
                     /locus_tag="VFC74_07025"
                     /inference="COORDINATES: protein motif:HMM:NF025038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25124.1"
                     /translation="MSYRTLKPEECRFFCDPTQFEFETTEAVSPLEGIIGQERAVRAM
                     EFGLLVKRHCYNIFITGPIGTGKNSYAQTLVNEVARSEAVPDDWCYVYNFENPSQPLA
                     LRLPRGQGAHFVKAMQRLVESLKLEIPKAFDADDFERQKADIYRNYQEVRSQLFEELA
                     TLAEEQGFVLKRASSGFVTVPIVDGEEVASEDIANLPEDLKEELEKKTNELQFSSLHV
                     MRRIQAAERVMKEQIKELENRIGLFAVGYLIDELKEKYQEQAEIINYLQAVQKDIMEN
                     LDDFRQAEEESTPFPWLRRKEAAGDKYRVNLVVDNKETIGAPVVVDTNPTYYNLIGRV
                     EYENKLGAMTTDYALIKAGSLLKANGGYLILQARDVLTNAGAWEGLKRVLKTQEACLE
                     NLGEQFSLLAMSSLRPQAIPVQLKVILVGPAELYHLLYHYDDDFRKLFKIKADFDTEM
                     DASRDKMTQMASFVASHTQRAGLRHFDRTGVARLVEYSSRQAEHQQKLSTRFNEIMEI
                     ISEADAWASIKGDDLVRAEHVKQAIEEKIYRSNTYEQKLQKLLAEGTILLDLKGEKVG
                     QVNGLSVLDSGDYAFGRATRITAVTYLGREGIINIERETKLSGRIHDKGLLTLSGYLA
                     AKFAQKAPLSLSASIAFEQMYGGIDGDSASSTELYALLSSLAELPLRQDIAVTGSVNQ
                     HGEIQPIGGVTEKVEGFFKACKLLGLTGKQGVMIPVQNIINLNLNEEVITAMGEGKFH
                     LYAVSTIAEGIEVLTGVAAGHLDKEGDYPTETVYGRVAAKLADYNSLLQQAKSDNDEE
                     E"
     gene            11071..12141
                     /gene="mnmH"
                     /locus_tag="VFC74_07030"
     CDS             11071..12141
                     /gene="mnmH"
                     /locus_tag="VFC74_07030"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HZK25125.1"
                     /translation="MKRQLTVEQLLAEREHFYLADVRSPKEYAEAHIPGAANIPLLEN
                     EERALVGITYKDEGTDRAKQVGLSLVAPRLPQMIEKIRRQAKGKKVVIYCWRGGTRSR
                     SICAIMEALNYPVWQLQGGYKAFRRYIHSYLSQATVTMPVFVLNGLTGVGKTHIIKIL
                     QQQGYPALDLEGMANHRGSAFGAVGLGQARSQKDFEALLALFLLEHHAASYLVIEGEG
                     KRIGSATLPDFLFQAMEEGQHLLLEADVAVRVGRIVEEYQGRADHLGDLAEAVKTLSR
                     KLGKAKSEELASLIRQGELETVVKTLCTDYYDHYYSDSRQGREKYLAVVSVNELNKGA
                     AEILTIINDYLAAQPGFVGREE"
     gene            12148..12606
                     /gene="trmL"
                     /locus_tag="VFC74_07035"
     CDS             12148..12606
                     /gene="trmL"
                     /locus_tag="VFC74_07035"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008175 - tRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HZK25126.1"
                     /translation="MHIVLVEPEIPPNTGNISRTCAVTNTTLHLVKPLGFSLDERHLK
                     RAGLDYWQHLQLMMHDNLASFLPTISAQSCWFLTTKGQRLYTDVVYQPSDYLFFGKET
                     AGLPAWLLAKYPERCLRVPMGPQLRSLNLSNTVAIVLYEALRQQDFPALI"
     gene            12676..12951
                     /locus_tag="VFC74_07040"
     CDS             12676..12951
                     /locus_tag="VFC74_07040"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZK25127.1"
                     /translation="MILLRGPKKDATLRRLKTAGGHLKAVERMVEDDKYCADVLLQLQ
                     AVIASLEKVSNIILENHLETCLRAAMRRGEEEEVIDELKTFMKYKIK"
     gene            13081..13290
                     /locus_tag="VFC74_07045"
     CDS             13081..13290
                     /locus_tag="VFC74_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HZK25128.1"
                     /translation="MSESLTLSISGMTCNHCKLRVEKALKTLTGVESVAVDVQAGRAE
                     VQYDPAQVSGESLKTVVTEAGYEVK"
     gene            13389..13634
                     /locus_tag="VFC74_07050"
     CDS             13389..13634
                     /locus_tag="VFC74_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25129.1"
                     /translation="MSAVKLSIISCLLMLGMLMLPSLTGAGMGSMVTRLWLAFGILVF
                     TGNYLAYQQEAAQQAAKDNQRTRQGEHPLRQEVRSLS"
     gene            13838..17269
                     /locus_tag="VFC74_07055"
     CDS             13838..17269
                     /locus_tag="VFC74_07055"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011721387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HZK25130.1"
                     /translation="MKSKSFVHLHTHTEYSLLDGACKIKEVVAEAVKLGMPALAITDH
                     GVMYGVIDFYKAAKKAGIKPIIGCEIYVAPRMRYDRVAHRDSKQHHLVLLARNNEGYQ
                     NLMQLVTRGFSEGFYYKPRVDHQLLARYNSGLIALSACLAGEIPMALLENRHEDARQL
                     VQFYQETFGSENFYLELQDHGLAEQKALNPQLIQLARDTNTPLVVTNDLHYIRKEDAA
                     PHDVLLCIQTGKTLEDTNRMRFSTDEFYLKSPQAMAALFPELPEVVTNSLEIADRCNV
                     DFEFGNTHLPVYEIPADYQDDFSYLKELCTAGAKKRYGDPLTAEVEERLNYELQVIHQ
                     MGYDSYFLIVWDFVNYAHQHEIFVGPGRGSAAGSLIAYSLGITNIDPLKYNLLFERFL
                     NPERVTMPDIDIDFCYEKRERVIEYVTNKYGADSVSQIITFGTMAARAAVRDVGRVLN
                     IPYAEVDKISKLIPAELNITISDALDKAPDLKELYTTDGRIHQLLDLSMAVEGLPRHA
                     SMHAAGVVISGKPLVEYVPLAKSGDSMVAQFSMTTLEELGLLKMDFLGLRTLTIMGEA
                     VRLIKQSTGREIDLNKLPLDDEKTYTMLAQGDTSGVFQLESSGMRSVLRELKPTVFED
                     IIAVVALYRPGPMDQIPTFIQSKHGDIPINYLHPVLEPILEETYGVMVYQEQIMQVAS
                     AVAGFSLGEADLLRRAISKKKLEVLNQQRQLFVKGCVSKGHSKELANELYDLIVKFAS
                     YGFNKSHAAAYALVAYQTAYLKVNYPTQYMAAQLTGVMSTTDKVAGYIFDCKKMGISV
                     LPPCVNSSGKNFTVTAEREIRYGLAAVKNVGLGAIESIEAARAEKGAFTSLRDFCARI
                     DLRSCNKKVMENLIKSGAFDWLQANRNQISAVIDETVMAAQTLSREQQNGQISMFDLV
                     EDEDEAEWGQLHDDFPALPEPTPMERLTMEKEALGLYISGHPLEEYTEVLKLRSEIVP
                     LHELADLPDEQSVVTVGMISGFKPIITKKGKAMSFLTLEDLTSETEVVVFPEVHEKAK
                     QLLQNDQVVLVQGKVSHREEEEAKILASAIALLPQVAEEVVIRCRPDGSLSQLLSLKD
                     LLQGQQGILPVYLLFPVVQKKVLLSSDYWLPENAPQIVEIQNLFGEDAVTVQKVG"
     gene            17328..18287
                     /gene="pfkA"
                     /locus_tag="VFC74_07060"
     CDS             17328..18287
                     /gene="pfkA"
                     /locus_tag="VFC74_07060"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HZK25131.1"
                     /translation="MRKIGVLTSGGDAPGMNAAIRAVVRKSIYHGLEAFGFRRGYYGL
                     MNGEYEILQLGSVADIIHRGGTMLLTARAPEFLETGGRKKAISVIHESGIDALIVIGG
                     DGSLRGAAELEKLGVSTVGIPGTIDNDIACTDYTIGFDTAVSTVTEAINKVRDTATSH
                     ERIYVIEVMGRSAGYIALAAGLSGGAESILVPEIPFALNEVAERLQRGVKRGKLHSII
                     LVAEGASSAIDIAEQLKALTSMDIRVTILGHVQRGGTPTSFDRILASRLGAAAVDLLR
                     SGCHGLMVGLKDGEIKSTPYEEALNQQKQFPHDLYNLATILAI"
     gene            18312..20060
                     /gene="pyk"
                     /locus_tag="VFC74_07065"
     CDS             18312..20060
                     /gene="pyk"
                     /locus_tag="VFC74_07065"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013841399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HZK25132.1"
                     /translation="MRRTKIVCTLGPSSNQVAVLTSLIQAGMNVARLNFSHGNHAEHA
                     ATVSAVRQAAKEVGNNLAILLDTKGPEIRIGAFIADKITLQAGESFKLTTNQVDGTQD
                     QVYVNYPGIINDVQPGMQVLLDDGLIVLEIMDITSTELNCRVITGGELANHKGVNVPA
                     ARLQMPALSASDREDIIFAIKFDLDFIAASFTRKADDILAIRAILEEFRSDIPIIAKI
                     ESEEGVAEIDKILSVADGVMVARGDLGVEVPAEDVPLIQKRLIKKCNAAGKPVIIATQ
                     MLDSMIRNPRPTRAEASDVANAIFDGADAVMLSGETAVGKYPVEAVQTMARIAARAET
                     ALEYESNLETIFAPPEQSVTDAIAYATCHAAQELGAKAIITSTQSGFTARNVAKYKPK
                     ARIVAVTPRKAVARRLNLTWGVFPLLCRPATNTDEMFATAIEAALGSEYIKNGDLVVI
                     TAGVPVGVSGTTNLLRIHTVGEIILRGTGLGKKAVTGPVKVALTAAEAANLEEGQILL
                     ARATDKAYVPYLNKAAGLLVEEGGLTSHAAVLALHLGLPVIVGAADAAAIVKDGEIVT
                     LDTARGLVYRGKATTF"
     gene            20071..20505
                     /locus_tag="VFC74_07070"
     CDS             20071..20505
                     /locus_tag="VFC74_07070"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HZK25133.1"
                     /translation="MAMKNGEALIRVRYEETDQMGMVYYANYFIWMEIGRNEYFRLLG
                     MSYSEVEKNGILLPVTKAFCQYQSPAHYDDLLRLVTSVASLQDVRIVFKYEIFNASTD
                     NLLAIGETEHAFVNPQGRPVVLKKQNPFFWKRMLEATGENME"
     gene            20511..20903
                     /locus_tag="VFC74_07075"
     CDS             20511..20903
                     /locus_tag="VFC74_07075"
                     /inference="COORDINATES: protein motif:HMM:NF008528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FxsA family protein"
                     /protein_id="HZK25134.1"
                     /translation="MLGRLLLLMIIVPILEIFLLIEVGQQIGVLPTVTIVLLTGLLGI
                     MLARTQGFYVLTRIVNSLRKGELPGDDILNGLLVLIGASFLLTPGLITDTAGFILLIP
                     RTRRTVKVWLYYKLTKALKDGTLRIFRR"
     gene            complement(20970..22295)
                     /locus_tag="VFC74_07080"
     CDS             complement(20970..22295)
                     /locus_tag="VFC74_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HZK25135.1"
                     /translation="MATIVPICGVRYNTAQVGQMADLVTPPYDVINEQEQQRYYEKNP
                     YNVIRLEYGETRINDSAEDNRYTRAAGFFKQWLQQNILQHEEKPAVFLYEQEFNTKGK
                     SLTRTGLISGVKLEEYEKGIILPHEETLPQAKADRLQLLRHCRANFSPVFSVYDDPAF
                     TVENIAATYKKNKPLVTFSGENDEVHRLWVINDQQALTKMQEFFSNQSIYIADGHHRY
                     ETALHFQREMRAQGDERFGFCLMALVNLHDPGLVIFPTHRLVKGLPQLVQDTLLTQLS
                     RSFIVKTIDFPRTQRQSLLAAELTKLAALLPTYNAILLYLGGEKLYQLTLKRDSNNAT
                     LVKRYGDYSAAWRSLDVTVLHTLILEELLGIDSAARAQGTQLQYTRDATEALNLVDHG
                     TYQAAFLLNPTPVQEVTAVATAGDKMPQKTTYFYPKLPTGLVINDFSVE"
     gene            complement(22371..22715)
                     /locus_tag="VFC74_07085"
     CDS             complement(22371..22715)
                     /locus_tag="VFC74_07085"
                     /inference="COORDINATES: protein motif:HMM:NF019401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HZK25136.1"
                     /translation="MNLHALESFLTTILTLGSHLLEIFGAIIILSAAVKTFFYFLTTG
                     RCDRSARLNFARYLVFGLDFKLAGEILRTVIVRSMSEVVILASVVALRFILNLILHWE
                     IHQEQKDQASEE"
     gene            23102..23491
                     /locus_tag="VFC74_07090"
     CDS             23102..23491
                     /locus_tag="VFC74_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25137.1"
                     /translation="MRLQGQAVTIARGDVAKALAQRTIYSARRVVPEFNDVASSTAVN
                     RCAYLLRSTFGEPSYVAHRPLDGPVEVWVVSLKNGNGIISFELWQNSEMPRYYIFTDK
                     PTPIVAKLLRRLGRYLRAPNVYVVPNK"
     gene            23678..24343
                     /locus_tag="VFC74_07095"
     CDS             23678..24343
                     /locus_tag="VFC74_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS and ACT domain-containing protein"
                     /protein_id="HZK25138.1"
                     /translation="MLVKDRMSPHVYTITPDTPIPDALQLMEEKKIRRLPVIDKDRLV
                     GIVTLLDLVRASPSPATSLSVWELNYLLAKLPVKDVMAKQVYCVTPETTIDKAASLMR
                     EHSIGGLPVLDGEQIVGMITETDIFTAFLEMLGVHRGGLRLTLELTERHGALADIALL
                     FRDFGVNIVSAVAFISDEDQELAHSVWRLEGCDDVEGLIKYLQEKEYKVIHYSLNGAG
                     TEV"
     gene            complement(24410..26239)
                     /gene="typA"
                     /locus_tag="VFC74_07100"
     CDS             complement(24410..26239)
                     /gene="typA"
                     /locus_tag="VFC74_07100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HZK25139.1"
                     /translation="MQIRNIAIIAHVDHGKTTLVDGMLRQSGIFRSNEAVVERVMDSN
                     DLERERGITILAKNTAVEYRGVKINIVDTPGHADFGGEVERALSMVDGVLLIVDAFEG
                     PMPQTRFVLRKALELGLLPIVVVNKIDRSDARPLQVVDMVFDLFVELGANDEQLDFPV
                     IYASARTGMASLEPDENSSSLEPLFEVIVSHVPAPASDEHGPFQMLVANLTHDNYVGR
                     YAIGRILRGTIAAGEAVALLHADGSVEQARVNKVFVFSGLKKMETDNAMTGDIIALSG
                     LDNVNIGETICHPDHLEQLPVLTVDEPTLTMYFVVNNSPFAGREGQFVTSRKLRERLF
                     HELETNVSLKVEETDSPDSFLVAGRGELHLSILIENMRREGYELQVSKPEAIFKKIDG
                     KLHEPIEHLVLDMSEEYMGAIMENLSQRKAEMVDMTPTEPGQIRLEFRIPARGLIGFR
                     SEYLSETRGNGIMNHTFSSYEPHKGEISSRFQGALIAWEDGEATAYGLHAAEERGTLF
                     VVPGAHVYEGMIVGQNNREVDLEVNICKKKHLTNMRASTSEETVRLKEPRNLSLEEAI
                     EFIADDELVEITPQSFRLRKKLLKKQERAQFNKTRAMRRAQTS"
     gene            26399..28342
                     /locus_tag="VFC74_07105"
     CDS             26399..28342
                     /locus_tag="VFC74_07105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HZK25140.1"
                     /translation="MSVAAVHHVKKYFGAELILDDASLQLHPRERVGLVGPNGAGKTT
                     LLNIIAGEVIADEGTITLARGSSLGYLKQGSKSSASGTMEVELRRAFAEIDALAEQLN
                     SLEQQMAARSSQSDKQLEEIMSTYGELRHRFEDAGGYLMDARLRAVVNGLGFQMQDLP
                     RTIATFSGGEQTRIRLARLLLEEPEVLLLDEPTNHLDIEAVEWLEKYLNDWHGTILIV
                     SHDRYFLDRVATRILALEHGQLKSYQGNYSAYQRQREQERITQEKAYAKQQEYIEKET
                     TYIRTLGTGEREKRQAKSRQKRLAKLQIMDKPQQARAMALDFGFSGRSGEIAVRMEGV
                     AKYFADQAVFSQVDFVLRWGERVALVGPNGSGKSTLLKLIAGELQPDVGQVWVGPSVK
                     LVYFDQHQQIIASDKTPLEEIMDAASMTITEARSYLGRFLFIGDDVFKRNADLSGGEK
                     SRLALAKLGLDEGNFLVLDEPTNHLDITGVEELEEAICSFPGTLIVVSHDRYFVSRTT
                     EKVLDVAKGCVTYYPVPYDEYVQEREKKAVIEIDPAESKKIQRHAEQRRQREAELQQR
                     RELRKLTEEVEQTETEISSLEEKLAVLEAELLEPQVYSDYQLAAEKGQELAQVKARLQ
                     LLYRQWELQTEQVEKFKGKIILG"
     gene            28568..30556
                     /gene="tkt"
                     /locus_tag="VFC74_07110"
     CDS             28568..30556
                     /gene="tkt"
                     /locus_tag="VFC74_07110"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HZK25141.1"
                     /translation="MNSIEERAVNAIRFLAVDAIEKANSGHPGLPMGGAPMAYILWSR
                     FMRHSPKHPSWENRDRFVLSAGHGSMLLYALLHLFGYGLSVADLQQFRQWGSKTPGHP
                     EYGHTVGVETTTGPLGQGFANAVGMAIAERRLAAEFNKPEFEVVNHYTYVYTGDGCLM
                     EGVSAEAASLAGHLKLGRLICLYDDNEITIDGSTKVAFTEDVAQRFAAYGWHVQKIAD
                     GTELSAIAEAIAKAQQESARPSLIMVRTAIGYGSPNKQGLSAAHGAPLGSDEVNLTKE
                     NLGWPLEPAFFVPDEVREHFAALTTQQEAAYTAWHDLFADYRQRFPEDAKRWDDWHSL
                     DVPAALAEEEELWQFTKPLATRAASGQVMQIIAKHLPNLLGGSADLNASTKTYLKAFS
                     DFQAENYCGNNLFFGVREHAMGAIVNGLALYGGLRPYSSTFFVFVDYMKPTLRLAALM
                     GLPVVYVFTHDSIGVGEDGPTHQPVEQLANLRSIPNLHVLRPADGRETAAAWLSAVKR
                     VDGPTALVLSRQKLPQLSGTGLEAQKGAYILSPEKGQAPAAILLASGSEVHLALGAQQ
                     ELWKKGIDVRVVSMMSWELFRAQSPVYREEVLPKAIKKRLAIEAGHTMGWHSFIGDEG
                     EMIAIDHFGASAPGEILMEKFGFTVANIIEKVTYLLQK"
     gene            complement(30633..>31417)
                     /locus_tag="VFC74_07115"
     CDS             complement(30633..>31417)
                     /locus_tag="VFC74_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003405400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HZK25142.1"
                     /translation="ELEKLLRDDRETYEKFFQTFGRQLKFGVYSEFGSHKEDLQDLLL
                     FYSSHEKKLVTLAEYVGRMKSEQQYIYYAAGESVARIEKMPQTELLQEKGFEILYFSE
                     DIDEFAIKMLAAYQDKEFKSISNADLGIEDETPATAEPAAEQKLFDFMQDVLSEQVSS
                     VRASKRLKNHPVCLANAGEVSIEMEKILNAMPNNQNIKADKVLEINTNHEVFKSLQEA
                     YEQDQDKLKLYTNLLFNQALLIEGINIEDPVEFTNNICSLMK"
BASE COUNT         8783 a         6420 c         7541 g         8673 t
ORIGIN      
        1 atattattta ctggtacaaa aaaagctgcc tcgctaggca gcttttttgc ttgaatgtga
       61 tttaagctaa ctcatctaaa tctggaaata gcatttgttc tgcgaagaca tcagtgaagg
      121 ccggatttgt accaagttcc acaaattgta cattgcgcgc agcatcacct gaacgttggt
      181 attcacttgt tgagagcaaa gctaattgtg accctgctcc tgcggcgtta cctaccattt
      241 taactctatc ttctaattcg agaggaatca gcccgataac acaggcacta tgagggttga
      301 ggtagttacc aaatgctccg gcgaggagga cttcttgaat atcgcctact tcgatgccat
      361 aaccttttac taaaagctga ataccggcag ccatagcagc ttttgctaat tgaagttcac
      421 ggatatcttt ttgcgttacc atgatctttc tggcttttgt ttcttctagg agaaaagcca
      481 tcgagccatc gtgcacaatt aaacgatcct tgagtttctg cccaatagga tgggtaactt
      541 gttctggttt cataatacga ccggtatctt ccagcaggcc aaactcaact aaacctgcta
      601 ctgtatccag taaagcagaa ccgcagatgc ctaacggttc tacatcatca atgaccgtat
      661 aggttaattc ttcaccaaag gtaacatggt caatcgcgcc agtagcacca cgcataccag
      721 aactaatttg cgctccttca aaagcaggtc cagcagccgt ggagcaggct aggatatgct
      781 ccttattgcc aagcagcatt tcaccattgg tgccgatatc gacaactagt ttaatatctt
      841 cactctcatc aagttccgtt gccaatagca cgccaattgt atctgcacca acaaagcctg
      901 caatattagg tagaacaaaa actttaccac cagggttcat ttctagctgc acatcttgag
      961 gatcaactac gatactatca gtaacaatcg gtacgtaagg agcaatggcg acatcccgag
     1021 ggttaatgcc taagaaaaga tgatgcatgc aggtgttacc cacgatggac acggaataaa
     1081 tatcattgcg ctctaaacca gctttttcag cagcctcacc aattaatttg ttaattgcct
     1141 gggttactgc acgatgcaag tcggctaggc catcaggatc attacttaca tgggtgatac
     1201 gtgaaataac atctgccccg tactgtgttt gcgggtttaa tgttgaagcc acacttaatt
     1261 gtttgccagt ataaaggtcc atgagaaaac cggcgatagt agttgttcca atatcgaaag
     1321 caacaccgta taatttttct gtggtattgc cagcctctaa actgcggatt tcatctttat
     1381 agatgacagc agtaatttta tgctgattat tccgtaagag atcagggata gttctaagta
     1441 catcaacagg attgttagta atctcactaa tattgccttg agcggtcaga ccatctctaa
     1501 tacgctgcca gtctgtacga tggtcggtca tcgtggcttt gtctacttcg atatagcgtt
     1561 tagtgatatg cggctctact ttcgctgatc tgtcgagcga agaaagtaag atcttgtgct
     1621 gaatattctt atctgataaa aattctacac tcatcttact atgaactttg gttaaacagg
     1681 caaagcgaat gccttcatta atttgttctt cagttaactt tttcttttct tcttccaggg
     1741 gttcattagc accagcagtt atccgaacga ggcactttcc gcagcggcct ctaccgccac
     1801 aaggaaagtc gaaattgata cctgcatcat tcatgatttc tttaagtgta gttccgtctt
     1861 caactgctaa tcttaagtct cctggaaata atacttcgtg ttttgtcata cccatcacct
     1921 ttcgcattta ttctgcataa cgctggtaat acttaatcta gtaacagatt agttctcata
     1981 ctataataat aacaattttc gctaaattct tcaaccataa ggcaacattg ggaaaatatt
     2041 aaccaccaag ctaggcaggt agttaagaac aatctaccac atgccagagg taaaatactt
     2101 acacaagaat taagaactca atcttggcca gataccatgg ggcagttagc ttcgaggtca
     2161 cgctagagag taattcccag gcacgattag cagaacgcct gcttatattt tttagttaaa
     2221 acttctaatt attcacaaag tatctactca gccgtagact ttatataaca aaaaacgcat
     2281 cctataatta gaatgcgttt tttgcttgag catgattttt catattatcc tcctatgtaa
     2341 acaaaggaat ctagcccaca atcttgtcca aagccaaaaa aaaagctaat attgacactc
     2401 accgtatcaa aattagcgtt ttctctatat ggttgcgggg gcaggatttg aacctgcgac
     2461 ctccgggtta tgagcccgac gagctgccag ctgctccacc ccgcgtcaac gagattaatt
     2521 ataacaaaag atagaatgct tgtcaaatgg aataatgtta caacctcggc attaataaca
     2581 tctttgcatc tgcttgtgct ataatcatat aaaaagttaa tacaattgcc aggaagtggg
     2641 gtttgaaaaa tatgcgcgaa cttgatgtgg cacaaataag ttcagctgta gcggaaatga
     2701 tcaaagaggc aaactatgtt ttaggtgacg atgtttatca ggctttgcag caagccttgg
     2761 agcatgaaga gtcgcctagt gggcgtcagg tgttagcaca attgctaaaa aacgctgagc
     2821 ttgcccaaag agagcaggtg ccgctgtgtc aagataccgg ctttgctgta gtatatttgt
     2881 cattaggaca agatgtgcat ctctttggtg gtgatgtcaa cgaagccatt caagagggca
     2941 tacgtcaagg ctctgctgac ggttatttgc gcacatcaat cgttgctgat cctctgcagc
     3001 gggtaaatac taatgataat acacctgcgg tcattcatct gaccttagtg ccaggagagg
     3061 aagtaaagct agtcgtcgca ccaaaaggtg ggggcagtga aaatgtcagc gctgtaaaga
     3121 tgctcaaacc tgctgagggt acagctggtg tgatagattt tgttcttgag acggtacgac
     3181 aggcaggcag caatccctgc ccacctgtta ttgtcggtgt tggtatagga ggtacgttgg
     3241 aaaaggttac cgaattagcc aaaagagctt tgctgcgaaa aattggtcag tcccatccag
     3301 agttgtttta tgcaagattg gagcaagaac tattgcagcg tattaacagc ctaggcattg
     3361 ggccacaggg attgggcggc agaattacag ctttggctgt gcatattgaa acatatgcag
     3421 ctcatattgc cagcctgcct gtggctgtga atattaattg ccatgctgcc cgccaactag
     3481 aaaaaacttt gtagaatgca gggaggtgaa ctatcgatgc aagttaaaca tcttactacg
     3541 ccgctgacca cggccgcagt gttaacacta aaagctggtc aagcagtgct aatttccggt
     3601 gttttatata cagcacgcga tgccgcccat caacggctgg ctgaattgca gagaactggt
     3661 aaaactctgc cctttgatct tgaaggtcag ctgatatatt atgttggtcc ggctcccgca
     3721 aaaccagggc aggtgattgg ttcagctggt cccacaacca gtggtcggat ggatctttat
     3781 accccggggc tcttagcaca gggtcttaaa ggttgtatcg ggaaaggtgc acgcagccag
     3841 gcagtaaaag atgctttggt aaagtatcaa ggtgtttact tggctgctac agggggtgcg
     3901 ggagcccttt tagcccaaac tgtccggcag gtgacagtgg tggcgtatcc tgaattaggg
     3961 ccagaggcta tctaccgttt agctgtggaa aatttcccgg caacagtaat taatgatgcc
     4021 tatggtaatg atttttatgt gaatggtcaa aagcagtatg cacggccgat tagtgaacca
     4081 ggaaaggatg aaaactatga cccaggaaga tgaacgcctt gccagggcgt caagaccggc
     4141 cgaagaagct atgcggcttc atccctttta ccgggggaaa attgaggttg gcataaaatg
     4201 cagagtacag gacttgcagg actttgctac ttggtattcg cctggtgtcg ctgcgccctg
     4261 taaagccatc gctgcagatg aaaagttagt ctatgaacat accaataaag gtaactttgt
     4321 ggcggttgtc tcggatggta cccgtgtctt gggtcttggt gatataggtc ctgaagcggc
     4381 actgccggtg atggaaggca aggcactgct atttaaatat ttaggtggtg ttgatgcttt
     4441 ccctgtttgt gtagatacaa aagaaccaga taaattgatt gagtttgtta aaatgctgca
     4501 gccgacattt ggggccatta atctggagga tatttccagc cctaaatgtt tttatattct
     4561 tgagcgcttg cgtcaagagt gtcagattcc tgtctggcat gatgaccagc agggaacagc
     4621 aactgtgatt ttagctggac ttattaatgc cctccgctat gtgcaaaaag atatccacga
     4681 gcttcgcatt gcgatgatcg gcgccggtgc agctaatatc tgcattacga aactgcttat
     4741 ccaagccgga gctgatcctg gcaagattta tgtgactgat cgccaaggaa tattacatcg
     4801 aggacgtgct tctgctctgc agcctaggca ccagcaaaaa tatgaattag ctgtgactac
     4861 taatactgaa ggccgccaag gtggggtagc agaagcgatg aaagactgtg atgtggcgat
     4921 tgctctctca cagcctggtc cgggggtgat ccaggccgcg tgggtgcaag caatggcctc
     4981 tgagcccatt tgttttttct gtgctaatcc taccccagaa atgtggccgt ggcaggcaaa
     5041 agaagctgga gctgtgattg tcgctaccgg acggtccgac ttccccaatc agataaataa
     5101 ttccatttgc ttcccgggta ttttccgtgg tattttggat gtgcaggctg ctgccatcac
     5161 ggacgagatg tgtctgacag ctgcttacac tattgctgat ttaggggcag agaatggtat
     5221 cgatcccgac cagctcttgc ccacaatgga taatgccgaa gtttttatcc gtcaagccgt
     5281 aaatgtgggc aggaaagcta ttgaactagg gttagcacgc caaggccttt ctgctgccgc
     5341 tttacgtgag aaggcgacag cggcgataaa tcgggcgcgt ggtgcgaccc agcacttaat
     5401 ggagcaaggt tttattgctg aaccaccccc acgttaaagg caaattgtaa tacttacgta
     5461 attcacaaaa agtagtcatc tattatacta ttattgtaag gacgtaagct gaagcagaga
     5521 ttcccgctaa aaaaatgtaa cgacttattg cagagtaggc accggatcag tataatgtat
     5581 aatagcggga gtattattta taattagttt ctggtcacta aaatattatg aatggggagg
     5641 ttgaacatgt tgaaaactga tgtttcacaa caacagcagg tttttgaacc tacagaagaa
     5701 tttcgtcaaa aagcatattt acagagcagg caagaatatg ttgacatgta cgaccgctcc
     5761 attaaggact cggatggttt ttgggctgaa atggcagaac agcttcactg gtttaaaaag
     5821 tgggagcaag ttcgggagta cgactttgtt aatgcccaaa ttaactggtt ccggggcgga
     5881 aaactaaacg tctcgtataa ctgtttagac cggcacctaa caacgtcgcg caaaaataag
     5941 gccgctatta tctgggaggg cgatcagcca ggagaaagta agacctatac ctaccagcag
     6001 ctgcacagag aagtaaatcg ttttgctaat gttttgcgta aaaagggcgt aaagaaaggc
     6061 gaccgcgtag ctatttatat gcaaatgatt ccgcagctgg ccatcgctat gttagcttgc
     6121 acacgtattg gtgccattca cactattgtt ttcggtggtt ttagtgcaga tgccttacgt
     6181 gacagaatca acaactgcca gtgcaagctg ctgatcacag cagatgctgg ctaccgtgct
     6241 ggtaaggtca cacctttgaa ggatacagct gaccgggcgc taacacattg ccccagcatt
     6301 gaaagcgtga tagtttatca gcatacagga agcgaagtta acatggtaac cggacgagac
     6361 agctggtggg atgaggaaat gacagccgag aatattacta atttctgtcc acccacggaa
     6421 atggatgcag aagatccttt gtttattctt tataccagcg gtagtaccgg taaaccaaaa
     6481 ggtgtcttgc acactacagg cggatattta acctacgctg ctacgacgtg taaatatatt
     6541 tttgatctta aagaagagga cacctactgg tgtacagccg atattggctg gattactgga
     6601 cacacttata ttgtttacgg ccccttggcc tcaggcgcca ccacgttaat gtttgagggt
     6661 gtacccaact atcctcagcc agatcgcttc tgggaaatag tagaaaagca tcaagttaat
     6721 atcttctata ctgcgcctac tgctatccgt gctatgatgc gagatggaga aaaatggcct
     6781 aatggccgtg atttaagcag tcttcgttta cttggtaccg ttggcgagcc tattaatccg
     6841 gaagcttggt tatggtatca tcgcgtaatt ggtaaagaac gctgcccgat tgttgatacc
     6901 tggtggcaaa ccgaaacggg tggtattttg atctcggcta ttcctggcgc cattgccaca
     6961 aagccaggtt ctgctacctt accattcttt ggtgttgaac cattagtaat tcgacaagac
     7021 ggtagtacgg cagatgtgaa tgaaggcggt tatctggtta tgcgcggcgc ttggccaggg
     7081 ataatgcgca ctgtttaccg tgatcatgag cgctttaaga atacctactt tacccaatac
     7141 cctggttact atttcaccgg tgatggtgca tatcaagatc aagatggata cttctggcta
     7201 acaggtcgcg ttgatgatgt gattaatgtt tcaggccatc gccttggtac agccgatatt
     7261 gaaagtgcct tagtcgccca tccctctgtc gctgaagctg ctgtcgtcgg ctaccctcat
     7321 gaaatcaaag gtgaaggaat ttatgcttac gccatcctgc gggaggggtg tgcggcaagt
     7381 gaggaattaa aggttgagtt aattcaagca gtacgtcagg ccattggccc gattgccaaa
     7441 ccggattgta ttcaaatctg ccccggctta cctaaaactc gtagtggtaa aatcatgcgg
     7501 cgaattttac gcaagatagc ccataatgag ttagatactt taggtgatac ctcaaccctg
     7561 gccgatccct caattgtgca aacattaatt tctgaacgtg tcagctaaat aataaactag
     7621 tgcccgtggc taagccgcgg gccttgtatt ttcggctaaa aattaagttc tgcctccttg
     7681 tgatttcctt aaactctgtt aatggaattt tgttatggaa gatgataaga taaggccaga
     7741 ccaaaaatgt ggaggcaaaa catgaagcta cctaaatgga tcgtacgccg cttctggcga
     7801 gcgattgtag agtttaaact gctggaacca ggtgatcgtg ttttagtagg gctctcagga
     7861 ggtaaagatt cctctttctt agtaaccggc ttaaaagtat tgcaggagca tgctcctttt
     7921 cactttgatt tagccgcagt gcatattgat cagggtttcc ccgagatagt tgatctctct
     7981 ccactggcag acctatgtac acagctcaat gtgccactgg aaattgtcaa aacacagatt
     8041 tatcaaatgg catttacaga agagcaaagc ccttgcgccc gttgtgctta ttttcgacgt
     8101 ggttttatgc ataattatgc cgaggaaaag caatacaata aagtagcact agcgcatcac
     8161 ttagatgatg cagtagagac atttctcatg tcacaattat ttacaggaca gttaaaaacc
     8221 tttcaaccca aaagttattt aagccggacc ggtgttacgg tcattcgacc tttaatatac
     8281 tttagggaaa aagaaattaa gcaggctgag cgtcttttga tgctaaatac agtgagtaca
     8341 ccttgtcctt atgccggcca ttcagcacgg gctaaggcca aggatttgct acgcaaacta
     8401 gagagcagta atgatcgtat ttttgataat ttagttgttg ctatgcgtga aggcgcagaa
     8461 aaagatttgt ggccggcgcc gccgagcaaa ggggagtttc gcaatttaat gactgatttt
     8521 tggcagcggc agtagtcggc agcaggaaat atacaattat cgttgcgatg aagggggaaa
     8581 aaaaatgagc tatcggacat tgaagccaga ggaatgtcga ttcttttgtg acccaacaca
     8641 atttgaattt gaaacaacag aggctgtgtc gcccttagaa ggtattatcg gacaagaacg
     8701 tgcggttcgg gccatggagt ttggcttgct ggtaaaaaga cattgttata atatttttat
     8761 caccggtcct ataggcacag gcaagaattc atatgctcag actttagtca atgaagtggc
     8821 aaggtcagaa gcggtgccgg atgactggtg ctacgtatat aactttgaga accccagcca
     8881 gccgctcgcc ttacgtctgc ccagggggca gggtgcacac ttcgtcaagg ctatgcagag
     8941 actggtggag tctttaaaac tggaaattcc caaggctttt gatgctgatg attttgaacg
     9001 gcaaaaggcg gatatctacc gtaactatca ggaagttcgc tcacaactat tcgaagagtt
     9061 ggcaactttg gcagaggaac agggttttgt ccttaagcga gctagttctg gctttgttac
     9121 tgtgcccatt gtggatggag aagaagttgc ctcagaagat attgctaatt tgcctgagga
     9181 tttaaaagag gagctagaaa aaaagaccaa tgaactccag tttagttctc tgcatgtcat
     9241 gcggcggata caagccgcag aacgggtgat gaaggaacaa attaaagagc tggagaacag
     9301 aattggttta tttgctgtcg gttatctaat tgatgagcta aaggaaaaat accaagagca
     9361 ggcagaaatc attaattatc tgcaggcggt gcagaaagat atcatggaaa atcttgatga
     9421 tttccgtcaa gctgaagaag aaagcacacc ctttccctgg ctgcggcgca aagaagcagc
     9481 gggagataaa taccgtgtca atttagtagt ggataacaaa gaaaccattg gcgcgccagt
     9541 tgtcgtagac acaaacccta cctactataa tttaattgga cgtgtggaat acgagaataa
     9601 attgggtgcc atgaccactg actatgctct gattaaggcc ggctccttac taaaagcaaa
     9661 tggcggttac ttaatactgc aagcccgtga cgtcttaact aacgctggtg cttgggaagg
     9721 cttaaagcgt gtactaaaaa cacaagaagc ttgcttagaa aatttaggtg agcaatttag
     9781 tctgctggca atgtcctcct tgcgaccaca agccatcccc gtacaactaa aggtaatttt
     9841 agttggtcct gccgagcttt atcacttgct ttatcattat gatgatgact tccgcaaact
     9901 tttcaaaatt aaagcagact ttgatacaga aatggatgcc agtcgggaca aaatgacaca
     9961 aatggctagt tttgtggcta gccacacaca gcgggcaggt ctgcgccact ttgatcgcac
    10021 aggagtagcc cggttggtgg agtatagttc acgccaagct gaacatcaac aaaaattaag
    10081 cacccgcttt aatgaaatta tggaaattat ctctgaagca gatgcttggg ctagtatcaa
    10141 gggtgatgat ttagtaaggg ctgaacatgt caaacaggcc attgaagaaa aaatatatcg
    10201 ttccaataca tatgaacaaa aattacagaa gttattagca gaagggacga ttctgctgga
    10261 tttaaaagga gaaaaagtag gtcaagtcaa cggcctctcc gtcttagata gtggtgatta
    10321 tgcttttggg cgagcgacaa gaattactgc tgttacctat ttaggccgcg aaggcattat
    10381 caacatagag cgagagacca aattgagcgg tcgtattcat gataaggggc tgctgacgct
    10441 gtccggttat ttagctgcca aatttgctca gaaggcgcca ctttctctct cagccagtat
    10501 tgcttttgaa caaatgtatg gtggaattga tggtgatagt gcctcaagta cagaactgta
    10561 tgctttactt tccagtctgg ctgaattgcc cttgcgtcaa gatattgcgg tcacaggttc
    10621 ggttaaccaa catggtgaaa tacagcccat cggtggggta actgaaaaag ttgaaggctt
    10681 ctttaaagca tgtaagctgc tggggttaac aggaaagcaa ggcgtgatga ttcctgtaca
    10741 aaacattatc aaccttaatt taaatgaaga agtaatcaca gcgatggggg aagggaagtt
    10801 tcatctctat gctgtgtcaa cgatcgcaga gggcattgag gttttaactg gagtagctgc
    10861 tggtcatctg gataaagaag gcgattaccc gacggaaaca gtttacggtc gcgttgccgc
    10921 caaactggca gactataata gcttgctgca gcaagcaaag tccgacaatg atgaggagga
    10981 gtaggatctc catcaccagc tttaattaat ttgaatttgg cagcattatt cgctacaatg
    11041 atgagaggat taagggaagg actgtcagat gtgaaaaggc aattaacagt agagcagctg
    11101 ctagctgaac gagaacattt ctatttagcc gacgtacgtt cacctaaaga atatgcagaa
    11161 gcccatatcc caggggctgc caatattcct ttgttagaaa atgaagagcg tgcgctagtt
    11221 ggtattactt ataaagatga agggaccgac cgagccaagc aggtcggtct ttcgctggtt
    11281 gccccccgtc tgccgcaaat gatagaaaag attcgtcggc aggccaaggg caaaaaagtg
    11341 gtgatctatt gttggcgcgg cggtacacgc agccgcagta tctgtgccat tatggaagca
    11401 cttaattacc ctgtatggca gctgcagggc ggctacaagg cttttcgccg ctatattcac
    11461 agctatttgt cacaagcgac agtgacgatg ccagtttttg tccttaatgg tttaactggc
    11521 gttggcaaaa cgcacattat taaaatattg cagcagcaag gttatcctgc tctggattta
    11581 gaagggatgg ccaaccatcg tggttcagcc tttggtgcag taggcctggg acaagctcgt
    11641 agtcaaaaag attttgaagc actactggcc ctatttctgc ttgagcatca tgctgcatcc
    11701 tatctggtga tcgaaggtga agggaaaaga atcggttcag ctacactgcc ggattttctt
    11761 ttccaggcga tggaagaggg acaacatctg cttttggaag cagatgtggc cgtgcgggta
    11821 ggccgcattg tggaagaata tcagggtcgt gctgaccact tgggggattt ggctgaggct
    11881 gtcaagacct taagccgcaa gttagggaaa gctaaaagtg aagaacttgc cagcttaatt
    11941 cgccaaggag aactggagac ggtggtaaaa actctttgta ccgattacta cgaccattat
    12001 tacagtgatt cacgtcaagg cagagaaaaa tatctggcag tggttagcgt taatgaatta
    12061 aacaaaggag ctgcagagat tttaacgatc atcaatgact atctggcagc gcagcctggt
    12121 tttgtgggga gagaggagta aacaaaaatg catattgttt tagttgaacc tgaaatacca
    12181 cccaacaccg ggaatatttc tcgtacttgt gcggtgacta atactacact gcatttagtc
    12241 aagcctttag gcttttccct ggacgaacgc catttaaaac gcgccggtct tgattactgg
    12301 cagcatctgc agctaatgat gcacgacaat ctagctagct tcctgccaac aatttctgca
    12361 cagtcctgct ggtttttaac tactaaaggc cagcgtttgt atactgatgt tgtctaccag
    12421 ccgtcagact atttattttt tggcaaagaa acagctggtt tacccgcctg gttattagcg
    12481 aaatatcccg aacgttgttt acgtgttccc atggggccgc agctgcgttc tttgaatttg
    12541 tccaacacag tggccatcgt tttgtatgag gcattacgcc aacaagattt tcctgcctta
    12601 atttagcata atacttgact tttaacggaa aagtagtatg ctaaccatat aaccccccgg
    12661 tgggggtggg gagggatgat attattgcga gggcccaaaa aggatgctac gctgcgtcgt
    12721 ttaaaaacag caggtggaca tttaaaggca gttgaacgga tggtagaaga cgataaatac
    12781 tgtgctgatg tgcttctaca gctgcaggct gttatcgcct ctttagagaa agtaagcaac
    12841 attattttgg aaaatcattt agagacttgt ttacgagctg ccatgcgccg cggcgaagaa
    12901 gaagaagtaa ttgacgaact gaaaacattt atgaaataca aaataaaata actcttacta
    12961 ggtgcgggcg cacaaagttc ctacaccttt agtacgtttg tttacgaaaa agatggagag
    13021 attatgacta aggccgtttt aagaacacag ctaataccgt gagcagagga ggaatagaaa
    13081 atgtctgaaa gtttgactct atcaattagc ggcatgactt gtaaccattg taagttgagg
    13141 gtagaaaaag ctttaaaaac cctaactggt gtggaaagtg tggccgttga tgtacaggct
    13201 ggtcgggctg aagtgcagta tgacccggca caagtaagcg gtgaatcact taagacggtg
    13261 gttacggaag cgggctacga agtcaaataa ttacgggcta tcaacagcag aaacctgtcc
    13321 tcttgggaca ggttttttta acggatatat atttttttca gacgtatatt gaatgaggag
    13381 gtgatattat gtctgccgtt aaattatcaa ttattagctg cttgctgatg ttaggcatgc
    13441 taatgctgcc atccttaacg ggagcgggta tggggagtat ggtcacccgg ctgtggcttg
    13501 cttttggtat tttggtgttt acaggcaatt atcttgctta ccaacaagag gctgcacagc
    13561 aagcagccaa ggacaatcag cgtactaggc agggagaaca ccctcttcgt caagaagtac
    13621 gtagtttaag ctaaaggtcg tttttgtccg ggcaggaaag ccatcttttc agctgatttt
    13681 ttcacctttt aagtgtataa tgagaagata gccaagctgt gtacttccct agtaatcagc
    13741 tggatggcta aaagattaaa atagacctgg tcaaagtaaa agggaatata ctgctccagc
    13801 aagaatcatt atagagaatc ggctacgagg tgaaaacatg aaaagtaaat catttgttca
    13861 tttacataca catactgaat attcgctgtt ggatggagct tgcaaaataa aagaggtagt
    13921 agcagaagca gttaagttag gtatgccggc tttagccatt actgatcatg gtgtaatgta
    13981 cggcgtaatt gatttttata aagcagcaaa aaaggcaggc ataaaaccaa ttatcggctg
    14041 cgaaatctat gttgcaccgc gcatgcggta tgatcgtgtt gctcaccgtg atagtaaaca
    14101 acaccatcta gtgctgctgg cgcgaaataa cgaagggtac caaaatttaa tgcagttggt
    14161 aactcgtggt tttagtgagg gtttctatta taagccccgc gtagaccatc agcttttagc
    14221 acgctataat tcaggtttaa ttgctctttc agcctgtttg gccggggaaa ttcccatggc
    14281 tctactagag aatcgccatg aagatgcccg tcaattagta caattctatc aagaaacctt
    14341 tggtagtgaa aacttctatt tggagctgca ggatcatggt cttgccgaac aaaaggccct
    14401 aaatccccag ctcattcagt tggcacggga cactaatacc cccttggttg tgacgaacga
    14461 tctgcattac atccgcaagg aagatgctgc accacatgat gttttacttt gtatccaaac
    14521 aggtaagacg cttgaggaca caaaccgtat gcgtttttcc acagatgaat tctatttaaa
    14581 atctcctcaa gccatggctg cccttttccc tgagctgccc gaggtggtga ctaattcctt
    14641 agaaattgct gaccgttgta atgtagactt tgaatttggc aacacccatt tgccagtgta
    14701 tgagatacca gcagactatc aagatgattt tagttatcta aaagaattgt gtactgctgg
    14761 tgcaaaaaaa cgttatggtg accccttaac agcagaagta gaagaacgtt taaactatga
    14821 attacaagta attcatcaga tgggctatga ttcttacttt ttaattgtgt gggattttgt
    14881 taattatgcg catcaacatg agatctttgt gggccccgga cgtggttcag cggctggcag
    14941 cttgatcgcg tatagtttag gtatcaccaa cattgatccg ctgaaataca atttgctttt
    15001 tgagcgtttt cttaaccctg aacgggtaac gatgcctgat attgatattg atttttgcta
    15061 tgaaaaacgt gaacgtgtga ttgagtatgt gaccaacaaa tatggcgccg attccgtatc
    15121 gcagattatt acttttggga ctatggccgc ccgtgctgcg gtacgagatg taggcagagt
    15181 tctgaatatc ccttatgctg aagtggataa gatttctaaa ttaattcctg ctgaactcaa
    15241 catcaccatc agtgatgctt tagataaagc acctgatttg aaagaactct atactactga
    15301 tggtagaatc catcagttat tggatttaag tatggcggtg gaaggtttgc ctcgccacgc
    15361 ttccatgcac gctgcgggtg tagttatttc cggcaaacct ttagttgaat atgtaccgct
    15421 ggccaaatca ggcgattcca tggttgctca attttccatg accacattgg aagaactagg
    15481 cttattaaaa atggactttt taggcttacg cacacttacg attatgggtg aagcagtgcg
    15541 tttgattaag caatctaccg gtcgggaaat agatttaaat aaactgccgc ttgatgatga
    15601 aaaaacatat acgatgctcg cacaagggga tacctcaggc gtctttcagt tagaatcaag
    15661 cggtatgcgt agtgtgctgc gggaacttaa acctactgtt tttgaagata ttattgctgt
    15721 ggtagcttta tatcgtcccg gccccatgga tcaaattcct acctttattc aaagtaaaca
    15781 tggtgatatt cccataaatt atttgcatcc tgtgcttgag ccaattttag aagaaaccta
    15841 tggcgtgatg gtctatcagg aacaaattat gcaggtggct tcggcggtgg ccggtttttc
    15901 gcttggcgaa gctgatttat tacgacgcgc cattagtaaa aagaagttag aagttttaaa
    15961 tcaacagcgg caattgtttg tcaagggctg cgtctccaaa ggtcatagta aggaactggc
    16021 taacgaacta tacgatttaa ttgtcaagtt tgcttcttat ggctttaata aatcgcacgc
    16081 tgctgcttat gctttggttg cttatcagac agcctactta aaggttaatt atcctactca
    16141 atatatggca gcacagttaa cgggtgtgat gtccactaca gataaagtgg ctggttacat
    16201 ttttgattgt aaaaagatgg gtatttcagt gctgcctccc tgtgtcaatt ccagcggcaa
    16261 gaactttact gttactgccg aacgtgaaat acgctatggc ttggcggccg ttaaaaatgt
    16321 tggcctgggt gccatagaat ccatagaagc agcacgagcg gaaaaaggag cctttacctc
    16381 gctgcgagat ttttgtgcgc gcatagattt acgtagttgt aataaaaaag tcatggaaaa
    16441 cttaatcaaa agtggtgcct ttgattggct gcaggccaat cgtaatcaaa tctctgcggt
    16501 cattgatgag acagtgatgg ccgcccagac attgagtcgt gagcagcaaa acggccaaat
    16561 aagtatgttt gatctagttg aggacgaaga tgaggcggag tggggacaac tgcatgatga
    16621 ctttccggcg ctgccagaac cgacaccgat ggagcggctg acaatggaaa aagaagcctt
    16681 gggattatat attagtggac atcctttgga agagtatact gaggttttga agttgagatc
    16741 tgaaattgtt cctctccatg agttagcaga tctgccggac gagcagtctg tcgtcacagt
    16801 tggtatgatc agcggcttta agcctattat tactaaaaaa ggcaaagcga tgtcattttt
    16861 aacgctggaa gatttaactt ccgaaacgga ggtagtagtt ttccccgaag tacatgaaaa
    16921 agcaaaacag ctgctgcaga atgaccaagt ggtgcttgtt cagggcaagg ttagccaccg
    16981 cgaggaagag gaggctaaaa tattagcgtc agccattgct ttactgcccc aagtagcaga
    17041 agaggtagtt attcgttgtc gccctgatgg cagtctcagt cagttacttt cgttaaaaga
    17101 cctgctgcag gggcagcaag gtatcttacc ggtctatctt ttatttccgg tggtacagaa
    17161 aaaggtttta cttagctcag actattggct gcctgaaaat gcaccgcaga ttgtggaaat
    17221 tcaaaacctg tttggtgaag atgctgtgac tgtgcagaaa gtcggataag ctactttctg
    17281 ccagggcaaa ttagtgatag ataaaaatgc aagtaaggag taatacgatg cgtaaaattg
    17341 gtgtacttac ttccggtggt gatgcgccag ggatgaatgc tgcgattaga gctgttgtgc
    17401 gaaagtcaat ctatcatggc ttggaggcat ttggctttcg acgcggttat tatggtttaa
    17461 tgaatggtga atatgaaatc ttacagctcg gttcagttgc tgatattatt catcggggcg
    17521 gtacaatgct gctgacggcc agagcaccgg agtttttaga aactggcggc cgcaaaaaag
    17581 caatcagcgt gattcatgaa agcggcattg atgctttgat tgtgatcggt ggcgatggtt
    17641 ctttgcgcgg cgcggcggaa ctggaaaaac tcggtgtgag cactgttggt attcctggca
    17701 cgatcgataa tgatatcgcc tgtactgact atacgattgg ttttgataca gcagtgagta
    17761 ccgtcaccga ggctattaat aaagtgcgtg acacagcaac ttcgcatgaa cgcatttatg
    17821 tcatcgaagt aatgggacgt tctgcaggct atattgcctt ggctgccggt ctttcaggcg
    17881 gtgcagaatc tatcctggta cctgaaattc cgtttgcttt aaatgaagta gccgagcgct
    17941 tacagcgggg tgtcaaacgg ggaaaattgc atagtatcat tctcgtggca gaaggtgctt
    18001 ccagcgccat agacattgcc gagcagctga aagcgctgac tagcatggat attcgtgtca
    18061 ctattcttgg ccatgtgcag cgtggcggca caccgacgtc ttttgatcgt atactggcga
    18121 gtcgtctggg tgcagccgct gtagatctgt tacgttctgg ctgtcatggg ctgatggttg
    18181 gcctgaaaga cggagaaatt aaaagtacac cttatgaaga agctttaaac caacaaaaac
    18241 agtttccaca cgatctttat aatttagcaa cgatcttagc tatctaaaga ttggcggcaa
    18301 ggaggaatta gatgcgcagg acaaaaattg tctgcaccct cgggccatct agtaatcagg
    18361 tggcagtttt aacaagctta atacaggcag gcatgaatgt ggcccgcctt aatttttcgc
    18421 atggtaatca tgccgaacat gctgccacgg tgtctgccgt tcgtcaggcc gcaaaagaag
    18481 tggggaataa tctggcgata cttttggata ctaagggacc agagatacga ataggtgctt
    18541 ttattgctga taaaattacg ctgcaggcag gggagtcgtt caaacttacg accaatcagg
    18601 ttgacggtac gcaagatcaa gtttatgtta attatccagg gattatcaat gatgtccagc
    18661 cgggcatgca agttctgctt gatgatggac taattgtttt ggagattatg gacatcacca
    18721 gcactgagct taactgccgc gtcattacag gcggtgagtt agcaaatcat aaaggggtta
    18781 atgtgcctgc tgcgcgttta caaatgcccg ctctttcggc aagtgaccgc gaagatatta
    18841 ttttcgcgat caagtttgat ctagacttta ttgcggcatc ctttacacgc aaggcagacg
    18901 atattcttgc cattcgggct attttggaag aattccgctc agacataccc attattgcca
    18961 aaatagagag tgaagaaggc gtggccgaaa ttgacaaaat cctctctgtt gctgacggcg
    19021 tgatggtggc acgcggtgac ttaggggtag aggtgccggc tgaagatgtg ccgcttattc
    19081 aaaaaagact gatcaaaaag tgtaatgctg ccggcaaacc ggtgattatt gccacgcaga
    19141 tgctagactc gatgattcgt aatcctcgtc ctacgcgagc agaggccagc gatgtagcta
    19201 atgccatttt tgatggtgca gatgcagtga tgctttctgg tgaaacagca gtgggaaaat
    19261 atcctgtgga agctgtgcaa acgatggcgc gtattgcggc cagagctgaa accgctttag
    19321 agtatgagtc taatttggaa actatctttg ctcctccaga acagagtgta acagatgcca
    19381 tcgcttatgc cacctgtcat gcagcgcagg aactaggagc aaaagcaata attacatcaa
    19441 ctcaatcggg ctttactgcc cgcaatgtag caaaatacaa gcctaaagca cgtattgtgg
    19501 cagttacgcc acgcaaagca gtggcgcgcc gcctgaattt aacttggggt gtcttcccct
    19561 tgttgtgccg tcctgcaacc aatacagatg aaatgtttgc cacagcgatt gaggctgctt
    19621 taggcagtga atatattaaa aatggcgatt tggtagtgat tacagctggt gtgccagttg
    19681 gcgttagcgg caccactaac ctcctgcgca tacatacagt gggagaaatc atcctgcgtg
    19741 ggacaggcct aggcaaaaaa gctgttaccg gaccagtaaa agttgctctg acagctgctg
    19801 aggcagctaa cctagaagaa ggccaaattc tcctcgcacg tgctactgat aaagcttatg
    19861 tcccttattt aaataaggcg gccggtcttt tggtagaaga gggcggcttg acatcacatg
    19921 cggctgtttt ggcactacat ctgggtctgc ccgtaattgt tggtgctgca gatgccgccg
    19981 ccatcgtcaa agatggcgag attgttactt tagatacagc acgaggtctt gtctaccgcg
    20041 gcaaggcaac tactttttag aagtgagttg atggcgatga aaaacgggga ggcgctaatc
    20101 cgcgtccggt acgaagaaac ggaccagatg ggtatggtat attacgccaa ttattttatt
    20161 tggatggaaa tagggcgtaa cgaatatttc cgtttactag gtatgtctta tagcgaagta
    20221 gaaaaaaatg gcatcttgct gccggtaact aaggccttct gccaatatca atcacctgca
    20281 cattatgatg atttgctgcg ccttgtcaca agcgtagcat ctttacagga tgtgcgcatt
    20341 gtcttcaaat acgagatctt taatgctagt acagacaact tattggctat tggagaaaca
    20401 gaacatgctt ttgttaaccc acaggggcgt ccagttgtgc tgaaaaaaca aaaccctttc
    20461 ttctggaaaa gaatgttgga agcaacggga gaaaatatgg agtagacaaa atgctaggaa
    20521 gactactact attaatgata atcgtaccga tcttggaaat ctttttgctg attgaagtag
    20581 gacaacaaat tggcgtcctg ccaacagtaa cgatagtgtt actaacggga ttgctcggca
    20641 tcatgctggc gcgtacgcag ggcttttatg tgcttacccg tattgttaat agtctgcgca
    20701 agggtgagct gccgggtgat gatatcttaa acggtctgct agtgctgatt ggcgcttcat
    20761 ttttattgac accaggttta atcacagaca ccgctggctt catcctgctc attcctcgca
    20821 cgcgtcgtac cgtaaaagta tggctgtatt acaagttaac aaaagcgctg aaagatggta
    20881 ctttacgcat cttccgccgc taaaaatgag caaagcacgc ctgcgggcgt gctttttaaa
    20941 ataagacaat tcttgcatag ctttgtttgc tactctacgg aaaagtcatt gataaccaaa
    21001 cctgtaggca gcttaggata gaaatatgtt gtcttctgtg gcattttatc accagctgtg
    21061 gcaacagccg tcacttcctg cactggcgtg ggattaagca aaaatgcagc ttgataggtg
    21121 ccatgatcga ctaaatttag tgcctctgtt gcatcacggg tgtattgcag ttgagttcct
    21181 tgagcacgtg cagcactatc gattcccaat agctcttcta gaattaaagt atgcagcacc
    21241 gttacatcta aagagcgcca agcagctgaa taatcaccat agcgttttac cagcgtggca
    21301 ttattactat cgcgcttaag agtcagctga tacagctttt ccccgccaag gtataataaa
    21361 atggcattgt atgtaggcaa gagagcagct aatttagtta actctgctgc taggagcgac
    21421 tgacgttgcg tgcgggggaa gtcaatagtc ttgacaataa aagatctgct taattgtgtg
    21481 agcaaggtat cttgtacgag ctgtggcaaa ccctttacta ggcggtgcgt ggggaagatt
    21541 actagaccgg gatcatgcag atttactaaa gccatcaggc aaaaaccaaa acgctcatcg
    21601 ccctgagccc tcatttcgcg ctggaagtgc aaggcagttt catagcggtg atgaccgtca
    21661 gcgatataaa ttgactggtt agaaaaaaac tcttgcattt ttgttagtgc ctgctggtca
    21721 ttaataaccc agagacgatg aacttcgtcg ttttcaccgc tgaaagtcac tagtggttta
    21781 ttttttttat aagtagcagc aatattttca actgtaaacg cggggtcatc ataaacgcta
    21841 aaaacggggc taaagttcgc ccggcaatga cgtaatagct gcagacgatc ggctttggct
    21901 tgaggcaagg tctcttcgtg gggaagaata attccttttt catattcctc aagtttaacg
    21961 ccggagatta aaccagtgcg tgtcagagat ttgcccttgg tgttaaattc ttgttcataa
    22021 agaaagactg ctggtttttc ttcatgctgc aaaatatttt gttgcagcca ctgtttaaaa
    22081 aagccggctg cccgggtata acggttgtcc tcagcactat cattgattct agtttcacca
    22141 tattctaaac gaatcacatt gtaagggttt ttttcataat aacgctgttg ttcttgttcg
    22201 ttaattacat catagggagg cgtgactaaa tcagccattt gtcctacttg agctgtgtta
    22261 tatcttaccc cacaaattgg tacgattgtt gccattttgc cttcctcctc cgtcaaactg
    22321 tcttgatttt accgcaagta aaaatttatt gcaaatcaag caaataggct ctattcctca
    22381 cttgcttgat ctttttgttc ttgatgaatt tcccaatgta agattagatt taagataaaa
    22441 cgcagagcaa caactgaagc taaaattact acttcggaca tactgcgcac gatcacagtg
    22501 cgcagtattt ctccggctaa cttaaaatcc agaccaaaga ccaaatagcg ggcaaagtta
    22561 agtcttgctg accggtcaca gcgaccggtg gtcagaaaat aaaaaaatgt ttttacagcg
    22621 gcagagagaa tgatgatggc gccaaagatt tcaagcaagt gagagcctaa agttaatatt
    22681 gtagtgagga atgattctaa agcatgaaga ttcacaaaga cggcctccct ttagagtgtt
    22741 taattactag tatagcacgt ttattgatcc tgtttattgt tcccgcaaac acagcatcta
    22801 caactcgatg aagagttgat tagttggttc ctgggaaagc tatatgttta atggaacctt
    22861 gtagtgcaac tgtgtgcgcc cgcaaatcag cttaagctta aatgtagaga ggatttatca
    22921 aatacttgat gacggcgcaa agctacgttg tttttttata gtattttaca aaatctttgc
    22981 agatagacaa taaaaaacaa taccctttgc atatactaca agaaaagcgt gctttattta
    23041 gtttcattgc agcgctggcg cttgtttaaa agtattgcta aaaaacaggg gggttgtttt
    23101 cttgcgcttg caaggtcagg cggttactat agctcggggt gacgtcgcta aagcattagc
    23161 gcaacgtacg atttactcgg cgcggcgcgt ggttccagaa ttcaatgatg tggcctcatc
    23221 aacagcggtt aaccgctgtg cctacttact gcgtagtact tttggcgaac caagttatgt
    23281 tgcccatcgt cctctagatg gtccggttga agtatgggta gtttccttaa aaaacgggaa
    23341 tggaattata tcctttgaat tgtggcaaaa cagtgaaatg ccgcgttact atattttcac
    23401 cgataaacct accccgatcg tagccaaatt attgcgacgt ctcggccgtt atctacgggc
    23461 gccaaatgtt tatgttgtgc cgaataagta aaatgtccgc ttctagcctg tactttggct
    23521 gtatctaccc cctgggttca cttgctttgc ctgctggtcg gcaactgaaa aacaagatag
    23581 tttaaaaaaa atactcttat ctttagttca tacttgccct tcacaggatg aagatctcct
    23641 gataaaatga aattagaaat aaaaggaggt tttaacaatg ctcgtcaaag atagaatgtc
    23701 acctcatgtc tacacgataa caccagatac accgatacct gatgcattgc agctgatgga
    23761 ggagaagaaa atccgccgcc taccggtgat tgataaagac cgcctggttg gtattgtgac
    23821 attattggat ctggtgcgag cttcaccatc accagccacc agtttgagtg tctgggaact
    23881 taattacctc ctggcgaagt taccggtaaa agatgtgatg gcaaaacaag tatattgtgt
    23941 aacgcccgaa acgaccattg acaaagcagc atctttaatg cgtgaacata gtattggcgg
    24001 cctccccgta ctggatggag agcaaattgt aggtatgatt acagaaaccg atatttttac
    24061 tgcctttcta gagatgctgg gagtccatcg cgggggcctg cgcctcacct tggaacttac
    24121 agaacggcat ggcgccttag cagacattgc cttattattc cgagattttg gtgtaaatat
    24181 tgtcagtgca gttgctttta tcagtgacga ggatcaagag ctggcgcatt ctgtgtggcg
    24241 tctggaaggc tgcgacgatg tagaagggtt aatcaagtac ttacaggaaa aagaatataa
    24301 ggttattcac tatagtttaa atggtgcagg tacagaggta taatcgaaaa cggtaaataa
    24361 aagaagagtg cctaacatta atcaaatgta ggcactcttt tgcagtatct tatgaggttt
    24421 gcgctcgacg cattgctcga gttttattaa actgagcacg ttcttgtttt tttagcagtt
    24481 ttttgcgcaa gcggaaactt tgcggcgtaa tttctaccag ctcatcgtca gcaatgaact
    24541 caatggcttc ttccaaacta agattgcgcg gttctttaag acgaacagtt tcttcagaag
    24601 tagaggcgcg catgtttgtg agatgtttct tcttacaaat attaacctct aaatcaactt
    24661 cacggttatt ttgcccgaca atcatccctt catagacatg agccccgggg acgacaaaaa
    24721 gtgtgccgcg ttcttctgcg gcatgtaagc cgtaagccgt agcctcgcca tcttcccacg
    24781 cgatcagcgc gccttggaaa cgagaggaga tttcaccttt gtggggctcg taactagaga
    24841 aggtatgatt cataatccca ttaccccgtg tttcacttaa atattcggag cggaaaccta
    24901 ttagtccgcg tgccgggatg cgaaattcta aacgaatttg tcccggttcg gtgggcgtca
    24961 tgtctaccat ttctgctttg cgctggctga ggttttccat aattgcgccc atatattctt
    25021 ccgacatgtc tagcactaaa tgttcaattg gttcatggag tttaccatca atttttttga
    25081 aaatagcttc tggtttactg acctgtaatt cataaccttc acgtcgcata ttttcaatga
    25141 ggatggagag atgtaattcc ccacgccccg ccactaaaaa actatctgga ctatctgttt
    25201 cttccacttt taaggaaaca ttggtttcta gctcgtggaa gagtcgctcc cgcagcttac
    25261 gtgaagtgac aaattgacct tcacgaccgg caaagggact attatttaca acaaaataca
    25321 tggttagtgt aggctcatca acggtgagta ccggtaactg ctcaaggtga tccggatgac
    25381 agatggtttc accaatattg acattatcta aacctgaaag agcaataata tcaccagtca
    25441 tagcgttatc tgtttccatt ttttttagcc cggaaaaaac aaatacctta tttactctgg
    25501 cttgttctac ggaaccatct gcatggagca gagcgacagc ttcacctgcg gcaattgtgc
    25561 cgcgtaagat ccgcccaatg gcatagcgcc caacataatt atcatgggtt agattagcca
    25621 cgagcatttg aaaggggcca tgctcatcgc tggcaggggc aggtacatgg gaaacaatta
    25681 cctcaaaaag tggttctagt gaggagctgt tttcatcagg ttcgaggcta gccataccgg
    25741 tgcgagccga ggcataaatt acagggaaat caagctgctc gtcattggcg cctaactcca
    25801 caaagaggtc gaaaaccata tcgacaactt gtagtggtcg tgcatcgctg cgatcaatct
    25861 tattgacaac cacaatgggc agcaagccca actctagtgc tttacgtaaa acaaagcgcg
    25921 tttgtggcat gggaccttca aaggcatcta caattaaaag cacaccatca accatggata
    25981 aagctcgctc tacttcgcca ccaaaatctg cgtgtcccgg ggtgtccaca atattaatct
    26041 ttactcctcg gtactcgacc gcagtatttt tagccaagat ggtaatgccg cgttcgcgct
    26101 ctagatcatt tgaatccatc acacgttcta caacggcttc attactgcga aaaatgccgc
    26161 tctgccgtaa cataccatct acaagagtag ttttaccatg atccacatgg gcgataatag
    26221 cgatattgcg aatttgcatt tttttgcctc ctatttgcac atctttttta gtataacagc
    26281 attgtggcgc agtaacaaga aaaaatgtgt gataaacagt taactagaca gcagcaggga
    26341 aagttagctg ctttaaggaa taaagatatg tgtaacagca gatattggag gtttgccagt
    26401 gagtgttgca gcagttcatc atgtaaaaaa atattttggt gccgaactca ttctcgatga
    26461 tgcttctttg caattgcacc cgcgtgagcg agtcggttta gtcggcccca acggagcagg
    26521 taaaacaacg ctccttaata ttattgccgg agaagttatt gctgatgagg ggacaattac
    26581 gctggcacga ggtagttcac taggttatct gaagcaaggc tcaaaaagtt ctgctagcgg
    26641 aacaatggaa gtagaactgc gcagagcctt tgccgaaatt gatgctttag ctgaacagct
    26701 taattcctta gagcagcaaa tggctgcacg ttcctcacaa tctgataagc agctagaaga
    26761 aattatgagt acctatggcg aattacgcca ccgttttgaa gatgctggtg gttatttgat
    26821 ggatgcccgt ctccgtgctg ttgttaatgg tttgggcttt caaatgcagg atttaccgcg
    26881 tactatagct acattttctg gtggagaaca aacgcgtatc cgtttagctc gtttactgct
    26941 ggaagaacct gaggtattgc ttttggatga gccaactaat catttagata tagaagctgt
    27001 cgagtggtta gaaaaatatt taaatgactg gcacggtacg atattgattg tttcgcatga
    27061 ccgttatttt cttgatcgtg tggctactcg tattttagcc ttggagcatg gtcagcttaa
    27121 aagctaccag ggtaattatt cagcttatca gaggcaaaga gagcaggaaa gaattacaca
    27181 ggaaaaagcg tatgctaaac aacaggagta cattgaaaaa gaaacaacct atattcgcac
    27241 cctcggtaca ggagaaaggg aaaaacggca agcaaaaagt cggcaaaaaa gattagcaaa
    27301 actgcaaata atggacaagc cgcaacaggc tagagcgatg gcacttgatt tcggtttttc
    27361 cggccgtagc ggtgaaattg ccgtgcgcat ggaaggagtt gctaaatatt ttgctgacca
    27421 ggcagtgttt agtcaggtag attttgtgct ccgttggggt gagcgggtcg cactggtcgg
    27481 ccctaatggt tcaggtaaaa gtacattact taaactgatt gccggggaat tacagccgga
    27541 tgtaggtcaa gtttgggttg gaccaagtgt taaactggtc tattttgacc aacaccaaca
    27601 gattatcgcc agtgataaaa ctccacttga ggaaatcatg gatgctgcca gcatgacgat
    27661 tacagaggcc cgtagctatc ttggacgttt tctttttatc ggagatgatg tctttaagcg
    27721 taatgctgat ttaagcggtg gtgaaaaaag tcgcttagcc ttagccaaac taggtttgga
    27781 tgaaggcaac tttttggtct tggacgaacc aactaatcat ttagatatca caggagtaga
    27841 ggagttagag gaagcaattt gcagttttcc ggggacttta attgttgttt cccacgatcg
    27901 atatttcgtc agccgtacca cggaaaaggt acttgatgtg gcaaaagggt gcgttactta
    27961 ttatccggtg ccttacgatg aatatgtgca ggagcgggag aaaaaagctg tgattgaaat
    28021 tgatcctgcg gaaagcaaaa aaatacagcg acatgcagag cagcgacgcc aacgagaagc
    28081 agaattacaa caacgtcgtg aattgcgcaa gctgacagaa gaagtagagc agacagaaac
    28141 agaaattagc agtctggaag agaagcttgc tgttttagaa gcggagctgt tagagcccca
    28201 agtctattct gattatcagc tggcagcaga aaaagggcag gagttggcac aagttaaagc
    28261 tcgcttacag ttgctatatc ggcagtggga gttgcaaaca gaacaagtag aaaagttcaa
    28321 aggtaagata attttaggtt aaagattaat ccgacagcga gctgccggat taattatgtt
    28381 aggacttaga cagctcggta atcagtagta tgactgggct aaaatatcgt ttactgagta
    28441 ttactaaagt gtcatgctct ttatattggt gtaaattgtc gctatatgta aagtatgact
    28501 tgttttattt agtgtagatt agatagaata ggaagggata ttgtaaataa tctgatggag
    28561 ggattcgatg aattcaattg aagaacgtgc ggtaaatgca attcgttttt tagcggttga
    28621 cgcaatagaa aaagcaaatt cgggtcatcc cggtttgcct atgggtggtg cccctatggc
    28681 gtatattctg tggtctcgtt ttatgcgtca tagtcctaaa catccgagct gggagaatcg
    28741 tgatcgtttt gtgctttctg ctggtcatgg ttctatgctt ctgtatgccc tcttacattt
    28801 atttggttac ggtctttccg tggcagactt gcagcaattc cgccagtggg gcagcaaaac
    28861 gccaggacat ccggaatacg gccatactgt gggagtggaa acgaccaccg gacccttggg
    28921 acaaggattc gcaaatgctg tgggtatggc catagcagaa cgacgtctgg cagctgagtt
    28981 taacaaacct gaattcgagg tagtaaatca ttatacttat gtgtataccg gtgatggctg
    29041 tttaatggag ggtgtgagtg ccgaagctgc atctttggcc ggtcatttaa agttaggtcg
    29101 cttaatctgc ctttacgatg ataacgaaat tactattgat ggcagtacta aagttgcttt
    29161 tactgaggat gtggcgcagc gttttgccgc ctatggctgg catgtccaaa agattgctga
    29221 tggcacggag ctgtccgcta ttgctgaagc tattgccaag gcacaacaag aatcagctcg
    29281 cccctccttg attatggtgc gcacggcgat tggttatggt agtcccaata agcagggttt
    29341 atcagcggcc catggtgccc ccttgggcag tgatgaagtg aacctgacta aagaaaattt
    29401 aggttggccc ttagaacctg cattttttgt cccagatgag gtgcgtgaac attttgcggc
    29461 gctgactaca caacaggaag cagcgtatac cgcttggcac gatttgttcg ctgactatcg
    29521 tcagcgtttc cctgaagacg cgaaacgttg ggatgactgg catagtttgg atgtgccagc
    29581 tgctttagca gaggaggaag agttgtggca gtttaccaaa ccgctggcta ctagggctgc
    29641 ttccggccag gtaatgcaga taattgctaa acatctgcct aatttactgg gcggttcagc
    29701 ggatttaaat gcttccacta aaacatacct gaaagctttt tctgatttcc aggcagaaaa
    29761 ctactgtggt aataatctgt tttttggcgt acgcgaacat gctatgggtg ctattgttaa
    29821 cggcttggcg ctttatggcg gcttacgccc ttacagctca actttctttg tctttgtaga
    29881 ttatatgaaa cctacactgc ggttagctgc cctgatgggc ttgcctgttg tctatgtatt
    29941 tacgcatgat agcattggtg taggtgagga tggacctacc caccagccag ttgagcaact
    30001 ggcaaactta cgctctatcc ctaatctcca tgtgctgcgc cctgctgacg gacgtgagac
    30061 agcggctgct tggttgtcag cagttaagcg agtagacggg ccaaccgccc tggtgcttag
    30121 tcggcaaaaa ctgccgcaat tatctggtac aggtcttgaa gcgcaaaaag gagcctacat
    30181 cctcagcccc gagaagggac aagctccagc agcaatactg ctggcaagtg gttcagaagt
    30241 tcatttagcg ctaggggcac agcaagaact ttggaaaaaa ggcatagatg tacgtgtcgt
    30301 cagtatgatg agctgggaat tatttagggc acaatcaccg gtctaccgtg aagaagtctt
    30361 gcctaaggcc ataaaaaagc gtttagctat tgaagcggga catactatgg gctggcacag
    30421 ttttatcggt gacgaaggtg agatgatcgc cattgatcat tttggcgcct ctgctcccgg
    30481 tgagatatta atggagaagt ttggttttac ggtagccaat attattgaga aagtaacata
    30541 cttattgcaa aaataatagt aggggcgaac tgtgttcgcc cgcacaatta aaataactcc
    30601 cttcgctttt tgtctagcga agggagtttg gattatttca ttaaactgca gatgttattg
    30661 gtgaattcca caggatcttc gatgtttata ccttcaatga gtaaagcttg gttaaagagc
    30721 aaattggtat aaagtttgag tttatcttga tcctgctcgt acgcttcctg cagtgactta
    30781 aatacttcat ggtttgtatt aatttctaag accttatcag ctttgatgtt ctggttgtta
    30841 ggcatagcat tcaatatttt ctccatttca atcgatacct caccggcatt agctaaacag
    30901 acaggatgat tttttaatct cttggaagca cgtacggagg atacttgctc ggataaaaca
    30961 tcttgcataa aatcaaatag cttttgttca gctgctggct cagcagtagc cggtgtttca
    31021 tcttcaatgc ctaaatctgc attggaaata gatttaaact ctttatcttg gtaggcagct
    31081 aacattttta tggcaaattc atctatatct tcactgaagt agagaatttc aaatcctttt
    31141 tcctgcaaca attcagtctg gggcattttt tcaattcttg ccacggactc cccagctgca
    31201 tagtagatat attgttgctc agacttcatc cgacctacat attcagctaa agttaccagc
    31261 tttttctcgt gagaggaata gaagagcagt aagtcctgga ggtcttcttt gtggctgcca
    31321 aactcactat aaacaccaaa ctttaattgt cggccaaagg tctgaaaaaa cttttcataa
    31381 gtttctctgt catctcgtaa aagtttttct aactcat
//
LOCUS       DASRMS010000165         1375 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_715513, whole genome shotgun sequence.
ACCESSION   DASRMS010000165 DASRMS010000000
VERSION     DASRMS010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_715513"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..470)
                     /locus_tag="VFC74_07120"
     CDS             complement(3..470)
                     /locus_tag="VFC74_07120"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25143.1"
                     /translation="MNHAISLDTAALVTAVSKRTLWRRLDDGQITRQENDSRGRAMLT
                     LVDLVPMLCVSVDPEDYELLVDADAGNADAQNDLAQLFLDANRPDIALHWLHLAVDQE
                     HADAIHNLAMLYIKGIGLEKDKTKGLMFLAKAASLGHFIAKQQIATLHFREPS"
     gene            835..1356
                     /locus_tag="VFC74_07125"
     CDS             835..1356
                     /locus_tag="VFC74_07125"
                     /inference="COORDINATES: protein motif:HMM:NF012342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HZK25144.1"
                     /translation="MKAQMQKGFTLIELMIVVAIIGILAAVAIPAYQDYTVRAKVSEV
                     LVAGASAKSAISEAFQTAGMSGVKSAAASSSKGSAAEPVSKYLESITVDGDSGVITLT
                     TTNDDSLPTDARQETIIMAPYTSGGKLAAGAEGSIQWACSAGTKVQAEERSPGLIATA
                     GTLPAKYVPTECK"
BASE COUNT          392 a          301 c          318 g          364 t
ORIGIN      
        1 agttaggagg gttcacgaaa atgtaaggtg gcgatctgct gcttggcgat aaaatggccg
       61 agtgaagcgg ccttggccag aaacatcagc cctttggttt tgtctttttc taagccgata
      121 cctttgatat acagcatagc caggttatga atggcatcgg cgtgttcctg gtctaccgcc
      181 aagtgcaacc agtgaagcgc tatatctggt ctgttagcat ctaaaaacaa ctgcgctagg
      241 tcattttgag cgtcggcatt acccgcatca gcatcgacaa gtaactcgta atcctctgga
      301 tctactgaaa cgcataacat gggtacaaga tccacgaggg ttagcattgc gcgacctctg
      361 gagtcgttct cctgacgggt gatttgccca tcatccagcc tgcgccaaag cgtccttttg
      421 ctgacggcag tgacaagagc agcggtatct agacttatcg catgattcat tttacgccct
      481 tcaatttgag tttggcacag taatcgccgt gactttctgt ttttggcaca acttgtcaca
      541 tgccaacggc tgttttggca cactctagca tatgcttatg gcgatttggc gcacttaatg
      601 atgcgacaat gactattttg gcacagttaa agaggttttt ggcacacctt gtcaaacgcc
      661 acaggttgtt ttggcgcacc tgaaattatt cacgattcga gataaatact gataaatagc
      721 taagaacagg cgaaataaac ctatttcagc caatttctta ttaagaaaag ttggcatgga
      781 agctgcatta tacttattag cccaacggct atcttataaa ttggagcgtt aaaaatgaaa
      841 gcacaaatgc aaaagggttt taccctaatt gaattaatga tcgtagtagc gattattggt
      901 attttagcgg cggtagcgat tcccgcttac caagactata cagtacgtgc aaaagtatct
      961 gaagttctag ttgcaggtgc ctctgcaaaa tctgcaatta gtgaagcatt tcaaactgcc
     1021 ggcatgtctg gagttaaaag tgctgcagca tctagctcaa aaggcagtgc cgctgagcca
     1081 gtcagcaaat acttagaatc aatcacagtc gatggggata gcggagtaat tactctaacc
     1141 accactaacg acgatagcct gccaacagat gcaagacagg aaactattat tatggctcca
     1201 tatactagcg gtggtaaatt agcagctggc gcagaaggct caatccagtg ggcatgctca
     1261 gccggtacaa aagttcaagc agaagagcgt tctccgggtc taattgcgac agcgggaaca
     1321 ttacctgcaa agtacgtacc tacagagtgc aagtaattag ttactaactt aataa
//
LOCUS       DASRMS010000166        24387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_44165, whole genome shotgun sequence.
ACCESSION   DASRMS010000166 DASRMS010000000
VERSION     DASRMS010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 24387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24387
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44165"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1345)
                     /gene="radA"
                     /locus_tag="VFC74_07130"
     CDS             complement(<1..1345)
                     /gene="radA"
                     /locus_tag="VFC74_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004077661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HZK25145.1"
                     /translation="MARSKTVFYCSQCGYESPKWLGKCPGCGVWNQMVEEVRQPAKAS
                     VGRSSAAKVVPLSAIGDTEQAGRLTTASRELDRVLGGGLIPGSLVLIGGDPGIGKSTL
                     VLQAAQGLGSAGKKVLYIAGEESPAQLLLRAKRLGVDSEQIMVLPETDMSILEEQVRG
                     LRPDVVIVDSVQTVYSPHLSSAAGSVSQLKEVTTTLLRLAKRENIAVLLIGHVTKEGQ
                     IAGPRVLEHMVDCVLYFEGDRQHLYRILRAVKNRFGSTHEIGLFTMDAAGLREVENPS
                     EWLLSQRLLDAAGSVVTASMEGTRPLLVEIQALVTPTRYGNPRRTATGVELNRVALIL
                     AVLERHVGVHIQDFDVFVNAAGGVRLVEPAIDLAVAASLVSSFRDQAVPRELLFLGEI
                     GLAGEVRGIPHLEQRLHEGARLGFKRAIVPKFSLAGLQNSAGLQISAVATVAEAIN"
     gene            complement(1596..2171)
                     /gene="pyrE"
                     /locus_tag="VFC74_07135"
     CDS             complement(1596..2171)
                     /gene="pyrE"
                     /locus_tag="VFC74_07135"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009064249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HZK25146.1"
                     /translation="MISKERVLDVLQKTGVLLEGHFLLTSGRHSDRYLQCARVFQYPD
                     YAEEFCQALAAMFKEEQVDVCIGPALGGVVIAYETARACKTRGIFAERDQAGLMTLRR
                     GFSIEAGERVLVLEDVVTTGGSVREVIALIRELGGNVVGVASIVDRSNGKVDFGVPYQ
                     ALIRLDVASYDAEDCPLCKQGTPAVKPGSRK"
     gene            complement(2204..2911)
                     /gene="pyrF"
                     /locus_tag="VFC74_07140"
     CDS             complement(2204..2911)
                     /gene="pyrF"
                     /locus_tag="VFC74_07140"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HZK25147.1"
                     /translation="MHSKIIVALDVDAEEKALALTEALHDYVGAFKVGLQLYNSVGPS
                     IVKKIRSAGGKVFLDLKFHDIPNTTARAAEAVVGLGAFMFNVHAGGGKKMMLAAAQAA
                     KKKAAALGVPAPLLIAVTVLTSLSETELQEELGVQRALPEQVAALASLTKEAGMDGVV
                     ASAREIPWIRQACGDDFLIITPGIRPAWAASNDQERIVTPREALAQGADYLVIGRPLT
                     AAPDPQQAAKRLLAELI"
     gene            complement(2934..3857)
                     /locus_tag="VFC74_07145"
     CDS             complement(2934..3857)
                     /locus_tag="VFC74_07145"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006520705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HZK25148.1"
                     /translation="MTPDMTVKLGQLTLRNPVMPASGCFGYGEEYAPFYDLAKLGAIV
                     VKGTTVEPRLGNAPVRIVETSAGLLNAIGLQNPGVAAAKEKIRQLAPYKTPVIVNVAG
                     HSSNDYQHVIEALEEEPTVAAYEVNISCPNVQAGGLAFGTDPAVVAALIKRLRRVTEK
                     TLIVKLSPNVTDIVAIAQAAESAGADALSLINTLVGMAIDAEKRRPVLANLTGGLSGP
                     AVKPVALRMVWQVTEAVRIPVIGMGGISCTADAIEFLLAGATAVAIGAANFINPTVCP
                     EVAAGIAEYLKRHHFQSVQEIVGLAKLTSQE"
     gene            complement(3854..4612)
                     /locus_tag="VFC74_07150"
     CDS             complement(3854..4612)
                     /locus_tag="VFC74_07150"
                     /inference="COORDINATES: protein motif:HMM:NF012402.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HZK25149.1"
                     /translation="MPEKIEMEIIKHQAVSPEVRRLTLAGEITAQPGQFVQVGVTTSY
                     DPFLKRPLSVHDWRAGELTLLYRLAGRGTCLLSAKKVGESVTVVGPLGQGFPLGGQRP
                     AIVVAGGIGAAPLFFLLRSLQAAGKEVTFFYGARSAAELVLREQFQALATSYVEATDD
                     GSLGRKGLVTDLLQAALLTDKADIYACGPEPMLRAVAKLAAGYQCRCYVSLEAQMACG
                     VGACLGCVIPSTNGTYVRVCVDGPVFAAEEVFAE"
     gene            complement(4628..5920)
                     /locus_tag="VFC74_07155"
     CDS             complement(4628..5920)
                     /locus_tag="VFC74_07155"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HZK25150.1"
                     /translation="METVVRGGVVVDPSQLYHGNCDILIREGKIVELAEGIATRGRLV
                     IECEGRTVIPGLIDVHTHLREPGGQEKETIRTGSRAAAAGGYTSITALPNTQPVCDNQ
                     QKVQQLWQRIKEDSLVRIWPLGAITKHSAGQELTDIAQMVQAGIRAVTDDGRGVQQAE
                     LARRALQECAKLKIPYCEHCEEESIAGSGQIHPGKVAKQLQLPGIPVSSETLMMVRDA
                     MLAKECKAHLHIMHVSCAETVDWLRLLKTAGVVVTAEVTPHHLLLSEEAVLKYGTLAK
                     MKPPLRTEADRQALITALVDGTIDLIATDHAPHTTSDKERGFSLAPFGIIGLETAFPL
                     LYTHLVRTELLTLSGLVERMSCRPAALLDIPHGTLKPGFAADLTIIDLEQQQVIDKEL
                     FYSQSRNTPFHGWLVYGLPVLTMVEGKVVMHHGEIKIN"
     gene            complement(6130..6573)
                     /locus_tag="VFC74_07160"
     CDS             complement(6130..6573)
                     /locus_tag="VFC74_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25151.1"
                     /translation="MKVYSFTYTLHHILLLKLIANFPFDRSRTLHNFLFLAAANTPAA
                     ERAGIRYDFYRGTVSVYSFDIQGLLTDLKKGDLLEKDTLTLTKEGLDFYYQVAPLLRY
                     ERFPENCMKMALQYQNSLWRINHEIIFHPLFRKYKLGRKIILPAV"
     gene            complement(6596..7120)
                     /locus_tag="VFC74_07165"
     CDS             complement(6596..7120)
                     /locus_tag="VFC74_07165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25152.1"
                     /translation="MQIRDMQRMVAFLPIDKTGIGQCVQLYFRDGETSVAAVGLRSFL
                     QQLAKLYLLNLSEMRKRLGPIIGCKNLVPLALSPIIIFVPLKTRVPSVKGDCAYGYFK
                     LRSLLEVLAKPAPSTLILKGGHRLTIKQSYRLVCRRLRLARKLENYLFTQYYEAMEGE
                     RHNIYFFSQNEKRV"
     gene            complement(7190..8362)
                     /locus_tag="VFC74_07170"
     CDS             complement(7190..8362)
                     /locus_tag="VFC74_07170"
                     /inference="COORDINATES: protein motif:HMM:NF024579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK25153.1"
                     /translation="MKVGDENMKNPTKGTGNDINREKVVPFEREAGFYLRKGMLYYQK
                     NKHDKALQYFKKAVSVEPQNPFNHYNLACMLSKQGQLKEANRVFLHIINRLDETMTDC
                     YFLLAVNYGLLDEMEKSREYLLRYLQTDPEGELSLEAAELLDAFDDDDDLYTLPTYAQ
                     PDFVLEDIITTAKTEELTAHYLNNNRYRRALDSMLYARTDQYKEDILSIYGKLGGKVA
                     CRALRKFVRNPWVKERFRQMALLELKNLGESKVQIFSAGQLREVDLAKYPLRTPTWRP
                     EWQQVIDCTIGSMRASNCYDEGFLADVQAIWLDFINTVYPDTPRISKVQTWAAALEYS
                     LARFHFLDLTQKELAAEYGVSCASISNKFREINHALQLEERAYQNMLSYLQGEPEH"
     gene            complement(8359..9321)
                     /locus_tag="VFC74_07175"
     CDS             complement(8359..9321)
                     /locus_tag="VFC74_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK25154.1"
                     /translation="MEKQFISELKVGDAVQSDFAVTEKALNPFSQPNRAGEYYLRLQV
                     ADSTGTMRAVAWEQGPELAALFEVGDVVRVRGEVSNYRGSQLIIYSLEPVPLAEIKYE
                     HFQKVAPRSCEEMVGELQSLISSITNPYLHSLLQAFFADEAFLQRYSQAPAARTVHHN
                     YVGGLLEHSLEVAALCQHFVQLHSQLDLSLVLCGALLHDVGKLEEYEVKGMSIELTTA
                     GKLLGHIIMGKDMLEAKIKDIVDFPPDLHMELVHLILAHHGQKEWGSPEIPRTFAAYA
                     LHHADLISARLNQFAQVSRKGEHSNNWTEYDRLLARDIYLGKAE"
     gene            complement(9817..10734)
                     /locus_tag="VFC74_07180"
     CDS             complement(9817..10734)
                     /locus_tag="VFC74_07180"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HZK25155.1"
                     /translation="MTKSNLQKVLESGAFAVTGELGPPKSGEREVIEHHASHLKGCVD
                     SVNITDNQTAIVRTSSIAAGKIILDMGIEPNVQMTVRDRNRIAIQADVLGAAALGMKN
                     VLCIQGDHASLGSEPQAKNVNDLDSMNLISTLKKMRDEQEFIGGDKLDAPIDLYIGAV
                     ANPFADPFEYRPYRLAKKIAAGADFIQTQVIFDMERFELYMKKVVDMGLHEKVRIIAG
                     ITPLKSLGAARYMKKFVAGMIVPDHIIDRLKGAKDKKAEGVNMAIEMIEHLKTIEGVA
                     GVHIMAVAWEEIVPEIAERAGLLPRPTFD"
     gene            complement(10734..11402)
                     /locus_tag="VFC74_07185"
     CDS             complement(10734..11402)
                     /locus_tag="VFC74_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HZK25156.1"
                     /translation="MIVAERKPLKEILANLEPFKKVLVLGCGECVTVCMAGGDKEARE
                     TALALSIARNKAGNPIEVKTHTVERQCEPEYLESVRHLVEEADVVVSLACGVGIQNMN
                     MYFDDKRTLPGVNTTFMGMPTAQGEWMENCLGCGNCVLDETMGICPIARCSKSMLNGP
                     CGGSQNGKCEVNKDLDCAWHLIVERLMKFNKLDALQEIKEPKDWSTSHNGGPRKMIRE
                     DVKL"
     gene            complement(11403..13496)
                     /locus_tag="VFC74_07190"
     CDS             complement(11403..13496)
                     /locus_tag="VFC74_07190"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK25157.1"
                     /translation="MSNIKYDLSSAVPEYPPCQGGCPVHTDVQEYVRQISVGNYDKAV
                     EAITAPNPLASVCGIICAHPCETECRRKEVDEAVSIRALKRFALENGAWPEVKKAEVT
                     RSEKVAVVGGGPAGLAAAKDLAEMGFAVTVFERSSEAGGAITNFIPTYRLPMEAVRKD
                     VGRVAALGVEIKTGVELGKDVSLDELAKDYQAIILALGLPTSRSLNLPGADAKDVMLA
                     LPFLSSVKFDDFKFEPGRNVLVIGGGNVAMDVARSAVRVGAESVQLMCLECHDQMPAN
                     SWEIEEALEEGIVMHPGWGQQQILVDDNGKVTGVEVKEVLSVLDEQKRFNPQYGANEK
                     VIPCDVVVLAIGQANDDLSYLTTSGVELTERGVLKYNRHTLQTTRPDVFVAGEVAFGP
                     GLAVEALASGQKVAQAVNAYLDDVPFDTDFVDRFTTLEPLAASTVEKVKRIARQEVPN
                     LDKETRTQHFDMVEVGYDERAALCESRRCLDCAAGAECLDELCAVCLTCLRICPYGVP
                     TIDENGVFSIRNEECQACGLCVGICPAHAIRFRDQYAQHALDQLEDAVKLAQGNGGPA
                     TVVFCCSYGAFAERSFLNYVNNEKPQNLAIVRVPCVAKVEVEHILNAFNLGAEAVYVA
                     GCQEEDECTYQDVDKWATKRTGRAQEILTELGLEKERVEYATLSVDEVRDFKNVAEKY
                     LKKVDAVLNGEGGEE"
     gene            complement(13526..14320)
                     /locus_tag="VFC74_07195"
     CDS             complement(13526..14320)
                     /locus_tag="VFC74_07195"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HZK25158.1"
                     /translation="MFIIGERINGMFKDIAHAIREQDPRAVQDWARRQDEAGARYLDI
                     NTGPAASDPIKAMRWLVEVTQEASDLPLCLDSTNYDVIEAGLEVCKRPAMINSVPAEW
                     PKMERVFTMAAKYDAAVIGLAMNEEGIPKDADTRVALAMELVAAADSYEIPMENLLID
                     PLVLPCNVGQDHGPEVMRTLQQVKLLSDPAPRTVVGLSNISQGTKGGRSLINKTFAAM
                     AIANGLDAAVGDACDADLMAIIATARVIMNLDIYCDSYVDIYKNQK"
     gene            complement(14382..15362)
                     /gene="cdhD"
                     /locus_tag="VFC74_07200"
     CDS             complement(14382..15362)
                     /gene="cdhD"
                     /locus_tag="VFC74_07200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015944232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CO dehydrogenase/acetyl-CoA synthase subunit
                     delta"
                     /protein_id="HZK25159.1"
                     /translation="MAFSAMKEKYRNKVNTVVLGATQEEGGTRGYTVTVGGESALPFL
                     HFEGEIPNRPVVALEIWDIAPTDWNESFTPYYGDVFDNPTQWAKKCVEEYGADVLVVR
                     LKGADPDIADASPEDCVKVVQDVLAAVPCPLIVLGCGKAEKDNQVLPAVAEATAGENL
                     LIGSAEQENYKSIAAACMVHKHSLIANSPIDINICKQLNILITEMNLSADRIVIDPTT
                     AALGYGLEYGYSIMERARVGALQGDKMLSMPILGNVGHEVWRAKEANISTEDMPVWGE
                     QPNRGIIWEATTATALLQAGMNIVVMRHPKAAALVKQNIAELMVPTNFEA"
     gene            complement(15387..17294)
                     /locus_tag="VFC74_07205"
     CDS             complement(15387..17294)
                     /locus_tag="VFC74_07205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HZK25160.1"
                     /translation="MDKITVLFNPDNLAVQVEPGTSLLHAANLAGIELVSSCGGDGTC
                     GRCLVKVQDGHVRVKGTGNISSRAKQAGLVLACQTFAEGNVVVDIPGRSRLEEHQVLL
                     DEQQLLVEKDVEGAEGFKLQPLCSKLTLTLTPPTLDENVADWERLQTAVRNEICCSDI
                     RISLSVLKQLPEALRAGDWQVTIGVTQINGCAEVIAVESGHAKTSKLYGVAIDIGTTT
                     VAAHLVDLVSGFTVSKKGTYNKQARFGDDVISRIIHASERPEGLAELHHTIINTINGL
                     LAELLQPGNLAAQDVLMVTVAANTTMTHLFLGIPPKYIRLEPYIPLAAATPAVKAQEV
                     GLNINPEAYVVCFPSVASYVGGDIVSGTLVTNVAKDERITLFIDIGTNGEMVLGNQDW
                     LVSAACSAGPAFEGGGITYGMRAMKGAIDNVEINPDNFEVEVSTIGRCRAKGLCGSGL
                     IDCLATFRNVGIIDRAGKMQTDIQTPRLRQAEEGWEFVLVYGEDSDSGKDIVITENDV
                     KNLLRAKAAVYAGIHSMLNAVELDIGMIERVLIAGGFGNYLNIRDAITIGLLPDLPAE
                     KYMFIGNSSLKGARLALISQEAWHEAQELGDKITYLELSAGNDFMEEFMSALFLPHTD
                     MSLFPSVTNAK"
     gene            complement(17332..18663)
                     /gene="acsC"
                     /locus_tag="VFC74_07210"
     CDS             complement(17332..18663)
                     /gene="acsC"
                     /locus_tag="VFC74_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit gamma"
                     /protein_id="HZK25161.1"
                     /translation="MALTGLQIFKQLPKTNCKDCGNPTCLAFAMALAAGKTTLDKCPH
                     VSEEAKEALGAAAAPPIKLVKIGADHTVVELGDETELFRHDKRFYHSTAVAILVEDTE
                     DIASRLASFNDLEFDRVGLHYVTDMVALQCSSGDAATFTAAAEQVAANTEKAFMLVCE
                     DVAVMEAAAAAVAVRKPLLYAATNDNYEAMTALAQKVACPLAVKGNGLNDLAELVDKI
                     TKLGYKELVLDSGNRETSQVLADLTQMRRLAVRKRFRSFGYPTMAFTSKEDAHEEVVQ
                     ASVYVAKYAGLVVVKASKKEEILPILTWRQNLYTDPQKPIQVEAKVKAIGDVTPDSPV
                     YITTNFSLTYYIVEGEVENSKVPAYIVPINTDGISVLTSWAAGKLTGEAIAKALQENE
                     MEAKVNHKTLVLPGYVAVLSGSTEDASGWKVLVGPREATGIPSFAKEHFAN"
     gene            complement(18756..20939)
                     /gene="acsB"
                     /locus_tag="VFC74_07215"
     CDS             complement(18756..20939)
                     /gene="acsB"
                     /locus_tag="VFC74_07215"
                     /EC_number="2.3.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA decarbonylase/synthase complex
                     subunit alpha/beta"
                     /protein_id="HZK25162.1"
                     /translation="MSTTAFDQIYADAIPAGTKEPTKLLQRATNGAIIATSYAEILLN
                     QAIRQYGPNQPVGYPDTAYHLPVISSLSGEKVKTLGELTPILNRMRNQINSHLNFANA
                     RLCGESTAYAAEIIEALRYLNGAQPHVEPWTGYVSDPILRKFGIQLVDWTIPGQAVIV
                     GKARTNADAVKIVQELQAKGIMIFLVNEVIEQLLEEGMKLGVDYIAFPLGNFTQVIHA
                     VNFALRAGMAFGGIAPGLREEHRDYQRRRIRAFVMQLGEIDDVQVAAHFAAIFLGFPV
                     ICDTELEEEIPDWYVSHTDYDTLVKYCLELRGIKMKIVDIPVPITIGPAFEGETIRKG
                     DMYVEMGGGRTTAFELVQMVSEDEIEDGKVTVIGPEIDQFAEGARLPLGIMVKIFGRK
                     MQPDFESVLERRIHYFINYGEGLWHVAQRDLCWLRISKEAVAKGFQFKHYGELLIAKF
                     KDEFPAIVDRVEVTIITEQAEVDKSIKVARESYSLRDARLQGLTDESVDEFYSCILCQ
                     SFAPNHVCVVSPERVGLCGAVSWLDAKASFEIDPNGPNRPIIKEGTIDEEKGMWQSVN
                     EYVYGASNNSVEEVNLYTLMDRPMTSCGCFEAILALVPEANGLMVTTREHFGETPCGM
                     TFSTLAGAVGGGLQAPGFMGIGRTYMLSGKFIQADGGIARIVWMPKELKEYLGDDLRK
                     RAEDAGLGADFVDKIADESVGTTAEEILPFLEEKDHPALTMESII"
     gene            complement(20971..22941)
                     /gene="cooS"
                     /locus_tag="VFC74_07220"
     CDS             complement(20971..22941)
                     /gene="cooS"
                     /locus_tag="VFC74_07220"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HZK25163.1"
                     /translation="MTDVAKKKKPADRKRAIDPAVLEFLAKELPVDTAYDRYLAQQPQ
                     CKFGDTGICCRICIQGPCRITPKAPKGVCGATAYTIVARNLVRSVTGGAAAHSDHAKH
                     ILLALKALLNGQAPDYRIKSPEKLHAVAARFDIPTAERTDEEILADIVALGMDDFMRI
                     DGQKSKWVEKTITPGRLEKFNECNIMPTSIFEAIAGATAQTHMGMDADPVNLIFKALQ
                     TALADYAGEHIGTDMSDVLFGIPQPVYTEANMGVINPAKVNIAVHGHNPLLSEMIVMA
                     ARDLEDEAKAAGSAGIQLMGICCTGNEVLMRQGVPLVTNFMSQELPIMTGAIDAMVVD
                     LQCIMPGIQAVAECFNTRIVTTTESVKIPGAHHVDFSEENALEQAKSVIRIGIEGYKD
                     RLGNECTIPQIKNKVVAGFSVEALYDLFGAINAEAPIKVLTDALKSGELRGVALLCGC
                     NNLKVKHDDSHMVLAKELAKNDVLLVATGCAAGAYAKLGLLSAEAVELYAGEGLKAFI
                     HRLNEANADQLKGKLPLIFHMGSCVDNSRSMNLATDIANDLGVDTPKIPYVVSAPEAM
                     SEKAIAIGAWNVAMGLPVHVGVVPPVTGSELVNGVATQIAHDVYGGYFLWETDPQLAA
                     QKLLEALDYRSWKLKVHAQTSEKYDAAASTAW"
     gene            complement(23008..23784)
                     /locus_tag="VFC74_07225"
     CDS             complement(23008..23784)
                     /locus_tag="VFC74_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HZK25164.1"
                     /translation="MTLKIAISGKGGVGKSTFAANLIRTFANRGFNVYAVDADPDVSL
                     GTTLGLDPAALESLKPVIEMKAIVDQRSGGGSGALYSLNPKVDDLIDDFTIQQDKIKF
                     LRMGAVKQGGTACYCKENSFLYAVLSSLLLDKNDLVVMDMSAGIEHLTRGTSRGVDML
                     IIVTEPTRVSVQTTRVVQRLANELGIKQVKVLVNKVRTEKEKQFIANEFAQSELLAMV
                     PFDEGVLENALAEESGLLSGSGIMPGVDKIADYILQEVRR"
BASE COUNT         6611 a         6306 c         4973 g         6497 t
ORIGIN      
        1 tattaatggc ttccgccact gtagcaacgg cactgatttg cagacctgca ctattttgca
       61 gacctgccag gctaaatttt ggcactatgg cccttttaaa gcccagacgt gccccttcat
      121 gcaagcgctg ctccaaatga gggataccgc gcacctcacc agccaaaccg atttccccca
      181 aaaacagcag ttcccgtggt accgcctgat ctcggaagga acttaccaaa ctggctgcta
      241 cagccagatc tatagctggt tcaaccaatc taaccccccc ggcagcatta acaaaaacat
      301 caaaatcttg aatatgtacg cctacatgcc gctctaatac tgctaagatt aaggccacgc
      361 ggtttagttc cacccctgtg gccgtacgac gcggattacc atagcgagtc ggagtaacca
      421 gcgcttggat ttccaccaag agtggacgtg tgccctccat ggaagctgta actacagaac
      481 cggcagcatc aagtaaacgc tgcgaaagca gccactccga agggttttcc acttctcgca
      541 agcctgccgc atccatcgta aataaaccta tttcatgggt agaaccaaag cgatttttaa
      601 cggcacgcag aattcggtag agatgctgtc tgtccccctc aaaataaagc acgcagtcca
      661 ccatatgttc aagcacacga ggtccggcaa tttgaccctc ttttgtcaca tggccaatga
      721 gcaataccgc aatattctct cttttagcca aacgtaatag cgtagtggta acctctttaa
      781 gttggctcac gctgccagca gctgaagaaa gatgtgggct gtagactgtc tgcacagaat
      841 caacaatcac cacatcgggc cgtaatcccc gcacctgctc ttctaggata ctcatatctg
      901 tttcgggcaa taccataatc tgctcactgt ctacacccaa acgtttagcg cgcagcaaaa
      961 gctgtgcggg cgattcctca ccggcaatat agagaacctt cttgccagca ctccctaaac
     1021 cttgagctgc ctgcagcact agggttgatt tcccaatgcc cggatccccc cctatcaaca
     1081 ccaaggagcc gggtatcaag cctccaccca agacgcgatc taattcacga ctggctgtag
     1141 tcaagcgtcc cgcctgttca gtatcaccaa tagctgataa tggtaccacc tttgccgcag
     1201 agctgcgccc tactgaggcc ttcgctggtt ggcgcacctc ctctaccatc tggttccaaa
     1261 ccccgcagcc tggacatttc cccagccact ttggtgactc atagccgcat tgtgagcaat
     1321 aaaaaactgt tttacttcgt gccaagttct caccccgttc tcttcatcac tagcctatgt
     1381 gatttcttgc tgctcgtcaa ggttagatca agccaaaaca aacaacactc ctttagtgct
     1441 aaagaagtgt tattgaccga tcttgttaaa agttggcgga tttagttgtc ctaatttgtt
     1501 ttttacagca agcgacaaaa gaaatagtat tgcctgggca atatgccaac cgtagtttat
     1561 tttcgacaaa aatacttgat agttgacggc gctatttatt tacggctgcc aggctttacc
     1621 gcaggagttc cttgtttaca caacgggcag tcttctgcgt cataggatgc gacatcaagt
     1681 ctgattaacg cctgataagg aacgccaaaa tctactttgc cgttacttcg atcgacaatt
     1741 gaagctacac ctactacatt accgcctagc tcacggatca gcgctatcac ttcgcgcacc
     1801 gagccgccag tagtcaccac atcttcaagg accagcaccc gctcccctgc ttcaatgcta
     1861 aaacctctgc gcagtgtcat taatccagcc tggtcccgct cagcgaaaat accgcgtgtt
     1921 ttgcaggcac gtgccgtttc ataggcaatt acgaccccac ctaaggccgg gccaatgcag
     1981 acatccactt gctcttcttt aaacattgcc gccagggcct gacaaaactc ttctgcataa
     2041 tcagggtact gaaaaacgcg ggcacactgt aaataacgat cactatggcg gcctgaggtt
     2101 aaaagaaaat gcccctctaa cagcacacct gttttttgta agacgtctag tactctctcc
     2161 ttgctaatca tctatcctac ctccttgggg tcagatttac tgctcaaata agctctgcca
     2221 gtaaacgttt tgctgcttgt tgagggtcag gcgccgcggt cagcggacga ccgataacta
     2281 aataatcggc accctgcgcc agtgcttcgc gtggtgtaac aatgcgctcc tgatcattgc
     2341 tagccgccca ggccggacgg ataccaggcg tgataatcaa aaaatcatca ccacaggctt
     2401 gacgaatcca gggtatttca cgagcagatg ccaccacccc atccatgcca gcttctttag
     2461 ttaagctagc caaggcagcc acttgctcgg gcaaagctcg ctgcacccct aattcttcct
     2521 gcagttcagt ctcagaaaga gatgttaaca cagtaacggc aattaaaagc ggtgcaggaa
     2581 cacctaaagc agctgccttc tttttagccg cctgcgccgc tgccaacatc atctttttcc
     2641 caccaccagc atgcacatta aacataaaag cacctaaacc cactactgct tctgcagccc
     2701 gggccgtagt gttagggata tcatgaaact ttaagtctaa aaaaacttta cctcctgccg
     2761 acctgatttt ttttacaata ctggggccga cgctgttgta taattgtaaa cctactttaa
     2821 aagcacctac atagtcatgc aaagcctctg tcagcgctaa agccttctct tctgcatcaa
     2881 catcaagagc cacaattatt ttactgtgca ttttttgccc ccttttcttg tttttactcc
     2941 tggctggtta atttagccag tcccacaatt tcctgcacag actgaaagtg gtgacgtttt
     3001 aaatattccg caatgccggc tgctacttct ggacaaacgg tagggttaat aaaattagcc
     3061 gcgccaatcg ctactgccgt ggcaccagcc aacaaaaact caatggcatc agcagtgcag
     3121 ctaatgcccc ccatcccaat gactgggata cgcacagctt cagtcacctg ccacaccata
     3181 cgtagagcta caggtttaac cgccggaccc gaaagccctc ctgtcaaatt tgccaacacc
     3241 gggcgtctct tctctgcatc gatggccatc ccaactagag tgttgattaa agataaggca
     3301 tcagcaccgg cactttctgc ggcctgtgct atagcaacaa tatctgtaac gttcggtgac
     3361 aattttacaa taagcgtttt ttccgtaacc ctgcgcagcc gtttaatcaa ggctgccaca
     3421 accgccggat ctgtgccaaa ggccagcccc cccgcctgca cattaggaca ggagatatta
     3481 acctcatatg ccgcgactgt tggctcctcc tctaatgctt cgattacatg ctgatagtca
     3541 ttactgctat gacccgccac attaacaata actggcgtct tgtaaggtgc caattgtctt
     3601 attttttctt tagccgcggc aacccctgga ttttgcaaac caatggcatt gagcaggccg
     3661 gctgaagttt ccacaatccg cactggggca ttgccgagac ggggctctac ggtagtaccc
     3721 ttaaccacta tggctcctaa cttggctaaa tcataaaatg gtgcatattc ttcgccgtag
     3781 ccaaagcagc cggaagccgg catcacagga ttacgcaaag ttagctgccc cagcttaaca
     3841 gtcatgtctg gcgtcattcg gcaaacacct cctctgcagc aaaaaccgga ccgtccacac
     3901 agacgcggac ataggtaccg ttagtgctgg gaatcacaca ccccaggcag gcaccgacac
     3961 cgcaggccat ctgcgcctct aaggaaacat agcagcggca ctgataaccc gcagccaact
     4021 ttgctactgc acgcagcatg ggttccgggc cacaagcata gatatctgct ttatcggtca
     4081 gcagtgctgc ttgcagcaag tcggtgacta gacccttacg gcctagggag ccatcatcag
     4141 tagcctcaac ataagaggtg gctagagcct ggaactgttc gcgcaaaacc agctctgcag
     4201 cacttctcgc gccatagaaa aatgtaactt ctttgcctgc agcttgcagg ctgcgcagta
     4261 agaagaacaa tggtgccgcc ccaataccac ccgcgacaac gatagctggt cgctgcccac
     4321 caagggggaa gccttgtccc aacggaccta ctactgttac tgattcgccc accttttttg
     4381 ctgataacaa acaggtacct cgtcccgcga gccgatagag cagagtcaac tcacctgctc
     4441 gccaatcatg tacacttaaa ggacgcttaa gaaaaggatc ataggaggtt gttacgccaa
     4501 cctgtacaaa ttgtcccggc tgggcagtaa tttcgcccgc caacgttaac cggcgcactt
     4561 caggacttac tgcttgatgc ttgataattt ccatttctat tttttctggc atcataaact
     4621 tcctaactta attaattttt atctctccgt ggtgcatgac tacttttcct tccaccatgg
     4681 ttaagactgg caggccatag acaagccaac cgtgaaaagg agtattgcgg ctctgtgaat
     4741 aaaaaagctc cttatcaata acctgctgct gctctagatc aataatggtc aaatcagcag
     4801 caaaacctgg ctttaaggta ccgtgtggaa tgtccagtag tgctgctggc cgacaggaca
     4861 ttcgttcgac taaaccggat aatgtcagaa gttcggttct aactaggtga gtataaagca
     4921 gggggaaagc tgtctccaaa ccgataatgc caaatggtgc taagctaaat ccacgttctt
     4981 tatcgctcgt ggtatgtggt gcatgatcag tggctattaa gtcaatcgtg ccgtccacca
     5041 gggccgtaat cagagcttgg cggtcagctt cggtacgtaa aggcggtttc atttttgcca
     5101 gtgtgccgta tttaagcaca gcttcttcag agagtagtaa gtgatgcggt gttacctctg
     5161 ccgtcactac cacccccgct gttttcagca gtcgcagcca gtcgacagtt tccgcacaag
     5221 acacgtgcat aatatgtaag tgagctttac attccttggc cagcatagca tcgcgtacca
     5281 tcatcagcgt ttcagaactt actggaatcc cgggcagttg taactgtttt gcaactttcc
     5341 ccgggtggat ttgaccactt cccgcaatac tttcctcttc acagtgctca cagtagggga
     5401 tcttcagttt ggcacattcc tgcaaagccc gtcgagccag ctctgcctgc tgtactccgc
     5461 gtccgtcatc agttactgca cgaatacccg cctgtaccat ttgtgcaatg tcagtcaatt
     5521 cttggcctgc agaatgcttg gtaatagccc ccaagggcca gatgcgcaca agactatctt
     5581 ctttaatgcg ctgccataat tgctgcacct tttgctgatt atcgcataca ggctgcgtat
     5641 taggcaaagc agtgatacta gtataaccgc cggcggctgc ggcacggctg ccggtacgta
     5701 ttgtttcttt ttcctggccg ccgggttcac gtaaatgtgt gtggacatcg attaaaccgg
     5761 gaataacagt acggccctca cattctataa ctaaccgtcc ccttgtagca atgccttccg
     5821 ccagctcaac tattttgccc tcacgtatta aaatgtcaca attcccatga tatagctggc
     5881 tgggatcaac cactacacca ccacgcacta cagtttccaa gaccccaccc cctcgctagc
     5941 aatctttttt atcaataaag tgaaatgaaa acaaattccg tacgtagtgc attaagtccc
     6001 gtatctagtt ctagatatcc cgtcagtttt ccttttttct ggcaggctaa gaaaatggca
     6061 ctaggcagaa aaagacgcaa ctaaggcgtc tttttacggc agaaaggccc tgtaccacag
     6121 atgtagttac taaacagcag gtagaattat tttccgacct aatttgtatt ttctaaataa
     6181 agggtgaaaa ataatctcat ggttaatacg ccataggctg ttttgatact gtagtgccat
     6241 tttcatgcag ttttctggga aacgctcata acgtaacagc ggggcaacct gatagtagaa
     6301 atcaaggcct tcttttgtca gggttaaggt gtccttttcc agaagatcac ctttttttaa
     6361 atctgttagc aagccctgaa tatcaaagct ataaacgctg acagtaccac ggtagaaatc
     6421 ataacgaatc ccagcacgtt cagcggcggg agtattagct gcggccaaaa agagaaaatt
     6481 atgcagcgta cggctacgat caaaaggaaa attggcaatt agctttagta ataagatgtg
     6541 gtgcaaagta taggtaaatg aatatacttt caagacaatg gccccttttc tttgtctaga
     6601 ctctcttttc attttggcta aaaaaatata tattatgcct ttctccctcc atggcctcgt
     6661 aatattgggt aaataaataa ttctctagtt tacgcgccag acgaagacgg cgacaaacaa
     6721 gacgatagga ctgtttgata gttaagcgat gtccaccttt tagaatcaag gtggaaggtg
     6781 caggtttggc cagtacttca agcagcgaac gcagcttgaa gtagccatag gcacagtcac
     6841 ctttaacgct aggcactctg gttttaagag gcacgaaaat gataatggga gataaggcca
     6901 aggggacaag gttcttgcag ccgatgattg gccctaaccg tttgcgcatt tctgataagt
     6961 tcagtaaata taatttagct agctgctgta gaaaagatcg taagcccact gctgctacac
     7021 tagtctcacc atcacgaaag tacagttgta cacactggcc aatgccggtt ttatctatgg
     7081 gcaagaatgc taccattctt tgcatgtcac gaatttgcat caaaaaacct ccttactggt
     7141 atgtagggag gttccttcat cttatgctgc cgcgttggct tgtgacgcct taatgttccg
     7201 gctcaccctg caggtaggaa agcatattct ggtacgctct ttcctcaagc tgcaaagcat
     7261 gattaatttc tcgaaactta ttagagatac tagcacaaga aacgccatat tcggccgcca
     7321 attctttttg cgttaagtcc aaaaaatgaa aacgagctaa actatattct aaggcggcgg
     7381 cccaagtctg tactttgctt atgcgcggtg tgtcagggta gacagtgttg ataaagtcca
     7441 gccagatcgc ttgcacatcg gcaagaaagc cttcatcata gcaattactg gcacgcatgc
     7501 tgccgatcgt acagtcaatt acttgctgcc attcagggcg ccaggtaggg gtacgtaacg
     7561 gatatttagc taaatctact tcccttaatt gtcccgctga aaatatctgc actttactct
     7621 cgcccaggtt ttttaattcc aaaagggcca tttgtcgaaa gcgctcttta acccacgggt
     7681 ttctgacaaa cttacgcaat gctctgcagg ccactttgcc gcctaattta ccataaatgc
     7741 tgagaatatc ttccttatac tggtccgtgc gagcatataa catactatcc agcgcccggc
     7801 gatatctatt gttattaaga taatgcgccg ttaattcttc agtcttcgct gttgtgatga
     7861 tatcctccag cacaaagtct ggttgggcat aagtcggtaa ggtgtacaaa tcatcatcat
     7921 cgtcaaaagc atccagcagt tctgctgctt ccagcgatag ctcaccttcc ggatctgtct
     7981 gcaaataacg tagtaaatat tcgcgtgatt tttccatctc atccagtagc ccgtaattta
     8041 cagccaacag aaaatagcag tctgtcattg tttcatctaa acgattgatg atatgcaaaa
     8101 agacacggtt agcttctttg agctgaccct gcttactaag catacaagcc agattataat
     8161 ggttgaaggg gttttgtggc tcaacactca ctgctttttt aaagtattgt aaggctttat
     8221 cgtgcttatt cttttggtaa tataacatgc ccttgcgcaa ataaaaaccc gcctcgcgtt
     8281 caaaggggac taccttttct ctgtttatgt cattcccagt tcccttggta ggatttttca
     8341 tgttttcatc accaaccttc attcagcctt acccagataa atatcccgtg ccaacaaacg
     8401 atcgtattcc gtccaattat tactatgttc accttttcgt gaaacttgtg caaattgatt
     8461 aagacgtgcc gaaattaaat cagcatgatg cagagcatag gcagcgaaag tacgggggat
     8521 ttctggcgaa ccccattcct tttgaccatg atgggcaagg attaaatgca ccagctccat
     8581 gtgcaaatca gggggaaaat caacaatatc tttaattttt gcttctagca tatcttttcc
     8641 cataataata tgccccaaaa gttttcccgc tgtggttagt tctatcgaca tacccttcac
     8701 ttcatattcc tccaacttac caacatcgtg cagcaacgcg ccgcaaagga ctaaagacaa
     8761 atctaactgg gagtgcagct gcacaaaatg ctgacaaagc gccgctactt ccagagaatg
     8821 ttctaaaagc ccgcccacat aattgtgatg tactgtacgg gcagcaggag cctgactata
     8881 gcgctgtaaa aacgcttcgt ctgcaaaaaa tgcttgtaat aagctatgca aataagggtt
     8941 ggtgatactg ctaataagag actgtaattc gcctaccatc tcttcacagc tgcgaggagc
     9001 aaccttttga aagtgctcat attttatttc tgccagtggc acaggttcta aactatagat
     9061 aataagctgt gaaccacgat aattactaac ctcaccacgc acgcgtacta catcaccaac
     9121 ttcaaataaa gcagccagtt cagggccttg ctcccaagct acagcacgca tggtacccgt
     9181 gctatcggcc acctgtaaac gtaaataata ttcgccagcc cgattaggct gtgaaaaagg
     9241 gtttaatgct ttttctgtga cggcaaaatc actttgcaca gcatcgccca ccttgagctc
     9301 actaataaat tgcttttcca ctatttcctc cattaaatca gatagtaatt aatgttttct
     9361 ttattatatt ctattcccca caagcagcaa atttcctctt tccacatcac atcatgagct
     9421 tacaaagtga cattgccctg gagattagtg taggggcgaa atgagttcgc cgcacaagaa
     9481 agacgcttcg cgtttctttc aatgatgcgt ctttcttgtt ctttaggggt aggcctaacc
     9541 cagtgagcgg tagcgaacgg agttaaagta gccaagtctg ctaaagaaat tacatataac
     9601 tattcgctca tactttgggt tagctgattt tctagcaatc gcctgccacc ccttcgacgg
     9661 acctgcgggt cctgaccacc ccgcaagatt aagggggaat ccccctgaaa actcccctca
     9721 tcggatagaa aaaaaaattc aaggttaaca taatttcctg tccgccaaaa taaataagcc
     9781 tgaccagaag gccaggctta atgttaatgc agaattttag tcgaatgtgg gacgcggcag
     9841 caaacctgcg cgctccgcaa tctctggtac gatttcttcc caggcaaccg ccatgatgtg
     9901 aacaccggca acaccttcaa tagtcttgag atgttcaatc atttcgatag ccatatttac
     9961 accctcagcc tttttatcct tagcaccttt tagacgatca ataatatgat ccggtacaat
    10021 catcccggca acaaatttct tcatataacg tgctgcacca agggatttaa gaggggtgat
    10081 accagcaata atacgcactt tttcatgcaa gcccatatcc actacttttt tcatgtagag
    10141 ctcaaagcgt tccatatcaa aaataacttg cgtttgaata aagtcggcgc cggcggcaat
    10201 cttcttcgcc aagcgatagg gacggtattc aaaggggtcg gcaaaggggt tagccaccgc
    10261 accaatataa agatcgatgg gcgcatccaa cttatcgccg ccaataaatt cctgctcatc
    10321 gcgcattttt ttcagcgtgc tgatgaggtt catagaatca aggtcattca catttttcgc
    10381 ctgtggctca ctgcctaatg aagcatggtc gccctgaatg cagaggacat tcttcattcc
    10441 cagcgccgct gctcccagta catccgcctg aatagcgata cggttacggt cacgcacagt
    10501 catctgcacg tttggttcaa tacccatgtc gagaataatt ttacctgcag cgatactaga
    10561 ggtacgcaca atagcggttt ggttgtcagt aatgttaact gaatcaacac aacctttcag
    10621 gtggcttgca tgatgttcaa taacttcacg ttccccgctt tttggaggac caagttcacc
    10681 tgtaaccgca aatgctccgc tttccaaaac tttttgcaaa ttgcttttag tcattacagc
    10741 ttcacatcct ccctgatcat tttacgcggg ccgccattgt gggatgttga ccaatcttta
    10801 ggttctttga tttcttgtaa agcatccaac ttattaaatt tcatcagccg ttccacaatt
    10861 aaatgccagg cgcaatctaa atctttattt acttcgcatt tgccattctg cgaaccacca
    10921 cagggaccgt ttaacatgct cttggagcag cgagcaatag ggcaaatacc catcgtttca
    10981 tccaaaacgc agttgccgca accgaggcag ttttccatcc attcaccctg tgctgtgggc
    11041 atacccataa aggtagtgtt cacaccagga agagtacgtt tatcgtcaaa atacatattc
    11101 atgttctgga taccaacacc acaagccaaa gagacaacaa catcagcttc ttctactagg
    11161 tggcggacag attccaggta ttcgggttca cattggcgct ctactgtatg agttttaacc
    11221 tctatagggt tgcctgcttt attacgggca atggaaagag ccaaggctgt ttcacgcgct
    11281 tctttatctc caccagccat acagacagtt acacactcac cacagcctaa caccagcact
    11341 ttcttgaagg gctcaaggtt agctaatatc tctttaagtg gtttacgttc agcgacgatc
    11401 atttactctt cacctccctc tccgtttaag acagcgtcga cttttttcag atacttttcg
    11461 gcaacatttt taaagtctct tacttcatct accgataaag tggcgtactc aacacgctct
    11521 ttttctaggc ctaattcagt caagatttcc tgagcacggc cagtacgttt agtggcccat
    11581 ttatcaacat cttgataggt acactcatct tcctcttggc agccggcaac ataaacagcc
    11641 tctgccccaa gattaaaggc attgagaata tgttccactt ctactttggc cacgcagggt
    11701 acgcgcacaa tagccaggtt ttgtggtttt tcattattaa cgtaattgag gaaagaacgt
    11761 tcagcaaaag cgccataact gcagcagaag actactgtag ccggtccacc attaccttga
    11821 gccagtttaa cggcatcctc tagttgatca agagcatgtt gggcatattg gtcacggaaa
    11881 cgaatagcat gagctgggca gataccaacg cagagaccgc aagcctgaca ctcttcatta
    11941 cgaatagaga agacaccatt ttcatcaatg gtcggaacac catagggaca aatgcgcaga
    12001 caagtgagac aaactgcaca aagctcgtct aaacattcag cacctgcggc gcagtccagg
    12061 cagcgccgac tttcacaaag tgccgcacgc tcatcatagc ccacttcaac catatcaaag
    12121 tgttgagtcc gtgtttcttt atctaaattg ggtacttctt gacgggcaat gcgcttcact
    12181 ttttccacag tacttgcagc cagcggctcc aaagtagtaa agcgatctac aaagtctgtg
    12241 tcaaaaggca catcatctaa ataggcgtta actgcttgtg ctactttttg tcccgaagct
    12301 aaagcttcta ctgccaaacc gggaccaaaa gctacttcac cagcaacaaa tacatcagga
    12361 cgcgtagtct gcagggtgtg tctgttgtat ttcaagacac ctcgttctgt taattccacg
    12421 ccggatgtag tcaaatagga caggtcgtca ttggcttgac caatagccag aacaactaca
    12481 tcacagggaa ttaccttctc attggcacca tattggggat taaagcgttt ttgttcgtct
    12541 agaacagaaa gtacttcttt tacttctaca ccagtaactt taccgttatc atcaacgaga
    12601 atttgctgct gaccccagcc tggatgcata acaatgcctt cctctaatgc ttcttcaatt
    12661 tcccaagagt tagcaggcat ctgatcatgg cattccaagc acattaattg gacactttct
    12721 gcaccaacgc gaactgcaga acgtgccacg tccatcgcca cgttaccgcc accaataact
    12781 aaaacattgc ggcctggctc gaatttaaaa tcatcaaatt taacactgct taagaatggt
    12841 aaggctaaca ttacatcttt agcatcagca ccaggcaggt tcaaggaacg gctggtcggc
    12901 agccccagag ccagaataat agcttgataa tctttagcta attcatcaag ggaaacatct
    12961 ttacctaact ctacgccagt tttgatttcc acaccaagag cggctacccg gccaacatct
    13021 ttccgtacag cctccatcgg taaacgataa gtgggaataa agttagtaat tgcgccacca
    13081 gcttcagagc tgcgttcaaa tactgttacc gcaaaaccca tttcagctag atctttggca
    13141 gctgctagcc cagctggccc accacctacc accgccactt tttcactacg tgttacttct
    13201 gcttttttaa cttccggcca agcaccattt tccagagcaa aacgcttcag agcgcgaatt
    13261 gatacggcct catccacttc tttgcgccgg cactccgtct cacaaggatg tgcacagata
    13321 ataccgcaca ctgaagcaag tgggttcgga gctgtgattg cttctactgc tttatcataa
    13381 ttacccacag agatctgccg aacatattcc tgaacatcgg tatgaacggg gcagccgccc
    13441 tgacaggggg gatactcggg tacagcagaa gacaagtcat acttgatatt actcataaga
    13501 atcttcctcc tttcagctat ggtttctatt tctgattttt atatatgtca acgtagctgt
    13561 cacaataaat atccaggttc atgataactc tggcagtggc gataattgcc attaaatctg
    13621 cgtcacaagc atcaccgaca gctgcgtcta gaccattagc aatagccatg gcagcaaatg
    13681 ttttattgat cagggagcgc cctcccttgg tgccctgaga gatattggag agtcctacca
    13741 ctgtacgcgg cgccgggtcg gacagtaact taacctgctg cagggtacgc attacttcag
    13801 gcccatggtc ttggcccaca ttgcagggca aaactaaagg gtcgatcaac aaattttcca
    13861 tggggatttc ataggaatct gctgccgcca ccaattccat agccagtgcc acacgggtat
    13921 cggcatcttt ggggatacct tcctcattca tggccaaacc gatcacagcc gcatcatact
    13981 ttgcggccat tgtaaaaaca cgttccattt tcggccattc cgctggcaca gagttaatca
    14041 ttgctggccg tttgcacact tctagaccag cttcgatgac atcatagttt gtcgaatcaa
    14101 ggcaaagggg taaatcactg gcttcttgtg ttacttctac tagccagcgc atagctttaa
    14161 tcggatcgct agctgctggt cccgtattaa tatcgagata gcgagcacca gcctcatcct
    14221 gccggcgagc ccaatcctga acagcacgcg ggtcttgttc gcgaatagca tgagcaatgt
    14281 ctttaaacat cccattaatt ctttcgccga taataaacat aattaaacct ccctttgttt
    14341 aaagtcagca gacaacaact acttacgcca ttatcaactt attaggcctc gaagttagtg
    14401 gggaccatta actctgcaat attttgctta actaaggctg cagctttggg atgacgcatc
    14461 accacaatgt tcataccagc ctgcagaaga gcagtggctg tagtggcttc ccaaataata
    14521 ccacggttag gctgttcgcc ccagactggc atatcttccg tagaaatatt tgcttcttta
    14581 gcccgccata cttcgtggcc tacattgcca agaataggca tagacagcat tttatcgcct
    14641 tgcagagcac caacccgagc acgctccata attgaataac catattctaa accataacct
    14701 agagctgccg tagtgggatc aataacaata cgatcagcgg ataaattcat ttctgtgatc
    14761 aggatattaa gctgcttaca gatattaatg tcgatgggag agttagcaat caaactgtgt
    14821 ttgtgcacca tgcaggcagc agcaattgat ttataatttt cctgttcagc agaaccaatt
    14881 aatagatttt caccagctgt tgcttctgct accgcaggca gcacctgatt atctttttct
    14941 gctttaccac aaccaagtac aattaacgga cagggaacag cagctagaac atcctgcaca
    15001 actttaacac aatcttctgg agaggcatca gcgatgtcgg gatctgcccc tttgagcctg
    15061 acaacaagca catctgcgcc atattcctca acacatttct tagcccactg ggtagggtta
    15121 tcaaaaacgt caccatagta tggcgtgaaa gattcattcc agtcagtcgg cgcaatatcc
    15181 cagatctcta aagctacgac gggacggttt ggtatttctc cttcaaaatg taggaagggc
    15241 agcgcacttt caccgccaac agtaacggtg taaccacggg taccgccttc ttcttgggtg
    15301 gcgcccaata ccactgtatt aactttattt ctatactttt ctttcattgc actgaaagcc
    15361 atttggttac gctcctttct atccggttat tttgcatttg ttaccgaagg gaacaaagac
    15421 atatctgtat gaggtaaaaa gagggctgac ataaattctt ccataaaatc attacctgca
    15481 gatagttcca aatacgtaat cttgtcacct aattcctgcg cttcgtgcca ggcctcttgg
    15541 gaaattaaag ctaaacgtgc tccttttaac gaagagttac cgataaacat atatttttct
    15601 gctggtagat cgggtagtag accaatcgta atggcatcgc gaatgttaag gtagttgcca
    15661 aacccaccgg caatcagtac tcgctcgatc ataccaatat caagttcgac ggcattgagc
    15721 atactatgga tgccggcgta aacagccgct ttagcgcgca gcaaattttt tacatcattt
    15781 tcggtaatca caatatcctt gccgctatcc gagtcttcac catagaccag aacaaactcc
    15841 cagccttctt cagcttgacg caaacgtggc gtttggatat ctgtttgcat tttaccagcc
    15901 cgatcaatga taccaacatt acgaaaagta gctaaacaat ctatcagacc agaaccacaa
    15961 aggcctttag ctctacaacg accaatcgtg gatacttcaa cctcgaagtt atcagggttt
    16021 atttctacgt tgtcgatggc gcccttcatg gcacgcatac cataggtaat gccgccacct
    16081 tcaaacgcag ggccggcaga acaagctgct gagaccaacc aatcttggtt gcctaacacc
    16141 atttcaccgt tagtgccaat atcaatgaac agcgttatcc gctcatcttt cgcgacatta
    16201 gtaaccagcg ttccggaaac aatatcgccc cccacatagg aggccacaga gggaaagcaa
    16261 actacataag cttcaggatt aatatttaac cctacctctt gtgccttcac cgctggggtt
    16321 gctgctgcca acgggatata tggttccaaa cgaatatatt taggtggtat tcccaaaaat
    16381 aggtgcgtca ttgtggtatt ggctgccact gtcaccatta gcacatcttg tgccgctaaa
    16441 ttgccaggct gcagcaattc tgccagtaag ccattgatgg tgttaataat cgtatggtgc
    16501 agttctgcta acccttcggg acgttcactg gcatgaatga tgcgcgaaat aacgtcatcg
    16561 ccaaagcgag cttgtttgtt ataagtgccc tttttactga cggtgaaacc acttactaga
    16621 tcaaccaagt gggcggcaac agtagtcgta ccgatatcaa tcgccacgcc atatagtttt
    16681 gaggtcttgg catgccctga ctctacagca ataacttctg cgcaaccatt aatttgcgta
    16741 acaccaattg taacctgcca atcacctgcg cgtaatgctt cgggaagctg cttgagtaca
    16801 gacaaactaa tacgaatatc tgaacagcat atttcgttac gtactgccgt ctgtaaacgt
    16861 tcccagtctg cgacattttc atcaagtgtt ggtggcgtaa gcgttaatgt aagcttactg
    16921 cagagtggtt gcaatttaaa accttctgcc ccctcaacgt ccttttctac cagcagctgt
    16981 tgctcgtcta aaagaacctg atgttcttcc aggcgtgacc tgccggggat atccaccacg
    17041 acattgccct cagcaaaagt ttggcacgct aaaactaaac cagcttgttt agcgcgggat
    17101 gagatattgc cggtgccctt gactctaacg tgaccatctt gcactttaac caggcaacgc
    17161 ccacatgttc catcgccgcc acaactgctt accagttcta tgcctgctaa attagctgca
    17221 tgcaacagag aagtgccagg ttctacttgt accgctaagt tatctgggtt aaacaaaaca
    17281 gttatcttat ccacgtggtg tcacctcttt tgtctgctag gttaggcaaa tttaatttgc
    17341 aaagtgctct ttcgcaaagc ttggaatacc agtggcttcg cgtggtccta caagaacctt
    17401 ccaaccagaa gcatcttctg tactaccgga taatacagca acataaccgg gcaatactaa
    17461 ggtcttatga ttaactttag cttccatctc attttcttgg agggccttgg caatcgcttc
    17521 accagtcaac ttgccagcag cccaagaagt caggactgaa ataccatcag tattaattgg
    17581 cacaatatag gcaggtactt tactattttc tacttcaccc tcgacaatgt agtaggtaag
    17641 cgagaagtta gtggtaatat aaactggcga atctggggtt acatcaccaa tagctttaac
    17701 tttagcttcc acttgaattg gcttttgagg gtcggtatat aggttttgcc gccaggtaag
    17761 aataggcagt atttcttcct ttttacttgc tttaacaaca actaatccag catattttgc
    17821 cacatagacg ctggcctgta caacttcttc atgcgcatcc tctttactgg taaacgccat
    17881 ggttggataa ccgaaggagc gaaaacgctt gcgcacagcc agacggcgca tctgagtcag
    17941 atctgccaat acttgcgagg tttcgcggtt gccactatca agcaccaatt ctttataacc
    18001 aagtttggtg attttgtcaa ctagctccgc caagtcatta agcccattac ctttcacagc
    18061 aagcggacat gctacctttt gggctaaagc agtcatggct tcatagttgt cattagtggc
    18121 ggcataaagt aaaggtttac gtaccgctac tgcggcagca gccgcttcca tcactgccac
    18181 atcttcacag accagcatga aagctttttc cgtgttagct gcaacttgtt cagcagctgc
    18241 tgtaaaagta gcggcatcac cggaactgca ctgtaaagca accatatccg taacatagtg
    18301 taaacctaca cggtcaaatt ctaaatcgtt aaaagaagct aggcggctgg cgatatcctc
    18361 cgtatcctcc accagtatcg cgacagctgt agagtgatag aaccgcttat catgacggaa
    18421 taattctgtt tcatcaccta actccaccac cgtatgatca gcaccgatct taactaactt
    18481 aatgggtggt gctgcggcag caccgagtgc ttctttggct tcttcagaaa catgtggaca
    18541 tttgtctaaa gttgttttcc cggccgccaa agccattgca aaagctaggc aagtagggtt
    18601 gccgcaatct ttgcagttgg tcttggggag ctgcttaaaa atttgaagac ctgttaaagc
    18661 cataatttta tactccttcc tttcctaaaa atgtcttttt caaccataaa caagttggta
    18721 ggcaggctgg ttcagcctca gcctgcctac caactttaga taatcgattc catcgtaagg
    18781 gcaggatgat ctttttcttc caagaaggga agtatttctt ccgctgttgt tcccaccgat
    18841 tcgtcggcga ttttgtctac aaaatcagcg cccaaaccgg catcttctgc ccgcttccgc
    18901 aaatcatcac caaggtattc tttaagttcc ttaggcatcc agacaatgcg ggcaataccg
    18961 ccatcggcct ggatgaattt gccagacagc atataggtgc ggccaatgcc catgaacccg
    19021 ggagcctgta agccaccacc gacggcacca gctaaagtgg agaaggtcat gccgcagggg
    19081 gtttccccaa aatgctcacg ggtggttacc atcagaccat tagcttccgg caccagtgcc
    19141 aaaatagctt caaaacaacc acaggaggtc atgggtcgat ccatcaatgt gtacaggtta
    19201 acttcttcta cgctgttgtt agaagctcca tatacatatt cgttaaccga ctgccacatc
    19261 cctttttctt catcaatagt gccttctttt ataatcggtc tattgggacc atttggatca
    19321 atttcaaatg aggcttttgc atctaaccaa ctaacagcac cacacagtcc aacacgttcc
    19381 ggcgagacta cgcaaacatg gttcggtgca aaggattggc acaaaataca agaatagaac
    19441 tcatcaacag actcatccgt taatccctgc agacgggcat cgcgtagaga gtacgattcc
    19501 cgcgctacct tgatagattt atcaacttca gcttgctctg taataattgt aacttctaca
    19561 cgatccacaa tggccggaaa ttcatctttg aatttagcga ttagcaattc cccgtagtgc
    19621 ttaaactgaa atcctttggc cacagcttct ttagaaatac gcagccagca gagatcacgc
    19681 tgggctacat gccagagacc ttcaccatag ttaatgaagt agtgaatccg acgttctaaa
    19741 acactttcaa aatccggctg cattttacga ccaaaaattt tcaccatgat gcccaggggc
    19801 aaccgagcac cctctgcaaa ttgatctatt tccgggccaa tgactgtaac cttaccatct
    19861 tctatttcat cctcgctcac catttgcacc agctcaaagg cagtagtgcg accaccgccc
    19921 atttccacat acatatcacc cttacggatg gtttcccctt caaaggcggg accaatggta
    19981 atgggaacag ggatgtcaac gattttcatc ttaatgccgc gcagttccag acaatacttc
    20041 acaagggtat cgtaatcggt atgcgaaaca taccagtcag gaatctcttc ctctaattcg
    20101 gtatcgcaga ttaccgggaa acccaggaaa atggccgcaa aatgtgcggc gacctggaca
    20161 tcatcaattt cgcccagctg catcacaaaa gcccgaatgc gacgacgttg ataatcacga
    20221 tgctcttcac gcagcccggg agcaataccg ccaaaagcca tccccgcacg caaagcgaag
    20281 tttacggcat ggataacctg agtaaaatta cccagtggaa aagcaatata atccacacca
    20341 agcttcatgc cttcttccaa taattgctca atgacttcat tgaccaagaa aatcataatc
    20401 cccttggctt ggagctcctg cacaatttta acagcatcag cgttagtgcg agccttaccc
    20461 acaatcacgg cctgaccggg gatcgtccag tctactaatt gaatgccgaa tttacgcagg
    20521 atagggtcgg acacataccc tgtccagggc tccacgtggg gctgcgcacc atttaaataa
    20581 cgtagtgctt ctattatctc cgcagcgtag gctgtagatt caccacaaag gcgagcattg
    20641 gcaaaattta aatgcgagtt aatttgattt ctcatccggt tcagaatagg tgttaactca
    20701 cctaatgttt ttactttttc gccagaaagg ctgctgataa ccggcaaatg ataggcggta
    20761 tcaggataac ctacaggttg gttaggacca tattgacgaa tagcctgatt taaaagtatt
    20821 tctgcataac tggttgcaat gatcgcaccg ttcgtggcac gctgtaaaag tttagttggc
    20881 tcttttgtcc ctgcaggaat tgcatcagca tagatttgat caaaggctgt tgtagacatg
    20941 ctctttcctc cctttcaagt aacgtttttc ttaccaggca gtagaagctg ctgcatcata
    21001 tttttcggat gtttgggcgt gtacttttag tttccaacta cgataatcca gtgcttctaa
    21061 aagtttctgg gcagctaact gcgggtcagt ttcccacaag aagtaaccac cataaacatc
    21121 gtgtgcaatt tgcgtagcaa caccgttaac cagttcactg cctgtaacag gaggtacaac
    21181 tccaacatgg accggcagac ccatcgccac attccaggca ccaatggcaa tggccttttc
    21241 actcatggct tcgggtgcac ttacgacata aggaatcttg ggtgtatcaa cacctaggtc
    21301 attggcaata tcggtagcca aattcatgga acgtgagtta tcaacacaag aacccatatg
    21361 aaagataagg ggcagtttac ccttaagttg atcagcgttt gcttcattca aacggtggat
    21421 aaaggcttta aggccctcgc ctgcgtacag ctcaacggct tctgcagata agagacctaa
    21481 tttagcatag gcaccggcag cacaaccagt ggccaccagc aaaacatcat tcttggccag
    21541 ttcttttgcc aagaccatat ggctatcgtc gtgtttaact tttaagttgt tgcagccaca
    21601 taacaaggct acgccacgta actcaccgga tttcagggca tcagtaagta ccttaatggg
    21661 agcctccgca ttaatagcac caaacaagtc gtagagtgcc tctacactaa aaccagcgac
    21721 tactttattt ttaatttggg gaatagtaca ctcgttgcca agccgatcct tgtagccttc
    21781 aataccaatg cgaatgacag acttggcctg ttccagcgcg ttttcttcac taaagtcaac
    21841 atgatgcgcc ccgggaattt taacgctttc agtagtagta acaattctag tgttaaagca
    21901 ttcggcaacg gcctggatac caggcataat gcactgtaag tctacgacca tcgcatcaat
    21961 agcaccggtc ataatcggca attcctgtga cataaagttt gtcactagcg gcactccctg
    22021 gcgcatcagc acttcattgc cagtacagca gatacccata agttgaattc ctgctgaccc
    22081 agcagctttc gcctcatctt ctaaatcgcg agcagccatc actatcattt ccgagagcaa
    22141 aggattgtgt ccgtgcacag caatattaac tttagcaggg ttaattaccc ccatgttagc
    22201 ctcagtatat acaggttgtg gtataccaaa taacacatcg gacatatctg taccaatatg
    22261 ctcaccagca taatcggcca aggctgtctg taaagcttta aatatcaaat taaccggatc
    22321 agcatccatt cccatatggg tttgggcagt agcaccagca atcgcttcaa agatactggt
    22381 cggcataata ttacactcat taaatttttc taaacggcca ggagtaatgg ttttttcaac
    22441 ccacttactc ttttgaccat caatacgcat aaaatcatcc ataccaagag ctacaatatc
    22501 ggcaaggatc tcttcatccg tccgctctgc cgtcgggata tcaaaacgtg ctgctaccgc
    22561 atgcaacttc tcggggcttt taatacgata gtctggtgcc tgaccgttaa gcaatgcttt
    22621 cagggccagc aagatatgct tggcatggtc agagtgggca gccgcgccac ctgtgacaga
    22681 acgaacaagg tttctcgcaa ctatggtata tgccgtcgca ccgcaaacac ctttaggtgc
    22741 cttaggtgtg atgcggcaag gcccctgaat acatatccgg cagcaaatcc ctgtatcacc
    22801 aaacttacac tgtggctgct gagccagata gcgatcatac gctgtatcga caggtagttc
    22861 tttagctaga aactctaaaa cagcggggtc aatggcgcgc ttgcgatcag ccggtttctt
    22921 cttctttgca acatcagtca tgcgtattga atacctccct tattagtaca taaagtcttt
    22981 tgtcctttct gcacaaaata catctcctta tcgccgcacc tcctgtaaaa tatagtctgc
    23041 tattttatct acaccaggca taattcctga tcctgataac aagccgcttt cttcagccag
    23101 agcattctct aagacccctt catcaaaggg caccattgcc agcagttctg actgtgcaaa
    23161 ctcgttggca ataaattgct tttccttttc tgtcctcacc ttattaacca atactttaac
    23221 ctgcttaatg cctaattcat tagcaagccg ttggacaacc cttgtcgttt gcactgaaac
    23281 acgtgttggt tccgtaacaa taatcaacat atccacacca cgcgaggtgc cgcgtgttaa
    23341 atgctcgatg ccagcactca tatccatcac cacgagatca ttcttgtcta gcagtaagct
    23401 gctaagcacg gcatacaaaa aagagttttc cttacaataa caagccgttc caccttgctt
    23461 aacggctccc atacgtaaaa atttaatttt atcctgttgg atggtaaaat catcaatcaa
    23521 atcatcaact ttggggttca aactataaag cgcaccgctg ccgcccccag aacgttgatc
    23581 aacaatggct ttcatctcaa ttaccggctt aagcgactcc agcgcagccg ggtctaggcc
    23641 taaagtagtg cccagactaa cgtcagggtc agcatctacg gcatacacat taaaaccacg
    23701 gttagcaaat gtccgaataa gattggcggc aaatgtactt ttgcccacgc cacctttacc
    23761 tgagattgct attttcaagg tcattccctc ctttaataga atcaatcatc attgggtttt
    23821 gtctcgtcta attgctggca gctttgaggg taccttattt tttggtgaat aataagactt
    23881 tttagcaaga tatggcattg gacgctcggc aactgtttta ctctaaaagg ttcgacggtc
    23941 acaaataaaa tcctgctact taggacaaat atctcctaag tagtggctga atcttcttcc
    24001 agaaacgacg ataactggca atcacggata attggccaaa acattagtaa aaagccagtt
    24061 tttgctcttg ctcaacatta attatgacac cattatatta caatgctgtt tcaatagaac
    24121 gcataattaa ctttctaagt attcacacat tacaaggcgc agggcgcgaa gtggcatggg
    24181 tgattatcgt ttgctatatt ttacttatca gtggcaggag tgattcgtat tcacccgcct
    24241 tgaaagcgat cgagtaggag gctacccatt aattgaatag ccgtcctctc acaccaccgt
    24301 acgtaccgtt cggtatacgg cggttcaata gaattagtgg actagtgaat atctttcact
    24361 gattgacaga agtcccagct tcttaag
//
LOCUS       DASRMS010000167         1170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_112352, whole genome shotgun sequence.
ACCESSION   DASRMS010000167 DASRMS010000000
VERSION     DASRMS010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112352"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            175..795
                     /locus_tag="VFC74_07230"
     CDS             175..795
                     /locus_tag="VFC74_07230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25165.1"
                     /translation="MKPKKLVCGVGISDAGYIVKKFEYLGHVNGRQKQKLVWACPYYQ
                     AWGNMLKRCYSTKYKERNPTYKNCTVSAEWLTFSVFKSWMEGQDWEGKQLDKDILFEG
                     NKVYSPETCVFVTKAVNMFTVDSGATRGEQLIGVSLDKRNAKFQSRCRNPFTKKMEHL
                     GLFTSEQEAHEAWLKRKLELAHELAAIQTDPRVATALVDRYSKPQE"
     gene            802..972
                     /locus_tag="VFC74_07235"
     CDS             802..972
                     /locus_tag="VFC74_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25166.1"
                     /translation="MEKEAELQELLKDKARLDWLADVNNTIGNVLLPRDIVERNLGSL
                     RDGIDEAMRIEQ"
     gene            969..>1170
                     /locus_tag="VFC74_07240"
     CDS             969..>1170
                     /locus_tag="VFC74_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25167.1"
                     /translation="MKPRITKMSVLPDNCSIFEYTATHIAIDDEGAGEYVTITQHTDD
                     RHGDHVVNINPYEWPAIRAAVNK"
BASE COUNT          368 a          237 c          282 g          283 t
ORIGIN      
        1 taatacagag ttagaattgc ggcagttttt atcagacggc attgccgaga tgctggaaag
       61 ataaacagcg ctgtggcata atatttttag cggctagggc atcgaacctg aacagtatct
      121 agtcaatact tgccgcttta aatcttcgac taccctttga ctgaggcgta tcttatgaaa
      181 cctaaaaaac ttgtctgcgg tgttggaatt agtgatgctg gttatattgt taaaaagttt
      241 gagtatcttg gccatgtgaa tggcaggcaa aagcagaagc tggtgtgggc ttgcccctat
      301 taccaagctt ggggaaacat gcttaagcgt tgttactcta ctaaatataa agagcgcaac
      361 ccaacctaca aaaactgcac cgtatctgct gagtggctta cttttagtgt gtttaaaagc
      421 tggatggaag ggcaagactg ggaaggcaag cagctggata aagatatttt atttgaaggc
      481 aataaagtct atagccctga aacatgcgtg tttgtaacaa aagcggtgaa tatgttcacc
      541 gttgatagcg gcgccaccag aggcgagcag ttgattggag taagtctgga taaaagaaat
      601 gctaagtttc agtctaggtg ccgcaaccca tttactaaaa aaatggagca cctcgggcta
      661 tttacctccg aacaagaagc ccatgaagct tggcttaaac gtaaactaga actagcccac
      721 gaactggcag caattcagac agatccacga gttgctacag cattggttga ccgttactca
      781 aaaccacagg agtaaaacta aatggaaaaa gaagccgagc tgcaagaatt gttaaaagac
      841 aaagctcgat tagactggct cgcagacgta aacaatacta tcggcaacgt gctgctaccg
      901 cgtgatattg ttgagcgcaa tctaggaagt ttgcgcgatg ggattgatga ggctatgagg
      961 attgagcaat gaaaccccgc ataactaaaa tgagcgtact gcccgacaac tgctcaattt
     1021 ttgagtatac ggcaacgcac attgcaatag acgatgaagg cgcgggcgaa tatgtcacga
     1081 taacccagca cacagatgac cgccacggtg atcacgttgt aaatataaac ccatatgaat
     1141 ggccagctat ccgcgctgct gttaataaac
//
LOCUS       DASRMS010000168         1052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_986058, whole genome shotgun sequence.
ACCESSION   DASRMS010000168 DASRMS010000000
VERSION     DASRMS010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986058"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..310
                     /locus_tag="VFC74_07245"
     CDS             <1..310
                     /locus_tag="VFC74_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HZK25168.1"
                     /translation="VSKITKTSAVSKAPAVISKRQLVGRIRDRKRHELWLESGGKCQS
                     CERAVFGKGEIHLDHIIPLSQGGTDDDDNLQILCVPCHEEKTKEELTVDGKQWSGRFY
                     "
     gene            586..>1052
                     /locus_tag="VFC74_07250"
     CDS             586..>1052
                     /locus_tag="VFC74_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25169.1"
                     /translation="MLPGQAKQKTYCGRACYGVQSSIDQGGDGNLKTKIRNCLWCNAG
                     FKRKKKINDSATYCSRGCFYEHSAHVSYELEAIRRIGVNVRSAARYKAMIKREEQILE
                     RTLKKEIDSIKKIQRLVAKEGRVRLCQRCSSKFIINSSGTRFGGKLIRCNQCA"
BASE COUNT          345 a          195 c          269 g          243 t
ORIGIN      
        1 agtcagtaag attactaaga caagcgctgt atcgaaagca cctgctgtga ttagtaaaag
       61 acagctggtc ggacgcatac gtgaccgcaa aaggcatgag ctatggttgg agtcaggtgg
      121 taagtgccaa tcgtgtgagc gtgcagtgtt cggtaagggc gagatacatc ttgaccatat
      181 catcccactt agccaaggcg gtacagacga tgacgacaac ttgcaaatac tttgcgtgcc
      241 ttgtcacgaa gagaagacca aggaagagct gaccgttgat ggcaagcagt ggagtggtag
      301 gttctattag cgtataataa acaaagcccg agcagactgg aatctggatc gggcttctac
      361 cattaacctg attagagaga atcacggcca atgaatgaat caaattgtac caacacatgc
      421 agtatttgca aggctggatt tcctttgtca ctaaaagcaa acggtgatcc taggcgtctt
      481 cagccgaagt actgtagtgc gaagtgcagt tctgtattta aaaacagacc taacaggggc
      541 atcatttata aaacatgccc tgtatgtaag tctgagtttg gacctatgtt accaggccaa
      601 gcaaaacaaa aaacatactg cggtagagct tgctacggcg ttcaaagctc tatcgatcaa
      661 ggtggcgatg ggaatcttaa aacaaagata agaaactgcc tgtggtgtaa tgcagggttc
      721 aaaagaaaga aaaagataaa cgattctgcc acttactgct cgcgtggatg tttttacgag
      781 catagtgcgc atgtcagtta cgagttggaa gcaataagaa gaatcggggt aaatgttaga
      841 agcgccgcta gatataaagc aatgattaaa agagaagagc agatcctaga aagaacactg
      901 aaaaaggaaa tcgactcaat aaagaaaatt cagcggctcg tagctaagga aggaagggtg
      961 cgtctttgcc agcgctgctc tagtaaattt ataattaata gtagcggtac tagatttggt
     1021 ggaaagctta tcagatgtaa ccagtgcgca ga
//
LOCUS       DASRMS010000169        20736 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_450743, whole genome shotgun sequence.
ACCESSION   DASRMS010000169 DASRMS010000000
VERSION     DASRMS010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 20736)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20736)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20736
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_450743"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..399)
                     /locus_tag="VFC74_07255"
     CDS             complement(<1..399)
                     /locus_tag="VFC74_07255"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HZK25170.1"
                     /translation="MKAVILNSGLGRRMEAETVDKPKCLVEIAPQETIISRQLQQLLK
                     YGLEELILTTGPFAPQLQDYLTSNFPQLQVTYVANPRYASTNYIYSLLLAKEYLNSDL
                     LLLHGDLVFDDAVLANTLASPQPNTVLVNRQ"
     gene            complement(422..1555)
                     /gene="aepY"
                     /locus_tag="VFC74_07260"
     CDS             complement(422..1555)
                     /gene="aepY"
                     /locus_tag="VFC74_07260"
                     /EC_number="4.1.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012060494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033980 - phosphonopyruvate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonopyruvate decarboxylase"
                     /protein_id="HZK25171.1"
                     /translation="MLQPAAFYRTLVEQGIANFTGVPDSLLKDFCAYVTDHATPSSNI
                     IAANEGNAVAIAAGRYLATQQISLVYMQNSGLGNIINPVTSLIDPLVYSIPLLLLVGW
                     RGQPGLKDEPQHAKQGQITLAQLEVLGIKYAILPAADTEAQAVLQEASIYMTTNQAPF
                     ALVVEKGTFAPYRLQSEQPTSFPLNREEALQTVISQLTAHDVVVSTTGKTSRELYEYR
                     QALGHDHEHDFLTVGSMGHASQIALGIALAKPQTNVYCLDGDGALLMHLGGLAIIGST
                     APTNLKHIILNNGAHDSVGGQPTVGQQIDIPAIAQACGYQTVRQAQSKKEISDQLAVL
                     QESPGPALLEIKINKGARADLGRPQSTPLENKAAFMKFLRSCR"
     gene            complement(1557..2855)
                     /gene="aepX"
                     /locus_tag="VFC74_07265"
     CDS             complement(1557..2855)
                     /gene="aepX"
                     /locus_tag="VFC74_07265"
                     /EC_number="5.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050188 - phosphoenolpyruvate mutase
                     activity [Evidence IEA];
                     GO_process: GO:0019634 - organic phosphonate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate mutase"
                     /protein_id="HZK25172.1"
                     /translation="MKKVYLAMSADIIHHGHINVIQEASKLGEVTVGILTDAVIASYK
                     RFPLLSYDERKEIIANIKGVAQVMPQETLDYVPNLEKLKPDYVVHGDDWRNGEQKVIR
                     QLVIEALQQWGGQLVEVEYTEGISITQLDEAMHKIGTTPNARMRRLRQLLEIKPTVRV
                     LEAHNGLTALIAEKTKVEQDGKIKEFDALWLSSLCDSTAKGKPDIELVDLTSRLTTIN
                     EILEVTTKPLILDGDTGGQEEHFVFTVKTLERLGVSAIIIEDKIGLKKNSLFGTEVKQ
                     TQDTIDNFAHKISAGKKAQVTESFMIIARIESLILKAGLADALARAQAYIAAGADAIM
                     IHSKEKDPAEIFAFCEAYAKFAHRVPLVAVPTSYNQVTESELADAGINVVIYANHLIR
                     SAYPAMQKAACTILEHERALECDDDLMSIKEILTLIPGGM"
     gene            complement(2893..4029)
                     /locus_tag="VFC74_07270"
     CDS             complement(2893..4029)
                     /locus_tag="VFC74_07270"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HZK25173.1"
                     /translation="MATYYNPVKIHFGVGTLQQLTTIVQTSGVQRLLLLTGARSLKAA
                     GFLDKILAQLQGIEVLLQDSVPSNPDVADIYRLHEATKHFACEAILAVGGGSVIDTAK
                     ALAAFKELQLTSVDDVRTAIQQKTYLAAAQANCPVWAVPTTAGTGSEVTPWATIWDQE
                     AGLKYSIADVTIYPQLALVDPVLTQKLSPAMTASSALDALGHALEAYWSKNTNEIVRL
                     YAREAISQIVQHLRPLLHNPTDLHLRSQVAYGSLYAGLAFSNTKTTACHSISYPLTLQ
                     YGINHGVAVSLTLGAMLVKNAEALVEKEELFTALHITSPAELNEWIQSIYAQAAIPGR
                     LRDYGISAEDLPALVEQSYTPGRMDNNPVDVDKPALLELLTSIL"
     gene            complement(4060..5589)
                     /locus_tag="VFC74_07275"
     CDS             complement(4060..5589)
                     /locus_tag="VFC74_07275"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25174.1"
                     /translation="MHKLVQQPPLVQATEVGIKYKAGRKREDFKSLFLGNSGQLGDFW
                     ALREISFSAAAADIIGIIGSNGAGKTTLCRVISRLLRPDLGTIRVNGEVSALLSLGTG
                     FNQELTGRENIFLNGMMLGFSRRHMEEISQDIVDFAGLQDFIDQPIKSYSSGMRARLG
                     FSIATMLEPEILVLDEALSTGDAEFNQRAMARTTEMVSKAKVVLIVSHDINFIAENCT
                     KAIWIDQGRLMAEGDPLEVCEQYRQMVQTSGLKQAPKKRKLRLQKTTSKTGEETVIAA
                     QDLGLCYKMPERDFWALRHCNFTVQQGEIVGIIGPNGAGKSTLCSILSGILKPDEGTL
                     ETKGDVSALLSFGSGFNKQLSGHDNIYLNALMLGMSKKKVDAVYQDIVDFSGLEKFIE
                     NPIKSYSSGMVSRLGFSIATMLEPDIFIIDEALATGDLTFYEKASARIQEMINTAKAV
                     MIVTHSMSFVQKVCTKAIWLKKGQVMHIGDPEETIALYEADTAQVMAARAAQQQQRLS
                     S"
     gene            complement(5601..6377)
                     /locus_tag="VFC74_07280"
     CDS             complement(5601..6377)
                     /locus_tag="VFC74_07280"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK25175.1"
                     /translation="MLQYLQEIKKRKDLMIYLVTSGLKAQHRNSYLGYVWWLLDPLLG
                     IGIYYFLMVILLNRGGPDYGGFLVVGMVAWRWVSSTVNASAKSIVTQSGIVSKVYLPK
                     AIFPIGTALSQLINFFFGLLIIIIYLLISRTVPGINLLWLPLVMLVQFLFLLAIGLII
                     AYLCVFIRDIDNITTHVMRLWFYASPVIWERGRLPARLSWIVKYNPISAILASYRNIL
                     LHNGAPELKKLMIIGLFSLALVAALLAFYHQNEHKIIKAL"
     gene            complement(7088..8380)
                     /locus_tag="VFC74_07285"
     CDS             complement(7088..8380)
                     /locus_tag="VFC74_07285"
                     /inference="COORDINATES: protein motif:HMM:NF016852.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LicD family protein"
                     /protein_id="HZK25176.1"
                     /translation="MASSQNLMKIMKYFTLHNLHYWLDGDSLRRLCQPQPPELADLPV
                     LNLGLTANSLPQLTVLLPELEQQGYKIKRYFYQQELFLLSLHKAGTSPLQLQIYRPLN
                     GNYYCLTFPKLTQGSLLRRLGKQLLQSLFPQLELAPFCRQKLFQLGVYLIPATYFTHF
                     ETLPHLPAVPVPHNPKEYLAQRYQFNETTKKLEDKFYQAKKPGSHLREQEYQTLTEIL
                     HRHQVPYCMDSGVLLGLMREGKLFSWEKDVDLQMWAADAPQFMAIIPELKALGWKVTL
                     WYYQGQLYQFRVSRPRRLPVHIMLFRQAGEWAWCPASRTMGNPAQRGLSWLSYAFFSK
                     LRGKLRQHLVRTDITRWPWKVRRQTATWWVPAHFFTNTAFSAVHQTYLPTSWDEYLSF
                     RYGNWRVPARDWDFWVDDGAMRHELPDAVVDFKTLAKS"
     gene            complement(8555..11110)
                     /locus_tag="VFC74_07290"
     CDS             complement(8555..11110)
                     /locus_tag="VFC74_07290"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK25177.1"
                     /translation="MFNKLAFHSKEFIKLFYSPLRSRQKSAKKQLRTKYAKYYAHYPV
                     QTDLILYESSYGRGLTCNPYAIFKAFLRDELFTKFRHVWVLENLEEHQELRESFKDYP
                     NVSFVQYESDQYLKYLVSAKYLINNTSFASYFAKKKEQIYLNTWHSITVKKLAFDLPQ
                     GRLASGNMLRNLLAADYIISANRFTTQIFKEAFKLAGLYQGKIIENGYPRNDLLLQTP
                     RTTVIKKLQALGIAVEEDKEIILYAPTWRGNNFFKPDDSITEYVEVLTTLQQQLDTSK
                     YQILIKPHPAVDKHITPEQTKQHGNLFIPGSLDTNELLAAVDILISDYSSIFFDFMLT
                     NRPILFYIPDLEQYQDQRGTYFSLAELPGPASKKLSEISSWIIHLEEMQQTYHDIYAE
                     TKAWVCEYDDGQVSEKILDIVFRRQEEPYNVLTGLPTTKKKLLIYASRLKTNGVTSSL
                     INLLQHLDYEQFDVSLIATKIKQADVIENVVKLPPQVRVFARNGTYSAALGEDIRNQL
                     VRRYGLRNWFFKKIFPQQLFQREFSRCFGNSQFDYVLDFSGYGPFFAYLLLQATGAKR
                     FIWQHSDLWVDQKRVVSGQEKTYYQRQAGIKAAFSLYPYFDKIVACGEAIMEVNRKNL
                     ATPATYAKFTCATNLVDSQRIAASLQQPPDLVMDSQGYLLRPQAVDHGRLVLGEVIKL
                     PAADTINFVTMGRLSPEKNQKNLINAFAQLHAKNANCRLYIIGEGDLRPQLEKLIAKL
                     NLQEAVFLVGSLTNPFIFLKACHCFVFPSFYEGQGLVVLEARMVGLPIIIANYGSVRD
                     VCSEKGQLVIDHDVQSIYQGLKAFLAGQVPADYRFDPESYNQKCYEEFTKLFF"
     gene            complement(11144..12676)
                     /locus_tag="VFC74_07295"
     CDS             complement(11144..12676)
                     /locus_tag="VFC74_07295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZK25178.1"
                     /translation="MDKYDTPGTKSGNPLQRLWKKFRSWKKPVQVLVVLLLVLLPAVC
                     YGAWYYYRITRPAHLFTDPPLPTPLEEEKPPEVDWDVDNYFSKNIVNIVLLGFDGSEA
                     RDEVYSVYRTDTIKVVSFNFDEEKVHIIDIPRDSYTRIATTPTYDKVNHAYYFGYKYG
                     DGDDHHASGLNYTLTSVSNILGGLPFTYYASVDMDAVVEMVDAIGGVKFDVPFDVYDT
                     KGKLRMAKGEQTLQGKNYLWYLRTRAVGGDIGRVNRQTDLLLATLDHLRNQGLIKNIP
                     TLYNTYQELIDTNLSNQQIISLAMFIGKLGRGAIQQHTLQGGGQSRDGIYYMVLDRNI
                     TSTVMQEVFGIDHLPPLQAQLTDTKPKAPKNFAAGVTGAAGKQSISLSWQAGDSHTQA
                     YHLYRSVNGRAEKLISSKQEGKSYTDQDVAPGNAYRYRLEAINHRAISDSVAVSINIE
                     ALVPPEKPEVPEVPEKPEVPVEPEEPEEPEAPEEPPTPPADPELPLDPPPADPQPPAA
                     TG"
     gene            complement(12905..13939)
                     /locus_tag="VFC74_07300"
     CDS             complement(12905..13939)
                     /locus_tag="VFC74_07300"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HZK25179.1"
                     /translation="MQTVTGNIHIHSTHSDGAGTIEEITQAAAAVGLDYLIITDHQTL
                     AGLAQEGYQQGVLVLCGSEINRTSHHYLALGIDEEVPNNDDQPQKVIDAVNEQGGLGF
                     IAHPYETGSPFVLDGITYPWENWQVEGFCGIEVWNWCSQWRDSAQGLLSTLYHAYFKP
                     EAPITGPCPQAFAQFDRLTQQQKLTAIAGSDAHDYHLRCGPLRRRIFPYQYLFRTANN
                     HLLLPQKLSSDATEAKRQILTALRQGRAFIANHLVGSAEGFSCTASSEAGIFQLGDTV
                     KLTPHAALEIICPSTPRPPSVTVIRNGQPWRQQQAHHWRLNLQLPGTYRLEVRLNSKP
                     WIFTNPLYVM"
     gene            complement(14393..15292)
                     /locus_tag="VFC74_07305"
     CDS             complement(14393..15292)
                     /locus_tag="VFC74_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HZK25180.1"
                     /translation="MAIRRKKVKGSTIVVLLLLLFFLWLASVWVRIYERSPLPEKVPQ
                     QTMPGIMNVLIVGVDQRDDEPSRADTIMIMSINQLSHEAALVSIPRDSRVPIPEHGLD
                     KINHAMQFGGLPLLRQTVENLLHIPIHYYLYTNFAGFAQMVDALGGISLEVEREIIGL
                     AGQPIVEAGPQHLTGSQALSYARFRSDAEGDFGRMRRQQQVMKAILKRLRQPASLLRL
                     PLLTEQFAQHLRTDMSIPTLAAFARTGASQDYAKLPSVQLRGRVTTIEGISYVLLDEE
                     ALQETVRRYLRWENAAITNYHAI"
     gene            15448..15714
                     /locus_tag="VFC74_07310"
     CDS             15448..15714
                     /locus_tag="VFC74_07310"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HZK25181.1"
                     /translation="MGIRIKNITEVRRRLFELMNTLHNGPLFIAKKGRVSAVLMDLAD
                     YHSLLGELEDLTGLIHTMGEGDCGCAEDDTVQRLLALDEFAVND"
     gene            complement(15877..16905)
                     /gene="mreB"
                     /locus_tag="VFC74_07315"
     CDS             complement(15877..16905)
                     /gene="mreB"
                     /locus_tag="VFC74_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004618548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HZK25182.1"
                     /translation="MSLGTDMGIDLGTATVLVHLRDKGIVLHEPSVVAIEKHSRKVLA
                     VGEAARRMLGRTPGNIIATRPLREGVIADFDVTEIMLRHFIATVSNKRRLLRPRVIVC
                     IPAATTTVEQKAVIEALQRSGARDAYLIEEPRAAALGAGLDIFQPTGHMVIDIGGGTT
                     DVAVLSLGETVVTESVRVGGNKFDEAIVRYIKKEFNLLIGERTAEELKIKVGTAFPNN
                     RDLEVEIRGRDMVSGMPRSLMASSHHVLEAINEPLEAIVRSVRHILEITPPELAGDVI
                     EHGIIMTGGGAILDGLDKLLTRETGVNVYLADDPIACVALGTGKALAQLEKITHTLIS
                     PKRISSTL"
     gene            complement(17018..17278)
                     /gene="spoIIID"
                     /locus_tag="VFC74_07320"
     CDS             complement(17018..17278)
                     /gene="spoIIID"
                     /locus_tag="VFC74_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcriptional regulator SpoIIID"
                     /protein_id="HZK25183.1"
                     /translation="MQDYIEQRVLEISNYILETGATVRQVAKKFGVSKSTVHKDVTER
                     LPFINRFIAREIKVILEKNKSERHLRGGEATRQKYLDEKKSE"
     gene            complement(17544..18239)
                     /locus_tag="VFC74_07325"
     CDS             complement(17544..18239)
                     /locus_tag="VFC74_07325"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZK25184.1"
                     /translation="MKNIFAPLAPLKKIRPSRKVLLVGGYVLVIALLLSAAVWRGYLP
                     HPRSLFAGKEPEVPPVQQPVETEPLTPPALEGEGALEGEVPAAGDETTPALTLPAVPM
                     AWPLEGTMLAEHHEVYRIGNTLRAHVGVDIRAEANTEVKAAWPGAVDRVTEDPRLGWL
                     LEIRHGGDYITQYANLLEEPYVEVGDEVKQGQAVGKVGGSAALDAVEGTHLHFAIYRE
                     GEALDPVQVISPR"
     gene            complement(18294..19304)
                     /gene="spoIID"
                     /locus_tag="VFC74_07330"
     CDS             complement(18294..19304)
                     /gene="spoIID"
                     /locus_tag="VFC74_07330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein D"
                     /protein_id="HZK25185.1"
                     /translation="MKRLVTTALLLLVLLMIGLPTLLIRACASPTEAPPEEAVDRTPL
                     SSPAEAIKLKVFQHHTSELAEMTLEEYLVGVLAAEMPASFELEALKTQAVVARTYTVN
                     QMLAYGGKGCAKHAGADICSASSHCQAWQSEEQALAKWPKNEAAGYYNKLRTAVRETA
                     GQVITHDGKLIDAVFHSTCGGQTENSEDVWSTALPYLRSVSCTYCRGSRWYKTEHAVS
                     AAKFAEAIVPYVSALPVAANGRPLLGTANRSATGRIKSLVIAGEPVTGRDFRTALGLP
                     STNVQWEVIEDKVLFTAQGYGHGVGLCQYGADGLAKAGQSCTEIINHYYKDVNITTIP
                     VP"
     gene            complement(19605..20324)
                     /locus_tag="VFC74_07335"
     CDS             complement(19605..20324)
                     /locus_tag="VFC74_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25186.1"
                     /translation="MPTKETLRFRESEETPDYIKDVVRDIADHFKTRRSYLVDFCHLI
                     SRSWNWEVVDVERNLEKWEKWFKASYKRHNPTKIIDLVKKAFFVCTTKNDIMKIRGTI
                     AEALLIGKFGGMELLNNPGGKTVGWGVVVYLNDEKVRYHCIEKKYASCRSRQTVDLGI
                     FDGYHAKFYEVKVSPSGIGCKESKYLNELTNEMEKTEITFEFWFVALDSKQAIMTRLE
                     KQKAIFEKQRFEIMGSQELSA"
     gene            complement(20329..>20736)
                     /locus_tag="VFC74_07340"
     CDS             complement(20329..>20736)
                     /locus_tag="VFC74_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25187.1"
                     /translation="IIKDSIQLVDARLIYLQCELLSGNDNFITTTFERNVELISDKDA
                     NIYLSTTLEFEKDSQFSLHCIHKGHCLATLDLNEVEFERYLYSIAVPLLLPYARECVA
                     NTMIRMGLAPYNLPTMDILLSDESNNRENEGGE"
BASE COUNT         5285 a         4881 c         5041 g         5529 t
ORIGIN      
        1 ctggcgattg acaagcactg tgttgggctg gggtgaggcc agggtattgg cgagcaccgc
       61 atcgtcaaag actaaatcgc catgtaaaag caagaggtca gagtttaaat attccttagc
      121 caagaggagt gaataaatgt agttggtcga tgcatagcgg gggttagcaa cataggtaac
      181 ctgcagctgg ggaaaattgc ttgtcagata gtcctgcagc tgcggggcaa aaggacccgt
      241 ggtgaggatt agttcctcca gaccatactt gagcagctgt tgcagctggc gggagataat
      301 ggtctcctgc ggagctattt ctaccaggca tttaggtttg tcgactgtct ctgcttccat
      361 ccgtctacca agaccggagt ttaaaattac tgctttcatt gtttccaccc gctttcgtct
      421 cttaacggca acttcttaaa aatttcataa aagctgcttt attttctaaa ggtgtagact
      481 ggggacgtcc taaatcggca cgggcccctt tattgatttt aatttctaac agcgccggac
      541 cggggctctc ttggagcaca gccagttggt cgctgatttc ttttttactt tgggcctgcc
      601 ggaccgtttg gtaaccgcag gcttgcgcta tcgccgggat gtcaatctgc tggccgactg
      661 tcggctgtcc gcccacggaa tcgtgggccc cattatttaa aatgatgtgc ttaaggttag
      721 tgggggcagt actgccaatg atggccaggc cgcctaaatg catcagcagg gcaccatcac
      781 catcgagaca atagacatta gtctgtggtt tggctaacgc gatgcctaag gcaatttgtg
      841 aggcatgccc catggagccg actgtgagaa aatcgtgctc gtggtcatga ccaagggctt
      901 ggcggtactc atagagttcg cgcgaggttt tgccggtggt ggagaccacc acatcatggg
      961 cggtaagctg ggagatgacc gtttgcaggg cctcttctct gtttaagggg aaagaggttg
     1021 gctgctcgct ttgcagacgg tagggggcaa aggtgccttt ttctacgact aaggcaaaag
     1081 gagcctggtt agttgtcatg tagatggacg cttcctggag gactgcttgg gcctccgtgt
     1141 ctgccgccgg cagaattgcg tatttaatac ccagcacctc tagttgagct agagtgattt
     1201 gaccttgttt ggcatgctgc ggttcatcct tgaggcccgg ctggccgcgc cagcccacga
     1261 gcaaaagcaa gggaatactg tagactaagg ggtcgatcag ggaagtgacg gggttgatga
     1321 tgttgccgag gccggaattt tgcatataga ccaagctgat ttgctgcgta gccagataac
     1381 ggccggcagc aatggccacg gcgttgcctt cattggcagc aataatattg ctgctgggcg
     1441 tggcatggtc ggtcacgtag gcacagaaat cttttaagag cgaatcgggg acaccggtaa
     1501 aattggcgat cccctgctcc accagggtgc gataaaaagc agctggctgc aacatcttac
     1561 atccccccag gaatgagggt gagaatttct ttaatcgaca ttaaatcgtc atcgcattcc
     1621 aaagcgcgct catgttcaag aatggtgcag gctgcctttt gcatggcagg gtaggcactg
     1681 cgaatcagat ggttagcgta aattaccaca ttaatgcctg catccgccag ctcactttcg
     1741 gtcacctgat tgtaagaggt gggcaccgcc accagaggta cacggtgggc aaatttagca
     1801 taagcctcgc agaaagcaaa gatttcggcg gggtcttttt ccttactatg aatcataatg
     1861 gcatcggcac cggcggcaat ataagcctgg gcgcgggcta aagcgtctgc cagaccggct
     1921 ttgagaatca ggctttcaat gcgggcgata atcataaagg actcggtgac ttgggctttc
     1981 ttgccggcgc tgattttatg ggcaaagttg tcgatggtgt cctgtgtttg cttaacttca
     2041 gtgccaaaga gagagttctt tttcaaacca attttatctt caataatgat ggccgagaca
     2101 cctaaccgct ctaatgtttt cacagtaaag acaaaatgct cttcctggcc gcctgtatcg
     2161 ccatcgagaa taagcggttt agtggtaact tcgaggattt cgttaatcgt ggtgagacgg
     2221 gaagttaaat caaccagttc gatatcgggt ttacctttgg ccgtggaatc gcagagactg
     2281 cttaaccaca gcgcatcaaa ctccttaatt ttgccgtctt gctccacttt cgtcttttct
     2341 gcaattaaag ccgttaggcc gttatgggct tctaaaaccc gcacggtggg cttaatttct
     2401 aacagctgcc gcaggcggcg catgcgagca ttgggcgtgg tgccaatttt atgcatggct
     2461 tcgtccagct gggtaatgga aatgccttcc gtgtattcaa cttctaccag ttgtccgccc
     2521 cattgctgca gtgcttctat caccagctgg cgaatcactt tttgttcgcc gttgcgccag
     2581 tcatcaccat ggaccacata atccggtttt agtttttcta aattcggtac atagtccagt
     2641 gtttcctggg gcatgacctg agccacgcct ttaatattgg caatgatctc tttacgctca
     2701 tcatagctta aaagcgggaa acgtttatag ctggcaatga ctgcatcagt aagaatgccc
     2761 acggttacct cccctagttt agaggcttcc tgaattacat taatatgacc gtggtgaatg
     2821 atatcggcac tcatcgctaa atatactttt ttcatagttt ttcccccatg ttactttctt
     2881 tttatcgtag ttttatagga tactggttaa cagctctagt aaggcaggct tatctacatc
     2941 tacagggtta ttgtccatcc gtcccggcgt ataagattgc tctactaaag cgggcaagtc
     3001 ctcggcagaa atcccgtaat cacgcagacg accggggata gcggcttggg cgtagatcga
     3061 ctggatccac tcatttaact cggccggaga agtgatgtgc agcgccgtaa acagctcttc
     3121 tttttccacc agcgcttctg catttttcac taacatggcc cccagagtca gactgaccgc
     3181 cacaccgtgg ttaatcccat actgtaaagt taaaggataa gaaatggagt gacaagcggt
     3241 agttttggtg ttgctgaaag ccaagccggc ataaaggctg ccgtaagcta cttggctgcg
     3301 caagtgcaag tccgtagggt tgtggagcag ggggcgcaaa tgctggacaa tctggctgat
     3361 ggcttcccgg gcataaagcc gcacaatttc gttggtattt ttagaccagt aggcttccag
     3421 ggcatgaccc agggcatcta aagctgagga cgccgtcatg gctggggaaa gcttttgcgt
     3481 caaaacgggg tccaccaggg ctagttgggg atagatggtc acgtcggcaa tggaatactt
     3541 caagccggcc tcttgatccc agatggtagc ccacggggtt acttccgagc cggtaccggc
     3601 cgtagtgggt actgcccaga cagggcagtt agcctgtgct gcagctagat aagttttttg
     3661 ctgaatggcc gtgcgcacat cgtcaacact ggtgagctgc agctctttaa aagcagccag
     3721 tgctttggct gtgtcgatca cactgccacc gcccacagcc aggatggcct cacaggcaaa
     3781 gtgcttggta gcctcgtgga ggcggtaaat atcggccaca tcaggattag agggcacact
     3841 atcctgcagc agcacctcaa tcccctgcag ctgggccaga attttgtcca ggaaaccggc
     3901 agcttttaaa gaacgggcgc cggttaagag cagcagtcgc tgcacgccgc tggtttggac
     3961 aatagttgtc agctgctgca gcgtgccgac accaaagtgg attttaacgg gattatagta
     4021 agtagccata tggtttgctt cctttcctgc tgtggtgggt tagcttgaaa gtcgttgttg
     4081 ctgctgtgcg gcccgggcgg ccatcacttg ggcggtatcc gcctcgtaga gggcaatggt
     4141 ttcctccggg tccccgatat gcatcacttg accttttttc agccagatgg ctttagtgca
     4201 gactttttgc acaaaactca tactatgggt aacgatcatt accgctttgg cggtattgat
     4261 catttcttga atacgggcgc tggctttttc gtaaaaagtt aaatcaccgg tagccagggc
     4321 ttcatcaata ataaaaatat cgggttccag catggtagca atactaaagc ctaggcgcga
     4381 gaccatgccg ctggaataac ttttaatggg gttctcgatg aatttttcca gaccggaaaa
     4441 atcaacaatg tcctgataga cagcatcgac cttttttttg gacatcccca gcatcagagc
     4501 gtttaaatag atattatcgt gaccggaaag ctgtttatta aagccggaac caaaacttaa
     4561 gagggcggag acatctcctt tagtctccag ggtcccctca tccggtttga gaataccgct
     4621 taagatactg cagagggtgc ttttgccggc gccattggga ccaataatgc ccacaatttc
     4681 accttgctgc accgtaaaat tacagtggcg cagcgcccaa aaatcccgct ccggcatttt
     4741 gtagcaaaga cccagatctt gggcggcgat gaccgtctct tcaccagttt tacttgtcgt
     4801 tttttgcagc cgcagtttgc gcttttttgg tgcctgctta agaccgcttg tttgtaccat
     4861 ctgccggtat tgctcacaca cctccagcgg gtcgccttct gccattaagc ggccttggtc
     4921 gatccagatg gctttagtac agttctcggc aataaaattg atatcgtggg agacgattaa
     4981 gacaacttta gctttgctca ccatttctgt ggtgcgcgcc atcgcccgtt ggttaaattc
     5041 agcatcaccg gtgctaaggg cttcatcgag cacgagaatt tccggctcta acatggtggc
     5101 aatactaaaa cccaaacgag cacgcatgcc ggaagaataa cttttgatcg gttggtcaat
     5161 aaagtcctgc agaccagcaa agtccacaat atcttgagag atctcttcca tgtggcggcg
     5221 ggaaaaacct agcatcatgc cgtttaagaa aatattttcc cgaccggtca gctcttgatt
     5281 aaagccggtg cccaatgaca gcagggcaga gacttcgccg ttgaccctaa tagtaccgag
     5341 gtcagggcgc aagagacggg aaatgacacg gcagagagtt gttttaccgg cgccattgga
     5401 gccgataatg ccgatgatgt cagcggcggc cgcagaaaag ctaatctccc gcagtgccca
     5461 gaaatcacct agttggccgc tgttacctaa aaaaagcgat ttaaaatctt cccgcttgcg
     5521 gcctgcttta tatttgatgc ccacttcagt ggcttggacc aaaggaggct gttggactaa
     5581 tttatgcata gctctcacct ttacagtgcc ttaatgattt tgtgttcgtt ctggtgataa
     5641 aaggccaaaa gtgctgccac caaagctaaa gagaataaac caatgatcat tagtttcttt
     5701 agttccggtg ctccattgtg cagtaagata ttacgatagc tggccaagat ggcggagata
     5761 ggattgtact taacgatcca ggaaaggcgt gccggtaagc gaccccgctc ccaaatcaca
     5821 ggtgaggcat aaaaccaaag acgcatcaca tgggtggtga tattatcaat gtcgcgaata
     5881 aagacacaaa gataagcaat gatcaggcca atggccagta aaaaaagaaa ctgcaccagc
     5941 atcaccagcg gtaaccataa aagattgatg cccggcacgg tacgcgagat cagcaaataa
     6001 ataataataa tcagcagacc gaagaaaaaa ttaattagtt gtgacaaggc tgtaccaatg
     6061 gggaagatgg ctttaggtaa ataaaccttg gagacgatac cagactgcgt cacaattgat
     6121 ttggcggaag cattaacagt ggaactaacc cagcgccaag cgaccatgcc gacaactaaa
     6181 aaaccaccat agtcggggcc gccgcggttg aggagaataa ccatcagaaa gtagtaaata
     6241 ccaatgccca agaggggatc cagcagccac caaacataac ccagataact gttgcgatgt
     6301 tgtgctttaa gaccagaggt cactaaataa atcatcaggt cttttctttt ttttatttct
     6361 tgcagatatt gcagcatagt ttttaccagc ctttttgtca agatatgttc cgtgcctgac
     6421 ctgagatgtt ttataggtcg ggcgcacaga gtgacgcccc tacgtgggtt gcatttgtaa
     6481 cgatggcttg atgcttcatg taggggcgaa ctgcgttcac ccgcacatta tttgccccag
     6541 atggtgagac acgggttttt atggacattt tataggtcgg gcgcacagag tgacgcccct
     6601 acgtagggtt gcatttgtaa cgatgggttg atgcttcatg taggggcgaa ctgcgttcgc
     6661 ccgcacatta tttgccccag aggcgaggca ctgtttttat ggacatttta tcggtcgggc
     6721 gcacagagtg acgcccctac gtgggtctgc atttgtaacg atgggttgat gcttcatgta
     6781 ggggcgaact gcgttcgccc gcacattatt tgccccagat ggtgagacac gggtttttat
     6841 ggacatttta tcggtcgggt gcacagagtg acgcccctac gtgggttgca tttgtaacga
     6901 tggtttgatg cttgtgtagg ggcgaactgc gttcgcccgc acattatttg ccccagatgg
     6961 cgaggcacgg gtttttatgg acattttatc ggtcgggcgc acaaggtgac gcccctacgt
     7021 ggggttgcat ttgtaacgat ggtttgatgc ttcatgtagg ggcgaactgc gttcgcccgc
     7081 acatttttta acttttggct aaagttttaa agtcaaccac ggcatcagga agttcgtggc
     7141 gcatcgcacc atcatctacc caaaagtccc agtcgcgggc aggaacacgc caattaccgt
     7201 agcgaaaact taaatattca tcccagctcg tgggtagata ggtttggtgg acggcagaga
     7261 aagcagtgtt tgtaaagaaa tgcgctggta cccaccaggt agctgtttgg cggcgtactt
     7321 tccagggcca gcgggtaata tcagtacgta ctaaatgctg gcgcagcttg ccacgcagct
     7381 tgctaaaaaa ggcatagctc agccaagaca ggccgcgttg ggccgggttg cccattgtgc
     7441 ggctggcggg gcaccacgcc cattcacctg cttgacgaaa aagcataata tgcacgggca
     7501 ggcggcgcgg tcgggagacg cggaattggt agagttgacc ttgatagtac caaagggtta
     7561 ctttccagcc cagtgctttt aactccggga taatggccat aaactgcggg gcatctgctg
     7621 cccacatttg taaatccaca tctttttccc aggagaaaag cttaccttcg cgcattaaac
     7681 ctagcagcac gccactatcc atacagtatg gaacctggtg gcggtgcagg atctcggtca
     7741 gagtttgata ttcttgttcg cgcaggtggc tgcccggttt tttcgcttga taaaatttat
     7801 cttccagctt ctttgttgtt tcgttgaact gatagcgctg tgccagatac tccttggggt
     7861 tgtgcggcac cggtactgct ggcaggtgcg gcaaagtctc aaagtgagtg aagtaagtgg
     7921 cgggaattaa ataaacacct agttgaaaga gtttttgtct gcaaaaaggt gctagttcaa
     7981 gttgaggaaa caagctctgt aaaagttgtt tacccagtcg cctgaggaga ctaccctgtg
     8041 tcagcttggg gaaagtaagg cagtagtaat ttccattaag cgggcggtag atttgcagct
     8101 gcagagggct ggtcccagct ttgtgtaagc tcaggagaaa aagttcctgt tgataaaagt
     8161 agcgctttat tttatagcct tgctgctcta attccggcag cagtactgtc aactgcggga
     8221 gactgtttgc cgtgaggccc aggttaagca cgggtaggtc tgccagctcc ggcggctggg
     8281 gctgacagag gcggcgcaag ctgtcaccat caagccagta gtgtaaattg tgcaaagtaa
     8341 aatatttcat aatcttcatc aggttttggg agctagccat aaagtgtgga cgtccccttt
     8401 ctgtgtatct atcgacatta tattgcaggt actgattcct gtcaatttac ctgctgccac
     8461 ctacaaactt tgctgctttt atggcaccac gctacagggc ctgcactagc tactacgtaa
     8521 tttttggtga gtttagacca gtctgtgggt ctagtcagaa gaaaagtttt gtgaattcct
     8581 cgtagcattt ttggttatag ctttccgggt caaagcgata atccgcaggg acctggccgg
     8641 ccagaaaagc cttcagccct tggtaaatac tttgcacatc gtggtcgatc actagctgac
     8701 ccttttcgct gcatacatcg cgtactgaac cataattagc aatgatgatg ggcaaaccca
     8761 ccatccgtgc ttctaaaact actaagcctt gtccttcata gaaagaaggg aagacaaaac
     8821 aatggcaggc tttgaggaaa ataaaaggat tagttaagct gcccaccaga aaaactgctt
     8881 cctgcaaatt cagcttggca atcagtttct ccagttgggg acgtaaatcc ccttcgccga
     8941 taatgtacag gcggcagtta gcattttttg catggagctg ggcgaaagca ttaatgaggt
     9001 ttttctggtt tttttccggg gataaacgac ccatggtcac aaaattgatg gtatcggctg
     9061 ccggtaactt aatgacttcc cctagcacta gcctgccgtg gtctacggcc tgagggcgca
     9121 gcagatagcc ctgactgtcc atgaccaggt ccggcggctg ctgcagactg gcggcaatac
     9181 gctggctgtc aaccagattt gtggcacaag taaacttagc gtaagtggcc ggtgtggcaa
     9241 gattcttgcg gttaacttcc atgattgctt caccgcaggc aacaatttta tcaaagtagg
     9301 gatagagaga aaatgcggct ttgatacctg cttgccgctg ataataggtt ttctcctgcc
     9361 ctgaaactac ccgcttctgg tctacccaca aatcactgtg ctgccagata aaacgtttag
     9421 cacctgtcgc ttgcagcagt aaataagcaa agaacggtcc atagccagag aaatcaagca
     9481 cataatcaaa ctggctattg ccaaaacagc gactaaattc gcgttggaaa agttgctggg
     9541 gaaagatctt tttaaaaaac caattccgca ggccataacg tctgactaac tgattacgga
     9601 tatcctcacc taaagcagca ctataggtgc cgttacgggc aaatactctg acttgcggcg
     9661 gcagtttgac cacattctca atgacatctg cttgcttaat tttagtggca atgagagaga
     9721 catcaaattg ttcgtaatcc aggtgctgca aaagattgat cagcgaagag gtcacgccat
     9781 tggtctttaa cctactagca tagatgagca gtttcttttt ggtagttggc agccccgtca
     9841 acacgttata aggctcttcc tgccgcctaa agacaatatc aagtattttt tctgagacct
     9901 gtccgtcgtc atactcacag acccaggctt tagtctcagc ataaatgtca tgatatgttt
     9961 gctgcatttc ctccagatga ataatccagc tgctgatctc gctcagcttt ttactggcgg
    10021 gaccgggcag ttcagccagc gaaaaatagg tgccgcgttg atcctgatat tgctctaaat
    10081 cgggaatata aaaaagaatg gggcgattag tgagcataaa atcaaagaag atactggagt
    10141 agtcggaaat gagaatatca acagcggcca gcagttcatt ggtatcaagg ctgccgggga
    10201 taaacagatt accgtgctgt ttagtttgct ctggcgtgat gtgtttgtcc actgccggat
    10261 gcggtttaat cagtatctga tatttactgg tatccagctg ctgttgtaat gtggtgagta
    10321 cctccacata ttctgtaata ctatcgtcag gtttaaaaaa gttattgcca cgccaagtag
    10381 gggcataaag aatgatttct ttatcctctt cgaccgcgat acctagtgct tgtagttttt
    10441 tgatcacggt ggtacgtggc gtctgcaaga gcaaatcatt gcgtggataa ccgttctcga
    10501 taattttgcc ttgatagaga ccagccaatt taaaggcctc tttaaagatt tgcgtggtaa
    10561 aacggttggc tgaaataata taatcagccg ccaagagatt gcgcagcata ttcccgctgg
    10621 ctaatctgcc ttggggcaga tcaaaagcca gtttttttac ggtaatgctg tgccaggtat
    10681 taagataaat ttgctctttt ttcttggcaa agtaactggc aaaggaagtg ttattgatca
    10741 agtatttggc agaaaccaaa tacttcagat attgatcaga ttcgtactgt acaaaactaa
    10801 cgttgggata atctttaaag ctttctctga gttcctggtg ctcctccagg ttttccagca
    10861 cccagacatg gcggaatttt gtgaagagct cgtcccgcag gaaggcttta aaaatagcat
    10921 agggattgca tgtcagccct cggccatagg aagattcgta gaggatcaga tcagtctgta
    10981 cgggatagtg agcataatat ttggcgtatt tagtgcgcag ctgcttcttg gctgatttct
    11041 ggcgactgcg caggggggaa taaaaaagct tgataaattc cttgctgtgg aaagcaagtt
    11101 tattaaacat cctcgtcacc ttctaatttt ttagacagcc aatttagcct gttgccgccg
    11161 gcggttgtgg atcggcagga ggaggatcca gtggcagttc ggggtctgct ggtggtgttg
    11221 gtggttcttc cggtgcttcc ggctcttctg gttcctccgg ttccactggc acctctggtt
    11281 tttctggtac ttctggcact tctggtttct ctggtgggac aagagcttca atgttgatgc
    11341 tgacggcgac tgaatcagaa atggcacgat ggttaatagc ttcgagacga tagcgatagg
    11401 cgttgcccgg ggcaacgtct tggtcagtgt agcttttacc ctcttgttta ctggagatca
    11461 gcttttctgc ccgaccgtta acgctgcggt aaagatgata ggcctgagtg tggctgtccc
    11521 ctgcctgcca gcttaaacta atactttgct tgccggcggc accagtcaca ccggcggcaa
    11581 aatttttggg agctttgggt ttagtatccg ttagctgcgc ctgcagcggc ggcagatggt
    11641 cgatgccaaa gacctcctgc atcacagtgg aagtaatgtt gcggtccagc accatataat
    11701 agatgccatc acgggattgt ccgccgcctt gtaaagtatg ctgctgaatg gcaccgcggc
    11761 ctagtttgcc tataaacatg gcaagtgaaa taatctgctg attggacaga ttagtatcga
    11821 ttaattcttg gtaggtgtta tataaagtgg gaatattttt gatcaaacct tgattacgta
    11881 aatggtccaa ggtggctaaa agtagatctg tttggcggtt aacacgaccg atatcgccgc
    11941 ccacagcccg ggtgcgcaga taccagagat agtttttgcc ttgcagggtt tgttctccct
    12001 tggccatgcg tagcttacct ttggtatcat aaacatcaaa gggcacatca aattttacgc
    12061 cgccgatcgc atccaccatt tccactacag catccatatc cacagaggca tagtaggtaa
    12121 aaggcagccc acctaaaata ttactgacac tggtcagggt atagttaagg ccgctggcgt
    12181 ggtggtcatc gccatcgccg tatttatagc caaaataata agcatggtta actttatcat
    12241 aagttggcgt agtagcaatg cgcgtataac tgtcgcgggg gatatcgatg atgtggactt
    12301 tttcctcatc aaaattaaaa gaaacaactt taatcgtatc agtgcggtag acagaataaa
    12361 cttcatcacg tgcttcactg ccgtcaaaac ctaacagcac aatattgacg atatttttac
    12421 taaaatagtt gtctacatcc cagtccactt ctggtggctt ttcttcttct aggggcgtag
    12481 gcagaggagg atctgtaaaa agatgcgccg gtctggtaat gcggtagtag taccatgctc
    12541 catagcagac ggcaggtagt aacaccagca gcagtaccac taatacctgt actggttttt
    12601 tccagcttct aaattttttc catagtctct gcaggggatt accagacttt gtgcctggcg
    12661 tatcatattt gtccatgctc caacctccac cactttctta attgatgata atttctacag
    12721 cgcttggcaa ttacctttaa atgttaagca aattaagtga ttatttaaca atgttaagga
    12781 agatttttag cgggctgcag cgccggctgt gctgcctttt taggctgcat tgacctgaaa
    12841 aaatatctta agctcgctga aaaaaaagaa gccgccgcgg gcttctgtct acttctgctg
    12901 taatttacat cacataaagg gggttagtaa aaatccatgg tttgctattg agacgtactt
    12961 ccaggcgata agttcccggc agctgcaggt tgagacgcca gtggtgagcc tgctgctgcc
    13021 gccagggctg accattgcgg atcacggtca cactaggcgg gcgtggcgtg gaaggacaaa
    13081 taatctcgag ggccgcgtgg ggagtgagtt tgacggtatc gcccagttga aagataccag
    13141 cttcactgct ggcagtacag gaaaagccct cagcactgcc cacaagatgg ttagcaataa
    13201 aggcacggcc ttggcgaaga gcagtaagaa tttgtcgttt ggcctctgtg gcgtcagagg
    13261 agagtttctg cggcaggagc aggtggttat tggcagtgcg aaagagatac tggtagggaa
    13321 agatgcggcg gcggagagga ccgcagcgga ggtggtaatc gtgggcgtca gagccggcga
    13381 tggccgttag tttctgctgc tgcgtgaggc ggtcaaactg ggcaaaggcc tggggacaag
    13441 ggccggtaat tggtgcctct ggcttaaagt aggcatgata aagcgtggag agtagccctt
    13501 gagcactatc acgccattgt gaacaccagt tccagacttc aatgccgcag aagccctcga
    13561 cctgccagtt ttcccagggg taagtaatac catctaaaac aaaaggggaa ccggtctcat
    13621 aagggtgggc aataaagccc agccccccct gctcgttaac agcatcaatc actttttggg
    13681 gctggtcatc gttgttaggg acttcctcgt caatgcccaa agctaaatag tggtggctcg
    13741 tacggttaat ttcactaccg caaagcacca ggacgccctg ctgatagcct tcttgcgcta
    13801 aaccagctaa agtttggtga tcagtaataa ttagataatc gagaccgacc gcggcggctg
    13861 cctgagtaat ctcctcaatg gtaccagcac catcagaatg agtgctgtga atatgtatat
    13921 taccagtcac agtttgcacg ccatcccacc tttctgttca gccagcgcca aaataacctg
    13981 ccgtagcgcg taagcttaag caacgcaaaa acccaaactc ccataaaatg tccaacacac
    14041 acgtaggggc caactgcgtt cgcccgcacc tcagccgcaa atcctcggaa attttgcgat
    14101 cacctagacc acaccaatac ctccttatct tacgcaaagt cagccagtaa ggttaatcaa
    14161 caaaaataac ctggtcataa aaatctccaa aacacacgcg taggggcgaa ctgtgttcgc
    14221 ccgcacctcg gccgcaaatc ctcgcaaatt ttgcgatcac ccagaccgca ccaacacacc
    14281 cttatcttac gcaaagtcag ccagtaaggt taatcagcag aaataacctg gtcataaaaa
    14341 tctccaaaac acacgcgtag gggcgaactg tgttcgcccg cacataatag atttaaatgg
    14401 catgatagtt ggtaatagca gcgttctccc aacgcaggta gcggcgtaca gtttcctgca
    14461 gtgcctcctc atcgagcaac acgtaggaga taccctcaat ggtagtcacc ctaccgcgca
    14521 gttggacact gggtagtttt gcatagtctt ggctggcacc agtgcgggcg aaggctgcta
    14581 aggtagggat actcatgtcg gtgcgcagat gttgggcgaa ctgttccgtt aatagcggca
    14641 gacgcagcag actggctggc tgccgcaggc gtttgaggat cgctttcatt acttgctgct
    14701 ggcggcgcat gcgaccaaaa tcaccttcag catcggagcg aaaacgggca taggataggg
    14761 cttggctgcc cgttagatgt tgtggccccg cttcaacaat gggctgccca gcaagaccaa
    14821 taatttcccg ctccacctct aaacttatac cgcctagggc atcaaccatt tgggcaaaac
    14881 cggcgaaatt ggtgtaaaga taatagtgaa tagggatgtg caataaattt tctactgtct
    14941 gccgcagcag gggcaggccg ccaaactgca tggcgtgatt aattttatct agtccgtgtt
    15001 caggaatggg gacacgagag tcacggggaa tagaaactaa agcggcctcg tgggagagct
    15061 ggttaatact cataatcata atcgtatcgg cgcggctagg ttcatcgtcg cgttgatcca
    15121 cgcctacaat cagcacattc ataatgccgg gcatagtctg ctgcggtact ttttccggga
    15181 gtggagaacg ctcataaatc cgtacccaga cagaagctag ccacaagaaa aacaagagta
    15241 acagcaggac cacaattgta ctgcccttaa ctttttttct tctgatcgcc attttgagtc
    15301 actcctcaca aattgcctct ctctaccata aactgtgcta ttaggacttt ctctattcct
    15361 gcacttttcc tgacattctt tgcctaggcg gcaggaaaag ccaacgctga gtggaattaa
    15421 aaaaatatga gaaagtggag gggaaatatg ggtattagaa taaaaaacat tactgaagtg
    15481 aggcggcgtc tgtttgaatt aatgaataca ctgcacaatg gacctctttt tatcgctaaa
    15541 aaggggcgtg tctcagcagt attgatggat ttggctgatt accatagctt gttgggggaa
    15601 ttagaagatt taaccgggct aatacatacc atgggcgaag gtgattgtgg ttgtgcagaa
    15661 gatgatactg tgcagcgctt attagctctc gatgaatttg ccgtgaacga ttagactatt
    15721 tatactttaa cagcattgct gtactatact ttttaagctt cttggtaggg cggtcttaac
    15781 cacaaagcaa agagcgaggg ggagtcctcg ctctttgctt tgttttggtg caatatcttg
    15841 tattgtttaa tgttaaagtg gcagctggca ataaagttat aaggtcgaag aaatccgttt
    15901 tggtgaaatc agggtgtggg tgattttctc cagttgtgcc agtgctttgc ccgtaccaag
    15961 ggccacgcag gcaatcgggt catccgccag ataaacattg actcctgttt cgcgggtcag
    16021 cagcttgtcc aagccgtcta aaatggcacc gccgccggtc atgataatgc catgctcgat
    16081 cacatcacca gctaattctg gtggggtgat ttctaagata tgccgcacgc tgcgcacaat
    16141 tgcctctaat ggttcattaa ttgcttctaa gacatgatgt gaactggcca tcagcgatct
    16201 tggcatcccg gaaaccatgt cgcggccgcg aatttccact tctaagtcac gattgttggg
    16261 gaaggctgtg cccaccttaa tttttagttc ttctgctgtg cgttcaccaa ttaataaatt
    16321 gaattctttt ttaatgtagc gcacaatggc ttcatcgaat ttattgccgc ccacgcgtac
    16381 actttcggtg accactgtct caccaaggga gaggaccgcg acatcagtag taccgccgcc
    16441 aatgtcaatc accatatggc cggtaggttg gaaaatatcc aggccggcgc ccagggctgc
    16501 tgcccgtggt tcttcaatta aataggcatc acgggcacca gagcgctgca gagcctcaat
    16561 gaccgctttt tgctccacgg tagtggtggc agcagggata caaacaatca cgcggggacg
    16621 caaaagcctt cttttattac ttaccgtggc gataaagtgg cgcagcataa tttccgtcac
    16681 atcaaagtcg gcaattacac cttcgcgcag tggccgcgtc gcaataatat taccgggggt
    16741 acgacctaac attctacggg cagcttcacc gaccgccaac actttgcgtg agtgtttttc
    16801 tatggctacc acggaaggtt catgtaaaac aattccttta tcacgcaaat gaactagcac
    16861 tgtagctgta cctaaatcta ttcccatatc agttccaaga ctcaaacgac ttcacctctg
    16921 cttaataaaa gcttgtccag gcaaaaaagg atcactaaag ttgtcccctc tttatctttc
    16981 tttataatat ttcgctaact tggttaattt tcctttttta ctcactcttt ttctcgtcga
    17041 ggtacttttg acgtgttgct tccccacccc gtaaatgacg ttctgacttg tttttttcga
    17101 gaatcacttt tatctcacgc gcaataaagc gattgataaa ggggagtcgt tcagttacat
    17161 ctttatgtac agtagatttg gaaacgccga atttctttgc tacctgcctg actgtggcac
    17221 cggtttccag tatataattg ctgatttcca gcacgcgctg ctcaatataa tcctgcatcg
    17281 ggccgccccc ttcaatctat cttgttaaaa aatatatgtt caggaggggc atttatgaca
    17341 cataaagcgc gattggcttt gatttccagc taatttgcct aaattttcgg ggtcaggctt
    17401 taattattca cttgcttcta tctggtgcgg gcgcacaaag tgacgcccct acgaaatata
    17461 ttttgccata gcgagaaacg tcccctgtcg caagctaccg cccatgccgg gcacaccaaa
    17521 aaaaaacagc cttgacggct gttttaacgg ggagagatta cttgtacagg atctagagcc
    17581 tcaccctcac gataaatagc aaaatgtaaa tgagtacctt caacagcatc gagggctgca
    17641 ctgccaccta ctttacctac tgcttgtcct tgctttactt catcacccac ttcaacgtag
    17701 ggttcttcca gtaaattagc gtattgggta atgtagtcgc ctccgtgacg aatctccagc
    17761 aaccaaccca ggcgaggatc ttcggtaact ctatccaccg caccaggcca agcagcctta
    17821 acttcagtgt tagcttcagc tctaatatca acgccaacat gcgcacgcaa ggtattacca
    17881 atgcgataca cttcatggtg ttcagccagc atggtgcctt ctagcggcca ggccatggga
    17941 actgccggca gcgtcagcgc aggcgtagtt tcgtcacctg cagctggaac ttccccttcc
    18001 agcgccccct caccctctag ggcaggtggc gtcagtggtt ctgtttccac aggctgttgt
    18061 acgggcggca cttctggttc tttaccagca aataagcttc gcggatgggg taagtagccg
    18121 cgccacacgg cagcagagag cagtaatgct atcaccagta catacccacc gacaagcagc
    18181 accttacggc tgggtcgtat tttctttaac ggagccaagg gtgcaaaaat attcttcatc
    18241 tggacatcac ctccttgcga ggtattatgt ccattatttt cctgcttata cgcctaaggc
    18301 acgggaattg tggtaatatt cacatcttta taataatgat taataatctc tgtgcagctc
    18361 tggcctgctt tggccaggcc atcagcgcca tactggcata agcccacacc gtgtccataa
    18421 ccttgtgctg taaaaagcac tttatcctca attacttccc actgtacatt tgtggaaggc
    18481 agcccgagcg ctgtgcgaaa atcacgcccg gtcacgggct cacccgcaat aactaaactt
    18541 tttatgcggc cggtagcaga gcggttggct gtgcctaaca gagggcggcc attggcagca
    18601 accggtaaag ctgacacata ggggacaatg gcctcagcaa atttggcagc gctcactgcg
    18661 tgttctgttt tgtaccaacg agaaccgcgg caataagtgc agctcacact acgcaggtag
    18721 gggagggcag tagaccagac atcttcacta ttttccgtct gaccaccgca ggtagaatgg
    18781 aacacggcat cgattaactt accatcatgg gtgattactt gaccggctgt ttctcgcaca
    18841 gccgtgcgca atttattata atagcccgcg gcttcatttt tcggccattt agcaagagct
    18901 tgctcttcac tttgccaagc ctggcagtga cttgatgccg agcagatatc agcccctgca
    18961 tgtttagcac aacccttgcc gccgtaagcc aacatttggt tcaccgtata ggtacgcgcc
    19021 acgacagctt gtgtctttaa agcttccagt tcaaaggatg ccggcatctc ggctgctagt
    19081 actcccacca gatactcttc cagcgtcatt tctgccagct cgcttgtgtg gtgttgaaat
    19141 actttgagct tgattgcttc agctggtgaa gatagtggcg tacgatcaac tgcttcctct
    19201 ggcggagctt ctgtgggaga agcgcaagct cggatcagca gggtgggtaa gccaatcatc
    19261 aagagaacta acagtaaaag tgctgtagtc actaatcttt tcatagtaaa agcctccttt
    19321 agcacatagt atttatatgc tgcagggggc ttgtctagta caagaaaagc gcacctattc
    19381 tactggccac atctgtaaca gcactaattg cgaaggcgat ccttaccagt agaacaaaga
    19441 tcgccaactg actataagac aatgtcagtt tacgatttta aggattccat aaattgcacc
    19501 gttaagataa tttgagtgag aataacagag acataaatat gctgatctat cagtattcaa
    19561 atagtttggt aattcacata ataggataag aaggagtgga atttctatgc ggataattct
    19621 tggctaccca tgatttcgaa tctttgtttt tcaaaaatgg ctttttgttt ttctagtctc
    19681 gtcataattg cttgctttga atcaagagca acaaaccaaa attcaaaagt tatttcggtt
    19741 ttttccatct cattagtcag ttcatttaag tatttacttt ctttgcaacc tatacctgaa
    19801 ggggaaactt taacttcata aaacttggca tggtatccgt caaaaatacc caaatcaacc
    19861 gtttgacgac ttctacaaga tgcatatttc ttttcgatac aatgataacg gactttttcg
    19921 tcgtttaaat aaactacgac accccaacct actgttttgc cccccggatt gttcaaaagt
    19981 tccatacccc cgaattttcc tattaacaat gcttctgcta ttgttcctct aatcttcata
    20041 atatcatttt ttgtcgtaca gacaaaaaat gcctttttaa ctaaatcaat tattttagta
    20101 gggttatgtc ttttataaga tgccttaaac catttctccc acttttctaa attcctttca
    20161 acgtcaacaa cttcccaatt ccaagaacgg ctaatcaaat gacaaaaatc tacaaggtaa
    20221 gatctacgag tcttaaaatg gtcagcaata tctcttacaa catcctttat gtaatcaggg
    20281 gtttcttccg attctcgaaa tcttaaggtt tctttagtcg gcaattgatt actctccccc
    20341 ctcgttctct ctattattgc tctcatcaga caaaaggata tccatagtag ggagattata
    20401 aggggctagt cccatcctta tcatcgtgtt tgcaacacat tctcttgcat aaggcaacaa
    20461 caatggaaca gcaatagagt ataaatacct ttcaaattca acctcattca aatctagtgt
    20521 ggctaaacaa tgtcccttat gaatacaatg caatgaaaat tgtgaatcct tttcaaactc
    20581 caacgtagta ctaagataaa tatttgcatc tttatcactt atcagttcaa catttctttc
    20641 aaacgttgta gtaataaaat tgtcgttgcc actcaggagt tcacactgta aatatatcaa
    20701 tctcgcatca actagttgaa tactgtcttt tataat
//
LOCUS       DASRMS010000170         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_783988, whole genome shotgun sequence.
ACCESSION   DASRMS010000170 DASRMS010000000
VERSION     DASRMS010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_783988"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="VFC74_07345"
     CDS             <1..394
                     /locus_tag="VFC74_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25188.1"
                     /translation="KMIKRTNRIALSSMHEIAFVWVRGIEGVRLFNTRTLKQVIPNRF
                     HFHAIERMQHRFNVTLLVSGNSEYVKVFSFQITEQMSHKTLVPYLNIAHDEMLGSVNP
                     NHEKHAAWVISVGEHPVDDSSLIRYLEL"
     gene            397..1293
                     /locus_tag="VFC74_07350"
     CDS             397..1293
                     /locus_tag="VFC74_07350"
                     /inference="COORDINATES: protein motif:HMM:NF012372.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HZK25189.1"
                     /translation="MNVLSLFDGMSCGQIALERAGINVSKYYAAELDKYAITVTQANY
                     PNTIQLGDVTAWREWGIDWSSIDLLIGGSPCQGFSFAGKQLAFDDPRSKLFFVYVDIL
                     NHIRSVNPDVKFMLENVKMKKEYLGVISEHLGVDPVFINSALVSAQNRQRYYWANWEF
                     GQPKDKGLVLADVIENGGVDRDKAHCIDANYYKGGNLKSYFAKSRRQLVFTEKSRALL
                     STMYKENAKSMIKRNKKGWLVLDEGLITYRKLTPIECERLQTVPSGYTAHVSNTQRYK
                     MLGNGFTVEVISHILSCNDWTV"
     gene            1324..1965
                     /locus_tag="VFC74_07355"
     CDS             1324..1965
                     /locus_tag="VFC74_07355"
                     /inference="COORDINATES: protein motif:HMM:TIGR01453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZK25190.1"
                     /translation="MFMYIYRITNLLNGKIYVGKHTCKNIENLYYGSGVAIKSAIKKY
                     GKENFKKDILCFCENEDELNSMEIEWISKLGAFGNGYNMTKGGEGQLGRKPTDAEIEK
                     ARKSRLLFYKNNPDARARLSKLAQMKVGDKNPFYRKKLTKEHIEKLTKARVAAISGEK
                     NPSATKVRCKESGVVYSTAKEAAQSVGLKHSTTILKAAKGQRKSAGCFTWELV"
BASE COUNT          676 a          375 c          529 g          522 t
ORIGIN      
        1 gaagatgatt aaacgtacca acagaatcgc gctatcaagc atgcatgaga tagcgtttgt
       61 gtgggtgcgc gggattgagg gagttaggct gtttaacacg cgtacgctaa agcaggtgat
      121 tcccaaccgt tttcacttcc atgcaataga gaggatgcag caccgattta acgtgacgct
      181 actggtcagc ggtaacagtg agtatgtaaa ggtgttctcg tttcagatca ctgagcagat
      241 gagccataaa actctagtac catacctgaa tatagcgcat gacgaaatgc ttggcagcgt
      301 gaacccaaac catgaaaaac atgcggcatg ggtaatatca gtcggtgagc atccggtaga
      361 tgatagtagt ttaattaggt atttggagtt atgaaaatga atgttttgtc cttgtttgac
      421 ggcatgtcat gcggtcagat tgcgcttgaa agagcgggta tcaatgtaag taaatactat
      481 gcagcggagc ttgataagta cgcaattact gtaactcagg ccaactaccc taacactatc
      541 cagctgggcg atgtaacagc gtggcgtgag tggggtattg attggtctag tattgacctg
      601 ctgattggcg gcagcccatg ccaggggttt agctttgcag gtaagcagct agcgtttgat
      661 gacccacgct ctaagctatt ctttgtatat gttgatattt taaatcacat tcgcagcgtt
      721 aaccctgatg tcaagtttat gcttgaaaac gtcaagatga agaaagagta tttgggcgtt
      781 atcagtgagc atttgggtgt tgatcctgtt tttatcaatt cagcattggt cagcgcacaa
      841 aacagacagc gttactactg ggcaaattgg gagtttgggc agcctaagga taaggggctt
      901 gttcttgcgg acgttattga aaatggcgga gttgatcgtg ataaagcgca ctgtatagat
      961 gctaactact ataaaggcgg aaacttaaaa agctatttcg cgaagagtcg ccgacagttg
     1021 gtttttactg aaaaatcacg agcgctttta tcaactatgt acaaagaaaa cgccaagagc
     1081 atgatcaagc gcaacaagaa aggctggctt gttcttgatg aaggcttgat aacataccgc
     1141 aaactaaccc ccattgagtg cgagcgttta caaacagtcc cgtccggata tacagcgcac
     1201 gtcagcaata ctcagcgata taaaatgtta ggtaacggtt ttacagtgga ggtaataagc
     1261 catattctgt cttgcaacga ctggacagta tagtataatg gctggacgac aagggggttg
     1321 cgtatgttta tgtatattta ccggataaca aatcttttaa acggaaaaat ctatgtaggg
     1381 aagcacacct gtaaaaatat agagaatctc tattatgggt caggggtggc aataaaatca
     1441 gcgataaaga agtacgggaa ggaaaatttt aaaaaagata ttttgtgctt ttgcgaaaac
     1501 gaggacgaat taaactcaat ggaaattgag tggatttcaa aactaggcgc atttggtaat
     1561 ggttacaata tgacaaaagg aggggaaggc caacttggta ggaaaccaac ggatgcggag
     1621 atagaaaaag caagaaaatc aagactgctg ttttacaaga acaacccaga cgcaagggca
     1681 aggttatcta aattagcaca aatgaaggtc ggagataaaa acccatttta taggaaaaaa
     1741 ttaactaagg agcatataga aaagctgacc aaggccaggg tagcagctat atctggggag
     1801 aaaaatccat cagcaaccaa agttaggtgt aaggaaagtg gggtcgttta cagtacagct
     1861 aaagaggcag ctcaatctgt cggattaaag cattccacaa caatactaaa ggccgcaaaa
     1921 ggtcagagaa aaagcgcagg atgttttaca tgggagctag tctaggcaac ggatggacgg
     1981 tcgatgtgat agcgcatata tttaatagca tttaaccagc aaaccagcct cgcacatgcg
     2041 gggctttttt aatcccgtga tatactgcaa taaaaagcga ggcgaacaca tggccagttt
     2101 ca
//
LOCUS       DASRMS010000171         1078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_861863, whole genome shotgun sequence.
ACCESSION   DASRMS010000171 DASRMS010000000
VERSION     DASRMS010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1078
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861863"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1078
                     /locus_tag="VFC74_07360"
     CDS             <1..>1078
                     /locus_tag="VFC74_07360"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HZK25191.1"
                     /translation="AIDKAQQAHKKIFLTLTVVANLSILIFFKYIVFFFEVIQDFLAV
                     FSIHMPDYEFSIILPLGISFYTFHSLSYCIDVYRGKFKATRNWRDYALYVSFFPQLVA
                     GPIVRWTFMREQIETPRKSSMQGLTVGLAFMVLGMFEKVVLSDSVFAPIANQLFALDD
                     ISSAQAWIATAAFTGQIFCDFAGYSTCAIGAALALGFRLPINFRNPYGALGFSDFWQR
                     WHISLSTWLRDYLYIPLGGNRGSALFTHRNLMLTMLIGGLWHGAAWNFIVWGGIHGLL
                     LIIERLVKQLLGGILQIKVLRPLNAIVGWLLTLLAVMFAWTWFRVTSLEQGLNWSQKL
                     LNIAELMGSARTVFIEHQLMLGCF"
BASE COUNT          227 a          239 c          262 g          350 t
ORIGIN      
        1 tcgccattga taaagcacag caggcccata aaaaaatatt tttaaccctc accgtggtgg
       61 ccaacctcag tattttaata ttttttaaat acatcgtatt tttctttgaa gtcattcagg
      121 actttctagc ggttttttca atccacatgc ctgattatga gtttagtatt attttgccct
      181 taggcatctc tttttatacc tttcattccc tgtcgtactg catcgatgtc tatcgtggca
      241 agtttaaagc aacccgtaac tggcgggatt acgccctcta tgtgtctttt ttccctcagt
      301 tggttgctgg gcccattgtg cgctggactt ttatgcgcga gcaaattgaa acaccgcgta
      361 aaagcagcat gcagggtcta acagttgggc tagcgtttat ggtgctgggc atgtttgaaa
      421 aagtggtgtt atccgacagc gtatttgccc ccattgccaa tcagcttttt gctctagatg
      481 acatcagtag tgcccaagcg tggatagcca ccgctgcttt tactgggcag attttttgtg
      541 attttgctgg ttattcaacc tgtgccatag gcgcggcctt ggcgttgggc tttcgtttac
      601 cgattaactt ccgtaacccc tatggcgcac tgggtttttc ggatttttgg caacgctggc
      661 acatatcgct ctccacatgg ttgcgcgatt acttgtatat tccgctgggc ggaaatcgag
      721 gcagcgcgct cttcacccac agaaacctca tgctaaccat gctcattggt gggttgtggc
      781 atggcgccgc atggaacttt attgtctggg gtggcattca tggtctgttg ttaattattg
      841 agcgacttgt aaagcagttg ctgggcggta ttttgcaaat aaaagtgctg cgtccgctta
      901 atgccatagt gggttggcta ctgaccttgt tagcggtcat gtttgcttgg acatggtttc
      961 gcgtgaccag tttagaacag ggtctcaact ggtcacagaa gctattaaac atagccgagc
     1021 tcatgggtag cgctcgaaca gtgtttattg agcatcagct gatgctgggt tgtttttt
//
LOCUS       DASRMS010000172         1008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_184403, whole genome shotgun sequence.
ACCESSION   DASRMS010000172 DASRMS010000000
VERSION     DASRMS010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_184403"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..216
                     /locus_tag="VFC74_07365"
     CDS             <1..216
                     /locus_tag="VFC74_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25192.1"
                     /translation="RLIAFESRIGSHLNPKSLPRTIDKYLPLDGRRKQKVSNEIRELY
                     ERRMAEYNEAKAKAKQPKVVNLKRKKE"
     gene            213..>1008
                     /locus_tag="VFC74_07370"
     CDS             213..>1008
                     /locus_tag="VFC74_07370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016032 - viral process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HZK25193.1"
                     /translation="MSLNAIITADVKNFEAGIGKATGDIDRFARVANEKLESIGKSFE
                     NAGKKASIMSAAIVAAGGVMVSSFITQEKAERKLSAAINATGGDVEVLSARFKKFASD
                     LQAVTLTGDETTLGMLQMAQTMGLTAGQSERAVKNAIAMESAFGVSAASAMRYTAALE
                     DGDATMLKRYIPALKGIEDESLLAAKAQEILGNAFEVAKEDAKTFGGSLTQLKNSAGD
                     LMEEFGAIISKALMPLVEWAKNAVEYFSNLDEGTKELIVTVGGIVAA"
BASE COUNT          326 a          180 c          275 g          227 t
ORIGIN      
        1 cgcttaattg cgtttgaaag caggattggc tcacacctaa acccgaaaag tttaccccga
       61 acgattgata agtacttgcc tttagacggc aggcgtaaac agaaggtatc gaatgagata
      121 agagagttat acgaaagaag aatggcagaa tacaacgaag ccaaagccaa agccaaacag
      181 ccaaaagtag tcaaccttaa aagaaaaaaa gaatgagttt aaacgcaatc ataacggcag
      241 atgtaaagaa ctttgaggct gggataggta aggcgactgg ggatattgac cgttttgctc
      301 gtgtggcaaa cgagaaactt gaaagcatag gcaaaagttt cgagaacgca ggcaaaaagg
      361 cttctatcat gtcggcagca atcgttgcgg ctggtggtgt gatggttagc agtttcataa
      421 ctcaagaaaa agcagagcgt aaactatccg ctgcaattaa tgccacaggc ggagacgtcg
      481 aggtactatc agcaaggttt aagaagtttg ctagtgactt gcaagcggta actctgacag
      541 gtgacgaaac cacgctgggt atgttgcaga tggctcagac gatgggtttg acggctggac
      601 aatcggagcg agcggttaag aatgcaattg ccatggagtc ggcttttggt gtttcggcag
      661 caagcgcaat gaggtacact gcagcattag aggatggcga cgcaactatg ctaaagcgtt
      721 acataccagc cttgaaaggg attgaggatg aatcgctatt agcagcgaaa gcgcaggaga
      781 tattaggtaa tgcttttgaa gtggcaaaag aggatgccaa aacttttggc ggtagtttga
      841 ctcaattaaa aaactccgct ggcgacctaa tggaggagtt tggcgcaatt atttcaaaag
      901 ccttaatgcc gttggtcgaa tgggctaaaa acgcagttga atatttcagc aatttagatg
      961 aaggcacgaa agaactaatt gtaaccgttg gtgggattgt tgctgcaa
//
LOCUS       DASRMS010000173         6510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_650220, whole genome shotgun sequence.
ACCESSION   DASRMS010000173 DASRMS010000000
VERSION     DASRMS010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 6510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6510
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650220"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     repeat_region   14..1385
                     /inference="COORDINATES: alignment:crt:1.2"
                     /inference="COORDINATES: alignment:pilercr:v1.02"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=14..41
                     /rpt_unit_seq="gtagtccccgcacatgcgggggtgatcc"
     gene            complement(1701..2234)
                     /locus_tag="VFC74_07375"
     CDS             complement(1701..2234)
                     /locus_tag="VFC74_07375"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HZK25194.1"
                     /translation="MSKKNIHLLLDDYAGEVSELTAITQYFHHHFVVAHEDVAELLEQ
                     VSLDEMRHFEMLGEALLDARIDPKVYNAHKNYWQGNNVNYQYKVCDILRADIIAELDA
                     ITQYFQHIAQIPNPATQAMLRDIIQDELQHIALFSEKLAKYCSKFDYRTWLKKNIDNY
                     QLELPDKEHVITTLRLS"
     gene            2471..3739
                     /gene="hisS"
                     /locus_tag="VFC74_07380"
     CDS             2471..3739
                     /gene="hisS"
                     /locus_tag="VFC74_07380"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HZK25195.1"
                     /translation="MLTKAPRGTRDILPTEIAAWQFFEEKVREVSRLFGYSEVRTPIF
                     EHTELFLRGVGEVTDIVSKEMYTFTDRGERSLTLRPEGTASVARAYLEHKMQGEAQPV
                     KLYYAGPMFRYDRPQAGRYRQFHQYGVEVFGSNDASVDAEVISLPLFFFRVLGLKDFT
                     LHINSVGCPKCREQYRTRLQAELKDKVGDLCKDCAERYEKNPLRILDCKNEQCQELTK
                     DVPSLLDMLCDDCAQHFAAVRQALDALGESYIVNPRLVRGLDYYTKTAFEIMSPVLGA
                     QSSICGGGRYDNLLSILGGPEVPGIGFAMGIERVLLALEAAGVQTGQDKGIDVFVATA
                     GVAAQEALKLTQDLRQAKVAADKDYLGRSLKAQLKYANKQGVRYLVILGEEEMENGQA
                     TLRDMQTGEQETVALAALVEQLAVLVREAK"
     gene            3740..5512
                     /gene="aspS"
                     /locus_tag="VFC74_07385"
     CDS             3740..5512
                     /gene="aspS"
                     /locus_tag="VFC74_07385"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HZK25196.1"
                     /translation="MEYRDRNCGEIRAAEIGQQVTLSGWVQRRRDHGKLIFLDLRDRS
                     GLVQVVFNFEHNEAIFTQAEGIRTEFVVTVQGEVIARDPEAVNPKLATGEVEVKAQSL
                     TILNKAKTPPFYIEDGVEVDENIRLKYRYLDLRRPEMLQNLILRHRVSKLVRDFLDAK
                     GFLEVETPILTKSTPEGARDYLVPSRVNPGEFYALPQSPQLFKQLLMVSGVERYFQIA
                     RCFRDEDLRADRQPEFTQIDIELSFCSQEQILELMEQMMVHIYQGALGVEIPTPFPRM
                     TYQEAMERFGSDKPDTRFALELHDISQLVAGTEFKVFKQTLAAGGVIKGINAKGCGKF
                     SRREIDELTAFVAQFGAKGLAYLFVEEAGVRSPIAKFFAEATLANIVQTLDGAPGDLL
                     LFVADEFDVACQALGALRLQLGERLGLLDENKLNFLWVVDFPLVDFDQDEGRYVAIHH
                     PFTAPRDEDLPILESEPQQVLAKAYDLVFNGVEVGGGSLRIYQRELQERMFKLLGFSA
                     DRAREQFGFLLEAFDYGTPPHGGIAFGLDRLVMLMAGEKSIREVMAFPKTASASDLMT
                     GAPSEVAPRQLKELHIQHAEKTKN"
     gene            complement(5509..5805)
                     /locus_tag="VFC74_07390"
     CDS             complement(5509..5805)
                     /locus_tag="VFC74_07390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25197.1"
                     /translation="MTFEALSTIVVLAIIVEFTTEIVKSIVPAIRGQYSRIVAILLGM
                     ILCVITRSGLLETFNIQPVVFPQIDYFITGLIISRGSNILHDLVTKLNMANVKP"
     gene            5934..6117
                     /gene="ssrS"
                     /locus_tag="VFC74_07395"
     ncRNA           5934..6117
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VFC74_07395"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            6256..>6510
                     /locus_tag="VFC74_07400"
     CDS             6256..>6510
                     /locus_tag="VFC74_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014624.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HZK25198.1"
                     /translation="MDKERQLLINRLKRIEGQVRGIGKMISEEQDCAEILMQIAAVKA
                     AVNKVGTLVFQTHFRSCLETAITEGETDFVDEVMQMLSKYI"
BASE COUNT         1643 a         1270 c         1763 g         1834 t
ORIGIN      
        1 gtattcatca tgtgtagtcc ccgcacatgc gggggtgatc ccagcagttt attttgagca
       61 tacctttcca gccggtagtc cccgcacatg cgggggtgat ccccggcgtt gatgagttcc
      121 tagttgtacc agtcagtagt ccccgcacat gcgggggtga tccccgaggt gcatgaaaag
      181 ggaaggatta taacatgtag tccccgcaca tgcgggggtg atcccataaa gccggggttt
      241 tttatgaacg acttgctgta gtccccgcac atgcgggggt gatcccttta gagccgatta
      301 tggggcgtcc gctattaggt agtccccgca catgcggggg tgatcccaca ttctatcctc
      361 tatgattttc cacaggctag tagtccccgc acatgcgggg gtgatcccaa catacacaaa
      421 ttttgatggt gtagtaatag gtagtccccg cacatgcggg ggtgatccct ggtttgtaaa
      481 aagtttgcat gctataaaga ggtagtcccc gcacatgcgg gggtgatccc gatataaaag
      541 tcactaataa gaatctagcc aggtagtccc cgcacatgcg ggggtgatcc ccattttaca
      601 tcttttgtgg tggacatatg gttgtagtcc ccgcacatgc gggggtgatc ccacgcccgt
      661 tatcggaaac tgcccgcgga atgtggtagt ccccgcacat gcgggggtga tcccttctgg
      721 atgcctcttg ctatccgctc cattgtgtag tccccgcaca tgcgggggtg atcccagtgt
      781 gaaatttgca gaactcgcat actttttgta gtccccgcac atgcgggggt gatccctagt
      841 gttgtattaa tttttcttaa atatgctagt agtccccgca catgcggggg tgatcccgta
      901 aaaggatcca ttttgagata tttgcgctcg tagtccccgc acatgcgggg gtgatccccg
      961 gtgcatagat tattgtcttg ccgacattga gtagtccccg cacatgcggg ggtgatccca
     1021 caaatttttt acggtggctc ttcgtccggt agtagtcccc gcacatgcgg gggtgatccc
     1081 aagaggatcg ccgccgcctc ctatacttgt acgtagtccc cgcacatgcg ggggtgatcc
     1141 ctgtcttccc acgtttgaac gtgtgcactt tacgtagtcc ccgcacatgc gggggtgatc
     1201 cgcactcaca acccccgaaa catggacgcc cgtagtagtc cccgcacatg cgggggtgat
     1261 ccctggatta tcttcacaaa cacaagtatt tttaagtagt ccccgcacat gcgggggtga
     1321 tcctattctg gcagaaagcc acaaataaaa tgggcggtag tccccgcaca tgcgggggtg
     1381 atcctaaaca gagcgtattt ttttgactcg cactctgttt cccacatagg tgaggtgatt
     1441 cctaaattaa gctatctgta taagtcgcgc ccttacacga gtacgcggat tataacaaat
     1501 ttgattcatg taaataaatc attacttctg gtagtgcctt tacggaacgt gggtaaaaga
     1561 taggagtgcc tcgtagtagg taggcaagta ttagaaaagc tatttaaaaa actatcctag
     1621 cgctatcttt cgatgtgcta ggatataagt cgtaattaat tgttacaatt atattaaagg
     1681 cgaattgaaa agctggtttt ttaggataac cgcagagtgg taatgacatg ttccttgtcg
     1741 gggagctcta gttggtagtt gtcgatgttt tttttgagcc aggtgcggta atcgaatttg
     1801 ctgcagtatt tggctagttt ttcggagaag agcgcgatgt gttggagttc gtcttggata
     1861 atgtcacgca gcatggcttg cgtggcgggg ttgggaattt gggcgatgtg ttggaagtat
     1921 tgggtgatgg cgtcgagttc agcaataata tctgctctaa ggatgtcgca gactttatat
     1981 tggtaattga cattgttgcc ctgccagtaa tttttatggg cattatatac tttgggatct
     2041 attctagcat cgagcagagc ttcgcctaac atttcaaaat ggcgcatttc gtcgagcgaa
     2101 acttgttcga gcagttcggc gacgtcttcg tgagcaacaa cgaagtggtg atgaaaatat
     2161 tgtgtgatgg ccgttaattc actgacttct cctgcataat cgtctagcag aagatgaatg
     2221 ttctttttgg acactataac acccccctat aattaatgat gtagttctgt atagcatatg
     2281 cagctagatc ttgttctgct agcagcagat ataacttatt gtgctattca tttgggggtg
     2341 tatagttgac aagccttggt ggattggcta gaattaaatt ggtaaattcc gactcccgac
     2401 cgcaggggcg ggggtttttt gtactagctg ggaagcagat gattttgcaa taagaagagg
     2461 gggaaatgaa gtgttaacga aagcaccgcg tggtacgcgt gatattttgc cgactgaaat
     2521 cgcagcgtgg cagttttttg aagagaaggt gcgggaagtc agtcgtttat ttggttacag
     2581 tgaagtaaga acgccgatct ttgagcatac ggagttattc ttacgtggcg tgggtgaagt
     2641 aacggatatt gtttctaaag agatgtatac ttttactgac cggggtgagc gttctttaac
     2701 gctgcggcca gaagggactg cttcggtagc cagagcttat ctggagcata aaatgcaggg
     2761 tgaggcccaa ccggttaaac tgtactatgc gggaccgatg tttcgttatg atcggccaca
     2821 ggcggggcgt taccgacagt ttcatcaata tggtgtggaa gtgtttggca gcaatgatgc
     2881 ttcagtggat gcggaagtta ttagtttacc gttatttttt ttccgggtat taggtttaaa
     2941 ggattttact ttacatatta acagtgtggg ttgtcctaaa tgccgggagc aatatcgtac
     3001 acgtctgcag gctgaattaa aagataaagt gggagacttg tgtaaagatt gtgcggagcg
     3061 ttatgagaag aatcccttac gcattttgga ttgtaaaaat gagcagtgtc aggaactaac
     3121 caaggatgta ccctcgctct tggacatgct gtgtgatgat tgtgcacaac attttgcggc
     3181 ggtgcggcaa gctttggatg ctttagggga aagctatatt gtaaacccgc gtttagtacg
     3241 gggcttggat tattacacga agacggcttt tgaaattatg tcaccagtct tgggcgcgca
     3301 gagttccatt tgtggcggcg gccgttatga taatttgtta tccattcttg gtggtccgga
     3361 ggttcccggt attggttttg cgatgggcat cgaacgagtc ctgttggcat tagaagcagc
     3421 aggggtgcag acgggccagg acaaggggat agatgtgttt gtagctactg ctggggtggc
     3481 agcccaagag gcgctcaagt tgacgcagga tttgcgccaa gccaaggtag cagcggataa
     3541 agattatttg ggacgcagcc ttaaggcaca gctgaaatat gccaataagc agggtgtgcg
     3601 ctatttagta attcttggtg aagaagaaat ggagaacgga caggctacac tgcgggacat
     3661 gcagacaggt gagcaggaaa ctgtggctct ggctgcgttg gtagaacagt tggccgtgtt
     3721 ggttagggag gcaaagtaga tggagtatcg cgatcgtaat tgtggagaaa ttcgggcagc
     3781 agagattggg cagcaggtga cgctaagtgg ttgggtgcaa agacgccgtg accacggaaa
     3841 attaatcttt ttggatttac gggatcgtag cggtctggtg caggttgttt ttaattttga
     3901 acataatgag gccatattta ctcaagcaga gggcatccgt acggaatttg tggtaacggt
     3961 gcagggagaa gtgatagcac gggatccaga ggcggtaaac cccaaattag ctactggtga
     4021 agttgaagta aaggcccagt cattaaccat tctcaataaa gcaaaaacac cgcctttcta
     4081 tattgaagac ggtgttgaag tggacgaaaa tatacgcttg aagtatcgtt atttagatct
     4141 gcggcgtccg gaaatgctgc aaaacctaat tttacgtcac cgtgtcagca aattggtacg
     4201 tgatttcctt gatgctaaag gttttctaga ggtggaaaca ccaattttaa ctaaaagtac
     4261 accggaaggc gcacgtgact atttagtacc tagtcgtgtc aatccgggcg agttttatgc
     4321 tttaccgcag tcaccccagt tatttaagca gctgttaatg gtctctggtg tggagcgtta
     4381 cttccaaatc gcacgctgtt tccgtgatga ggatttgcgt gccgaccgtc aacctgaatt
     4441 tacacaaatc gatattgaac tatctttttg tagtcaagag cagattctag agctgatgga
     4501 acaaatgatg gtgcatattt atcagggcgc tttgggggtt gagatcccta ctcccttccc
     4561 gcggatgaca tatcaggaag caatggagcg ctttggcagt gataaaccgg atacacgttt
     4621 tgctttggaa ctacatgata tttcgcagct tgtggcgggt acagagttta aagttttcaa
     4681 gcaaacattg gccgcaggtg gtgtaattaa aggcattaat gctaaaggat gcggtaaatt
     4741 cagccggcgg gaaattgatg aattaacggc ttttgttgcc caatttgggg caaaaggttt
     4801 ggcctattta tttgtcgaag aagcgggtgt gcgctcacca atagctaaat tttttgctga
     4861 ggccactctg gcaaatattg tacagactct ggacggagca cctggtgact tgttgttgtt
     4921 tgtggcagat gaatttgatg tcgcctgcca ggctttaggt gcactgcgtt tacagttagg
     4981 tgagcgttta ggcctgctgg atgaaaataa actgaatttc ctgtgggtcg tagattttcc
     5041 tctggttgat tttgatcagg atgaaggacg ttatgtcgcc attcatcatc cttttactgc
     5101 gccacgtgat gaggatttgc ctattctaga aagtgagccc caacaagtgt tagccaaagc
     5161 ctatgacttg gttttcaatg gtgtcgaagt aggtggtggc agtctgcgca tttatcagcg
     5221 tgagctgcag gaaagaatgt ttaagctgct tggttttagt gcagacaggg cacgagagca
     5281 atttgggttc ttacttgagg cctttgatta cggcacaccg cctcatggtg gcattgcttt
     5341 tggcttggat cgtttggtga tgttaatggc cggtgaaaaa tctattcgcg aggtcatggc
     5401 tttccccaaa acggccagtg ccagtgattt aatgactggt gccccttctg aagtagcacc
     5461 acgtcagctg aaagaattac atattcagca cgcagaaaaa acaaagaatt aaggtttaac
     5521 gttggccata tttaacttgg tcactaaatc atgcaagatg ttactgccgc gggaaataat
     5581 taagcctgtg atgaagtaat ctatctgcgg gaagacaact ggttgaatgt taaaagtttc
     5641 tagcaagccg ctgcgggtaa taacacagag gatcatgccg aggaggatgg caactattcg
     5701 gctatactga ccacgtattg caggaacgat tgatttgacg atttccgtgg taaattcgac
     5761 aattatggca agtacaacaa tggttgacaa ggcttcgaat gtcaaaaaaa ctcacccttt
     5821 ccccttttta ctctatattt atggtattgt ggtgcctgtt atgttgagaa cttccacttg
     5881 cttgcgtcga aaaaaattga tataattagt ttgcacataa cggagaaaaa aaagaagccc
     5941 tgcgatgtta gtgtttccgt atcatgtttt gagctaacac ttttgactcg ggagctcgtc
     6001 tctggatgca gcacatatgc cttgtatagg gaggacatgt aaagcagaca ggagtgcacc
     6061 caccctgtgg ggagagagtt caaaactaga cggttaccac ggcatagtgg ggtttttgct
     6121 ggtaagttat gtaaagcgta catttcgggt gacgaaatgt acgctttttt tagtttcaca
     6181 gttattgatc aaagttgtat ttagcaggta gtaacgatat aatttagtgg aaaattaatg
     6241 caggtggtgt tagttttgga caaagaacgt caattattga taaatcgcct caagcgtatt
     6301 gaggggcagg ttagaggaat cggtaagatg attagtgaag aacaagattg tgccgaaatt
     6361 ttaatgcaaa tagcggcggt aaaagcagct gtcaataaag taggaacttt ggtctttcaa
     6421 acacactttc gttcctgcct ggagacagcc attaccgaag gggaaactga ttttgttgat
     6481 gaggtcatgc agatgttaag taaatatatt
//
LOCUS       DASRMS010000174        46865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_318021, whole genome shotgun sequence.
ACCESSION   DASRMS010000174 DASRMS010000000
VERSION     DASRMS010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 46865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46865
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318021"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..382
                     /locus_tag="VFC74_07405"
     CDS             <1..382
                     /locus_tag="VFC74_07405"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HZK25199.1"
                     /translation="SIAEVSLEDDKIKIVADDDFKLRSVIDILKTKLIRRQVPIKNLE
                     FAKVEAASAGSVRQLVHLKQGIETDTAKKIVKDIKGLKLKVQAQILDDQVRVSGKNRD
                     DLQAVISFLKEQDYQLELQFTNYR"
     gene            504..599
                     /locus_tag="VFC74_07410"
     tRNA            504..599
                     /locus_tag="VFC74_07410"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:539..541,aa:Ser,seq:gga)
     gene            647..911
                     /gene="ffs"
                     /locus_tag="VFC74_07415"
     ncRNA           647..911
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFC74_07415"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            1065..2672
                     /gene="dnaX"
                     /locus_tag="VFC74_07420"
     CDS             1065..2672
                     /gene="dnaX"
                     /locus_tag="VFC74_07420"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HZK25200.1"
                     /translation="MTYQALYRQWRPQSFAELVGQEHVSQTLQNALVQGRLAHAYLFC
                     GPRGTGKTSVAKILAKAVNCEHGPAREPCNQCPACRGIQSGRVMDVQEIDAASNRGID
                     EIRDLRDKVRYAPVEVRCKVYIIDEVHMLTAEAFNALLKTLEEPPGQVLFVLATTEPH
                     KLPATIISRCQRLDFHLIELTEIVTRLREVAAVAGRELEEQALYRVAEEADGGLRDAL
                     SLLEQVLAYKSGIIKEEDVLAVLGAVSRDVFYELTEAVLSANLARALLLLQDVAVAGK
                     DFHHFTQQAISYYRDLMVAVACQDEASTLGVAAEWEQRLWRQASSLGLAKIGRVLAIL
                     HDLLTEIRWSPRPRLLWELAVFRIFGYDGSLPQVSAPVIEAAPVAARAPATAVKQIKT
                     QASETAATVVKENPVVKQAAPKLTQLWPRILQQVKKDNIKTYALLLSGELGSFDGAVL
                     ELHFASPFHCDMMKDEANKQALGQVLERALGTQPQIHCLLQKQGASPEATTTATATAQ
                     TENDQDDLVHSAVEIFRGKIIDDFGKK"
     gene            2687..3004
                     /locus_tag="VFC74_07425"
     CDS             2687..3004
                     /locus_tag="VFC74_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HZK25201.1"
                     /translation="MYGNMGGMMKKVQKMQKEMAQLQEELQERTLEVTSGGGMVRVEV
                     NGKKEVVNLQIDPEAVDPDDLEMLQDMIIAAVNEGLRKIDEMIMTEMQKVTGGLNLPP
                     GLL"
     gene            3020..3616
                     /gene="recR"
                     /locus_tag="VFC74_07430"
     CDS             3020..3616
                     /gene="recR"
                     /locus_tag="VFC74_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HZK25202.1"
                     /translation="MFYPDSIANLIEAMQKLPGVGPKTAQRLAFFILTLPRDEVVQMA
                     RAMVNAKDKTHYCSICGNFTDSELCVICVDKRRDSSSLCVVQDARDVLALERMREYKG
                     LYHVLGGAISPIDGIGPDQLRMRELLARLQDGRVKEVILATNPTVEGEATALYVARLV
                     KPLGIRVTRLAHGLPVGGDLEYADEVTLSRALEGRREF"
     gene            3786..4055
                     /locus_tag="VFC74_07435"
     CDS             3786..4055
                     /locus_tag="VFC74_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25203.1"
                     /translation="MEKKQKHAGPAPSTVRYFLASLKEVLQARQAAVSFFNEVTEPAE
                     VDAAIKGLTISENKYKELLQQARQAGYQIPVTLSGFEERRNLFYA"
     gene            4048..4320
                     /locus_tag="VFC74_07440"
     CDS             4048..4320
                     /locus_tag="VFC74_07440"
                     /inference="COORDINATES: protein motif:HMM:TIGR02862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pro-sigmaK processing inhibitor BofA family
                     protein"
                     /protein_id="HZK25204.1"
                     /translation="MPKIDLNLVVAAIFGLFMLYYLGKALATPARVVVRVLVTGVVGV
                     ALLFIFNLVAGLFSMKIGINAITALVVGYMGLPGLAMLVVIQTFLG"
     gene            4420..5514
                     /locus_tag="VFC74_07445"
     CDS             4420..5514
                     /locus_tag="VFC74_07445"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25205.1"
                     /translation="MREKYLAEWQPVAEKYVSPWGNDGSGHRMVEPKRAYPVSERRNF
                     ELVLNGQQPVYMPFITDMLAFSPRLIPDNFVRAWVLEINPLLPGSPCEGGPDMFGVEW
                     QYVKVTGGSMVKPGQPKIKDIRRWEEELTFPDLAAFDWEGSAQANAPLFHPERMTRVW
                     LMNGLNERLISLMDFANAMVAYVDEEQKQGVHRFFTRLCAFYDDLIEYFHHYYQADVL
                     MFNDDWGTQRGPQFSPATAREMLVPYMQRIADSCHRRGMYFELHCCGKNEQLVPAIVE
                     SNVDIWLPQENINDFELLYRLCGDKLLLGIPTDSIPAMTDAEALQSAKRFMEKYGHEG
                     HVIANGTFPVQHPKVAEYLYCLSREVYQEK"
     gene            5710..6102
                     /locus_tag="VFC74_07450"
     CDS             5710..6102
                     /locus_tag="VFC74_07450"
                     /inference="COORDINATES: protein motif:HMM:NF018685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HZK25206.1"
                     /translation="MNWKKGDLFVVAAVLVAALALFAIRALPAAAENRELRVELNGST
                     IEKITLKENDVQTLTIPLDKRGEAVVEIENGRVRMLPMSKELCPQAICSHVGWIEHAG
                     EAIVCLPNRLVLTVVGGAAEDVLDGVAR"
     gene            6102..6593
                     /locus_tag="VFC74_07455"
     CDS             6102..6593
                     /locus_tag="VFC74_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HZK25207.1"
                     /translation="MPRKVILVSACLLGLNTKYDGTHNCRPEVLAWQEQVNLIPVCPE
                     QLGGLSTPRSPTEIQDGDGHAVLKGAAEVKTAAGGNCTDAFRRGAEEVWQLAQVMAAA
                     GALLKARSPSCGSGYIYNGSFTGQTKAGDGVTAALLKINGLPVFTEEEPLQFKQWLEN
                     GDS"
     gene            6648..8105
                     /gene="guaB"
                     /locus_tag="VFC74_07460"
     CDS             6648..8105
                     /gene="guaB"
                     /locus_tag="VFC74_07460"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HZK25208.1"
                     /translation="MQPEKFGKIGITFDDVLLVPAKSDVLPSEVDVSTQLTKRIRLNL
                     PLMSAGMDTVTESRMAIAIAREGGIGVIHKNMSIDSQAAEVDKVKRSEHGVITNPFFL
                     SPQHTLGDAAALMERYRISGVPITVEGKLKGIITNRDLRFETDYSRLIDDVMTKDKLI
                     TASVGTNLQQAQEILRQHKIEKLPLVDDNFMLKGLITIKDIEKAIQYPRSAKDANGRL
                     LAAAAVGIAKDTLVRAKALVEAGVDVLVVDTAHGHSRNVLRMVESLKKEFPKVDLVAG
                     NVATGAATKDLIAAGADAVKVGVGPGSICTTRVVAGIGVPQITAILDAAEAAAAYGIP
                     IIADGGIKYSGDITKAIGAGADVVMIGSLLAGAEESPGGIEIFQGRSFKVYRGMGSLS
                     AMKEGSNDRYFQENETKFVPEGIEGRVPYRGTVGDTVYQLVGGLRSGMGYCGVANIHE
                     LKTKSQFIRITSASLRESHPHDVHITKEAPNYSLS"
     gene            8190..8387
                     /locus_tag="VFC74_07465"
     CDS             8190..8387
                     /locus_tag="VFC74_07465"
                     /inference="COORDINATES: protein motif:HMM:NF022223.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor G inhibitor Gin"
                     /protein_id="HZK25209.1"
                     /translation="MSEQKCLICSRETATGLRINTSVLCWDCEAELLQTEVTDPKYDL
                     FVKKLKQVWPPRPSLQHLAIK"
     gene            8558..9976
                     /locus_tag="VFC74_07470"
     CDS             8558..9976
                     /locus_tag="VFC74_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK25210.1"
                     /translation="MEQTRAPLWEKITEFKQKKGRVWLHVPTHGGGRGLPPLMGPIFS
                     AYAQLDLTELPGLDDLFHATGVLAEAQDLTARLWQARRSYFLVNGASAGVRAMVLATC
                     NPGDTILIARNAHGSLAQALVSVGVIPRYLPVAEVEGFPLNVTVEAVKEGLRRYPQAK
                     ALLLTSPSYFGVTANLAAIAEVVHQTQALLLVDEAHGGHFEFSDQLPTAAGQHCDLRV
                     QSWHKTLGALTPGAVLHWHRGAVDEDRLQTALQGVQTSSPPYPLLVSLDLVRQRMALE
                     GKELVGQLIEQALALRLAVGKHFPLLTPSLVQSLGFELDITRVTILTAQAGVCGLAVG
                     QQLAQQGIDLELIQPDCVLAIVGPGYREQWTARLAKSLAGMQLTSANLQLPALPPVPE
                     LVMTPREAAYRQHVTIPYQAAAERVAAAMVVCYPPGIPLCLPGERITSALITYLTGAL
                     ELGVNFRGLTKDGQLKVVKEVT"
     gene            9977..10615
                     /gene="tmk"
                     /locus_tag="VFC74_07475"
     CDS             9977..10615
                     /gene="tmk"
                     /locus_tag="VFC74_07475"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004619180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HZK25211.1"
                     /translation="MQKGYFITLEGPDGAGKTTQLARLYAAASAAGLTVVSTREPGGT
                     PVGDAIRGILLDPHYGEIIPLTEALLYAASRAQLVKEVIKPALAAGKLVLCDRFIDSS
                     LAYQSFGGGLDFDFVWRTNLAAVDGCLPDKTFILNINPQVGLARRGAAAADRMEQKSL
                     AFHQRVQQGFLQLAQQFPERLTVIEAERTAPEVFKEIWQQVAAAVTSLRGNV"
     gene            10651..11646
                     /gene="holB"
                     /locus_tag="VFC74_07480"
     CDS             10651..11646
                     /gene="holB"
                     /locus_tag="VFC74_07480"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HZK25212.1"
                     /translation="MIWNTIYGRQELVQTLQGAIEAEQIAHAYLFYGPVGIGKKTIAQ
                     VLATALNCTGAARNGCGTCESCRKAQNGTHPDLHWLRPEGKSLKIEQIRQLKKNAYLK
                     PREGNYQIFILEQADALTAEAANSLLKVLEEPPRGSLFILLAEHPSMLPATVVSRCQL
                     FVVPRLNQANIARLLQQYDLPAAEVAEIADWAEGIPGRALAMSNQTTWRTQYTAAIDT
                     LAALAAAGTVSQLAAKLEKLEELTTFLDAMTLVLREVLLWQTVGVLAKQEQQQQISQL
                     AETWSVHACIAALDEMLLLQQSLQSPVNKRLALEKMLRSLKEVRNIDANSSRNSF"
     gene            11618..12265
                     /locus_tag="VFC74_07485"
                     /pseudo
     CDS             11618..12265
                     /locus_tag="VFC74_07485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012104111.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
     gene            complement(12543..13784)
                     /locus_tag="VFC74_07490"
     CDS             complement(12543..13784)
                     /locus_tag="VFC74_07490"
                     /inference="COORDINATES: protein motif:HMM:NF019850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HZK25213.1"
                     /translation="MSPLTNNIPLLGVTGTNGKTTTILILGFILEQCGLKPTVLDSWK
                     GTVFLEQAMQQAAAQASDCLLIEVPVDALRRKQLNGRAFQCGALTNLSLDHLTSCHTP
                     DRYLDYKAGFFAQLPERAKAVINADDPSALSLAQEGHVDYITYATHYHNAMIIATNVK
                     QLQTTTNFELKVNEEFVSLNQKIISPASATVQLPLLGLHNVANALLAATLALLFVPDL
                     QAVAQALNRFPGIRRKMEVIPLAQGYLLDDAAHNPAAIQAALATIKDFAADSIYLLHG
                     IYGGGGPKLNEYNARELAHWIQQNPQCQLFITRSMYHCKSKQQVRLHEEKAILRELKE
                     KDIDFAYFPDLADAIDSLLTHLHSSNDLLVFLGGHVLDRASDLLIRAAGERPEGNLLI
                     PSELALLPRELDTQTISHNPS"
     gene            13868..14704
                     /gene="rsmI"
                     /locus_tag="VFC74_07495"
     CDS             13868..14704
                     /gene="rsmI"
                     /locus_tag="VFC74_07495"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HZK25214.1"
                     /translation="MKKGTLYLCPTPLGNLKDITLRVLDVLREASLIAAEDTRRTRKL
                     LSYYDLHTPLTSYHEHNKAAKTSQLIVKLLEGQTIAVVSDAGTPGIADPGEELVQAAI
                     AQQITVIALPGPVAAITALTASGLTAVPFAFYGFLPTHGAERKVKLAQIMGEAKTVIL
                     YESPHRLCKTLAELCAIDPKRKVVVGRELTKLHEEYLRDTLEAMSAHFDRVKPRGEFT
                     LLIAGAEPEAEEPTQDPLQLAEHYLEEGLSTREAARRAAEVTGCARNEIYRKIIEKKK
                     SE"
     gene            complement(14784..15026)
                     /locus_tag="VFC74_07500"
     CDS             complement(14784..15026)
                     /locus_tag="VFC74_07500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HZK25215.1"
                     /translation="MKSTGVVRKVDELGRVVIPIELRRTLGIDVKDSLEIYVDSERII
                     LKKYEPACLFCGNADNVKHYKGRLVCAECIKEMSAQ"
     gene            15317..17284
                     /gene="metG"
                     /locus_tag="VFC74_07505"
     CDS             15317..17284
                     /gene="metG"
                     /locus_tag="VFC74_07505"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HZK25216.1"
                     /translation="MEKKTFYITTPIYYPSDKLHIGHSYTTVAADAMARFKRLTGYNV
                     RFLTGTDEHGQKIERRAQAAGKEPQQFVDEIVAWIKNLWQTLDISYDDFIRTTEPRHQ
                     KAVQKIVQKFYDQGDIYKAKYEGWYCTPCEAFWTERQLQDGNCPDCQRPVELVAEESY
                     FFRMSKYVDRLVEHIEQHPDFIQPAARKNEMINNFIKPGLEDLCISRTTFSWGIPVPF
                     DPEHVIYVWLDALTNYITALGYMSDDDQLCQTFWPANVQLIGKEIVRFHTIYWPIFLM
                     ALGLELPEKVFGHGWLLLDDGKMSKSKGNVVDPELLVKRYGSDAIRYFLLREISFGAD
                     GHFSEEALVQRLNFDLANDLGNLLNRTLAMLKKYFAGQIPTPGASTPFDEELKNLAAE
                     LPAKLESLMDQLQFSNALAEIWKFIARANKYIDETMPWTLAKNKEEDLLRTVMYNLLE
                     SIRIVSVLLQPFMPQTPGRIWRQLGIAEETSVHTWDSILTFGQLPSNGQTVPGEVLFP
                     RLELTGEGGAPVRQATKRDKPAQEKQAPAAKEQKTVTEDGLISIDDFARLDLRVAEIT
                     AAEPVSGADKLLKLQVDVGGEVRQVVAGIAKHYSPQELLGKRILFVANLKPAKLRGVR
                     SEGMLLAASDNEGNLVLATTETPIKTGSKVK"
     gene            17284..18063
                     /locus_tag="VFC74_07510"
     CDS             17284..18063
                     /locus_tag="VFC74_07510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HZK25217.1"
                     /translation="MISLIDSHAHLTDARFQDDFTEVVARAQANGVNTILNVGCDLPS
                     SRAVVVQAEQNAGFYAAVGIHPHDARTANAKVLQEVKELAAHPKVVAVGEIGLDYYYN
                     HTPPAQQQLALRQQIRLARELALPMIIHDRDAHEDVLLALQEEKAQEVGGVMHCFSGD
                     LDFANRCLELGFYISFAGTVTFKKAGALPQVAQQIPLERLLVETDCPYLAPVPYRGQR
                     NEPANVLQVAVKVAELRQLELAQVADAASKNAQQLFSLDKF"
     gene            18125..19150
                     /locus_tag="VFC74_07515"
     CDS             18125..19150
                     /locus_tag="VFC74_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HZK25218.1"
                     /translation="MLMALPGWCTKVFGTRTVEKAILLIVLLVITSVAGIAATAAKPV
                     SLSVDGEVQALTTQAKTVGAVLAEAGLYARSCDVLTPQADKKVTHDMKISLVRAVPVY
                     LKVDKQNRLVYTTSATTAEFLTEQQVELAETDCLSPAGVSCLDNGSTVQVIRMTTDLV
                     QEETEIPFKTTQKKDPKLASGRKTVQVEGKPGMLEKTYQVVYADGVEESRELVEEKRL
                     IEPVNCVLAIGTKPVPVIASRNGTTGRVYEGMASYYADKFAGRKTAYGDAYNPEAYTA
                     AFPDKALRGKKLRVTYLKTGRSVEVLVNDFGPHTKGRLVDLSAAAARKIGLLSAGVGK
                     VKVEVLK"
     gene            19326..20006
                     /locus_tag="VFC74_07520"
     CDS             19326..20006
                     /locus_tag="VFC74_07520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HZK25219.1"
                     /translation="MKKTICILLVVILLPLVMLLTTVQLVVKNDSFFAEQYIVNNVAA
                     TTQIELAELMQITDEIQAYLLGQRRDFLIQGVVNGESRQIFNEREIQHMEDVQVLFAK
                     GLRLRNFSAIILALLLVWLWLKERVFFYQGLLASAVIFFMAAALGSFLLYMNFDRYFV
                     LFHEIFFNNDLWILDPQTSILIRMVPLNFFMSTVKQIGLLTVMQMLLLGVLGFSGRVL
                     GRRHQARI"
     gene            20395..21258
                     /gene="rsmA"
                     /locus_tag="VFC74_07525"
     CDS             20395..21258
                     /gene="rsmA"
                     /locus_tag="VFC74_07525"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HZK25220.1"
                     /translation="MSKLSNPAYVSELLAGHGIRLKKRWGQNFLVDDNILQKIAAAAD
                     LQPQDLVLEVGPGIGALTEKLGQAAGHVTTLEIDERLLPVLEETLADYKNIETIHQDA
                     LKADYQQLCAAGPLKIVANLPYNVATPLFYLWLKKFRTCIKQLVCMVQKEVAQRIVAA
                     PGGKDYGTLSVICQYAAEVQLAFEVPRTVFFPRPEVASAVLNIKPKQRTLLTPAAEVH
                     FYQIVEAVFAQRRKTILNTLHAALGMDKAEILVLGELAELDLSRRGETLAVEEFARLA
                     QMFYNKNNAAK"
     gene            21275..21751
                     /locus_tag="VFC74_07530"
     CDS             21275..21751
                     /locus_tag="VFC74_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like YkuK family protein"
                     /protein_id="HZK25221.1"
                     /translation="MDFISPSQGQLTFDETYQGITSYVDEQPHEQYRLIIGTDSQTHA
                     SQDVCFVTAIIIHRVGKGARYFYRRLNKAYMNNLRQRIYYETYLSLEVAMLVTAKLAE
                     NGDSRLNVEIHLDVGEQGATKELIREVVGMVSGSGFPAFIKPDSYGASKVADKYTK"
     gene            complement(21780..22586)
                     /locus_tag="VFC74_07535"
     CDS             complement(21780..22586)
                     /locus_tag="VFC74_07535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HZK25222.1"
                     /translation="MIVAIVPAYNEEYRLGRVLSQLLSIKLINHIFVILNGSNKLTQK
                     EAEIAQQKQPQSITLVSFSVPLGIDVPRAVGANLAYTSGADYILFVDGDMVGEYTAEV
                     TELLETATQAMLDLALFDCYPHHTELTEFQEPLFYYRHLVNQQLALAEQIRLGTPSHG
                     PHLVSRRLLNIVPWEDYAVPPTLLVHAVRHQLKVDLAGSIPHAQLGSSIKNEVHNKLV
                     TDTIIGDCLEALCMINHQPRSRYHAGKLYLGYHEQRRFDLLAQFLAGRTM"
     gene            22715..23572
                     /gene="yabG"
                     /locus_tag="VFC74_07540"
     CDS             22715..23572
                     /gene="yabG"
                     /locus_tag="VFC74_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006523177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation peptidase YabG"
                     /protein_id="HZK25223.1"
                     /translation="MAFMVGEIVGRKSYQMDLLFKIVEIDEKKGRAVLKGLDMRLLAD
                     ANLSDLSYPEQSHLQKYRKESDEAQKDCLRKIYRRRSMEREQLRSVSGRAPEEFFSLP
                     GKILHLDGDADYLGECLKAYESLEIEAVGVFAPEKEQPAQVWNLLHEHRPDILVMTGH
                     DAYIKNKKNDFQDLKYYRTSRYFVEGVLKARQYEPGKDDLVIFAGACQSHYEQLLAAG
                     ANFASSPQRVLIHALDPVFIMEKVAFTSINESVNIFEVIKSTITGLEGLGGLETRGKF
                     RLGLPKSPY"
     gene            23751..23978
                     /locus_tag="VFC74_07545"
     CDS             23751..23978
                     /locus_tag="VFC74_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HZK25224.1"
                     /translation="MQIRRNLESHVGQKIRLRAHRGRRKTLEKVGVLENTYPSIFIVR
                     VDEQNYNQRLSFSYADVLTETVELTLMQKKR"
     gene            24439..26037
                     /locus_tag="VFC74_07550"
     CDS             24439..26037
                     /locus_tag="VFC74_07550"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPOCS domain-containing protein"
                     /protein_id="HZK25225.1"
                     /translation="MGKDEFLALECITDLLKVEQVVGENASQTSVVRDIVLPGRVRKI
                     AEVDTELRDVKGRVIENKVVVEGVIHKQIFYVDSETGQMKEFTVADERFVHFVDVPGA
                     MPGMNVQVHVRAEFVGHDFIREVKAYRDAELVEDLDVEAEHEKHKKKVDSLYRQTVIL
                     EIFAKVTESMQLDVVVDVNGVDRNNVMKELLKVESVIGENASQVSVQAEIDFDRPARK
                     IKNIDTQVRNITTRVIENKVIVEGILHKQIYFVGAEDDVVHELSVDEKFTHFVDVPGA
                     FPSANVQVHPRVEFVDVDINADDPTRGKQTAIIDIFAKVTETVQVEVVTDIMGAGVRK
                     ELLKVSQVIGEGSKQHNVVANLTAPAPAKKMAGPPETRFENLETRVIEDKVVIEGDLV
                     KNVYFVRDDDEAEVREFEVREHFTVFVDIPGAMPGHVVQVYPRVEFTDFEIDPKDRTR
                     IRQTSIIEIFVKVTEHVQLEVVVSCETGPTPSVTIYVVQPGDTLFLIAQRFNVSLQAI
                     EKANPQITDPNVIFPGQQIFIPRR"
     gene            26279..27829
                     /locus_tag="VFC74_07555"
     CDS             26279..27829
                     /locus_tag="VFC74_07555"
                     /inference="COORDINATES: protein motif:HMM:NF024085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HZK25226.1"
                     /translation="MINQANKVNRIIALIQDGQEAVVRIPLAVTLPGEVEEFNEPEIT
                     VIETTATALTEEIFLEITLQQDTYYVVKETGLVNIATTVHQFTQLLPAAGSQPGHMVQ
                     LAVDPHFEEGWLAKVSKEEEKQLTILTGTCRLNAAFQLLAEEELVVEQTDTLTGKALT
                     EPIKIETVHSHFTEVVDLSLPLILTETPDSKVQARAKFINQRAAAMCGWVKVEGEVLV
                     TVSYQLAKRIIKRENYVYPLKKFFEHQTSNIEMDANVAGRTEILTCQLDPAGKGVLRG
                     LLHLSGTLIKVQKIADELRSFTSDSYAPHHYKKHFELEEVVGVGSSQTLIEQEIFFGR
                     KVKFVREPVAAEVRHLQHEIIHNKVIVRGTLHKELFAVEAKNSTVFVHDVQEKFVHFV
                     DVPGAAPGMRAQIAARVEYVRVEVNPDQETARQVAIIEIRVKVLRRIKKDHQPPVKPP
                     HDPWPKPPHYPTPPPAPEQRIYIVRSGDSVWKIAQKFGVSMEVIIAANNLQNPNLIYP
                     GQKLIIPL"
     gene            28016..28915
                     /locus_tag="VFC74_07560"
     CDS             28016..28915
                     /locus_tag="VFC74_07560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZK25227.1"
                     /translation="MRKKWTNIVLLALLLTIIIVPFAARAEAAAVRYTYRYTLTKGWQ
                     LVSAPATARITFSCKYACYRVKAGDTLESISRRFDTTVDKLLQLNRLNRAEVKSGQLI
                     LIPLCSTDPQPQPEPKPEPQPEPKPEPQPKPEPQPEPQPEPQPEPKPEPKPEPKPEPK
                     PEPQPEPQPVTGLKQAEQAMLNLLNAERTKLGLQPLQVDLELVKIARLKSQDMINLNY
                     FSHQSPTYGSPFDMMKAAGISYRTAGENLAGAPTVERAHTGLMNSPGHRANILNANYT
                     RIGIGAVSGGPYGMMFTQMFVGR"
     gene            complement(28981..29496)
                     /locus_tag="VFC74_07565"
     CDS             complement(28981..29496)
                     /locus_tag="VFC74_07565"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HZK25228.1"
                     /translation="MTRIEINRKMKKLMFYRQGQLYKTYPIAIGKPDTPTPLGEYHVY
                     EKNPQPHPGLGTRWMAFTYQRHGIHGTFNPWTINTAATAGCVRMYNEDVEEIYPLTYI
                     GTPVIIFEREEIVKIPEHIDKEIYFVRPGDTVESIAKRFHITPEQLIAFNKIKYPKYL
                     HPGKMLLIPKY"
     gene            29710..29889
                     /locus_tag="VFC74_07570"
     CDS             29710..29889
                     /locus_tag="VFC74_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HZK25229.1"
                     /translation="MSPRRERIMSDALKEELAEELGVVNVVRQEGWGGVSSRNCGNLV
                     KLAIERAERFLMNGQ"
     gene            30134..31168
                     /locus_tag="VFC74_07575"
     CDS             30134..31168
                     /locus_tag="VFC74_07575"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HZK25230.1"
                     /translation="MNELRTLEAVKQLKIAEDRVLYSANDAEIIAGATTDIYFVRTLE
                     ILRAAGKYETEVTAEIFASRAGVLCGVDEAINLLRLANVEVWSLKEGEEFAQKDVIMR
                     IKGRYSDFGVYETAILGILASSCGWATAARKIKKLAGDRPVLCFGARHVHPAVASVME
                     RAAIVGGADGCSCILGAKLAGREPSGTVPHAVMLVVGDTLEVAQLYHQTMPPEDPRIM
                     LVDTFKDEAEEALRLANSLKENLQGIRLDTPGERGGVTPGLVRELRARLDQAGFKHVK
                     LFASGGIDEERLPPLLEAGVDSFGIGSYISRASAIDMTMDIKEVEGKAIAKRGRIPGV
                     TNAPSLEKVL"
     gene            31400..32209
                     /locus_tag="VFC74_07580"
     CDS             31400..32209
                     /locus_tag="VFC74_07580"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009057 - macromolecule catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HZK25231.1"
                     /translation="MSGEQSGQLVIIGGAEDKEGECIILKRFLSLAGGRKARLVVLST
                     ASADLHAGEQYNRLFRKLGAADVRVLAIQTREEANQSQYHDGFAESTGIFFTGGDQLR
                     ITSILGGSILGSALQESYRQGAVIAGTSAGASVMSETMLVGGADDATPSKEIIRMAPG
                     LGLLKDVVVDQHFAQRGRIGRLLAMVAHNPYILGVGIDEDTAIVVNDSAEFEVTGSGT
                     VTVLDGREVTHSNVSDSSPAQPLALTNVKVHILAQDYRFNLQERFVRMPAK"
     gene            32227..34905
                     /gene="cphA"
                     /locus_tag="VFC74_07585"
     CDS             32227..34905
                     /gene="cphA"
                     /locus_tag="VFC74_07585"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HZK25232.1"
                     /translation="MKIVDLHFYTGRNIYSHYPVMRMLLDLESFQNYRTDCDPTFASR
                     ILQALPTLCEHTCSRRRPGGFIERVKEGTYLGHVVEHVFLELQHLAGIGAKYGKTYTG
                     DGRFVEIISEYRCEQAARILAEAAVQYVRSLATGVTGENGQVAASLAAAKKAASLYLP
                     GPSTAAVLAAARRRQIPYQQLQPGTSLYRLGTGKYQKRIQASLTNETGCIATDIACSK
                     PLTKKILAQHGLPVPKGRTVSSAEEAVLAAQEIGFPVAMKPDNGNQGKGVSLNLNSMA
                     EVQKAFSRAAVFSPSVMVEACLDGRHYRLLVVKGQLAAAAERLPAFVTGDGIHTISEL
                     VSEENKNPLRGDGHEKPLTKISLDGVAEEVLLRQGMVAQTVVPASVRVYLRDNANLST
                     GGTSCDVTDEVHPQQIALAVQAASYIGLDVAGVDLVMKSIALPPAEQAGGIIEVNAAP
                     GLRMHLYPSVGKKRDVGEKIVSALFPFEQPTRVPVISITGTNGKTTTTRMLEYVMRQQ
                     GLFTGMCCTDGLYYNGQLARSGDLTGPDGAGSVLAHPEVEVAVLETARGGIVRRGLGY
                     ERADVAVICNVREDHLGQDGIESLEDLVHVKSLVAEAVYENGTVILNADEANVTEIAA
                     RVWERIIYFSRQEDNIIVRRHLGKGGRALFIRRGAIVAAQGNRVVLVGRVRDFAVTFA
                     GRAQHQVENLLAALAACWGTGLSPRQAGFYLRNFTSSPQDNPGRANLYQVGSVQVLVD
                     YGHNPDGFAKIGDLVRKINTKRTLAVIGVPGDRCNELIFTAGKTAAQFFDHLLIKEDA
                     DLRGRRPGEVTALLREGALAAGIKLNCITEIAQETKAVRTALQMAAAGDLVVVFYEEL
                     EPVIAEIIASQQQQRSLLTAPKEKKELSYDSPAMLT"
     gene            34943..35839
                     /gene="ispE"
                     /locus_tag="VFC74_07590"
     CDS             34943..35839
                     /gene="ispE"
                     /locus_tag="VFC74_07590"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF011202.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HZK25233.1"
                     /translation="MNSSEQQTLVIEQAYAKINLILAVTGKRIDGYHDLQTVFQSLAL
                     HDTLTFTANESEVLTLTTNNPSLPAGPDNLIWRAAERLMEFYKVKKGVAINLEKRIPL
                     AAGLGGGSSDAAATLRGLVRFWQLPCEPQVLSEIAAELGSDVPYCLAGGTALGTGRGE
                     QLLQLTPCPHFYVVLANPGFPVSTAQVYQDLRTTELASSAALHGMLRALAEQNQTAMM
                     HHVANTLEKATFRLYPQVKALKTEMASASAALMCGSGPTVFALFLTSAAATAVQAALQ
                     QKGYQVWLTETIQQPEVEKWSM"
     gene            35827..36588
                     /locus_tag="VFC74_07595"
     CDS             35827..36588
                     /locus_tag="VFC74_07595"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HZK25234.1"
                     /translation="MVDVVILAGTAKQSELTISEKVQNKAFVQIHGQAMLSYVLDALQ
                     QASSVNRIAIVGPLAELQTLIGASPQLFAVAEGDTIVENLQLGFAALQPKQHCLVVTA
                     DVAFLTAAAVEDFLAACQPYESAVYYPIVAREDNDRRFPGVKRTYVKIHDGEFTGGNL
                     FLVDPACLTKVLPRLEHFFALRKSPLRLAGALGLGFVVKLLTKRLTIAELEKRFYALF
                     AVAGKAVISQYAEIGTDVDKPADLELARQHLKTQL"
     gene            36795..37070
                     /gene="spoVG"
                     /locus_tag="VFC74_07600"
     CDS             36795..37070
                     /gene="spoVG"
                     /locus_tag="VFC74_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016206862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HZK25235.1"
                     /translation="MRVTDVRIRKLNHDGKMKAIVSITLDNQFVVHDVRVIEGNNGLF
                     VAMPSKRTPDGEFKDIAHPINSETREIIQSAVLLAYNTELERELVGA"
     gene            37290..38663
                     /gene="glmU"
                     /locus_tag="VFC74_07605"
     CDS             37290..38663
                     /gene="glmU"
                     /locus_tag="VFC74_07605"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HZK25236.1"
                     /translation="MANRAAVVLAAGEGKRMKSASPKVLHSVCGKPMLEHVVSAVSSV
                     CSEIIVVIGHQGHEVKQAFGERVSYVEQAQQLGTGHAVLQAVPLLPTSGTVFVLCGDT
                     PLLSAETMQNLLEAHQKAQAGATILTACLPEPFGYGRIIRDNGRVSKIVEERDATDEE
                     RKVCEINTGTYVFDAQLLREALANLHTNNAQGEYYLTDCIEQIISKAHLVTTCILEDH
                     RLALGVNDRLQLAQAQSLMQERINRTLMAAGVTMIDPQSTYVDAGVSIASDTVIMPNT
                     VLRGATQIGHHCVIGPNSELIDCQVGNEVVFRHSVATSCRLADKVTVGPFAHLRPETV
                     LENGVKVGDFVEIKKSQVGENTKIAHLSYIGDAQIGSDVNMGGGIIVVNYDGRKKHQT
                     VIGTRAFIGCNSNLVAPVTIGEGAFVAAGSTITKNVPAGALSLARAQQVNKEGRATRL
                     LKKNNDE"
     gene            38740..39681
                     /locus_tag="VFC74_07610"
     CDS             38740..39681
                     /locus_tag="VFC74_07610"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HZK25237.1"
                     /translation="MYRGKKIHIFSGSANLQLTEEIAQHIDLPLGQAEVKRFSDGEVN
                     ICIGESVRGSNVFIVQPICCPANENLMELLIMMDALKRASAKSINAVLPYYGYARQDR
                     KARARDPITAKLVADLLTTAGANRIITMDLHAGQIQGFFNIPVDHLKSLPILANYFLQ
                     KEFTDGVVVSPDVGGVTRARALAERLQLPIAIIDKRRPEANVAEIMSIIGNVKGKNVI
                     MLDDIIDTAGTITLGAQALIEKGAKDVYACCTHPVLSGPALQRLADSPIKEIVVTNTI
                     PQRTEDNGKIKVLSVAPLIGDAIIRIHEELSVSKLFD"
     gene            39749..40390
                     /locus_tag="VFC74_07615"
     CDS             39749..40390
                     /locus_tag="VFC74_07615"
                     /inference="COORDINATES: protein motif:HMM:NF004133.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HZK25238.1"
                     /translation="MERMQVVAQPRAAATKGQLNALRKAGSVPGVVYGRKSEPISVAV
                     DSKDIMAILQSPSGANTLVDLSVDGNKATVMIKDLERDFLVADRYTHVDFLRISLLDK
                     LEVQVPIALIGDAPGVKDGGILQHSLREVTLKCLPTNIPDSLELDISGLALGDSLTVA
                     DLAVPADSDLLSEADEVIVSIVAQRVEEEAETTDDAEVEDAAAETADSEAGQE"
     gene            40400..40963
                     /gene="pth"
                     /locus_tag="VFC74_07620"
     CDS             40400..40963
                     /gene="pth"
                     /locus_tag="VFC74_07620"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HZK25239.1"
                     /translation="MFIILGLGNPGPQYARTRHNVGFRVLDQLSAATKIPLYKVGYHA
                     FWGKGTMAGQTVILAKPMTYMNNSGLAAAALARQFKVTADKILTIYDDLDLPLGALRV
                     RAQGSSGGHNGIKSLIFHLQTEHIPRLKVGIDRPSEQNVIDYVLQSFSAAEEEALAPV
                     LKDAVSAAQLFVREGIQATMNSFNSSP"
     gene            41156..41386
                     /locus_tag="VFC74_07625"
     CDS             41156..41386
                     /locus_tag="VFC74_07625"
                     /inference="COORDINATES: protein motif:HMM:NF022408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010468 - regulation of gene expression
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-F factor Fin"
                     /protein_id="HZK25240.1"
                     /translation="MLITWICRNCERQVMRLNVSEFDSRVAALTAQAGDDIIERDQEG
                     NLVIHLLCEHCLEVAYREDESEINFIRGPELH"
     gene            41492..45007
                     /gene="mfd"
                     /locus_tag="VFC74_07630"
     CDS             41492..45007
                     /gene="mfd"
                     /locus_tag="VFC74_07630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HZK25241.1"
                     /translation="MKNIAAAIAQMSQLQPLFEGLHKTTRSQLLYGLDESARHMVMAA
                     IYEQTRRSVIIVTADAVHANKVYEDLLAVFGENKVFLFPGKELLYYHDLLSDSGDTAA
                     QRLHVMKELAADKQPIVVTTVSGLVEKMCPREQWQAACFTVKVNAELGMEQLLKLLVT
                     AGYERVELVEVAGQFSVRGGIADIFPIGEATPYRIEFFGDLIESIRSFDPLTQRSQES
                     LQSLQLAPVREVIVGEEQQQQALTALAAEQQKLAAQKRQEIRGGGQLAEKLAQEMEKI
                     KEGLYYPSLERLLLYFYPQPATLLDWFPAEALIFLNDPQRCEQAVEQLKHELGEIQST
                     LFAQGELLAHQVEMTWDYEEVLSLTKQQIVAFALFTQQGFPVPYRRTISFSAKPVPRF
                     LGQWELFCQEVEQWRQLAYRVVIITSTRQRSSGITDILAERNIPSVYSLSQPDLAPRT
                     VTLLHGSLESGFILPELKLAVLAEQDILPQRKKRRRLKGKEGVRVGDYQELQVGDFVV
                     HEQHGIGQYLGLRTLEVSGIQRDYLYIQYAGNDKLYLPIEQIDVVRKYLGAEGKRPKL
                     SALGGGEWSRVKARVQSSVQELAKELLALYAAREAEPGHAFAADHDWQQDFEAAFPFE
                     ETEDQLQAIKEIKEDMEKSRAMDRLLCGDVGYGKTEVAMRAAFKAIMDGMQAAFLVPT
                     TVLAQQHYRTFLERLEGFPVKVGILSRFQSTAQQKVTLQGLEEGSIDLLVGTHRMLSQ
                     DVRFNKLGLLVVDEEQRFGVRHKERIKMLKKNLDVLTMTATPIPRTLHMSLAGVRDMS
                     VIETPPEDRYPIQTYVLEYSDSLIREAILRELGRGGQVYFVHNRVQSIERWAAQLRKL
                     LPEAKLAVGHGQMPEGRLEKIMLDFLDGEYDVLLSTTIVEAGLDIPNVNTIIIHDADK
                     FGLAQLYQLRGRVGRSNRIAYAYLTYQKDKVLTEVAEKRLQAIKEFTELGSGFKIALR
                     DLEIRGSGNILGPEQHGHMMAVGFDMYMKLLEDAINAYKGTKKEQKMLPRIEVEADAY
                     LPASYISDTRQKIVFYQKVAAVETAEEVAEAREELQDRYGSLPKAADNLLQVALIRLL
                     ASDLEIMTITEERREIMLRFATKTPLASRTLQIVAKRHQGRLTMAMGKQFVLAFRKQF
                     KEDREKLIFLSQLLNELKILLAKQA"
     gene            45049..45939
                     /locus_tag="VFC74_07635"
     CDS             45049..45939
                     /locus_tag="VFC74_07635"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HZK25242.1"
                     /translation="MSQKHNMKFTLGLLLTLLLLLSGCRGRQVVAEVNGDTIKRAELD
                     DYKNVLSFLMPDLAPMLNEAKDRQALEEIILQTLIENKLLVQLAAEREIAITDEELTA
                     AVEQQAETISTMSGVDDLTQPREELNITDDQLKAFIAGTLYIEKLIAKITPELEPKEI
                     DDFQSEQPTAGVMLALSQILVQTESEAEIVRTRLLAGEELAIVAQFLPAPTALIKQGE
                     LGCVPAATTAYPESFMTAANQLAAGEISAPVQTGLGWHIIVRHERQVPTKEEAAAFLA
                     RERLVTIFDEYYQRAEIKLR"
     gene            46148..46702
                     /gene="spoVT"
                     /locus_tag="VFC74_07640"
     CDS             46148..46702
                     /gene="spoVT"
                     /locus_tag="VFC74_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein T"
                     /protein_id="HZK25243.1"
                     /translation="MKATGIVRRIDDLGRVVIPKEIRRTLKIREGDPLEIFVDHGGEV
                     ILKKYSPIGELGDFAQEYADSLYEAMGHIALIADRDTIVAVAGAPKKEFMNRPLSPAV
                     EKVMEDRKSVLIGNTAEHKFYKELDKDDAPKFMVEAIAPIISGGDPIGAVILGSKDQE
                     VKMSDLEMKLVETAAGFLAKQMEQ"
BASE COUNT        13033 a         9475 c        11905 g        12452 t
ORIGIN      
        1 tagtatagca gaagttagtt tagaagatga taagataaaa attgtggcag atgatgactt
       61 taagctgcgc agcgtcattg atattttaaa aacaaaattg attcgccggc aagtgccaat
      121 taaaaattta gaatttgcta aagtagaagc agccagtgct ggcagcgtgc gtcagctggt
      181 acatttaaag caaggaattg aaacggatac tgcaaaaaaa atagtcaagg acatcaaagg
      241 cttaaagctt aaagtacaag cgcaaattct agatgatcaa gttcgtgttt ctgggaaaaa
      301 tcgtgatgat ttacaggcgg tcatcagctt tttaaaagaa caagactatc aattggagtt
      361 acagtttaca aactaccgtt aacggctgtc tcgtaaattg gtaacaataa gtacttgaaa
      421 taaagcttaa ggagtagtag aataagatat tgctggggta gtttatgccc tggtggaaga
      481 taatagtgaa gtagctgtag tccggagaga tggctgagtt ggacgaaggc gctcgcctgg
      541 aaagcgagta gacggggcaa aacctcgtct cgagggttcg aatccctctc tctccgccac
      601 ctatctattg atagtaggat aaataaatac tgaaaaacaa gtttggtcgt gctagacggg
      661 gaggtagcgg tgccctgtac ctgcaacccg ctatagcagg gtcgaattcc cgccctaggc
      721 tccagcttct tagggtctga cccaggtaag tggcgttgaa gatcgggtcc cgtgcggcgt
      781 aagcttgtaa accccgtcag atccggaagg aagcagcggt aagcaagtac ttgcgggtgc
      841 cacggggcag cctggtccga gctaactgct tgggtaacgc ttgagagaaa gagacaacgg
      901 taggtgcacg accattaata ttgtatcatc atcataacaa ttaattcctc tcaacagagg
      961 aatttttttg tataccttac tggcacagac aagttaagac tggcaggatt ttatctttgt
     1021 atggggaatt aggctataga ttacaaaaaa attgggtggt atgaatgact tatcaagcgc
     1081 tttaccgcca gtggcgtcca caatcatttg cagaactagt aggacaggag catgtcagcc
     1141 aaactttgca gaatgcgtta gtgcaaggac ggctggcaca tgcttatttg ttctgcggcc
     1201 cacggggaac gggcaaaacc agtgtcgcca aaatactggc aaaggccgta aattgtgagc
     1261 atggtccggc tcgggagcct tgtaaccaat gccctgcttg tcgtggtata cagtctgggc
     1321 gcgtgatgga tgtacaagag attgatgcag cctctaaccg gggtatagat gagattcgcg
     1381 acctgcgtga taaggtgcgc tatgcaccag tagaagtgcg ctgcaaggtg tatattattg
     1441 acgaagtgca catgttaact gcggaggcgt ttaatgcctt gttaaaaacg ctggaagagc
     1501 ctccggggca ggttttattt gtcttggcaa ccactgagcc gcataaatta ccagctacca
     1561 ttatctctcg ctgtcaacgc ctggattttc atttaattga actgacagaa atcgttactc
     1621 gtttgcgtga agtagcagca gtggcggggc gggaacttga ggagcaagcc ttatatcgtg
     1681 tggcagaaga agcagatggt gggttaaggg atgctttatc acttttagaa caagtactgg
     1741 catacaaatc tggcataatc aaagaagaag atgtgctggc agtgctggga gccgtaagta
     1801 gagatgtatt ttatgaatta acagaggccg tgttgagtgc taatttggcg cgggctttac
     1861 tactattgca ggatgttgcc gtagcgggaa aagattttca tcattttaca caacaagcca
     1921 tatcatatta ccgtgactta atggtagccg tggcttgtca agatgaggcc tctactttag
     1981 gtgtggccgc agaatgggag caaaggttgt ggcgtcaggc cagctcgctg ggtttggcta
     2041 aaatcggccg cgttttagct attctgcatg atctcctcac agaaatacgg tggtccccca
     2101 gacctcgttt attatgggag ttagctgttt ttcggatctt cggctatgat ggttcgctac
     2161 cacaagtaag tgcgccagtg atagaggctg cccccgtggc agcaagagca cctgccactg
     2221 cagtaaagca aattaaaacc caagcaagcg aaacagcagc gacagtggtc aaggagaacc
     2281 ccgtggttaa gcaggccgca ccaaagctga cacagctttg gccgcgcatt ctgcagcagg
     2341 tcaaaaagga taacattaaa acctatgccc tattactttc cggagaatta ggttcctttg
     2401 atggtgcagt gctagagctt cactttgcct cgcctttcca ttgtgatatg atgaaggacg
     2461 aagccaataa gcaggctctg gggcaggttt tagagcgtgc tttaggcacg cagccgcaaa
     2521 ttcactgttt gttacagaaa cagggagcat cacctgaggc gacgacgacg gcaacggcaa
     2581 cggcccaaac tgaaaatgat caggatgatc tcgtccattc agctgtagaa atttttcgcg
     2641 gtaagattat agacgatttt ggcaaaaagt aaggaggcgg ttaaagatgt atggcaatat
     2701 gggtggtatg atgaaaaaag tgcaaaagat gcagaaagaa atggctcaac tacaggagga
     2761 actacaagag cgaaccctgg aagtgaccag tggcggcggt atggtacgag ttgaggtcaa
     2821 tggtaaaaag gaagttgtta acttgcaaat tgatcctgaa gcggtagatc ccgatgattt
     2881 agagatgctg caagatatga tcattgccgc ggtcaacgaa gggttacgta agattgatga
     2941 aatgatcatg accgaaatgc agaaagttac cggtggactt aatttgcctc cgggtttgtt
     3001 ataagcgcgg cggcgagcag tgttttatcc tgactcgatt gctaatctaa ttgaagcaat
     3061 gcaaaagttg ccgggagtag gtccgaagac agcacaacgc ttggctttct ttattctgac
     3121 tttaccccgt gatgaagtgg tgcaaatggc aagagcgatg gtaaatgcca aggacaaaac
     3181 acattactgc agcatctgtg gtaattttac agacagcgaa ctctgtgtaa tttgtgtaga
     3241 caaacgacgt gacagctcct cgctgtgcgt ggtgcaggat gcacgtgatg tactggcctt
     3301 agaacgcatg cgtgaatata aaggtctcta tcatgtttta ggaggggcca tttcccccat
     3361 tgatggcatt ggtcccgatc agctgcggat gcgtgaacta ttggcgcggc tgcaagatgg
     3421 gcgtgtcaag gaagtgattt tagcaacaaa ccccacggta gagggggaag ctacagcact
     3481 ttatgttgcc cgcttagtca agccgctggg aatacgtgtt acccgcctgg ctcacgggct
     3541 gccagtaggc ggcgatttag aatatgctga tgaagtcaca ctttctcgag cccttgaggg
     3601 tcgccgggag ttttagctgc agagcagaat atttcttaag gaacatgctg agcatgttcc
     3661 tttttgcttg tagtaagctt atttagagag aaaataagtg catgaaatca gcgcacagtc
     3721 atagtagagg gtcaaccatc atatagtgta gtacatgcta tgtagctggt tgaggaggta
     3781 acgtaatgga gaagaagcaa aagcatgcag gtccagcacc ctctactgta agatattttc
     3841 ttgcctcatt aaaggaagtt ttgcaagcaa gacaagctgc tgtgagtttt tttaatgagg
     3901 tgacagaacc agcagaggtg gatgctgcca ttaaggggct aactatctca gaaaacaaat
     3961 ataaagagtt gttacagcag gcacgccagg ctggttacca gattccagtt acgctaagtg
     4021 ggtttgagga aaggaggaat ttattttatg cctaaaattg atttaaatct ggtggtagca
     4081 gccatttttg gcttgtttat gctttattac ttaggtaaag cgctggcaac accagcccgt
     4141 gttgttgtac gcgtgctggt aaccggtgtt gtaggtgtgg ctttactttt tatttttaat
     4201 ttagttgcag gtttgtttag tatgaagatt ggtattaatg ccattacagc actagttgtc
     4261 ggttatatgg gtttgccagg cttagcaatg ttagtagtta ttcagacttt cctaggttag
     4321 cgcagcaaaa taatgctggc gttgtgtaag caaattaaat gctggcaaaa ggaattacct
     4381 ggtaatagca gaattaacta cataaaggag ggatgaactt tgagggaaaa atatttggca
     4441 gaatggcagc cagtagctga aaaatatgtt tcgccttggg gcaacgatgg cagtggacat
     4501 cgtatggtgg aacctaaaag agcttatcca gttagtgaac gccggaattt tgagttggtg
     4561 cttaatggtc agcagccggt ttatatgcct tttattacag atatgcttgc ttttagtcca
     4621 cgcctcattc cggataattt cgtccgtgcc tgggtcttgg aaatcaaccc gctgctgcct
     4681 ggttcaccgt gtgaaggtgg acctgatatg tttggtgtgg agtggcaata cgtaaaagtt
     4741 accggtgggt cgatggtcaa gcctggccaa ccaaaaatta aagacataag gcgctgggag
     4801 gaggagctta cctttcctga tctcgctgct tttgactggg agggtagtgc tcaagctaat
     4861 gcgccgcttt ttcatcccga gaggatgaca agagtgtggc tgatgaatgg cctcaatgaa
     4921 agacttattt ctttgatgga ttttgccaat gccatggtag cgtatgtaga cgaagaacaa
     4981 aagcagggcg tgcatcgctt ctttacgcga ctatgtgcat tctatgatga cttaatagaa
     5041 tattttcacc actattatca ggcagatgtg ttaatgttta atgatgattg gggtacgcag
     5101 cgaggtccgc aattttcccc tgccacggcc agagaaatgc ttgttcccta tatgcagcgt
     5161 atcgcagatt cctgtcaccg caggggaatg tattttgaac tgcattgctg tggcaaaaat
     5221 gagcagcttg tgccggcaat tgtggaaagc aatgttgata tttggctgcc ccaagagaat
     5281 attaacgatt tcgaattact ctatcgactt tgtggcgata aattactgct gggcattccc
     5341 acagattcaa ttccggcaat gacagacgcc gaagctctac agtctgctaa acgttttatg
     5401 gagaagtatg gccatgaagg gcatgttata gctaacggca cttttcctgt gcagcatccc
     5461 aaggtggcag aatatctgta ctgcctaagt cgtgaggtgt atcaagaaaa ataattatca
     5521 ctgcttgatg gtgcagttac ttggtagtct aattaatgct tgacgctgtg cttgcaggga
     5581 gatcatcgca aattagcaag ctaaaggcag caataggacc gggctccgtt gggaataata
     5641 tgctttgttt aggcggtttt gttgtggtac aataccattg agcaaaacta aggaaatgag
     5701 gtgtagcccg tgaattggaa aaagggggat ttgttcgtag ttgcagccgt tttagtagcg
     5761 gccttggcct tgtttgcgat cagagctcta cccgcagctg ctgagaatcg ggaactacgc
     5821 gtggagttaa atggcagcac gatagagaaa ataacactta aagaaaacga tgtgcaaacg
     5881 ctgaccatcc ctttggataa aaggggcgag gcggtggtgg aaatagagaa tggacgtgtg
     5941 cggatgctgc caatgtccaa agaattatgt ccccaggcta tttgctctca tgtaggctgg
     6001 atagaacatg ccggtgaggc gatagtctgt ctgcccaacc gtttagtttt aacagtggtt
     6061 gggggagctg cagaggatgt attggatgga gtggctcgtt aatgccgcgt aaagttatcc
     6121 tggtaagtgc ttgcctttta ggtttaaata ctaagtatga cggcacacat aactgtcgtc
     6181 cagaagtatt ggcttggcaa gagcaggtta acctgattcc tgtttgtccg gagcagctag
     6241 gtggtttgag tacgccgcgt tcgccaacag agattcagga tggcgatggc catgccgtgt
     6301 taaagggtgc ggctgaagtt aaaaccgctg ctgggggcaa ttgtacagat gcttttaggc
     6361 gtggtgcaga ggaagtctgg cagctagccc aagtcatggc tgcagcagga gctctattga
     6421 aagcacgcag cccatcgtgc ggcagtggtt atatatataa cggctccttt actgggcaga
     6481 ctaaggcagg ggacggtgta acggctgctt tgctaaaaat taacggcctg cctgttttta
     6541 cagaagagga acctcttcaa tttaaacagt ggttggaaaa tggggacagt tgacaactgt
     6601 ccctgcagtt gttacaatca agcaaattat aagagggggg tggcatgatg caaccggaaa
     6661 agttcggtaa aattggcatt acctttgatg atgtgctgtt ggttccggca aaatcagacg
     6721 tattgccatc ggaagttgat gtgtcgacgc agttaacaaa aagaattcga cttaacctgc
     6781 cgctgatgag tgcagggatg gatacagtaa ccgaaagccg catggcgatt gcgatagcgc
     6841 gcgaaggcgg cattggtgtt attcacaaaa atatgtctat tgacagccag gccgccgagg
     6901 tagataaagt taagcgttca gaacatggtg tgattactaa tccctttttt ctttcgccgc
     6961 agcatacttt aggtgatgcc gctgcactaa tggaacgcta ccgtatctct ggtgtgccca
     7021 tcacagttga aggaaaatta aaaggtatta tcacaaatcg agatctacgt tttgaaactg
     7081 attatagccg tttaattgat gatgtgatga cgaaggacaa gctcatcacg gcgtcagtag
     7141 ggacaaacct gcagcaggct caagaaattc tacgtcagca taaaatcgag aagctgccgc
     7201 ttgttgatga caattttatg cttaaaggtc tgatcaccat taaggacatt gaaaaagcta
     7261 ttcagtatcc tcgatcggcc aaagatgcta acggacgctt attggcagca gcggcggtag
     7321 gtattgctaa agatacttta gtacgggcta aggctttagt ggaagcaggc gtggatgtac
     7381 tggtagttga tactgcccat ggtcattcta ggaatgtctt acgcatggtg gaaagcctca
     7441 agaaagaatt ccccaaagtt gacttggtcg ctggcaatgt tgcgacaggt gctgcgacca
     7501 aagatctcat cgcagcgggc gctgacgctg ttaaagtcgg tgtgggacct ggttctatct
     7561 gcaccactcg ggtggtggca gggattggtg tgccacagat tacagccatt cttgatgccg
     7621 ctgaggctgc tgcagcttat gggattccca ttattgcaga cggtggtatt aagtattctg
     7681 gtgatattac taaggctatt ggcgccggcg ccgatgtagt aatgattggc agcctgctcg
     7741 ccggggcgga agaaagcccg ggtgggatcg aaatcttcca gggtcgcagt tttaaagtct
     7801 accgtgggat gggctcttta tcagcgatga aagaaggcag taacgatcgt tacttccagg
     7861 agaatgaaac gaagttcgtg cctgagggta ttgagggacg tgtcccatat cgcggcaccg
     7921 tcggtgatac agtctatcaa ttagttggcg gactgcgctc cgggatgggt tactgtggag
     7981 tagctaacat ccacgaactc aaaacaaaaa gtcaattcat ccgtattact agtgcctctt
     8041 tgcgtgagag ccatcctcat gatgtgcata tcactaagga agcgcctaac tacagtctct
     8101 cttaaccagc gatgaagctg cctcatgcat agcacaaagc aaatagtggc atgcttacag
     8161 gcagaggcga tggagagtga ggcgaaatta tgagtgagca gaaatgcttg atttgcagcc
     8221 gagaaactgc aacaggctta cgcatcaata catctgtact atgttgggat tgcgaagcgg
     8281 agcttttaca gacagaagtt acagatccta agtatgatct gtttgtcaag aaacttaaac
     8341 aagtatggcc gccccgtcca agcttgcagc atttagctat aaaataaact aaattgtgtc
     8401 tgatctctaa taatgccggt gtaaaccggc attattattt aagcttatag ttagatccct
     8461 ggcggtagtg tgttcgcacc agtaaaaggt actactttgg taaattgcag cagcaaagca
     8521 ggtttagagg cagtgcttgt ggaacaataa caacgttatg gaacaaacaa gagccccttt
     8581 gtgggaaaag ataactgaat ttaaacaaaa aaagggacgg gtttggctgc acgtaccaac
     8641 acatggtggt ggacgaggtc taccaccact tatgggacca attttttcgg cctatgctca
     8701 attggattta actgagctgc ctggtttaga tgatttgttt cacgctacgg gcgtgctggc
     8761 cgaggcacaa gatttaactg cccggctctg gcaagcgcga cgttcgtatt ttttggttaa
     8821 tggtgctagt gctggtgtaa gagcgatggt tttagcaacc tgtaatcctg gggatacgat
     8881 tctgattgcc cgtaatgcgc atggctccct ggcccaggct ttagtgtctg taggagttat
     8941 cccgcgctat ctgccggtag cagaagtgga gggctttccc cttaatgtga cggtggaagc
     9001 tgttaaagag gggctgcgac gctatccgca ggctaaagcg cttttgctta ctagtcccag
     9061 ctattttggt gtgacggcga atctggcagc gattgcggaa gttgtgcacc aaacgcaggc
     9121 cttattgctg gtggatgaag cacatggggg gcattttgaa ttttctgacc agctgcccac
     9181 ggcagcaggg cagcattgtg atttacgggt acaaagctgg cataaaacat taggtgccct
     9241 gacgccgggt gctgttttgc actggcatag aggtgcagta gacgaagatc gcttgcagac
     9301 agctctgcaa ggggtacaaa ccagcagtcc tccctacccg cttttggttt cactggactt
     9361 ggtaaggcag cgcatggcgc tggaggggaa agagctggta gggcaattaa tcgagcaggc
     9421 tctggcttta aggctggccg tggggaaaca ctttcctctg ctcaccccgt ctctggtgca
     9481 gtccctgggc tttgagctag atattactcg tgttaccatt ttaacggcac aagcaggtgt
     9541 ctgcggttta gccgtggggc agcaattagc acagcaaggc attgatttgg aactaataca
     9601 acccgactgc gttttggcta tcgtcggtcc tggctatcgg gagcagtgga cagctaggct
     9661 ggcaaaatct ttagcaggca tgcagctaac ttcagctaac ttgcaattac cagctttacc
     9721 accggtgccg gaattagtta tgacaccccg agaagcagct tatcgacagc atgttacaat
     9781 cccctatcaa gcagcggcgg agcgagtagc tgcagcgatg gtagtttgct atccgcccgg
     9841 tattccacta tgtctaccgg gggagcgtat tacctcagca ttaattacat atttaacagg
     9901 cgccctggaa cttggtgtta atttccgagg tctaactaaa gacgggcagc ttaaagtcgt
     9961 taaagaggtg acataaatgc aaaaaggata ttttattacc ctagaaggac cagatggtgc
    10021 gggtaaaacg acacagttgg cacggctgta tgccgcggcc agtgcagcgg gtctgaccgt
    10081 agttagcacc cgggagccgg ggggaacccc tgtgggcgat gctattcgcg gtattttatt
    10141 agatccccac tatggggaaa ttatcccgct gactgaagct ttgctctatg cggcctcccg
    10201 tgcgcagtta gtaaaagaag taattaagcc agctttagcg gcgggtaaat tggttttatg
    10261 tgaccgcttt attgactcga gtttggcgta ccagtcattt ggcggcggtc ttgatttcga
    10321 ttttgtgtgg cgtactaatc tggctgccgt tgatggttgt ttaccggaca aaacttttat
    10381 cctgaatatt aatccgcaag tagggctggc cagacgtggt gccgctgctg ctgaccgtat
    10441 ggagcaaaag tcgctggcct ttcatcagcg agtacagcag ggatttttac agttagcgca
    10501 gcaatttcct gaacgtttaa ccgtgattga agcagaacga acagcgcctg aagtgtttaa
    10561 agaaatctgg cagcaggttg ccgccgcagt aacttcactg cgtggaaatg tgtaattgtg
    10621 gtataatatt gctgataacg aggttatata atgatctgga atacaatcta cggacgccaa
    10681 gaacttgtcc aaactttaca aggggctatt gaggcagagc aaattgctca tgcctatctc
    10741 ttttatggtc cggtgggcat tggtaaaaaa accatcgccc aggttctggc gacggcctta
    10801 aactgtacag gagcggccag aaatggctgc ggcacatgtg aatcctgtcg taaagcacaa
    10861 aatggtaccc acccagacct gcattggctg cggccagaag gtaaatcatt aaaaatcgag
    10921 cagattcgtc agctcaaaaa aaatgcttat ttaaaaccac gagaaggaaa ttatcaaatc
    10981 tttattttag agcaggcaga tgccctcacg gcagaagctg ccaatagttt attaaaagta
    11041 ttggaagaac cacccagggg gtcgcttttt atcttattgg cagagcaccc ctctatgctg
    11101 ccggctacag tcgtttcgcg ctgccagctg tttgtcgtgc cacgtctcaa tcaagccaat
    11161 atcgcacgct tattgcagca gtatgacttg ccggctgccg aagtcgctga aatcgcggac
    11221 tgggcagagg gtattcctgg cagggccctg gctatgagca accaaactac ttggcgtaca
    11281 caatatacag cagcgataga tacactggca gctcttgctg cggctggcac agtgagtcag
    11341 ctggcagcca agctggaaaa gctagaagaa ctgacaacct ttttagatgc catgacgctg
    11401 gttttacgtg aagtcctctt atggcaaaca gtaggtgttt tagctaaaca agagcaacaa
    11461 cagcaaatct cacaacttgc cgaaacttgg tcagtccatg cttgtatcgc tgctttagac
    11521 gagatgctct tattacagca gtcattgcaa agtccggtta acaagcgttt ggcactggaa
    11581 aaaatgttaa ggagcctgaa ggaggttaga aatatagatg caaacagtag taggaattcg
    11641 ttttaaaaag gcaggcaaaa tatactattt tgatcctgat ggcatggatt tgacgtcggg
    11701 taattttgca atagtggaaa cgtcccgagg gttggagttt ggcgaagttg tggtcaaccc
    11761 taaacagatt cccgaggaag aagtagtaca gccgttaaaa aaagtcatcc gtgcggcgac
    11821 ggatgaagac tgccaacaaa tattagaaaa tcgtcaaaaa gaggaagaag cttttgggct
    11881 ttgtctacaa aagattgctg aacatgagct ggatatgaaa ctggtggatg tcgaatatac
    11941 atttgatcgt tcgaaagtga ttttttattt tacttctgac ggtcgggttg actttcggga
    12001 gctggtcaaa gatttagcct ctattttccg cacccgtatt gaattaaggc agatcggggt
    12061 ccgtgatgag gctaaaatga ttggtgggct aggaccttgc ggtcgagttc tttgctgtca
    12121 taccttttta ggtgagtttg aaccagtttc cattcgcatg gccaaagatc aaaacttatc
    12181 tttaaatcca accaaaattt ctggtgtatg tgggcgcttg atgtgctgct tgcgttatga
    12241 agcggatgtc tacgagatgg agcgggcaga gaaaagcgct aaacagtcag aagaaaaata
    12301 gctgtgtttt taagggggaa tcccccttaa tcttgcgggg tggtcaggac ccgcaggtcc
    12361 gtcgaagggg tgctggcgat tgctacaaaa tcagctaacc aacagtatca gagaatagtt
    12421 ttatgtactt tctttagcgt acctggctac tttaactccg ttagctgctg ctcactgggt
    12481 taggcctacc cctaaagaac aagcaagacg catcaataaa aaaataagaa gcgtcttgct
    12541 tgttaactgg gattatggct aatagtctgt gtatcaagtt cacgcgggag gagcgctaat
    12601 tcagaaggta tgagcaggtt gccttcaggc ctttctcctg ctgctctgat taatagatca
    12661 ctggctcgat ccaagacgtg gccgcctaaa aagacaagca gatcattact actgtgaaga
    12721 tgcgttagta agctgtcgat tgcgtccgcc aaatcaggaa aataagcgaa atcgatgtct
    12781 ttttctttta actccctcaa aattgctttt tcttcatgca gcctcacttg ttgcttactt
    12841 ttacagtgat acatgctgcg agtaatgaaa agttgacatt gggggttttg ttgtatccag
    12901 tgggctaatt cccgtgcatt gtattcgttg agtttaggcc caccacctcc gtaaatacca
    12961 tgtaaaagat aaattgagtc ggcagcgaaa tcttttatgg tagctaaagc agcttgaatg
    13021 gcggcaggat tatgggctgc atcatcaagc aaataacctt gcgcaagcgg aattacttcc
    13081 attttcctgc gtatgccggg gaaacggttg agggcctgtg ctacagcttg cagatcgggc
    13141 acaaaaagta aagccagcgt agctgctagt aaagcattag ccacgttatg cagcccaagc
    13201 agtggcagct gcactgtggc agaagctggg ctaattattt tttgatttag actgacgaac
    13261 tcctcattaa cttttaactc aaaattagtt gttgtctgca gctgtttgac gtttgtagca
    13321 ataatcatgg cattatgata atgtgtagca taagtgatat agtctacatg cccttcctga
    13381 gctaaagata gagcacttgg gtcatctgca ttaataacgg cttttgctcg ttccggcagt
    13441 tgggcaaaga agcctgcttt atagtccaga tagcggtctg gagtatgaca ggatgtcaaa
    13501 tggtctaggg aaagatttgt tagggcgccg cactggaagg ctctgccgtt gagttgtttg
    13561 cgccgcaaag catcaacagg aacttctatc agcaggcaat cacttgcttg tgccgcggcc
    13621 tgttgcatag cttgttctaa aaataccgtt cctttccaag agtctaaaac ggttggctta
    13681 agaccgcatt gttcaagaat gaaacccagt atgaggatag ttgttgtttt accattagtg
    13741 ccagttacac caagcagtgg gatgttgttt gttagtgggc tcatgacatt gctccttcga
    13801 tttggcatgc attttagtgt ttgtgtacac gatagttctt atgtatggta aagatgcgag
    13861 gtaagatatg aaaaaaggta ctctttattt atgcccaacg ccgctgggta atctcaagga
    13921 tattacgctg cgtgttttag atgttttacg ggaggctagt cttattgctg ccgaagatac
    13981 acgccgtaca cgaaaattac ttagctatta tgatcttcat acaccactta ctagttatca
    14041 tgaacacaat aaagcagcaa aaacatcaca gttgattgtt aaactccttg agggtcagac
    14101 gatcgccgtg gttagtgatg ccggtacacc cggtattgct gacccgggag aggaactggt
    14161 tcaagctgct atagcgcagc agattacggt aatagccctg cccggtccgg ttgctgcgat
    14221 cacggctctt accgcctcgg gattaacggc agttcctttt gctttttatg gatttttgcc
    14281 gacacatggt gcagaaagaa aagtaaagct tgctcaaatt atgggcgaag caaaaactgt
    14341 tattctgtat gaatccccgc atagattatg caagactctg gcagaactct gtgccattga
    14401 tcccaagcgt aaggtggttg taggacgcga attaacaaaa ctccatgaag agtatcttag
    14461 ggacacctta gaggctatgt ctgctcactt tgacagggtc aagcctcggg gggaatttac
    14521 tctgcttatt gccggcgctg agcccgaggc tgaagagccc acacaagatc ctctgcagtt
    14581 agcagaacat tatcttgaag aagggttgtc gacgcgggag gcagcacgga gggcagcaga
    14641 agtaacaggc tgtgcgcgca atgaaatata tcgtaaaatt atagaaaaaa agaaaagcga
    14701 gtaggttaag ttacctaaat cgcttttcag tgttgacact tttggcaatt ctataaattt
    14761 tttacaagcg ggacgttttt tccctattgc gcagacattt ctttgatgca ctcagcacaa
    14821 actaggcggc ctttataatg ttttacatta tcagcgttac cgcagaaaag gcaagcaggt
    14881 tcgtacttct tcaggataat ccgttcgctg tccacataga tctctaatga atctttaaca
    14941 tcaatgccca gcgtccggcg cagttcgatt gggattacaa cgcgccccag ttcatcaacc
    15001 ttgcgtacaa ctcctgtaga tttcaacaca tattcccccc gttcaacata cttcgacaag
    15061 atttagtata ccagcaattc ccagataaaa caagtatttt ttttgcaaat tttatgttat
    15121 caaggaaatt agacaaatta agcaatgacc gcgccataaa ttatatttat gactaatgcc
    15181 aactttataa catgtatgcc aaataaagtc agattgcttg cagaagaggc tattttcttg
    15241 ctataataaa cacattcttg attttacaat taagtacata tctctagtgc caatatctac
    15301 ataaaggaga atctgtatgg aaaagaaaac attctatatt actacaccga tttactatcc
    15361 cagcgataaa ctacatatcg gacactccta caccacggtg gccgctgatg ctatggctcg
    15421 ctttaagcgt cttactggct ataatgtgcg ttttttaact ggtactgacg aacatggaca
    15481 aaaaattgaa cgccgtgcgc aagctgcagg taaggagccc caacagtttg tagatgaaat
    15541 tgtggcttgg attaaaaatc tttggcaaac attagatata tcctatgatg attttattcg
    15601 tactacagag ccacgacacc aaaaagctgt acaaaaaatc gtacaaaagt tttatgatca
    15661 gggtgacatt tataaagcta aatatgaagg ttggtattgt actccttgtg aggctttctg
    15721 gacagaacgt cagctgcaag acggcaactg tcctgactgt cagcgaccag tggaactggt
    15781 agcagaagaa agttattttt ttagaatgtc taaatacgtg gacaggctgg ttgagcatat
    15841 tgagcagcat cccgacttta ttcagccagc ggcacgtaaa aatgaaatga ttaacaattt
    15901 tatcaagccc ggtttagagg atttatgtat ctcccggacc acatttagct ggggtatccc
    15961 cgtacccttt gatccagagc atgtgatcta tgtttggctt gatgccctta ctaattatat
    16021 tacggcttta ggttatatgt cagatgacga ccagctctgc caaacatttt ggccggctaa
    16081 tgtgcagctg attggtaagg aaattgtgcg ctttcataca atttattggc caatcttttt
    16141 gatggcttta ggtttggagc tgccagagaa agtgtttgga cacggttggc tgctgttgga
    16201 tgacggcaaa atgtctaaat ccaaagggaa tgtggttgac cctgaacttt tagttaaacg
    16261 ctatggctcc gatgcgattc gctattttct cctgcgtgaa atttcctttg gcgccgatgg
    16321 tcactttagt gaagaagcct tggtacagcg tctgaatttt gatctggcga acgatttagg
    16381 taacttatta aatagaacgc tggcgatgtt gaaaaagtat ttcgctggtc agattccgac
    16441 gcccggcgcc agtactcctt ttgatgagga attaaaaaat ctggctgcag aactgccggc
    16501 taaattagag tcgctgatgg atcaactgca gttttccaat gctttagcag aaatatggaa
    16561 gtttattgcg cgcgccaaca agtatattga cgagaccatg ccttggactt tagcaaaaaa
    16621 caaggaagaa gatctgctgc gtacagtgat gtacaacctt ttagaaagta ttcgtattgt
    16681 ttctgtattg ctgcagccct ttatgccgca aacaccgggt cgcatttggc gtcagctggg
    16741 gatagcagaa gagactagtg tccatacttg ggacagcatc ttgacgtttg gacagttgcc
    16801 gtcaaatggg cagacagtgc cgggagaagt cttattcccg cggctagagc taacaggaga
    16861 aggaggtgcg ccggtgcgtc aagcaactaa aagagataag ccagcacaag aaaaacaagc
    16921 acctgcggcc aaggaacaaa aaacagtgac agaagatggt ttaatctcta ttgacgattt
    16981 cgctcgtctg gatttacgcg ttgcggaaat aacagcggcg gaacccgtga gcggcgctga
    17041 taaattgctg aaactgcagg tggatgtggg aggagaagtg cgtcaggtgg tggccgggat
    17101 tgccaaacac tatagcccac aagagctgtt aggaaaaagg attctcttcg tggccaacct
    17161 taaaccagcc aaactgcgtg gcgtacgttc agaaggtatg cttttagctg cttctgataa
    17221 tgaaggtaac ctcgtgttgg caaccactga aacacccatt aaaacaggca gcaaagtgaa
    17281 ataatgattt cattaatcga tagtcatgca catctgaccg atgcccgttt ccaggacgat
    17341 tttactgaag tagtggcacg ggcgcaggca aacggggtta acacgattct caatgtgggc
    17401 tgtgatttac cctcatcccg tgctgttgtt gtgcaggctg agcaaaatgc aggtttttac
    17461 gcagctgttg gtattcatcc tcatgatgct agaaccgcca atgcaaaagt gctgcaggaa
    17521 gttaaagaac ttgctgctca ccccaaagtg gtagcggtcg gtgaaattgg gctggactat
    17581 tactacaatc acacgccacc tgcgcagcag cagcttgctt tgcgtcaaca aattcgtttg
    17641 gcacgcgaac tagctctgcc gatgattatt catgaccgtg acgcccatga ggatgtgctg
    17701 ttggcgctgc aagaagaaaa agcgcaagag gtgggcggtg tcatgcattg tttttccgga
    17761 gacttggatt ttgctaaccg ctgcctagaa cttggctttt atatttcttt tgccggcacg
    17821 gttaccttta aaaaggcggg ggccctgccg caggtggccc agcagatacc gctggagagg
    17881 cttttagtgg aaacagattg tccctattta gcaccagtgc cctatcgtgg tcagcgtaac
    17941 gaaccagcta atgtgctgca ggtagcagta aaggtggcag aactccgtca acttgaattg
    18001 gctcaagtag cagatgctgc cagtaaaaat gcccaacaac tattttcctt ggacaagttt
    18061 taggagaagt tagcaaaaat aggccacatc aagatacata gagataagag caaggagaga
    18121 ggggatgctc atggcccttc ccggctggtg taccaaagtt ttcggcacac ggactgtgga
    18181 aaaggcaatt ttgctgatag tgctgctagt cattacgagt gtggcaggga ttgctgccac
    18241 agcagctaaa cctgtttcct taagtgtaga tggtgaagtg caagcactta ccactcaggc
    18301 caaaacagtt ggcgctgttt tagcggaagc agggctttat gcccgcagtt gtgatgtctt
    18361 gacgccgcaa gcagataaaa aggtaacgca tgatatgaaa ataagcctag taagagctgt
    18421 tcctgtttac cttaaagtgg acaagcaaaa ccgcctcgta tatacaacat ctgcgacaac
    18481 cgcagaattt ttaacagaac agcaagttga attagcagaa actgattgct tgtctcctgc
    18541 aggagtatcg tgtttagaca atggaagtac agtgcaagtt atccgtatga cgactgattt
    18601 ggtgcaagaa gaaacggaaa ttccttttaa gacaacgcaa aagaaagatc ccaaactagc
    18661 tagcggtcgt aaaactgtgc aagtagaagg taagcctggt atgctggaaa aaacctatca
    18721 ggttgtttat gctgatggtg tagaagaaag ccgcgagttg gtagaggaaa aacggttaat
    18781 agagccggta aattgtgtgc tagcgattgg tacaaaacca gtaccggtca ttgccagccg
    18841 caatggtacc acaggtcgtg tttacgaagg tatggcctct tattatgccg acaaatttgc
    18901 tggtcggaag acggcctacg gtgatgcata taatcctgag gcctacacgg ctgctttccc
    18961 ggataaggcc cttcgcggca aaaaacttcg tgtcacctat cttaaaactg gacgtagtgt
    19021 tgaagtgctc gtcaatgact tcggcccaca caccaaagga aggttggttg atctttctgc
    19081 ggctgccgcc aggaagatcg gactgttgtc agcaggagtg ggcaaagtta aagtagaagt
    19141 gctgaagtaa aaaagcttgt ttcagtttcc ctggcaagct ttttatcctg gcggctaaag
    19201 ctttgctaac tgcgaagcca tttactttcc tagcagtgta aatgactgcc caccggtaca
    19261 agtaaataag tactggtggg tagtttattc aacggcagta atgggggcgg gggaggctgt
    19321 aaccaatgaa aaaaacaatc tgcatactgc tcgtggtaat tttattgcca cttgtcatgc
    19381 tcctcacgac tgtacaatta gttgtgaaaa atgatagctt ttttgctgag caatatatag
    19441 ttaataatgt ggcggccacg acacagattg aactggctga attaatgcaa attacagatg
    19501 aaattcaggc gtatttactt ggtcagcgcc gagacttttt gattcaagga gtagtcaacg
    19561 gcgaaagcag acaaatcttt aacgaacgtg aaatacaaca tatggaggat gtacaagtgt
    19621 tgtttgccaa agggctgcgc ttgcgtaact ttagtgctat catcctggca ttgttgttag
    19681 tttggctatg gttaaaagaa cgagtattct tttatcaagg tcttttggcc agtgcagtta
    19741 tcttttttat ggcagctgcc ctaggtagtt tcttactgta catgaatttt gatcgttatt
    19801 ttgtgctgtt tcacgaaatt ttctttaata atgatttatg gatacttgat ccccaaacta
    19861 gcatcctcat tagaatggta ccgctgaatt tttttatgag tacggttaaa cagatcggcc
    19921 tccttacggt aatgcagatg ctgctgttgg gtgttttagg ttttagtggc cgagtgttag
    19981 gacgtcgaca tcaggctcgt atttaggcac aatgtacgct ttagctggtg tgtgagcctg
    20041 aagcaacaaa gaaaatagta acgcgaagat tgtcgactaa tagtcagctt tttttaagta
    20101 cggctaggca gccagcttgt ttacacctta aatcgtgctg ctcttttctg acgcataaga
    20161 aatgaaaatg cggcaatgct gtggatgata gtattaacgc agcctaggcc ggtcttggtg
    20221 ataatatttt atggcctact cagcaatcaa ctttacgttt gagcgcagac agctgcgcgt
    20281 ctactactac cggggggtac tccggttttg ttttttacca gctaagatga taatgtattg
    20341 ctaacaggaa ttgcacaact tttatggaat gagaagaaac agagggggaa cggtatgagc
    20401 aaattatcga atccagctta cgtctctgaa ttactggcag gtcatggcat tcgtttaaaa
    20461 aagagatggg ggcaaaattt tcttgttgat gataatattt tgcagaagat agcagctgcc
    20521 gctgatctgc agccacagga tttggtgttg gaagtaggcc ctggtattgg tgccttaact
    20581 gaaaagctgg ggcaagcagc cggccatgtt actactctgg aaatagatga gcgtttactg
    20641 ccggtgctag aggaaacgct tgctgattat aagaatatcg agacgattca tcaggatgct
    20701 ttaaaagctg attatcagca attatgcgct gctggccctt taaagatagt agccaatcta
    20761 ccttataatg tggccacgcc gcttttttat ctgtggttga aaaaattccg tacctgcatc
    20821 aaacagctgg tatgtatggt gcaaaaagaa gtagcgcagc gtattgttgc tgctcccggg
    20881 ggtaaggatt acggtacatt atctgttatc tgccaatatg cagctgaggt gcagctggcc
    20941 tttgaggtgc cgcgcactgt atttttcccc cgccccgagg tagcctctgc tgtgctgaac
    21001 attaaaccta aacagagaac gctgctgaca ccagcggcag aagtacactt ctaccaaatt
    21061 gtcgaagctg tctttgcaca acgccggaaa actattctca atacactgca tgctgcttta
    21121 gggatggaca aagcagaaat attagtcttg ggtgagctgg ctgaattaga tttatcacgc
    21181 cgtggcgaaa cgcttgccgt ggaagaattt gcaaggctgg cgcagatgtt ttataataaa
    21241 aacaacgctg caaagtgaaa ggtgcacaga acgcatggat tttataagtc cctctcaagg
    21301 acagctcacg ttcgacgaaa catatcaggg aattacctcc tatgtagatg aacagccaca
    21361 tgaacaatac agattgatta taggcaccga ttctcagact catgccagtc aagatgtttg
    21421 ctttgtcact gctattatta tccaccgagt aggtaagggc gcccgctact tttatcgacg
    21481 tttgaataaa gcatacatga ataatttgcg tcaacgtatt tactatgaaa cgtatttaag
    21541 cctggaagta gcaatgttgg tgaccgccaa acttgctgaa aatggtgact cccgactaaa
    21601 tgtggaaata cacttggacg tgggcgaaca aggggcaaca aaagaactaa tcagggaagt
    21661 tgtagggatg gtaagcggca gtggttttcc ggcttttatt aaaccggact cctatggggc
    21721 ctccaaagtt gctgataaat atacaaaata aaaatacgcc ggcggggcgt attttttatt
    21781 tacatagtgc gaccggctaa aaattgtgcg agtaagtcaa aacgtcgctg ctcatgatag
    21841 ccaagataca attttcccgc atggtagcga ctgcgcggtt gatggttaat catacatagt
    21901 gcttcaagac aatcgccaat aatggtgtct gtaaccagct tattgtgtac ctcattttta
    21961 atagaggagc ctaattgagc atgaggaatc gagccggcaa gatctacttt aagctgatgt
    22021 cgcaccgcgt gaacaagtaa ggttggggga acggcataat cttcccaggg gactatattt
    22081 aaaagccgac gtgaaactag atggggccca tgtgaaggtg tgccaagtct aatctgctcc
    22141 gccagcgcca attgttgatt taccagatgg cgataataaa acaagggttc ttgaaattct
    22201 gtcagttcag tgtgatgggg gtaacagtcg aaaagcgcca gatccagcat tgcttgggtg
    22261 gctgtttcca gcagttctgt tacttctgct gtatattcac ccaccatatc accatcgaca
    22321 aataaaatgt aatctgcgcc actggtgtag gctagattag ctccgacagc tcgcggcaca
    22381 tctataccta ggggtacgct aaaagagacc agcgtgatac tttgcggctg cttctgttgt
    22441 gctatttctg cctctttctg cgtaagcttg ttagagccat taagaatgac aaaaatatgg
    22501 ttaatgagct tgatggataa caactgactt aagacacgac cgagacggta ttcttcatta
    22561 tatgcaggca caatggcgac gatcatttta tcacctccct cttattatat gaagaggtgt
    22621 aggcaggggg tgcagacttg cacaaatctt aactaacaat cataccataa aatgaataaa
    22681 gctgtgcccg aaaaagaaag gcaggtgtaa cagaatggca tttatggtag gggagattgt
    22741 gggacgtaaa tcgtatcaaa tggatttgtt atttaagatt gtggagattg atgaaaaaaa
    22801 aggcagggca gttttaaaag gtttagatat gcgcctgctg gcagatgcta atctttcaga
    22861 tttaagctac ccggagcagt cgcatttgca gaaatatcgt aaagaatctg atgaagcgca
    22921 aaaagattgt ctgcgcaaaa tttatcgtcg tcgcagcatg gagcgggagc agctacgcag
    22981 tgttagtggt agagcacccg aggaattttt ttctctaccg ggaaaaatac ttcaccttga
    23041 tggtgatgct gactatcttg gcgaatgtct gaaggcgtat gagtcgttag aaatagaggc
    23101 tgtaggtgtg ttcgcaccgg agaaggagca gcctgctcag gtttggaatc ttctgcatga
    23161 gcatcgcccg gatattttag taatgactgg tcatgatgct tatattaaaa ataaaaaaaa
    23221 tgattttcag gatcttaagt attatcggac ttcccgctac tttgtggagg gtgtgctgaa
    23281 agctcgtcag tatgaaccgg gtaaagatga tttagtgatc tttgctgggg cctgtcaatc
    23341 acattacgag cagcttttag ctgcaggtgc taactttgcc agttctcccc agcgtgtatt
    23401 aatccatgct ttagatccag tatttattat ggagaaggtc gcttttactt caattaatga
    23461 gtcagttaat attttcgaag ttattaaaag cacaatcact gggctagaag gtctgggcgg
    23521 tttggagaca cgcggtaagt ttcgtttagg gctgccgaaa tctccatact agaagtgcaa
    23581 acatcgtgcc ggtccacatt actggccggc gtgaataaat ttgctattga cagaatgcag
    23641 accgagcagt ataattgtct atttatcatt tgacagattg tatagggtgt gatagaataa
    23701 ataagaaatc ttgtctgggg tgatagcgtt tggcagcaac aaatgcactt atgcaaatta
    23761 ggcgtaacct tgaatcacac gtcggacaga aaatacgtct gcgtgctcat cgtggacgcc
    23821 gcaagactct ggagaaagtt ggcgtgttag aaaacacata tccgtcgatt tttatcgtgc
    23881 gggtagatga acaaaattat aaccagcgtt tatctttttc atatgcagat gtgttaacag
    23941 agacggtgga actaacatta atgcagaaaa agcggtaagt agcctaagct cgaaataaaa
    24001 gactgcactg aaggataaaa ccttcagtgc agtcttttct tattttgtag cacacatctt
    24061 aagctctaag gttgtgacag ccctttgagc agtgctcaga tctccatcaa tctcactggc
    24121 ctgttttgcg agggccaggc ttgccacttt tcctccgacg ctgttttccg gtttcccagc
    24181 ccggaaatta gagttgaagc cgcgtctcgc ttgtctttga gcaagcgact caggcagctc
    24241 aggctttttg atgtactttg tgctaaaaga catattttcc agattatgac catagatatg
    24301 ataaagacgc acttcactgc gcacatttcc tttggcacgg gcatataatt aatgggataa
    24361 agctacggga aagggggaag caaacttttt tccgttcttt gccaaaagta ggtagattag
    24421 atgaaaggag gcaaaaagat gggaaaagac gaatttctgg cgttggaatg tattacggat
    24481 ctcttgaaag ttgaacaggt tgtcggcgaa aatgcctcgc aaacatcagt ggtgcgggat
    24541 attgtcttac ctggaagggt tcgtaagatc gcagaagtcg acaccgagct gcgtgatgtt
    24601 aaaggccgcg tcatagaaaa taaagttgtc gttgaaggtg tcattcacaa acaaatcttt
    24661 tatgtagata gcgaaaccgg gcagatgaaa gaattcacgg tagctgacga gcgctttgtg
    24721 cattttgtcg atgttcctgg agctatgcct gggatgaatg tacaagtgca tgttcgagca
    24781 gaatttgttg gtcatgactt tatccgtgaa gtgaaagcat acagagacgc ggagctagta
    24841 gaagatttag acgttgaagc tgaacatgaa aagcacaaga agaaagttga ctccctttac
    24901 cgtcaaactg ttattctgga gatcttcgct aaagtcacag aatcaatgca gcttgatgtg
    24961 gtagtagatg tcaatggcgt tgacaggaac aatgtgatga aggaactgtt gaaagtagaa
    25021 agtgtaattg gggaaaatgc ctcgcaagtt tcagtgcaag cagaaattga ctttgatcgc
    25081 cctgcccgca aaatcaagaa tattgatacc caggttcgta atattacaac gcgtgtgatt
    25141 gaaaacaaag tgatcgtcga gggtattctg cataaacaga tttattttgt tggtgcagag
    25201 gatgatgttg tgcatgagct ttctgtagat gagaagttta ctcactttgt ggatgttccc
    25261 ggtgcattcc ccagtgccaa tgtacaggtt cacccacgtg tcgaatttgt tgatgtggac
    25321 atcaatgctg atgatccgac tcgtggcaag cagacggcca tcattgacat ctttgccaaa
    25381 gtgacggaaa cagtccaggt tgaagtggta acggatatta tgggggccgg tgtccgcaaa
    25441 gaactgctta aggtctcaca agtcatcggt gaaggctcta aacaacataa tgtcgttgct
    25501 aacctgacag caccagcacc ggctaaaaaa atggctgggc cgccagagac acgctttgaa
    25561 aatcttgaaa cacgtgtcat tgaagataaa gttgtcattg aaggcgacct cgtcaagaat
    25621 gtttattttg tgcgtgatga cgacgaggca gaagtaaggg aatttgaagt acgtgaacac
    25681 tttacagtat ttgtagatat tccaggcgca atgcctgggc atgttgtgca agtgtatccg
    25741 cgtgtagaat ttacagactt cgaaatcgac cccaaagatc gcactcgcat tcgtcagact
    25801 tcaattatag agatctttgt taaggtgaca gagcacgtac aactagaagt ggtagtcagc
    25861 tgtgaaacag ggcctacacc ttcggtgacc atttatgtag tgcagccagg tgacacactc
    25921 ttcttaattg ctcaacgttt taatgtgtca ctgcaagcta tagaaaaagc aaacccgcaa
    25981 attaccgacc ccaatgtgat tttcccggga cagcaaattt tcataccccg tcgttaatga
    26041 taaaaagtag catcggccga tgctactttt tttaagcaat tagatcttct ttagtaacgc
    26101 ttaatgtata cttgcctccc gacaatgagg caagtttttt atcgggatag gaaattatat
    26161 caaccttgaa ttactttctt tcctatccga tggggatttt taagggaaaa cgctaatcgt
    26221 catttttgtg tgtgtcaatc tgcgtctaca ctgcatacaa tagatgggag gcgtgtatat
    26281 gataaaccaa gcaaacaaag taaaccgcat tattgcactt atacaagacg gccaggaagc
    26341 ggtggtgagg atacccttag ccgttaccct gcctggagaa gtagaggagt ttaacgagcc
    26401 ggaaattacc gtgatcgaga ctacagcaac agccctaact gaagaaatat ttctcgaaat
    26461 tactctgcag caggacacat attatgtggt taaagaaaca gggctggtaa acatagcaac
    26521 tactgttcat cagttcactc aactcttgcc ggcggcaggc agccaacctg gacacatggt
    26581 gcagttagca gtagatccgc actttgagga gggctggtta gctaaagtaa gcaaggaaga
    26641 ggaaaaacag ctgacgatct taacaggtac ctgcagactt aatgctgctt ttcaactttt
    26701 agcagaagag gaactagtgg tagaacaaac agacaccctc acgggcaaag cacttacaga
    26761 gccaatcaaa atagaaactg tacattctca tttcacagaa gtcgttgatc tctctttacc
    26821 gctgatccta acagaaacac cagacagcaa ggtccaggcg agggcaaaat ttattaatca
    26881 acgagctgcc gccatgtgtg gttgggtgaa agtagaagga gaagttttag ttacagtttc
    26941 ctaccagtta gctaagagaa ttataaaaag ggaaaactat gtttaccccc taaagaaatt
    27001 ctttgaacac caaacttcta acatcgaaat ggacgcaaat gtcgcagggc gcactgaaat
    27061 attaacctgt cagctagatc cagcaggaaa aggagtattg cggggactat tacaccttag
    27121 tggtacacta atcaaggtac aaaagatcgc tgatgagctg cgaagtttta ctagtgacag
    27181 ctatgccccc catcactata agaagcattt tgagctggaa gaggtagtag gggtaggctc
    27241 ttcacaaacg ctgattgaac aggaaatttt ctttggccgc aaggtgaaat ttgttaggga
    27301 accagtagct gcagaagtcc gtcatcttca acatgaaata atccataata aggtaattgt
    27361 ccgcggtacg ctgcataaag agctctttgc cgtagaggcc aaaaatagca cagttttcgt
    27421 acacgatgtg caggaaaagt tcgttcattt tgttgatgta ccaggagctg cccccggaat
    27481 gcgtgcccaa attgcagcca gagtagagta tgtgcgcgta gaagttaatc cagaccagga
    27541 aacggcacgc caggtagcga ttatcgagat tcgggttaag gtactgcggc gcatcaaaaa
    27601 agaccatcag ccgccagtta agccacccca tgacccctgg ccaaagccgc cgcattatcc
    27661 tacaccacct cccgcaccag agcagcgaat ctatatagtg cgttccggtg acagtgtgtg
    27721 gaaaatcgca caaaaatttg gcgtctcgat ggaagtcatt attgccgcca ataacttgca
    27781 aaatcccaat ctcatttatc cgggtcaaaa attaatcatc ccactgtaat ggtgctttta
    27841 accattacac cgcattaaat aaagagttgg tgttatcgcc aactctttat ttaatatttg
    27901 cctgtagcca tcactggacg ctgtatttac tttttgccac tttgctagtt gttgcttata
    27961 ataagttgtg aagatatctt aacaatctta ataaaactta aaggagttgt taacgttgcg
    28021 aaagaagtgg acaaacatag ttctccttgc gcttttactc acaattatta ttgttccctt
    28081 tgctgctagg gcagaagcgg ctgccgttcg ttatacttat cggtacacac taactaaagg
    28141 gtggcagctt gtttccgcac ccgctaccgc cagaattact ttcagttgta aatatgcttg
    28201 ctatcgtgta aaagcaggtg acacgctaga gagtattagc cgacgctttg acaccaccgt
    28261 ggataaactg ctgcaattaa acagacttaa tcgggctgaa gtaaagtcag gtcaacttat
    28321 acttatcccc ttatgttcta ccgacccgca gccccaacca gagcctaagc cagagcccca
    28381 accagagccc aagccggaac cccagcctaa gccggaaccc cagccagagc cccagccaga
    28441 gccccagcca gagccaaaac cagagcccaa gccggaaccc aagccagagc caaaaccaga
    28501 gccccagcca gaaccccagc cagtgactgg tttaaagcaa gctgaacaag caatgttgaa
    28561 tctgctgaat gcggaacgca ctaaacttgg tttacagccg ttacaagttg atctggagct
    28621 ggtcaaaata gcacgcttaa aaagtcagga tatgatcaat ttaaattact tcagtcatca
    28681 atcaccgact tatggttcac cctttgacat gatgaaagca gcaggtatct cctacagaac
    28741 tgctggcgaa aacctagcag gtgctccgac tgtagagcgg gcacatacag gcctgatgaa
    28801 ttcacccgga caccgtgcta atattcttaa tgccaactat actcggattg gtattggtgc
    28861 agtcagcggt ggtccttacg ggatgatgtt tacacaaatg tttgtaggac gttaagatag
    28921 agcaaaaggg gctgacttag caacaggtca gccccttttt tcgtagagtt tattaagtta
    28981 ctaatacttg gggatgagca gcattttgcc gggatgcaga tacttaggat acttaatttt
    29041 attaaaggca atcagctgtt ccggtgtaat gtggaagcgt ttagcaatac tttctaccgt
    29101 atcaccgggg cggacaaaat aaatttcctt atcaatatgt tcgggaattt tgacaatttc
    29161 ttcacgctca aagataatta ccggcgtgcc aatgtaagtt agcggatata tttcttctac
    29221 atcttcatta tacatacgta cacaaccagc tgtcgccgct gtgttaattg tccaaggatt
    29281 aaaagtgcca tgtatgccat gtctttggta ggtaaaggcc atccaacgtg tgcccagacc
    29341 aggatgcggc tgtgggtttt tctcataaac atggtattcg ccaagtggtg tgggagtatc
    29401 cggtttgcca atggcgatgg ggtaagtttt atagagctga ccttgacgat aaaacatcaa
    29461 ttttttcatt ttcctattta tctcaatacg agtcatttcc agacctccaa aagttgttta
    29521 ttttaccctg aaatttatca agtattgtgt tttatgatat gtacagtcag ctaattttgc
    29581 taatattgct gtttgaaaag caattagctg cttgtgggaa aaaacgaaat taattattgc
    29641 cagctatatt caaataaaag cgggagaaac tattttagac cctaaagttc aaaaaaagga
    29701 gtgaggctaa tgagtccaag acgtgaaaga attatgtctg atgctctaaa ggaagagtta
    29761 gccgaagagt taggcgttgt taacgtagtg cgccaagaag gttggggcgg tgtttcttct
    29821 cgcaattgcg gaaacttggt taagttggcg atcgaacgcg ctgaacggtt tctaatgaat
    29881 ggacaatagg gtgacagaca gcgtaccggt tttcggtacg ctgttttata taatgaagaa
    29941 aaaggagatt tagtcctgat gtggaataga tagcataatc atataaattg caggttcaaa
    30001 actagcaggc ctgaccagat tagatgtctt gttgttcagg ctagtaattg aggtaaggac
    30061 tagctatttt aaaatcaaga ttctagactt gggtaaaata acagcactac gcagtgaatg
    30121 tggagggaaa aaggtgaacg aattacgcac tttagaagca gttaaacagc tgaaaatagc
    30181 agaagatcgt gtgctttatt ctgcaaatga tgcagaaata atagctggtg ccaccacaga
    30241 tatttatttt gtgcgcactt tagaaattct acgtgcggct gggaagtatg agacagaggt
    30301 tacggcagaa atattcgcca gcagagcggg tgtgctctgc ggggtagacg aagcaatcaa
    30361 tttgctgcga cttgctaatg tagaagtttg gtcgcttaaa gaaggagaag aatttgcgca
    30421 aaaagatgtc atcatgcgca tcaagggccg ctatagcgat tttggtgttt atgaaacggc
    30481 aattttaggt atattggcta gttcctgtgg ttgggcaacg gcagccagaa aaattaagaa
    30541 gttagcggga gaccgtcctg tcctttgctt tggggcacgg catgtacatc cagctgtagc
    30601 ttctgtgatg gagcgggcag ctatcgtggg cggtgccgat ggttgtagtt gtattctcgg
    30661 tgctaaactg gccggaaggg aaccttccgg tactgtgccc catgctgtaa tgttggtggt
    30721 aggtgacact ttggaagtag ctcagcttta tcatcaaact atgccgcctg aggacccccg
    30781 cattatgctt gttgatactt ttaaagatga ggccgaggaa gcgctgcgtc tagcaaactc
    30841 tcttaaagaa aatttgcagg gcatccgttt agacacgcct ggtgaacgcg gtggtgttac
    30901 tccaggctta gtgcgtgaac tgcgagctcg tttagatcag gctggcttta aacatgtaaa
    30961 attgttcgct tcgggtggta ttgatgagga gcgtctgccg cctttactag aagccggtgt
    31021 agattctttt ggtattggtt cctatatttc tcgagcttcg gccattgata tgaccatgga
    31081 tattaaagaa gtagaaggta aggctattgc taaacgcggc cggataccgg gtgttaccaa
    31141 cgcaccaagc ttagaaaaag ttttatagca gatatttaat ttaataaatt cggcatagtc
    31201 gtaaattacg gctatgccgt tttttgcttt taaaataact agacaacaat acagccgcca
    31261 aagatcagca gcagaaaatc accgcagaaa gaccagcagg ctaaagcaat actttactgt
    31321 gtctatcatg tctgtgtata ggataacttt gacaaggaaa atctaattag tgttgtgaga
    31381 aaaatggagg tgctatcttt tgtcaggcga acaaagtggc cagttagtga taattggtgg
    31441 tgccgaggat aaagaaggtg aatgtataat tctgaagcgc tttctctctt tagcaggagg
    31501 cagaaaggcc cgcttggttg tactctctac agctagtgct gatctgcatg cgggagaaca
    31561 atataatcgt ctcttccgca aattgggggc tgctgacgtc agagtacttg ccatccaaac
    31621 tagagaagaa gcaaatcagt cacaatatca tgatggtttt gcagagtcca caggtatttt
    31681 ttttacaggt ggtgatcagc tgcgcatcac cagcatctta gggggtagta tcctgggcag
    31741 cgccctgcaa gagagttatc gtcagggtgc ggtaattgca gggactagtg ctggtgcttc
    31801 tgtcatgagc gagacaatgt tagtgggggg agctgatgat gcgactccca gcaaagagat
    31861 tattcgtatg gcaccgggac ttggcttgct gaaggatgtt gtcgttgatc agcattttgc
    31921 ccagcgcggc aggattggcc gcttattggc tatggttgcc cataacccct atatcctggg
    31981 ggtggggatt gatgaggata ccgctattgt ggttaatgat agtgcagaat ttgaggtaac
    32041 agggtctggc acagtaacgg tgctagatgg gcgggaagta actcattcca atgtctcaga
    32101 ttctagtcct gctcagccct tggccttaac taatgttaaa gtacatattc ttgcccaaga
    32161 ttatcgcttt aatttgcagg aacgctttgt gaggatgccc gcaaaataag gaggacgtat
    32221 agtaaaatga agatcgtgga cttgcatttt tacaccgggc gtaacattta cagccactat
    32281 ccggtaatga gaatgctgct agatctggaa tctttccaga attatcgcac agactgtgac
    32341 cctacttttg ccagtcgcat attgcaggca ttgcctacat tatgtgaaca tacatgttca
    32401 cgtcgccgtc caggcggttt catagaaaga gttaaagaag gcacatatct tggtcatgta
    32461 gttgagcatg tctttttaga actgcagcat ctagccggta ttggcgcaaa atatggcaaa
    32521 acttataccg gtgatggtcg ttttgtggaa attatcagcg agtaccgctg tgagcaagca
    32581 gcgcgtattc tggcagaagc ggctgttcag tatgtccgct ctctggccac aggtgtcaca
    32641 ggagaaaatg gtcaggtggc tgctagttta gctgcggcaa aaaaggcggc gtctctatat
    32701 ctgccagggc caagcacggc tgcggtgtta gcggcggcca ggcgacggca gatcccctac
    32761 cagcagctcc agcctggaac aagtttgtat cgtttgggga caggtaagta tcaaaaacgt
    32821 atccaggcta gcctgactaa tgagactggc tgtattgcca ctgatatcgc gtgtagtaaa
    32881 ccactgacta aaaaaatact cgcgcagcat ggcttgcctg tacctaaggg aagaactgta
    32941 tcttctgcag aggaagcagt gctggcagcg caggaaattg gatttcctgt agccatgaag
    33001 ccagataacg gcaatcaagg gaagggcgtt tccctaaact taaatagtat ggcagaggta
    33061 caaaaggctt tttctcgagc agctgtcttt agcccctctg tgatggttga ggcttgcctc
    33121 gatggtagac attatcgctt gttagtggtt aaaggccaac tagctgcggc tgcggagcgc
    33181 ttgcctgcct ttgtgacagg tgatggaatt cataccatta gtgaattagt aagtgaagag
    33241 aataaaaatc cgctacgcgg agacgggcat gaaaaaccgc tgactaaaat ttcgcttgac
    33301 ggggtggcag aagaagtgct gctgcgtcaa ggtatggttg cccagacagt ggtaccggcg
    33361 agcgttcgtg tttatctgcg tgataatgcc aacttatcaa cgggggggac ttcttgtgat
    33421 gtaacagatg aggttcatcc ccaacagatt gctttagccg tgcaggctgc tagctatatc
    33481 gggttagatg tagcgggagt tgatctggta atgaaaagta tcgccttacc gccagcagaa
    33541 caagcaggcg gcatcattga agtaaatgca gcgcctgggt tacggatgca tttgtatcca
    33601 agtgtgggga aaaaacggga tgttggtgaa aagattgtta gtgctctttt tccctttgag
    33661 caaccgacac gcgtgccggt gattagcatt actggtacta acggcaaaac taccactacc
    33721 aggatgttag aatatgtaat gcgtcagcag ggattattta cgggaatgtg ttgtacagac
    33781 ggcctttact ataatggtca acttgcgcgt tcaggtgatt tgacggggcc tgacggtgca
    33841 gggtctgtgc ttgcccatcc cgaggtagaa gtagctgttt tagaaacagc acggggtggt
    33901 attgtccggc gtggtttagg ctatgaacgg gctgatgtgg cagttatttg taatgtacgt
    33961 gaagatcatc tggggcaaga tggcatagaa agtcttgagg atttagttca tgttaaaagt
    34021 ttagtggcag aagctgttta tgaaaacggc acagttattc taaatgcgga tgaagcaaac
    34081 gttactgaaa tagcggccag ggtttgggaa agaattattt attttagccg gcaggaagat
    34141 aatatcatcg tgcgtcgtca tctgggcaaa ggcggccggg cactttttat ccgccgcggt
    34201 gccatagtgg cggcacaagg aaatagagtt gtactggtgg gacgggtaag agacttcgcg
    34261 gtaacttttg ctggtcgtgc acagcaccaa gttgaaaatc tcttggcggc cctagctgca
    34321 tgttggggta ctggcttatc gccacgacag gcaggcttct atctgcgcaa ctttacttcc
    34381 tcaccgcaag acaacccggg ccgggctaat ctttatcagg tgggttctgt gcaggttctg
    34441 gttgattatg gccataatcc agatggtttt gccaaaattg gtgatctggt gagaaaaata
    34501 aatactaaac gcactttggc ggtaattggt gtgccgggtg accgctgtaa tgaactcatt
    34561 tttacagcag gtaaaacggc ggcccagttt ttcgatcatt tattgattaa agaggatgcc
    34621 gatttacgcg gcagacgccc tggtgaggtt actgccctct taagagaagg cgctttagca
    34681 gcgggtatca agcttaactg tattacagaa atagctcagg agacaaaagc tgtacgcact
    34741 gcactgcaga tggcggcagc gggcgattta gtagtggttt tttatgaaga actggagcct
    34801 gtcattgccg agattattgc cagccaacag caacaaaggt cactgctgac agcgccaaaa
    34861 gaaaaaaaag aattatctta cgatagccct gctatgttaa cctagcagga atttgtcctt
    34921 tgctcacgaa tatacaagcc atatgaacag ttcagaacaa cagacgctag taatcgaaca
    34981 ggcatatgcc aaaattaatt taatcttggc ggttacaggc aagcgcattg atggctatca
    35041 tgacctgcag acagtttttc aaagcttggc actgcatgat accttaacat tcaccgctaa
    35101 cgaaagcgaa gtgcttacct taactacaaa taatcccagc cttcctgccg ggccagataa
    35161 tttaatctgg cgtgctgcag aaaggctaat ggagttttat aaagttaaaa aaggcgtggc
    35221 gattaatctg gaaaagagga tacccctagc cgcaggttta ggtgggggca gcagtgatgc
    35281 tgctgctact ttacggggac tggttcgttt ttggcagctc ccctgcgagc cgcaggtatt
    35341 atctgagata gcggctgaat taggttccga tgtgccctac tgtttggcag gtggtacagc
    35401 actgggtaca ggtcgagggg agcaattact tcagcttact ccctgcccgc acttttatgt
    35461 cgtcctggca aaccccggat ttccagtttc tacagcgcaa gtctaccagg acttgcggac
    35521 gacagagttg gcatcctctg ctgcgctgca cggaatgctg cgtgctttag cagagcaaaa
    35581 ccaaactgcc atgatgcacc atgtggctaa cacgctggaa aaagccactt ttcgtcttta
    35641 tcctcaagtt aaagcgctta agacggaaat ggccagtgca agtgcagctc ttatgtgtgg
    35701 cagcgggcct acagtttttg ctttattttt aacgtcagca gcggctactg ctgtacaagc
    35761 agctttacag caaaaaggat accaagtgtg gttgacggaa acaattcagc agccggaggt
    35821 agaaaaatgg tcgatgtagt tattttggct ggaacagcca agcaatcaga actgacaatt
    35881 agtgaaaagg tacaaaataa ggcctttgtg caaattcatg gacaagcgat gctaagttat
    35941 gtactagacg ccctgcagca agcaagctca gtaaaccgca ttgccatcgt cggcccgttg
    36001 gcagaactac agactttaat cggcgcatcg ccacagttgt ttgctgtggc ggaaggtgat
    36061 accattgttg agaatctaca gctcgggttc gcagcattac aacccaagca gcactgttta
    36121 gtggtcaccg ctgatgtggc ttttttgacg gcagctgcgg tggaagactt tcttgccgct
    36181 tgccagcctt atgagtcagc tgtttattat cctattgtag cgcgagaaga taatgaccgc
    36241 cgcttcccag gagtaaagcg cacttatgtt aaaatacacg atggtgaatt tactggtggt
    36301 aatctctttc ttgtcgatcc tgcgtgctta acaaaagtcc taccccgact agaacacttt
    36361 tttgcgttac gtaaaagccc cttgcgttta gctggagcct tgggccttgg ctttgtggtt
    36421 aaactcctga caaaacgttt gaccattgca gaactggaaa agcgttttta tgctttattt
    36481 gctgtagcgg gtaaggctgt tatttctcag tatgcagaga ttggtacaga tgttgacaaa
    36541 ccggctgatc ttgaattagc ccgtcaacac ttaaaaactc agctgtaagc tgcggcaaac
    36601 aaaacactag cagaataagg gtctttagcg ataattgtat gtgagtaaaa tcacatgcag
    36661 gaggtagcgg caggtaactg gcgcaaacaa tatttagcaa ttgcaaatat tttcccaata
    36721 aaaagcagga attgtcaaac ttatgtggaa atagcaaaac gtacatagtt gtggcgggga
    36781 aggcggtgat tagcatgaga gtgaccgacg tcagaattag aaagttaaac cacgatggaa
    36841 agatgaaggc aatcgtctca attactttag acaaccaatt cgtagtgcat gatgtacgtg
    36901 ttattgaggg taataatggg ttgttcgtag ccatgccaag taaaagaaca cctgatggtg
    36961 agtttaagga tatcgcccat cccattaact ctgaaacacg cgaaattatt caatcggcag
    37021 tgttgcttgc ttataacaca gagttggaaa gagaattagt cggtgcataa atggagcagt
    37081 tatgctcctg ttttttttgc gttattttgc ccttaagtca agctcctccc tgcctaggtc
    37141 ggatctggtc ttacatttag tcaagctgct cagctagcag tatcaaagaa gcagcaggaa
    37201 ttgtaagtgc tgtttttttt acaaaaaatg tagcaatcta agggcagaaa gggtacaata
    37261 aatggaagaa catcctagga ggggtaagtt tggctaaccg agcagctgta gtcttagcag
    37321 caggagaagg aaaacgtatg aaatcggcat ctcctaaagt acttcactct gtttgtggca
    37381 aacccatgtt agagcatgtg gtgagcgcag ttagtagtgt ttgttctgaa attattgtag
    37441 ttattggaca tcaaggccat gaggttaagc aggcttttgg agagagagta agctatgttg
    37501 agcaggcgca acagctggga acaggtcatg ctgtgctgca ggcagtaccc ttattgccaa
    37561 cttcgggtac tgtttttgtg ttgtgtggtg atactccttt actttcagcg gagacgatgc
    37621 agaacttact ggaggctcat caaaaagcgc aagcaggtgc aacaatttta acagcatgtc
    37681 tgccagagcc ttttggctac gggcgtatca tcagagacaa tggacgtgtg tctaaaattg
    37741 tagaagaaag agatgctacg gatgaagagc gtaaggtttg tgaaattaat accggcacct
    37801 atgtttttga tgcgcaatta ttgcgggagg ctctagcgaa tctacataca aataatgctc
    37861 aaggagaata ctatttaact gattgtattg aacaaataat tagtaaagct cacttggtta
    37921 caacttgtat tttagaggat caccgcttgg cactgggcgt gaatgatcgc ctgcagctgg
    37981 cccaagcgca aagcttaatg caagagcgta ttaaccgaac attaatggct gccggcgtaa
    38041 ctatgatcga tccacagtcc acttatgttg atgcgggggt cagcatcgct tcagatactg
    38101 taattatgcc taatacagtg ttgagaggtg ctacgcaaat tggtcatcat tgtgtaatcg
    38161 gacctaactc cgaactcatt gactgccaag taggtaatga agtcgtcttc cgccactctg
    38221 tagccaccag ctgtcgcttg gcagataagg ttacagttgg cccctttgcg catctacgtc
    38281 cagaaactgt cttggaaaat ggtgtcaagg tcggtgactt cgtagaaatt aaaaaatctc
    38341 aggtggggga gaataccaaa attgcccacc ttagctatat tggcgatgcg caaattggca
    38401 gcgatgtcaa tatgggcggc ggcatcattg tcgtaaacta tgacggccgc aaaaagcacc
    38461 aaacagtgat tggcacgcgc gccttcatcg gctgtaatag caatttggta gcacccgtga
    38521 ctatcggtga aggagctttt gtcgccgcag ggtctacaat taccaaaaac gttccggcag
    38581 gagcactgtc gttagcacgt gcgcagcaag ttaataaaga aggtcgtgcg acgcgtctgc
    38641 taaagaaaaa taatgacgaa taatgacgaa ctagcaggaa taagtgccgc gctaagcgaa
    38701 gaagattgag ttgtaagaat acggtcagga ggttatggcg tgtatcgagg aaagaaaatt
    38761 catatcttta gtggatcagc aaatttacag ctgaccgaag aaatagcgca acatattgat
    38821 cttcctttag gacaagccga agtaaagcgt ttcagcgacg gtgaagttaa tatttgtatt
    38881 ggagaaagtg tgcgtggctc caatgtgttt attgtgcaac cgatttgttg tccggcaaat
    38941 gaaaacctaa tggaactatt gattatgatg gatgcgctaa aaagggcttc ggctaagagc
    39001 ataaatgccg tcttacctta ctacggctat gcacggcagg atcgaaaagc aagagcccgg
    39061 gatcctatta cagccaagct ggttgccgat ttacttacta ctgccggtgc taatcgtatc
    39121 attacgatgg atctccatgc tggccaaatt caaggcttct tcaacatacc ggttgatcat
    39181 ttaaaaagtt taccgatttt ggcaaattac tttttacaaa aagaatttac tgacggagta
    39241 gtggtatcac ccgatgtcgg cggtgtcacg cgggcacgag ctttggccga gcgtctgcag
    39301 ctgcctattg ctattattga taagcgtcgt ccagaagcga atgtagctga aataatgagt
    39361 atcattggca atgttaaagg taaaaatgtg atcatgctcg atgacattat tgataccgcc
    39421 ggcacaatta ctttaggcgc acaggcactt attgaaaagg gtgcaaaaga tgtatatgcc
    39481 tgctgcaccc accctgtatt atcaggtccg gccctacaac gtctagctga ttcaccaatt
    39541 aaagaaatag tggtaactaa tactattccg caacggacag aagacaatgg aaaaatcaaa
    39601 gtgctttccg tggcaccact aataggtgac gccattattc gcattcatga ggaattatct
    39661 gtttctaaac tttttgatta agtcaatttg actgaaaagg taagttcggg gtataatacc
    39721 ttggaaaata ggagaaggag gatatatgat ggagcgtatg caagttgtag cacaaccaag
    39781 agcagccgcc acaaaggggc agttaaatgc acttcgtaag gcaggctctg ttccaggtgt
    39841 ggtctatgga agaaaaagcg aacccatctc tgtagcggtg gacagtaaag atattatggc
    39901 aatcttacaa tccccctcag gggctaatac attggtagac ctgagtgtag atggaaacaa
    39961 agcaacagta atgattaaag atttagagcg ggatttttta gtagcggacc gttacaccca
    40021 tgtcgatttt ttgcgcattt ccttactcga taaattggag gtacaggtcc ccattgcctt
    40081 gattggtgac gcacctggtg tcaaagatgg cggcatttta cagcattccc tgcgggaagt
    40141 aacgctaaaa tgtctgccta ctaacattcc tgatagtctc gagcttgata tttccggact
    40201 tgctcttggt gacagtctca cggtggctga tttagcagtt ccggctgaca gcgatctcct
    40261 ttctgaagca gatgaggtca ttgtttctat tgttgcccag cgtgtggaag aagaagctga
    40321 aacaactgat gatgccgaag tagaagatgc ggcagcagaa acagctgact ctgaagctgg
    40381 ccaagagtaa gtatgtaaga tgtttatcat actcggtctt ggcaatccgg gtcctcaata
    40441 tgcacgaacc cgacataatg ttggtttccg ggtgctagat cagcttagtg ccgccaccaa
    40501 aataccctta tataaagtgg gataccatgc cttttgggga aagggtacca tggcaggcca
    40561 aactgtgatt ttagctaaac ccatgacgta tatgaataat agtggactag ctgctgctgc
    40621 tttagcccgt cagtttaagg tgactgcaga taaaatactc acgatctatg atgatttaga
    40681 tctaccgctt ggtgcactgc gtgtacgcgc gcaaggcagc agcggtggtc ataacggcat
    40741 caaatcgctg atttttcact tgcagacgga acacattcct cgcctgaagg tggggattga
    40801 ccgccctagt gagcaaaacg tgatcgatta tgtgctgcag tctttttcgg cagcagaaga
    40861 agaagcttta gccccagtat tgaaagatgc agtttctgca gcgcaattat ttgtccgtga
    40921 gggtattcag gccacgatga attcttttaa ctccagtccc taaaaatgaa ttttctttct
    40981 ccttatttaa cccgcctgtt gagggcgggt taaataatct aaaggcagtt gcacaaactg
    41041 tttcacgata tcgcctggag cccagcgtgc gatgtataaa gcttttacct ctgcttcata
    41101 ctataagaac gtagattttt tctgctgttc tgctgtatgg taaagaggtg atagcttgct
    41161 gataacttgg atctgccgta attgcgagcg tcaagtcatg cgcctgaacg tcagtgaatt
    41221 tgattctcgg gtggcagcgt tgacggctca ggctggtgac gatataatag aacgtgacca
    41281 ggaaggaaat ttagtgattc acctactgtg tgagcactgt ctggaagtag cttaccggga
    41341 ggatgaaagt gaaatcaact ttatacgcgg accggagttg cattgagaag ataataaaag
    41401 aattgcgaat tttgcacacg tatgctgcta gaggccttgc tgtcataaca aggccttctt
    41461 tgcgtttgta tactgaccgg aggactgccc aatgaagaat attgctgctg cgattgccca
    41521 gatgtcgcaa ttacaaccac tatttgaagg tctgcacaaa acaacgcgca gccaactcct
    41581 ttatggactt gatgaaagtg ctaggcatat ggtgatggct gccatttatg aacaaacaag
    41641 acgttcagtt attattgtta ctgctgatgc ggtgcatgct aataaagttt atgaggatct
    41701 attagctgtg tttggcgaaa ataaagtttt tttgtttccc ggtaaagaat tgttatacta
    41761 tcatgacttg ctgtctgata gtggagatac cgcggcacag cgacttcacg tgatgaaaga
    41821 actagctgcc gacaagcagc caattgttgt gacgactgtt tctggtttag tagagaaaat
    41881 gtgcccacgg gagcagtggc aggcggcatg ttttactgta aaggttaacg ctgagctggg
    41941 gatggagcag ttattaaaat tgctcgttac tgccggctat gaacgagtag aactggtgga
    42001 agtagccgga cagttttctg tccgtggggg gatagcagat attttcccca ttggtgaagc
    42061 cacgccctat cgaattgagt tttttggcga tttaatcgaa tcaattcgca gctttgatcc
    42121 tttgacacag cgttcacagg agtctttgca aagtttgcag ttagctccgg tacgggaagt
    42181 tatcgtcggc gaagagcagc agcagcaagc tttaaccgcg ctggcggcag aacagcaaaa
    42241 gctggctgcc cagaagcgac aagagatccg cggagggggc caattagcag aaaaactggc
    42301 gcaggaaatg gaaaagataa aggagggttt atactatccc agtttggaaa ggctcttatt
    42361 atacttttac cctcaaccgg cgacactgtt agactggttt cctgcagagg cactaatctt
    42421 tctcaatgac cctcaacgct gtgaacaggc tgtagaacaa ctgaagcatg aactaggcga
    42481 aattcaaagt actctttttg cccagggtga attgctggca catcaggtag agatgacctg
    42541 ggattatgag gaagtgctct ccttgacaaa gcagcaaata gttgcttttg ccctttttac
    42601 gcagcaagga ttccctgtgc cttatcggcg cactatttcg ttttctgcca agcctgtgcc
    42661 ccgcttttta ggtcagtggg agctattttg ccaagaggta gagcaatggc gccaacttgc
    42721 ttatcgcgtt gttattatca cctcaacacg gcagcgtagt tcaggtatta ccgatatttt
    42781 ggccgaacgt aacatcccca gcgtctattc tttatcacaa cccgatttgg ctccccgtac
    42841 tgttaccctt ttacatggca gcctggagag cggctttatc ttacccgaac taaaattagc
    42901 ggtgttggct gaacaggata ttttaccaca acgtaaaaaa cgtcgtcggc tgaagggcaa
    42961 ggaaggcgtg cgtgtggggg actaccaaga gctgcaggtg ggcgactttg tggtccatga
    43021 gcagcacggt atcgggcagt atttaggcct acgtacgctg gaagtgagtg gtattcagcg
    43081 tgactatttg tatattcaat atgctggtaa tgataaatta tatctgccta ttgaacagat
    43141 agatgtagtg cgcaaatatc tcggtgcgga aggcaaaagg ccgaaactat cggccttagg
    43201 aggcggcgaa tggagtcgcg ttaaagcacg tgtacagtca tcggttcagg agttagccaa
    43261 agagctgctt gcgctttatg ccgctcggga ggctgaacca gggcatgctt ttgccgcaga
    43321 ccacgactgg cagcaggact ttgaagcagc ctttcccttt gaagaaacag aagatcaatt
    43381 gcaggcgatt aaagaaatta aagaggatat ggaaaagagc cgtgccatgg atcgtttatt
    43441 gtgcggagat gttggttatg gtaaaacaga ggtagctatg cgtgctgctt ttaaagccat
    43501 tatggatggg atgcaggctg ctttcctagt gccaacgact gttttagcgc agcagcacta
    43561 tcggactttc ctggagcgtc tggaaggttt cccggtcaag gtcggtatct taagccgttt
    43621 tcaaagcaca gcccagcaaa aagtcacttt gcagggttta gaagagggtt ctatcgacct
    43681 cctagttggc actcaccgta tgctctccca ggatgtgcgt tttaacaagc ttggtttact
    43741 ggtcgtggac gaagagcaac gctttggtgt ccggcataaa gaaagaatca aaatgctgaa
    43801 aaagaatcta gatgtcctaa ccatgacggc gacaccaatc ccaagaactt tgcacatgtc
    43861 cttagcaggt gtaagggaca tgagtgtgat tgaaacgccg cctgaagatc gttatcccat
    43921 tcagacttat gtcttggagt attcggacag cttaatccgg gaggcaattt tacgtgagct
    43981 ggggcgcggc gggcaggtct actttgtaca caaccgcgtg caatcaattg agcgctgggc
    44041 tgcacagtta aggaagctgc tcccggaagc aaaactcgct gtaggacatg gtcaaatgcc
    44101 ggaggggcgg ctagaaaaga ttatgctgga ttttctcgac ggtgagtatg atgttttgct
    44161 gtccaccacc attgtagaag ctggtttgga tatacctaat gttaatacga ttattatcca
    44221 tgatgcagat aaatttggtt tagctcaact ttatcaacta cgcggtcgtg tcggtcgttc
    44281 taaccggatt gcttatgcct atctaaccta tcaaaaagat aaagtgttga cagaagtggc
    44341 agaaaaaaga ttacaggcca ttaaggagtt caccgaatta ggttctggtt ttaagattgc
    44401 cctgcgtgat ttggaaatcc gtggttctgg aaatatcctc ggtcctgaac agcatggcca
    44461 tatgatggct gtaggttttg atatgtatat gaaattgtta gaggatgcca tcaatgccta
    44521 taaaggtacc aagaaggaac aaaaaatgct gcctcgcatt gaagtagagg cggatgccta
    44581 tttgccggcc agttatattt cggatacacg acaaaagatc gttttctatc agaaagttgc
    44641 tgctgtagaa acggcggagg aagtggcaga agcacgggaa gaattgcagg atcgttatgg
    44701 ttctctaccc aaagctgctg acaatttact gcaggtagct ttaattcgtt tactggcgtc
    44761 agatttggag attatgacca tcacagagga gcggcgcgaa attatgcttc gttttgcaac
    44821 gaagacaccc ttagccagta ggactttaca gatcgtggct aaaagacacc aggggcgttt
    44881 aactatggct atgggcaaac aattcgtgtt agctttccgt aaacaattta aagaagaccg
    44941 ggaaaagtta attttcctca gccagctatt aaacgagctg aaaattctct tagcgaaaca
    45001 ggcctaaaca gatcatctaa cagcacaagc gaagggaagt agatgttctt gtcacaaaaa
    45061 cacaacatga aatttacgct gggtttactg cttactcttt tgctgctatt gagcggctgc
    45121 cgtggccgtc aagtggtggc ggaagtaaac ggtgacacaa ttaaacgtgc tgagcttgat
    45181 gattataaga atgttttatc atttctcatg cccgatctag cccccatgct caatgaagca
    45241 aaggataggc aagctttaga agaaattatc ttgcagacac tgattgaaaa taaactatta
    45301 gtacagttag cggctgaaag ggaaatcgcg atcactgacg aggaattaac tgcagctgtc
    45361 gagcagcaag cagaaacaat tagcaccatg tcaggtgtcg acgacttaac gcagccgaga
    45421 gaagagctaa acattacaga tgatcagtta aaagcattta tcgccggcac gctgtatata
    45481 gagaaattaa tcgccaagat cacgccagag ctagagccaa aagaaattga cgattttcaa
    45541 agtgagcagc cgacggcggg agtaatgttg gccttaagtc aaattttagt gcagacagaa
    45601 agcgaagcag aaattgtccg tacacgtctt ttagctggtg aagagttagc aatagtggcc
    45661 cagttcttac ctgcgccgac agctttaatc aaacagggcg agcttggctg cgtccctgcg
    45721 gcaactactg cttatcccga atcatttatg actgctgcca atcagctggc agcaggcgaa
    45781 atttcagctc ccgtacaaac gggcttaggc tggcatatca ttgtgcggca tgagcgtcaa
    45841 gtacccacca aggaggaagc agcggccttt ttagctaggg agcgtttagt gactatcttt
    45901 gacgagtatt accagcgtgc cgaaattaag cttcgttaag taagtctttt tacttgagca
    45961 aattagctga ctttatattt tatcaggcag ttaatgtgta gccctgccca gcttaaggcg
    46021 cggaaactgc atagagtgag ctttcttgaa gtccaatcat taaatgaata aaagacttcc
    46081 ctgcgatata tactatgact agaggttaag actgtaattc tatgcagaaa aaggagggga
    46141 agtctgcttg aaggcaacag gaattgtacg tagaatcgat gatttgggac gtgttgtcat
    46201 tccgaaagag attcgtagaa cacttaaaat ccgcgaggga gatccactag aaatattcgt
    46261 tgaccatggc ggcgaagtga ttctgaagaa gtattcgccc attggtgaac ttggagattt
    46321 tgcgcaagaa tatgccgatt ctctttatga agctatgggg catattgcgc taattgccga
    46381 ccgggatacc attgtagctg tcgcgggtgc accaaaaaaa gaatttatga acagaccgct
    46441 ttctcctgcc gtagaaaagg taatggaaga tcgcaaaagc gtgctcatcg gtaacacggc
    46501 tgagcataaa ttttacaagg aactcgataa ggatgatgct cccaagttta tggtagaggc
    46561 tatcgcacct attatttctg gaggggatcc gattggtgcc gtaatcctgg gctccaagga
    46621 tcaagaagtt aaaatgagtg acttggaaat gaaacttgta gaaacggcag ccggttttct
    46681 cgccaaacaa atggagcagt aatggttgtt taaactaaaa taggagatga ctaaggagca
    46741 gtgacagtgg ttgctgctct tactaattat gctgcttgac gattactatc cccctgtgag
    46801 gggttttttt ttgcttaagc aggatgaagg ttgcctgggc aaggtataat agtaaaatat
    46861 tatat
//
LOCUS       DASRMS010000175        14485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_586719, whole genome shotgun sequence.
ACCESSION   DASRMS010000175 DASRMS010000000
VERSION     DASRMS010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 14485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14485
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586719"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            225..1298
                     /locus_tag="VFC74_07645"
     CDS             225..1298
                     /locus_tag="VFC74_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HZK25244.1"
                     /translation="MDQGNHTTGKRKWKQLSESERYKIEALYEQGLTPAQIGCALNPQ
                     RDRRTIERELKLGLVEQRQMNPSNNKYAPMYVVELVYKADRAQMVRDERASNKGRGLK
                     IGNDHKLADYIESRIKDEKWSPDVVIGRLKMKGWGFKTTICTKTVYNYIDRGIFREVT
                     NKDLWVKKNGSKKRDYKKIRTVALNNKNGKSITERPEAANDRSEPGHWEIDLVVGKQG
                     TKPVILTLVERKTRKSLYVLVKNKTQKEVVAAIKRARRRVGGDFSAVFKTITADNGSE
                     FLDSEAIKKAAKCGEVYYAHPYSSWERGSNENGNRILRRFVPKGMNIGKLTVKQLQRI
                     EDWVNNYPRKIHGYKTANEMMVA"
     gene            complement(1551..3284)
                     /locus_tag="VFC74_07650"
     CDS             complement(1551..3284)
                     /locus_tag="VFC74_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1634 family transposase"
                     /protein_id="HZK25245.1"
                     /translation="MNLKQSRRKNGRVYLSIEKSYRDKVTGNPKARTIQSIGYLDELE
                     KEYQDPIAHFKEVARKMTDEENNKKKLTLTFNMDEQLVQGTDDRKNLGYAAILKIYHE
                     LGLHRFLNNRARNEDFKFNTNSIMMLLVISRLLSPGSKKKTFEEKGRYFERFNFSLPD
                     VYRALSHYAKIAKDLQQFLNEQITEKYGRDTKTVYYDVTNFYFEIDKPDEVRKFGPSK
                     EKRPNPIIQMGLAMDANGIPLHYELFPGNKLDKETFRSVIGEVRKNYDTGRIVVVADM
                     GIITGDNIYYLTGGKNRNGYVFSFSVRGATNEFKNYVLDNEGYAGTNGKPAGEDAEFK
                     IKSRRIAREINVTMKSGKKAKKTVYEKQVVFWSKKYAVKAKAEREELVKKALDLVANP
                     QKYNKATSYGAARYIKHLEFNKNTGEIFEGKKKPYFDDNKLAEHEMYDGYYAIVTSEM
                     QMTDGEIIDTYRGLWEIEETFKVTKGTLEARPVYVSREDRIEAHYLTCFIALVIIRLI
                     QKKMDRRYSPKMIIECLNKIASSNEQDNLYLFDYRSEISDDIGNVLGIDFTRKRLRLA
                     DIKNILANCKK"
     gene            3579..3911
                     /locus_tag="VFC74_07655"
     CDS             3579..3911
                     /locus_tag="VFC74_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HZK25246.1"
                     /translation="MDNCIFCKIVSGKAPADIVYENEDMIAFRDINPQAPTHVLVVTK
                     QHFQYLDELPTHDLQLAGKILLTVKEVAQKLGVTGGYKVVTNCGEPAGQIVPHLHFHL
                     VAGKKFLP"
     gene            4052..4237
                     /gene="rpsU"
                     /locus_tag="VFC74_07660"
     CDS             4052..4237
                     /gene="rpsU"
                     /locus_tag="VFC74_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012064148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HZK25247.1"
                     /translation="MTQIKVGKNETLDSAIRRFKKQCAKTGILAEVRKREHYEKPSVK
                     RKKKSEAARKHNKKRFG"
     gene            4330..5637
                     /locus_tag="VFC74_07665"
     CDS             4330..5637
                     /locus_tag="VFC74_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HZK25248.1"
                     /translation="MWRRRVLALLLLLMFLIVPLGGLQAAETAEKVYVIKLEGEVEAG
                     LYHFLQRAFTEAADNGASAIIMELNTPGGLVNSAKDIRDLIYDSGVPVYAYVRHSALS
                     AGAFLALSCRELYMAPGSTIGAAQIQNLSGEAVDEKIISAWEAEMRTVAQNQSKDPQV
                     AAAMVRQEIAIEGLVSDKQLLTLTSVEAEEIGFTDGIFATRAELLTALGLPGAAIYTV
                     KVSPAESLARFVAKPVGATLLLTIGLAALVIEIFMAGFGAAGVVSILAFALYFGGSII
                     AGFAGNEVIILFIAGIILMLIEAVIPNFGVVGISGIVAMITAIILSASTAEQGARIFL
                     TSLLLTAAVVAVAFRYLKRSKLWSQIILQYAETKERGYVGPTDYSSLVGKTGQTLTPL
                     RPVGAVEIDGKRIDVVSEGNFIAKSSAVIVVKVEGTRVIVRPQ"
     gene            5659..6648
                     /gene="floA"
                     /locus_tag="VFC74_07670"
     CDS             5659..6648
                     /gene="floA"
                     /locus_tag="VFC74_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496556.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HZK25249.1"
                     /translation="MPELLITLGLIVLGLALLFSFVPVGLWISALAAGVKIGIFTLVA
                     MRLRRVPPAKIINALIRSSKAGLDLKGDQLESHFLAGGNVDRVTNALIAAQRAGIELG
                     FERAAAIDLAGRDVLEAVQVSVNPRVIETPTVAAVAKDGIEVKAKARVTVRANIERLI
                     GGAGEETIIARVGEGIVTTIGSSETHKAVLENPDSISQTVLNKGLDAGTAFEILSIDI
                     ADVDVGKNIGAQLQTDQAEADKRIAQAKAEERRAMAVAHEQEMKANVQEMRAKVVEAE
                     AEVPRAFAQALREGKLGIMDYYNLQNIEADTQMRDSIAKLGGGSDKDDNKNKK"
     gene            6667..7143
                     /locus_tag="VFC74_07675"
     CDS             6667..7143
                     /locus_tag="VFC74_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25250.1"
                     /translation="MEKIALLLIFLVFSAVRTLAENQAKKPQQKTTIPSSRNRPSRPF
                     DQPLRKLEQALREAEAVLSAKQERPQRAQAKRETPNDMYSGEGVSLEHPSPAPREMGS
                     LNVAPPLQSTVLPVETLMLQEGFMGDKEEVLRGMVYAEILGPPKAFRHLKRPSWSK"
     gene            7396..8763
                     /locus_tag="VFC74_07680"
     CDS             7396..8763
                     /locus_tag="VFC74_07680"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZK25251.1"
                     /translation="MSRGGEVLKLCKERRFIVPILTLCVVILMVATVYSYHAGFGYVV
                     VIDGEEAGFLSKADLGELPVFIAQLTDEAQAAFQMQVLLNENLDFVEARRPGEHQSTA
                     VVKDEVRQRLSFSTYGFVLTIDGKETVALRSEDAYDKVLRIMKQAYLAAKEDIILKDY
                     AIQEEIDFIKRPVQPDEIVTVQEAANILLTGLPRNQTHLVARGDSFWSISREHNISVA
                     ALAEANPQIKNQQIKPNDELNLITVEPLVNVLVVEEITVTEKIPFQTQYLKDKKMYTS
                     QTKVKTNGQMGEKEITYLVTLANDEEVERTIVKETVLSEPVDKVVAKGTATAPKSGTG
                     RFMWPLANGGTITSTFGSRWGKMHYAVDIAAAGGTPIRAADGGVVKTSSYNGSYGNLV
                     VIDHGNGYSTYYAHNSRLMVGVGERVAKGQTVSLMGSTGNSTGNHVHFEIRKNGTPIN
                     PLKFF"
     gene            8871..9152
                     /gene="yqfC"
                     /locus_tag="VFC74_07685"
     CDS             8871..9152
                     /gene="yqfC"
                     /locus_tag="VFC74_07685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfC"
                     /protein_id="HZK25252.1"
                     /translation="MGNSKLTKWRDRLVDAMELPKEIILNLPKVTIIGNVQCYIENHR
                     GVIEYSSERVRVAINSGELIVSGQDLFIRYMANEEIAVDGSINAVQYEL"
     gene            9171..10394
                     /gene="yqfD"
                     /locus_tag="VFC74_07690"
     CDS             9171..10394
                     /gene="yqfD"
                     /locus_tag="VFC74_07690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YqfD"
                     /protein_id="HZK25253.1"
                     /translation="MFILKLRYFITGYLIVAVQGRNTEKFINLAIRQHIALWDVHRSP
                     EGASLKVDLDSFFELRHLAKQTGCRVQIIRKAGLPFFFNRLTRRRGLLAGLCLFFIAL
                     YVLSSFILFIDVQGNENLETAYIRELAAQAGVKPGLFKERLAKNQVTSQMLIAEPKLE
                     WVGLQIQGTRLVIEVVEQIKPPVEIDTHAHLVAAKDGLVTDVLVIVGEANVEVGDTVR
                     RGQLLIAGYLSPEPQYVPEEEQAIVRIPVRARGEVTARVWYEGYGEAELTEVIRTRTG
                     SHMSGWSFLVDGQSILRLGRNNIPYHHYDTETTSKLLLERIISIPVEIVTETHYEVVL
                     QEKQLTFEQGVELATERARKLAELQLPPGELVENVTVKEVELNKEGFVGVQYILETSE
                     NIAEEEIVNGGEATS"
     gene            10391..11371
                     /locus_tag="VFC74_07695"
     CDS             10391..11371
                     /locus_tag="VFC74_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HZK25254.1"
                     /translation="MRYNENSITIPVDSNEEIKQLLGRFDQHVTLLDRYFKVRIIPQG
                     NAIIIEGKQDEAEKVALLFRELRWLLQQGQVPTLPDVEYAIELIRTGQAERLRGLFGD
                     TVFLTPRGKRIKPKTIGQKEYLDAIRKRDIVFGIGPAGTGKTYLAMTMAVKALKERSV
                     QRLVLTRPAVEAGENLGFLPGDLQDKVDPYLRPLYDALYDLLGSDVYHAYLEKGMIEI
                     APLAYMRGRTLDDAFIILDEAQNTTVEQMKMFLTRLGYGSRAVITGDITQIDLPRERF
                     SGLRQVQSILKDIPGIDFIYLTKNDVVRHPLVQKIINAYELHDQEQERSD"
     gene            11374..13500
                     /locus_tag="VFC74_07700"
     CDS             11374..13500
                     /locus_tag="VFC74_07700"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HZK25255.1"
                     /translation="MGFTSRVKEQLKQNSFLSHQPRRQRMLLVILLYLALLFVLFFTL
                     KSNKLDLELGKPSPRRIVAEWEALDGYETERLREEAAQTVAESFDYDPVVLSRAEERA
                     ITFFNQVQEVRELELEDGEKMAQLIAAVEVELTEPLALVLLQARQQDLLDMQSQVREV
                     LQVILQQGVKPAGVETAQRQASQEINFLIFDQDQKRIMVRIAEAVIEPNMIYNATTTN
                     QARETAREAVQAVRILEGTEIIQERETVTQRHLEQLEALGLLRNRSGGIPMFFGLAAI
                     LLIIFITVSYYLYIYQKAIYHDIVRLLLLGLIILLTLTFTIAANYFSGYLVPVAMGAF
                     LIAVLLNAELAVLLTLAFSVFVVLITGNDFRYMLVAMFGGLIACFSVTDLSRRSDLTK
                     AGLYVSAVNVVAVFGLFLYTGNLRFGYDILREFGVSIFAGIANGLFSAVMAIGLLPYL
                     ESGFGLTTAVTLLELANPSHPLLKRLLMEAPGTYHHSLLVANLAEAAAETVGGDPLLA
                     RVGAFYHDIGKLKRPYFFIENQMGGANPHDKISPNLSTLIITSHIKDGLEMAQKANLP
                     QVIQDIIYQHHGTSLVSFFFQQAERNQDSKEIIREENFRYEAAIPQTKEAAIIMIADA
                     VEAAVRSLSKPAVGRVEAIVRKIIKDKLNDGQFDQCDLTLKDLDTIAVTLVKILSGIY
                     HTRIEYPEKELKAEIERGRKNGGSVK"
     gene            13481..13945
                     /gene="ybeY"
                     /locus_tag="VFC74_07705"
     CDS             13481..13945
                     /gene="ybeY"
                     /locus_tag="VFC74_07705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HZK25256.1"
                     /translation="MAVLLNNELKEKLKSTMLTAMESISAFALAEHNLPKTTEISLTI
                     CDNHTIQLINKQWRDVDTATDVLSFSLLTPGEDLVIAGELLLGDIIISLERAREQAAE
                     FNHSVEREILYLFTHGLLHLLGYDHEEPAAQQAMRLAEEKLLLAVGATRDEL"
     gene            13935..14312
                     /locus_tag="VFC74_07710"
     CDS             13935..14312
                     /locus_tag="VFC74_07710"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HZK25257.1"
                     /translation="MNFKHKSFKESIYCALCGLADAWRSERNMRFHTAAAVAAVAVGG
                     WFRLTPMEWLFLFSAVFLVMTMEMLNTSLERVVDLFTREYHPLARLAKNAAAGAALLA
                     AIYAIIVGFLVFGPYVAESFNLF"
BASE COUNT         4044 a         2827 c         3584 g         4030 t
ORIGIN      
        1 ccataaagca cctcgtggaa tcgtaatatt ctgtactagc ccagttgttt tcgtaccaag
       61 attgtacact agattcgaca gccacgcaat gctaatctgt ttctgatatc gtcgccggca
      121 agttgtaaaa tgaattgccc atataaataa aaaaaagacc cattccgggg tcaaaccagt
      181 tatgcttaat ttgtaaagaa aaaagcgact ggaggggaac cggaatggat caaggcaatc
      241 ataccacagg caagagaaaa tggaaacagc tttctgaaag tgagcgctac aaaattgagg
      301 ctctgtacga gcagggtctg acccctgcac aaatcgggtg cgccctgaat ccccagcgag
      361 accggcggac gattgaacgg gagctaaagc ttggcctcgt ggaacagagg cagatgaacc
      421 ccagcaacaa caaatacgcg cccatgtacg ttgtcgagct agtgtataaa gcggacaggg
      481 cgcagatggt gcgcgacgag cgggcgtcga ataagggcag agggctgaag attggcaatg
      541 accataagct ggcagactac atcgaaagta gaatcaaaga tgagaaatgg tcgccggatg
      601 tggtaatcgg caggctcaaa atgaagggct ggggattcaa aacaacaatc tgcaccaaaa
      661 cggtctacaa ctatatcgac aggggtatct tccgtgaagt tacaaacaag gatctgtggg
      721 tcaaaaagaa cggcagcaag aagagggatt acaaaaagat acgaacggtc gcactgaaca
      781 acaagaatgg taagagcata acagaacgtc cagaggcggc caatgatcgg agtgaaccgg
      841 gccattggga gattgatctg gttgttggta agcagggcac gaagccggtt attctaacgc
      901 ttgtggagcg caagacgagg aagtcgttgt atgtactggt gaagaataag acgcaaaagg
      961 aagtagttgc ggcaatcaaa cgtgcacgac ggcgggtcgg cggcgatttc tccgcggtgt
     1021 tcaagaccat cacggcggac aacggcagtg aattcctgga cagcgaagca atcaaaaagg
     1081 cggcaaaatg tggagaagtt tattacgccc acccttacag cagctgggag cgtggaagca
     1141 acgaaaatgg caaccggatt cttcgacgat ttgtcccaaa gggtatgaat attgggaaac
     1201 tgactgtcaa gcaattgcag cgaatagaag attgggttaa taattaccca cgaaagatcc
     1261 acggatacaa aactgcaaac gagatgatgg tagcatagga aatgtaaaag tccccctgcc
     1321 ccctggaagg cactacacta ccgaaaaaga cataactggt aatatatggt gggcaattaa
     1381 acttgcaatt ctcctcccat catgcaacag ctaggtaatg tttgctagcc gcggataata
     1441 gtggtataat cctgactttg acagcaaaaa aagcgaaata gaggccgcaa cccttgtaaa
     1501 taaagggttt gcggccttcg tgtttttgca aaaatatgta ggggacacaa ctattttttg
     1561 cagtttgcta aaatattttt tatgtcagcc aggcgaagtc tttttcgcgt aaaatcgata
     1621 ccgagaacat tgccgatgtc atcggagatc tcacttctgt aatcaaacag gtacaggttg
     1681 tcctgctcat tggagctggc aattttgttt aggcactcaa taatcatttt gggcgaatag
     1741 cgccgatcca ttttcttctg gataagcctt atgataacaa gggcgatgaa gcaggtgaga
     1801 tagtgtgctt cgatacgatc ttcacgggag acgtataccg ggcgtgcctc aagtgtacct
     1861 tttgtgactt tgaaggtttc ttcaatttcc catagtcccc gatatgtatc aatgatctcc
     1921 ccatcagtca tctgcatttc actggtgacg atagcatagt agccgtcgta catttcatgt
     1981 tcagcaagtt tattgtcatc aaagtatggc tttttctttc cttcaaaaat ttctccggta
     2041 ttcttattaa attctaaatg cttgatgtac ctggctgcac cgtaggatgt agccttgtta
     2101 tacttttgcg gattcgccac tagatctaac gcctttttca caagttcttc ccgttctgcc
     2161 tttgctttca ctgcatactt ctttgaccag aaaacgactt gcttttcgta gacagttttt
     2221 tttgcttttt ttccactttt catcgttaca ttaatctctc gcgcaatacg tctgctcttg
     2281 attttgaatt cggcatcttc accagctggt ttgccgttag tgccagcata cccttcatta
     2341 tcaagtacat aatttttaaa ttcgtttgta gcaccgcgga cagaaaagct gaacacatat
     2401 ccatttctgt tcttcccgcc ggtcaggtag taaatattgt cacctgtgat aatccccatg
     2461 tcagcgacta ccacaattct gcccgtgtcg taatttttac gaacctcacc gatgacggag
     2521 cgaaacgttt ccttatccag cttgtttccg ggaaaaagct catagtgcag tggtatcccg
     2581 tttgcatcca ttgccaggcc catttgtatg atcggattgg ggcgcttctc tttggatggg
     2641 ccgaattttc gtacttcatc gggtttgtcg atctcgaaat aaaaatttgt gacatcataa
     2701 taaaccgttt ttgtgtcacg cccgtatttc tctgtgatct gctcatttag aaattgctga
     2761 agatctttgg caatcttggc atagtgggaa agggcacgat agacatcagg cagcgaaaag
     2821 ttgaagcgct cgaagtaacg gcctttctct tcgaaggtct ttttcttgga gccgggggaa
     2881 agtaacctag agatgacaag cagcatcata atggagtttg tattgaactt gaaatcttca
     2941 ttgcgtgctc tgttatttaa aaaacggtgc agcccgagtt catgataaat cttcaggatt
     3001 gcggcatatc cgaggttttt cctgtcatct gttccctgca caagctgttc atccatgttg
     3061 aaggttagtg ttaacttttt cttattgttt tcttcatccg tcattttacg ggcaacttct
     3121 ttgaaatggg cgatggggtc ttggtattcc ttttctagct catctaagta gccgatagat
     3181 tgaattgttc tagcttttgg gttgccagtc accttatcac gataactttt ttcgatggaa
     3241 agataaactc taccgttttt tctacgtgat tgtttcaggt tcaaattagt cacctccgcc
     3301 tcgaattaca tagttatatt ataccatata aatctataca ttcctatacc tttttatgat
     3361 taaaattaaa aaaaataata cccatgcagc attgtgccac aagggtttct ggcttttcgt
     3421 ttctagttaa gctgtcaaac taacgaggct aaaatattat ttctaagtag aagttatagt
     3481 gcgtatgtgt ttcctgtttt attcccatca tgcaacagct aggtaatgtt tgctagccgc
     3541 ggataatagt ggtataataa acaggtggag ggatgaaagt ggacaactgt attttttgta
     3601 aaatagttag tggtaaagca ccggcagata ttgtttatga aaatgaagac atgatagcct
     3661 ttcgcgatat aaatccacaa gctccaaccc atgttttggt agtgaccaag caacatttcc
     3721 aataccttga tgaacttcct acacatgatc tgcagctggc aggtaaaata ttgttaaccg
     3781 tcaaagaagt tgcacagaag ttaggtgtaa caggcggtta taaagttgtc acaaactgtg
     3841 gagaaccggc tgggcaaata gtgccgcatc tacattttca tcttgtggca gggaaaaagt
     3901 ttcttcccta aagacaatta gctacttgtc cgtaaagtcg ttgacagact tcgctgaaaa
     3961 cgatataatc aaaaaagtat gcgggtagat tatactctct ttcgtcccgg tattttccca
     4021 tgcaggttgg tagtgagggg agggaaagta gttgacacag atcaaggttg gtaaaaatga
     4081 gactcttgat agcgcgattc gtcgctttaa aaaacaatgt gcaaaaacag gtatcctcgc
     4141 ggaggtacgt aagcgcgagc actatgagaa gcctagtgta aagcgtaaaa agaaatctga
     4201 agccgctcgt aaacataata aaaagcggtt tggttaagta tgtgaacagc aggtttatta
     4261 aaacctgctg ttttaattta catagggata gtatataatg aaattgcaaa tagctaagga
     4321 ggtagagcca tgtggagaag aagagttttg gcgctccttt tattgttaat gtttttaatt
     4381 gtgcccctgg ggggcctgca ggctgctgag acagccgaaa aggtttacgt gattaagtta
     4441 gaaggtgaag tagaggccgg actttatcat tttttgcagc gtgcctttac agaagcggca
     4501 gataacggcg cttcggctat tattatggag ttaaatacac cgggtggact ggtaaactca
     4561 gccaaggata ttcgtgactt aatttatgac agcggtgtac cagtttatgc ttatgtgcgt
     4621 catagcgcct tatcggcagg ggcttttctg gcactttcct gccgcgagct ttatatggca
     4681 ccgggtagta ccattggtgc agcgcaaatc caaaacctaa gcggcgaagc agtggatgag
     4741 aaaataattt ccgcctggga agccgagatg cgtactgtgg cgcagaacca aagcaaagat
     4801 cctcaagtcg ctgccgcgat ggtacgacaa gaaattgcta ttgaggggtt agtcagtgac
     4861 aagcaattgt tgaccttaac ttcggttgaa gcagaagaaa ttggttttac ggatggtatt
     4921 tttgccacca gagcagagtt attgacggcc ttaggtttgc caggtgcagc tatttacacc
     4981 gtgaaggtca gtccggcaga gagcctagcc cgttttgtgg ccaaacctgt tggcgctacc
     5041 cttttgctga ccattggttt ggcggccttg gtgattgaga tcttcatggc gggctttggt
     5101 gcagcaggtg ttgtcagcat tttggctttt gccctctatt ttggcggcag tatcattgca
     5161 ggttttgctg gcaatgaagt tatcattctt ttcatcgcgg gcattatcct gatgctgatt
     5221 gaagctgtaa ttcctaattt cggggtagtt ggcatctcag ggattgtggc tatgattacc
     5281 gccatcatct tatctgcctc caccgccgag cagggagcgc gcattttcct tacatcgtta
     5341 ttattaacag ctgctgtcgt ggcggtagct ttccgttact taaaacgttc taaactttgg
     5401 tcgcagatta ttttgcagta tgcagaaaca aaagaacgcg gttatgttgg ccctactgat
     5461 tatagctctt tggtcggtaa aacagggcaa acactaaccc cgttacgccc agtgggagcg
     5521 gtggagatag atggaaaacg tattgatgtg gtgagcgagg gcaattttat tgccaaatca
     5581 tcagcagtaa tagtagtgaa agttgaggga actagggtga ttgtacgccc acaataaata
     5641 tatgtaggag gtaaggaaat gccagaactt ttgataacat taggtttaat tgttcttggt
     5701 ttggctctct tgtttagctt tgtgccggta ggcttatgga tttcggcgct ggctgctgga
     5761 gtgaagattg gaatttttac acttgtcgct atgcgtttac ggcgtgtgcc gccggctaaa
     5821 atcattaatg ccctaattcg ctctagcaaa gcgggattag atttaaaggg agaccagctg
     5881 gagagccatt tcctggctgg tggtaatgtg gatcgggtca cgaatgcttt aattgcagca
     5941 cagcgggcgg gtattgagct cggttttgag cgtgctgcag ccattgattt ggcaggccgg
     6001 gatgtgctgg aagcagtaca ggttagtgtt aacccgcggg tgattgaaac ccctactgtc
     6061 gcggcggtag ccaaggatgg tattgaagtt aaagctaaag ctcgggtgac tgtacgcgct
     6121 aatattgagc gattaatcgg tggtgcaggt gaagaaacta ttattgcccg ggtaggtgaa
     6181 ggtattgtta cgactattgg ttcttctgaa acccataaag cagtattgga gaaccccgac
     6241 tctatctcac aaacagttct aaataaaggg ttggatgcag gcacagcttt tgagatactt
     6301 tccattgata ttgccgatgt tgatgtagga aaaaacattg gcgcccaact gcaaacagac
     6361 caggcagaag ctgataaacg gattgctcag gccaaagctg aagaaagaag agcgatggct
     6421 gtagcacatg aacaggaaat gaaagcaaat gtgcaagaaa tgcgtgccaa agtggtggaa
     6481 gcagaagctg aagtgccccg tgcctttgct caggccttac gtgaaggtaa gttagggatt
     6541 atggattatt ataacctgca aaatattgaa gcagatacac aaatgcgtga ctccattgct
     6601 aaactaggcg gcgggagtga caaggatgat aacaaaaaca aaaagtaagc tgtgggaggt
     6661 aagggtatgg aaaaaatagc acttttgctc atctttttgg ttttttcagc agtgcgtaca
     6721 ctagccgaaa accaagccaa aaaacctcag cagaaaacaa ctattccctc ctcgagaaac
     6781 cgtcccagcc gaccttttga ccagccgtta cgtaaactgg aacaagcact tagggaagct
     6841 gaagctgttt tgtccgctaa acaagaacgg ccgcagcggg cacaggctaa acgggaaact
     6901 cccaatgaca tgtattctgg agaaggtgtt tctttagagc atccttcacc cgctccccga
     6961 gaaatgggca gtctaaatgt cgctcctcct ttacaaagca cggtgctacc agtagaaaca
     7021 cttatgctgc aagagggctt tatgggcgac aaggaggaag tgttgcgcgg tatggtctac
     7081 gccgaaattc tcggcccccc caaagccttt cgacatttga aacgcccgag ctggagcaaa
     7141 tagagcccgc acaataaaaa aagcatagcc tgtaatcaag agcgaggaat caatcctcgc
     7201 tcttttatgt aacctcctaa cgcagatagc tgggctcaat gcgcaaggta attgttaagg
     7261 aatgttaata aattattaac atctagcagg attatgagat gatgtgccga atatttatag
     7321 ataacaagtg ttaaggttta ttaacaattg ttaataaata gctaggctgg atgattacag
     7381 caaggaggat acatcatgtc acgtggggga gaagtgctta agctctgtaa agagagaaga
     7441 ttcattgttc ctatacttac tttatgtgtg gtcatactga tggtggctac cgtttatagt
     7501 tatcatgctg gttttggtta tgtcgtggtc atcgacggag aagaggcagg atttttgtct
     7561 aaggctgatt taggggaact acccgtattt attgctcagc tcactgatga ggcacaagct
     7621 gcctttcaaa tgcaagtcct gttgaatgaa aatttagatt ttgtcgaagc taggcggccg
     7681 ggagaacatc aaagtactgc tgttgtcaaa gatgaagtgc ggcagagact ttcttttagt
     7741 acgtatggct ttgttctcac cattgatggc aaggagacag tagctctacg cagtgaagat
     7801 gcttatgata aagtgctgcg catcatgaag caagcttatc tcgcagcaaa ggaagatatt
     7861 attttaaaag actatgccat tcaggaagaa atagacttta ttaagcgtcc agttcaacct
     7921 gatgaaattg tgacggtaca ggaggcagct aatatcttac ttaccgggct gccgcggaac
     7981 cagactcatc ttgtggccag gggtgattct ttttggtcga tttcccggga gcataatatt
     8041 agtgttgctg ccctggcaga ggctaacccg caaatcaaaa atcagcaaat aaaaccgaat
     8101 gacgaactaa acctgattac cgtagaaccc ttggttaatg tgttggttgt ggaagaaata
     8161 acagtgactg agaaaatccc ctttcagacg caatatctta aagacaagaa aatgtatacg
     8221 tcacaaacaa aagtaaaaac gaacggtcaa atgggtgaga aagaaataac atatctagta
     8281 acgttggcaa atgatgagga agtagaacgt acaatcgtca aagaaactgt cctgagtgaa
     8341 ccagtggaca aagtggtggc aaaaggaaca gcgaccgcac ccaaatccgg tacaggtcgt
     8401 tttatgtggc ctttggcgaa cggcggcacc attacttcta cttttggatc acgttggggt
     8461 aagatgcatt atgctgtgga cattgccgca gcaggtggta cccctattcg tgctgctgac
     8521 ggtggtgtcg tgaaaacatc tagctataat ggcagctatg gcaatctggt agtaattgat
     8581 catggtaacg gttatagtac ctattacgcg cataattccc gcctcatggt aggggtaggg
     8641 gagcgcgttg ctaaagggca aacagtgtcg ttaatgggca gtacaggcaa cagcaccggc
     8701 aaccatgtgc attttgaaat acgaaaaaat ggtactccga ttaaccccct taaattcttc
     8761 tagcttgctg tgttaatagc taacggctac cgtcaaggtg gccgttttct ttgcttgcat
     8821 aacaggtgaa atgtatgcat agttatgata gcaggacagg gggggcgcgc gtgggcaatt
     8881 caaagctaac taaatggcgt gaccgtttgg tagatgccat ggagttacct aaagaaatca
     8941 tccttaattt acccaaagtc accattatcg gtaatgttca atgctatatt gagaaccacc
     9001 gtggtgtgat agagtattct agtgagcgtg tccgcgtggc catcaatagc ggtgaattaa
     9061 ttgtcagcgg gcaagacctc tttatcagat atatggctaa tgaagaaatt gccgtggatg
     9121 gtagtatcaa tgccgtgcag tatgagttgt agggctgagg gggttggcgt gtgtttatcc
     9181 taaaactacg ctactttatc acaggttatt taatagttgc cgtgcaaggt cggaatacgg
     9241 aaaagtttat taacttggca atccgtcaac atatcgcctt atgggatgtg caccgcagcc
     9301 ctgaaggtgc ttcactgaag gtagatcttg atagcttttt tgagctgcgc cacttggcta
     9361 aacaaacggg ctgtcgtgtg cagatcatac gcaaagcagg attacccttt tttttcaatc
     9421 gtctaactag acggcgaggt ttgctggcag ggctttgtct gttttttata gccctttatg
     9481 ttctttcttc cttcattttg tttattgatg tacaaggcaa tgaaaatcta gagactgcct
     9541 atattcggga attagcagcg caagctggtg ttaaacctgg tctctttaaa gagcgtttag
     9601 caaagaatca ggttacttcc caaatgctga ttgcagaacc aaagttagaa tgggttggtc
     9661 tgcagataca aggtacaagg ttagtgatag aagtggtaga acaaataaag ccgccggtgg
     9721 aaatcgatac acatgcgcat ttagtggcgg caaaagatgg tttagtgaca gatgtgctcg
     9781 tcatagttgg ggaagctaat gttgaagtgg gagatactgt gaggcgtggt cagctgctga
     9841 ttgcagggta tttgtctcct gaaccacagt atgtcccaga agaagagcaa gcaatagtac
     9901 gcatacctgt gcgggcacgc ggcgaagtaa cggcacgtgt ctggtatgaa ggttatggtg
     9961 aagcagaatt gactgaggtt attcgtaccc gtacaggcag ccacatgagt ggttggagct
    10021 ttttagtgga tggccagtca atcttacgcc tcggtcgtaa caatattcca tatcaccatt
    10081 atgatactga aaccacaagc aaacttctat tagaaaggat tattagcatt cctgtcgaaa
    10141 ttgtaactga aacgcactat gaagtagtgc tgcaagagaa gcagctaacc tttgaacagg
    10201 gcgtagagct tgcgactgaa cgtgccagaa agttggctga actgcagctg ccgccgggtg
    10261 agctcgtgga gaatgttaca gttaaagaag tcgagttaaa caaagaaggt tttgtagggg
    10321 tacaatatat tttggaaaca agtgaaaata tcgcagaaga agagatagta aacgggggcg
    10381 aagcaacgtc ttgagatata atgagaacag cataacaata cctgtagaca gtaatgagga
    10441 aattaagcaa cttttaggcc gtttcgatca acatgttact ttgttggacc ggtatttcaa
    10501 ggtaagaata attccgcagg gtaatgctat tattatcgaa ggaaaacagg atgaagcgga
    10561 aaaggtagct ttactattta gagaactacg ctggctgtta cagcaaggac aagttcctac
    10621 gctgcctgat gtagaatatg ccattgaatt gattcgcaca ggacaagctg aacgcttacg
    10681 cgggttgttt ggagacacag tctttcttac gccccgaggt aaaaggatca aaccaaagac
    10741 tattggtcaa aaagagtatt tagatgccat ccgaaaaaga gatatcgtct ttggtatcgg
    10801 tccggcaggt accgggaaaa cctacttggc gatgaccatg gcagtaaaag ccctgaaaga
    10861 acgctctgta caacgtttag ttctcacacg ccctgcggtg gaagcaggag aaaatctggg
    10921 ttttttaccg ggagacttgc aggataaagt tgatccttac ctgcgtcccc tgtacgatgc
    10981 tttatatgat ctgcttggta gtgatgtcta tcatgcttat ttagaaaaag ggatgataga
    11041 aattgccccg ttggcctata tgcgtggccg tacacttgac gatgccttta ttattcttga
    11101 tgaggcacaa aatacgacgg ttgaacagat gaaaatgttt ttaacccgtc tgggttatgg
    11161 ttctcgagcc gtgattactg gtgatattac gcaaattgat ttaccgcggg agcgtttttc
    11221 tggcttgaga caagtgcagt ctattttaaa ggatattccc ggcattgatt ttatttattt
    11281 aacaaaaaat gatgtggtac ggcatcctct agtgcagaaa ataataaatg cctatgaact
    11341 gcatgaccag gagcaggagc ggagtgatta acagtgggtt ttaccagtcg cgtcaaggag
    11401 cagttaaaac aaaatagttt tctatctcat caaccacgac gccaacggat gctgttggta
    11461 attttgcttt atcttgcttt gttattcgtt cttttcttta ctttaaaatc aaacaagctg
    11521 gacttggaat taggtaagcc tagtccgcga cgtattgtgg ctgagtggga agcgctggat
    11581 ggttatgaaa cggaacgatt acgagaggaa gcggctcaga cagtcgccga atcttttgat
    11641 tatgatccgg tagtcttaag ccgcgcggaa gagcgcgcta ttaccttttt taatcaagtc
    11701 caagaagtgc gtgagctgga actagaggac ggagaaaaga tggcccaact aatagcagca
    11761 gttgaagtgg agctcactga accgctagcc cttgttttgc tgcaggcgcg tcagcaggat
    11821 ctgcttgaca tgcagtcaca agtgagagaa gttttgcagg ttatcctgca gcagggtgta
    11881 aaaccagccg gtgtagaaac agcgcagcgt caagcatccc aagaaataaa ctttctgatt
    11941 tttgatcaag atcagaaacg tattatggtg cgcattgctg aggcggtaat tgagccaaat
    12001 atgatttata atgccacaac caccaatcaa gctcgtgaaa cggcccgtga agctgtgcaa
    12061 gcggtgcgta ttctcgaagg tacagagatc atccaagagc gtgaaacagt tactcaacgc
    12121 catttagagc agcttgaagc actggggctt ttacgtaata gatccggcgg cattcccatg
    12181 ttttttggtt tagcagctat tctgctgatt atctttatca ccgttagcta ttatctgtat
    12241 atttaccaga aagcgattta tcatgatata gtacgtctat tattgttagg gttaataatt
    12301 ttacttactt taacctttac catcgcggct aattatttct ctggctattt agtgccagtg
    12361 gcgatgggtg catttttgat tgccgtgctg cttaatgcgg agcttgctgt gctgctgaca
    12421 ctggcttttt ccgtatttgt ggtcctgata acaggtaatg attttcgtta tatgctggtg
    12481 gccatgtttg gtggtttgat agcatgtttc agtgtgaccg atttaagccg tcgttcagat
    12541 ttgactaaag caggtttata tgtatctgct gttaatgtag ttgctgtctt tggtcttttt
    12601 ctttatacgg gcaatctgcg ttttggttat gacatcttac gcgaattcgg ggttagtatt
    12661 tttgcgggta tcgctaacgg tctcttttct gctgtgatgg cgattggtct tttgccttat
    12721 ttagagagtg gctttggtct tactactgca gttaccttat tagaattagc caatcctagc
    12781 catccgttgt taaagcggct gctgatggaa gctcctggta cctaccacca tagcttatta
    12841 gtggctaacc tggctgaggc agcagctgaa acagtaggcg gtgatccctt gctggcacgg
    12901 gttggtgcct tttatcatga tattggcaag ttgaagcgcc cctacttttt cattgaaaat
    12961 cagatggggg gagcaaaccc gcatgacaaa atttctccta atctaagtac gttaattatc
    13021 acttcccata ttaaagatgg cctggagatg gcgcaaaaag caaacttgcc gcaagtaatt
    13081 caggatatta tctaccagca ccacggtact agtttagttt ctttcttttt tcaacaggcg
    13141 gaaagaaatc aggatagcaa agagattatt cgtgaggaaa actttcgtta cgaagccgca
    13201 attccacaga ctaaagaggc agctattatt atgattgccg atgccgtgga agcggcggtg
    13261 cgttcacttt ctaaaccagc agttgggcgc gtggaagcga ttgtgcgcaa gatcattaaa
    13321 gacaaattaa atgatggtca atttgatcaa tgtgacctta ctttaaaaga tttagatact
    13381 attgcggtca ctttggtgaa gatactctcg ggaatttatc atacgagaat tgagtatcct
    13441 gaaaaagagt taaaggcaga aattgaaagg ggccgaaaaa atggcggttc tgttaaataa
    13501 tgagttaaag gaaaaactta aatcaactat gctcacggcc atggagtcaa ttagcgcctt
    13561 tgctctggca gagcataact tacccaagac aacggaaatt agcctgacaa tctgtgacaa
    13621 tcatacgata cagctgatta ataagcagtg gcgtgatgta gatacagcga ctgatgtact
    13681 ctccttttcc cttctaacac ctggagaaga cttggtgata gctggtgagc tgctgctggg
    13741 ggatattatt atttccctcg agcgggccag agagcaggcg gcagagttta accatagtgt
    13801 ggagcgtgaa atattatacc ttttcacaca tggcctttta catttgttag gctatgatca
    13861 tgaagagcct gcagcacaac aagctatgcg tctggccgaa gaaaaactgc tgctggccgt
    13921 aggagcgaca cgggatgaac tttaaacaca aaagttttaa ggaaagtatt tactgtgctc
    13981 tttgtgggtt agcggatgcc tggcgcagtg aacgtaatat gcgctttcac accgcggcgg
    14041 cagtggcagc ggtggcagta ggcgggtggt tccgcttgac gccgatggaa tggctttttt
    14101 tattttctgc cgtatttctg gtgatgacca tggagatgtt aaacacttcc ttggaacgag
    14161 tggttgattt gttcacgcgt gagtatcatc ccttggccag gttagctaaa aatgccgccg
    14221 cgggggctgc cttgctggca gctatttatg cgatcatcgt cggtttttta gtctttggcc
    14281 cttatgttgc agagagcttt aatctttttt aatagtttca taaagacttt ttaattactt
    14341 tctgataaaa ttagagttaa tgtaagaatt tgggtgaaag gaggatgagc tcgtaaatga
    14401 gtaattctga tactgctttt tcgatgagca aaaaaggact aatactagtt ttaatcgtct
    14461 tatttataaa tacagtgttc ttagc
//
LOCUS       DASRMS010000176         2044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_251290, whole genome shotgun sequence.
ACCESSION   DASRMS010000176 DASRMS010000000
VERSION     DASRMS010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251290"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..512)
                     /locus_tag="VFC74_07715"
     CDS             complement(<1..512)
                     /locus_tag="VFC74_07715"
                     /EC_number="1.4.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047850 - diaminopimelate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate dehydrogenase"
                     /protein_id="HZK25258.1"
                     /translation="MNKKIRAAIVGYGNIGRYVIDTIVHTTDVEIAGVVRRNVDKIPA
                     ELEAYKVTNSIDKLKDVDVAILCLPSRLTEGAAKEILAKGINTVDSFDIHQDIVALRA
                     SLDEVARRHGAVAVVSSGWDPGSNSVVRALMQAMAPSGITYTNFGPGMSMGHSVAVKA
                     IEGVRDALSIT"
     gene            819..1583
                     /locus_tag="VFC74_07720"
     CDS             819..1583
                     /locus_tag="VFC74_07720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008679938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HZK25259.1"
                     /translation="MKLKGKVAIVTGASSGIGRSIARLFASHGAIVYAVARREERLKA
                     LQEETKDCVGKIIPHTADLRNKDEAEYIITLACEEQGKLDILVNNAGVMDDFSPIGEV
                     SDEMMQNLTDINVMAPFYSTRKAVNCFLKQGGGNIINVSSIGGLHGARAGAAYTMSKH
                     AIIGLTKNTGFMYARKNIRCNAICPGGVETEVGEGEYMKQMNQTGIDTIMPSTCGNPR
                     NGKSGEIATIALFLATPESSFINGQSIVADSGWTAF"
     gene            complement(1580..2035)
                     /locus_tag="VFC74_07725"
     CDS             complement(1580..2035)
                     /locus_tag="VFC74_07725"
                     /inference="COORDINATES: protein motif:HMM:NF015563.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HZK25260.1"
                     /translation="MLTAALVIALAGGIIQILSDGKIMDPILNSLAQVMESAGKTTTV
                     GAMFVMQSIINIFIPSGSAKAALTMPIMAPFSDVIGLSRQATVMAYQFGDGVTNMITP
                     TSAVLIGALGIARVPYEVWFKWFWKTLVVLFVLAFILLLPTVFMQLDGF"
BASE COUNT          612 a          490 c          437 g          505 t
ORIGIN      
        1 gtgatagaca aagcatccct caccccctcg atagccttta cagccacgga gtgacccatg
       61 ctcatgccag gaccaaaatt ggtgtatgta atgccactag gcgccattgc ctgcatcagc
      121 gccctcacca cagagttgct tccggggtcc caccccgaag ataccacagc tacagcaccg
      181 tgcctgcgag ccacctcatc aagcgatgcc cgtagcgcca caatatcttg atgaatatca
      241 aaactatcga ccgtgttgat gccttttgcc aatatctcct ttgcagcacc ctccgtcaat
      301 cggctgggca agcagagtat agccacatcc acatccttca atttgtctat cgagtttgtc
      361 accttgtaag cctctagctc ggcaggtatt ttgtcaacat tgcgacgcac cacaccagct
      421 atctccacat ccgttgtgtg cactattgtg tctatcacat atctgcctat attgccgtag
      481 cccactatgg ctgctcttat ctttttattc ataattaatc gctttttttg atagatacaa
      541 aaataatcaa aaatattgga ctttatttta tttaattgat aaaaaggtga tgcgtgaaca
      601 gtgaacagtg aacagtaaat agtcactagt agccgctatg aaccaaactc cgtaacccgt
      661 aacacacaac ccgcaacccg taacccgcac cactgataac caacctatta ataatcaata
      721 accaacaatt gatatttaac tacatttgtt tgcattttgc catatatata gctacattta
      781 tagacaattg ttctaaattt aatccaaaag aaggcattat gaaactaaaa ggtaaggtag
      841 caatcgttac aggagcaagt tcaggtattg gcaggtctat tgcacgtctc ttcgcttcgc
      901 atggagcaat agtgtatgca gtggctcgca gagaagagcg actaaaagct ttgcaggagg
      961 agaccaaaga ttgtgtagga aaaattattc cacacacagc cgacctcaga aacaaagacg
     1021 aggcagagta tatcatcaca ttggcatgcg aggaacaagg caagctggat attttggtaa
     1081 acaatgcggg tgttatggac gattttagcc ccattggcga ggtatctgac gagatgatgc
     1141 aaaacctcac cgacattaac gtaatggcgc ccttctacag cacgcgcaag gctgtaaact
     1201 gtttcttgaa acaaggtgga ggcaacatta tcaatgtaag ttctattggc ggattgcacg
     1261 gtgcaagggc tggtgcagca tacactatga gcaagcatgc cattattggg ttgaccaaaa
     1321 acacgggttt tatgtatgct cgcaaaaaca ttcgatgcaa cgccatctgc cccggtggtg
     1381 tggaaacaga ggtgggcgaa ggtgaataca tgaaacagat gaatcaaacg ggaatagaca
     1441 ccattatgcc tagcacgtgt ggcaatccta gaaatggcaa aagtggcgaa attgctacta
     1501 ttgcgctgtt tttggctaca ccagagtcta gctttattaa tgggcaaagc attgtggcgg
     1561 atagtgggtg gacggctttt taaaacccgt ctaactgcat aaaaactgtg gggaggagca
     1621 aaataaatgc caacacaaat agcactacca gcgtcttcca aaaccactta aaccacacct
     1681 cgtaaggcac cctagcaatg cccagtgcgc caattagcac agcagaggtg ggtgttatca
     1741 tattggtgac gccatctcca aactgatatg ccatcacggt ggcttgacgc gacagcccaa
     1801 taacatccga gaagggtgcc attataggca tcgtaagtgc cgcttttgcc gagcccgagg
     1861 ggataaaaat attgataata gattgcatta caaacatggc accaacggtg gtggttttgc
     1921 ctgcactctc catcacctgc gccaatgaat taagaatagg gtccataatt tttccatcag
     1981 ataaaatctg aataattcca cccgccagag caattaccaa tgccgctgtg agcatatctc
     2041 accc
//
LOCUS       DASRMS010000177        32974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_186091, whole genome shotgun sequence.
ACCESSION   DASRMS010000177 DASRMS010000000
VERSION     DASRMS010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 32974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32974
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_186091"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(212..2986)
                     /gene="ileS"
                     /locus_tag="VFC74_07730"
     CDS             complement(212..2986)
                     /gene="ileS"
                     /locus_tag="VFC74_07730"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004624263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HZK25261.1"
                     /translation="MEEKDYAKTLNLPKTDFPMRAGLPKREPEQLQKWQEMDIYGQVK
                     AHRQGRPKYILHDGPPYANGNIHLGTAMNKVLKDIIVKNRTMLGYDAPYVPGWDAHGL
                     PIEHAVLKSGKVKRNEVSSLELRQHCREFALRFLDLQREQFKRLGVRGDWENPYITLQ
                     KEFEAKEIEVFGAMAEKGYIYKGLKPVHWCPQCETALAEAEIEYQDHRSPSIYVRFPI
                     ADAKGKLAEAENTWFVIWTTTPWTIPANLAICLHPEYTYVRVSTPEHGDLILAEGLLA
                     EALQACEITDYTIKEKYLGKDLEGMLCQHPLYERTSLVVLGDHVTLEAGTGCVHTAPG
                     HGQDDYEVGLRYGLDIFAPMDHKGHFTAEAGQFAGLHYDAGNKAVTQALADQGALLKL
                     SFIKHPYPHCWRCKEPVLFRATEQWFASVDGFRKETLAAIKDVKWIPAWGEERIHNMV
                     VDRHDWCVSRQRVWGVPIPIFYCTACNKELINEDTIRKVAEIFAQEGSDAWFAREASE
                     LLPEGTVCPACGHHEFRKETDIMDVWFDSGSTHAAVLETHPDLVSPADLYLEGSDQYR
                     GWFQSSLLTSVATRGRAPYKATLTHGWVVDGEGRKMSKSVGNVVAPEQIIKQYGADIL
                     RLWVSSSDFKSDVRVSPDILKQLSEVYRKIRNTMRYMLGNCADFNPASDAVAYENLDE
                     LDRYALHRLQVLVERISRAYDDFDFHVVFHAIHNFCVVEMSNFYLDVLKDRLYTSVAN
                     SQARRSAQTVMYEILLTLVKLIAPVLTFTAEEAWNYLPVKDVGSVQLADWPQVNVAYK
                     NETLATRWQALLTYRDVVTRRLEEARKEKRLGSSLTAALELYPDQAAYAALQPFKERL
                     AELFIVSDCTLHEPGSGTAETIVEQGLGVAVKTAAGEKCERCWMIHPQLGTDPSHPTL
                     CPRCTESVNA"
     misc_binding    complement(3053..3267)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(3446..3931)
                     /locus_tag="VFC74_07735"
     CDS             complement(3446..3931)
                     /locus_tag="VFC74_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HZK25262.1"
                     /translation="MPLTPIDIQNKEFSRSIRGLAANEVDEFLNRVAKDYEELIKDNI
                     TAKEQISQHKEKLAHYQKLEHTLHNAIVVAQETAEEVKRNASKEADLIRREAEKDAAR
                     LLEEARYKASRILSEHDEVYKQAQIYKMRFRSLVEAQLAALEMEDWLSPQAEHGVQQG
                     A"
     gene            complement(4086..4874)
                     /locus_tag="VFC74_07740"
     CDS             complement(4086..4874)
                     /locus_tag="VFC74_07740"
                     /inference="COORDINATES: protein motif:HMM:NF037631.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmH/Sll1252 family protein"
                     /protein_id="HZK25263.1"
                     /translation="MEREKILKQFEQEIEKITACTVLDKLAQVQQTGKPQTTDFLDPF
                     QQRVADRVLHHFKDIKQVVWGGYEAAERARLMVFSAVKQATVNDVPLAFLAANAPAQA
                     SGLSHRDWLGALLGLGLRREKVGDIIVTSETSAQVVVHPELLNFLLANWQQIAKYNLE
                     LCEITAAELSPTVGRVREIKTTVASLRLDAVASCGFGLSRSKLTPAIRAGHVKLNWQS
                     VKSASAAIKEGDVIAVSGRGRVEVAQVLGQSKKGRIQLLLKKHL"
     gene            complement(4924..5352)
                     /locus_tag="VFC74_07745"
     CDS             complement(4924..5352)
                     /locus_tag="VFC74_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HZK25264.1"
                     /translation="MASIWEKTLTFFGLMEEAEEEETYEEQDNPRQSSARKGTVLNLH
                     TAKNMRLVITKPYEFAEAQAIVDHIKNRKPVLVNMEETDKGEAKRIVDFICGATYALE
                     GNMQKVSQQIFVFAPAQVEVNAEMRRELSNRGVVTDLDKE"
     gene            complement(5369..6040)
                     /locus_tag="VFC74_07750"
     CDS             complement(5369..6040)
                     /locus_tag="VFC74_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HZK25265.1"
                     /translation="MSDLAKQIAKVQQRLQQAARRSKNKPVQLLAVTKKVPSECIAQA
                     FSLGLKTFGESRVQEALPKLAHLPQAEWHYIGRLQTNKVKDVVGNFTLIHSLDRWSLA
                     EALQREAAKREQRVRTLVQVNIGGEAQKGGLEPYEVAEFIHELSTFSHLQVEGLMAVP
                     PYSEDPEAARPYFRSMYQLFTKLQIPGVHMKTLSMGMSNDFEIAVAEGANLVRIGSLL
                     FGERA"
     gene            complement(6037..7002)
                     /locus_tag="VFC74_07755"
     CDS             complement(6037..7002)
                     /locus_tag="VFC74_07755"
                     /inference="COORDINATES: protein motif:HMM:NF024112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HZK25266.1"
                     /translation="MRKRRRFTVIEGQKTKSKRPKIQSLILLTLALLVVRIAWPFAHQ
                     ALAAIFVRTVNAQIGVLEHIVEAPGFIVRQEQVVTAPLTGTLKWLAADGERLAIGAPV
                     ATITTAGGTVQTVTMPAAGMVIKKIDGFEGALQPVILEQPAALDVAQLSEAKADLQEV
                     DDGAEVQQGSFIFKSINNYLWYYVAQFKHDAFAALEGKTTVDLRLSLDDSATVRAQIR
                     EVIEEEGTVTAVFALQEQLAGCYEERFAEVEIVTQRTRGIVLPTSALRVRGDETGVYV
                     LDQSLVRYRKVEVVTTEAQQVVVHGLPTGIPVIINPVFVKEGQKL"
     gene            complement(7010..7816)
                     /gene="pgeF"
                     /locus_tag="VFC74_07760"
     CDS             complement(7010..7816)
                     /gene="pgeF"
                     /locus_tag="VFC74_07760"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HZK25267.1"
                     /translation="MPKEKISPVTAIRYLQFENLQCAGLLHGVTLRSGGVSAPPYDSL
                     NMGLHVGDSEQDVRTNRQLAAEALTYQTSTVVVGEQVHGTAIARVTPEMAGCGHYQMT
                     DAVAATDGLICTEPEIVLMAHAADCTLLFFYDPVVRCIGLAHAGWRGAMAGMGPLMVE
                     ALSKLGSRPENLRVALAPTIGPCCYQVGENVAEQVPQIWRAEVLQPAAGAYYFDLPEF
                     QRLQLRAAGIKEENLVKSSYCTACHTDLFYSYRAAGGQTGRMAGVISMKW"
     gene            complement(7809..8345)
                     /gene="nrdG"
                     /locus_tag="VFC74_07765"
     CDS             complement(7809..8345)
                     /gene="nrdG"
                     /locus_tag="VFC74_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0051353 - positive regulation of
                     oxidoreductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HZK25268.1"
                     /translation="MKLNLAGLHQYSVVDGPGIRTVVFTQGCPHNCSGCHNPHTHNPA
                     EGQCVEISEVAAQILAETNLHGVTFSGGEPFLQARALASLAALLKARSPLLHLTVYSG
                     YTYEQLSQQAKKDVGTAMLLAASDLLIDGPYLEKQRDLFLLYRGSGNQRIIDVRASIQ
                     AGMLVLSELHYRGVQKHA"
     gene            complement(8371..10395)
                     /gene="nrdD"
                     /locus_tag="VFC74_07770"
     CDS             complement(8371..10395)
                     /gene="nrdD"
                     /locus_tag="VFC74_07770"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008998 - ribonucleoside-triphosphate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HZK25269.1"
                     /translation="MFTEIRKRDGRVVLFDAEKITDAIVKAAKAVGGDNRKIAENLTG
                     QVIKALEQLGYNGIIPAVEDVQDLVERVLIENGHARTAKAYILYRDRRNRIREGKSDL
                     MDTVKEILIETSRENANVSNSPSAKMLQIASAASKHYYLTNLLPEEYSQAHINGVFHI
                     HDLDYYGKTLNCLQIDLFKLLSSGFDTGYGYIRPPKRIASAVAQAAIILQSNQNDMFG
                     GQSFPHFDRSMSQIIATMPTTPDENEIFQAMEGLVYNLNTMHSRAGAQVPFSSLNIGT
                     ATTPMGRAVTKAILQVFEKGLGKGESPIFPNLVFRTKKGVNFNPEDPNYDLFVYALKV
                     AAKRLNPTFSFMDSTFNAKYGEEVAYMGCRTRVIANRHGDEVAAGRGNIAPVSLNLPR
                     LALQARDTNTFFVELDRLLRLAARQLLHRFEILGNLRAKDLPFLMGQKLYMGSEALGP
                     NDPIKEVIKHGTLSVGFIGLAETLQVLIGKHHGEDQEAQDLGLKIVAHMSKRVKQFSE
                     EFDLNFTLVATPAEGLSGRFIKIDREQFGIIPKVTDKEYYTNSVHVPVSYCISLFDKL
                     SIEGAYHNYCDAGHIAYVELEAPPGENYEAVEEIVRHMAASDVGYGGINYPIDECRTC
                     GFSGIMHDCCPSCGGTNVRRIRRITGYLSTEERFNSAKQAELHDRKAHLH"
     gene            complement(10417..10881)
                     /gene="nrdR"
                     /locus_tag="VFC74_07775"
     CDS             complement(10417..10881)
                     /gene="nrdR"
                     /locus_tag="VFC74_07775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HZK25270.1"
                     /translation="MKCPHCGCVESRVIDSRSTDEGSAIRRRRECTVCERRFTTYEKL
                     EETPLIVVKRNGNRQLFDRTKVLNGLIYAGGKQKIPLHVFEQLVDELERELRNSFSQE
                     VTSDEIGKRLLAKLYKIDQVAYVRFASVYHKFTNIDDFKKALAEMPPKNVTS"
     gene            complement(11036..11293)
                     /locus_tag="VFC74_07780"
     CDS             complement(11036..11293)
                     /locus_tag="VFC74_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlmC/YmxH family sporulation protein"
                     /protein_id="HZK25271.1"
                     /translation="MVKISDLRNRDVVNIVDGKRLGVICDLDLDLEHGRIAAIIVPGA
                     TRLFGFLGGGRDYIIPWENIVKFGVDTILVELIGPTSSSRY"
     gene            complement(11455..11652)
                     /locus_tag="VFC74_07785"
     CDS             complement(11455..11652)
                     /locus_tag="VFC74_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25272.1"
                     /translation="MHKRYAWQVGVSMLLVLTLFMVFLSPPGEAVQAFNQSNLIRVTE
                     VREVDGHYYYRRVAPVSEFWP"
     gene            complement(11676..12449)
                     /gene="sigG"
                     /locus_tag="VFC74_07790"
     CDS             complement(11676..12449)
                     /gene="sigG"
                     /locus_tag="VFC74_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigG"
                     /protein_id="HZK25273.1"
                     /translation="MINKVEICGVNTSKLPVLKNDEMRRLFTRYQAGDESARQKLVNG
                     NLRLVLSVIQRFNNRGEYVDDLFQVGCIGLIKAIDNFDLGQNVKFSTYAVPMIIGEIR
                     RYLRDNNPIRVSRSLRDIAYKVLQIRDSLANQYGREPNISEIAKELNLKREDIVFALD
                     AIQEPVSLFEPIYHDGGDPIFVMDQISDDKNQDEKWLEVIAIKEALQKLNEREKLILT
                     LRFYRGKTQMEVADEIGISQAQVSRLEKAALGHLRKHVQ"
     gene            complement(12570..13301)
                     /gene="sigE"
                     /locus_tag="VFC74_07795"
     CDS             complement(12570..13301)
                     /gene="sigE"
                     /locus_tag="VFC74_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011877061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sporulation sigma factor SigE"
                     /protein_id="HZK25274.1"
                     /translation="MRLLQHWKLRLRLAYLRFLHRLGVETLPEIYYVGSSETLPPPLT
                     QDEEVFLLEKLKLGDYAVKTVLIERNLRLVVYIARKFENTGIHIDDLVSIGTIGLIKA
                     VNTFDPHKNIKLATYASKCIENEILMFLRRNNKVKAEVSFDEPLNVDWDGNELLLSDV
                     LGTENDLVIKNIEAEVERKLLYTAIEKLSSREKRIMELRFGLLGKEEKTQKEVAVLLG
                     ISQSYISRLEKRIIKRLKKEIKKME"
     gene            complement(13298..14194)
                     /locus_tag="VFC74_07800"
     CDS             complement(13298..14194)
                     /locus_tag="VFC74_07800"
                     /inference="COORDINATES: protein motif:HMM:NF015384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E processing peptidase SpoIIGA"
                     /protein_id="HZK25275.1"
                     /translation="MYIEDLLALNFMMNASLLYLTARLTGREVTRTRVLAGGLVAALY
                     SLAIFLPASVIIFSWAGKIVASIVVILFTFKPQRPVELLRLCGAFFLISFFVAGTIFA
                     FYFFGSAPAMVKGGIFYIEPPRPGMLFSGVLVAFSLLMMVWHFSERQRKRKDFSFRLL
                     IHDAEQEVTVRALVDTGNQLRDPLSGKPLCVASYQALRPLLPQVLRTAYETGQDPVSA
                     LGLLNCEEPIRFGVVLYRSLENSGMLVTYRPPLVIVEHQNQREERSDLVFALTARPLS
                     LDNDTEVLLHPFILEVMGGVGC"
     gene            complement(14456..15505)
                     /gene="ftsZ"
                     /locus_tag="VFC74_07805"
     CDS             complement(14456..15505)
                     /gene="ftsZ"
                     /locus_tag="VFC74_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HZK25276.1"
                     /translation="MIEFDIEMEQFAQIKVIGVGGGGSNAVKRMIDAGLRGVEFVSVN
                     TDAQALYLAESGYKLQIGEKLTKGLGAGANPEVGKKAAEESREELKEALKGADMVFVT
                     AGMGGGTGTGAAPIIAEVSREIGALTVGVVTKPFTFEGKRRQAAAEKGIAELKEKVDT
                     LIIIPNDRLLQVVEKRTPMLEAFRIADDVLRQGVQGISDLIAVPGLINLDFADVKTIM
                     KETGAALMGIGVASGDNRTVEAAKAAIASPLLETSIDGARGVLLNITGSADLGLFEVN
                     EAADIVAEAADPDANIIFGAVIDESLQDEVRVTVIATGFDHRAGERKQIIDELTQRTF
                     NNDDIDIPAFLRRKR"
     gene            complement(15535..16758)
                     /gene="ftsA"
                     /locus_tag="VFC74_07810"
     CDS             complement(15535..16758)
                     /gene="ftsA"
                     /locus_tag="VFC74_07810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HZK25277.1"
                     /translation="MKRDLICGLDIGTSHVRAIVGEVNVDNSLSIIGVGTSPSEGLKK
                     GVIVDLDKTVEAIMHAMDEAERMVGTAIPSAFLGIVGSQVGLINNRGVVAVVGADKEI
                     TEEDVERVLQAAKVIALPPDREIIDVIPREFIVDGYDGIRDPVGMVGVRLEVDAMIIT
                     GMITSLRNLYRCVERAGVGIDGTALNALANAEVALTRDEKELGVVMVDIGGGTTEVAV
                     FQNGYLKNIAVLPVGGDHITNDIAVGLRTTLETAERIKLDHGAASAKVIADHATIELP
                     QISGKEARKVPAKELALIIEARLIEILQMAHEELLQLGYQEALPAGIVLTGGVSLMPG
                     IEKLAEQIFQTSARVAQPGYVGVKSPIYSTAVGIIHYVQHTQMFEVNEYRGGRKALPG
                     IFNKVKSWFMDMFES"
     gene            complement(16847..17647)
                     /locus_tag="VFC74_07815"
     CDS             complement(16847..17647)
                     /locus_tag="VFC74_07815"
                     /inference="COORDINATES: protein motif:HMM:NF015739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HZK25278.1"
                     /translation="MDKLQRKYRRKRQITSTADAVQFHKLKRYLLIVLIIFLTVWTSI
                     SFVKSDFFSVELFSFDGNNYTTEAEIRHAMQITEGENIWKLSLPLLEERIQAIPRVET
                     VSVTRRLPRTLNIKLQERKMLVLIPFQEYFLEIGWNGQILGTTQATQNYDLPLLTGVA
                     PIAGTVGQMLLTAEDLHQVQQLSEALKEEGVMISEINLEQADNLIIVTMDGLSVWLGV
                     DNFVEKANILLQITGQLNGKQAEGYLDLRAATAPVFHPLKDEKNKKNN"
     gene            complement(17704..18975)
                     /gene="murA"
                     /locus_tag="VFC74_07820"
     CDS             complement(17704..18975)
                     /gene="murA"
                     /locus_tag="VFC74_07820"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003542781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZK25279.1"
                     /translation="MEKFIVRGGKRLEGVVNIGGAKNSILPILAAVLLNKSGDEVFLT
                     NVPRIRDVIKMVEILRSLGVEITWSGTDMAIKTHNLTGFAIDEALMREMRSTIFLMGA
                     ILARFGQVCISYPGGCAIGQRPIDLHLKGLAALGAQLREQHGYIYASCSRLVGADIHL
                     DFPSVGATENIMLAAVYARGVTVISNAAKEPEIVDLQNILNKMGARINGAGTDHIRIE
                     GVTQLHGVEYAVIPDRIVAGTMMMAAAITGGNIILENVIPRHLDAVTAKLREAGVQIR
                     EDSGRLFVQAPQRLRALEYVRTLPYPGYPTDLQAPMMAVLTRAQGTSIIVENVFDSRF
                     KHVGELNRMGAQIIVASDNRTAIVHGTEHLMGATVTAPDLRAGAALVIAGLAAEGETV
                     VDGLEHIVRGYENLAGDLSKLGAEIKCITDG"
     gene            complement(18992..19885)
                     /gene="murB"
                     /locus_tag="VFC74_07825"
     CDS             complement(18992..19885)
                     /gene="murB"
                     /locus_tag="VFC74_07825"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HZK25280.1"
                     /translation="MEFVAEKIAKKVEMVKLSEPLSRHTSFKIGGPADLYVEPQNVEQ
                     LIFVLTCLQEQKKPYYFLGNGTNLLVHDDGYRGAVVRLAGQFREFSFTQELVSAGAAV
                     PLAVLAQKAEQLGLRGLAFAGGIPGSVGGALVMNAGAHGHSLGELLVDAQILNAALIL
                     HTCQPQELGLSYRQSNIRPPQVVCAVRLQLTAGDKDELVRQSREALAFRRNRQPRQPN
                     AGSIFKNPLGDAAGRLIEAAGLKGHRVGGAAISDVHANFIVNQQHATAQDVMTLISKV
                     QQEVSRQFGIELALEVRLLGY"
     gene            complement(20053..21153)
                     /gene="murG"
                     /locus_tag="VFC74_07830"
     CDS             complement(20053..21153)
                     /gene="murG"
                     /locus_tag="VFC74_07830"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:TIGR01133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HZK25281.1"
                     /translation="MKVIFTGGGTGGHIYPALALARYVRENEGAEVLFVGTEQGMEKQ
                     IVPAAGFELKTIPVIGLERRLSLRLGRAVYLAAAAVVTARRIIRSFRPDVVVGTGGYV
                     AGPVVLAARLAGIPTVIHEQNAIPGLTNVWLSRIVNRVCLSMPGSEKFFPRPQKTILT
                     GNPRATEAASAASGAPLQEPVLQPNLPVLLCVGGSHGALKLNEAFAGSLVRVLSETKA
                     QVVYVTGRRYYEELTAQLAPLQQQFPQRLHILPYHEQLPRLLAATNLMVSRAGATTLA
                     ELTALGVPCIVIPSPNVTNNHQEHNARMLSEHDAAVLIREQELTTAVLADQLVGLLSD
                     KERLQAMAEASKGLGIPDAAARMTAVMHEVCR"
     gene            complement(21167..22279)
                     /gene="spoVE"
                     /locus_tag="VFC74_07835"
     CDS             complement(21167..22279)
                     /gene="spoVE"
                     /locus_tag="VFC74_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein E"
                     /protein_id="HZK25282.1"
                     /translation="MAGRSDRKIRKKEADFILLFTTMTLLAIGIVMVFSASYYILRER
                     SDPYYHLRRQVQWAALGLAGMLFFSNYDYWKLKRWTNLVLLLSFIMLVAVFIPGIGEE
                     ILGARRWIRVGVLTFQPSDLAKVALIMFAAAYLSRRDIEVQKFTEGPLPILIIMGIFF
                     AMIMKQPDLGTGVAMAGSVMLVIFVAGMPLQQLALIGASAVPMLFYLIYSEPYRLQRL
                     MSFRDPWADPLDTGYQIIQSLYAIGPGGLFGVGLGQGRQKMHYLPEPHSDFIFAVISE
                     ELGFIGASTVLIIYFLLFWRGIKIALSAPDAYGSLLATGIISMIGLQTLMNIGVVTGS
                     IPVTGINLPLISSGGSSLLFTMCSLGVLLNISKYAK"
     gene            complement(22269..23651)
                     /gene="murD"
                     /locus_tag="VFC74_07840"
     CDS             complement(22269..23651)
                     /gene="murD"
                     /locus_tag="VFC74_07840"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HZK25283.1"
                     /translation="MDLLGKEVLVIGLARSGMASALLLLQDGAQVTVNDKRTAEDLTA
                     EEQLFLKENSTLRFVGGGHPANLVTEQTALIIKNPGVPLQLPPLQRAAELAVPVITEV
                     EHAAWHLTAPLVGITGTNGKTTTTALTGEMYRATGRQTFVAGNIGLPLSAVIPQVQAE
                     DVVVAELSSFQLEGTSHFRPHLAAILNITPDHLDHHGSWEAYREAKAMIFANQRAADA
                     VVLNADDPEALALRQRPHCQVYLFSRLREVERGAFVRDGLITLRDGQEEVTVCPQEDL
                     AIPGSHNLENALAATLLAWLGGVPAAIIAQVLTTFPGVEHRLEYVRTLAGVDYINDSK
                     GTNVDATLKALSAFPQRKVLIAGGYEKGADFAPFAQALAGEVSHVILLGQVAERLAAA
                     FREAGYFNFTFAASLAEAVQMAQAKAQRGEVVLLSPACASWGMFRDYEERGKLFKQAV
                     WQLRGQENGR"
     gene            complement(23739..24698)
                     /gene="mraY"
                     /locus_tag="VFC74_07845"
     CDS             complement(23739..24698)
                     /gene="mraY"
                     /locus_tag="VFC74_07845"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HZK25284.1"
                     /translation="MKQNLLITAVSAFLPGLVLAPFYIRQMRRLALGQQIREEGPRKH
                     LKKAGTPTMGGVIFLLVSLPATFLFAPPSTELYLVILLMLGNGLIGFIDDYLKVVRKQ
                     SLGLRARDKLLGQAITVVLFYLGWRTLGASTDVTIPFTTYSLELGSLYLPFILFFILG
                     FTNAVNLTDGVDGLAAGTAILSLLAYMLIAVLQQSLGLAGFAAAMIGAVFAFLAYNLH
                     PARVIMGDVGSLALGGVLAGLAILTKTELFLLIIGGVFVIETFSVIIQVLVFQSTGKR
                     VFRMSPLHHHYELGGYSEWNVVTGFWALAFLLAVLGLWLLGAL"
     gene            complement(24695..26086)
                     /gene="murF"
                     /locus_tag="VFC74_07850"
     CDS             complement(24695..26086)
                     /gene="murF"
                     /locus_tag="VFC74_07850"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HZK25285.1"
                     /translation="MEINCKTIAALVQGKIVTEANQEAEVKRVVIDSRGVQAGDFFVP
                     FAGEHTDGHRFIAQAADSGAVGCFVDQTAELTPPSELCAIAVPETLIALQQLAHGYRL
                     QYTLPIVAVTGSVGKTTTKDLIAAALSGRYQVLKTEGNLNNQIGLPIMLTRLTSKTEV
                     AVLEMGMSGLGEIAALAQLAVPSIGVITNIGESHLEMLGSREGIAQAKCELLQSLPAD
                     GVAVVNADEPLLEPYWRELACPVITFGFSRKAVIRPAAGKTGGKAKKVQIEQAGYPPL
                     VVTPPLPGRHNIYNLLAAVAVGRALELTNAEIEAGLAQLQLSGMRLEITTLPVGYTVI
                     NDAYNASPTSVAAALNVLREEAGEHGKIAVLGDMLELGALEEEGHRQVGRLAAASRLK
                     GLVVLGPRSPWIAAGAQEAGMAAEMIVQCTTHVQAAEAVELLAQAGDWLLLKGSRGMQ
                     MEEVLKLLQRVEA"
     gene            complement(26070..27575)
                     /locus_tag="VFC74_07855"
     CDS             complement(26070..27575)
                     /locus_tag="VFC74_07855"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZK25286.1"
                     /translation="MKTLAELVGSLLHAEVRGPLDRPVTGISYHSARVQSGDLFVCLP
                     GTRSHGKRYIKEALAAGAVAVVTDVHGFTAEQATVITVPNTRLALALLAACYYGYPAT
                     ELVLTGVTGTNGKTTTTHLIDALLQSDGAATGLLGTVGYHIRGQEFPSLATTPEASDL
                     QALLRQMVKARVTHVTMEVSSHALAWYRTIGCDFDTVVLTNITEDHLDFHQTFDHYLA
                     SKSKLFAWLGSLPVKKKRMRRAILNGDDPYWRQLAEQTPGEVLLYGLSAHCHVRASDI
                     QVTRDGVNYHLETPEGQLDLNLKMTGLFSVYNSLASVAVALVEGLGLGHIKAVLEEIT
                     GVPGRFEVIDEGQDFTVIVDYAHTPDGLENILRAVREFAQARVLTVFGCGGERDRSKR
                     PLMGEIAGIYSDFCIVTADNPRGEEQTQIFKEILPGLQKENKSDYLVLPDRKEAIESA
                     LSMAQTDDVVIIAGKGHETQQIFRNYTIPFDDRLVVKAWLQRRLTNNGDQL"
     gene            complement(27717..29834)
                     /locus_tag="VFC74_07860"
     CDS             complement(27717..29834)
                     /locus_tag="VFC74_07860"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZK25287.1"
                     /translation="MSRYTISNITVKKRLLFLFLCVIALVAALIIRLAWLQLVRGPEL
                     QSKAWEQWTRANPARAQRGDILDCRARLLAGSASSLSIMARPNQIKEEDKEEVARKLA
                     PILEMDEGRLLELLKQKADSVYLKRQMPEQVAQEIRKLEIRGIHFSPEPKRYYPHGSL
                     ASQLLGFVGLDEGLAGLENQYEAELKGQDGRIEMQTDGKGHQVPQGIEIFIPPVDGYT
                     LELTLDQNIQFIMEREMDRVMLESRPHGIVAIAVNPKTGAVLGITGKPDFDPNNYGEF
                     AQENWKLTPVTNTFEPGSTFKLVTLSASLEEGRYNASEGFFCSGYAKVAGASIGCWTR
                     GRGGHGAIDFTEVVLGSCNPGFIELQQRIGADTLMNYVKAFGFGQKTGVDIIGEGTGI
                     LFSPKQYGPVEAATTAFGQGVSVTPIQQVMAVSAMINGGYLMKPYIVKEMRDSEGTII
                     TKKEPQVVRRVISSETSEEVKRIMELVVTEGGGMNAYIEGYRMGGKTGTAQKVGPNGR
                     YVDGQYILSYIGFVPMEDPQILLYIAVDAPQVGPQWGSQVSAPMFKRMMESILNYLNI
                     PPDTTPEETVPSMVEVPNLIGQAMDDHTNALLENNGLLVRYIGEGPTVLNQTPKAGAK
                     VPLHTQVLVYLGGASEENEITVPELTGKTMREVGEVLGWLGLRLNSSGSGVAVEQEPA
                     ANTKVAPNSVISVKFAAPDAERR"
     gene            complement(29891..30268)
                     /locus_tag="VFC74_07865"
     CDS             complement(29891..30268)
                     /locus_tag="VFC74_07865"
                     /inference="COORDINATES: protein motif:HMM:NF016860.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HZK25288.1"
                     /translation="MLVAHKRPQEYQHSRRQRNMSRAQNRRQINLHAATYKLKLFATI
                     FACAVMAIAVISHFTQVVEAARQVELARSELQTLQEEGKHLKLSIASLNSLERLENEA
                     YELGMQYPGREQMIILTAEAAEN"
     gene            complement(30271..31230)
                     /gene="rsmH"
                     /locus_tag="VFC74_07870"
     CDS             complement(30271..31230)
                     /gene="rsmH"
                     /locus_tag="VFC74_07870"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010965431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HZK25289.1"
                     /translation="MRFSHEPVLLQEVMQVLNPQPGEVFVDCTVGGGGHSRAILEKIM
                     PAGRLVAIDQDSEALSAAKKNLAAWSDSVHFVQRNFSQLEAILTELGIDAVDGILLDI
                     GVSSHQLNEDERGFSYHANAALDMRMSREDTRTAADLINYLDASELTRIIRDFGEELW
                     AKRIADFIVAKRVANGPIETTTQLVDIIKAAIPARARRRGPHPARRTFQALRIAVNDE
                     LGVLERALEQSITYLKPGGRLAVISFHSLEDRIVKKMFQHAAAGCECPPGLPLCVCGK
                     EPQIKILTRKPLQASDRELKANPRARSAKLRAAIKVLNKGKGE"
     gene            complement(31279..31710)
                     /gene="mraZ"
                     /locus_tag="VFC74_07875"
     CDS             complement(31279..31710)
                     /gene="mraZ"
                     /locus_tag="VFC74_07875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HZK25290.1"
                     /translation="MFMGEYQHTVDGKGRLIMPAKFREELGDSFVLTRGLDNCLFVYP
                     LQEWTALEQKLKNLPFTRSDARAFTRFFFSGAAECELDKQGRVLIPTNLRNHAKLQKD
                     VVVIGVSSRVEIWSQEVWQRYSEETDLSFESIAEKIMDIEL"
     gene            complement(31967..32137)
                     /locus_tag="VFC74_07880"
     CDS             complement(31967..32137)
                     /locus_tag="VFC74_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25291.1"
                     /translation="MEALDGKVEFLCLGCGLQGLVSWSEINVEDILCPKCGDRVITSH
                     LLKKEDYALLTV"
     gene            complement(32324..32863)
                     /locus_tag="VFC74_07885"
     CDS             complement(32324..32863)
                     /locus_tag="VFC74_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HZK25292.1"
                     /translation="MIFKKRGPVNTEQALQLAVERGRELGIRHYVVASNSGKTAQQLL
                     DLAVPVVCVTHHTGFRNPGEQEMDTEVRKKLEARGVKVLTTTHLFGGIDRGVENKFGG
                     TYPGGLVAATLRMLGQGVKVGVEIAVMALDAGLIPHDEDIVAIGGSGAGADTAMVLRT
                     AHAKDFFNTRIKEIICKPA"
BASE COUNT         8886 a         8679 c         6633 g         8776 t
ORIGIN      
        1 cgttgattaa gtcgctttag taaaaactca tccggctttg acggcccttc cacaacgctt
       61 cccctcctct atagattttc ctgatttgct tcatatatta cagttttccc aaactgtcca
      121 atctcaagca aaataattgc ggctgcatag taagcatcta cgctccaatt aaaaaacggg
      181 cgaactgtaa tgttcgcccg taacatattt attacgcatt aacgctttct gtgcaacgag
      241 ggcacagtgt aggatgagag gggtctgtgc caagctgagg atggatcatc caacaacgtt
      301 cgcatttttc ccctgctgct gttttcactg ccacgcctaa accttgctca actattgttt
      361 ctgccgtccc agaaccaggc tcgtgaagag tgcagtcaga aacaatgaat aattctgcca
      421 ggcgctcttt aaagggttgc aaagcagcat aagccgcttg gtcgggatat agttccaaag
      481 ctgccgttag tgatgagcca agacgttttt ctttacgcgc ttcttccagt cgtctagtta
      541 ccacatcacg gtaggttaat aaagcttgcc aacgagtagc cagtgtttca tttttgtaag
      601 caacgtttac ttgcggccaa tctgccaatt gcaccgagcc cacatcttta accggcaaat
      661 agttccacgc ttcttcggcg gtaaatgtta atactggagc aattaactta accagggtca
      721 gtaagatttc atacattact gtttgcgccg agcggcgtgc ttgcgaattc gccacagatg
      781 tatatagcct atcctttaaa acatcgaggt agaaattaga catttccacg acacagaagt
      841 tatggatcgc atgaaagacc acatggaaat caaagtcatc ataggcacgg ctaatacgtt
      901 caactaatac ttgtaggcgg tgcagagcat aacggtctaa ttcatccaaa ttttcataag
      961 ctacagcatc gctggcaggg ttgaagtccg cgcaattacc cagcatgtag cgcatggtat
     1021 tacgaatctt ccgatagact tcagataatt gtttcagaat gtccggggag acacgcacat
     1081 cacttttgaa atcagatgat gatacccaca gacgcaaaat gtcggcacca tattgcttaa
     1141 taatctgttc gggagccaca acattgccca ctgatttgga catcttacga ccttcaccat
     1201 caactaccca gccgtgggta agggttgcct tatagggggc acgtcctcgg gtagcaacgg
     1261 aagtcaacag tgatgattga aaccaaccgc gatattgatc tgaaccttcc agatataaat
     1321 cggcaggtga aactagatca ggatgtgttt ccaaaaccgc agcatgagtg gagcctgaat
     1381 caaaccaaac atccataata tcagtttcct tacggaattc atgatgacca caggctgggc
     1441 aaacagttcc ctctggcagc agttcactgg cttcccgagc gaaccaggca tccgagccct
     1501 cttgggcaaa gatctcagca accttacgaa tagtatcctc attgatcagt tctttgttac
     1561 aagctgtaca gtaaaaaatg gggatgggaa cgccccagac acgctgacga gaaacacacc
     1621 agtcatgacg atcaacaacc atattatgaa tccgctcttc tccccaggca ggaatccact
     1681 tgacatcttt aatcgctgcc aatgtttctt tacggaaacc gtccaccgaa gcaaaccatt
     1741 gttctgttgc ccgaaatagt accggctcct tgcagcgcca gcagtgggga taggggtgct
     1801 taataaatga cagttttagc aaagcgccct ggtcagctaa agcttgagtt accgccttat
     1861 tgcctgcgtc ataatgcagg ccggcaaact gaccggcttc tgccgtaaaa tggcctttgt
     1921 gatccatggg ggcaaaaata tctaaaccat aacgcagacc aacctcatag tcatcctgac
     1981 catgtccagg agcagtatgc acacaaccag tacctgcttc cagggtcaca tgatcaccaa
     2041 gcactaccag agaagtccgc tcgtacagcg gatgctgaca cagcatacct tccagatctt
     2101 taccaagata tttttccttg attgtgtaat ccgtaatttc acaggcctgc agagcctctg
     2161 ctaacagacc ttcagccaag attaaatcac catgttcggg ggtgctgacg cgaacataag
     2221 tgtattctgg atgcaaacaa atcgccaagt tagcaggaat agtccagggt gtggtggtcc
     2281 agatgacaaa ccaagtgttt tcagcctcag caagtttgcc tttagcatct gcgatcggga
     2341 agcggacata gatggaaggt gaacgatgat cttggtactc aatttccgct tctgccagcg
     2401 ctgtttcaca ttgcgggcac cagtgtaccg gcttaagacc cttatagatg taacctttct
     2461 ccgccatggc accaaatact tcaatttcct tggcctcaaa ttctttctgt aaggtaatat
     2521 aggggttctc ccagtcacca cgtaccccta aacgtttaaa ttgttcccgc tgtaaatcaa
     2581 gaaaacgtaa agcaaactca cgacaatgct gacgtaactc cagacttgag acttcattac
     2641 gctttacttt gccagacttc agcacggcat gttcaatggg cagtccgtga gcatcccagc
     2701 ccggtacata gggcgcatca taaccaagca tcgtacgatt tttgacaata atatctttta
     2761 gcaccttatt cattgctgtt cccaaatgaa tattgccatt ggcatagggc ggaccatcat
     2821 gcagaatata tttaggccga ccttggcgat gtgcttttac ctggccgtaa atatccatct
     2881 cctgccactt ttgcagctgt tctggttccc gcttcggcag gcctgcgcgc ataggaaaat
     2941 ccgttttagg cagattaagg gttttggcat aatctttttc ttccattttt aaacctccca
     3001 gtgatattta tcaatagttt aaatttgctg tttacgcaaa aatcccgtcc tgctagagga
     3061 cgggattttt cccgcggtac caccccgtta gccgcaagct aataattgcg gccacttaaa
     3121 ataattaacg gctttcgccg gcagcggcta ctaaagttca cctgcaactc ccgggtgata
     3181 ttccacaaat ccctgcaccg gtttccacca gctccggctc tctgtctgca tgcgtatttg
     3241 tgtactcgtc ccgttcatag tttttgcgat atataattaa taccattagt ataaacaaaa
     3301 tgacctaggg atgtcaagca gactgcttct tagttatgca cagacaaaga cgcagcaact
     3361 ggggtaaggg caaacaaagg gaatgacctg cgatagatca ttccctggaa ctgcagcaac
     3421 cctgtatcca gcggccaact acaagttagg caccctgttg aacgccgtgc tctgcttgag
     3481 gcgacagcca atcttccatt tctaaagctg ccagctgcgc ttctacgagg gagcgaaaac
     3541 gcattttgta aatttgcgcc tgtttgtaga cttcatcatg ctcagaaaga atccggctag
     3601 ctttatagcg tgcctcttcc aagagccgcg ctgcatcttt ttcagcttcg cggcggatta
     3661 aatcagcttc cttagatgcg tttcttttca cttcttccgc tgtttcctga gccaccacaa
     3721 tcgcattatg taaagtatgt tccagttttt gataatgagc aagcttttcc ttatgctgcg
     3781 atatttgctc tttagccgta atattatctt taatcaattc ttcataatct ttcgcgaccc
     3841 gattaagaaa ctcatcgact tcgttcgcag ccagtccacg gattgaacga ctaaactctt
     3901 tgttttggat atcaattggc gttaaaggca cttttatcca cccccgctta ttttccctca
     3961 gttccgctac cagaaaagtg cgcttaagca acaatctgac gcctcaatgc tggttatgtc
     4021 tagacgtttc actaaaacca atatttaacc aatatttctc tttagaggat aacaaatcct
     4081 gctaactaga gatgtttttt taatagcagc tgaatacggc cctttttact ttgacccaaa
     4141 acttgtgcta cttcaactcg cccccggccg ctgaccgcaa ttacatctcc ctctttaatg
     4201 gccgcactgg cactcttcac cgattgccaa ttaagtttca catgaccggc gcgaattgcc
     4261 ggtgtcaatt tgctgcgtga taaaccaaag ccgcagctgg ccacggcatc taaacgcaaa
     4321 ctggcaactg tcgttttaat ttcacgcaca cgacctactg tcggtgaaag ttcagccgca
     4381 gtaatttcac aaagctcgag attatacttt gctatctgct gccagttggc aagaagaaag
     4441 ttcaacaact cagggtgcac cactacctgt gctgaagttt cgctggtcac gataatgtct
     4501 ccaaccttct cacgccgcaa gcctaagccc agcaaagctc ccagccagtc acggtgtgag
     4561 aggccagacg cttgagcggg tgcgtttgcg gccagaaagg caaggggtac atcattaacg
     4621 gtggcctgtt taacagcaga gaagaccatc agacgtgctc tttctgctgc ctcatagcca
     4681 ccccatacca cctgtttgat atctttaaaa tgatggagaa cccgatcggc gacacgttgt
     4741 tgaaagggat ccagaaagtc tgtcgtctgg ggcttgcccg tttgctgcac ttgtgctagc
     4801 ttatcgagca cagtacacgc cgttattttt tcaatttcct gctcaaattg cttgagaatt
     4861 ttttctcgct ccattgttta cctccatact ttagtaacaa ctggagcatg ctaaccctgc
     4921 tccttattct ttatccagat ctgtcactac acctctattg cttaactcac gacgcatttc
     4981 agcatttact tccacttgcg ctggggcgaa gacaaatatt tgctgactta ctttttgcat
     5041 gttgccttcg agggcgtaag ttgcaccaca aataaagtca acaattcgtt ttgcttcacc
     5101 tttatctgtc tcttccatat taaccaagac aggcttgcgg ttcttaatat ggtcaacaat
     5161 tgcctgggct tcagcaaatt catatggttt agtgattaca agccgcatat tctttgcggt
     5221 atgtagattt aaaaccgttc ctttgcgcgc agatgactgt ctgggattat cttgttcctc
     5281 gtaagtctct tcttcctcag cctcttccat caaaccaaaa aatgttaatg ttttctccca
     5341 aatcgaagcc actttcaatt ccccctattt aggctctttc gccaaaaagt aagctgccaa
     5401 tgcgtactaa atttgcccct tctgccacgg caatctcaaa gtcgttggac atacccatgg
     5461 agagagtctt catgtgcact cctgggattt ggagcttggt aaacagctga tacatacttc
     5521 taaaataagg acgcgctgcc tctggatctt cactatacgg cgggacagcc atcagcccct
     5581 caacttgcaa gtgcgagaag gtagaaagct catggataaa ttcagccact tcataaggct
     5641 caagcccgcc cttctgtgcc tcgccgccaa tatttacctg tactaacgtt cgcactcgct
     5701 gttccctttt tgctgcttct cgctgtaaag cctctgccaa actccagcga tctaaagaat
     5761 gaattagggt aaagttacca accacatctt ttactttatt ggtttgtaaa cgcccaatat
     5821 agtgccactc ggcctgtggc agatgtgcca gcttaggtag agcctcctgc actcgacttt
     5881 cgccaaacgt ttttaaccct aaagaaaatg cttgtgctat acactcgctg ggcacttttt
     5941 tggtcaccgc taaaagctgc acaggcttgt tcttagaccg tcgtgcggcc tgctgtaaac
     6001 gctgctgtac cttggcaatt tgcttagcta aatcactcat aatttctggc cctctttgac
     6061 gaacacagga ttaatgataa ccggtatgcc tgtgggcaga ccatgcacca ccacctgctg
     6121 ggcttcagtt gttactactt ctacttttcg atagcgtacc agggactgat ccaaaacata
     6181 aacaccagtc tcatcaccac gtacacgtaa agctgatgtc ggcagcacaa tgcctctggt
     6241 gcgctgagta acaatttcta cctcggcaaa gcgctcctca taacagccag ctaactgctc
     6301 ctgcagagca aacacagcag tcacagtgcc ctcttcttct ataacttccc tgatttgggc
     6361 acgaacagtt gctgaatcgt ccagcgaaag ccgtaaatct accgtcgttt tcccttctag
     6421 cgcggcaaaa gcatcatgct tgaattgtgc tacatagtac catagataat tattgatgct
     6481 tttaaaaata aaagaaccct gctgcacttc agcaccgtcg tctacttcct gaaggtcagc
     6541 cttcgcttca cttaattgcg cgacatccag agctgcgggc tgctccagga taactggctg
     6601 cagtgcaccc tcaaagccat ctatcttttt tataaccatc cctgctgctg gcatcgtcac
     6661 tgtttgtaca gtacccccgg ccgtggtaat cgtggctacg ggggcgccaa tggccaaacg
     6721 ctcaccatcg gcagccagcc actttaatgt tcctgttaaa ggtgccgtca cgacttgctc
     6781 ctgacggaca atgaaacctg gagcctccac aatatgctcc agtactccta tctgcgcatt
     6841 aacagtacgc acaaagatag ctgccagggc ctgatgtgca aatggccaag caatgcgtac
     6901 taccagcaga gcaagcgtca gcagaattaa gctttggatt ttaggtcgct tgctttttgt
     6961 tttctgcccc tcaattactg taaatcttct acgttttcgc atattattcc taccacttca
     7021 ttgaaatcac accggccatc cggccagttt gtccaccggc agcacggtac gaataaaata
     7081 agtctgtatg acaagctgta caatagctgc tcttgacaag attttcttcc ttgatacctg
     7141 cggctcgcag ttgcagacgc tgaaattccg gcaaatcaaa gtagtaggcg ccagccgccg
     7201 gttgtaagac ttcagcgcgc cagatttgcg gcacctgctc tgccacattc tcacccacct
     7261 ggtaacagca tggcccgata gtaggtgcta aagccacgcg gagattttcc gggcggctgc
     7321 ctaatttgct tagtgcttct accatcagcg gccccatacc agccatagcc ccacgccaac
     7381 ctgcgtgggc caaaccaata caacgtacca ctgggtcata gaagaaaagc agcgtgcagt
     7441 ctgcagcatg agccatcagc acaatttctg gttcagtaca aatcaaaccg tcagttgccg
     7501 ccacagcatc agtcatctgg taatggccgc agccggccat ctctggcgta acacgggcaa
     7561 ttgctgtccc atggacttgt tccccgacga cgacagtact agtttggtag gtaagtgctt
     7621 ccgccgccag ttgacggttt gtgcgcacgt cctgctcgct gtctcccacg tgcagtccca
     7681 tattcagact gtcgtaaggc ggcgcactta caccgcctga gcgcagagta accccgtgca
     7741 gcaagccagc gcactgcaga ttttcaaact gtagataacg tatagccgtc actggagata
     7801 ttttttcttt aggcatgctt ctgcacccca cgatagtgta actctgacaa aaccaacata
     7861 cccgcttgga tgctggcccg cacatcaata attctttggt tgcctgagcc gcggtagagt
     7921 aagaataaat ctctttgctt ctccaggtaa ggcccatcaa tcaataaatc gctggcggcc
     7981 aagagcatcg cagtaccgac atcttttttg gcttgttgac tcagctgttc atacgtataa
     8041 ccactataaa ctgtcaggtg cagtaaaggt gaacgtgctt tcaacagtgc cgccagacta
     8101 gctaaagccc gcgcctgtag aaaaggctca ccaccagaga aggttacacc atgcaaatta
     8161 gtttcagcta ggatttgtgc tgcgacttca ctaatttcca cacattgccc ctctgcaggg
     8221 ttatgcgtat gtgggttatg acacccagag cagttgtggg ggcagccctg agtaaacact
     8281 actgtacgta taccaggacc atccaccaca ctgtattgat gcaaacctgc tagattaagc
     8341 ttcataatac tcctccaaag tcactgccta ctagtgtaaa tgtgctttac ggtcatgtag
     8401 ttcagcctgt ttagcactat taaaacgctc ttcagtagat aaatatccgg tgatccggcg
     8461 aatccggcgt acattagtgc cgccgcagct ggggcagcaa tcatgcataa tgccgctgaa
     8521 gccacaagta cggcattcgt caatgggata gttaattcca ccataaccaa catcgctggc
     8581 cgccatatgg cgaactattt cttccaccgc ttcatagttc tctccgggtg gagcttccaa
     8641 ctccacgtag gcaatatgac cggcatcaca ataattatgg tatgccccct caatgctcaa
     8701 cttatcaaat aaagaaatgc agtagctaac tggcacatgt acagaattag tatagtattc
     8761 cttgtctgtg accttgggaa tgataccaaa ctgctcacgg tcgattttta tgaaccgacc
     8821 cgataaacct tccgccgggg tagctactag cgtaaagttt aaatcaaatt cctccgaaaa
     8881 ctgcttcacc cgttttgaca tatgagcaac aatttttaag cctaaatctt gtgcttcctg
     8941 gtcctcacca tggtgcttac cgatcagcac ctgcagtgtt tcggccaagc cgataaaccc
     9001 tacagaaagt gtaccatgct taatcacttc tttaatcgga tcgttggggc ctagcgcttc
     9061 agaacccata tacagctttt gccccattaa aaacggcaag tcttttgccc gcaaattgcc
     9121 gagaatttca aaacgatgca gcaattgacg agccgctaaa cgcagcagac gatctaactc
     9181 cacaaaaaaa gtattagtat ctcgtgcctg cagggctaaa cgcggcagat ttaaggaaac
     9241 cggcgcgata ttaccacgac ccgcggcaac ttcgtcacca tgacgattgg caatcacccg
     9301 cgtgcggcag cccatataag ctacttcctc cccgtatttg gcattaaacg tactatccat
     9361 aaaactaaaa gtaggattta aacgtttcgc cgccacttta agggcataga caaacagatc
     9421 ataatttgga tcttctggat taaagtttac accttttttg gtccggaaga caagattggg
     9481 gaaaataggg ctctctcctt tacccaaccc tttctcaaac acttgcaata tagccttggt
     9541 aacggctcgt cccattggtg tggtagctgt cccaatgtta agtgaagaaa agggcacttg
     9601 ggctccggcc cgggagtgca tagtatttaa attatagact agtccttcca tcgcctggaa
     9661 aatttcattt tcatctggtg tggtaggcat ggtggcaata atttgcgaca tggaccggtc
     9721 gaaatgagga aaactttgcc caccaaacat atcattttgg ttactttgca ggataatcgc
     9781 tgcttgtgct accgctgagg caatgcgttt aggtggacga atataaccat accctgtatc
     9841 aaagccagat gagagcagct taaacaaatc tatctgcagg caatttaagg ttttaccata
     9901 ataatctaaa tcatgaatat gaaaaacgcc atttatatgc gcttgcgagt actcttcagg
     9961 cagcaaatta gttaaatagt aatgtttgct agccgcactg gcaatttgca gcattttggc
    10021 tgatggcgaa ttagaaacgt tggcattctc acggctggtt tcaattaaaa tttctttaac
    10081 tgtatccatc aaatcacttt taccttcacg gatacggttc cgacgatcgc ggtacagtat
    10141 gtacgctttt gctgtacggg catgtccatt ttcaatgagc actctttcca caagatcctg
    10201 gacatcttcc actgccggaa taataccgtt ataacctaat tgttccaagg ccttaatgac
    10261 ctggcccgtt aagttttcag ctatcttgcg attatctccc cccaccgctt tagccgcctt
    10321 gacaatggca tctgtaattt tttcagcatc aaacaggaca acacgcccgt ctcttttacg
    10381 aatctctgta aacattgcta ttcctccggt cgctagttaa cttgttacgt tcttaggcgg
    10441 catttctgcc aaggcctttt taaaatcatc aatattggta aacttgtgat aaaccgaagc
    10501 aaaacgtaca taagctactt gatcaatttt atacaactta gccaaaagac gcttgccgat
    10561 ttcatcagaa gttacttcct gtgaaaaact attacgtagt tcacgttcca actcatctac
    10621 taactgctca aaaacatgaa gagggatctt ctgcttgccc cccgcataga taaggccgtt
    10681 taaaactttg gtccggtcaa acaattgccg gttaccattg cgtttcacaa caattagcgg
    10741 cgtttcctct agtttttcgt aggttgtaaa ccgcctttcg caaactgtac actcccgacg
    10801 tctgcgaatt gcactgcctt catctgtcga acgagaatca ataacacggc tttctacaca
    10861 accgcagtgc gggcatttca ttggttcctc accccctgtt gcgctttaac actatctggt
    10921 taataaagta aacaattaac atcttgtacc aaaacaaata ctacatctag tgctggtata
    10981 attatacaac accagatata gtatatcaag cactattctt cttttgtaaa aattcttaat
    11041 aacgactgct gctagttggc cctatcaatt ctacaagaat agtatctacg ccgaacttaa
    11101 ctatattttc ccaaggaata atgtaatctc ggccgccacc caaaaagcca aacaaacgag
    11161 tagctccagg aacaataatg gcagcgatcc ggccgtgctc taaatctaaa tcaaggtcac
    11221 aaatcacccc gagccttttc ccgtctacaa tattaaccac atcacgattg cgaaggtcgg
    11281 agatcttaac catagtacac ctctactttc atgccttttc ctacagtata tgcaagactg
    11341 tttgtccagt ggacataaaa aataaattgg ggatttgcta gtcctgatct gcacatgaaa
    11401 aaagagagct ttcgctctct ttttagtaat atgcaagatt aatctggtga ttttttatgg
    11461 ccaaaactcg gagacaggag caacacgccg atagtagtag tgaccgtcta cttcacggac
    11521 ttcagtcacc cgaataaggt tagactggtt aaaagcctgt acggcttcgc cgggaggaga
    11581 caggaacacc ataaagagtg taagcactag gagcatgcta acccctactt gccaggcata
    11641 tctcttgtgc acctttctcc acctcctttt ttactttact gcacgtgttt acgtagatgg
    11701 cctaaagcag ctttttccag gcgtgaaact tgagcctgcg aaatgcctat ttcatcagct
    11761 acttccattt gcgttttgcc gcgataaaac cgtaaagtga ggattaattt ttctcgttca
    11821 ttcagtttct gtaatgcttc tttgatagct atgacctcaa gccacttttc atcctgattt
    11881 ttatcatcag agatttgatc catgacaaaa atggggtcac caccatcatg ataaatcggc
    11941 tcaaataagg acactggctc ttgaatagca tctaaagcaa agacaatatc ttcacgcttt
    12001 aaatttaact ctttcgcaat ttcactgatg ttaggctccc ggccatactg gttggccaag
    12061 gagtcacgaa tctgcagtac tttgtaagca atatcacgca gagaacgtga gacacgaata
    12121 ggattattat cgcgtaagta acgtcgtatt tctccaataa tcatgggcac agcatatgta
    12181 gaaaacttga cgttttgtcc aagatcaaaa ttatcaatgg ctttgattaa accgatgcag
    12241 ccaacctgga acaaatcatc tacatattcc ccacgattgt taaaacgctg gatcacactt
    12301 aacaccaagc gcaagttgcc atttactaac ttttgccgag cactctcatc accggcttga
    12361 tagcgagtga ataagcggcg catttcatca tttttcagga cgggcagttt tgatgtgttg
    12421 acaccgcaaa tttctacctt gttgatcaag ctcaatcccc tcccggatta aaatgacaag
    12481 gaataaatca cctgtccttt ctatctgcat tatttcctga gaggatattt ttattcagct
    12541 aaagattccc agtcctgccc agatgcgagc tattccattt tttttatctc ttttttgaga
    12601 cgttttataa tccttttttc taggcgggag atgtaagact gcgaaatacc caaaagtacg
    12661 gccacttctt tttgtgtttt ctcttccttg cctagaagcc caaagcgcag ctccataatc
    12721 cttttttcgc ggctgcttaa cttttcaatt gctgtataca aaagcttccg ttccacttct
    12781 gcttcaatat tcttgatcac taaatcattt tctgtgccta aaacatcaga aagtaataac
    12841 tcgttgccgt cccaatcgac atttagcggt tcatcaaacg aaacttccgc tttaacttta
    12901 ttattcctcc ggagaaacat taaaatttca ttctcgatac atttagaagc ataagtagct
    12961 aattttatgt ttttgtgtgg gtcaaaggta ttaacagctt taattaaacc aatggtcccg
    13021 atagacacta aatcatcaat atgaatacct gtattctcaa atttacgggc aatatatact
    13081 actaggcgca ggttacgttc aatcaaaact gtcttaactg cataatcacc tagttttagt
    13141 ttttctaata aaaagacctc ttcatcctgc gttaagggcg gcggtaatgt ttccgaacta
    13201 cctacataat atatttctgg taaagtttcc acccctaaac gatgtaaaaa acgcagatat
    13261 gccaagcgca atcttaattt ccagtgctgc agtaacctca acaccccaca cctcccatca
    13321 cttcgaggat aaacgggtgt aataaaactt cagtatcgtt atctagtgac agcggtcttg
    13381 ctgtcaaagc aaaaaccaaa tcgcttctct cctctctctg attttgatgt tcaacaatca
    13441 ctagcggtgg gcgataagtt accaacatac cactgttctc taaagaacga tacaacacta
    13501 ctccaaaacg tatcggctct tcacagttta acagccccag cgcagaaacc ggatcctgac
    13561 ccgtttcata tgctgtacgg aggacttgtg gcagcagcgg acgcaaagcc tggtaactgg
    13621 cgacacaaag aggcttgccg gagagaggat cacgcagctg attgccggta tccactaacg
    13681 cccgtacagt tacctcctgc tcggcatcgt gaatcagtaa acgaaaagaa aagtcctttc
    13741 tttttcgctg ccgttcacta aaatgccaga ccatcatcag caagctaaag gctactagca
    13801 cgccgctaaa aagcatcccc ggccgcggcg gctcaatata aaaaataccg cctttaacca
    13861 tggctggtgc actgccaaag aaataaaaag caaaaattgt ccctgccaca aaaaatgaaa
    13921 tcaagaaaaa ggcaccgcat aagcgaagta attctactgg ccgctgaggc ttaaaagtaa
    13981 aaaggattac gacgatagag gcgacaattt ttccggccca agagaaaata atcacagagg
    14041 ccggcaaaaa aatagccagg ctataaagag ccgcgaccaa tccaccggcc aaaactcgtg
    14101 ttcgtgttac ctcccgccct gttaaacgtg ctgttaaata cagcaaagaa gcattcatca
    14161 taaaatttag ggcaaggagg tcctcaatgt acatacgctc acctgccctc acattctctg
    14221 gacaaactgc tacattatat gctcgccccc agccatctat accattataa accatcacgc
    14281 catagtaatt tgtcagacgc tggtgtcgaa taatgttttt gttctggttc tgtggttctg
    14341 gggtcaggct tgaataattt acttcgccag aaggaaaaga agtcggaaaa caaaaattgc
    14401 caccttgtct ggttaacaag gtagcaatga gtaattcaac aattcaagcc tgaccctagc
    14461 gtttgcggcg caagaaagca gggatgtcaa tatcatcgtt gttaaaggtt ctttgtgtca
    14521 gctcatcaat aatttgttta cgctcaccgg cacgatgatc aaagccggta gcaataacgg
    14581 tcacccgcac ttcatcttgc aggctttcgt caatgactgc accaaagata atgttagcat
    14641 caggatctgc tgcttccgcc acaatatcag cggcctcgtt aacttcaaag agtcccaaat
    14701 cagcgctgcc ggtgatgttt aaaagaacac cacgcgcccc atcaatggaa gtttccaata
    14761 acgggctggc aatcgctgct ttagctgctt ctactgtacg gttatcccct gaagcaacgc
    14821 cgatgcccat taaggctgct cctgtttctt tcatgatggt cttgacgtca gcaaagtcta
    14881 gattaattag tcccggtaca gcaattaaat ctgagatacc ctgcacccct tgccgtagta
    14941 catcgtcagc aatgcggaaa gcctccagca tcggtgtccg tttctctact acctgcagca
    15001 aacggtcatt aggaataata atcaaagtat caactttctc ttttagttca gcgataccct
    15061 tttctgcggc agcttgacga cgcttacctt caaaagtaaa aggtttagtg acaacaccca
    15121 cggtcaaggc ccctatttca cgagagactt ccgcaattat tggtgccgca ccggtaccgg
    15181 taccaccacc catgcccgca gtcacaaaaa ccatatcagc gcccttgagc gcttctttta
    15241 gttcctctct actttcttct gcggctttct taccaacttc aggattagca cctgctccta
    15301 aacctttagt taatttctca ccaatttgta atttatagcc tgattcagcc aaatagagag
    15361 cctgggcatc tgtatttaca gaaacaaact ctacaccacg caagccggcg tcgatcatgc
    15421 gcttgaccgc attactcccg ccgccgccta caccaataac ttttatttgg gcaaattgtt
    15481 ccatctcaat atcaaactcg atcaaaagaa agactcccct tctctcagcg cagattaaga
    15541 ctcaaacata tccatgaacc agcttttgac tttgttaaaa atgcccggta aggcctttcg
    15601 cccaccacga tactcattta cttcaaacat ttgggtgtgc tgtacgtagt ggataatgcc
    15661 gaccgcagtg gaatagatag ggcttttcac ccctacataa ccaggttgag ccacgcgtgc
    15721 cgaagtttgg aaaatctgtt ctgccaattt ctctatgcca ggcattaagg aaactcctcc
    15781 cgtgaggacg atgcctgcag gaagcgcttc ctggtatcct aattgcagca attcttcgtg
    15841 tgccatttgt agaatttcta tcaagcgtgc ctcaataatc aaagccaatt ctttcgctgg
    15901 cactttacgt gcttctttac cagagatttg tggcaattca atggtcgcat ggtcggcaat
    15961 tactttggct gaagccgcac catgatccag tttaattctt tcagctgtct ctagggttgt
    16021 gcgtaatcca acagcgatat cgttggtaat atggtcaccg cctacaggca ggaccgcaat
    16081 atttttaaga taaccatttt ggaacactgc aacttcggta gtgccgccgc caatgtcaac
    16141 catcactacg cccaattctt tttcatcacg cgttagtgct acttccgcat tggctaaagc
    16201 gtttagtgct gtaccgtcga tgccgactcc tgctcgttct acacaacgat ataagttacg
    16261 tagagaggta atcatccccg taataatcat tgcatcaact tctaaacgaa caccaaccat
    16321 ccccacagga tcacgaatac cgtcgtaacc atcaacaata aactcacggg ggataacatc
    16381 aataatttca cgatcaggtg gcagggcaat tactttggct gcttgtaaaa ctctttcgac
    16441 atcttcttcc gtaatttcct tatctgcacc aaccacggcg accacaccac ggttattaat
    16501 taggcctacc tgtgatccaa caatacccaa aaaagcagag ggtattgctg tacccaccat
    16561 ccgttcagcc tcatccatag cgtgcatgat tgcttctacc gttttatcca aatcgacaat
    16621 aacacctttt tttagaccct cggaggggct tgtcccaaca ccaatgatac tgagtgaatt
    16681 gtcgacgttt acttctccaa ctatcgcgcg aacatgagac gtaccaatat ccaaaccgca
    16741 gattaagtct cttttcacac acaacacccc ctcgccgctc ttcttttaaa cataattcac
    16801 attattgttt tattcaacaa gagttaggtt ttcccttttt tttgaactaa ttgtttttct
    16861 tgtttttttc atcttttagg ggatgaaata ctggtgcggt tgctgcccgt aaatcgagat
    16921 agccttctgc ctgctttccg ttaagttgtc cggtaatttg cagcaaaatg tttgcttttt
    16981 caacaaaatt atctaccccc agccatacgc ttaaaccatc catcgtgaca ataatcaaat
    17041 tatctgcttg ctctaggtta atttctgaga tcatcactcc ttcctctttc agtgcttcgc
    17101 taagctgctg aacttgatgt aaatcctctg ctgtcagcag catctggcca actgtgccgg
    17161 ctatgggagc cacaccggta agtaaaggca ggtcatagtt ttgtgtcgct tgtgttgtcc
    17221 ccagtatttg cccattccag ccgatttcga gaaagtactc ttgaaagggg attagcacta
    17281 gcatttttct ttcttgaagc ttaatgttta gcgtccgcgg cagccgccgt gttacagata
    17341 ctgtttctac ccggggaata gcctgaatcc tttcttccaa tagcggcaga cttaacttcc
    17401 agatgttttc cccttccgta atttgcatgg catgccgtat ttcagcttca gttgtataat
    17461 tattgccgtc aaatgaaaat aactcgacgc taaaaaagtc ggacttaaca aaactgatgc
    17521 ttgtccatac ggtaagaaaa attatcagga ctatcagcag atagcgtttc agtttgtgaa
    17581 attgtactgc atctgcagta gacgttattt gacgtttgcg gcggtatttt cgttgtagct
    17641 tgtccatttg taacctccac ttgatttaac cgggagttgg cagcggactg ccaggcagcc
    17701 cgcttagcca tcagtaatac atttaatctc agcaccgagt ttgcttaaat cgcctgctaa
    17761 attttcatag cctcgcacaa tatgttctaa accatcgaca acagtttctc cttcagcagc
    17821 taaaccggca atcaccagtg ccgcgcccgc ccggagatct ggagctgtca ctgtcgctcc
    17881 catcaggtgt tcagtaccat gcacaatagc tgtgcggtta tctgaggcaa cgataatttg
    17941 cgctcccatc cgatttagtt caccaacatg tttgaagcga gaatcaaaga cattttccac
    18001 aatgatgctc gtgccttgtg cccgggtgag caccgccatc attggtgcct gcaaatcagt
    18061 aggataacca gggtaaggca aagtacgtac atactctaac gcgcgcagcc tttgtggtgc
    18121 ctgaacaaat aatcgtccgc tatcttcgcg aatctggaca ccagcttcgc gcaatttagc
    18181 cgttaccgca tctaaatgtc tgggtatcac attttccaaa atgatgttcc caccagtaat
    18241 ggcggctgcc atcatcatgg tgccagcgac aatacgatca ggaatcacgg catattccac
    18301 accatgtagt tgtgtgacac cttctatgcg gatgtggtca gtgcctgcac cattgattct
    18361 agcccccatt ttgttcagaa tattttgcag atcaacaatc tctggttctt tcgcagcatt
    18421 actgattacc gttacaccac gcgcgtagac ggcagctaac ataatatttt ctgtagcacc
    18481 gacactggga aagtctagat gaatatcagc accaactaaa cgtgaacatg aggcataaat
    18541 atagccatgt tgttctcgta gttgtgcccc caaagcagcc aaacctttta aatgtaaatc
    18601 aatggggcgc tggccgatgg cacacccacc ggggtaagag atgcaaactt gaccaaagcg
    18661 ggctaaaata gctcccatta aaaagattgt ggagcgcatt tcgcgcatta aggcctcatc
    18721 aattgcaaac cccgtaaggt tgtgggtttt tatcgccata tctgtaccgc tccaggtgat
    18781 ttctacacca agactgcgta atatttccac catttttatc acgtcacgaa ttcgcggcac
    18841 attagttaga aacacttcat caccgctttt gttcaataaa actgctgcta aaatgggtaa
    18901 aatagaattt ttagcaccgc caatgttgac gaccccttct aaacgcttgc caccacgcac
    18961 aataaacttt tccatgcttc caccttcccc gttaatagcc gagcaggcga acctccaatg
    19021 ctaattcaat accaaactga cgtgatactt cttgctgcac cttgctaatc aacgtcatca
    19081 catcttgagc cgttgcgtgt tgttgattaa caataaaatt agcatgaaca tcagagatgg
    19141 ctgctccacc cacccgatga ccttttagcc cagccgcttc aatcaatcga ccggcagcat
    19201 caccaagggg gtttttaaaa atacttccgg cattgggttg acggggttgg cggttccgcc
    19261 ggaaggctaa tgcttcccga ctctgccgca ctaattcatc cttatcccct gctgttagct
    19321 gtaacctgac agcacaaacc acctgcgggg gacgaatatt gctttggcgg tagcttaacc
    19381 ccaattcctg cggttgacag gtatgtaaaa ttaaagccgc attgagaatt tgcgcgtcga
    19441 ccaaaagttc ccccagtgag tgtccgtgcg ccccggcatt catgactaaa gcaccaccaa
    19501 ctgaacccgg tattccacca gcaaatgcta ggccgcgcag gccaagctgt tcagcctttt
    19561 gcgctaacac cgccagaggt accgctgcac cggctgaaac tagctcctgc gtaaagctaa
    19621 attcacgaaa ctgtccagcc agacgcacca cagcaccacg atagccatcg tcatggacca
    19681 gtaaattagt tccgttgccc aagaagtaat aaggcttttt ttgttcttgc agacaagtca
    19741 gcacgaaaat taattgctct acattctgtg gttcgacata taaatctgct ggaccgccga
    19801 ttttaaatga ggtatgccta ctcaatggtt cagataattt taccatttct acctttttag
    19861 ctatcttctc agcaacaaat tccatgtaac cctccacgca caacccttgc cgtaatttat
    19921 agtatgcaaa tgactctaga gtgtgttttt taccaaagtt gcgctgctgt taagttgtta
    19981 aaatgtaaat ctgcagaaat atctctcgtg tgagttgaga tatgttattt tgttcagaac
    20041 agccgcagag ttttagcggc aaacttcatg catcacagca gtcatccgcg ctgcggcatc
    20101 gggtatgcct aaacctttac tagcctcagc catcgcttgc aggcgttctt tatcactcaa
    20161 caagccgacc agctggtccg cgagcacagc agtagttaat tcctgctcac gaataagtac
    20221 agcggcatca tgctcggaga gcatgcgggc attatgttct tgatggttat tagtaacatt
    20281 gggagaagga attacaatgc aggggacacc taatgctgtc aattctgcta aagttgtcgc
    20341 accagctctg ctgaccatca gattagtggc cgccaaaaga cgcggcaatt gttcgtgata
    20401 cggcaagata tgcaggcgct gaggaaattg ctgctgcaaa ggtgccaatt gagcagtcag
    20461 ttcttcataa tagcgacgac ctgtcacata aaccacctgc gccttggtct cacttaacac
    20521 acgcactaaa ctgccagcaa aagcttcgtt cagttttagg gctccatggc tgccaccgac
    20581 acataacagg acaggtaagt taggctgcaa gacaggttct tgcagcggag caccactggc
    20641 agcactagcc gcctctgttg ctctgggatt gcctgttaaa atcgttttct ggggacgagg
    20701 aaaaaatttc tcactaccag gcatgcttaa acacacccgg ttgacgatac gtgagagcca
    20761 gacattagtc aacccaggaa tagcattttg ttcatgaata acagtcggta tccctgccag
    20821 acgcgccgcc agcaccaccg gtcctgccac gtagcctccg gtacccacaa ccacgtcagg
    20881 ccgaaaagag cgtatgatcc gccgggcggt gaccactgcc gcagcagcca aatagacagc
    20941 tcgtcccagg cgcagcgata aacgccgctc caggccaatc acagggattg tttttaactc
    21001 aaagcctgct gccggtacaa tttgcttttc catcccttgt tctgtaccta caaacagtac
    21061 ttctgctcct tcattttcac ggacataacg agccagtgcc agggccgggt aaatatgccc
    21121 accagttcca ccaccggtaa aaatcacttt catcgcgtcc acctacttat tttgcatatt
    21181 tagaaatatt taagagaaca cccagactgc acatggtaaa gagcagcgaa gagccaccgg
    21241 aactaattaa cggtaaatta ataccggtta ccggaatgga gccagtaact acgccgatat
    21301 tcatcagcgt ttgcaggccg atcatgctaa taatacctgt cgccagcaga ctcccgtagg
    21361 catctggtgc tgataaggca attttaatcc cacgccagaa taagagaaag tagataatca
    21421 gcacagtgct ggcgccgata aagcctaatt cttcactaat gacggcaaag ataaaatcac
    21481 tatgcggttc aggcagataa tgcatctttt gccgcccctg ccccagaccg accccaaaga
    21541 gtccacccgg gccaatggca taaagtgatt ggatgatctg ataaccagta tccagggggt
    21601 ccgcccacgg atcacgaaaa gacattaggc gctgcaggcg gtagggctca ctataaatca
    21661 aataaaagag catcggcacc gcactggcgc caattaaagc cagctgctgc agcggcatgc
    21721 ccgccacaaa gataaccagc atcaccgaac cagccatggc tacccctgtg cccaagtccg
    21781 gctgtttcat aatcatcgca aaaaagatcc ccatgataat taaaatcggt aaaggaccct
    21841 ctgtaaattt ttgcacctca atatcacgac gtgataaata agctgcggca aacataatca
    21901 aagcaacttt agctagatcc gagggttgaa aagttaacac cccaacgcga atccaacgtc
    21961 tggcacctaa aatttcctcc cctatcccag ggataaaaac cgcaaccagc ataataaaac
    22021 ttaacaataa cactaaatta gtccaacgct ttaatttcca ataatcataa ttgctgaaaa
    22081 aaagcattcc tgctaagcct aaagccgccc attgcacctg acgccgcaga tgataatatg
    22141 ggtcgctgcg ttcacgtaaa atataataac tggcactaaa aaccatcaca atgccaatcg
    22201 ccagaagtgt catggttgta aacagtaaaa tgaaatctgc ctcttttttg cggatcttgc
    22261 ggtccgacct accggccatt ttcctgcccc cttagctgcc aaactgcctg cttaaacaat
    22321 tttccgcgct cctcataatc ccgaaacata ccccaagaag cacaagctgg tgacagtaaa
    22381 accacttcac ctctctgtgc tttggcttgt gccatttgta cagcttctgc caaactagcg
    22441 gcaaaagtga aattaaagta tcccgcctcc cggaaggcgg ctgctaatcg ttctgccacc
    22501 tgtcccaaga ggatgacgtg agatacttcg ccggctaaag cttgagcaaa aggagcaaaa
    22561 tcagcacctt tttcgtagcc gccggcaatt aaaactttac gctgcggaaa ggctgacagc
    22621 gctttgagcg tagcatcgac attggtgccc ttggaatcat taatataatc gacacccgcc
    22681 agagtacgca catattctaa acgatgttct acccccggga aagtagttaa aacctgggca
    22741 atgattgctg caggtacgcc gcctagccag gccagcaagg tggcagctaa agcattctct
    22801 agattatgac tgccgggaat tgccagatct tcctgcggac aaacggttac ttcctcctgg
    22861 ccgtcacgta aagtaatcag gccatcacgt acaaaagcgc cacgctctac ttcccgtaag
    22921 cgactaaaga gataaacctg acagtgggga cgctgccgca gcgccaatgc ttctggatca
    22981 tctgcattta gcactaccgc atcagcagcg cgctgattag caaaaatcat cgctttggct
    23041 tcccgataag cttcccaaga gccgtgatga tccagatgat ccggtgtaat atttaaaatt
    23101 gctgctaaat gtggccgaaa gtgagatgta ccctctaact gaaaactact taattcggca
    23161 acgacaacat cttcagcttg cacttggggg atgacagcgg aaagcggcaa accaatattg
    23221 cccgccacaa atgtctgtct gccggtggct cggtacattt ctcccgttag cgccgtggtc
    23281 gtcgttttac cattggtccc agtaatgcca accagtggcg cggtaagatg ccaggcagca
    23341 tgttctacct cggtaattac cggcacagcc agctctgctg ctctttgcag cggcggcagc
    23401 tgcagaggga cgccggggtt cttgataatt aaggccgtct gttctgtgac aagatttgcc
    23461 ggatgcccac cgccaacaaa acgaagcgtg gaattttcct tcagaaataa ctgttcttct
    23521 gctgttaaat cttctgctgt gcgcttgtca ttaacggtaa cttgtgctcc atcctgcagc
    23581 agcaacaagg cacttgccat cccgctgcgt gccaggccaa tcactaatac ttcttttccc
    23641 agcaaatcca cgctcaatct ctccctttca gcccaactcc gcctcattgt gttcaggagc
    23701 ggttataaat tctgatgttt agctcggtga atttcaaatt agagggcacc tagcagccag
    23761 aggcctaata cagccaataa aaaggccaag gcccaaaaac cggtgactac attccattca
    23821 ctgtaaccgc ccagctcata atgatgatgt aaaggactca tgcgaaaaac tcgtttaccc
    23881 gtactctgaa aaacaagcac ctggataatt acagaaaaag tttctatcac aaagacgccg
    23941 ccaataatta gtagaaatag ttccgtttta gtcaaaattg ccagacccgc caacacgcca
    24001 cccagcgcta aagagccaac atcacccata ataacacggg ctgggtgcaa attgtacgct
    24061 aaaaaggcaa acacagcacc gatcatggct gcggcaaacc cagctaatcc cagactttgc
    24121 tgcagcacag caatcagcat atatgctaat aaagacagaa tcgccgtacc agcggctagg
    24181 ccatcgacac catctgtcag attaacggca ttggtaaagc cgagaatgaa gaaaagaata
    24241 aaaggcaaat aaagtgagcc caattccagt gaataggtag taaaaggaat agtcacgtcg
    24301 gtagaggcgc ctagtgtgcg ccagcccaga taaaataaca ccactgtaat ggcctgcccc
    24361 aaaagtttat cgcgagcgcg caagccaagg gattgcttac gtactacctt taagtaatca
    24421 tcaataaaac cgattaaacc attaccaagc attaataata ttaccaggta caattcagta
    24481 cttgggggtg caaagagaaa ggtagccgga agacttacca ggagaaaaat caccccgccc
    24541 atcgtaggcg tacctgcttt ttttaagtgt ttacgtggtc cttcttcccg gatttgctgt
    24601 cccagtgcca aacgacgcat ttgacgaata taaaagggag ctaacactaa accaggcaaa
    24661 aaggcgctta ctgcggtaat taataaattt tgtttcatgc ttccaccctt tgcagcagct
    24721 tgagcacttc ttccatctgc ataccacggg agccttttaa taacagccaa tcccccgctt
    24781 gcgccaataa ctctacagct tcagctgcct gtacatgcgt tgtacactgc acgatcattt
    24841 ctgctgccat gcccgcctcc tgtgcgcctg cggcaatcca aggggaacga ggacccagca
    24901 ccactaaacc cttcagccgg ctggcagcag ccagtcggcc tacctggcga tggccttctt
    24961 cttccagcgc acctaactcc agcatatcac ctaatactgc aattttgccg tgctcaccgg
    25021 cctcttcccg caaaacattt aaagctgccg ccacagaagt gggactagca ttataagcat
    25081 cattaatcac ggtatagcct acaggcaaag ttgtaatttc taaacgcatg ccgcttaact
    25141 gcagctgcgc taaccccgcc tcaatttccg cattggttaa ttctaaagca cgccccacag
    25201 ctacagccgc caataaatta taaatattgt gccggcctgg cagtggtggg gtcaccacca
    25261 acggcggata acccgcctgt tctatttgta cttttttcgc tttgccgcca gttttacccg
    25321 ctgccgggcg aataactgct ttgcgagaaa aaccaaaggt tatcactggg caggccagct
    25381 ctcgccagta gggctctagc agcggctcat cagcattaac aactgccact ccatctgccg
    25441 gcaaggattg taacagttca catttggcct gggcaatacc ttcacgactg cccagcatct
    25501 ccaaatggga ttccccgata ttagtaatga caccaatgga cggtactgct agctgggcta
    25561 aagctgctat ttcacccaaa ccactcattc ccatttccaa gaccgccacc tctgtcttac
    25621 tggtcaggcg tgtcagcata attggcaggc caatttggtt attcagattg ccctcagttt
    25681 ttagtacttg gtaacggcct gagagagcag cagcaatcag gtcttttgtg gtggttttgc
    25741 ctacagaacc tgtgacggca actatcggca gagtatattg cagacgataa ccatgggcca
    25801 gttgttgtag tgctatcagg gtctccggta cggcaatcgc acatagttca gacggcgggg
    25861 taagttccgc agtttggtca acaaaacaac ctaccgcacc actgtccgca gcctgggcta
    25921 taaaacgatg gccgtcggta tgctccccgg cgaaaggcac aaaaaagtct cccgcctgca
    25981 caccccgtga atcaatgact acacgtttaa cttctgcctc ttggttggcc tccgtaacaa
    26041 ttttcccttg tacgagtgcc gcaatcgtct tacaattgat ctccattgtt cgttaatctc
    26101 ctctgcaacc atgctttgac aaccaaacga tcatcaaagg gaattgtgta attacggaaa
    26161 atttgctgtg tttcgtgtcc cttgcccgca ataatcacta cgtcgtcagt ctgggccatg
    26221 cttaaagcgc tttcgatcgc ctctttgcgg tcaggcagca ccaaatagtc acttttgttt
    26281 tccttttgta aaccaggtaa tatttcttta aagatctgcg tctgctcttc accgcggggg
    26341 ttatcagcag tgacaatgca gaaatcacta taaatgcctg ctatttctcc catcagcggc
    26401 cgcttgctgc ggtcacgttc accaccgcag ccaaaaacgg ttagcacacg cgcctgggca
    26461 aactcccgca ctgcacgcaa gatattttct aaaccatccg gggtatgggc ataatctacg
    26521 atgaccgtaa agtcctgccc ttcatctata acttcaaaac ggcctggcac accagtaatt
    26581 tcctccaaaa ccgctttgat atgccctaaa cccaaacctt ccaccaaggc tacagcaaca
    26641 gacgctagcg aattatagac agaaaacaat cctgtcattt ttaaattgag atcaagctgg
    26701 ccttctgggg tctctaaatg ataattaaca ccatcgcggg ttacctggat gtcagatgcc
    26761 cgcacgtgac aatgcgcaga caaaccataa agcagtactt ctcccggtgt ttgctctgcc
    26821 agctgccgcc agtacggatc atcgccattt aagatcgccc ggcgcatacg cttcttttta
    26881 accggcagcg agcctaacca agcaaacagt ttacttttac tggccagata atggtcaaaa
    26941 gtttggtgga aatctaaatg atcttcagta atattggtca gcactactgt atcgaagtca
    27001 cagccaattg tccgatacca agccaaggca tgcgaggaga cctccattgt cacatgcgtt
    27061 actcgtgcct taaccatctg acgtaaaagt gcctgcaagt cagaagcttc cggcgttgta
    27121 gccagcgagg gaaattcttg ccctcggata tggtaaccca ctgtgcccag cagacctgtg
    27181 gcggcaccgt cactttgcaa aagggcgtct atcagatgag tagtggtagt tttcccattg
    27241 gtgcccgtca caccggtaag gactaactct gtagcgggat aaccataata gcaggccgca
    27301 agtaaggcca aggctaagcg tgtgttcggc actgtaatga cggtcgcctg ttcagcagtg
    27361 aaaccgtgta catccgtgac gacggccacc gcaccggcgg ccagtgcctc tttaatataa
    27421 cgcttgccat gtgaacgtgt tccaggtaaa cagacgaaca aatctcccga ctggacacga
    27481 gccgaatgat aggaaatgcc cgtcaccggt cggtcgaggg ggccccttac ctctgcatgc
    27541 agcagagagc cgactagttc cgcaagcgtt ttcatcctgc tagtataccc ccttaattta
    27601 gttgtcacac ctacatttac caatctattt taataataaa taaatattca cttttcagag
    27661 aggtaatttg caacagcata agtcgtgcgc aaaaagggtg aagtgatctt caccctttat
    27721 cgccgctcgg catccggcgc agcaaacttc acgctaatga cactattagg tgccacttta
    27781 gtattggctg ccggttcttg ttctactgcc actccagaac cgctgctgtt taagcgcaac
    27841 cctaaccaac ctaaaacctc acccacttca cgcatcgttt tgcccgtaag ttcaggtaca
    27901 gtgatttcat tttcctcact agcaccaccc agatatacta agacctgggt atgcagtggt
    27961 acttttgccc ctgctttggg ggtctgattt aaaactgtag gaccttcgcc aatataacga
    28021 accaaaagcc cattgttttc taataacgca ttggtatggt catccatggc ctgcccgatt
    28081 aaattaggca cctctaccat actgggcaca gtttcctccg gcgttgtgtc aggagggata
    28141 ttcagatagt tcaagatact ctccatcatc cgtttaaaca tcggcgcaga aacctgggaa
    28201 ccccattgcg gtcctacctg cggcgcatca accgcgatat ataagagaat ttgggggtct
    28261 tccatgggca caaagccaat ataagaaaga atatattgac catcaacgta gcggccatta
    28321 ggacctactt tttgtgctgt accagtttta ccacccatgc ggtagccttc aatataagca
    28381 ttcatgccgc cgccctctgt aactaccaat tccataatcc gttttacttc ttccgaagtc
    28441 tcactggaga taaccctgcg tactacctgc ggctctttct ttgtgattat cgtaccctca
    28501 ctatcacgca tttccttaac aatatagggt ttcattaaat atccgccatt gatcatggct
    28561 gagacagcca tcacctgctg aataggtgtt acagatacac cttgcccgaa ggcggtagtt
    28621 gccgcttcca ccggcccata ttgtttaggt gaaaacaaaa tacctgttcc ttcaccaata
    28681 atatctacac ccgttttctg tccgaaacca aaggccttaa catagttcat taaggtatcg
    28741 gcaccaatcc gctgctgaag ttcgataaat ccagggttac aggagcctag cactacttcc
    28801 gtaaaatcaa tggcgccatg gccgccccgc ccccgcgtcc aacaaccaat ggaagcgcct
    28861 gccactttgg cataaccgct gcaaaagaac ccttcactag cgttatatcg gccttcctca
    28921 agagacgctg acaaggttac caacttaaaa gtagaaccag gttcgaaagt attagttaca
    28981 ggcgtcagct tccagttttc ctgtgcaaac tcaccataat tattagggtc aaaatctggt
    29041 ttaccagtga tccctaaaac cgcaccagtt ttaggattca cagcaatagc cacaatacca
    29101 tggggccgtg actccaacat cacccgatcc atttctcgct ccataataaa ctggatattt
    29161 tgatcaagtg tgagttccag cgtatagcca tccaccggtg gaataaatat ttctatgcct
    29221 tggggcacct gatgtccttt gccgtctgtt tgcatttcaa tccggccatc ctggcctttt
    29281 aattccgctt catactggtt ttccaaaccg gccaaacctt catcaagtcc cacaaaacct
    29341 agcaattgag aggctaagct accatgtggg taataacgtt tgggctccgg tgagaaatga
    29401 atgccgcgta tttccagctt gcgaatctct tgtgctactt gttcaggcat ttgtcgctta
    29461 agatacacag aatctgcttt ttgctttagt aattccagca gacgtccttc atccatctct
    29521 aaaattggtg ccagttttct tgctacttct tccttgtctt cctctttgat ctgattgggc
    29581 cgcgccataa tagacaatga actagcactg cccgctaaca acctagcgcg acagtcaaga
    29641 atgtcaccac gctgagcccg agccggatta gcacgtgtcc attgttccca agctttagac
    29701 tgcagctccg gtccccgtac cagttgtaac caagccaagc ggatgataag cgcagctacg
    29761 agagcgatca cacaaagaaa aaggaagagt agtctttttt tcacagttat attggatatc
    29821 gtatacctgc tcaaaagcta accctcccgg agtacttccc ctatcaagtt cggcaaagct
    29881 aagctgtacc ctaattttct gccgcctcag ctgtcagaat aatcatttgt tctctgccgg
    29941 gatattgcat accaagctca taggcttcgt tttccaagcg ctctagtgaa tttagagacg
    30001 ctattgacaa ttttaaatgc ttcccttctt cttgtaaagt ttgcaattcc gacctggcca
    30061 gctctacctg acgagctgct tcaactacct gtgtaaaatg cgaaattacg gcaatcgcca
    30121 ttacagcaca ggcaaaaatg gtcgcaaaaa gctttaattt ataggtagca gcgtgtaaat
    30181 taatctgacg tctattttgc gcgcgcgaca tgttccgttg cctacgagaa tgttgatatt
    30241 cctggggacg tttatgtgct actaacaact ttattcaccc tttcccttat ttagaacttt
    30301 gattgcggcg cgcaattttg cactgcgagc acgaggatta gctttaagtt cacgatcact
    30361 tgcctgcagg ggctttctag ttaaaatctt aatttgcggc tctttcccgc atacacacag
    30421 tggtaagccg ggggggcatt cacaaccagc agccgcatgc tggaacattt tcttcacaat
    30481 gcggtcttcc agggaatgaa acgaaattac tgccaagcgg ccacccggtt ttaagtaagt
    30541 gatgctctgc tcaagcgccc tttccaaaac ccccagttca tcattaacgg caatgcgtag
    30601 tgcctgaaag gtccgacgtg ccggatgggg gccacgacga cgcgcacgag ccggaatggc
    30661 ggccttaatg atgtctacca gttgagttgt tgtctcaatg gggccattgg ctacacgttt
    30721 agccacaata aagtcagcaa tccgcttcgc ccagagctcc tcaccgaaat cgcgaataat
    30781 gcgtgttaat tccgaagcat ctagataatt tattaaatcg gctgcagtcc tagtatcttc
    30841 tcgactcatc cgcatatcta aagcagcatt ggcatgataa gagaaacctc tttcatcttc
    30901 attcagctga tgactagaaa caccgatatc aagcaaaatt ccatctacag catctatacc
    30961 taactccgtg aggattgctt ccagttgtga aaaatttcgc tgcacaaagt gcacggagtc
    31021 agaccaagca gccagattct ttttagcggc gcttaaggct tcactatctt gatcgatggc
    31081 gactaagcga ccagccggca taattttctc taaaatagcg cggctatgcc caccaccgcc
    31141 taccgtacaa tccacgaaca cttcacctgg ttgtggattt aacacttgca tgacttcttg
    31201 cagcaagact ggttcatgtg agaaccgcat cagtaacact tccttacatt ccttttgttg
    31261 cctagtatcc ccctcacttt acagctctat atccataatt ttctctgcaa tactttcaaa
    31321 tgataaatcc gtttcctcac tataccgctg ccatacttcc tggctccaaa tctctacacg
    31381 actcgagacg cctattacca ccacatcttt ttgtaatttt gcatgattgc gcaaatttgt
    31441 ggggattaaa acgcgtccct gtttatctag ttcacattct gcagctccag aaaagaagaa
    31501 acgtgtaaag gcccgggcat cggagcgagt aaacgggaga tttttcaatt tttgttctaa
    31561 agcagtccat tcttgcagtg ggtacacgaa aaggcagttg tccaagccac gggtcagaac
    31621 aaaactatcg cctaattcct cacgaaattt agcaggcata atcaaacgcc cttttccatc
    31681 aactgtatgt tgatattcac ccataaacat ggacagccac ccccttcaag gtcatttccc
    31741 ccacttctct ccacttcata ccactttgac tacattcaac ggtacttctt attttcctgc
    31801 taataaaaaa aaaatttcaa agaaatttaa tctaacccgt gtcggccctt cttctctctt
    31861 tccgcaccgg cagtttagcg actactcccg catctattaa tttgcgcaaa aaaaaagacc
    31921 cgatgcctca ggtctttcac ctaaacttat tcaattgtca acttccctac actgtaagta
    31981 acgcataatc ttctttctta aggagatggg acgtaattac cctgtcacca catttgggac
    32041 acaaaatatc ttccacatta atttctgacc aactcactaa cccctgcaaa ccacatccca
    32101 gacacaggaa ctctacttta ccgtccaacg cctccatgag tcgcctcctt aaagtgtgtt
    32161 cagcaagtac caacctcagc tagtttattt attcaaattc gccgtaggcg ataaatttcc
    32221 tgttaatttt aaatcttttt actaataaaa atttgggcat cttttagttg ttactatcag
    32281 tgtatgcaaa aacaagtttt tctagaatac ttttgccaaa atattatgct ggtttgcaaa
    32341 taatctcttt aatgcgcgtg ttaaaaaaat ctttagcatg agccgttcgt aaaaccattg
    32401 ctgtatcagc accagcaccg gagcccccga tggccacaat gtcttcatca tgaggaatta
    32461 acccagcatc aagtgccatt accgcaattt ccacaccaac tttaacgcct tgtcccagca
    32521 tccgcagcgt ggctgccact aaacctccgg gataagtgcc gccaaacttg ttttctaccc
    32581 cgcggtcaat accaccaaac aaatgggtgg tcgtcagtac tttaaccccc ctggcttcca
    32641 gctttttacg cacttcagta tccatttcct gctccccagg gttacggaaa ccagtatggt
    32701 gcgtcacaca gaccacaggc actgccaagt ccagcagctg ctgcgctgtt ttaccactgt
    32761 tagaggctac cacatagtgg cggataccta attccctgcc ccgctcgacg gccagctgta
    32821 aggcctgttc cgtattcacc ggtcctcttt tcttaaagat cattacgtct cccccctact
    32881 tttttattaa tagtcttcac caatttttgt ctaacttatg taataattac cacacaaaca
    32941 ctaattaact aatatattta cttaacatct gcat
//
LOCUS       DASRMS010000178         3794 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_587671, whole genome shotgun sequence.
ACCESSION   DASRMS010000178 DASRMS010000000
VERSION     DASRMS010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3794)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3794)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3794
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587671"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1840
                     /gene="cooS"
                     /locus_tag="VFC74_07890"
     CDS             <1..1840
                     /gene="cooS"
                     /locus_tag="VFC74_07890"
                     /EC_number="1.2.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014253869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018492 - carbon-monoxide dehydrogenase
                     (acceptor) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic carbon-monoxide dehydrogenase
                     catalytic subunit"
                     /protein_id="HZK25293.1"
                     /translation="IRDDGLSSVFSRWQEQEKIRCKFCLQGMSCQLCTHGPCRISEKA
                     GAEKGVCGIGADAMAMRNFLMRNIMGAATYGHHAFEAFRTLKATGEGKTPFKITDVEK
                     LKWMCEQTGIETNQDTKQLALQLAELLDRELKVNPDQESIMVEAFAPKKRKAVWKELN
                     IYPTGVQHEVENCIASCLTNVDGDYVSLAKKAMRLGLSTIYTAQIGLEMTQDILFGTP
                     MPHEVDVDLGIMDPDYVNIAFNGHQPWLGVATLQKAKQDKYQEMAKKAGAKGLRIVGS
                     IETGQELLQRFEVDDVFVGLMGNWLAIEPFLATGTVDALVMEENCSPPAMDQYAEKYQ
                     VALISVSTIIGVPGTEHEMPYYPAKADEMADKCIEAAIENFKKRHGKIKPMVPQHKRK
                     AIAGFSTEAVLKAINNDLNALVEVIAKGQLKGIVALANCATLRNGPHDWNTVNITKEL
                     IKRDILVVAGGCGNHGLEVAGLCNLEAIELAGAGLQSVCKALGIPPVLSFGTCTDTGR
                     ISMLVTALADHLDVDISDLPIAVTAPEWMEQKATIDGIFAVAYGAYTHLSPTPFITGA
                     PNLVKLLTEDVEGMTGGKVALGDDPVQAAMDIEAHIIKKRAQLGLK"
     gene            complement(1919..>3794)
                     /gene="fdhF"
                     /locus_tag="VFC74_07895"
     CDS             complement(1919..>3794)
                     /gene="fdhF"
                     /locus_tag="VFC74_07895"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014969038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZK25294.1"
                     /translation="FINHSSRLTTPLIKKDGKLVKATWDEAYQLITKKVKEIKNKYGS
                     DVFAGLSSARYTNEENYLFQRLVRTAFGTNNIDHCARLCHSSTVVGLATTLGCGAMTN
                     SIGEIRDTDLMFVIGSNTTENHPVIANHMRQAQKKGAKLIVADPREIDLTKIADIYLQ
                     LKPGNNIALINGMMHEIIKNNWYDQKFVAEQTEGFDELVKHLQDFTPEKVAAVCGITA
                     EEIKKAAQLYAEADKAGIFYAMGITQHLSGVHATMALSNLAMLCGNIGKESAGINPLR
                     GQNNVQGASDMGCLPGDYPGYQKVYHAEMRKKFEQAWGVSLPDKIGLTLSEIIHGGGN
                     GTIKFLYITGENPMMTDPDISKVQQSLANLEFFVVQDIFLTETAALADVVLPASSFAE
                     KDGTFTNTERRIQRVRKAIEPLGDSKPDWVIFTELMARLGLPQNYQSPAEIMAEIASV
                     VPEYAGITYERLEEVGIQWPCLAADHPGTPILHKDKIDRGKGLFVTVDNISSAELADY
                     DYPFIMTTGRNLYQYHAMSMSGKVDELNNKSAESYIEINTATVARLGLNDGDQVKVSS
                     RRGSITTTVKATDKIGEEVMFMPMHFSDAKVNYLTNTAVDAMAKIPELKVAAVKVERM
                     "
BASE COUNT         1049 a          767 c          902 g         1076 t
ORIGIN      
        1 gatcagggat gatggtctat ctagtgtctt ctctagatgg caagagcagg agaaaatacg
       61 ctgtaagttt tgtctccaag gcatgagttg tcaattatgt acccatggtc cttgccggat
      121 cagcgaaaaa gctggagccg agaaaggggt ctgtgggatt ggcgccgatg ccatggccat
      181 gcgaaatttt ctcatgagga atattatggg cgctgcaacg tatggacatc atgcctttga
      241 agcctttagg acactgaaag ctacgggaga aggcaaaaca ccatttaaaa tcactgacgt
      301 tgagaaatta aagtggatgt gcgagcaaac aggtattgag acgaatcagg acacaaaaca
      361 actagccctg cagctggcag agttacttga tcgtgaattg aaagtaaatc cggatcaaga
      421 aagtatcatg gtcgaagctt ttgctccgaa gaaacgtaaa gcagtatgga aggaactgaa
      481 catatatcct acgggcgtac agcatgaggt ggaaaactgc attgccagtt gtctaaccaa
      541 tgtggatggc gattatgtct cccttgccaa aaaagcgatg cgtttgggtt tgtctactat
      601 ctatacagca caaatcggtt tagaaatgac gcaagatatt ttatttggta ctcccatgcc
      661 gcacgaagtt gacgtagatt taggcattat ggatcctgat tatgtaaata ttgcttttaa
      721 tggtcaccaa ccttggctgg gggtggctac actgcaaaaa gcaaagcagg ataagtacca
      781 ggaaatggcg aagaaggctg gtgccaaagg tctgcggatt gtcggttcca ttgaaacagg
      841 tcaggagttg ctgcagcgtt ttgaggtaga tgatgtgttt gtgggcttaa tgggcaactg
      901 gctagcgatt gaaccatttc ttgccacggg taccgttgac gctctagtga tggaagaaaa
      961 ctgctcaccg ccggcaatgg accaatatgc ggagaaatat caggtggccc tgattagcgt
     1021 tagtaccatt attggtgtgc cggggaccga acatgaaatg ccatactatc cggccaaggc
     1081 cgatgaaatg gctgataaat gtattgaggc ggccattgag aactttaaga aacggcatgg
     1141 caaaattaaa ccaatggtgc cgcagcataa gcgtaaagct attgccggat tttctacgga
     1201 agctgtattg aaggcgatta ataatgatct taatgccttg gttgaggtga tagcaaaagg
     1261 acagcttaaa ggtattgtgg ctttagctaa ctgtgcaaca ctgcgcaatg gtccccatga
     1321 ctggaataca gttaatatta ctaaagaact gattaaacga gatattttag tggttgctgg
     1381 cggctgtggg aaccatggtt tagaggtggc cggactttgc aatttagagg ccattgaact
     1441 agcgggtgca ggactacaga gtgtatgtaa agcattaggt attccacccg ttttaagctt
     1501 tggtacctgt acggatacag gaagaatctc gatgctggtc acagccttag ctgatcattt
     1561 agatgtcgat atttcagatt taccgattgc ggtgactgca ccagaatgga tggagcaaaa
     1621 agcaactatc gatggcattt ttgccgtagc ttacggtgcc tatactcatc tttctccaac
     1681 gccttttatt actggcgccc cgaatttagt gaaactgctt actgaagatg tggaggggat
     1741 gacaggtggt aaagtggcac taggtgatga ccctgtacaa gcagcgatgg atattgaagc
     1801 acacatcatt aagaaaagag cacagctggg tcttaaataa gcacagctga aataagatta
     1861 gtgtacaaag gaaaggccag gagcgtttag agcttcctgg cctttgcaat cttctttctt
     1921 acattctttc tactttcaca gcagcaactt ttagttctgg aatcttggcc atggcatcga
     1981 ctgccgtgtt ggtcagatag ttgactttgg catcggaaaa atgcatgggc ataaacataa
     2041 cttcttcacc aattttatct gttgctttga cggtagtggt gattgagcct cgtcgtgagc
     2101 taactttaac ctggtcacca tcatttaatc ccagacgagc aacagtagct gtattaatct
     2161 cgatataaga ttcagctgat ttattattaa gctcatccac tttaccgctc atggacatag
     2221 catgatattg gtagaggttt ctgccagtag tcatgatgaa gggataatca taatcagcta
     2281 attctgcact ggagatgtta tctaccgtca caaagagtcc tttgcctcgg tcaatcttat
     2341 ctttgtggag aattggtgtt cccggatgat cagcagctaa acaaggccac tgaataccta
     2401 cttcttctaa acgctcgtaa gtaataccgg catattcagg taccacgctg gctatttccg
     2461 ccatgatctc agcgggactt tgatagtttt gtggcagtcc taaacgtgcc attaattccg
     2521 tgaagattac ccaatccggc ttggagtcac caagcggttc aatggctttc cttactcttt
     2581 gaattctgcg ttcagtattg gtaaaggtgc catccttttc agcaaaggaa gaggcgggta
     2641 acactacatc agcaagagcg gctgtttctg ttaagaagat atcctgcacg acaaaaaatt
     2701 caaggtttgc taaagactgc tgtactttac taatgtcggg atcagtcatc atggggtttt
     2761 cacccgtgat gtataaaaac ttaattgtgc catttccccc gccatgaata atttcagaaa
     2821 gtgttaagcc tattttatcc ggtagactaa ctccccaggc ctgttcaaat ttcttacgca
     2881 tttcggcatg gtagactttc tgataaccgg ggtaatcccc gggcaggcag cccatatcac
     2941 tggcaccttg tacattgttt tgtccgcgta aaggattaat gccggctgat tctttaccaa
     3001 tattaccgca aagcatggct aaattagata aagccatcgt agcatggaca ccagagagat
     3061 gttgtgtaat tcccatcgcg taaaaaatgc cagccttatc tgcttcagcg tacagttggg
     3121 cagctttttt gatctcctca gctgttatgc cgcaaacagc agctaccttt tcaggagtaa
     3181 agtcttgtaa atgtttgacc aactcgtcaa acccttctgt ctgttcggca acaaactttt
     3241 ggtcatacca gttattcttg ataatttcat gcatcatgcc attaattaag gcaatattat
     3301 tgccgggttt aagctgtaga taaatatccg caattttggt aagatcaatt tccctggggt
     3361 cagcgacgat aagttttgcg cctttctttt gggcttgccg catatgattg gcaatcacgg
     3421 gatggttttc agtagtgttg gaaccaatca caaacattaa atcagtatca cggatttcgc
     3481 caatactatt tgtcatggca ccacagccta aggtggttgc aagtccgaca accgtagagg
     3541 agtggcagag ccgggcacag tgatcgatat tgttagtacc aaaggctgtt ctgaccaacc
     3601 tttggaagag atagttttct tcattagtgt atctggctga acttaagccg gcaaaaacat
     3661 cactaccata cttatttttt atttccttaa ctttttttgt aattagctgg taagcttcat
     3721 cccaagttgc tttgacaagt ttgccgtctt tcttgatcag gggagtggtt aatctagagg
     3781 agtgattaat aaac
//
LOCUS       DASRMS010000179        23219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_115282, whole genome shotgun sequence.
ACCESSION   DASRMS010000179 DASRMS010000000
VERSION     DASRMS010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 23219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23219
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115282"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(397..1215)
                     /locus_tag="VFC74_07900"
     CDS             complement(397..1215)
                     /locus_tag="VFC74_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015357776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HZK25295.1"
                     /translation="MKKFVYLLAVAVLSVSLLAGCGNTDSNDGDKKIIVGASPTPHAE
                     ILAVAKEVLAEEGYELQVQEFNDYILPNQALDSEDLDANYFQHQPYLDDFNAENNMDL
                     ATIAAIHYEPLGIYPGKTKVLADLPDGAEVLVPNDTSNEARALLLLEEQGLIKVKPDA
                     GLKATINDITENPKNLKITEIEAAQLARSLPDVDIAVINGNYAIQAGLNAATDALAKE
                     EKDSLAATTYANIIAIRKGDENREDLQALVNALKSDKVKKFIEDKYQGAVISVF"
     gene            complement(1235..1900)
                     /locus_tag="VFC74_07905"
     CDS             complement(1235..1900)
                     /locus_tag="VFC74_07905"
                     /EC_number="7.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013379289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033232 - ABC-type D-methionine
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0048473 - D-methionine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease"
                     /protein_id="HZK25296.1"
                     /translation="MGLLNPTITAMIVRGLGETLYMTLLSTLLAYLFGLPLGIALVVT
                     EKDGIRPQVRLNSILGLGINVLRSVPFLILLVAVIPITRFIVGKIIGSNATVVPLVIA
                     AAPFIARLVESSIKEVDKGVIEAAQSMGASPFQIIYKVMLPEALPSLIVGAAIAVTTI
                     LSYSAMAGFVGGGGLGTIAINYGFYRYQKGIMFLTVALLVIIVQVLQEIGTKLANLSD
                     KRN"
     gene            complement(1887..2897)
                     /locus_tag="VFC74_07910"
     CDS             complement(1887..2897)
                     /locus_tag="VFC74_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HZK25297.1"
                     /translation="MSSIIRLHGLSKSFTSANGEVVALENINLEIREGEVFGIIGMSG
                     AGKSTLVRCINFLEEPTSGTVYFAGRDLAQLSRPELYKVRQSMGMIFQQFNLLMQRTS
                     LGNVCFPLEIAGIGKIEARQRAEELLHLVGLADKAEAYPAQLSGGQRQRVAIARALAT
                     NPQVLLCDEATSALDPATTRGVLALLRDINKRLGITIVVITHEMSVIEEICQRVTIID
                     DGQIAETGTVEDIFSRPKSQAARRLVYPDDHKIEQINIGSRSIRIVFDGNSSFEPIIA
                     SMVLEFKHTVNILFADTKNIDGKAFGQLVIQLPEAEKVADKMIEYLRQKGLTVEEVDG
                     VA"
     regulatory      complement(3060..3159)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(3260..3835)
                     /locus_tag="VFC74_07915"
     CDS             complement(3260..3835)
                     /locus_tag="VFC74_07915"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HZK25298.1"
                     /translation="MRVTKDAQVRKQEIVETAMLLFEQNGIGKTSMSDIAQAAKITKG
                     LVYYYFSSKEELVTAVIELLATEINQQLQEIVQESALDFYGKLAEILKLYFVAIKERH
                     SLMEFSPANSGVYELVKNKLTESALLHTRDFVRQAKEAGILTIDYPEYTLSILINGIA
                     NLYVEGIQEPKILAVLIEQSLGLPKGSLKLG"
     gene            complement(3848..7360)
                     /locus_tag="VFC74_07920"
     CDS             complement(3848..7360)
                     /locus_tag="VFC74_07920"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZK25299.1"
                     /translation="MVMAKQSFYKELVRSIWQSRGRFFSVMAIIFLGVSFFAGINATE
                     PDMVLSADKYFKEQHLSDLRIFSPLGFAQQDLTALEQLSKVATVQKGYSKDLFFTTAA
                     GTTSVVKLMSYEPSNFTEQNGVNIPYLVAGRFPEKSGEILLVTGIGVPPELELDSTIT
                     VTVSDENGPLEYLTTAQFTVVGSIISPLYVTYERGQTNIGDGSVAYAAYALEKDFKLA
                     AYNEAYLLVQDTDKEQAYSTAYEHLIAPPQAAVEALGRQAVLRDTKELREKLEEGKQT
                     FLAEKQLAEEKLAAGQKKLDEAEEELINGEALLKEQEERYTKEIAAAKVELTVGKEAL
                     EQGKLKYFDGYTAWLEGYNQYQDGKAALLEAKAELDDAKVRLEAGERELAAARSELED
                     ARQQLVYLQDTLVVLREIEASLPDSDSELTEEEYRDLITRLEQVSPELARLIESSFDY
                     NDPNLINSLRSALQTAITELENQYETGQKQLLEGEQQLAAAEKTLQDNKKLYDEAWQA
                     YQPGVRELEASKVEIDQAKAKLERARQEIERNSAKLWQGERELLAAEAELQTSLATGI
                     QELEAGKIELAAGRETFAREKEEALLKIAEAEAEIKEAERQLLELPKEWFVYTRDGNP
                     GYAGYGDDAARVGAVAKIFPLFFFLVAALVCLTTMTRMIEEERMQIGTLKALGYGTWL
                     ISSKYLVYAFLASLVGSVLGFTIGFQLFPRVIMTIYGAMYDIPYLLSPFHLNYALISL
                     AIAISTTVVAALAASLATLQSTPAQLMQPKAPKPGKRIFLERLPFFWERLNFIQKVTV
                     RNIFRYKKRFFMTVLGIAGCTALLLTGYGIRDSVNVITEKQFDQIFLYDGMVIIDVEA
                     DAEAELAQILGTKREVDTYTDALMEVVTVSQEQGTHNYEANLMIPADERRLTDYFDLH
                     ERQTAAPLPLTKEGAVVTEKLAQLLDVAVGDVLVYQDTDNRTYQITIAGIAENYLAHH
                     IFMSREYFETVTLREPHYNAGIFTLHAADNFNDQQFRESLLNDDAVVGITLASIFQTN
                     FRDTLASLDYVVLILILAAGALAFVVLYNLTNINITERLREIATIKVLGFRDREVSSY
                     VYRENMILSFFGTLLGLVLGCILHKFVMETMEVDNIMFGKSIMPFSFLYAVLLTMFFS
                     ILVNFIMYFRLKQVDMVESLKSVE"
     gene            complement(7354..8055)
                     /locus_tag="VFC74_07925"
     CDS             complement(7354..8055)
                     /locus_tag="VFC74_07925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008982309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25300.1"
                     /translation="MAFVELHKVVKRYQMGEVTITAVDEVEFAIEKGDFAVVVGASGA
                     GKTTVLNLLGGMDKLDEGSIIVDGREISQYSKKMLTEYRRYDIGFVFQFYNLIQNLTA
                     KENVELATQISQNPLAVETVLALVGLQDRMNNFPSQLSGGEQQRVAIARALAKNPKLL
                     LCDEPTGALDYQTGKAILKLLQDTCRQRGMTVIIITHNSAIQGMADKIIKMQSGKVAE
                     FIVNEHPLPVEGIEW"
     gene            8351..9526
                     /locus_tag="VFC74_07930"
     CDS             8351..9526
                     /locus_tag="VFC74_07930"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK25301.1"
                     /translation="MTKSKSVITFAVMSVFFVAMGIGTITPAIQSIAEAFPDIAFSTL
                     LLVSTLPSLLIIPATLFSGAMAGTKIGYRPLLIVAMVLFIAGGVAPAFMNNFTTILVS
                     RAVFGIGIGLLSPLGNALVLGLFDGQQRANMLGLGGVIMNLGGIAFQMLGGLLAAISW
                     RYTFLAHGIAVASLLVVLFLLPEPKKMEVPAGPKATMPLAVYLISLLFGLAMMLNYPM
                     LVNMSTIIISGNLGTAASAGMVLSMFTVGGMLAGSIFGKLHQLVNRFTISVGLLITAL
                     GMALVYYANSLLVLTIGSTGVGIGFGLVMPAVMMVLGMIVPPHAFAIASGILMAVMNA
                     FAFLSTYYLEFIARITGAPAIKSPIFIAFCAYVAAAVIYTLARLKAPAAPSTADDAK"
     gene            complement(9738..10622)
                     /locus_tag="VFC74_07935"
     CDS             complement(9738..10622)
                     /locus_tag="VFC74_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK25302.1"
                     /translation="MNKEYQEHADLINVTANKRLSFRARLFSNKPATLAVVLIALFII
                     SALLAAFIAPYPYDEQNLPGMMQAPSIKHLFGTDEFGRDIFSRVIYGTRISLIVGFTS
                     VVISTSVGTLLGSLAGYYGGIVDQIITGLTDIVYSFPVSLLAIAVVAALGPSLFNLII
                     VISMVSWASYARLVRGQVLTLKEWDFVEAARALGMSNFRIIVRHILPNSLAPIIVMAT
                     LEIPKAIIIESTLSFLGLGVPPPTPSWGSIMNAGRSFILDAPWITMAPGVMMMVLVLT
                     FNLFGDALRDTLDPRLND"
     gene            complement(10641..11558)
                     /locus_tag="VFC74_07940"
     CDS             complement(10641..11558)
                     /locus_tag="VFC74_07940"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HZK25303.1"
                     /translation="MKKYFLKKLLFMIPVIIGSTVIIFLILHLSPGDPVDMIVGPNVT
                     PEVYQNIRISLGLDKPLVTQYFIFLGKLLRGDLGQSILQKRPVLDIVLERFPVTLKIG
                     VFSLILSFIIAVPMGIIAAIKRNTAADYMSMSFALLGISMPTFWFGLILLYFFAYKYR
                     IFPLSGYETWRHYVLPVITVGLTDAAVTARMMRSSMLEVIRQDYIRTARAKGVRETVV
                     MAKHALKNAILPVITLLGMRIGWIFAGSAVIEIVFSMPGIGRLMVDAIFARDYPIIQG
                     SMLILVISITLGNMLADFLYALVDPRIKY"
     gene            complement(11702..13279)
                     /locus_tag="VFC74_07945"
     CDS             complement(11702..13279)
                     /locus_tag="VFC74_07945"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK25304.1"
                     /translation="MESKKWRSLSLILVVLLTITLFLTGCGQETGDPDEEAAEKELVI
                     AYDRDAEILDTIKTSWLSDGLIYVYDRLVSRDYDFNYQPGLAASWDTSEDGLVWTFKL
                     KEGVKFHNGTPLKASDVKFTIESIIDPEVASPSASDLAAIKGVEVKDDLTFDIVLKHP
                     FPNLLFILSNTTCGIVSEEAYNNYGDEYGIKYVVGTGPYVFKEWIKGDKLVLEKNPEY
                     NWGPDWMENNGKPIIDSIIMRVIPEESTRRMELEAGNVHILRDVPASFVEELADNEEI
                     EVIRGDATKLGYLAYACDKEPFTDVRVRQAINHAINKNDIVTYLLRGVGQPAYGYLPP
                     ALKSEYYADSEKDGYAYDVEKAKALLTEAGYKDGLELTLSAENTSEYSKLAEVLQSQL
                     KEVGIETKIQLYDSSSYADMLKEGKQELFIRLYSWPNADILDFFLLSSQFPYPNHSRW
                     LDDTTDELIQKAAGSATWDERSAGYHEVQKHLIEQAVWAPIYIPDRVLAVRDEVQNFK
                     FHPWIPQYNDGFDLEVQ"
     gene            complement(13518..16040)
                     /locus_tag="VFC74_07950"
     CDS             complement(13518..16040)
                     /locus_tag="VFC74_07950"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HZK25305.1"
                     /translation="MKKILVIMLLMLLLVLPSDLIVSAQFSTLSCAQMFAEDGTVMLL
                     NEPQRNLLFTILSIIFLALLLLSTILWRNYRQLKTQNELISNLNQLRTTFIDADDRLI
                     YLKDEHLNYLFVNKAFEKFYQLKASEVIGCDDYFLSHQEFAQKRRDTDVAVIKKQSLL
                     VNEVVWQDKIYQTRKFPVHLESGNYGVGAYIQDVTEEYSNRMKAEKALQRNAILVEVL
                     SKDFYSTQEQLDYVLNELLKFTESKFGYIYLYDTQKQEFILNSWSKDVMSACTVMEKQ
                     TRYQLEATGLWGDVVRQRRPIMVNDYAGLTEMKQGYPEGHVQLTKFMSIPVIIEHEIV
                     AVVGVANKIADYDEHDIYQTTLLMQGVWNAKERRETQAELAVERKKYLLTLLSIGDGV
                     MVVDQAGKVEMFNEVAERLTGWSHKEAIGRHYQEVFVLSHELSGKVVNDPLAAALRTN
                     QVQQLENRAVLTAKNGQQYFIEDSAAPIIDDLKQTIGVVLVFRDVSEKKAQMKQIEFL
                     SFHDSLTGLYNRRFLEEEMCRLDTKRSLPIAILMGDVNGLKLANDIFGHTYGDLLLQR
                     VAEVLKKVCRAEDIIARWGGDEFVILLPKTNQQQAEQIKTRIKEEFTQEEIKAIKGSI
                     SIGCGFKLQEQESIFAALDSAEDDMYLEKTLTRQSTNDSLLRNIITSLHTMNPLEKAH
                     ASRVSELCVKIGQAMQLPADDLRKLNEAGYLHDIGKVALEVSLLNKHGVLQEQEWKEL
                     KQHPVVGFRILNSFENTMELAEYVLNHHEKLDGSGYPKGLKGPEIPKLARIIAIAESY
                     DAMTNATAYSRALSKEEALREIKQQLGSHFDPEIAAAFFLVV"
     gene            complement(16503..17756)
                     /locus_tag="VFC74_07955"
     CDS             complement(16503..17756)
                     /locus_tag="VFC74_07955"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZK25306.1"
                     /translation="MFVTNCKRNTTIMLIIVLSLLISKVAAAETTLPALAAEAAVLID
                     AQTGQVLFEKNMHQQKYPASITKVMTGLIALEKGEETDTLVISSEAVFSIEKNSAHIA
                     LDVAEEITLEQALYALSIPSANDAANGIAEYLAGDQGSFARLMTAKAAELGAKNTNFV
                     NAHGLEDAQHYTTAYDMAIIMMAAIKNPRFCEIFSETRYEILPTNIQPEIRYLHSTNA
                     LLNGLYPYEGIIASKSGWTSSAKHTLVTAAQRGSQKLIAVVLGCQNKDDRYLDTANLL
                     DYGFEQFKTVTFDSQELLKDTADLDEVKKIAPAGKISRLLHQSLEPQDIKKTISITNQ
                     TDNKLELQLTLTLKNASDLMYADLGKIPLSINLAGATKNQGFYYLPYFFAVLLLLAAA
                     LRIRAVRRRRRRQRLRLKKRYWGHV"
     gene            complement(18012..18653)
                     /locus_tag="VFC74_07960"
     CDS             complement(18012..18653)
                     /locus_tag="VFC74_07960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZK25307.1"
                     /translation="MKELFTIPNALFILAGLKMTLYISVVAIVLSTIFGTILGVLRSL
                     TGGIFRMAAALYIEVVRNIPNLLWIYVIFVVFRLKSIEAGVISFTIFTSAALGEIVRG
                     GLNSVGVGQWEAARSQGLNSIQVLRYVILPQALRNMIPAIVSQFVTVIKDTSFLWSVI
                     AIQELTGKVTILMGRYYKVSQVFALYLILALVYFVINYAISHFSRRLQQKLTY"
     gene            complement(18717..19460)
                     /locus_tag="VFC74_07965"
     CDS             complement(18717..19460)
                     /locus_tag="VFC74_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008428089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HZK25308.1"
                     /translation="MFSSTGPFAWFKWQALFADWQIFVQGFGYTLVVAISGLALALVI
                     GIIMGISSSSNVNPLKFIARCYVEFFQNTPLLIQVFFLYNGLPFAGIVLSVTTIGIIG
                     VGLYHGAYIAEVVRSGIGSIAKGQLEAAYSQGFTYWQAMTHIILPQAKTIILPPLTNQ
                     AVNLIKNTSVLAVISGADIMFTAKSWSSSNIYYGPAYVVAGFLYFILCYPLANLAKRL
                     EDKTNAMPTENKNKKGSGMTKPDLTVGSS"
     gene            complement(19646..20464)
                     /locus_tag="VFC74_07970"
     CDS             complement(19646..20464)
                     /locus_tag="VFC74_07970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003426629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HZK25309.1"
                     /translation="MKKTVAMLLMLVMLIALVGCSGGGNTGEQSGESEGDLPADLQAI
                     VNRGVLRVGTKVDVPGFGYQNPDTNEVEGMEIDIAKALAKKLLGDENKVETQGVTAQT
                     RGPLLDNNEVDVIIATFTITEERKLSYNFTQPYYTDAIGFLVKKAAGFKEIKDLDGKT
                     IGVAQSATTKDALEEKAKELGVTFKYGEYASYPELNAALASGRVDAFSVDKSILLGYV
                     NETSEILEEGFKPQEYGIASKLDNEDLAAYLEDFIAEIKADGTLDSIIGKWNLK"
     gene            complement(20503..21228)
                     /locus_tag="VFC74_07975"
     CDS             complement(20503..21228)
                     /locus_tag="VFC74_07975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014792895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HZK25310.1"
                     /translation="MIRMENVDKQFGKLLALKNINLEISSGEKLVVIGPSGSGKSTLI
                     RCMNYLEKPTSGKIFVDGVEITAHKAPIVKVRQSAAMVFQGFNLYPHKTVLENLTLAP
                     IKVQGIDKKQAEDSGLKYLERVGLSEKAHNYPSQLSGGQQQRVAIARALNMHPKIILF
                     DEPTSALDPEMIQEVLDVMVGLSKDNITMVVVTHEMGFARQVADRVIFMDEGEIVEQG
                     TPEHFFQNPAGERTKLFLSKILR"
     gene            complement(21726..22601)
                     /gene="hslO"
                     /locus_tag="VFC74_07980"
     CDS             complement(21726..22601)
                     /gene="hslO"
                     /locus_tag="VFC74_07980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013272907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HZK25311.1"
                     /translation="MSDYIIRATAAENQIRAFAATTRNLVEQARTIHHTTSVATAALG
                     RLLTAGSMMGAMLKSEKDVLTLQIKCDGPIKGVLVTADADANVKGYVHNPAVELPPQA
                     KGKLDVGKAVGSGILRVIKDLGLKEPYVGQTELVSGEIAEDITYYFTTSEQVPSAVAL
                     GVLIATDYTVKQAGGFIIQVMPGCDEEVIKSLELRISEMSSITSLLDAAMTPELILKE
                     LLGKFGLEMLAKIPTQFHCNCSKERVGKAIISIGRKDLQEIIAAKEPLEVNCHFCNTN
                     YQIELDDLQSLLNES"
     gene            complement(22993..>23219)
                     /locus_tag="VFC74_07985"
     CDS             complement(22993..>23219)
                     /locus_tag="VFC74_07985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003531746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2284 domain-containing protein"
                     /protein_id="HZK25312.1"
                     /translation="LKEKYSDFLMLTAGGCDVCKVCALIEDKPCRFPERAVSSVEAYC
                     IDVSLLARDCGMKYVNGQNTVTYFGAFLFN"
BASE COUNT         6492 a         5532 c         4368 g         6827 t
ORIGIN      
        1 tcctacagga tattaccagc attcattttt tattaacttt ttatatttat ccattatagt
       61 atcagcaaat atacggggta actatacgaa aacatcaatt ttctctcatc atgccagctg
      121 ctcgcttcaa caatcaggtt cagctaacaa tacctaacag ctattaattg aaaatgcagt
      181 atgctataat aaattgatag taaaatgtta ccaataaaaa ggagtggtaa aataagattt
      241 tgttggtgca caataaccga aacggtaata agcgttactt acattggata tgtgagaaag
      301 tattaaatct caataagtca aaaggaactg tgtcaagaca gttccttttt tttgtttctt
      361 ttgttttttt actccgaact gtgaagaccg tttttgttag aagacggaaa taacagctcc
      421 ttgatattta tcttcaatga attttttgac tttatcgctt tttagagcat tgacgagcgc
      481 ctgtaagtct tctctgtttt catcaccttt gcgtatggca attatgttgg cataagtggt
      541 ggcggccagg gaatcttttt cttcttttgc cagggcatca gttgcagcat ttaaaccagc
      601 ctgaatggca tagtttccgt taattactgc aatatctaca tcgggcaggg aacgagccag
      661 ctgcgctgct tcgatttcag taatttttag gtttttgggg ttctcagtaa tatcattaat
      721 tgttgctttt aaaccggcgt cgggcttaac tttaattagc ccctgctctt ccagcaaaag
      781 caatgctctg gcctcgtttg aggtgtcgtt gggcactaaa acttcagctc catctggtag
      841 atcagccaaa acttttgttt tgccgggata gatacctagc ggctcataat gaatggcggc
      901 aatggtggcc aaatccatat tgttttccgc attaaaatca tccaggtaag gctgatgttg
      961 gaagtagtta gcatcgagat cctcactatc caatgcttga ttgggcagta tataatcgtt
     1021 aaattcctgc acctgcagct cataaccttc ttctgctaaa acctctttag ccacagctaa
     1081 aatttccgca tgtggtgttg gtgaagcacc aacgataatc ttcttgtcgc catcgttgga
     1141 gtcggtattg ccgcagcctg ccagtaagga gactgacaat actgccacag ccaataaata
     1201 aacaaacttt ttcatctttt cccctccatt ttgtttagtt tcttttatcg cttagattgg
     1261 caagctttgt gccaatctcc tgcaacacct gaacgataat gactaggagt gccacagtca
     1321 aaaacataat gcctttttgg taacggtaga agccatagtt aatggcaatg gtgccaagcc
     1381 cgccaccgcc cacaaagccg gccatggcag agtaactgag aatggtagtc acagcaatgg
     1441 ctgcgccgac aattagggat ggcagtgctt ccggcagcat cactttataa ataatctgga
     1501 agggagaggc ccccatcgac tgtgcagcct caatcacacc cttgtccact tctttaattg
     1561 aggattccac taaacgggcg ataaagggtg ccgcagcaat gaccaaaggc actacggttg
     1621 cattagaacc aattattttt cccacaataa agcgcgtgat gggaattact gctaccagga
     1681 gaatcaagaa tggtacagaa cgcagcacat taatcccaag accgaggatg ctgttgagac
     1741 gaacctgagg gcgaataccg tccttctctg ttaccaccaa ggcaatgcct aagggcagac
     1801 caaaaagata ggctaacaac gtcgagagca gcgtcatata cagtgtctct cccagcccac
     1861 gcacaatcat cgcggttatc gtaggattaa gcaaccccat ccacctcctc cacggtaagt
     1921 cccttctgac gtaggtactc tatcatttta tcggcaactt tttctgcttc cggcagctga
     1981 attacaagtt ggccaaaagc cttgccatca atgttcttgg tatcggcaaa aagaatatta
     2041 acagtatgct tgaactccaa gaccatgcta gcaataatcg gttcaaaaga tgaattgccg
     2101 tcaaaaacaa tgcgaatact gcgactgcca atattaatct gctcgatctt gtgatcatca
     2161 ggataaacaa gccggcgtgc tgcctgcgat ttaggacggg aaaaaatatc ttctacagtg
     2221 cctgtttcag ctatctgacc atcatcaatg atggttacac gctgacagat ttcctcgatc
     2281 acactcattt cgtgagttat tacgacaatg gtaataccca gtcttttatt aatatctctc
     2341 aataaagcta gaacaccacg cgttgtggcc ggatctaggg cacttgtggc ctcgtcacag
     2401 agcaaaacct ggggattggt agctaaggca cgggcaatag ccacacgctg gcgctgtcct
     2461 ccagataatt gagccggata agcttcagct ttatcagcta aacctactaa atgcagcagc
     2521 tcctctgccc gttgcctagc ttcaattttg cctatacctg caatctctag cggaaaacaa
     2581 acattaccca aagaggtgcg ctgcatcagc aaattaaact gttgaaaaat catgcccatt
     2641 gattgcctta ccttgtacag ttccggacgc gagagctgcg ctaaatcacg gccggcaaaa
     2701 taaacagtgc cgcttgtcgg ctcttcgaga aagttgatac accgcactaa ggtgctctta
     2761 cctgcaccac tcatgccgat gataccaaaa acctcacctt ctctaatctc caaattaata
     2821 ttttcaagcg ctactacctc accgttagcg ctggtaaaac ttttgcttaa cccatgcaat
     2881 cggataatac tactcattac ctacccccca gtaatacaaa agctagatat agtacaaaaa
     2941 ttttactacg cagttttcgg tactgctgtg cagcggttac atttatcatc aggaagtatg
     3001 gcttgccagc tcgtacgaaa agcctcttcc cgacaagaag aggctttaaa atattactct
     3061 tcttatcttg caggaatctt cctacaggaa ttggcacctt ccaaaattgg ggttgccgtg
     3121 gtatcatcgg gccattccct ccaccactct tgataagatt taaatattaa atttttttct
     3181 atatgcagaa tatgatatca gtgattaaag tatctgtcaa acaatttttt accacccgct
     3241 tctcccgggt taatgctctc tagcccagct taagcgaacc tttaggcaga cctaacgatt
     3301 gctcaatgag cactgccaag attttaggtt cctggatacc ctccacataa agattggcga
     3361 tgccgttaat cagaatgctt aaagtgtatt caggataatc aatggttaag attcctgctt
     3421 ccttggcctg gcgcacaaaa tctctggtgt gcagcagtgc cgactctgtc agcttgtttt
     3481 taactaactc atacacaccg gaatttgcag gagaaaattc catcaagcta tggcgttctt
     3541 taatcgccac aaaatatagt ttaagaatct ccgctagctt gccgtagaaa tcaagagctg
     3601 attcctgaac aatttcctgc agctgctgat taatttccgt cgccaacagc tcaattacag
     3661 cggttactag ttcctcctta gaagaaaagt agtaatagac aagtccttta gtgatcttag
     3721 cagcttgagc aatatcactc attgaagttt tgccaatacc attttgttca aataacagca
     3781 tggccgtctc cactatttct tgtttgcgca cttgtgcgtc ttttgttact cgcataaatt
     3841 ttcgccccta ttccacagac tttaaggatt ccaccatgtc cacctgcttc agcctgaaat
     3901 acatgataaa attcaccagt atggaaaaaa acatagttaa caacacagcg taaaggaaac
     3961 tgaacggcat aatacttttg ccaaacatta tattatccac ttccattgtt tccataacga
     4021 atttatgcag gatgcagccc aggacaagcc ccagcagtgt accaaagaac gatagtatca
     4081 tattttcccg atacacatat gaggaaactt cgcggtcgcg aaaacccaac actttaatgg
     4141 tagcaatctc ccgcagccgt tctgtaatgt taatgttggt taggttatat agtaccacaa
     4201 aagccaaggc tccagcagct aaaatcagaa tcaacactac ataatctaaa ctagccaggg
     4261 tatcacggaa gttagtttgg aagatgctag caagagtaat accaactaca gcgtcatcat
     4321 tcagcagtga ttcgcgaaac tgctggtcgt taaagttatc cgcagcatgc agcgtaaaaa
     4381 ttcccgcgtt ataatggggc tcccttaaag tcactgtctc aaagtactct ctggacataa
     4441 agatatgatg ggctaaatag ttttcagcaa tccctgcgat ggtaatttga taagtgcggt
     4501 tatcagtatc ctggtacact aatacatcac caaccgccac atctaacagc tgggcaagtt
     4561 tttcagttac aaccgccccc tcttttgtca agggcagtgg ggcagcggtc tgccgttcat
     4621 gcagatcaaa ataatctgtg aggcggcgtt cgtccgcggg aatcatcaag ttagcttcat
     4681 aattatgagt cccctgttct tgagaaaccg tgaccacttc cattaaagca tcagtataag
     4741 tatcaacttc gcgttttgta cctagaattt gcgctaactc tgcttctgca tccgcctcta
     4801 cgtcaatgat taccatgccg tcataaagga aaatctgatc aaattgcttc tcggtgatca
     4861 catttactga atctctaata ccatagccag ttaataagag ggccgtgcag ccagcaatgc
     4921 cgagtaccgt cataaaaaat ctctttttat aacgaaagat gtttctgact gtcaccttct
     4981 ggatgaaatt taagcgctcc cagaaaaatg gcaggcgctc taggaaaata cgtttacccg
     5041 gtttgggcgc tttaggctgc atcaattgag ccggtgtcga ctgcagggta gccaagcttg
     5101 ctgctaaggc tgcgaccact gtcgtggaaa tcgcaatagc cagcgaaatt aaggcgtagt
     5161 taagatgaaa ggggcttaaa aggtagggga tatcatacat cgctccatag attgtcataa
     5221 ttacccgagg gaagagctgg aagccaatag taaagcccag cacactacca acaaggctgg
     5281 ctaaaaatgc atacaccaaa tatttagaag agatcagcca agtgccatag cctaaagctt
     5341 tcaaggtgcc aatttgcatc cgctcttcct caatcatgcg cgtcatagta gtcaagcaga
     5401 ctaaagcggc cactaaaaag aagaaaagag ggaatatttt agccactgca cctacccgtg
     5461 cagcatcatc accatagcca gcataaccag ggttaccgtc tctggtgtaa acgaaccatt
     5521 ctttaggcag ttccaaaagc tggcgttctg cttccttaat ttctgcctcg gcctcagcta
     5581 tttttagcag agcctcttcc ttttctcgcg caaaagtctc tctaccagca gccaattcta
     5641 ttttgcctgc ttccagttcc tgaattcctg tcgccagaga tgtctgtaat tcggcctcgg
     5701 ccgccagcag ctcccgctcc ccttgccaga gtttagctga atttcgctca atctcctggc
     5761 gggcacgttc aagtttagct ttcgcctgat ctatttccac cttactagct tctaattcac
     5821 gcacaccagg ttggtaggcc tgccaagcct catcatatag ttttttatta tcctgcagag
     5881 ttttttcagc ggctgccagc tgttgctcac cttccagcaa ttgtttctgg cctgtttcat
     5941 actggttttc cagttcagta atggccgtct gcaaggcaga gcgtaaggaa ttaattagat
     6001 tgggatcgtt ataatcaaaa ctactttcaa tcaatctcgc taattcaggc gaaacttgct
     6061 ccaaacgcgt tatcagatca cggtattctt cttccgtcag ctcactatca ctgtcgggca
     6121 gactggcttc aatttcccgg agcaccacca gggtatcctg caaatatacc agctgctgcc
     6181 tggcatcttc caactcgctg cgcgccgctg ccaactcccg ctccccggcc tccaaacgaa
     6241 ctttagcatc atcaagttcc gctttagcct ccagtaaggc cgctttacca tcctgatact
     6301 gattatagcc ttccagccaa gcggtataac catcaaaata ttttagtttc ccctgttcca
     6361 gcgcttcttt accaacagtt aactcgactt tagcggcggc aatttccttt gtataacgtt
     6421 cttcctgctc tttaagcagg gcttcaccat taattagttc ttcttcggcc tcgtccagct
     6481 ttttttgccc tgcggccagt ttttcttccg ctaactgttt ctctgcaaga aaagtctgct
     6541 tcccttcctc tagtttttca cgcagctctt ttgtgtctcg taacactgcc tgccgaccta
     6601 aagcctctac cgctgcctgt gggggagcta ttaagtgctc ataggccgta gagtaagctt
     6661 gctccttgtc tgtatcttgc accagcaaat aggcttcgtt gtaggcagct aacttaaaat
     6721 ccttttctaa agcataggca gcataagcca cggagccgtc accaatatta gtctgcccac
     6781 gctcataggt gacgtataaa ggagagatta tcgaacctac tacagtaaat tgagcagttg
     6841 tcaaatactc cagagggccg ttttcgtctg aaactgtcac ggtgatagta gaatcaagct
     6901 ccagctccgg tggcacgccg atgcccgtga ccagcaagat ttcaccgctt ttttccggaa
     6961 agcggcctgc caccagatag ggtatattaa caccattttg ctctgtaaag ttacttggct
     7021 catagctcat taatttaact acagaggtgg tcccggcagc tgtagtaaag aaaagatcct
     7081 tactgtagcc tttttgtact gtcgccactt ttgacagctg ctcgagagcg gtaaggtctt
     7141 gctgtgcaaa gcccaaaggt gagaagatac gcaaatcaga taaatgctgc tctttaaaat
     7201 atttatctgc tgaaagcacc atatccggtt ctgtcgcatt aatgcccgca aaaaagctca
     7261 cacctaaaaa aatgatggcc attacagaaa agaagcgacc acggctttgc cagatagagc
     7321 gcaccagttc cttgtaaaag gattgcttcg ccatcaccat tcaatccctt ctactggtag
     7381 tggatgctca ttaacaataa attccgcaac cttgccactt tgcatcttga tgattttatc
     7441 tgccatacct tgaattgcgg aattatgtgt aataataatc accgtcatgc ccctctgacg
     7501 acaggtatcc tgcaatagtt ttaaaatagc tttgcccgtc tgataatcta aagctcctgt
     7561 cggttcatca cacagcaata gtttagggtt cttggccagc gctctggcta ttgctacacg
     7621 ctgctgctct ccgcctgaca actgggaagg gaaattattc attctgtcct gcaaacccac
     7681 taaggctaag acagtttcta cagctaaagg gttttgacta atctgcgtcg ctaactccac
     7741 gttttccttg gctgtgaggt tttgaattag attgtaaaac tgaaacacaa aaccaatatc
     7801 atagcgtctg tactctgtca gcattttctt gctatattga gaaatttctc gtccgtcaac
     7861 aataatgctg ccttcatcta atttatccat gccccctaat aggttcagta ctgtagtttt
     7921 accggcacct gaagcgccaa caactacagc aaagtctccc ttttctatgg caaactctac
     7981 ctcatctaca gcagtaattg tcacttcacc catttgatat ctttttacta ccttatgtaa
     8041 ctcaacaaat gccataaata ataccacctt gtttttgcaa ttggtaactg tcaaacgcaa
     8101 accttttgtc tagttctatt ttactactga ctgacggtta gtcaataaga aatatttcac
     8161 gcagcgcatg aaaaagcata ttttttcata gtaatgacca ggactatcat gacagggaat
     8221 gtttattgaa tactcagact cctattgtag gtgatgtcga aatattctat aatgaacttg
     8281 agctggcgtt aaactctgaa ttgtcaccca gaagtgtaca caaaaaatta atctgggagg
     8341 tttaattact atgacaaaaa gcaaaagtgt cataacattt gctgtgatgt ccgtcttttt
     8401 tgtagccatg ggcattggta ctattacccc agcaatccaa agtattgcgg aagcctttcc
     8461 cgacatcgcc tttagtactt tactgctagt ttctacattg ccatcgcttt tgattattcc
     8521 cgcgacctta ttttccggtg ccatggcggg caccaaaatt ggctatcgtc ctttgctgat
     8581 tgtggccatg gttcttttta tcgctggcgg cgtcgcacct gccttcatga acaactttac
     8641 tactatttta gtgagcagag ccgttttcgg catcggcatt ggtctattat caccgcttgg
     8701 taatgctcta gtactcggtt tgtttgatgg tcagcagcgc gccaatatgt taggactagg
     8761 tggcgtcatc atgaatctcg gtggtattgc cttccaaatg ttaggcggct tactggcagc
     8821 cattagctgg cgttatacgt ttctggccca tggcatagct gtagcgtctt tacttgtcgt
     8881 gctctttctg ctacccgaac caaaaaaaat ggaagtacct gctggtccta aagctactat
     8941 gccgctagca gtttacctta tctctctgtt gtttggtctg gcgatgatgc ttaactaccc
     9001 catgcttgtg aatatgtcca caattattat aagtggtaat cttggcaccg cagcttctgc
     9061 cggcatggtg ctctcgatgt ttacagtagg tggcatgtta gctggttcca tcttcggtaa
     9121 gctgcaccag ctcgtcaata gatttactat ctctgtcggt ttgctcatca ctgctctggg
     9181 catggctctc gtttattatg ccaatagcct tttagtactt actatcggtt caaccggtgt
     9241 gggtattggt tttggcttgg tcatgccggc tgtaatgatg gttttaggca tgattgtacc
     9301 gccacatgct tttgccatcg cctcaggtat tctaatggct gtgatgaatg cctttgcctt
     9361 cctatcaact tactaccttg aatttattgc aagaattacc ggcgcaccgg ctattaaatc
     9421 ccctattttt attgctttct gtgcctatgt cgcagcagcc gtcatctata ccctggctag
     9481 actaaaagca ccagcagcac caagcaccgc cgatgatgcc aaataacagc atggcataaa
     9541 ctgccagtgc ataatcttgc gggacggcct tgaccgctcc actgcggcac aaataaactt
     9601 tcgttggcag gtgaatagta aactaatttt ctaatgacaa aaggtggcag gagctaactc
     9661 cctgccacct tttcttagcg ttgacaaaag atcaatgggt tagagcgaac gcagttcgtc
     9721 cacctgccac ctgaagatta atcatttaat ctcggatcaa gtgtatccct taaggcatcc
     9781 ccaaaaagat tgaaggttaa aacaagcacc atcatcatca caccaggagc catcgtaatc
     9841 cagggcgcat ctaaaataaa tgatcgtccg gcgttcataa tcgaacccca acttggtgtt
     9901 ggtggaggaa cacccaagcc cagaaaactt aaggttgact caataataat agcttttggt
     9961 atttctaaag tagccatcac aataatgggc gctaaagagt ttggcaggat atgtctcaca
    10021 atgatcctaa aattactcat ccccagtgcc cgcgcggcct ctacaaagtc ccattctttt
    10081 aaagttagca cttgccccct gaccaacctg gcataactgg cccaacttac catagagata
    10141 acaataatta aattaaaaag acttggacct aaagccgcga ccacggcaat cgccagcaaa
    10201 ctaacgggaa aagaatatac gatatctgtt aagcccgtga taatttggtc gacaatgccg
    10261 ccataatatc ctgctagtga accaagcaat gtccctacgc ttgtggagat cacgacagag
    10321 gtaaagccca ctattaaaga tattctggtt ccatagatca ccctgctaaa aatatccctg
    10381 ccaaattcgt ctgtaccgaa aaggtgtttt atactggggg cctgcatcat tccaggcagg
    10441 ttttgttcat cataggggta aggagcaatg aaggcagcaa ggagggcaga aataatgaaa
    10501 agggcaatca gcaccactgc caaggtggca ggtttattac tgaataacct agcccggaaa
    10561 ctcaatcttt tattagctgt aacattaatt aaatcggcat gttcttgata ttctttattc
    10621 acattttcac cctttactca ctaatattta attcgcggat caactaaagc atataaaaaa
    10681 tccgctaaca tattgcccag cgtaatggaa ataaccagaa ttaacataga accttgaatt
    10741 atggggtaat ctctggcgaa aatagcgtcc accattaatc tcccgatacc gggcatacta
    10801 aaaacaatct ctatgacagc cgaacccgca aagatccagc caattctcat ccctaataat
    10861 gtaatcacgg gaagaatagc attttttaag gcatgtttag ccattactac agtttctcga
    10921 acacctttag ctctggctgt acggatgtaa tcctgccgaa tcacttccaa catgctagag
    10981 cgcatcatcc tagcagtaac agctgcatca gttaacccta cagtgatgac cggcaaaaca
    11041 taatgacgcc aggtttcgta gccagagaga gggaaaatac gatatttgta ggcaaagaaa
    11101 tacagaagaa tcaaaccaaa ccagaaggtg ggcatggaaa tacccagtaa ggcaaaagac
    11161 atcgacatat aatcagcagc cgtatttctt ttgatagcgg caatgattcc cataggcacg
    11221 gcgataataa atgataagat tagagaaaaa acaccaattt ttaaggtgac aggaaagcgt
    11281 tccagcacaa tatccaacac aggccttttt tgcaatatcg attggcctaa atcgcctctt
    11341 aataattttc ccagaaaaat aaaatattgc gtaacaaggg gtttatccaa gcccaggctt
    11401 attctaatat tttgataaac ttctggcgtg acattggggc caacgatcat atcgactgga
    11461 tctcctggtg ataggtgcag aattaaaaaa atgatcacag ttgagccgat aataaccgga
    11521 atcataaata acagcttctt caaaaaatat tttttcatat ctcacccctg ttttcattaa
    11581 gggcatgcag cgatctatca ataacagatc gctgcatgct tgtttctttt attggcttgc
    11641 ttggcggcta atagtagaag tttgtctaaa aaacatctgt ctgcgtcttt cgctcatgaa
    11701 gctattgaac ttctaaatca aagccatcat tatattgcgg tatccaggga tggaatttaa
    11761 agttttgtac ttcatctctc accgctaaga ccctatctgg aatgtaaata ggtgcccata
    11821 cagcttgctc aattaagtgt ttttgcactt catgataacc tgcagatcgc tcatcccaag
    11881 ttgcagagcc cgctgctttt tggattaatt catctgtcgt atcatcaagc catctagaat
    11941 ggtttggata ggggaactgg ctcgacaaca agaaaaagtc tagaatatca gcgttgggcc
    12001 aactatacag tctaataaac agttcttgtt taccttcttt taacatatcc gcataacttg
    12061 agctatcata tagttgaatt ttagtttcaa tgcctacttc ttttaattgg ctttgcagca
    12121 cttctgccaa tttgctgtat tcactggtat tctctgctga taaagttaat tctaggccat
    12181 ctttatagcc tgcttcggtc aggagagcct tagctttttc tacatcatac gcataaccgt
    12241 ctttttcact gtcagcatag tattcactct ttagagcggg tggcagataa ccataggcag
    12301 gctgtcctac accgcgcagc agataggtga caatatcatt cttattgata gcatggttaa
    12361 tcgcttgccg tactcgcaca tctgtaaatg gctctttatc acaagcataa gcaagataac
    12421 ccagcttggt ggcatcgccg cgaatcactt ctatttcttc attatctgca agttcttcca
    12481 caaagctggc aggtacatct cttaaaatgt gtacattgcc cgcctctaat tccatgcgcc
    12541 gcgtgctttc ctcggggatc actctcatga taatgctatc gataatcggc ttaccgttgt
    12601 tttccatcca gtcaggaccc caattatatt cggggttttt ctcaagtacc agcttgtcac
    12661 ctttaatcca ttctttaaat acatagggac ctgtaccaac tacatactta atgccgtact
    12721 catcaccata gttgttgtag gcttcctcac taacaatacc acacgtggtg ttagagagaa
    12781 taaataaaag attggggaag gggtgcttca aaacaatgtc aaaggtcaaa tcatccttta
    12841 cctctacacc tttaatcgcc gctaaatcac tggccgatgg tgaagctacc tcaggatcaa
    12901 taatactttc aattgtaaac ttcacatcac tagcttttaa aggtgtgcca ttatgaaact
    12961 tgacgccttc tttaagcttg aaggtccaaa ctaaaccgtc ctcggaagta tcccatgagg
    13021 cggccaggcc tggctgataa ttaaaatcgt agtctctact taccaaacga tcataaacgt
    13081 aaatcagccc atctgataac cagcttgtct taattgtatc tagtatttct gcatccctat
    13141 cataagcaat tactaattct ttttctgctg cttcttcatc tggatcacct gtctcttgtc
    13201 cacagcccgt caagaaaaga gtaatcgtaa gtagtactac tagtattaaa ctcaagcttc
    13261 tccatttttt actttccatt agttacctcc tacttttatt ttttctatat aaaggataac
    13321 caccccctga agtaagtggt tatctttata agctttctga aaatactaca aattatccta
    13381 ctatctaaat tttaacaatg tttattttat ctgtcaaccc tcctagactt tgtattaaac
    13441 tactgcctgc ctgccaaata tctcaaaata ccccattaat catccgctga cacgtccgaa
    13501 gtaaggggca actacaccta cactactaaa aaaaatgcgg cagcaatctc agggtcaaaa
    13561 tgagagccca gctgttgttt aatttctcta agtgcttctt ctttgctcag cgcacggcta
    13621 taagctgtcg cattagtcat agcatcataa ctttcggcaa tagcaattat tctagcaagc
    13681 ttagggatct ctggtccctt taaacctttg ggataaccgg aaccatctaa cttttcgtga
    13741 tgattaagta catactctgc caattccatg gtattttcaa aagagttgag gattctaaaa
    13801 ccaactacgg gatgctgctt tagttctttc cactcctgct cctgcagtac cccatgctta
    13861 ttaagcaaac taacctccag ggcaacttta ccaatatcat gtaaatagcc agcctcattt
    13921 agcttacgga gatcatcagc aggaagctgc atagcctggc caattttaac acaaagttca
    13981 ctaaccctgc tggcgtgagc tttctccaaa gggttcatgg tatgcagact ggtgatgata
    14041 ttccgcaata atgagtcatt agtgctttga cgagttagtg ttttttctaa atacatatca
    14101 tcctctgcgc tatctaaagc cgcaaaaatg ctttcctgct cctgaagttt aaatccacag
    14161 cctatggaga tgctgccttt tattgccttt atttcttctt gagtaaactc ttcttttatc
    14221 cgtgttttaa tctgttcggc ttgctgttga tttgtcttag gcaataaaat aacaaactca
    14281 tcgccgcccc agcgagcaat gatatcctcg gctcgacaca cttttttcaa cacctcagct
    14341 actcgctgca gcagcaaatc cccataggta tgaccaaaaa tatcattagc taattttagc
    14401 ccattaacat cacccatgag gatagctatc ggcaaacttc tttttgtatc gagtctacac
    14461 atttcctcct ctaaaaagcg acgattatac aatccagtta gtgaatcatg aaagcttaaa
    14521 aattctattt gcttcatctg tgctttcttc tcactaacat ctctaaacac taaaaccaca
    14581 ccaatagttt gcttaagatc atctataatt ggggctgcgc tatcctctat aaaatattgc
    14641 tgcccatttt ttgcggtaag cacagcacga ttttctaatt gctgcacctg atttgtgcgc
    14701 aatgctgcgg ccaggggatc gttaaccact ttcccagaca gctcgtgaga aaggacaaat
    14761 acttcttgat aatggcgacc gatcgcttct ttgtgcgacc agcctgttag cctttctgcc
    14821 acctcattaa acatttcaac tttccccgcc tgatctacca ccattacccc atcaccgatg
    14881 gacagcaaag taagcaaata ttttttcctt tccacggcta attctgcctg cgtttctctt
    14941 ctttctttag cattccagac accttgcatc aggagagttg tttgatagat atcgtgctca
    15001 tcatagtcag cgattttatt agcgactcct accactgcaa caatttcatg ctcaatgatg
    15061 acaggaatag acataaattt agttaactgg acatgtccct cgggatagcc ctgcttcatc
    15121 tcagtcagac cggcatagtc attaaccata attggtctgc gctggcgcac gacatctccc
    15181 cacagccccg tcgcctccag ctggtaacgc gtttgctttt ccatcaccgt acaagcagac
    15241 atcacgtcct tggaccagga atttaaaata aactcttgtt tttgagtatc ataaagataa
    15301 atataaccga acttgctttc cgtaaacttc aataattcat ttaagacata atccagctgt
    15361 tcttgcgtag aataaaagtc tttacttaaa acctccacca gaattgcatt gcgctgcagc
    15421 gctttttcag ccttcatgcg gttactgtat tcctcggtaa catcctggat ataagcaccg
    15481 acaccatagt ttccgctctc aaggtgcacg ggaaattttc ttgtttgata tattttgtct
    15541 tgccaaacaa cttcgtttac cagcagactt tgctttttta tgacagccac atcagtatca
    15601 cgacgtttct gcgcaaattc ttgatgagag agaaagtaat catcacagcc aataacctcc
    15661 gaggctttaa gctgataaaa cttctcaaaa gctttattaa caaagagata gtttaaatgc
    15721 tcatctttta aataaatcag cctgtcgtca gcatcaataa aagttgtacg taattgattg
    15781 agattgctaa ttagttcatt ttgtgttttt aattgccggt aattgcgcca taagatagta
    15841 cttaacaaca gcaaagccaa gaagatgata cttaatatcg taaaaagcaa gtttctttgt
    15901 ggctcgttca gcagcattac agtaccatct tcggcaaaca tttgtgcgca actcagtgtt
    15961 gagaattgcg ctgaaacaat taaatcacta ggcagcacaa gcagaagcat taacagcatg
    16021 atcaccagta tctttttcat ttaccttcca acccctattc tgcttacgcc tgcttattgt
    16081 tttcgtattc taacactttt tgcacaaaat attggacgat tacgggagca aactgactgt
    16141 ctgcataatt atgcaatttg gcagctattg tcaacactaa agcacacaaa aaaggccaca
    16201 atcaaattgc gccatgacag aaatttcgtc aagcagctgt tcattatctt ctgcgcaaga
    16261 ccatgagatc cttacataaa tattatgcca tctgacagtc aagagtgtta acaaaaacta
    16321 atattaagac acatttcgcc atatattctg tttagatcct ctacatcaac cataattttc
    16381 tcctacctac cccatgctcc atcaatatca taatcttgat ctgatcggct gattgaaagt
    16441 tataaagagg gccatggatg tttacccacg gccctctcct cgttatcatg cctatactag
    16501 cttcatacgt gaccccaata cctttttttc aacctcaacc gctgtcgtct gcgtcgccgc
    16561 cgaacggcac ggatgcgtaa ggctgctgcc agcaggagca ggacagcgaa aaagtagggc
    16621 agataataaa acccctgatt ctttgtagcg cccgctaagt tgatgctcaa gggaatcttc
    16681 cctaaatctg catacattaa atcactagcg tttttcaggg ttagggttag ttgtaattct
    16741 agcttgttgt ccgtttggtt ggtgatagag atagtttttt taatatcttg cggttctaaa
    16801 gactgatgca gcagccttga tattttgcct gctggggcga tttttttcac ctcgtctaaa
    16861 tcggcggtgt cttttagcag ctcctggcta tcaaaagtga ccgtcttgaa ctgttcgaaa
    16921 ccatagtcta ataaattagc tgtatcaaga tagcggtcat ctttattttg gcaacccaaa
    16981 acaacagcta ttagcttttg gctgccacgc tgggcagccg ttaccaaagt gtgttttgct
    17041 gatgaggtcc agccgctttt actggcaata atgccttcgt agggatagag tccattcagc
    17101 aaggcatttg tactgtgtaa atatcttatc tctggctgta tattagtcgg cagtatttcg
    17161 taacgagttt cactaaatat ttcacaaaaa cgtgggtttt taatggctgc catcataatt
    17221 atggccatat cataggctgt agtatagtgc tgagcatctt ccagaccatg ggcgttaaca
    17281 aagtttgtat ttttggctcc taactcggca gccttggccg tcatcagccg tgcgaaactt
    17341 ccttgatcac cggcaagata ctcggcaata ccattggctg catcattggc tgaggggatg
    17401 gacagagcat aaagcgcctg ttctaaggtg atttcttcag caacatcaag agcaatatga
    17461 gcggagtttt tttcaataga aaatactgct tcgctgctga tgactagtgt atcagtctcc
    17521 tcaccttttt ctaaggcgat cagacctgtc atcaccttgg taatggaagc aggatacttt
    17581 tgttggtgca tattcttctc aaacagcact tgtcccgttt gtgcatcaat taaaacagcc
    17641 gcttcagctg ccaatgctgg taatgtagtt tcagccgctg ccaccttact tattagtaaa
    17701 ctcaagacta tgatgagcat aattgtggta tttcttttac aattagttac aaacatctct
    17761 tcttctccgt tgtcgttatt tatctttttt ctttacttta tatcataacg ctgtactctt
    17821 atcatgtcca ctgttggtaa cgaattactt tatagtaata cttggttaat tggcacagca
    17881 aaaaacaata gagcacgcct gcaggattga gttggcaggc aaatttgctc tggagcaaga
    17941 aagtaaaagg ctgctgttag ccagcagccc ctaattgcca taacctttta gtaatagctc
    18001 gtactaatat tttaataagt taatttttgc tgcagcctcc gggaaaaatg tgatatcgca
    18061 tagttaataa caaagtagac taaagcaaga attagataga gggcaaatac ttgactaacc
    18121 ttatagtaac gtcccatgag gatagtcact ttacccgtca gttcctgaat agcaatcact
    18181 gaccaaagaa aactggtatc cttaatcact gtcacgaact gtgaaacaat agctggaatc
    18241 atattgcgga gagcttgtgg taaaatcaca taccgcagca cctgaatact attaagacct
    18301 tgtgaacgag ccgcttccca ctggcctaca ccgactgagt taaggccacc acgtacaatt
    18361 tcacccaatg cagccgaggt aaaaatagta aaactaatca ccccagcctc aatagattta
    18421 agtctgaaga caacaaaaat cacgtagatc caaagcagat taggtatatt acgcactacc
    18481 tcaatatata atgcagctgc catgcgaaaa attccgccgg ttaaactgcg tagtacaccc
    18541 agaatggtac cgaatatggt actcagcact atagctacca cagaaatata taaggtcatt
    18601 ttcagtcctg cgagaatgaa aagagcatta ggtatggtaa atagttcttt cataaggacc
    18661 tcacttctgc gccagtctaa aaatgtctac agacggtgct cgctcactgg cgcccatcag
    18721 gagctcccga ctgttaaatc tggtttagtc atgcctgacc cctttttatt tttgttctca
    18781 gtgggcatgg catttgtttt gtcctccagc ctttttgcca ggttagccag ggggtaacac
    18841 agtataaaat agagaaatcc tgccacgaca taagctggcc cataataaat gttagatgat
    18901 gaccatgatt ttgccgtaaa cataatatca gcaccagaaa taactgccaa aacagaggtg
    18961 tttttaatca gattaactgc ttgatttgtc aatggtggca gaatgattgt ttttgcctgc
    19021 ggcaggatga tatgcgtcat cgcctgccag taggtgaaac cctgagaata agcagcttcc
    19081 aactgacctt tagcaataga gccaatccct gaacgtacaa cttcagcaat ataggcgcca
    19141 tggtacaatc ctacgccaat aatcccgata gttgttacgc ttaaaacaat gcctgcaaat
    19201 ggtaggccgt tataaagaaa gaacacttga attaataaag gtgtattctg aaaaaattca
    19261 acatagcatc tagcgataaa tttaagaggg ttaacgttag acgaggaaga gatacccatg
    19321 ataattccaa tcacgagcgc cagcgccagc ccacttatcg ccactactaa agtatagcca
    19381 aaaccctgca caaagatctg ccagtcggca aacaaggctt gccacttaaa ccatgcaaaa
    19441 ggtccagtac ttgaaaacat gccgtatcgt cctttcaaaa gctatgcgcc gcgcttgatt
    19501 ttgcacgaga cttacagcca gcattctggc acaaaagagt accgcctctt tactagtttc
    19561 aaggcgtgaa gcgcctgaag ggttcgggag gcggtgcttc ttgatgccgt ttttggtgtc
    19621 ttagtttagc tggaatttaa ctaatttatt ttagattcca tttgccaatg atactatcca
    19681 aagtaccatc cgctttaatc tcagcaataa aatcttcgag atacgccgct aaatcttcgt
    19741 tgtctaactt gctggcaata ccatattcct ggggtttgaa gccttcttct aaaatttctg
    19801 aggtttcgtt tacataaccc aagagaattg atttatcaac actgaaggca tcaacccgtc
    19861 cggacgccag tgcggcgttg agttcaggat aacttgcata ttcgccatat ttaaatgtta
    19921 cacccaactc tttagctttt tcttccagag catccttggt agtagcagat tgtgctacac
    19981 caatggtttt accatcaaga tctttaattt ctttaaagcc tgctgctttc ttgactaaaa
    20041 aaccaatagc atctgtgtag tatggttgcg taaagttata agataactta cgttcttctg
    20101 tgatggtgaa ggtggcgatg atcacatcta cttcattatt gtccagtaaa gggccgcgtg
    20161 tttgagcggt aacgccttgt gtctctactt tgttttcatc acccagcaac tttttagcaa
    20221 gtgctttggc aatatcgatt tccatgcctt ccacttcgtt agtatccgga ttttgataac
    20281 caaagccggg tacatccacc tttgtaccaa cacgcagcac gccgcgattg acgatcgctt
    20341 gtagatctgc aggtagatcc ccctcagatt ctcccgactg ctccccagta ttgccgccgc
    20401 cagaacaacc aactaaagca ataagcatta ccaacataag caacatcgct actgtctttt
    20461 tcatttttta cccttccctt cccttccaat tttccctctt gtttatctta gtatcttact
    20521 taagaacaac tttgtcctct caccagcagg gttttggaaa aagtgttcag gcgtaccctg
    20581 ctccacgatt tcaccctcat ccatgaaaat aacccgatca gcaacctgtc tagcaaagcc
    20641 catttcatgc gtcactacta ccatggtgat attatcttta gaaagtccta ccattacgtc
    20701 cagaacctcc tgaatcatct cagggtctaa agctgaagtt ggctcgtcaa acaagataat
    20761 tttcggatgc atattcaaag ctctggcaat ggccacacgc tgctgttgtc cacctgaaag
    20821 ttgtgaagga tagttgtgag ccttttctga aagccccact ctctccaaat acttcaaacc
    20881 tgaatcttcg gcctgctttt tatctatgcc ctgcacttta atgggtgcca gtgtcaggtt
    20941 ctctaggacc gttttgtggg ggtaaagatt aaatccttgg aataccatgg ccgcagattg
    21001 cctcacttta acaattgggg ccttatgtgc ggtaatttcc accccatcca caaaaatttt
    21061 acctgatgta ggtttttcca gataattcat acatcttatt agagtacttt tcccagaacc
    21121 agatggccca ataacaacta atttttcgcc tgagcttatc tctaaattta tgttctttag
    21181 tgccaaaagt ttaccaaatt gtttgtcgac attttccatt cttatcaata aaatcagtcc
    21241 ttatctttta catgcttttg ctaatatcct tttctgatcc tgtagcgctg gcgaccgtga
    21301 ccaccccgca agatttaagg gcaaccccct caaaaccccc gccaggaagg aaagtcagcc
    21361 aatcatggtt gatagattgt gcgctttgat aaaaaagatg gccttaaaac gccatagttg
    21421 ctagatgcac aatttataat cttttctgaa ttataattgt taaccattat aacagatgtt
    21481 tttttccttg tctataacct atccaatgta aagcattggc aattatttat ttgtcctgcc
    21541 atgttaataa cagatgttat aaccacctaa ccttcaattt actcattcat gcttatccat
    21601 tcaactaaca agcaaaaaac aagaaagaca cccctgcttt tttacagaag cgtcttcctt
    21661 gtgctttggg ctgagttaag tgggccttcg tccctatcaa aatcacagtc ttatctgctt
    21721 tttagttaag actcgttcag caagctttgt aaatcatcaa gctcgatttg gtagttagta
    21781 ttgcagaaat ggcaatttac ttctaaaggc tctttagcag caatgatctc ctggagatcc
    21841 tttctgccaa tactaataat tgctttcccc acacgttctt tactgcaatt acagtgaaat
    21901 tgcgtcggta ttttagctag catttctagg ccaaatttcc ccagcaactc tttcaatatc
    21961 agctctggcg tcatcgctgc gtctaaaaga ctagttatgc tcgacatttc actgatcctt
    22021 aactctagcg acttaattac ttcttcatca caccctggca tcacctggat gataaagccc
    22081 ccagcttgct tgacagtata atccgtagct attagcactc ctaaagctac ggcagacggt
    22141 acttgttcag aagtggtaaa gtaataagta atatcctcag caatttcacc agaaactaac
    22201 tctgtttgtc ctacataggg ttctttcaat cccaagtctt taattactct taaaattcct
    22261 gaccccacag ccttccccac atccagttta cctttcgctt gaggaggtag ttccacagct
    22321 ggattatgaa catagccctt gacattagca tcggcatctg ctgttaccag cactcctttt
    22381 attggtccat cacacttaat ctgtaacgtc aaaacatctt tttcactttt taacatggca
    22441 cccatcatgc ttcctgctgt cagcaaccgg cctaaagcag cagttgccac tgaagtagta
    22501 tgatgaatag tccgcgcctg ctccaccaaa ttacgtgtgg tggcagcaaa agctcgaatc
    22561 tgattttccg cagctgttgc tctaattata taatctgaca tctttcctgt acttgatcta
    22621 cattgcttaa ccctggcaag cagttaacct cggccaagga tctctttcgc gagtagacaa
    22681 gtaacagtta cacctcctta agttagcttc taccctatct ttatctgaag atgagcaagt
    22741 tatttcgtta ctaaaaaagg cctcttcagc ggttctaacg ttacaaatta taagatgccc
    22801 tgcggctagt gtcaaatagt gccgctaatc taaactaact gcagggaaat ttaagccaat
    22861 ttttctgtac acaccgcggc atgcctacgc cgaaccataa tatttgtcag ccaagataag
    22921 aaagcaagaa gctgcttgac aagagcttct tgctttcttt gtttataaac ttttcctgtc
    22981 accctagcag ctttagttga ataaaaaggc gccaaaataa gtcacagtat tttgcccatt
    23041 gacatatttc atgccgcaat ctctggccaa aagggaaaca tcaatgcagt aggcctccac
    23101 agaggaaaca gcccgttctg ggaacctaca aggtttatct tcaattaaag cacaaacctt
    23161 acagacatca cagcctcccg ctgtaagcat caaaaaatca ctatattttt cttttaatt
//
LOCUS       DASRMS010000180         1239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_864892, whole genome shotgun sequence.
ACCESSION   DASRMS010000180 DASRMS010000000
VERSION     DASRMS010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_864892"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..919
                     /gene="istB"
                     /locus_tag="VFC74_07990"
     CDS             197..919
                     /gene="istB"
                     /locus_tag="VFC74_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HZK25313.1"
                     /translation="MSKALISELCRQLRLGTYIADSYTEVEADSHEEFLIKLLTEAVA
                     KRSTERRKRYIQQAGFDLMKGYDDFDFSDLVLPAGLTPESLRTCDFVEMQQNLILYGR
                     PGTGKTHLAIALGIEACKRDYKVLYCKTSRLVNLLSQAKEEISESKFWRKLNTADLLI
                     LDEWGYIPFERVGTQLLFEAISDCYEKRSVIITTNLPFDEWNTIFYDQKLTAAILDRL
                     IHHGLLLMHDGQSYRLQNSQMQ"
BASE COUNT          349 a          266 c          289 g          335 t
ORIGIN      
        1 atacataaag atgcgttctc cctttctcat ttaaacctcc caaacctcag tactgtttac
       61 atgagattca ggatagcctt attttaggaa gtgtaaaatc ttatttagca aaatttgtaa
      121 aattcagttt agcacccaca actcctgagt acagccttga tctgtcggtc tacggacaac
      181 ttctgggagg tgcagcatgt ctaaagcgct aatttcagaa ctttgccggc agctgcgtct
      241 gggaacttac attgctgaca gctatacaga ggttgaggct gatagccacg aagagttttt
      301 gattaagctt ttgacagagg ctgtcgctaa gcgcagtact gagcgacgca agcgttatat
      361 ccagcaggca ggtttcgacc tgatgaaggg ctatgatgat tttgatttct cggatcttgt
      421 tttacctgcc ggacttacac cggagagttt gcgtacttgt gattttgtgg aaatgcagca
      481 gaatctgatt ctttacggga ggccaggaac aggcaagaca cacctggcta ttgctcttgg
      541 catagaggct tgcaagcgtg actacaaagt cctttactgc aaaacaagcc gcctggttaa
      601 ccttttgtcg caagccaagg aagagataag cgaaagcaag ttctggcgaa aactaaacac
      661 agctgatttg ttgattttgg acgagtgggg ctacatccct tttgaacggg taggtacaca
      721 gcttctcttt gaggccattt ctgattgcta tgagaagaga agtgtgatta ttacaactaa
      781 tttgcctttt gatgaatgga acacaatatt ttacgaccag aaactgactg cagccatctt
      841 ggatcgtttg atccaccatg gactgctgct gatgcacgat ggacaaagct accgcttgca
      901 gaactcacaa atgcaatagc ggagcacagg attattcgaa cgatgcgggt ggaaatactc
      961 ttaacttccg cagtggcggg tttttaactt ccgcgctggt agacttttag cttccgcgct
     1021 ggctgctttc atgcttgcca aacacagcga gccacaaaga aattagaaca agaatataaa
     1081 aaccgtcaga tttgaccctc tcccaaggct actaggtagg aggtcaagac ctccaaaaaa
     1141 acacaaggag gtaattcgaa tggaattaca agtttacaaa aatgcagagt tcggctctgt
     1201 acgtactaca acgattggcg gtcaacctta ttttgtcgg
//
LOCUS       DASRMS010000181         9255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_787838, whole genome shotgun sequence.
ACCESSION   DASRMS010000181 DASRMS010000000
VERSION     DASRMS010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 9255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9255
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_787838"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VFC74_07995"
     CDS             <1..1448
                     /locus_tag="VFC74_07995"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HZK25314.1"
                     /translation="IKRGASTLLNARLIPVIDDFLKAIKNSLQKRNITAPVVIVRSDG
                     SLMSERFTTVRPVETLLCGPAASVMGGIELTGEKDCLIVDIGGTTTDMALVKHGVPVK
                     AKDGVNVGKWQTFVKAVYVDTFGLGGDSAVRVDRNGTMSLGPSRVVPFAIAAEQWPSI
                     TEDLKRLVKSRVSLVNPTYEFICLVKDIAGKDGFTKDEQVLCAALQAGPLMLNEAMKV
                     IGKRIFTPYTERLEREGIIMRCGLTPTDFMHIRGDFNRFNTEAARLGAEYLANRHDLT
                     VEKLTEQVFDKVEGTLYFNIVRMLLEDRYPHYKKNGLDEELEQLIRDSWQQARNGDQE
                     EFCHINFRTPATLVGVGAPTHIFLPNVAKALGTKYIVPENAGVANALGAIVGNISVTS
                     EVEIRTQYAVGGGSGFVVFGYSGNSQASSMTEAIKIAGEEAKLEAIAEAKRRGASGDI
                     TVTVDTNGTEGKPGIMLSATISATAIGRVIL"
     gene            complement(1565..3184)
                     /gene="hcp"
                     /locus_tag="VFC74_08000"
                     /gene_synonym="priS"
     CDS             complement(1565..3184)
                     /gene="hcp"
                     /locus_tag="VFC74_08000"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HZK25315.1"
                     /translation="MFCNQCEQTAKGIACTAHGVCGKDAETSALQDLLLHAVTGLSLY
                     AVEARKKNTGIDSAINQFTVKAIFSTLTNVDFDPQNFANRINQAVQLREQLKNKVEEI
                     SVTHPAADFTPAADLAGLIAQGTLVNLTADETIDPDLQSLQQILLYGLKGVSAYADHA
                     LTLGQEDEQVYTFVHEAMASFTNKDLTMQEWLQLVLKCGEINFRAMELLDAANTSTYG
                     HPVPTPVPLGHRAGKAILVSGHDLKDLAELLKQTEGTGINIYTHGEMLPTHGYPELKK
                     YPHFYGHFGTAWQNQQKEFADFPGAILMTTNCLQKPQPSYADNIFTAGNVGWPGIQHI
                     EAYDFSPVINKALELPGFTSEQDADSVTVGFARKTVLGLAPTVIEAVKSGAIKHFFLV
                     GGCDGIKADRNYYAEFVEKTPQDSVILTLACGKFRFFDKQLGDIGGIPRLLDVGQCND
                     AYSAIQIALALAEAFDCGVNDLPLTLVLSWYEQKAIAILLTLLHLGIKDILIGPSLPA
                     FITPNVLNILVEQYNLRPISTPEQDLAMILG"
     gene            complement(3415..3735)
                     /locus_tag="VFC74_08005"
     CDS             complement(3415..3735)
                     /locus_tag="VFC74_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25316.1"
                     /translation="MTGKKHLLVFALLILAAMWLTGCSGSNNTPEDTPPNSPSVNEPT
                     LTGAELVEASCVRCHTLDRLTSQPRDDAAWHQLAERMLDKSPDLLTSAEYELVVAYLL
                     EQDQ"
     regulatory      4007..4091
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            4181..5596
                     /locus_tag="VFC74_08010"
     CDS             4181..5596
                     /locus_tag="VFC74_08010"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HZK25317.1"
                     /translation="MNKGLSRILTVMVAAILFGSSAFVVQFWPQLDKQFKSAAAEPVV
                     STLAAAEGRALTNIAELQAEIRQLKVMEAVQNAASAKTEQEKAATEAPAEAKKTTAKT
                     SPRTVVSTRQTKATVPVTKKPDTAAPVSKKTEATAPTKPVTEPQVKATPVPTQTTEPP
                     TPQASETEASVPKPEPAPKVEEKKTPANPYSVGSSREFRVLSQAIVKNTGDALATNVR
                     IQVPLITTSSLYYSRRGESFSLEPTEIKSVNGTRMGVFNLGELAPGQEVSIAVHTRVR
                     TANISFYADYVPTGGKKVTSYLGSASGIETTNGKIVSLANQLTSGLTADWDKARAITR
                     WVATNISYNASAANRNSGALTALNSRTGVCEDYAKLSAALARAAGIPARVVYGYTDSG
                     SKWPSSGSLALRGYRHAWVEFHLVGRGWVPADPTRSSSSKLYFGKLPHNRYIIQNYNN
                     QSLKGNYSGGKLSISWADSLE"
     gene            complement(5684..6304)
                     /locus_tag="VFC74_08015"
     CDS             complement(5684..6304)
                     /locus_tag="VFC74_08015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class E sortase"
                     /protein_id="HZK25318.1"
                     /translation="MRRLAKHIGLLLMLSGLLLVAAPLYRWGTGIYYQRLTLSTLEEQ
                     AEVDDKITPPLDTNQEQSKDELPSALGLLEIPKIDLKAAVMHGVAEEDLKKGPGFYPQ
                     SKHPEVGNVSIAAHRGVYGSWFRHLDRLEAGDTISLTLGTNCYQYQVREQFVTHSRDW
                     SVVESTEQAELTLTTCLFTTTTKRLIVKADLVKVTEVKTRPKARPN"
     gene            complement(6469..8955)
                     /locus_tag="VFC74_08020"
     CDS             complement(6469..8955)
                     /locus_tag="VFC74_08020"
                     /inference="COORDINATES: protein motif:HMM:NF037639.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaA isopeptide-forming pilin-related protein"
                     /protein_id="HZK25319.1"
                     /translation="MKDVKRKLLAIFLILTMLLTLVGSAVAEGGGGNPGEASGKGVRP
                     EYVDGNPDADNLGISGNSYKIDAAPQNGTYYDEHGTLIVKITNANGNHFDWTANIPVK
                     AIIVKGGNCANVYRYNPNKESDTGLHAPMNPSSGKVYGLSHVTFIYDTQIESGKGFLL
                     VKNVVLNSRGHIDWRNKEEFTFVATNQATQEQQTFSISSTQEQQIFTIRGNGRDIKKV
                     PAGTYTIKEINIPEGYKPFKSTKVVKVKEHLTTLVIFINRKDVVPPSEGTLTIEKRVE
                     ASDGNLIEDDTTEFTFAVLNGEGESVQTPTIIGNGSTDLTLPAGDYTVTETNIPEEFE
                     QDVSVQTATLASGGTAAVTFTNKLKAEPPTTGTLTINKTVVNSFNEEINDDTEFTFSI
                     TPIFRSSASPSSITVKGGSSETINLPAGEFSIAEENIPEAYENDVEAQFVIIENGYSK
                     SLTFTNKLKAEPITTGTLTITKVVRDSEGAVQEDDNTAFQFAISPAAVRPMSSTPVQQ
                     EVTVNAGSSNTVELPAGDYLVKEVNIPDGYEHEVEAQEVSIIAGEEHCLTFNNTLLDE
                     IEETNGALTLTKRVVDRDGSVLDEDDGLFAFVLTNNDTKEVQNVKIEGNGKEIITLLA
                     GSYTIKEIRIPEDYVQDIAAQTVEIKADENSTVTFTNEKLEEIIIDPPPAGGDGEEGT
                     LTINKRVIDGDDELLTADEGLFTFVITDNNTKKEQTVTINGNGKKAITLLAGSYTIKE
                     IRIPEDYVQDIAAQIVEIKADENSTITFTNEKLEEIIVEPEDPSGNDEDKDEDEKEKE
                     LPKTNAADLWMLGFGFLVTTSGWTLRRKRK"
BASE COUNT         2431 a         2014 c         2121 g         2689 t
ORIGIN      
        1 atattaaaag aggtgctagt actctcttaa atgcacgtct tatccccgtg attgatgact
       61 ttcttaaagc gattaagaat tcgctgcaaa agagaaatat tactgcgccg gtagtaattg
      121 tccgcagtga tggtagtttg atgtcagaaa ggtttaccac tgtgcgtcct gtggaaaccc
      181 ttctatgtgg tccggctgcc agtgtgatgg gtggcatcga attaacagga gaaaaagact
      241 gtctgattgt agatattggt ggtaccacca ccgatatggc acttgttaag catggtgtac
      301 ctgtgaaagc aaaggatggc gttaatgtcg gcaagtggca gacctttgtc aaagctgttt
      361 atgttgatac ttttggtctg ggcggtgata gtgctgtacg cgtagaccga aacggtacta
      421 tgagcttggg tccctcccgt gttgtcccct ttgccatcgc ggccgaacag tggccaagca
      481 ttactgagga cttaaagcgt ttggttaagt caagagtaag tttagtgaat cctacttacg
      541 agtttatttg cttggtgaaa gacatcgccg gtaaagacgg ttttactaaa gatgagcagg
      601 ttttatgcgc agctctgcaa gctggaccgc tgatgcttaa tgaagccatg aaggttattg
      661 gtaagcgtat ttttactcct tatactgaac gtttagaaag agaaggaatt atcatgcgct
      721 gtggtctaac gcctaccgat tttatgcata tacgcggtga ttttaaccgc tttaacacgg
      781 aagcagctcg cttaggtgca gagtacttgg caaataggca tgatctgaca gtggaaaaac
      841 tgacagagca agtttttgat aaggttgaag ggactttgta tttcaatatc gtccgcatgc
      901 tgctggagga tcgctatcca cattataaaa agaatggttt agatgaggaa ttagagcagt
      961 taatcagaga cagctggcag caagcgagaa acggtgacca agaggagttc tgccacatta
     1021 atttccgtac accagctact ttagttggtg tgggtgcccc tacacatatc tttttaccca
     1081 acgtggccaa agctttgggt acgaagtata ttgtgccgga aaatgctggc gttgccaacg
     1141 ccctaggcgc cattgtggga aatatttccg tgacaagtga agtggaaatt agaactcaat
     1201 atgcagttgg tggcggcagc ggctttgtcg tgtttggcta ctcaggcaat agtcaggcgt
     1261 caagtatgac tgaggcgatc aaaatagcag gtgaggaagc taaactagag gccattgcag
     1321 aagccaaaag acgtggtgcc tcaggcgata ttacagtgac agttgataca aatggcactg
     1381 agggaaagcc aggcatcatg ctctctgcca ccatctcagc cacagccatt ggccgggtta
     1441 ttctttagga aacactaata agcacttgca gcgtaacgta actactgaat tatataattg
     1501 gttcagtaaa aaaaaaccgg aaagcttaag gctttccggt ttttgtcatg ccagtgagtt
     1561 ttgcttagcc caagatcatg gccaggtctt gctctggcgt ggagatgggg cgaaggttgt
     1621 attgttctac gagaatgttt aagacattgg gggtaataaa agcagggaga ctaggtccaa
     1681 tgagaatgtc tttaatgcct agatggagca gtgtcagcag gatggcaatc gctttttgtt
     1741 cataccagga aaggacaagg gtcagcggta agtcattaac gccacagtcg aaggcttctg
     1801 ctaaagctaa agcaatttga atggcagaat aggcatcatt acattgaccg acatctaaga
     1861 ggcgggggat accgccaata tcgcccagct gtttgtcaaa gaagcggaac ttgccacagg
     1921 ctaaagttaa aattacactg tcttgcggcg ttttttccac gaattcggca taatagttac
     1981 ggtcagcttt gatcccgtca cagccgccta cgaggaaaaa gtgtttgatg gcaccgcttt
     2041 ttacagcttc aatcactgtc ggtgccagcc ctagtactgt tttacgcgca aagcccacgg
     2101 ttacactgtc tgcatcctgc tcgctggtaa agccgggtaa ctctaaagct ttgttaatta
     2161 cagggctaaa atcataagct tcaatatgtt gaatgccagg ccagcccaca ttaccggcag
     2221 taaaaatatt atcagcataa ctcggctgag gtttttgtaa gcaatttgtg gtcattaaaa
     2281 tagcaccagg gaagtcagca aattcttttt gttggttttg ccaagccgta ccaaaatgtc
     2341 cgtagaaatg cgggtatttt tttaattctg gataaccatg agtaggcagc atttctccgt
     2401 gtgtatagat attgatccct gtaccttctg tttgttttaa tagttcagct aaatctttta
     2461 aatcatggcc agagactagg atagctttac ctgccctatg acctaaagga actggtgtcg
     2521 gcactgggtg gccataggtg ctggtattgg cggcatcaag tagttccatg gcacgaaagt
     2581 ttatttcgcc acattttaaa actagctgca gccattcctg catagttaaa tctttattcg
     2641 tgaaggaggc catcgcttca tgcacaaacg tatatacttg ttcatcttcc tgccccagcg
     2701 ttagggcgtg atcagcatag gcagagacgc cttttaagcc atacagcagg atttgctgca
     2761 gtgattgtag atcgggatca atcgtttcat cggcagtaag attaactagt gtgccttgtg
     2821 ctatcagacc cgcaagatct gcagcagggg taaagtcggc tgccggatga gtaacgctta
     2881 tttcctcaac tttgtttttt agttgttcac ggagttgaac agcctgatta atccggttag
     2941 caaagttttg tgggtcaaaa tctacattcg tcagggtgga aaagatggct ttaacggtaa
     3001 attgattaat tgcactgtca ataccagtat ttttctttct tgcctcaacg gcatataggg
     3061 aaagtcccgt caccgcgtgg agtaataagt cttgtagagc tgaagtttca gcatctttac
     3121 cgcagacccc atgtgctgta caggctatac cctttgcagt ttgttcgcat tgattacaga
     3181 acatcttcaa ttaaatctgc ctccttgatc ttgtttagtg gttggaaatt atatcgctgg
     3241 cctgaagcaa gcggcttttg tcgccagtac aaaagctttc tgtcaaattt cttgtgtcct
     3301 tattgtaatc agatgccagg cggcaggact gtgatggata tcacggtata aaactttctt
     3361 tccccgcaaa agaacaccag gctccagtgc ctggtgtcaa taatctttat gctgttattg
     3421 atcttgctcc aataaatatg ccacaacaag ctcatattct gctgacgtca gtaaatctgg
     3481 tgacttatct aacatccgct ctgctagctg gtgccatgca gcgtcatctc taggttgact
     3541 tgtaagacga tcaagcgtat ggcatcttac acaacttgcc tccactagtt cagcgcctgt
     3601 cagtgttggt tcattcacag aaggtgaatt agggggggtg tcctccggtg tattgttgct
     3661 gccgctgcaa cctgtcagcc acatagctgc cagtattaag agggcgaaaa caagtaaatg
     3721 ttttttacca gtcattagta tattcacctc cgagtcattt ctacaataca ttacgttctt
     3781 cggtctggac aaatttcctt cttttccggc agaaaaatac aacataacaa agtggaaata
     3841 ataaataaag ttttggtgat aaagcaggaa tttaccaatt aatgcagaat aacgtagaag
     3901 ggatctacac gaaaggtgcg gttagtcagc tattttacgt gcacctatag tacagaacat
     3961 acaccggtgt ggggccggta attaaaaatt cggtaggggg tctcctatcc gacaaaggca
     4021 aacttgctga aaagcaagga cgcaaagcca tgggtctacg ggctgctgcc tatgatcgcc
     4081 aggttgccga agagcggagt tttttgctat ctagcttcag ctccggctga agcttttttg
     4141 attcggctgt cgcagggctc ccagaaaatg ggggagaaaa atgaataaag gtttatcaag
     4201 aattctaaca gtgatggtgg cggctatctt gtttggcagt agtgcatttg tcgtacaatt
     4261 ttggccgcaa ctggacaagc agtttaagtc tgcagcagcg gagcctgtcg tgtcgacatt
     4321 ggctgccgca gaaggtaggg ccttaacaaa tattgctgaa ctgcaggcag aaattagaca
     4381 gttaaaagtg atggaagctg tgcaaaacgc agcgtccgca aaaacagagc aagaaaaagc
     4441 agcaacggaa gcaccagcag aggctaaaaa aacaactgct aaaacatctc ctagaactgt
     4501 tgtctctacg agacagacaa aagcaacggt tcctgttaca aaaaaaccag atactgcagc
     4561 accggtctca aaaaaaacag aagctaccgc accgacgaag ccggttacag aacctcaagt
     4621 caaggcaaca ccggtaccaa cacaaaccac ggagcctccc actccgcagg cttctgaaac
     4681 agaagcctct gtcccgaagc ctgaacctgc accaaaggtt gaggaaaaga aaacgccggc
     4741 taatccttat agtgtcggct ccagcagaga gttccgtgtg ctctcacagg ccatagtaaa
     4801 aaataccggt gatgcactag caactaatgt gcgcattcaa gtcccgttga ttactactag
     4861 ttcactgtat tatagtcgcc gcggggaaag cttttccctg gagccgacag agataaaatc
     4921 ggtcaatggt acgaggatgg gtgtctttaa cctgggagag ttggcaccag ggcaggaagt
     4981 gtctattgct gtgcatactc gcgtcagaac agcaaatatc tccttttatg ccgactatgt
     5041 gccaactggc ggcaagaagg ttacttccta tttaggtagt gccagtggta ttgaaaccac
     5101 aaacgggaaa atagtcagtt tagccaatca gctcacctct ggtctgacag cagattggga
     5161 caaggctcgg gccatcacgc gttgggttgc cactaacatc agctataatg ctagtgcagc
     5221 taaccgcaac agtggggcct taacggcact taatagccgt actggtgtct gtgaggatta
     5281 cgcaaagtta agtgctgctt tagcccgtgc tgccggtatt cccgctagag tggtttatgg
     5341 ctatacagat agtggcagta aatggcccag cagtggctcc ttagccttac gtggttacag
     5401 acatgcttgg gtagagtttc atctcgtggg tcgtggttgg gttcccgcag accctactcg
     5461 cagcagttcc tctaaattat attttggtaa actaccgcat aatcgctaca tcattcaaaa
     5521 ttataataat caatccttaa aaggaaacta tagcggcggt aaactatcta tcagttgggc
     5581 agattcactt gaataaggaa cccctatcac cccccaagtg atagatccca aagagccgga
     5641 cagttaaatg tccggctcca cttattaata cgggtaagca gctttagttt ggtcttgctt
     5701 tagggcgtgt cttcacctca gttactttta ccaaatcggc cttaacgatc aggcgttttg
     5761 tagttgttgt aaataagcag gtggttaggg ttaactctgc ttgctctgtg gattcaacta
     5821 cactccagtc acggctgtgg gtgacaaact gttcgcggac ttgatactga tagcagttgg
     5881 tgccgagggt taaagaaatc gtatcaccag cttcaagacg gtcaagatgg cgaaaccagg
     5941 aaccgtacac acctcggtgt gcagcaatag aaacattgcc gacttccgga tgtttgcttt
     6001 gcgggtaaaa cccgggaccc ttctttaagt cttcttctgc aacaccgtgc atgacagctg
     6061 ccttgagatc aatcttggga atttccagca aacccagggc agaaggcagc tcatctttgc
     6121 tctgttcctg attggtgtcc aaaggaggcg ttattttatc gtctacttct gcttgttcct
     6181 ccaatgtact taatgttaag cgctgataat aaataccggt tccccagcga tagagggggg
     6241 ccgcgacaag cagtaagcca ctgagcatta gcagtaaacc gatatgtttg gccaggcgac
     6301 gcatcgaaaa cacctcactt ttgcactttt aaagccagac atattatacc ataagtagct
     6361 gcaggtatat gaagattaat ttatcatttt gatattatct gccattttta cgttagtcct
     6421 actgacgaaa aagcaggaac ccttgagcag ggttcctgcg aatttgtttt atttgcgctt
     6481 ccggcgcagt gtccagccgc ttgttgtgac taggaagccg aagccaagca tccatagatc
     6541 agcagcatta gttttcggca attctttttc tttttcatct tcatccttat cctcatcatt
     6601 accactggga tcttctggtt caacaattat ttcctctaat ttttcgttag tgaacgtaat
     6661 agttgaattt tcatcggctt taatttctac gatctgggca gcaatatctt ggacatagtc
     6721 ctcaggaatc ctaatttcct taattgtgta gctgccagca agcagggtaa tagccttctt
     6781 gccattacca ttaatcgtca cggtctgttc ttttttggta ttgttatcgg taataacaaa
     6841 ggtgaacagc ccctcatctg cagtcagcag ttcatcgtct ccatcgatta cccgcttgtt
     6901 aatggtcagc gtaccttcct caccatcgcc accggctggt ggtgggtcaa tgatgatttc
     6961 ctctaatttt tcgttagtga aagtaacagt tgagttttca tcggctttaa tttctacggt
     7021 ctgggcagca atatcttgga catagtcctc aggtatccta atttccttaa ttgtgtagct
     7081 gccagcaagc agggtaatga tctccttgcc attgccttca atctttacgt tttgtacttc
     7141 tttagtatca ttgttggtca ggacaaaggc gaacagtcca tcatcttcgt ccagcacact
     7201 accgtccctg tcaactaccc gtttggttag tgttaatgcg ccgtttgttt cttctatttc
     7261 atcaagcagg gtattgttaa atgtaagaca atgctcttca ccggctataa tgctaacttc
     7321 ttgagcctct acttcatgtt cgtatccatc agggatattt acttctttga ccaagtaatc
     7381 tcctgcaggg agctctactg tgttcgaact gccagcgttt acagttacct cttgctgaac
     7441 aggggtgcta gacattggtc tgactgctgc aggtgatatc gcaaactgaa atgctgtgtt
     7501 gtcatcctcc tgtacagcac cttcgctatc tctaactact ttggtaatgg tcagggtgcc
     7561 cgtggttatt ggttctgctt ttaacttgtt ggtaaaagta agactcttgc tgtaaccatt
     7621 ttctatgatc acaaattgtg cttccacatc attttcgtat gcttcaggga tgttttcttc
     7681 tgcaatgctg aattctccgg caggaagatt aatggtttcc gagctaccgc ccttgacagt
     7741 aatactcgac ggcgaggcag aggagcggaa aatgggcgta atgctaaagg taaattctgt
     7801 gtcgtcatta atctcttcgt tgaaactgtt tactacagtt ttattaatcg tcagagtgcc
     7861 cgtggttggt ggttctgctt ttaacttatt ggtaaaggta actgcagcag tacccccgga
     7921 ggcaagtgta gctgtttgta cagatacatc ttgctcaaat tcctcgggaa tgtttgtttc
     7981 cgtaactgtg taatctccag caggaagtgt aaggtctgtg gagccattgc ctatgattgt
     8041 tggtgtttgt accgattcac cttcaccatt aagtaccgcg aaggtaaatt cagttgtatc
     8101 atcctctatt aagttgccgt cactagcttc gactcttttt tcaattgtca gcgtaccctc
     8161 acttggtggt accacatctt tgcgattaat aaatataact agtgtggtta aatgttcttt
     8221 aacctttact acttttgttg atttaaatgg tttatagcct tcagggatgt ttatttcctt
     8281 aatcgtataa gtaccggcgg gtactttttt gatgtcacgc ccattgcccc tgatggtgaa
     8341 aatttgttgt tcctgggtag agctgatgct gaaagtttgt tgttcctggg tagcctgatt
     8401 ggtagcaaca aaagtgaatt cttctttatt tctccagtcg atatgccctc tactatttag
     8461 cacaacattt ttgacaagca gaaaaccttt tccgctttca atttgtgtat cgtagatgaa
     8521 ggtcacatga ctgagaccat aaactttgcc tgaacttgga ttcattggtg catgcaagcc
     8581 agtatcgctc tctttgtttg ggttataccg atagacgttt gcgcaatttc cacctttaac
     8641 aataatcgct tttacgggaa tattagctgt ccaatcaaag tggttaccgt tggcgtttgt
     8701 aatttttaca attaaagtgc catgctcatc ataataggta ccattttgcg gtgcagcatc
     8761 aatcttataa ctattaccgc ttatccccag attatcagca tccgggttcc catctacata
     8821 ctcagggcga acacctttac cgctggcttc ccctggattt ccacctccac cttcagcaac
     8881 tgcggaaccg actagagtta agagcatcgt taagattagg aagattgcta ggagttttct
     8941 ttttacatct ttcatcttat taaacctcct ttttttaccc tggccccctg ctccccccaa
     9001 gcaacatgcc agagtcagat tgaaccgatt tctttctttc acctgtcaaa aattaagaat
     9061 taatgagtta tcgtacagaa attgttgtaa tttgtgcgga taagtattgt aaatcgtcaa
     9121 tttttggcaa taaaaaagca cacgggtgta aaacccgtgt gcagccggta tcactctatc
     9181 tcccacgcct cataagtgac ctgatcacga agcggtgttc attgaatccg cacactacag
     9241 caacagttat tgcta
//
LOCUS       DASRMS010000182         1138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_187725, whole genome shotgun sequence.
ACCESSION   DASRMS010000182 DASRMS010000000
VERSION     DASRMS010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_187725"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..>1138
                     /locus_tag="VFC74_08025"
     CDS             29..>1138
                     /locus_tag="VFC74_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZK25320.1"
                     /translation="MQSQRARTTLLASSGENRAALAQRYSQAGMIFSADGEKLAYSDT
                     DGHRSYSSDPLLAMAALHVVGDYTHNIAYTVESLWQNELLGEGRSFAEQLKLDLLGQG
                     LQGNDLILTLRSDLMKKAYSLLDGRRGAAVVLNYETGAILASVSSPSVHPDKVVTWQD
                     IPDTALFNRAFSGEYVPGSTFKIITAAALLQSPFSTEDTVICQGTTPLMPGGVSETHI
                     NAGHGEVSLQAAMTQSCNHYFGFMGLKMGPKKIISAAEQMAFNQKIELDRFHLAKSKC
                     LIGREDDPALTWAAIGQAAGEDEITVSPLHLALISGMIANNGRMVEPYLVESMVYPSA
                     VETNVSKRGSLMAQIDPGLAQELTELLRQVVSSGTG"
BASE COUNT          300 a          239 c          303 g          296 t
ORIGIN      
        1 gtttattctg tttacggtag gagtcgtttt gcaaagccag cgtgcgcgaa caactttatt
       61 ggcatcctca ggagaaaaca gagcggctct ggctcagcgc tattcacagg cgggcatgat
      121 ttttagcgca gatggcgaaa agctggctta ctcagataca gacggacaca ggagctatag
      181 cagcgaccca ttgctggcta tggctgcgct acatgtggtc ggggattata cacataatat
      241 agcatatacg gtcgagtcct tatggcagaa tgaacttttg ggtgagggga gatccttcgc
      301 agagcagctt aagttggatt tgctgggcca gggcctgcag ggcaacgatc tgatactaac
      361 tttgcgtagc gatctgatga aaaaagctta ttcgttgctg gatggcagac ggggtgctgc
      421 agtagtatta aactatgaga ccggagctat tttagcctcg gtttcttcgc cctctgtgca
      481 tcctgataaa gttgtaacct ggcaggatat accagacacg gctcttttta accgtgcttt
      541 cagtggcgag tatgtccctg gatctacttt taaaataatt acagcagcag ctttactgca
      601 aagtcctttt tctacagaag acacagtcat ttgccagggt actacgccgc ttatgcccgg
      661 aggggtgagc gaaactcata ttaacgcagg tcacggtgaa gtcagcctac aagctgccat
      721 gacgcagtca tgtaatcatt attttggttt tatggggtta aaaatgggac ccaaaaaaat
      781 aatcagtgca gctgaacaaa tggcatttaa ccaaaagatt gaattggatc gctttcatct
      841 ggcgaaaagt aaatgtttga ttggtagaga agatgatccg gctttgacct gggctgctat
      901 tggccaggcc gcaggtgaag acgaaatcac agtcagcccc ctgcatctgg ccttgatatc
      961 aggaatgata gctaataacg gacgtatggt agaaccttac ttggtagagt ctatggttta
     1021 cccttcagct gtcgaaacca acgtcagcaa gcgtgggagt ttgatggcac agattgatcc
     1081 gggcttggct caggaactca cagaattgct gcgccaggta gtaagcagtg gtacaggt
//
LOCUS       DASRMS010000183       116274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_49187, whole genome shotgun sequence.
ACCESSION   DASRMS010000183 DASRMS010000000
VERSION     DASRMS010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 116274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 116274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..116274
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49187"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..260)
                     /locus_tag="VFC74_08030"
     CDS             complement(<1..260)
                     /locus_tag="VFC74_08030"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HZK25321.1"
                     /translation="MREELLKSFVDMDEREAVRIAKELVETDGGPVAVLETCKEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMAFTLPKLQQGDVSSIGT"
     gene            complement(365..1597)
                     /locus_tag="VFC74_08035"
     CDS             complement(365..1597)
                     /locus_tag="VFC74_08035"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25322.1"
                     /translation="MTFDAKKLAAERAARYKAAVELKEPDRVPFTGLGGDVVAAYAGI
                     TGYQYCFDYELANQAALKYLKDFPSDLPMLGMPSLGNSVTSLAFFDYPDLAPLVSSVD
                     GPTNDILGVKYCRFPGRELDENSSPQFIGSAYMEPEEYDELIADPAKFMFEKVVPRTT
                     TNLQNLGSPTALAAMARLGLEKSKQGAAMQNMIKELQELGYAPSGRAVFIAPLDFIGD
                     QLRDIPNIIKDLRRYPDKVKAAAEALLEPLLKMVLSGKARGIDISFIPLHLNEYLSPK
                     LYHEFYWPTLKEGILGMLQEGMKSQVFFEGHHDAHLETILDLPAGWGIGYFEKTDVRK
                     AKKLLQGHTCIMGGIDTGMIITSTPAKLDEYIKELLGEMMPGGGFILSPNIGTLPRET
                     PIENIRAVYEAVEKYGKY"
     gene            complement(1787..3013)
                     /locus_tag="VFC74_08040"
     CDS             complement(1787..3013)
                     /locus_tag="VFC74_08040"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25323.1"
                     /translation="MDAKKLAAERAARYQAAVELKEPDRVPFTGMGGDVVAAYAGITS
                     YEFCFEPEKAQQAVLKYLKEFPSDRPIMGGGGIGHAVAQLAFLDFPDLAGQIGMIDGP
                     TNDILGVNFIRFPGRQTDENSSPQFIGQAYMEPAEYDELIANPTKFLYEKIVPRTTTN
                     LRDLGSPTALAAMARFGMQKAKAAAASQGFVQELQALGYPLSAGSFFLAPLDFISDNL
                     RDTVNLVKDLRRYPDQVKAAAEALLEPMLKLALFGKNIGAEIAFIPLHLNEYLSPTFY
                     QKFYWPTLKAGILGMLKEGMKSQVFFEGRHDAHMESILELPAGWGIGYFEKTDVRKAK
                     KLLKDHTCIMGGIDVGMVIGSTPAKLDEYIKELLGEMMPGGGFILSPNIGTLPRETPM
                     ENIRAVYEAVEKYGKY"
     gene            complement(3178..4410)
                     /locus_tag="VFC74_08045"
     CDS             complement(3178..4410)
                     /locus_tag="VFC74_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25324.1"
                     /translation="MTIDAKKLAAERAARMKDAIALKEPDRVPFTGMGGDVVASYAGL
                     TSYEFNFDYEKQSQAVLKYVKDFPSDRPAMGMTGLGNHVASLAFLEYPDIFPIISRVD
                     GPTNDILGVNYCRFPGRELGENSSPQFIGSTYMEAEEYDALIADPVKFCYETVLPRMV
                     TAFKDLKSPTAHAAMTRLALEKAKASAAMRDLFVQLQELGYGPGGQSIFFAPLDFIGD
                     QLRDIPNLVKDLRRYPDKAKAAAEAVLEPLLKVSANAQGPGVDTAMIPLHLNEYLSPT
                     LYNEFYWPTLREGILRLAELGMKSQVFFEGRHDPHMETILDLPVGWGTAYMEKTDVRK
                     AKKMLQGHTCIMGGIDVGMVIAGTPAKLDEYIKEILGEMMPGGGFILSPNIGNLPRET
                     PIENIRAVYEAVERYGKY"
     gene            complement(4423..5046)
                     /locus_tag="VFC74_08050"
     CDS             complement(4423..5046)
                     /locus_tag="VFC74_08050"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25325.1"
                     /translation="MRKELLKNFVDMNEVEAVSIAKALVETDGGPVAVLETCKEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMAFTLPKLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGIHVIDIGIDVPHEKFVEAIKEHQPDIVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLA"
     gene            complement(5137..6372)
                     /locus_tag="VFC74_08055"
     CDS             complement(5137..6372)
                     /locus_tag="VFC74_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25326.1"
                     /translation="MTVKDPQSLAEEREARFFGAMELKATDRVPFTGLGGDIVAAYGG
                     ISNAEYTFDYDLQRQATVKYLRDFPSDLPASGGAGIGNIFLSLAFLDFPDLSTSIAFT
                     TGPYNDVLGVKFCRFPGRELPDNSSMQYLGGAYMEPKEYDELIADPVKFSYEKIVPRT
                     STNLENLSSPTALATMARLGIEMAKHRASSRALGKELAALGYPSVVRGLGWAPLDYIG
                     DQLRDIPNLVLDLRRHPDKVKAAVEALMEPLLKMSLMYKDVGAKYIFIPLHLNEYLSP
                     KLYNEFFWPTLKEIILKLFEAGLKSQVFFEGWQDPHLETILELPAGWGVAYFEKTDVR
                     KAKKLLQGHTCIMGGIDVSDIISSTPAKLDQYVKELFAEMMPGGGFIFSPNIGYLPRE
                     TPIENVRAVYEAVEKYGKY"
     gene            complement(6520..7758)
                     /locus_tag="VFC74_08060"
     CDS             complement(6520..7758)
                     /locus_tag="VFC74_08060"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25327.1"
                     /translation="MVSPEMKKLAQERAARMQAATDLKEPDRVPFTGLGGDVIPAYAG
                     LSSYEYEFDFEKNRQAAIKWFSEFTSDSAPRVGVGGLDNQSMSVAFQDYPDIAGGVGA
                     INGPVNDILKMKFVRFPGRELDEYSARQFIGGSNMDPSEYDQLIADPQKFCFEVIMPR
                     ISGSLKDLSSPASLATMARFGIEKARQDAEMAKTVQAMMELGYSFSPRGVCLAPLDLI
                     SDQLRDIPNAVLDLRRYPDKVKAAAEALVEPMFKMGTMALKAGAKECFIPLHLNEYLS
                     PKQYNEFYWPTLKEVILKLVAAGMKAQVFFEGWHDAHLETILELPAGWGTAYFEKTDV
                     VKAKKLLKGHTCIMGGIDVSDIIALTPAQLDEQIKKLFDEMAPGGGFIFSPNIGTCPR
                     DTPIENVRAIYEAVEKYGKY"
     gene            complement(7771..8394)
                     /locus_tag="VFC74_08065"
     CDS             complement(7771..8394)
                     /locus_tag="VFC74_08065"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25328.1"
                     /translation="MQTQLLKSFVDIDEVEAVRIARELVEGDGGAVKVLETCKEAMEE
                     IGRLFEAGEYFLSELIAAGDIFNQIMEFTLPRLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGINVIDIGIDVPHEKFVEAIKEHQPDIVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLG"
     gene            complement(8765..10738)
                     /locus_tag="VFC74_08070"
     CDS             complement(8765..10738)
                     /locus_tag="VFC74_08070"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZK25329.1"
                     /translation="MKKKLFFLMYILMILLIAVGCAGSSFPEPDEIIELFIQHWNSEE
                     YESMYQLLTDEAKTIHDENTFISRYNNISSGIGLKAITIHEVEVGEIVDEQVTVSYTL
                     EFETSTVERFSHHYQATLHKGEESWFLAWDHNLVFPDLEAAYAARVTRHFPQRGSILD
                     RNNKPLAYSGTVREIGVVPGKIGDENFLLNSLASLLELSPEAIEKAYTQSWVMPEMYV
                     PIKKISEAYWQEKSEQFLALQGVMIKQVQGRVYDVPPTLGQTVGYLSEITKEKLEDLR
                     PLGYRAGDYIGFTGLEAAYETTLAGTIGFAITIYDAANNAAATVAETAVVDGEDLITT
                     LDSDLQFVTDKVLAEQTGSVIILDSISGEVLTMGSNPGYDSNLFALGITQAQYSRLQE
                     KNSPFANRALTGQMPPGSTFKPFTALMALAENIFDPAESWDTPQKWQASSGWGNHYVT
                     RVGRPAGAVNLRRAMVYSDNVYFAALSVKLGADKFTRHAKLLGFEETIPFPLTVHSSK
                     INTKPLSDVLLADSGYGQGELQMSPLHLALMFSLFPRGDGTIPKPQFFLGELPQTWLD
                     TGYAPENIRLVDETLRAAIQEPGAVAGAGNLEGLDIKGKTGTAEISGQRQLGWYACYF
                     DHYVIVVALDGDTTMNSRQAIDIANQIIRLGEF"
     gene            complement(11014..12420)
                     /locus_tag="VFC74_08075"
     CDS             complement(11014..12420)
                     /locus_tag="VFC74_08075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZK25330.1"
                     /translation="MSAPQRVTNVVVNTYGLANLGFSLMVSMTSNYYAFFLTDVLLMP
                     AAVMATLMMVLNLFDMLTVPTAGVIMQKSNLPWGKNRSWMLIGPPITLITFTLIFTNP
                     PLEGVFRYLWIIIIYTIAHGSYNLSFGALSAILPILGKTPEDRAILSIRRLQFSSISR
                     FIFGAISMPMLLFFTARGATVPAVRGYTFTSLVLSSVMLTMFWLVFKVTEPYETRNNS
                     QSAAQQKKSNISGKEMLQQLFKSPPLLALIFGETARNVAAFALTYTIAYYFRYIIQNM
                     SLMPVFMSSIAVIGVVASLLVQLVSKYMDKRHIYTAGLVLCLVGHTLAYLFARDLITF
                     IIFMGINQVGVSFMMGISFAMFSDCCDYGELKTGKVATPFIMNLTNLSPKLGKIVSSG
                     VIGFSLAAIGYVAEMEYTPQLAANIRAIIHLVPMLFIAVALVITTLFNKLTIETVLNM
                     QNQLRANREADSASQAGV"
     gene            complement(12710..13000)
                     /locus_tag="VFC74_08080"
     CDS             complement(12710..13000)
                     /locus_tag="VFC74_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25331.1"
                     /translation="MIQPFQVFNDIEKDKRDYGIVCIGGWDAFGPGNQTDSDEEAVRA
                     SVRLALDSYAPTGRYVFWCSGATERNPERVKWLNEEADNYGRTFYQKYERFP"
     gene            complement(13117..14031)
                     /locus_tag="VFC74_08085"
     CDS             complement(13117..14031)
                     /locus_tag="VFC74_08085"
                     /inference="COORDINATES: protein motif:HMM:NF015056.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HZK25332.1"
                     /translation="MKLLNHDQVFYAFAPTLKPLLMVAQHEEFILNTCDCFANQLSSD
                     ADTLDKLDWDRINPATGPVAIEGVKPGDLVRIDIKKLEMTGKSVMTTIPGAGMVSGIT
                     EASTRVMDNDNGVLSVSTEKGDLHLPLKPMIGVIGLAPQEGSIPNGTPGKHGGNMDCN
                     LIGEGASLYLHAAVDDGLFGCGDAHALMGDGEVLVCGAETPAQITLAAAVVDEPQLPT
                     PFVETAELYATIATAKTSDKALKEAVDNMFDFLTGIVGLSQGDAGRLMSLVGNLKFCQ
                     VVDPELTIRFEFPKAVLAQLGFAGIGAS"
     gene            complement(14028..15176)
                     /locus_tag="VFC74_08090"
     CDS             complement(14028..15176)
                     /locus_tag="VFC74_08090"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZK25333.1"
                     /translation="MNSAKSFSPKQESNKRSAVRDFIEYFDFTRKSSTVVFLIFILTA
                     IGFFIWWLNIPFIEDIFTAATITLIMTLGFQLYMGNSGILNWSYVGYVGIGAFTSAIF
                     SMTPAVKAMQIPTMYPFLINLHLPFPLALAAGAVVAMIVAAIVAWPMMRLSDAVGAIT
                     QFALLIVINVMLSQWSQVTNGPRTFTLGGTQRLTTLWVALGVAVLVIIAVYYFKESSL
                     GLKLRATRDDRHAARSSGINFVAVRYFGYVISACIGGLAGGLYSHYILSITASSFYMS
                     ELFLILSMLVIGGSMSVSGAFFGTLLVTLLRQGLRQAELFLTRSGFEASGSTEIVVAI
                     MMIIFLVWQADGITGGRELTWQSIKRRLPSGSKKRDKMIKNKKQEEEA"
     gene            complement(15173..16075)
                     /locus_tag="VFC74_08095"
     CDS             complement(15173..16075)
                     /locus_tag="VFC74_08095"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HZK25334.1"
                     /translation="MNAEILLEFAINVLSMGSLYALISLGLVFVFGICQLVNYAYGEF
                     ITVSAYVLYFLGAFTSLPWPVVFLLGIASGVAVGFLSELVAFRAFRERSLDALLVTSF
                     AVSVILQRLFQLGISPRSKAVPIPAFFNMNIQILGVITPLRNLLIIGTTVVLLTALVL
                     IMKYTTLGVAMRGASENFVTARLMGVPANLMISVAFIISGFLAGVVAIFWSARTGSVE
                     PMMGATPLLIGFIAVVIGGMHSLPGSVVGGFVYALIANVLSLTLPSSLLSFRDAFMFA
                     IVIAFLVLKPEGLLRGNYQEERVG"
     gene            complement(16075..16812)
                     /locus_tag="VFC74_08100"
     CDS             complement(16075..16812)
                     /locus_tag="VFC74_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25335.1"
                     /translation="MTNNPVLEISDLYVAYGSIQALHGICMEVHQGELVSICGVNGAG
                     KSTVLKAIAGVLKPRSGSIRFKGKDVTGSKPEHLHKLGMALVPEGREIFPSLTVEENL
                     RIGAFGRRLDKSYAADLEDLFTLFPILKERYHQAGGLLSGGEQQMLAIARGLISNPDL
                     LMMDEPSLGLSPALVDQIFELIIELKSRGITILLVEQNAQRALGIADRAYLLSNGKIE
                     YSGRPQDLEGSVDITSVYLGGQNGGDV"
     gene            complement(16809..17540)
                     /locus_tag="VFC74_08105"
     CDS             complement(16809..17540)
                     /locus_tag="VFC74_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014829331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25336.1"
                     /translation="MESKSTFFVRALSKSFAGLKAVDDVSLHLETGEILGLIGPNGSG
                     KTTLINLMTGMLAKDSGQILLDNHNIGDEPAYRVPFYGLVRTYQTIRIFQNLSCLENA
                     MIGAIGVGTKRREAAHLAVALLGELGLGDKIDLPASGLTFRDQRLLEIARALAAQPKF
                     LLLDEPAAGLNEEETDGLLSLLRSMPDNKKIGIIIVDHDMSLIMNLCNRLHVLNYGKT
                     IAEGIPEDVRKNPEVVKAYLGSAAS"
     gene            complement(17672..18874)
                     /locus_tag="VFC74_08110"
     CDS             complement(17672..18874)
                     /locus_tag="VFC74_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK25337.1"
                     /translation="MKRRIIFLLLVVLMAVGLMITATGCRREETGPKTDGDTIIIGGA
                     MSMTGIQGPLDAPAAEGIEVAIKEINANGGIDGKMLEFRNMDSKSDSATAAEVAKQLI
                     DQGAAAIITSSDYDFGGPAARAAQEAGIVGISPSASSPLFGSTMLGDKQFTLSMWNTT
                     MGAVMAEKAYVKDGLKTVYVVTDDFIDYTKSLSRYFVECYEKLGGTVLREDVFSQGKA
                     DTAALIAHYKALPETPDFIYISSYMPDLGTIIKEFRTAGIDSPIYGGDAYDDPALFDL
                     LGPDFGNDIFWDSHSYNSEAAVPGFNEYNAAYKELHGRDVDAPWSMSGYDVVMVLAEA
                     MREGGLEGDKMAKYMEDTTFELLTGKLDWSDAASGHEPNKAAAVMELVKGKSVFLEWV
                     TPDYLPRP"
     gene            19178..19783
                     /locus_tag="VFC74_08115"
     CDS             19178..19783
                     /locus_tag="VFC74_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25338.1"
                     /translation="MTKNTPITKDWLMLGMLTGVGGAIAKELVNYGLFKKNIPTVRYA
                     SIAGGAVLGKRATFGIIPKKPRTTAEWAIGHVGDIVLGGLFGSTLSYVISKSPPGNEI
                     TKGIGGGVTLWATTLAFGNLLNLDGLARIKPNQMASLLAVSALFGGTQGYFLGKYREG
                     LIEPSYPIKVQETSDDQYKSRRMGNRRTRNMEKHTNQPIMH"
     gene            complement(19948..20024)
                     /locus_tag="VFC74_08120"
     tRNA            complement(19948..20024)
                     /locus_tag="VFC74_08120"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19988..19990),aa:Pro,seq:ggg)
     gene            20256..20924
                     /locus_tag="VFC74_08125"
     CDS             20256..20924
                     /locus_tag="VFC74_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014793542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK25339.1"
                     /translation="METKLLLVEDDLFLQDGLCELLQREGYSVNCAATYKEALYFAAK
                     HTYSLIILDITLPDGSGLELCEQWRLHGKDTPILFLTARDDEIQIVRGLDAGGDDYVT
                     KPFRLLELLSRIRALLRRNHPSFYQIGELQIDMQKMSALYKGEPLYLTPTEFQLLAVL
                     IRNSGKVLTRKILLQTIWDDGGHFIDDNTLSVHISRLREKIGSSYIRTVRGIGYRWED
                     KPCS"
     gene            20915..21895
                     /locus_tag="VFC74_08130"
     CDS             20915..21895
                     /locus_tag="VFC74_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HZK25340.1"
                     /translation="MQLISLLSGILAALGAAIFFYSSSLIKWGGLGVLMLSMGALCLS
                     VSVQRRQEKEVTRLHRQIMDFLTSQDKIPNFSVNDDLFAVLENSVVELENKLLLEHDN
                     TRKTSKRNADFITDVSHQLKTPLTALKLYCEMDQTQNFGTHSQKQLVLIERMEHLIYS
                     LLRLEKLRADVYELQFAKHDLSQLLEQVWDELRLLYPKKKINIVGTATLRCDAYWLNE
                     AMMNVLKNACEHTATDGQIQVLLETTEAAVTVSIEDNGGGIAAAELPKLFQRFYRTAQ
                     TKASGGAGIGLAITKTIVEKHHGTIYAENMGPGLRMTMCFPILDGVLAAG"
     gene            21970..22656
                     /locus_tag="VFC74_08135"
     CDS             21970..22656
                     /locus_tag="VFC74_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25341.1"
                     /translation="MQILQCQNLCKDYAIGENKVHAIRDISLAFEQGEICAIRGPSGS
                     GKSTLLHILGGLEYPTSGKVHYQNEELFAYNDNQLSILRRRRFGFVFQAYNLVQELTA
                     SENILLPVMLDKKMPDEKYLAMLIELLKLDDRLDHLPSALSGGQQQRVAIARALANKP
                     SVLFADEPTGNLDGKTGREVLSLLKYASKEMGVTLIMVTHDLHIAEQAERIILLEDGL
                     ITQDSGQREA"
     gene            22660..25461
                     /locus_tag="VFC74_08140"
     CDS             22660..25461
                     /locus_tag="VFC74_08140"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HZK25342.1"
                     /translation="MKSTFSLNAIAFGNLRQRRGQYVLLVIGIVLAIYFVATMLLFAS
                     TMFISLQERQFQRLGEQDAIIFNTRETPLSELTERGVFAEYGVAAIQGYVLPDGQNSA
                     NGFSVAVFDDVALQLARKDVLQGRLPVQAGEIALEQSMLARLRTTAAVGDTITLTLQI
                     PDSVSFMPETIIKDYKLVGILTDKLVYLEQRGASLPAYYDYPAGVLSAAEPLTVGGKE
                     VVNVYGRYLPDARESYTKMDTFCAEMGLLGKDGRPPYDIMHYQLFDSSYNNSDIVTTY
                     VILIIIAIVLVAAACLGIINAFAANLEARKRQIGLLRAVGATKKQIRQIFGKEALLLS
                     LFSIPLALVLAYLTVWGITTAMGDSFLLRPNLLILISVAAAGMLCVKLAAAIPLNTAS
                     QVPPMQAIRNVELSRKIKISKVASKKSFAVPHLLAQRSHTLYSNKQAAITAMLAVSIL
                     LISLAVLYVPSILEQATFNYGSDFLLLDYRYDDDWLMAYNFHTPGITEQDRQDTAALP
                     TVAKVTGEKKLSVKILSNTITPYVTNLWEFEYLLAEPRHEDNSEYAEASRVFKALQHQ
                     SYLNSKEKYGYMQDYLTVSCYATEADVVQRLTPYVASGRINLDKLASGEEILLIAPPE
                     YGLYEEQYNDGGWTRRLDYIPDPDVDYQGIYQNDMFQAGDSLSISLLYSDISEQQHAD
                     GPQQYNKDGTRQLPEDAVRVDKTVSIGAILQPLVDGKYLDSNFSFMHTEIGDILTTTT
                     GLQALGFDVPYASLNIMLAASPDEVMEEYLTANLSGIAARTSGAEFKSYIAIARENRK
                     MAYGIVIAVYAVLFLFFAICTSMINNALSARIRASKREIGTMRAVGASEQEIMLTYQW
                     QLSVMFCWGTIIGMVAQLVVCTWILSIDPLWGRVRPTPPVWQPLLFVALLLGICSLSV
                     RAKVGSIVKDSIIANIREL"
     gene            25473..26873
                     /locus_tag="VFC74_08145"
     CDS             25473..26873
                     /locus_tag="VFC74_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25343.1"
                     /translation="MKKNLFLLVFVLFICTAPLVSAEETTDLKNDNLTPAIGAAGPAL
                     SIDSVHKYEGMGKSYAEGYLPAVSENKAMVVLPLLAPEVRDNLTVTVNLGTPADTPFV
                     FKNYQKQFTSQSYVFEEQEVDCFLIQFALPLSAERLNGTYPLILTVKGNTADGEAFAE
                     DFTLYLTITDGINPLAPTPEPAPAPSSQPKLMVESFALSQDYLAAGEQAKLKVTLRNT
                     SSSQSVKNIKLSFTENSGEILPVATGAKYCKQISKGSTYTWNFEVSATTTAQSKPHPA
                     TITMEYEDNRGNAVSGADQIIIQVRQPVRLEYEEPTLPTRVTQGDTPSFSMTLMNLGK
                     STIFNALLKFELPGLSSGGSVLVGTIPPGETAVGRANFRLDTDALGDVSGKLCLSYED
                     EYGEHYESEIPLATTIEKRLELAAPADKKETASSSFPWTIAIVSGCIVLIALLSIFGS
                     RWLQQKRARAEDEMRL"
     gene            complement(27034..27558)
                     /locus_tag="VFC74_08150"
     CDS             complement(27034..27558)
                     /locus_tag="VFC74_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HZK25344.1"
                     /translation="MRKRNWFVLLTAVGTLYYLSSIPSLRVLPVLRQINNLLKYFDLT
                     ISKLAIKIATHLPTQLAPAKTLTADFLNYARRNPVIIEFLLRKAAHVFFFFVITLAFF
                     LLLRNYFRQPWQAIVSSFLGGTIFAILDEFHQGFVMGRHGNYVDVCIDMVGVLTAVFL
                     LVLSFWLTNQHRKA"
     gene            complement(27731..29209)
                     /gene="spoIVA"
                     /locus_tag="VFC74_08155"
     CDS             complement(27731..29209)
                     /gene="spoIVA"
                     /locus_tag="VFC74_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage IV sporulation protein A"
                     /protein_id="HZK25345.1"
                     /translation="MVKFDLIQHIVERTGGDIYLGVVGPVRTGKSTFIKKFMELVVIP
                     NIAEPADQERARDELPQSSAGRTIMTTEPKFIPDEAIEIKVRDNITMRVRLVDCVGYA
                     VPGAVGYDDDGAPRMVTTPWFDYDIPFEEAAEVGTRKVITDHSTIGIVVTTDGSIADI
                     PRTDYIDAEERVIAELKELGKPFVILLNSARPYSQEALALKEELTDRHDVPVISFNAQ
                     QLVEEDINVIFQEVLYEFPVREVNINLPSWVEVLEDEHWLRNNYNDIIRESVIEVTRL
                     RDVDGMVETLSTKDIVENIVLKEMDLGTGRAVIDIQASEELFEQILSEICGIDIDDQA
                     DLLRILKDSVAAKREYDKIAGALEQVRETGYGIVPPMLEEMTLEEPEIVRSGGRFGVR
                     LRASAPSLHVVRVDVKSEFSPIVGTEKQSEELVNYIMDEFEESPEKIWESDIFGKSLY
                     SVVQDGISIKLDNMPPNAQEKLQETLEKIINEGNGGLIAIIL"
     gene            complement(29567..30592)
                     /locus_tag="VFC74_08160"
     CDS             complement(29567..30592)
                     /locus_tag="VFC74_08160"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046168 - glycerol-3-phosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HZK25346.1"
                     /translation="MKEVTVIGAGSWGTAIAIVLAQNCHQVTLWTRRAELCQQINASR
                     ENIDYLPGVILPDNVVLTSDLAAAVHNKEAIVLVVPSHTVRSIVQQIAPLINNSPIMV
                     SCAKGLEEGTLFRMSEVIEQEMPACKLAVLSGPNHAEEVGRFVPSVTVVSSRERRIAE
                     TVQELFMTHRLRVYTNPDLIGVELGGALKNVIAVAAGISDGLSYGDNTKAALMTRGIN
                     EIARLGMRMGAEVLTFAGLSGIGDLVVTCTSRHSRNRSLGVEIGKGRKLNEIIGGMRM
                     VAEGVRTTRATWDLAQRYHVDVPITEQVYQVLFAERDPHQAVVDLLSREKTYEMEEQV
                     SSKFKDW"
     gene            complement(30589..31203)
                     /gene="plsY"
                     /locus_tag="VFC74_08165"
     CDS             complement(30589..31203)
                     /gene="plsY"
                     /locus_tag="VFC74_08165"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HZK25347.1"
                     /translation="MKTIMAFLAAYLIGSISFGYLAGKLIKGIDIRNFGSGNAGTTNI
                     QRTLGTMPAIIVLLLDISKGLLVVWIARALTGSEIVQMAAGVTAIVGHNWPLFFRFRG
                     GRGIATSIGVLLGVAPLVLLIAASIGVSLIALTRYVSLGSITGALAIPIIMIHFHLPP
                     LYLIFGAGIAMMAIWRHRENISRLLRGTERKLGKKANMGNEVKK"
     gene            complement(31219..32529)
                     /gene="der"
                     /locus_tag="VFC74_08170"
     CDS             complement(31219..32529)
                     /gene="der"
                     /locus_tag="VFC74_08170"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HZK25348.1"
                     /translation="MSMPVVAIVGRPNVGKSTMFNRLIQKRVAIEESTPGVTRDRIYG
                     RVEWTDREFWLIDTGGLTFEEDTISKEINRQVRLALEEAHVILMMVDVRSGPVALDYE
                     VADMLRRAKKPVLLVANKAEASESEAVAAEFYALGLGEPQLASGSHGLGTGDLLDEVI
                     KFLPAGEKFPEDEELLRVAIVGRPNVGKSSLTNKIAGNERVIVSDIPGTTRDAVDLLV
                     EHENQKYLFVDTAGIRRRSKVEEVVEYYSVLRSVRAVEDADVVLMMLDAAEGIAEQDK
                     RIAGIAHEAGRAMIIVVNKWDKVEKDEKTMDAYRDTVRKQLAYLSYAPIVFISALTGQ
                     RVHRLFELINYVGEQHAMRVKTSQLNLLLEDAVAVTPPPTKKGKQLKLYYLTQIRVKP
                     PTFALFVNDPELAHFSYMRYLENKLRETYGFEGTPIKLLVRKRS"
     gene            complement(32534..33844)
                     /locus_tag="VFC74_08175"
     CDS             complement(32534..33844)
                     /locus_tag="VFC74_08175"
                     /inference="COORDINATES: protein motif:HMM:NF016354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HZK25349.1"
                     /translation="MTRGKLVERVEPDSIAEEIGIADGDYVIAVNGIEPADILDWQLA
                     ESGEELLLTIQHQGGELVEYEIEKDYDEALGIVFDSPTLDQLHICQNRCLFCFVDQMP
                     ARMRQTLYIKDDDYRLSFLSGSYISLTNLRKEDFERIISLHLSPLYVSVHATDPELRQ
                     RLMNHRRAGELLAALQHLAAAGIQFHTQVVLCPGLNDGEALEQTIADLYALYPAVQTL
                     AVVPVGLTRHRQGLYPLETSDKAHARQVLDQLQAWQERSLAAHGTRFVFASDEFYTMA
                     GMMPPAAEAYEGYRQLENGVGMVRLFLDDWADQEKNLPAKLAHATEATIATGVSAAVY
                     LAPLVERLNQIENLQLTLLAVENHFFGGEVTVAGLLTFHDLNKVINKVKGRLYLPSVM
                     FKEGRELFLDGYTTEELAAAAKTEVKVVQDVHDFLTMLLGNEMR"
     misc_binding    34009..34263
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34328..35365
                     /gene="aroF"
                     /locus_tag="VFC74_08180"
     CDS             34328..35365
                     /gene="aroF"
                     /locus_tag="VFC74_08180"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HZK25350.1"
                     /translation="MIVVMDLQAAQKEIQAVEDVLQEYGYGCHLIRGEMRVVIGAVGS
                     AQSEIPPALETMPGVEKVMRVIQPYKLVSREVKPDDTVVTAGSLRVGGGTVAVIAGPC
                     AVESQGQLRAAANGVKIHQAAALRGGAFKPRTSPYAFQGLLAKGLTYLREAGEETGLA
                     VVTEVVNPADVEMVANAADIVQIGARNMQNYPLLKEVGQLRKPVLLKRGLAATVEEWL
                     MAAEYIMSEGNYQVILCERGIRTFETSTRNTLDVSAIALAKQLTHLPVIADPSHAGGH
                     WRLVKPLALASLAAGADGLIVEVHPDPARALCDGKQSLTLEHFAELMAEIKAFLPVLG
                     KTLCSGVGNDA"
     gene            35358..36194
                     /gene="pheA"
                     /locus_tag="VFC74_08185"
     CDS             35358..36194
                     /gene="pheA"
                     /locus_tag="VFC74_08185"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: protein motif:HMM:NF008865.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HZK25351.1"
                     /translation="MLKAAYLGPRGTFSEEACRAFCRVTGLILAPSPCASITACAVSV
                     AEGNARFAIVPLENSLEGSVRDTLDLLTTSSRLKILAELDLEIEHHLLTHAAALTEIQ
                     TVYSHPQALAQCRAFLHQQLAHAQVIPVVSTAEAACLAADLGSQAAAIAGQQAASCYS
                     LPILYAAVQDTTSITRFIILGREDENFGQPQKTSLLFAVNNTPGDLYRVLRAFANQQV
                     NLTRIESRPARSRLGDYIFFVDLDGTPQDKAVKAALDEASQETVMLKILGSFPVLTVS
                     DK"
     gene            complement(36200..36616)
                     /gene="tsaA"
                     /locus_tag="VFC74_08190"
     CDS             complement(36200..36616)
                     /gene="tsaA"
                     /locus_tag="VFC74_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HZK25352.1"
                     /translation="MELKRIGIIHSSYTDKGAAPRQGKYSQQVSTIEILPEYAAGLAG
                     IEERSHIVVLYWGDRANRETLTAVPPGRTSEKGVFALRSPNRPNPIALCICKIISVKE
                     NSIKVTGLDALNNSPVLDIKPYIPHLDTDHDYQQNL"
     regulatory      complement(36605..36784)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            36965..38110
                     /locus_tag="VFC74_08195"
     CDS             36965..38110
                     /locus_tag="VFC74_08195"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK25353.1"
                     /translation="MTNYASGRLIAELRKAKNMTQKELAAYLNVSDKAVSKWERGENY
                     PEVSILPQLANKLEITVDELLSGKIAPQTASSADLPEAEQVYAYSCLLEDARLRYNKH
                     TLTAYAILAIGILTGSIILNNILPQVAAVALALTGTLFYYFANIQYQNGRDRFVQFAP
                     AAVTADGTSVTLIKYGNTFISTTAFLLLSIATIAARSPHNLFIQDNYLLIDKIMPFPA
                     LLLIIIINLTMYAYFFNGYSRDRLQKISRGDFVKILSSFCVSLILAALVLSVQLQMII
                     TKGAYNSALVNNVTLLALVFAVLTNFCLLFFNNRRTFKKFLHIFVVSMLQAGLLICAA
                     NQYVVSTMHYDTNVLGLVYTGNIITVVMLALTIFHFAFIQLSRKKYA"
     gene            complement(38396..39193)
                     /locus_tag="VFC74_08200"
     CDS             complement(38396..39193)
                     /locus_tag="VFC74_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25354.1"
                     /translation="MLRAENITLAYNDHRIINNLSLVINKGDLMTIIGINGSGKSTIL
                     KALARNLKPTSGTIYLENQSLFSLNTRAVARKIAMLPQMPQVPDDFTTRDLVSYGRYP
                     YLGWNSRLKSEDQEVVDWAIAETRITDLQHRRVSTLSGGERQRVWFAMTLAQEPEILL
                     LDEPTTFLDICYQFEILELVKKLNSSLSLTIVMILHDLNQAARYSNRIAVLKDGGIYH
                     DGAPADVISEQMLRDVFKIEAKVIADREHNCPFFLPLSSSRKQRQII"
     gene            complement(39180..40217)
                     /locus_tag="VFC74_08205"
     CDS             complement(39180..40217)
                     /locus_tag="VFC74_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HZK25355.1"
                     /translation="MNVAHQVESIKLMRQRNSFRLAVILLLLVLCVLSFLFSVGMGSL
                     KITIPEVIEAIVNKTETTNRQIIWNIRLPRTIVAGLVGICLSLSGGILQGVMKNPLAS
                     PSIIGVSSGAGIAATIILVLLPKHYYLIIPCAFLGSLAATLLIYALAWKGGIKPMRMV
                     LAGVAVSSFLSAWINALMVFFPNRVHGVIDFMVGGLAARSWKHFSLLWPYALVGLVLV
                     SILANKLNVLALGDEIATNLGVNVERMRLVLVALASLLAASAVSVVGMLGFVGLIVPH
                     TVRLIIGSDYRYLLPASTIFGATLVMFCDTVARIAMDPIEVPVGIIMAMLGAPFFLYL
                     LKGGLTRDVKS"
     gene            complement(40233..41216)
                     /locus_tag="VFC74_08210"
     CDS             complement(40233..41216)
                     /locus_tag="VFC74_08210"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HZK25356.1"
                     /translation="MKRKIKFCVYFSLCVVLIMLLFGCEGTANNDYQTPELIRAEDER
                     SITIMDPLGNEVVITKEPKKVVVLMNSLLDLWYLAGGTAVGRVSGTENVPPEAESIEI
                     LGSVGSPNLEKIIAMQPDLIIMTSTMAAHREIKDVFAQNKIETLYCEFYDYQDFFENL
                     DVFTRITGRRDIFETDISQLDDEVKEIVDKTVDERKPKVLILFASSKSVTCELPTAQT
                     GNMVDILGGENIVTDTLAEGDVRVNFSLEKIVESDPDVILITTMGDLEECKARIKEDV
                     SSNQAWAGIRAVKEGNIHYLPKDLFMYKPNVRYPEAFAYLANILYPGVTLD"
     gene            complement(41293..42387)
                     /gene="cobD"
                     /locus_tag="VFC74_08215"
     CDS             complement(41293..42387)
                     /gene="cobD"
                     /locus_tag="VFC74_08215"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: protein motif:HMM:TIGR01140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HZK25357.1"
                     /translation="MQQQAKLHPFGHGGDVKTAAEAYGFDPKEIVDFSANINPLGPPA
                     GLLAFLAEQLATVTAYPDPACRKLVNVIREYYQPRQNIVVGNGAGELIYTLMRILPAG
                     TALIPAPSFNLYAKAAQAAGRAVSYHYLQRKHEFKLQVEQICEQIKQERPALVMLCNP
                     NNPTGCGLSRAEVLAVSEACAQSHAYLVIDEAFLEFSADWQERTLLQMAAEHVVVLCS
                     MTKMYAIPGLRLGFLAAAPQLCDAIVKNRDPWSVNSLAQLAGEFVLRDKTFLEKSVEM
                     ITAQAHELYLALQTIPDIYPFTPGANYILLESLQAASVELQAELLRQKILIRDCGNYQ
                     GLNQRYVRVAVRNHTENQTLIEALCSRKQG"
     gene            complement(42412..42975)
                     /gene="cobO"
                     /locus_tag="VFC74_08220"
     CDS             complement(42412..42975)
                     /gene="cobO"
                     /locus_tag="VFC74_08220"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HZK25358.1"
                     /translation="MTAAVKEVISLQENQGIIMVYTGNGKGKTTAALGLALRTSGHGK
                     KVFMVQFRKSDPTYGEIQAIRKYLPNITVVQSKRSTFRGKGGFDPLADVADAKAVLAK
                     GRQALRSGDYELVIFDEINYALHNELLNLTDVLTMCQERPQHVNLVLTGRYARQEIIA
                     AADLVSEVSLRKHHYQAGIKAQPGIEF"
     regulatory      complement(43062..43238)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(43247..44203)
                     /gene="cbiB"
                     /locus_tag="VFC74_08225"
     CDS             complement(43247..44203)
                     /gene="cbiB"
                     /locus_tag="VFC74_08225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HZK25359.1"
                     /translation="MELLSAFLLDAAIGDPAWLPHPVIQMGRLVRALEKRLRRETASN
                     TAQLVTGAVLVMIVLGTTYVGSKLLLFTFTRFNKYLGLLVIIVLLSTTLAARGLQEAA
                     EKIIKPLRAGELATARYAVGLVVSRDTDELTASEVARAAVETVAENTVDGVTAPLFYA
                     LLGGASLALLYKAVNTMDSMLGHKNERYLYFGRAAAKLDDLANFLPARLTVPVMLLAA
                     ALLRLDVKAAVIAMRRDSHQHPSPNSGFAEATTAGALNVTLGGENKYYGGFTQRPLLW
                     QEGRLANVDDIVTTTKLMRLTAMIFLAIGLIIKALILMLVRL"
     gene            complement(44204..45598)
                     /locus_tag="VFC74_08230"
     CDS             complement(44204..45598)
                     /locus_tag="VFC74_08230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HZK25360.1"
                     /translation="MKRLLIAGTNSGVGKTTITVAIIAALKRRGHTVIPFKVGPDYID
                     PGFHSAAAGQSASNLDSWMLRPAQVREILQRRVPSSAIAIIEGVMGLYDGRKNEGESG
                     SSAQLAKITNTPVVLVASGAKMARSAAALVGGYLQFDPEVNFAGVIFNHVGSPSHYQI
                     LTKAVKDYLNLPVLGWLPKDTKIVIPERHLGLLPTAEAENLPDLFTNLADLAETHFDL
                     DSLLAAAAAAGELPEVTETVFPAQHVPVRCRIAVAQDEAFHFYYQENLEYLQALGAEL
                     IPFSPLNDTQLPTDCHGAILGGGFPELYAKELSANITLREQLKTAAAAGLPIYAECGG
                     YMYLAQSLTTFEEQTYPMCGIFPGHSRMHQGRRALGYVEVRGTANNALLLPQESCRGH
                     EFRWSEMEQAGTNYLYQTEKQQLTGERSYNCYGSYLHLHFLANPRLAQRFITVCAQRR
                     QQEANCSNRGKEAG"
     gene            complement(45595..46239)
                     /locus_tag="VFC74_08235"
     CDS             complement(45595..46239)
                     /locus_tag="VFC74_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HZK25361.1"
                     /translation="MEYIKNPLEIERLSMEIIEGRLHGFTFTELQKPVVKRVVHTTGD
                     STFAALIAFGEQAVDAGTAALLAGEPLFCDVEMARSGLSRIGLQTLGIEPACLIHDAA
                     IAKGAKELGTTRAVAAMTHALNICPTGGIFVIGNAPTALFKLLEVVQAGQAKPSLIIG
                     TPVGFVGAAESKELLTSFAIPWITIRGEKGGSTIAAAIVNALVRLALTQKEAAV"
     gene            complement(46504..46626)
                     /locus_tag="VFC74_08240"
     CDS             complement(46504..46626)
                     /locus_tag="VFC74_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25362.1"
                     /translation="MKSAVLLVGQAAIVVKLMGFTGPTATCSEVATATGTGGSL"
     gene            complement(46623..47408)
                     /gene="cobK"
                     /locus_tag="VFC74_08245"
     CDS             complement(46623..47408)
                     /gene="cobK"
                     /locus_tag="VFC74_08245"
                     /EC_number="1.3.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF014613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6A reductase"
                     /protein_id="HZK25363.1"
                     /translation="MILLMGGTRDARRMVAIIREALPATKIVATVVSTYGAELLRQQG
                     GCEVLVQALDCEQLLQLIKERQIKVLLDATHPFAAAASAQACAAAQAAGIYYLRYERP
                     GCELESTDGVYFAPDFPTAARQAARKGRVVFLTIGTRHLAECLEVLKPEACEVVARIL
                     PVEKSLQHCLSLGLLPKQIVALQGPASAELNAALFREYGADVVISKESGAAGGTPEKV
                     AAAQDCNIPIIIVQRPEPIEEKSTPAEIVAALQKVYRNKENGR"
     gene            complement(47405..48112)
                     /gene="cobJ"
                     /locus_tag="VFC74_08250"
     CDS             complement(47405..48112)
                     /gene="cobJ"
                     /locus_tag="VFC74_08250"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HZK25364.1"
                     /translation="MVGLGPGNEALLTPQAAKALQEANVVVGYSTYIRLIQPLLTEQT
                     VLSSGMKKEIDRAKAAVSTALQGKHVVVISSGDPGVYGMAGLVLEVAPATLPVTVVPG
                     VTAATSAAAVLGAPLVHDFAVISLSDLLTPWQTIQTRLAAAASADFVIVLYNPRSRGR
                     QEHLRQAQEIMLTYRSGSTPVGFVKNIGRSGESSVISTLAELSVEQVDMTTTVIVGNS
                     QTKVVHGKMVTPRGYTV"
     gene            complement(48076..49149)
                     /locus_tag="VFC74_08255"
     CDS             complement(48076..49149)
                     /locus_tag="VFC74_08255"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin 5A hydrolase"
                     /protein_id="HZK25365.1"
                     /translation="MSGAVVALTPRGRELALCLKQQFNYSVYLPHSLLQPDDQACPYT
                     ALRVLLPDLFRQQRKLVLIMAVGIAVRLLAPYLQSKQQDPAVVVLDEHGRFAISLLSG
                     HLGGANALAQQLAQYLEGTAVITTATDGAGLRAVDVIAHQFGLTPEPVSRVKELNAAM
                     LRGEPVTVFTDWPVSVFGEAAGLSFQPLAQLKTAQGQPRAVLTHCQQLPGMCVNELLL
                     RPKNLYVGVGCRRGVAAERIEAAVKTVLKDFNLAAASLAQLASIEQKRDEAGLLTAAY
                     NLKVPIRFFNAAEIQAVQYDYEKSKFVEEIMGVGSVCVPTAILAAQSSKLLVPKQKLD
                     QITIAVAEDESPWLASDRVMRLS"
     gene            complement(49146..49907)
                     /gene="cobM"
                     /locus_tag="VFC74_08260"
     CDS             complement(49146..49907)
                     /gene="cobM"
                     /locus_tag="VFC74_08260"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HZK25366.1"
                     /translation="MTVYFVGAGPGDPELITLKGARLLREANLIIYAGSLVNPAILEH
                     AGPQAQLVDSAGLSLPEIITMIKMAVQQGQKVVRLHTGDPSLYGAIQEQADQLQAAGI
                     DSQTVPGVSSFAAAAAAVGRELTVPEVSQTVIITRLAGRTPVPEKENLQALAAHRASI
                     CIFLSVGMIEGVVAELLTAYPPETPAAVVEKASWPQERVVSGTLDNLAQQVKQAGVTK
                     TAMILVGEFLREEQVKPSRLYAPEFSHGFRAGKDQ"
     gene            complement(49904..50620)
                     /gene="cobI"
                     /locus_tag="VFC74_08265"
     CDS             complement(49904..50620)
                     /gene="cobI"
                     /locus_tag="VFC74_08265"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HZK25367.1"
                     /translation="MTKEIFYGIGVGPGDSGYLTKTAIDALAASQVVCVPKSKAERES
                     VALQTAQPYLAAEVLIEELLFPMTRDTVVLAQHWREAASRVAQLLTEHSVVSFLTIGD
                     PLLYSTFSYLLQFLRKLTPTVKTEIIPGISSINAAAALLQVPLLENDERMAVIPVPVG
                     QEEFVELTRYVDTVVLLKVSAAYDETLALLEKTGFNQEVYLVSRVGGKDEFWTNDPFA
                     YRGQAVDYLSLLIAKRRRNG"
     gene            complement(50617..51219)
                     /gene="cbiT"
                     /locus_tag="VFC74_08270"
     CDS             complement(50617..51219)
                     /gene="cbiT"
                     /locus_tag="VFC74_08270"
                     /inference="COORDINATES: protein motif:HMM:TIGR02469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HZK25368.1"
                     /translation="MPNDWTYCGLGLPDTVFIRGRVPMTKREVRVLTLAQLRLQPGQV
                     VWDVGAGTGSLAIEAALAVPSSQVYAIERNAEGVELLQQNSSRFGTDNLTVVAAEAPA
                     ALVSLPQPQRVIVGGSGGQLTEILTYCGEKLTPDGILVVNVVTPQNMVRTLERLSAEP
                     FTNRDGLYVQASRLQKLGREDYFQAQNGVWIISAQKEETK"
     gene            complement(51212..51832)
                     /gene="cbiE"
                     /locus_tag="VFC74_08275"
     CDS             complement(51212..51832)
                     /gene="cbiE"
                     /locus_tag="VFC74_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HZK25369.1"
                     /translation="MLKICLAGVGPGGEKYVLPVVREQVEAADVLLGGARATAPFLSL
                     QKEIIPLTGKLEGLAERLRKLAALKKIVVLVSGDPGFYSLLAYLRRHFTAAELEVLPG
                     VSSMQVAFARLAEPWQDATLLSAHGRNGEELLPSLLRPGKKAILTDQQWTPGRIAKMI
                     LGAGGSDTGVALCYRLTTPAEEIQRTRLSLLDAATAGDCVMVIEDA"
     gene            complement(51825..52946)
                     /gene="cbiD"
                     /locus_tag="VFC74_08280"
     CDS             complement(51825..52946)
                     /gene="cbiD"
                     /locus_tag="VFC74_08280"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013840946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase
                     CbiD"
                     /protein_id="HZK25370.1"
                     /translation="MLPTFQQADGTVLRAGFTTGTCAAAAAKAAVILLYSEQTITEVT
                     VDTPAGIQLTLPLSSVIRETAGVRVGISKDAGDDPDVTHQVEIFAQATPLPRGEFKLE
                     AGEGVGLVTKPGLAIPPGEPAINPVPRAQIRQAVASVLPAQAGAAITISVPAGVQLAK
                     RTLNPELGIVGGISILGTTGIVEPMSSAAFKRSLVPQIDLALAAGQQRLVLTPGKMGK
                     RNAIENLGVAADAVVVTSNFIGYMLKACAAKRVKEVLLYGHLGKLVKLAAGIAETHSA
                     VADARRETLVAHAALAGLPLSVLQDLMSHNTAEQSAAYLRQHGYQQVLRQCAQAAARR
                     AEKIAGGGVKVGCIFLNLAGDIVAHDLQAAKWLEVEDNA"
     regulatory      complement(53093..53269)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            53446..54591
                     /locus_tag="VFC74_08285"
     CDS             53446..54591
                     /locus_tag="VFC74_08285"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK25371.1"
                     /translation="MTNYACGRLIAELRKAKNMTQKELAAYLNVSDKAVSKWERGENY
                     PEVSILPQLAHKLEITVDELLNGEIAPQTASSTNPPEAEDVYAYSCLLEDAHLRYKKH
                     TLTAYAILTTGILTGSIILNNILPQVAAVAFALTGTLFYYFAQSQYQNARDRFAQFAP
                     AAVTADGTAVTLIKYGSTFIGIAVSLLLILNTIAASTPHNLFIQNNYLLIEKTVPFPA
                     LLLLMIINLLLYVYQYNGYRRDRLLKISRGDFVRILSSFGLSIILAALLLILQLQMII
                     REGAYNAILIKNVTLLALVFAVLSNFCLLFFNKRHSFKKFLHIFVISILQAGFLIFAA
                     NQYVVSTKHYDSNVLGLVYTGNIITVVMLAITIFHFTYLQLSRKKYA"
     gene            complement(54688..55143)
                     /locus_tag="VFC74_08290"
     CDS             complement(54688..55143)
                     /locus_tag="VFC74_08290"
                     /inference="COORDINATES: protein motif:HMM:NF022827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HZK25372.1"
                     /translation="MNYLYLGLTDYYLPAAAASLHLKQLNWDAPPSPLKLCELPYFRA
                     VTKADEGVIRYVGLTEDGEQIYLTTVKKYPEIFVRGIHSLLSIYQVPLSELVIITCVL
                     ENPQVAGWCCFLQRIGWHSAAQQLGTRLVRNRLADLKKLVPHFKTIPCP"
     gene            complement(55140..55604)
                     /locus_tag="VFC74_08295"
     CDS             complement(55140..55604)
                     /locus_tag="VFC74_08295"
                     /inference="COORDINATES: protein motif:HMM:NF022827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3189 family protein"
                     /protein_id="HZK25373.1"
                     /translation="MTVKKIIYHCYGGAHSSVTAAAIHLGQLQADKIPTAQELLSLAL
                     FDRQTAEGHGQLHFFGFDEWGNQIYSVGCRNVGQTMVNILHGVSKLLGIHEQLVFVDT
                     LHCVSLKMRIGGYLSRRWSIISVGRPLVIQGTREAYPKIVELVAQVKVKLAG"
     gene            complement(55624..55818)
                     /locus_tag="VFC74_08300"
     CDS             complement(55624..55818)
                     /locus_tag="VFC74_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25374.1"
                     /translation="MEVTIAKIILAAVTTRKGEHRVAGIPIFYAETDEEMINMCTILS
                     RVMEAVAHDMGNGVYIIVKH"
     gene            complement(55828..56718)
                     /locus_tag="VFC74_08305"
     CDS             complement(55828..56718)
                     /locus_tag="VFC74_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIEGIA family protein"
                     /protein_id="HZK25375.1"
                     /translation="MNKDILTIICGVTMGTIARYWMMRRDFRQYPSYPHATVTHLALG
                     FVAATLGAVAIPAIAAKEYTAVTFLTLAATQFRDVRNMEREMLVSLDQSTLVSRGADF
                     IEGIARTFEARNYLVVFVSLNTSLFTYLYSPLPGIVAGAIALTAVRRFMRGKLIGDIA
                     KVRPAKVHFDGANLFVEDIHIMNLALPEVKKVYEEMARGVVLEPISDNGREILANVGQ
                     RQAIAHDVALLLGVRRDVDTAEFTPLLRRKPETGRVGMVIVPIEPDIECLVAAVQNVP
                     VLESAFVQPLKSRIGRSAAD"
     gene            complement(56724..57932)
                     /gene="spoIIP"
                     /locus_tag="VFC74_08310"
     CDS             complement(56724..57932)
                     /gene="spoIIP"
                     /locus_tag="VFC74_08310"
                     /inference="COORDINATES: protein motif:HMM:NF019097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061783 - peptidoglycan muralytic activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein P"
                     /protein_id="HZK25376.1"
                     /translation="MRRSSFNTFKLTAVLVMVVSLLFFSTQSSFAIDNLIDGIDGDHR
                     SGVYYTMEDNNGKVIMRTAREIVVGDEYISADNNYYRVTSVRGETARAELIEKRTLAA
                     PPQPGLLARVSNMLRSAVPVQKKGEQKTKKIAIYSSHGAESYIDGDGTESKDPGGGIL
                     DVADTLQKKFEEKGIKVVRSKEPHTPHDAGAYQRSRRTVEEALKEQPDALIDVHRDAV
                     PEEEYLEEVEGEKRVQVQLVVGRQNQNRANNQQFAEGIKKQADEKYPGLVKGIFMARG
                     NYNQDMSPRATLIEVGSHTNDKDQAEETMGLFSEVMNDFIYGTAEGEQAASPSPTTSQ
                     GAGGTALRSVLWIIAGLVAAVAIFLLISTGGFEQAKAKLNQFRSREMADLTGSRQDED
                     DSSDGGSEGS"
     gene            complement(57919..58641)
                     /locus_tag="VFC74_08315"
     CDS             complement(57919..58641)
                     /locus_tag="VFC74_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HZK25377.1"
                     /translation="MTIGMLVLVAVAIAIYLGAAQRVLDRLRLTDRAALLFVGAMVVG
                     GFLPDIPLSDNVSINIGGALIPLALAVYLIIKAGTAKEKIRAVVAALVASFAVYAAMK
                     ILPTEPTYNRFLDPFYIFSIIAAVVGYLAGRSRRSAFIAGSMGLLLTDIYSRIEVALR
                     GGSGSMVIGGAGVFDGVVIAGILSLLLAEIFGETRERLQGGPAEDRPSSLRKGLSEGE
                     FSEELSHDENSLTKEENNHEEE"
     gene            complement(58842..59870)
                     /gene="fni"
                     /locus_tag="VFC74_08320"
     CDS             complement(58842..59870)
                     /gene="fni"
                     /locus_tag="VFC74_08320"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HZK25378.1"
                     /translation="MTRQVRKLEHLHYAVQLDLASADFSDIELIHNCLPETALQALDL
                     TTTVAGLTLAQPLLLNAITGGVEAAEAINHDLALAARSCGLPMAVGSQMAALENQRYQ
                     QTYRVVREVYPQGIIFANIGANAGLTAAQQAVEMVAADALQIHLNVPQELMMAEGDRD
                     FRGWRQRLAAIVKAVSVPVIVKEVGFGIAKEQAAQLQDIGVAAIDVGGQGGTNFLQIE
                     SARTKQQISAELLQWGIRTPLTILEAKAGAPAVDIIASGGINSGLLAAKALALGAQAV
                     GSAGLALRILQKAGPEKLVASLQKILTELKMVMLMTGCSSIRELQEVPLVITGQAREW
                     MTARGLSR"
     gene            complement(59874..61901)
                     /locus_tag="VFC74_08325"
     CDS             complement(59874..61901)
                     /locus_tag="VFC74_08325"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF000907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-3-methylbut-2-enyl
                     diphosphate reductase/30S ribosomal protein S1"
                     /protein_id="HZK25379.1"
                     /translation="MAEKAGFCGGVKKAVALALATAAQGPIYTLGPLAHNESLIDRLA
                     EQGVRQVDDLTSVPSGATVIIRTHGVRPEVLQAAAARGLNIINATCTFVQKLQQQVAA
                     LVEAGQQVVIVGTREHPEVQGVLGWGHERPFVVQTAAEVEQLPGLDSTRPVAVVAQTT
                     QQVRILEEIVEKLKELVPVVHIYNTICNATALRQKAASELAQKAEAMVVIGSKKSSNT
                     QKLAAICRAAGTKTIEVTDADSLDTAQLQGLRTVGVTAGASTPDWLIKEVIGKMENEK
                     NLEAEVQEEQVEVEEVPMDQVMRDFSVGDVVQGTVEQVSDDEALVDIGYKSEGVLPRQ
                     EVILQADQTLSAVLQTGQEIEVVIQDMDDQEGKVTLSRKAIDRQKRWEELEEAFNQGT
                     VLTGTVKETVKAGMVVDLGGGYDGFMPGSLVDMRYVPDFTVFLNQEVSFKIIEMRKEK
                     EKLILSRKQVLEEETQVKKEEILNSLQPGQIIHGTVKRLTNFGAFVDVGGIDGLVHIS
                     EISWHRIETPGEVLSVGEEIDVKVTEVIPERERIGLSLRKAQPDPWTQIDKQYQPGDV
                     VEGKVTRLVDFGAFVELIPGVEGLVHISQLANYHVKQPSDVIQKGEVVKVKILDINTD
                     AKRVSLSMREAAPRPKREPQQRAPQQHQDSGTGLTLGDVFGDLFDGMNKDK"
     gene            complement(61931..62512)
                     /locus_tag="VFC74_08330"
     CDS             complement(61931..62512)
                     /locus_tag="VFC74_08330"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HZK25380.1"
                     /translation="MAYQIFRRFFAVIFAIFFRWDVQGRENIPQEGPFIMCGNHFSWW
                     DPPLLGAITPRFARFMAKEELIKTPILGTVLKAIKVFPVKRNSADRRAIKTALETLKA
                     GHGLGIFPEGTRSHTNELLTPQAGVGLIAAKSEAPVIPIGIAGPYRLFHPIRVRIGTP
                     LYFPEYYGVKVKGEELAKIAQRIMQEIDRLRKS"
     gene            complement(62529..63191)
                     /gene="cmk"
                     /locus_tag="VFC74_08335"
     CDS             complement(62529..63191)
                     /gene="cmk"
                     /locus_tag="VFC74_08335"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HZK25381.1"
                     /translation="MKPRIAIDGPAGAGKSTVARAVAKRLGLAYLDTGAMYRAITVAG
                     LRRKIDFQDQQALAVLAAQADLDVQYNDEGQNILFLDGENVSETIREPQVSRHVSLVA
                     RCPGVRAVMVQKQKEIGQRGGVVMDGRDIGTNVMTEAEYKFYLTASLAERARRRLAEL
                     LEKGQSLTLEQMKEEIAARDKIDSERECSPLRPADDAIHIDSTELTIDEVVALIVETV
                     TR"
     gene            complement(63279..63656)
                     /gene="aroH"
                     /locus_tag="VFC74_08340"
     CDS             complement(63279..63656)
                     /gene="aroH"
                     /locus_tag="VFC74_08340"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HZK25382.1"
                     /translation="MGCCVVRGIRGATTVSQNSASEIKAATRELLHKMAEVNQIDPAA
                     LVSIIFSATPDLNAAFPAAAARELGWTAAALFDTVEMAVPGALPRCIRVLMHVNMEQN
                     QAVQFIYLREARQLRPDLVSDNT"
     gene            complement(63736..64242)
                     /locus_tag="VFC74_08345"
     CDS             complement(63736..64242)
                     /locus_tag="VFC74_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25383.1"
                     /translation="MDFGRWFQERTSRFERFMLKCVGVMLFLLLLTQALLTRPDFRSI
                     LSLVDRFEGTPVTQQEPDQPAISRPVVDNEQRYLRLSIINDADGTGIKVLVNGETVTA
                     FADGSSVLVAVHDGDQVEVDSKVPTEDVVIAVTSVSEGIIAPIEGKQITFFGQPETVS
                     FVSVGKVD"
     gene            complement(64289..65527)
                     /locus_tag="VFC74_08350"
     CDS             complement(64289..65527)
                     /locus_tag="VFC74_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014183606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK25384.1"
                     /translation="MKIVVIGGGPAGMMAAGQAAKAGANVILVEKNLRLGKKLAITGK
                     GRGNVTNAAEIEEMIQQYPGNGTFLYGPLYRFTNDQLRQFLLDLGVPTVVERGGRVFP
                     ESQRAQDVVDALTQWLQNQGVQVQLGKQVTKLIITEGGEIRGIVCDEEEIAADAVIIA
                     AGGASYPATGSTGDGYRLAQAVGHTIVPTRPALVPLETEENWVRELTGVALKNVTAKL
                     TVKGRVVAEEFGEMLFTHYGVSGPIILTISREAVNALVKKQKPQLHLNLKPALTREKL
                     DARLLRDFENNIRKQFKNSLNDLLPQRLIETIIMLSGIDPELPVNQITKAQREALLTA
                     LTDLTLNIKSMRSLREAIVTAGGVAVKEIQPTTMASKLVGGLYFAGEVIDIDGNTGGY
                     NLQAAFATGFVAGLSAAQQA"
     gene            complement(65673..66173)
                     /locus_tag="VFC74_08355"
     CDS             complement(65673..66173)
                     /locus_tag="VFC74_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25385.1"
                     /translation="MLKPGFERQLPLTEPRPEEVTEMKNAEKIRARIRFDYRGKARPS
                     RFFFGGKGTEEVAAELREQQAALWRNVPVQGVFVENIELGEIYSVYDEEVDDEVAFAP
                     LELEVTADSFNFLIRFAVRDEFRRLKIVEPSGLQMSVPEMEQIFFEVHSQAKNQWRQK
                     MKRYAD"
     gene            complement(66238..67611)
                     /locus_tag="VFC74_08360"
     CDS             complement(66238..67611)
                     /locus_tag="VFC74_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007785354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25386.1"
                     /translation="MGNNIIRARIALDSLTVLRGYLTDPVIAKYQRLLQSLRTTSFDG
                     YVWLNNYADFYYTLLQANKVPSLKEYILEKLLFCDTVFSQRCEHQAYDELTPRIVEAM
                     RHDVSRLEEIAALTASGLKAAALERTSNSSYLTTALQELPEWDTSSPAVSRHSAAQEL
                     MQKFQTADQWVDCLPDLTVFYQQHGSGDFARYIGFRWESNCLQGITAVDPISFAELIG
                     YQTERQQVIDNTLMLLRGYPANNLLLYGDRGTGKSSTVKALLNEYGEQGLRMIELPQT
                     RLADLPLVLREVKGRKQKFIIFVDDLAFADNEERYTALKAVLEGGLESRPQNVVIYAT
                     SNRRHLIKEKFSERAGLQSDDLHDQVRAADTMQEKLSLADRFGITITFLSPDQRKYLE
                     IVEGLAQQRQLQVETSHLRSEALRWERWQNGRSPRTARQFIDWLEGQLSQNLSGENGK
                     NSRISVS"
     gene            67787..69703
                     /locus_tag="VFC74_08365"
     CDS             67787..69703
                     /locus_tag="VFC74_08365"
                     /inference="COORDINATES: protein motif:HMM:NF012927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK25387.1"
                     /translation="MYEMFLSPGKIGKLTLKNRAIFPPMGTGYVEHEEPKEQLIDYHV
                     RRVLGGCAMNIVEITAVHETSKSPTNLAIYDDKFIPGLARLAAAIKEAGGIACLQLWH
                     GGRQTSGKPFGGQPVAPSAVKNDFINEEPRALTLEEVKELINCYGEAARRAQEAGYEA
                     VEIHGAHGYLIDQFLNPYTNKRDDEYGGSLENRARFGVEVIKAVRAKVGQDFPVLFRL
                     SAQENVPGGIKLEEGIAAAKLYEQAGLDALDVSQGCYDALPYTVPPYFLPQKVNAANA
                     AQIKQQTKVPIIVAGRINHPELAEEILQSGMADFISLGRPQLADPDFVNKTAHDHADE
                     IVRCIACNQGCVGRLFKGLGTSCIFNPATGHEREVVIQPAPTKKKVLIIGGGPAGLEA
                     ARVAQERGHDVVLFEKDVGLGGQLVVAGRAPHKEIFAESAIHLGYRAFKAGADIRTYT
                     EATEERIRNVQPDVIIIASGADPALPKIPGGDQPHVYEARAVLNADQLIKANQVVVVG
                     GGLVGLEVMEVLLAQGKHVTVINRGAEVGKELETYIKPYVFGIIEQEQVPILTNSSCV
                     EIGDGFVVVEKDGQQEKVKCEAVVMAVGAKSNTKVVELVARLGYEHYVVGDAQQPNRI
                     LEAIWGANEIARTI"
     gene            complement(69797..70408)
                     /locus_tag="VFC74_08370"
     CDS             complement(69797..70408)
                     /locus_tag="VFC74_08370"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZK25388.1"
                     /translation="MKLIWVRHGETLWNRQYRLQGLSDVALSELGCRQAEQLAAAFLE
                     KPAHIFVSPLQRAQTFAAPLATRFELPLQLDDNLREMSFGQWEGLSYAEMDQAMQKQF
                     AAWCNDPVTNTPPAGEGAAALAARVDDFLQTLVARLEQEETAVIVTHGGVIRTAVTLA
                     MQMPAAAAGRLQIDTASLTTLYNYNNCWYLAKLNDTSHLQERV"
     gene            complement(70452..71186)
                     /gene="cobS"
                     /locus_tag="VFC74_08375"
     CDS             complement(70452..71186)
                     /gene="cobS"
                     /locus_tag="VFC74_08375"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HZK25389.1"
                     /translation="MKRFFVALRFLTVYPFGGDTEVTTDDLVGSATYYPIVGALIGLT
                     LYGFWLWANTVLSTLLAATLTVTLWELISAGLHLDGLMDTADGLGVRGNLEKRLEVMK
                     DSRVGAFGAQVLVLSMLLKVFAVASLAADARLLIIAPLVGRTVMVALMATCDYARPSG
                     GLGKIFVDELGTTQLFLAILLCLVLGFAAWGWQMLPLTLALIVVFAILRYFFKLNFGG
                     VTGDILGAVCELFELAVLLIAPLLFL"
     gene            complement(71270..71854)
                     /gene="cobU"
                     /locus_tag="VFC74_08380"
     CDS             complement(71270..71854)
                     /gene="cobU"
                     /locus_tag="VFC74_08380"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: protein motif:HMM:NF004469.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HZK25390.1"
                     /translation="MDMEKLGKLVLITGGARSGKSGLAEQLAHTAGGPATYIATAAVL
                     DEEMALRVALHKERRPPHWSTVEETKDLAGALERVPAGTVTVIVDCLTIWLTNQLLAQ
                     YEENMSDEKYLDIEKQIQTELARFCTAALNKPFRTVVVTNEVGSGIVPDNYMGRVFRD
                     MAGRANQQVARAADSLWLAVSGYPLCVKAYQNVE"
     gene            complement(71892..72725)
                     /locus_tag="VFC74_08385"
     CDS             complement(71892..72725)
                     /locus_tag="VFC74_08385"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK25391.1"
                     /translation="MSTVIMVQGTSADAGKSILCTALCRLLAEEGWRVAPFCAQTIGA
                     ETVDLPKGQISWSQYLQAEAAGVTPSVEMNPLLLAPLSEDSFQLTAQGVIKGELTRQE
                     LLAVSDTALALVLDALQVLRSHYEVVIIEGAATPVELHRPELMLPNMGLAKALDAPVL
                     LVSDIDRGGMFAYVAGTLALLPPEQRQLIQAVVVNRFRGFRDKLQPGLEMLEEIIHKP
                     VIGVIPHLNGLALPPQAEAASAATKEKREQSYQLLAQTLRENLDLSFIRKLLPAVRSK
                     Q"
     gene            complement(72722..74263)
                     /locus_tag="VFC74_08390"
     CDS             complement(72722..74263)
                     /locus_tag="VFC74_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase"
                     /protein_id="HZK25392.1"
                     /translation="MPKTSKQAVTIMVQGTASHVGKSAIAAALCRIFARAGYRVAPFK
                     SWNMALNSYVTSDGGEIGRAQGEQAEAAGIEATVDMNPILIKPSAPGEAQVIVRGQVY
                     GMMGHTHRTQQEYHEFSLQVIAEALDRLRQEYEIIVLEGAGSPAEINLRQQDVANMQA
                     AALAQAPVLLVSDLDRGGAWAASVGTVMLLPEEDRQRVAGFIINKFRGDSSLLAPALK
                     EVTAQTGLPVFGVVPYLEEAYLAEEDGVALDDTLSSTVKGEGCLNICVLHLPHIANFT
                     DFDALRSEEDVSLSYTYDGLDLAGADAIIIPGTKNSMRDLLFLQQLGLAEAVIARARQ
                     GLPVIGICGGYQMLGEELSDAEGSEFAGQATTLAGLGLLPVTTSFQPQKTVGRASAAA
                     QLPFAQGAAVTGYEIHMGQTKRQAHCPAAFVLADGKEDGAISPQGNVWGTYLHGVFDR
                     PGFRRAWLNKLRQRRGWQPLGESAVEDRAQRFDALADAVENSLDLSALYRLLGLAAQK
                     GEHQA"
     gene            74388..75059
                     /locus_tag="VFC74_08395"
     CDS             74388..75059
                     /locus_tag="VFC74_08395"
                     /inference="COORDINATES: protein motif:HMM:NF014171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA C-terminal domain-containing protein"
                     /protein_id="HZK25393.1"
                     /translation="MFVELPLLFLVFALLYLIVEAATTMLMITGLDQHTARFQAISII
                     TGTGYTTSESELITRHPVRRKIAMFLMIVGSISLAVVISILVRLLSSNVSGVELFLTI
                     ALLLILTLILRSRPVLLYFDNYLEKQMLKQPYIHKRTVEELLKIDEHFSIAEIQLSQN
                     APWLDQTLGRARLRDLGVLVLSIRRRDGNLIRAPQGADVLREGDILLVYGRPRYISEI
                     IELAT"
     gene            complement(75003..76313)
                     /locus_tag="VFC74_08400"
     CDS             complement(75003..76313)
                     /locus_tag="VFC74_08400"
                     /inference="COORDINATES: protein motif:HMM:NF010068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HZK25394.1"
                     /translation="MRIEIQDISFTYGNHQVLEGTTFHVSSGKFVGIIGPNGSGKTTL
                     LKVLSRILQPTAGCVRIGEQSLSELTQKELARTMAVVSQDTNVGYQFSVEDVVLMGRA
                     PYLGRFQSESLADYEIARHALEVTGCTHLRDRSILELSGGERQRVTIARALAQQPKVL
                     LLDEPTSHLDIGYQQEILDLVKRLSTVEGLTVVAVLHDLNLAAYFCHELVLMHQGKIL
                     ACGQPTDVLTADNIEKVYATRVLVTPHPVFGTPQISLLPGTREQNKHGENHQIHVIAG
                     GGSGGEIMRDLCDAGYKVTAGVLSVGDSDWEAARALSIPVISEAPFSSVSPERHQENL
                     AMLETAQAVILTEIPLGHGNLLNLEAALQAAAAGKPVYMIEEKPAQNRDFTGGPGQEL
                     LTKVQQQAQSIREKTELYRALAEQFSSSGQLNDFTDVTRPAVDQ"
     gene            complement(76321..77355)
                     /locus_tag="VFC74_08405"
     CDS             complement(76321..77355)
                     /locus_tag="VFC74_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HZK25395.1"
                     /translation="MKTRNKYLLYTLLLSALVVSVILATAVGAVAIPPRETFQIIISR
                     LTGLWQSSDIPATTQAIIWNLRLPRVLLGLLVGASLALAGVSFQGLLRNPLADPFTIG
                     VSSGAAVGATIAMLLQQTYQISFTQMLPIFAFAGAFLAMFFVYNLARIGGQVPVVTLL
                     LAGVVVSSFLSAVISLLMVFAGEDMQGIFFWLSGGLLMRSWSEIWFVLPYLLIGSIIL
                     FTYARDLNVLLMGEEAALTLGVNVERTKRVLLVTASLITAAAVSVSGMIGFVGLIIPH
                     AVRIVTGPDHRVLLPASALVGATFLIWADVVARTVMIPKEIPVGIITAFVGAPFFIYL
                     LRKKKSEIRL"
     gene            complement(77358..78287)
                     /locus_tag="VFC74_08410"
     CDS             complement(77358..78287)
                     /locus_tag="VFC74_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HZK25396.1"
                     /translation="MRKRNWLVVMLLVVFTLTLVAGCGQENGGEEANGDTDTGKTYPL
                     TLTDAAGREVTLAAKPERIVSIMPSLTEILFALDAGERVVGVTTYCDYPAAAKEKEVI
                     GDLYNLNPESILALQPDLVLVGKSETLQQTFDFLEQAGTPYFVVDPQTLDEIEESIVT
                     VAKLIDNQAAGEALVTQLKADRLALETKVAEIPAEERAKVFVLLDTNVLYTVGEGTFL
                     SEMITTAGGNNLASEIDDAYFQVSEEAFFELDPEVIICTFPMRDEVLARETWQDLSAI
                     KSGKVYDVDANSVSRPGPRYVQGLELLYEVFAK"
     regulatory      complement(78463..78638)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(78695..79423)
                     /locus_tag="VFC74_08415"
     CDS             complement(78695..79423)
                     /locus_tag="VFC74_08415"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HZK25397.1"
                     /translation="MAVRLQKYLAMAGVASRRAGEQMIREGRVKVNGQTITEMGVTVD
                     PAVDQVEVDNRLVKPVEKKEYILLYKPIGYVTTLKDPYGRQTVQDLVKDVGTRVFPVG
                     RLDLDTSGLLLLTNDGELANRLTHPSFGVEKEYLARIHDLPDSEALTKLQKGVKLDDG
                     YTAPAAVRLIKGGRPTSTLSLTIKEGRNRQVRRMLEAVNHPVVSLQRVRVGPLTLTGL
                     KKGQWRRLNGAEVKALKRQAGVKS"
     gene            complement(79562..80098)
                     /locus_tag="VFC74_08420"
     CDS             complement(79562..80098)
                     /locus_tag="VFC74_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZK25398.1"
                     /translation="MLQVIVQTAAAWVIPGFIFIVLVYAVVKKVDVFDAFVEGAQEGF
                     ATSVSLIPYLVAMLVSIGLLRASGALELLIKLLSPLLTRFNVPGEIIPLAIMRPLSGS
                     GALGITAEILNAFGPDSFIGRLASTMQGSTDTTFFVLTVYFGSVGIRKTRHALTVGLL
                     ADLAAFIASIVICTLVFH"
     gene            complement(80104..80700)
                     /locus_tag="VFC74_08425"
     CDS             complement(80104..80700)
                     /locus_tag="VFC74_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZK25399.1"
                     /translation="MINLIWFAMIAIAFVTAALTGSIETFTDALFSSAEQAVSVVFSM
                     VSIMTFWLGMMKIIEKSGFIEWIVKLLRPLSRLLFPRLPRNHPAMHAILMNMSANLLG
                     MGNAATPFGLKAMQELQSINEDPQTASDEMCTFLAINTSALTLIPTTVVALRAASGSL
                     YPTEIVGTTILATLCATTTAIILDKLIRGLTRRKRKRR"
     gene            complement(80728..81843)
                     /locus_tag="VFC74_08430"
     CDS             complement(80728..81843)
                     /locus_tag="VFC74_08430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZK25400.1"
                     /translation="MRKFIIVLLVSLLLLQPGSGWAAEPPQVTARAAVVLDQATGRIL
                     FSKNAHEQLPMASTTKIMTAILAVESGKLAEVVTVSEYASLVEGSSVDLEAGEKKTLE
                     ELLFGLILRSGNDAAAAIAEFLAGSVEEFAEQMTEKAHALGAVHTNFMNPHGLDHEEH
                     YTTAYDLGLIAVHAMTLPKFREVAATAEKKISWTGRSYDRLLSNQNKLLTMYEGAEGI
                     KTGWTTPAGRCFVGAAARDDWRLVTVVLNAPEMWEDTTDLLDFGYAHYQRKTIVKAET
                     PLKTAAVRKGAAQKISLVASEDIMLPLKDNEADLLRYAWEVPEEVQAPISKGEQLGTL
                     RIYFGTQQVSEVPLVAAAEVESKGLFASLRKFFQRIF"
     gene            complement(81939..82376)
                     /gene="ytfJ"
                     /locus_tag="VFC74_08435"
     CDS             complement(81939..82376)
                     /gene="ytfJ"
                     /locus_tag="VFC74_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014826779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HZK25401.1"
                     /translation="MQEHPIQGLMKTAMENIKEMVDVNTIIGDPVQTQDGTTIIPISR
                     VTFGFAAGGSEFDNDLEEEKKNKKAEDVNYLPFGGGSGGGVSVQPVAFLIVSHDGHVK
                     MLPVDRQVMFDRLMDMAPQIINQVQGMLQKEPPADAQLNHYQI"
     gene            complement(82379..83026)
                     /locus_tag="VFC74_08440"
     CDS             complement(82379..83026)
                     /locus_tag="VFC74_08440"
                     /inference="COORDINATES: protein motif:HMM:NF022615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2953 domain-containing protein"
                     /protein_id="HZK25402.1"
                     /translation="MKWILTLLPLYLSGMLLLQLIPVRINLFFVRDNKDDFLNIRVNT
                     FFSLLRFNVEVPLIQQDTPFDVTLEAELKAGEDKLVGEKKEKISVFDLDYEKVKLYVA
                     WIKDNKDMLSYLSNFYRQAMTIEKFVLRVHVGLNDAALTGLLTGLFWSVAVIFVPALQ
                     KRLRFKERPVLAFKPDFSPQTVAKVYFDAVVKLRIGHFMLGSLLFMSTIIRGGKD"
     gene            complement(83153..84025)
                     /locus_tag="VFC74_08445"
     CDS             complement(83153..84025)
                     /locus_tag="VFC74_08445"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK25403.1"
                     /translation="MNNKTVSLVLLLLLVATVIGAVVINQRSEVVATVNGEKITKNDL
                     YEAFVANGGGTVLDQLISERVVAQAAAKSGIKVTDAEVQQEIDQLIEKSYYGMKEYFD
                     QALTQYGITEETLKENIRNELLLSAIVRSKIEVTEAEVEEYFKENQAEFNIPEKVDVR
                     HILVETEEEANEMLASLKAGEDFAELAKEHSQDTGSASKGGALGLAVRGQFVKEFEDV
                     AFALPVGEYSEPVKSEHGYHVIEVLERQEAQEVEFSAVKEQVEEALMEKKVSESMQEE
                     YSKLYEAAKVEKKL"
     gene            complement(84208..84726)
                     /gene="scpB"
                     /locus_tag="VFC74_08450"
     CDS             complement(84208..84726)
                     /gene="scpB"
                     /locus_tag="VFC74_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HZK25404.1"
                     /translation="MEIKPLVEALLFVAEEPLPPAKIAEIIGAETKDVRETLKLLQQE
                     YASSEHGLQVMEIAKGYQFSTKPDLSPVIEKLFKGEKSYTLSQAALETLGIIAYRQPV
                     TRVDIETIRGVKVEGVMNTLQRRRFIRTVGRKDAPGRPILYGTSKEFLKYFGLKDLSE
                     LPRLTDNEEKQF"
     gene            complement(84744..85475)
                     /locus_tag="VFC74_08455"
     CDS             complement(84744..85475)
                     /locus_tag="VFC74_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HZK25405.1"
                     /translation="MEYYVNLPTFEGPFALLFHLIEKAEVDIYDIPVAEITAQYLETL
                     QLMQKLNLEVASEFLVMAATLLKIKSKKLLPSSIGDEALDDEDSMDIETQEELIQRLL
                     EYRHFRAVAQELRAYETAQKRVFVRSLTGEKVVLVNPEDDFVISGVSLPALMQAFQRL
                     LAEHERQPLEVEADDFSVEELMVQLVSQLQGQKDGLSFSGLFTETAGLSEIILTFVAL
                     LELVRLGKLSVWQSNPFGELVVLLK"
     gene            complement(85608..86597)
                     /gene="trpS"
                     /locus_tag="VFC74_08460"
     CDS             complement(85608..86597)
                     /gene="trpS"
                     /locus_tag="VFC74_08460"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HZK25406.1"
                     /translation="MEQKTIFSGMRPTGRLQLGNLLGALDNWVKLQDDYRCLFGVVDW
                     HALTTAYEDPSGLQENTYQMVMDWLAAGLDPGKSIIFRQSDVTGHAELHLLLSMITPL
                     SWLERVPTYKDQIQQLKEKNLGTYGFLGYPLLMAADILLYRAEAVPVGEDQIPHLEIT
                     REIGRRFNFLYKTNFFPEPETILNVVKMLPGIDGRKMSKSYGNTINMSEAPDEVRKKV
                     QQMVTDPGRVRKDDPGNPDICAVYTFHSVFNTDELASVEGACRRGEIGCVQCKKQLAD
                     RLVAFLEPIYERRQALAGQRDYIRDILLDGAKRASAIANQTMAEAREIVNLKL"
     gene            complement(86611..87261)
                     /locus_tag="VFC74_08465"
     CDS             complement(86611..87261)
                     /locus_tag="VFC74_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HZK25407.1"
                     /translation="MPISNLISRFVTSGLFYRIPALLLALTLHELAHGLVAYKLGDPT
                     AKQQGRLSLNPLKHIDPLGLLALWLVGFGWAKPVPINPLYFHGDRHRGLLWVSLAGPA
                     TNFLLAFFTALLLMLLPPLRSTYILPFMQLLFVYNIFLGVFNLLPIPPLDGSKVLFYF
                     LPRSAAYKFAQLEQYGPLILMLLVFTRTLSRILYPVAGVVSNVITTIVVLLTGGGI"
     gene            complement(87263..89899)
                     /locus_tag="VFC74_08470"
     CDS             complement(87263..89899)
                     /locus_tag="VFC74_08470"
                     /inference="COORDINATES: protein motif:HMM:NF025112.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HZK25408.1"
                     /translation="MQVITTHLNADFDGLASLVAATKLYPGAQAVLPTSLNKNVQEYV
                     SLYKDQLPFKTSKELALDEMKQLVITDTRQRQRLGSFAKYLDQLTEIHLYDHHPESAD
                     DLAAQYMVVGNVGATITLLWEKISTQGITISPFEATLFALGLYEDTGGFTFENTTERD
                     VAALHQLWQVGVNLRVVNQFLKQPLNDQQRDLLDELLDATEYYELRGVRVGIATAANG
                     DYVDGLGSLTQKLLEIEDVDLMLTAAAMGNKVYLVGRSRVEEINVSDLMAVFGGKGHR
                     AAASAMVKDTTLAAVQEQLIKVLAEEVKPVTTAREMMTAPVRSISPETSIQEAQELML
                     RYGHSGFPVVSEGQLVGIISRRDLEKAMRHGLGHAPVKGYMSRKPVTVAPEMSLREVQ
                     HIMITKDMGRLPVVEGEEIIGIITRTDLLDSLEGSKMKIKQQGESPAFPSAGQDITTL
                     FMERLPQKLQSLLYLIGQTADREQSKVYAVGGFVRDLLLGLETHDLDLAVEPLAIPFG
                     EKLHQVLGGQIKKHEQFGTVTLTMRDDLQIDLVTARQEFYARPAAMPEVELGSLKNDL
                     FRRDFTINTLAVSLNMPHYGQLIDFFHGLSDLESGLIRILYNLSFVEDPLRLLRAIRF
                     EQRFAFQLEETTQALLENAVKTRVLENVSKERLYDELSLIFQEKKIAAMLNRIFELGL
                     DVSIFPGVRYHELLKQRLFTVQEVLACVQRTWLDAKPIPAALYLASLMLDLRFQEARH
                     LCRRLRLPRDTIRRVLNAITVVPQLQEELTTDEELKPSQLEHWLGGQPLETMLLLLVV
                     SKHSLVWSRIYFYWEKIRPIQIEISGHDLEVLGLPPGPAYQEILTAVRAARVDGLVGN
                     KEEELAFVQQQFQRDPPWGKEN"
     gene            complement(90204..91295)
                     /locus_tag="VFC74_08475"
     CDS             complement(90204..91295)
                     /locus_tag="VFC74_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase"
                     /protein_id="HZK25409.1"
                     /translation="MKVTFPHMGSLSIALEGLLTDLGLEIVIPPPITKKTINLGVLNA
                     PEFACFPLKITLGNFIEGLEAGADTVVMLGGTGPCRLGYYAEVQKEILRDLGYDFEMI
                     VLEPPQGKVTDLTDELKKLCGTTSWAQMMRALHTVWRKVKALDKLHALSLQVRAREVE
                     LHATTKAYQKSIALLREAQTPAAVNQKLRQGLAILKAVPQDRQKSVLRLGLVGEIYML
                     LEPFANLEVEKILGEMGVEVERSLYISEWMKEHLLLSSMRLKGGLAVKRAAEAYLNNT
                     VGGHGWESIGATVRYARRGYDGVIHILPFTCTPEIVAQSILPTVSKEQGIPVLSFSLD
                     EQSGEAGFKTRLEAFLDLLEQRKKTWSRV"
     gene            complement(91292..92254)
                     /locus_tag="VFC74_08480"
     CDS             complement(91292..92254)
                     /locus_tag="VFC74_08480"
                     /inference="COORDINATES: protein motif:HMM:NF021504.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydratase activase-related protein"
                     /protein_id="HZK25410.1"
                     /translation="MSSTVGIPRALLYYHYYPAWEAFFQMLGAEVVLSDETSKAIVDC
                     GVRAAVDEACLPVKIYLGHLANLRDKGVDYIFAPRVISVEPRKYLCPKFLGLPEIARF
                     SVANLPELLTVEINVQQKYRETLKGLKQLAARFDWRPWKGWQALQAAAAAQKEYEAKL
                     QQGLTPQAALAGKAAQPKSGRTRVAVLGHAYNINDPFISLNILNKLTELDVEVVTSEM
                     LPAAAIAEGSEALEKDVFWTFGHLLMGAGLHFLQQQNIAGLVLVAAFGCGPDSLTCEL
                     IERYYKRQRTVPVILLTLDEHTGEAGMVTRLEAFVDMLRWRKAI"
     gene            complement(92305..93789)
                     /locus_tag="VFC74_08485"
     CDS             complement(92305..93789)
                     /locus_tag="VFC74_08485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009847 - spore germination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore germination protein"
                     /protein_id="HZK25411.1"
                     /translation="MARKHPGPPDKVSPQLKENLDFLKDKLGIGINIDVLKKDLKLGG
                     QEGALLYIDGLTNGEVAVLVLQKLSTLTREDLAPNPWHKLMTQKIPHMEIESNDSLPQ
                     LIDEILAGQMLLLLDGETEGILLDTRTYPARSPEEPDTERLTRGARDGFVETMMFNVA
                     LTRRRVRDPGLRVEGFRLGRRSKTDVALMYIQDITNPELVAMVKDRLERVDVDGLPMA
                     EKSVEEFISDSRWSIFPLVRYTERPDVAAQHLFEGHLLLFVDTSPAAMILPVTLFHHL
                     QHPEEYRQNVFTGIYVRWIRFLAVLMSVILAPLYLLFSQQPELLPEFLSFIGPKEAGA
                     IPLFIQFIIVHFGIDLIRIAGIHTPSPLATALSLIAALLIGQIAIDVGLFTPEVLLYG
                     GLVAVGMFATPSFELSQANRLVHLFLLIVTGLFNLPGFLAGIAIVLLKLITNKSFGVP
                     YLWPFFPPNLRGITSTILRQPVPIERTRLTILHPSDQDRMPPKQ"
     gene            complement(93872..94075)
                     /locus_tag="VFC74_08490"
     CDS             complement(93872..94075)
                     /locus_tag="VFC74_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HZK25412.1"
                     /translation="MSVAKIVELVGESSKSFDDAVKNAVAEAARTIDNITGVEVSNLT
                     AHINDGEIVEYKANVKLAFGVKR"
     gene            complement(94214..94846)
                     /locus_tag="VFC74_08495"
     CDS             complement(94214..94846)
                     /locus_tag="VFC74_08495"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK25413.1"
                     /translation="MDDSFNKHLPLVHSLVKRYQGEYAEGDDLFQVGSIGLLKALKNY
                     DKSRGTAFTTYAVPVIAGEIKMYLRGQGSVKYSRAMKQMAGRVKKVREALEQRLGRQP
                     TLSELAKVCDLEREELLAALDVARGPISLDSEETKLAVTPAEAETVVDRVALSQALSS
                     LPERERQIMLYRFFKQKTQQEVAEILGLSQMHVSRLERKVLQQLKQQLNG"
     gene            complement(94854..95291)
                     /gene="spoIIAB"
                     /locus_tag="VFC74_08500"
     CDS             complement(94854..95291)
                     /gene="spoIIAB"
                     /locus_tag="VFC74_08500"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor"
                     /protein_id="HZK25414.1"
                     /translation="MDNYMKLEIPSKSQNEAFARVVVAAFAAQVDPTIDEISDVKTAV
                     SEAVTNAVIHGYENGPGTIVISAILVDSRLEVTIIDYGVGIADLEQAREPLYTSKPEL
                     ERSGMGFTIMENFMDSCHVVTEPGKGTTVTLVKTFGRADVTEA"
     gene            complement(95303..95638)
                     /gene="spoIIAA"
                     /locus_tag="VFC74_08505"
     CDS             complement(95303..95638)
                     /gene="spoIIAA"
                     /locus_tag="VFC74_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045152 - antisigma factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma F factor antagonist"
                     /protein_id="HZK25415.1"
                     /translation="MQVKLKKAGSTLIVQMVGELDHHAVDAMRKKIDLKLNHEDFRNI
                     LFDFYGVNFMDSSGLGMILGRYKIVSEKGGKVSACSLNSRVMRVFEMAGLQTKIPVYS
                     SEQDALTGA"
     gene            complement(95877..97046)
                     /locus_tag="VFC74_08510"
     CDS             complement(95877..97046)
                     /locus_tag="VFC74_08510"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZK25416.1"
                     /translation="MRNKLLLVVLVITLVLASALPALAIEFEFQSSAQLLLDMASGQV
                     LYENNAHEKLYPASVTKVMTMLLAMEALESGKVTLTEMIPISERAAAHGGSQIFLSPG
                     DRVSFEDLMIGIVVGSANDGAWAMAEFLAGSADVFVEQMNARAKELGMKNTHFVNPHG
                     LHDDNHYTTAFDIALMSLELSQYPKIHEWATIWMDEDFLKGKIKKEEGVYLSNSNNLV
                     RYYDGCDGLKTGFTDEAGNCIAATAGREQTRLLAVIMKAAGRPVLFEEARELLNWGFA
                     NYQSVPIVKGGEILATAAVDKGQSTTVDIVAAADLALLLPKGSAADYEQELELPKRLP
                     APLTPKSAVGRLIVKSADGEELGSISLVPKEEVPRAKLTTFLTRYINHWAQFDRK"
     gene            complement(97127..98443)
                     /locus_tag="VFC74_08515"
     CDS             complement(97127..98443)
                     /locus_tag="VFC74_08515"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018589293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HZK25417.1"
                     /translation="MRILEIMEKKRDSLALTTSEISQFIHAYLSGEIPDYQVSALLMA
                     IFIQGMNHEETVALTDALVHSGEVLDFSGLGRLVADKHSTGGVGDKTTLVLLPLAAAA
                     GVTIAKMSGRGLGHTGGTIDKLSCIPGFRTDLPQEEFLAQVRELGVALTGQTPDMTPA
                     DGKLYALRDVTATVDSIPLIASSVMSKKIASGAQSIVLDVKMGSGAFMRDLVNARQLA
                     RTMTAIGTALGRKTVSLITAMEQPLGKAVGNHLEVVEAIETLHGQGPADLTELALTLG
                     AEMLLAGKLAASRQAATAKLTALLADGSALQMMRRFIAAQGGDPAVVDEPARLGTPPY
                     AREMRAVTSGYVTRVDARKVGQTAVLLGAGRQQKDAPIDLLAGIKLHKKIGDYAQQGE
                     LLAEMFASNEEKLAAAAAELIAAYEFAAQKPAEKPLIIERFAAENL"
     gene            complement(98406..99614)
                     /locus_tag="VFC74_08520"
     CDS             complement(98406..99614)
                     /locus_tag="VFC74_08520"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HZK25418.1"
                     /translation="MKRVIMVVLDSVGVGELPDASFYGDAGSNTLAHTAQAVGGLQLP
                     WLQSLGLGCITEIAGVPCSGQPRAAYGKMAERSPGKDTTTGHWEMTGIILSQAFPVFP
                     AGFPPEIMQPFSKAIGRAFLGNVKASGTEIIAELGAQHLATGKPIVYTSADSVFQIAA
                     HEAVVPVEQLYAWCEMARELLSGDYAVGRVIARPFVGEPGNFQRTANRKDYSLAPPQE
                     MLLDKLKAAGEEVVAIGKINDVFAGRGITASVHTANNDDGMSTLLTLLDNYQQGLLFI
                     NLVDFDMVYGHRNNAAGYAQALERVDGQLAAVSDKMTADDLLIILADHGCDPTFHHTD
                     HTREYVPLLVYGAQVRPGPLGVRETFADVGATVACLLGAAPLAAGKDFSQELGLKCNE
                     NLRDYGKETR"
     gene            complement(99634..100521)
                     /gene="xerD"
                     /locus_tag="VFC74_08525"
     CDS             complement(99634..100521)
                     /gene="xerD"
                     /locus_tag="VFC74_08525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020814690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HZK25419.1"
                     /translation="MEKTLRDFINYLAVEKGLAQNTLEAYNRDLKTFCQYLQRFGVTK
                     IEDVTRNNIVGYLRELQKSGRATATLSRNMASIRSFYNFLFQERRVVENPAADLSSPK
                     IEKKLPRVLSTEEVDLLLEQPDQLQVSGLRDKAMLEVIYATGIRVSELMSLNILDINL
                     EAGFLRCTGKGSKERIIPLGSFAIQHLNTYLNKGRPKLVRERSETSLFVNLHGKRLTR
                     QGFWKILKKYAQKAGIAKDITPHTMRHSFATHLLENGADLRSVQEMLGHADISTTQIY
                     TQVTQRKLRDIYNKTHPRA"
     gene            complement(100619..100861)
                     /locus_tag="VFC74_08530"
     CDS             complement(100619..100861)
                     /locus_tag="VFC74_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25420.1"
                     /translation="MIYFRGKKLTSLLWGFLLLLVVLPLLQFYVLQLRHPQAVIFREP
                     RGAALKVSANVSQSVESLPTPTRFLYYLHDFYQNGL"
     gene            complement(100881..101513)
                     /gene="spoIIM"
                     /locus_tag="VFC74_08535"
     CDS             complement(100881..101513)
                     /gene="spoIIM"
                     /locus_tag="VFC74_08535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZK25421.1"
                     /translation="MRQGRQYSISEHLQENKGMYFLAMIIFTIGIAAGALATRLLTIE
                     QIAELKQIFFNFLDYLTLQEPLNQTMILRRSLLQHGIFLLIIWLSGNLFFGILLAMGC
                     IFYRGFSIGFTVGFLVQQSALPGILFALAAVVPQNIVYVPLTLVAGVFAMSSSLTLLR
                     SRLRKQSLPYGSFMMQYSVLVLILGGCLLVGSLIETMITPVFMRAVASII"
     gene            complement(101575..102108)
                     /locus_tag="VFC74_08540"
     CDS             complement(101575..102108)
                     /locus_tag="VFC74_08540"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HZK25422.1"
                     /translation="MSEKTISRKTIYEGRIVKLHLDNVALANGKQATREVVEHSGAAA
                     ILALNKQKEAYFVKQFRKPVEKELWEIPAGKLEPGESPLACAQRELAEEVGLKANDFR
                     LLSAFYTSPGFASEKIYVYLATGLSVQNEAQPDEDEILEVFLLPLAEAKQMISHGEIE
                     DAKTIVAVLLAVQLGLA"
     gene            102243..102368
                     /locus_tag="VFC74_08545"
     CDS             102243..102368
                     /locus_tag="VFC74_08545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25423.1"
                     /translation="MQIMMQRNSFHFFGTIRELRFFLRTLPRDLTLREFIALQIN"
     gene            complement(102365..103438)
                     /locus_tag="VFC74_08550"
     CDS             complement(102365..103438)
                     /locus_tag="VFC74_08550"
                     /inference="COORDINATES: protein motif:HMM:NF024384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3866 family protein"
                     /protein_id="HZK25424.1"
                     /translation="MFSRHAAVVQAVESQSAQYEVLAVKWGATTGQAINYLPLTGAAA
                     PGDQVLINTMAVELQLGSGGYHFVLANLASPEHTSQGAGHIMKLRYTPQQVRVLAVEE
                     EASPQHALMAEAESLVGMPVIAGELHSMLAPFCIALKAKKPALRLAYLMTDGGALPAF
                     FSNTLRILKERELLCGVITAGQAFGGDWEAINIYSGLLAAKHVLAADVTVVCMGPGVA
                     GTATRYGFSGMEVADNLNRAASLKGQPIALPRLSFADRRPRHQGFSHHTLTALTKATL
                     VPVDLPLPMLAAAENKLLEQQIASSGLASKHRIFRYEGLSLASLADDQPLCRTMGRTL
                     TDDPAFFLAAVATAQHVSLLIEP"
     gene            complement(103487..104329)
                     /locus_tag="VFC74_08555"
     CDS             complement(103487..104329)
                     /locus_tag="VFC74_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25425.1"
                     /translation="MATAIYLILGLGGALVIIPVWSEILKLVGHREKNYLDQEIPQSM
                     GAVFPLVFLVAALWAQQLGLLTAAAVWRVLVTAGGLGLVGLVDDVWGDKRVKGFSGHF
                     ERLIIHGELTTGFLKAALGFLVCLAAVYGLPGSVLLIFWRAALVALSANLLNLLDLRP
                     GRALKVFFLLAFLSVWLVPVETGVLLLFPFLLAALAYLPLDLSGQGMLGDAGANILGG
                     VLGLNLVLTAPAIWQLVYFIALVGLHVLTEKISLTQVIAEHPVLSFLDQLGCTKGENK
                     LWKD"
     gene            complement(104329..105009)
                     /locus_tag="VFC74_08560"
     CDS             complement(104329..105009)
                     /locus_tag="VFC74_08560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HZK25426.1"
                     /translation="MQVSAIIPAYNEAQSIGTTILALQKIAEVEEILVIDDGSSDLTA
                     VQALLAGASVRQLPHNLGKGGALQEGVRLAQGKILCFVDADLGESAKEFTYLLQPVLR
                     DEADMTIAIFPPARRRGGLGLVKGLAIYGIRKLCGYHPLSPLCGQRVLRREVWDNLRT
                     PLAGFGVEVGLTVECLRNGYRLQEIPVQMTHRETGRDLSGFRHRGKQFLHLLRTLQQL
                     WSNRDVKA"
     gene            complement(105022..105828)
                     /locus_tag="VFC74_08565"
     CDS             complement(105022..105828)
                     /locus_tag="VFC74_08565"
                     /inference="COORDINATES: protein motif:HMM:NF022824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper transporter"
                     /protein_id="HZK25427.1"
                     /translation="MFRLRDQIISLVAVFLALGIGIIIGTGLTEDMLVTQQRLLIDQL
                     TSDFQLMREERSQLQANVQALTQDLSLWDQYHQVLYPNIVAGVLSDKNIAVVAHAASI
                     PPNLLTLLQDAQAMLCSVIRIEDRTVLGSDTKNVGQALGTLTMAGQCSQVMEEQLADF
                     LAKEAIKIEIQPTAKPDTLLLLVGERQDLDRSLLAEMTEVCQQAGVTLVGLEWSNVKN
                     SALTELKQQGVSTIDNVETVFGQYSLLSVLRGSSGNFGIKQAAEEFIAIY"
     gene            complement(105842..106987)
                     /gene="steA"
                     /locus_tag="VFC74_08570"
     CDS             complement(105842..106987)
                     /gene="steA"
                     /locus_tag="VFC74_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007287338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cytokinetic ring protein SteA"
                     /protein_id="HZK25428.1"
                     /translation="MRMKWHFRAPVSLRGPIKAGKRTKILVSRLEPGDIALIDHLDLD
                     ELAAQSLVAHKVCAVVNVNDSISGHYPNKGPQVLCEARIPHYDLAGADLMERIHDGEL
                     LTVDHHGIWREQELLATVRKVDQEVIRQQTAAAEANLGNLLDAFVQNTLDYARQEKKL
                     ILDGLTIPPLRTTFAGKHTLVVVRGQSYREDLEAIKHYIAEVKPTLVGVDGGADALCE
                     YGYTPDLVVGDMDSVSDEALIKAKERVVHAYPDGRAPGMARIEHLGLRAAVIKAPGTS
                     EDLALLLAYELGAKLIAAIGTHTNMIDFLEKGRKGMASTFLARLKIGDRLVDARGVSQ
                     LYNGRVSGRSLVLLTLAAGIPVLALVITNPTVRHLFRLIALRVRLAL"
     gene            complement(107137..107910)
                     /gene="spo0A"
                     /locus_tag="VFC74_08575"
     CDS             complement(107137..107910)
                     /gene="spo0A"
                     /locus_tag="VFC74_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA];
                     GO_process: GO:0042173 - regulation of sporulation
                     resulting in formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation transcription factor Spo0A"
                     /protein_id="HZK25429.1"
                     /translation="MLRVFVADDNREFSDLLVEYLEQQPDIDIAGIAYNGRETLELVT
                     EDPPDVLLLDIIMPHLDGLGVLEKINHLPDKPKVIMLTAFGHEDITRKAVELGAAYYI
                     LKPFNMEVLVDRIRLLGNGQSTSSAVSSLTRTVKRTSQSLEADITNIIHEIGIPAHIK
                     GYQYLREAIMMVVEEVDLLSSVTKVLYPRIAEKFDTTSSRVERAIRHAIEVAWSRNNI
                     DTIKKFFGYTINTERGKPTNSEFIALVADRLRLSSRSLH"
     gene            107972..108355
                     /locus_tag="VFC74_08580"
     CDS             107972..108355
                     /locus_tag="VFC74_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25430.1"
                     /translation="MDRNTFLIITIIFKTFLGIKKLCRRGELRSPAWLISVRALDIGA
                     GAQSDAPTVMCKKWRPYLKKTTPFVKVGANCVRPRCLISVRALDIGTGAQSDAPTVMC
                     KKWRPYGNDAQSNTLPKKANTSLGK"
     gene            complement(108510..109829)
                     /gene="spoIVB"
                     /locus_tag="VFC74_08585"
     CDS             complement(108510..109829)
                     /gene="spoIVB"
                     /locus_tag="VFC74_08585"
                     /EC_number="3.4.21.116"
                     /inference="COORDINATES: protein motif:HMM:TIGR02860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIVB peptidase"
                     /protein_id="HZK25431.1"
                     /translation="MKRRWKRQAPAWLLLGIVLLLATAFPWRIWVAMQNELRILEGCQ
                     YYVNIASPLSIHVKGDKAGILSLNGSPVSVQSCKLDLSSPLQFSAQSLGSVNLEFKLF
                     GVIPLRQLTVNVLPEKKFIPGGHSVGIKLHSEGVLVVGHHLVQSQGKSTSPAKEAGIE
                     VGDVLLTIEGTKLEDANQVAEIINQHKDKNTLKFEIKRGERSFTAKVNPSYCQETQRP
                     RVGLYVRDSAAGVGTLTFYDPQTKIYGALGHVITDVDTNQPIEVKNGQIVKANIVNIK
                     AAQRGYPGEKTGIFQEDQDLAGSIEKNCQFGIFGRLSSIAPMPGSENKALPMALASQV
                     KPGAAQILTVLEGTTIERFDIQIQRVYNQSQPSDKGIIIEITDERLLDATGGIVQGMS
                     GSPIIQDGRLVGAVTHVFLNDPTRGYGIFLEWMVAESSLLAANNNNI"
     gene            complement(110144..111781)
                     /gene="recN"
                     /locus_tag="VFC74_08590"
     CDS             complement(110144..111781)
                     /gene="recN"
                     /locus_tag="VFC74_08590"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HZK25432.1"
                     /translation="MLRVLEVRNLALIDNLAFYPGFGLNVITGETGAGKSMLLNAVSL
                     LLGERASADAIRTGEESALIQAIFTWEESKDATVKEEELIFSREVRRSGPNVCRLNGR
                     VEPLAQMAVYGRQLVDLHGQNTQQSLLEVSTQRALLDAYGGEKLTQCRTQVSAQYKNL
                     QQLQKDLQELGGDDAAVLRQADFLRFQLAEIEEANLTSAEEEELAVRWRRLSNAQQLI
                     ERTAGVYAVLCEGSFDATIVDQLGLVEKELAAAADLDATLQEILQQISSAAEQIREAA
                     RELRYYQEELHLDEEELLAVTERLETYRHLKQKYGPSLAQVADLTAEMQQELAVLTGR
                     RAKREELTAMIAETKAALQKAAAELTRLRQETAVNLGEQINLALQDLALQGAQFVIVV
                     TPEDQYSAYGCDKIEFQLAANAGEPLRSLAKTASGGEISRVMLAIKSVLAAEDAVATL
                     IFDEIDAGIGGLTVRAVAEKLKQLAAHRQVICVTHQPLIAAAADHHFLIYKESSNNRT
                     ATKLKKLSAAEREVELARMLGGEDEAALAHAHQLLAN"
     gene            complement(111789..112667)
                     /locus_tag="VFC74_08595"
     CDS             complement(111789..112667)
                     /locus_tag="VFC74_08595"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HZK25433.1"
                     /translation="MKVLGIVPNWKKLANVKIMVERIADFCRAHGMQAFLVASDELEP
                     LADLPVLDTADFPGKVDLVLVLGGDGTMLRAAHQFSGTNLPLLGLNLGQMGFLAEVEP
                     PMLEEALQQLLLGNYRINHRIMLWARVFRGDRQLVEYTALNDVVISKGVASRIIYLDT
                     YVNDKHLETYPSDGIIISTPTGSTGYSLSAGGPIVNPELDVMIITPICPHLLHHRSVI
                     VDSKERVVVRPWTRDIEVTVTIDGQTGITLQDDDIVQVTRAPLTTPLIQVQGTDFYTL
                     LHQKLTKVISWQPERM"
     gene            complement(112681..113094)
                     /locus_tag="VFC74_08600"
     CDS             complement(112681..113094)
                     /locus_tag="VFC74_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25434.1"
                     /translation="MASKDWVLLGLVILVIVARWLAGRSGRIGGKKTTQARGKAVQIL
                     EEAGCEILVVKPTLTINMEIDGQLHPFTLKSDYLVKRHGQRYLVRIRRDNKQVRLQSK
                     LWRSSLLRDVLAFRARGILILHLEKETLQEVRFRT"
     gene            complement(113119..113952)
                     /locus_tag="VFC74_08605"
     CDS             complement(113119..113952)
                     /locus_tag="VFC74_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016323481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HZK25435.1"
                     /translation="MGKERKRLDTLLAERALFVSRSAARAAVMAGLVSVDGQQVDKPG
                     TQVTANVAVTVKELPRYVSRGGLKLEKALAAFQIELAGRTAMDVGASTGGFTDCMLQQ
                     GAEKVYSVDVGYGQLDWRLRQDSRVVNLERTNIRYLEAEALAEAVQFASIDVAFISLA
                     LVLPTLQRLQVPEIVALVKPQFEVGKGRVGKKGVVRDPADHLAVLQKVASLAVELNYD
                     VAGVSYSPIRGPEGNIEYLLHLLAATDAAAIAKEIDFAATVAEAHQELTEKRKPANRD
                     E"
     gene            complement(113928..115841)
                     /gene="dxs"
                     /locus_tag="VFC74_08610"
     CDS             complement(113928..115841)
                     /gene="dxs"
                     /locus_tag="VFC74_08610"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HZK25436.1"
                     /translation="MSKLLPTITGPADLKKLSNKELTALAGEIRAFLLANVSKTGGHL
                     APNLGVVELTLAIHTTFDSPTDEIIWDVGHQSYVHKILTGRADRFASLRQYGGMSGFP
                     KIKESEHDIFQTGHSSTSISAALGVAQARDLLGKDNHVLAVIGDGAMGGGMAFEALNH
                     AGDMGTNLIVVLNDNEMSISHNVGGLAAYLGRIRTDPKYFRFKEDFQELLLKIPAIGG
                     KMIKSVDRIKDAVKSLIVPGMLFEELGFTYLGPVNGHNLEAMQGVMRGAKKLKGPILI
                     HVLTKKGKGYDFTENNPDFYHGVGPFDPQSPPQPGTRGSVPSYTDVFGDTMVQLGREQ
                     QKLIAITAAMTSGTGLSGFAQTFPQRFFDVGIAEQHAVTFAAGLARKGFKPVVAIYST
                     FLQRAYDQVLHDVCMQELPVVFAVDRAGLVGEDGETHHGVFDISLLRPLPNLSIICPR
                     DENYLQHALFTGLKHNSPVAIRYPRGKGQGAALETPRCLPWGRGEMLRQGRDVTILAV
                     GTMVAPALAAAAQLEKNGLSVAVIDLIFVKPLDTELVVRAALNSRYGLVTVEENVLAG
                     GLGSAVLEYLEDNGLQTLPVKRLGIPDQFVQHGARNLLLKDVGLTVENIAAACLGLAS
                     SKGKISWVKSVND"
     gene            complement(115852..>116274)
                     /locus_tag="VFC74_08615"
     CDS             complement(115852..>116274)
                     /locus_tag="VFC74_08615"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM domain-containing protein"
                     /protein_id="HZK25437.1"
                     /translation="TGILFDMIGIAATAADEVPILAKAAKKTPGAREALYLIRNADRF
                     SNFCNDVVGDIAGIISGVLSALLVVRIFGAESNPVVSIAATGIISALTVGGKALGKSV
                     AIEHSTVIILLFANVLTKIETVLPGKIFFGRALPRKRK"
BASE COUNT        31407 a        29656 c        24485 g        30726 t
ORIGIN      
        1 gtgccaatac tactgacatc gccttgctgg agcttgggta gggtaaacgc cataatctgg
       61 ttaaagatgt caccggcagc aattaattct gataaaaaat attcaccggc ttcaaacagt
      121 ctgccaacct cttccatcgc ctctttgcag gtctccaaaa ccgccactgg gccgccatcg
      181 gtctccacca attctttagc aatccttact gcttctcgct cgtccatatc gacaaaactc
      241 tttagcaact cttcccgcat taaaaactcc acctccgatt ttttgtctac agcctaaaag
      301 ggatactccc ttcagctagc ttaatgacta cccttacttc ctaatgtagt ctaatgtaat
      361 gaatctagta cttaccgtat ttctccacag cttcgtaaac ggctctgata ttttcaatgg
      421 gggtttcacg tggcaaagtg ccaatattcg gcgaaaggat aaaaccacca cctggcatca
      481 tttcacctaa aagctcctta atgtattcat ctagtttggc aggagtactg gtaataatca
      541 tgcccgtatc aataccaccc ataatgcagg tatgaccttg gagcaacttt ttcgctttgc
      601 gcacatctgt cttttcaaag tagccaatac cccagccagc aggtaagtct aaaattgtct
      661 ccagatgagc atcatgatga ccctcaaaga aaacttgtga tttcatgcct tcctgcagca
      721 tacctaaaat accttcctta agggtaggcc aataaaactc atggtagagc tttggtgata
      781 gatactcatt taaatgcaac ggaataaagg aaatatctat acctcttgct ttaccactta
      841 aaaccatctt cagtagtggc tctaaaagtg cttcagctgc tgctttgact ttgtcaggat
      901 agcgacgtag atccttaatt atattgggaa tatcgcgtag ttggtcacca ataaagtcta
      961 aaggagctat aaatactgct ctccccgaag gagcatagcc taactcttgc agttccttta
     1021 tcatattttg catagcagct ccctgtttgg atttctccaa acccagcctg gccatcgcag
     1081 ctaaagcagt aggtgacccc aagttttgca gattagtggt agtgcggggc accacttttt
     1141 caaacataaa ttttgccgga tctgcaatta actcatcgta ctcttccggt tccatataag
     1201 cgctgccaat aaactgaggg ctgctgttct catctagttc ccgccctggg aaacggcagt
     1261 attttacgcc caatatatca ttagtaggac catctacact ggagaccagc ggagctagat
     1321 caggataatc aaaaaatgct aaagatgtta cgctattacc taaactcggc atcccgagca
     1381 tcggcagatc actgggaaag tcttttaaat atttgagtgc ggcttgattg gccagttcat
     1441 agtcaaagca atattgatag ccggtaatgc cagcataagc agctactaca tcgccaccaa
     1501 gacccgtgaa aggcactcga tcaggctctt ttaattccac agccgcttta tatcttgctg
     1561 cacgttctgc agccaatttt tttgcatcaa acgtcatcct catcacccct tatttcaata
     1621 atctttttct aaacgcgtac cgcgagtgta tcatcagcct tggcacatta ttgactgcta
     1681 tcaaaagtaa gtagatctgc ttcagtgaat cacatacctc taggcccacg gtgggttgat
     1741 ggtatgctga caggggctta ctcccctgtc agcatattta tctagtttag tacttcccgt
     1801 atttctccac agcttcgtaa acggctctga tattttccat gggggtttca cgtggtaaag
     1861 tgccgatatt aggagaaaga atgaaaccgc cgcctggcat catttcacct agcagctcct
     1921 taatgtattc atccagcttg gcaggagtac tgccgatcac catccctaca tcaataccac
     1981 ccataataca ggtgtgatcc ttgagcagtt ttttcgcttt tcggacatca gtcttttcga
     2041 agtagccgat accccaacca gctggtaatt ctaagatcga ttccatatgc gcatcgtggc
     2101 gaccttcgaa gaatacctgc gatttcatgc cttccttcag cattcctagg atacctgcct
     2161 taagagtggg ccagtaaaac ttttggtaaa acgttggtga aagatactca tttaaatgta
     2221 gcggaataaa agcgatctcc gcgccgatat ttttgccaaa caaagccagc tttaacatgg
     2281 gctcaagcaa tgcttcagct gctgctttga cttggtcagg ataacgacgt aaatctttaa
     2341 ctagattaac agtgtcacgt aggttatcac tgataaaatc taagggggct aagaagaagg
     2401 aaccagcact gagtggataa cctaaggctt gcagttcttg gacaaatcct tgcgaggcag
     2461 ctgccgcctt agctttctgc atgccaaatc tggccatggc agccaacgca gtaggcgaac
     2521 ccaagtctct taagttcgtg gtagtgcggg gaacaatctt ttcatataaa aacttggttg
     2581 gattagcgat caactcatca tattcagccg gttccatata ggcttggccg ataaactgtg
     2641 ggctgctgtt ttcatctgtt tgccgccctg ggaaacgaat gaagtttact cctaagatat
     2701 cgttagtagg gccatcaatc ataccaattt gccccgctag atcaggaaaa tctaaaaatg
     2761 ccaactgagc aacggcatga cctattcctc caccgcccat gataggacga tcactgggga
     2821 actctttcag atacttaagg actgcctgct gagccttttc cggttcaaag caaaactcat
     2881 agctggtaat gccagcatag gctgccacca catcgccacc catacctgta aaaggcaccc
     2941 gatcaggttc ttttaactcc accgccgctt gatatcttgc cgcacgttct gcagccagtt
     3001 tttttgcatc catcgtcaaa ctcttcaccc cttatctaat aatcttctat ttgaatacgc
     3061 ttttaaataa taatcattcg cgggaatatg ttcaccccta gcactttaca ctgtaatcaa
     3121 tttatatatc ttgcttgctg acagaagaat aatcccctgt cagcaagctt aaataagcta
     3181 atatttaccg tacctttcta cagcttcata aacggctctg atgttttcaa tgggagtttc
     3241 acgaggcaag ttgccgatgt taggggagag aataaacccg ccgcctggca tcatttcgcc
     3301 taatatttcc ttgatgtatt cgtctagttt cgccggagta cccgcgatta ccatgccgac
     3361 atcaatacca cccataatac aggtatgtcc ttgcagcatt tttttcgctt tacgcacatc
     3421 tgttttctcc atgtaagcag taccccaacc aacgggtaga tctaatattg tctccatatg
     3481 cgggtcgtga cgaccttcaa agaagacctg tgatttcata ccgagttcag ccaaccgtaa
     3541 aataccttcc cttaaagtgg gccaataaaa ttcattatag agggttggcg ataggtattc
     3601 atttaaatgg agaggaatca tcgctgtgtc aacaccagga ccctgggcat tggctgagac
     3661 ctttagtagg ggctccaaca ctgcctcggc agctgcttta gccttgtcag gatatctccg
     3721 caaatcttta accaaattgg gaatgtcacg caactgatcg ccaataaaat ctaacggcgc
     3781 aaaaaagatg gattgaccac ccggaccgta gcccaattct tgcaattgca caaacaaatc
     3841 tcgcatagca gcggaagctt tggctttctc taaagcgagt cgagtcatgg ctgcatgggc
     3901 agtcggtgat ttcaaatctt taaaagcagt taccatacgg gggagaactg tttcatagca
     3961 gaatttcaca ggatcagcaa ttaatgcatc atactcctcc gcctccatgt aggtagagcc
     4021 gataaattgt ggactgctgt tttcaccaag ttcccgtccc ggaaaacgac agtaattaac
     4081 tcccaagata tcattagtcg ggccgtctac acgggagata atggggaaaa tatcaggata
     4141 ctccaaaaag gccagtgaag cgacatggtt acctaacccc gtcataccca tcgctggccg
     4201 atcactggga aaatctttaa catacttgag aaccgcctga gactgctttt cgtaatcaaa
     4261 gttaaactca tagctggtaa ggccggcata ggaagctacg acatcgccac ccatgcctgt
     4321 aaaaggaaca cgatcaggtt cctttagtgc gatagcatcc ttcatccttg ctgcacgttc
     4381 ggcagctagt tttttagcat ctattgtcat tctcttcccc ccttacgcta gtaattcttt
     4441 acttaagcga acaccacgag cggcgtcatc ggcccaagca tcagcaccca cgtgttccat
     4501 cacagcttcg ctgacccggc cgccaccgat tataatctta atgccatcac gcaaacctgc
     4561 ttctttaatc acttctactg tctgcttcat ggaatccaca ccgagggtaa ttaagcagca
     4621 catgccaaca atgtcgggct gatgctcctt gatagcttcc acgaactttt catgcggtac
     4681 atcaatccca atatcaatta catgaatacc agaggcttct gccattactt taaagatgtc
     4741 tttaccaatg ttatgtacgt caccctgaac agtacctaaa accatggtac caatactgct
     4801 gacatcgccc tgttggagct tcggtagggt aaacgccata atctggttaa aaatgtctcc
     4861 agcagcgatt aactctgata agaaatactc gccagcttca aacagtctgc caacctcttc
     4921 catggcttct ttacaagtct caagcaccgc tactggaccg ccatcggtct ctaccaatgc
     4981 tttagcaatg ctcactgctt caacttcgtt catgtcaaca aagttcttta atagttcttt
     5041 ccgcataaac tagtccacct ccaaaataat tttaaatgct catacctact tgcctactta
     5101 gatctgacag agggaatacc cctgctagct attaatctag tactttccgt acttctcaac
     5161 agcttcgtag acagctctaa cattttcgat gggagtttcg cgtggtaaat agccaatatt
     5221 tggtgagaag ataaaaccgc caccgggcat catctcagca aatagttcct taacatattg
     5281 gtcaagcttc gctggtgtgc tgctaatgat atcactaaca tcaataccac ccatgatgca
     5341 ggtatgcccc tgaagcagtt tctttgcttt acggacatcc gttttttcaa agtaggcaac
     5401 accccaacca gcaggaagtt ctaaaatggt ctccaagtgg ggatcctgcc agccttcgaa
     5461 gaaaacctgc gatttcaatc ctgcctcaaa cagcttgagg ataatctctt tcaaggtcgg
     5521 ccagaaaaat tcgttgtaga gtttagggga gagatattca tttaagtgca gcggaataaa
     5581 tatatacttt gcccccacat ctttatacat caaggacatt ttcaacaatg gctccatcaa
     5641 agcttcaacc gcagctttaa ccttatccgg atgccgccgt aaatctagaa ctaagttagg
     5701 aatatctcgc agttggtcac ctatgtaatc tagaggagcc caacctaagc ctctaacaac
     5761 agatggataa cctaacgccg ctagctcctt acctaaagct cgactagaag ctctatgctt
     5821 ggccatctcg atacctaatc ttgccatagt agctaaagcg gtaggtgaag acaaattttc
     5881 taaatttgtg gaagtgcgtg gcactatctt ctcataagaa aacttaaccg ggtcggcgat
     5941 caattcatcg tactctttcg gttccatata agcaccacca aggtattgca tactgctgtt
     6001 atcaggtagc tctctgcccg gaaaacgaca gaattttact cctaaaacat cattatacgg
     6061 acccgtagta aaagcaatgc tggtagaaag atcgggaaaa tcaaggaaag ctagtgacag
     6121 aaagatgttg ccaatgcctg caccacctga agcaggcaaa tcactgggga agtctcttaa
     6181 atacttcaca gtagcctggc gttgcaagtc atagtcaaaa gtatactctg cattggaaat
     6241 gccaccataa gcggccacga tatcgccacc caatccggta aaaggaaccc gatctgtcgc
     6301 ttttaactcc atcgccccaa agaaccttgc ttcacgctcc tcggccaggc tttgtgggtc
     6361 cttaactgtc aagtttctca cctctattcc gataattcgc ttaagcgagc aaacgagcgg
     6421 catcagcttt cgctgacagg tcgcctctaa atagttaaaa atccaatata ctgtctacta
     6481 agagctgaca ggggggcgtc cccctgtcag cttattaaac taatacttac cgtacttctc
     6541 tacagcttcg taaattgccc gcacgttttc gataggtgta tcacgtgggc aggtgccgat
     6601 gttgggcgag aagataaatc cgccaccggg cgccatttca tcaaagagtt tcttaatttg
     6661 ttcgtcaagc tgagccggtg tgagggctat gatgtcacta acatcaatac cacccataat
     6721 gcaggtatgc cctttaagca gctttttagc tttgacgaca tctgtctttt caaaataagc
     6781 agtaccccag ccggcgggaa gttcaagaat tgtctctaaa tgggcatcat gccaaccttc
     6841 aaagaaaact tgagctttca tgcctgctgc gaccagcttg agaataactt ccttcaaggt
     6901 tggccagtaa aactcgttgt attgtttggg cgagagatat tcattcagat gcagcggaat
     6961 aaagcattcc tttgctcccg cttttagagc catcgtgccc attttaaaca tcggctcgac
     7021 caatgcttcg gcagcggcct taactttatc gggatagcgg cgcaaatcca gaactgcgtt
     7081 gggaatatca cgcaactggt cgctgatcaa atccagagga gccaagcaga cacctctggg
     7141 agaaaacgaa tagcctaatt ccatcatggc ttggaccgtt ttcgccattt cagcatcctg
     7201 tctggctttt tctatgccaa aacgagccat ggttgccaga gaagcaggag aacttaaatc
     7261 ttttaaacta ccggaaatac gcggcatgat gacttcgaaa cagaatttct gcggatcggc
     7321 aatcaactga tcatactctg atggatccat attgctgcca ccaatgaact gccgagcact
     7381 gtattcatca agctcccggc ccgggaaacg cacgaacttc attttgagaa tgtcattcac
     7441 agggccgtta atcgcaccaa cgccacctgc gatatcggga taatcttgaa aagctacaga
     7501 cattgactgg ttatctaacc ctccgacacc aaccctggga gcactatcac tggtaaattc
     7561 agaaaaccat ttaattgctg cctgacgatt cttttcgaaa tcaaactcat attcatagga
     7621 agaaagccca gcataagcag gaattacatc gccgcctaaa cctgtaaacg gcacacgatc
     7681 aggctctttt aaatctgtag ctgcctgcat tctggccgca cgttcttgtg ctaacttttt
     7741 catttcagga ctaaccattt agttcactcc ttatcctagt agttctttac ttaagcgaac
     7801 accacgcgcg gcgtcatcgg cccaggcatc agcgcccaca tgttccatca cagcttcact
     7861 gacacggccg ccaccgataa taatcttaat accgtcgcgc aggccagctt ctttaatcac
     7921 ttctacagtc tgcttcatgg aatccacacc caaggtaatt aagcagcaca tgccaacgat
     7981 gtcgggctga tgctccttga tagcttccac aaacttttca tgcggtacat caatgccaat
     8041 gtcaattaca ttgatgccag aggcttctgc cattacttta aagatgtcct tgccaatgtt
     8101 gtgcacatca ccctggacag tacctaaaac catcgttcca atactgctga catcgccttg
     8161 ctggagccta ggtagagtaa actccatgat ctggttaaaa atgtcaccgg cagcaattaa
     8221 ttctgacaag aaatactcgc cagcttcaaa cagcctgcca atctcctcca tagcctcttt
     8281 gcaggtttct aaaaccttaa ccgcaccacc atcaccctca accaattctc tggcaattct
     8341 tactgcttcg acctcatcga tatcgacaaa actttttaat aactgcgttt gcattatcac
     8401 tttcacctcc agtttatttt gcaatagtgt acaatctcag acataataca cttttatgct
     8461 caaaatagtt acgatatacc cgtttgtctt actgacaaca actaatttga ctggagtaag
     8521 tgtttatttg attaatgttg gaaaattcgc aacttgtcca cgacttaaac ataccataat
     8581 ctgcatttgc ttgcaagagt tttctgaatc ttttatattg ggcaataaag cagattaatc
     8641 tgccatgatg cgaaactggt tttagctaaa ggaaaagagg ataggcctac tagcaacggc
     8701 ttatcctctt tgtttatcat gtatatttag ttatatttgc tgttccttat gcaaaagctg
     8761 ctctttaaaa ctctccgagc ctgataatct gattggcaat atcgattgct tgacggctgt
     8821 tcatggttgt gtcgccatca agagcaacga caattacata atgatcaaaa taacacgcat
     8881 accaccccaa ctgcctctgt ccggaaattt cagccgtacc tgttttaccc tttatgtcca
     8941 aaccttcgag attgccggca ccagccacag cacctggttc ctgaatagct gccctaaggg
     9001 tctcgtctac taatctaata ttctccggtg catagccggt atcaagccat gtttgcggca
     9061 attctcctaa aaaaaactgc ggttttggga tagtgccatc tcctctggga aaaagtgaaa
     9121 acattaaggc taaatgcaga ggcgacatct gtagttctcc ctggccataa ccactatcgg
     9181 ccagtaaaac atcagagagc ggctttgtat ttattttgga gctgtgtaca gttaaaggaa
     9241 aaggaatcgt ctcttcaaag ccaagcagct tggcatgtct ggtaaattta tctgcgccca
     9301 gcttcacgct taaagcagca aagtagacat tatccgagta caccatagct ctacgcagat
     9361 taacggctcc ggcaggtctg ccgacccggg tcacataatg gttaccccag cctgaacttg
     9421 cctgccattt ttgcggtgta tcccagcttt cagcagggtc aaagatattt tctgccaaag
     9481 ccattaaggc cgtaaatggt ttaaaggttg atccgggtgg catttgaccc gtcaaggccc
     9541 gattggcaaa gggactgttt ttttcttgga gtctgctgta ttgggcctga gtaataccca
     9601 aagcaaataa gttactgtca taacccggat tactgcccat ggttagaact tcaccgctga
     9661 tgctgtccaa gataataact gaaccggttt gttcagccaa taccttatcc gttacaaact
     9721 gcaaatcact gtctaatgta gttataagat cctcaccgtc aactactgct gtttcagcca
     9781 ccgttgccgc cgcgttatta gctgcatcat atattgtaat tgcaaagcca attgtaccgg
     9841 caagagtcgt ctcgtatgct gcttcgagac ctgtaaaacc gatataatca cctgctcgat
     9901 aacccagggg tcgtaaatcc tctaatttct ctttggttat ctctgaaaga tagccaaccg
     9961 tttgccccag tgttggcgga acatcataaa ctctgccttg aacctgttta atcatgacac
    10021 cctgcagagc taaaaactgt tcactctttt cctgccagta agcctcagaa atttttttga
    10081 tgggtacata catctcaggc atcacccaag actgagtgta agctttttct attgcctccg
    10141 ggctcaattc taaaagagac gccaaactat ttaagaggaa attctcgtca ccaattttgc
    10201 ctggcactac gcctatttca cggactgtgc ctgaataagc taaaggttta ttgttgcgat
    10261 ctaaaattga gcctctttgg ggaaagtgac gtgttaccct cgcagcgtag gctgcctcta
    10321 aatccggaaa tacgagatta tgatcccaag ctaaaaacca gctttcttcg cccttatgta
    10381 atgtagcctg gtaatgatga gagaaacgtt caactgtact tgtttcaaac tccagggtat
    10441 agctaactgt aacctgctca tcaacaattt caccaacctc gacctcgtga atggtaattg
    10501 ctttaagccc aataccgctg gaaatattat tatatctgct gataaatgta ttttcatcat
    10561 gaattgtttt tgcctcatcc gttaataact gatacattga ctcgtattct tcgctattcc
    10621 agtgctggat gaataactca atgatttcat ccggttcggg gaagctgctg ccggcacaac
    10681 ccacagcaat cagcagtatc attaaaatgt acatgaggaa aaacagtttc tttttcattt
    10741 accatcaact cctaaagtaa ttcccttcat tatataagta acaatttcta cagcatactg
    10801 aaatctcctt caaaacacac aggtacgtca ttcaagaacc ctagagaagt ttcacggcaa
    10861 ataaacagcc caactgccag ttaaagactc gcggttgggc tgttgagtat ttttttcagc
    10921 taaaggcagc ctttcccccg tgcttggagc cacaggacac aagggtttcc taagctgtgc
    10981 ttactccaat actacggtgg aaacgcaagc tgactaaact cccgcttgtg aagctgagtc
    11041 agcttctctg ttagcacgaa gttgattttg catgtttagt acagtctcaa tagtaagctt
    11101 gttaaagaga gttgttatca caagggcaac ggcaataaaa agcatcggta ctaaatgaat
    11161 aatagcacga atattggcag ccaattgcgg agtatactcc atctccgcta catagccgat
    11221 tgctgcaaga gagaagccaa tcacaccact ggaaacaatt ttgcctagtt tgggagaaag
    11281 gttagttaga ttcatgataa atggcgtggc aactttgcct gtcttaagtt ctccatagtc
    11341 gcagcagtcc gagaacatgg caaatgaaat ccccatcata aaggaaacgc cgacctgatt
    11401 gatgcccata aaaataataa atgtgattaa atctcgggca aagagataag ccagtgtgtg
    11461 tccaactaaa caaaggacta aacctgccgt atagatatgc cttttatcca tgtacttact
    11521 gacaagttga accagcagag atgctacgac acctatcacg gctatggagg acatgaaaac
    11581 aggcattaag gacatgtttt gtataatata acggaagtaa taggcaatag tgtaggttaa
    11641 ggcaaaagca gcgacattgc gggcagtttc gccaaaaatc aaagccagca gcgggggact
    11701 cttaaaaagc tgctgcagca tttctttgcc tgatatatta cttttctttt gctgtgccgc
    11761 actttgggag ttattacggg tttcataggg ttccgtgact ttaaatacaa gccaaaacat
    11821 agttaacatg acagaggaaa gaactagtga agtaaaggta tagccgcgaa ctgccggcac
    11881 ggtcgcacct ctggcagtaa aaaagagcag catcggcatg gagattgcgc caaaaataaa
    11941 gcggctaatg gagctgaact gtaatcttct gatggaaagg atggctctat cttcaggagt
    12001 tttgcccaaa ataggcagga ttgcactaag cgcaccaaaa gagagattgt atgagccatg
    12061 ggcgatagta taaataatga tgatccaaag gtaacgaaat actccttcta aaggcggatt
    12121 agtaaaaatt agcgtaaatg tgattaaggt gatgggtggc ccaatcagca tccaagaacg
    12181 gtttttgccc cacggtaggt ttgatttttg cataattaca ccggcggttg gcacagttaa
    12241 catatcaaat aagttcaaaa ccatcatcag cgtagccatc acagcggcag gcatcaatag
    12301 tacatcggtt agaaaaaagg catagtagtt cgaggtcata ctgaccatga gcgagaaacc
    12361 taaattagca agtccatagg tattaacaac tacatttgta actctttgcg gtgctgacat
    12421 cttattctcc tccttgtttg agcatgaacc acaacttcct gacgatcgtt ccttaaggca
    12481 acagcactca ttattggaaa aaaactatta ctaaggtata ggaagtactt atattagcgc
    12541 gaatgttaga taattcgata ttatcacacc atttaggcga tgtaaactgt gtttccaggc
    12601 ttcttttggc ctaacagcat cacagcactg atgtaaagga tacaatattt ctgctctgtt
    12661 aattctagat aaatgtaaaa atgagcagac acatcatgcc tgctcatttt tagggaaatc
    12721 tctcgtactt ttggtaaaag gtacggccat aattatctgc ttcctcgttt aaccatttta
    12781 ctcgttcggg atttctttcg gtagcgccac tgcaccagaa aacatacctg cctgttggtg
    12841 cataggaatc taaagccagc cgcaccgatg ctcgtaccgc ttcttcatct gaatcagtct
    12901 gattccccgg gccaaaagcg tcccagccac cgatacagac aatgccgtaa tcccgcttgt
    12961 ccttttcaat atcattaaag acttggaaag gttgaatcac tgaaacaccc atgtctacag
    13021 aatcagcaat taaaggatca acataaccat caacatgggc ggcgtttact gttaataagc
    13081 gctgaaaatc tttactatct aaacttagcg ggaagtttaa gaagcaccaa ttcccgcaaa
    13141 acctaactgt gcaagtacag ctttcggaaa ctcaaagcga atagtgagct ccgggtccac
    13201 aacttgacag aacttcaagt tgcccacaag gctcatcaaa cggccggcat ccccttggct
    13261 caaaccgacg atacccgtca agaaatcgaa catgttatcc acggcctctt tgagcgcttt
    13321 gtccgaggtt tttgctgtgg caatggtcgc atatagctcg gcggtctcaa caaaaggtgt
    13381 cggcagctgc ggctcatcca ctacagcggc cgcaagggtg atctgtgccg gtgtttcggc
    13441 tccacagacc aaaacctcac catcgcccat caaggcgtgg gcatcaccgc agccaaacag
    13501 tccgtcatcg acagcagcgt gcaggtaaag cgaggcgccc tcaccgatta agttgcaatc
    13561 catattgccg ccgtgtttgc ccggtgtgcc attggggatg cttccttcct gcggggccaa
    13621 gccgattaca ccaatcattg gttttaacgg caaatgcaag tcccccttct ccgtcgagac
    13681 ggagagcacg ccattatcat tgtccatcac ccgcgttgaa gcctcagtga taccgctaac
    13741 cattccggcg ccgggaatag tggtcatgac tgatttacca gtcatctcca gcttcttgat
    13801 gtcgatacga accagatcgc caggtttaac accttcgatt gcgactggcc cggttgcagg
    13861 attgattcga tcccaatcca atttatctag ggtgtctgca tcggagctca attggttggc
    13921 aaagcaatca caggtattaa gtataaactc ctcatgttgg gcgaccatca aaaggggctt
    13981 cagggtcggt gcaaaggcgt agaatacctg atcatgattt agtaatttca tgcctcttcc
    14041 tcctgtttct tattttttat cattttatct ctctttttcg aaccgctggg taatctgcgt
    14101 ttgatgcttt gccaggttag ctctctccca ccggtgatac cgtccgcctg ccagaccaaa
    14161 aatatgatca tcataatagc aacgacgatt tctgtcgaac cagatgcttc aaagccgctc
    14221 ctggtgagaa acagctcagc ttgccgtaag ccctgccgta acagagtaac taagagcgtc
    14281 ccgaaaaagg ctcccgagac agacattgag ccgcctatta ccagcatgga aaggatcaga
    14341 aaaagctcac tcatataaaa ggaagaggcg gtaatcgaca gaatgtagtg tgaatatagg
    14401 ccaccagcaa ggccgccgat acatgcgctt atcacataac caaaatagcg caccgcgacg
    14461 aagttgatcc cgctggagcg ggcggcatga cgatcatcac gggttgcccg cagcttcaaa
    14521 ccgagagacg attccttaaa ataatagaca gcgatgatca cgagcaccgc tactccgagc
    14581 gctacccaga gggtagtcag acgctgcgtc ccgccaagag tgaaggttct cggaccatta
    14641 gttacttgtg accattggga aagcatcaca ttgatcacaa ttagcagcgc aaactgagtg
    14701 atcgctccaa ccgcatcgga taggcgcatc atgggccacg caacgatggc tgctacgatc
    14761 attgccacca cggccccagc tgccagcgct aggggaaagg gtaagtgcag attgattaaa
    14821 aaaggataca tcgtaggaat ttgcatagct ttgactgccg gcgtcataga gaagatcgcc
    14881 gaggtaaaag ctccgatacc cacatagccg acataactcc aattcaaaat tccagagtta
    14941 cccatgtaaa gctgaaaacc aagcgtcata atcagcgtaa tggtagcggc agtaaagatg
    15001 tcctcaataa aggggatgtt tagccaccaa ataaagaagc caatggctgt aagaataaag
    15061 ataaggaaaa ccaccgttga agatttgcgg gtaaagtcaa agtattcgat aaaatctctt
    15121 acggcagacc gcttattgct ctcctgtttg ggggaaaaac tcttcgctga attcatccta
    15181 cacgctcctc ctggtagttg ccacgcagca gtccctcggg tttaagtacg agaaatgcta
    15241 tgacgattgc gaacataaaa gcatcacgga aggacagtaa cgaggaagga agtgttagcg
    15301 agagaacgtt agctataagt gcgtagacaa acccgcccac cactgacccg ggtagtgaat
    15361 gcatgccacc gatgaccaca gcaataaagc cgatcagcaa gggtgtcgcg cccatcatgg
    15421 gttcaacgga gcctgtgcgc gcagaccaaa aaatagctac cacgccggct agaaaaccgc
    15481 tgatgatgaa agctaccgaa atcatgagat ttgctggtac gcccatcaga cgagcagtga
    15541 caaagttctc ggaggcgccg cgcatcgcca cacctagtgt agtatatttc atgataagca
    15601 caagggctgt cagcagcaca acggtagtcc cgataatcag gaggttgcgc agcggcgtga
    15661 tcaccccgag tatctgaata ttcatattga aaaatgccgg aataggtaca gctttggaac
    15721 gcgggctgat ccctaattga aagaggcgct gcaagataac gcttaccgca aaagaggtga
    15781 ccagcaaggc gtccagtgaa cgttcacgaa aggcccggaa ggctaccagt tcgcttaaga
    15841 aaccgactgc tacgccggag gcaataccca atagaaaaac cactggccag ggcagactgg
    15901 tgaaagcacc aaggaaatac aaaacatagg cacttacggt aataaattcg ccataggcat
    15961 agttgaccaa ttgacaaatg ccaaagacaa ataccagacc caaagagatc aaggcgtaga
    16021 gactgcccat gcttagtacg ttgatggcaa actcaagcaa tatttctgca ttcattagac
    16081 atctcctcca ttctggccgc caagatacac cgatgtgata tccacgctgc cctcgaggtc
    16141 ctgaggtcgg ccggaatatt caatttttcc gttggacaac aaataagcac ggtcggcgat
    16201 gccgagagct ctttgggcat tctgctcaac cagcaggatg gtgatgccgc gggacttgag
    16261 ctcaatgatc agctcgaaaa tttgatcaac aagcgcgggt gaaagaccca gtgacggctc
    16321 gtccatcatc agcagatcgg gatttgaaat caatccgcgc gcgattgcca gcatttgctg
    16381 ttcgccgccg gaaagcagac cgccggcctg atgataacgt tctttcagaa tgggaaacag
    16441 ggtgaataaa tcctctaggt ctgcggcgta actcttgtct aaacgacgtc caaaggcacc
    16501 gatgcgcaga ttctcctcta ccgtaagcga cgggaaaatc tcgcgacctt ccggcactag
    16561 ggccatgcca agtttatgga gatgttccgg cttactgccc gtgacgtctt tgcccttaaa
    16621 gcgaatgctg cccgatcggg gcttcagcac acctgctatt gcctttaaga cagttgattt
    16681 gccagcaccg ttgacaccgc agatgctcac caattcacct tgatggactt ccatacagat
    16741 cccgtggaga gcctgtatgg aaccgtaggc cacgtaaaga tccgagattt caagaaccgg
    16801 gttatttgtc atgatgcagc actccccaaa tacgccttca caacctctgg atttttacgt
    16861 acatcctcgg gtataccctc ggcgatggtt tttccatagt tcaacacgtg caggcggttg
    16921 caaaggttca taattaaact catatcatga tcaacgatga tgatgccaat cttcttgttg
    16981 tcgggcatcg atctcaagag agacaacagc ccatccgttt cctcttcgtt caggccagcc
    17041 gccggttcgt caagcagcag gaatttcggc tgtgctgcca aggcgcgcgc aatctccagc
    17101 aaacgctgat cgcgaaatgt caagccagaa gcaggcagat cgatcttatc gcccagtccc
    17161 agttccccta atagtgcgac tgctaggtgt gccgcttcac ggcgttttgt acccacacca
    17221 atggcaccaa tcatagcatt ttcgagacag ctgaggttct ggaagattcg gatagtctga
    17281 taggtgcgta ctaaaccata gaaaggaacc cgataggcgg gctcatcgcc aatattgtgg
    17341 ttatcaagca atatctgtcc actgtccttg gccagcatgc ctgtcatcag atttatcaat
    17401 gttgttttgc ctgaaccatt cggcccaatc aggccaagga tctcgccagt ctcaaggtgc
    17461 aagctgacat catctacagc tttcaggccg gcaaaagact tgcttagcgc tctcacgaag
    17521 aaggtagatt tactttccat aatgtcactc tccaacactg caagcgtgct ctatcaatag
    17581 aacacgcttg ccttttatct caaacgctgt tttgtcaatt atatcttaat ctacacatga
    17641 gtatctcaat cgatctaact tttagtgggt cttacggccg tggcaggtaa tcgggggtta
    17701 cccattcgag aaagacagac ttacctttta caagttccat tacagcagca gccttgtttg
    17761 gttcatggcc gcttgctgca tcagaccaat ccagcttccc tgtcagcaat tcgaaggtag
    17821 tgtcttccat atacttggcc attttgtcgc cttcgaggcc accttcacgc atggcttcgg
    17881 cgagtaccat tacgacgtca taaccagaca tcgaccaagg agcatcaaca tccctgccgt
    17941 gtaattcttt ataagctgcg ttgtattcgt taaatccggg taccgcggct tcactgttat
    18001 agctgtgaga atcccagaaa atatcgttgc caaagtctgg gccgagcagg tcgaaaagcg
    18061 ccgggtcatc ataagcatcg ccgccataaa tcggagaatc gataccagcg gtgcgaaact
    18121 ccttgatgat cgtgcccagg tcgggcatat aggagctgat gtaaatgaag tcgggagttt
    18181 ccggcagagc cttgtagtga gcaatcagtg cagcagtgtc tgctttgcct tggctgaaaa
    18241 catcctcccg caatactgta ccgccaagtt tctcgtagca ttctacaaag tagcgtgaaa
    18301 ggctcttggt gtaatcgatg aaatcatcgg tgaccacata gacggttttt aaaccatcct
    18361 tcacataggc tttttcagcc attacagcac ccatggtggt gttccacatg gacagcgtga
    18421 actgtttgtc accaagcata gtgctgccga ataacggcga agaagcactc gggctgatgc
    18481 caacaatgcc agcttcctga gcagcgcgag ctgccggacc gccgaagtca taatccgagg
    18541 aggtaatgat agctgcagca ccctggtcga taagctgctt ggctacttcc gcggctgttg
    18601 ccgaatcaga tttagagtcc atattgcgga actccaacat ctttccgtcg attccgccat
    18661 ttgcgttgat ttccttgatg gccacttcga taccttccgc agccggagca tctagtgggc
    18721 cttggatgcc tgtcatagac atcgcgccgc caatgataat tgtgtcacca tctgtctttg
    18781 gtccagtctc ttccctgcgg caccctgttg ctgtgatcat taaacccaca gccataagaa
    18841 ctaccagcag cagaaagata attcttcgtt tcataattac acctacttcc aaattatttt
    18901 atttttttcg ggcccccacc cgttgacacg cagaactggc ttaaatttaa ttttcttttt
    18961 attgtaggac agttttaatg tggtgtcaag ctgaattgtt aattctctga aatagccaat
    19021 gtgtgacaaa attcatttaa ataacatgca aattaaccac ggtggctgaa aaccgtggtt
    19081 aattgattta ttagttgatc tttttaagat cattacatat ttatttactg atctacaaaa
    19141 acttattatg agactaaaaa tgctatgcgg aggggttatg acaaaaaata cgcctatcac
    19201 aaaagattgg ttaatgctag gaatgttaac aggtgtgggt ggagcaatag caaaagaatt
    19261 agttaattat ggtctattta aaaagaacat tccaacagtg cgctacgcca gtattgcggg
    19321 tggtgctgtt ctaggcaaaa gggctacatt tggcataata cccaagaaac cacgtactac
    19381 agctgaatgg gccattggtc atgttgggga tatagttctt ggcggcttgt ttggcagtac
    19441 cttatcatat gtgatttcga aaagtcctcc tggcaatgaa attacaaaag gtataggtgg
    19501 tggggtgacg ctttgggcaa ccactctagc atttggaaac ctcttgaatt tagatggttt
    19561 agctcgtatt aagccaaatc aaatggcatc tttactcgct gtgagtgctc tcttcggcgg
    19621 gacgcaggga tatttccttg gcaagtatag agaaggatta attgaaccgt catacccgat
    19681 taaggtacag gaaactagtg atgaccaata caaaagtaga agaatgggaa atagacgtac
    19741 tagaaacatg gagaaacata ccaatcaacc tatcatgcat taaaaggggc cgcagaggtc
    19801 cctttatcat ttactctgta gagatcatgt ggcctcttac taagctgaat aattcctgag
    19861 atgtatacca aatgtatatt ttagcctatt ttaaatttgg acgcaaaaaa ccacaggcta
    19921 caaagacctg tggttattgg cttatgatgg tcggagcgac aggatttgaa cctgcggcct
    19981 cttgaccccc agtcaagcgc gctaccaagc tgcgccacgc cccgacacaa atgttattat
    20041 agtatattct acagaggatt gcaagtgtga cattgtggta tttttaagcg ggttaatccc
    20101 tctttcttct gttatttgct ggggagatat cgtttgcgag ttataataat tgtctattgg
    20161 cttttattct tacaagatcg taagtctact atctactgct gaaattattt attgtgatat
    20221 actggaaggg aattttagta tatgaggtat taacaatgga gactaaatta ctacttgttg
    20281 aggatgatct ttttttgcag gatggcttgt gtgaactgct gcaacgtgaa ggctacagtg
    20341 ttaattgtgc cgccacctac aaagaagcgc tgtattttgc agcgaagcat acctacagcc
    20401 taattatttt agatatcacg ctgcctgatg gcagcgggct tgagctgtgc gaacagtggc
    20461 ggctgcatgg taaagataca cctatcctct ttttaacggc gcgagatgat gagattcaaa
    20521 ttgtgcgggg attggatgcc ggtggtgatg actatgtgac aaaacccttc cgccttttag
    20581 aactgctctc ccgcatccga gcacttttga gaagaaatca tccttcattc taccagattg
    20641 gtgaactgca gattgatatg caaaaaatga gtgccctcta taaaggtgaa cctttgtatc
    20701 taacgccaac tgaatttcaa cttttagcgg tcttaattcg caactccggt aaagtgctga
    20761 cacgcaaaat attactgcaa actatctggg atgatggcgg acattttatt gatgataata
    20821 ccctctctgt acatatcagc cgtctgcgtg aaaaaatcgg cagttcctat attcgcacgg
    20881 ttcgcggtat cggttatcga tgggaggaca agccatgcag ctaatttcat tattgtcggg
    20941 gatattggct gctctgggag cagctatctt tttttacagc agcagcctta ttaaatgggg
    21001 agggctgggt gtactgatgc taagcatggg cgccttgtgt ctttctgttt ctgtgcaaag
    21061 acggcaagaa aaagaggtta ctagactaca caggcaaatc atggattttc tcacaagtca
    21121 ggataaaata cctaacttta gtgtcaacga cgatttgttt gcagtattgg agaactccgt
    21181 tgtagagttg gaaaacaaac ttttattaga gcatgacaat acccgcaaaa caagcaaacg
    21241 taatgcagat ttcattacag atgtttcaca ccagctcaaa acaccactga cagcattaaa
    21301 attgtattgt gagatggatc aaactcagaa ttttggcaca cacagccaaa aacaactggt
    21361 actgattgaa cgaatggagc atttaatcta ttctctgctg cgtttagaaa agctgcgtgc
    21421 cgatgtctat gagttgcaat ttgccaagca cgacttatct cagctattgg agcaagtctg
    21481 ggatgaatta aggctattgt atccaaagaa aaaaataaat atcgttggta cagccacatt
    21541 gcgctgcgat gcttactggc taaatgaggc aatgatgaac gttctgaaaa atgcttgcga
    21601 acacacagct actgatgggc aaattcaggt tttgttagag actacagaag cagctgtaac
    21661 agtgagcata gaggataatg gtggaggcat cgcggcagca gagctgccca agttgtttca
    21721 gcgtttttac cgtacagctc aaactaaggc cagcggtggc gcggggattg gcttggctat
    21781 taccaaaaca attgtggaaa agcatcatgg cacgatctat gcggaaaaca tgggacccgg
    21841 cttgcgtatg acgatgtgtt ttcccatctt ggacggcgtc ttggcggctg gttagcttac
    21901 agaaacgtaa gcttggcgtc acggcaaagc aaggtacaac tggtagcata aggtcaaggg
    21961 ggtaatagta tgcaaatatt acaatgccag aatctctgta aagactacgc cattggcgaa
    22021 aacaaagtac atgccatacg cgacatttcc cttgcctttg aacagggaga gatttgtgca
    22081 atccgggggc cgagcggctc gggaaaatca accttgctgc atattttagg aggattagag
    22141 tatcccacct ccggcaaggt tcattatcaa aacgaggaac tttttgctta taatgataat
    22201 cagctttcca ttttacgacg ccggcgcttt ggctttgttt ttcaagctta caatttagta
    22261 caggagctga cagcgtctga aaacatttta ttacctgtga tgctggataa aaaaatgcct
    22321 gatgaaaaat atcttgccat gctaatcgaa ctgttaaaac ttgacgatag gctggatcac
    22381 ctccccagcg ccctttctgg tggtcagcag cagcgggtgg ccatagctcg ggccttagct
    22441 aataaaccct ctgtcctttt tgcggacgaa ccaaccggta atctagacgg aaaaacgggg
    22501 cgtgaagtcc tctcactgtt gaaatacgcc agtaaagaaa tgggcgtcac cctgattatg
    22561 gtgacacatg atcttcatat tgccgaacag gccgaacgta tcatcttact ggaagatggg
    22621 cttatcacac aagatagcgg tcagcgggag gcttagcaga tgaagtctac attttcgctt
    22681 aatgctattg ctttcggtaa tcttcggcag cgacgcgggc agtacgtgct gctggtgatc
    22741 ggtattgtcc tagctatcta ctttgtcgcc accatgctgc tatttgcctc gaccatgttt
    22801 atttcattac aagagcggca gtttcagcgt ttgggagaac aggacgcgat tatctttaat
    22861 acacgggaga caccattgtc tgagctgaca gaaagaggcg tttttgctga atatggggta
    22921 gctgccattc agggctatgt gctcccagat ggacaaaact ctgctaatgg tttttcggtg
    22981 gcagtttttg atgatgttgc gctgcagcta gcacgcaaag atgttcttca gggcagacta
    23041 cctgttcagg caggggaaat cgcactggag caaagtatgc tggctcgttt acgtacaact
    23101 gcagctgtgg gagacaccat cacgctgacg ctccaaattc cggatagcgt cagctttatg
    23161 cctgaaacaa ttatcaaaga ctataagtta gtaggcatct taacagataa actggtttac
    23221 ctggagcaaa gaggagcctc tctgcccgct tactatgact accctgccgg ggtactgtca
    23281 gccgctgagc cgcttacagt cggcggcaaa gaggtggtga atgtctacgg acgttatctg
    23341 cctgacgccc gtgagtctta tacaaaaatg gatacttttt gtgcagagat gggactactt
    23401 gggaaagatg gccgtccccc ttacgatatt atgcattatc agctgtttga cagcagctat
    23461 aacaacagcg acatcgtaac aacctatgtg attttgatca tcattgccat agttttagta
    23521 gcggcagcgt gtttgggaat cataaatgcc tttgccgcca atctggaagc tcgtaagcga
    23581 caaattggcc tgctgcgggc agtcggcgcc actaaaaagc aaattaggca aatctttggc
    23641 aaagaagctt tgcttttatc cctgttttcc attccgctag ccctggtgct cgcctaccta
    23701 acagtgtggg gaatcactac agcgatgggt gatagtttcc tcttgcgacc caatttactt
    23761 atcttaatta gcgttgcagc tgccggcatg ttgtgcgtaa agctcgctgc agctatcccg
    23821 ctcaatactg cgtcccaagt accaccgatg caggccattc gcaatgtaga attatctcgc
    23881 aaaattaaga taagcaaagt cgccagtaaa aagagctttg ctgtgcccca tttactagca
    23941 caacgcagtc acacacttta tagtaataag caagctgcta tcacagccat gttagcggtc
    24001 agtatcctgc tgatctcgct ggcagttttg tatgtaccat ctatcctgga gcaggcaacc
    24061 tttaactatg gcagtgattt tcttctctta gactacagat acgacgacga ttggctgatg
    24121 gcatacaatt ttcatactcc cggcatcact gagcaagaca ggcaggacac tgccgcactc
    24181 ccaacagtag caaaagtaac aggagaaaaa aagcttagtg taaagatctt aagcaatacc
    24241 attacgccat atgtgaccaa tctatgggag tttgagtacc tacttgcaga gccacggcat
    24301 gaagataata gtgagtacgc agaagcgtca cgcgtgttca aagccttaca gcatcaaagt
    24361 taccttaaca gcaaggaaaa gtatggctat atgcaggatt atcttacggt aagctgttac
    24421 gcaacagagg cagatgtcgt acaaaggctg accccatatg tagcgtcagg taggattaac
    24481 ttggacaaac tagcctccgg tgaagaaatt ttgctaatag caccacctga gtatggtttg
    24541 tatgaagaac agtataatga tggcggctgg actcgccgct tggattacat ccctgatcct
    24601 gacgtagatt atcagggcat ctatcaaaac gatatgtttc aagcagggga ttcgcttagc
    24661 atcagccttt tatatagtga tatatccgaa cagcagcatg ccgatggacc acaacagtac
    24721 aataaagatg gcacacgcca actgccggag gacgcagtac gagtggacaa aacagtttcc
    24781 attggtgcca ttcttcagcc tcttgtcgat gggaagtatc ttgacagcaa ctttagcttc
    24841 atgcacacag aaattggcga tatcctgaca acaactactg gcctgcaggc tctcggcttt
    24901 gatgttccct atgccagcct gaatatcatg ctggctgcca gcccagatga agtaatggaa
    24961 gaatatctta cagcaaacct ctccggcatt gccgcacgca cttctggagc agaatttaag
    25021 tcgtatatcg ccattgccag agaaaataga aaaatggctt atggtattgt aattgccgtc
    25081 tatgcagtgc tgtttctctt ctttgccata tgcaccagta tgattaacaa tgccctctca
    25141 gctcgcatcc gagcaagcaa gcgtgaaatc ggcactatgc gggcagttgg ggcgtcagag
    25201 caagaaatta tgctcactta tcagtggcag ttgagtgtaa tgttttgctg gggcacaatt
    25261 atcggtatgg tggcacagct ggtagtatgc acttggatac tatcgataga cccactctgg
    25321 ggccgagtcc gccctacccc tccagtttgg cagccgctct tgtttgtggc actgctgctg
    25381 ggaatctgct ctcttagtgt tcgtgctaaa gtaggcagta tcgtcaaaga cagtattatt
    25441 gccaacattc gtgagttgtg aggaggagcg agatgaaaaa aaatctattt ttgctggtct
    25501 ttgtcctatt tatatgtaca gcacctctcg tctctgcaga agaaacaacg gatctgaaaa
    25561 atgataacct aacacccgca atcggagctg cgggcccggc tttatcaatt gatagtgtgc
    25621 ataagtatga ggggatggga aaaagttacg ctgaaggtta tctgcctgct gtcagtgaaa
    25681 acaaagcaat ggtggtgctt ccgctattag caccagaagt aagagataac ctgacagtta
    25741 cagtaaattt gggtacgccc gcggatactc cttttgtgtt taagaattat cagaaacagt
    25801 ttactagcca gagttatgtt tttgaggagc aagaggtgga ttgcttttta attcagtttg
    25861 ccctccccct gtcggctgaa cgcctcaatg ggacttaccc cttaatccta acggtaaagg
    25921 gtaacacagc ggacggagaa gcctttgccg aagattttac gctgtacctt acgataaccg
    25981 acggcatcaa ccctctggca ccaacacctg agccagcgcc agctccctcc tctcagccca
    26041 aattgatggt agaaagcttt gctttgtcgc aggactatct agcagcaggg gagcaagcta
    26101 aattaaaggt tactttacgc aacaccagca gctcacagtc ggtaaaaaat atcaaactct
    26161 cttttaccga aaacagcggc gaaatcttgc ctgtggctac aggtgccaag tactgtaagc
    26221 agatctccaa aggcagtact tatacttgga atttcgaggt gtcggccaca accacagctc
    26281 agtcaaaacc tcatccggct acaattacca tggaatacga agacaatcgc ggcaatgctg
    26341 tgagcggtgc cgatcagatc attatccaag tccgtcagcc tgtacgttta gaatatgaag
    26401 aaccaacgct gccaacgcgg gtaacacagg gggacacacc atcttttagc atgacactga
    26461 tgaatcttgg caaaagcacc atctttaatg cgctgctcaa gtttgagctg ccgggattaa
    26521 gcagtggcgg cagtgtgctg gtagggacca ttcctcccgg ggaaacggcg gtaggtcgcg
    26581 ccaactttcg tctcgatacg gatgcactag gagacgtatc aggaaagctc tgcttatctt
    26641 atgaggatga atacggtgaa cactatgaaa gtgagatacc tcttgccact accattgaaa
    26701 agagactaga gcttgccgca cctgccgata agaaggaaac tgcttctagc agttttccct
    26761 ggacaatcgc catagttagc ggctgcattg ttctgatcgc cctgctctcc atcttcggct
    26821 cccgctggct gcagcaaaaa agagctcgtg cagaggatga aatgaggctg taactacccg
    26881 ctgttaagca caacccccta ccgctatgca ctgtggtagg gggttgtagt tttttaatca
    26941 aatatgcgac agttgctgat gaataaccgt tgcccgcttt ccatgttagg caagaaaact
    27001 attgcaaagc gcgcgacaaa gaaccgtccc ttgtcacgcc ttacggtgtt ggttggttag
    27061 ccaaaaggag agaactaata agaaaacggc tgttaatacc ccaaccatat caatgcagac
    27121 atctacatag ttgccgtggc ggcccatgac aaaaccttga tggaactcat ctaaaatggc
    27181 aaagatggta ccaccaagga aggaacttac gatggcttgc caaggttggc ggaagtagtt
    27241 gcgcagcagc aaaaaaaatg ctaaggttat gacaaaaaag aaaaagacat gggcggcttt
    27301 tcgcaacaga aactcaataa tcacgggatt tcttctggca tagttaagga agtctgcagt
    27361 taaagttttg gccggtgcca gctgtgttgg tagatgggtg gcaattttaa tggcaagttt
    27421 tgaaatggtt aaatcaaagt attttaagag gttattgatt tggcgcaaga caggcagcac
    27481 acgcagacta gggatggaag ataaataata aagtgtgccc actgcggtca gtagaacaaa
    27541 ccaatttctt ttacgcatag ttaagagctc ccttaaacgt tttatatatg attcgacatt
    27601 tacctgttaa gtccttgttg ggagcttcac ccgcctcacc agtcccgctg cctatgcccc
    27661 tgttaagtat caaaagcgtg acaaggaaat ttggcaataa aaaatgcgtg gtaaccacgc
    27721 attttttggg ttaaaggatg atggcgataa gaccgccgtt tccttcgttg attatttttt
    27781 ctagcgtttc ttgcagcttt tcttgagcgt taggtggcat gttatccagt ttgatgctga
    27841 tgccatcttg tactactgag tataatgatt tgccgaaaat gtcagactcc cagatttttt
    27901 ctgggctttc ttcaaactca tccataatgt agttaactag ctcttcgctt tgcttttctg
    27961 tgccgactat cggtgaaaat tcggatttca catctacacg tacgacatgc agtgaaggtg
    28021 cgctagcacg gagccgaaca ccaaaccgac cgcctgaacg gacaatttcc ggttcttcca
    28081 gtgtcatctc ttcaagcatc ggaggtacga taccgtagcc tgtttcacga acttgttcca
    28141 aagcaccggc aattttatcg tattcgcgtt tagctgccac actatcttta agaattctca
    28201 acagatctgc ttggtcatca atatcaatgc cgcagatttc tgaaaggatt tgttcaaaga
    28261 gctcttctga agcttgaatg tcaataactg ctctaccagt accgagatcc atttctttaa
    28321 gtacaatatt ttctacgata tctttggtac taagtgtttc taccatgcca tcgacatcac
    28381 ggagacgggt tacttcaatg acactttctc gtataatgtc gttgtagttg ttacgtaacc
    28441 agtgttcatc ctccagtact tccacccagc tgggaagatt aatgttaact tcacgcacgg
    28501 ggaattcata cagtacttct tggaagataa cattaatatc ttcttctact agctgttgag
    28561 cattaaagga gatcaccggc acatcatgtc tatctgttaa ctcttccttt aaagctagtg
    28621 cttcctggga gtacggacga gctgaattga gcagaatgac aaagggtttg cccaactctt
    28681 ttaattcggc gatgacgcgt tcttcggcat caatataatc tgtacgggga atgtcagcaa
    28741 tggagccatc tgtagtcacg acaataccga tggtggagtg atcagtaatg acttttctgg
    28801 ttcctacttc tgccgcttcc tcaaagggaa tgtcataatc aaaccatggt gtagttacca
    28861 tcctgggggc accatcatcg tcataaccta ccgcacccgg cacagcgtaa ccgacgcaat
    28921 caactaaacg tacgcgcatg gtaatattgt cgcgcacttt aatctctatg gcttcatccg
    28981 gaataaattt cggttctgtc gtcataatgg tgcggccagc gctgctttgt ggcagttcat
    29041 cacgggcgcg ttcctgatct gcaggctctg caatattagg aatgacaact agttccataa
    29101 acttttttat aaaagttgac ttgccggtcc gaacaggtcc gacaacgccc aggtaaatgt
    29161 ctccaccagt tctttcaaca atgtgttgaa tcaggtcaaa tttaaccata ttttctccct
    29221 ccttttttca agcctaacca aaacactaac ggtatccctc ccctgtgaac ttttaaagtg
    29281 ataagttatc acaacaaaat aaaaaattgc agtttaacat tatatttata tgagcacgta
    29341 caccgaatat tacttttgcg gagggaaatt acggtaaaat aaataaaaaa gcttgcactt
    29401 tttccatata tctgcttgca agctattatt tactggtttg attagcatgg aaagcataaa
    29461 taagtcagcg ctttcggttg cagattgtaa gctttttatc ttagccgcgg gcggaacaga
    29521 gataaattct ctgcagtaaa aaaaacactc catgttggag tgtatgctac caatctttaa
    29581 attttgacga tacttgttct tccatttcgt atgttttttc ccgggataag aggtcgacca
    29641 ctgcttgatg tggatctctt tcggcaaaga gcacttgata aacttgctct gtaatgggca
    29701 catcaacatg atatcgctgg gctaaatccc aggtggctcg agtagtacgg acgccctctg
    29761 cgaccatccg catcccccca ataatctcgt tcagcttacg gcctttgcct atttctacac
    29821 ccagactacg gttgcggcta tgacgtgaag tacaagtaac aactaaatca ccaatccccg
    29881 acaacccggc gaaggttaaa acctctgctc ccatccgcat gcccaaacgg gcgatttcgt
    29941 taatcccgcg ggtcattaaa gctgcctttg tgttatcacc ataagacaag ccatcagaga
    30001 tccctgctgc cacggcaatt acatttttta gggcgccgcc taattccaca ccaatcagat
    30061 ctgggttggt gtagacacgc aggcgatgtg tcataaatag ttcctgcacc gtttcggcga
    30121 ttcgccgctc gcgggaagaa acaacggtga cagacggtac aaaccgtccg acctcttcgg
    30181 catgatttgg tccggacaac acagctagtt tacaagcggg catttcttgt tcaataactt
    30241 cagacatgcg aaacagtgta ccttcttcta atcctttggc acaactaacc atgatcggag
    30301 agttattaat cagcggcgca atctgctgca caatggagcg tacagtatgc gagggcacca
    30361 ctaagacaat ggcttcctta ttgtgcacag ctgcggctag gtcagaagta agcacaacat
    30421 tgtccggcag gataactccc ggcaggtagt ctatattctc tcggctggca ttgatttgct
    30481 ggcagagttc ggcccgtcgt gtccagagtg taacctggtg acaattttgt gctagaacaa
    30541 ttgcaattgc tgtgccccag ctccccgcac cgataactgt tacttctttc atttcttcac
    30601 ctcattcccc atattcgctt tcttgcctaa cttacgttct gttccccgca gtaagcggga
    30661 aatattctct cgatgacgcc aaattgccat catggcaatt cctgccccaa agataagata
    30721 caggggcggt aagtgaaaat gaatcattat aattgggatt gccaacgcac cagtaatgga
    30781 acccagtgaa acatagcgcg tgagagcaat cagtgatacg ccgatgctgg ccgcaatcag
    30841 cagaactaaa ggagctactc ccagtaaaac accaatagag gtggcaattc cccgtccacc
    30901 gcggaaccta aaaaagagcg gccaattatg tcccacaatt gctgtgacac cagcggccat
    30961 ctgtacaatt tcactacctg tgagagctcg ggctatccag acaaccagca atcctttact
    31021 aatatctaaa agtaaaacga taatcgctgg catagtacct aaagttctct ggatgtttgt
    31081 ggtaccggca ttaccactgc caaagttacg tatatcgata cctttaatta gcttccctgc
    31141 caaataacca aatgagatgg aaccgataag gtaggctgcc agaaacgcca taattgtctt
    31201 cacttaacgt tccccctttt aactcctctt ccgcaccaac agcttgattg gtgttccctc
    31261 aaagccatat gtttcccgca gtttattttc cagatagcgc atgtaggaga aatgagctaa
    31321 ttccggatca ttaacaaaca acgcaaaagt tggcggcttg actcgaatct gcgttaaata
    31381 gtacagcttt aattgtttgc cctttttggt cggaggcggt gtcacggcta cagcatcttc
    31441 tagtaacagg ttgagctgag aggtttttac ccgcatggca tgctgctcac ccacataatt
    31501 aattaattca aaaagtcggt ggacacgctg accagttaaa gcagaaataa agacaatggg
    31561 cgcataactt aaataagcga gctgcttacg cactgtatca cgataagcgt ccatcgtttt
    31621 ttcatccttt tccaccttat cccacttgtt gacgacaata atcatcgccc ggccagcttc
    31681 atgggcaatg cctgcaattc gtttgtcctg ttcagcgata ccttctgcgg catcaagcat
    31741 cattaatacc acatcggcat cttccacggc gcgtacagaa cgtaaaacag aatagtattc
    31801 tactacttct tcaactttac tgcgccgacg gatgcccgca gtatcaacaa atagatattt
    31861 ttggttttcg tgctcgacta acaaatctac cgcatcacgt gtcgtgcccg gaatatcact
    31921 gacaataaca cgttcattac ccgcaatttt gttagttagt gaagatttgc ccacattagg
    31981 gcgaccgaca atggccacac gtaataactc ttcgtcttca ggaaactttt cccctgccgg
    32041 cagaaatttg ataacctcat ctaataaatc accagttccc aaaccatgcg aaccggaggc
    32101 aagctgcggt tcacctaaac ccagggcata aaattctgcg gcaacggctt cagactcact
    32161 agcttctgct ttatttgcca ctaagagtac cggttttttg gcgcgacgca gcatatccgc
    32221 cacctcataa tccagtgcta ctggaccact gcgtacgtct accatcatga gaataacatg
    32281 ggcttcttcc aaagccaacc ggacttggcg atttatttct ttagaaattg tatcctcttc
    32341 aaaagtcagg ccccctgtat cgatcagcca aaattcacga tctgtccatt ccacgcgtcc
    32401 gtagatacgg tcacgtgtga ctcccggagt actttcctca atggccaccc gcttctgaat
    32461 caggcgatta aacatagttg atttacctac attagggcga cctactattg ctacgacagg
    32521 catagacatg ccatcacctc atttcgtttc ccaacaacat tgttaaaaaa tcatgtacat
    32581 cctgaaccac tttcacttct gtctttgctg cagctgccag ctcttcagta gtatacccat
    32641 caagaaacag ctctcttcct tctttgaaca tcacagacgg taaataaagc cttcctttta
    32701 ccttattaat aactttattt aaatcatgaa aggttaataa ccctgcgaca gtaacttcgc
    32761 cgccaaaaaa gtgattttcc actgccaaca aagttaattg taagttttcg atctgattta
    32821 aacgctccac aaggggggcg aggtaaacgg cagccgacac accagtggca attgtcgctt
    32881 ccgtagcgtg agctaatttt gcaggcaggt ttttttcctg atctgcccag tcatctaaaa
    32941 ataaacgaac catgcccaca ccattttcta attgtcgata gccttcataa gcttcggccg
    33001 cagggggcat cattccggcc atcgtataaa attcatctga ggcaaaaaca aaacgtgttc
    33061 catgcgccgc taaactgcgt tcctgccaag cttgaagctg gtccaatact tggcgggcat
    33121 gggctttgtc acttgtttct aaagggtata aaccttgccg gtgtctagtt aacccaaccg
    33181 gcaccaccgc caaagtttgc acggcaggat ataaagcata taagtcggcg attgtctgct
    33241 ctagagcctc accatcgttt aaaccaggac aaagtactac ttgtgtgtga aactgaatac
    33301 cggccgcagc cagatgctgt aaagctgcca gcagttcccc tgcccgtcgg tgattcatca
    33361 gccgctgccg cagttcggga tcagtggcat gaaccgatac atacaaagga ctcaaatgca
    33421 aactgataat gcgctcaaaa tcttccttac gtaaattagt tagtgatatg taactaccgg
    33481 acagaaaaga cagacgataa tcatcatctt taatatacag agtttgccgc attcttgctg
    33541 gcatctgatc cacaaagcag aacagacagc ggttctgaca aatgtgcagc tgatctagcg
    33601 ttggggagtc aaaaacaata cccagcgctt catcataatc tttctcaatt tcatattcta
    33661 ctagttctcc accctgatgc tggatcgtta ataataattc ctcgccgctt tctgccagct
    33721 gccagtctaa aatatcagct ggctctatac catttacggc aatgacataa tcaccatcgg
    33781 ctatacctat ttcctcagca atcgagtcgg gttcaacccg ctccacaagc ttgccccgtg
    33841 tcatgtgcac acctactttt tctatacttt tactattatc ctacaacaca ggaggcaatg
    33901 tcaaatagaa agaaaaagtg ggaggtaata agatcttcag cttattttca aaaaataatt
    33961 ctttacagat tataagtttt gtggtactgt tacttttaat accataaaca aaaggcagtg
    34021 aaggagaaaa gtaaggtaga tacgacctca cagggaggag acgtcatcga ttgcaagcgt
    34081 ctccaggtac gatttgtctg aagttccctc cggagccgca ggttgaaaac tatgtaatat
    34141 gtagaccgtg ccgggaaccc cgtccgttac aaaaggggca gatatcggat ttatcctgta
    34201 tttgtcatag agtgacgcag tgcgtaagtt gggtggtacc gcggataatt atccgtccct
    34261 tttaagggga cggtttttta tttgcctaaa aatgcttagc agcaaataat ctatggaggt
    34321 ggattggatg atcgttgtga tggatttaca ggcagcacaa aaggaaatac aggcagtaga
    34381 agatgtttta caggagtatg ggtacggctg tcatctcata cgcggcgaaa tgcgggtagt
    34441 gattggtgcg gtaggttctg ctcagtcgga aataccgcct gctttggaga cgatgcctgg
    34501 tgtagagaaa gtgatgcgtg tgatacaacc atataaactg gttagccggg aggtgaaacc
    34561 ggatgatacc gttgttacgg ccggcagctt acgtgtgggc gggggtaccg ttgcggtaat
    34621 cgctggacca tgtgctgttg agagtcaggg tcagttacgc gccgcagcaa atggtgttaa
    34681 aattcatcag gcggctgcac tgcgtggtgg cgcctttaag ccacgcacat ccccttacgc
    34741 ttttcagggg ttgttggcta aagggttaac ctacttacgt gaagcaggtg aagaaaccgg
    34801 tttagctgtg gtaacagaag tggtgaaccc cgcggatgtg gaaatggtcg ccaatgcagc
    34861 tgatatcgtc cagattggtg ccagaaatat gcagaactat cccttgctaa aagaagtggg
    34921 gcagctgcgt aagcctgtat tactaaaaag aggccttgcg gctactgtgg aagaatggct
    34981 gatggcagca gaatatatta tgtccgaagg aaattatcaa gtaattctct gtgaacgagg
    35041 gatccggacc tttgaaacat ctacccgcaa tacattggat gtcagtgcca ttgcattggc
    35101 caaacagcta acacatttgc cagttattgc tgaccccagc catgcaggag gtcattggcg
    35161 gctggttaaa cccctcgcac tggcatcact tgcagccggc gccgacggtt taatcgtcga
    35221 agttcatcct gacccggccc gcgcactatg tgacgggaaa caatcactga cactggaaca
    35281 ctttgctgaa ctcatggctg aaattaaagc attcctgcct gtcttgggca aaactctatg
    35341 ctccggggtg ggaaatgatg cttaaggctg cttatttagg cccgcgaggg actttctcag
    35401 aagaagcttg cagagcattt tgccgtgtaa ccggactaat acttgctccc agcccctgtg
    35461 cttccattac cgcctgtgca gtgagtgtgg cagagggaaa cgcccgtttt gctattgtac
    35521 ctctggaaaa ttcacttgaa ggttcagtac gtgacacgct ggatctttta acaacaagtt
    35581 ctaggttaaa aattttagct gaacttgatt tggagatcga acatcatctc ctcacccacg
    35641 cggcagcact tactgaaatt cagacagtct attctcaccc acaagcgtta gcgcaatgca
    35701 gagcattttt gcatcaacag cttgctcatg cccaagtcat tcctgttgtc agcacagctg
    35761 aagcagcttg tcttgctgct gatcttggtt ctcaggctgc cgccatcgcc gggcagcagg
    35821 cagcgtcatg ctatagcctg cctattctgt atgccgccgt tcaggacaca acgtcaataa
    35881 cacgctttat tattctcggc cgggaagatg aaaatttcgg ccagcctcaa aaaacttctc
    35941 tgctctttgc tgtaaacaat acaccaggag atttgtatcg cgtcctgcga gcttttgcca
    36001 accagcaggt aaatttaacg cgcattgagt cccgaccagc aagaagtcgt ttaggagact
    36061 acatcttttt tgttgatctt gacggtacac cgcaggacaa agcagtaaaa gctgcactgg
    36121 atgaagccag ccaagaaaca gtgatgttaa aaatattagg ctcctttcca gtacttacgg
    36181 taagcgataa ataatggcac tacaggtttt gctgataatc gtggtccgtg tccaggtgcg
    36241 ggatatatgg cttgatatct agcacaggac tgttgtttag agcatccagc cctgttactt
    36301 tgatgctgtt ttcttttaca gagattattt tgcatataca taaagcgata ggattaggtc
    36361 tgttagggga tctgagagca aagacccctt tttcgcttgt tcttcccggc ggcacggcgg
    36421 taagtgtttc tctgtttgcc ctatcacccc agtatagtac gactatgtga ctccgttctt
    36481 cgatcccagc caaaccggca gcatattctg gcaaaatttc aattgtagat acttgctgtg
    36541 aatatttccc ttgtcgtggg gcagctcctt tatctgtgta agaactgtgt ataattccaa
    36601 ttctttttaa ttccataata taagctcctc ttcatgatct gcttctccaa tctacattgc
    36661 aaagcacctt ccccgacttg cgagtggcat actgctttga actcacggtt acagtgggga
    36721 gcgctcaggg tttgcacctg attcccttta tactcagaaa agtacctgta tccctacata
    36781 tttaatactg catataggtt accaaatagt gcgattcaat gcaaaatcta ttttttagag
    36841 tactaaacgt ctcgttgccg ccgtacttaa tgattctacc tctggttgag taaatctatc
    36901 acaaagcggt tgctgattta tcttccctat gcaatactaa agcaggaaag ggagatgaat
    36961 tactatgaca aattacgcta gcggacgctt aattgcagaa ctgcgcaaag caaagaacat
    37021 gacacagaaa gaactggcgg catatcttaa tgtatctgat aaagcagtat caaaatggga
    37081 acgtggcgag aactatcctg aagtctccat tttgccccaa ctggcaaata aactcgaaat
    37141 tacagtggat gagcttttaa gtgggaaaat tgctccgcag actgcaagtt cagctgacct
    37201 tcctgaggcg gagcaagttt atgcatattc ctgcttatta gaagatgccc gcttaagata
    37261 caacaaacac accttaacag cttatgccat attagctatt ggtatcctaa ccggctccat
    37321 catcttaaac aatattctcc cacaggttgc tgcggtggcc ttggctttga caggaacgct
    37381 cttttattat tttgccaata tccaatatca gaatggcaga gatagatttg tacagtttgc
    37441 accagcagct gttactgcag atgggacatc tgtcactctg ataaaatacg gtaatacttt
    37501 tatcagtacc actgctttcc tattactcag cattgctacc atagctgcac gcagtcctca
    37561 taacttattt attcaggaca actatctgtt gattgataag attatgccgt tccctgccct
    37621 tttgctgatt attatcatca atcttactat gtatgcatac ttttttaatg gctatagtag
    37681 ggatcgtttg caaaagatct ccagaggtga ttttgtcaaa atactaagca gcttctgcgt
    37741 ttcattaatc cttgccgcac ttgtgctaag cgttcagctc caaatgatta tcactaaggg
    37801 agcatataat tcagctttgg tcaataatgt aaccttactg gccttagtct tcgctgtatt
    37861 aaccaacttt tgcttactgt tctttaataa ccgccgcacc tttaaaaagt tcttgcacat
    37921 ttttgtcgta tctatgctgc aagccggtct tctcatctgt gcagcgaatc aatatgtcgt
    37981 ctccacaatg cattatgata ccaatgttct tggcctcgtc tacactggca atatcatcac
    38041 agtggtgatg cttgctttaa caatcttcca ctttgccttt atacaactat ctcgaaaaaa
    38101 atacgcttaa agctttattt ttgctgccgc cagcgagctc tgcgccaaga agaagttaga
    38161 aagccgcagc cccatagatg ggctgcggct ttgtcatttt tgcagttaaa atatggcagc
    38221 taaagtgtgt tatttggatc tgaaaaatta tacctacagt ctttcgcagc tccatcagcg
    38281 cctatgattg tcttgtacga tgctgctatg atattaatat gtcccttgcc aaaaacgctg
    38341 cccgccattc tagagtagaa gacataagtt ttgatttctg atcagctcaa ttcttttaga
    38401 taatctgtct ttgctttctg ctactgctta agggcagaaa aaatgggcaa ttatgttctc
    38461 tgtcagcaat tactttagcc tcaatcttaa agacatcacg taacatttgt tcagatataa
    38521 catctgctgg ggctccgtca tgataaatac ccccgtcttt aagtactgca attctgttgg
    38581 aataacgcgc ggcctgattt aaatcatgta agatcatgac aatcgtcaga gataagcttg
    38641 aatttaattt ttttactaac tctaggatct caaattgata acagatatct agaaaagtag
    38701 taggttcatc caacagcaag atttctggct cttgggctag tgtcattgca aaccaaaccc
    38761 gctgccgctc tcctccagac aaagttgaaa ctctgcgatg ctgcaaatcc gttattctcg
    38821 tctctgcaat agcccaatct actacttcct ggtcttcgct cttaaggcgt gagttccacc
    38881 cgaggtaagg atagcgacca taagaaacta aatcccttgt tgtaaaatca tcaggcactt
    38941 gtggcatttg cggcagcatg gcaatctttc gggcaactgc tcgggtattt aagctaaaga
    39001 gtgattgatt ttctaaatag attgtgcctg acgtaggttt taggtttctt gctaatgctt
    39061 ttaaaatagt agattttcca gaaccattaa taccgatgat cgtcattaaa tcccctttgt
    39121 ttatcactaa acttaggtta ttaataattc ggtgatcatt gtaggctaaa gtaatgtttt
    39181 cagctcttaa catcgcgagt taatcctcct ttcagtagat ataaaaagaa cggcgctccc
    39241 aacatagcca taataattcc tactggcact tctataggat ccattgcaat tctggccaca
    39301 gtgtcacaaa acatcacaag cgttgcccca aagatagtac ttgccggcag cagataacga
    39361 taatctgaac caataataag acgaacggta tgcggcacaa ttagaccaac aaaccccagc
    39421 attcctacta cactcacagc actggcagcc aatagcgatg ccagcgcaac caaaactaac
    39481 ctcatccttt caacattgac acctaaattc gtggcaattt catcgcctaa ggccaaaaca
    39541 tttagtttat tagccaggat agaaacaagt actagcccta ccaaagcgta tggccataat
    39601 aaagagaaat gtttccaact tctcgcggct aacccaccta ccataaaatc aatcacccca
    39661 tgtactctat tggggaaaaa caccattaag gcattaatcc aagcacttaa gaacgaagaa
    39721 acagcgactc cggccagtac catccgcatg ggtttaatac ctcccttcca cgctagtgca
    39781 tatattaaca gcgtggcagc taatgagccc aaaaatgcac aaggtataat taggtagtaa
    39841 tgcttgggca ggagcaccag aataatagtt gcagctatac ccgcacctga ggaaacacca
    39901 ataatgctgg gggaagcaag agggtttttc atgactccct gtagaattcc gcctgataga
    39961 gataaacaga tgcctactaa accggctacg attgttctgg gcaaacgaat attccaaatg
    40021 atttgtctgt tcgtagtttc tgtcttatta acaattgcct caattacttc tggtatagta
    40081 attttcaatg atcccatccc aacactaaag agaaaggaga gcacgcacag cactaatagc
    40141 aggaggatca cggcaagcct aaaactattt ctttgtctca ttagtttaat actttctacc
    40201 tggtgtgcaa cattcatttt aattttctcc atttaatcaa gtgtaacgcc tggatagagg
    40261 atattggcaa gataagcgaa ggcttccgga tatctaacat tcggtttata cataaacaaa
    40321 tccttaggaa gatagtgaat gttgccttct tttacggctc taatgccagc ccaggcttgg
    40381 tttgacgaca catcctcctt aatccttgcc ttacattcct ctagatcacc cattgtggta
    40441 attaagatca catcaggatc gctttccaca attttctcca gactaaagtt aacccttaca
    40501 tcaccttcgg ctagtgtatc ggtaacaatg ttttctccac ccaagatatc taccatgtta
    40561 ccagtttgtg cggttggcag ctcgcaggtc actgatttag aagaagcaaa aaggatcaag
    40621 actttaggct tgcgttcatc tactgtcttg tccacaattt ccttaacttc atcatcaagt
    40681 tgactaatat cggtctcaaa aatatctctg cgcccagtga ttctagtaaa tacatctaga
    40741 ttctcaaaga agtcttggta atcatagaat tcacagtaga gtgtctctat tttattttga
    40801 gcgaagacat cttttatttc acggtgagca gccattgtac tggtcatgat aatcaaatct
    40861 ggttgcatag caataatttt ctctaggttc ggactgccga cactgcctaa tatttctata
    40921 ctttctgctt ctggaggcac attctctgtt ccactcactc tacccactgc tgtgccaccg
    40981 gcaagatacc ataaatcaag cagcgagttc atgagaacaa caactttttt tggttcctta
    41041 gttattacaa cttcattacc taacggatcc attattgtaa tgctgcgttc atcttcagct
    41101 ctaatcagtt caggcgtttg atagtcgtta ttggcagttc cctcacaacc aaacagcaac
    41161 ataatcaaca caacacacag cgaaaagtaa acgcaaaact taatttttct tttcatttaa
    41221 atttacctcc catacaaact ttaatatttg ccgcactagt aacatgatag tcacctgtaa
    41281 taaaacaaaa cattacccct gcttcctgct gcacaaggct tctattaatg tctgattttc
    41341 agtatggttt cggactgcta cacggacgta gcgctggtta agtccctggt agttaccaca
    41401 gtcacggatg agaatcttct gtctgagcag ctctgcctgc aattcaactg aagctgcctg
    41461 caaactttcc aacaatatat agttggctcc aggagtaaaa ggataaatat ccggtatcgt
    41521 ttgtaaagcg aggtaaagct catgggcttg agcagtaatc atttctaccg acttttccag
    41581 aaaagtctta tctcgtagga caaattcgcc tgctaattgc gccaaactat tcacgctcca
    41641 tggatccctg tttttaacga tggcgtcaca aagctgcggc gcggcagcca aaaaacccag
    41701 ccttagcccc ggaatggcat acattttagt cattgagcag aggacaacta cgtgttcagc
    41761 tgccatttgc aggagagttc tctcctgcca gtctgcagaa aactcgagaa aagcttcatc
    41821 aataaccaga taagcatgac tttgggcaca tgcttcacta acagctagaa cttcagctcg
    41881 acttaggccg cagcccgtag ggttattggg attacacagc atcaccagcg caggacgctc
    41941 ttgtttgatt tgctcacata tttgttcaac ctgcagttta aattcatgct ttcgctgcag
    42001 ataatgataa ctgaccgctc tccctgcagc ctgagctgct ttggcataaa gattaaaact
    42061 cggtgccgga atcagggctg taccagctgg tagaatacgc attagcgtat aaattaattc
    42121 ccctgcaccg ttgccaacaa caatattctg tcgcggttga tagtactccc tgataacgtt
    42181 tactagttta cggcaagcag gatcaggata ggcagtaaca gtcgccagct gctctgccaa
    42241 aaatgctaac agtccggcag gcggacctaa gggattgatg tttgcactaa aatcaactat
    42301 ttccttgggg tcaaagccat aagcttctgc tgctgttttc acatcgccgc catgaccaaa
    42361 aggatgtagt ttagcttgtt gctgcattgg ttaaagcctc caatctttta attaaaactc
    42421 gatgccaggc tgtgctttta tccctgcctg gtagtgatgc ttcctcaagc tcacttcgct
    42481 gacaagatcg gcagcagcaa taatctcttg ccgagcatag cgcccagtca gtacaagatt
    42541 gacatgctga ggacgttcct gacacatcgt taagacgtca gtgagattaa gcaattcatt
    42601 gtgcagggca taattaatct catcgaaaat aaccagctca tagtcccctg agcgtaaggc
    42661 ctgacgtcct ttagccagca cagctttggc atcagccaca tctgccaggg gatcaaagcc
    42721 tcccttgcct ctaaaagtac tcctttttga ctgcactact gtgatattgg gcagatactt
    42781 gcggatagcc tgaatttctc catatgtggg gtcacttttg cgaaactgga ccataaatac
    42841 ctttttgccg tgtccactgg tacgtaaggc cagaccgagt gctgccgttg ttttcccttt
    42901 accgtttcct gtgtagacca tgattatgcc ttggttttcc tgcagtgata tcacctcctt
    42961 aacagcagct gtcaagctgc agccagaaat aacaagctca ggccaatcgg cctgatctaa
    43021 atgagcgtaa aaaagacacg tctcccttaa cccgagggca cgatatactg tttaacacag
    43081 gcaggtttcc tggctgccgg tcaacattgc aaagcgcctt ccccgacaag gcgagtggcc
    43141 tactgctttg caactcacgg ttacagtggc gggaccgctc aggctttgca cctgattccc
    43201 tttatactcg taagagtacc tgttttatcg catctgtagt tgtggattat agtcttacca
    43261 gcattaaaat tagtgcttta atgatcaaac cgatagctaa gaaaatcatg gcggtcagac
    43321 gcattagttt agtagtagta acaatatcat cgacattggc caagcgccct tcctgccata
    43381 gcaagggccg ctgggtgaag cctccatagt acttgttttc tccgcctaaa gtaacattta
    43441 gggcaccagc tgtggtggct tcggcaaagc cgctgttggg actgggatgc tgatgactgt
    43501 ctcgccgcat agcaataaca gcagctttaa catcaagccg cagtagtgcg gcagccagga
    43561 gcatcaccgg cacagtcaga cgggctggca aaaaattagc taaatcatcc agcttggctg
    43621 cggcacggcc aaaatataga taacgctcat ttttatgacc aagcatcgaa tccatggtat
    43681 tgaccgcttt ataaagtaag gctagcgacg ctcccccaag caaggcgtaa aaaagtggtg
    43741 ccgttacgcc atccactgtg ttttcggcaa ctgtttccac ggccgcccgg gccacttctg
    43801 acgctgttaa ttcgtcagta tcacggctca cgactaagcc cacagcataa cgcgccgtcg
    43861 ctaactcacc tgcacgtaag ggcttgataa ttttttcagc cgcttcctgc aggccgcgag
    43921 ctgccagggt agttgacagt aagacaataa ttacaagtaa ccccagatat ttattaaacc
    43981 gggtaaaagt aaacagtaag agcttgctgc caacataagt tgtccccagg actatcatca
    44041 ccagcacggc cccggttaca agctgggcag tgttcgaggc agtctcccga cgtaagcgtt
    44101 tttccaatgc tctgactaaa cgccccattt gaattacagg atggggcagc caggctggat
    44161 cacctatggc tgcatctaac aaaaaagcac tcaataattc cacttagcct gcctccttac
    44221 cacggtttga acagttagct tcctgctgcc tgcgctgcgc acagaccgta ataaaccgtt
    44281 gtgccagccg cggattagct aaaaaatgca aatgaagata tgaaccataa cagttatagc
    44341 ttctttcacc agtcagctgt tgcttttcag tctggtagag ataattagtc ccagcctgct
    44401 ccatctcaga ccaacgaaac tcatgtcccc ggcagctttc ctgcggcagc aagagagcat
    44461 tgtttgccgt acccctgact tctacataac caagggcgcg gcgaccttga tgcatacgag
    44521 agtgcccggg gaaaataccg cacatagggt aggtttgctc ctcaaaggtg gtcagggact
    44581 gagcaagata catataaccg ccgcattcag cataaatggg caaacccgca gctgctgctg
    44641 ttttcagctg ttctcgcaag gtaatatttg ctgacagttc tttggcatac agttcgggaa
    44701 aacctccgcc taagatagca ccatggcagt ctgttggcag ctgggtatcg ttaagtggtg
    44761 aaaaaggaat cagctctgct cccaatgcct gcaaatactc aaggttttcc tgatagtaaa
    44821 aatggaaggc ttcatcttgc gcaactgcaa tccggcacct gacaggaaca tgctgtgcag
    44881 gaaagacggt ctctgtaact tccggcagtt cacctgctgc agcagcagct gctaacaagg
    44941 agtctaaatc aaagtgtgtt tcggcaagat ctgctaagtt agtaaaaagg tcgggtaggt
    45001 tttctgcttc cgccgtaggc agcaaaccca aatgccgctc gggtataaca atcttagtgt
    45061 ccttgggcag ccagcctagg acaggcaggt tcaggtaatc tttgacggcc ttagtcagga
    45121 tttgataatg actgggactg cccacatggt taaaaatcac accggcaaag ttaacctcag
    45181 ggtcaaactg taaatagccg cccaccagtg ccgcggcact gcgtgccatt tttgccccac
    45241 tggccactaa aactaccggc gtgttggtga ttttcgccag ctgggcagag ctgccagact
    45301 ctccttcatt tttcctgcca tcgtataacc ccattacacc ttctatgatg gcaatggctg
    45361 atgaaggcac ccgtcgctgc agtatttccc tcacctgtgc tggccgcagc atccagctat
    45421 ccaggttgct agcggactga cctgctgcag cactatgaaa acccggatct atataatccg
    45481 gtcctacttt gaagggaatg acagtatgac ctcgtcgttt gagagcggca attatcgcga
    45541 ctgtaatcgt tgtcttacct acaccgctgt tggtacccgc tataagcaga cgtttcacac
    45601 tgctgcctct ttctgggtaa gagccaggcg taccagagca ttgacaatgg ctgcagctat
    45661 ggtgctgccg cctttttcac cccggatcgt aatccagggt atggcaaagg aagttaacaa
    45721 ctctttcgac tctgctgcgc cgacaaaacc aacgggagta ccaataatta atgatggttt
    45781 ggcctgtccg gcctgcacca cttccagcaa tttaaacaaa gccgttggcg cattgccgat
    45841 cacaaaaatt ccgcctgtcg ggcaaatatt taaagcatgc gtcatggcag ccactgcccg
    45901 tgttgtgcct aattctttgg cccctttggc gatcgcagca tcatgtataa ggcaggcagg
    45961 ctcaataccc agggtctgca aacctatcct gctgagacca gagcgcgcca tctccacatc
    46021 acaaaacaag ggttctcccg ccagcaaagc agcagtgcca gcatccaccg cctgttcacc
    46081 aaaagctatc aaagcggcaa aggtactatc accagtagta tgtactactc ttttcacaac
    46141 cggtttctgc agttcagtaa aggtaaaacc atgcaaacgt ccctcaataa tttccatgct
    46201 taaccgctct atctccagcg gatttttaat gtattccatc ttctcctcct ataccttggt
    46261 aaccgcggca atacggtccg caatgatctc tgccatccgc tcgtcgatgc ccagaatagg
    46321 tgcaagtgac aactccaact gcgggggtgt ttggccgttt ctgtcgctaa aatttccgga
    46381 aaatcttcct taatatgtac cccctgatac agaaaaacag gtacaataat aatctcttgt
    46441 atctcttctc ccactaactg cgctatcgct gttgacacat ctggtttcgc cagctgcaaa
    46501 aaactatagg ctaccacctg ttccggtcgc ggtcgctact tctgaacaag tggctgtagg
    46561 tcctgtaaag cccattagct tcactacgat ggctgcctgc ccaactaata atacagcaga
    46621 tttcatcttc cattctcctt atttcgatag accttttgca gcgcagcaac gatttccgca
    46681 ggggttgatt tttcctctat aggttccggc cgctgcacaa tgatgatggg gatattacaa
    46741 tcctgtgctg cagctacctt ttccggtgta ccaccagcag caccactttc tttactaatt
    46801 accacatccg ccccatattc tcggaagagt gccgcattga gctctgcaga agctggtcct
    46861 tgtagtgcta caatctgctt cggtaaaagg cccaaagaca gacagtgctg caagcttttt
    46921 tcaacgggca aaatacgggc cactacctca cacgcctcag gttttaagac ttctaaacac
    46981 tccgccaggt gtcgcgtacc aatggtcaga aacacaactc gacccttacg ggccgcctgg
    47041 cgagcggcag tgggaaagtc tggtgcaaaa tatacaccat ctgtactttc aagctcgcag
    47101 ccaggccgct catagcgcag gtaataaatc ccggcagcct gggccgccgc acaggcctga
    47161 gcggaagctg ctgcggcaaa aggatgtgtt gcatcaagca ggaccttgat ctgacgctct
    47221 ttgattaact gcagtagttg ttcgcaatcc agcgcctgaa ccaaaacctc acagccgcct
    47281 tgctgccgca aaagctctgc cccgtaagtt gaaacaactg tcgccacaat ctttgtggca
    47341 ggcaaagcct cccgaattat ggccaccatg cgtcgggcat cccgggttcc acccatcaaa
    47401 agaatcatac tgtataacca cgcggggtaa ccattttgcc atgtactacc ttagtctgac
    47461 tgttacctac aatcaccgta gtagtcatat caacttgctc tacagacaat tctgccaggg
    47521 tactaataac gctgctttct cctgaccggc caatattttt tacaaagcca accggcgtcg
    47581 acccgctgcg ataggtgagc ataatttcct gtgcctgacg caggtgttcc tgccggccac
    47641 ggctgcgtgg attatacagg acaatcacaa aatcagcact ggcggcggca gctagtcgtg
    47701 tttggatagt ttgccatggt gtcagtaagt ccgagagact aataaccgca aaatcatgta
    47761 ctagcggtgc acctaagaca gcggcagcag atgttgccgc tgtcaccccg ggaacaactg
    47821 tcacaggcaa tgtggccggc gccacctcca acaccagtcc tgccatgcca taaactcccg
    47881 ggtcaccact ggatataacc acgacatgtt ttccctgcaa ggcggtactc acagctgctt
    47941 tagctcgatc tatttccttt ttcatcccag atgacaatac tgtttgctct gttagcaagg
    48001 gctgaattag acgaatataa gtgctataac caaccaccac attggcttcc tgcagcgcct
    48061 tagcggcctg tggtgttaag agagcctcat tacccggtcc gaggccaacc acggagattc
    48121 gtcctctgcc acggcaatcg ttatttggtc taacttctgt ttgggcacca gcagcttgct
    48181 gctctgtgcc gcgagaattg ctgtcggtac gcatacactt cccaccccca ttatttcttc
    48241 aacaaatttt gatttttcat aatcatactg tacggcttga atttcagcag cattgaaaaa
    48301 gcgtattggt acctttaggt tataagcggc agtaagcaag cctgcttcat cccgtttttg
    48361 ctcaatactc gccagctggg caagtgaggc tgctgctaaa ttaaaatcct ttagtacagt
    48421 tttaacagct gcttctattc gttcagcagc aacaccacgg cggcagccaa caccaacata
    48481 tagattcttg ggtcgcagca gcagctcatt gacacacatc cctggtagct gctgacagtg
    48541 agtcagtacg gctcgcggct gcccctgagc tgttttaagt tgtgctaacg gttgaaaaga
    48601 aagtcctgca gcctcaccaa atacactgac tggccagtct gtaaacacag taaccggctc
    48661 accgcgcagc atggcggcat ttagctcttt cacccttgag acaggttcag gagttaagcc
    48721 aaactgatga gcaatcacgt ctactgcccg cagaccagca ccgtccgtag ccgtcgtaat
    48781 cacagctgtg ccctccagat actgcgcgag ctgctgtgcc agcgcattgg ctccacccaa
    48841 gtgaccggaa agcagcgaga ttgcaaaacg cccgtgttca tcgagcacaa ctactgccgg
    48901 atcctgctgc ttactctgta ggtatggtgc cagcagacgt acggcaatgc cgacagccat
    48961 aattaaaacc agcttccgct gttggcgaaa taaatctggc agcagtaccc gtaaagctgt
    49021 ataggggcat gcttgatcat ctggctgcag cagagaatga ggcaggtaga cactgtagtt
    49081 gaactgctgt tttaaacaca gagccagctc tctgccgcgt ggtgtcaggg ctacaactgc
    49141 gccactcatt ggtcctttcc tgcccgaaaa ccgtgtgaaa actccggggc atacaagcga
    49201 gaaggcttca cctgttcttc acgcaaaaac tccccgacca gaatcatagc agtcttggtc
    49261 acgccagctt gcttcacctg ctgggcaaga ttgtctaaag taccgcttac cacccgctct
    49321 tgcggccacg aagctttttc cactactgca gctggtgttt ccggcgggta agctgttaac
    49381 agttccgcca ctaccccctc aatcatcccc acactgagaa aaatgcagat cgaggcacgg
    49441 tgtgctgcca gcgcctgtaa attttccttt tccggaactg gcgttctccc cgccaggcgc
    49501 gtaataatca ccgtttgcga cacttcaggc actgtcaact cacgccctac agccgcggca
    49561 gccgcagcaa aagaggatac ccctgggact gtttgtgaat cgattcctgc tgcctgtaat
    49621 tgatccgcct gctcctgaat agcaccatac agggaaggat caccagtatg taaacgtacc
    49681 accttttgtc cttgctgcac agccatctta atcattgtta tgatttctgg cagcgataaa
    49741 cctgcactat ctaccagttg tgcctgtgga cctgcatgtt ccagaattgc tggattaacc
    49801 agagagccgg cgtaaataat caggtttgct tcccgcagca gccgcgctcc tttcagtgta
    49861 attaactccg gatcaccggg tccggctccg acaaagtaaa ctgtcatcca tttcttctcc
    49921 ttttagcgat tagtaaagaa agataatcta ctgcctggcc tcggtaggca aaggggtcat
    49981 ttgtccaaaa ttcatctttg ccgccaacac ggctgactag ataaacctcc tggttaaagc
    50041 cggttttctc caacagcgcc agcgtttcgt cataagcagc cgatactttg agtaaaacca
    50101 ctgtgtccac ataacgcgtc agttctacga attcttcctg gccaaccggc acaggaatca
    50161 cggccattct ttcatcattt tcaagcaaag gcacttgcag cagggcggca gctgcattga
    50221 ttgaagagat gcccgggata atttcagttt taaccgtagg tgtcaatttt ctcagaaatt
    50281 gtaataggta agaaaaagta ctgtacagca atggatcacc aattgtcaga aaagaaacca
    50341 ctgagtgttc cgttagtagt tgcgctaccc gagatgctgc ctcccgccaa tgctgtgcta
    50401 aaaccactgt gtctcgtgtc atgggaaaca gcaattcttc tattaacacc tccgctgcta
    50461 aatatggttg tgcagtttgc agagctacac tctcccgctc cgctttagat ttaggaacac
    50521 aaacaacttg cgaagcagcc agagcgtcaa tcgccgtttt cgtcagataa ccactatcgc
    50581 ccgggccgac gccgattccg tagaaaatct cctttgtcac tttgtttcct ccttttgggc
    50641 actgataatc caaactccgt tttgcgcctg gaaataatct tctcgaccta atttttgcag
    50701 gcgtgatgcc tgtacataca agccatctcg gttggtaaaa ggttctgccg ataacctttc
    50761 taaagtacgt accatgttct ggggcgttac tacatttacg accagaatcc catctggcgt
    50821 taacttctcc ccgcaataag tcagaatttc tgtcagctgc ccgccactgc caccaacaat
    50881 aacccgctgg ggctgcggca atgacaccag cgctgccggc gcttctgctg ccacaaccgt
    50941 aagattatct gtaccaaaac gactgctgtt ttgctgcaat agttctacgc cttccgcatt
    51001 gcgctcgatg gcataaacct ggcttgacgg cacagctaag gcagcttcaa tagctaacga
    51061 acccgtgcct gccccaacat cccacacaac ctgtcccggc tgtaggcgta gttgagctag
    51121 cgttaacaca cgcacctctc gtttggtcat gggcacccgg ccacggataa aaactgtatc
    51181 aggcaggcct aaaccgcagt aagtccagtc gttaggcatc ctcaatcacc atcacacagt
    51241 cacctgccgt tgctgcatca agcaaactta gacgagtacg ttgaatctct tctgccggag
    51301 ttgtcagtcg ataacataga gccacgccgg tatcgctgcc gcctgcccct aaaatcattt
    51361 tagcaatcct ccctggcgtc cactgctgat cggtcaaaat ggctttcttc cccgggcgca
    51421 gcaagcttgg caataattcc tctccattgc gtccgtgggc agataaaagt gtagcgtcct
    51481 gccaaggttc agcaaggcgg gcaaacgcca cctgcattga tgaaacacct ggcaatactt
    51541 ctaattctgc tgccgtaaaa tgccggcgta aataagccag taaactataa aagccgggat
    51601 caccagaaac cagaaccaca atttttttta aggctgccag tttacgtaaa cgctctgcta
    51661 aaccttccag tttacctgtc aagggaatga tttctttctg cagtgaaaga aacggtgcag
    51721 ttgctctcgc cccacctaac agcacatccg ccgcctctac ctgctccctg actacaggca
    51781 atacatattt ttcaccaccg ggtcccactc cggccagaca aattttaagc attatcttcc
    51841 acctccaacc attttgctgc ctgcagatca tgcgccacta tatccccggc caaatttaga
    51901 aaaatgcagc ccacctttac accgccgcca gctatttttt ctgctcttct tgccgcagcc
    51961 tgtgcgcact gcctcagaac ctgctgataa ccatgctgcc gtaaataagc agctgattgt
    52021 tctgctgtgt tatgtgacat taaatcctgc agcactgaga gcggtaaccc tgctaaggcg
    52081 gcatgggcaa ccagtgtttc tcgccgggca tccgctacgg cactatgagt ttctgctatc
    52141 ccggccgcca gtttaactag cttaccaaga tgcccataca gtaaaacttc cttaaccctt
    52201 ttggcagcac aagcttttaa catatagcca ataaagttgc tggtgacaac cacggcatct
    52261 gccgctacac ctaaattttc tatggcattg cgcttcccca ttttaccggg ggttagcacc
    52321 aaacgctgct gcccggccgc cagtgccaga tcgatttgtg gcaccagtga acgcttaaag
    52381 gcagcggacg acatgggctc cacaatccct gtcgtaccca aaatggaaat tccccccacg
    52441 atacccagct ccgggttcag agtgcgtttg gccagctgca cacctgcagg gactgagatg
    52501 gtaatggccg ctcccgcctg cgcaggcaga acgctggcca cagcctggcg aatttgggcg
    52561 cggggaacag ggttaatggc aggctcaccc gggggaatgg ctaaacctgg ttttgtcacc
    52621 agacctaccc cctccccagc ctccaactta aactcccctc gtggcagtgg ggttgcttgc
    52681 gcaaaaattt ccacctgatg tgtgacatcg ggatcatcgc cggcatcttt gctgataccg
    52741 acacgcacgc cggcagtttc ccttatcact gaagaaagtg gcaaagtaag ttgaatacca
    52801 gccggtgtat ccacagtgac ttctgtgatt gtttgctccg agtacaaaag tattacggca
    52861 gctttggcag ccgcagccgc acaggtgcct gtggtaaaac cagctcgtaa aactgtaccg
    52921 tcagcttgtt gaaaggttgg tagcatcact catgttctcc ttttactgct ttttaatcca
    52981 tctactaact tttattgtat ttattaactc ttgttagaac aaataatctc agaccgacgg
    53041 tctgagatta tacgcataaa aaaacaacgc ttcccttaac ccgagggcac gatagctttg
    53101 gagtaataca ggcaggtttc ctggctcacc ggtcatagtt tcaaagcgcc ttccccgact
    53161 agcgagtggc atactgcttt gaaactcacg gttacagtgg cgggaccgct cagggtttac
    53221 acctgattcc ctttatactc ataagagtac ctgtttccac acatatttaa ttagctatag
    53281 cttaccagat agttcatact tttgcaacat cttttttcga gtactgaaca tctctttgcc
    53341 gccgtacttc acgattctac ctgtggttga gtaaatctaa tacaaagcgg ttgctgattt
    53401 atcttccata tgcaatacta aagcaggaga aggagatgaa tcaagatgac aaattacgct
    53461 tgcggacgct taattgcaga actacgcaaa gcaaagaata tgacccagaa ggaactggcg
    53521 gcatatctta atgtatctga taaagcagta tcaaaatggg aacgtggcga gaactatcct
    53581 gaagtctcca ttttgcccca actggcacat aagcttgaaa ttactgtgga tgagctttta
    53641 aatggggaaa ttgctccgca gactgcaagc tcaactaacc cgcctgaggc tgaggatgtt
    53701 tatgcatatt cctgcttatt agaagatgcc cacttaagat acaagaagca caccctgaca
    53761 gcttatgcca tcttaactac cggtatcctt accggctcca tcattttaaa caatatcctc
    53821 ccccaggttg ctgcagtggc ctttgctttg accggaacac tcttttacta ttttgctcag
    53881 agccaatatc agaatgccag ggacagattt gcacagtttg caccagcagc tgttactgca
    53941 gacggcacag ctgtcactct gataaaatac ggcagtactt ttatcggtat agctgtttcc
    54001 ctgctgctca tcctaaatac catagctgca agcacccctc ataatttatt tattcagaac
    54061 aactatttgt tgatagaaaa aactgtaccg ttcccggccc ttttgctgct tatgatcatc
    54121 aatcttctgc tgtatgttta tcaatataat ggctacagaa gggatcgctt gctaaagatc
    54181 tccagaggtg attttgtcag aatactaagc agcttcggcc tatcaatcat ccttgccgca
    54241 cttctgctaa tccttcagct ccagatgatt atccgtgagg gagcatataa tgcaatcttg
    54301 atcaagaatg taacattact ggccttagtc ttcgctgtgt taagcaattt ttgtttactg
    54361 ttctttaata aacgccacag ctttaaaaag ttcctgcaca tttttgtaat atctatacta
    54421 caagccggtt ttctcatctt tgcagccaat caatacgtcg tctccaccaa gcattatgac
    54481 tccaatgttc ttggcctcgt ctacactggt aatatcatta cagtggtgat gctggcgata
    54541 acaattttcc actttaccta tttacaatta tctcgaaaaa agtacgctta aagctttagt
    54601 ctcgcagctg ccagcaaacc ctgcaccaca gaagaagcca gcaagccgca gccctcatta
    54661 gtggggctgc ggctttgtct gttgcattca tgggcagggg attgttttaa agtgcggcac
    54721 cagttttttt aagtcagcca gtctattgcg gacaaggcga gtccccagct gctgtgctgc
    54781 gctgtgccag cctattcgct gcaaaaaaca acaccagcct gccacttgag gattttctaa
    54841 gacacaagtt atgataacta gttcacttag cggtacttgg taaatcgaca ataagctgtg
    54901 tataccgcgg acaaatattt cgggatactt tttaacagta gtgaggtaaa tttgctcacc
    54961 atcttcagtt agaccaacat agcgaattac tccctcatct gccttggtaa cagcacgaaa
    55021 atatggcagt tcacacaatt tcagtggaga agggggagca tcccagttaa gctgttttag
    55081 atgaagggaa gccgcagccg caggcaaata ataatcggtt aaccctaggt aaagataatt
    55141 catcctgcta gcttgacctt tacttgtgct accagttcaa caattttggg atatgcctct
    55201 ctggtgccct gaattaccag cggccggcct actgaaataa tactccagcg gcgagacaaa
    55261 taaccaccaa tgcgcatttt cagggaaacg cagtgtaaag tatcgacaaa aacaagttgc
    55321 tcgtggatac caagcaattt tgacacccca tgtaaaatgt ttaccattgt ctgtcccaca
    55381 ttgcggcacc ctaccgaata aatttgattg ccccactcat caaaaccaaa gaaatgtaac
    55441 tgcccgtggc cctcggcagt ttgacggtca aatagtgcta aactaagcag ctcttgtgcc
    55501 gtaggaattt tatctgcttg caattgccct aaatggatgg cagcggcggt aacggatgaa
    55561 tgcgcaccac cgtaacaatg atagattatt ttttttaccg tcatcctatc ctccagtgtt
    55621 atgttaatgt tttacaataa tatagacacc attccccata tcatgggcca ctgcttccat
    55681 cacacgcgag aggatggtac acatgttaat catttcctcg tctgtctcgg catagaaaat
    55741 agggatccca gccacacgat gttcgccttt tctagtggtc acagcagcaa gaattatctt
    55801 ggcaatggta acttccatct catcctccta atctgccgca ctacggccaa tgcgggactt
    55861 taatggctgc acaaaagcgc tttccagcac cgggacattt tgcacagcag ccactaaaca
    55921 ttctatatcc ggctcgatag gtacaataac catgcctact ctgccggttt caggcttccg
    55981 acgcagcagc ggggtaaatt cagcggtatc tacatcacgc cgcaccccta acaagagagc
    56041 gacatcatgg gcaattgctt gtctttgtcc cacatttgct aatatttcac ggccattatc
    56101 actaatcggc tctaagacaa caccgcgagc catttcttca taaacttttt tcacttcagg
    56161 caaggctaga ttcatgatat gtatatcttc aacaaagaga ttagcaccat caaagtgcac
    56221 ttttgccggc cttactttgg caatatcacc aattaatttg ccgcgcataa aacggcggac
    56281 agcagtaagc gcaatggcac cggccacaat accaggcagg ggtgaataaa gataggtaaa
    56341 aagcgatgtg tttagtgaga caaaaacgac caaataattg cgtgcttcaa aagttcgggc
    56401 gatgccttca ataaaatctg cacctcgcga cactaaagtt gattgatcta gggagaccag
    56461 catttcccgt tccatattgc gcacatcgcg gaactgtgta gctgccaagg ttaaaaaggt
    56521 gactgctgta tattcctttg ccgcaatggc agggatggcg actgccccca gggtagccgc
    56581 cacaaatccc aatgccagat gagttacagt tgcatgcgga taagaagggt actgacgaaa
    56641 atcacggcgc atcatccaat agcgtgctat cgtacccatg gttacgccgc agataatcgt
    56701 cagaatatct ttattcaagc tcttcagctc ccttccgatc ctccatcgga actatcatct
    56761 tcatcttgcc tcgatcccgt gagatcggcc atttctctgc ttctgaactg atttaatttt
    56821 gctttggctt gctcaaagcc accagtgctg atcaagagaa atatcgccac agcagcaacc
    56881 agaccagcaa taatccacaa aacagaacgt agggccgtgc cgccggcacc ctgcgaagtc
    56941 gtaggactgg gactggcggc ctgttcacct tcggctgtgc catagataaa atcattcatt
    57001 acctctgaaa agaggcccat ggtctcttca gcttgatctt tatcattagt atgggaacca
    57061 acctcaatca aagtagcacg aggtgacatg tcctggttat agttgcctcg ggccataaag
    57121 atccctttta ctaaaccggg atatttttcg tcagcctgct tcttaatacc ctcggcaaac
    57181 tgctggttat tagcacggtt ttgattctgc ctgcccacaa ccagctgcac ttgtactcgc
    57241 ttttcacctt ctacttcttc caaatactct tcttcgggca ctgcatcacg gtgcacatca
    57301 atcagcgcat ccggttgttc cttgagagct tcctctactg tacgtcgaga gcgttgataa
    57361 gcgccagcgt catggggtgt atggggttcc ttggaacgga cgaccttaat gcctttttct
    57421 tcaaacttct tttgcagagt gtccgctaca tcaagaattc ccccaccggg gtctttactc
    57481 tctgtaccat caccatcaat gtacgattca gcgccatgcg agctgtagat ggcaattttt
    57541 ttggttttct gctctccttt tttctgtaca ggtactgcag aacgtaacat attacttact
    57601 cgtgccagca gaccaggttg tggtggtgct gccaaggttc ttttttcaat taactccgcc
    57661 cgggccgttt caccgcgaac cgaagtgacg cgatagtaat tattatcagc actaatgtat
    57721 tcatcaccaa ccacaatttc tctggctgta cgcataatga ccttgccgtt attatcttcc
    57781 atggtatagt aaacgccaga acgatgatca ccatcaatac catcaatcag gttatcgatg
    57841 gcaaaactgc tttgtgtaga aaagaaaagc aaactaacca ccattactaa aactgctgtc
    57901 agcttaaaag tgttaaagct actcctcctc atgattgttt tcctccttag tcaagctatt
    57961 ttcatcatga gacaactctt cgctaaattc tccttctgac agacctttcc tcagacttga
    58021 cggacggtct tcggcaggac ctccttgcaa acgttctcta gtttcaccaa aaatttctgc
    58081 cagtaaaagc gataagattc ctgcaatcac cacgccgtca aagacaccgg ctccgccgat
    58141 taccatgctg ccagaaccac cacgcaaggc aacttcaatc cgtgaataga tatctgtcag
    58201 caataacccc atagaaccgg caataaaggc agaacggcgc gagcgcccag ctaaataacc
    58261 aacaacagct gcaataatgg aaaaaatgta gaatggatcc aggaacctgt tataggtagg
    58321 ctctgtgggc aaaattttca ttgctgcgta aacagcaaac gaggccacca gagcagctac
    58381 tactgctctg attttttcct tggctgttcc tgccttgata atcagataaa cagctaaagc
    58441 taaaggaatt agagcaccac caatattaat ggacacatta tcactaaggg gaatatccgg
    58501 caaaaagccc cccactacca tcgcgccgac aaaaagcagc gccgcgcggt ctgttaaccg
    58561 cagtctgtcc aagacgcgct gggccgcacc tagataaata gcaatcgcca cggccaccag
    58621 cactaacatc ccaattgtca taatcctaca cctccagaaa tcaacagttc ttaacatagc
    58681 gttcgcagaa aacattagat ttatgcgaat tctgcgtgtc aggtttgaat attttaccta
    58741 gagacttcgc gttgtttgcc aaggttggtc taatttcctc tagattaaaa aagatgacag
    58801 ggtgtaaatc cctgtcatct tgcgtaggtt acttatttac tttagcggct taagccgcgg
    58861 gctgtcatcc attctctagc ttggccggtg attaccagcg gcacttcttg taattcacga
    58921 atgctggagc agcctgtcat caacatgacc atttttaatt ccgttaggat tttctgcaaa
    58981 cttgcgacca acttttccgg ccctgctttc tgcaagatcc ttaaggctaa accggcgctg
    59041 cctactgctt gtgctcccaa agccaaagct ttggcggcaa gcaggccgga attaatcccg
    59101 ccggaagcaa taatatctac agcaggagca cccgcttttg cttccaaaat ggtcagaggt
    59161 gtgcgaattc cccactgcag cagctccgcg cttatttgct gtttagtgcg ggcgctttca
    59221 atctgcagaa agtttgtccc gccttggcca ccaacatcaa tggcggctac accaatatcc
    59281 tgcaattgcg cagcttgttc cttggcgatg ccaaaaccaa cttccttcac aattaccggc
    59341 acactcacag ctttaacaat ggcggcaagg cgttgacgcc aaccgcggaa atctctgtca
    59401 ccctctgcca tcattagctc ctggggaaca ttcaaatgga tttgcagggc atcggcagct
    59461 accatctcca cagcttgctg tgcagctgtg agcccagcat tggcaccaat gttggcgaaa
    59521 ataatgcctt gagggtaaac ctcacggacc acacgatagg tttgctggta gcgttgattc
    59581 tctaacgccg ccatttgtga gccaacagcc atgggcaagc cacagctgcg agcagcaagg
    59641 gctaaatcat ggttaattgc ctctgcagcc tcgactcctc cagtaatggc gttaagcaac
    59701 agaggttgag ctagtgtcag ccctgcgact gttgttgtta agtccagcgc ttgtagagca
    59761 gtctccggca ggcagttatg aattagctct atatcactaa aatcagcact tgccaaatca
    59821 agctgaacag catagtgcaa atgttccagc tttcttacct gccgagtcat ttactactta
    59881 tccttattca tgccatcaaa caggtcacca aagacatcac ccagggttag gcccgtacct
    59941 gaatcctgat gctgctgcgg tgcacgctgc tgtggttcac gtttgggacg tggtgcagct
    60001 tcgcgcatgc tcaaactgac tcttttagcg tcagtattaa tgtcaaggat cttaacttta
    60061 accacttcac ctttttgtat cacatcagaa ggctgcttga catgatagtt agccagttgc
    60121 gagatatgca ctaaaccttc tactccaggg atcaattcca caaaagcacc aaagtcaacg
    60181 aggcgggtaa ctttaccttc caccacatct ccaggttggt actgtttatc aatctgtgtc
    60241 cagggatcag gttgagcttt acgtaaagaa agaccaatcc gttcccgttc tgggattact
    60301 tcggttactt tcacatcgat ttcttccccc acacttaata cttcaccagg ggtttcaatg
    60361 cgatgccaag aaatttcgga aatgtgtact aacccatcaa tgccgcccac atcaacgaag
    60421 gcaccaaagt tggtcagtct ttttactgta ccgtgaataa tttgacctgg ttgcagggag
    60481 tttaggattt cttccttctt aacttgggtt tcttcttcca gaacttgctt ccgtgacagg
    60541 atcagtttct ctttttcctt gcgcatctcg ataattttaa aagatacttc ttggtttaga
    60601 aacactgtaa agtctgggac atagcgcatg tcaaccaagg aaccaggcat aaaaccgtca
    60661 taaccgccgc ctaagtcaac aaccatcccg gctttaactg tttctttaac agtacctgtc
    60721 aagacagtgc cctgattaaa tgcttcctct aattcttccc atctcttttg acggtcgatg
    60781 gctttacggg aaagcgttac tttgccttct tggtcatcca tgtcttggat tacaacttca
    60841 atttcttgtc ctgtttgcaa aaccgcagag agagtttggt cagcttgtaa tataacttcc
    60901 tggcgaggaa gtaccccctc gctcttgtac ccaatgtcaa ccagggcctc gtcatcagac
    60961 acctgttcaa cagtgccttg cactacatca cccacggaga aatcacgcat tacctgatcc
    61021 atgggcacct cctcaacttc cacctgctct tcttgtactt ctgcttctag attcttttca
    61081 ttttccatct taccaataac ctcctttatc aaccagtcgg gtgttgaagc accggcagtc
    61141 accccgactg tcctcaaccc ttgcaattgc gcggtatcta ggctgtccgc atccgtcact
    61201 tcaatggttt tagtgcccgc agcccggcag attgctgcca gtttctgcgt gttgctgctt
    61261 tttttactgc cgattaccac cattgcctca gctttttgag ctaattcgct ggccgccttc
    61321 tgccgcaatg ccgtcgcgtt acaaatggta ttatatatat gaaccaccgg tactaactct
    61381 tttagcttct ccacaatttc ttccagaata cgaacttgtt gtgtagtttg tgccactact
    61441 gccacaggac gtgtggaatc caaacctggc agctgctcaa cttctgcggc tgtctgcacg
    61501 acaaaaggcc gttcatgccc ccagcccaaa actccttgta cttctggatg ctcgcgggtg
    61561 ccaactatta caacttgctg ccccgcctct actaaagccg ccacctgctg ctgcagcttt
    61621 tgtacaaaag tgcaagtagc attgataatg tttaatcctc gtgctgctgc tgcctgtaag
    61681 acttctggtc gaacaccatg ggtacgaatg ataactgtag caccactggg cacgcttgtc
    61741 aaatcatcta cctgtctcac accctgctcc gctaaacgat caattaaact ttcattatga
    61801 gccagcggac ccaaggtata gattggacct tgtgcggccg ttgccagcgc caaagctact
    61861 gcttttttga cgccgccgca aaaaccggcc ttctctgcca taataatctc cagcgtttct
    61921 tcacctcaat ctaactttta cgcagacggt ctatttcttg catgatccgc tgcgcgatct
    61981 tagcaagctc ttcacctttt accttgaccc cataatattc aggaaagtaa aggggcgtgc
    62041 caatccgtac acgtatggga tgaaacaagc gataaggtcc agcaatacca atgggaatta
    62101 caggcgcttc acttttggct gcgattagac cgacaccggc ttgtggtgtt aataattcat
    62161 tagtatgact gcgagtacct tctggaaaaa tgcccaaacc gtgccccgct tttaaggttt
    62221 ccagggcagt tttgatggct cgccgatcag cactattgcg tttaacagga aaaactttta
    62281 ttgcttttag cactgtaccc aagataggcg tctttatcaa ttcttctttg gccatgaaac
    62341 gggcaaaacg cggtgtgatg gcgccaagca gcggcggatc ccaccaagaa aaatgattgc
    62401 cgcacataat aaaggggcct tcttgcggta tattctcccg tccctgtaca tcccagcgaa
    62461 aaaagattgc gaatattact gcgaaaaatc ttctaaagat ttggtaagcc atgcccttcc
    62521 ccccgctatc acctggtaac tgtttctaca attaaagcta ctacctcatc tatcgttaac
    62581 tctgtggaat caatatggat ggcatcatct gctggtcgca gcggcgaaca ttcccgctct
    62641 gaatcaattt tatcgcgggc cgcaatttct tccttcatct gctccagcgt cagtgactga
    62701 cccttttcca gcagttccgc caaacgacgt cgagcccgtt cagctaaaga agcggtcagg
    62761 tagaatttat actctgcctc agtcatcacg ttagtgccaa tatcacgacc atccattact
    62821 acgccgccac gctgacctat ttctttttgt ttttgcacca tcacagcacg tactcccggg
    62881 cagcgagcaa ccagcgacac atgacgactg acctgcggtt cgcgaatggt ctcacttaca
    62941 ttttctccat ctaaaaaaag gatattttgt ccttcgtcat tatattgtac atccaaatca
    63001 gcctgagcag ctaaaactgc cagcgcttgc tgatcctgga agtcaatctt ccggcgcaaa
    63061 ccggccactg tgatggcacg atacatagcg cctgtatcta aataagcaag tcctaaccgt
    63121 ttggctactg ctcgggctac ggtactttta cctgcaccag ctggaccgtc aatggcgatt
    63181 ctcggtttca tgcccaaacc ctctttcctt atgccgccaa gctatttttt cgcggaaagc
    63241 gttttcttgg cctaaaatag cacaacaaaa acaacacact aggtgttgtc agaaacgagg
    63301 tctggccgca gttggcgggc ttccctcaaa tagataaatt gcacagcttg gttttgctcc
    63361 atgttgacat gcatcaagac ccggatacat ctgggtaaag cgccgggcac cgccatttct
    63421 actgtatcaa aaagagctgc agctgtccag ccaagctcac gtgcagcggc agcaggaaaa
    63481 gcagcgttta gatccggtgt tgcggaaaaa atgatactaa caagtgcagc tgggtcaatt
    63541 tgattaacct cagccatttt atgtagtaat tctcttgtgg cagctttaat ttcactagca
    63601 ctgttttgtg aaactgtagt tgccccccga atacccctca caacacaaca acccactacg
    63661 acgtcctcct acttacccgc tatatctaaa ataatagctt agattaaacg atttggcaag
    63721 ccttttcccg ctggcttaat caactttccc cactgagaca aaggaaacag tctccggctg
    63781 accaaaaaaa gtaatctgct tcccctcaat tggcgcaata attccctctg aaaccgaagt
    63841 cacggcgatc accacatctt ctgttggcac tttactatca acttctacct gatccccgtc
    63901 atgcaccgct actaataccg agctgccgtc ggcaaaagct gttacagtct cgccattcac
    63961 caacactttt attcctgtcc catccgcatc gttaataatg cttaatctta gatatcgctg
    64021 ctcattatca acaacaggac gtgaaattgc tggctgatct ggctcctgct gcgttaccgg
    64081 cgttccctca aaacgatcaa ccaaacttaa aatactgcgg aaatctggcc gcgtcagcaa
    64141 tgcttgggtc agcagcaaca aaaagagcat cacgccaaca cacttaagca taaaacgttc
    64201 aaagcgactg gtacgttcct ggaaccaacg cccaaaatcc atcccctagc cccccttcta
    64261 ccaaagagta tgccaaagag tagggaattt atgcctgttg ggctgcagaa agaccggcta
    64321 caaaaccagt agcaaaggct gcttgtaaat tatagccccc cgtattgcca tcaatatcta
    64381 taacttcacc ggcaaagtag agtcccccga ccagcttgga cgccatcgtc gtcggctgaa
    64441 tttctttgac tgcaacacca ccagccgtaa caatagcctc acgcaatgaa cgcatgcttt
    64501 tgatatttag ggttaagtcg gtaagtgcgg tgagtaaagc ctcgcgctgc gctttagtta
    64561 tttgattgac cggcaattcc ggatcgatgc ctgaaagcat aataattgtt tcaatcaggc
    64621 gctgcggcaa gagatcatta aggctgtttt taaactgttt acgaatatta ttttcgaaat
    64681 cacgcagcag tcgagcatct aacttctctc gcgttaaggc tggttttaaa ttaagatgca
    64741 gttgaggttt ttgtttttta accaaggcgt taacagcttc gcggctgata gtgagaataa
    64801 tggggccgct gacaccatag tgagtaaata acatttcacc gaattcttcc gccacgacac
    64861 gccctttaac tgttaattta gcagttacat ttttcaaagc cacccctgtc aactcacgca
    64921 cccagttttc ctctgtttct aaaggcacta gggctggccg ggttggcaca atggtatgac
    64981 caaccgcttg ggccagacga taaccatcgc cggtagaacc agtggcagga taagaagcac
    65041 ccccggcagc aataattact gcatcggcgg caatctcttc ttcgtcacaa actatccccc
    65101 gtatttcccc tccctccgta atgattagct tggtaacctg tttacccagc tgaacttgca
    65161 ccccttggtt ttgcagccac tgcgtcagag catccacgac atcttgtgcc ctttgtgact
    65221 cggggaaaac ccggcctccc cgttctacta ctgtgggcac ccctaagtct aacagaaact
    65281 gccgcagctg atcattcgta aagcggtaca aggggccgta aagaaaagtt ccgttaccag
    65341 gatactgttg aatcatctct tcaatctcag ccgcattagt aacattaccg cgacctttgc
    65401 ccgtaatggc caacttcttg cctaacctta aatttttttc cactaagatc acatttgccc
    65461 ctgcttttgc tgcctgcccc gccgccatca tgccggctgg ccctccgcca attactacta
    65521 tcttcatcta tatcccccta cacaagacct taatatccat atcataacca aaagcactta
    65581 ctcaaaaaca ttaccaacct tgtaggaaac tcgctttgca gacgaaaaca gaaaagcggc
    65641 ccgcgccgct tattcctctt tccatctatt atttaatccg cataacgttt cattttttgc
    65701 cgccactgat tttttgcttg gctgtgcact tcgaagaata tttgttccat ctccggcacc
    65761 gacatttgca acccgctggg ctcaacaatc tttaagcggc gaaattcatc ccgaactgca
    65821 aaacgaatga gaaaattaaa ggagtcagct gtcacttcca gctccaaagg tgcaaaagcc
    65881 acttcatcat ctacctcttc atcataaaca ctatagattt cgcccagctc aatattctcc
    65941 acaaacacac cttgtactgg tacattacgc cacaaagcag cctgttgttc ccgcagttct
    66001 gcagctactt cctcggttcc cttgccgcca aagaagaaac gggaaggacg ggctttacca
    66061 cgataatcaa aacggatgcg agcgcggatt ttttcagcgt ttttcatttc agttacctct
    66121 tcagggcgcg gctctgtaag gggaagttgt cgttcaaaac ctggttttaa cacattgctc
    66181 tcctccaata tggcaccttt ttgttataat ttcccgtttg ttggaaacta attcgtgtta
    66241 actaacagaa atcctgctat ttttaccatt ttcccctgat aaattttgcg acaactgacc
    66301 ttccagccag tcaataaatt gtcgtgctgt gcgtggtgaa cggccattct gccacctttc
    66361 ccagcgcagg gcctcactgc gtaagtgtga ggtctcaacc tgcagctgcc gctgctgtgc
    66421 caagccttcc acgatctcca aatacttacg ctgatccggc gacaaaaagg tgatagtaat
    66481 cccgaagcga tcagccaggg aaagtttttc ctgcatagta tcagctgccc gtacttgatc
    66541 atggagatca tccgactgca gccctgcccg ctcactaaac ttttctttaa ttaaatgacg
    66601 tcggttagaa gtagcataaa taaccacatt ttgcggtctg ctctccaaac cgccttccaa
    66661 aacggcttta agtgccgtat aacgttcttc attatcagca aaagctaaat catcaacaaa
    66721 aatgataaac ttctgttttc tgcctttaac ttcccgcaag actaagggta aatccgccag
    66781 ccttgtctgc ggtagttcta tcatccgtaa accctgctca ccatactcat taaggagtgc
    66841 tttgacggtc gaggacttac ccgtgccccg atcaccataa aggagaaggt tattagctgg
    66901 ataaccccgc agtagcatta atgtattgtc tatcacctgc tgacgttctg tttgataacc
    66961 tatgagttca gcaaaggaga tcgggtcaac tgcagtgatc ccttgcaaac aattgctttc
    67021 ccagcgaaaa ccaatatagc gggcaaagtc accggaacca tgctgctgat aaaaaaccgt
    67081 taaatcaggc agacaatcaa cccactggtc agctgtttgg aacttctgca tcaattcttg
    67141 ggcagcgctg tgcctgctca cagccggact gctagtgtcc cattccggca gctcctgtaa
    67201 agcagtagtc aggtagcttg agtttgatgt ccgctctaaa gctgctgcct tcagcccgct
    67261 ggctgtcagg gccgcaattt cctccagcct gctcacatca tgacgcatcg cctctactat
    67321 ccggggggtg agctcatcgt aagcctggtg ctcacacctt tgagaaaaaa ctgtatcgca
    67381 aaaaagtagt ttttccaaga tatattcctt taaggatggc actttatttg cttgcagcag
    67441 cgtgtaataa aaatcggcat aattatttag ccaaacataa ccatcaaaag aagtagtcct
    67501 taaactttgt aacaaacgct gatatttagc aatcaccgga tcagtaagat agccgcgcag
    67561 gacggttaac gaatccaaag caatccgagc cctaatgata ttattcccca taagtttctc
    67621 cccttaatgc agaaatttta caacggtctt taagtaatgc ccctattata tcatatgtgt
    67681 gggaaatagt agaagcaaag gaacttctcc tcgcccacca agtgcataat tcatgcctga
    67741 ccccacaata ctagccatag cgatgtaaga aggaggaaat aaagttatgt atgaaatgtt
    67801 tctctcgccg ggtaagattg gcaaactaac actaaaaaat cgcgctattt ttccgcccat
    67861 gggtactggg tatgtcgagc atgaagaacc gaaagagcaa ttaatcgatt atcatgtgcg
    67921 tcgagtgctg ggcggctgtg ccatgaatat cgtagaaatt actgcggtgc atgaaactag
    67981 caaatcaccc accaatcttg ccatttatga cgataagttt attcccggac tagctcgttt
    68041 agcggcagcg attaaagaag ctggcggcat tgcttgcctg cagttatggc acggcggccg
    68101 gcaaacttca ggtaaaccct ttgggggaca accagtagcc ccttctgccg tgaagaatga
    68161 ctttattaat gaggagccac gggctttaac gctagaagaa gtaaaggaac taataaattg
    68221 ttatggtgaa gcagcacgta gggcacaaga agccggctat gaagccgtag aaatccatgg
    68281 ggcacatggt tatttgatcg accagttcct gaacccctac accaataagc gcgacgatga
    68341 atatggcggc agtctggaaa accgagcgcg ctttggtgta gaggtcataa aagctgtacg
    68401 tgccaaagta gggcaagact tccccgtgct ctttcgctta agtgcccagg aaaatgtgcc
    68461 tggcggcatt aagttagaag aaggcattgc cgcagccaag ctctacgagc aggccggcct
    68521 agatgctttg gatgtctccc aaggctgcta tgacgccctg ccttacactg tgccccccta
    68581 ctttttgccg caaaaagtaa atgctgctaa tgcggcgcag attaagcagc agactaaggt
    68641 accgattatt gtcgccgggc gcattaatca tcccgagctg gcagaagaaa tattacagtc
    68701 tggcatggca gatttcattt ccttgggccg cccacagcta gctgaccccg actttgtgaa
    68761 caagacggcc cacgatcacg ctgacgagat tgtccgctgt attgcctgca atcaaggttg
    68821 tgtcggcaga ctatttaaag gtttaggcac cagctgtatc tttaacccgg caaccggcca
    68881 tgaacgagaa gtagttattc aaccggcccc aactaaaaag aaagtgctga tcatcggtgg
    68941 cggaccggct ggtttggaag cagccagagt cgctcaagag cgcggccacg atgttgtctt
    69001 gtttgaaaaa gatgtcggcc tgggcggaca acttgtcgtg gctgggcggg caccacataa
    69061 agaaatcttt gcagagtctg ctattcatct gggttatcgt gcttttaaag ctggtgcaga
    69121 tatccggacc tatacagaag ctaccgaaga gagaattcgc aatgtgcagc ctgatgtaat
    69181 catcatcgcc agcggtgctg accccgcact gcccaaaatt cccggcggcg accaacccca
    69241 tgtttacgaa gcccgtgctg tccttaatgc cgaccaatta attaaagcaa atcaagtagt
    69301 tgtcgtcggt ggcggtctgg tagggctgga agtaatggaa gtgctgctgg cgcaaggaaa
    69361 acatgtcacg gtgattaatc gcggcgctga agtgggtaaa gaattggaga cctatattaa
    69421 gccctatgtc tttggcatta ttgaacagga gcaggtgcct atcctcacca attcttcctg
    69481 cgtggagatt ggtgatggtt ttgtcgtcgt ggagaaagat ggccaacagg aaaaagttaa
    69541 atgtgaagcc gtagtaatgg cggttggggc aaaaagtaac actaaagtcg ttgaactggt
    69601 ggcacgcctc ggttacgaac attatgtcgt cggtgatgca cagcaaccaa acaggatttt
    69661 agaagcaatc tggggcgcca atgaaatagc tcgcaccatc tgaactgcag ttttatctcc
    69721 cttgttatct aggttatcta taaaaaaaac ccactctaaa aagtggtttt ttttactgca
    69781 aactaaggct atttatttag actctctcct gcagatggct ggtgtcattg agtttggcaa
    69841 gataccagca gttgttataa ttatacaaag ttgtcagaga ggcagtatcg atttgcagcc
    69901 gcccagctgc tgcggctggc atctgcatcg ccagagtcac cgctgtgcgg ataacaccgc
    69961 cgtgagtaac aatcacggcc gtctcttctt gctccagtct tgcgaccagc gtttgcagga
    70021 aatcgtcaac tcgcgctgcc agagctgcag ctccttcacc agcaggagga gtattagtga
    70081 cgggatcatt acaccaagct gcaaactgct tttgcatcgc ttggtccatc tccgcgtaac
    70141 taagcccttc ccattgccca aaggacattt cgcgcaaatt atcatctagc tgcaaaggca
    70201 gctcaaaacg ggtcgccagg ggagcagcaa aagtttgtgc tctttgcagg ggcgagacaa
    70261 aaatatgtgc aggtttctct aaaaaagcag ccgccaattg ttccgcctgc cggcagccca
    70321 gctcacttaa agcgacatca cttaaccctt gcaggcggta ctggcgattc cacagggttt
    70381 caccatgccg tacccagatt aatttcacac taccctctcc cttgccctaa tttcacctgc
    70441 caaccgcatc attacagaaa taggagcgga gcaatgagca gcactgccaa ttcaaacagt
    70501 tcgcagacgg caccaagaat gtcgccagtc acaccaccaa aattaagttt aaaaaagtaa
    70561 cgtaaaattg caaaaacaac gattagggct aaagttaaag gaagcatctg ccagccccaa
    70621 gcagcaaaac caagtaccag gcatagcaat atcgctaaga aaagttgtgt agtgcctaat
    70681 tcgtccacaa agatcttccc aagaccaccg ctgggccggg catagtcaca ggtagccatc
    70741 aaggctacca tcacggtacg accgactagt ggcgcaataa tcagcagccg agcgtcagct
    70801 gccaaagagg ccacagcaaa cacctttaac agcatcgaaa gaactaatac ttgagctcca
    70861 aaagcaccga cacggctatc cttcatgacc tctaatcttt tttccagatt gccgcgtacg
    70921 cccagaccat cagctgtatc cattagaccg tctaagtgca agccggcact aattagttcc
    70981 cagagggtaa cagtgagcgt agcagctaaa agtgttgaca aaactgtgtt tgcccaaagc
    71041 caaaagccat agagcgtcaa gccgatcagc gcgcccacga tgggataata ggtagctgaa
    71101 ccgactaggt catctgtcgt tacttcggta tcgccgccaa acggataaac tgttaaaaag
    71161 cgtaaagcta caaaaaagcg tttcattact aggacccctt tccacgaaac ggtcaagacc
    71221 gttccctaca caggaacggc accgttagta caagcagtaa tttttgaggt tattctacgt
    71281 tttggtaggc ttttacgcaa aggggatagc cggaaacggc caaccaaagt gaatctgcgg
    71341 cacgcgctac ctgttgatta gcccgtcctg ccatgtcacg gaacacacgg cccatgtaat
    71401 tatctgggac aataccacta cctacctcat tagttacgac gacggtacga aatggcttgt
    71461 tcaaggcagc agtacaaaaa cgagctaatt ccgtttggat ctgtttttcg atatccagat
    71521 atttttcgtc actcatgttt tcttcatact gcgctaatag ctggttagtc aaccagatgg
    71581 ttaagcagtc cacaatgacg gtaactgtcc ctgccgggac tctctccaaa gctcccgcta
    71641 aatcctttgt ttcctctact gtcgaccaat gtggcgggcg tcgttctttg tgcagagcta
    71701 cgcgcagtgc catttcttca tccaacacgg cggcggtagc aatatatgtt gcaggcccgc
    71761 ctgctgtatg ggccaattgt tcagcaagac cgcttttccc actccgggca cctccggtaa
    71821 ttagcaccag cttacctaac ttttccatat ccatgtatag atttcctcct taggggtggc
    71881 tttctgtctc gttactgctt actcctgact gctggcagca gcttacgaat aaatgacaag
    71941 tccagatttt cgcgtagtgt ttgcgcaagc agctgatatg actgctcccg cttttcctta
    72001 gtggcagcgc ttgccgcctc cgcctgaggc ggcagggcca gcccatttag atggggaatg
    72061 acaccgatca ctggcttgtg aatgatctct tccagcatct ctaatcccgg ctgcagcttg
    72121 tctctaaaac cacgaaaacg attaacaact acggcctgaa tcagctgccg ctgctccggt
    72181 ggtaaaagtg ctagtgtccc agcgacataa gcaaacatgc cgccgcgatc aatatcgctt
    72241 accaacagga caggtgcatc aagtgcttta gctaaaccca tattgggcag cattaactct
    72301 ggacgatgta attctacagg agtcgcggca ccttcaataa ttaccacctc gtagtgcgag
    72361 cgcaaaactt gcagggcatc cagcaccagc gccaaagcag tatcgctgac agccaagagc
    72421 tcctgcctcg tcagctcacc cttaatcaca ccttgcgctg tcagctggaa agaatcttcg
    72481 ctaagcggtg ctaaaagcag tgggttcatc tccacgctgg gtgtcacacc ggctgcttct
    72541 gcctgaagat actgcgacca gctaatttga cctttgggca ggtcgacagt ctcagcgccg
    72601 atagtttgcg cacagaaagg ggctacgcgc caaccttctt ctgccaacag gcggcacaac
    72661 gccgtacaaa ggatactttt gccggcatca gcagatgttc cttgtaccat aatcacagtg
    72721 ctcatgcctg atgctctcct ttctgcgctg ccaaacccaa cagccggtac agcgccgaca
    72781 gatccaagga gttttctacg gcgtcagcta aggcatcaaa ccgctgtgct ctgtcttcta
    72841 cagcactttc accgagcggc tgccagccgc gacgttgccg taatttattt aaccaggccc
    72901 gccgaaaacc aggcctgtca aaaacaccat gtaaatatgt tccccagaca ttaccctggg
    72961 gagaaatagc gccatcttct ttgccatccg ctaacacaaa ggcagcggga cagtgcgctt
    73021 gacgtttagt ttgccccata tgtatttcat aacctgtgac agcagctccc tgggcaaaag
    73081 gcagctgcgc tgcagcagat gctctgccta ctgttttttg tggttgaaaa gatgtggtca
    73141 cgggcagtaa acctaaaccc gctaaagtag tagcttgtcc agcaaattcg ctgccttcgg
    73201 cgtcgctgag ttcttcgcca agcatttgat agccgccaca gataccaatg actggcagcc
    73261 cctgcctcgc ccgtgcaatt accgcttcag ccagaccaag ctgctgtaaa aacaataaat
    73321 cacgcatact gtttttagtg ccggggatga tgatagcatc agcgccggca agatctaatc
    73381 cgtcataagt atagcttaag gaaacatcct cctcactacg caaggcatcg aaatcagtaa
    73441 aattggcaat atgaggcagg tgcagcacac agatatttaa acagccttca cctttaaccg
    73501 tgctagagag ggtatcgtca agagctacac catcttcttc cgccagataa gcttcctcta
    73561 aatatggcac cacgccgaaa acaggcagac cagtttgtgc tgtaacttct ttcagtgcag
    73621 gagctaataa gctgctgtca ccgcgaaatt tattgataat aaaaccagct acccgctggc
    73681 gatcttcctc cggcagcagc atcaccgtac ctacagaggc cgcccaggct ccaccccggt
    73741 ctaaatcact gaccaaaagt accggtgcct gcgccaaggc tgctgcttgc atgttggcta
    73801 catcctgctg ccttaaatta atttcagctg gactgcccgc cccttccagc acgataattt
    73861 catactcttg gcggaggcga tctaaagctt cagcaatgac ctgcaaacta aactcgtggt
    73921 actcctgctg agtccgatgc gtatgtccca tcataccata aacttggccg cgcacaatca
    73981 cttgggcttc cccaggcgcg ctcggcttaa tcaaaatagg attcatatcc actgtagctt
    74041 caatgccggc cgcttcagcc tgctctccct gggcccgacc aatttcaccg ccatcactag
    74101 ttacatagga gttgagggcc atattccagc ttttaaaagg tgccacccgg tagccggcgc
    74161 gggcaaaaat tcggcataag gcggcagcaa tagcactttt gccaacatgt gaagcagtac
    74221 cttgcaccat aatagttact gcctgtttgc ttgttttagg cattgtcaag cggcagcctc
    74281 cttttgcagc attatttaaa gaaaacattt cttaattacg gcaagactaa taatagctta
    74341 ctttaataac ctaatgttaa tctgaacttt gacaaggagg ctgcgctatg tttgtggagc
    74401 taccgctgct ttttctcgtt tttgcgctgc tctatttaat tgtagaagca gcaaccacca
    74461 tgctgatgat caccggtctc gatcagcata cagcccgttt tcaagctatc tccatcatta
    74521 ccggtaccgg ttacacgacc tcggagtcag aattaattac tcgtcatccc gtcaggcgca
    74581 aaattgccat gttcttaatg attgttggca gcatttctct ggcggtagtt atttccatcc
    74641 tcgtcaggct cctcagcagc aatgtctcag gcgttgaatt attcttgaca atcgctttac
    74701 tgctaattct caccctgatt ttgcgcagtc gaccagtgtt gttatatttt gataactatt
    74761 tagagaaaca aatgttgaaa cagccctata ttcataaacg tacagttgaa gaattgctga
    74821 aaatagatga acacttcagt attgcagaaa tacaattaag tcaaaatgcc ccttggcttg
    74881 atcagaccct cggcagggca cggctgcgcg atctgggcgt gctggtgctt tcaattcgtc
    74941 ggcgtgacgg caatttaatc cgtgcccctc agggtgcaga tgtgctgcgc gaaggtgaca
    75001 ttctattggt ctacggccgg ccgcgttaca tcagtgaaat cattgagctg gccacttgat
    75061 gaaaattgtt cagctaaagc acggtatagt tcagtttttt ccctgatgga ttgagcctgc
    75121 tgttgaactt ttgttaacag ttcttgtccg ggaccgcctg taaaatcacg attttgtgct
    75181 ggtttttctt cgatcatata cacaggctta ccagcggcag ctgcttgcag ggcagcctca
    75241 aggtttaaaa ggttgccgtg tcccagaggt atttctgtca ggatcactgc ttgtgcagtt
    75301 tccagcatag ctaaattttc ctggtggcgt tcaggtgaaa cagacgaaaa tggggcttca
    75361 gaaatcactg gtatacttaa ggcacgtgcc gcctcccaat cactgtcacc cacactcaag
    75421 acgccggcag ttaccttata cccggcatca cataaatcgc gcattatttc accgccgctg
    75481 cctccgccag caatgacatg tatctggtga ttttcaccgt gcttgttttg ttcacgcgtt
    75541 ccgggcaaaa gcgaaatttg tggtgtgcca aaaacaggat gcggtgtcac caagacgcgg
    75601 gtagcgtaaa ctttttctat attgtccgcc gttaacacat ctgtcggctg accacaggcc
    75661 aaaattttac cttgatgcat cagtactagt tcatggcaaa aataagcggc taaattcaaa
    75721 tcgtgtaaca ctgccaccac ggttagtcct tccactgtgc ttaagcgctt caccaaatcg
    75781 agaatttcct gctgatagcc gatgtccaaa tgtgaagtag gttcatccag cagcagcacc
    75841 ttaggttgtt gtgccaaggc ccgagcaatg gttacccgct gacgttcgcc gccgctcaat
    75901 tccaggatac tgcgatctcg caaatgagta cagccggtta cttccagggc atgccgcgct
    75961 atttcatagt cagccaatga ttcagactga aagcgcccca gataaggggc acgccccatt
    76021 aatactacat cttctactga aaactgataa ccgacattag tgtcttggga gacgacagcc
    76081 atagtacgtg ctaattcttt ttgcgtaagt tctgacagtg actgctctcc aattctcaca
    76141 caaccagctg ttggctgcaa aatacgggat aacaccttta agagtgtggt tttaccactg
    76201 ccattgggtc cgataattcc cacaaacttg cctgacgata cgtgaaaagt ggtaccctca
    76261 agcacctgat gattgccata tgtgaaggag atatcttgaa tttctattct cataataacc
    76321 tcacaagcga atctcgcttt ttttctttct caaaagatag ataaaaaacg gcgcacccac
    76381 aaaggctgtg atgatgccta cgggaatttc cttggggatc atgactgtcc tggccaccac
    76441 gtcggcccag attaaaaagg tggcaccgac taaggctgag gccggcagta aaacacgatg
    76501 atccggacca gtgacaatac gtacagcatg gggaattatt aaccccacaa agccaatcat
    76561 gccgctcaca gatacagccg ccgccgtaat taaagaggcc gttactaaga gtaccctttt
    76621 tgtccgctca acattgacgc caagggtcag agcggcttct tcacccatca agagaacatt
    76681 taaatcacgg gcataagtaa aaagaataat tgagccaatg agtagataag gtaaaacaaa
    76741 ccagatttcg gaccaggagc gcatcaataa accgcccgat aaccagaaga aaataccttg
    76801 catatcttcg ccggcaaaaa ccattaatag ggaaatgaca gcagagagaa aacttgagac
    76861 tactaccccg gctaagagca gggtgaccac aggcacctga ccgcctattc ttgctagatt
    76921 atagacaaag aacatcgcta aaaaggctcc ggcaaaagca aatattggca gcatttgtgt
    76981 gaagcttatt tggtaagtct gctgcaataa catcgcaatg gttgcgccga cggccgcacc
    77041 actggaaaca ccaatcgtaa agggatctgc tagcggatta cgcagtaaac cttgaaagga
    77101 caccccggct aaggccaaag aagcgcccac taacaaacct aacaggacac gcggcaggcg
    77161 cagattccaa atgatagcct gtgtcgtggc gggaatatca ctactctgcc acaagccggt
    77221 taaacgtgag ataataatct gaaatgtttc ccggggggga atggctaccg cgccaaccgc
    77281 ggtagccaaa attactgaga ccacaagtgc agacaatagt aacgtgtaaa gaagatactt
    77341 atttctcgtt ttcatggcta ctttgcaaag acctcataca gcagctccag accttgtaca
    77401 taacgtggcc cgggacgtga tactgaatta gcgtctacgt cgtaaacctt gccgctttta
    77461 atcgccgata aatcttgcca tgtttctctc gccagcacct catcgcgcat ggggaaagta
    77521 caaataatca cttcagggtc aagttcaaaa aatgcctctt ccgaaacctg gaagtacgcg
    77581 tcatcaatct cagacgccag gttattccca ccggcagtag tgatcatttc tgagaggaaa
    77641 gtgccttctc ctaccgtgta aaggacatta gtatcaagta agacaaaaac tttagcacgt
    77701 tcttcagccg gaatttcagc cacttttgtt tctaaagcaa gccgatccgc ctttaattgc
    77761 gtcaccagag cttcgcccgc cgcttggtta tcaattaact tggctactgt gacgatggat
    77821 tcttcgattt catcaagtgt ctgcggatca acaacaaagt atggtgtacc agcctgctct
    77881 aggaaatcaa atgtttgctg taaagtttca cttttcccga ctaacactaa atcaggctgt
    77941 aaggctagga tgctttctgg gttgaggtta tacaggtcac caataacttc tttctcctta
    78001 gcggctgctg ggtagtcaca atatgtagtt acaccaacta cccgctcacc tgcatccagg
    78061 gcaaacaaaa tttccgttaa gctcggcata atggaaacaa tgcgttccgg tttagctgct
    78121 agcgttactt cccgccccgc agcatctgtc agcgttaacg gataagtttt tccggtatca
    78181 gtgtccccgt tagcttcttc accgccattt tcttgaccac aaccagcaac cagtgtcaaa
    78241 gtaaacacca ccaataacat cacaaccaac cagtttcttt ttctcatctc tctttttctt
    78301 cctccttatg gctgaaattg agaaaggcgc atcccgcagt ttccgcttgc gctccttccg
    78361 tactggcaat gacgaaactt cgtttaaagc cagtaacaaa aaaatcccag tgctcggact
    78421 gagaaagcat aaaaaaatca cgtttccctt aacccgaggg cacgatacgg ctggtgaaga
    78481 ggcaggtttc ctggctaccg ctcatcacta tacagcgcct tccccgttaa acgagtggct
    78541 tgttgcagta tagctcacgg atacagtggc gggaccgctc aggtttcaca cctgattccc
    78601 tttatgctca agagagcacc tattcacaca cgttatggat ttaaattaat tctatcagac
    78661 atgacaagta aatgcaatac tgtttttacc tagattaact ctttactcct gcctgccttt
    78721 ttagcgcttt cacttcggcg ccgtttaagc gacgccactg gccttttttc aaaccagtca
    78781 aagttaatgg acctacacgg acacgttgca gagatactac cggatggttt acagcctcta
    78841 acatgcggcg tacctgacga ttacgccctt ctttaatggt caaagaaagt gtagaagtag
    78901 ggcggccgcc tttaatcagc cgtactgcag ctggtgcagt ataaccgtcg tcgagcttaa
    78961 cccctttttg taatttggtc agcgcctcac tgtcgggcaa atcatgtata cgcgccaaat
    79021 actctttttc tacaccaaaa gaagggtggg ttaagcgatt cgccaactca ccatcattag
    79081 ttaagagcag taacccgctg gtatctaaat ccagccgacc taccgggaag accctagtcc
    79141 ccacatcttt gactaaatcc tgaactgtct ggcgcccata aggatctttt agcgtcgtca
    79201 cataccctat cggtttatac agcaaaatat attccttttt ttctaccggt ttgacgagac
    79261 ggttgtccac ttctacttga tccacggccg ggtccacagt tacccccatt tccgtaatcg
    79321 tctgaccatt gaccttcacg cgtccctccc gtatcatctg ttcaccagcg cggcgggacg
    79381 caactcctgc catcgctaaa tatttttgta gtctgactgc catcgttact acccctctat
    79441 ctgtcgtcac gctcattcgg ttccttgtcg cgtgcttttc agtactgacc actaattgtg
    79501 atgctcaaat tagctggctt ttctccccat catacaagat tcaaaccgcc ggtgcaagtt
    79561 gttagtgaaa aacaagtgta cagattacga tggaggcaat aaaagcagcc aagtcagcca
    79621 gtaaaccaac tgtcaaggca tgacgagtct tgcggatacc aacagaacca aaataaactg
    79681 ttaaaacaaa gaaagtagtg tctgtactcc cctgcatcgt tgaagctaaa cgaccaataa
    79741 aggaatctgg gccaaaagcg ttgagaattt ccgcagtaat ccctaatgca ccgctgccgg
    79801 aaagagggcg cataattgct aggggaataa tttcgcccgg tacgttgaag cgggttagca
    79861 gcggcgacaa taattttatc agcagttcca gcgctcccga agcacgtaat aaaccaatcg
    79921 acaccaacat ggccactaaa taggggatta aactaaccga ggtggcaaag ccctcttggg
    79981 caccctcgac aaaagcatca aacacatcta cctttttgac tacggcataa accagcacga
    80041 tgaaaatgaa gccaggaata acccaggccg ctgctgtctg cacaattact tgcagcattt
    80101 tccttacctc cttttacgtt ttctacgggt aaggccgcga ataagtttat ccagaataat
    80161 cgccgtcgtg gtggcacata gcgtggccag gatggtcgtc cccacaatct ccgtggggta
    80221 aagtgagccg ctggcagccc gcagtgccac aactgtggta ggaattaatg ttaaagcgga
    80281 ggtgttaatc gctaaaaaag tacacatttc atcactggcc gtttgtgggt cctcattgat
    80341 gctctgcagc tcctgcatgg cttttaaacc aaagggagtc gctgcattac ccatgcccag
    80401 caggttggca ctcatattca tcagaatggc atgcatggcc ggatgattgc gcggcagccg
    80461 cggaaataat aaccgtgaca gtggtcgtaa taatttaacg atccactcga taaaaccgct
    80521 tttctcaatt attttcatca tgcctaacca aaaagtcata atactgacca tagaaaaaac
    80581 tacactgaca gcttgctcgg ctgaggagaa cagcgcatca gtaaatgttt caatgctgcc
    80641 agtaagtgcc gcagtgacaa aggctatggc aatcatggca aaccagataa gattgatcac
    80701 tttaacacct ccccgcccca gttgtcttta aaagatacgc tggaaaaact tgcgcagact
    80761 ggcaaataag cccttactct ccacttctgc tgccgccaca agtggtactt cgctgacttg
    80821 ttgggtacca aaatagatgc gcaaggtacc tagctgctct cctttgctga tcggcgcctg
    80881 tacttcttcc ggcacctccc aggcatagcg caggaggtct gcttcattat ctttaagcgg
    80941 cagcataata tcttcactgg ccaccaagga tattttttgg gccgcacctt tacgtacagc
    81001 ggctgttttt aatggtgtct cagccttcac gatagttttt ctttgataat gagcatagcc
    81061 aaaatcaagt aagtccgtcg tgtcttccca catttccggt gcatttaaaa ctacagttac
    81121 caggcgccag tcatctctag ccgcagcacc aacaaaacag cgtccggccg gagtagtcca
    81181 acccgtctta attccctcgg ccccctcata catcgtcaac aatttattct ggttacttag
    81241 taggcgatca taggaacgac ccgtccaaga aattttcttc tcggctgtcg ccgcaacttc
    81301 gcgaaattta ggcagggtca tagcatgtac ggcaattaaa cctaaatcgt aggcagttgt
    81361 gtaatgttct tcatgatcta aaccatgcgg attcataaaa ttggtatgta ctgctcccag
    81421 tgcatgtgcc ttctccgtca tttgctcagc aaattcctca actgaacctg ctaaaaactc
    81481 ggcaatggcg gcagcagcat cattaccaga acgtaaaatt aagccaaaca gtaattcttc
    81541 taaagtcttc ttttcccccg cttccagatc aacactggaa ccttccacta acgaagcata
    81601 ttcacttact gtcacaactt ccgccagttt gcccgactct acggccaaaa tggctgtcat
    81661 aatttttgtt gtacttgcca ttggcagctg ctcatgtgca ttcttactaa agagaattct
    81721 acctgtggct tgatccagga ccactgcagc tcgcgccgtt acctgcggcg gctccgccgc
    81781 ccagccgctg cctggctgta acaacaacag acttactaat aacacaatga taaatttacg
    81841 catacccgac cccccaataa actcaatacg aaatcatcaa tataaaaaaa gccatgctta
    81901 ttgttattcg catggctggg ggaatatgaa acttgtcttt agatttgata atgattaagc
    81961 tgtgcatcag cgggcggttc tttttgcagc ataccctgaa cctgattaat aatctgtggt
    82021 gccatatcca tcaggcgatc aaacatcact tgacggtcaa caggcagcat tttcacgtga
    82081 ccatcatggc tcacaatgag aaaagctaca ggctgtacag aaacgccgcc gccgctgccg
    82141 ccgccaaaag gcagataatt tacatcttct gcttttttat ttttcttttc ctcttccaga
    82201 tcattatcaa attcgctgcc gccggccgca aagccaaaag tgacacgaga aatggggata
    82261 atcgtggtac catcctgagt ttgtacagga tcgccgataa tggtgttgac atctaccatc
    82321 tctttgatgt tttccatagc tgttttcatt aagccttgga tcgggtgttc ttgcatcact
    82381 aatctttacc ccctcgtata atagtcgaca tgaataacag gctgccaagc ataaaatgcc
    82441 cgatgcgcaa ttttactacc gcatcaaaat agactttcgc cacagtttgt gggctgaaat
    82501 ctggcttaaa agcaagtact ggtcgttctt taaaacgcag ccttttttgc aaagcgggga
    82561 caaatattac tgccacactc caaaacaaac ctgtaagcaa ccctgttagc gctgcatcat
    82621 tgagaccgac atgcactcgt aaaacgaact tttctatggt catagcctgc ctgtaaaaat
    82681 tactgagata cgacagcata tctttgttgt ctttaatcca cgctacatac aatttgacct
    82741 tttcataatc aaggtcaaaa acagagattt tttctttttt ttcgccgact aatttgtcct
    82801 ctcctgcttt tagttcagct tccagtgtca catcaaacgg cgtatcttgt tgaattaacg
    82861 gcacttccac attaaaacgc aacagggaaa aaaatgtatt aacgcggatg tttaaaaagt
    82921 catctttatt gtccctaaca aaaaaaagat ttatgcgtac cggaatcaac tggagcaaaa
    82981 gcatccccga caaatataac ggtaacaatg tcaaaatcca tttcatgcgc ccaccaacgt
    83041 tgcacagttt ctcttaactt ctccttaaac aggaaaaaaa tgcacacttt aatctttaaa
    83101 ctaaaaaaaa gccctgtagt aatttaccac tacagggcct gtgatcttta tcttataact
    83161 ttttttctac tttcgctgct tcataaagtt tagagtattc ttcttgcata ctttcgctta
    83221 cttttttctc cattaaagct tcctcaacct gctctttaac cgcagaaaat tcaacttctt
    83281 gcgcttcctg tcgttctaga acctcaatca catgataacc atgttctgat tttactggtt
    83341 cactgtactc acccacaggt aaggcgaaag ccacgtcttc gaattccttg acaaattggc
    83401 cgcggacggc taaacctaaa gcaccgccct tactagcgga gccggtatct tgagaatgtt
    83461 ctttagccaa ctctgcgaaa tcttcgcctg cttttaacga agccagcatt tcattcgctt
    83521 cttcttctgt ttccactaaa atatggcgca catctacctt ttcggggata ttgaattctg
    83581 cttggttttc tttaaaatat tcttcaactt cagcttcagt tacttcaatt ttactgcgga
    83641 caatagctga aagcagtaac tcgttacgga tattctcttt gagggtttct tcagtaatac
    83701 catactgggt taaagcctga tcgaagtatt ctttcatgcc gtagtagctt ttctcaatca
    83761 gctggtcaat ttcctgctga acttctgcat ctgtaacttt gatccctgat ttagctgctg
    83821 cctgagccac aacgcgctcc gaaattaatt gatctaaaac ggtgccgccg ccgttagcca
    83881 caaaagcctc atagagatca ttcttagtaa ttttttcacc attgaccgtt gcgactacct
    83941 cgctgcgttg gttaatcacc actgcaccaa tcacggttgc gacaagcaac agtagtagga
    84001 ctagagatac tgttttatta ttcatcttcc acctcttaat ttttgcagtt cccacagaac
    84061 tactattttt actaacccat agcttctatt gtagcaagta aatgttacaa acttatggac
    84121 tgtcctttgt ttttctttaa tgtttgggct ttaaatacat aggaaaagga cagaaaatct
    84181 atcccgattc cccgccctaa aatattatta aaactgcttt tcttcattat ctgtcaagcg
    84241 aggcagctca ctgagatcct tgagcccaaa gtacttaagg aattctttgc ttgtaccata
    84301 cagtatcgga cgtcctggtg cgtctttgcg gccgacagta cggataaaac gacgtctctg
    84361 cagtgtgttc atgaccccct caactttcac cccgcgaatt gtttcgatgt ccacgcgagt
    84421 gacaggttgg cgataagcaa taatgcctaa ggtttctaag gccgcttgtg ataaagtata
    84481 gctcttttcc cctttaaata acttttctat gactggcgat aagtctggtt tagtgctaaa
    84541 ctgatagccc ttagcaatct ccatcacttg taaaccatgt tccgatgaag catattcctg
    84601 ctgcagcagt ttgagcgtct cacgcacatc tttagtctcg gcaccaataa tttcagcgat
    84661 tttagccgga ggcaacggtt cttccgctac aaaaagcaaa gcttctacca gcggtttaat
    84721 ttccatactt ccacctccag cgtttatttt agtagaacca ctaattcccc aaaagggttg
    84781 gactgccaca cactcaattt gcccagacgc accaattcca agagagccac aaaagtaaga
    84841 attatttcag ataaaccggc tgtctcggta aacaaaccac taaagcttaa accatctttt
    84901 tggccctgca gctgtgaaac cagctgcacc atcagttcct ctactgaaaa atcatcagcc
    84961 tctacttcta gtggttgacg ctcgtgttcg gcaagaagtc tttggaaagc ctgcatcaaa
    85021 gcggggagcg agacaccaga gatgacaaaa tcatcttccg ggttcaccaa gaccaccttt
    85081 tcgcctgtta gtgaacgcac aaaaacacgc ttttgtgctg tttcataagc acgcagctct
    85141 tgagccacgg cgcggaaatg ccggtattcc aataaacgct ggatcaactc ttcctgcgtc
    85201 tcaatgtcca tgctgtcttc atcatccagc gcttcatccc cgatactgct aggtaaaagc
    85261 ttttttgatt ttatttttaa taaagtagct gccatcacca agaactctga agcaacttcc
    85321 aagttcagct tctgcatcag ctgcagagtc tccagatatt gtgccgtgat ttcagcaacg
    85381 ggaatatcat aaatgtctac ttctgccttt tcgatcagat gaaaaagcaa agcaaaaggt
    85441 ccttcaaatg tcggcaggtt cacataatat tccaccgctg gcatcctttc tctctgtgac
    85501 aggggacggt tccttgtcac gcccaactca ttacctttaa ccattatctg ctaggctaaa
    85561 aatcatacat cagggatagt cttgatcgtc agtgagtctc ccagctacta caacttaaga
    85621 ttaacaattt cccgcgcttc cgccatcgtt tgattagcta tggccgaggc gcgcttggcc
    85681 ccatccagca aaatatcgcg aatataatct cgctgacctg ccaaggcttg tcttctttca
    85741 taaattggtt ctaggaaggc tactaaacga tccgctaatt gctttttaca ttgtacgcag
    85801 ccaatttcac cacggcgaca ggcgccttcc acactggcca gctcgtccgt attaaaaaca
    85861 ctatggaaag tatagacagc acagatatca ggattaccag gatcatcctt acgtacacgg
    85921 ccgggatcag tgaccatctg ctgcactttt ttccttacct cgtctggtgc ttcagacata
    85981 ttgatggtgt taccatagga tttactcatt ttacgaccat caataccggg cagcattttg
    86041 accacgttta aaatagtttc tggttcaggg aaaaagttag ttttatagag aaaattaaaa
    86101 cgtcggccaa tttctcgcgt tatttctagg tgaggaattt gatcttcacc caccggcact
    86161 gcctccgccc gataaagcag aatatctgca gccatcagca gcggataacc gagaaaacca
    86221 taggttccca agttcttttc ttttaactgc tgaatttgat ctttataggt cgggacacgt
    86281 tccaaccaag atagtggcgt aatcatcgat agtaatagat gcagttcagc gtgacctgtc
    86341 acatcagact gacggaaaat aatacttttt cccggatcaa ggccggcagc aagccagtcc
    86401 attaccatct gataggtgtt ttcctgtaat cctgacggat cttcataagc tgtcgttaaa
    86461 gcatgccagt ccacaacacc aaacaaacaa cgataatcat cttgtaattt aacccagtta
    86521 tctagtgcac ctagcaaatt gcccagctgc agacgacctg tgggccgcat cccactgaag
    86581 atagtttttt gttccattgt tgtacccctt tcagataccc ccgcccgtta acagcacgac
    86641 gatggttgta ataacattcg acacaacacc cgctaccgga tataggatcc ttgataacgt
    86701 gcgcgtaaag accagtaaca tgagaatcaa tggtccatat tgttctagct gcgcaaattt
    86761 ataagcagca ctgcgtggta aaaagtaaaa caatactttg gagccatcta acggcggaat
    86821 cggcagcaaa ttaaagacac ctaaaaatat attatacaca aaaagtaact gcataaacgg
    86881 caaaatataa gttgaacgca aaggcggcag cagcattaac agcaaagctg tgaaaaatgc
    86941 taaaagaaaa ttggttgcag gcccagccaa gcttacccat agtaaaccac ggtgacgatc
    87001 accatgaaag tatagcgggt taatgggtac aggtttagcc cagccaaacc cgactagcca
    87061 caaggctaac agccccagcg gatcgatatg cttaagcgga tttaatgaga gacgcccctg
    87121 ctgcttggct gtcggatcgc caagcttata ggctaccaga ccatgtgcta attcgtgtaa
    87181 ggtcaaagct agcagcagtg cagggatacg ataaaatagg ccgcttgtga caaagcgtga
    87241 aatcaaattg cttataggca agttaattct cctttcccca aggtgggtca cgctgaaatt
    87301 gttgttgcac gaatgccagt tcctcttctt tattgccgac taggccgtca actctggcag
    87361 cacgtacagc tgtcaaaatt tcctgataag ccggccctgg cggcaagccc agcacttcca
    87421 aatcatgccc gctgatttca atctggatgg gcctaatctt ttcccaataa aagtagatac
    87481 ggctccacac aagactgtgc ttactgacga ctaagagcag cagcattgtt tctagaggct
    87541 gcccccctaa ccaatgttct aactggctag gctttagttc ttcgtctgtg gtcagctctt
    87601 cctgcagctg gggtactacc gtgatcgcat tcagtacccg ccgtatggta tcccgcggta
    87661 aacgcagacg ccgacataaa tgtctggcct cctgaaagcg caagtccagc attaacgacg
    87721 ctaaataaag ggctgcaggt atcggcttag cgtcgagcca ggtgcgctgt acgcaggcca
    87781 acacctcttg cacagtaaaa agtctttgct tcagcagttc atgataacga acgccaggga
    87841 aaatactgac atcaagaccc agctcgaaaa tacggttcag catggccgcg atcttttttt
    87901 cctgaaaaat taaggataac tcatcgtaaa gacgttcttt gctgacgttt tctagaacac
    87961 gtgttttaac tgcgttttcc agtaaagcct gcgtcgtttc ctccaactga aaagcaaaac
    88021 gttgttcgaa acgaatggcc cgtaagagac gcaaaggatc ttccacaaaa cttaaattat
    88081 ataaaatacg tattagtcca ctttctaaat cacttaagcc atggaaaaaa tctataagtt
    88141 gaccgtagtg aggcatattt aaactaacgg ctaaagtatt aatcgtaaaa tcccgccgga
    88201 acaaatcatt ttttagactg cctagttcca cttccggcat cgctgctgga cgagcataaa
    88261 attcctgccg cgccgtcact aggtcaatct gcaaatcatc acgcatggtc agcgtcaccg
    88321 taccaaactg ttcatgcttt ttgatttgtc cacccaaaac ctgatgtaac ttttccccaa
    88381 agggaatcgc cagtggttct accgccaagt ctaaatcatg agtttccaga cctaacaaca
    88441 aatcacgcac aaaaccacca acagcgtaaa cctttgactg ctcacggtcc gccgtttgcc
    88501 cgattaaata aagtaaactt tgtaattttt gcggtaggcg ttccatgaaa agggttgtaa
    88561 tatcctgacc agcactagga aaagcaggtg attctccttg ttgttttatc ttcattttgc
    88621 ttccttccag gctatctagc aaatctgtac gggtgatgat accaattatc tcttcaccct
    88681 ctactactgg taagcgtccc atatcctttg taatcatgat atgctgcact tcacgcagtg
    88741 acatttccgg agcaactgtg acaggcttgc gactcatgta gcccttgaca ggcgcatgac
    88801 ctaaaccatg ccgcatggcc ttttctaaat cacgccgcga gataataccc accaattgtc
    88861 cttctgaaac gaccgggaaa ccgctgtgtc cataacgcag cattaactct tgcgcctctt
    88921 gaatgctggt ttcaggagaa atactccgta ccggtgctgt catcatctca cgcgctgtgg
    88981 tgactggttt aacttcctca gccagtactt taatcaactg ttcctgcaca gcagccaacg
    89041 tagtatcctt gaccatcgct gaggcggcag cacggtgtcc cttcccgcca aagacagcca
    89101 ttaagtcgga cacattaatt tcttccacac gactgcgacc cacaagatat actttattac
    89161 ccattgccgc cgccgtcaac attaaatcta catcctcaat ttctagaagc ttttgagtta
    89221 acgaacctaa cccatctaca taatcaccgt tagcagccgt agcaataccc acacgcacac
    89281 cacgcagctc atagtattca gtggcatcta acagttcatc taataagtct ctttgctgat
    89341 cattaagggg ctgctttaag aattgattaa ccacacgtaa attcacacca acctgccata
    89401 attgatgcag cgccgccaca tcgcgttcgg ttgtgttttc aaacgtaaaa ccacctgtat
    89461 cttcataaag acccaaagca aataaagttg cttcaaaagg agaaatggtg atgccttgtg
    89521 tagagatctt ttcccagagc agagtaatcg tggcaccaac attacctact accatatact
    89581 gagcggccag atcatcagct gactctggat gatggtcata caaatggatt tctgtaagct
    89641 ggtccaagta cttggcaaat gaaccaagac gctggcgctg ccgggtatct gtaatcacca
    89701 attgtttcat ctcatctaaa gcaagctctt tacttgtttt aaacggtaac tgatctttat
    89761 atagtgaaac atactcttgc acattcttat tcaggctcgt gggaagaacg gcctgcgccc
    89821 cgggataaag ttttgtggcc gcgaccagag aagccaagcc atcaaagtca gcattaaggt
    89881 gcgttgtgat tacctgcaag agccgtcact cccctcaaat cttttcccat tataccacat
    89941 ttcccctaac tacgggaata tcattagcag aatttatcga cagacacgcg cgacagggga
    90001 cggttctttg tcgcaccagg gattggcagc tatgttatta atatcctcca gcaaggagta
    90061 agtttcaaaa acgttcttaa ctgttccaca ggacaaacta cccctttaca ggtacacccg
    90121 tcacctaaca attttaaaca agaaaaagag cggtctttga ctaccgctcc tcagttactt
    90181 gggctggggt tgggctcatt agcttaaacc cgactccaag tttttttacg ctgctccagc
    90241 aggtcgagga aggcctcaag gcgggtttta aatcctgctt cacctgattg ttcatctagg
    90301 gagaaggata gcactggaat gccctgttcc ttgctgactg taggtaaaat gctttgcgcc
    90361 acgatttcag gggtacaggt aaagggtaag atgtgaatta ccccgtcata accgcgccgc
    90421 gcataacgga ctgttgcacc gatagattcc cagccatgac cacccaccgt gttgttcaga
    90481 taagcttcgg ctgctctttt aacggctaaa ccacccttta aacgcatgga acttagcagc
    90541 aaatgctctt tcatccactc agagatataa agggagcgtt ctacctctac acccatttca
    90601 cctagaattt tctccacctc tagattggca aatggttcaa gcaacatata gatttctccc
    90661 actagcccta aacgtaacac agacttttgc cggtcttgtg gtacagcttt taatattgct
    90721 agcccctgcc taagcttctg attaactgct gccggcgttt gtgcctctcg cagcagagcg
    90781 atactttttt gataagcttt agtcgtggcg tgcagctcca cttcacgcgc tcgcacctgt
    90841 aaggaaagag catgcaattt gtctagggcc ttcaccttac gccacacggt atgtagggca
    90901 cgcatcattt gtgcccaact tgttgtaccg catagttttt ttaattcatc agtcaaatct
    90961 gttactttgc cctgcggcgg ttctagaaca atcatttcaa aatcataacc caaatcgcgt
    91021 aagatttctt tttgtacttc ggcgtaatag cctaaacggc acggtccagt gccccctagc
    91081 atcaccacag tatcggcacc tgcttccaag ccttcaataa agttacccag ggtaatcttg
    91141 agcggaaagc aggcgaattc cggtgcattt aaaaccccta aatttattgt ctttttggta
    91201 atgggaggcg gtataacaat ttctaatccc aagtcagtta gtaaaccttc caaagcgatg
    91261 gataaggaac ccatatgtgg aaatgtaact ttcatatggc cttcctccac cgcagcatat
    91321 caacaaaagc ctctaaccgc gtgaccatcc cagcttcacc ggtatgctca tcaagtgtca
    91381 acaggataac tggcacggta cgctggcgct tatagtaacg ttctatcagc tcacaggtca
    91441 gcgaatctgg gccgcagcca aaggctgcta ctaaaaccaa gcccgcaata ttctgctgct
    91501 ggagaaaatg cagccccgct cccattaaga gatgaccgaa agtccaaaac acatcctttt
    91561 ccagtgcctc cgatccctca gcaatggccg ctgcaggcag catttctgag gttacaacct
    91621 ctacatccag ttctgttaat ttattgagga tattaagact aataaacggg tcattaatgt
    91681 tataagcatg tcccagcact gctacccgtg ttctgccaga ctttggctgg gcagctttgc
    91741 ccgctaaggc tgcctgaggg gttaagcctt gctgcaattt tgcttcatac tctttttggg
    91801 ctgctgccgc ggcctgcagt gcttgccaac ccttccaagg ccgccaatca aaacgtgcgg
    91861 ccaattgttt taatcctttc aaggtctcgc gatacttctg ctgcacatta atttctacag
    91921 ttaataactc tggcaagtta gctaccgaaa aacgtgctat ttccggcaag cctaaaaatt
    91981 taggacataa atattttctt ggttccacgg aaattactcg cggggcaaag atatagtcaa
    92041 cacctttatc gcgcaggtta gctaaatggc ctaaatatat tttcacaggc agacaagcct
    92101 catcgacagc ggcacgcacc ccgcagtcta caattgcttt actagtttca tcagacaaaa
    92161 caacttctgc ccccagcatc tggaaaaaag cttcccaggc aggataatag tggtaataaa
    92221 gcaatgctcg gggaatacca acagtactac tcatttcaac accttcactt taatctttat
    92281 tttctgtttc ttgcttgttg ctaatcactg tttcggcggc attctatcct gatctgaagg
    92341 atgtaaaatt gttaaacgag tcctttcaat gggtacaggc tgccgtaaaa tagtgctggt
    92401 tatacctcgc aaattgggag gaaaaaatgg ccacagatat ggcaccccaa aggatttgtt
    92461 agtgataagt ttcagcaaga caatcgctat tcccgccaaa aaacccggga ggttaaaaag
    92521 tccagtgaca attagcaaaa ataagtgaac taaacggtta gcttgactta attcaaaact
    92581 aggcgtagca aacatgccga cggcaactaa accgccgtat aacaagactt ccggcgtaaa
    92641 caagcccaca tcaatggcaa tttgtccaat caaaagagca gcaattaaac tcaaggcagt
    92701 tgctaaagga cttggtgtat gaatgccagc aatacgaatc aagtctatac caaaatggac
    92761 aataataaat tgaataaaaa gcggtattgc ccccgcttcc ttggggccaa taaaacttaa
    92821 aaactctggt aataattcag gctgctggga aaataacaga taaagaggtg ccaagatgac
    92881 tgacatcaac acggcaagaa aacgaatcca ccgcacataa ataccggtaa agacattttg
    92941 ccgatactcc tcagggtgct gtaaatgatg aaaaagtgtc acaggtaaaa tcatagctgc
    93001 tggtgaggta tcaacaaata gtagtaagtg cccttcaaaa agatgttgtg cggccacatc
    93061 cggacgctca gtgtaacgta ctaaaggaaa aatgctccac ctgctgtctg aaataaactc
    93121 ttctactgat ttttccgcca tcggtaaacc atcaacgtcg acccgttcta gtctgtcttt
    93181 aaccattgcc actaactcgg gattggtaat atcctggatg tacatcaggg caacatctgt
    93241 tttagaacga cgaccgagac gaaaaccctc cacgcgcaaa ccggggtcac gtacgcgtcg
    93301 gcgtgttaag gccacattaa acatcatcgt ttccacaaag ccatcgcgag caccgcgtgt
    93361 taagcgttcc gtatccggtt cctcgggact ccgcgccgga taggtgcggg tatcaagcag
    93421 aatgccttct gtctccccgt ctaacaataa cagcatctgt cctgccagta tttcatctat
    93481 cagctgcggt aggctgtcgt tgctttctat ttccatatgg gggattttct gtgtcattaa
    93541 cttgtgccag ggattgggag ccagatcctc gcgtgtaagg gtgcttagct tttgtaagac
    93601 cagtactgca acctcgccat tagttaaacc atcgatatat agcaaggcac cttcttggcc
    93661 gcctagcttc aagtcctttt ttaaaacgtc tatattgata ccaatgccca acttatcttt
    93721 aaggaagtcc aggttttcct taagttgagg actgacttta tctggtggac cgggatgctt
    93781 gcgagccatg aacaccatcc ttcccttggt gttaaaaacc ctccattaaa ggagggcttg
    93841 aaaatatctt ttagctatac attgcataaa attatctctt caccccaaaa gctaatttaa
    93901 cattggcttt atactccaca atctcgccgt cattgatatg cgctgtcaag ttagaaactt
    93961 ccactccagt aatgttatct atcgttctgg cggcctctgc tactgcgttt ttgacagcat
    94021 catcaaaact cttactagac tcccctacta attccacaat tttagcaact gacatggcct
    94081 cacctcctta gttagtggct agttttcccc atttgctcag aagaaatgca ccaggatagc
    94141 ttacgttgtc agtatttgag agtgcagcct cagtggcgac aacaaaaaac ggcctcaagg
    94201 tgagaccgtc tatttagcca tttaattgtt gttttaattg ctgcaaaact ttacgttcta
    94261 aacgcgacac atgcatttgt gacaaaccca atatttctgc tacttcctgc tgcgtctttt
    94321 gtttaaaaaa tcggtacagc ataatttgtc tttcccgttc aggcaagctg gatagagctt
    94381 gactcaaggc cacacgatca actactgttt cagcttccgc tggcgttaca gctaacttgg
    94441 tttcctcact atctaaggag atagggccac gagctacatc taaagcagct aacaattctt
    94501 cgcgctccaa gtcacaaact tttgctaatt cacttaaagt cggttgccgc cccaagcgct
    94561 gttccaaagc ttctcgcact ttcttcacac gtcccgccat ttgtttcatc gcacgactat
    94621 actttacact gccttgccca cgtaaataca ttttaatttc gccggcaatt actggcaccg
    94681 cataagtggt aaaggctgtg ccgcggcttt tatcgtagtt ctttaaagcc ttgagcaaac
    94741 caatactgcc tacttggaaa agatcatcgc cctccgcata ttccccttga taacgcttta
    94801 ccaaactatg aaccaaaggt aaatgtttat taaaagaatc atccatctcg taattaggcc
    94861 tccgtgacat ccgcccggcc aaatgttttc accaaggtga ctgtagttcc tttgcctggc
    94921 tccgtgacaa catgacagga gtccataaaa ttctccataa tcgtaaaacc catcccggaa
    94981 cgttctagtt ccggttttga tgtataaagc ggctctcgag cctgctccaa gtcggctatc
    95041 cccacgccat aatcaataat tgttacctct aaacgtgaat ctaccaagat agcactaatc
    95101 acaatagtcc ccggtccatt ttcataacca tggatcacgg cattagtcac ggcttcagaa
    95161 actgctgttt taacatcgga tatttcatca atagtaggat cgacctgcgc agcaaaagca
    95221 gccacgacga cgcgtgcaaa ggcttcattt tgtgacttac tgggaatctc taatttcata
    95281 taattatcca tctgtgtccc ccctacgcgc ctgttaaggc atcctgttca ctgctgtaaa
    95341 caggaatctt ggtttgcagc cccgccatct caaaaaccct cattactcgt gaattaagac
    95401 tacaggccga gactttgccg cccttttccg aaacaatttt atagcgacct aaaatcatgc
    95461 ccaagcctga actatccata aaatttacac cataaaaatc gaagagaata ttgcgaaagt
    95521 cttcgtggtt taatttcaaa tcaatttttt tacgcatggc atcaacagca tgatgatcta
    95581 attccccaac catctgcaca attaaagtac tgcctgcctt cttgagtttt acttgcacac
    95641 aggctcctct cctttgtcga ttaattcctt cttactattc tacattaaat tggtaattcc
    95701 tgcttgcaag ccaatttccg cttttgcagt ccccacctgg tgaaaaacaa aagatcaaga
    95761 ctctgatgct tacttagcga gtgcgacaaa gaaccgtccc ctgtcgcacc ctgtcacgca
    95821 aacaagaccg gggcatttta gccacggcct tgtttgcgct gagctggctg gattttctat
    95881 ttgcggtcaa actgtgccca gtgattaatg tagcgcgtaa ggaatgtggt gagcttggcg
    95941 cgtggcactt cttctttggg cactaaagag atactgccta gttcctcacc gtcggcagat
    96001 ttaactatta acctgcccac cgcgcttttg ggggtcaggg gagccggcag cctttttggc
    96061 agctctagtt cctgttcgta gtcggcagcg ctgcctttgg gtagaagcag ggccaaatct
    96121 gctgccgcca caatatctac ggtggtggac tgccctttat ccacagccgc ggtagctagt
    96181 atttcgccgc ctttgacaat aggcacactt tggtagttgg caaaccccca gtttaataat
    96241 tcgcgagctt cctcaaacaa taccggtcgg cctgctgctt tcataatcac cgctaataaa
    96301 cgtgtctgtt cgcggccagc agtagcggct atgcagtttc ctgcttcgtc tgtaaagccg
    96361 gttttcaagc catcacaacc gtcataataa cgaactaaat tgttggagtt acttagatag
    96421 acaccttctt ctttcttaat tttacccttc aggaaatctt cgtccatcca aattgtggcc
    96481 cactcatgaa tcttggggta ctgggacagc tccagcgaca ttaaagcaat atcgaaagct
    96541 gttgtgtaat ggttatcgtc atgtaagccg tggggattaa caaaatgcgt attcttcatc
    96601 cccagttctt ttgctctagc gttcatttgt tccacaaata catctgctga acctgccaaa
    96661 aattctgcca tcgcccaggc accatcattg gcggagccta ccacgatgcc aatcattaaa
    96721 tcttcaaagg atacacggtc acctggagag agaaagatct gtgaaccgcc gtgagccgct
    96781 gctcgttcac taatggggat catctcggtt aaggtcactt tgccgctttc cagtgcttcc
    96841 atggctaaaa gcatggtcat aactttggta acactggcag ggtacagttt ttcatgggca
    96901 ttattttcat agagcacctg tccgctagcc atatccagca acaattgggc gctggattga
    96961 aactcaaatt cgatagccaa ggcaggaaga gcacttgcca aaacaagcgt tatcaccaga
    97021 accactagca gtagcttatt tctcatcata tttcctccca gtaaaaagtt ctcagtactc
    97081 ctattttctt gccacataaa aaaaatatac cgacaaaggt cggtatttac agattttctg
    97141 cagcaaagcg ctctataatt aaaggttttt ctgctggctt ttgtgcggca aactcataag
    97201 cagcaattag ttctgccgct gccgctgcta atttttcttc attactagca aacatttccg
    97261 ctaataactc accttgttgt gcataatcac caattttttt atggagttta atgccggcta
    97321 ataagtcaat gggggcatcc ttctgctggc ggcccgcgcc cagcagaact gccgtctgcc
    97381 caactttacg cgcatctact cgagtcacat agccactcgt gacagctcgc atttccctgg
    97441 catagggcgg tgtacctagt ctcgctggtt catccactac cgcagggtct cctccctgcg
    97501 ccgcaataaa gcgccgcatc atctgcagtg ccgaaccatc agccaagagg gcggttaatt
    97561 tagccgtggc cgcctggcgg gaagcagcca gcttacctgc cagcagcatc tccgcgccta
    97621 aagtcaacgc taattcagtt aaatctgctg gtccctgacc atggagagtt tcaatcgctt
    97681 ccactacttc aagatggtta cctacagcct tacccagtgg ctgctccatc gcagtgatca
    97741 aggatactgt ctttctgccc agcgctgtac ctatcgccgt catcgtccgt gccagctgcc
    97801 gcgcatttac taaatcgcgc ataaaggcac cgctgcccat tttcacatct aaaacaatac
    97861 tttgtgcccc cgaggcaatt tttttactca tcactgacga agcaatcagc ggaatactgt
    97921 ccacagttgc cgtcacgtca cgcagcgcgt ataactttcc atcagcaggt gtcatgtctg
    97981 gtgtttgccc tgtcagggca actcctagct ctcgtacctg tgccaggaat tcttcctggg
    98041 gcaaatctgt gcgaaagcct ggaatacagg atagtttatc aatggtgccg ccagtatgtc
    98101 ctaaaccgcg cccggacatt ttggcaatag tgacaccagc tgccgcggcc aaaggcagca
    98161 gtactagggt tgttttatca ccaacgcccc cagttgaatg tttatctgcc accaggcgac
    98221 ccaaaccaga aaaatcaagc acctcacctg aatgtactaa agcatctgtt aaagccactg
    98281 tttcttcgtg attcatgccc tggataaaaa tagccattaa aagtgcggaa acttgataat
    98341 caggaatttc accgctcaga taagcatgaa taaactgact aatttcactt gtggtcaggg
    98401 ctaaactatc gcgtttcttt tccataatct ctaagattct cattacactt caaccccaat
    98461 tcctgagaaa aatctttgcc cgcagccaga ggtgctgccc ctaataaaca agcaaccgtt
    98521 gctccaacat cggcaaaggt ttcgcgcaca ccaagcggcc ctggcctaac ctgtgcccca
    98581 taaactaaga gcggcacata ctcgcgtgta tgatcggtat ggtgaaaggt aggatcacaa
    98641 ccatgatctg ccaagataat cagcagatca tcagcagtca ttttgtcgct gactgccgct
    98701 aattgaccat ctacacgctc taaagcctgt gcatagccgg cagcattatt tcggtggcca
    98761 taaaccatat caaagtccac caaattaata aaaagcaatc cttgctggta gttatccagc
    98821 aatgtcagca gtgtcgacat gccatcatca ttgttcgctg tatgcactga ggcggtaata
    98881 ccccgccccg caaagacatc attaatttta ccaatggcga ctacttcctc tccggctgct
    98941 tttagcttgt ccaaaagcat ttcctgagga ggtgccaagg aataatcttt acggttggca
    99001 gtacgctgaa aattacctgg ttctccgaca aaaggtcggg caatgactcg ccctacggca
    99061 tagtcaccgc ttaaaagttc ccgggccatt tcacaccagg cataaagctg ctccaccggc
    99121 accaccgctt catgggcggc aatttgaaaa acactatcgg cagaagtata gacaataggc
    99181 tttcctgttg ccagatgctg tgcgcccagt tcggcaatga tttccgtgcc tgaggcttta
    99241 acattgccta aaaaagcacg accgatggct ttactaaaag gctgcatgat ttctggggga
    99301 aaacctgccg ggaaaacagg aaaagcttgc gacaaaataa tacccgtcat ttcccagtga
    99361 ccagtcgtcg tatctttgcc tggggagcgt tccgccattt taccataggc tgcgcgcggc
    99421 tgcccgctac aaggaactcc ggcgatttca gtgatacagc ctaaacctaa cgactgcagc
    99481 caaggcagct gtaacccacc aacagcttgg gccgtatgag ctaaggtatt actgccagca
    99541 tcaccataaa aggaggcgtc gggcaattcc cccacgccta cactatctaa aactaccatg
    99601 atcacacgtt taatcatcag cgcctgctcc ttactatgct cgtggatggg ttttattgta
    99661 aatatcccgc agtttgcgct gcgtcacctg ggtgtaaatt tgcgtcgtgg aaatatcagc
    99721 atgtcctagc atttcctgca cagaacgcaa atcagcaccg ttttccaata aatgtgtcgc
    99781 aaaagagtgc cgcatcgtat gcggtgtgat gtccttagca atccctgctt tttgggcata
    99841 cttttttaaa atcttccaga atccctgacg tgttaaacgt ttgccatgca aattgacaaa
    99901 cagagaggtt tcagaacgtt cacgcaccag cttcggtcgc cccttgttta aatatgtatt
    99961 aagatgttga atggcaaagg aacccagcgg gataattctc tcctttgagc ctttacctgt
   100021 acagcggaga aagccggcct ctaaatttat atctaggata ttcagtgaca ttaattcaga
   100081 aaccctaata cctgtagcat aaataacttc taacattgct ttatcccgga gaccgcttac
   100141 ttgcagctga tctggctgct ccaagagtaa atcaacctct tctgtggata gcacgcgggg
   100201 caattttttt tcaattttgg gtgagcttaa atccgcagca ggattctcca ccacccttcg
   100261 ttcttgaaag agaaaattat agaaagagcg tatggaagcc atattacggg atagcgtagc
   100321 agtggcccga ccactttttt gtaattccct taagtaaccc acaatattat tccgtgttac
   100381 atcttcaatt ttagttacac caaagcgctg taagtattga caaaaagtct ttaaatctcg
   100441 gttatatgct tccagggtat tttgggccaa gcctttctct acagctaaat agttaataaa
   100501 atcccgtaat gtcttctcca tctatgcgct ccttctttag cctacaactc gcatctagtc
   100561 cctatattca gaacatcatt cgtcaaaaat gtgtctaaat cctcttcttt tagaaaaatc
   100621 ataacccgtt ttggtaaaaa tcatgtaaat agtataaaaa acgtgttggc gtgggtaaac
   100681 tttccacgct ttggctgacg ttcgctgaaa ccttcagcgc agctccccgc ggctcgcgaa
   100741 agattacagc ctgcgggtgc cttaactgca gcacataaaa ctgcagcagt ggtaagacca
   100801 caagcagcag cagaaatccc cacagtaaac ttgttagttt tttcccgcga aaataaatca
   100861 tcaccgtacc tcctgcactt ttaaataatc gaagccaccg cacgcataaa aacaggcgta
   100921 atcatcgttt ctatcaaact tcccaccagc agacagccgc caaggatcaa tactaaaaca
   100981 gaatactgca tcataaagga accatacggt aaggattgtt tgcgtaaacg actgcgcagc
   101041 agtgtcaggg aagaggacat ggcaaataca ccagccacta aagtcagcgg cacatatact
   101101 atattttgcg gaactacggc tgccagagca aagagaatac ctggcaaggc actctgctgt
   101161 actagaaaac caaccgtaaa gccgatggaa aaaccacgat aaaaaataca gcccattgcc
   101221 agcaaaatgc caaaaaagag attgccgctc aaccaaataa ttagtaaaaa gatcccatgt
   101281 tgcagcagag agcgacgcaa gatcatggtt tggtttaacg gttcctgtag tgtcaggtaa
   101341 tcaagaaagt taaaaaatat ttgttttaac tccgcaatct gctctatcgt tagtaaacgc
   101401 gtggcaagtg caccggcagc aatgccaatg gtaaaaataa tcatggctaa aaaatacatg
   101461 cccttatttt cttgtaaatg ctcgctgatg ctgtattgcc ttccctgcct cactcggcct
   101521 tcccccctgt ccatctggct cagtttatgc agagaaggtg atagatatga ctggctaggc
   101581 aagacctaat tgtacggcta aaagcacagc aacaattgtt ttggcatcct ctatttcacc
   101641 gtggctaatc atctgcttgg cttcagctaa aggcagcaga aaaacctcca gaatttcatc
   101701 ctcatctggc tgcgcctcat tttgcacaga caaccctgtt gccaaataaa catagatttt
   101761 ttctgaagca aagcctggtg aagtgtaaaa agctgacaac aagcgaaagt cattagcttt
   101821 caagccaact tcctctgcca attcccgctg cgcacaggcc aaaggtgatt ctcccggctc
   101881 caatttaccg gcagggattt cccacaactc tttttctaca ggcttgcgaa attgcttgac
   101941 aaaatatgct tctttctgtt tattcagcgc taaaatggca gcagcaccgc tgtgctccac
   102001 cacttctcta gtcgcctgct taccattagc taaggctaca ttatctagat gcagcttaac
   102061 aatacgacct tcatagattg tctttctgga aatagttttt tcgctcatcc cgcacctccc
   102121 agacataatt ctaccactga ccacatataa gttacaaacc ggcaaacgtt aagcgagcta
   102181 aacttgggac aggctgaaga cagcccaagc ccagaggatc gtcctgcaga ggaggtaaaa
   102241 ttatgcaaat tatgatgcaa aggaattcat ttcatttttt tggcacgatt cgtgaattac
   102301 gcttcttttt acgcacttta cctagagatc taaccctgcg cgaatttatt gccctgcaga
   102361 ttaattaagg ctctatcaac aaactaacat gctgcgccgt ggcaacagca gccaaaaaaa
   102421 aggcgggatc atccgtcagt gttctgccca ttgtccgaca tagaggctga tcgtctgcca
   102481 aagacgccag agacaagcct tcgtagcgaa aaatgcgatg tttgctggcc aaaccactgc
   102541 tggcaatttg ctgctccagc agcttatttt cagctgcggc taacatcggt aaaggtaaat
   102601 caaccggcac caatgttgct ttagttaagg ctgtaagcgt atgatgagaa aagccctgat
   102661 gacgcgggcg acgatcggca aagcttagtc gcggcaaggc aatgggctgc ccctttaaac
   102721 tagctgcacg gttgaggtta tcggccactt ccatgccgct gaaaccgtag cgagtagcag
   102781 ttcccgccac cccagggccc atacagacca ctgtcacatc ggcagccaag acatgtttgg
   102841 ctgccagcag accgctgtaa atattaattg cttcccaatc gccgccaaag gcctgtccgg
   102901 ctgtaattac cccgcataat aactcacgtt cctttaagat acgcagcgta ttactgaaaa
   102961 aagctggcaa agcgccgccg tctgtcatca gataggctaa gcgcagggca ggcttttttg
   103021 cttttagggc aatgcagaat ggtgctaaca tactatgtaa ttcgcctgca atcaccggca
   103081 taccgactag agattctgct tctgccatca gcgcatgctg aggcgaagcc tcctcttcta
   103141 cggccaagac tcgcacttgc tgtggggtat aacgtaactt cataatatga ccagcaccct
   103201 ggctagtatg ctctggagac gctaaatttg ccagcacaaa gtggtagccg ccgctgccca
   103261 gttgtaattc tacagccata gtattgatta agacctgatc gccaggcgca gctgccccag
   103321 ttaaaggcaa atagttaatg gcttgccctg tggtcgctcc ccacttcact gccagcacct
   103381 catactgggc agattgtgac tctacagcct gcacgacggc tgcatgacga ctaaacatca
   103441 cagaacttcc tcacgttctg cagactaagg ttctgcatca gcttaactaa tccttccaca
   103501 atttattttc tcctttcgta caccccagct gatcaagaaa ggacaaaaca ggatgctccg
   103561 caatcacttg ggttaaagat attttttctg tgagcacatg caagcccact agcgcaataa
   103621 aatacactag ctgccagata gcaggagcag ttaatacgag gttaagtccc agcacaccgc
   103681 ctaaaatatt ggctccggca tcacccaaca tcccctgtcc tgacaaatcc agcggcaggt
   103741 aggccagagc ggccagcaga aagggaaata gcaggagtac tcctgtttcc acaggtacca
   103801 gccatactga taagaaagca agcaaaaaga aaacttttaa agctcgacca ggccgtaaat
   103861 ctagtaaatt taaaagattg gccgataagg ccaccaaggc agcccgccaa aaaataagca
   103921 gcacagagcc gggcaagcca tatacagccg cgagacaaac aagaaaaccc agtgctgctt
   103981 ttaaaaaacc agtggttagc tcgccatgta tgatcaagcg ctcaaaatgg ccgctaaaac
   104041 ccttcactct tttgtcgccc cagacatcat ccaccaagcc caccagcccc aggccaccag
   104101 ctgttactaa aactcgccac acagcagcag cagtgagtag ccctagctgc tgtgcccata
   104161 aggccgccac gagaaaaaca agtgggaaga cggcgcccat actttgggga atctcttgat
   104221 ccagataatt cttttcccga tgaccgacca gctttaatat ttctgaccac accgggatga
   104281 tcactaacgc tccgccaagt cctaaaatga gataaatagc cgtcgccact atgccttcac
   104341 atccctgttt gaccatagtt gctgcaatgt ccgcagaagg tgcaggaact gcttgccgcg
   104401 atgccgaaag cccgacaaat cacgtcccgt ttcccggtgc gtcatctgca ccggtatttc
   104461 ctgcaggcgg tagccgttac gtaagcattc tactgttaaa cctacttcta ccccaaaacc
   104521 agccagcggc gtgcgtaaat tatcccagac ctcacgacgc aggacccgtt gaccacagag
   104581 tggtgaaaga ggatgataac cgcagagctt acgaataccg tagatagcta aacctttgac
   104641 caggcctaac ccgccccgac gtcgagcagg cggaaaaata gcaattgtca tgtccgcctc
   104701 atctcttaaa accggctgca gcaggtaagt aaattcttta gcagactccc ctaaatcggc
   104761 atcaacaaag caaagtatct taccttgcgc taaacgcact ccctcttgca gggctccccc
   104821 tttaccgaga ttatgcggca gctgccgcac actagctccg gccagcaaag cttggaccgc
   104881 cgttaaatcg ctggacccgt catctatgac cagaatttct tcaacttcag caattttttg
   104941 cagcgccaga atagtggtgc caatgctttg tgcttcgttg taggcaggaa taatggcaga
   105001 tacctgcatt ttagcctctc cttaataaat cgcaataaac tcctcagccg cctgtttaat
   105061 accaaaatta ccgctactgc ctcgtaaaac agaaagcagt gaatattgtc caaagacagt
   105121 ttccacatta tcgatggtgg aaaccccctg ctgcttcagt tcagttaacg cagagttttt
   105181 aacattactc cactctaaac caaccagcgt tactcccgcc tgctggcaaa cttctgtcat
   105241 ctctgccagt aaactacgat caagatcttg acgctcgccc acgaggagca gtaaagtatc
   105301 tggtttagct gtcggttgta tttcaatctt gattgcttcc ttagctaaaa aatcagccag
   105361 ttgttcttcc atgacttgac tgcattgacc tgccatcgtt aaagtaccta aggcttggcc
   105421 cacattttta gtatctgaac ctaggaccgt cctatcttct atgcggatca cactgcaaag
   105481 catcgcctga gcatcttgca gcagggtcag caggttgggc ggaatactgg cagcgtgagc
   105541 aacaacggca atatttttat cgcttaacac tcccgcgaca atatttggat ataaaacttg
   105601 atgatattgg tcccataatg ataaatcctg cgtcaaagct tgcacgttag cctgcagttg
   105661 tgaacgttct tcccgcatca gctgaaaatc gcttgtcagc tgatcaataa gtaaacgctg
   105721 ctgcgttacc aacatatctt cagtgagtcc agtgccaata ataatcccga tgcctaacgc
   105781 cagaaaaaca gctaccagcg agatgatctg gtcgcgtagc ctaaacatgc ctaactcctc
   105841 cctataaggc caggcggaca cgcaaggcaa ttaaacgaaa aaggtgccgc actgttggat
   105901 tggtaatcac taaggccaat actggtatac cagcagccaa agtcagtaag actaatgaac
   105961 gcccagaaac acgaccgtta taaagctggc tcacgccacg cgcatctact aaacggtctc
   106021 ctatttttaa acgagccaaa aaggtactgg ccataccttt tctgcccttt tctaaaaaat
   106081 caatcatatt agtatgggta ccaatagcag caattagttt ggctccaagc tcataggcta
   106141 gcagcagagc aagatcttca ctagttcccg gggctttaat tactgctgcc ctcaagccaa
   106201 gatgctcgat tctggccata cctggagcac ggccatcggg ataagcatgc accactcgtt
   106261 ctttggcttt aattaatgct tcatcactaa cactatccat atctcccact actaaatctg
   106321 gagtatagcc gtactcgcac aaagcatcag caccaccgtc tacaccgacc aaagtaggct
   106381 taacctccgc aatataatgt ttaattgcct ccaaatcttc gcgataggat tgaccgcgca
   106441 ccacaaccaa agtatgtttt cccgcaaaag tagtccgcag cggcggtatc gtcaggccat
   106501 ctagaatcag tttcttttct tggcgagcat agtcaagggt gttttgcaca aaagcatcca
   106561 gtaaattgcc taaattcgct tcggctgctg ctgtttgttg tcgaataact tcttgatcaa
   106621 ctttacgtac tgtggctagt aactcctgct cacgccaaat gccatgatga tcaacggtta
   106681 agagctcacc atcatggatt ctctccatca aatcagcacc cgccagatca tagtgaggaa
   106741 tacgcgcctc gcacaatact tggggtcctt tattgggata atggccggaa atggagtcat
   106801 taacattaac caccgcacat actttatgcg ccactaaaga ttgtgctgcc aattcatcaa
   106861 gatccaggtg atcaatcagg gcaatatcac caggttctaa acgcgacacc aagattttag
   106921 ttcgcttgcc tgccttaatc ggtccccgca ggctgacagg agctctaaaa tgccatttca
   106981 tgcgcatctt tcctaacctt ctgtttttgt tattatgtaa ctcacggcga taatttaaac
   107041 ctgcgccagt tttttcatca cctgcgtgat taaaaatagc gacctgcaca gagtgcagat
   107101 cgctattttt aaactgtagt aaattaaatt cttaaattag tgcagacttc tgctggaaag
   107161 gcgcagccga tctgccacca aggcaataaa ttccgagtta gtgggcttgc ccctttctgt
   107221 gttaatcgtg taaccaaaga acttcttaat ggtgtcgata ttgttccgcg accaggccac
   107281 ctcaatggcg tggcgaattg cccgctccac acgcgaactg gtagtatcaa atttttcagc
   107341 aatgcgcgga tacaatactt tcgtcacaga gcttaataaa tctacttctt caactaccat
   107401 catgattgct tcccgtaaat actgatagcc cttaatgtgc gcaggaatac caatttcatg
   107461 aataatattg gttatatcgg cttccagact ttggcttgtc ctttttaccg tgcgggttaa
   107521 tgaactcacg gcactactag ttgattgacc gttgcctaat aaacgaattc tatctaccag
   107581 cacttccata ttaaatggtt tcaggatata atacgctgct cctaactcta ctgctttacg
   107641 ggtaatatct tcatgaccaa aagcagtaag cataattact ttaggtttat ccggcaggtg
   107701 gtttattttt tccaagacgc ctaacccatc taaatgcggc ataataatat caagcagtag
   107761 tacatcaggt gggtcttccg taactaactc cagggtttcc ctgccgttat aagcgatgcc
   107821 agctatgtcg atatctggct gctgctccag atattccact aataagtcgc tgaattctcg
   107881 gttgtcatcc gccacaaaaa ctcttaacat tctcctatcc cctcctatat cggtaaaact
   107941 gtggttgttt tcttgtagat ttattcgaca tttggataga aatacctttc tgattattac
   108001 aataattttt aaaacttttt taggcattaa aaaattatgc cgtaggggcg aactgcgttc
   108061 gcccgcttgg ttgatttcgg tgcgggcgct tgatatcggt gcgggcgcac aaagtgacgc
   108121 ccctacggtg atgtgcaaaa aatggcgacc ctacctaaaa aaaaccacgc cttttgttaa
   108181 agtaggggcg aactgcgttc gcccgcgttg cttgatatcg gtgcgggcgc ttgatatcgg
   108241 tacgggcgca caaagtgacg cccctacggt gatgtgcaaa aaatggcgtc cctacggtaa
   108301 tgatgcacaa agtaacaccc tacccaaaaa agccaacacg tctttgggta agtaggggcg
   108361 aactgcgttc gcccgcttgg ttgatttcgg tgcgggcgct tgatatcggt gcgggcgcaa
   108421 aaaatggcgc ccctacggtg atgatgaaca aaatggcgcc cctacctaaa aaaaaccaac
   108481 acgtctttgg gttaaagacg tgttggattt tagatgttgt tattatttgc ggccagcagg
   108541 ctagattcag ccaccatcca ctccaggaaa atcccgtagc ctcgggtcgg gtcattaaga
   108601 aaaacgtgag ttacagcacc tacgagcctg ccatcttgga taatgggact gccgctcatc
   108661 ccttgtacaa tgccgcctgt ggcgtccagc aggcgctcat cagtgatttc aataattatc
   108721 cctttatcgc tgggttggga ttgattataa actcgttgta tttggatgtc aaaacgttca
   108781 atggttgtcc cttccaagac ggttaagatt tgtgccgctc ctggtttaac ttgtgaggct
   108841 aaggccatcg gcaaagcctt attttcacta cctggcatcg gcgcaatgct ggagagacgc
   108901 ccaaaaatac caaattgaca gtttttttcg atactacccg ctaaatcttg atcttcctgg
   108961 aagattcccg ttttttcacc aggatagccg cgttgagcag ctttaatgtt aacaatattc
   109021 gctttgacga tttggccgtt ctttacttca atgggctgat tggtatcaac atccgttatc
   109081 acatggccta aagcaccata aatcttggtt tggggatcgt aaaacgttag ggtaccgacg
   109141 ccggccgctg aatcacgtac atacaacccg acacgcggcc gttgcgtttc ttgacaatat
   109201 gaggggttca ctttggcggt aaagcttctc tctccacgtt taatttcaaa tttaagggta
   109261 tttttgtcct tgtgctggtt aatgatttct gccacctggt tggcatcttc caactttgtg
   109321 ccttcaatag tcaaaagcac atcgccaact tcaattcctg cttcttttgc tggtgaagta
   109381 gactttccct gactttggac cagatgatgt cctaccacca agactccttc actatgcagc
   109441 ttgatgccca cggaatgccc accagggata aacttttttt ctggtaaaac gttgacggtg
   109501 agttgacgca atgggataac accgaacaac ttaaactcca agttaacaga tcccaaactc
   109561 tgtgcgctaa actgcagcgg gctgcttaaa tctagcttac agctttgcac tgataccgga
   109621 ctgccattga gggataagat gcccgcctta tcacccttga catgaatgga aagcgggctc
   109681 gctatgttta cataatactg gcagccttcc aggatgcgca attcattttg catcgctacc
   109741 cagatgcgcc agggaaaagc tgttgccagc agtaagacaa ttcctagcag cagccaggca
   109801 ggcgcttgcc gtttccaccg tcttttcacg cggttatcac cccttgtcac atcgcttgga
   109861 aaacacctcc acataaaaat tgaaagtgaa tgtaatttta agttaaccgc gcagcaatcc
   109921 ttttatacga ttctagttta cataatatat ttgtatcatc tggctttact gacaacaaaa
   109981 ttgcccaaaa taagtgtata actaggcacc gcagctaaag cttcattaat ctacctagca
   110041 aaaaatttta catacaaaaa aaacgtgctc cttttatcct gccctgagca cgtttttttt
   110101 aattattttg tttggccagc caaagcaatt attaaaaatg aaattaatta gctaacagtt
   110161 ggtgggcatg tgctaaggct gcttcgtcct cgccgcctag catgcgcgcc aattcaactt
   110221 ctctttcagc tgctgataat ttttttagtt tggtcgcggt acggttgttg ctactctctt
   110281 tataaatgag aaaatggtga tcggctgctg ccgcaatcag gggctggtga gttacacaaa
   110341 taacttgacg gtgtgccgct aactgcttaa gcttttctgc caccgcgcgc actgttaagc
   110401 cgccgatacc ggcatctatt tcatcaaaaa ttaaagtggc cacagcatct tccgccgcta
   110461 aaacgctttt tatggctaac atcacgcggg aaatttcccc gccggaagca gttttagcca
   110521 atgaacggag tggttcacca gcattagcag ccagttgaaa ttcaatttta tcacaaccgt
   110581 aggcactgta ctggtcttcc ggagtgacca caatcacaaa ctgagcgccc tgcagcgcca
   110641 aatcttgcag cgctagatta atctgctcac ccaaattaac tgccgtctcc tgccgcagcc
   110701 ttgttagttc tgctgcggct ttttgcagcg cagctttggt ctcggcaatc atggctgtca
   110761 gttcttccct ttttgcccgg cgaccagtta aaaccgctaa ctcttgctgc atttcagctg
   110821 tcaggtctgc aacttgagct aggcttggac catatttttg tttaaggtgg cgataggttt
   110881 ccagccgctc cgtaactgcc agcagctctt cttcatcaag gtggagctct tcctgataat
   110941 accttaactc ccgcgccgcc tccctaattt gctcagcggc actgctaatc tgctgcagga
   111001 tctcctgtag tgtggcatca aggtcagctg ctgctgccag ctctttttcc accagtccca
   111061 attgatctac aattgtggcg tcgaaacttc cttcacataa gacagcataa acaccagccg
   111121 tgcgctctat taattgctgg gcattagaca agcgacgcca acgcactgcc agctcttctt
   111181 cttccgccga cgttaagttt gcttcctcaa tttcagctaa ttgaaaacgc aaaaaatcag
   111241 cttgtctaag cacggcggca tcatctccgc ctagctcttg taaatctttc tgcagctgct
   111301 gcaggttttt atactgtgct gacacctgcg tacggcactg tgttaatttt tctccgccat
   111361 aagcatccag cagcgctcgt tgtgtgctga cctctagcaa tgattgctgc gtgttttggc
   111421 catgcaaatc taccagctgc cgaccataca cggccatctg tgcaagaggt tccacccggc
   111481 cgtttagccg gcaaacattg ggcccgctcc gtcgcacctc gcgactaaag attaattctt
   111541 cttccttcac tgtggcgtcc ttgctttctt cccacgtaaa aatggcctga ataagggcgc
   111601 tttcttcacc agtgcgaata gcgtcggcac tggcccgctc acctagtaat aggctcacgg
   111661 cattaagcag catagattta ccagcacctg tttcgccggt aatcacattc agaccaaaac
   111721 caggataaaa agctaggtta tcaatcagcg ccaggttgcg aacttccaac acgcgtaaca
   111781 tggcaatctt acatcctttc tggctgccaa cttattactt tagtcagttt ttgatgcaaa
   111841 agtgtgtaaa agtcggtacc ttgaacttga atcaagggcg tagtcagtgg tgctctggtc
   111901 acctgcacga tatcatcatc ctgcagggta atgcctgttt ggccgtctat cgtcaccgtt
   111961 acttctatat ctctggtcca aggacgcaca acaacacgtt ctttactatc aacaatcaca
   112021 gaacgatgat gtaaaagatg cggacaaata ggcgttataa tcatcacatc cagttctgga
   112081 ttgacaatgg ggccgcctgc agacaaagaa taacctgtag aaccagtggg ggtcgaaatg
   112141 ataatgccat cactaggata ggtttctaaa tgtttatcat taacatatgt atcaagataa
   112201 ataattcgcg aggccacacc tttgctaatc accacatcat ttaaagcagt gtactcgaca
   112261 agttggcgat ccccgcgaaa gacacgcgcc cataacatga tgcggtggtt gatacgataa
   112321 ttccctagta ataactgttg taaagcttct tccaacatgg gcggctctac ttccgctaaa
   112381 aatcccattt gccctaaatt taatcctaag agcggtaaat tggtgccaga aaactgatgt
   112441 gcagcacgta acatcgtgcc atcgccgcct aaaaccagca ctagatccac tttgccgggg
   112501 aaatctgctg tatccaggac cggtagatcc gccagcggct ctaattcatc agaggcaact
   112561 agaaacgcct gcataccatg cgcgcgacaa aagtcggcaa tacgctctac cataatttta
   112621 acattggcta gcttcttcca gttgggaacg atgcccaaaa ccttcatgtc tttcctcctg
   112681 tcaagtacga aagcggactt cctgcagagt ttctttttcc aggtgtaaaa ttaagatgcc
   112741 acgtgcccga aaagctaaga catcgcgcag taaactactg cgccataatt ttgactgcag
   112801 gcgcacttgt ttattatcgc gtctaatccg caccagataa cgctgcccat ggcgtttgac
   112861 taaataatcg ctttttaaag taaaaggatg cagctgccca tcaatctcca tgttaattgt
   112921 cagcgttggt ttgaccacca agatttcgca gccagcttct tctaatattt gtacagcttt
   112981 accgcgcgct tgtgtggttt ttttgccgcc aattcttccc gagcggcctg ccagccagcg
   113041 cgccacaatt actaaaatga ctaaaccaag caggacccag tcttttgatg ccattttgca
   113101 ctcacctgcc taagcaattt attcgtcacg attggcaggt ttcctttttt ctgttaattc
   113161 ctgatgtgct tcagccaccg tagccgcaaa atctatttcc ttagcaatgg cagcagcgtc
   113221 tgttgccgcc aataagtgca gcaaatattc aatatttcct tctggaccgc gaataggtga
   113281 ataactgacg cccgccacgt catagttcaa ctctacagcg agtgaggcca ctttttgcag
   113341 cacagcaaga tgatcggcag ggtctcgcac gacacctttt ttacctacac gacccttacc
   113401 aacctcaaat tgtggtttaa ctaaggccac tatttcgggt acctgcagcc gctgtagagt
   113461 aggcagcacc agcgccaagg aaataaaggc cacatcgatg ctggcaaact gcaccgcttc
   113521 agctagggcc tcagcttcca gatagcggat attagttctt tccaggttga ccacccggct
   113581 atcttggcgc aaacgccagt ctagctgccc gtagccgaca tccacactat aaactttttc
   113641 ggcgccctgc tgcagcatac aatctgtaaa accaccagtt gaagcaccca cgtccatagc
   113701 cgtgcgtccc gctagctcaa tttggaaggc agcgagagct ttttccaatt ttagcccgcc
   113761 gcgcgagaca taacgcggca attccttaac cgtcaccgca acattagctg ttacctgggt
   113821 accaggttta tctacctgtt gcccatctac cgacaccaga cccgccatca cggcggcacg
   113881 tgccgccgag cgactgacaa ataaagcacg ttctgccaac agagtatcta atcgtttacg
   113941 ctctttaccc atgagatctt ccctttgctg ctagctaaac ccagacaagc agcagcgata
   114001 ttttctacag ttaagcctac atctttaagc agtaagttac gggccccatg ctgtacaaac
   114061 tgatccggta tgcccaaccg tttaaccggc agcgtctgta aaccattgtc ctccaaatac
   114121 tctagcacag ccgaacccaa accgccggcc aaaacatttt cctcaaccgt gaccagacca
   114181 taacggctgt tcagggcggc acgcaccact agctctgtat ctagtggttt aacaaaaatt
   114241 aaatcaatca ccgctacaga caggccattt ttctctaact gggctgccgc cgccagggcc
   114301 ggcgcgacca ttgttcctac cgccaaaatg gtgacatcgc gtccctgccg cagcatctca
   114361 ccacgccccc aaggcaggca gcgcggcgtt tctaatgctg ctccctgccc cttaccgcgc
   114421 ggataacgaa tcgccaccgg actgttatgt tttagtccgg taaataacgc atgttgtaga
   114481 taattttcat cgcgtggaca gataattgac aggttaggca gagggcgcag caaggaaatg
   114541 tcaaacacac catgatgcgt ttccccatcc tcacctacta agccagctcg atctacagca
   114601 aagactacag gcaattcctg catacatacg tcatgcagca cttggtcata agcccgttgt
   114661 aaaaacgtag aataaatcgc caccactggc ttaaaaccct tgcgggccag accggcagca
   114721 aaggtaacgg catgctgctc cgcaatgccc acatcaaaga aacgctgagg aaaagtctgc
   114781 gcaaaaccgc tcaaccctgt gcctgaagtc atagctgccg tgatcgcgat cagcttttgc
   114841 tgctcacgtc ccaactgcac catagtatca ccgaaaacgt cagtgtagga aggcacactg
   114901 cctctcgtcc caggctgggg cggcgactgt ggatcaaagg ggccgacacc atgataaaaa
   114961 tcagggttat tttccgtaaa gtcatatcct ttgccctttt tcgttaagac atgaatgagg
   115021 atgggacctt tcaacttttt ggcaccccgc atgacgccct gcatggcctc cagattatgc
   115081 ccgttaactg gccctaaata ggtaaagcct agttcttcaa aaagcatgcc tggtacaata
   115141 aggcttttta cagcatcttt tatgcggtcc actgatttaa tcatttttcc gccaatggca
   115201 ggaatcttca ggagaagttc ctggaaatcc tctttaaaac gaaaatattt ggggtctgta
   115261 cgaatacgac ccaaataagc tgccaagcca ccaacattgt gggaaataga catttcatta
   115321 tcgtttaaga cgacaatcaa atttgttccc atgtcaccag catgattaag agcttcaaaa
   115381 gccatcccgc cgcccatcgc cccatcgcca atcaccgcca gcacatgatt atcttttcct
   115441 aataaatcgc gggcttgtgc cacacctaag gctgccgaga tggaagtaga gctgtgcccc
   115501 gtctgaaaaa tatcatgttc gctttcttta attttcggaa agccgctcat tcccccgtac
   115561 tgccttaagc ttgcaaaacg atcagcacgg ccagttaata ttttatggac gtaactttgg
   115621 tgacccacat cccaaataat ttcgtcagtc ggactatcaa aagtagtatg tatcgccagt
   115681 gttaactcca ctacaccaag attcggggca agatgccctc ccgttttaga cacattcgcc
   115741 aagagaaaag cacgtatctc accggctaaa gctgttaatt ccttgttact aagttttttc
   115801 aagtcagccg gacctgtgat ggtcggcagc aacttactca tgttgtttac ctcactttcg
   115861 cttacgagga agcgctcgtc caaagaatat ttttcccggt aatactgttt ctattttagt
   115921 taaaacatta gcaaatagta agataattac cgtcgaatgt tctatcgcta ctgacttacc
   115981 aagagccttg cctccaacgg tgagcgctga aataattcct gtcgcggcaa tactgaccac
   116041 gggattagat tcggccccaa atattcttac caccagtaac gcacttaaaa caccactgat
   116101 gatgcccgcg atatccccca ccacatcatt gcagaaatta gaaaaacgat cagcattacg
   116161 aattaaatat aatgcttcac gcgccccagg ggttttttta gctgctttgg ctaaaatcgg
   116221 cacttcatca gcagccgtcg cagcaatacc gatcatatca aatagtatac cggt
//
LOCUS       DASRMS010000184         1243 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_990899, whole genome shotgun sequence.
ACCESSION   DASRMS010000184 DASRMS010000000
VERSION     DASRMS010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990899"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..451
                     /locus_tag="VFC74_08620"
     CDS             <1..451
                     /locus_tag="VFC74_08620"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HZK25438.1"
                     /translation="SQHALKSNEFIIIAPTSKGLHHGKSKVWPEYEKLTQSLQRQGHR
                     VIMCPPPNETTQAKANAISAELLPPLALGPYTALLKEAALVICNDSGVSHLSAVATAK
                     QITLIGVSEIEHTGPWSTSALILGEIGHWPTQDEVLAAAEKVLRGSD"
     gene            complement(448..>1243)
                     /locus_tag="VFC74_08625"
     CDS             complement(448..>1243)
                     /locus_tag="VFC74_08625"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HZK25439.1"
                     /translation="SRDMAEFPSFFSSYLVSLIVPQNLALFLLVLAVVLFIIRFKRLA
                     RLLLLSAALWLGLWSLPITSIKVGSFLEQQYAISSFEQLPKADAIVVLGGNTAANRLN
                     WFEEEVDRNSTHRRLDTAEALYVAGKAPVIIVSGGANEGPVSEARGMEASLRSLGVPA
                     EAIIREDRSQTTWENALYTKRTIEEQGIGSVLVVTSALHMPRAMGVFESLGIKAYAAQ
                     NPPQIQVPQNKPDFNPYLPNMRALAASRSIIKEYLGYWVYQFRGWI"
BASE COUNT          361 a          316 c          260 g          306 t
ORIGIN      
        1 gtctcagcat gctttaaaaa gcaatgagtt tattattatc gcacctacct ccaagggtct
       61 gcatcatggc aaaagcaaag tttggccaga atatgaaaaa ctcacccaat ccttgcaacg
      121 ccaaggtcac cgagtcatca tgtgcccacc gcccaatgaa actactcagg ccaaggctaa
      181 tgctatttca gctgaactac tgccgcctct ggctttaggg ccttataccg cgctacttaa
      241 agaagccgct ttagtcatct gcaatgactc cggcgtatca catcttagcg cagtagccac
      301 tgctaaacaa atcaccttaa ttggcgtgag cgaaattgag cataccggac cttggtctac
      361 tagtgcctta atactcggag aaataggcca ttggcctacg caggacgagg tgttagcagc
      421 tgctgaaaaa gtgttacgag gcagcgatta aatccagcct ctgaactgat acacccaata
      481 gcctaaatac tctttaataa ttgaacggct cgctgctaat gcacgcatat ttggcaaata
      541 gggattaaag tcaggcttat tttgtggcac ttgaatctgc ggcgggtttt gtgctgcata
      601 ggccttaatg cctagacttt caaagacccc cattgctcgg ggcatgtgca gtgcagaggt
      661 taccactaat accgagccta taccctgctc ttctatagtg cgtttggtat atagagcatt
      721 ttcccaagtt gtttgactac gatcctcgcg gataatggct tcagcaggca cacccaagct
      781 gcgtagacta gcctccatac cgcgtgcttc actaactgga ccttcgttag ccccacccga
      841 gacaataatc accggcgctt tgccagccac gtaaagtgct tctgcggtat ctaaacgacg
      901 gtgtgtagag tttctatcga cttcctcttc aaaccaattc agtctattgg ctgccgtatt
      961 gccacccagc acgacaatcg catcagcttt tggcaattgt tcaaaactac tgatggcata
     1021 ttgttgctct aaaaaagaac ctactttaat agaggtaatg ggtaaggacc acaaacctag
     1081 ccaaagagcc gcactcagta gcagcaatct agctaaacgc ttaaaacgaa taataaaaag
     1141 caccacagcc agcactaata aaaacaatgc caagttctgt gggacaatca gtgaaacaag
     1201 gtagctacta aaaaaagaag gaaattctgc catatccctg gag
//
LOCUS       DASRMS010000185        18554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_388003, whole genome shotgun sequence.
ACCESSION   DASRMS010000185 DASRMS010000000
VERSION     DASRMS010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 18554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18554
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388003"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1194
                     /gene="uppP"
                     /locus_tag="VFC74_08630"
     CDS             406..1194
                     /gene="uppP"
                     /locus_tag="VFC74_08630"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HZK25440.1"
                     /translation="MGSIEAIILGVIQGLTEFLPVSSSGHLVIFQDILGVQEAGITFE
                     VMLHFGTLLSVIWVFGGDILRLATSFRHEKQERNFVIMLLFGIIPTGIMGVLLKDLFE
                     RFYNLPILVGIMLLITGVILFTLSRLAPGRKNELTMTKKDALLISVAQGIAIIPGISR
                     SGSTITSALWLGLDQETAVRFSFLVSIPVILGATLLELKDLFEIGFAGFNNNMIIGTA
                     ASFFAGIFAIRIFVNILQKGKFHYFAYYCWFAGVVTILYKLLVS"
     gene            1207..3537
                     /locus_tag="VFC74_08635"
     CDS             1207..3537
                     /locus_tag="VFC74_08635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HZK25441.1"
                     /translation="MAIHDIRDEIRQQVKAVLIIALAAFAFAALLFPRQTGEVGMFFN
                     NVLRMLTGDLSIYVPVVLVINNLLKIFPWQVPNLKQRTAGIVLFFIILLVYAHLQVMA
                     AEINLVMDLGIFNASLRLGLQQQGGGVLGAILAVILYFLFKDLGSRIILLALAVIAFI
                     LVTNISLSQLLQTASRCLLFVGRSILQALKAVAGFFRFIFAQAPTEGLEVEDVELEEF
                     PALLKKKRQQSPAKAGDEAAPLSTNNQSEVAATVTPSVMESIQATLIPDTPEMTEAIG
                     AAQQLELFAAGDKPLTISIDSVHGAYHLPPLKLLPKLPKHKDPQSQKNLVERAKVLER
                     TLDSFGVKAHVTDAQCGPTVTRFELQPEVGVKVSKIVSLADDIALNLAAADVRIEAPI
                     PGKAAIGIEVPNKVIAPVYLREVLEDEKFQNATSVLTIALGKDITGNSIVTDLMKMPH
                     LLIAGSTGSGKSVCINVLIASILFNARPDEVKFVMIDPKVVELSTFNGIPHLLMPVVT
                     DAKRASLALKNMIKEMTRRYELFARESVRDINTFNNKKRQQNEETELLPYVVVIIDEL
                     ADLMMVAAADVEDSIARLAQMSRAAGIHLVIATQRPSVDVITGVIKANITSRIAFAVS
                     SQADSRTILDMGGAEKLLGRGDALFHPIGLPKPFRIQGAFINERELNNLLEFIKKQGE
                     PDFDEQLLPEEEADEFFYEEDELFPDAVMLFAEAGTASISLLQRRLRIGYTRAARLVD
                     DMESRGFIGRFEGSKAREVLVTPEQVRKLLFERDQS"
     gene            3617..4420
                     /locus_tag="VFC74_08640"
     CDS             3617..4420
                     /locus_tag="VFC74_08640"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RodZ domain-containing protein"
                     /protein_id="HZK25442.1"
                     /translation="MHHIGQQLKEARDAKGLTLKDVTERTMIPIKYLTALETEQFTLF
                     PGEVYFKGALRKYAAELGLDAQALINSYNAEQEETADPEHQKTTLAASKPPRQTPKFD
                     FTAQWRKLQLKRFLPIVAIVLLLLAGSFLLRKLMTKEPPQVDPPPLSTGQDEEQNDEQ
                     TETNSPGEPAVLVQSDTSTEEVHFMVSNAAELTAELSFSSPCWIRAFSDGKRSLDGTY
                     RPGQPQVARASEEISIRLGNPPAVTLAINGQAVQLPETKYAYTITISKK"
     gene            4561..5895
                     /gene="rimO"
                     /locus_tag="VFC74_08645"
     CDS             4561..5895
                     /gene="rimO"
                     /locus_tag="VFC74_08645"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HZK25443.1"
                     /translation="MSGLKIALVSLGCAKNLVDSEIMLAHLQEEEFDLITNPAEAEAV
                     IINTCGFIESAKQESINKILELAQLKKKKCQVLLAAGCLAQRYGEELLIAIPELDGIL
                     GTNDLQAAAAAIRRTLAGERVSFLQGEYDAPEDAPRILATPTHTAYLKIAEGCDNLCT
                     YCAIPAIRGPYRSRSRIAVVAEAKQLAQNGVRELNIIAQDITPYGLDSSGELELPILL
                     EELAQLTGIAWIRLLYAYPERLDPRTIETMARLTQVCNYLDLPLQHASDRILKQMGRK
                     FTAAKMMQLLTQLRQMIPNITLRSSFIVGFPGETEADFQELLLFLRVAQLDRIGFFAY
                     SQEEGTPAARLPQQISAQVKAARLAEAIAVQSEIITAKQEALVGQSFMAIIDGRSAQE
                     PQTLLLRTPMQAPEVDGYVRVANCDAAPGTMLPVTITGYDGYDLLGAVELSH"
     gene            6088..6621
                     /locus_tag="VFC74_08650"
     CDS             6088..6621
                     /locus_tag="VFC74_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25444.1"
                     /translation="MKNFKWQVAVCAFVLTLGLAVGAVMLRQRQVLDEPLRERLGSLA
                     AVETVELDQEEEKFVVRVKLAEVKDLALTYRALEAELNQLLAEDSYRLVLVDERNSEL
                     TEALFTVSLALYEGEQRGNFTEMSKAIAATLEELQVAEHRLTVDHEHIYLQMKNGDAY
                     LYEVVGREPWRREGERV"
     gene            6618..8105
                     /locus_tag="VFC74_08655"
     CDS             6618..8105
                     /locus_tag="VFC74_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HZK25445.1"
                     /translation="MIMEIAAGAALAIFVVLAWQGVNIAPAVVLAVMFALLYYFAGMR
                     SGGKKQFAVVEGSRNTGLVKFDQIGGQRTAKKELLEALDFIRHPERIANMGIRPLKGI
                     LLAGPPGTGKTLLAKAAAAYTDSVFLVASGSEFIELYAGVGAQRVRQIFSKARSTALK
                     EKKNSAIIFIDEIEILGGKRGSHSSHHEYDQTLNQLLVEMDGLAVEERVRTLLIGATN
                     RADLLDSALMRPGRFDRVVQVDLPDLEARQEIIKIHVGNKPLAANVDLEQLARESFGF
                     SGAHLESLANEAAILAMREQQPEISTRHFTDAIDKVMMGEKLDRKPDQDELWRVAVHE
                     IGHALISELQRPGSVSNITTTPRGKALGYMRQSPQKDSYLYTQEYLEGQIAVMVAGSV
                     AEEVVLGNRSTGAANDFQQAVKAAKQIIAAGMSSLGIVSLEDLPQDLLHQEIQQIIAQ
                     QKTEVQAFLASRKGLVSEIAEILVHDEKVTGELLRSRLEEDQMAV"
     gene            complement(8212..8877)
                     /locus_tag="VFC74_08660"
     CDS             complement(8212..8877)
                     /locus_tag="VFC74_08660"
                     /inference="COORDINATES: protein motif:HMM:NF024067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3786 domain-containing protein"
                     /protein_id="HZK25446.1"
                     /translation="MQKTAIPLAHDLNPQNYQIALDVARERFAANQPEKMAELSGCIY
                     NHQCSYFTVCCLNHPFTITYPLGLVTYKDSDLQPPFALQLIMINYLARADGIPLAYNY
                     IPYRHLEGGQTYYGAFVKTAIEPLSKVFGQQPELLQTAAQPFGGVPLTGISGNAVLIY
                     LLPRVPLLFQIWAGDEEDGLPAQANILFDSTANHYLHTEDLGACDLVSRLIMAQVKNS
                     SNR"
     gene            8977..9552
                     /gene="yedF"
                     /locus_tag="VFC74_08665"
     CDS             8977..9552
                     /gene="yedF"
                     /locus_tag="VFC74_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR03527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HZK25447.1"
                     /translation="MRQDKLDCRGFACPQPVVETKKKLAELSVGSLNVLVDNETAKIN
                     ILKLASTLGLAAAAKEEADYYSITLVKEEQAAARIAESGKNILITAATLGHGNDDLGT
                     ILMKSFFYALAESTKLPKALYLLNGGVKLACSGSPVLDSLTKLQALGVEIFSCGLCLD
                     YLQLTAQLQVGEITNMYAIVEQITSGTVIEL"
     gene            9619..10125
                     /gene="ruvC"
                     /locus_tag="VFC74_08670"
     CDS             9619..10125
                     /gene="ruvC"
                     /locus_tag="VFC74_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006522417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HZK25448.1"
                     /translation="MRILGIDPGIAITGFGVIETDGQRSSALAYGCIRTAASLSMPER
                     LEQVYRELQELIARHQPQEAAIEKLFFAKNANSAMQVGEARGVAVLTARIAGLQVFEY
                     TPLQVKQAVVGYGRAEKNQVQQMMKMLLGLDQLPRPDDAADGLAIALCHAHSGAGRAA
                     LQRRKNYV"
     gene            10118..10729
                     /gene="ruvA"
                     /locus_tag="VFC74_08675"
     CDS             10118..10729
                     /gene="ruvA"
                     /locus_tag="VFC74_08675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HZK25449.1"
                     /translation="MFNYLRGTLIDKEKETIVLENSGIGWQITVPLTVQAQLASVGQE
                     IKLYTYLAVREDALQLYGFSQREELTLFKLLISVSGVGPKAALAVLSTLSAGEFYWAI
                     MHENIKALTRVPGIGPKSARRLIVELKEKATNIAMSRGEAQHPAAGQAAVAMDAYSEA
                     LAALLSLGYQGTEAQSALNEVPEREQLATEELLRKALAYLGTH"
     gene            10740..11762
                     /gene="ruvB"
                     /locus_tag="VFC74_08680"
     CDS             10740..11762
                     /gene="ruvB"
                     /locus_tag="VFC74_08680"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HZK25450.1"
                     /translation="MEERIISAQRSNEDDTPDNTLRPRTLADYIGQQKLKDNLRVFIE
                     AARARGDSLDHVLLYGPPGLGKTTLSQIIATELGVNLRSTSGPAIERPGDLAAILTNL
                     APYDVLFIDEIHRLNRTVEEVLYPAMEDFALDIIIGKGPSARSLRIDLAPFTLIGATT
                     RAGALSSPLRDRFGVVARLEFYQEQELQIIVRRAAQILQVKIDEEGALTIAQSARGTP
                     RVANRLLKRVRDFAQVKADNFINAEVAKQSLRLLQVDELGLDEVDRHLLRTMVEKFNG
                     GPVGLDTLAAAIAEESDTIEDVYEPYLMQIGFLQRTPRGRVVTARACRHLGLTYTENK
                     EQNTLW"
     gene            11772..12320
                     /locus_tag="VFC74_08685"
     CDS             11772..12320
                     /locus_tag="VFC74_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HZK25451.1"
                     /translation="MELFLHMCCAPCSTYSIKHFRALGYDIHGYFYNPNIHPYKEFQR
                     RLETLEAYCQSVNVPLTVQRNYHLELFLQHAVSNLDDRCRECYTLRLFEAARRAKSLG
                     FTKFSTTLAISPYQDHQLLQEIGQTAAQQHGVEFIYEDLRPGYRESINISKAAGLYRQ
                     AYCGCIFSEKDRYYKGDQLEDK"
     gene            12410..13435
                     /gene="queA"
                     /locus_tag="VFC74_08690"
     CDS             12410..13435
                     /gene="queA"
                     /locus_tag="VFC74_08690"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008704676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HZK25452.1"
                     /translation="MFLADFDYELPENLIAQVPLAQREASRLLVLHKANGKCEHQQFT
                     DLKHYLRAGDALVLNNTKVLPARLLGHKRQGGALVEILLLKQRDAHTWETIVRPGKRL
                     RPGAEVIFGQGELKAQILEILPDGGRLVSFTFAGTFQEILDRLGEMPLPPYIKEKLTD
                     KERYQTVYAQEEGSAAAPTAGLHFTPDLLQQIAESGVSLVYLTLHVGLGTFRPVKTER
                     IEEHQMHSEFYQLPAEAADTLNQCKQRGGRIFAVGTTTCRTLETVTSADGIIYPGSGW
                     TDIFIYPGYRFRAIDALLTNFHLPKSTLLMLIAAFAGRENVLQAYTQAVQAEYRFFSF
                     GDAMLIL"
     gene            13484..14608
                     /gene="tgt"
                     /locus_tag="VFC74_08695"
     CDS             13484..14608
                     /gene="tgt"
                     /locus_tag="VFC74_08695"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HZK25453.1"
                     /translation="MAVNYTLIKECPSTGARAGILHTPHGDIPTPVFMPVGTQATVKA
                     MSPEELQEIGARIILSNTYHLYLRPGHELVREAGGLHRFMNWDRAILTDSGGFQVFSL
                     GALRKIKEEGVTFRSHLDGSEHIFSPEKAVEIQEALGSDIAMVFDECAPYPAERDYIE
                     DSLARTTRWAERCLKAHTREEQALFGIVQGGMYADLRQESARQLVALDFPGYGIGGLS
                     VGEPAALMYEMLDETVPILPANKPRYLMGVGTADYLIEGVKRGVDMFDCVLPTRIARN
                     GTIMTRDGYLTVRNAPYAKDYGPLEEGCNCYACRNYSRAYIRHLIKTNEILGLRLTSY
                     HNLYVLVHFMARIRDSILNGTFPDVYKEFIARFGQEKEEK"
     gene            14608..14868
                     /gene="yajC"
                     /locus_tag="VFC74_08700"
     CDS             14608..14868
                     /gene="yajC"
                     /locus_tag="VFC74_08700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HZK25454.1"
                     /translation="MDTLKLLAPMLIFFALMYFLTIRPQQQQQKHRGSMLSNLSVGNK
                     IRTIGGIFGTIEKINDDELTVRVADSVSLRMARFAVESIIKE"
     gene            14941..15339
                     /locus_tag="VFC74_08705"
     CDS             14941..15339
                     /locus_tag="VFC74_08705"
                     /inference="COORDINATES: protein motif:HMM:TIGR04086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04086 family membrane protein"
                     /protein_id="HZK25455.1"
                     /translation="MPRKNTAAMQIRPPTAILVMGKGVLYAYFFSLVVFLVFSMLIQY
                     TSLTEAILPYTAYATSLVAIFIGAAYVTRRLQQKGWLNGGITGLLYLVGLIIIAMVVL
                     PDFSLNASYFSKVVLAFATGAAGGIFGINT"
     gene            complement(15372..15935)
                     /locus_tag="VFC74_08710"
     CDS             complement(15372..15935)
                     /locus_tag="VFC74_08710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25456.1"
                     /translation="MMLVNTCKQKVLFSLLLLVCLLTAVYLRNGSTLLSLGLYLLSYT
                     GREYAAGSSKTQPRSQWYLAYLLVGYLVYSILGFLLVLASLKNFGRLNTSGIEVTITS
                     VILVITTISMVAARRQQQVGWRRFSPLIFILLMLGAQLLALHGSPLLFSFARLGVSVL
                     ILRSGFTHCYLGLNHFLQPKNSHLSRS"
     gene            16273..17637
                     /locus_tag="VFC74_08715"
     CDS             16273..17637
                     /locus_tag="VFC74_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25457.1"
                     /translation="MTSDINKLYNERLGRYQAAIALEPTDRIPTAFATTYFAEKQSGY
                     SYQQIMYDFQIWENMEVGFAKKYPDVDIFRTNLGWAATWDITNHQLWRVPGRDISPNS
                     LQQFNEQEWMKADEYRRFIDDPIGYRMDVYLPRILGEYQERGSIRSYMAFLKAGMAGS
                     IFGAAAAKRVERLKNEVGIPQGTVGGFSAPFDVLSDAYRGFYGITRDMFRQPDKVLEA
                     CEHILEQQLSNAWRRADPLKRFPIFIATHKPCFLSPKQFDTFYWPTFYKGVMKLIEAG
                     WKFRIFLEGDWGPHWHHMAEFPKGTVICDIDNEADIFDAKEKFGHQQCITGGIPTDML
                     ILGKPEDIRARVKLLCETIGKDGGWIPNGGGHIPEDTKPENFQELLDAVMEYGKYSDG
                     PAPEPKIGEPGTNGVVFPEPGIVTPWEEIKKENKWKIPGDEKIIKDWWEQLESMAYGW
                     ICSQ"
     gene            17655..18290
                     /locus_tag="VFC74_08720"
     CDS             17655..18290
                     /locus_tag="VFC74_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823746.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25458.1"
                     /translation="MTKLAVALRDLNEDLVYQLVAEKLSQGISPTEIIKECNVGIAAV
                     GDMFASGEYYLTELMFSAEIMQGVMAKLEPLIASSGEAEQTLGTFVIGTVSGDIHDIG
                     KNIVVNLMRSHGFKVIDLGVDVPAERFVEMVRESGTKILGLSALLNSTYPEMKSVIEA
                     LKEAGLKEQVKVIIGGTITSEAVREFTGADAFATDAQKGVSFAKSVYGINN"
BASE COUNT         5278 a         3724 c         4391 g         5161 t
ORIGIN      
        1 tctattctat ctaatccaca gctcagaacc gcagctaaac atttacttac gaacacggta
       61 gtgttatatt attattatgt ttttcacatc tcgcagcgta ttcctgctaa atattgctat
      121 gctgctatgc taccatagca actagataag gtttgcactt ttcaagtaga gatgttatat
      181 ccgactacca ctgtatgcgt tatgaaaaag cctgttgtag catggactac actgaaaaaa
      241 ggaggattta agaccttttt agcgaagtga tacgaaaatg gttctcatgt tcaactagcg
      301 ggacaacgat cagcaaagca tcgctgatcc tagtttgcag cgacttctac tgaattaaac
      361 aagcggacta acaaattgat attgaattcc aagcaggagg tttgtatggg aagcatagaa
      421 gcaattatac ttggtgtcat ccaaggtctg acggaatttt taccagtgtc gagttcagga
      481 catttagtga tttttcaaga tattctaggc gtccaggaag ctggaatcac ctttgaagtg
      541 atgctccact tcgggacgct tttatcggtc atttgggtat tcggtggtga tatcctccgt
      601 ttagcaacta gctttagaca tgaaaaacag gaaagaaatt ttgttattat gttattattt
      661 ggtatcatcc ctactggtat catgggcgta ctgctaaaag atttattcga gcgcttttat
      721 aatctgccga tccttgttgg catcatgtta cttattaccg gtgttatttt gtttacactt
      781 tcccgtttag cacctggtcg taaaaacgaa cttactatga ctaaaaaaga tgcgctgttg
      841 attagcgtag cgcagggaat tgccattatt cctggtatct cacgctctgg ctcaacaatt
      901 acctctgctt tatggctggg tttagaccag gaaacagccg ttagattttc ttttctagtt
      961 tccatcccgg taattttggg tgccacatta ttagagttaa aagacttatt tgaaattggc
     1021 tttgccgggt ttaataataa tatgatcata gggacagcag cttccttttt cgccgggatt
     1081 tttgccattc gtatcttcgt aaacatcctg caaaaaggaa agttccatta ttttgcctac
     1141 tattgttggt ttgccggcgt tgtcaccatt ctctataaac tattagtttc ttaagcaggt
     1201 ggataaatgg cgattcatga cattcgagat gaaattagac aacaagtaaa agcagtcctc
     1261 attatcgcct tggcagcgtt tgcttttgcc gctttgctct tccctcggca aaccggtgaa
     1321 gttggcatgt tttttaataa tgtattacgc atgctgacag gtgacttatc aatttatgtg
     1381 cctgttgtct tggtgattaa taatttgtta aaaatatttc cttggcaagt acccaacctc
     1441 aagcagcgta ccgctggtat cgttttgttt tttattattc tactagtcta tgctcatttg
     1501 caagtaatgg ctgcagaaat aaacctggta atggatcttg gtatttttaa tgctagcctc
     1561 cgtttgggtc tgcagcagca gggaggcggc gtactgggag caatcctggc agttatcctt
     1621 tattttcttt ttaaggatct aggcagccgg atcattttac ttgctctagc cgtaattgcc
     1681 tttattttag tgactaatat ttctctgtca caacttttgc agacggcgag ccgctgtttg
     1741 ctctttgttg gtcgcagcat cttgcaggcg cttaaagctg ttgctggttt tttccgtttt
     1801 atctttgctc aagccccgac agaggggctg gaagtggagg acgtggaact agaagaattt
     1861 cctgcgcttt taaagaaaaa acgccagcag tcacctgcaa aagccggcga tgaagcggca
     1921 cctctgagta caaacaatca gtctgaagta gcggcgacag taacaccttc agttatggaa
     1981 tcaatacaag caacgcttat cccggacact ccagaaatga cagaagccat cggggcagca
     2041 cagcagctag aactttttgc tgcaggggac aagcctctta ctatcagtat cgattcggta
     2101 catggagctt atcatttacc accgctgaag ttgctgccta aattgcccaa gcataaagac
     2161 cctcaatctc agaaaaactt ggtagaacgg gccaaagtct tggaaagaac cttagacagt
     2221 tttggtgtta aagcccatgt tacggatgcg caatgcggcc ccacagtcac gcgctttgaa
     2281 ttgcagccag aagtgggagt taaagttagt aaaattgttt ctctggctga tgatatagcg
     2341 cttaatttag cagcggctga tgtacgaatt gaggcgccaa ttcctggtaa agcagctatt
     2401 gggattgaag ttcccaataa ggttattgca cctgtctatt tacgggaagt attagaggac
     2461 gaaaaatttc aaaatgccac atctgtatta acaatagcct tgggaaaaga tattactggc
     2521 aactccattg ttaccgattt aatgaaaatg ccccatttgc tgattgccgg gtcaactggt
     2581 tctggcaaga gtgtttgtat taacgtgcta atcgccagta ttctctttaa tgcccgtcct
     2641 gatgaagtaa aatttgtaat gattgaccct aaagtggtgg aattaagcac cttcaatggt
     2701 attccacatt tactaatgcc cgtggtcaca gatgccaaac gcgcttccct ggcacttaaa
     2761 aacatgataa aggaaatgac acgacgctat gaactgtttg ctcgggaaag tgtgcgtgat
     2821 attaatactt ttaataataa aaagcgccaa caaaatgaag agactgaact tttaccatat
     2881 gtcgttgtca ttattgatga attagctgac ttgatgatgg ttgccgctgc cgatgtggaa
     2941 gattccattg cgcgtttagc acaaatgtcc cgtgcagccg gaattcatct ggttatcgct
     3001 acacagcgac cctcagtaga tgtgattact ggtgtaatta aggctaatat tacctcacgc
     3061 attgcttttg cggtgtcctc tcaagctgat tcacgcacca ttcttgatat ggggggagca
     3121 gaaaaattgc tcggccgtgg tgatgcctta tttcacccta ttggtttacc taaaccattt
     3181 cggattcaag gagcgtttat taacgaacgt gaattaaaca atctcctgga atttataaaa
     3241 aaacagggcg aaccagattt tgatgagcag ctcttgcccg aagaagaagc agatgagttc
     3301 ttttatgaag aggacgagct ctttccagat gccgtgatgc tgtttgcgga agcagggacg
     3361 gcgtctatct ctttactaca aagacgttta cgtattggtt atacccgcgc agctcgttta
     3421 gtagacgaca tggagagtcg tggctttatt gggcgatttg aaggaagcaa ggcgcgagaa
     3481 gtattagtca caccggagca agtacgaaaa cttctctttg aacgtgacca aagttaattt
     3541 tacgctaaaa agagcaggtc tagactaaaa agcttaactt ataagtgcag acaacaaatg
     3601 agaatggggt gaggagttgc atcacatagg tcagcaacta aaagaggcac gcgatgcaaa
     3661 aggtctaaca cttaaagatg tcacagagcg gacaatgata cccataaaat acctaacagc
     3721 gctagaaaca gaacaattta cccttttccc aggagaagtc tactttaaag gggcgctgcg
     3781 taaatatgct gcagagttag gtttggatgc tcaagccctg atcaacagct ataatgccga
     3841 gcaggaggaa accgcagatc ctgagcacca aaaaacaacc ctggcagcga gtaagccgcc
     3901 acggcagacg cccaaatttg attttacagc tcaatggcga aaacttcagt taaagcgttt
     3961 cctacccatt gtagcaattg ttttattgct gttagcaggc agctttttac ttcgcaaatt
     4021 aatgacaaaa gaaccgccgc aagtagaccc gccaccgcta tctacaggcc aagacgaaga
     4081 acaaaacgat gagcaaacag aaacaaattc accaggggag ccagctgtcc tagtgcagtc
     4141 cgatactagt acggaagaag tacattttat ggtcagtaat gctgccgaat taacggcgga
     4201 actatcgttt tctagtccgt gttggatacg tgcctttagt gatggtaaaa ggagcctgga
     4261 tggcacttac cgtccagggc agccacaagt agctagggca tctgaagaga ttagtatacg
     4321 tttaggtaat ccgcccgcag taacattggc aattaatggc caagcagtgc agctgccaga
     4381 gacaaaatat gcttatacaa ttactattag taaaaaatag agaccgggaa caggcaactg
     4441 ttcccttttt tattgctata ttttgtccaa aaattgttca gctgacgaca aaatttagtt
     4501 tttgacaact taagggggct cctttatact agacaggaaa gctagaagga ggaacgcagc
     4561 atgtctggcc ttaagattgc cttagtatca ttaggttgtg ccaaaaatct agtagatagc
     4621 gagattatgt tggctcacct acaggaggaa gagttcgatt taattaccaa cccggctgaa
     4681 gccgaggcgg ttattattaa tacttgtggc tttattgaaa gcgccaagca agagtcaatc
     4741 aacaagattt tagagttagc acaattaaaa aagaaaaaat gtcaagtact gctggctgct
     4801 ggttgtttag cgcagcgata cggggaagaa ctgcttattg ctatacccga attggacggc
     4861 atcttaggca ccaatgacct tcaggccgcg gctgcggcta ttcgtcgcac cctggcggga
     4921 gaaagagtaa gctttttaca aggtgaatac gacgcgccgg aggatgcccc acgtattctg
     4981 gcaactccta cccatacagc ctacctaaag attgccgagg gttgtgacaa cctttgcaca
     5041 tattgtgcca tacctgctat tcgtggacct taccgcagtc gcagccgcat agctgtggtt
     5101 gccgaagcga agcagttggc gcaaaacgga gtgcgggaat taaatataat agcgcaagat
     5161 atcacgcctt acggtctaga ttccagtggt gaattagaat tgcctatctt gctggaggaa
     5221 ttagcacagc taaccggcat cgcttggatt cgcttacttt atgcctaccc agagcgtctt
     5281 gatccgagga ctatcgaaac aatggcacgt ttaacccaag tgtgcaatta ccttgatctt
     5341 ccactgcagc atgcctccga tcgcatccta aagcaaatgg gtcgtaaatt tacggcggct
     5401 aagatgatgc agctattaac acaacttcgc caaatgatac ccaatattac cttacgctca
     5461 tcttttattg ttggttttcc tggcgaaaca gaagctgatt tccaagaact tttgcttttt
     5521 ttacgcgttg cccaacttga tcgcattggt ttctttgcct attcgcaaga agaaggtacc
     5581 cctgcggccc gtctgccaca gcaaatttct gcccaagtta aagcagcaag actagctgaa
     5641 gcgatagctg tacaaagtga aattatcacc gctaagcaag aagcactagt gggccaaagc
     5701 tttatggcga ttatcgatgg gcgctcggca caagagcctc agaccttgtt gctgcgcacg
     5761 ccgatgcaag cgccggaggt ggacggctat gtcagggtag cgaactgcga cgctgctccc
     5821 ggaacaatgc tgcctgtaac tatcacggga tatgacggct atgacctctt aggcgcagtt
     5881 gaactgtcac attaactagt aaatatttat gttaacctaa caaacaggga acgatctcgg
     5941 tcgttccctg tttgttagca aaaaatatcg tttgtagtct tacatctctg tgttgcaaaa
     6001 gttcgaacta tttccagttg gcatagattg ttagctatgt tataatacat tatgtaaact
     6061 tcttttactg ctagaaaggg cgatactatg aaaaacttca agtggcaggt ggccgtgtgc
     6121 gcctttgttt taacattggg gttagcagta ggtgctgtca tgctgcgtca acggcaagtg
     6181 cttgacgaac cgttacgaga acgtttaggc agtttagcag cagtagaaac tgttgaacta
     6241 gatcaagaag aagaaaagtt tgtcgtacga gttaagcttg ctgaggttaa ggatttagcc
     6301 ttaacctacc gagccttaga agcagagctt aatcagctgc tagcagagga tagctaccgt
     6361 ttagtactgg tggatgaacg caactcagaa ctcacagagg cgttatttac tgttagtctg
     6421 gctctttatg aaggtgaaca aagaggaaat tttacagaaa tgagtaaagc tattgccgca
     6481 actttagaag aattgcaagt agcagaacat cgtcttacag ttgatcatga gcatatttat
     6541 ctgcaaatga aaaatggcga cgcttacttg tacgaagtgg taggacgtga gccgtggaga
     6601 agggagggtg agcgggtatg atcatggaaa tagcggcggg tgcagcactg gccatttttg
     6661 ttgtactggc ttggcagggt gtaaatattg cacctgctgt ggtgctagcg gtcatgtttg
     6721 cactgttgta ttattttgcc gggatgcgca gcggaggtaa aaaacaattt gctgttgttg
     6781 aaggcagcag gaatacggga ttagttaagt ttgaccaaat cggcggtcaa cgtaccgcta
     6841 aaaaggaatt attagaagca cttgatttta ttcgtcaccc tgaacgtata gccaacatgg
     6901 gcatccgtcc actaaaagga attttgttag ctggtcctcc cggtacgggt aaaactttgc
     6961 tggcaaaagc tgctgcggcc tataccgatt ctgttttttt ggtggcaagc ggttcagaat
     7021 ttattgaact ttatgccggt gtcggtgcac agcgtgtgcg gcagattttt tctaaagctc
     7081 gcagtacagc gctgaaagaa aagaaaaata gcgcgatcat ttttattgat gaaatagaaa
     7141 tccttggtgg taaaagaggt tctcattcct ctcaccatga atacgatcaa acgctaaacc
     7201 aactgttggt ggaaatggat ggtttagcag tcgaagaacg tgtgcgtaca ttgttaattg
     7261 gcgctactaa tcgtgctgat ttattggaca gtgcactaat gcgccccgga cgttttgacc
     7321 gcgtggtaca ggtagatttg cctgatttgg aagcgcgtca ggaaattatt aaaatacacg
     7381 taggtaataa accgctggct gctaatgttg atttagagca gctggcacgg gaatcctttg
     7441 gtttctctgg cgctcattta gaaagtctgg ctaatgaagc tgctattttg gcgatgcgtg
     7501 aacagcagcc tgaaatttca actcgtcatt ttacagatgc cattgataag gtgatgatgg
     7561 gtgagaagct agatcgcaaa ccagatcaag atgaactatg gcgtgttgct gtccatgaaa
     7621 ttggacatgc cctgattagt gaactgcagc gacccggttc tgtgtcgaat attactacta
     7681 cgcctcgggg taaagcactg ggttatatgc ggcaaagtcc gcaaaaagat agttatcttt
     7741 acacacagga gtacctggaa ggacaaattg ctgtgatggt agccggttct gtagcagaag
     7801 aagtagtatt aggaaatcgt tctacaggtg ctgctaatga ctttcagcaa gcagtgaagg
     7861 cggcaaagca gattattgcg gccggtatgt cttcattagg gattgtttcc ctagaggacc
     7921 tgcctcagga tcttttacac caagagatac agcagatcat cgcccaacag aaaacagagg
     7981 tacaggcatt tttagcttca cgcaaaggat tagtctcgga aattgctgag atcctggttc
     8041 atgacgaaaa agtcacaggt gaattattac gcagcaggct ggaagaagac cagatggctg
     8101 tgtagcaaca agattatctg gaaagattaa gtattgtagt ataaaaagca gctagtttca
     8161 aaaatgctct tgtcagcttt accaaagtgc gcgtgcaaaa aactcccgac cttagcggtt
     8221 tgaggagttt tttacttggg ccatgatcaa gcgagagact agatcacagg cacctagatc
     8281 ttctgtatgt aaataatgat tagcagtgct atcaaaaaga atattggctt gtgccggcaa
     8341 gccgtcttct tcatcacccg cccagatttg aaagagcagt ggtactcgtg gtaatagata
     8401 gattaagaca gcattaccgc ttattcccgt tagcggcaca ccaccgaaag gctgtgcggc
     8461 tgtctgtaat agctccggct gttgtccaaa aactttactc agcggttcaa tagctgtttt
     8521 gacaaaagca ccatagtaag tctgaccacc ttcaaggtga cgataaggaa tataattgta
     8581 ggccaggggg ataccatcag cgcgcgccag ataattaatc attattaact gcagggcgaa
     8641 aggtggttgt aaatcgctat ctttgtaggt taccaggcct agggggtagg taatggtgaa
     8701 aggatggttc aggcagcaga cggtaaaata gctgcactga tgattgtaga tacagccgct
     8761 taattctgcc atcttctcgg gctggttagc agcaaatctt tctcttgcca catcaagagc
     8821 aatttgatag ttttgcggat tcaaatcatg ggctaaggga atggctgttt tttgcatctt
     8881 aacaccttcc tgtttgtcag tattagtgat ttttctgttc atctttgtgc aattgtagta
     8941 aactatgttt agagtgacga ttggagtgag cctttaatga gacaagataa attagattgt
     9001 cgaggttttg cctgtcccca gccagttgtg gaaactaaga aaaagttggc tgaactgtct
     9061 gtaggcagcc ttaacgtact agtggataat gaaacagcaa aaataaatat tttgaagtta
     9121 gcaagcacgc taggactcgc tgccgcggca aaagaagaag cagattatta tagcattact
     9181 ctagtcaaag aagagcaagc cgcggcaagg atcgcggaat ctggtaaaaa tattttaatt
     9241 accgccgcga cattaggtca cggtaatgat gatttaggca caatcttaat gaaatccttc
     9301 ttttacgctc tggcggaaag tacaaagctg cctaaagcac tatatttact gaatggcggt
     9361 gtgaaattag cttgttccgg ttcacctgtt ttagatagtt tgactaaact acaagcttta
     9421 ggggtggaaa tattctcttg tggcttatgt ttagattacc tgcaattaac cgcacaactg
     9481 caggtgggtg aaatcaccaa tatgtatgcg attgtggagc aaatcacctc cggtacagta
     9541 attgaactct aaccaagcag gataataggc aaaaaatagc gaatatatgt ttgcataggg
     9601 aaaaaagggg agatagcttt gcgtatttta ggtatcgatc cgggtattgc cattaccggt
     9661 tttggtgtga tagaaacaga tgggcagcgt agttctgccc ttgcttatgg ctgtattcgt
     9721 acagctgcca gcttaagcat gccagagcgt ttagaacaag tatatcgaga actgcaagag
     9781 ctgatagcgc gtcatcaacc acaggaagca gcgatagaaa aattgttttt tgctaaaaat
     9841 gctaatagtg ccatgcaggt gggagaagct cgcggtgtgg ccgtgcttac tgcaagaatt
     9901 gccggacttc aggtctttga atatacaccg ctgcaggtaa aacaagctgt agttggttat
     9961 ggcagggcag aaaagaatca ggtgcaacaa atgatgaaaa tgctgttagg gttagatcag
    10021 cttcctcgcc cagatgatgc ggcggatggg ttagcaatag cgctgtgtca tgctcatagc
    10081 ggtgccggcc gcgccgcttt acaacggagg aaaaactatg tttaattatc ttcgcggtac
    10141 cctgatagat aaagaaaaag agacgatagt cctggagaat agcggtattg gctggcaaat
    10201 tactgtacct ctgactgtgc aggctcagct agcctctgta ggccaagaaa tcaagctata
    10261 cacctattta gcagtacgtg aagatgccct gcagctatat ggcttttcac aaagagaaga
    10321 actgaccttg tttaagctgc ttatttccgt gtctggggtt ggacccaaag cggcactcgc
    10381 tgtattatca acattatctg ctggcgagtt ttattgggcg attatgcatg aaaatatcaa
    10441 agcattgacc cgcgtacccg gtattggacc taaatcagcc cggcgtttga tagtcgagct
    10501 gaaggagaag gctacgaaca tcgccatgtc cagaggcgaa gcgcagcacc cggctgctgg
    10561 acaagcagct gtggcgatgg atgcttacag tgaggcctta gctgccttgc tatctttagg
    10621 ttatcaaggt acagaagcac aaagtgcttt aaatgaagta ccggagcgtg aacagctagc
    10681 gacggaggaa ttattacgta aagcgttagc ctatttaggt acgcactaag gagattacca
    10741 tggaagagag aattatctca gcgcagcgga gcaatgaaga tgacacccca gataatacgt
    10801 tacgcccacg cactctagct gattacattg ggcagcaaaa gttgaaagat aatttacgtg
    10861 tgtttattga agcagcccgc gcgcgcggtg actcactaga ccacgtatta ttatatgggc
    10921 cgccaggctt gggaaaaaca acgctttccc agatcattgc cacagaactt ggtgttaatc
    10981 tgcggtctac ttctggtcct gccattgaaa gacctggtga tttagcggct attttaacca
    11041 atctcgcccc ctatgatgta ttattcattg acgaaattca tcgtcttaat cgtacagtgg
    11101 aggaagtttt atatccggca atggaagact ttgctttaga tattattatt ggcaaaggac
    11161 ccagtgcccg ttccttacgc atagatcttg ccccatttac cttaattggc gcgaccactc
    11221 gtgccggcgc cttatcttca cctttacggg accgttttgg cgtggttgcc cgtttagagt
    11281 tttaccaaga gcaggagctg caaataatag tacgtcgcgc ggcgcaaatt cttcaggtaa
    11341 agattgatga agagggggcg ctgacgattg ctcaaagtgc gcgaggcaca ccacgcgttg
    11401 ccaatcgttt actaaaaaga gtacgtgatt ttgcacaagt gaaagccgat aactttatta
    11461 atgctgaagt ggctaaacag tcactacggc tgctgcaagt cgatgaacta ggattagacg
    11521 aagtagacag acatttactg cggacaatgg tggagaagtt taatggtgga cctgtagggt
    11581 tagatacttt agcagcggcc atcgctgaag agtccgacac tattgaagat gtctatgaac
    11641 cctatttaat gcagattggc ttcttgcagc gcaccccccg cggccgcgtg gttacagccc
    11701 gggcttgtcg gcacttgggt ttaacttata ccgaaaacaa agaacaaaac actttatggt
    11761 aggtgaaaga agtggagcta tttttgcata tgtgttgtgc accttgcagc acttatagta
    11821 tcaaacactt tcgcgcttta ggctacgata ttcatggtta tttttataac cccaacattc
    11881 acccctataa agaatttcag cggcgcctgg aaacactgga agcatattgc caatctgtca
    11941 atgtgccttt aacagtgcaa agaaattatc atttagaatt atttctgcag catgctgtga
    12001 gtaatttgga cgatcgctgc cgagaatgct atacattgcg tttgtttgag gcagctagga
    12061 gagcaaaaag cttaggcttt accaaatttt ccacaaccct ggccattagc ccctatcaag
    12121 atcaccaatt actgcaggaa atagggcaaa cagcggcgca gcagcatggt gtggagttta
    12181 tttatgaaga tttacgtcct ggctatcgcg agagtattaa catctccaaa gcagcaggct
    12241 tataccgtca agcttactgc ggctgcattt tcagcgaaaa ggatcgctac tataaaggtg
    12301 atcaactaga ggataaatag tagtacagtt gaattagatt tagacaagat gcggtataat
    12361 atcgttacaa ggaaaaaaag taaaacgaat tccttttggt ggtggttctt tgtttctagc
    12421 tgactttgat tatgaacttc cggaaaactt gattgcacaa gttccactgg cacagcggga
    12481 agcttcccgc ctgcttgttc tacataaagc taatggcaag tgtgaacacc agcaatttac
    12541 ggacctaaaa cattatttac gcgctggtga tgctttagtt ttaaataata caaaagtact
    12601 gccagcacgc ctgctgggac acaagaggca aggcggcgct ctcgtggaaa tattattgct
    12661 taaacagcgt gatgcacata cctgggaaac tatagttagg ccgggtaaac gtctccgccc
    12721 tggggctgaa gttatctttg gtcagggaga gttaaaggca cagattttgg agatcttgcc
    12781 tgatggtggc cggcttgtta gctttacttt tgctggcacc tttcaagaaa ttcttgatcg
    12841 actgggggaa atgccgctgc cgccttacat caaggaaaag ttgactgata aagagcgcta
    12901 ccaaactgtt tatgctcagg aggaaggatc ggctgcagcc cctacagcag gcttacattt
    12961 tacgcctgac ttgctccagc aaattgcaga aagtggcgta tcactagttt acttaacttt
    13021 acatgtggga cttggtacat ttcgcccagt taaaaccgag cgtattgaag aacaccaaat
    13081 gcatagtgag ttttatcagc ttccggcaga agctgcagat acccttaatc aatgtaagca
    13141 gcgcggcgga cggatttttg ccgtaggtac caccacttgt cgtactttag aaacggtaac
    13201 atcggcagac ggtattattt atcccggtag cggctggaca gatattttta tttatcctgg
    13261 ttatcgtttt agggctatcg atgccctgtt aaccaatttt catttaccta aatctacact
    13321 attaatgtta attgctgcct ttgcgggacg tgaaaatgtc ttacaggcat atactcaggc
    13381 agtacaggct gaatatcgct tttttagttt tggcgatgcc atgctgattc tgtgattgac
    13441 agcgggtata aagacagtta aactaacctt tggagagatg taaatggctg taaactatac
    13501 tttgatcaaa gaatgcccca gcaccggtgc acgtgccgga atcttacata cgccacatgg
    13561 tgatattcct acaccggtat ttatgcctgt aggtacccag gcgacggtaa aagcaatgag
    13621 tccggaagaa ttgcaagaaa ttggtgcacg cattattcta agcaatacat atcatcttta
    13681 tttgcgtcct ggccatgagt tggtgcgtga agcaggcggt ctgcatcgtt tcatgaattg
    13741 ggatcgcgct attttaacag atagtggtgg atttcaggtt tttagtttag gcgctttacg
    13801 caaaatcaag gaagaagggg tgacttttcg ctctcatctt gacggctcgg aacatatttt
    13861 cagtccggaa aaagctgttg agattcaaga agccttaggt tctgacattg ccatggtttt
    13921 tgatgaatgt gctccatatc ccgccgaacg tgattatatc gaggattcat tagcacgcac
    13981 gacacgctgg gctgaacgtt gccttaaagc gcatactaga gaagaacagg cgctttttgg
    14041 tattgtgcag ggagggatgt atgctgattt acgccaagag agtgctcgtc agctcgtagc
    14101 cctagatttc cccggctatg gtattggtgg cttaagcgtg ggtgagccag cggcattgat
    14161 gtatgaaatg ctggacgaga ctgtgccaat cttacccgca aataaaccac ggtatttgat
    14221 gggagtaggt actgccgatt atttaatcga gggtgttaaa cggggcgtag atatgttcga
    14281 ttgtgtactg cccacgcgca ttgccagaaa tggcaccatt atgacgcgag acggctacct
    14341 aactgtgcgt aatgctccct atgccaagga ttatgggcca ttggaagaag gctgtaattg
    14401 ttatgcctgt cgtaattatt cccgagccta tatacgacat ctcataaaaa caaatgaaat
    14461 tttgggtttg cgtcttacct cgtaccataa tctttatgtc ttagtacact ttatggctcg
    14521 tattcgggat agtatactta atggtacatt cccggacgtg tataaagaat ttatcgcccg
    14581 ttttgggcaa gaaaaggagg agaagtaatg gacactttaa aactacttgc cccaatgcta
    14641 atcttttttg cgctgatgta ttttttaact attcggccgc agcagcagca gcaaaaacat
    14701 cgaggtagta tgttatcaaa tttgtctgta ggcaacaaaa tacgcaccat tggtggtatt
    14761 tttggcacca ttgaaaaaat caatgatgac gaattaaccg ttcgtgttgc agatagtgta
    14821 agcctacgga tggctcgttt tgctgtagaa tctattatta aagagtaaac tcggcataaa
    14881 cggcataatt tgcgcattga gtacatagat tgcaagtaag aacttgtgga ggtgaactca
    14941 atgccaagga aaaataccgc tgccatgcaa atccgcccac caacagctat tttggtgatg
    15001 ggcaaaggag tcctctacgc ctactttttt tcactagtgg tttttcttgt ctttagtatg
    15061 cttatccagt atacaagtct gacagaagct atcttgccct acacggcgta tgcaacttct
    15121 ttagtagcta tctttattgg tgccgcttat gtcaccagga ggctgcagca aaaaggctgg
    15181 cttaacggag gtattaccgg gctgctgtat ttagttggtc taataattat tgctatggtt
    15241 gttttaccag attttagtct aaatgccagc tatttttcta aagtagtact tgcttttgct
    15301 acaggtgcgg ctggtggcat ctttgggatt aatacgtaaa gagaccgttt acttaaaacg
    15361 gtctctttac gttatgatct ggatagatga gaattctttg gctgtaaaaa gtgattaagc
    15421 ccaagatagc agtgggtaaa acctgatcta agtattagta cactcacccc aagtctggca
    15481 aaactaaaca gcagagggga cccgtgcagt gctaaaagct gagcaccaag catcaacaat
    15541 atgaaaataa gtggagaaaa ccgtctccaa cctacttgtt gctgccgtct ggcagcaacc
    15601 atagatattg ttgtaattac taagataaca ctagttattg taacctcaat cccactggtg
    15661 tttaatctgc cgaaattttt tagtgaagca agaaccagta aaaaaccaag tatagaataa
    15721 actagataac caacaaggag gtaagccagg taccattgcg atctaggttg tgttttggag
    15781 ctgccagcag catattctct tcctgtatag ctcaataagt ataatcctag cgacaacaga
    15841 gtagacccat tacgtaaata aacagcagta agcagacaga caagcaaaag caggctaaag
    15901 agcacttttt gcttacaggt gtttactagc atcattacca cctccgtcaa atcttctttg
    15961 cttagcctat gttcataagc taacttttgt gattatcaga agttgtgctt ctgttgatgg
    16021 cttgttttcg agaatagtgt ttgtagttga cagcatggca aatcatatta tatgataatt
    16081 atggtgatat ttgcctatta gtagctgctt gcagataata ttctgaaatt tcacagctgt
    16141 ccaaattata agctgcgacg cagaaaaatg ccggtagtga aagtaggaaa aaagtggggt
    16201 gtaaaaaaag acaggctatc ttcgttgcgg ctattatctt taactacttt ttgagaaaag
    16261 gagtggtaaa aaatgacgag cgatattaat aagctgtata atgagcgtct tggccgttat
    16321 caggccgcca ttgctctgga gcctacagac cgtattccca cagcttttgc aacgacctac
    16381 tttgctgaaa aacaatctgg ctattcgtac caacaaatca tgtatgattt tcaaatatgg
    16441 gaaaacatgg aagtagggtt tgccaaaaaa tatcctgatg tggatatttt ccggaccaat
    16501 ttaggttggg cagcgacgtg ggatattacc aaccatcaac tgtggcgagt gcccggacgg
    16561 gatatttctc ctaactcttt acagcagttc aatgagcaag aatggatgaa agctgatgag
    16621 taccgtcgct ttattgacga ccccattggc taccgtatgg atgtttattt gccacgtatc
    16681 ctcggtgaat atcaagagcg cggctcaatt cgctcttata tggcttttct taaagccggg
    16741 atggcaggta gtatttttgg tgctgccgca gccaaaagag ttgagcgact gaaaaacgaa
    16801 gttggcatac ctcaaggtac agtagggggc ttttcagctc cttttgatgt cttatcagat
    16861 gcctacaggg gcttttacgg tataacgcgc gatatgttcc ggcagcctga caaagtgtta
    16921 gaagcctgtg aacatatttt agaacagcag ttaagtaatg cctggcgcag agctgatcct
    16981 ttaaaacgtt tccctatttt tattgctacc cataagccgt gtttcctctc gcctaaacaa
    17041 tttgatactt tctattggcc aaccttctat aagggcgtca tgaagttaat tgaagccggc
    17101 tggaaattcc gtattttctt agaaggcgat tggggcccgc actggcacca tatggcagaa
    17161 tttcctaagg gtactgtcat ctgtgatatt gataacgaag cagatatctt tgatgccaaa
    17221 gaaaaatttg gtcatcaaca gtgtattact ggtggtatac ctaccgatat gctgattctt
    17281 ggtaaaccag aagatatacg tgctcgggtc aaactcttat gtgaaaccat tggtaaagat
    17341 ggtggttgga taccaaatgg tggtgggcat attccagaag acacaaaacc agaaaatttc
    17401 caagaactgt tggatgcagt aatggagtat ggtaaatatt cagatggacc ggctccagaa
    17461 cccaagattg gcgaacccgg cactaatggg gtcgtattcc ctgaaccagg catagtaaca
    17521 ccttgggaag aaattaaaaa ggaaaataaa tggaaaatac caggtgatga aaaaataatt
    17581 aaggattggt gggaacagct cgaaagtatg gcctatggct ggatttgcag ccaatagaaa
    17641 ggaggacgtc ataaatgaca aaacttgctg tagcactacg tgacctaaat gaggatttag
    17701 tatatcaact tgtagctgaa aagctatccc aaggtatctc ccccactgaa attattaagg
    17761 aatgcaacgt tggcattgcg gctgttggcg atatgtttgc ctccggtgaa tattatttaa
    17821 ctgaattgat gttttctgct gaaataatgc agggcgtgat ggccaagctt gagcctttaa
    17881 ttgcctctag tggtgaagct gaacagactc ttggtacgtt tgttatcggt actgtcagtg
    17941 gtgacattca cgatattggt aaaaatattg tggtaaacct aatgcgcagc catggcttta
    18001 aggtgattga tttaggagtc gatgtaccag cagaaagatt tgtggagatg gtacgtgaaa
    18061 gcggcacaaa aatactaggg ttaagtgctt tgttgaattc cacttacccg gaaatgaaaa
    18121 gtgttattga ggcgctcaaa gaggcgggct taaaagaaca agtcaaagtt attatcggcg
    18181 gaaccataac cagcgaagcc gtacgtgaat ttaccggtgc tgatgctttc gccactgacg
    18241 cccaaaaagg tgttagtttt gccaaaagtg tctacggaat caataactaa gatacttttc
    18301 gaagatttaa ggatcaccca cgttaaaacg aacacagggt gatccttttt atgctccata
    18361 ggaagtattc agcagctagc gcagcggcat cggtcgttca gcttgagtct gtacggaaag
    18421 aaaaagacag gagtgcgctt ttcaatcaca attattctgt aacatagatt ggaaagagtt
    18481 taataataag tgaaaaagac cgtatctacg acatttttcg tatagacaac ctagtaatca
    18541 atacgatata atat
//
LOCUS       DASRMS010000186        39019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_922330, whole genome shotgun sequence.
ACCESSION   DASRMS010000186 DASRMS010000000
VERSION     DASRMS010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 39019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39019
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922330"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     regulatory      243..421
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(581..2143)
                     /locus_tag="VFC74_08725"
                     /pseudo
     CDS             complement(581..2143)
                     /locus_tag="VFC74_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896857.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(2311..3858)
                     /locus_tag="VFC74_08730"
     CDS             complement(2311..3858)
                     /locus_tag="VFC74_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003516184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HZK25459.1"
                     /translation="MKVQDSIKLLQEQKALIALGGGEKRIAAQHEKGKQTARERLQAL
                     LDAGSFTELDVFVQSGLEDAGKVGHSNPGEGVVTGFGTINGRPVYIYAQDFTVAGGSL
                     GEMHAAKICKVMDLAMKTGVPCIGLNDSGGARIQEGVQALNGFGEIFYRNTLASGVIP
                     QISVIMGPCAGGAVYSPAITDFIFMVEQTSQMFITGPQVIKAVTGEDVTSEALGGAKR
                     HNETSGVAHFAASNEADCFIKLRQLLSYLPANNLEDPPRVASSEPLQAAEEMLTIIPD
                     NANKPYDVREIITHVVDGSEFFEVHQSFAKNAVVGFARLGGRSVGIIANQAKVMAGVL
                     DIDSSDKIARFVRFCDCFNLPLITFVDVPGYLPGVQQEHNGVIRHGAKVLYAYSEATV
                     PKISVILRKAYGGSYIAMSSRSVRGDLALAWPAAEIAVMGPEGAANVVYRKEIAAADD
                     PAAERASRIKEYRGKYSNPYITAARGWVDDVIDPRATRSYLLNGLTMLATKQEDRPRK
                     KHGNIPL"
     gene            complement(3960..5645)
                     /locus_tag="VFC74_08735"
     CDS             complement(3960..5645)
                     /locus_tag="VFC74_08735"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HZK25460.1"
                     /translation="MPIIKVVLIGADNADTSLYRVLREIEHVQIMGVMGQNLNALGMI
                     MAQRDQLDVTAELAALVTKPDLDVIIDLTGDVDILQQIKQLKAAKTQVLAAPADSLLM
                     AIIKSKIDEKKLKSELWVILNSMQEGVAIADSNGVLKYFNPSFAQMTGVNSKQKVGSN
                     IFEVNPHGALAQTLMLNRALTNFRTKIENVDVLANTAPIMVDGELDGAVAVCQPITNL
                     LKLMKDLRKSTTIIENLYDRIHQITNAKFTFEDLKGKSKLFSSTVDSAKRAVRSNLPI
                     LLVGEGGTGKEVFAQAIHRASLRHHNSYLKVSTTAIAKELLESELFGHERGAFAGAGK
                     TVLGKVEMVKGGTIYLEDIAGLSLDLQQKLVRLLLEGEFQRLGSEEVQHADVRIIAST
                     KRDLKELAAKGFFSAELYALLKVFTIKLPPLRQRREDIPVLAKHFINVYNRKFDKHVT
                     VIEPEALQLLVDYDWPGNVLQLENIIERAMFLVENKRLKCQHLSPYISQFYEERPPQV
                     TEIIPLAEMEQIMLKAALSRFGNSLEGKKKAAKALNISLATLYNKLKKYQSNR"
     gene            5815..6654
                     /locus_tag="VFC74_08740"
     CDS             5815..6654
                     /locus_tag="VFC74_08740"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK25461.1"
                     /translation="MQVYALIGASGTGKSHRASFLAFKYEIPLIIDDGLLIAGTSVLA
                     GKSAKRETTRYGAVKRAIFYDDKHAQEVQEKIKASGAESILVLGTSDKMVLQITKRLD
                     LPAPDKIIRIEEIASQRAISKALELRQKENRHVIPIPTFAIKKEFPGYLIDPLRSFWG
                     KNRNDSKKVVIERSIVRPIYNSFGSNYISEQVIMQMVTHIAAKLNGVSRVGKVEVLSK
                     PEGTVNLQVEVNVFYGHFIPEVLQHIQSVLKEEMEYLTGLTINNIQVTARRIDLGTNF
                     QLQ"
     gene            complement(6731..7750)
                     /gene="ltaE"
                     /locus_tag="VFC74_08745"
     CDS             complement(6731..7750)
                     /gene="ltaE"
                     /locus_tag="VFC74_08745"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HZK25462.1"
                     /translation="MKIIDMRSDTVTKPTQAMRQAMAEAIVGDDVYGEDPTINRLEEM
                     AAAVMSKEAALFVPSGSMGNQLAIMTHCERGVEAICDSLAHVFHYEMAAAATLSGVQL
                     HPLDDLHNEAGIANLSEHIRNPQDFFPRTRLVCLENTLNRGGGTVMKPEQMAEVYRLA
                     KEYGLNVHLDGARIFNAALALGCEPADITAHADSVMFCLSKGLGAPVGSILAGSRQFI
                     DQARRNRKMLGGGMRQAGILAAAGIFALEDTSFLMADHEKAQYLAQALAVVPGLKVDL
                     TKVQTNMVMVELEQMLVHDFLQKMQQQGVLAGTSGSAAVRFVTHRDVSLTEVQAAVEI
                     IKALC"
     gene            complement(7776..8558)
                     /gene="fdhD"
                     /locus_tag="VFC74_08750"
     CDS             complement(7776..8558)
                     /gene="fdhD"
                     /locus_tag="VFC74_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012158445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HZK25463.1"
                     /translation="MDSRMEMRPILRINGERQESFDDPVVREVPLTIYLNGQEFVTLL
                     YTPELPEMLAVGFLRSEGLIRDYRDIISLRYDEQQSFIFVETEGSKLTEKLYGKRTIT
                     SGCGKGTVFYSVLDALKSSPVQSDFSLSVFQVLQLMKQLQVRAELFKLTGGLHSAALC
                     SAEEILYFCEDIGRHNAVDKLIGLCLKQKISLADKVLVSSGRISSEILVKTAKLGIPV
                     LVSRAAPTTLSIELAENLGITLIGFARGRHCNIYSHQRRVLI"
     gene            8906..9469
                     /locus_tag="VFC74_08755"
     CDS             8906..9469
                     /locus_tag="VFC74_08755"
                     /inference="COORDINATES: protein motif:HMM:NF026229.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4489 domain-containing protein"
                     /protein_id="HZK25464.1"
                     /translation="MMSVSKANHTRYPSKICHGSSCHKPHKTSDIILECGCSPEAAFF
                     EIDDGRVEEGQKFVLDRVRIDITHLIKPVIKLDFSSLIIFEAEDEEGSEHEVEVDLLF
                     KLIRISNGEKEVIRSWSYLYEIDVENNIDELEVEMKQPFAVTFCDKPCATVCEYIMKV
                     EGIDFEGDFDELRVVSPTLSAIAQGQC"
     gene            complement(9632..11203)
                     /locus_tag="VFC74_08760"
     CDS             complement(9632..11203)
                     /locus_tag="VFC74_08760"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HZK25465.1"
                     /translation="MRLKLFLFLITLAFFMVLSIFLLLLITGTLNTGVGEVQKDVEKE
                     LAYISQDISDQYVRYSAHAIELSKVLSASIEMKLKMRGLSTSDLKNHPELLEEIVGGE
                     YAASLSALQRAKSSGVFMILDATVNPALEKAENSKAGLYIKNMEPNILSSTAAYLFVL
                     RGFPSIGRDNAITLHSQWRMEFDVTNAPYYRRPIKEATDHELALSRLYYWSDVITLPG
                     ANEEIMLCSVPLINSKGHIFGVCGFEVSAMLFKLSHTPNIDTYQRVFSVLSPTSEDTL
                     NISQAFSAGSYSPRNRAKTDQTLTIKEGHPFMSYRLDDGTLYSGLHRQLNLYPEGSAF
                     AAEKWAVAVMLPDEDISAAFIKANLRLTLLLILLLAIGSIISYLLSKRYLKPFAESME
                     MIKSNDFSEAAKTKVVEIDDLVEFLSSHQEVTQPLLTQGENINLPTSAYEMFAKNTEL
                     LSPAERAVFNLYVEGHTAKEITGILNLSINTIKTHNKRIYMKLNVASRDELLVYVNML
                     KEAGSEILQQEKDTE"
     gene            complement(11328..12713)
                     /locus_tag="VFC74_08765"
     CDS             complement(11328..12713)
                     /locus_tag="VFC74_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HZK25466.1"
                     /translation="MKIRKQIAVLIVSMMIFAMLSGCQNNHGLDANKPVPLTLWHNYG
                     GQMKDTMDKMVDEFNATVGAEKGIILSVTSISSSSALHEKLIMAANREPGTPEMPDLT
                     TANPKTAVILAEKGLLTNLAEQFTEEELTAYVPRFLEEGRLEGENLYLFPTAKSTEVL
                     FLNKTIFNRFSEATGASHEDLQTFEGLAKTAKLYYEWTDAQTPGTLNDGKAFFHMDSL
                     FNYTQVGGKQLGYDFIVRGQLDDSSEAYQRVWNLYYEAAVRGYFAIYDGYASDLAKTG
                     DITCSIGSTAGVLFFDHLITYADNTTEPVELMILPYPIFEGGRKIAIQRGSGMLVTKS
                     TKTKEYAAGVFLKWFTSPQNNLRFVATTGYLPVTESAFGEIMAEEIAVIEDKNIQTLL
                     TSVVEMQSQYDFYIPPIYEGVDVMQKDYETKLKQAATESRKTYQTLLAKMSRDAAFNE
                     ASQGVFEVFVR"
     gene            12968..13348
                     /locus_tag="VFC74_08770"
     CDS             12968..13348
                     /locus_tag="VFC74_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014187614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25467.1"
                     /translation="MTDDSGKNGRPEQPFAPADIEQHKTMAGLAYAICFLPLIACKDS
                     PYGRFHANQGLLLLLLIMVGSIVLSVVSSILIALTWRLLWLSSLLYGIFSLLIIYLLL
                     LGLINGLNGKPKRLPLIGGIQIIK"
     gene            13366..14049
                     /locus_tag="VFC74_08775"
     CDS             13366..14049
                     /locus_tag="VFC74_08775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25468.1"
                     /translation="MKKLISYFLILLLISSFVIGCENTSQTEDKTPNSNTQNSNDGTS
                     AEKPPANSDGILGANLSTAYAEMMRNDKYLMRYTMTSEYDGQALEFEITVGVAGDNSA
                     VTNIGEGMNTSVISKNDSTYMIDHLQKTVLEIPADSMMSGEMDDNQIGTEELTYESSG
                     QEDGLAYEKYTTADSILIYYFEGKKLVKIVIEVDEEPVVMNILEMSNTVPDSIFEIPA
                     DYAKTTLSD"
     gene            14128..14595
                     /locus_tag="VFC74_08780"
     CDS             14128..14595
                     /locus_tag="VFC74_08780"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HZK25469.1"
                     /translation="MTDNIFGDLVKSLGSFMPNDDPDIKLFQAQTEISSLEHREQELF
                     GEIGRKEFPHICEQPEYSEIVQELATLQKKLQTARSTLQKVQSEKAEQDRLEKEKLLS
                     RTCSHCDTVNPEGVQFCQECGAKLGSSNKPVCPQCSTEYQAGTRFCGHCGSKL"
     gene            15016..15684
                     /locus_tag="VFC74_08785"
     CDS             15016..15684
                     /locus_tag="VFC74_08785"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HZK25470.1"
                     /translation="MLTAFTRNYEDNSTEAGFQFTFYCDLCHDGYKSSFIESETYKKG
                     SLLRGISGGARALGGLFGGKLSDLAWSAGYGGDILSEKFNGMSPEWQKEHEKAFERAQ
                     NEAQFHFHRCHNCRLWICDADFNEDEGLCVECAPRMNIEISTARAKKMVQDIEEKAQA
                     SSVFDGKIEAKTIICPACGKPSGTGKFCNNCGTNLSLLKCPSCGSNLAQGVKFCGECG
                     HKMS"
     gene            complement(15809..22156)
                     /locus_tag="VFC74_08790"
     CDS             complement(15809..22156)
                     /locus_tag="VFC74_08790"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HZK25471.1"
                     /translation="MFKKIIAYLLVIALVVVPLAGCGGNQDEVAGEKVPLEQISAVAA
                     PELTGLNKMPEDGIGKPGVDFVEIETGSADDWGDLAIPYFNPLGSYSLYSIVEQVENS
                     EELLLHTLEGLTPKTQAVRGEVWNMSIPLESEISILWLRWYANKLGATRYGSTAEHSV
                     FSVKENDHTTWWVDAQISEEAADLHVVKISTLPLGKEMTISREQINKAEEGEYYFYTE
                     CTPGQLQSATISLKGDANSGVEFRVEQAVNYGSYQYNVSKVISSDELNTKKGNKFVFD
                     DLPLAEGMLCYKFTASSEQLPEEITVCIDNVAEIEKVKWGEASGAIRVTGIPLGMAGV
                     QQPNWATITHSALGENNAFSVTEQNGTMLFNVPAGYYNVSFPSGLGDELSFLRLVPVS
                     AGEVTTVTIPQEMEYAVGAMSRKYGDFDLNEGGIEILTNKDNGATASVSLVLNDPLDR
                     DISLNKEDVAITENGVDAVITNITREPAGSDVVLVLDSSGSMGENMQPCIEAAKRFVS
                     GLPENSNIRLIQFAQKITEHQGAGKAAVLKSLATIKAGGATSLYDATSRAMGLLKGKK
                     RGYAVVFSDGADSREPGVDGTGSALTKDKLLGQIKASGVTILTIGFGKGHDPATLVQM
                     AKSSANGAYFAAGDKNALDGVFASVAGKLGNQFTIDYQRPTVTADESSAVPVVSLMID
                     RSGSMDDDPAEDPDSDVGWRLDGVKAIFHDFILGLPKGTLMQVGSFAEPLGGAEPCYD
                     QITTDKQAPALQAIGSLVAGGGTPTITALQLAQQNLSTVPSSKRVLVFFTDAAISGGD
                     EIEKQLIDEVLAELKNSGIRVLIAGLLNTTNAAAYEAAFQAAAQVAGGDYIVTNNVED
                     IAAKLADLLKRIDEPTPKDGIDFTIQIDCSTADGSRLNLHSGINLENFSPLKSKLGET
                     LKPGMVTMATGEKLVNYDRQAAQLLYGSDLPNVDSRVLFRVPLKDKTAANNFGSLTVS
                     EAYVLDLFKGITPPSGKCFLALNTELTFKAANSADPEKGYRIPNIFNHFYVSYNEGQM
                     MPASEATWLAETPFALPGDPEVTVMKGEKRSGMLVFLADMGTGENVGELSLHMYDTVN
                     GHIQLSLTGGRARNLVNLDSLPTTAANKISEAFSLAITGKKDLTTLEGVSIAQAESDD
                     TSANKQKDVSFRVLEARFDSHVQALLDIDPQKRLLHQLETDQGVLLTEMSNIVYNLPL
                     GFTGKTMLAPGSCNNVRLPFFVPQELLKVKSSIFGDLQEGSLTIPVTEGSPYVTGSIG
                     KTFAHEYFNLKINSLSRLEPDSNHLVLDFTIEDKKDGAGTGGLAGVLQLQRSSEITKP
                     LPGEVRELNAEELKRIAGARKGLGNFADDSEENEGIVAVNVEQTAMLLYGAYNAVGNW
                     GAFDGQKRRGLLVFTLPYESAPADWTLTSTALRDLEVPVSESVYPYQALLAKTPEIPI
                     NSEFENILNEAVTTAVTKYHSTRQEPNLTTRVGLSDAEILGEQVPAPSLTLYGSQLLQ
                     SVRTDEDFLSVMNGQKCVPSTDIAHYYYAPEAVLTQGWGSQYDLAILAHTMLSRLGYR
                     PQHSQVKLTTVGKENLQRLGAVEEIPTWLPAVSYTDAKGELRIYVPVFSRDISELYGL
                     CEISSDVDLGEISPAKSKIIIEMTGKLVGSAGMAATQGMFGDFAAALGGGENGSDFYE
                     TVTVFEREVSLPDMSLDPIDISYIATAKSEGGELILPVLDTKQGLLFDEAGWVDSSNY
                     EFESISIKLGTDINDQVVHTTILGKDQKITEVCHTLAWGIPEMSSEAAQPYEKMVEAE
                     AKAAKDPTNYSIGRWLGHATISRFVKNFTDFGRETAKKLEVTAGRITAPIALIVTMKS
                     DTKKAEAVVDLMNHRNQLHRGEEEKQWAYNLMCGYFASEAEASALPNSEGISYLDVWN
                     GLPKDASMYIMAPTEAGQYEASAQELSENGYPPLLVKRLTAKIEDGAMEGTLYIIPDY
                     PVAIDGKPRWAWLEINQTTYDVVSVFESGERAGMADYLIGLFPENIMEVGVGVITGVT
                     TAMWGVSTFALVLDDYKEIRANAAKLCGIIGERMKTISDATGTIGHINRAGQAFGELY
                     IPGKDIGDALSKLLQSCEAQEIQPSFGLGYKKAVEAYFGVD"
     gene            complement(22555..25233)
                     /gene="fdhF"
                     /locus_tag="VFC74_08795"
     CDS             complement(22555..25233)
                     /gene="fdhF"
                     /locus_tag="VFC74_08795"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015617970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(24181..24183),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HZK25472.1"
                     /translation="MSKVNITINGQALQVDSGMTVLEAAQTVGIDIPTFCHDPELTKV
                     GACRMCVVEVTGARNLPASCTTPVAEGMVVETETTRVKKARKNILELIWADHPQDCLT
                     CEKTGSCKLQDYSYRYDVEGSPYQGEVREIPLDDTNPFFFRDLNKCILCGKCVRKCHE
                     INGVGAIDFMYRGFGTKVTPAYDDPIEESSCVFCGMCVDICPVGALTPKQGLNQGRPW
                     EVDKVLTTCPYCGSGCTFYMHVRNDKIISITPNFEGPMKGHLCVKGRFGWDFVDSPDR
                     LKTPLIKKDGEFVEASWDEALELVATKFKEVGAQYGADAIAGLSSARTTNEENYLFQK
                     LLRSLGTNNVDHCARLUHAPTVAGLATAFGSGAMTNTIGEIENANAIFVIGSNTTANH
                     PVIGYRIKQAARNGAKLIVADPRYIELCDYADVHLQLKPGTNIALLNAMAHVILKEGL
                     ADEKFIAERTENFAALRVTVERYTPEYASEITGVAAKDIVKAARLYGAADAAMIFYTM
                     GITQHVTGTDNVLAVANLALVTGNMGKPASGVNPLRGQNNVQGACDMGALPNVFTGYQ
                     PVTNDSLRAKFAKAWGRELSGKAGLTVSEMFDAAGAKEVRAMYIMGENPYLSDPDSSH
                     VIHCMETLDFLVVQDIFMTETAKYADVVLPAVSWAEKEGTFSNTERRVQKVNPALKPR
                     GNAKADWQITVELANKLGLAWDYTCAEDIFQEIAALTPSYAGISYDRLGVSGIHWPCP
                     TAEHPGTPILHIGKFSRGLGHFSGVEHQGPDEVPDQEYPFMLTTGRILYHYHTGTMSR
                     RSKGLKFMQDEELMQINPADAQALGVTECDTVKVTSRRGSVNSRLNITDTVPKGVVFM
                     TFHFKETAANLLTNPETCPTAGIPELKACAVKIVKA"
     gene            complement(25250..27025)
                     /gene="nuoF"
                     /locus_tag="VFC74_08800"
     CDS             complement(25250..27025)
                     /gene="nuoF"
                     /locus_tag="VFC74_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HZK25473.1"
                     /translation="MEAQSYRVRVGLATCGIAAGASKTYDKLVSLLAEHDIVPERTGC
                     IGMCFSEPLVEVTTPAGETYLYGDATADKVAAIVEQHIVGGKPVEEMLVLSSAAATDN
                     DHFMAKQHRVLLGHVGVIDPESIDSYLAIDGYIALTKVINEMTQEEVIKEVQKSGLRG
                     RGGAGFPTGMKWNFTYQAKGNKKYVVCNADEGDPGAFMDRSVLEGDPHALIEGMTIAA
                     FAIGADEGYIYVRAEYPLAIKRLQIAIKEAKAHGYLGNKILGTDFCFDLKIKEGAGAF
                     VCGEETALLASIEGLRGIPRPRPPFPANSGLWGKPTLLNNVETYANVPVIFRLGADEY
                     GKVGAERSKGTKVFALTGKIKKTGLAEVPMGITMREIIYDVGGGIQDDRAFKAVQIGG
                     PSGGCLPEEQLDLSVDYDSLTAAGAMMGSGGLVVMDETTCMVDLAKFFLTFTQSESCG
                     KCTPCREGTKRMLEILERITAGQGKPGDIETLEEMGAAIRDSALCGLGQTAPNPVLST
                     LRYFRHEYEEHINEGKCRAGVCSNLISYNIIEDKCVGCTVCAKNCPVNAISGERKQPH
                     EIDQSVCIKCGVCMEKCKFDAIILK"
     gene            complement(27065..27550)
                     /gene="nuoE"
                     /locus_tag="VFC74_08805"
     CDS             complement(27065..27550)
                     /gene="nuoE"
                     /locus_tag="VFC74_08805"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HZK25474.1"
                     /translation="MKEAHECHAEDSFDYAPVDEILERYQGQKGTTIPILQAIQEFYG
                     YLPRQAMAYTAKQLKVPFSRVYGVATFYAQFHLTPRGENIIRVCQGTACHVRGASKIV
                     EALKEQLGLDVGETSNDQKFTLESVACIGACGLAPVMMINNDTFGRLTAEKIKEILNN
                     Y"
     gene            complement(27787..28362)
                     /locus_tag="VFC74_08810"
     CDS             complement(27787..28362)
                     /locus_tag="VFC74_08810"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HZK25475.1"
                     /translation="MNEKKELRKKILAERDLLTWDQVKANSLKIAENLFALPQYQAAQ
                     TIMYFLNFGKEVLTMKMVPQTLAHGKRAVATKTVTKERRMILSEIHDLEADLEPGLWG
                     IPEPKPEALRPLEPKEIDFVVVPGVAFDLQGNRLGYGGGYYDRFFPQLRDGVPLVAVT
                     FEVQITNGIPVDPWDRRVDFIITEKRVIDCR"
     gene            complement(28401..29855)
                     /gene="proS"
                     /locus_tag="VFC74_08815"
     CDS             complement(28401..29855)
                     /gene="proS"
                     /locus_tag="VFC74_08815"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009895182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HZK25476.1"
                     /translation="MAKQDKEFVKEITPQNQDYSQWYLDVILKAQMMDYAPVKGCMVI
                     RPYGYNLWENMQEGLDGRIKATGHRNAYFPIFIPESLLQKEAEHVEGFAPEVAWVTHG
                     GNEKLTERLLVRPTSEAIICEMYSRWIQSYRDLPVLINQWCNVVRWEKVTRPFLRTTE
                     FLWQEGHTAHRTEAEAQAETLQMLEVYRDFVENDLAVPVVTGQKSEKEKFAGALRTYS
                     IEALMSDGKALQAGTSHNLGTHFAKVFDITFLDSDGELKHAWQTSWGVSTRLIGAIIM
                     VHGDDRGLIMPPKVAPTQVILVPIMPKKARLEVLEQATAIFTELKQAGLRMEMDDREE
                     YSPGWKFNEWEMKGVPLRLEVGPRDLAAGQAVLVRRDTGVKETLALADLTVRVPALLA
                     EIQANMLEKAKKFRAENTHRTDDYAEFKQIIEEKRGFIISSWCGHSDCEAAVKEETKA
                     TIRNIPFYLQNETPGRCLHCGGEGKHLVIFARAY"
     gene            complement(29992..30915)
                     /gene="cysK"
                     /locus_tag="VFC74_08820"
     CDS             complement(29992..30915)
                     /gene="cysK"
                     /locus_tag="VFC74_08820"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007779734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HZK25477.1"
                     /translation="MQIAQSVVELIGQTPLVRLTNLVEPTMAEVTVKLESFNPSGSIK
                     DRAALGMLLAAENAGKLKPGDTILEPTSGNTGIGLAMLAAARGYRLILVMPDTMSMER
                     RNLLLAYGADFILTPGAQGMQGSLAEVDKILREHPEYFVPQQFANPANPEIHRTTTAL
                     EILQQTEGQVDAFVAGVGTGGTVTGVGEVLKQKLPAVKVYAVEPAASAVLSGEKPGPH
                     KIQGIGAGFVPSVLNSAIIDQVLTVSDQDAMETARQLAAREGLLVGISSGAAVFAALQ
                     LARKLGPGKRVVAIAPDTGERYLSTELFRHE"
     gene            complement(30947..31195)
                     /locus_tag="VFC74_08825"
     CDS             complement(30947..31195)
                     /locus_tag="VFC74_08825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25478.1"
                     /translation="MKYEGIDNWVSIFEAATWEEATMAKGMLEAAKIPVILDRDSLGD
                     GYSMSASVMNEVVVKVPKQMVTKAAQLLQTKSFGASGD"
     gene            complement(31291..32676)
                     /locus_tag="VFC74_08830"
     CDS             complement(31291..32676)
                     /locus_tag="VFC74_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HZK25479.1"
                     /translation="MEKMYTISDLPQLTRTQVRENFKNHMNPGLCSYLGLLDFDKHYV
                     KAQGVNVWDGDGNCYLDFLGGYGSVNLGHNHPYVIESLQKVSAMPNLLQASMNGVASA
                     LAHNLAQITPGKLSNTFFCNSGAEAVEGALKLARIATGRSRIISCVASFHGKTMGALS
                     LTGREKYHKGFEPMLPDCNMVPFGELESLEKELKTNDVAAFIVEPIQGEGGVIVPPAG
                     YLNAAKELCHQYGTLFIADEIQTGLGRTGKMFACDHEGVEPDVMCLAKSLGGGIMPIG
                     AFITTAEIWHKAYGGVEKCLLHTSTFGGNTWATAAGIAALEVINQEKLPERAAEMGAY
                     LLTGLQSLQEKYPIIRDVRGKGLLVGVEFEQPAKGIMAKVAGALNKLASEYLGSMVAG
                     VLLNQYRIITAYTLNNPNVIRLEPPLVVSQEQIDVVLNALDETFHKNRSILGFGLASG
                     KTFLSGMFGKK"
     gene            complement(32814..34112)
                     /locus_tag="VFC74_08835"
     CDS             complement(32814..34112)
                     /locus_tag="VFC74_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010252215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZK25480.1"
                     /translation="MTKVLEHSLLREKISWRRIEPGLEVFVLPKKGYNKKYATYSTNF
                     GSINSRFIVEGQERELQVPDGVAHFLEHKLFEDEQGNAFDRFAVLGASSNAFTNFTST
                     TYLFSATENFTEGLELLLDFVQDPYFTEESVEKEKGIIGQEIRMYEDNPQWRLFFNLL
                     GALYQEHPVRIDIAGTVESISKIDKEVLYQCYRTFYHPSNMAMFVTGDVDAERVLEQI
                     EENIQRHDYQPLGEIKRLFPQEPAEIAKEYVEQTLVVSEPVLNIGFKEQDLGYGGEKL
                     FKREIATNLMLDVVFGPSSKLYNELYEEGLVDDEFDAGYVAEQDYSHSIIGGETAEPE
                     KLFARLVEGIKRAQREGISTETFAHYQRKAMGEFLKSFNSLEFIANNYPAYHFKKINF
                     FDYLQVLHDLTQDEVNQRLQEHLSLDYLARSVIWPKSSPR"
     gene            complement(34112..35386)
                     /locus_tag="VFC74_08840"
     CDS             complement(34112..35386)
                     /locus_tag="VFC74_08840"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HZK25481.1"
                     /translation="MAIFKHKRLENGINVYIHPTAKFKTILVQLFFHRQLLNDEVTQS
                     ALLPAILQRGTGTYPTRQKLALRLEELYGTELMSEVIKKGERQLICYTMDLVHDQFLP
                     EEHQLLRKGLEILHDLLTNPLLAGDTFKADYVEQEKEQHEKVINGLINDKIAYSVERC
                     LQEMCAEEPFSVFKYGSLEQLGKLDATSLFQFYQTFLQESPVDLHIIGDLNVEETFAL
                     VQKIFTYQRGTEKKVPPTKVAVQPREVSYIRDEMDVSQGKLVLGYRTGLHYGDDDYAA
                     LVMYNGILGTFPHSKLFQNVREKASLAYYTYSRLEKHKGLMVISSGIETNNYERTLEI
                     INQQVSDMQKGEFSQADVDNTRSGLRNQYLVEEDHPGALINRTVDGILAERVFDTKDL
                     LARLNAVTRDDIVRIAEKIALDTVYFLTKGGV"
     gene            complement(35499..37664)
                     /locus_tag="VFC74_08845"
     CDS             complement(35499..37664)
                     /locus_tag="VFC74_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25482.1"
                     /translation="MKKALLFSFFCLLLLTNPAQAAAAGKVVIVSLSRTSTQQLADDE
                     NMATWLAQSAVGLLNTGTASNAVSRHLYVTMGAGSRALGNDSTRLAFLQEEEYNGTPA
                     QIIFTRHQGSTASGRIVHVGMADLIYANQTLNYPVQPGLLGDALHAGGKKTAVIGNAD
                     ASQVNREAAAILADSKGEIDFGQVGTAILLDDAAFPYGLRLHKEQVWQIFRELYAKTD
                     VILIDWGDTARLDMYRTQLRQQVAKQLEEEIFRDIDWLLERLTAVLHRDDLLLLLAAV
                     PPNGAAAAETLGLVAVRGGSFLPGRLVTSATTQRAGLAAVTDLAPLVLTQLGLALPQA
                     MIGRPLQLAGSGNVQDLLAMQSTIDRIYRLRSPLLRVYVICQIIFVLGALLNLFLRLI
                     PAKYFESALLGLLSFPLLLLYLPLAQLSLSLSFIVCTAAVVIVVLLLQKLLAGPIERF
                     ALIACATSLSLLIDIFRDAQLMQISVLGYDPVSGARYYGLGNEYMGIWVGTTVLGVTA
                     LTTLAPRWRKWLLGLSTILFAGIALLMVAPNGGANFGGTLTAIAAFITTLAVLFNFRF
                     SWKSASITVVSLVAVAILAILINIYVPQNLQSHLGRTLVLLQQQGWQALKDIIIRKGS
                     MNLKLFRYSQWSRVLLAFLGVLAVLFYRPQGILVDIHKMYPDLAAGFLGIIAGSVTAL
                     LVNDSGVVAAATTLLYAGVPLIILASRVARETPRPIINI"
     gene            complement(37661..38773)
                     /locus_tag="VFC74_08850"
     CDS             complement(37661..38773)
                     /locus_tag="VFC74_08850"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK25483.1"
                     /translation="MEKVHILLVVRPAVGGIREHLRSLITYLAADFTFTLACPQEQQA
                     DYAGLPCQVLPLPLSGKMRPLSDLQAMRQLVRFINTNSVHLLHAHGFKAALVARLAAR
                     ACKRPCLVTVHSDFAQAQAARLPAIYLTAERKLKRWTAGYITVSQWLAEILQQNIQVD
                     EGQISVVPNGIELMADKAAACTLPFSKEAILVGTVARLVPQKGLEVLLQAAALLVPRF
                     PQICFVVAGEGPLCAELEKKRNNLALEQRFFFLGYCEQVAALLARLSVFVLPSLSEAQ
                     GIAVLEAMQAGCPVVVSAAGGLRELIEDNKNGLLVPPGNAVSLAEAIEKLLRQPHLAA
                     RLARQAQADVCRFSISESMRKTKMVYDRVLEGRNTL"
BASE COUNT        10585 a         9477 c         8061 g        10896 t
ORIGIN      
        1 tcatcttatt gtttgtttta aacaaaaaaa ttataggtat aaataattgc aacgaaagat
       61 tgttacccat aatgtcatca tggaaggaaa aagactgctg actgaccaag cctggtaagt
      121 atggattgac aaggaggtaa agggataaaa tcattgtaaa acgttcatat ccgcttttat
      181 ggaagactac acaaaataga ttgacatttt acgcaaaaat tattacaata aatattgtaa
      241 aactaaatat ttcgctttca ggtgccctta taaaagggag aatagggaat taggtgaaaa
      301 tcctaaacgg gcccgccact gtgaagggaa gccggtactg ttaaccactc catagctggg
      361 ggaaggtagt atcgtgtgat gatcctgagt caggagacct gcctgaatat tattaagctg
      421 ttttcgctga tcaagacggc ttattgttaa aatgttggat ctggaaagag gctgggacaa
      481 aactcagtct aaatgaaaga acggttctgt ttatcaaaag aatgatattc agaaccgttt
      541 ttttattgaa taaaaaaaag atataagcat ttgccccttg tacagcggtg tactttctca
      601 agtttaccgc cattaaggca aatcctaatt cattttcaac cttatgtttt ccccgtactg
      661 agaaacgaga aaaacctaaa tttgccttca aagttccaaa aaccggttct acgtcaactt
      721 ttcgtttgcc gtaaatctct ctcggttttt cctctgaaag cttttgtctg atgtattctt
      781 tttgttgttc ccacttctca ttgatataga cttttcggtt tctatcctca gctgcttttg
      841 tacactgatc tcgtaagggg cagcccgtac aattttcaca ttcatacact ttaaaggtcc
      901 gcataaatcc ataccgatct ttgcggtttg aataattctt gaaggttaag ctgcgtttat
      961 taggacatat aaaaatatct tcctttttgt tataatccca gttattaaca ttgaataagt
     1021 tctttttata cgctcttttc ttctctttca catacatatt atatgtaatt agaggagttc
     1081 gcttcctatg attcaataca tggtcataat tttgttcact cccataacca gcgtctgcta
     1141 cgatatactc cggcaaagta aaaaaatgct cctcaatccg gtctaaaaat gggatgaatg
     1201 tccgtgtatc tgtaggatta gcataaatgc catatgctag cgcgtactgg ccttcagtag
     1261 caagctggac attgtatcct gctttcaact gtccgttttt catgtggtca tctttcattc
     1321 gcataaaagt ggcatcatgg tctgtttttg aataactatt tcgattacca aaaatctctt
     1381 cgtcctgtgc atacttttgt ttacgtttta caaaatcaat aaattgcttt cggtattgct
     1441 ttggcgcttt gcgagcagaa cgtaaaattt tacgttctaa cgcatcttca cttttctcaa
     1501 tcttttgtgt gtacttttct acggttttgt caagcttgtc tacaatatta gtaagctcat
     1561 cgatggataa ttcttcatca ctttctcgct caatcgcagg aatgatctgc tcacttaaaa
     1621 gctcttgata tttttcattt gattttttaa taagagcctc actatatttt tctacggatc
     1681 gtttccatgt gaaagtatat ttattggcat ttgcttcgat ctttgtacca tcaataaaaa
     1741 tagcttcttc atcaattatt ttgtcttgga ctagctgaca acgaaactga acaaaacatt
     1801 gatggagcaa gtgcttgaca tcaggactga ctctaaaacg attgatagtt cggtaactag
     1861 gctcgtaacc ttgcgctaac cacatcatac gtacactatc ctgaataagt gcttcaatct
     1921 ttcttcctga aaaaacagat tgagtatagg cacacaagat tagctttaac atcatccgtg
     1981 ggtggtaggc tggacaacct gttttacgtt caaagtttat aaaggcttct tcgggaatgc
     2041 tttcaactag atcatttacc gcgaatgcta catcgttttc ttttagctta atttttaaat
     2101 ctaaaggcaa aactacttga ttcatgttat aatatttata catagggata cctccgattg
     2161 gtttttgtgt agttacttta actttatcag atggtgtccc ttttgtgtat aaaaatattg
     2221 aaaagtaaaa gaaaagtggg aatgcgttcc aagctatgca ttcccacttt tcttgtttat
     2281 tcgctgagtt ttgtcccagc ctctttaatt ttacaaaggt atattaccgt gttttttccg
     2341 aggtctgtct tcctgcttag tggccagcat cgttaaaccg ttaagtaagt agctgcgtgt
     2401 ggcgcggggg tcaataacat catctaccca accgcgggcg gctgtaatat aagggttaga
     2461 gtattttccc ctgtactctt tgatacggga agcacgctcg gcggcaggat catcagctgc
     2521 tgcgatttct ttacggtaaa caacattagc tgctccttcc gggcccatca cagcaatttc
     2581 tgccgccggc caggccaggg ctaagtcacc gcgtacagaa cgggaactca tggcaatata
     2641 ggagccgcca taagctttgc gtaagataac tgaaattttg ggcaccgtcg cttcagagta
     2701 agcatagagc actttggcac catgacgaat aacgccattg tgttcctgct gtacaccagg
     2761 caggtaacct gggacatcca caaaagtaat cagtggaaga ttaaagcagt cacaaaaacg
     2821 gacaaagcgg gcgattttat cggaggagtc aatgtctaag accccggcca tcactttggc
     2881 ttgattagca ataataccta cgctgcgtcc accaaggcgg gcgaaaccga cgacagcgtt
     2941 cttggcaaat gattgatgca cctcgaaaaa ctcactgccg tcgacaacgt gcgtgataat
     3001 ctcccttaca tcatagggtt tattcgcgtt atccggaata atcgtcaaca tttcttctgc
     3061 tgcctgcaat ggttcgctgc ttgctactcg tggtggatct tccaaattat tcgccggcag
     3121 atatgataaa agctgacgca gcttaataaa gcagtctgct tcattactgg ctgcaaagtg
     3181 ggccacacca cttgtttcat tatggcgctt ggcaccacct agtgcttctg atgtgacatc
     3241 ctcaccggtc actgctttaa taacttgtgg gccagtaata aacatctggc tagtctgctc
     3301 gaccataaaa ataaagtcag taattgccgg tgaataaaca gcaccgccgg cacaaggacc
     3361 cataattaca gaaatctgcg gaatgacgcc gctggccagg gtattacgat aaaatatttc
     3421 accaaaaccg tttagtgctt ggacaccttc ctggatccgt gccccgccgg aatcatttaa
     3481 cccgatacag ggtacaccag ttttcatggc caaatccatc accttacaga ttttggctgc
     3541 gtgcatttca cctagcgaac cgccagccac ggtaaagtct tgtgcatata tgtagaccgg
     3601 acgaccgtta atcgtaccaa agcctgtaac tacaccctca ccaggattgc tgtgccctac
     3661 tttgccggca tcttccaagc ctgactggac aaaaacatct aattcggtaa aactgccggc
     3721 atctaataac gcctgcaagc gttcacgcgc ggtttgctta cctttttcat gctgggcagc
     3781 aatccgtttt tcaccgccgc cgagagcaat taatgctttt tgttcttgta ataacttgat
     3841 gctatcttgc accttcactc ctcctcacca tcttaaaaag attctttagt gattatacac
     3901 ttgcgttagc taaattacaa tttctgtcgt taaaaattga cattagcaat caataagctt
     3961 tacctgttag actgatactt ttttaattta ttgtaaagtg ttgccagtga aatattaagg
     4021 gctttagcag cttttttctt gccctccagt gaattaccaa agcgtgacag cgcggctttt
     4081 aacatgattt gctccatctc agccagagga ataatctctg taacctgggg cggtcgctct
     4141 tcataaaact gactaatgta tggtgataaa tgctgacact ttaatctctt attttcaacc
     4201 aaaaacattg cccgctcaat tatattttct aactgcaaaa cattgcccgg ccaatcataa
     4261 tcaactaaga gctgcaatgc ttcaggctct attaccgtca catgcttgtc aaacttgcgg
     4321 ttatagacat tgataaagtg ttttgctaaa acagggatat cctcacgccg ttggcgcaat
     4381 ggcggcagtt taattgtaaa caccttcagc aaagcatata attctgcaga gaaaaaacct
     4441 ttcgccgcca gctcttttaa gtctctttta gtggaggcaa taatacggac atcggcgtgc
     4501 tgcacctctt cactacccaa acgctgaaac tcaccttcca gcagcagcct caccagtttc
     4561 tgctgtaaat ctaggcttaa accggcaatg tcctcaagat aaatggtgcc gcctttgacc
     4621 atttccactt taccaagcac cgtttttcct gcaccagcaa aagcacctct ttcatgacca
     4681 aacagttcac tttccaagag ctctttggcg atcgccgtcg tactcacttt gagataggag
     4741 ttgtggtgcc gcaggctggc acgatgaata gcctgcgcaa atacttcctt gcctgtacct
     4801 ccttcaccaa ccagtaaaat cggcaaatta ctcctcaccg ctcttttggc actgtccaca
     4861 gtagagctaa ataatttact tttacctttt aaatcctcga aagtaaactt agcgttagtt
     4921 atttgatgaa tgcgatcgta cagattctca ataatcgtgg tacttttacg taaatctttc
     4981 atcagcttca gcagattagt gataggctgg cagacagcaa cagccccatc tagctcaccg
     5041 tctaccataa taggcgcagt attggccaag acatccacat tttcaatttt agtacggaaa
     5101 ttggtcagag ctctattcag cattaatgtt tgggctaagg cgccatgcgg gttaacttca
     5161 aagatattac tccccacttt ttgcttactg ttaacacctg tcatctgtgc aaaagaagga
     5221 ttaaaatatt ttaagacacc attactatca gcaatggcca cgccttcctg catcgaattc
     5281 aaaataaccc aaagctcact ttttaatttc ttttcatcaa tttttgattt aatgatggcc
     5341 atgagtaagg aatcggccgg ggcagctaaa acttgtgtct tagctgcttt taactgtttt
     5401 atttgctgca agatgtctac gtcaccagtt aagtcaataa tcacatccaa atcaggttta
     5461 gtcaccaacg ctgctaactc tgcagtcaca tcaagctggt ctcgttgtgc cataatcatg
     5521 ccgagcgcat ttaagttttg ccccattacc cccatgattt gtacatgctc aatttcacgt
     5581 aaaacccgat ataacgaggt atctgcatta tcagctccaa tgagcacaac tttaatgatt
     5641 ggcaactatc tcagctccag ttgaggtatt ttttatgcga acccgattta catcagaagt
     5701 agttgacttt tcatcctgcc aacaagcatc tgccattaag tgaagttttc cctaggtgcg
     5761 gtgagcacta aatctatgtt ataatgacca ataacgatgt taggggagga aattatgcaa
     5821 gtatatgcat taattggggc tagtggtact ggaaaatcac atagagcttc ctttttggct
     5881 tttaaatatg aaattcccct gattattgat gatgggctgc tcattgccgg cacctctgtg
     5941 ctggctggca aatcagccaa acgtgaaaca actcgttacg gagctgtaaa acgcgctatt
     6001 ttttatgacg ataagcacgc acaggaagta caagagaaaa taaaggctag tggtgcagag
     6061 agtattttag tgctgggtac ttcagataaa atggtactac agataacaaa acgcttggat
     6121 ttgcctgcac cggataaaat catccgcatt gaagagattg cctcccaacg cgccatcagc
     6181 aaggccttgg aactgcggca gaaagaaaat cgccatgtga tacctatccc cacctttgcc
     6241 atcaaaaagg agttcccggg ctatttaatt gatcccttac gctctttttg ggggaaaaac
     6301 cgtaacgata gcaaaaaagt agtaattgaa cgttcgattg ttcgtcctat ttacaactca
     6361 tttggtagta attatatttc tgaacaagtg ataatgcaaa tggtaacaca tattgccgcc
     6421 aaattgaacg gtgtctcccg tgtcgggaaa gtagaagttc tttctaaacc agagggcaca
     6481 gttaacctcc aagtggaagt taatgtgttt tacggacact ttatccctga agttttgcag
     6541 cacattcagt ctgtcctgaa agaggaaatg gaatatttaa caggcttgac aattaataat
     6601 atccaggtca ccgcccgtcg gatagattta ggtactaact tccagcttca atagccgcaa
     6661 taataccaga cagtcgcaaa caaaaggctt ggcatcatcg attagatgcc aagcctttcg
     6721 tttgcttaat ttagcataaa gccttaatta tttccacagc tgcctgcacc tctgtcagac
     6781 taacatcacg atgcgttaca aagcgtacgg cagcagaacc agaagttcca gccaatacac
     6841 cctgctgctg cattttttgc agaaaatcat gcactaacat ctgttctaat tctaccatca
     6901 ccatatttgt ctgcacttta gtcagatcta cttttaggcc tggtacaacg gccaatgctt
     6961 gcgccagata ctgagccttc tcatgatcag ccattagaaa agaagtatcc tctaaagcaa
     7021 agatacctgc agctgccaaa atgccagcct gcctcatgcc cccacccagc attttacggt
     7081 tccttcttgc ctggtcaatg aactgcctgc tgccggcaag gatagaacca acgggagcac
     7141 caagcccctt agaaagacaa aacatcaccg aatcggcatg agcagttatg tcagcaggtt
     7201 cacagcctag agctaaagcc gcattaaaaa tacgtgcgcc gtctaaatgc acattcagtc
     7261 cgtattcctt ggcaaggcga taaacttcag ccatttgctc tggcttcatc actgtgccgc
     7321 caccacggtt taaggtattt tccagacata ccagacgagt cctcgggaaa aagtcctggg
     7381 gattgcgaat atgctcgctt aagttggcaa ttcctgcctc attatgcaaa tcgtccagcg
     7441 gatgcagctg cacaccggaa agcgtcgctg ccgccgccat ttcataatgg aaaacatgcg
     7501 ctaatgaatc acaaattgct tcgaccccac gctcacagtg cgtcataatt gccaactgat
     7561 tacccattga gcccgatggt acaaagagcg ctgcttcttt actcatcact gctgcggcca
     7621 tttcttctaa acgattaatt gtgggatctt cgccgtaaac atcatctccc acaattgctt
     7681 ctgccatcgc ctgacgcatg gcctgcgtcg gcttggtaac tgtgtcactt cgcatgtcga
     7741 ttattttcat catacgtcct cccccaattt gctccttaaa ttagaactct acgctgatgc
     7801 gaataaatat tacagtgtct cccccgggca aaaccaatta aagtaatacc taaattctct
     7861 gctaattcta tacttaaagt cgtcggtgcg gcgcgggaca ccagcaccgg gatgcccagt
     7921 ttagctgttt ttactagaat ctctgaagag attctgccgc tgctcacaag caccttgtct
     7981 gccagactaa tcttttgctt taaacatagc ccaattaact tgtccacggc attgtgacgg
     8041 ccaatatctt cacaaaagta tagtatttct tcagcagagc ataaggctgc gctgtgtaaa
     8101 ccgcccgtta atttaaacaa ctctgccctc acctgcagtt gtttcatcaa ctgcagcact
     8161 tgaaaaacag acaaagaaaa atctgactgc acaggactac tctttaacgc atccaacaca
     8221 ctgtaaaaaa ctgtcccttt tccacaacca ctggtgatcg tccgtttacc gtacaatttt
     8281 tctgttagtt tgctaccctc tgtctccaca aaaataaaac tctgctgttc atcgtagcgc
     8341 agagaaataa tatccctata atcgcgaatc aaaccctcag aacgtaaaaa tcccaccgcc
     8401 agcatctccg gcagttctgg cgtataaagt aacgtcacaa actcttggcc attcagatag
     8461 atcgtcagag gcacttccct gaccacagga tcatcaaacg actcctgccg ctcaccattg
     8521 atccgcaaaa tcggccgcat ttccatccgg ctatccatct catccctcct tagggtcgat
     8581 agcttgtccc tggagcagga caacatacag taattgtatc ctcaagtcag ctgtaattca
     8641 agggaaaagt cagatcaaag cggtcaaata agctacataa attgttagac ttttttcatc
     8701 accttcagac aggtttgtca taattaacat tcagcaatag ttattctgtc ttctctatac
     8761 aaaaatatag caaagattag tattcacgtt ttatgtaatt gcttatatta tttattacat
     8821 tattaagtat agctgaaaag cacaatttcg ccacaatttc atattatata ttgagaattc
     8881 tcatagtaaa taaaggagtg acttaatgat gagtgtaagt aaagctaacc acacccgcta
     8941 ccccagtaaa atctgtcatg gttcgagttg tcataagccc cataagacta gtgatattat
     9001 tttagaatgt gggtgcagtc cggaagctgc atttttcgaa attgatgatg gtagggttga
     9061 agaagggcaa aagtttgttt tggatcgtgt gagaatagat attactcact taataaaacc
     9121 agttattaaa ctcgattttt caagcctgat aatctttgaa gcagaagatg aagagggcag
     9181 tgaacatgaa gtagaagttg acttgctatt taagttgatc agaattagca atggagaaaa
     9241 agaagttatt cgaagctgga gctatctgta tgaaattgat gttgaaaata atattgatga
     9301 gctggaagta gaaatgaaac agccgtttgc agtaacattc tgtgacaaac catgcgcaac
     9361 tgtctgtgaa tatattatga aagtagaagg cattgacttc gagggagatt ttgatgaatt
     9421 gagagtagta agtcctactt taagtgcgat tgcacaaggt cagtgttagc ttattattta
     9481 accatttctg attaagaatc ggggggtgtt gcaaaactac tcaaaagagt agctagcaac
     9541 accacttttt tctctaaaac cagcactttt ttacgtttga ggcaggcaat atttcataaa
     9601 taaaaagtct ctgacaaagg gaaaacccaa tctattcagt atctttctcc tgctgcagaa
     9661 tctcgctgcc tgcttcttta agcatattca catagaccag cagctcgtct cgcgaggcaa
     9721 cattgagttt catatagatt cgtttattat gcgttttgat ggtgttaatg cttaaattga
     9781 gaattcccgt aatttccttg gccgtatgtc cctctacata aaggttaaat actgctcgtt
     9841 ccgcaggcga aagtaattcc gtgtttttcg caaacatctc ataggcggaa gtgggaaggt
     9901 ttatattttc accctgcgtg aggagaggct gagttacttc ctgatgtgag gagagaaact
     9961 ccactaaatc atcgatctca acaacttttg ttttagctgc ttcactaaaa tcattggact
    10021 tgatcatctc catgctttca gcaaagggct ttaaatatcg tttgctgagc agataggaaa
    10081 taatgctgcc tatcgctagc aggagaatta agagcagggt tagccgcaaa ttggccttga
    10141 tgaaggcagc agaaatatct tcatcgggaa gcatcactgc taccgcccat ttttctgccg
    10201 caaaagctga accttcggga taaagattca actgtcgatg gagaccgcta tacaaggttc
    10261 cgtcatccag ccgatacgac ataaaaggat gaccttcctt aatcgtaagt gtttggtcgg
    10321 tttttgcacg gttgcgggga gagtaactac cggctgaaaa ggcttgggag atatttagtg
    10381 tatcctctga agtgggagat aaaacactga agacacgctg ataggtatca atgtttggcg
    10441 tatgagatag cttgaacaac attgcactta cctcaaatcc acaaacccca aaaatatgtc
    10501 ctttggaatt gatgaggggc acagaacaga gcatgatttc ctcgtttgcc cccggcaagg
    10561 ttatcacatc gctccaataa tataaccgtg agagcgctag ctcatgatcg gtcgcttcct
    10621 tgattgggcg gcgatagtag ggtgcgttcg tgacatcaaa ctccattctc cattgcgaat
    10681 gaagcgtaat tgcattatca cggccaatgc tgggaaagcc gcgtaagaca aaaagataag
    10741 cagcagttga actaagaata ttaggttcca tattcttgat gtataaacca gcttttgaat
    10801 tttcagcctt ttctaaagct gggttaaccg tggcatccaa gatcataaag acgccactgc
    10861 tttttgccct ttgtaaggcc gacagactag cggcgtattc tccgccaact atttcctcta
    10921 gaagttcagg gtggttcttt aaatcgctgg ttgaaagtcc acgcatcttt aatttcattt
    10981 caatgctggc ggacaacact ttagaaagct cgatagcatg tgcagaatat cgaacatatt
    11041 ggtcgctgat atcttgtgaa atataagcca actctttttc aacgtctttt tgaacttctc
    11101 cgacgccagt gttaagcgtt cccgtgatca aaagaaggag aaatatactt aaaaccatga
    11161 aaaaggccag cgtgattaaa aatagaaaca gtttaagtct catcgagatg ctcagactgt
    11221 gtacactatg tttttccaac ctcacaacca ccttattcta ccagtttttc tgcctataaa
    11281 aataacatct tctacttaag caacagcagt atttagtaag cggttattca gcggacaaaa
    11341 acctcaaaaa ccccttgtga cgcttcgtta aaggcggcat ctctgctcat tttggcaagt
    11401 aatgtctgat atgtttttct ggattcagtc gccgcttgct tcaactttgt ctcataatct
    11461 ttttgcatga catcgacgcc ctcatagatt ggcggaatat aaaaatcata ttgggactgc
    11521 atttcaacta ctgaagtcag cagtgtttgg atgttcttat cctcaattac cgctatttcc
    11581 tcagccatga tctcgccaaa ggcactttct gtaacgggaa gatagccggt ggtggctaca
    11641 aagcgtagat tgttctgcgg acttgtaaac catttgagga aaacacctgc agcatattcc
    11701 ttggtcttag tcgactttgt aacaagcata ccactgccgc gctgtatggc tatctttctg
    11761 cctccctcga agatcggata gggtaagatc attaactcaa cgggttcagt agtattatcc
    11821 gcataggtaa tcagatgatc aaaaaacagt actcctgcgg ttgagccgat agaacaggtg
    11881 atatcacctg tcttcgctag gtctgaggca tagccgtcat agatggcaaa gtatccgcgt
    11941 actgctgctt cataatataa gttccagacc cgctgatatg cttcggatga atcgtccaat
    12001 tgacctcgca caatgaaatc ataccctaac tgcttgcctc ccacttgagt atagttgaaa
    12061 agcgagtcca tatgaaagaa ggccttacca tcatttaaag ttccgggtgt ctgggcgtct
    12121 gtccattcgt aataaagttt tgctgtttta gcgagacctt caaaggtttg caggtcttca
    12181 tgactagcac ctgttgcttc gctgaaacga ttaaaaatcg ttttatttaa aaagagcact
    12241 tctgtagatt ttgctgtagg gaaaagatat aaattctcgc cttctaatct cccttcttcc
    12301 aaaaaacgag gcacgtaagc ggtgagttcc tcttcagtaa actgctcagc caagttagtg
    12361 agtaaccctt tttcagctag aataacggca gttttgggat tagctgtggt tagatctggc
    12421 atctctgggg tccctggctc acggttggca gccataatta gtttttcatg cagagcagag
    12481 gaactggaga tggaagttac ggagagaata atcccctttt cagcaccaac agtcgcatta
    12541 aattcatcaa ccatcttatc catagtatct ttcatctggc caccgtagtt atgccacagc
    12601 gttagtggca caggtttatt agcatcgagt ccatggttgt tttgacatcc cgacagcata
    12661 gcaaaaatca tcatactgac aattaaaacc gcaatctgtt ttctaatttt caacgccccc
    12721 tgccgtttag taaagatcac ccctaaaatc acccctgctt cggtggttta gtcatccttt
    12781 aggtgattac aaaaaaaaag ctggtagcta taatgtagaa gtgagatgct ttggcatcga
    12841 caacgcagaa gaaagctggc ttcactagcc attgtattcg ctgctactat aaaaaaagtg
    12901 cttttacaga atttagtctc gtaagctctg agcggaggca gtatactaat ttaaggaggt
    12961 ttctctggtg acagacgaca gtggaaaaaa tggccgccct gaacagccct ttgctcctgc
    13021 cgacattgag caacacaaaa ccatggctgg actcgcctat gccatctgct tcctacccct
    13081 gattgcctgc aaagattctc cctatgggag gtttcatgct aatcaaggtt tattgctcct
    13141 gctgctaatc atggtgggga gtattgtgct ctctgtggtg tctagtatcc tcattgccct
    13201 tacctggagg ctcttatggc tttcctccct gctgtacggt atctttagct tgctgatcat
    13261 ttatttgctg ctgctggggc taatcaatgg acttaacggc aagccaaaac gactccccct
    13321 tatcggtgga attcaaatta taaaataatg aaagtgggtt cattgatgaa aaaactaata
    13381 tcttattttt taattttact gctgatcagt tcattcgtta ttggttgcga aaatacctcc
    13441 caaacagaag acaaaactcc aaattcaaac acgcaaaaca gtaatgatgg cacctctgcc
    13501 gaaaaacctc ctgctaattc agacggcatt ttaggtgcta atctatctac cgcctatgcg
    13561 gagatgatga gaaatgacaa atatttaatg agatatacaa tgacttcgga atatgatggg
    13621 caagcgctgg agttcgagat caccgttggc gtagcgggag ataactcagc agtgaccaac
    13681 atcggagaag ggatgaatac ctcagttatt tccaaaaacg actcaaccta tatgattgac
    13741 catctgcaaa aaacagtcct ggaaataccc gccgacagca tgatgagcgg cgagatggac
    13801 gataatcaaa tcgggaccga agaattaacc tacgaaagca gcggccaaga agatggcttg
    13861 gcctatgaga aatatactac ggctgatagt atccttatct attattttga gggtaaaaaa
    13921 ctagttaaga ttgtcattga ggttgatgag gaaccagtag tgatgaatat tctggagatg
    13981 agcaacaccg tacctgacag catctttgaa atccctgcag actacgccaa gaccacacta
    14041 tctgattaat tagcagtatc aactctctga taattgtgca aagtttcagc tcctggcaaa
    14101 acaaacatac ctaaggagga ttgcaccatg actgacaata tatttggtga ccttgtaaaa
    14161 agccttggtt cttttatgcc caatgatgat ccagatatca agctttttca agcacaaaca
    14221 gaaataagta gtctcgagca tagagaacag gagttgttcg gggaaattgg cagaaaagag
    14281 tttccccaca tttgtgagca gccggaatac agcgagatag tgcaagagct tgcgacatta
    14341 caaaaaaaac tccaaacggc gcgctccacg ctgcaaaagg ttcaatccga aaaagcggag
    14401 caagatcgtt tggaaaaaga aaaactactc agtcgtacct gctcccactg tgatacagta
    14461 aatccggaag gagttcaatt ttgtcaggaa tgtggtgcaa agctgggaag cagtaataaa
    14521 ccggtctgcc cccaatgcag tacggagtat caagcaggta ctcgattttg cgggcattgt
    14581 gggagcaagc tttaaacttt aaaaacagga gtgttgccca tggcatctta caaacattca
    14641 tgtatccatt gcggggcaat gattgcctcg gctgctcgct tttgtcctaa atgtggaagt
    14701 attagtcctt tcgccctacg ctgcccctcc tgcctcaagg aagttagcaa gggggatctg
    14761 ctctgcgcag gctgtggcag accccttgtc atcccctgtc cccaatgtgg caaggatacc
    14821 ttcctcctcg agaaatgtga agtttgtgct gccagcctga tggtgaaatg ccctaatcag
    14881 aggtgcgaag aaatggtctt ttttcaaaac acccactgtc gggcttgcgg tcaaaaaatt
    14941 aaaaaataag cactagttcc tttgctttac tgacattgtg agacaagggc ttatgcgaat
    15001 ctatgaggtg atatagtgct aacagctttt acaagaaatt atgaagataa ttcaacagag
    15061 gccggctttc aatttacctt ctattgtgat ctatgtcatg atgggtataa atcctctttt
    15121 atcgaatcag aaacgtataa aaaggggtct ttactgcgtg gaatttctgg tggcgcccgg
    15181 gcacttggcg gtttgttcgg tggtaaattg agtgatctgg cctggagtgc agggtatggc
    15241 ggtgatatct tatccgaaaa gttcaatggc atgagtccag aatggcaaaa agagcatgaa
    15301 aaggcctttg aaagagctca gaacgaagca caatttcatt ttcaccgctg ccataactgc
    15361 cgtttatgga tctgcgatgc agacttcaac gaagatgagg gcctgtgcgt agaatgtgca
    15421 cccagaatga atattgaaat atctacagcc agagcgaaaa aaatggttca ggacatcgaa
    15481 gaaaaagcac aggccagttc ggtatttgac ggtaagatcg aagccaaaac catcatctgc
    15541 cctgcctgcg gcaagccgag tggaacaggt aagttctgta ataattgtgg cacaaacctc
    15601 tccctgctca aatgtccctc ctgtggttct aatcttgccc aaggcgtgaa gttctgcggc
    15661 gaatgtggtc ataaaatgag ctaaccccaa gtcccaagat gatcagcttc agtaatagtg
    15721 cacctgcttt tttcatctac aagatgagaa aagcaggtgc actatttttt gttcccgtta
    15781 tagctgaaat tagccagtag ctttaatcct agtctacgcc aaaatatgcc tctactgctt
    15841 tcttgtaacc caagccaaaa ctcggctgga tttcctgtgc ttcacatgat tgaaggagct
    15901 tcgacaaagc gtccccaata tcttttcctg gtatatacaa ctcgccgaaa gcctgccctg
    15961 cccgattgat gtgaccaata gtaccagtag catcactaat cgttttcatc cgttctccaa
    16021 ttataccaca tagtttcgcg gcattggctc tgatttcctt ataatcatcg aggacaaggg
    16081 cgaaggtgga aaccccccac atagctgttg taaccccagt aatcacacct accccaactt
    16141 ccatgatgtt ttcggggaaa aggccgatta gataatctgc catccctgca cgctctccgc
    16201 tttcaaatac ggaaaccaca tcgtaagttg tctggttaat ctccagccat gcccagcggg
    16261 gtttaccgtc aatggcaact gggtaatcag gtatgatata taaggttccc tccatggcac
    16321 cgtcctctat tttagcggtg aggcgcttga caagtagtgg tggataacca ttttcgctca
    16381 gttcctgtgc tgacgcttca tactgtccag cttccgtggg tgccataata tacatacttg
    16441 cgtccttcgg cagcccgttc caaacatcca gatagctgat tccctcacta ttgggcagtg
    16501 cagaagcttc ggcttcagag gcaaaatagc cgcacatcaa gttataggcc cactgcttct
    16561 cctcttcacc acggtgtaat tggttgcggt gattcattaa gtccacgacg gcttcggcct
    16621 ttttagtatc gcttttcatg gtgacaatca gagcaattgg tgcagtgatt cgtcccgctg
    16681 tcacttcaag ttttttagcg gtttcgcggc caaagtcggt aaagttcttg acaaaacgac
    16741 tgattgttgc gtgtcccagc caacgaccga tcgaataatt tgtaggatct ttcgctgctt
    16801 tcgcctctgc ttccaccatt ttttcatacg gctgtgccgc ttcgctgctc atctctggga
    16861 tcccccaagc cagggtatgg catacttctg ttattttttg atcttttccc aggattgtgg
    16921 tgtgtacaac ttgatcattg atatctgtgc ctagtttgat ggaaatagat tcgaattcat
    16981 aattagagct atctacccaa ccggcttcat cgaacaacag cccctgtttt gtatcaagta
    17041 ctggcaggat aagctccccg ccctcacttt tagcggtggc aatataacta atatcgatcg
    17101 gatcaaggct catatccggc aaggaaacct ctctctcaaa gacagtaaca gtctcataaa
    17161 agtcgctgcc attctccccg ccgcccagtg ctgcggcaaa gtcaccaaac attccttgtg
    17221 tcgccgccat accagcgctg cccacaagct tgccggtcat ttctataata atcttagact
    17281 tagctggcga gatttcccct aaatccacat cgctgctgat ttcacatagg ccgtaaagct
    17341 ccgagatatc acgggaaaac actggcacat aaattcttaa ttcacctttg gcatcggtat
    17401 aggaaaccgc cggcagccag gtgggaatct cctcgacagc accgagacgc tgcaggtttt
    17461 ccttacccac agttgttagc ttcacctggc tgtgctgcgg ccggtagcca agccgggaga
    17521 gcatggtgtg ggccaaaatt gctagatcat actgacttcc ccatccctgg gtaagcaccg
    17581 cttctggtgc atagtagtaa tgggctatgt ctgtgctcgg cacacacttc tggccattca
    17641 tcacactcaa gaagtcttca tcagttctca cgctttgcaa gagttgactt ccatagagcg
    17701 tgagagaagg ggccggaacc tgctcaccta aaatctcggc atccgacagc cccacccgtg
    17761 tggtcagatt cggctcctgg cgggttgagt gatacttggt gacagcagtg gtcaccgctt
    17821 cattgaggat gttttcaaat tcgctgttga tggggatttc tggggtctta gccaacagcg
    17881 cctgatatgg atacacgctc tcgctcaccg gaacctcaag gtcacggagc gctgtactcg
    17941 ttagcgtcca atctgcaggg gcggactcat acggaagggt aaataccagc agcccgcgtc
    18001 gcttttgccc atcgaaggct ccccagttgc cgacagcatt atatgcccca tacaggagca
    18061 tcgctgtttg ctccacatta accgccacaa tgccctcgtt ttcttcagag tcatctgcaa
    18121 agttgccaag tcccttacgt gccccggcaa tccgtttgag ttcctcagcg ttaagttccc
    18181 taacctcccc tgggagaggc ttggtgattt cactgcttct ttgcagctgc agtacacctg
    18241 ccagcccacc tgtaccggca ccatcttttt tgtcctcgat ggtgaagtcg agcactagat
    18301 gattactgtc aggctccagg cgtgacagag agttaatctt caggttaaaa tattcatgtg
    18361 caaaggtttt gccgatacta ccagtaacat aggggctgcc ttccgttaca gggatagtaa
    18421 gagagccctc ctgcaaatct ccaaaaatac ttgattttac ttttaaaagc tcttggggaa
    18481 cgaaaaacgg taagcgcaca ttattgcacg atcccggtgc aagcattgtt ttacccgtga
    18541 agcccaaggg cagattatag acgatattgc tcatctcggt caatagtaca ccttgatcag
    18601 tctccaactg atgtaaaagt cgtttttggg ggtcgatatc taaaagtgcc tgtacatggc
    18661 tgtcaaagcg cgcctccaat actcgaaagc tcacatcctt ttgtttatta gcagatgtgt
    18721 cgtcggactc tgcctgtgct attgaaacgc cctcaagcgt agtaaggtcc ttcttgccgg
    18781 taatggcaag tgagaaggcc tcgctgattt tgttagcagc ggttgtcggc agactatcca
    18841 ggtttactag gtttcgtgct ctccccccag tcagtgacaa ctggatatgt ccattgacgg
    18901 tgtcatacat atgcagggac agctcgccga cattttctcc cgtgcccatg tctgctaaaa
    18961 acaccagcat gccgctgcgt ttttcccctt tcatcaccgt aacctcaggg tctcccggga
    19021 gggcaaatgg tgtttctgcc agccaggttg cctctgaagc tggcatcatc tgcccttcat
    19081 tataggagac gtagaagtgg ttgaagatat tagggattct atatcctttt tcaggatcag
    19141 cagagtttgc tgctttgaaa gtaagttctg tgtttagagc cagaaagcat tttccgctgg
    19201 gaggggtgat ccctttaaat agatccagta cataggcttc agatactgtg aggctgccga
    19261 agttattggc ggcagtctta tcttttaacg gcactctaaa tagcacacga ctatccacat
    19321 tcggcagatc gctgccatac agcagctgcg ccgcttgccg atcatagtta acgagcttct
    19381 cccctgtcgc catagtaacc ataccgggtt ttaacgtctc gccaagctta ctcttcagcg
    19441 gagaaaagtt ttctaggttg attcccgaat gaagattgag gcggctgccg tcagcggtgc
    19501 tgcagtcaat ctggattgta aaatcaatgc cgtctttggg agtgggttca tcaatccgtt
    19561 tcaaaagatc agcgagttta gccgctatat cctcgacatt gttcgtaaca atatagtccc
    19621 cgccagctac ttgggcagcg gcctggaagg cagcctcata agcagctgca tttgtagtgt
    19681 ttagcaagcc tgcaatcagg accctaatgc cgctattttt cagttccgcg agcacctcat
    19741 cgatcagctg cttttcaatc tcgtcgccac ctgaaattgc ggcatcggta aagaagacta
    19801 atacccgctt gctgctcggt acggtagaaa ggttttgctg ggcaagctgg agtgccgtga
    19861 tcgtcggtgt tcctcctcca gccacaagac tgccaatcgc ctgtaaagcg ggcgcctgct
    19921 tgtctgttgt gatctgatcg taacaaggtt cagccccgcc caatggttcg gcaaagctgc
    19981 ccacctgcat caaggtacct ttaggcagac cgagaataaa atcatggaaa atcgccttga
    20041 cgccatccaa gcgccagcca acatcactgt caggatcctc cgcagggtcg tcatccatcg
    20101 aaccggagcg gtcaatcatc agtgacacaa cgggcaccgc actactttcg tcagctgtga
    20161 cggtgggacg ttgataatct atagtaaact gattgcctaa cttgccggca accgaggcaa
    20221 aaacaccgtc caaggcgttt ttatctccgg cagcaaaata ggcaccgttg gcagatgatt
    20281 ttgccatctg caccagggta gcaggatcat gccccttgcc gaagccgatg gtgagaattg
    20341 tcacgccgct ggccttgatt tgtccgagca gcttatcctt tgtcagcgcg ctcccagtac
    20401 catcaactcc cggttcacga gaatccgcgc catcggaaaa gaccacagca tagcctctct
    20461 ttttaccttt cagcagcccc atggcgcgac tggtggcatc gtataaggat gtcgcgccgc
    20521 ctgctttaat cgtcgccaaa gattttaaaa cagctgcttt tcctgcacct tgatgctctg
    20581 tgattttctg agcaaactga atcaagcgga tattggagtt ttcgggaagc ccgctgacaa
    20641 aacgtttagc cgcttcaatg caaggctgca tattctcccc catggaaccg gaggaatcaa
    20701 gtaccaacac cacgtctgat ccggctggct cacgggtgat gttggtaata acggcatcaa
    20761 ccccattctc tgtaatggca acgtcctctt tattcaggga aatatccctg tcaaggggat
    20821 cattaagcac cagagaaacg gaagcggtag ctccgttgtc tttattcgtt aaaatttcga
    20881 tcccgccctc attgaggtca aaatcaccgt acttccgtga catcgcgcct actgcatact
    20941 ccatttcctg tgggatcgtc accgtagtga cctccccggc gctaactggg acaagacgaa
    21001 ggaaagaaag ctcatcccct agaccagagg gaaatgagac attgtagtaa ccagctggca
    21061 cgttaaagag catagtgcca ttttgttcgg tgacactaaa tgcattgttc tccccaagag
    21121 cgctatgggt aatggttgcc caattcggct gctggactcc agccatgccc aggggtatcc
    21181 ccgtcacgcg gatggcgccg ctcgcttctc cccattttac tttttcgatt tcagccacat
    21241 tatcaatgca cactgtgatt tcctcgggaa gctgttctga agacgccgta aatttatagc
    21301 acaacatccc ctctgccagg ggtaaatcgt caaacacaaa cttattgccc tttttcgtat
    21361 ttagttcatc agaggagatt actttgctga cattatattg atacgagcca tagttgacag
    21421 cctgttcaac tctgaactca accccgctgt ttgcgtctcc ttttagtgat attgtggccg
    21481 attgcagctg gcccggcgta cattctgtat agaaatagta ttcgccctct tcagccttgt
    21541 tgatctgctc cctgctgatg gtcatctctt ttcccaaagg aagggtagat atttttacca
    21601 catgcaggtc tgcggcttct tcagagattt gggcatcaac ccaccatgtg gtgtgatcat
    21661 tttccttaac cgaaaaaaca ctgtgctctg ccgtgctgcc atagcgggtg gccccgagct
    21721 tgttggcata ccagcgcagc cacaaaattg aaatctcact ctcaagtgga atagacatat
    21781 tccacacctc acccctcacg gcctgagttt tgggtgtcaa cccctcgagg gtgtgcagca
    21841 gcagttcttc gctgttttcc acctgctcta caatactata taacgaatac gaacccaatg
    21901 gattgaaata tggaatagca agatcacccc agtcgtcagc agaacccgtc tcgatttcta
    21961 caaagtccac accaggtttg ccaatgccat cttcaggcat cttattgaga ccggttaatt
    22021 ctggggcggc cactgcgctg atttgttcca acgggacttt ttcgccggca acctcatctt
    22081 gatttccgcc gcagcctgct aacgggacaa ccaccagtgc aatcaccagc agataggcga
    22141 taattttttt aaacaaactc catacctcct cttccattgt ctgtatttgt cattctgcat
    22201 tgtgcatcta aaaaagagtg atctccctgt caccatgcga tcacccctga aaaacttaca
    22261 tacttccatt atgtacgata atgttctccc agtaatcacc cgaaggatga aaaaactatc
    22321 aaaatagggg tgattttggg ggtgatcttc tgaaatgata caaaaaaatc attactgccg
    22381 accttcttgc agcagtaatg atttttaaca agttgaaagg gttctagcaa cataattaag
    22441 ctgatgtgac gaataatcaa agagaagcga aaacgttttg ttgtccttta ggtaaagcta
    22501 gtgccacagg caacaataaa agcgggtgct tttcgcaccc gctttggttt gagtttatgc
    22561 ttttacgatc ttgacagcac aagccttaag ctcggggatg ccggccgtcg gacatgtttc
    22621 tgggttagta agcaggttag ctgctgtctc tttgaagtgg aaggtcatga aaacgacgcc
    22681 tttggggacc gtatcggtga tatttaggcg ggagttcact gaaccgcggc gtgaggtgac
    22741 cttgacagtg tcacactctg tcacccctaa ggcttgagca tcagcaggat tgatttgcat
    22801 caattcctca tcttgcataa acttcaagcc tttgctacga cgtgacatcg taccggtatg
    22861 gtagtgataa agaatccgcc ctgttgtcaa cataaaggga tattcttgat caggaacttc
    22921 gtctggacct tgatgttcca caccactaaa gtgacctaaa ccacgactga acttgccgat
    22981 atgcagaatg ggtgtgccgg gatgttctgc cgtggggcag ggccaatgga taccgctaac
    23041 gcctaaacga tcatagctga taccggcata gctaggtgtc agtgcagcaa tttcctggaa
    23101 gatatcctct gcacaagtat agtcccaagc caagcccagt ttattagcca gctcaactgt
    23161 gatctgccag tcagccttag cgttgccacg cggttttaac gctggattta ctttttgcac
    23221 gcgacgttct gtattagaaa aagtgccttc tttttctgcc caagaaacgg caggcagcac
    23281 aacgtcagca tattttgctg tttccgtcat aaagatgtct tgcaccacca ggaagtctaa
    23341 agtctccatg cagtgaatta cgtgtgatga atctggatct gacagatatg ggttctcacc
    23401 catgatatac atcgcacgta cttctttagc tcctgcagca tcaaacatct cggaaacagt
    23461 taagccagct ttaccagaga gttccctacc ccaggcctta gcaaatttag cacgtaatga
    23521 gtcgttagtt actggctgat agccggtaaa aacgttggga agggctccca tgtcgcaggc
    23581 accctgcaca ttattctgac cgcgcagcgg gttaacaccg ctggcaggtt tacccatgtt
    23641 accggtaacc agtgccaagt tagcaactgc taaaacgtta tctgtacctg taacatgctg
    23701 ggtaataccc atggtgtaga aaatcatggc tgcgtctgct gcgccataga ggcgtgcagc
    23761 tttaacaatg tcctttgctg cgacaccagt tatttcagag gcatactcag gggtatatcg
    23821 ctctactgta actcttaaag cagcaaagtt ttcggttctt tctgcaatga atttctcgtc
    23881 agccaagcct tctttaagga tgacatgagc catggcattt aagagagcaa tgtttgtacc
    23941 aggttttaat tgaaggtgga catccgcgta gtcacatagt tcgatatagc gggggtccgc
    24001 gacaattagt ttagcgccgt ttctagcggc ttgcttaatc cgataaccaa tcacaggatg
    24061 gtttgctgta gtgttagaac ctatcacaaa gatggcattg gcgttttcaa tttcgccaat
    24121 ggtgtttgtc attgcgccgc taccaaatgc agtggccaga ccggccacgg taggagcgtg
    24181 tcacaaacgg gcacagtgat cgacgttgtt tgtgcctaag ctgcgcagca acttctggaa
    24241 gagataattc tcttcattag tcgtacgtgc tgaagacaaa ccagcaatgg catcagcgcc
    24301 gtattgtgcc cccacctcct taaatttagt cgccacaagc tctaaagcct catcccaaga
    24361 agcttctaca aattcaccat ctttttttat cagtggtgtc tttagacgat ctgggctgtc
    24421 aacaaagtcc cagccaaaac gacctttgac acacagatga cctttcatcg gtccctcgaa
    24481 atttggtgta atacttataa ttttatcatt tcgtacatgc atgtagaaag tacaaccact
    24541 accacagtac gggcaagttg ttaacacttt atcaacttcc caaggccgac cttggttcag
    24601 gccttgtttt ggtgtcaagg cacctactgg acagatatcc acgcacatgc cgcaaaagac
    24661 acaagaagat tcttcaatcg ggtcatcata agcgggggtt actttggtgc caaaaccacg
    24721 atacataaaa tcaatagcac ctacaccgtt aatctcatgg catttgcgta cgcatttacc
    24781 acaaagaata catttattaa ggtcccggaa gaaaaatggg ttagtatcat caagcgggat
    24841 ctctctgact tcaccttgat aggggctgcc ttcaacatca tagcggtagc tataatcttg
    24901 tagtttacag ctgccagttt tctcacaggt cagacaatct tggggatggt ctgcccaaat
    24961 gagttctaaa atatttttac gcgccttttt tacgcgcgtt gtttccgtct caaccaccat
    25021 accttcagct acaggagtgg tgcaagaggc tggaaggttt cgcgcaccag ttacttcaac
    25081 gacgcacatg cgacaggcac caactttggt aagctccgga tcatgacaaa aggtagggat
    25141 atcaataccg acagtttgtg ctgcttccaa aacggtcatg ccactgtcta cttgcagtgc
    25201 ctggccgttg atggtgatgt tcaccttact cattttttcc cctccttcac tatttcagga
    25261 taattgcatc aaatttacat ttttccatgc aaacaccgca cttaatgcaa acagattggt
    25321 caatttcgtg gggttgttta cgttcgccag aaatagcatt tacaggacag tttttggcgc
    25381 acactgtgca gccaacacat ttatcttcta taatattata ggagatcaaa ttactgcaaa
    25441 cgcctgcgcg gcatttaccc tcattgatat gctcttcgta ttcatgccgg aagtagcgta
    25501 gtgtacttaa cacagggtta ggtgcagttt gtcctaaacc acaaagcgca ctatctctaa
    25561 tagcagcgcc catctcttca agtgtttcaa tgtcaccagg tttaccttgg cctgcggtaa
    25621 tacgctctaa gatctctaac atgcgctttg taccttcccg gcagggagta catttaccac
    25681 aggattcact ttgcgtaaag gttaagaaga atttagctaa gtccaccata caggtagttt
    25741 catccatcac cactagacca ccggaaccca tcatggcacc agctgcagtg agtgaatcat
    25801 aatcaacaga taggtctaat tgctcttctg gcaaacaacc acctgatggc ccaccaatct
    25861 gtaccgcctt aaatgccctg tcgtcttgaa tcccgccacc aacatcataa attatttccc
    25921 gcatggtaat acccatgggt acttcggcga gacctgtctt tttaatttta ccggttaaag
    25981 caaatacctt tgtgccttta ctgcgttcgg caccaacctt accgtattca tcggcaccaa
    26041 gacggaagat aacgggcaca ttggcgtaag tctccacgtt attcagtaag gtaggtttac
    26101 cccacagccc ggagtttgcc ggaaatggcg gacgtggacg tggtatacca cgcagtcctt
    26161 caattgatgc caagagagct gtttcttcac cgcaaacaaa cgcgccggca ccttccttaa
    26221 ttttcaaatc aaaacaaaaa tcagtgccca ggattttgtt gccaagatag ccatgcgctt
    26281 tagcttcttt gatcgcaatc tgcaggcgtt taatagccag gggatattca gcacgtacat
    26341 agatataacc ttcgtctgca ccaatagcaa aagcagcaat ggtcatacct tcaattagcg
    26401 catgtgggtc accctcgaga acactgcggt ccataaaggc accaggatca ccttcgtctg
    26461 cgttacaaac aacatacttt ttattgcctt tggcttggta agtgaagttc cacttcatac
    26521 ctgtcgggaa cccagcacca ccgcgccccc gtagtcctga cttttgcact tctttgatga
    26581 cttcttcctg agtcatttca ttgataactt tagttaaagc tatatagcca tcaattgcta
    26641 gataactatc aatgctttcg ggatcaatca ctcccacgtg gccaagcaaa acacgatgtt
    26701 gtttagccat aaaatggtca ttatcagtag ctgctgcact ggacagcaca agcatttctt
    26761 caaccggttt accgccaaca atatgctgtt ccacaatggc agctacttta tctgccgtag
    26821 cgtcaccata cagataagtt tcacctgctg gcgtggtgac ttccactagt ggttcactaa
    26881 agcacatgcc aatacaacca gttctttcgg gcacaatgtc gtgttccgct agcagcgaca
    26941 ctaacttatc gtaggttttg cttgcaccag cagcaatgcc gcaggtagcc agaccaactc
    27001 tgactctata agattgggct tccatcgttg caatccccct tctcctcaat gtgtggtggc
    27061 aagcttagta gttattaaga atctctttta ttttttctgc tgtcagacgc ccaaaggtat
    27121 cattattaat catcatcaca ggtgccagac cgcaagcacc aatgcaggcg acagactcta
    27181 aagtaaattt ctggtcatta gatgtttcgc caacgtctag acctagctgt tctttaagtg
    27241 cttcaacaat cttagacgct ccgcgaacat ggcaagctgt cccttggcag acacggataa
    27301 tattttcacc ccgtggtgtc aagtgaaatt gagcataaaa tgtggcaaca ccataaaccc
    27361 ggctaaatgg cacttttaac tgtttagcag tgtacgccat agcctgacga ggtaggtagc
    27421 cataaaattc ctggatagct tgcagaatag gtatagttgt acctttctgt ccttgatagc
    27481 gttcaagaat ctcatctaca ggtgcataat caaaagagtc ttcagcatgg cattcatgag
    27541 cttctttcaa tgtgtctaac ctccttttca cgtggtataa ataatattta tggctcaatt
    27601 caataacatt cgtgctgctc actactgatt cctgcaaaca gcacaaaaaa tccccggctg
    27661 caagtattac tcgtccaggg aaatatatat gctgtagctg tctataattt tactttatct
    27721 ttctatttct tgcaatgacc aatttctgtt ttgcttgcaa agattaacct tttgttaata
    27781 ctttgtctat ctacagtcaa tcactctttt ctccgtaatg ataaaatcta cgcggcgatc
    27841 ccaggggtct acggggatac cattagttat ttgcacctca aaagtaaccg ccactaatgg
    27901 tacaccgtct cgcagctgtg ggaaaaagcg atcatagtaa ccgccgccat aacctagacg
    27961 gttaccttgc aggtcaaagg caacgcccgg tacgaccaca aaatcaatct ctttaggttc
    28021 cagggggcgc agcgcttctg gtttaggttc tgggataccc cataagcctg gctccagatc
    28081 tgcttctaga tcatgtattt ctgataaaat catacgacgt tctttagtaa cagttttggt
    28141 agccactgct cgtttaccat gcgctaatgt ttgcggcacc atcttcatcg tcaagacttc
    28201 cttaccaaag tttaaaaaat acataatcgt ttgcgctgcc tgatactgtg gtaaggcaaa
    28261 caagttctct gcgatcttaa gactattggc tttaacctgg tcccaagtta ataaatctcg
    28321 ttccgctaat attttttttc gtaattcctt tttctcattc atcacataac ctcctgggtt
    28381 aagagtgcaa aggatttctt ttaataggcg cgagcaaaaa taacgagatg tttaccttcg
    28441 ccaccgcagt gcaaacagcg gcctggcgtt tcattttgca agtagaaggg gatgttgcgg
    28501 atggtcgcct tggtttcttc ctttaccgcg gcttcacagt cactgtggcc acaccagctg
    28561 gaaataataa aaccacgttt ttcttcaata atttgcttaa actctgcgta gtcatcagta
    28621 cgatgagtat tttccgctct aaattttttc gctttctcca gcatgttagc ttgaatttct
    28681 gcgagcaggg cgggtacacg taccgtcaag tccgctagcg ccaacgtttc tttgacacca
    28741 gtatcccgac gtaccaacac agcttgtcct gctgccaaat cacgtggccc tacttcaagg
    28801 cgcaggggga cacctttcat ttcccattca ttaaacttcc acccggggga gtattcttcc
    28861 cggtcatcca tttccatacg caggccagcc tgctttagct ctgtaaaaat agctgttgct
    28921 tgttccagca cctcaagacg agctttttta ggcatgatcg gtacgagaat cacttgcgta
    28981 ggagctactt taggcggcat gatcaagccg cgatcatcac catgtaccat aataatggca
    29041 ccgatgagac gcgtcgaaac accccacgag gtttgccaag catgttttaa ctcaccatcg
    29101 ctatccaaaa aggttatatc aaaaaccttg gcaaagtgag tgccgagatt atgagaagta
    29161 ccagcctgta aagccttgcc atcgctcatt aatgcttcta tagaatatgt ccgtaaagca
    29221 ccggcaaact tttctttttc agatttttgc cctgtcacca ccggtactgc taaatcattt
    29281 tctacaaaat cacgataaac ttctaacatc tgcagcgttt cagcttgggc ttctgcttcc
    29341 gtgcgatgtg cggtatggcc ttcctgccat aaaaattcag ttgtgcgcaa aaatggtcgt
    29401 gttacttttt cccaacgcac cacattacac cattggttaa ttaacaccgg cagatcacga
    29461 tacgattgaa tccagcggct atacatttca cagataattg cttctgaagt gggacgcacc
    29521 aataaccgtt ccgttaattt ttcgttgccg ccatgggtta cccaggccac ttccggcgcg
    29581 aaaccttcta catgctctgc ttctttttgt aagaggctct ccggaatgaa aatcgggaaa
    29641 taagcattac gatggccggt agctttaatg cgcccatcta atccctcctg catgttttcc
    29701 cataggttgt agccgtaagg cctaatcacc atacaccctt taaccggcgc ataatccatc
    29761 atttgcgcct ttaaaataac gtccaaatac cattgggaat agtcttgatt ctgtggtgtt
    29821 atttccttga caaattcttt atcttgtttg gccatctttt tactcctcct tctacgctca
    29881 accgaaacta caaaaaattg tttaatgcta ccaaaattat atcatattcg ctaagagggg
    29941 gcaatttacc aataatcaag caaggagcgg ttgcccgctc cttgctgata attattcatg
    30001 tctaaataat tctgtcgaca ggtatcgttc ccctgtatcg ggagcaatgg cgacgacccg
    30061 ttttcctggg cccagcttac gggcaagctg cagggcggca aaaactgctg ctccactgga
    30121 aatgcccacc agcagccctt ctctggccgc caattgtctg gcagtttcca tggcatcctg
    30181 atcgctaaca gttaagactt gatcaataat tgcgctattt aaaactgaag gcacaaagcc
    30241 ggcaccgatg ccctggattt tatgtggccc aggcttctct cctgataaaa cggctgaggc
    30301 tgccggttcc acggcataaa ctttgactgc gggtagtttc tgttttaata cctcacctac
    30361 gcccgtcaca gtaccacccg tacccacacc tgcaacaaaa gcgtctacct gtccctcggt
    30421 ctgctgcaga atctctagtg ccgtcgtggt acggtgtatt tcagggttag cagggttggc
    30481 aaattgctgt ggtacaaaat attccggatg ctcccgcaag attttgtcca cttcggccag
    30541 tgatccttgc atcccttggg ccccgggggt aagaataaaa tctgcaccat aggccagcag
    30601 cagattgcgc cgctccatgg acatcgtatc tggcatcact aaaattaggc ggtaaccacg
    30661 cgctgcagcc agcatcgcca agccaatgcc ggtattgccg ctggtaggtt ccaaaatggt
    30721 atcaccaggt tttaactttc ctgcgttctc tgctgccaga agcatcccta aagccgctcg
    30781 atctttgatg ctgccgctgg gattaaatga ttccagtttg actgtaactt cagccatcgt
    30841 cggctcaact aaattagtaa gcctaaccag cggtgtttgt ccaattaact ctaccacgct
    30901 ttgtgcaatt tgcatcggtt ccctcctgac tggctgaaaa atctgtctag tctcctgatg
    30961 ccccaaaaga tttagtttgc aataattgtg ctgcttttgt caccatctgt ttaggtactt
    31021 tgactactac ttcattcatt acacttgcgg acatactata gccatcaccc aacgaatccc
    31081 tgtcaaggat aacaggaatc ttcgctgctt ccagcatccc tttggccatc gtcgcttctt
    31141 cccatgtggc tgcttcaaaa atagaaaccc agttatcaat accttcgtat ttcataagcc
    31201 gcctccttcc ggatagtagc ttgcccagag ttagttattt ttaagcaaat cttccatggt
    31261 acctgagtcc cgcattttta atagtgtata ctactttttg ccaaacatgc ctgacaaaaa
    31321 tgtttttcct gaagccaagc caaaacctaa aatagaacgg tttttatgga atgtttcatc
    31381 aagagcgttt agcaccacat caatctgttc ttgcgatacc actaaaggtg gctccaaacg
    31441 aatcacgtta ggattattga gagtataggc cgtgataatt ctgtattggt ttaacagcac
    31501 cccggccacc atagaaccca aatattcgct tgccagtttg ttaagtgctc cggcaacttt
    31561 agccattatt cctttagcag gctgctcaaa ttctacgccc accagcaatc ccttaccacg
    31621 cacgtcgcgg atgataggat atttctcctg caaactttgt aagccagtta ataaataagc
    31681 acccatttca gctgcgcgtt cgggaagttt ttcctgatta ataacttcca aagcggcaat
    31741 tcccgccgca gttgcccagg tattaccacc aaaagtagaa gtatgcagca aacacttttc
    31801 cacaccgcca taggccttgt gccaaatctc tgctgtggtg ataaatgcac caatgggcat
    31861 gatgccgccg cctaatgatt ttgccagaca cataacatca ggttctacac cttcatggtc
    31921 acaagcaaac attttcccgg tgcgacctaa cccagtttgg atttcatcag caataaataa
    31981 agtgccatac tggtggcaga gctctttagc agcatttaag taacctgccg gcggtacaat
    32041 tacaccgcct tctccctgaa tgggttcgac aataaaggca gcaacatcgt ttgttttcag
    32101 ctctttttct aaactttcta attcaccaaa aggaaccata ttacaatctg gcagcatcgg
    32161 ttcaaaacct ttatgatatt tttccctgcc ggttaatgaa agtgccccca ttgttttgcc
    32221 gtgaaaacta gcaacacagg aaataatgcg ggaacgtcct gtagcaatcc gagctagttt
    32281 taaggcacct tccactgctt ctgctccgct attacagaaa aaggtattac ttaacttgcc
    32341 gggcgtaatc tgtgctagat tatgggccaa ggcactagct acaccattca tagaagcttg
    32401 cagtaaatta ggcatggctg atactttctg tagggactca atcacatagg ggtgattatg
    32461 ccctaggttt acggaaccat agccaccaag aaaatctaaa taacaattac cgtcgccatc
    32521 ccaaacatta acaccctgtg ctttaacata atgcttgtca aagtcaagca atcccaagta
    32581 ggaacataag ccagggttca tatggttttt aaaattctct ctcacctgcg tacgcgtcag
    32641 ctgaggcaga tctgagattg tatacatttt ttccatgatt atctatctcc tcgccttgtg
    32701 gatacagatt aattccacaa ttactggcaa aatcctgcta tgcagtgttt agtttacgat
    32761 aatgaggccg gactgctgcc cgacctccag acgcacgtca tcaattttta ctcttaccta
    32821 ggcgagcttt ttggccagat cacagagcga gccaggtaat cgaggcttaa gtgttcttgc
    32881 agacgctggt ttacttcatc ttgggtcaga tcatgcagta cctgcagata gtcaaaaaag
    32941 ttaatttttt taaaatgata ggctggatag ttgttagcaa taaactccaa agagttaaag
    33001 cttttaagaa attcacccat cgctttacgt tgatagtggg caaatgtctc tgtcgagata
    33061 ccctcccttt gggcacgctt gatgccctca accaatcgcg caaacaactt ttctggctca
    33121 gctgtttcac caccgatgat ggaatggctg taatcttgtt ctgctacata gccagcatca
    33181 aattcatcat ctactaagcc ttcttcatac agctcgttat aaagtttgga acttgggcca
    33241 aaaacaacat ctaacattaa attcgtagct atctcacgtt tgaaaagctt ttcaccacca
    33301 taacctaaat cttgttcttt aaagccaata tttagcactg gttcagaaac tactaatgtt
    33361 tgctccacat actctttcgc aatttctgct ggctcttgag gaaagagccg cttaatctca
    33421 cccagaggct gataatcatg cctttggata ttctcttcaa tttgctccaa cacccgctcc
    33481 gcgtccacat caccagtcac aaacatagcc atattactgg gatgataaaa agtgcgatag
    33541 cactgataaa gcacctcttt atctattttg ctaatacttt ctaccgtacc ggcaatatcg
    33601 atcctgactg gatgttcttg gtacaaagca cctaagaggt taaaaaagag ccgccactgt
    33661 ggattatctt catacatacg aatttcttga ccaataatgc ctttttcctt ttccacactt
    33721 tcctctgtaa aatagggatc ttgcacaaag tccaataata actctagacc ttcagtaaag
    33781 ttttctgtag cagaaaatag ataggtagta ctggtaaaat tggtgaaagc attactagag
    33841 gcacccaata ctgcaaagcg gtcaaaggcg ttgccctgct catcttcaaa cagcttatgt
    33901 tccagaaaat gtgctacgcc atcaggaacc tgcagctccc gctcctgacc ttcgacaatg
    33961 aaacgtgagt taatagaacc gaagttggtg gagtaggtgg catacttttt gttgtagcct
    34021 tttttaggta aaacaaagac ttccagacct ggctcaatcc gtcgccagga gatcttttcc
    34081 cttaagaggc tatgctcaag tacttttgtc attacacgcc cccctttgta agaaagtaaa
    34141 ctgtgtctaa agctattttt tctgcgatgc gtacaatatc atcacgggtg acggcattta
    34201 aacgtgccaa taaatctttt gtatcaaaga cccgctctgc taaaatacca tctactgtac
    34261 gattaatcag cgctcccggg tggtcttctt ctaccaaata ttgattacgt aagccactgc
    34321 gggtattatc cacatcagct tggctaaatt ctcctttttg catatctgac acctgttgat
    34381 taatgatctc taaagtacgt tcgtaattat ttgtttcaat gccagatgag ataaccatca
    34441 gccctttatg tttttctaaa cgtgaataag tatagtaggc taggctagcc ttttcccgta
    34501 cattttgaaa cagtttagag tgcgggaagg ttcctaggat gccattatac attactaaag
    34561 cggcataatc atcatcgcca taatgcagtc ctgtgcgata acctaaaact aatttgccct
    34621 gactaacatc catttcgtcc cgtatataac taacttcacg tggctgtact gcaacttttg
    34681 ttggcggcac tttcttttct gtgccgcgct ggtaggtaaa aatcttctgc accagggcaa
    34741 atgtctcttc aacatttaga tcgccgatga tatgtaagtc aaccggactt tcctgcagaa
    34801 aggtttgata aaactgaaat agtgatgtcg catcaagttt acccagctgc tccaaactgc
    34861 catatttaaa gacgctgaag ggctcctctg cacacatttc ttgcagacag cgctccacgg
    34921 agtaagctat cttatcatta atcaggccgt tgatgacttt ttcatgctgt tctttttcct
    34981 gctctacata gtccgcctta aaggtatccc ctgctagcag tgggttggtg agcaaatcat
    35041 gcagaatttc caagcctttg cgcagcagtt gatgctcctc gggcaaaaac tgatcgtgca
    35101 ccaaatccat cgtataacag attagctggc gttcaccctt cttgatcact tctgacatca
    35161 gctcagtacc atagagctct tctaaacgta gtgctaattt ttgccgtgtc ggatatgtac
    35221 ccgtaccacg ctgtaaaatt gccggtaaaa gggcgctttg cgtcacctca tcattaagca
    35281 gctgacggtg aaaaaaaagt tgtaccaaaa tcgttttgaa ttttgctgtg ggatgaatgt
    35341 agacattaat gccattttcc aggcgtttgt gcttaaatat agccaagttt tttcctcctt
    35401 cgccaccatt cgcttatctt ccaacaactg ggcatgtaac tttattccct tctcaaaggt
    35461 ataatcctgc cactgcttct attttacgta tcttttagtt aaatatttat tattgggcgt
    35521 ggagtttctc gagccaccct agatgctaaa ataatcagcg gtactcctgc atatagaagc
    35581 gtggttgcag cagcgacaac tccggagtca ttcaccagca aagctgttac acttcccgca
    35641 ataattccta aaaaaccagc cgctaaatca ggatacattt tatgaatatc cactaaaatt
    35701 ccctgcggac ggtaaaacaa tacggccagt acccctaaaa aagctaagag aacccgactc
    35761 cactgtgaat agcgaaagag ctttagattc atggaccctt tacgaatgat gatatccttt
    35821 aatgcctgcc aaccctgctg ctgcagcaag acaagcgttc gccctaaatg cgattgtaaa
    35881 ttttgtggca cgtagatgtt aattaaaata gcaaggatgg ccacggccac taagctgaca
    35941 acagtaatac tggcactttt ccaactgaag cgaaaattaa acaaaactgc taaggtagta
    36001 ataaaagccg caatagctgt taaagtacca ccaaaattag caccaccgtt gggtgctacc
    36061 atcagcagcg caatcccagc aaaaagtatg gtacttaagc ctagtaacca tttacgccaa
    36121 cgcggtgcta gtgtcgtaag agctgtgacc cccagtactg ttgtgcccac ccaaataccc
    36181 atatattcat tgcctaagcc ataataacgt gcaccggaaa ctggatcata acccagcact
    36241 gaaatctgca ttagctgtgc atcacgaaaa atatctatca gtaaggagag tgatgtggca
    36301 caggcgatca aagcaaagcg ttctatcggt cccgctaata gtttctgtaa aagcagcacg
    36361 acaattacaa cggctgcagt acaaacaata aaactcaggc ttaacgataa ctgcgccaac
    36421 ggtagataca gcagcagcag gggaaagctt aacagcccca gcagggcact ttcaaagtat
    36481 ttagcaggta tcaggcgtaa aaagaggttt aaaagtgcgc ctagcacaaa aataatctga
    36541 cagatcacat aaaccctcaa gaggggtgaa cgtaagcgat aaatacggtc aatggtactt
    36601 tgcattgcca acaagtcttg cacattgccg ctgcccgcca actgcagagg acgcccaatc
    36661 atggcttgcg gcagcgccaa acccagttgt gtgagcacta gcggcgcaag atcggttacc
    36721 gcagccaaac ctgcgcgctg cgttgtagca gaggttacta aacgtcccgg cagaaaagaa
    36781 ccgcccctaa cagcaacaag ccccaaggtc tcagcagctg cagcaccatt agggggcacc
    36841 gctgccagca aaagcagaag atcatctctg tgcaggaccg cagttaaacg ttccagcagc
    36901 caatcaatat ccctaaatat ttcttcttcc agctgctttg ccacctgctg acgcagttgt
    36961 gtgcgataca tatctaaccg agcagtatcg ccccaatcaa tgagtatgac atcagttttt
    37021 gcatatagct cccgaaagat ctgccaaacc tgctctttat gcaaacgcaa cccataagga
    37081 aacgctgcat catctaataa aattgctgtc ccaacctggc caaagtcaat ttcacctttg
    37141 ctatcggcta ggatggctgc cgcttcacga ttaacttggc tggcatcagc attaccgatg
    37201 acggctgtct ttttaccgcc tgcatgcaaa gcatcaccta ataaccctgg ttggacaggg
    37261 taatttagcg tttggttggc atatattaaa tcggccatcc ctacatgcac aattcttcca
    37321 gaagcagtag aaccttggtg gcgcgtaaaa attatttggg caggagtacc gttatactct
    37381 tcttcctgta aaaatgctaa acgggtacta tcattaccta aagcgcgtga acctgcgccc
    37441 atggtaacat aaagatgacg actgacagca ttgctggcag tacctgtatt tagcagccct
    37501 accgcactct gtgccagcca ggtggccatg ttttcatcgt cagctaattg ctgtgtactg
    37561 gtgcgactta aagaaacaat gaccacttta cccgccgctg cggcctgtgc ggggttagtt
    37621 aataacagta agcagaagaa actgaataat aatgcctttt tcaaagcgta ttcctaccct
    37681 ccaaaaccct atcataaacc attttcgttt tcctcatact ttcgctgatg ctaaaacggc
    37741 acacatccgc ttgcgcctgc ctcgccagac gagctgccaa gtgcggctgc cgcagtaact
    37801 tttcaatagc ttctgctaaa ctgacagcat taccgggtgg tactaacagc ccgtttttat
    37861 tatcctcgat tagctccctc agcccccccg cagcactaac cacgacgggg cagccagcct
    37921 gcatggcctc cagtactgcg ataccctgcg cctcactaag agaaggcagc acaaagaccg
    37981 acaatcttgc cagcagcgcc gccacctgct cacagtaacc aagaaaaaag aagcgctgtt
    38041 ccaaagccaa attatttcgc tttttttcaa gctcggcaca taacggtccc tcccccgcca
    38101 ccacaaaaca aatttgtgga aaacgaggca ccagcagcgc tgcagcctgt aataatactt
    38161 caagtccctt ttgcggtact aatctggcca cagtacctac taaaatggct tctttactaa
    38221 acggtaaagt acaagctgcg gctttatcag ccatcaactc aatgccgtta ggcactactg
    38281 aaatctgccc ttcatcaacc tgaatatttt gctgcaatat ctcggctaac cactgcgaaa
    38341 cagtaatata acccgccgtc cagcgcttaa gttttcgttc cgctgttaga tagatagcag
    38401 gcaggcgggc tgcttgcgcc tgtgcaaagt cactgtgcac agtaactaaa caaggacgct
    38461 tacaagcgcg cgccgccagt ctggccacca gagcagcttt aaagccatgg gcatggagca
    38521 gatgtaccga atttgtgttg ataaagcgca ccagctgccg catcgcctgc agatcagaca
    38581 acggcctcat ttttcctgat aagggcaggg gtaagacttg acagggcaag cctgcgtaat
    38641 ctgcctgctg ctcttgggga caagcgaggg taaaagtaaa atcagcagct aaatatgtaa
    38701 ttaatgagcg cagatgctct ctaataccac ctacagccgg tcgtaccacc aaaaggatgt
    38761 gaactttctc catgacatta ttccatgcct tttttcgtat agtataacca agtttaacta
    38821 ataaaccctc tcaccgccta ctggcagtac ttaccagata attacacctt gacagtccaa
    38881 aagtaaggct aggtgatgct aatcatcgtg ttggtgttgc tctcttgccg gtaatgcccc
    38941 agtttgacac tatcggcagt actgttagtg taaacttact atagggcttt tggaggaaaa
    39001 cacccccaat ttgtagaaa
//
LOCUS       DASRMS010000187         3152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_591070, whole genome shotgun sequence.
ACCESSION   DASRMS010000187 DASRMS010000000
VERSION     DASRMS010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591070"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..203
                     /locus_tag="VFC74_08855"
     CDS             <1..203
                     /locus_tag="VFC74_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25484.1"
                     /translation="LDLLERAKSEIKATRYTISWRVGPEILTLCQQTNDDGLINAYRK
                     HFMKFNAKAAHAAAIWHEQHRN"
     gene            complement(800..2482)
                     /locus_tag="VFC74_08860"
     CDS             complement(800..2482)
                     /locus_tag="VFC74_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25485.1"
                     /translation="MIGPTTYTSLAGSRLASSSTTQAGKTPEASATDKAGTAASTSNA
                     GATSSVSSWARQLGEAAARAEIRDSSLSRKELGEKAAAQLSQITGSQYFQQQAQHNAQ
                     VPATDDPELLARAQQATSFVNGSGTNPFKGLSADQLALIAYDDGDTFTVNERRAAWLE
                     SDSQRQGWQQQAVAMGMLEHNLTDKMNDFHTEALIHYNLQPAIEQARYPENYANRLQA
                     QISADDHPQHQGKEAVNSAAKTVEQLFAATPHANDIEKQVTTVSVQAVAATQAAEELA
                     YTNPRSTPAYKLMVDRLFGGKEPPVGNGAEGMSGSNIGRSSYNFLTYPDRILLSDVYA
                     YAQTEGADLAYVDRLAAKLGDYRQHNNGQLSSSFNNGHSYDAEGRQLTVSFNEKDTAT
                     ASRILNGSAINSTAFDRGFLNHILDPGYGAIGHFTNFEFLEHMVNKFSNEGIEVAPID
                     NKFATFKPVGVIKDHIVLTANENVRLKPSEPDIVKVNGVYTLTEKGRAAGITMDEVHG
                     RTSKVSSASVSREMNRYILDAFFNKKDSTKTHTSLIDLLNQQEVNFKTKRNN"
BASE COUNT          803 a          784 c          720 g          845 t
ORIGIN      
        1 cgcttgattt gctcgaaagg gccaagagcg agatcaaagc tacgcgctac acgatcagtt
       61 ggcgcgttgg cccggaaatt cttactcttt gccagcaaac gaacgacgat ggccttatca
      121 acgcctaccg aaagcatttc atgaagttca acgcaaaggc agcccatgcg gccgcaattt
      181 ggcatgagca gcataggaac tagagtacgc ctgtcacgta taccctaagt cgtgcgacag
      241 tatattctcc tactgcttgc ctagcgttgt cgtgtcgcgg gttctagttg catataatga
      301 acacacgact agaacagaag acaatacaca atgaataaaa acgccactgc tgggctatgc
      361 tcacgttagc atcgtcaacc aagacttgac caaccagcgg gttgagctgc atgctgctgg
      421 ctgtataagc tattttcata gaaggtcacc ggcaaccggc gcaaccgccc ggagtatgca
      481 tacttgcgcc tcgacgatgt gctaacagtg ctccaccttg acgggctggc ccgcagcacc
      541 cgcaccaagg cggcaaaagt tcgttatgaa ttttgtaacc agcggtcaaa acaggtcaat
      601 aaacatctca cgcctgtgcc ttagagctgc gagcatgcgt gctgacacgc ttggggtatt
      661 aacatagtta acaacaccga atgagcaaaa tctacttaag aatttagtca aacacttcta
      721 acggaatcta ttgcaatgct aaagcaacat aggcaattgt taaatttttt gactgaaaac
      781 tacctacctg ccacgagccc tagttatttc ttttcgtttt aaaattgacc tcttgctgat
      841 tcagcaagtc aattaaggat gtgtgggttt tggtcgaatc ttttttgtta aaaaaagcat
      901 ccagaatata gcggttcatt tctcgactga ccgaagcgct tgacacttta ctggtcctgc
      961 catgcacctc atccatagtg atgcctgcgg cacgaccttt ctcagtgaga gtgtacacgc
     1021 cattcacttt gacaatatca ggctcagatg gctttaagcg cacattttcg ttggcagtta
     1081 aaacaatgtg atccttaatg acacctacag gtttaaaagt ggcaaattta ttatcaatcg
     1141 gcgctacctc tataccttca ttggaaaact tgttgaccat atgctcaaga aattcaaaat
     1201 tagtaaaatg tcctatggct ccataacctg ggtcgagtat gtgatttaaa aaccctcggt
     1261 caaaggcagt actattgatt gcgctgccat taagaatacg cgaagcagta gcagtatctt
     1321 tttcattaaa gctgaccgta agctggcgtc cttctgcatc atagctatga ccattattga
     1381 aactcgagct gagttggcca ttgttatgct gtcgataatc acctagtttt gcagccagac
     1441 gatctacata ggctaagtcc gcaccctcag tctgagcgta ggcatacaca tctgacaaca
     1501 aaatacgatc tggataagta aggaaattgt acgagcttcg accaatatta ctgccactca
     1561 tgccctccgc accgttgcct accggtggct ccttaccacc gaacaggcgg tcaaccatca
     1621 gcttgtatgc tggtgtagac cttgggttgg tgtaagcgag ttcttctgcg gcctgtgttg
     1681 cggcaactgc ctgcacgctg actgtagtga cctgcttttc tatatcattc gcatgtggcg
     1741 tagcggcaaa gagctgctca accgtcttgg cagcactgtt aacagcttcc ttaccctgat
     1801 gctggggatg gtcgtcagcg ctgatctgcg cttgcaggcg attagcatag ttttccggat
     1861 aacgcgcctg ttcaatagca ggctgcaagt tatagtggat aagcgcttct gtgtgaaaat
     1921 cgttcatctt gtcggtcagg ttgtgttcca acatgcccat cgctacagcc tgttgctgcc
     1981 atccttgccg ctgactatca gactccagcc atgcagcacg ccgctcgttg accgtaaagg
     2041 tgtcgccatc atcataggca atcaaagcca gctgatctgc tgacaaccct ttgaacgggt
     2101 tagtgcctga gccattcacg aaactggttg cctgctgtgc ccgagccagc aattccggat
     2161 cgtccgttgc aggcacctgc gcattgtgtt gcgcctgttg ctgaaaatac tggctaccag
     2221 tgatttgact gagctgtgca gctgccttct cacctaactc tttacgactg aggctgctgt
     2281 cgcgaatctc ggcacgagcg gctgcttcac ccaactgacg tgcccaactg gaaacgctgc
     2341 tggtcgcgcc cgcattgcta gtggacgcag cagtgcctgc tttgtcagta gcagatgcct
     2401 ctggtgtttt tccggcttga gtagtggagc tgcttgccaa gcggctacct gctaaggatg
     2461 tgtaggtggt tgggcctatc atgagcgctt ctccatatca ctgcgacaca aagcgttgtg
     2521 caagtttata tctgacaatg caatcatcgg cagcccagca aaatacttta gacttccata
     2581 ttgtttctgg ttaatgctgc ttcatgacaa acgaatataa ataggcatcg caagcattta
     2641 tgaatgcctg caacacctat gttaagtatc agtagctgta gtggatagta actgtatcct
     2701 tgcctgcagg gtagcctggc tgctgcccac cctgtactcg gtgccgaatt atcaccttag
     2761 ctccgggccc gaagttctga ctattaaagt cagtcacagt gccggtacta ttaggtgaaa
     2821 ttgacttaca acccacagga gtcacgctac tataaggacg gtaataacaa agctcgacag
     2881 tttcatcaat attatttgga tagttggtcg tactgtagtc gatttttttc aaggatttag
     2941 tcttagtccc tgcgccgctg ggaaaatcag tgctactcaa accaaattga gctaaattac
     3001 aggcattaaa cccagttcac cattcgacta tactgtaaac ttcgagatta aataatttcg
     3061 attcgtttgg ggctggtctg actagccgcc tcaagcactg gaggttgaaa tgaacattgc
     3121 aatcaattcg taccagatta attattctgc ca
//
LOCUS       DASRMS010000188        39146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_322155, whole genome shotgun sequence.
ACCESSION   DASRMS010000188 DASRMS010000000
VERSION     DASRMS010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 39146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39146
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_322155"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..761)
                     /locus_tag="VFC74_08865"
     CDS             complement(<1..761)
                     /locus_tag="VFC74_08865"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HZK25486.1"
                     /translation="MPWGTLVGVRPAKQLHKLIDQKLDYQAAFSRMHAATKISKEKFF
                     LLWQVCQKERAILEESTNPQLFSVYVGIPFCPTRCLYCSFPSHSLTELGSLRSQFTTS
                     LLQEIAATGALTQAFGMHTYTVYLGGGTPTALNPRELEKIILALRQAFPGSWREFTIE
                     AGRPETLTAEHLEVMIKHGINRISVNPQTMHQITLDRIGRCHSPADIVEAVEAVRKAG
                     ISILNMDLIIGLPGEEQKMVKESVQQVLDLHPENIT"
     gene            complement(849..1538)
                     /locus_tag="VFC74_08870"
     CDS             complement(849..1538)
                     /locus_tag="VFC74_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25487.1"
                     /translation="MSYLVSKNMIRANTTFRGLPVPAAIALTCPHCHDYLTYFCRDYV
                     HHAATNTITFLGICPACAAKVSFWLVNCRDYENSQIFNYPSFNQSREMMPGIENVPQP
                     ITDNYLEALETYHHQYYKATTTMVRASLEDIFKTNLGQGEVKELAALIRQLAEKVNLT
                     EQVNSVAATIGNNQQLLPYFAFEKKPTQQTAAVLLKLLEFVLIQTYVIPAATQRLQHE
                     VDCLAQEKGTE"
     gene            complement(1671..1746)
                     /locus_tag="VFC74_08875"
     tRNA            complement(1671..1746)
                     /locus_tag="VFC74_08875"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1711..1713),aa:Lys,seq:ctt)
     gene            complement(1784..1859)
                     /locus_tag="VFC74_08880"
     tRNA            complement(1784..1859)
                     /locus_tag="VFC74_08880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1824..1826),aa:Gln,seq:ctg)
     gene            complement(1864..1949)
                     /locus_tag="VFC74_08885"
     tRNA            complement(1864..1949)
                     /locus_tag="VFC74_08885"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1912..1914),aa:Tyr,seq:gta)
     gene            complement(2003..2089)
                     /locus_tag="VFC74_08890"
     tRNA            complement(2003..2089)
                     /locus_tag="VFC74_08890"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2054..2056),aa:Leu,seq:cag)
     gene            complement(2098..2173)
                     /locus_tag="VFC74_08895"
     tRNA            complement(2098..2173)
                     /locus_tag="VFC74_08895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2138..2140),aa:Lys,seq:ttt)
     gene            complement(2215..2290)
                     /locus_tag="VFC74_08900"
     tRNA            complement(2215..2290)
                     /locus_tag="VFC74_08900"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2255..2257),aa:Gln,seq:ttg)
     gene            complement(2295..2380)
                     /locus_tag="VFC74_08905"
     tRNA            complement(2295..2380)
                     /locus_tag="VFC74_08905"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2343..2345),aa:Tyr,seq:gta)
     gene            complement(2396..2471)
                     /locus_tag="VFC74_08910"
     tRNA            complement(2396..2471)
                     /locus_tag="VFC74_08910"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2436..2438),aa:Thr,seq:tgt)
     gene            2742..3737
                     /locus_tag="VFC74_08915"
     CDS             2742..3737
                     /locus_tag="VFC74_08915"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HZK25488.1"
                     /translation="MNDNEFIKIFNDLERVEKELRKVGNNQEQRQKCYDRLLELRRRM
                     DHYVEFWLRFEEKINEMQEEYDFLLPDEMPESFLQAFGTILPEADAHEMQTELEEVKT
                     DLDRRGVKPLLLQGDNEACIRSFRRGLGFLELAMMDEAIQEFREVIHQEPDLLLAHVC
                     LGVAYAERGLADEAMRELRLVQALTDDQQTRVVVHNALGNVYADKEDYEQAKIEFAKV
                     IELDPDFTIAHFNLGAIYYNLKMYEESIQEFQFVKNKFPKDWEIYFYLGLSYCKVDNA
                     EEALVNLLKASYLVPHNPYVAFELGLLYDNLGETRRALECYYRSRKLYQEQEVQL"
     gene            3821..5065
                     /locus_tag="VFC74_08920"
     CDS             3821..5065
                     /locus_tag="VFC74_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HZK25489.1"
                     /translation="MSTIRDANLAPSGKLKINWVREHMPVLNELAQGFRQTKPFTGLR
                     IAICLHLEAKTAYMAKVLQESGAEVTITGSNPLSTQDDIAAALVADGLTVHAWHGATA
                     AEYDDHLHKTLQSEPHLIIDDGGDLVTMLHTQYPQLLPQVRGGAEETTTGVIRLRSMA
                     AEGALQIPMMAVNDANCKYLFDNRYGTGQSVWDGIMRATNLVVAGKTVVVAGYGWCGK
                     GVTMRARGLGARVIVCEVDPIKAVEAVMDGNTVMPMIEAAAHGDIFITVTGCRDVITH
                     EHFVRMKSGAILCNAGHFDVEVNKGDLRELAGEAQLARHNVEAFEFGETRLYLLAEGR
                     LVNLAAADGHPAEIMDMSFALQVEALHYLNVNYGQLPNEVLPLPQSIDDKVARHKLAA
                     LGMRIDQLTSAQENYLRSWKQE"
     gene            5076..5864
                     /gene="mtnP"
                     /locus_tag="VFC74_08925"
     CDS             5076..5864
                     /gene="mtnP"
                     /locus_tag="VFC74_08925"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015756785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HZK25490.1"
                     /translation="MTIHLAIIGGTGVYDPKLLGAVERLEVTTRYGTALLTQGIYQGE
                     KVVFLARHGIEHGTPPHKINYRANMAALVKLGVTRVIATAAVGTLNETMPPGSMVLLE
                     QFLDFTKGREATFFDGGEAGVVHLDFTQPYCPQINQSLQQAAQAAGLELLQGGVYVCT
                     EGPRFETPAEIKMFKLLGGDLVGMTNVPEVVLAREAGLCYNTVALCTNFAAGLAPQEL
                     THQEVLDVMAENVDKVRRLLAEVIPRLGTERSCRCQSLGHEILL"
     gene            5874..6914
                     /gene="mtnA"
                     /locus_tag="VFC74_08930"
     CDS             5874..6914
                     /gene="mtnA"
                     /locus_tag="VFC74_08930"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HZK25491.1"
                     /translation="MKPLGIKNQQLLLLDQRLLPTQVEYLTCENWQSVAQAITAMVVR
                     GAPAIGAAAAYGLAMAATSLQAAAPAQFAAGLQEAAAGLQAARPTAVNLAWAVKRMLN
                     VARASTRLDAKDIAARLWQEADKIATEDVAVNKKIGEYGAALIPAQAHILTHCNAGAL
                     ATVGYGTALGVIRSAVAQGKMIAVYADETRPYLQGARLTAWEMVQEQIPVTVIADSMA
                     GFLMKQGKVDLIIVGADRIAANGDAANKIGTYTLAVLAKAHKIPFYVAAPASTFDLSL
                     TDGEQIPIEERDERELIFFAGTRIAPEGVLCYNPAFDITPAELITAIITEYGVIERPT
                     AAAIAAVLGEKI"
     gene            6911..7567
                     /locus_tag="VFC74_08935"
     CDS             6911..7567
                     /locus_tag="VFC74_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014827247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HZK25492.1"
                     /translation="MKDCQTEILQTVHSLCQEHLVLGTWGNVSTREAQYFWITPSGMP
                     YDCLKHDDLIKIDLATGQAAGPWKASTEWPLHAAIYRGRPDCAAVVHTHSVYATAFAV
                     ANLPIPPVVEDLVQIVGGGVAVADYALPGSKELAQQALLALADNNAVLLANHGLVGIG
                     KSLTEALTVCRIVEKTAQVVIQAKALGPIREISQSDIKVMRQFYVTAYGPGHKREERP
                     "
     gene            7564..8868
                     /locus_tag="VFC74_08940"
     CDS             7564..8868
                     /locus_tag="VFC74_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HZK25493.1"
                     /translation="MRTLIQKTTVITSHTQPEILEDADVVIDGQIFSYVGPSQAWQED
                     FSQIIDGRDKVVAPGFVNAHSHAAMSLLRSYADDVPLMYWLEKRIWPIEARLKREDVY
                     WGTKLALAEMIKGGTTAFADMYFFMEQVAEAVEEAGMRAVLCRGLVGVGQEAESGLEE
                     SKHFVQRWHGRADGRITTMLGPHAPYTCPPDYLKRVLALQEELQVPIHIHLAETTDEV
                     EDIRKQYQLTPVQLLKDVGLLDRPVLAAHCVHLTEDDMDILKAYDVHVAHNPGSNLKL
                     GSGVAPVPDLLSRGITVGLGTDGAASNNNLDMMEEMRLAALLHKGVRMDPTVITAAEA
                     LMLATSESAKAIFLDQVGKITEGMKADLILVDLHQPHLTPRHNIIAHLVYAAQPSDIS
                     IVMVDGKVLCENGRLTTLDEDEIIYQAEQRAQFLVKEDQENN"
     gene            complement(8903..9484)
                     /locus_tag="VFC74_08945"
     CDS             complement(8903..9484)
                     /locus_tag="VFC74_08945"
                     /inference="COORDINATES: protein motif:HMM:NF021120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcN/YlaJ family sporulation lipoprotein"
                     /protein_id="HZK25494.1"
                     /translation="MKTKKLVYFLSLLLVASLFVSSCSLFRRPEPERQPVPTPVPDPN
                     LAPNQTPQPNPGVNPPPAPQPQTPREQPPGQPTPDATQMAERIADIATGVQGVDNSVV
                     VVISNMALVGITLERAETAQRGEKELKQEVARRIEDREPSIVNAYVSANPDLVKQLQD
                     ISRGIRNGKPISTFFDQITDVLSRMRAESGNNK"
     gene            9627..10403
                     /gene="surE"
                     /locus_tag="VFC74_08950"
     CDS             9627..10403
                     /gene="surE"
                     /locus_tag="VFC74_08950"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HZK25495.1"
                     /translation="MRVLVTNDDGVYAEGIQALAQEITKIAETYVVAPDHEQSATGHA
                     ITMHRPLLAEEVKYHHLPELSPWKVNGTPADCVKLAVEALLPHKPDLVISGINRGANL
                     GTDVLYSGTVSAAIEGVVLGIPAIAVSLAEYHDPHYEYAAAFTARLAKQFMQQKQEGE
                     MLLNVNVPGCAAEEIRGVAVNRLGVRHYKNPFEKRTDPRGRSYYWLAGEIVDLEGEND
                     TDVAAIKAGKISITPIQHDLTNFLLLDKVREWLTDMKGRN"
     gene            10556..10741
                     /gene="dmpI"
                     /locus_tag="VFC74_08955"
     CDS             10556..10741
                     /gene="dmpI"
                     /locus_tag="VFC74_08955"
                     /inference="COORDINATES: protein motif:HMM:NF041920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0006725 - cellular aromatic compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase DmpI"
                     /protein_id="HZK25496.1"
                     /translation="MPTFFFYGPSLELEKKRTMIKEITAAASKATGIPEKSFTIYLRE
                     TQHENVSVGGKLLSERD"
     gene            10835..11584
                     /locus_tag="VFC74_08960"
     CDS             10835..11584
                     /locus_tag="VFC74_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25497.1"
                     /translation="MRLAPVENKPEKQHIFLQPYFIITFVVVVLSSVCVFLLTRQKEE
                     AVPTMLDIVAETTAAAAAHKHYNLVIEESGPGYLLRFSGQVHDDRLYGKIDGYDLEIF
                     ADEDQFFIKNSLASEWQDIKTAKLETLPTLIRDPHYLLANLMAGKEIAIETGTPRTVD
                     DVICQTYFLEIPPPNLQLLTRFSTDATLDKLQVYLWFAEESGFMHRLAVMMNVTVAEE
                     TIQINRTYNLSPEAKDFPEDIPKINRKVMPI"
     gene            11672..11962
                     /locus_tag="VFC74_08965"
     CDS             11672..11962
                     /locus_tag="VFC74_08965"
                     /inference="COORDINATES: protein motif:HMM:NF039183.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5658 family protein"
                     /protein_id="HZK25498.1"
                     /translation="MAFIIWLLGLADVYLTKYGLQIGVITEGNPIMGYLFNLNSHWAI
                     IFSLSLSGLLLYCLQRLCLRTTMAEKAMKGLLVVRILVIGLHLNWLYQYYCL"
     gene            12020..12214
                     /locus_tag="VFC74_08970"
     CDS             12020..12214
                     /locus_tag="VFC74_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25499.1"
                     /translation="MFYYVCCPECNTDLSQHVDEEDPTESIVICSHCKTALQLQHVDQ
                     WDEDMGFDVTLFWFEKVAEA"
     gene            complement(12270..12344)
                     /locus_tag="VFC74_08975"
     tRNA            complement(12270..12344)
                     /locus_tag="VFC74_08975"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12310..12312),aa:Trp,seq:cca)
     gene            12535..13335
                     /gene="proC"
                     /locus_tag="VFC74_08980"
     CDS             12535..13335
                     /gene="proC"
                     /locus_tag="VFC74_08980"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HZK25500.1"
                     /translation="MREHIGLIGIGAMGSALLKGLLSSGIEPDKIIVADAAEAKLQQA
                     AVTYQVLIGEVCEVATAARVLFLAVKPQDMPSLLLQLSPLLQQDQVVVSVAAGVTIQA
                     IEENLSAQVGVIRVMPNTPCLINAGVLAISAGHHVQEADIHLVETWLKNMGSVYLVPE
                     KLLDAVTAVSGSGPAYVYLFLEALADGGVLAGLPRVLAQELAVETVLGAAQMVKATAQ
                     HPALLREMVTSPGGTTAAALLALEQGGLRGTLQQAVKAAAERSKELGS"
     gene            13336..14463
                     /gene="proB"
                     /locus_tag="VFC74_08985"
     CDS             13336..14463
                     /gene="proB"
                     /locus_tag="VFC74_08985"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007064678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HZK25501.1"
                     /translation="MANNRGLLKNTQTIVVKVGSRTLTHATGKLNLRMLEKIVRELAD
                     LANQGRQVILVTSGAVAAGIGRLGLSAKPQSMPEKQAVAAIGQGLLIQMYEKLFAEYG
                     LIAAQVLLTRDDVNDRQRYLNSRNTLQALLRYGAIPIINENDTVAVEEIEFGDNDTLS
                     AMVASIIAADLLVLLSDIDGLYTANPKVDASAQRVSYVEEVTAKIKALAGETNETLAT
                     GGMITKLTAAEIAGNSGVQMVIANGREQNILQRILDGEDVGTLFCARDKMLESRKRWI
                     AYGQQPQGSLLVDAGAKKALLADGKSLLPSGIVKVNGTFSQGELVAIKDLAGKEFARG
                     LVNYCSAELQTIQGLRTEQAAEVLQKDCGEAVHRNNMVVNS"
     gene            14494..15741
                     /locus_tag="VFC74_08990"
     CDS             14494..15741
                     /locus_tag="VFC74_08990"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010293453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HZK25502.1"
                     /translation="MSEVVRKAAEAKAAAVHLAMASTVKKNEALLAIAAALTAQQAEV
                     LAANTKDLEAFHQQPGYTTALHDRLALNEERIAGMVEGLQQLAALPDPIGEVTGMSVR
                     PNGLQVGQVRVPLGVVGIIYEARPNVTVDAASLALKAGNAVVLRGGSEAINSNKALVK
                     ILRQAVESVGLPAAVITIIEDTDRAAVHELMRLNENLDVLIPRGGAALIKTVVENSSV
                     PVIATGTGICHTFVDATADVAMATAIAINAKTTRPAVCNALETLLVHQRVADQFLPHM
                     LPQFFAAGVEVRGCEITQQYDQRVAAAKEDDWSTEYLDLIISIKVVASLDEAINHIKR
                     YGTGHSEAIITALYDNAREFQARVDAAAVYVNASTRFTDGFEFGLGAEMGISTQKLHA
                     RGPMGLTALTSTKFIINGNGQIR"
     gene            15889..17055
                     /gene="nifV"
                     /locus_tag="VFC74_08995"
     CDS             15889..17055
                     /gene="nifV"
                     /locus_tag="VFC74_08995"
                     /EC_number="2.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004410 - homocitrate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homocitrate synthase"
                     /protein_id="HZK25503.1"
                     /translation="MKRFLEGLANNQVMLVDTTLRDGEQTAGVVFCNKEKVRIAQMLD
                     TLGVHQLEVGIPAMGGAEKEAIKSIVDLGLDASIMAWNRADVNDVMNSLDCGTDALAI
                     SISTSDIHIKYKLQKTREWVLDSMAKAVTFAKKHDKYVSVNAEDASRSEMEFLLQFAK
                     RAKECGADRLRFCDTVGILEPFKTYELVEELRTTSGMAIEMHTHNDFGMATANSLAGV
                     KAGATMVGVTVNGLGERAGNAVLAEVAMALKYLYGIDVKVNPRDLRELSEYVALASGR
                     ELPVNIPIVGTNMFAHESGIHADGVIKNPATYEVFQPEEVGLQRQIVIGKHSGSAAIV
                     AKFKEYGITLAHKEASELLPQVRSAAIDLKRTLFDKELMYIYEEYKDKNKCKGA"
     gene            17134..18579
                     /locus_tag="VFC74_09000"
     CDS             17134..18579
                     /locus_tag="VFC74_09000"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HZK25504.1"
                     /translation="MKLSQLFAHYPLPSASQIDVEVQGITHDSRYVKPGDIYVCLEGL
                     RVDGHLFAGQAVQKGAVAVVATKPLYLPVPVVYVSDTRHALSFLADKYFDHPSRKLHV
                     VGVSGTNGKTTTTHFLQAIYKAAARPCAVIGTVGIRIDKEYLSGGLTTPESFDLHRTF
                     QEMLERQLRYVAMEVSSHSLTWQRVEHVEFNTVIFTNLSHDHLDFHKNMEDYFQAKAH
                     LFALLKQEESGRPQAIINADDLYGKRLLTMADVAVLSYGLQDGADVRGFITSTSVGST
                     LVLVRYQNLEFELNVHLPGEFNVYNALAAVAAALAEGIAPEQISEGIDNLEAVPGRLE
                     AVNYQQEFNIFIDFAHTPDGLEKVLQTLSEVPHRKLITLFGCPGDRDRTKRPVMGRIA
                     EVYSDVVVVTSDNPASESPEEIIREILAGMEHLPVVLPDRQEAVRYALSIAGKGDIVL
                     LAGKGHETYQLIGDQHIPYSDKQAVETFFIQ"
     gene            complement(18647..19705)
                     /locus_tag="VFC74_09005"
     CDS             complement(18647..19705)
                     /locus_tag="VFC74_09005"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HZK25505.1"
                     /translation="MQTRIVDHDGAELYDEFLAKSAYGDMLQTFSWGEIKKTDWEAVR
                     AVVEDDNQQICAAMSILLRRIPLINRTIAYVPRGPILADTTDSQLIHYTLEALTTLAR
                     QHGAIVIKIDPAIPEGLLSPQDLQDNGFHLTGTDHEFGGTQPRYTFRLPLTGSEDEIF
                     ARFTKKMRYKIRYGPKNGLSFRSNEETSIADFYTALSQTGERNDIMTRSPEYFQRVYD
                     ELKKDDRVLLCTGYIEGEPVISSLTFALGDKAWAVYGGQTNKYRKLYTYHAMNWERIK
                     WAHRKGAVWFDFYGVPGNVEEDHPLFGLYQFKQSFGGDYLAFIGEWDKPLSPAFYWLW
                     QTGLPRYRSLLHRLFRHQ"
     gene            complement(19782..20003)
                     /locus_tag="VFC74_09010"
     CDS             complement(19782..20003)
                     /locus_tag="VFC74_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25506.1"
                     /translation="MLGHICPYCNGLEELHFTCPACGGKMADYGALQNALGPYAPYEE
                     NSQLRGQDGCLHQLYCPECELQLQMIIPL"
     gene            complement(20132..21268)
                     /locus_tag="VFC74_09015"
     CDS             complement(20132..21268)
                     /locus_tag="VFC74_09015"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HZK25507.1"
                     /translation="MEVINIPAQAALKYAQLWDGFSQNLHNSGTLLINGGVIAEVNSS
                     SHDNYPVWDFSQATILPALIDAHIHLAFPEEDTTPFTTRAQQYLAAGVAAVRDAGSTL
                     SVSTTNTPLLHLQSINAIYQQGSYGSSLGIATANLREALQAVDKLAAAGAAQIKVIAS
                     GIFSFSTYGKTGAATFSAAELTEIVNQAASYHLPVMAHASGDEAVRRCLQAGVTTIEH
                     GYFMSRATLQELAASDTCWIPTLSPVNAQLTKPELFTALTPSMHTVILRSLLRHQELI
                     AEGAALGAKIAAGTDAGAPGVLHGTALPEEILLLHNAGLTNFNALQAATSLAAQACQL
                     TQLGSITPGKNPYLLVLSGNPLRDLNVLRTPLALILPAARAWQQ"
     gene            21537..22091
                     /locus_tag="VFC74_09020"
     CDS             21537..22091
                     /locus_tag="VFC74_09020"
                     /inference="COORDINATES: protein motif:HMM:NF020893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HZK25508.1"
                     /translation="MFLESIGIPFASAFVALTAGTIISAGKASFLEVLLITTTGLTLG
                     SIVSYFIGYWGSSVGRLLLKDRKKNAEKSRFLEHYRRWGEVSILFAQLFGTTRTWASI
                     PAGAMKMKLQRFILYTAIGGTIYCTAAIGFSFVVTKLLAMLQLQYLALVLLSLVIVLA
                     GVIFFRLRDHQETHTKKEPKTPAK"
     gene            complement(22114..22296)
                     /locus_tag="VFC74_09025"
     CDS             complement(22114..22296)
                     /locus_tag="VFC74_09025"
                     /inference="COORDINATES: protein motif:HMM:NF019485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1657 domain-containing protein"
                     /protein_id="HZK25509.1"
                     /translation="MSDYRNLVENSIEKCQSSAADLRTASSQTANIAAKNSFTQAAAE
                     LEQCVQKCKIALKQLY"
     gene            complement(22341..23378)
                     /locus_tag="VFC74_09030"
     CDS             complement(22341..23378)
                     /locus_tag="VFC74_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25510.1"
                     /translation="MRNLPTLCIAVHDNMLAAALAVPEIRRQLSPQETLRARALRYQR
                     GNTPYPSWYLFGDHLKGLAGLVECYSRQAAVSLSPQSLLTAELTYDYLDTQFCTVDDV
                     LFAQLASKGIKIFGYLPEKFASKAYLAAALEENGGKRYLPLQQTYTKHSIAQLPAGEQ
                     AVWIVKTPFGSAGNNSNGTPYTVWLKDALQENLPSLLAALAKDDKLICSEFVITADPY
                     AQDADHVVHKMPFLANGAAARPYGRFCQRFIYHCNWQALQVQRKLALGEFVGKPEITD
                     GRVDSIHQFPRFVEKLAFQQGRVMLSVDFIIPPDGLPRYLETNKLAATFAEQFDPHLP
                     PLLDYYAQLPL"
     gene            complement(23375..23542)
                     /locus_tag="VFC74_09035"
     CDS             complement(23375..23542)
                     /locus_tag="VFC74_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25511.1"
                     /translation="MSYYPEPWKINDPDVEIAEDMSAMEALDPPYEPSGPNQESLLRR
                     YWERLVRGYHR"
     gene            23770..24501
                     /locus_tag="VFC74_09040"
     CDS             23770..24501
                     /locus_tag="VFC74_09040"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK25512.1"
                     /translation="MQLTVLGCYGPYPAAGQNCSGYLLQDDGVNVLLDCGNGVLSRLR
                     YHCQPGELDAVMLSHLHSDHSSDVMILRYALQINGRQNTESPLKVYAPPQPKEEYARL
                     AYKNYLQSQPLSADSKLTIGKLQFTFSKGVHAVPSYVITVTAEGKKLVYSGDTAYFPA
                     LVEAVQGADLFLCEANFLKSDLQLGNPNHLAAYQAAEVAKAGGVKQLVLTHHHPERDP
                     QQALAEAQAVFVNTTLARAGSLYRL"
     gene            complement(24508..25329)
                     /gene="thiD"
                     /locus_tag="VFC74_09045"
     CDS             complement(24508..25329)
                     /gene="thiD"
                     /locus_tag="VFC74_09045"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011194291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HZK25513.1"
                     /translation="MFKALTIAGSDSGGGAGIQTDLKTFAALGVYGTSAITALTAQNT
                     IGVHGIHAVPAAFVAAQMEAVLSDIHIDAAKTGMLGDAELISAVAEMLIKFNVRKLVV
                     DPVMVAESGDPLLPAAAVEALCKKLLPLALVVTPNLPEASVLLGREISTVEEMIMAAK
                     EIHRFGCRTVLVKGGHLPGEEMVDIFYDGDTIHRFAEPKLHTKHTHGSGCTYASAITA
                     ELAKGQQPLAAVQTAKRFITQAIAHGLAVGNGYGPTNPMAELLAVSRHRKGRTKE"
     gene            25487..26077
                     /locus_tag="VFC74_09050"
     CDS             25487..26077
                     /locus_tag="VFC74_09050"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HZK25514.1"
                     /translation="MNIQQILAQFEPICTPRLMMRKITRDDATQLYKLTSSPEMTKYL
                     HWYPSQSLSAVKVVIEQRLTEYKTGETRALVLAIADLHTNQLVGNIAYKLTDRAGLTC
                     EIGYWLGKEYWHQGYASEALKACLHYGFTVLGFERIQCCCSPENIASWRVMERAGMQC
                     EGILRNSYYLKGKLYDDKVYALLKAEYQALQAQATD"
     gene            complement(26178..27794)
                     /locus_tag="VFC74_09055"
     CDS             complement(26178..27794)
                     /locus_tag="VFC74_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25515.1"
                     /translation="MRLTGILTELTVRPHAMSEIRTLALILSVLYVSRALFTFFYRYL
                     SHLAAWRLVAEMRVIVYNHLQKLSMSYYNDKQTGQLMSRTTNDTATFEVLIAHAVPDL
                     IANLLILLGVTMILFVIHPTLALLTLIPVPILLYSGYFFTKRVRPFFRTAQVAVADLN
                     AVLQDNLSGMREIQVFNQQRKEKMRVAEKAAAHAQAILHALKLSALFHPAVEAVSAFG
                     TVIVVGFGGYLALQGNLSVADIVGFLMYLSLFYQPIATLARVTEDLQQGIAGAERVFE
                     VLDTEPDIQDHKNALTLTTSTGRVSFENVSFHYQADTPVLKDLTFTAQPGEMIALVGP
                     TGVGKTTIISLLARFYEPVEGTISLDDHNLKDLTLASLRNQISIVLQDVFLFNGSIAE
                     NIAYGVKACTPEEIREAAIIAKADDFIQEMPEGYDTYIGERGVRLSGGQKQRLSIARA
                     VLRNTPILILDEATAAVDVATEAKIQAAIQELAGTRTIIVIAHRLSTVKRADQILVLK
                     EGEIVEKGTHEDLLRQAGLYKSLCDVQFQN"
     gene            complement(27769..27912)
                     /locus_tag="VFC74_09060"
     CDS             complement(27769..27912)
                     /locus_tag="VFC74_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25516.1"
                     /translation="MKHVLRLVQYAKPYWWLLIITGVSLLGDNWPQPGSTISDYEVNW
                     DPN"
     gene            28209..29594
                     /locus_tag="VFC74_09065"
     CDS             28209..29594
                     /locus_tag="VFC74_09065"
                     /inference="COORDINATES: protein motif:HMM:NF014325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HZK25517.1"
                     /translation="MRVIIAGGGETGKALAVVLSAVQEVVVIESNRSRADWLAQHLDG
                     QVIWGEAQSLQTLLAAGTGGADLFVAATGVDEINLLACLTAKRLGCIKVAACLKNPDY
                     WEGQHLLNTELKSLDVLLHPVLLAAHAVVKQLINPSFVVNLSFANGRVALLALELTQN
                     SHFVGQQLQQLELPGQAVICAVTRQGETSARAKNFTLSAGDQIYVIGDALRKSHWLPS
                     SEQISPPQRVLIGDGSWLGQQIASLMGSSLQHMENVLLEADLVRCEELADGLPRSRIY
                     QGAITEHTFLAAIVNAKTDWFVAASDNDLVNILSALLAKNLGVRGMIVVARNQSAQVA
                     LKTAGINNIISPKTLVAEKVLSLLQRDNWHNFAIIGDGPVQAIEVVVQEGMPLVHKRF
                     GEDRLADGLIWGAMVRQGQLISPHTAERVQANDHLILLMPQGLSTTSSALLTVENVIV
                     KKKTSPRLSQS"
     gene            29591..31057
                     /locus_tag="VFC74_09070"
     CDS             29591..31057
                     /locus_tag="VFC74_09070"
                     /inference="COORDINATES: protein motif:HMM:NF014443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK25518.1"
                     /translation="MRWRGIFFLLGSVLLYLAFAMLVPLLWAMWQASATRLDFSITII
                     LTITVAWLFLRLGRPPTELRLQDAFAFVTLTWLFASLFSALPYYFSGCVPTLIDAYFE
                     AMSGFSTTGATVIISVESVDQAILLWRAMTHWLGGMGIIVLFVAVFPRLGVSAGLLLQ
                     AETPGPMTQTVTPRVANSAKILWLIYIALTAAQTIILNSVGFTFFEALTHAFATMATG
                     GFSTKNAGVASFDNPAAEWVIIVFMLLAGVNFALYYHLLLGNIRQVLKDEELRFYLIV
                     VIVATLLLFISLLPLPHAPLAALRHALFQVSSVVTTTGFANVDFDLWPEFSRMLLLLL
                     MFVGGCGGSTGGGLKQIRILVLGKYLLRELRKAIRPWEVAPLRIGDQVISQQLINRII
                     AFVCLYLLIFSSAVLYLTSLGCDFTTSLSAAASALGNIGPGLAAVGPMHTYAKLTAGA
                     KICLTLLMLIGRLEIYTVLSFLLPESWYRHKASWRQLK"
     gene            31109..31309
                     /locus_tag="VFC74_09075"
     CDS             31109..31309
                     /locus_tag="VFC74_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008977373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="small, acid-soluble spore protein, alpha/beta
                     type"
                     /protein_id="HZK25519.1"
                     /translation="MPKYKKKPEKMTLRELEMEKMKMEIAEELGLSDKVKEVGWENLT
                     ARETGKVGGVMSRRLRKEGKNK"
     gene            31438..32283
                     /locus_tag="VFC74_09080"
     CDS             31438..32283
                     /locus_tag="VFC74_09080"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HZK25520.1"
                     /translation="MRLGAHLSIAKGFDKTAHTALEIGANTFQFFTRNPRGGKARTIS
                     EQEITAWKELRRTYDIFQIVGHLPYTVNMAAKEDRPYNFAKMVITEDLQRMEAAGVEY
                     LVIHPGSHTGAGPEKGTERIVACLAEAFLPHHGQTMLLLETMAGQGSEIGTLAEIAEI
                     MEKLNFPERLGICLDSCHLTGAGYDFRQKEEVKRLVTDLENTVGISRVKAVHLNDSKF
                     EPGTNKDRHARLGEGYLGREGIINFVTHPVIKKLPIVLETPVDDYKEYGEEIALLRSW
                     LAEIA"
     gene            complement(32357..33421)
                     /locus_tag="VFC74_09085"
     CDS             complement(32357..33421)
                     /locus_tag="VFC74_09085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransacetylase family protein"
                     /protein_id="HZK25521.1"
                     /translation="MKSLFISGMAGSGKTAIALGLALKLREEGIKVGYFKPLDPTRGS
                     QNKSDDDVRLLKQVLNLPHDESVLSPVHTGPFQLSWGSNLETSTYRKKIMEAYEQVSV
                     GVDTVIVDGSLSPYTAASVGLDAISLAKMLSSAMVYVVRLTDDYSFDKTVFHNEHFRL
                     AGLPMLGNIFNNVSRTLLDKTKGVYTPILEKLGYPVFGVIPQKAEIAAPTVREYYDSL
                     GGELLAGDESKMMRLVEEVVIGSMTIEGALSYLRRAPNKAVITGGDRSDIAITALETS
                     TSVLILTGGLYPDVSVISKATEKKVPVILVHYDTYTAIERLHEVSRRIHPSDTHAVQI
                     AQDNISEYCNWKSILEYVKS"
     gene            complement(33436..35559)
                     /locus_tag="VFC74_09090"
     CDS             complement(33436..35559)
                     /locus_tag="VFC74_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008411974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HZK25522.1"
                     /translation="MSDLSILFNPSSIAVVGASKKEGKIGNSILANLQESGYTGHVYP
                     INPKETEIFGNKCYPSIKKVPDSVDVAVIAVPAERSINVVQECGEAGVKFLVVVTAGF
                     KETGEEGLKREHELVKICKKYKMRLVGPNVVGIMDNHTPFNASFTPGSPPQGKIAFIS
                     QSGAMLLAILDWSRSVGLGFSRFISMGNKADLNEVDFIWSACEDPQTSVILCYLEDVS
                     DGQRFLEVVREASKRKPIIILKAGTSSAGARAASSHTGALAGSNIAYDTAFRQCGVLR
                     ADNMADLFDLAIAFVNQPVPAGKQIAIVTNSGGPGIIATDSVERNKLEMARFSKETTA
                     KMRELLPAEASIYNPVDVLGDANADRYRVALEAVLDDESTQSALVLLSPADVTEPLKT
                     AELMVEMRQKYPQKPLFAAFMGGEGLAEGSRFLTHSGIPSYTFPEPALKAISGMVGYA
                     NMRQVIESAKPLYNFTDIDHQAVKATLYDVLRDHRLVLLGNEATQVAQAYGISVAPVY
                     LVNSPDEAIEKAESMGYPVVLKIASPKIIHKSDVGGVKVGLESAQEVKDAYVAIMESV
                     SRLMPGTPIYGIEIQKMMPPGNELIVGMSRDLQFGPLIAFGLGGIYVNLLKDVSFRLA
                     QSLTLHDIEEMITETKAYTLLRGYRGSTPSDIESLVQTIARIAKLSLDFPEITEIDLN
                     PVIAYPDSAVALDVKITVSYGDNLE"
     gene            36024..37160
                     /locus_tag="VFC74_09095"
     CDS             36024..37160
                     /locus_tag="VFC74_09095"
                     /inference="COORDINATES: protein motif:HMM:NF017221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZK25523.1"
                     /translation="MQDYNQLEQVSTKLLAAVVEGRKQIITLVKQARAEKCVMMAELV
                     ELKADILKVITQADRLEQDYKLARKHVLAVNGNFTKFTDQEIQIAYEEAQHLQNQLIK
                     MHEGEVSNQQQQKELEANLLELNEKLNKAEKLEGQTDLALQLLGGEIQNTNAKLDELQ
                     LRQQLSVRIIKAQEEERLRVAREIHDGPAQSMANVVLRAEICEKLMVLEPEKVKEELH
                     DLKAMVKESLQEVRKIIFNLRPMVLDDLGIVPTLRRFISELQKHAKLTIDLVVLGGEE
                     SRLPKTLEVAIFRIVQEALNNINKHAQAQRCIIKLEILPNRLNISVIDDGRGFDTQLF
                     TTTNTESFGLIGMRERVELLRGEMEITSAPGSGTEIQIIIPIKR"
     gene            37166..37810
                     /locus_tag="VFC74_09100"
     CDS             37166..37810
                     /locus_tag="VFC74_09100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK25524.1"
                     /translation="MQKTTILIADDHPLIRDGLKRILEFHEEFTVVGEAANGEVACTI
                     ARQCQPEVVLMDINMPQMNGIEATKQIKHDCAATNVIALTIHDDDEYIFELIKAGVSA
                     YVLKDIEADSLINTIRAVASGETVFDATITAKMFTEFQRQTRQNSLDEPLTSREAEVL
                     TLIARGRNNKEISSELYISQKTVKNHITNIFRKINVADRTQAALYAVKNRIVKL"
     gene            38024..38203
                     /locus_tag="VFC74_09105"
     CDS             38024..38203
                     /locus_tag="VFC74_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HZK25525.1"
                     /translation="MKEMVKRFISEESGQGMTEYALILALVSIAAIAALKVLGPKITQ
                     VFKEVNEELPTVPES"
     gene            38290..38682
                     /locus_tag="VFC74_09110"
     CDS             38290..38682
                     /locus_tag="VFC74_09110"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HZK25526.1"
                     /translation="MMGKWFKNQKGQAIVESAIVLPIILLLLLGMVEVGRVCNAYLVV
                     TQAARHGARYGAVGANDVEIADRIASVVTPLNPTDLTITITPAGARHTGEDLQIQVSY
                     PVQLITPLAGRVVSNPWLVKSAITMRVE"
     gene            38693..>39146
                     /locus_tag="VFC74_09115"
     CDS             38693..>39146
                     /locus_tag="VFC74_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HZK25527.1"
                     /translation="MRFNSFKRYLQNERGTAIVILAGAMTLLMACTALVADIGVNFVT
                     QSRLAVAADAAALAGATKFKDGAAQVQETALAIAAQNGIKAEDVIVNVAGSGKEVTVT
                     ATAPVQLFFARSFSKQEGDMAQSARVIAGRPTAMTNIVPLGINENANFE"
BASE COUNT        10898 a         8019 c         9531 g        10698 t
ORIGIN      
        1 gttatatttt ctgggtgcaa gtccagcact tgctgaactg actccttaac catcttttgc
       61 tcttcgcctg gcagaccgat gattaaatcc atgtttaaga tgctgatgcc agccttacgc
      121 acggcttcta cagcttctac aatatcagcg ggactatgac acctgccaat acgatctagg
      181 gtaatctgat gcatggtttg cggattgaca ctaatgcggt taataccatg tttaatcata
      241 acttccagat gctcggctgt taatgtctca gggcgcccag cttctatcgt aaactcgcgc
      301 cagctgccgg gaaaagcttg gcgtaaagcc agtataattt tttctagttc tctggggttt
      361 agggccgtag gagtaccgcc gccaaggtat actgtatagg tatgcatgcc aaaggcctgg
      421 gttagtgcgc ctgtcgcggc aatttcctgt agtaagcttg ttgtgaattg gcttcttaaa
      481 ctgccaagtt cagttagaga gtgcgaagga aaggaacaat acaagcaacg cgtagggcag
      541 aagggaatac caacataaac actaaaaagc tgtgggttag tactttcctc aagtattgct
      601 ctttcttttt gacaaacttg ccagagtaaa aaaaactttt ctttacttat ttttgttgcg
      661 gcgtgcatcc tactaaaagc agcctggtaa tctagctttt gatctattaa tttatggagc
      721 tgtttagctg gccgtacacc aactaaagta ccccaaggca tgtctggcac tgtgcatcac
      781 ctcttgctta ttatacacca tcagaactta tgtagtgaag tttaagcaaa gataatgctg
      841 gggactgctt actctgtgcc cttctcctga gccaaacagt ctacttcatg ttggagccgt
      901 tgtgttgctg ccggaatgac ataagtctga ataagcacaa attctaataa cttaagcagg
      961 acggctgcag tttgctgagt gggctttttc tcaaaagcaa aatagggcag aagctgctga
     1021 ttgttgccaa tagtggcggc aacactgttg acttgctctg tcaggttgac tttttctgcc
     1081 agctgtctaa ttaaagctgc tagttcttta acctcgccct ggcctaaatt cgttttaaag
     1141 atgtcttcca gagaggcacg caccattgtc gttgtcgctt tataatattg atggtgatag
     1201 gtctccagcg cttccagata attatcagtg ataggctggg gcacattttc tattcctggc
     1261 atcatttcac ggctctggtt aaatgaagga tagttaaaaa tttgactgtt ttcgtagtcg
     1321 cggcagttga ccagccaaaa actaacttta gctgcacagg cagggcaaat accaagaaat
     1381 gtgatagtat tagtagccgc atgatgaaca tagtcacgac aaaagtaggt aagataatcg
     1441 tgacaatgag ggcaagttaa agcaatagca gcggggacgg gcaacccgcg gaaggtcgtg
     1501 ttagctcgaa tcatattttt gctcaccaaa tagctcataa aatttccccc ctgttaagtc
     1561 tatgcaagac ggaaggcatc atgcttagtt caagctagag ctataaaagg gtttggtgta
     1621 acacacttta gtgtaaaaaa aaagagcttt acgctctgct taaaaaataa tggtgggcca
     1681 tcagggattc gaacccggga caccctgatt aagagtcagg tgctctacca actgagctaa
     1741 tggcccacaa aaggaaaagg tggcaagccg ccaatgattg ttgtggctgg ggcggtagga
     1801 ctcgaaccta cgcatccggg agtcagagtc ccgtgcctta ccagcttggc tacgccccaa
     1861 aagtggtgga gaggactgga ttcgaaccag tgaagtctga gacaacagat ttacagtctg
     1921 ccccctttgg ccaacttggg tacctctcca gattaaaatt gtctagctta gaaaaaagag
     1981 ctaatcagct ctaattagcg aatggtgcga gaggggggat ttgaaccccc acaggcgtat
     2041 tgcccactag atcctgaatc tagcgtgtct gccgttccac cactctcgca tttttaatgg
     2101 tgagccatcc gcgactcgaa cgcgggacac cctgattaaa agtcaggtgc tctgccaact
     2161 gagctaatgg ctcaaagaga aaggaaaagg tggcaagcca cctattataa acattggctg
     2221 gggcggcagg actcgaacct acgcatgcgg gagtcaaagt cccgtgcctt accgacttgg
     2281 ctacgcccca aaaatggtgg agaggactgg attcgaacca gtgaagtctg agacaacaga
     2341 tttacagtct gccccctttg gccaacttgg gtacctctcc acgttattat tatattggag
     2401 ccggcgatgg gactcgaacc cgcaacctgc tgattacaaa tcagctgctc tgccaattga
     2461 gctacgccgg catcgcttca gcgacataac atatagtaac atattttgca ttgcaagtca
     2521 acacttaggt attttttgag tttccgctct gacccacata tgataatgtc tcgttaagag
     2581 ttaagtgatt atgtcccgat ttgttttaaa attattgaga cattgtatgg taggatatag
     2641 atttgtgacc agacctcaga aggatatttg tagatgtatg tttttcttgt aaactgcgca
     2701 ttcctaggaa aggaaactgg attaaggagg attaatattt catgaatgac aacgagttta
     2761 tcaaaatatt taatgatttg gaacgtgtag aaaaagaatt gcgtaaagta ggtaacaatc
     2821 aagaacagcg gcaaaagtgt tatgaccgac ttttagagtt gcgtcgtcgc atggaccatt
     2881 atgtggaatt ttggctgcgt tttgaggaaa aaattaacga aatgcaggag gaatatgact
     2941 ttttactgcc tgacgaaatg ccggagtctt ttctgcaagc atttggtacg atcttaccgg
     3001 aggctgatgc gcacgagatg cagacagaat tagaagaagt aaagacagat ttagacagga
     3061 gaggagtaaa accattgctt ttacaagggg ataatgaagc atgtattcgt tcatttaggc
     3121 ggggcttagg ttttctggaa ctagcaatga tggatgaagc aattcaagaa tttcgtgagg
     3181 taatccacca ggaaccagac ctattattag cgcatgtgtg cttgggggta gcttacgctg
     3241 aacggggcct ggctgatgaa gcaatgcgcg aattacgttt ggtgcaagca ctgacagatg
     3301 atcagcaaac cagagtagtt gttcacaatg ccttgggtaa tgtctatgcg gataaggaag
     3361 actatgaaca ggcaaaaatt gagtttgcga aagtgatcga gctggaccca gactttacca
     3421 ttgcccattt caatttaggg gcaatctatt ataatcttaa gatgtatgaa gaaagtatcc
     3481 aggaatttca atttgtcaag aataagtttc ctaaagattg ggagatctat ttttacttgg
     3541 gcttaagtta ttgtaaggtg gataatgcag aagaagcgtt ggttaattta ttaaaagcct
     3601 cttatctcgt accgcataat ccctacgtag cttttgaatt aggcttacta tacgataatt
     3661 taggcgaaac ccggcgtgct ttagaatgct attatcgctc ccgcaaactc tatcaggaac
     3721 aagaagtaca actttaatcg ttgctgccag ggttggctaa gcaggaaaaa tcatcctgat
     3781 gaagaagttt aatggcacga agtgaaggag gagcgtctta gtgagcacta ttcgtgatgc
     3841 gaatttagca ccatcaggta aattgaaaat taactgggta cgggagcata tgcctgtact
     3901 aaatgaattg gctcagggtt ttcggcagac taaaccattt acaggtttac gtatcgccat
     3961 ctgtttacat ttagaagcga aaacagctta tatggcgaaa gtcttacagg aaagtggggc
     4021 cgaagttacc atcaccggca gcaatccact ttctacacaa gatgatattg cggcggcgct
     4081 ggtagcagat ggccttacgg tacacgcctg gcatggcgca acagctgcgg aatatgatga
     4141 tcatttacat aaaaccttac agagtgagcc gcatttaatt attgatgatg gcggcgactt
     4201 agtaacaatg ctgcataccc agtatccgca gcttttgcct caagttagag gtggtgcaga
     4261 agaaactacc accggggtaa ttcgtttacg ttccatggcc gcggaaggtg ctttacagat
     4321 accgatgatg gctgttaatg atgccaactg caaatatttg ttcgataatc gttatggcac
     4381 tggacagtcg gtctgggatg gaattatgag agcaacgaac ctagtggtgg cgggtaagac
     4441 tgtcgtagta gctggttatg ggtggtgtgg caagggtgtc accatgcgtg cccgtggttt
     4501 gggggccaga gtaattgttt gtgaagtcga tcccattaaa gctgtggaag cagtgatgga
     4561 tggcaatact gtgatgccga tgatcgaggc ggcagcacat ggtgatattt tcattacagt
     4621 aaccgggtgt cgggacgtta ttactcacga acattttgtg agaatgaaaa gcggtgccat
     4681 tctctgtaat gcggggcact ttgatgtcga agtaaataaa ggtgatttac gagagttggc
     4741 tggagaagcg cagctggcac ggcataatgt ggaggctttt gaatttggcg aaacacgact
     4801 ctatttactg gcagaaggtc gtttggtaaa tttagcagct gccgatggtc atccggctga
     4861 aattatggat atgagttttg ctttgcaggt agaagcacta cattatttga atgtcaatta
     4921 cggacagctg cccaatgagg ttttacctct gccgcagtct atagatgata aagtagcacg
     4981 tcataaactt gctgccctgg gcatgcgtat cgatcaactg acgtcagcgc aagaaaatta
     5041 tttaagaagc tggaaacagg aataggggga ggaaaatgac tattcactta gcgataattg
     5101 gcggtacagg tgtgtatgat cctaaactcc tgggagctgt agagagatta gaagtaacca
     5161 cacgttatgg cacggctctt ttaactcaag gcatttatca aggggaaaaa gtagtatttt
     5221 tagcacgaca cggcatagag cacggtacac cgccgcataa aattaactac cgtgctaaca
     5281 tggctgcttt ggttaaatta ggagtaacta gggtaattgc cacagcggct gttggtactt
     5341 taaatgagac catgccccca ggctctatgg tgctcttaga acaattttta gattttacta
     5401 aaggacgtga agctacattt tttgatggtg gggaagcagg ggttgtgcat ctcgacttta
     5461 cgcaacctta ttgcccacag ataaatcaga gtttacagca ggcagcgcaa gctgctgggc
     5521 tggaattatt acagggtgga gtctatgtct gcacagaagg accgcgtttt gaaacaccgg
     5581 cagaaattaa aatgtttaag ctgctgggtg gtgatttggt gggcatgact aatgttcctg
     5641 aagtcgtatt ggctcgtgaa gcaggtcttt gctacaatac agttgctttg tgtacaaact
     5701 ttgctgccgg tcttgccccg caggagctta cacatcagga agtactggat gtgatggcgg
     5761 aaaacgtgga taaagtacgt cgtctcttgg ctgaagttat cccaagattg ggaactgaac
     5821 ggtcgtgccg ctgtcaaagt ttagggcatg aaattttact ataggtgggg aagatgaaac
     5881 cactagggat aaaaaatcaa cagctattgt tgttagatca gcgtcttttg cccactcagg
     5941 ttgagtatct tacctgtgag aattggcaga gtgtagcaca ggccattaca gcgatggtag
     6001 tgcgaggcgc cccagctatt ggcgctgcgg ctgcctatgg tctggcgatg gcggccacaa
     6061 gtttacaggc agcagcgccg gcacaatttg cagctggctt acaagaagcg gccgctggtt
     6121 tacaggcggc acgccctacc gctgtaaatt tagcgtgggc ggtaaaacgc atgctgaacg
     6181 tggcccgggc aagtaccagg ctggacgcga aagatatagc cgccagacta tggcaggaag
     6241 cagataaaat tgccacagag gatgtggcgg ttaataaaaa gattggtgag tacggtgccg
     6301 ctttaattcc ggcacaagca cacattctta ctcattgtaa cgctggggcc ttagctacag
     6361 taggttatgg cacagctctg ggagtaattc gcagtgctgt agcacaggga aaaatgatcg
     6421 ccgtttatgc tgatgaaact cgcccctatt tgcaaggtgc acggcttacg gcgtgggaaa
     6481 tggtacaaga acagattcct gtaacggtaa ttgccgatag tatggctggt tttcttatga
     6541 agcagggaaa agtggatcta attatcgtgg gggcagatcg tatcgcggct aatggcgatg
     6601 cagccaataa aataggcact tatactttag ctgtcctggc caaggctcac aagatcccct
     6661 tctatgttgc cgcaccagct tctacatttg atttaagcct tacagacggt gagcaaatac
     6721 ctattgaaga gcgcgacgag cgtgaattaa tcttcttcgc cggcaccagg attgccccag
     6781 aaggggtgct gtgctataat ccagctttcg acataacacc ggcagagctg attacagcaa
     6841 ttatcacaga atatggcgta atcgaacggc ccacagcggc cgctattgct gccgtattag
     6901 gagaaaaaat atgaaggatt gtcagacgga aatactacag accgtacact cactttgcca
     6961 ggagcattta gtactgggca cttggggtaa tgtatctaca cgcgaagcac agtatttttg
     7021 gattactccc agcggtatgc cctacgattg tttaaagcat gatgatttaa ttaaaataga
     7081 tttagccact ggccaggctg ctggaccgtg gaaagcatca acggaatggc ctttacatgc
     7141 cgctatttac cgaggacgac cagactgtgc tgcggttgtc catacgcaca gtgtttatgc
     7201 cacagctttt gccgtagcga atcttcctat tccaccagta gttgaggatt tggtgcaaat
     7261 tgtcggcggc ggtgtggccg tggcagatta tgctttaccg gggagcaaag aattggcgca
     7321 gcaggcacta ctggccttag ccgacaacaa tgcggtgctc ttagccaacc atggcctggt
     7381 tggtatagga aaaagcctca ctgaggcgct gaccgtttgc cggattgtgg aaaaaacggc
     7441 ccaggttgta attcaagcta aagcgttagg gcctattcgt gaaataagtc agtcagatat
     7501 taaagtaatg cgtcaatttt atgtgactgc ttatggacca ggtcataaaa gggaggagag
     7561 gccatgagaa cacttattca gaagacaaca gttattacat ctcatactca acctgaaatt
     7621 ctcgaggatg ctgatgtagt aattgatgga cagatattta gctatgttgg cccaagccaa
     7681 gcttggcagg aagacttttc gcaaataata gatgggcgag ataaagtagt ggcccctggc
     7741 tttgtgaatg cacatagtca tgctgccatg tccctgttac gctcatatgc tgatgatgtt
     7801 cctttgatgt actggctgga aaagcgtatt tggcccattg aggcccgctt aaaaagagaa
     7861 gatgtttatt ggggaacaaa gttagcattg gcagaaatga ttaaaggcgg caccaccgct
     7921 tttgctgata tgtatttctt tatggagcaa gtagctgaag ctgtagaaga agcaggaatg
     7981 cgggcggtgt tatgcagagg tttggtaggt gtggggcaag aagctgaatc tggtctggaa
     8041 gaaagcaaac attttgtgca gcgttggcac ggtcgggctg atggcagaat tacgactatg
     8101 ttgggtccac atgctcccta tacctgtccc ccagactatt taaaacgtgt gctagcactg
     8161 caagaggagc tgcaggtacc aatacatatc catctagcag aaacgacaga tgaggtagaa
     8221 gatattcgca agcaatatca gctgacacca gtacaattac tcaaggatgt tggtcttttg
     8281 gacagacctg tattggcagc ccactgtgtc catttaactg aagacgatat ggatattctc
     8341 aaagcctatg atgtgcatgt ggcacacaat cctggcagta atctgaaact tggcagtggt
     8401 gtggcacctg tacctgattt actgtcccgt ggcatcaccg ttggcttagg aactgatggt
     8461 gcggctagta ataataattt agatatgatg gaagaaatga ggttagccgc cttactacat
     8521 aaaggtgtac gcatggatcc gactgtgatt actgctgcgg aggcactaat gctcgccact
     8581 agtgagagtg ccaaagcaat tttcttggat caggtaggta agattacgga agggatgaaa
     8641 gccgacttaa tcttagtaga tttacatcaa ccgcacctaa ccccacgaca taacatcatc
     8701 gctcacttgg tctatgctgc tcaaccttca gatatttcga tcgtgatggt agatggtaag
     8761 gtgctgtgtg agaatggacg tctaaccaca cttgacgaag acgaaattat ttaccaagcg
     8821 gaacagcgtg ctcagttctt agtcaaagaa gaccaggaaa acaattaata agcccagcag
     8881 cagccgggct tatgtagcag atttacttat tgttaccact ttcagcacgc atacggctta
     8941 acacatctgt aatttgatca aagaatgtag aaatcggttt gccgtttcta ataccacgcg
     9001 agatatcctg cagctgtttg acgaggtccg ggttcgcaga aacataagca ttgacaatac
     9061 ttggttcccg atcttcaatg cgtcgagcta cttcttgctt aagttccttt tcaccacgct
     9121 gcgctgtttc agcgcgctcc aaagtaatac caaccagtgc catattggaa atgaccacaa
     9181 ctactgaatt atcaacgcct tgcacaccag tggcgatgtc agcgattctt tctgccatct
     9241 gcgtagcatc tggggtgggc tgacctggcg gctgttctct tggtgtttgt ggctgcggcg
     9301 ccggcggtgg gttaacgcct gggttgggtt gtggtgtttg attaggcgct aaatttgggt
     9361 caggcacagg tgttggtaca ggctgtcgtt ccggttcagg gcggcggaag agcgaacaac
     9421 tgctcacgaa caaagaagca accagaagca gggacaggaa gtaaactaat ttttttgttt
     9481 tcattgtctc acctccttgc tctgttagtg tttgagcctg cagttttttt atactgtatt
     9541 aagttgcatc gcttgccaaa taataagttg agcttacttt atttaccaca taaataagta
     9601 gtaataataa caagtggagg gatggcatga gagttttagt cactaatgat gatggcgttt
     9661 atgctgaggg aatacaagca ctagcacagg agataacaaa aattgctgaa acatatgttg
     9721 tcgctcctga tcatgagcaa agtgctactg gtcacgctat tacgatgcac cgaccgctgt
     9781 tggcagaaga agttaaatat caccatttgc cagaattatc accgtggaag gttaatggta
     9841 cgccagccga ttgtgtgaag ttggctgtag aagcactgct gccgcataaa ccggatttag
     9901 ttatctccgg catcaatcgt ggtgcaaatc taggtactga cgtactgtat tcaggcactg
     9961 tttctgcggc gatcgaagga gtagtgctgg ggattccagc aattgccgtc agtctagctg
    10021 aataccatga tccgcactat gaatatgcag ctgcatttac tgctcgtttg gcgaaacagt
    10081 ttatgcagca aaagcaagag ggcgaaatgc tcttaaatgt taatgtgcct ggatgtgccg
    10141 ctgaagaaat acgcggtgtc gctgtaaacc gtttaggtgt acgtcactat aaaaatccgt
    10201 ttgagaaacg tacagaccca cgcggacgct cttattactg gttagcagga gaaatagtag
    10261 atttagaagg ggaaaatgat acggatgtgg cagcaattaa agcaggtaaa atatccatca
    10321 cccctattca gcatgattta accaactttt tactgctaga taaagtaagg gagtggctaa
    10381 ctgacatgaa gggtaggaac taattttcta aaccgcaatt taaaagcata taatttcatg
    10441 actttctcca tctatggggc ataaagatag tatcgtgaat agttggttca acaaaatgcc
    10501 agtatggttg aacaaacctt aatatactgg aatttgcaca aaaggaggaa tgaggatgcc
    10561 gacatttttc ttttacggac ccagtttaga actagaaaag aagagaacaa tgattaaaga
    10621 aattaccgcc gctgcaagca aggctacagg aataccggaa aaatctttta caatttattt
    10681 gcgtgagacg cagcatgaga atgtaagcgt tggtggcaaa ctactttcgg aaagagatta
    10741 aggaagatag aacaacttgt ttacggacaa gttgttctat ttttttagct agagacatag
    10801 aaataaatga gaaatcttgt aggggatgat gttgatgaga ctggctcctg tggaaaataa
    10861 gccagaaaaa cagcatatat ttttacagcc gtactttatt atcactttcg ttgttgtggt
    10921 tttaagtagt gtgtgtgtat ttctattaac gcgacagaaa gaggaagcag tacctaccat
    10981 gctagatata gtagcagaaa ctaccgcagc ggccgcggcc cataagcatt ataatcttgt
    11041 aattgaagaa agcgggccgg ggtatttact acgttttagt ggtcaagtac atgatgaccg
    11101 cctttatggt aaaattgatg gttacgatct ggagatattt gcagatgaag atcagttttt
    11161 tattaaaaat tcactggcgt cagagtggca ggatataaaa acggcgaagt tagaaacctt
    11221 acccacttta atacgtgacc cacattattt attagcaaat ttgatggctg gtaaagaaat
    11281 agccatcgaa acaggaacgc cgcggacagt agatgatgtg atctgtcaga cttactttct
    11341 ggaaataccg ccaccaaact tgcagctgtt aacacgtttt tccacagatg ctacactaga
    11401 taaattgcag gtctatctct ggtttgctga agaaagcggc tttatgcatc ggctggcagt
    11461 gatgatgaat gtcactgtgg ctgaggaaac tatccaaatc aatcgtacct ataacttaag
    11521 tcctgaggct aaagattttc ctgaagatat acccaaaatc aatcgtaaag taatgccgat
    11581 ttaatccttt gtagaaaaaa aatgatactt tttggttttt ttttgtcagt ttaagccata
    11641 ttagttacca aagcaaattt ggagggctaa gatggctttt attatttggc tgctcggtct
    11701 tgcggatgtc tatctgacca aatatggcct gcaaattggt gtaattacag agggaaatcc
    11761 tataatgggc tatctattta acctaaattc ccattgggca atcatttttt ccttaagctt
    11821 atctggtttg ctgttatatt gtctgcagag gctgtgctta cgtacaacaa tggcggaaaa
    11881 agcgatgaag gggttacttg ttgtacgaat attggttatc ggcttacatc tcaactggct
    11941 ttatcaatat tattgtcttt agaaaggaat tgccaatttt gtgtcgaaat tactagcgta
    12001 atgaaggagg agagctagaa tgttttacta tgtatgttgt cctgaatgta atactgattt
    12061 aagccagcat gttgatgaag aggatcccac tgaaagcatt gttatttgca gtcattgtaa
    12121 aaccgctcta caactacaac atgtcgacca atgggatgaa gatatgggct ttgacgtgac
    12181 cttattctgg tttgaaaaag tggcagaagc atagcaaaaa gccgtggtta cccacggctt
    12241 tactatttgt taaggctatt tttttaaaat ggcaggggag acaggattcg aacctgcaac
    12301 cgacggtttt ggagaccgct actctaccgt tgagccactc ccctgcggca taatctattg
    12361 tagcttagat tggacatttt gtcaatatgc tggctggctg cggacaatct tgaacggtga
    12421 cagtcgtcct cttaacgccc tcaacaggat gagaaatata atatgccttg cttaaatgca
    12481 gtgcctgtta taaaatataa agaaacttta gtgattgttg tggggggtga gacaatgcgt
    12541 gagcacatag gtttgattgg tattggcgcc atgggtagtg cactgctgaa agggttatta
    12601 agttcaggga tagaaccgga taaaattatc gttgctgatg cagcagaagc aaaactgcag
    12661 caggccgctg taacctacca agtattgatt ggagaggttt gtgaggtagc caccgctgcc
    12721 agggttttat ttctagcggt gaagccgcaa gatatgcctt ccttgctctt acaactatca
    12781 ccgctgctgc agcaggatca ggttgtggtc tccgtggccg ccggtgtgac catccaggca
    12841 attgaagaaa atctgtcggc ccaggtcggt gtgattcgcg taatgcccaa tactccttgt
    12901 ttaataaatg caggtgtcct agccattagt gccggacacc atgtacagga agcagatata
    12961 catctagtag agacctggct aaaaaatatg ggcagtgtct atttagttcc agaaaagctg
    13021 ctggatgctg tgaccgcagt aagcggcagt ggtccagcct atgtttattt gtttttggag
    13081 gctttggccg atggcggcgt cttagctggg ctgcctagag ttctggccca agagttggcg
    13141 gtggagacag tgcttggtgc ggcgcaaatg gttaaagcaa cagctcaaca tcctgcgctg
    13201 ctgcgagaaa tggtgacctc tcctggaggt accacggcgg ctgctctgtt ggccttagag
    13261 cagggcgggc tccgaggtac gctgcagcaa gctgtaaaag cagcagccga gcgctcaaag
    13321 gagctgggaa gttaagtggc aaataacagg ggcctgctta aaaataccca aacaatagtt
    13381 gtcaaggttg gttcaagaac attaactcat gccacaggca aacttaatct gcgcatgttg
    13441 gagaagattg tgcgggaatt agccgatcta gctaaccaag gccgccaagt aattttagtg
    13501 acttcggggg ccgtcgctgc cggtattggt cgcttgggtt tgtcggccaa gccgcagagc
    13561 atgccggaga aacaggctgt tgctgccatt gggcaaggtt tgttgatcca aatgtacgaa
    13621 aaactatttg ccgagtatgg actgatagcg gcacaggttt tgcttacgcg agatgatgtt
    13681 aatgatcgcc agcgttattt aaattcccgc aacactttac aggctttact tagatatggg
    13741 gctattccca ttattaatga gaatgatacc gtcgcggtag aagaaataga gttcggtgat
    13801 aacgacactt tatctgcgat ggtggccagt attattgctg ccgatctact agttttactt
    13861 tctgacattg atggtctata tacggctaac cctaaagttg atgcttctgc tcaaagagtg
    13921 agttatgtgg aagaagtgac cgcgaaaatt aaagcactcg ctggggaaac taatgaaaca
    13981 ctggctaccg gtggcatgat tactaaatta acggcagcag agatagcagg caattcgggt
    14041 gtgcaaatgg tgattgccaa tggccgagaa caaaatatct tacaaagaat cctagacggg
    14101 gaagatgtgg gcacattatt ctgtgcacgt gataagatgt tagaaagccg taaacgctgg
    14161 attgcctatg ggcagcagcc gcagggctct ctgttggtgg atgctggtgc caaaaaagct
    14221 ttattggcag atggtaaaag cctgctgccc agcggcattg ttaaagtaaa cggtactttt
    14281 agtcaaggtg agctggtagc gatcaaagat ctagctggta aggagttcgc gcggggttta
    14341 gttaattact gttccgcaga actgcagacc attcaaggcc tccgtacaga acaagctgca
    14401 gaagtgctgc aaaaagactg tggggaagca gtacatagaa ataatatggt ggtcaatagt
    14461 tagttggctg ctgtgaaaaa aggagttgtt agcatgagtg aggtagtccg taaagcggca
    14521 gaagctaaag ctgcagctgt gcatttagca atggcctcta cagtgaaaaa aaatgaagcc
    14581 ttattggcca tcgcggcagc attgacagca caacaggcag aggtactggc agccaacacc
    14641 aaggacctag aagcatttca ccaacagcca ggctatacga cagcgttaca tgatcggtta
    14701 gctttgaacg aggagagaat tgctggtatg gtcgaaggtt tgcagcagct ggccgctttg
    14761 ccagacccga ttggcgaagt taccgggatg tctgtgcgac ctaatggttt gcaggtcggt
    14821 caggtaaggg taccattagg tgtagtcggc attatttatg aagcacggcc taatgtcact
    14881 gttgatgcag cctctcttgc tctgaaagca ggcaatgcgg tagtactgcg cggcggctcc
    14941 gaggccatta attctaacaa agcattggtt aaaatactgc ggcaggctgt agaatcagtg
    15001 ggattaccag cagcagtgat tactattatc gaggatactg atcgtgcagc tgtacacgag
    15061 ttgatgcggt taaacgagaa tttagatgta cttattcccc gcggtggggc tgctttaata
    15121 aaaactgtgg tagagaactc cagcgtgccg gttattgcca caggaacagg aatttgtcat
    15181 acttttgttg atgcaactgc tgatgtggcg atggccacag caattgccat taatgctaaa
    15241 acaaccagac cagctgtttg taatgcgctg gagacattat tggtgcatca aagggtcgcg
    15301 gatcaattcc tgccccacat gctgccgcaa ttttttgctg caggtgtaga agtacgtggt
    15361 tgtgaaatca cgcagcagta tgatcagcgg gtcgctgcgg cgaaggaaga tgactggtcc
    15421 actgaatatt tggatttgat tatttcgatt aaagttgtgg catcattaga tgaagcaatc
    15481 aaccatatta aacgctatgg taccggacac tctgaggcta ttatcacggc cttgtatgat
    15541 aatgcacgtg agtttcaagc acgagttgat gcggccgcgg tctatgttaa tgcttccacg
    15601 cgcttcacag atggttttga gtttgggtta ggtgcagaaa tgggcatttc tacacaaaag
    15661 ctgcatgcca gaggacccat gggtctgaca gccctaactt caacaaaatt tattattaat
    15721 ggtaatggac aaattcggta accattgaca gcgatgctgg gtttggttaa aatgttagca
    15781 atatagtaaa tctttcacaa gcagtcctag caactgaggg cttgggctag acacaaaaat
    15841 tagcagccta tttaacagtg tatgtagttg aaacagagga ggcacgaaat gaaaaggttt
    15901 ttagagggtc ttgccaataa ccaagtaatg ttagttgata caaccttgcg tgatggtgaa
    15961 cagacagctg gggtcgtatt ttgcaataaa gaaaaagtgc ggattgcgca aatgcttgac
    16021 accctgggtg tacatcaatt ggaggtaggt atccccgcca tgggcggcgc agaaaaggaa
    16081 gcgattaaaa gcattgtcga cttgggttta gacgccagta ttatggcctg gaatcgtgcg
    16141 gacgtcaatg atgtgatgaa ttctttagat tgcgggacgg atgccttggc tatatccatt
    16201 tccacctcgg acattcatat caaatataaa ctgcaaaaaa ctcgtgaatg ggttttagac
    16261 agtatggcta aagctgtcac cttcgcgaag aagcacgaca aatatgtttc tgtgaatgcg
    16321 gaagatgcat ctcgcagtga gatggagttc ttactgcaat ttgctaaacg tgctaaagag
    16381 tgtggagcag atcgtctacg tttttgcgat acagttggta ttttggaacc atttaaaacc
    16441 tatgagcttg ttgaagagct gcgtaccact tcgggcatgg caattgaaat gcatacgcat
    16501 aatgactttg gcatggctac ggctaactcc ctagctggtg ttaaagcagg tgccaccatg
    16561 gttggggtga cggtcaatgg tttaggtgag cgtgctggta atgctgtact ggcagaagta
    16621 gcgatggctt tgaagtatct gtatggtatt gatgttaaag taaacccccg cgatttacgt
    16681 gagctttctg aatatgtcgc gcttgcctca gggcgtgagc tgcccgttaa tattcctatt
    16741 gtgggcacaa atatgtttgc acatgaatct ggtattcatg ccgatggtgt gattaaaaac
    16801 cctgcgacat atgaagtgtt tcaaccggaa gaagttggtc tgcaaagaca aattgtgatt
    16861 ggtaaacact ccggtagtgc ggcgattgtt gccaagttta aagaatatgg cattactttg
    16921 gctcataaag aagcttcaga actgctgccc caggtgcgtt ccgctgctat tgatttaaag
    16981 cgtactcttt ttgataaaga gttaatgtat atttatgagg aatataaaga caagaataaa
    17041 tgtaagggtg cataaacaaa gtttagacac cggtctgctg cagcccggtg tctgattctg
    17101 tatatcaatc ccgcagcaga aaggggtgaa gttgtgaagc tttcccaact atttgcgcat
    17161 tatccactgc catcggccag ccaaatagat gtcgaagtac aaggtattac ccatgactcg
    17221 cgctacgtta agcctggtga tatctatgtc tgtttagaag gtctacgcgt tgacgggcat
    17281 ctttttgccg gacaagctgt acaaaagggt gcagttgcgg tggtggcgac gaaaccactt
    17341 tatctgccag tgcctgtagt ttatgtaagt gatacacgtc atgctttgtc atttttagca
    17401 gataaatatt tcgatcaccc cagccgtaaa ctgcatgtgg tgggtgtatc gggtaccaat
    17461 ggtaaaacaa ctactaccca tttcttacaa gccatttaca aagcagcagc acgcccttgc
    17521 gctgtgattg gtacagttgg catccggata gataaagagt acttatccgg tggcctgacg
    17581 acgcctgagt cttttgattt gcacagaacc tttcaggaaa tgctggagcg gcagcttcgc
    17641 tatgtggcca tggaggtttc ttcgcattcg ctaacctggc agcgtgtaga acatgtagaa
    17701 ttcaatacag taatttttac taatttaagc catgatcact tggattttca taagaatatg
    17761 gaagactact ttcaggctaa agctcacctc tttgcattat taaagcaaga agagagtggc
    17821 cgaccacagg caattatcaa tgctgatgat ctttatggta aacgactgct gaccatggct
    17881 gatgtagctg tactatctta tggcttacaa gatggtgccg atgtacgggg cttcattacc
    17941 agtactagtg taggttccac cttagtactg gtacgttacc agaatctaga gtttgaactg
    18001 aatgtccact tgcctggcga atttaatgtt tataatgcct tggcagcagt tgcggccgct
    18061 ttagcagaag gcatagcgcc ggagcaaatc agtgagggta tcgataacct tgaagctgtg
    18121 cccggccgtt tagaggccgt taattaccaa caggagttta atatctttat agattttgcc
    18181 catacgccgg atggcttaga gaaagtactg caaacattaa gtgaagtgcc acaccgtaaa
    18241 ttaattacct tatttggctg ccccggggat cgcgaccgta ccaaaagacc ggtaatgggt
    18301 cgtattgccg aagtatacag cgatgtagtg gtcgtaactt ctgataatcc agcatccgaa
    18361 tcaccggaag agattattcg cgaaatttta gcaggcatgg aacatttgcc tgtagtttta
    18421 cctgaccggc aagaagctgt acgttacgcc ttatcgattg cgggaaaggg cgatattgtg
    18481 ctgttagcgg gcaaaggaca tgaaacatac cagctaattg gtgatcaaca tattccttat
    18541 tctgataaac aagctgtgga aacattcttt atccagtaac aagcaagatg tttataaatc
    18601 agccacttta cactagtgta aagtggctga tttgttaatg caaaaattat tggtggcgga
    18661 agaggcgatg gagtaaggag cgatagcggg gcaggccggt ttgccataac cagtagaaag
    18721 caggagagag aggtttatcc cattcgccga taaaggcaag atagtcgccg ccaaagcttt
    18781 gcttaaattg gtaaagtcca aataaaggat ggtcttcctc gacgttgccg ggtacaccat
    18841 aaaagtcaaa ccaaacggca cctttacgat gtgcccactt aatgcgctcc cagttcatgg
    18901 cgtgataggt atagagctta cgatatttat tagtttgtcc accataaaca gcccacgcct
    18961 tgtcacctaa ggcaaaggta agagaggaaa taacaggctc cccttcaata taacctgtgc
    19021 acagcaagac gcggtcgtct ttttttaatt catcgtaaac gcgttgaaaa tactcagggg
    19081 aacgcgtcat aatatcattg cgctcaccag tttgggaaag agctgtatag aagtcggcaa
    19141 tgcttgtttc ttcgttactg cggaaagaaa ggccgttttt ggggccatat cttattttat
    19201 aacgcatttt tttagtgaaa cgagcgaaaa tctcatcctc actgcctgtt aagggcaggc
    19261 ggaaggtgta gcgtggttgg gtgccgccaa actcatggtc cgtaccagtt aaatgaaaac
    19321 cgttatcttg cagatcctgg ggtgataaaa gaccttcagg aatggctgga tctatcttaa
    19381 tgacaatggc accgtgttgt cgggccaaag ttgttaaggc ctccagagta taatgaatta
    19441 gttgggagtc tgtcgtgtct gctaaaatcg gacccctggg tacataagca attgtacgat
    19501 taattagtgg tatgcgtcgc aagagaatag acatcgcagc acaaatttgc tggttatcgt
    19561 cttcgacaac agcacggact gcttcccagt ctgttttttt gatttcgccc caactgaatg
    19621 tttgtaacat atcaccataa gcggattttg ctaagaactc atcgtaaagt tccgccccgt
    19681 cgtgatcaac tatgcgggtt tgcatctatt ttccctccag tatgcatttt gccatttgaa
    19741 gttgttgagc tcaataaaac ctttttgtcc ctgtaagtgg tctagagagg tatgatcatt
    19801 tgtaactgca gttcgcattc cggacagtat aattgatgca gacaaccatc ctgtccgcgc
    19861 agctgactgt tttcttcata gggtgcataa ggccccagag cattttgtaa agcaccgtaa
    19921 tcggccatct tccctccaca agcagggcaa gtaaaatgca gttcttctaa accattacag
    19981 tatgggcaaa tatgtccaag cattagcata accccatttt ctttttagtt tgccctgcac
    20041 tggtctgagt aaacgcgggg ttatatttga actttctttt gttttcgcaa ttgttaaccg
    20101 tggcagctgg ttaataaact ctgggtgcac attactgctg ccaggctctt gctgcgggaa
    20161 gaataagggc cagcggcgtg cgtagtacgt tgagatcccg cagggggttt ccgcttagca
    20221 ccaataaata cgggtttttt cctggcgtga tactgcctaa ctgcgtaagc tgacaggctt
    20281 gagcagctaa ggaggtagct gcttgcaaag cgttgaagtt tgttaagccg gcattatgta
    20341 aaagtagtat ttcttctggc agtgctgtgc catgtaagac gccaggtgct ccagcatcag
    20401 tacctgctgc tatttttgct cctagtgctg ccccttcagc aattaattcc tgatgacgta
    20461 gtaatgagcg caagatgacg gtgtgcatag agggggtcag agcggtaaac agctccggct
    20521 ttgtcagctg agcattaacc ggggagagcg ttggtatcca acaagtatcg ctggctgcta
    20581 gttcttgcag tgttgctcgc gacataaaat agccatgttc gatggtggta acacctgcct
    20641 gcagacagcg gcgtacagct tcatcaccac tggcatgagc cataactggt aaatgatagc
    20701 ttgctgcctg gttaacaatc tcggttagct cagcggcact aaaagtggcg gcaccagttt
    20761 tgccataggt ggaaaaacta aagataccag aggcaatcac tttaatttgt gctgcacccg
    20821 ccgccgcgag cttgtccacg gcttgtaaag cctcccgcag gttggctgtg gctattccta
    20881 aggaggagcc ataagaacct tgctggtaaa ttgcgttaat ggactgcagg tgcagtagtg
    20941 gagtgttggt tgtgctgaca ctgagggtac tgcctgcatc acgtactgca gctaccccgg
    21001 cagctagata ctgctgtgcc cgagtggtaa aaggcgtagt gtcttcttca ggaaaagcta
    21061 ggtgaatatg agcatcaatt aacgctggca gtatggtggc ttgactaaaa tcccacactg
    21121 gataattatc atgagaacta ctatttacct cagcaattac ccctccatta attaagaggg
    21181 taccgctgtt atgtagattt tgtgaaaaac catcccaaag ctgagcatat tttagagctg
    21241 cttgggcggg gatgttaatt acctccatat cttacctcct gctacattct aaccgctatt
    21301 atatcatata atacaaggga ggcgcactga agagtttctc tagcgtgtgt ctttcaaact
    21361 ggctggctgc cggttagggc gataaaagca aatagattgt aatttgattg tctatttgat
    21421 aaactaaata agattactat ctcagtgcga tgagagggag acttatggta actgcatccg
    21481 ctcaaaactt aacaaatttt atacagcagc acggtgtctg gggtttagga gttgggatgt
    21541 ttctcgaaag tattggcatt ccttttgcct ctgcttttgt ggctttaact gctggcacaa
    21601 tcatcagtgc gggcaaagct tcttttctag aagtactgct gattaccact accggtttaa
    21661 ccttaggcag tatcgttagt tattttatcg gttactgggg tagttctgta ggtcgtttgc
    21721 tgttaaaaga cagaaaaaag aatgcggaaa aaagccgttt tctagagcac tatcggcgct
    21781 ggggtgaagt atccattcta tttgctcaat tatttggtac aacccgcacc tgggcgtcga
    21841 ttccagctgg tgccatgaag atgaaattgc aaagatttat tctctatact gccattggtg
    21901 gaacaattta ttgtacggca gctattggtt tttcttttgt cgttaccaaa ctgctagcaa
    21961 tgctgcagct gcaatattta gcattggtct tattatcctt agtcattgtt ttggctggtg
    22021 taatattttt ccggctgcgt gatcatcaag aaacgcatac taaaaaagag ccaaaaaccc
    22081 ctgcgaagta aaacgcaggg gtttttgcac taactagtaa agttgtttta aggcaatttt
    22141 gcatttttgc acacattgtt ccaattctgc tgcggcttgc gtaaaggagt ttttagcagc
    22201 aatatttgct gtttgtgaag aagctgtgcg taaatctgct gcagagcttt ggcatttttc
    22261 gatcgagttc tcaaccaagt tacggtagtc tgacatctta acacctcgct ttctagttgt
    22321 cctagcatag ttaagcaaga ctacagcggt aattgcgcat aataatccag caatggcggc
    22381 aggtgagggt caaactgttc cgcaaaggtc gcagccagct tattggtttc cagatagcga
    22441 ggaaggccat caggagggat gataaaatca acagaaagca tcacacgacc ctgctgaaaa
    22501 gctagttttt ctacaaacct ggggaattga tggatgctgt caactcgtcc gtcagtgatt
    22561 tctggtttgc ccacaaactc acctaaagct agtttccttt gcacctgcag ggcttgccag
    22621 ttacaatgat aaatgaacct ttgacaaaac cggccataag gccgggcagc agcaccgttt
    22681 gctaaaaagg gcattttatg cactacatga tcagcatctt gagcgtaagg atctgcggtg
    22741 atcacaaact cactacaaat tagtttatca tccttggcca aagctgccag cagggagggc
    22801 aagttttctt gcagcgcatc ttttagccag actgtataag gtgtgccgtt actattgttg
    22861 ccagcggagc caaaaggagt cttaacaatc cagacagctt gttcaccagc aggtaactgc
    22921 gctatactat gcttggtata ggtctgttgt agaggtaaat aacgtttacc gccattttcc
    22981 tccaacgcag cggctagata ggctttagag gcaaactttt ccggtaaata gccaaagatc
    23041 ttaatcccct tacttgctaa ttgagcaaat aagacatcat caaccgtaca aaactgggta
    23101 tcaagatagt cgtaagtcag ctcggctgtt aacaggcttt gtgggcttaa agatacggca
    23161 gcttgtctag aataacactc aactaggccg gctagacctt ttaagtgatc gccaaaaagg
    23221 taccaagagg gatacggggt attccctctc tggtacctta aggcacgcgc ccgcagggtt
    23281 tcctgcggac ttagctgccg tctgatttcc ggtaccgcta aagcagcggc taacatattg
    23341 tcatgaacgg cgatacataa agttggcaaa tttctcacct gtggtagcca cgtacaagcc
    23401 tttcccaata gcggcgtaat agtgactctt gattcggacc ggaaggttcg taaggcgggt
    23461 ccagagcctc catggcggac atatcttccg ctatttctac atcagggtca ttgattttcc
    23521 atggttcagg ataatatgac atctggttac acccccctag gtgttttatt cggtctgctt
    23581 ttagtatctg taaaattacg aaaatgttac cgataaaact ttggcaggac taccaattta
    23641 aaaaagtgat cttgaaaaac atctttaaac aatgcgtttc tattataaga agaatacaaa
    23701 aaaatataca aaactaaaaa gatcagattt gtcaaattga agtaaagcta atcagggagg
    23761 tttataaaaa tgcaattaac tgttttaggt tgttatggac cgtacccagc agcaggacaa
    23821 aactgctccg gctatctctt gcaagacgat ggtgtcaatg ttttattaga ttgtggtaat
    23881 ggcgtgctta gccggttacg ttatcattgc caaccaggtg agcttgatgc tgtgatgctc
    23941 agccatttac atagtgatca cagcagtgat gttatgatct tacggtatgc tcttcaaatt
    24001 aatgggagac agaatactga atctccactt aaggtttatg caccaccgca gccaaaagaa
    24061 gaatatgccc gtttagctta caaaaactat ctacaaagcc aaccgctatc tgctgattca
    24121 aaattaacta ttggcaagct gcaatttaca ttcagcaaag gtgtgcatgc ggtaccgagt
    24181 tatgtaataa ctgtgacagc agaaggcaaa aaattagttt atagtggtga tactgcctat
    24241 ttccctgctt tagttgaagc cgtgcaagga gctgatctct tcctgtgcga agcaaatttt
    24301 cttaaaagtg atcttcaatt aggtaaccct aaccatttgg ctgcttacca agcggcagaa
    24361 gtggccaagg ccggtggcgt gaagcagttg gtactgacac accatcaccc tgaacgggat
    24421 ccccaacaag cactagcaga agcacaagca gtttttgtaa atactacact ggctcgtgct
    24481 ggcagcttgt atcgtttgta gttgaggcta ttcttttgtt cgacctttgc ggtggcgaga
    24541 tactgccaac agctcagcca tggggttagt gggtccgtag ccgtttccta cagcgagacc
    24601 atgggcaata gcctgggtaa taaatcgttt ggctgtttgt actgcagcta atggttgttg
    24661 acctttagcc agttcggcgg tgatggctga agcgtatgta caaccgctgc catgagtgtg
    24721 tttagtgtgc agcttgggct cggcaaagcg atgaatagta tcgccatcat aaaaaatatc
    24781 gaccatttct tcccccggca aatgaccccc tttaactagt accgtacgac aaccaaagcg
    24841 atgaatttct ttcgcagcca tgatcatctc ttcaactgta cttatttcgc gacctaacaa
    24901 aacactggct tccggtaagt ttggtgtgac aaccaaagcg agaggtaata atttcttgca
    24961 caaagcctcc acagcagccg ctggtaaaag agggtcacca ctttcggcaa ccattacagg
    25021 atctacaacc aacttacgga cattgaattt aattagcatt tctgctacgg cagaaattaa
    25081 ttctgcatca ccgagcatcc cagtttttgc agcatcaata tgtatatcgg agagcacagc
    25141 ctccatttgg gcggcaacaa aagcggcagg aacagcatga ataccatgca caccaatagt
    25201 attttgcgca gtaagtgctg taatcgcact agtaccgtag acacctaggg cggcaaatgt
    25261 ttttaaatct gtttgaatgc cggcaccgcc gccagaatca gaaccggcga tcgtcagagc
    25321 tttgaacatc cttgctctac ctcctcttgt ggtcaagttt gacctcttaa ctttaacttc
    25381 atccgccact tagcagccct attgtacaca gagcttgtca atgttagaat aatacttgcc
    25441 aacctttaaa ggaaagggtg ctaaatttag tggagagaga gttatcatga atatacaaca
    25501 aattttagca caatttgaac ctatttgcac gcccagacta atgatgagga agattactag
    25561 agacgatgca acacaactat ataaactgac ttctagtccg gaaatgacaa aatacttaca
    25621 ctggtatcct agtcagagct tgtcagcagt taaagttgtg attgagcaga ggttaactga
    25681 atataaaaca ggagaaacaa gagctctagt attagctatt gctgatttac acaccaatca
    25741 attagtgggt aatattgctt ataaattaac tgatcgtgct ggtttgactt gtgaaattgg
    25801 ctattggctt ggcaaagagt actggcatca aggttatgcc agtgaggccc tcaaagcatg
    25861 tctacactat ggttttacag tattaggttt cgaacggatt caatgctgct gctccccaga
    25921 aaacattgcc tcttggcgtg tcatggagag agcagggatg caatgcgaag gaatcttgcg
    25981 caactcctat tatctgaaag gcaaattgta cgatgataaa gtctatgcgc ttttaaaagc
    26041 agaataccaa gcattgcagg cacaagctac agattaaacg gcagtttgaa tagttcattt
    26101 taaaaaagcg aaatcagcag ttctgtgcag gaactgctga tttttttatc ggagaggaaa
    26161 ttttatcaaa ctaaccatta attttggaat tgaacatcgc agaggctttt gtacagacca
    26221 gcttggcgca gcaaatcctc atgggtacct ttttcgacga tctccccttc tttgaggaca
    26281 agaatttgat cagcgcgttt gacagtggat aagcggtggg caatgacaat aattgtgcga
    26341 gtgccggcta gttcttgaat tgctgcttgg attttagctt ctgttgctac atctacggcg
    26401 gcagtggctt cgtctagaat gagaatgggt gtgttgcgca ggacagcacg cgcgatggaa
    26461 agtcgttgtt tttgtccacc agataacctg actccacgtt caccgatata tgtgtcatat
    26521 ccctccggca tttcctgaat aaagtcatcg gctttggcaa ttattgcagc ttctctaatt
    26581 tcttcaggtg tgcatgcttt aacaccgtag gcaatattct cggcaatgct tccgttaaat
    26641 aagaagacgt cctgcagtac aatactgatt tgattacgta aagaagccag cgtaagatct
    26701 tttaagttat gatcatccag actaatagta ccctccacag gttcatagaa gcgggcgagc
    26761 aaactaatga tggtggtctt gccaacgcca gtcggaccga ctaaggcaat catttcgccg
    26821 ggttgtgcgg taaaagttag atcttttagt acgggagtat ctgcttggta gtggaaagag
    26881 acattttcaa agctcacccg tcctgtgctt gtggtcaagg tcagggcatt tttgtgatcc
    26941 tgaatatctg gttcggtatc aagcacttca aatactcttt ctgcaccagc aataccttgt
    27001 tgtaaatctt ctgtgacgcg ggccagcgtg gcaatcggtt gatagaaaag tgataagtac
    27061 attaaaaagc ccacaatgtc tgccacagag agattgcctt gcagggctag atagccgccg
    27121 aagccgacca caatgactgt gccaaaagcg ctgactgctt ctacggcagg gtgaaagagg
    27181 gcacttaatt tcagggcatg cagaattgct tgagcatgag cggcggcttt ttcggcaact
    27241 ctcatttttt ctttacgctg ttggttaaat acttggatct cgcgcatgcc agaaaggtta
    27301 tcctgcagta cggcatttaa gtcggctacc gctacttggg cagtacggaa aaagggacgg
    27361 actcttttcg taaaaaaata gccgctataa agcaggatag ggactggaat tagggtaagc
    27421 agggccaagg tgggatggat aacaaaaaga atcatggtta ccccgagtag aattaaaaga
    27481 ttggcaatta agtctggcac agcatgcgca atcaggactt caaatgtcgc agtatcattg
    27541 gtggtacgtg acattagttg accggtttgt ttatcattgt agtaactcat ggataacttt
    27601 tgcagatgat tgtaaacaat gactcgcatc tcggctacca gtctccaggc cgctaaatga
    27661 ctaagataac gatagaaaaa agtgaagaga gcacgactga cataaaggac actaagtatc
    27721 agagcgagag tgcgaatttc gctcatagcg tggggtcgta cggtcagttc agttaggatc
    27781 ccagttaacc tcataatcag atatggtgct gccaggttga ggccagttat cgcccaacag
    27841 gctaactcct gtaataataa gcagccacca atagggtttg gcgtattgga ctaagcgcag
    27901 tacgtgtttc atatttcctc ctaggtaaaa agtgtatgct gtgagattaa gctgctgcgg
    27961 acagctctat tactattcgc aagcttactg aagattacct tcttttgata gcataaacag
    28021 agtaactgac tttataacta gcgacactga tgatttctat caattattcc tgccgctatc
    28081 atatatattt agagaactct taaaatcttc ttaccgctga catgagctag tcgtttatta
    28141 actattttaa cggctaattt tatgtaacaa agcattgatg tacaaatagg gttaagcgga
    28201 gggatactat gcgggttatt attgccggcg gcggcgagac cggaaaagcg ctggctgtcg
    28261 tgttatctgc ggtgcaggaa gtggtagtaa ttgaaagtaa ccgcagccgc gcggactggc
    28321 ttgcgcaaca tctagacggg caggtaatat ggggcgaagc acaatcttta cagactttat
    28381 tggctgccgg gactggtggt gccgacttat ttgtagcggc cactggtgtg gatgaaataa
    28441 atctgctcgc ctgtttgaca gcaaaaagac taggctgtat taaagtagcg gcttgcctga
    28501 agaatccaga ttactgggag ggtcagcatc ttttaaacac tgaactgaag tcgctagatg
    28561 tactattaca cccagtttta ttggccgcac atgcagttgt taagcagctt attaatccta
    28621 gttttgttgt caatttaagt ttcgctaatg gccgcgtggc tttattggcc ttagagctga
    28681 cacagaattc tcattttgta gggcagcaac tacagcagtt ggaattgccg gggcaggcag
    28741 tgatctgtgc tgttacccga cagggtgaaa cgagtgcgcg tgcgaaaaat tttactttgt
    28801 ctgcaggtga tcagatttat gtgatcggtg atgctttacg caaaagccat tggctgccct
    28861 caagtgagca aatttcgccg ccgcaaagag tattgattgg tgatggatca tggttagggc
    28921 agcaaatcgc ttctttaatg ggcagttctt tgcagcatat ggaaaatgtt ctcctggaag
    28981 ctgatctggt acggtgtgag gaactggcag atggtctgcc ccgttcacga atttatcaag
    29041 gagcaataac agagcatact ttcttagctg cgattgttaa cgccaaaaca gattggtttg
    29101 ttgccgccag tgataatgac ctggtgaata ttttgtcagc cctgctcgcc aaaaatttgg
    29161 gcgtgcgcgg catgatcgtg gtggcgcgca accagtccgc tcaggtagcg ctaaagacgg
    29221 cgggtattaa taatattatt tctcctaaaa cgctggtggc tgaaaaagta ttaagcttac
    29281 tacaacggga taattggcat aattttgcca ttatcggtga cggaccggtg caagccatag
    29341 aggttgtggt gcaggaggga atgcccttag tacataaacg ttttggtgaa gatcgtttag
    29401 ctgatggttt gatctggggt gccatggtac gccagggaca gcttatttca ccgcatactg
    29461 ctgagcgggt acaagccaat gatcatttaa tattactgat gccccaagga ttgagtacaa
    29521 ctagtagtgc tttgcttact gtggaaaatg ttattgtgaa aaagaaaacg tcgcccaggt
    29581 tgagccagtc atgaggtggc gcggtatttt ttttctgctg ggttctgttt tgctgtatct
    29641 agcttttgcg atgcttgttc cactactctg ggcaatgtgg caggcaagtg caacacgttt
    29701 ggactttagt atcacaatta ttttgacgat tactgtcgcc tggctgtttc tgcgactggg
    29761 acgaccgcct actgaactac gactgcagga tgcttttgct tttgtaactt taacctggtt
    29821 gtttgcttct ctctttagtg ctttgcctta ttatttttct ggttgtgtgc caactctaat
    29881 tgatgcatat tttgaggcta tgtctggttt ttcaactacc ggtgctacgg ttattatttc
    29941 tgtagagagt gtagatcaag ctatcctctt atggcgggct atgacacatt ggctgggtgg
    30001 catggggatt attgtgttat ttgtagctgt gtttccacgt ttaggagtca gtgccggctt
    30061 attgctgcag gcagagacac caggaccgat gacccaaact gtaacacccc gcgttgctaa
    30121 ttcagccaag attttatggt taatttatat cgctttaaca gcagcacaaa ctatcatctt
    30181 aaattcagtt ggttttactt tttttgaagc gcttacacat gcctttgcga ccatggctac
    30241 tggcggcttt tccactaaaa atgccggtgt ggctagcttt gacaaccctg ccgctgaatg
    30301 ggtaatcatc gtgtttatgc tgctagcagg tgtaaatttc gccctttatt atcatttgtt
    30361 gctgggaaac atccgacaag tgctcaaaga tgaagaatta cgtttttatt taattgtggt
    30421 aattgtggcc acgctgttgt tgtttatttc cctgctgcct ttaccacatg ctccactagc
    30481 tgctctgcgt cacgccttgt tccaagttag ttcagtggtt accactactg gatttgccaa
    30541 tgtagatttc gatctgtggc cggaattttc tcggatgctc ctcttactgt taatgtttgt
    30601 tggtggctgc ggtggttcta ccggtggtgg actgaagcag attagaattt tagttttggg
    30661 taaatatttg ctgcgtgaat tacgaaaggc cattcgacct tgggaggtag ccccgctgcg
    30721 gatcggagac caagttatct ctcaacagtt gatcaatagg attattgctt ttgtctgctt
    30781 atatctactc attttttcct ctgctgttct ttacctgaca tctttaggtt gtgattttac
    30841 cacatctcta tcagcagcag cgtctgcact aggcaatata ggtcccggcc tggccgcagt
    30901 cggccccatg catacctatg ctaaattaac tgctggtgct aaaatatgtt taacattgtt
    30961 aatgctcatt ggacgtttag aaatctatac tgtattatcc tttttgctgc ccgaatcatg
    31021 gtaccggcat aaagcctcat ggcgacagct taagtagcat aacttcactt tagcagtaca
    31081 tactagaaga gaataaagga ggattcccat gccaaagtat aagaaaaaac cggaaaaaat
    31141 gacgctgcgt gaactagaga tggaaaaaat gaaaatggaa attgctgaag aattaggttt
    31201 gagtgataaa gtaaaagaag ttgggtggga aaacctaacc gctcgagaaa caggcaaagt
    31261 aggtggcgta atgtcacgcc gtctacgcaa agaaggaaaa aataaataaa agtactaaaa
    31321 gctcctgcag gagctttttt gattgccgtt tctcgccgct gcgctcgtac ttgtatttac
    31381 tcttttacgt ttgatatgat ataaatgaag aaaagcatta ttacgtagga ggtacatatg
    31441 cgtctcggtg cacatctatc tattgctaaa ggttttgata aaacagccca tactgccctg
    31501 gaaataggtg ctaacacttt tcaatttttt acgcgcaacc cgcggggggg taaagctcgg
    31561 accatctcag aacaagaaat tactgcctgg aaagagttac gccgaacata tgatattttt
    31621 cagattgttg gtcacctgcc atatacagta aacatggcgg ctaaagaaga tcgtccgtat
    31681 aactttgcca aaatggtgat tacagaggat ttacaacgta tggaagccgc aggagtagaa
    31741 tatttagtga ttcatcctgg ttcacataca ggagctggtc ccgaaaaggg taccgaacgc
    31801 attgttgcct gtttggcaga agccttttta cctcatcatg gccagacgat gttactttta
    31861 gaaacgatgg cgggacaggg atcggaaatt ggcaccttag cagaaattgc tgagattatg
    31921 gagaagctga actttccgga gcgactgggt atttgtcttg atagctgcca tctaacaggt
    31981 gccggctatg atttccggca aaaagaggaa gtaaagcgtt tagttactga tttagaaaat
    32041 acagtaggga tcagcagagt taaagcggta catttaaacg attctaaatt tgaacctggt
    32101 accaataaag atcgccatgc gcgccttggt gaaggttatt taggcaggga gggcattatc
    32161 aattttgtta ctcaccctgt gattaaaaaa ttacccatag ttttagagac ccctgttgat
    32221 gattacaaag aatatggtga agaaatagct cttttacgca gctggcttgc ggaaatagca
    32281 taaaaagata atttactggc caaggcacaa aaaggacgga gtcagatgct gactccgtcc
    32341 tttggcttat atttatttaa cttttaacat actccaagat ggacttccag ttacagtatt
    32401 cgctgatgtt gtcttgggca atttgtactg catgcgtatc gctgggatga atgcgtcgtg
    32461 atacttcatg caagcgctcg atcgccgtgt aggtgtcata atgaaccaga atgacaggta
    32521 cttttttctc agtggctttg gaaataacac taacatcggg atagagcccg cctgtaagga
    32581 ttaagacaga agtgctggtt tccaaagcgg tgatggcgat atcggaacga tctccgccgg
    32641 taattactgc tttgttcggc gcacgccgca gataacttaa ggcaccctct atggtcatgg
    32701 agccgattac tacttcttct actaggcgca tcattttgct ctcatcacca gccagcagtt
    32761 ctccgcctaa actatcatag tactcacgta ccgttggcgc agcaatctca gctttttggg
    32821 gaataacgcc gaagacaggg tagcctaact tttctagaat cggcgtatag acacctttag
    32881 ttttatctaa aagggtgcgg ctaacattgt taaagatatt gcctaacatt ggcagtccgg
    32941 caagacgaaa atgttcatta tggaaaacag ttttatcaaa gctataatca tctgttaagc
    33001 gcacaacata aaccatggcg ctgctgagca ttttagctaa gctgattgca tctaaaccta
    33061 cactagctgc cgtataggga gagagcgaac catcaacaat cactgtatct acaccaactg
    33121 atacctgttc ataggcctcc atgatttttt tccggtaagt ggaggtctcc agattcgaac
    33181 cccacgacaa ttggaagggg cccgtgtgga caggagagag tacagattca tcatggggca
    33241 gatttaaaac ctgttttagt aaacggacat cgtcatcgct tttattctgc gaccccctgg
    33301 ttggatcaag tggcttaaag taacctactt taattccttc ctcacgcaat ttaagcgcca
    33361 agcctaaagc aattgcagtt ttaccactgc cggccatacc gctaataaat agacttttca
    33421 tttacttcac cctttctact ccagattgtc gccatacgaa acagtaattt tcacatccag
    33481 tgctacggca ctgtcaggat atgcaattac cggatttaaa tcaatttccg ttatttcagg
    33541 gaaatccaaa gaaagttttg cgatgcgagc aatagtttgt accagtgact cgatgtcaga
    33601 gggggtgctg ccacggtaac cccgtaacaa agtataagct ttggtttccg taatcatttc
    33661 ctcaatatcg tgcagggtta gactttgggc taatctaaag gaaacatctt tgagaaggtt
    33721 aacgtaaata ccgcccaaac caaaggcaat taaaggacca aactgtaaat cgcggctcat
    33781 gcccacaatt aattcattgc cagggggcat cattttttga atttcaatgc cataaatagg
    33841 tgtacctggc attaaacgac taacactctc catgatggct acataggcat ccttgacttc
    33901 ctgtgctgat tctaatccta cttttacgcc acccacatcg cttttatgta taattttggg
    33961 ggaggcgatt tttagtacta ccggataacc catgctttcg gctttctcta tagcttcatc
    34021 aggactgttt acgagataaa caggagcaac cgagataccg tacgcttgtg ccacttgtgt
    34081 tgcctcgtta cccaataaga ctaggcggtg gtcacgcagc acatcgtaca aggtggcctt
    34141 cacagcttgg tggtcaatat ctgtgaaatt gtaaagaggt ttggcagatt caattacttg
    34201 acgcatgtta gcgtagccca ccataccaga aatagctttc agtgccggtt ctgggaatgt
    34261 atagctgggt atgccagagt gggttaggaa gcggctgcct tcggcaagtc cttcgccccc
    34321 cataaaggct gcaaatagcg gcttctgagg atatttttgc ctcatttcta ccattaattc
    34381 agctgttttc agcggttcgg taacatcggc aggtgaaagt aagactaaag cactttgtgt
    34441 gctctcatca tccaacaccg cttccagtgc tacacggtaa cggtcagcgt ttgcatcacc
    34501 cagcacatcc actgggttat aaatgctagc ttctgctggc agtagttccc gcattttagc
    34561 agtggtttct ttactgaaac gagccatctc cagtttattg cgttccacgc tatcagttgc
    34621 aataatacca ggtccaccgg aattggtaac aatggcaatt tgcttacctg ccggcacagg
    34681 ctgattgaca aaagcaatgg ccaaatcaaa taagtctgcc atgttatcag cccgcagcac
    34741 accgcactgg cggaaagcag tatcgtaggc aatattactg ccagccagag caccggtatg
    34801 agaggaagcc gcgcgggcac cagcagagga agtgcctgct tttaaaatga taatgggttt
    34861 acgtttactt gcttcacgta cgacttccag aaaacgctgc ccatcagaga catcctctaa
    34921 atagcagagg ataacacttg tttgcgggtc ttcacaagca gaccagatga agtctacttc
    34981 atttagatca gctttgttgc ccatagagat aaagcgggaa aaacctaagc ctacgctgcg
    35041 gctccagtct aaaatagcca gcagcatggc accactttgg gagataaaag caattttccc
    35101 ttgtggtgga gaaccgggag taaaagaggc attaaagggg gtatgattgt ccatgattcc
    35161 cactacattg ggaccaacta gacgcatctt atactttttg caaatcttta caagctcatg
    35221 ctcccgtttt aaaccttcct caccagtttc cttaaaacct gccgtcacaa caactaaaaa
    35281 ctttacacct gcttcaccgc attcttgcac cacgttaatt gatcgttcgg cggggacagc
    35341 aataacagca acatcaacag aatccggcac ttttttaatt gaagggtaac atttgttgcc
    35401 aaaaatctct gtttctttag gattaatggg gtaaacatga ccggtatagc cgctttcctg
    35461 taaatttgcc aggatggaat taccgatttt cccttctttc ttggaagcac caactacagc
    35521 aatagaactt ggattaaaaa gaatacttaa atcgctcatc agatcactca ccttctgacg
    35581 tggcttaata gtgaaaaaaa taacaaattg ctcatctttg attgtaccag tattcagctc
    35641 aactttcaat gcaataatgg tgaagctaac aacatagtaa aattttcgat aaatggcaag
    35701 attctgtaat tactatattt caccgggtaa gcgcccctaa ccatgatttg ggaaaccact
    35761 gcagcaaatt acagttttag tctgaagtag aagcaacgaa aatatcccca catttctacg
    35821 ttataggtaa ggagctgcac agcaataaca tatttctgtc gattagatca caagtgagaa
    35881 ggaaagtaat ggaattagtc gaaatactta agaaaggcag catcagataa agtctgtgtc
    35941 tgccagtaca ccttgcttga cctaattgcg aagcgaagtg tcatagttta gccagtatta
    36001 aacaatcagg gtggtgggta gctttgcaag attataacca gctcgaacaa gtctctacta
    36061 agctgctcgc agccgtagtt gagggcagaa aacagattat aaccttagta aagcaggcgc
    36121 gtgctgagaa atgtgtgatg atggctgagc tggtagagtt aaaagcagat atactgaaag
    36181 taattaccca agcagatcgt ctggaacaag actataaatt agcacgcaaa catgtgctgg
    36241 cggtcaacgg taattttacc aagtttactg accaagaaat acaaatagcg tatgaagagg
    36301 ctcaacacct gcaaaatcag ttaatcaaga tgcatgaagg tgaggttagc aatcagcagc
    36361 agcaaaagga gctggaggca aatttactgg agctgaatga gaagctaaat aaggcggaaa
    36421 agctggaagg ccagacagac ttggccctgc aattactggg cggtgagata cagaacacta
    36481 atgctaaatt agacgaatta caattgcgac agcaattaag cgtacgcatc attaaagctc
    36541 aggaagagga acgcttgcgt gtcgcaagag aaattcacga tggccccgcc caatccatgg
    36601 ctaatgttgt gctgcgagcg gaaatttgcg agaagttaat ggtgctggag ccagaaaagg
    36661 tcaaggaaga gctgcatgat ttaaaagcca tggtcaaaga aagcttgcaa gaagtacgta
    36721 aaattatctt taatctccgc cctatggttt tagatgattt aggcatcgtg cccaccttac
    36781 gtcgctttat tagcgagctg caaaaacatg ccaaactaac aattgatttg gtggttttgg
    36841 gcggcgaaga aagtcgtttg cctaaaaccc tggaagttgc tatttttcgc attgtacagg
    36901 aagcacttaa caatatcaac aagcatgcgc aagcgcagcg ctgtattata aagctggaaa
    36961 tattgccaaa tcgtctaaac atttcagtta ttgatgatgg acgtggtttt gacacccaac
    37021 tttttaccac taccaatacg gaaagctttg gcctcatagg gatgcgggag cgagtggaac
    37081 tattaagggg cgagatggaa attacatctg ctccaggcag tggtaccgag attcagatta
    37141 taattcccat caagaggtga agcagatgca gaaaacaaca attttaattg ctgatgacca
    37201 ccctcttatt cgtgatgggt taaagcgcat tttagagttc catgaagagt ttaccgtcgt
    37261 gggggaggct gccaacggcg aagtggcctg tacgatcgcc cgtcaatgcc agccagaagt
    37321 tgttttaatg gatataaata tgccgcagat gaatggcatt gaagccacca aacaaattaa
    37381 acatgattgc gccgctacaa atgtcattgc tctcaccatc cacgatgacg atgaatatat
    37441 ttttgagtta attaaagctg gcgtctctgc ttatgtcctc aaagatattg aagccgactc
    37501 cctgatcaat acgattcggg ccgtggccag cggtgaaacg gtctttgatg ccacgattac
    37561 cgcgaaaatg tttacggagt ttcagcgaca aactcgccaa aacagtctgg acgaaccttt
    37621 aacctcacgg gaagcagaag tgcttacgct cattgctcgg ggcaggaata ataaagagat
    37681 cagttcagaa ctctatatta gtcaaaaaac tgtaaaaaac catatcacta atatcttccg
    37741 gaaaatcaat gtggcagacc gcactcaagc tgccctttat gccgtaaaaa accgcatcgt
    37801 caaactttaa cctagaacct cgcggggggt tctttttgtt tgggtaagtt aaagtgtagt
    37861 taatccaccc ccaatcttgt cagctattag agaaaatagg actatagtcc tgtcataatt
    37921 aagactttgg cttgatggca acttgtggaa gtaatgttaa actggtgaca gcagaaagct
    37981 ttcggagaaa taattatata tatatttagg aggtaagaag ataatgaaag aaatggtaaa
    38041 aagatttatc agtgaagaat ctggacaagg aatgaccgaa tacgcgctga ttttagctct
    38101 tgtttctatt gctgctattg ctgcattaaa ggtgctaggt cccaaaataa cacaagtatt
    38161 taaagaggtt aatgaagagt taccaaccgt tccagagtcg taaaaattga actaatacta
    38221 gaggatttag ccctgcccaa cgagggcagg gctaaattct gctttggaga ctaaagaagt
    38281 gggtgaaaga tgatgggaaa atggtttaaa aatcaaaaag gacaggccat tgtggaatca
    38341 gccattgtgc tgcccatcat tttgctgctg ctcttgggga tggtggaagt cggccgtgtc
    38401 tgtaatgcat atctggtagt gacgcaggct gcccgccatg gggctcgcta tggggcagtg
    38461 ggagcaaatg atgtggaaat agctgaccgt attgccagcg tagttacccc cctgaatccg
    38521 acagacttaa ccattaccat cactccagca ggagcacgcc acacaggtga ggacttacaa
    38581 atccaagtga gctacccggt gcagttaatc acgcccttag ctggcagagt ggttagtaat
    38641 ccttggcttg tcaaaagcgc catcaccatg cgtgtggaat gaggtgagag gcatgcgctt
    38701 taatagtttt aaaagatatt tgcagaatga acgtggtacc gccatcgtga tcttggctgg
    38761 ggcgatgacg ctgttgatgg cgtgcaccgc tttagtagca gacattggcg ttaattttgt
    38821 tacccagtcc cgtctggcgg tggcagctga tgctgctgcc ctggctggag caactaaatt
    38881 taaggatggg gcagcgcaag tgcaggaaac ggcactggcc atagccgcgc aaaatggtat
    38941 caaggctgaa gacgtgattg tcaatgtagc tggcagtggg aaagaagtga ccgtcacagc
    39001 gaccgcacct gtacaattgt tttttgccag atcatttagt aagcaagagg gagatatggc
    39061 gcaaagtgcc cgagtgatcg ctgggcgccc cacagccatg accaatattg tccctttggg
    39121 tattaatgaa aatgctaatt ttgagt
//
LOCUS       DASRMS010000189         1171 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_789775, whole genome shotgun sequence.
ACCESSION   DASRMS010000189 DASRMS010000000
VERSION     DASRMS010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789775"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..327)
                     /locus_tag="VFC74_09120"
     CDS             complement(<1..327)
                     /locus_tag="VFC74_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25528.1"
                     /translation="MKNVTLTQIRKSVEAAGGTYKKCGFTLNGNNAYEVNSKVYTKAQ
                     LVDAYHAGDLWQRRKPECEESQMTPNLEKHTERVALLVTPSQKKFLQDNVGDRGINTF
                     LRWLLDN"
     gene            complement(748..>1171)
                     /gene="ssb"
                     /locus_tag="VFC74_09125"
     CDS             complement(748..>1171)
                     /gene="ssb"
                     /locus_tag="VFC74_09125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK25529.1"
                     /translation="NGNAVCNFSLATTEKWKDKTTGQPVEKTEWHRVSIFGKLAEIAG
                     QYLRKGSKIYIEGRNQTREWEKDGVKRYTTEVIVDMRGAMQMLGGKQDGQQQSQAPAQ
                     QQQYQPPAQQQQLVRPPNDSHSATNPSPIDNFDDDIPF"
BASE COUNT          248 a          287 c          253 g          383 t
ORIGIN      
        1 gttgtctagc agccacctta aaaacgtgtt tatacctcta tctcctacat tatcctgcag
       61 gaatttcttt tgagatggcg tgaccagtaa ggccacgcgc tctgtgtgtt tttcaagatt
      121 tggcgtcatc tggctctcct cgcattcagg tttgcgccgc tgccaaagat caccagcgtg
      181 gtacgcatca accaactgcg ccttggtgta aaccttactg tttacttcgt atgcattgtt
      241 accgtttaga gtaaaaccgc actttttata agtaccgccg gccgcttcta ctgatttgcg
      301 aatctgggtc agggttacgt ttttcatggt gacttccttt cgttagtgtt tcgttttgct
      361 gtttatgtgt cctattatac gcatacacat acacatagca acaccttttc cgtttatttt
      421 tatttatttt gttttgcgcc tttattgcgc ggcggctcgc tttttaacgc cttatttatc
      481 ttgtccatgc tatccagcgg atcagtgacg aaaacaccgt cacggctgac tggtgttgtt
      541 aggttttgtc tatgaactcg acaacatctt cacgcgatag aaacttatgc ataacacctt
      601 tatacgtcac aaaataaaca tcatgaggga gctgtgacag gtacacagtg aactcacctc
      661 gccgccatgt gttgaagcct gtttttgccc actgcatatc tgtcatccat aaaaaaaccc
      721 cgcataacgg ggctgtgttg tgctagatca gaaaggaata tcgtcatcga agttatcaat
      781 cggtgacgga ttggttgcgg agtgactatc gtttggcggc ctgaccaact gctgctgttg
      841 cgctggcggc tgatattgct gttgctgtgc cggagcttgg ctttgttgct ggccatcttg
      901 cttgccacct agcatttgca tagcgccgcg catatctacg ataacctcag tagtgtagcg
      961 cttcactccg tccttttccc attcgcgggt ttggtttcga ccctcgatat aaatctttga
     1021 tcctttgcgt aaatactgac ctgcaatctc tgctagtttc ccgaaaattg aaacacgatg
     1081 ccactctgtt ttctcgactg gctggccagt tgttttgtct ttccattttt cggtggtagc
     1141 taaactgaaa ttacagaccg cattgccgtt a
//
LOCUS       DASRMS010000190         4854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_591169, whole genome shotgun sequence.
ACCESSION   DASRMS010000190 DASRMS010000000
VERSION     DASRMS010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4854
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_591169"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            17..1069
                     /locus_tag="VFC74_09130"
     CDS             17..1069
                     /locus_tag="VFC74_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003514906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HZK25530.1"
                     /translation="MTIPLGDFLQQLVSNPALLVIATLTAAVILVNGWTDAPNAIATC
                     ISTRAIGPQKAILMAAIFNFFGVLVMTLVNATVAHTIYNMVDFGGNSHNSLVALSAAL
                     FAIVLWATAAWAFGIPTSESHALIAGISGAAIALQGGFSGINGAEWIKVIYGLFISTI
                     LGFCLGYLVVKLIEAIFKSTDKRQTRAFFVNAQVAAGAAMAFMHGAQDGQKFMGVFML
                     GIFLASGQGTVTSFTIPLWLMVLCSFLMALGTSIGGYRIIKTVGMNMVKLETYQGFAA
                     DLTGAISLLISSILGLPVSTTHTKTTAIMGVGAAKRLSCVNWSIVKEMVAAWILTFPG
                     CGLVGYLMAHLFLKIF"
     gene            1097..1729
                     /locus_tag="VFC74_09135"
     CDS             1097..1729
                     /locus_tag="VFC74_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017415167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HZK25531.1"
                     /translation="MVLKSDTKNFSYFAGFVQLAHFSLEAAEILNDTLQEFTVAKLKQ
                     QLAKIHEVEHTADVGRHELLNRLAKEFLPPIEREDIIRLAEEIDDVTDAIENVLINIS
                     IFNVQTIPTKINEFTEVIKDCCNSMIAALKEFENYKKSQTLHDEIVEVNRLEEVGDVL
                     YRQSIQKLLQFNKDPFALVVWKEILVSLENCCNACEIVANDIENITLINS"
     gene            complement(1776..2861)
                     /gene="ytvI"
                     /locus_tag="VFC74_09140"
     CDS             complement(1776..2861)
                     /gene="ytvI"
                     /locus_tag="VFC74_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation integral membrane protein YtvI"
                     /protein_id="HZK25532.1"
                     /translation="MKAIPVPLANILKLLAVIAGTCGGLYLAVVFFSYVAPFVIAFLL
                     ALGINPLVCWLAKKRKFTIPRSLAALITTTITLLVIFWLLLYLGSLLIKQATEIIKLL
                     PHLAPNLQHNLSVFVGGLGEQMSILPDYVLQGIQNFIVNLGTTISSYTAGAAFFVFDT
                     AKLLPFLFLFVLLTVLATYFFTKDYPTIRAGVINNVPHTWLKQYRIIKADMLAALLGY
                     LKATVILSFVTFILLLVGLLLLKVKYAFILALIISLFDALPAIGAGLFFIPWSLYLLI
                     ISEQQLAIELFILYVIVLGVRQLISPKILGDQFGIHPLSTMIAMYVGLKLIGAVGLLI
                     GPVIFLIVKSVVGYYTQGKSFKELIFD"
     gene            complement(3040..>4854)
                     /gene="ftsH"
                     /locus_tag="VFC74_09145"
     CDS             complement(3040..>4854)
                     /gene="ftsH"
                     /locus_tag="VFC74_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006716974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZK25533.1"
                     /translation="EPQGPNKNLVYFYLVGMIVVMLLNAFVFPALLKPKITEVDYGTF
                     LKKVEQGQVQTVEINETETEIAFKTKNDSGTEEIFVTGYIDNPELVNQLAKANVEFSK
                     VIPKENSPLLNIILTWIFPVLLFWGLGQLLMNSMQKKMGGNAMSFGKSNAKVYAEAQT
                     GITFADVAGQDEAKEALTEIVDFLHNPTKYKAIGAIMPKGALLVGPPGTGKTLLARAV
                     AGESKVPFFSISGSEFVEMFVGMGAARVRDLFKQAQEKAPCIVFIDEIDTIGKKRDNG
                     GFSGNDEREQTLNQLLTEMDGFSGEKGVVILAATNRPDSLDKALLRPGRFDRRIPVEL
                     PDLKGREAILEVHAKEVKMVREIDFNAIARATPGASGADLANIINEAALRAVKCGRQQ
                     VNQSDLEEAVEVVIAGYQRKGAVISPKEKQIIAYHEIGHAIVAAKQTESAPVHKITII
                     PRTSGALGYTMQLAENENVLLTKDEAFNRIATYTGGRAAEELVFSTSTSGAANDIEQA
                     TKMARAMVTRLGMSQNFDMMALETEANRYLGGDTTLACSAETAAQVDAEVLAIIKKAH
                     EKAISILKESMDKLHELAAFLVEKETITGDEFMSLLTS"
BASE COUNT         1394 a         1076 c         1009 g         1375 t
ORIGIN      
        1 aaacttggag gaaaatatga ctattccact gggagatttt ctgcagcaat tggtctctaa
       61 tccggcttta ttggttatcg ccaccctgac agcagcagtt atcttggtaa atggctggac
      121 agatgcacca aatgccattg ctacctgcat ttccacacgt gctatcggtc cgcaaaaagc
      181 tatcttgatg gctgctatct ttaacttctt tggtgttttg gtcatgactc tggttaatgc
      241 cactgtagcc cacaccatct ataatatggt agactttggc ggtaatagtc ataactcact
      301 agtggcgctt tctgcggcgc tgtttgccat cgttctctgg gcaacagccg cctgggcctt
      361 tggcataccc accagtgaaa gccacgcttt aatcgcaggt atttccggtg cagccatcgc
      421 tctgcaaggt ggattttccg gcattaatgg tgcagaatgg attaaagtta tttatggttt
      481 gtttatttcc acaattctgg gtttttgtct gggctactta gtcgtaaagt taatcgaagc
      541 aatctttaaa tccactgaca aacgtcaaac aagagccttt tttgtcaatg cacaggttgc
      601 ggcgggagca gccatggctt ttatgcacgg ggcgcaagat gggcaaaaat tcatgggagt
      661 ttttatgctc ggtatttttc ttgcctctgg gcaggggacc gtcaccagtt ttaccatccc
      721 tttgtggtta atggtgcttt gctctttctt aatggcctta ggaacatcga ttggcggtta
      781 ccgtattatt aaaactgtag gcatgaatat ggtgaaatta gaaacctacc agggctttgc
      841 cgcagactta acaggcgcca ttagtttgtt aatctcctct atcttaggac tacctgttag
      901 tacaactcat accaaaacaa cagccatcat gggggtcggc gccgccaaga gactatcttg
      961 tgtcaactgg agtatcgtta aagaaatggt tgctgcctgg atattaacct ttccaggctg
     1021 tggtctcgtt ggctatctca tggctcactt gtttttaaaa atattttaat tctgcaggaa
     1081 cgaaagaagg ggaaaaatgg tacttaaaag tgacactaaa aacttttcat actttgctgg
     1141 ttttgttcag ttggctcact tctcattaga ggcagcagaa attttaaacg atacgctgca
     1201 ggagtttact gtcgctaaat taaaacagca gttagctaaa atacatgaag tagaacatac
     1261 agctgacgtg ggcagacacg aattacttaa tcgattagca aaagaatttc ttccccccat
     1321 tgaaagagag gatattatcc gactagctga ggaaattgat gatgtcacag atgccatcga
     1381 aaatgtctta attaacatca gtatttttaa tgtgcaaacc ataccaacta aaattaatga
     1441 atttaccgaa gtaattaagg actgttgtaa ctcgatgatc gccgctttaa aagaatttga
     1501 aaactataaa aaatcgcaaa cgctgcatga tgaaatagtc gaggttaacc gtctagaaga
     1561 agtaggggac gtcttatata ggcaaagcat ccagaaactg ctgcaattta ataaggatcc
     1621 ctttgcctta gtggtctgga aagaaatctt ggttagcctg gagaattgct gcaatgcctg
     1681 tgaaattgtg gccaatgaca tagaaaatat cacgctgatt aattcataag actttacgca
     1741 aagaacccca gagagagcgc tctctggggt tctttttagt caaaaataag ttccttaaaa
     1801 cttttacctt gtgtgtagta gccaactaca cttttgacaa ttaaaaagat gacgggacca
     1861 atgagcagac cgacggcacc aattagttta agacctacat acatggcaat catcgtggaa
     1921 agtggatgga tgccaaattg atcgcccaag attttagggc tgattagctg cctgacaccc
     1981 agcacaataa cgtacaagat aaataattca atggctaatt gttgttcact aataatgaga
     2041 agatatagtg accaggggat aaaaaacaga ccagcaccga tcgccggcag cgcatcaaat
     2101 aaggaaataa tgagagcaag aataaaagca tatttaactt ttagcaggag caagcccact
     2161 agcaagagga taaaagtcac gaaactgaga atcactgtgg ctttaaggta accaagtaaa
     2221 gcggcaagca tgtctgcttt aataatgcga tattgtttta gccaagtatg gggaacgttg
     2281 ttaatgacgc cagcacgtat ggtaggataa tctttagtaa aaaagtatgt tgctaacact
     2341 gttaacagta caaacaggaa aaggaaaggt aatagtttcg ctgtgtcgaa cacaaaaaac
     2401 gccgcccctg ctgtgtatga agaaatagtc gtacctagat taacaatgaa attttgtatg
     2461 ccctgcaaaa catagtcggg taaaatactc atttgttcac caaggccgcc cacaaaaaca
     2521 cttaagttgt gctgtaagtt tggtgccaga tggggcagta gtttaataat ttctgttgct
     2581 tgcttaatta gcaacgagcc cagataaagc agcagccaga agataaccag cagcgtaatt
     2641 gtggtagtaa ttagcgctgc tagcgagcgg ggaatagtga acttccgttt tttagcaagc
     2701 cagcacacca acggattaat gcccagggct agtaaaaatg caattacaaa aggtgcaaca
     2761 tagctaaaaa acaccacggc caaatacaac ccgccgcaag ttccggcaat gacagcaagt
     2821 aattttaaga tgtttgccag aggcacaggt attgctttca atttatcacc cctaatttac
     2881 aattagtact gagccttgtt aagcatgtgc ttaactgctt agttacatta tcttcgtttt
     2941 tgacttgctt ggcaagcacg atctatgcac agcgtttcag ttcagcttca caatacaaca
     3001 aaaagcgggg tagctaccac cccgctctct tcctctccct taacttgtta aaaggctcat
     3061 aaattcatcg ccggtgattg tttctttttc tactagaaac gccgctagct catgcaattt
     3121 atccatactc tcttttagaa tagaaattgc tttttcatgt gccttcttaa taatcgccaa
     3181 tacttctgca tctacttggg ccgccgtctc cgctgaacag gcaagagttg tatcgccgcc
     3241 caaatatcta tttgcttctg tttccaaagc catcatatca aagttttggc tcatccctaa
     3301 acgcgtgacc atggcacgag ccattttagt agcctgctcg atatcattag ccgcaccgga
     3361 agtagaggtg ctaaagacaa gttcttcggc agcgcgccca ccggtatagg tagcaatcct
     3421 gttaaaggct tcgtctttag tgagcagaac attctcattt tcggccagtt gcatcgtata
     3481 gccaagtgct cctgaagtgc ggggaatgat agtaatctta tgcacaggtg ctgattcagt
     3541 ttgtttggca gcaacaatgg cgtgaccgat ttcatgataa gcgatgatct gcttttcttt
     3601 aggcgaaata acagctcctt tgcgctggta accggcaata actacctcta ctgcttcctc
     3661 taaatcgctt tggttaacct gctgacgtcc acattttact gcccgcaggg cagcttcatt
     3721 aataatattg gcaagatctg cccccgacgc cccgggagtc gctctggcaa tggcgttaaa
     3781 atcaatttcc ctgaccatct ttacttcttt ggcatgcacc tctagaatag cttcccgtcc
     3841 cttgagatca ggcagctcca cagggatacg acggtcaaaa cggcccgggc gtaataacgc
     3901 cttatctaaa gaatcggggc ggttggtagc ggccagaatg accacacctt tttcaccact
     3961 aaaaccatcc atttccgtta gtaactgatt caaggtctgc tccctttcat cattaccact
     4021 gaagccgccg ttatctcgct ttttaccgat ggtatcgatc tcatcaataa atactataca
     4081 gggagctttc tcttgagcct gcttaaataa atctcgcacc ctggctgcac ccatgccgac
     4141 aaacatttcc acaaattcag agcctgagat ggagaaaaac gggacttttg attctcccgc
     4201 taccgctctg gccagcagtg ttttaccggt gccaggaggt ccgacaagta aagcgccttt
     4261 gggcataatc gcaccaatgg ctttatactt ggtgggatta tgcagaaaat ctacaatttc
     4321 cgttaatgct tcttttgctt catcctgacc ggccacatca gcaaaagtaa tcccggtctg
     4381 tgcttccgcg taaactttgg cattactttt accaaaagac atcgcattgc cgcccatttt
     4441 tttctgcatt gaattcatca gcagctgccc cagcccccaa aaaagcagca cagggaaaat
     4501 ccaggtaaga ataatattta aaagcggcga attctcctta ggaattactt tggaaaattc
     4561 gacattcgct ttcgctaact gattaaccag ttctggatta tcaatgtagc ctgtaacaaa
     4621 aatctcttct gtaccggaat cgtttttggt cttaaaggca atttctgtct cagtttcatt
     4681 aatttctaca gtttgcactt gcccttgttc taccttcttt aagaaggtac cataatctac
     4741 ttctgtaatc ttcggtttta acaaggcagg aaaaacaaag gcgttaagca gcattaccac
     4801 aatcatccct actaaataaa agtaaacaag atttttattt ggtccctgtg gttc
//
LOCUS       DASRMS010000191         1980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_254793, whole genome shotgun sequence.
ACCESSION   DASRMS010000191 DASRMS010000000
VERSION     DASRMS010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1980
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254793"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            324..1607
                     /locus_tag="VFC74_09150"
     CDS             324..1607
                     /locus_tag="VFC74_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014794461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HZK25534.1"
                     /translation="MKYTQNKKILQVTEKTLIVGVDIASETHYARAFDYRGIEVGKLL
                     KFSNDAEGFGQFIAWVGSIEQKQCKNKVMIGMEPTGHYWFNIAQQLKDHNLKLVLVNP
                     FHVKRSKELDDNNPTKNDRKDPKTIAMLVKDGRYMEPYIPEGIYSELRIAMETRWQIV
                     KQLNRIRNRMKRWLSIYFPEFNQVFANWEGKAALIVLQDFPTPTRILERGVEGIVGRW
                     KQDKLRGIGRKRAMRLIEAAKQSVGVREGLMAAENDLLNMLENYKLQMQHYEKTMNMV
                     EQLVKQIPGLAEIVKIKGVGLVTGAGFVAEVGDISRFEHPKQIQKLAGLNIKENSSGK
                     HKGKSSISKRGRRRLRSLLFQVAMPLVANNKEFQELHSYYTTRNQNSLKKKQSLVLLS
                     CKLIRIFFALLTKDIAYDPEKMMSDIKRPLMQDAA"
BASE COUNT          677 a          377 c          484 g          442 t
ORIGIN      
        1 tatttggatg ttgcggctga agcatttaat gacattgtaa taaccctcgg tccatgtgta
       61 aatgaaggtg ataaaataat tgttgaatcc ttaaccgcca aatattgtcc gacggcaatt
      121 gttagaaata gtgtacttga aaatagtata agattataaa ttacctcagg gcaaggaata
      181 agcatatatg gtaggaattt ccccctattg gaagcattcc tgtaataaac aaaaatgaac
      241 tttgaaaaaa agagtacctc ctgttaacat acgaggtgtc gacttcgcga agcgaccctt
      301 aaataacaga ggaggtactc aagatgaagt atacccagaa caaaaagatt ttgcaagtaa
      361 cagagaagac cctaattgtt ggtgtggaca tagccagcga aactcactac gccagggctt
      421 tcgactaccg gggtatagag gttggcaagc tgcttaaatt cagcaatgat gctgagggtt
      481 ttggacaatt catagcctgg gttggctcga ttgaacaaaa acaatgcaaa aataaagtta
      541 tgataggaat ggaaccgaca ggtcattact ggtttaatat tgctcagcaa ctcaaggatc
      601 acaatctaaa gctagtactg gtgaatccgt ttcatgtaaa aaggagcaaa gaactggatg
      661 ataacaatcc aaccaaaaac gaccgtaagg atccaaaaac catagctatg ctggtcaaag
      721 atggccggta catggagccg tatattccgg aaggtattta cagcgagcta agaatagcta
      781 tggaaaccag gtggcaaata gtaaaacaac taaacagaat ccgtaaccgt atgaagcgtt
      841 ggctcagcat atactttcca gagttcaacc aggtatttgc taactgggaa ggcaaggcag
      901 ccctgatagt gttgcaggac tttcctaccc ccacgaggat actggaaaga ggcgtagaag
      961 gcattgtagg gcgttggaag caagacaagc tgagaggaat aggcagaaag cgcgctatgc
     1021 gcctgataga ggccgctaag caatctgtgg gtgtacggga aggccttatg gctgcggaaa
     1081 atgacctgct caacatgctg gagaactaca aactgcaaat gcagcattat gaaaaaacca
     1141 tgaatatggt ggagcagctt gtgaagcaaa tacccggcct ggctgagatt gtgaagatta
     1201 aaggtgtggg tttagtaact ggggcaggct tcgttgctga agtaggtgat attagccgtt
     1261 ttgaacaccc caagcaaatt cagaagctgg ccggcctgaa cataaaagaa aacagttcag
     1321 gaaagcacaa aggcaagagc agtatcagta agcgagggcg gcgccggtta agatcactgc
     1381 tgttccaagt agccatgccg ctggtagcca acaataaaga atttcaggaa ctgcacagct
     1441 actacacaac caggaaccaa aattcgttga aaaagaagca atcgttggta ctgttaagtt
     1501 gcaagctaat ccggatattt ttcgccttgt taactaaaga tatcgcctat gacccggaaa
     1561 agatgatgag tgacataaaa agaccactaa tgcaggacgc agcctaaata cgaaccgttt
     1621 aaagatagct tcggataaaa gacgcacatt gacaaaacga gccgggatta gttaggttga
     1681 ataaaatcca taagggcaaa gacccagttt agaagcatga ctaacatcca cctcatggat
     1741 aggcacaacg aaggaattta gggcacagac cctgcgagac atgggaggtt tgccaccgga
     1801 gagcaagtgg atatccaacg gccgaaataa gcttaaaaca acaggtgctt gtttaggtca
     1861 cccttgtcca tccaggttta tgcataaatg agcatgttat ccatgtggca cgatttggag
     1921 agtgcgaaat actaagaaat acaaagctat caagagaaaa ccttaggtta tagagggagg
//
LOCUS       DASRMS010000192         1097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_50969, whole genome shotgun sequence.
ACCESSION   DASRMS010000192 DASRMS010000000
VERSION     DASRMS010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50969"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(465..>1097)
                     /locus_tag="VFC74_09155"
     CDS             complement(465..>1097)
                     /locus_tag="VFC74_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25535.1"
                     /translation="KLIDGGKSDNLRGFSGRQLAWHRLEDLRTLMSLRGGVAEGDRML
                     HLHWQTNFLLLSGATNSRLMYHEAATLARAIDDGWGYNCKELMTLYSKAKQFEAGEKV
                     SFGGKEYPALYTPRNDTLINLFEITDDEQRELRTIISKGMAAERHRDRDTERRRAAGA
                     VDRETYLKAASNKQAQAQELRAQGLSVRAIAEQMDISKTAVGRYIQPGKV"
BASE COUNT          277 a          302 c          224 g          294 t
ORIGIN      
        1 catctgtgct gcaagctgga ccacgtagaa cgctgcacag cgaccgattg aacactaatc
       61 ctagacaacc ccatgcaaga ctctaaagag cagtttagag ggctaccagc ggctttctat
      121 ctgcaagcta tgtatgtgtt agtaatgaac tgctataaac ccactccctt tcattactaa
      181 caacagatat aaatatcttt ttagaagcct aagagcagaa ccgcaagagc ccgtaggctt
      241 cgccattagt aatacgcagg gacttgggac actacccttt tcgacattga aataagcaac
      301 cccagcaacc agttagcctg ctataagctt ctaaactgac ctctactatg gtgagcactc
      361 accttagcta ctaacaacag atataaatat cttttagaag cctaagagca aaaccacaag
      421 agcccgtagg cttcgccatt agtaatacgc agggacttgg gacactatac tttcccaggc
      481 tgtatataac ggcctaccgc tgttttactg atgtccattt gctctgcaat cgctcgtaca
      541 cttagacctt gcgctctaag ctcttgtgct tgcgcctgct tgttactagc agcctttagg
      601 tacgtttccc tatccactgc acctgctgca cgtctacgct ctgtatcgcg atctctgtgg
      661 cgctctgcag ccataccttt actgataatc gtccgtagtt ctctttgctc atcatctgtg
      721 atttcaaaca ggttgattag agtgtcgtta cggggcgtgt agagtgctgg gtactcttta
      781 ccgccaaagc taaccttttc accagcctca aactgcttag ctttactgta gagcgtcatt
      841 agctccttgc agttgtaacc ccagccgtca tcgatggctc gggctaacgt ggctgcttcg
      901 tgatacatca gacgactatt ggttgcacct gatagcagca gaaaatttgt ctgccagtgc
      961 aggtgcagca ttctgtcacc ctcggccaca ccaccgcgca acgacatcaa ggttcttaaa
     1021 tcctctaacc ggtgccaggc caattggcga ccgctaaaac cgcgtaagtt atccgactta
     1081 cctccgtcga ttaactt
//
LOCUS       DASRMS010000193         1577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_457635, whole genome shotgun sequence.
ACCESSION   DASRMS010000193 DASRMS010000000
VERSION     DASRMS010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457635"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(68..1522)
                     /gene="nadB"
                     /locus_tag="VFC74_09160"
     CDS             complement(68..1522)
                     /gene="nadB"
                     /locus_tag="VFC74_09160"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009985008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HZK25536.1"
                     /translation="MKGKYDVIIAGCGVAGLYAALQFDNNTNILLLSKQEKTVSGSSL
                     AQGGVAAVLDHEDDSFDLHIEDTLIAGRYKNEYSAVEVLISEGPQDIKRILDMGVDFD
                     RDKNGKLLKTLEGGHCRRRIVHHKDQTGKEMVDKLLAAVKQRPNIDLQENAMICSTAK
                     LYNGFRLIILQNNEYLNVCSSYMIIATGGIGRVYQFTTNPSIATGDGIRFAYEMGAKI
                     KNLSFVQFHPTAFNSPNSREQFLISEAVRGEGAYLLNNDLERFMHKYDERLELAPRDV
                     VSRSIILESRRTGSNNFYLDIRHRGKEFVNKRFPAISDACRQRGVNMAEDLIPVFPCQ
                     HYLMGGIDVDLNSRTTVPRLYAAGECAHTGVHGGNRLASNSLLEALVFGRRAAHDIMR
                     SLCSGYPPVEVADYGINPKGAPLPKGFRTQIREIMQRAYFVMPDEKAIHEGHRRLEEI
                     LTRLKSGRFALTTNFCEALSLATVATIILREVDE"
BASE COUNT          402 a          359 c          330 g          486 t
ORIGIN      
        1 tctatatagt ttatatcctc tttgattgcg atttttatca aatcatcaat ataaaaattg
       61 tttagcatta ttcgtcaacc tctctaagaa ttattgttgc aactgttgca aggcttaatg
      121 cttcgcaaaa gttggtagtc agtgcaaaac gaccgctttt aagccttgta agtatttctt
      181 caagcctacg gtgcccttca tgaattgcct tttcgtcggg cattacaaag taggcacgct
      241 gcataatttc tcttatctgt gtgcgaaaac cctttggcaa aggtgcgcct ttagggttaa
      301 ttccgtaatc tgcaacctcc acgggaggat aacctgagca gagagaacgc ataatatcat
      361 gtgcggcccg cctgccaaaa acaagtgctt caagcagtga attacttgca agccggttgc
      421 caccgtggac gcctgtgtga gcacattccc ctgccgcata cagtcgcggc acggtggttc
      481 tgctgtttaa atccacatct attccgccca ttaggtaatg ttggcacggg aatacaggga
      541 ttaaatcctc ggccatatta acgccccttt gacggcaggc gtcactgatt gccgggaaac
      601 gcttattaac aaactccttg cccctatggc gaatatcaag ataaaaattg ttgctgcccg
      661 ttctgcgtga ttcaagaatt atggagcgtg agacaacatc acggggggca agctcaagtc
      721 tttcgtcata tttatgcata aagcgttcaa ggtcgttatt gagaagatat gccccttcgc
      781 ctcgcaccgc ctcgctgatt aaaaactgct ctctgctgtt tggtgaatta aatgcagtcg
      841 ggtggaactg aacaaagctt aggttcttta tttttgcgcc catttcatag gcgaatctta
      901 tcccatcccc cgtagcgatt gaggggtttg tggtgaattg ataaaccctg ccgataccac
      961 ctgttgcgat tatcatgtag cttgagcaca cgtttaaata ttcattattt tgtaaaatta
     1021 tcagtctaaa cccgttatat agttttgcgg tgctgcaaat cattgcgttt tcttgcaaat
     1081 caatattagg tcgttgttta acagcggcaa ggagtttgtc aaccatttct ttgcctgttt
     1141 ggtctttgtg gtgaacaatc cttcttctac agtgaccgcc ttcaagggtt tttaaaagct
     1201 tgccattttt gtctctatca aaatcaacac ccatgtcaag aattcttttg atatcctgtg
     1261 ggccttcgct tatgagaact tcaactgcgg aatattcgtt tttatatctg cctgcaataa
     1321 gagtgtcctc aatgtgcaaa tcgaagctgt cgtcttcgtg gtcgagaaca gcggcaacac
     1381 cgccttgagc aagggagctg cctgaaacgg ttttctcctg ctttgagagc aatagaatgt
     1441 tggtattgtt atcaaactgc aatgcagcat aaaggccagc aacgccgcag ccggcaataa
     1501 ttacatcata cttgcctttc attcgtatcg tctccctatt attacagatg ggtttttacc
     1561 acaataccat tatatat
//
LOCUS       DASRMS010000194        17233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_526642, whole genome shotgun sequence.
ACCESSION   DASRMS010000194 DASRMS010000000
VERSION     DASRMS010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 17233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17233
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526642"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            95..171
                     /locus_tag="VFC74_09165"
     tRNA            95..171
                     /locus_tag="VFC74_09165"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:129..131,aa:Met,seq:cat)
     gene            188..262
                     /locus_tag="VFC74_09170"
     tRNA            188..262
                     /locus_tag="VFC74_09170"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:221..223,aa:Glu,seq:ttc)
     gene            470..1981
                     /locus_tag="VFC74_09175"
     CDS             470..1981
                     /locus_tag="VFC74_09175"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HZK25537.1"
                     /translation="MENKQTDANENKITADSEKDESSYQQGKNSWAGMRHQLKTMAAS
                     CLTRLKRTSSQVKYSALVVLVFALLVTAGSVSAYYDSFTYVVYLDDQEIGYVASETVI
                     PEFAAAWQQEQSEALGMELVTEQKLKVVHEQRKGISEDEEAVKTQLREQLDFAVYAYM
                     LMINDKPALAVATTAEYNQIIEQLKDTYVCEQKNAVVKNVVLNDKVEARLTLVEPDEI
                     YSVSKAVEILCYGTNKRQTYLVSRGDSLWSIAAHNELSVTDIRHANPQLAESDSLMPG
                     DSLNLVVSEPLVSVQVTQDVVETEAIAFETKTEKDNSMYKGTSKVLQNGKKGKKEITY
                     SVTLENGKQIAKEVIGEKVLSKPTEKIVAQGTKAVPVPSAVSGARGTGLFLWPVAGRG
                     TITSRYGYRGRGFHRGLDIGTPTGTAILAADSGTVTQSGWSGAYGILVTINHGNGYVT
                     KYAHNSATLVSVGQHVQKGQQIARSGSTGNSTGPHLHFEVIKNGSNVNPLSYL"
     gene            2260..3120
                     /locus_tag="VFC74_09180"
     CDS             2260..3120
                     /locus_tag="VFC74_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25538.1"
                     /translation="MKTQRLPRQTIPIVALCLLLLTLPTLPSAAQSTVRYFAALATRW
                     PYRAWQEQETDYFQLRFTESDQALAAWLGQQADQAVEQATQLLPYEQEHKPWLVIAPH
                     QAMLQKAFGWGENTGALGVYIAETIIILSPQAWEWEQPSLREAVFARQGPLVHEYTHF
                     LLDQSAQGNYPRWFSEGLAQYVEFQVLGYEWLEAGSSLANPLYSLQELTRQFDHLPNQ
                     ALAYRQSLSMVSFLAARGDTGLNRLITLLGQGKHFDAAVAQVYGLRPEALMTAWQHWI
                     AGDSRWLQIK"
     gene            3281..3979
                     /locus_tag="VFC74_09185"
     CDS             3281..3979
                     /locus_tag="VFC74_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK25539.1"
                     /translation="MARGKILVVDDEKPIAEILRYHLAQEGYEVSLAFDGVDALKQAR
                     NSQPDLILLDIMLPKKDGFEVCQEIRRFSNVPIIMLTAKDTELDKVLGLELGADDYVT
                     KPFSAREVLARVKANLRRTAQQQDVQAEILTCQDLQVDTGSMEVRQAGQLVEITYREY
                     LLLTYLMRHVGFVISRETLLEKVWGYDFYGDERTVDVTIRRLREKIEPKPSEPRYILT
                     KRGVGYFMGTKSNV"
     gene            3972..5786
                     /locus_tag="VFC74_09190"
     CDS             3972..5786
                     /locus_tag="VFC74_09190"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25540.1"
                     /translation="MFKSIQWKLVLIYSLLILFAMQFFAVFLTQSLEDYYVTSFAQNL
                     ESQGSLLVNFLQRYLAEDEVSTTAGDIDSLVLEYSRYAGTTDIMVLDNFGRVISATRQ
                     SNELRGQRIIQEEITQALTGTRSEATRLDPETKARMKYLALPIRVGDRNQGVVYLIGS
                     LEPIYDTLREIQLIFLTGALLVLGVTVLLGFVLAKTITGPIQEVTSKAAQIAHGDYRQ
                     RITTHADDEIGRLGEMFNYLSRQLEATLQEISSEKGKVEAILNHMTDGIIALASDGQV
                     LHVNPTAKRLLGLKIEAEPAMEALAQLIMPINLAELLATGQQTSQELSLAPEQRHIQA
                     NYVPFQTQEMEPGYYTGILIVLHDVTQERELIRRQREFVANVSHELRTPLTTIKSYTE
                     ALIDGARLQPELCLSFLTTMEKETDRMVRLVKDLLALSQIDYRQAGWHKENHALDELI
                     AEVIEELEVKYKAAPRHILVSLPVQPVTVYLDRDKMKQVLLNIIHNAYKYTAEGGQIT
                     ITLTEQPELAEIQVIDDGIGIPAADVERVFERFYRVDKNRSREYGGTGLGLPIARELV
                     AAHGGRISLTSELQRGTTVTIKLPLPEAKGDSEVCESA"
     gene            5771..7069
                     /locus_tag="VFC74_09195"
     CDS             5771..7069
                     /locus_tag="VFC74_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25541.1"
                     /translation="MRERIKSVILFVLIITSVFLTTKLLFGRPYWETASPPAYEQVTF
                     GDLRPLQEQILPVLRLGEDVDWLQLQPWDEGYQEAWEWLNWLFRPRQAPTTTSLPDFT
                     GLQVRLDFSLPVEPNWWVTNRQLNGLEIRQAVWFAEAPQAVWYQAANGKWLRGELTLP
                     EQWESQLNELFAVGLKLRAAAAEELAIQPAAGSVLLPQEVPRLAVHTITAENLETEKL
                     LGSFFVNLALVRRIEEGDGAVIYTDGQKGLRIFSHGELEFNAPENIPGANPQSLVDTL
                     QQGAQYLQLMGGWPDHLYLQKAQLTAQSIKWEQRYTQELIFQSVQKGVPLLGTNPTLR
                     LYFSDRGVIYYHRQIYLLGSPVGTPSPLVKPHQAVAAVAAALGTAVEEQRLVAVSPVL
                     YFGSKATRQSLARPVWQVQLQDEQTAVVDGFTGEFITWLE"
     gene            7083..7925
                     /gene="yycI"
                     /locus_tag="VFC74_09200"
     CDS             7083..7925
                     /gene="yycI"
                     /locus_tag="VFC74_09200"
                     /inference="COORDINATES: protein motif:HMM:NF021186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HZK25542.1"
                     /translation="MDLSKAKTLLILAFLSLNIFLGYKLWIAPQALHQGSVLTEEQLA
                     QAKALIKEHGFELKTTLPKQIPRLALLNVSRLATDDSHWLAAFWGEEKVQSRRETDKT
                     VYTAGQEALTILADGHLVYTNNLPGTSEANTRSLAERYLREWQLWQGNMKLDLAIPWG
                     EHGYRYRFVQNYQGFPLFFSAIEVYLAEGRPREVHLYQALPHGFSDKEATVISAQKAV
                     ETFMREAAEISNKSIIDISLGYYSLSYDAERWESAPVWRIATQGGSESYINAFTGEIE
                     KSTL"
     gene            8234..9490
                     /locus_tag="VFC74_09205"
     CDS             8234..9490
                     /locus_tag="VFC74_09205"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0019277 - UDP-N-acetylgalactosamine
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HZK25543.1"
                     /translation="MPRLLVTGGHPLAGAVKISGAKNAAVAIIPAALLTEAPVRIENL
                     PDIDDIHVLVEILDYLGVRVEWETPTTLFIDASTLTRKCQPPYELVKKLRASYYLLGA
                     LLGRFARAEVPVPGGCDLGPRPIDQHIKGFVSLGAKVETEHGLIKITADKLVGTDIYL
                     DVVSVGATINIMLAAVRASGKTIIENAAKEPEIVDVANFLNAMGAKIRGAGTDVIKIT
                     GVEELGSVTHCVIPDRIEAGTYMIAAAATQGNVLLQDVIPKHLDAITAKLREMNITVE
                     VRDDQLRVIGATRPQAVDLKTFPYPGFPTDMQSLVMVLLTQAEGSSIITENIFEGRFK
                     HVDELKRMGARIKVEGRTAIVDGCTTLTGAPVRATDLRAGAAFIIAGLIAQGDTIISE
                     TSHIYRGYEHVCEKLSSLGAKIREIE"
     gene            9505..10122
                     /locus_tag="VFC74_09210"
     CDS             9505..10122
                     /locus_tag="VFC74_09210"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HZK25544.1"
                     /translation="MRILLARHGETTANAEARFQGSRDYPLNERGRQQAEQLASLLAH
                     YQPGRLYTSNLTRSRETAKPAAQLLKLKAIALPIFREYSWGVLEGLTRAEIKERYPAL
                     FSRLQENWRQAVIPQQEPLPHFRQRLQAGSSILLAADNPSTVALIGHGRYLNALLVEL
                     LGLDFTGPWPFSFLSGTLTILDCQAGQCRLISFNQQSQLRGDKNA"
     gene            10115..10903
                     /locus_tag="VFC74_09215"
     CDS             10115..10903
                     /locus_tag="VFC74_09215"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK25545.1"
                     /translation="MLEICMLASGSSGNAIYVATGETKILIDAGLSGKKIAAALCAVG
                     VEASELSALLLSHDHVDHTSGAGVMARRYGLPVYATEPTWQAAGKLMGRIAAEQCCKL
                     TNCGTITLGDLTVETIPVPHDAADPVGFVFRHGPHAIALVTDLGYVTPYILQRLQAMD
                     CLVLEANHDEEMLRQGQYPWPLKKRILSDKGHLSNNLAAASLSQVISEQTQHVVLAHL
                     SEQNNCPQLAFDTVCCHLEETGQLKNLAVHIAERHLPSCHIRLA"
     gene            11026..12195
                     /locus_tag="VFC74_09220"
     CDS             11026..12195
                     /locus_tag="VFC74_09220"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HZK25546.1"
                     /translation="MDFFNGFSHQPRRAGLLGFLLTALFGAIVGGLLVALIVVQVVIP
                     GLAQAQAPNDTNVVPGSQVQQPDISFNENDLPEYQNTAIVRATQKVMPAVVGITNKAM
                     VYDMWQGRSLLQDKATGSGVIIDANGYIVTNNHVIDGAAEIFVTLGDGQEHQAELIGA
                     DPATDLAVIKIDQTGLPVADLGDSDTIVVGEQALAIGNPLGLNFSQTLTVGYISAKRR
                     TITMNEYTFTFIQTDAAINDGNSGGALVNLNGEVVGINTAKIKIAGVEGMGFAIPANT
                     VKIIAQELISNGKIVRPWLGVYAGGDIDETLAKQLQLPVDYGVLVQDVVEDSPASRAG
                     ISRGDVLISMAGKKVETFKDLRDLIYQQKVGAIVEIVLIRNGKESKTAATLTAME"
     gene            12329..13225
                     /locus_tag="VFC74_09225"
     CDS             12329..13225
                     /locus_tag="VFC74_09225"
                     /inference="COORDINATES: protein motif:HMM:NF013811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25547.1"
                     /translation="MHIILFFIDGLGLAAEGAANPLATTPLPCLQQLLEGKSLTSEAV
                     GTSTATTTLLALDATLGVPGEPQSATGQTSLFTGVNAAQAVGYHVRGFPTAPLQKILA
                     AEGILKKITALNKKATFLNTYRPQYFQQPPQDRQAAATTLLSLAAGLPLHTIDDLLSG
                     RTLSADITSELWREQGLPVPYVPPLEAGQRVTANAQQYDFVFYEYFLTDHFAHKRAQA
                     QLAQSLTNIDGFLAGMLQKLDLADTMLIVTSDHGNLEDNTSSLHTHNPVPLLLVGAGR
                     EQLPSLTDLTDLTPFILTKLQS"
     gene            13256..13735
                     /gene="rlmH"
                     /locus_tag="VFC74_09230"
     CDS             13256..13735
                     /gene="rlmH"
                     /locus_tag="VFC74_09230"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012198941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HZK25548.1"
                     /translation="MQIQILTVGKIKEKYLAAGIKEYLKRLSAYAKVEIREVKDEKTA
                     ENASAAEIKLLLEKEAARLEPLLHPQTYLIALAIEGQQFTSPDFADHLQQQTIHGKSH
                     FTFLLGGSLGLAPQLLVKADLKLSFSSLTFPHQLFRLMLLEQLYRAFKINRGEPYHK"
     gene            13807..14157
                     /locus_tag="VFC74_09235"
     CDS             13807..14157
                     /locus_tag="VFC74_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HZK25549.1"
                     /translation="MSKHQIYTMSVARVYPLYVTKAEKKGRTKTEVDEIIRWLTGYSQ
                     EELEAQLEKQVDFENFFVQAPQLNPLRSLIKGVVCGVRVEDIAEPTMREIRYLDKLID
                     ELAKGKAMTKILRK"
     gene            14280..14714
                     /locus_tag="VFC74_09240"
     CDS             14280..14714
                     /locus_tag="VFC74_09240"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HZK25550.1"
                     /translation="MMLQFFATLRGSEEVPPVATNATGNASFKVSADQRAIHYQLTVN
                     NLANLTEAHIHLGRRGENGPVVVFLFGPVDPAITVSQGVVRASFSERALVGPLAGQPL
                     AALSAEMQVGNTYVNVHTKRHPAGEIRGQISHSKQAGSARDE"
     gene            14929..15561
                     /locus_tag="VFC74_09245"
     CDS             14929..15561
                     /locus_tag="VFC74_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013240368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HZK25551.1"
                     /translation="MAMSAKSSDYFLDELASKAPTPGGGGAAAMGGAIGMALSNMVGN
                     LTIGKKKYAAVEEEVKALVEQGYKIITELKALVDKDAEVFAPLSKAYGLPKDTPEQAA
                     HKAKVLEDCSKLACSVPMEIMRKSYEGIKIHERMGEIGTKLALSDVGCGVTFLKSALI
                     SGYLNVLINVNTIKDEQFVSETFAETSHLIEDGSKVADATLQKVIDKLKK"
     gene            15594..16451
                     /locus_tag="VFC74_09250"
     CDS             15594..16451
                     /locus_tag="VFC74_09250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HZK25552.1"
                     /translation="MAEQLRGKAVANAMKEDLAARVEALKAKGVTPKLGFIRVGARPD
                     DLFYEGGAKKTADGIGLTYEVFEYAEDVAQADFEQAVTEIGGRKDINGILMFSPLPKH
                     LDEKKIRSLIPVEKDVDCLTVHGAGKVFADDQTGFPPCTPTACMDILKFYDVPLHGKK
                     VVVLGRSMVVGKPVAMLLLRQNATVTICHSRTENLPAVCAEADVLVAAVGRPKMVNAE
                     YVRAGQTVIDVGINPDPDRPGKFCGDVDFAAVEPIVEKITPSPGGVGSVTTMVLSKHT
                     IMACEMQNA"
     gene            complement(16543..>17233)
                     /locus_tag="VFC74_09255"
     CDS             complement(16543..>17233)
                     /locus_tag="VFC74_09255"
                     /inference="COORDINATES: protein motif:HMM:NF019350.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HZK25553.1"
                     /translation="TEPLLRIALRSKEVGAKEVFIPLHLNEYLSPKQYNEFYWPTLKE
                     VIVKLFELHDSISQGVQGLIFALHSLRHELDGPPQRVADILQHLEATARSTLDELRAL
                     IEELKPSLLVEQGLAASIQAASELFSQRQTIPVELAYELTALLSPEVEMTIYRIIQEA
                     LANIEKHAQAQQVTIRISDLEGQVLLSVKDDGQGFSLPRALRWADSTLRAAFMDGWLG
                     NAALSGRHTMH"
BASE COUNT         4744 a         3739 c         4224 g         4526 t
ORIGIN      
        1 ggatggagca gtctggtggc tcgtcgggct cataatccgg aggtcgttgg ttcaaatcca
       61 actcccgcaa ccatattttc aacaataaag tcctggcggc gtagctcagt tggctagagc
      121 atacggttca tacccgtagt gtcagcggtt cgactccgtt cgccgctacc atctaacagc
      181 tttcattggc cccttggtca agcggttaag acaccgccct ttcacggcgg taacgcgggt
      241 tcgagtcccg caggggtcac catgaaaaag ttaagagacc ttaacaggtc tccttttttt
      301 ttaacttttt taagaaaagt taatatttcc ttaactttta gcaggaatat taaaataacc
      361 tgccgaatat agctaaaggt taatttttaa aggcgtcact ttatgcgtaa caaaatagac
      421 aacataagac ggacagcgag gcgacaaaga taaaatatga ggtgatttta tggaaaacaa
      481 acaaacggat gctaacgaaa ataaaataac ggcggattca gaaaaagatg aaagctctta
      541 ccagcagggt aaaaactcct gggcagggat gaggcatcaa ctaaaaacta tggcagctag
      601 ttgcctgaca cgcttaaaaa ggactagcag ccaagtaaaa tatagtgctt tggtagtttt
      661 agtttttgct ttactggtaa cagctggcag cgtttctgcc tattacgatt cctttaccta
      721 tgtagtatat ttggacgatc aagaaattgg ttatgtggct tcagaaactg ttattccgga
      781 gtttgcagca gcctggcagc aagagcaaag tgaagcgctg gggatggaat tggttactga
      841 acaaaaactt aaagttgtac atgaacaacg aaaaggtatc tctgaagatg aggaagcagt
      901 taaaacacaa ttacgtgagc agctggattt tgctgtttac gcttatatgc tgatgattaa
      961 cgataagcct gcgctggcag tggccactac tgcggaatat aatcagatca tcgagcaatt
     1021 aaaagataca tatgtttgtg agcaaaaaaa cgcagtcgtc aagaacgtag ttttgaacga
     1081 taaagtggaa gcaagattaa cactagtaga gcccgatgaa atctatagtg tcagtaaagc
     1141 tgtggaaatt ctctgctatg gcacaaataa acgtcaaaca tatctagttt cccgtggtga
     1201 ttccctttgg tctattgccg ctcataatga actgtctgtc acagatattc ggcatgctaa
     1261 tccccagcta gctgaatcag acagcctaat gcctggtgat agcttaaatt tagttgtttc
     1321 tgagccccta gttagtgtac aggttactca ggatgtcgta gaaaccgaag caattgcctt
     1381 tgagaccaag acggaaaaag acaactccat gtacaaaggt accagtaaag ttctccaaaa
     1441 tggtaaaaag ggtaaaaagg aaataaccta cagcgtaacc ttggaaaatg gcaagcaaat
     1501 tgcaaaagaa gtaattggtg aaaaagtatt gtcaaagccc acagaaaaaa ttgtggccca
     1561 aggtacgaaa gctgttcccg tgccgtctgc agttagcggc gcccgtggga caggcctttt
     1621 tctctggcca gtggccggac gcggcacgat tacttctcgc tacggttatc gcgggagagg
     1681 tttccaccgc ggtttggata ttggtacacc taccggtaca gcaattttag ccgctgacag
     1741 cggcacagtt acacagtcag gctggtcagg tgcgtatggt attttagtga cgatcaatca
     1801 tggcaacggt tatgttacaa agtacgcaca taattcagcg acattagtca gtgtggggca
     1861 gcatgtacaa aaaggccagc aaattgcccg ttccggcagc accggtaatt ccaccggtcc
     1921 ccatttacac ttcgaagtaa tcaaaaacgg ttctaatgtc aaccccttgt cgtatctcta
     1981 aattatcacg agcacttcat tgagaagtgc tcgttttatg tttgataata attacctggt
     2041 gtcacgaaat cataaaatca ctgtaatatg cttgtaatat tcgttcgcta taataaagac
     2101 aaggtttgac cccctcttta aatatatttt tcttctaacg tggtgcgcca cgttattttt
     2161 ttgcccacct ttttgcagaa actggcttgc tttaccaatg aaaatcgctg ctagacctgt
     2221 tacaatattt aagcaggcat caagggggga ggcagaccag tgaagacaca aagattacca
     2281 cgacaaacca taccaatagt cgccctttgt cttttgctgc tgaccttacc tactttgccg
     2341 tcggcagccc aaagcacagt gcgatatttt gctgctctgg ctacccgctg gccttatcgt
     2401 gcctggcagg agcaagaaac agattatttt caactacgtt ttacagaaag cgaccaagct
     2461 ttggcagctt ggttgggtca gcaggctgat caggcggtag agcaggccac tcagctccta
     2521 ccgtacgagc aagagcataa accttggcta gtcatcgctc cgcatcaggc tatgctgcaa
     2581 aaggcttttg gctggggtga aaacacaggc gccttaggcg tttatatagc cgagacgatt
     2641 attattcttt ctcctcaggc ttgggagtgg gagcagccca gcttgcgaga ggcagttttt
     2701 gcgcggcagg ggccgcttgt gcacgagtat acccattttc ttttagatca aagtgcgcag
     2761 ggtaactacc cacgttggtt cagtgaaggt ctggctcagt atgtggagtt tcaagtgctg
     2821 ggctatgaat ggttagaagc aggcagttcc ctggctaacc ctctgtattc gctgcaagaa
     2881 ttaacacgcc aatttgatca cttgcctaac caagctctcg cctatcgtca gtccttaagt
     2941 atggttagct ttttggctgc acggggcgac actggtctta atcgcttgat tactctgctt
     3001 ggtcagggca aacactttga tgctgcggta gcacaggttt atggactaag gccagaggca
     3061 ttgatgacgg cctggcagca ctggatagcc ggtgattcgc gctggctgca aataaaatag
     3121 taaccaaagc gtgttctttt cagttttctg aaagcacgct tttactttag agcactatct
     3181 gcatggcgca cgcagcgcac caaccaacta ctagattatg taaagcagga aattaagttc
     3241 tgaaacagaa gtatttagta atttgggaag aggtgatcat gtggcgcgtg gcaaaatcct
     3301 agttgtggat gatgaaaaac caattgcaga aattttgcgt taccatttgg cacaagaagg
     3361 ctatgaagta agcttggcct tcgatggtgt ggacgcgcta aaacaagcac gaaactcgca
     3421 gccagatctc attctgctcg atatcatgtt acctaaaaaa gatggctttg aggtctgcca
     3481 ggaaattcgt cgtttcagta atgtgccaat tattatgtta acagctaaag atacagaatt
     3541 ggataaggtc ttgggtttgg aactaggtgc tgatgattat gtcacaaaac ctttctccgc
     3601 acgtgaggtt ttagcccggg tcaaggctaa tctgcggcgt acggcacagc agcaggacgt
     3661 acaggcagaa attctcacct gtcaggatct acaagttgat acgggcagca tggaggtacg
     3721 gcaggccggg cagctggtag aaattaccta tcgggaatat ttacttttaa cctatctgat
     3781 gcgccatgta gggtttgtga tcagtagaga aaccttactg gagaaagtct ggggttatga
     3841 cttttatggt gatgaaagaa cagttgatgt taccattcgc cgtttgcggg aaaaaatcga
     3901 acctaaacca agtgaaccac gctatatttt gactaaacgc ggggttggtt actttatggg
     3961 aactaaaagt aatgtttaaa agtatccaat ggaagcttgt gctgatctat tctttactca
     4021 ttttatttgc catgcaattt tttgctgttt ttctcactca gtccctggag gattattatg
     4081 ttaccagctt cgcccagaat ttagaatcac agggctcgtt gttagtcaat tttttgcagc
     4141 gttatctagc tgaagatgaa gttagtacta ccgctggaga cattgatagt ttagtgctag
     4201 aatactcgcg ctacgccggc acgacagata tcatggtgct ggataatttc gggcgcgtta
     4261 tttccgctac acgccagagt aatgagctgc gggggcagcg tattattcaa gaagagatca
     4321 cacaggcgct cacgggtaca cgtagtgaag ccacacgttt agatccagaa acaaaggcac
     4381 ggatgaagta tttagctctg cccatacgcg tgggtgatag aaaccagggc gttgtctatt
     4441 taattggctc acttgaacca atttatgata ctttacgtga aattcagctg atttttttaa
     4501 ctggtgcctt gctggtgctg ggcgtgactg tacttttagg ttttgtctta gctaaaacga
     4561 ttactggacc tattcaggaa gtgacctcta aagcggcgca aattgcgcat ggcgattatc
     4621 gccaacgcat tactacgcat gctgatgatg aaatcggccg gttgggggaa atgtttaatt
     4681 acttatcccg gcagctggaa gcaaccttac aggagatatc atcagaaaag ggtaaagtgg
     4741 aagcgattct caaccatatg acagatggga ttattgcttt ggccagtgat ggtcaagttt
     4801 tacacgttaa cccaaccgcg aaacgactac tcggtttaaa aatagaagca gaaccagcga
     4861 tggaagctct agcccaactt attatgccga ttaatttagc tgaattatta gctacaggtc
     4921 aacaaacaag ccaagaatta agcctggcgc ccgaacagcg tcatattcag gcaaattatg
     4981 tgccttttca aactcaggag atggagcctg gctattatac agggatcctg attgttttgc
     5041 atgatgtcac acaagaacga gaactaattc gtaggcagcg ggagtttgta gccaatgtct
     5101 cccacgaact gcgtacccca ttaactacca tcaaaagcta cacagaggcc ctaatagacg
     5161 gggcaaggtt acagccagaa ctgtgtcttt ccttcttgac cacgatggaa aaggaaacag
     5221 accggatggt gcgcttagta aaagatttat tagctttatc acaaatagat tatcgccaag
     5281 caggctggca taaagaaaac catgctttag acgagctgat tgctgaagtg atagaggaat
     5341 tagaagttaa atataaagcg gccccgcgtc acatcctcgt ctcactacct gtacagccag
     5401 tgacggtgta tctcgaccgg gataaaatga agcaggtcct gctgaatatc attcataatg
     5461 cttataaata tacggcagag ggtgggcaga ttaccattac actcaccgaa cagccagaac
     5521 tagcagaaat tcaagtgata gatgatggta ttggtattcc cgctgcagat gtagagcgcg
     5581 tgttcgagcg tttctaccgg gtagacaaga atcgctcccg tgagtatgga gggactggtc
     5641 taggcttacc cattgcccgt gagctagtag ccgcacacgg tggcaggatc agcctgacaa
     5701 gcgagctgca gcgcggcacg acagtgacca ttaagctgcc cttgccggaa gcaaaggggg
     5761 atagcgaagt atgcgagagc gcataaaatc agttatccta tttgtcttaa ttatcaccag
     5821 tgttttttta acaacaaaat tgctttttgg gcggccctac tgggagactg cttcaccgcc
     5881 agcttatgaa caagtaacat ttggtgattt gcgtcccctc caggaacaaa tcctgccggt
     5941 gttacgtcta ggagaagatg tggactggct gcagctgcag ccctgggatg agggttatca
     6001 ggaagcctgg gagtggttaa actggctgtt taggccgcga caagcgccca ccacaacttc
     6061 cttgcctgac tttaccggct tacaagtacg actagacttt tctttgcctg tggagcctaa
     6121 ctggtgggtt actaaccgtc agcttaacgg tcttgaaatt cgacaagcag tctggtttgc
     6181 tgaagcacca caagccgtct ggtatcaggc agccaacggc aaatggctgc gtggtgagtt
     6241 gacactgcct gagcagtggg agtctcagct gaatgaacta tttgctgtcg gccttaaact
     6301 ccgtgccgct gctgcagaag agctggctat acaaccagcc gctggcagtg tgcttttacc
     6361 gcaagaggtg ccacggcttg ctgtgcacac cattactgct gaaaacttgg aaactgaaaa
     6421 actgcttggt agtttctttg tcaatctggc tttagttaga cgtattgagg aaggtgacgg
     6481 agcagtcatc tatactgacg ggcaaaaagg tttacgcatc tttagccatg gcgagctgga
     6541 gttcaatgcg ccagaaaata tacctggtgc taatccccag tcgctggtcg ataccttgca
     6601 gcagggtgct caatatttgc aattgatggg agggtggcca gatcacctct atttgcagaa
     6661 agctcagctg actgcacaat caatcaagtg ggagcaaaga tatacccagg agttaatttt
     6721 tcaatctgta caaaaagggg tgcccttgct ggggactaac ccgactttgc gtttatattt
     6781 ttcagatcgt ggtgttatct attatcaccg tcagatttat ttactgggta gtcccgttgg
     6841 aaccccatcc ccgctagtta agccgcatca agccgtagcg gcagtagctg ctgctttggg
     6901 gacggcggtg gaagaacaga gactggtggc ggtatcccca gtattatatt ttggcagtaa
     6961 agccacacga caatcattag cccgacccgt ctggcaggta caactgcagg acgagcagac
     7021 agctgtagtt gatggtttca caggtgagtt tatcacctgg ttggaatagg aggtcaggtt
     7081 acgtggattt atcgaaagcc aaaacacttt taattctggc atttttaagc ttaaacattt
     7141 ttttaggcta taaattatgg atagcgccgc aagcactgca tcagggcagt gttttaacag
     7201 aagagcagct ggcgcaagca aaggccctga tcaaggagca tggcttcgaa ctgaagacta
     7261 cactgcccaa acaaatacct cgtttagctt tgcttaatgt ttctcgttta gcaacagacg
     7321 attcccactg gctagccgct ttttggggtg aggaaaaggt gcagagccgg cgtgaaacgg
     7381 acaagacagt ctatacggcc ggccaggaag cactgacaat tttagctgac ggacatttgg
     7441 tttacactaa taatttacca ggcacaagcg aagctaatac gcggtcgctc gccgaacgtt
     7501 atctgcgcga atggcagctc tggcagggaa atatgaagct agatttagcc attccttggg
     7561 gagagcatgg ttatcgctat cgtttcgtgc agaattatca aggctttcct ctcttcttca
     7621 gtgcgattga ggtttatttg gcagaaggaa ggccgcgtga agtacatttg taccaagcat
     7681 taccacatgg ttttagtgac aaagaagcta ctgtcatttc tgctcaaaaa gcagtggaaa
     7741 cgttcatgcg ggaagcggcc gagatctcca ataaaagtat cattgatatc tcgttaggct
     7801 actatagttt aagctatgat gctgaacggt gggaaagtgc accggtgtgg cgaattgcta
     7861 ctcaaggcgg cagtgagtcc tatattaatg cctttaccgg tgaaattgaa aaatccacct
     7921 tataaaaaat aattaccaaa caggaattca ttcttttagc aagaatctat atacaagaaa
     7981 agtacacttc ttcagtttcc ttaacgtgta cttttcagta ctggcaactg catgagcttc
     8041 gctagctgcg aagtgataca atgtacaagt cagtgcaaga aaagtacact tcttcagttt
     8101 ccttaacgtg tacttttcag tactgacagc cgcatgagct tcgctaactg cgaagtgata
     8161 cagcgtgctg tcaatgccaa aaataaatct atgacgccag ctaacaggtg acgatttcag
     8221 gaggaaaatt tcgatgccta gacttcttgt aaccgggggc cacccgcttg ccggtgccgt
     8281 taaaataagc ggtgctaaaa atgctgcggt agcaattatt cccgcggcgc ttctcaccga
     8341 agcgccggta cggattgaaa atttaccgga tattgacgat attcatgtgt tggtagaaat
     8401 attagattac ctaggtgtac gagtagagtg ggaaacaccg accacgctgt ttattgatgc
     8461 ctctaccttg acaagaaaat gtcagccacc atacgagctt gttaaaaaac tgcgtgcttc
     8521 ctattatcta ctaggggctt tactggggcg ctttgctcgc gccgaagttc ctgtacctgg
     8581 aggctgtgat ttaggtccac gtcctattga tcaacatatc aaaggctttg tcagtttagg
     8641 agctaaagta gaaacggaac atgggttaat taaaataacc gctgacaagc ttgtcggtac
     8701 ggatatttac ctggatgtcg ttagtgtcgg tgccacaatt aatatcatgt tggcagcggt
     8761 acgggcaagc ggcaagacca tcattgaaaa tgcggctaaa gagccggaaa tagtagacgt
     8821 tgctaatttt ctcaatgcca tgggcgccaa aattcgcggc gctggtaccg atgtaattaa
     8881 aatcacaggc gtggaggaat taggttcagt tactcattgt gtgatccctg atcggattga
     8941 agccggtacc tatatgatag cggcggcggc aacacaagga aatgttttac tgcaagacgt
     9001 tattcctaaa catttagacg ccattactgc taaattacgg gaaatgaata tcactgtaga
     9061 ggtgcgcgat gatcagctgc gtgtgattgg ggcaactcgt ccgcaggctg tagatctgaa
     9121 aacttttcct taccctggct tcccgactga catgcagtct ttagtcatgg tgcttttaac
     9181 ccaggctgag ggcagcagta ttattacgga aaatatcttt gaaggacgct tcaagcatgt
     9241 ggatgaactt aaacgcatgg gggcacgcat taaagtggag ggacgtacag ccattgtcga
     9301 tggatgtaca acgctaacgg gagcaccagt tagagctaca gatttacggg ccggtgctgc
     9361 ctttatcatt gcggggttaa tcgcccaagg cgataccatt atcagcgaga ccagccatat
     9421 ctaccgtggt tatgaacatg tttgtgaaaa attatcttca ctaggtgcca agattagaga
     9481 aattgaataa taaaggtgaa gcatatgcgt attctgcttg ccagacatgg cgaaacaact
     9541 gctaatgccg aagcgcgctt tcagggttca cgggattacc cgctaaatga gcgcggcagg
     9601 cagcaggctg aacagttggc atcattatta gctcactatc aaccaggcag attatacacc
     9661 agcaatctga cgcgcagcag agaaacagct aaaccggcag cccagctgtt aaagctgaaa
     9721 gctatcgccc tgcccatctt tcgtgaatat tcctggggag tgctggaggg gcttacacga
     9781 gcagaaatta aggagcggta ccccgctctt ttttcccgtc tccaggagaa ctggcgccag
     9841 gctgtgattc ctcaacagga accgctcccg cactttcgcc agcgcctgca agcaggttca
     9901 tcaattctgc tggctgcaga taacccctct accgttgccc tgattgggca cggtcgttat
     9961 ttaaatgcgc tgctggtgga attactaggt ctggatttta ctggtccttg gcccttttcc
    10021 tttctgtctg ggaccttgac tattttggat tgtcaggcgg gtcagtgccg tttaatatct
    10081 tttaaccaac agagtcagct gagaggagac aaaaatgctt gaaatctgca tgctagcaag
    10141 tggatcttcc ggtaatgcta tctatgtggc gacgggagaa acaaaaattt taattgatgc
    10201 cggattaagc ggcaaaaaaa tcgccgctgc tttatgcgcc gtgggtgtag aggccagcga
    10261 attgtcagct ttactgcttt ctcatgatca tgttgaccat actagtggtg ccggcgtgat
    10321 ggcacgccgt tatggtctgc ctgtctatgc cacagaaccg acttggcagg cggcgggtaa
    10381 actaatgggg cggatcgctg cagagcagtg ctgtaaatta acaaactgcg gtacgattac
    10441 gctaggtgat ttaaccgtag aaaccatccc tgtgcctcac gatgctgccg atccggttgg
    10501 ttttgtgttt cgtcatggcc cccatgccat cgctctggtc actgatttag gttatgttac
    10561 gccgtacata ttgcagcgac tgcaggcgat ggactgtctg gtgctggaag cgaatcatga
    10621 tgaagaaatg ctgcggcagg gacaatatcc ctggccgctg aaaaaaagga ttttaagtga
    10681 caaaggtcac ctatccaaca atcttgccgc cgccagtctg tcccaagtga ttagcgagca
    10741 aactcagcat gtggtcttgg ctcaccttag cgaacagaac aattgtccac aactggcttt
    10801 tgatactgtt tgctgccatt tagaagagac aggacagtta aaaaacctag cagtacatat
    10861 agcagaacgc catctcccta gctgtcatat tcgtctggcc tagtatgtag actaaaatat
    10921 agggaaataa ttccttggta taaatatttt cgctgcttct tcatattatt gtcacaactt
    10981 actgttatat ttaaaatata gtttagttaa ggggggtcta aagcaatgga tttctttaat
    11041 ggtttttctc atcagccgag acgagcaggg ctgctgggtt ttctcttgac tgctctgttt
    11101 ggggccattg tcggtggctt gctggtggca ctcattgtcg tacaggttgt cattcctgga
    11161 ctagcccaag cccaagcccc taatgacaca aacgtggtgc ctggcagtca agtccaacaa
    11221 cccgacattt cgtttaatga aaatgatttg cccgaatatc aaaataccgc tattgtgcgt
    11281 gccacccaaa aggtgatgcc ggcggtggta ggtatcacga ataaagccat ggtttacgat
    11341 atgtggcagg ggcgttccct gctgcaagac aaggctactg gctctggtgt gattattgat
    11401 gctaatggct acattgtgac caataaccat gtgattgatg gcgccgcaga aatttttgtc
    11461 accctaggtg acggtcaaga acaccaggct gaattaattg gtgctgaccc agccacagat
    11521 ttggccgtaa ttaaaataga ccagacaggt ttacctgtag ctgatttggg tgactctgat
    11581 actattgttg ttggtgaaca agccttggcc attggtaatc ctttgggtct caatttttca
    11641 caaaccctga ccgtgggcta tattagtgcg aaacgccgca cgattaccat gaatgaatat
    11701 actttcacct ttattcaaac agatgcggcg attaatgatg gcaatagtgg tggcgcttta
    11761 gttaatttaa atggcgaagt agttggcatc aatacggcta aaattaaaat tgccggtgtc
    11821 gaaggtatgg ggtttgcaat tcccgccaac acagtaaaaa taattgctca ggaattgatc
    11881 agcaatggta aaattgttcg tccctggtta ggtgtttatg ctggcggcga tatcgacgaa
    11941 acactggcaa aacagctgca attaccagtc gattatggtg ttttagtgca ggatgtggtt
    12001 gaagacagcc ccgccagccg tgccggcatc agccgtggtg acgtgcttat tagcatggca
    12061 gggaaaaaag ttgaaacctt taaagactta cgtgatctca tttatcaaca aaaggttggc
    12121 gcgatagttg aaattgtctt gattcgcaat ggtaaagaaa gcaaaactgc tgccaccctc
    12181 accgcaatgg agtaaggtgg cggcagcttc aaccgaatta acctaattaa gcaggccgtt
    12241 ctttgtgcct gcttttttat ctggtataca gcaaccttga atttcctttc agcttaaaat
    12301 atgatgatca gaaaaagagg tagagcagat gcacatcatt cttttcttta ttgatggttt
    12361 aggtctggct gctgaaggag ctgctaatcc cctggccaca acgcccctgc cgtgtttgca
    12421 gcagctgctt gagggtaaga gcctaacgtc ggaagctgtg gggactagta cggcgacaac
    12481 caccctttta gcccttgacg cgacgctagg tgtgcccgga gagccacaaa gtgctacggg
    12541 ccagacatcc ctctttactg gtgttaatgc ggcacaagct gtagggtacc acgtccgtgg
    12601 tttccccaca gcgcctttac aaaagatttt agccgcggaa ggaattttaa aaaagattac
    12661 ggcactaaat aaaaaagcaa ctttcctaaa tacctatcga cctcaatatt tccagcagcc
    12721 accgcaggac cgccaggctg cggctaccac tttattaagt ttagctgctg gcttgccgct
    12781 gcacaccatt gatgatttac tgtccggtcg taccttaagt gcggatatta ccagtgagct
    12841 atggcgggag cagggtttgc ccgtccctta tgtaccgccg ctagaagcgg ggcagcgggt
    12901 tactgccaac gcccagcaat atgattttgt tttttatgaa tactttctga ccgatcactt
    12961 tgctcataag cgagcacaag ctcagctggc ccagagtttg acgaacatcg atgggttttt
    13021 ggcaggcatg ctgcaaaagc ttgatcttgc agacacgatg ctgatagtca ccagcgatca
    13081 cggtaatcta gaggataaca ctagctcgct gcatactcat aaccctgtac cgttgttgtt
    13141 agtgggcgcc gggcgtgagc agctgccatc cctaactgat ttgacagatc ttacgccttt
    13201 tatcctcaca aaactgcaaa gctaacaacc ttattagaaa gtatgggagg caacaatgca
    13261 gattcagatt ctcacagttg gtaaaattaa agaaaaatat cttgccgcag gcatcaagga
    13321 gtacctgaaa agactatctg cttatgccaa agtagaaatt cgtgaagtta aagatgaaaa
    13381 gacagcagaa aacgcctctg ccgctgaaat taaactgctg ctggaaaaag aagccgctcg
    13441 gctagagccg ctgcttcatc ctcagaccta tctgattgct ttggcaattg aaggtcagca
    13501 gtttacatca cctgattttg ctgaccacct ccaacagcag actattcatg gcaaaagcca
    13561 ttttaccttt ctccttggcg gcagtttagg cttagccccg cagttattag tgaaagcaga
    13621 cctgaagctg tctttctcct ccctgacctt tccccatcag ctttttcgct taatgctgct
    13681 cgaacaactc taccgcgcct ttaaaattaa ccgtggggag ccctatcata aatgagcacg
    13741 gcgagcgttt aaacgcagct ataccagact gtgaaataaa cattaagcag ctaaaggagg
    13801 ctgtcgatga gcaaacatca gatttatacc atgagtgtcg ccagggtata tccgctttat
    13861 gtgacgaagg cggagaaaaa aggacgtaca aaaacagaag tcgatgaaat tatccgttgg
    13921 ctgacaggct atagtcagga agagttagag gcacaactag aaaagcaggt agactttgag
    13981 aacttttttg tgcaagcccc ccaacttaat cctttgcgtt ctttgatcaa aggggtggtc
    14041 tgcggtgtcc gcgtggaaga tatcgccgaa ccaacgatgc gggaaattcg ttatctggat
    14101 aagctgatcg atgagctggc aaaaggtaaa gcaatgacca agattttacg taaataagaa
    14161 aaaagcaagc aacaaggctt ttgagctgaa caggccccta cggtggagaa tgctgtaaag
    14221 cttaactaaa tataatcctc ctgaatacta tgaacaaatt cgagtgtggg aggagggtta
    14281 tgatgttaca attttttgcc acactgcgag gttcagagga agttccccct gtcgccacta
    14341 atgctacggg aaatgcttcc tttaaagtca gcgcagatca aagagcaatt cattaccaac
    14401 tgacagtcaa taatcttgcc aatttaactg aagctcatat tcacctggga cgccgcgggg
    14461 aaaatggacc ggtagtggtc ttcctcttcg ggcctgtaga tccggcaatt actgtcagcc
    14521 agggtgtggt acgcgccagt tttagtgaac gtgctttagt gggaccatta gcagggcagc
    14581 cgctcgctgc tctcagcgcg gaaatgcagg tgggtaacac ttatgttaat gttcacacca
    14641 aacggcatcc tgcaggagaa attcgtggcc aaattagtca tagcaaacaa gcagggtctg
    14701 ctcgggatga atgatgaaga tgctgcatcc tagggggacg cccaagctcc ggccctagga
    14761 tgctaaaaac ggagttcgta actgggctgt gctgcgaggt ttgcaaagca gcccagtttt
    14821 tcattatcgt agtcatttct tttgattatt agtggcaatc tatagaggaa aatcgcttgt
    14881 gatgaagaat tacaagtagt atgaatcttg ttaagggagg gtttatttat ggcaatgtcc
    14941 gcaaaaagta gtgattactt cttagatgaa cttgcttcca aggcgccgac accaggtggt
    15001 ggtggtgctg ccgccatggg tggtgctatt ggtatggctc tatctaatat ggtaggcaac
    15061 ttgacaatag gtaaaaagaa gtacgccgct gtagaagaag aagtaaaagc tctggtagaa
    15121 caaggctaca agattattac ggaactaaaa gctctagtag ataaagatgc cgaagttttt
    15181 gcgcccttat ctaaagcata tggtctgccc aaggataccc cggaacaagc tgctcataag
    15241 gcaaaagttt tagaggattg ttcaaaattg gcttgctcag tgccgatgga gattatgcgc
    15301 aagtcatatg aaggtattaa aatccatgaa cgcatgggtg aaattggtac aaagcttgct
    15361 ctttctgatg taggttgtgg tgtaacattc cttaaatccg cactgattag cggctactta
    15421 aatgtactga ttaatgttaa cacgattaaa gatgaacagt ttgtcagcga aacctttgca
    15481 gaaacaagcc atttgataga agatggcagc aaggttgcag atgccacatt acaaaaagta
    15541 atcgacaagc ttaaaaagta acaaaagtaa atacataagg tgggaggatt acaatggctg
    15601 aacaattaag aggtaaagca gttgccaatg ccatgaaaga agatttggca gcaagagtgg
    15661 aagcattaaa agcaaaaggt gttactccga aactaggttt tattcgtgta ggtgcccgtc
    15721 cagatgacct cttttacgaa ggtggagcga aaaaaactgc cgacggtatt ggtttaacgt
    15781 atgaagtttt cgagtatgcc gaagatgtcg cccaagctga ttttgaacaa gctgttaccg
    15841 aaataggcgg cagaaaagat attaatggaa ttttaatgtt ctcgcctctg cccaagcatt
    15901 tggatgagaa aaaaattcgt tctttaatcc ccgtggaaaa agatgttgac tgcctgactg
    15961 tgcatggtgc aggtaaagtt tttgctgatg atcaaaccgg tttccctcct tgcacaccaa
    16021 cagcgtgcat ggatatccta aagttttatg acgtgccttt gcacggcaaa aaagtggtcg
    16081 ttcttggtcg ttccatggtt gttggtaagc ccgtagcgat gcttttactc cggcaaaatg
    16141 ccactgtaac catttgccat tcccgtacag aaaatctgcc cgcagtctgt gctgaagctg
    16201 atgtgctggt agctgctgtc ggtcggccca aaatggttaa cgcagagtat gtccgcgctg
    16261 gtcaaactgt gattgatgtg ggtattaacc cggatcccga cagacccggt aaattctgtg
    16321 gagatgttga ctttgctgca gttgaaccca tcgtagaaaa aattactccc tctcccggcg
    16381 gtgtaggttc agttaccacc atggtcctca gcaaacacac cattatggct tgtgaaatgc
    16441 aaaacgctta aataaaaata ttaactgctt gaaaaaccgc tctagcaaat gctagagcgg
    16501 ttttgtttgt cgcggcgaaa ggacctgctt taaatttcag ctttaatgca tagtgtgccg
    16561 gccactaaga gcagcgttcc caagccaccc atccataaaa gcggctctaa gagtgctgtc
    16621 agcccagcgc agtgcccgtg gtagagagaa accttggcca tcatctttta ctgacagcag
    16681 cacttgccct tcaaggtcgc tgatgcgaat cgtcacctgc tgggcttggg catgcttttc
    16741 aatatttgcc aatgcctcct ggatgatacg atagatggtc atttctactt ctggtgagag
    16801 taaggcagtg agttcgtagg ccagctctac agggatggtt tgtcgttgtg agaataattc
    16861 actggccgct tgaattgagg ctgccaatcc ttgttctacc agcagtgaag gttttaattc
    16921 ttcaattaag gcacgcagct catctagcgt ggagcgggcg gtggcctcga gatgttgtaa
    16981 aatatcagcc acccgctgcg gggggccatc taattcatgg cgtaaggagt ggagcgcaaa
    17041 gattagaccc tgtacaccct gtgaaatgga atcgtgcagc tcaaacagct tgacaatgac
    17101 ctcttttaag gtcggccaat aaaactcgtt atattgttta ggcgaaagat attcattcaa
    17161 gtgcagcgga ataaagactt cctttgctcc cacctcttta gacctcaagg caattctcaa
    17221 cagcggctct gtt
//
LOCUS       DASRMS010000195         1194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_458462, whole genome shotgun sequence.
ACCESSION   DASRMS010000195 DASRMS010000000
VERSION     DASRMS010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1194
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_458462"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /gene="hisG"
                     /locus_tag="VFC74_09260"
     CDS             complement(<1..316)
                     /gene="hisG"
                     /locus_tag="VFC74_09260"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HZK25554.1"
                     /translation="MLMDKEWLTIALAKGRILAPTLSLLTAAGINCEELSTDTRKLIF
                     TVAEQKLCFILGKPTDIPTYVEYGAADLGVVGKDVLLEQEKELYELLDLKLGVCRLAV
                     ARR"
     gene            complement(310..>1194)
                     /locus_tag="VFC74_09265"
     CDS             complement(310..>1194)
                     /locus_tag="VFC74_09265"
                     /inference="COORDINATES: protein motif:HMM:NF024785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HZK25555.1"
                     /translation="QQGKQRELTQAGVELLGSPSAASDAEVIALAAAVLQAAGLTEFT
                     LCLGHSGFLENLLSAYQVDTTERALIKHLFNKKDFVALKNQVSQLPLAAWQKENILRV
                     PSLRGGREVLTAARELLPEPQAALPLTTLAEVWSVLEEYGVTKYLQLDLGLVRLLDYY
                     TGMVFEGYTGGLGYALCGGGRYDQLLDNFGPSQPAVGFAVSIDHLITVLTKQKKLPAL
                     SLPVDVAYGAGGRAAAVAVVQALRAAHTTAALAVAEVSAAEARRQSTARGAQRLLYYS
                     DAGVLDLDLQKEAGESRC"
BASE COUNT          324 a          331 c          240 g          299 t
ORIGIN      
        1 cccggcgggc aactgccaac cggcaaacgc ccaactttaa atccaacaat tcatataatt
       61 ctttttcttg ttctaacaag acatctttac caactacccc taaatctgca gccccatatt
      121 cgacataggt agggatatct gtgggtttac caagaataaa gcagagcttt tgttcagcaa
      181 ctgtaaagat tagttttctg gtatccgtag acagttcttc acagttgatg ccggccgccg
      241 ttaaaagtga caaggtggga gctaaaattc ttcctttagc taaagcaatt gttaaccact
      301 ctttatccat taacatctac tttcacctgc ctctttctgc aaatccaggt ctaaaactcc
      361 ggcatcacta tagtacaaaa gacgttgagc acccctggct gtactttgcc gccgtgcttc
      421 tgcggcgcta acttctgcta ccgccagcgc agccgtagtg tgcgcagcac gtaatgcctg
      481 caccaccgct actgccgctg ctcgcccgcc tgcaccatag gccacatcaa caggcagtga
      541 caaagcaggc agttttttct gttttgttaa aactgtaatc aggtgatcga tactgacggc
      601 aaaaccaacc gctggttgag agggaccgaa attatctaac aactggtcat aacgtccacc
      661 accacagagg gcataaccaa gaccgcctgt atacccttca aaaaccatgc ctgtatagta
      721 gtctaataaa cgcaccaggc ccaaatctaa ttgcagatat ttagtgacgc catactcctc
      781 taacacagac caaacttcag ccagcgttgt taagggcagc gcagcctgcg gctccggtag
      841 tagttctcgg gccgccgtta agacctcccg cccaccacgt aagctaggta cacgtaagat
      901 attttccttc tgccacgcgg ctaagggcag ttgactaacc tggtttttta gcgctacaaa
      961 gtccttttta ttaaataagt gtttgatcaa agcacgctcg gtagtatcta cctgataagc
     1021 agaaagtaag ttttctaaaa agccactatg accaagacat aaggtaaatt ctgttaaacc
     1081 tgctgcttgt aacactgctg cagccaaagc aatgacttcg gcatcactgg cagcacttgg
     1141 ggaacccagc agctccacac ccgcctgcgt tagctcacgc tgcttaccct gctg
//
LOCUS       DASRMS010000196         4976 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_256715, whole genome shotgun sequence.
ACCESSION   DASRMS010000196 DASRMS010000000
VERSION     DASRMS010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 4976)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4976)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4976
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_256715"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            130..309
                     /locus_tag="VFC74_09270"
     CDS             130..309
                     /locus_tag="VFC74_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25556.1"
                     /translation="MFDKQETINRARALKEMCSKLRLYNCMGCPFFFEKMNGCVLADD
                     NLPRDWELDEVCEDD"
     gene            854..1264
                     /locus_tag="VFC74_09275"
     CDS             854..1264
                     /locus_tag="VFC74_09275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HZK25557.1"
                     /translation="MKGEDDKMNKVILKGRLAREIELKWTTSGKAVATSCIAVNRRGK
                     DAGADFINIVVWGTIAEVIAKYLAKGNEVLVEGRLQVRNYDDHDGNKRYVTEVVVETF
                     EFCGSKNGSTGNAGDSAASSFGGGTSGYNDDIPF"
     gene            1281..1970
                     /locus_tag="VFC74_09280"
     CDS             1281..1970
                     /locus_tag="VFC74_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25558.1"
                     /translation="MGKFSKKCKKNKAPQMPAFKKAEAKANSIDKETSDRVISEQDKL
                     NTSLTLVTMVHLNYIMHSEYGFGAKRFERLNSWIEWYSDAVKEKRFDYDSLEKEVVIA
                     VDPEIKGIGWKDYKPMRKVEPFGANQDMPKSYKELKDRMIIHKRRIFIEEVFTALELL
                     IATTLFDCYDWGLKRIERVITLLREARNWDIGKFFCILKYLSEHKCMDFKNRDIKDLM
                     ACRRNMGGLMA"
     gene            1937..2407
                     /locus_tag="VFC74_09285"
     CDS             1937..2407
                     /locus_tag="VFC74_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012047828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HZK25559.1"
                     /translation="MQKKYGRPDGVDMKKFRNKKTIVKGIEFDSKMEADYYRYLLGRL
                     QAGEILQIELQPKIMLQPGFKVAGQTVRAITYTPDFLVFYGDGRQEYIDVKGCQSQQG
                     NLRYKMYKYMYEKDGGVPLQWITASKKYSDSGWIDYFELNRIRRKTRKENIDAD"
     gene            2397..2732
                     /locus_tag="VFC74_09290"
     CDS             2397..2732
                     /locus_tag="VFC74_09290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25560.1"
                     /translation="MQIEIYPCICVAKDVVDKYGFEQLIVDLQQEIKEVEKAWLKMPS
                     SDDSALGKRFAEVGMELADVATRAVTMLHVMAEMQRCPSDNFIEDVFGWVAYKNKARG
                     YHDDSVNSF"
     gene            2743..2916
                     /locus_tag="VFC74_09295"
     CDS             2743..2916
                     /locus_tag="VFC74_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25561.1"
                     /translation="MKKMKLSQRYVWTEKAAKLNKNCKAGEELKGEREDWILKKMKEH
                     GIIKEIIMEVDNA"
     gene            2909..3355
                     /locus_tag="VFC74_09300"
     CDS             2909..3355
                     /locus_tag="VFC74_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25562.1"
                     /translation="MRKNGMRAWCYRYVDKLLFNEEKIRLEVESIRNDRHSERVGGGG
                     NAFISDPTATTALANIKPIDHVTIDGVVYRQPEEWLKIMAHVRSQCCDLELKVLEQRY
                     HKRARVGTTIDQLYISRATYFNIVDGIISYAVELGCQSGLLQVSRI"
     gene            3517..4773
                     /locus_tag="VFC74_09305"
     CDS             3517..4773
                     /locus_tag="VFC74_09305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013282964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HZK25563.1"
                     /translation="MFLTQSNVIRKLSKLEYSMLKEMCQYSNNLYNVALYNIRQYYFH
                     EKKFLTYENNYHECKTNENYKLLQAGVAQQILKVADRSFKSFLNLVKKAKNGEYRFKD
                     IKIPHYRGKGGLFNLILSTNAINIKDGYLIIPMSIEFSKSHYKHKIRIPFPKRLEGKN
                     IKEVRILPVNKGRHFKIQYCYEQPIEEQNLNQQNTIAIDIGLENLATCVTNKGTSFIM
                     DGRKLKSINQCWNKQKAYFQSIADKQKQKSTKRLQNLTRKRNNRTNDYIKKTARYIIN
                     YCIANNIGTVVCGYNGDFKRSMNLGKITNQQFTQISFGSLREALAGLCERYGMQYIEQ
                     EESYTSKASFLDLDEIPVYNPEHPYTEGFSGKRIKRGLYRSADGKVINADVNGACNIL
                     RKSKQNFDFEELCSGLLDSPLRIRVM"
BASE COUNT         1589 a          871 c         1277 g         1239 t
ORIGIN      
        1 aagaggcggt tgagtgctat tatgcgttga taagctattg caaagggcgt aaatgcgata
       61 gctgcccgtt ttggggtggt ggctttggat gtgggattaa tggtcctgca acgtgggagg
      121 gcggtcaagg tgtttgataa acaagagacg ataaatcgtg cgagagcatt gaaggaaatg
      181 tgcagcaagt tgaggctgta taattgcatg ggttgtccat ttttctttga aaaaatgaat
      241 gggtgtgtac tggcagatga taatcttcca agagactggg agctggacga ggtgtgtgaa
      301 gatgattagg tttgataaga taaagagcat ggaactggga gagctggctg attacttgga
      361 caagactctc aacgctccat gctcagtatg cgcttatgat gaggatgatt ggcattgttg
      421 taacaattgc aaaggttgtg ctgaaggtat caggctatgg ctggaggatg aagatagata
      481 aagataaact gattgcatac gcagatgcgt tacaggattt ttgcaaaacg agaggcggct
      541 ggtgcgtgta taatcagcct tcttcatggg attatggagg tgaacgatga gagatttaag
      601 ccgcattgat atggatcata cggagcttgt tgattcgcag ttacccttca gttggatatg
      661 cccgcattgc aggcaaagaa acataacgga taagtgtgcc aataatatac tgcttgaata
      721 cttaaaggtt atgcggcact gtaagcgttg cgggcatgta catctgtgga cgttgaagct
      781 gacggcacaa tttaaagaac gtgttgtgga ttatttaata aacggtggca tgtagtcaca
      841 gaggtgttta ttcatgaaag gagaggacga taaaatgaac aaagttatcc tgaaaggtag
      901 actggctaga gaaatcgaat tgaaatggac taccagcggc aaagccgtag cgacgtcttg
      961 catcgcagta aatcgtcgcg ggaaggatgc cggggcggat tttataaata tcgtcgtatg
     1021 ggggaccatt gctgaggtaa tagccaagta tctggcgaag gggaatgaag tgcttgtgga
     1081 aggccggctg caggtgcgca attatgatga tcatgacggg aataagcgct atgtcacaga
     1141 ggtggtagtt gaaacctttg agttctgcgg cagcaagaat ggcagcaccg gcaatgctgg
     1201 tgatagcgct gcttcttctt ttgggggcgg aacgtcaggc tataacgatg atataccgtt
     1261 ctgattagtg gaggagcaaa atggggaaat tcagtaaaaa gtgtaaaaag aataaggcac
     1321 cacaaatgcc agcgtttaaa aaagctgagg ctaaagcaaa ttctattgat aaagaaacgt
     1381 cggaccgtgt tattagtgag caagataagc tcaataccag tttgacgctg gttacgatgg
     1441 tgcatcttaa ctacataatg catagcgagt atggctttgg tgccaagaga tttgagaggc
     1501 ttaacagctg gatagagtgg tattctgatg cagttaaaga aaagagattt gattatgaca
     1561 gccttgaaaa ggaggttgtg atagccgtag acccggaaat aaaaggcatt ggctggaaag
     1621 actataagcc gatgagaaaa gtagaacctt ttggcgcgaa tcaggatatg ccgaaaagct
     1681 ataaggagct gaaggaccgt atgattattc acaagcgccg tattttcatt gaagaggttt
     1741 ttactgctct ggaattgctg attgctacga ccttgttcga ctgttatgac tgggggttga
     1801 agcgaataga gcgcgtaata acgctgctgc gcgaagcccg gaactgggat atagggaagt
     1861 ttttctgtat attgaagtac ctgtcagagc ataagtgcat ggattttaaa aaccgcgata
     1921 ttaaagacct gatggcgtgc agaagaaata tgggaggcct gatggcgtag atatgaaaaa
     1981 attcaggaac aaaaaaacta tagtaaaagg catagagttt gacagcaaaa tggaagctga
     2041 ttattatcgg tatcttctag gacggctgca ggcaggggag atactgcaaa tagagctgca
     2101 gccaaagata atgctgcagc caggtttcaa agtggctggc cagaccgtca gagctatcac
     2161 atatacacct gatttccttg ttttctatgg cgacggccgg caggagtata tagatgtcaa
     2221 gggctgccag agccagcagg gaaatctgcg ttacaagatg tacaaataca tgtatgagaa
     2281 ggatggaggc gtgccgcttc aatggatcac cgcatccaaa aaatatagcg atagcggctg
     2341 gattgattac ttcgagctta accggatacg caggaaaacc agaaaggaaa acatagatgc
     2401 agattgagat atatccatgc atatgcgtgg ccaaagatgt tgtggacaag tacgggtttg
     2461 aacagcttat tgtagacctg caacaagaaa taaaggaagt agaaaaggcg tggctgaaaa
     2521 tgccttccag tgatgattcg gccctcggaa aaaggtttgc cgaggtcggc atggagctgg
     2581 ctgacgttgc aacacgggct gtgactatgc tgcatgtaat ggcggaaatg cagcgctgcc
     2641 ccagcgataa ctttatagag gatgtgttcg gctgggtggc ttataagaac aaggcacgtg
     2701 gttatcatga cgattctgtt aatagctttt aggaggataa aaatgaaaaa gatgaagctg
     2761 tcccagagat atgtgtggac tgagaaagcg gccaagctga acaagaattg caaagctggt
     2821 gaggagctca agggcgagcg cgaggactgg atacttaaaa agatgaaaga gcacggaatt
     2881 atcaaggaaa tcatcatgga ggttgacaat gcgtaagaat ggcatgcggg cttggtgcta
     2941 ccggtatgta gataaactcc tgttcaacga ggagaagata aggcttgagg tcgagagcat
     3001 tcggaatgac aggcatagcg agcgtgtggg aggaggaggc aacgccttta tttccgaccc
     3061 taccgctacg acagcgctgg caaacatcaa gccaatagat catgtgacta ttgacggtgt
     3121 agtataccgc cagcctgagg aatggctgaa gattatggcg cacgtcagaa gccagtgctg
     3181 tgacctagag cttaaggttc tggaacagcg ctatcacaag cgtgccagag ttggaaccac
     3241 tattgaccag ctctacatca gcagggctac ttacttcaat atcgtcgacg gcatcataag
     3301 ctacgctgta gagcttggct gccagtcagg gctgctgcaa gtcagccgga tttaaaatga
     3361 aaaagcctag ggcttatacc ctaggcttta ctttatcata attggagaga agaccgcctc
     3421 ctctaagtga aacgaaggta gcggatgaat cgcctttaac ttgactattc acattacttg
     3481 ctatataata tctatataac aaaggaggtg aatgatatgt tcttaacaca atcaaatgta
     3541 atccgcaaac tatctaagtt agaatattct atgcttaaag aaatgtgcca gtacagtaac
     3601 aatctatata atgttgcttt gtacaatatc cgtcagtatt attttcacga gaaaaagttt
     3661 ttgacttatg aaaataacta tcatgaatgc aagaccaacg aaaattataa actgttacaa
     3721 gctggcgtgg ctcaacagat actaaaagta gctgatagaa gttttaagtc atttctcaac
     3781 ttagttaaaa aggcaaaaaa tggagagtac agattcaaag acattaagat tcctcattat
     3841 agggggaaag gcggtctttt taatctgata ctttcaacta atgccattaa cattaaagac
     3901 ggctatttaa taatacccat gagcatagaa ttttcaaagt cacactataa gcacaaaata
     3961 cggataccgt tcccaaaaag actggaaggc aagaacataa aagaagtacg tattcttcct
     4021 gtcaataaag gaagacactt caaaattcag tactgttacg aacagcctat agaggaacag
     4081 aatcttaatc agcagaatac tattgctatc gacattggat tagaaaacct tgctacctgt
     4141 gtaaccaata aagggacttc gttcattatg gacggacgta aactaaaatc aattaaccaa
     4201 tgttggaata agcagaaagc atattttcag agcattgccg ataaacaaaa acagaaatcc
     4261 actaagcgat tacagaatct tactcgtaaa cgtaataatc gtaccaatga ctacatcaag
     4321 aaaaccgctc gttacatcat taactactgt atagcaaata acattggtac agtagtatgt
     4381 ggatataacg gtgattttaa acgcagtatg aatttaggga aaatcactaa tcagcagttc
     4441 acgcagatta gctttggttc gttaagagaa gcattggcag gactttgtga aagatatgga
     4501 atgcagtaca tagaacagga agaaagctac acttcaaaag ccagcttttt agatttagat
     4561 gaaatacctg tctataatcc tgaacatcca tatactgaag gcttcagcgg aaaacgcatt
     4621 aagcgtggct tatatcgcag cgctgatggc aaagtaataa atgccgatgt aaatggtgct
     4681 tgtaatatac ttagaaaaag taagcagaac ttcgatttcg aagaactgtg tagtggactt
     4741 ttggacagtc cattaagaat aagggttatg taaccaatct tcttaagaat ctcccacttc
     4801 aaacgctgaa agcgttaagt ggtgagaatg ttcaactcct taactcaaat cgctatggcg
     4861 cacccggaaa tcaatgagtg tcaccaggta ctctggcggg attcgctctc ccagctccca
     4921 gttctggagc gtgcggtatg gcacgcccag aagcttgcac atttcaccgc gggaga
//
LOCUS       DASRMS010000197         1911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_391177, whole genome shotgun sequence.
ACCESSION   DASRMS010000197 DASRMS010000000
VERSION     DASRMS010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1911
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391177"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1911
                     /locus_tag="VFC74_09310"
     CDS             <1..>1911
                     /locus_tag="VFC74_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZK25564.1"
                     /translation="TEPSLKTLLERLGKLKAEAALASPLLYCLIDWVKKRLRNTDIRR
                     STAYQQLTSIGKSLLSTFDETELLSLTAEDFQAGYETMLENTPSAITRQNKADLLRRF
                     HAHASNILQLAPIKLSYADEGFFALPDANIITETEYQLLYQFLKSQKDTSLIAHAQLI
                     VLILGCRCGLRISEVRHLQLVDLQYPGMLEILSTERLEQLGGYAATLLIRGDQFNKLK
                     STSSRRQLPLNILMSSEERVELLNYHQAQRVKVGKRKVQGKFVFSEDNGKTPMDFNEL
                     QRSLHEQMRQLTGDPSLRFHHLRHSFASHALQASHNLTEPLQLPAHWKGHMLQGTSRK
                     HLHQISNYVGHSSGSVTLQHYTHNLDCVVRDLLWPHSFDHKKTGHGELQRYRGSLDVA
                     LCVQSLLDISADNLRQHNRQYDGKVLGWLPRRLKLKSAIDPNQWQPCSENLARLVTGT
                     SNEPRDLRDLNFENWCDFLERWSQLQTGDELIKMYKLDETNTRAQISLLSDLLKAQTR
                     FDNSRLKQPRKIFKTATTASSHYFIVRPPRYKLERDIAAQTYKNSLRLLQSPQNGDMA
                     KTALEYFYDNYRHWDKSVICRHDSSNLPKLQALEQFLVRVLPNDKVIVEQERNPAGHR
                     YPMIGKLQIVTV"
BASE COUNT          564 a          422 c          454 g          471 t
ORIGIN      
        1 cgacagagcc aagtttaaaa actttactag agcgtttggg caagttaaaa gcagaggctg
       61 cgctggcgag cccgttgctg tactgcctta ttgattgggt caaaaaacgc ctcaggaata
      121 cagatattag gcgtagtact gcctaccaac aattaaccag cattggaaaa agtcttctga
      181 gtacttttga tgaaacagaa ttgttatcac tgacagcaga ggattttcag gctggctacg
      241 aaaccatgct agaaaacaca ccatcagcaa ttacgcgaca aaataaagca gatttgttgc
      301 gcaggtttca tgctcacgca agcaatatcc tgcagttggc acccataaaa ctcagctacg
      361 cagatgaagg ttttttcgca ttaccggacg ccaacatcat cacagaaact gaataccaac
      421 tgctgtatca gtttctaaag tcacaaaaag acacatcact cattgcacac gcgcaattga
      481 ttgtgttgat tttaggttgc cggtgtggat tgcgtattag tgaggtaagg catttgcagc
      541 tagtcgatct gcagtatcct gggatgctgg aaatattatc tacagagcgg ttagaacagc
      601 tcggggggta tgccgcaact ttattaattc gcggtgatca gtttaacaaa ctaaaaagca
      661 cgagcagccg gcgtcagttg ccattaaaca tactcatgag cagcgaggag cgagttgaac
      721 tgctgaacta tcatcaggcc cagcgcgtta aagttggtaa aagaaaggtg caagggaagt
      781 ttgttttctc tgaggataat ggcaagacac caatggattt caatgagtta cagcgcagct
      841 tgcatgagca aatgcgtcag ttaacaggtg atccaagcct gcgatttcac cacttgcgcc
      901 atagctttgc cagccatgct ttacaggcga gtcataactt aactgagcca ttacaactgc
      961 ccgcgcattg gaaaggccat atgctgcagg gaaccagcag aaagcattta caccagattt
     1021 ccaactatgt tggccactcc agcggcagtg tgacattgca gcactacacg cataatctcg
     1081 attgtgttgt gcgagatctg ttgtggcctc acagttttga ccataaaaaa actggccatg
     1141 gtgagctgca gcgatataga ggctctctgg atgtcgcgct ctgcgtgcaa agcttgttag
     1201 atatcagcgc tgacaaccta cggcaacata atcgccaata cgacggaaag gttttaggct
     1261 ggctgccgcg gagacttaag ctaaagtctg ctattgatcc taaccagtgg caaccttgca
     1321 gtgaaaattt agcaaggttg gtaacgggca catcaaatga gcccagagat ctgcgtgact
     1381 taaacttcga gaattggtgt gattttcttg agcgctggag ccagttacag actggtgatg
     1441 agctgattaa aatgtataag ctggacgaga caaacacacg tgctcagatc tcactgcttt
     1501 cagatttact gaaggcgcag actcggtttg ataattcgcg gctcaaacag ccccgtaaga
     1561 tatttaagac agcaaccaca gcgtcaagcc attattttat agtgcggcca ccaaggtata
     1621 agctcgagcg cgatattgct gctcaaacct acaaaaacag tttacgtcta ctgcagtctc
     1681 ctcagaacgg agatatggct aaaactgcac tagagtattt ttatgacaat tatcgacact
     1741 gggataagag cgtcatttgc cgtcatgact caagcaacct gcctaaacta caagctctgg
     1801 agcagtttct agtcagggtg ttgcctaatg ataaagtcat agtagagcaa gaacgtaacc
     1861 ccgctggtca cagatatccg atgattggga agttgcagat agttacagta g
//
LOCUS       DASRMS010000198        28239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_993934, whole genome shotgun sequence.
ACCESSION   DASRMS010000198 DASRMS010000000
VERSION     DASRMS010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 28239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28239
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_993934"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            119..1738
                     /locus_tag="VFC74_09315"
     CDS             119..1738
                     /locus_tag="VFC74_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HZK25565.1"
                     /translation="MTKQSFVKGAAILTLAGLSVRGVGAVFRIVLAILITDEGVGLYQ
                     MAYPVYSTLLAISTAGIPIAISKLVAENVTYGNYRGAYRIFQTALFLLALFGLIVSLV
                     LYQGADYFARAIAMDERAYLPLISISPAIFLVSVMSAYRGFFQGQQQMIPTAFSQIAE
                     QLGRVVIGLVLVVLLLPRGLAYAAAGASFGAAAGALCGLCVLVVTWWQQRKGYLRHMQ
                     RQTAVDHQSFASIIKKIFSLAVPITLGSLVMPLISIVDLSVVPLRLRAAGFDISRATA
                     LYGQLAGMATPIIHIPTIITVSLAISLVPAISSALALHKTHLLQARAYLAMRLTLLLG
                     IPSAFGLYLLAEPICVLIFNNAEAGGVLAVSALGVIFLTLYQTTSAILQGLGRMMIPV
                     INLFWGALVKTGVTWLLTARPDFNVRGAALATVLGFGIAALLNVYQVQRLTGMPMRPL
                     ETIFKPLLASAVMGISVVACYGYAETMFMALQIAKANHIATIISVVVGAGLYLFCMLM
                     IGGITRDDLLMIPRLGPSLVTLAARFHILRG"
     gene            1744..3180
                     /gene="mazG"
                     /locus_tag="VFC74_09320"
     CDS             1744..3180
                     /gene="mazG"
                     /locus_tag="VFC74_09320"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HZK25566.1"
                     /translation="MKTIYIIGLGPGDPQAITLRSLAALKRIQHVYVRTARHPGLKVL
                     HDHQIAYKALDYFYQKSGTFAETYHQLAYFIMNAALRHDEVAYVVPGSPLFAEKAVEI
                     MLSKAPAAGITCRVLPAVSFLEALSADLKLPREQELVILDALQPDKLLNFPSRHVLIM
                     QAYNRQIASRVKLQLLSLYPDDHPITIVRGAGLKRGKRLVTVPLYKLDRLTWIDHLTT
                     IYVPPRSSHDIGDLLQVMRKLRGPHGCPWDQEQDHQSLKPYLLEEAYEVLAAIDHNDS
                     ENLCEELGDLLLQVVFHSEIAAEDNRFNFYDVVTGIVEKLIRRHPHVFAEVKVENTAE
                     VLQNWEEIKKAEKEEGQSLFALETYLPALLRAQKLQRKAASVGFDWPDAAGAWDKLQE
                     ELIELKDAYNTGDEAKIEEELGDFLFSTVNVARFLNLDAEQTLAKGVQKFFQRLRFVE
                     DQARATGKEMSAFPLAKLDEWWEMAKKQ"
     gene            3281..3553
                     /locus_tag="VFC74_09325"
     CDS             3281..3553
                     /locus_tag="VFC74_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HZK25567.1"
                     /translation="MNKSELIGVVAEKTGMTKKDIEKVVNAVFDGIGESLAQGDKVQI
                     IGFGTFDVRDRKEREGRNPATGETITIPAVKVPVFKAGKALKDNVK"
     gene            3751..4779
                     /locus_tag="VFC74_09330"
     CDS             3751..4779
                     /locus_tag="VFC74_09330"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HZK25568.1"
                     /translation="MYHKRVRLLGFLLIAVMICAGPGCAWLQRSPEQSPLPKQAAPNK
                     DTEQAPAPQAQIPKAISQGKKKEPQLRVYMAKKGAVETVQIEDYIQGVVAAEMDPTWP
                     QAALAAQAIIARSFTLQKIAENGGVPNRNAHASTDIKEFQAYDASRINDNVRKAVAAT
                     RGQAAVYGDEFIRGWFHAYAGPRTAMAKEGLEFKGANPPYIRIVDSPAEDVIPEEEKK
                     WQESFPLSRIRKVTREITGKDPGAVKKMEIAQKGPSGRVVRFRVNDVEVSGPQLRLAL
                     DSTVMRSTFVEELKISGDKVTMKGSGYGHGVGMCQWGARGLAEDGKSAEEIVKYYYRN
                     IEIVKLWE"
     gene            4926..5213
                     /gene="yabP"
                     /locus_tag="VFC74_09335"
     CDS             4926..5213
                     /gene="yabP"
                     /locus_tag="VFC74_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YabP"
                     /protein_id="HZK25569.1"
                     /translation="MDNYTRPQGNHRVVIDNRETLDVTGVVHVDSFDDEEVVLETEQG
                     LLAIRGEDLHIKKLNLEIGELAIEGLIIELAYSENKHLRDRGKNLFERLFK"
     gene            5237..5713
                     /gene="yabQ"
                     /locus_tag="VFC74_09340"
     CDS             5237..5713
                     /gene="yabQ"
                     /locus_tag="VFC74_09340"
                     /inference="COORDINATES: protein motif:HMM:TIGR02893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0034301 - endospore formation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="spore cortex biosynthesis protein YabQ"
                     /protein_id="HZK25570.1"
                     /translation="MQASFVTEQLITLAVTCGLGIVIGIGFDALSVLQQFMLLSKTAQ
                     FVIDLLYCLLMTVFVFIVLVLNSWGEVRAYTFFAMGAGGLFYYLFCHRFCRRQMVRGV
                     EVFIAVARFIGKPFIWSTRHLTRLVRSLSQLLKTVVQAGRHRWQKIKLKIHNKKKE"
     gene            5769..6134
                     /locus_tag="VFC74_09345"
     CDS             5769..6134
                     /locus_tag="VFC74_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HZK25571.1"
                     /translation="MRTVSVNKKLTYLPGAKAKAKPAVPVKNRATRVLLIIAVLYFTI
                     LLGSQYWRSVQLQHSLETIHTEIQTVKAQNEAMSKEIERLHSPAYLEQKAREDLGMVR
                     SGELLFYFQNKDKPSPQLP"
     gene            6195..6584
                     /locus_tag="VFC74_09350"
     CDS             6195..6584
                     /locus_tag="VFC74_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004264630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HZK25572.1"
                     /translation="MSLVVGAIVEGVVTGITHFGAFVQLANGETGLVHISEVADTYVK
                     DINEHLNKNDKVKVKILSVDGNGKIGLSIKKAAPSAGRGHHNRGPRVSFEDKLNKFLK
                     DSDERLQDLRRNTESKRGGRGSYKANM"
     gene            6716..7621
                     /locus_tag="VFC74_09355"
     CDS             6716..7621
                     /locus_tag="VFC74_09355"
                     /inference="COORDINATES: protein motif:HMM:NF014587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HZK25573.1"
                     /translation="MLIAAIDIGTNSVRMLLAATAAAQLTVVERTLTTTRLGAGLQNS
                     GYLSEAGREATLTAVKTNVQKARAQGADKIFILATSAMREASDGAAFARLLAQETACR
                     VEIISPAQEAEYSYTGVVKSLPELEQVLVFDLGGGSCEFIWHSQQQLHTVSYKIGALY
                     LTDRYLQHDPPQKKEIETAQAVIHENLHSLAPASKSLVGVGGTVTALTSLVLKLADYD
                     AKSMHGFCLTQEMVQAQLSKMLALPASERQKLVGMPSARAGILPAGALVIAELLTVMK
                     QPSLIVSEGDLLLGCLYQAISSTLI"
     gene            complement(7599..8039)
                     /locus_tag="VFC74_09360"
     CDS             complement(7599..8039)
                     /locus_tag="VFC74_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK25574.1"
                     /translation="MVLMALFLAFIAGIAMAVQGTLNTGLGKIIGLVESTFVVHVIGT
                     IVLVVLLFLLRLGRGELSRVTNVPWYLYLGGVISVLILYTVQAGISRLGVANATTAII
                     VGQVGAALLLDCLGCFGLEKIPFTWFKALGVALLAAGAKLMLMK"
     gene            8241..9983
                     /locus_tag="VFC74_09365"
     CDS             8241..9983
                     /locus_tag="VFC74_09365"
                     /inference="COORDINATES: protein motif:HMM:NF014474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HZK25575.1"
                     /translation="MKNVALIGNPNVGKSVLFYHLTGNYVVVSNYPGTTVEVSQGAAQ
                     LGGRTVTVYDTPGMYRLSPITEEEAVARRLLLERQMDCIVHVTDAKNLPRMLPLTLEL
                     LQYSYPVILVLNMMDEAEKLGVQIAQTELARRLGIPVIKTSLTQGQGLQALKRAVIEI
                     LKQGQLSKRTTTPPREESPKLLLQRHQAAAQLLQGVYLQGPAAAKSSWLEMLTLHPWG
                     GLLLMIVVLYAGLYLFVGRFGAGTLVNLIEGTLLGQIVLPALTHWLDNYLQWAPLRDL
                     VIGEFGLLTMGFRYAFGIILPIVSTFFFVFAILEDSGYLPRLAYWADRWLGKIGLNGR
                     AVIPLTLGLGCGTMAVLVTRTLESARERVLATFLLALAIPCSAQLGLILALLSAQPYY
                     LLVWVVVICAMFIFSGLLVHKILPGQRAPFFMELPPLRLPTLNAILQKTLARMRWYFM
                     EVIPVFIAVSVFLWLLQQTGVLTTLVALMQPYLGMLGLPSALSTVFLYGFFRRDYGTA
                     GLYDLYHAGLLNGAQLLLASIVLTLFLPCLAQLAMMIRERGVFVSALISFLVAVLAFI
                     GGVVVRIVLGLPTL"
     gene            9994..10227
                     /locus_tag="VFC74_09370"
     CDS             9994..10227
                     /locus_tag="VFC74_09370"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HZK25576.1"
                     /translation="MRNDTFLSELKVGEVGKIVELTTTDRKNIGKMMSLGIIPGVSVC
                     LLRRYPAFIVQIGYTKVALDHKLAVFIRVLPQP"
     gene            10329..12500
                     /locus_tag="VFC74_09375"
     CDS             10329..12500
                     /locus_tag="VFC74_09375"
                     /inference="COORDINATES: protein motif:HMM:NF039380.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HZK25577.1"
                     /translation="MSERTLAVEPYVRTSRAEGKISKKKFTWLKLAMPHELLCWETVG
                     AVSALVLLTQLQLMGELAPFALPFWAVASRNETRRMYLYGAVMLIAALLTEGVFYSVE
                     LLLGIVVYSLLWVRARKVSLPYVMVISIALLIGAIPRIWLAHFQPYEIFLLSLEIVLA
                     TLATVVFIQVFNRSPAYLCPERHIEGITAWIVFIGLLLLAMVQAAEVLAVVASGLAKF
                     VVLWASFWLGPGLAAAAGALLGFMLGIQGTSFAWVGVLTFAGFVAGLFRPYGRLATLL
                     AFSLGTGALALYVFGWESVVAEMLITLLASCIFLLTNIAPAKVKILAPFLRRGTTDEA
                     QKMRELTAVRVHDYGLVFRELADAFVQAAAVEEESRPALARLVEGVAARVCHNCASRR
                     RCWEKDVQRTYNAMLRVLADLDAGKSIIAMRTPEFFEKFCCKKDDFLRTVAFLHELEA
                     SGQAWQQKLQDSREVVTTQLMGLSQIMINLARDVREGLPEQQRIKNQYFHLELGIAQA
                     AKGDQEVCGDYYSYLELRDGKQAFILSDGMGNGKSAHQESQAAVKLVEQLLLAGFRKD
                     AVIRTVNTILQLRSPDEIFATLDVLIVDTEKGEAEFLKTGAAPSYLHTNKRVQEVKSA
                     SLPLGILSEVELKPVNLTLTEDSLIVMITDGVFEAAPGQPDWLKKYLVNQTYTHPQVL
                     ADDILVKARQLNGRAELRDDLTVLVCRAKRLKHKIRDYMTA"
     gene            12706..14100
                     /gene="tilS"
                     /locus_tag="VFC74_09380"
     CDS             12706..14100
                     /gene="tilS"
                     /locus_tag="VFC74_09380"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HZK25578.1"
                     /translation="MEKRVLETIKKRQLLTAGDKVLIAVSGGPDSLSLLHLLHKLAPE
                     LRIKLHLAHINHGLRSEAAAEALAIKKWALQMHLPVTVSRINVKSIQEKQHLSVEAAA
                     REGRYQALLAVAQQVGASRIATGHHRDDRVETLLLRLITGSGLDGLKGIPSRRSLAPG
                     IEVIRPLYDVPRKQIEAYCRQKKLQPLWDPSNAELVYLRNRVRLHLLPFLEREFGPHI
                     RRTLAQTTELLAADSSLIAELTAQAYATLAYTADNGTIVLKLDLLSALAEPLQARLVR
                     QALWQAGAKRIGHLHVQQVLAVMQSKSPSARCLVPGGIVVQREYKQLLVGWLKNKDSS
                     SPQSVSLQVPGRTYLPWSKEWLEAELIARSQVATLQVLPHEAYCAADKLQQPLTVRIR
                     EPGDRVKLLGSPGSRKVKKILIDKKIPQHKRDQLPVVIAGEEIIWLGGVDIAHAYRVT
                     ADTKQVLHLKITSM"
     gene            14114..14644
                     /gene="hpt"
                     /locus_tag="VFC74_09385"
     CDS             14114..14644
                     /gene="hpt"
                     /locus_tag="VFC74_09385"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HZK25579.1"
                     /translation="MSQKTVEKVLLSKEKIQQKIAELGAQISKDYAGRELLLVCVLKG
                     AVIFLADLMRQITVSVQIDFMAVSSYGAATESSGVVRILKDLDGSIENRHCLIVEDII
                     DTGLTLKYLKENLNSRQPASLKIVTLLDKPERRQVEIAPDYCGFQIPDEFVVGYGLDF
                     DEQYRSLPDICVLGDK"
     gene            14699..15319
                     /locus_tag="VFC74_09390"
     CDS             14699..15319
                     /locus_tag="VFC74_09390"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HZK25580.1"
                     /translation="MTAVILAGGKSSRMGQNKLFLPLGEQPVIGNLISRLQGKFAQLL
                     VVTDEPDLYGGFPVKVVSDEIICPVKNSLTGIHAGLKYAQTPYSLLVAGDMPFVHPAV
                     VDYLCRQAKNFDVIVPQEGHYFQPLCAVYHQNCISPIEQQLAQENYRIVGFFKYVRVQ
                     AIAMDKLMSLDPTGLTFFNINTPDDYQRAQSLLNCGIISEAKQKED"
     gene            15542..17464
                     /gene="ftsH"
                     /locus_tag="VFC74_09395"
     CDS             15542..17464
                     /gene="ftsH"
                     /locus_tag="VFC74_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HZK25581.1"
                     /translation="MFYIVILLLAMLMINFFNRPLDAPKKLSYDEFIASVDKGQVKEV
                     LIVNREISGTLADDTKFESYNLETDEVLTQRLLDKKNIKVSGEAPPEPGWWTSLASIV
                     IPFIILMVIFFVFMQQTQGGGNRVMNFGKSKARLHDSTRKRVTFDDVAGADEEKYELI
                     EIVEFLKEPRKFIELGARIPKGVLLVGPPGTGKTLLARAVAGEAGVPFFSISGSDFVE
                     MFVGVGASRVRDLFDTAKKNAPCIVFIDEIDAVGRQRGAGLGGGHDEREQTLNQLLVE
                     MDGFDAHEGTIIIAATNRPDILDPALLRPGRFDRQVAVSLPDVKGREAILRVHARNKP
                     LTNDVDLGVIARRTPGFSGADLENIINEGALLAGRRDRKLISMWELEEAIERVLGGTE
                     KRSRVISEFEKKIVAFHEAGHALVGYLLPNTDTVHKVSIIPRGRAGGYTLMLPEQDRY
                     YMTKTELLARITTLLGGRVAEELVLGDISTGAQNDLERATSIVRQMIMEYGMSDSLGP
                     ITLGKKHTEVFLGRDLARDRDYSEEIARSIDLEIRTLIDEQYKQAKELIIQNRTKLDL
                     LANALLEQETIDAEEIKALMEGKSLSDVPKRRRPGSQTEGQDVDGRNHRPSSQDSIIK
                     KDPLQVLINDKTIDIK"
     gene            17742..19505
                     /locus_tag="VFC74_09400"
     CDS             17742..19505
                     /locus_tag="VFC74_09400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014825206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HZK25582.1"
                     /translation="MVLDPTKHADWEIAEEAEKTMKTVQELGKELGLEQEELLPYGHY
                     VAKVDFAKVLKRLEDRPDGKYVDVTAITPTPLGEGKSTSTMGLTQGLGKRGKSVIATI
                     RQPSGGPTMNVKGSAAGGGLSQCIPLTPFSLGLTGDINAIMNAHNLAMVALTSRIQHE
                     FNSSDAFMERRGLKRLNIDPRNVEMKWIIDFCAQALRNITIGLGGKRDGFMMNSGFAI
                     AVSSEVMAILAVAKDLKDMRERMGRIVVAYDKHGNNVTTEDLGVAGAMTAWMVEALNP
                     NLLQTLEGQPVFVHAGPFANIAIGQSSIIADRVALKLSDYVITESGFAADIGFEKFWN
                     LKCRMSGLKPNAAVIVATIRALKAHGGAPLPLPGKPLDPAYSQEHLEWVEKGCENLVH
                     HVNTVKKSGINPVVCINAFHSDTDNEIKLVRRLAEEAGARVALSKHWQYGGEGALEFA
                     DAVVDACNEPNDFKYLYELDTPLRERINLIATEVYGADGVEYSAEAEAKLARMEKDPE
                     MKKLGTCMVKTHLSLTDNPMLKGVPKGWKLRVRDILTYNGAGFLVPVAGTISLMPGTA
                     ATPAFQRVDVNTETGKVQGLF"
     regulatory      19629..19721
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01831"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="THF riboswitches regulate genes encoding folate
                     transporters and folate metabolism; They are most commonly
                     found in Clostridiales and Lactobacillales; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="tetrahydrofolate"
                     /db_xref="RFAM:RF01831"
     gene            19798..20340
                     /locus_tag="VFC74_09405"
     CDS             19798..20340
                     /locus_tag="VFC74_09405"
                     /inference="COORDINATES: protein motif:HMM:NF018816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HZK25583.1"
                     /translation="MKRNYKLTTRDLTNAGLLVAMSIGLSRFAAVQIVSGTIRLSLGN
                     IPIYVAGLLFGPGIGGLVGGVADLSGYLVNPFGAAFVPQITLAAVMRGVIPPLVIRLF
                     GNKTRFWQLKIFLAIIVTELVAGAGLTTWGLAWLQKTPYFTVLTPRLLAMTVQVPIYA
                     MATYVLTVALRAINLQRHEG"
     gene            20346..20705
                     /locus_tag="VFC74_09410"
     CDS             20346..20705
                     /locus_tag="VFC74_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25584.1"
                     /translation="MEVTELKITNQSQAKKEIAKVGASTVSINIMAPKAVFRVIKITG
                     ISPIAANILKQEMLSKGGEAAVPAGAINCKEPSCDVIMMGTLQQFRKVIRSLRIQPLT
                     LPAIAELIKEQLFEAEA"
     gene            20936..21904
                     /locus_tag="VFC74_09415"
     CDS             20936..21904
                     /locus_tag="VFC74_09415"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HZK25585.1"
                     /translation="MKGKILRLLKENREKFLSGVSISRQLQVSRTAIWKYISVLRDDG
                     YRIKSVAGRGYQLQTVPDLLTPLEIDDGLRTKVLGQQIKYFQEIDSTNRVARKLAQAG
                     AADGLLVIAEKQKAGRGRLGRTWSSPSGGIWFSLILRPPLPPFKVQGLTLLTAVVAVE
                     TTALLCGLNVGIKWPNDLIVNGKKLAGILTEVSAEIERLHYVVVGLGLNANVSLSQLP
                     AEVETIATSILAETKRPLRRAEWIQTFLQRFEAAYQEAVVNGFAEILARWRRYSITLG
                     RTIVIHAGGKLITGRAVDINEQGALLVETKNGVETFWAGDVSLSND"
     gene            21922..22716
                     /locus_tag="VFC74_09420"
     CDS             21922..22716
                     /locus_tag="VFC74_09420"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HZK25586.1"
                     /translation="MLLAIDVGNTNMVLGIYQGTELKVSWRISTHRQQTGDEYGIILK
                     NLFSQTGITAEKLSGIIISSVVPPLMSALTEMSRRYMHQEPLVIGADINMGINLLVDN
                     PWEVGADRIVNAVAGYALYGGPLIIVDFGTATTIDAVSAAGDYLGGAIAPGIGISTEA
                     LFQRAAKLPRVELVRPQQVIGRNTIASMQAGIIYGFAGQVDGIVNRMAKEFTAAPQVI
                     ATGGLATLIATESETLQTVNPMLTLDGLRIIYERNAQQIYHVKEGS"
     gene            23104..24057
                     /locus_tag="VFC74_09425"
     CDS             23104..24057
                     /locus_tag="VFC74_09425"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HZK25587.1"
                     /translation="MHKKTVVLVLLLTLLCAAVGCRAKPAVPARSGKLTVYTSFYPLY
                     DFTRKIGGNHVNVINLVPAGASVHDWEPGPQTLASLLEADLLIVNGLGIEPWLNKLAA
                     TLEGNVPIVNTSAGVVLLYGSDDHVHNEHSGVADDEAAAAAVPDPHIWLDPANALQQA
                     ERIAAALIDLAPQYAEEFQDNLAAFRQAIAELDLAFQETLQTAARHEFVVTHLAFSYL
                     AKRYNLTQLAISGLTPQAEPSPAQMSELIKLMQEHNIRYIFQEPFTSGRLAAALAAEV
                     GAEILTLHPLAGLTPEEEKNGEDYFTIMRQNLAQLAKALRD"
     gene            24073..24837
                     /locus_tag="VFC74_09430"
     CDS             24073..24837
                     /locus_tag="VFC74_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HZK25588.1"
                     /translation="MINPVIQLEHVSFHYGPQMILEDVNLAVMPGEFLGIVGPNGSGK
                     STMLKIIVGLLQPNQGRVCLFGNERAKFKQYTRLGYVAQNATSFNHAFPATAYEIVLS
                     GLTPKLGILRQPGSEAKEQVEQALQQVGAEELAKQPIGDLSGGQQQRILIARALVAKP
                     ELLILDEPTVGVDLQALDQFYQLLLTLRQAHGLTILLVSHDLGLVSEHVTTLACLNKK
                     ICFHGPPADFWSTEIAAQVYGPAAKHVLPAHQGGLG"
     gene            24838..25695
                     /locus_tag="VFC74_09435"
     CDS             24838..25695
                     /locus_tag="VFC74_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HZK25589.1"
                     /translation="MDILRFAFMQRAFLAGIMVALLCPTIGIYLVLRRMSMMGNMFSH
                     LSLAGVALGIFTGIYPLATALTLSLAAAAAVEVLRRAYRIFSDLAIAILISAGTSLAV
                     ILISLSRAFNADLFSYLFGSVIAISTQDLYLIAAVGFLVFSIILMMQKELFYIAFDEV
                     GARAAGIRVDLVNMIFVAATSLTIAMAMRVVGMLLVSSLITVPIAAGLQLGRSFRATV
                     YWSYAIALISVVSGLVLAYYGDLAPGGTIVMCALALFVLAIFYGKMKRSGGKEHEQTA
                     TGAVEGKGL"
     gene            25652..26083
                     /locus_tag="VFC74_09440"
     CDS             25652..26083
                     /locus_tag="VFC74_09440"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HZK25590.1"
                     /translation="MSRQPLAQLKAKGYKLTQQRQQILEVLLTAPSRLSAQEVHSLLK
                     QQQVSISLDTVYRNLRLLVEIGAVQQISLQSGAVYELILQDELHYHVICVDCEKVVCI
                     PACSALQNCLQQAGAAGFTILNHSLEIYGRCSYCDKKSSGC"
     gene            complement(26119..27105)
                     /locus_tag="VFC74_09445"
     CDS             complement(26119..27105)
                     /locus_tag="VFC74_09445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HZK25591.1"
                     /translation="MKILHLNRFFYSGQTTHAFSLVREQQKQGLDARLIMDGNPSYQA
                     LALYKQTMEEIGAAIVKPGDLQASLNYLAQWRPQVIHAHSPLTYALAMTLARHFQASL
                     VITCHGLGINSREAQPFLLEAKALICISVRVANSMRQFQQKVHVIPNGVDLSEFKPGE
                     KTEPLKIAYVGRVDAGKQKGYSHFCKAVDLLEGVEFYVAADKKPNSKTAQYLGWTNEV
                     APLLAQTDIMAGTGRALIEGMAAGNAIIILGRNCQGILTPEKAAKEKFLDLSGLAGNE
                     PCYKNIFYDLAKLTQSKLYLEQLQTFGRDLAVKEYDNRKLTQRIIKIYRGVI"
     gene            complement(27221..27805)
                     /locus_tag="VFC74_09450"
     CDS             complement(27221..27805)
                     /locus_tag="VFC74_09450"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZK25592.1"
                     /translation="MKRLSMVLTVTLLVVLMTAGSVSATTLPPRWPQPAWFAQFQNAQ
                     PTPAPKLEPAPAPAPTPAINPTSLTLAEKTVFAGINRERTLRGLQPLTINYQLVKLAR
                     LKSKDMAENRYFAHRSPTYGSAYDMMRNNGIRYSYAGENLAKTSSAANAVKLFMGSSI
                     HRNALLNSRFNQTGIGVYQLGRQVYVTQMFIGVR"
     gene            complement(27956..>28239)
                     /locus_tag="VFC74_09455"
     CDS             complement(27956..>28239)
                     /locus_tag="VFC74_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25593.1"
                     /translation="SKEVQQKNHGLEQELQALQTSNAELKNTLSHTESLNSEQRKQLA
                     RLRNDLVAALNKEKEQSKKIALLETELENKKGVWGTLAGVFQNKTSPKK"
BASE COUNT         7629 a         5678 c         6998 g         7934 t
ORIGIN      
        1 ggtataatag taaaatatta tatattttgt gcattaattt gcctttagtg gttattttta
       61 cagtacatgt tataataaaa tgagattgca gggggaaaaa gctcaatggg gtgagaactt
      121 gaccaagcag tcttttgtta aaggagcagc tatcctaacg ttagcggggt tatcggtgcg
      181 tggggtgggt gcagttttta gaatagtttt agcgatactg attaccgatg agggtgtcgg
      241 cctctatcaa atggcctacc cagtctacag cacactacta gccatttcta cggcaggtat
      301 cccgattgct atttctaaac tggttgccga gaatgtgacg tacggcaatt atcgtggcgc
      361 ctataggatt ttccaaaccg cactattttt gctggccttg tttgggctaa ttgtttcgct
      421 tgtcctctat cagggagcag actattttgc ccgcgccatc gccatggatg aacgagcata
      481 tttgccctta atcagtatct caccggcaat ttttcttgtt tctgtgatgt ctgcttatcg
      541 tggttttttt caggggcagc agcaaatgat accgacagca ttttcacaga ttgccgaaca
      601 attagggcgg gtggtgattg gtttggtact agttgtgctg cttttgccgc gagggttagc
      661 atatgctgcc gcaggagcca gttttggtgc tgccgctggc gccttgtgtg gactttgtgt
      721 gctggtggta acctggtggc agcaaagaaa agggtatctg cgtcacatgc agcggcagac
      781 tgcagtagat catcagtcct tcgctagtat catcaaaaag atcttttcgc ttgctgtacc
      841 cattacactt ggtagtttgg tcatgccgct gatctccatt gttgatttat ctgttgtgcc
      901 gctgcgttta cgggcagctg gttttgacat cagtcgtgct accgccttat atgggcagtt
      961 agcggggatg gcgacgccta ttatccatat accaaccatc attacagtat ctctagctat
     1021 ctctttagtc cctgcaattt cttcagcctt agccttacat aaaacacact tactacaggc
     1081 gcgtgcgtat ttagccatgc gactaacttt gctcttaggc attccttctg cttttggcct
     1141 ttatctgctg gcagaaccaa tttgtgtttt aatattcaac aatgcggaag ctggcggtgt
     1201 cctggcagtt tcggctttgg gtgtgatatt tttaacgctc taccaaacta cttctgctat
     1261 cttgcaaggc ttgggaagaa tgatgattcc agtcattaat ctattttggg gcgcgctagt
     1321 gaaaaccggt gtgacgtggc tgcttactgc caggcctgat tttaatgtgc gcggtgcagc
     1381 tttagccact gtccttggtt tcggtatagc tgccttgctg aatgtttatc aggtgcagcg
     1441 attaacaggc atgcccatgc ggccgttaga aacaattttt aaaccgctct tggcgtctgc
     1501 tgtgatgggg attagtgtag ttgcgtgcta tggctatgct gaaaccatgt ttatggcgct
     1561 gcagatcgcc aaagctaacc acatagcgac aatcataagc gtggtagttg gggctggcct
     1621 ttatctcttc tgtatgttga tgatcggcgg tattaccagg gatgatctat taatgatccc
     1681 gaggctgggt ccttcgcttg tgacactggc ggcaagattt catattttaa ggggctgata
     1741 gatttgaaaa caatttatat tattggcttg gggccaggtg accctcaagc catcacctta
     1801 cgctcactag ctgcactgaa gcggatacaa catgtatatg tacggacagc ccgccaccca
     1861 ggcttaaaag tattgcatga tcaccaaatc gcttataagg cccttgatta tttttatcaa
     1921 aaatcgggga cctttgcaga aacataccac caactggcct attttattat gaatgccgcc
     1981 ttacgtcatg acgaagtagc atatgttgtg ccgggcagcc cactctttgc ggaaaaagca
     2041 gtggaaatta tgctctccaa ggccccggca gcaggaatca cctgtcgtgt gctgccagcc
     2101 gtttcatttt tagaggcctt gagtgcagat ttaaagctgc cacgtgaaca ggaactggtg
     2161 attttagatg ccctgcagcc ggacaagctg ttgaattttc ccagcagaca tgtattaatt
     2221 atgcaagcct acaatcgcca gattgcttct cgtgttaagc tgcagctgct ctcactctat
     2281 ccggacgatc atccaattac aatagtgcga ggtgcagggc taaagcgtgg caaacgccta
     2341 gttactgtgc cgctctataa attggatcgg ctaacctgga ttgatcacct aacgacaatc
     2401 tacgtgcccc cccgatcaag tcatgacatt ggcgatttat tgcaggtaat gcgcaagtta
     2461 aggggtcccc atggctgtcc ctgggatcaa gagcaggatc accaatctct caaaccctat
     2521 ttactggagg aagcatatga ggttctagca gcgattgacc acaacgacag cgaaaatctc
     2581 tgcgaagagt tgggcgattt attattgcaa gttgttttcc atagtgaaat tgcagcagaa
     2641 gataaccgct ttaactttta cgatgtggtc acaggcattg tcgagaaact aatccgccgc
     2701 cacccccacg tttttgcgga agtcaaagta gaaaatacag cagaagtact gcagaattgg
     2761 gaagagatca aaaaggcaga aaaagaggaa ggacaatcgc tcttcgccct ggaaacttat
     2821 ttgccggccc ttttgcgtgc ccaaaagctg cagcgtaaag ctgcctccgt aggctttgac
     2881 tggccagatg ctgccggtgc ttgggacaag ctccaggaag agctaataga gttaaaagat
     2941 gcatataata cgggggatga ggccaaaata gaggaagaac tgggtgattt cctcttttct
     3001 actgtcaatg tcgcacggtt tcttaattta gatgcagagc agacattagc taaaggtgta
     3061 caaaagtttt ttcagcgcct ccgcttcgtg gaggatcagg ctcgtgccac tggcaaggag
     3121 atgtcggcat tccccttagc aaagctggac gaatggtggg agatggcaaa aaagcaataa
     3181 aataaataaa aatagtgagt ttggcaggat ttatagatat tttaacgaat aagcaaagat
     3241 atcctcaaca ttaaaatttt ggtaacagga gggaaacgta gtgaacaaat cggaacttat
     3301 tggtgtagtt gcagaaaaaa ccggtatgac aaaaaaagac atagaaaagg tcgttaatgc
     3361 cgtttttgat ggtattggtg agagcttggc ccaaggggat aaagtacaaa ttattggctt
     3421 tggtactttt gatgtacggg accgtaagga gcgcgaaggt cgcaatccag ctacgggaga
     3481 aactattacc atcccagccg ttaaggtgcc tgtatttaag gcaggtaaag ctttaaaaga
     3541 taacgtaaaa taaattttta taaaaaggcg cgctcatttg gtttcctgat ggcgtgcttt
     3601 tttgtgccta aaaacgacaa tagttgtggg ctcagcagtg agctgattta gcagctaatt
     3661 aactaaagaa gcgctgcttt gccgaagagg atgtttaatt ttatgctttc tcccgaatac
     3721 tagaaaaaag atgattgcgg gaggttagct atgtatcaca aacgagtcag attgctggga
     3781 tttttattaa ttgcggtgat gatctgcgca ggacctggtt gtgcgtggtt acaacgttca
     3841 ccagaacagt caccgctgcc caaacaggca gcaccaaata aagacactga acaggctcca
     3901 gcgccacagg cccagattcc taaagccatt agtcaaggca agaaaaagga gccgcagctg
     3961 cgggtgtata tggccaaaaa gggagcggtg gaaacagtgc aaattgagga ttacatacag
     4021 ggcgttgttg ctgcagaaat ggatccaact tggccgcagg cggcattggc cgctcaagcc
     4081 attattgccc gcagctttac tctgcaaaaa atagcagaga atggtggtgt gcccaatcgt
     4141 aatgctcatg cttccactga tatcaaagaa ttccaggcct acgatgcgtc gcggattaat
     4201 gataatgtgc gcaaagcagt agcagcgacc cgtgggcagg cagccgtcta tggagatgaa
     4261 tttatcagag gttggtttca tgcttatgct ggtccgcgta cagcaatggc caaagaggga
     4321 ttagagttca agggggcaaa cccaccatac atccgtattg tggatagtcc ggcagaagat
     4381 gtgatccctg aagaagaaaa aaaatggcag gagagttttc ccttgtctcg tatacgaaag
     4441 gttacaaggg aaattacagg taaggatccc ggcgcagtaa agaagatgga aatcgctcaa
     4501 aagggtccct ccggacgggt agttcgcttt cgtgtcaatg atgtggaggt ttccggacca
     4561 cagctgcgtt tagccttaga tagcactgta atgcgttcta catttgttga ggagcttaaa
     4621 atttcaggcg ataaagtaac catgaagggc agcggatatg ggcatggtgt aggtatgtgc
     4681 caatggggag cacggggttt ggccgaagat ggcaagagtg cagaagaaat agtaaaatat
     4741 tattaccgca atatagagat tgttaaacta tgggagtaga tataaacggc caaacggccg
     4801 tttttttatg ctgtccgtaa aatgttcttg ataacatcaa gcgcctgctt ttttatttaa
     4861 aaagtcataa aattttgtcc ccttcataat atggaatata agtggaatca gggagggata
     4921 agcagatgga taattataca cggccgcagg gtaatcaccg agtggtaatt gataatcgtg
     4981 agacactaga tgtcactggt gtggtgcatg tagatagttt tgatgatgag gaggtagttt
     5041 tagaaacaga acagggcctt ttggcaattc ggggggaaga tttacatatt aaaaagttaa
     5101 acctggagat cggtgaattg gcaattgaag gtttaatcat tgaattggca tattcagaaa
     5161 acaaacatct tcgtgaccgg ggtaaaaacc tttttgagag gctgtttaaa taggcaacta
     5221 acggggagga gcacaagtgc aagctagctt tgtcacggaa caactgatca cactagctgt
     5281 tacctgcggt ctaggcatcg tgataggcat cggcttcgat gctctcagcg tgctgcagca
     5341 gtttatgctt ttatcaaaaa ctgctcagtt tgtcattgat ctgctttatt gtctgttgat
     5401 gacagtattt gtgtttatag tactagtatt aaatagctgg ggagaggttc gagcctatac
     5461 tttctttgct atgggtgcgg gaggactttt ttactatctg ttctgccacc gtttctgtcg
     5521 acgccagatg gtacgcggcg tggaggtctt tattgctgtc gctcgcttta ttggcaagcc
     5581 ctttatctgg agtacgcggc acttaactcg tctggttcgt agtttgtcgc agctgttaaa
     5641 aacagtggtg caggcaggca gacatcgctg gcaaaaaata aaattgaaaa ttcacaataa
     5701 aaagaaggaa taacaggtaa tggcagagaa tctaataata ctcaatgtcg cggtaagagg
     5761 ggatttttat gcgtacggtg tctgttaata aaaaacttac ctacctgcca ggggctaaag
     5821 ccaaagctaa acctgcagta cctgttaaga accgggctac gagagtactg ttaattattg
     5881 cggtgctcta ttttacgatt ctcctgggta gtcagtattg gcgttcggta caactgcagc
     5941 attctctgga gactattcat acagaaatac aaaccgtcaa ggcgcagaat gaagctatgt
     6001 ccaaagaaat agagcgtttg cattcaccag cttatttaga gcaaaaggca cgagaggatt
     6061 tagggatggt gcgttcaggt gaactactgt tctattttca gaataaggac aagccatcgc
     6121 cacagcttcc ttgacaaacg gtcaaaacca gatataatga gggtagataa tttttaaggg
     6181 ggaatttttt tcgtatgtct ttagtggtgg gcgccattgt tgaaggggtg gtcaccggta
     6241 tcacccattt tggagccttt gttcagctcg ccaacgggga aaccggcctt gtccacatct
     6301 ccgaagttgc tgatacctat gtgaaggata ttaatgagca cttaaacaaa aatgataagg
     6361 ttaaggtgaa aatcctctcc gttgatggaa atggaaagat tggtctttcc attaaaaaag
     6421 cggcaccaag tgcgggtcgc ggccaccaca acagaggtcc ccgagtttcg tttgaggaca
     6481 aactaaataa gtttcttaag gacagcgatg aacgtctgca agacttacgc cgcaatacag
     6541 aatcaaagcg tggcggcaga ggttcttata aagctaatat gtaaattaaa acagatatcc
     6601 tttggggata tctgtttttt gtactgcaga aattaaattg cttgatattt agtaagctgc
     6661 taatctaaac agatcatatc aagcaggaaa tgtatacttt ggggtgggtg atgtaatgct
     6721 gatagctgca attgatattg ggacaaactc tgtacgcatg ctgctggcag ctacagcagc
     6781 agcacagcta actgtcgtgg aaagaacact aacgacaacc agattaggtg ctggtttaca
     6841 aaatagcggc tatttatcag aggctggccg agaggccacc ttaactgcag tcaaaacaaa
     6901 cgtacaaaaa gctcgagcgc agggggcaga taagatcttt atcctggcaa ctagcgcgat
     6961 gcgtgaagcg agtgatggtg cggcctttgc acgtttgctt gcccaggaga cggcttgcag
     7021 agtagagatt attagtcctg cacaagaggc agaatatagt tatactggtg tcgtgaaaag
     7081 tttacctgag ctagaacaag ttttagtttt tgatttaggc ggcggttcct gtgaatttat
     7141 ttggcattcc cagcagcagc tccatacagt tagttataaa ataggggcat tgtacctgac
     7201 agatcgctac ttgcagcacg atcctccgca aaagaaagaa attgagacgg cacaagcagt
     7261 aatccatgag aatctgcact cattggcacc agcgtcaaag tcgctggtgg gtgtaggcgg
     7321 tactgtaaca gctttaacgt ctcttgtgtt aaagctggct gattatgatg ctaaaagtat
     7381 gcatggcttc tgtttaacac aggagatggt acaggcacag ctgtcaaaaa tgttagctct
     7441 gccggcctca gagcggcaaa agttagttgg catgccctca gcacgagcag gtattttgcc
     7501 ggcaggtgct ttagtgattg ctgaactact gacagtcatg aaacagccat ccctgattgt
     7561 ttcagagggt gatctcctgc tgggctgctt ataccaagct atttcatcaa cattaattta
     7621 gcaccggcgg ccagtaaagc gactcccagt gctttaaacc aagtaaaggg tattttctcc
     7681 agtccaaagc agcctaagca atctagcagc agtgctgcac ctacctgtcc cacaatgatg
     7741 gctgtcgtgg cgttggccac gcctagacgg gaaatgcctg cttgtactgt atatagaatt
     7801 aacacactga tgacaccgcc tagatataaa taccaaggca cattagtgac tctacttaat
     7861 tccccacgcc caagacgtaa gagaaaaaga agcactacca ggactattgt accaatgaca
     7921 tggacaacaa aagttgattc tactaaaccg attatttttc ctaaccctgt atttaatgtg
     7981 ccttgtacgg ccatagcaat accggcaata aaggctaaaa ataatgccat gagcaccata
     8041 cttcacggct ccttcccttc ttacatattt tttagtgtgt gctcgcaaag gatcctctat
     8101 gcgcaatact taggcagaga ggatcctttg tttttagtgc cagcgcaggg catgagtccc
     8161 ctcaaggaca agaaaaacac tttctaaaaa cgtattagtg tctttgctgc tgcatagaaa
     8221 ttagggaagg aggtgtgcag gtgaagaatg tagcattaat aggtaacccc aatgtgggca
     8281 agagtgttct tttctatcat ctcacaggca actacgttgt agtttctaat taccctggta
     8341 caacagttga agtttcacag ggtgcggctc aactgggggg ccgtacggtc acagtttacg
     8401 atacaccggg catgtaccgc ctttctccca ttactgaaga agaagctgtg gcacgtcgtt
     8461 tactgttaga gcgacagatg gactgtatcg tgcatgtgac agacgctaaa aatttaccca
     8521 gaatgctgcc gctgacacta gagttattac agtacagtta tccagtaatt ctggtcttaa
     8581 acatgatgga tgaagcagaa aaattaggtg tgcagattgc acagacagag ttagctaggc
     8641 ggctgggaat cccggttata aaaacttcat taacgcaggg gcagggactg caggcactaa
     8701 aaagagcagt gatcgagatc ttaaagcagg gacaacttag caaaagaacg acaactcccc
     8761 ctcgtgagga aagcccaaag ttactattgc agcggcatca agcggccgct cagctgttgc
     8821 agggggttta tctgcaaggt ccagctgctg ctaaaagcag ctggttagaa atgcttactc
     8881 tgcacccctg gggcggtctg ctgctaatga ttgtcgtact atatgcagga ctttatctat
     8941 ttgtcggtag gtttggcgcg ggtacactgg taaatttaat tgaaggtact ttattgggac
     9001 aaattgtttt acctgccctg acgcactggc tggacaatta cctgcagtgg gcaccgctgc
     9061 gagacttggt gatcggagag tttggtttac ttaccatggg ttttcgctac gcctttggta
     9121 ttattttacc tattgtcagt acctttttct ttgtttttgc cattttggag gatagtggtt
     9181 atctgcctcg tttagcttat tgggctgaca ggtggctggg aaagataggg ttaaacggcc
     9241 gcgcagtaat tcctttgacg ctaggtttag gctgtggtac catggctgtt ctagtgacga
     9301 ggactttaga aagtgcccga gaaagagtgt tggcaacctt tcttttagct ttagccattc
     9361 cttgttctgc gcagttaggc ttaatcctgg ctcttctttc agcacagcct tactacctgt
     9421 tggtttgggt agtagtaatc tgcgcgatgt ttatctttag tggcttacta gtacataaaa
     9481 tattgccagg ccaacgcgct cctttcttta tggagctgcc acctttacgg ctgcccacct
     9541 tgaatgccat tttgcaaaaa actttagctc gtatgcgttg gtatttcatg gaggtaatcc
     9601 ctgtttttat agcggtcagt gtatttttgt ggttgctgca gcaaacaggg gtgctgacaa
     9661 cactggtcgc gctgatgcag ccatatctag ggatgctggg tttgccttcc gctttgagta
     9721 cagtttttct gtatggtttt ttccgccgtg attatggcac agcaggttta tatgatcttt
     9781 accatgccgg cctcttaaac ggtgcacaat tgcttttagc ctccattgtg ctgacactat
     9841 ttttgccttg tttagcacag cttgccatga tgatacgtga gcggggggtc tttgtttcgg
     9901 cattaatcag ctttttggtt gccgtcttag cttttatcgg gggggtagta gtgcgcattg
     9961 ttttaggatt accgacactt taaggagaaa accatgagaa acgatacatt tttaagtgaa
    10021 ctaaaggttg gagaggtcgg gaaaattgtt gaactgacga ctactgaccg caaaaacata
    10081 ggtaaaatga tgagtctcgg catcatcccg ggggtttctg tgtgtttatt acggcgttat
    10141 ccagctttca ttgtgcaaat tggttataca aaagtagcgc tagaccataa gttggcagtg
    10201 tttattcgcg ttctgcccca gccctaattc ttgtcgaaaa attttttaaa gtaatcgccc
    10261 cgctctgaca aattcctgag gactaaagct ttataatctg aggaagcgaa tagaaagggg
    10321 ggatacccat gtcagagcga acgctggcgg ttgaacctta tgtgcgtaca agccgagcgg
    10381 aagggaaaat atcaaagaaa aaatttacat ggctgaagtt ggcgatgccc catgagctgc
    10441 tttgctggga aacggtaggg gctgtgtcag cactggtgtt actgacgcag ttacagctga
    10501 tgggtgagtt agcgcctttt gctttgcctt tctgggctgt agccagtcgt aatgaaacaa
    10561 gacgtatgta tctttatggt gcagtcatgc tcatcgcggc actgctgaca gaaggtgttt
    10621 tttactccgt tgaactcttg ctagggattg ttgtttacag tttactctgg gttcgtgctc
    10681 gcaaagtgtc tctgccttat gtgatggtga ttagtattgc cttgctgatt ggtgcaatac
    10741 cgcgaatttg gttggcacat ttccagccct atgagatctt tttgttaagt ttagaaattg
    10801 tactagccac tctggcgacg gtagtcttta ttcaggtttt caatagatca cctgcctatc
    10861 tttgtcctga acggcatatt gaagggatta ctgcctggat cgtctttatt ggtctgctct
    10921 tgttagccat ggtgcaagcg gcagaagtac tggctgttgt ggccagcggc cttgctaagt
    10981 ttgtagtcct ttgggcttct ttttggcttg gtcccggcct ggccgcggcg gctggagccc
    11041 ttttgggctt tatgcttggt atacaaggga caagttttgc ctgggtcggg gtacttacct
    11101 ttgcaggatt tgtggcaggc ttgtttcgtc cttacggacg gcttgctacg ctgttagctt
    11161 ttagtttagg cactggagct ttagccttat atgtttttgg ctgggaatct gtcgtggcag
    11221 aaatgttaat tactttgctt gctagctgca tttttttgct cacgaacatt gcacctgcta
    11281 aagtaaaaat tttagcacca tttctgcgac gcgggacaac tgatgaggcg caaaagatgc
    11341 gggaattgac agcagtgcgt gtacatgatt acggactagt gtttcgtgaa ttagcagatg
    11401 cctttgtcca agcagctgcc gtagaggagg aaagcagacc agctttagcg cggctggtag
    11461 aaggagtagc agcgcgcgtc tgtcacaatt gtgcctctcg acggcgttgt tgggagaagg
    11521 atgtacagcg tacctataat gctatgctgc gtgtgttagc agatttagat gccggaaagt
    11581 cgattatagc aatgcgcaca cctgaattct ttgaaaaatt ctgctgcaag aaagatgatt
    11641 ttttgcggac agtggctttc ctgcatgagt tggaagcaag tggacaagct tggcaacaaa
    11701 aactgcagga tagtcgtgag gtagtaacta cgcagttaat ggggttgtca caaattatga
    11761 ttaatttagc acgagatgta agggaaggtc taccagaaca acagcgcatc aaaaaccagt
    11821 attttcactt agaacttggc atcgctcagg cggccaaagg agaccaggaa gtctgcggcg
    11881 attattatag ctatctggaa ctgcgcgatg gcaagcaggc ctttatttta agtgatggta
    11941 tgggcaatgg taagagtgcg caccaggaaa gccaagctgc tgttaagtta gtagaacaat
    12001 tattgttagc tggttttcga aaagatgcgg tgattcgtac cgtgaacact attttgcagc
    12061 ttcgttctcc tgatgagatt tttgccactt tagatgtgtt gattgtggat acagaaaagg
    12121 gggaggcaga gttcttaaaa acaggagcgg caccaagtta cttacacaca aataagcgtg
    12181 tgcaggaagt taaaagcgct tccctgccct taggaatttt aagtgaggta gagttaaaac
    12241 ccgttaatct tacgctgaca gaagatagcc tgattgtgat gatcacagac ggggtgttcg
    12301 aggctgcacc gggacaaccg gattggctta aaaagtactt agttaaccag acatacacac
    12361 atccccaggt tttagcggat gacattttag tgaaagcacg tcaactgaat ggccgagccg
    12421 aactgcgaga tgatttaaca gttttagtgt gccgtgctaa acgtttaaag cataaaatca
    12481 gggattatat gacagcctaa atacgggtaa gcctatcccc tcgaccagag gggatatttt
    12541 ttcttcacta actggtagtg gatgtttaac aagtagtaaa ttcatcaact acactcaggc
    12601 aaacggtact ttaagcagga ttttagcacc gcatcaagaa gaataaaaaa gcttgcgctt
    12661 tcacttgctc tgggcaagct gtaactgtac taaggggcga tcgtcgtgga aaagcgcgta
    12721 ctggagacaa ttaaaaaacg acagctgctt actgctgggg ataaagtact aattgccgtt
    12781 tccggtggtc cagattcttt aagcttgctg catttattac ataagctggc acctgaactg
    12841 cgcattaagc tgcatcttgc ccacattaac catggcttac ggtcagaagc agctgcggag
    12901 gccctagcca taaaaaaatg ggccctgcaa atgcatttgc ccgttacagt gagcaggata
    12961 aatgtaaaaa gcattcaaga aaagcagcat ctttctgtcg aagcagcggc gcgtgaaggc
    13021 cgctaccagg cactattggc agtggcgcag caagttgggg cctcacgtat tgccactggg
    13081 caccacaggg atgatcgcgt ggaaacgtta ctgctaagac taattaccgg cagtggtctt
    13141 gatggactga aaggcatccc cagcagacgc tctttagccc cggggattga ggttattcga
    13201 cctttgtacg acgtgccccg gaagcaaatt gaggcctatt gccgccaaaa aaagttgcag
    13261 ccactgtggg atccttccaa cgctgaactg gtatatttac gtaatcgtgt tcgcttgcat
    13321 cttttgccct ttttagaacg agaatttggt ccccatatcc gacggacact ggcacagaca
    13381 acagaactgt tggctgcaga tagctcttta attgctgaac tgactgcaca ggcttatgcc
    13441 acacttgcct acaccgctga taatggtaca atagtgctca agctagattt attgtcagcc
    13501 ttggcagagc ccctgcaggc acgattagtt cggcaagcat tgtggcaagc tggagctaaa
    13561 aggattggtc atctacatgt ccaacaggtt ttggcggtga tgcaaagtaa atctccttcc
    13621 gctcgctgtc tggtgccagg tggtattgtc gtacagcgtg aatataaaca gttgctagtt
    13681 ggttggctca agaataagga cagctcttcg cctcagagtg taagtttgca ggtgccgggc
    13741 aggacctatc tgccttggag taaagagtgg ctagaggccg agctcattgc acgttcgcag
    13801 gtagctacgc tgcaagttct gccccatgaa gcatactgtg cggcggataa actacagcaa
    13861 ccgttaactg ttcggatacg ggaaccgggg gatagagtaa agctgttggg gagccccggc
    13921 agccgcaagg ttaaaaaaat cttgatcgat aagaaaatcc cccagcacaa gcgtgaccag
    13981 ctgccagtag ttattgctgg tgaggagatt atctggttgg gtggagtaga tattgcccat
    14041 gcttatcgtg taacagcaga tacaaaacag gtgttacatc taaagattac atcaatgtaa
    14101 aggatggtgc cttatgtcac aaaaaactgt ggaaaaagta ttattaagca aagaaaagat
    14161 tcagcaaaaa attgcagaat taggtgcaca gattagtaaa gactatgcgg ggcgggaatt
    14221 actgttagtt tgtgtactaa aaggtgcagt aatcttttta gctgatttaa tgcgccagat
    14281 tactgtatcc gtacaaattg atttcatggc tgtttcctcc tatggtgccg caactgagtc
    14341 ttcaggtgtg gtcagaattc ttaaggattt agacggcagt attgaaaatc ggcactgttt
    14401 aatcgtggaa gatattattg acaccggcct gacgctaaaa tatttaaaag aaaatctaaa
    14461 ctcccgtcag cctgcttcgc ttaaaattgt tactttactg gataaacctg aacggcggca
    14521 agtagagatt gctcctgact actgcggttt tcaaatccct gatgaatttg ttgtggggta
    14581 cgggttagat tttgatgaac aataccgttc tttaccagac atttgtgtac tcggtgataa
    14641 ataaaaataa ctgtggtact agtcatgaaa gtagtgaatt tatgtggaag caaaggatat
    14701 gacagccgtc atcctagcgg ggggcaaaag ctcccgcatg ggacaaaata agctattctt
    14761 gccgctaggt gagcaaccgg tcattggtaa tctcattagc aggttacagg gcaagtttgc
    14821 acagctgctg gtagtgacag atgagccaga cctttatggc ggcttccccg tgaaagtagt
    14881 gtctgatgaa attatttgtc ctgttaaaaa ttctttaacg ggtattcatg ccggcttaaa
    14941 gtatgcgcag acaccctact ctctgctagt cgctggagat atgccctttg tacatccagc
    15001 tgtggtcgat tatttgtgtc ggcaagcaaa aaattttgat gtcatcgtgc cacaggaagg
    15061 tcattatttt caaccattat gtgcagtcta tcatcaaaac tgtatttctc ccattgagca
    15121 gcagttagct caagaaaatt atcgcatcgt gggttttttt aaatatgttc gcgtgcaagc
    15181 catcgccatg gataaattaa tgagtttgga tcccaccggt cttacttttt ttaatattaa
    15241 cacgcctgat gattatcaaa gagcgcaaag tctgcttaac tgtggtataa tcagcgaagc
    15301 taaacaaaaa gaagattaaa gtaataaatt cgttagaatt aatttgcagc tataagatag
    15361 gatgtagtaa ggaaaaaata ttttctagct ggtttacgct cagtggttgc tttgctgtta
    15421 gtacaggcag taggtttgcg ccttgtcgct agggacataa tatgttacaa ttaacaaatg
    15481 aggtaaactg ggcctttgaa gagaggaggc agtttttttg aatcgatttt tccggcaggc
    15541 aatgttttat attgtgatac tgctgctagc tatgctcatg attaacttct tcaatagacc
    15601 attagatgca cccaagaagt tgtcatatga tgagtttatt gcttcggttg ataaagggca
    15661 ggtaaaagag gttttaattg ttaatcgtga aatttctggg acgttagcag atgataccaa
    15721 gttcgaatct tataatttag aaactgatga ggttttaaca caacgtctgt tagataaaaa
    15781 gaacattaaa gtgagtgggg aagcaccacc agagccaggt tggtggacca gtctggcctc
    15841 gattgtcatt ccctttatta ttttaatggt gattttcttt gtctttatgc agcaaacaca
    15901 gggcggcggt aaccgggtga tgaattttgg taaatcaaaa gcccgtttac atgatagtac
    15961 gcgaaaacgt gtgacttttg atgatgtcgc tggcgctgat gaggaaaagt atgagcttat
    16021 agaaattgta gagttcctca aagaaccgcg taaatttatt gagctgggcg cacgtattcc
    16081 taaaggggta ctgttggtag ggcctccggg aacaggtaaa acgttactgg ctcgtgctgt
    16141 tgcgggtgag gctggtgtgc ctttctttag tatttccggt tcggattttg tggaaatgtt
    16201 tgtcggcgtt ggagcctccc gggtgcgtga tttatttgac acagccaaaa agaatgctcc
    16261 ctgcattgtt tttattgatg aaattgacgc cgtagggcgt cagcgtggtg ctggtttggg
    16321 cggcggtcac gatgaacggg agcaaacctt gaatcaatta ctggtagaga tggatggctt
    16381 tgatgcccat gaagggacga ttatcattgc tgccacaaac cgtccagata ttttagaccc
    16441 ggcattactt cgtccaggac gctttgatcg tcaggttgca gtaagtctac cggatgttaa
    16501 aggccgggaa gctattctca gagtacatgc tcgcaataag cctttaacta atgatgtgga
    16561 tttaggggtg attgcgagaa gaacccctgg ttttagtggt gctgacttag aaaatatcat
    16621 caacgaaggg gcactattgg caggccgtcg tgatagaaaa ttaattagta tgtgggaact
    16681 ggaagaagca attgagcgtg tccttggtgg taccgaaaag agaagccgcg taattagtga
    16741 attcgagaaa aaaatagtgg cctttcatga ggcagggcat gctttggtag gttatcttct
    16801 gccgaatact gacactgtgc ataaggtttc cattattcct cggggtcgag ctgggggcta
    16861 tactttgatg ttacccgaac aggatcgtta ctatatgacg aaaacagagc tgttggctcg
    16921 catcactaca ttactgggtg gcagggtagc agaagaatta gtacttggtg atatttccac
    16981 aggagcacag aacgacttgg agcgtgccac ttccattgtt cgtcaaatga tcatggaata
    17041 cggcatgagc gacagtttag gccccattac tttgggcaaa aaacatactg aagtattttt
    17101 agggcgtgac ctggctcgtg accgtgatta ttcggaagaa atcgcccgtt ctattgactt
    17161 agaaattcgc acgctgattg atgagcagta taagcaggca aaagagctga ttatacaaaa
    17221 caggactaaa ttggatttat tggcaaatgc cttactagag caggaaacta ttgatgctga
    17281 ggaaataaag gcactgatgg aaggtaaatc gctgagtgat gtaccaaagc ggcgcagacc
    17341 aggaagtcaa actgaagggc aggatgtaga tggcaggaat caccggccat ccagtcagga
    17401 tagcatcata aaaaaggacc ctttacaagt gttaattaat gacaaaacga ttgatattaa
    17461 atgactataa acagcactaa aaaaaacacg gattttccaa tccgtgtttt ttcttgtttg
    17521 gtaaaaggaa gtcaagctat tgaagtcaag ctatttatgt ttgatgatag aatacttttt
    17581 tgatcttagt ttgataataa cagcgacatg ttgttatcac aatattttat gggacatttg
    17641 tgaaaagttg ttctatgtag gcaggaataa ctaaatatgt gtagaaatga aactagtggc
    17701 acaagtaatt agttaaaatc atagtaggga gggatttttt aatggtactt gaccccacaa
    17761 agcatgctga ctgggaaatt gccgaagaag cagaaaaaac catgaaaaca gttcaagaat
    17821 taggtaaaga attgggactg gaacaggaag aactgctgcc ttacggccac tatgtagcga
    17881 aagtagattt tgctaaagtt ctgaaacgtc tagaagatcg tcctgatggt aaatacgttg
    17941 acgtaactgc catcacacca acccctcttg gtgaaggtaa atcaacttcc accatgggtt
    18001 taacccaagg gcttggtaaa cgcggtaaaa gcgtaattgc tactattaga cagccatctg
    18061 gcggaccaac catgaacgta aaaggttctg ccgctggcgg cggattatca caatgtattc
    18121 cgctaactcc attctcttta ggcttaactg gtgacattaa cgccattatg aatgctcata
    18181 atctggccat ggtcgctctt acttctcgta ttcagcacga atttaactct agcgatgcct
    18241 tcatggaaag aagaggcctg aagcgcttaa acattgatcc tcggaatgtg gaaatgaagt
    18301 ggattatcga cttctgtgcc caagctctgc gtaacattac tattggtctt ggcggtaaga
    18361 gagacggttt tatgatgaat tctggttttg ccattgccgt gtcctccgaa gttatggcta
    18421 ttttggctgt agctaaagac ttaaaagata tgcgtgaacg tatggggcgc atcgtggtag
    18481 cctatgataa gcatggcaat aatgtgacca ccgaagatct cggtgttgct ggcgctatga
    18541 ccgcctggat ggttgaagca ctgaatccca acttgctgca aactttggaa ggtcagcctg
    18601 tatttgtaca tgctggtcct ttcgccaaca ttgctattgg ccaatcttcc attattgctg
    18661 accgtgtggc acttaaactt agtgactatg taattacaga atccggtttt gctgctgaca
    18721 tcggtttcga gaaattctgg aacctgaagt gccgtatgtc aggcttaaag cccaacgctg
    18781 ctgttattgt agcaaccatt cgtgctctga aagctcacgg tggagctcca ctgccactac
    18841 ctggcaaacc tttagatcca gcatactcac aagagcatct tgagtgggtg gaaaaaggtt
    18901 gtgaaaacct cgttcaccac gttaacacag ttaagaaatc tggcattaac ccagttgtct
    18961 gcattaatgc tttccattca gatactgata acgaaatcaa gcttgtacgt cgtctggctg
    19021 aagaagctgg tgcacgtgtt gccctttcca aacattggca gtatggcggc gaaggtgccc
    19081 ttgaattcgc cgatgctgta gttgacgctt gtaacgagcc caatgacttt aaatatctct
    19141 atgagctcga taccccacta cgcgaacgca ttaatctaat tgccacagaa gtttacggtg
    19201 ctgatggtgt ggaatacagc gctgaagccg aagcaaaact agctcgtatg gaaaaagatc
    19261 cagaaatgaa aaaattaggt acctgcatgg ttaagactca cttatcctta acagataacc
    19321 ccatgctcaa aggtgttcct aaaggctgga aactacgtgt tcgtgacatc ttgacttaca
    19381 atggcgctgg cttcttagtt ccggtagcag gtaccatttc cctgatgcct ggtacagcag
    19441 ctacacctgc tttccaacgt gtagatgtta acaccgaaac tggtaaagtt cagggcttat
    19501 tctaaaaaat aagactggtt atttcaagtc ggctatggta aactgtagtt gcacaaataa
    19561 ataaaaaagc ggtttttact acatcatgga tttgtaccga attcgtaaaa gaactcggag
    19621 caaatatttg cagagtaggt gacatgcgtc aagtgctatg ggatgggaag ttgcctgtag
    19681 acgaaaggtc gcgcctgcga tatgtcaccg catccgctgt cgcactgaag agtaaggaaa
    19741 cctctttctc ttaggtgtgg cactctctgc catcctaagg gaaggaggtg tttttttgtg
    19801 aagcgaaatt ataagcttac cacaagggac cttactaatg ctgggctcct ggtagcgatg
    19861 agtatcgggc tcagccgttt tgctgctgtg caaattgttt caggtacaat tcgtctttct
    19921 ctcggtaaca ttcctattta tgtagctggt ttattatttg gccccggaat cggggggttg
    19981 gttggtggag tagctgattt atccggctac ctagttaacc ctttcggtgc cgcctttgtt
    20041 ccacagataa ctctagccgc agtgatgcgt ggtgtgatcc ctccacttgt gatccgttta
    20101 tttggcaata agacacgttt ctggcagcta aaaatatttc tcgcaattat tgtgacagag
    20161 cttgttgccg gtgcaggtct gacgacttgg ggactagctt ggctgcagaa gacaccatat
    20221 tttactgtac taactcctag actgcttgcc atgacggtgc aagttcctat ttatgccatg
    20281 gctacttatg tattaactgt tgcgctccgc gccataaact tgcaaagaca tgaggggtga
    20341 tagttgtgga ggttacagaa ttaaagatta ccaatcaatc gcaggccaaa aaagaaattg
    20401 ccaaagtagg tgccagcacg gttagtataa atattatggc tcctaaagcc gtttttcgcg
    20461 tcattaagat aaccggtatt tcaccgatcg cagctaatat tcttaaacaa gaaatgcttt
    20521 ccaaaggcgg tgaagctgca gtaccagctg gcgccataaa ttgcaaagaa ccaagttgtg
    20581 atgtgattat gatggggaca ctacagcaat ttcgcaaggt aattcgcagc ttgcgcattc
    20641 aaccccttac tctgccggcg atagccgaat taattaaaga gcagttattt gaggcagaag
    20701 cttaatagta aaacaatgta aacagcccgg gttttccggg ctgtttcttt atagtagatc
    20761 cgagatggca gatagctggc agtgaaagac cactaggcgt aaccttcaac tgactgggcg
    20821 gggaaattta gcgtgatcta ggtgtgtact gcagacgcag acttttcatc tgttaaattg
    20881 acaagctgct tagcactctt tacaatgaag gggtaagaaa atcagggagg ccatcatgaa
    20941 ggggaaaatt ttgcgtttat taaaggaaaa tagggagaag tttctctccg gtgtaagtat
    21001 cagccggcaa ctgcaagtaa gccgtaccgc tatttggaag tatattagtg tcctgcgtga
    21061 cgatggttat cgtattaagt cggtggcggg ccgtggttac caattacaaa ctgtgccaga
    21121 tctactaacc ccactggaaa tcgacgatgg cttacggaca aaggtgctgg gacaacagat
    21181 caaatatttt caggagattg actccactaa ccgtgtggcc cgaaaattgg cgcaagctgg
    21241 agctgctgat ggtttactgg taattgccga gaaacaaaaa gcaggcagag gacgtttagg
    21301 cagaacatgg agttcaccta gtgggggaat ttggttttca ctaattttgc gaccaccctt
    21361 accgcccttt aaagtacaag ggctgacttt attgactgca gtggtggctg tggagacaac
    21421 tgctttgctt tgcggtttga atgtaggcat aaaatggccc aatgatctaa ttgttaatgg
    21481 taagaaactg gcaggtattt taacagaagt atcagcagag atagaacgac tgcattatgt
    21541 agtggtaggc ctaggcctca acgctaatgt ctctttgtct cagttgccag ctgaagtaga
    21601 aaccattgcc acatctattt tggcggaaac aaaacgtccg cttaggcgtg cagagtggat
    21661 acagactttt ttgcagcgtt ttgaagcggc atatcaggaa gccgtagtaa atggtttcgc
    21721 agaaatacta gcacgctggc ggagatattc gatcacgctg gggcgaacga ttgtgataca
    21781 tgctgggggc aaactcataa ctggtcgagc cgtagatatt aacgagcaag gtgccttact
    21841 ggtggaaaca aaaaatggcg tggagacttt ctgggctgga gatgtttcgc tgagcaatga
    21901 ttaattaagg gggaataaaa tgtgctgtta gctattgatg tgggcaatac caatatggta
    21961 ctgggtattt accaagggac agaacttaaa gtatcttggc gaatttctac tcatcgtcaa
    22021 caaactggag atgagtacgg cattatcctg aaaaacctat tttcgcagac aggcatcacg
    22081 gcagagaaat taagcgggat tattatttcc tctgtagtgc cgccgctaat gtcggcatta
    22141 acggaaatgt cacgacgcta catgcaccag gagcccctgg taattggtgc agacataaat
    22201 atgggcataa acttgctagt ggacaaccct tgggaggtag gagcagaccg gatagttaat
    22261 gctgttgccg gttacgcgct atatggtgga cctctaatta tcgtggactt tggtacagcg
    22321 accaccattg atgcggtctc cgctgctggt gactatttag gaggagccat tgcgccagga
    22381 attggtattt ccacggaagc actctttcag cgagcagcaa aactgcccag agtggaactg
    22441 gttagaccgc agcaagtaat tggtcgcaat acgattgcga gcatgcaagc gggaattatt
    22501 tatggctttg ccgggcaggt tgacgggatt gtcaaccgca tggccaaaga atttacagca
    22561 gcgccccagg ttatcgccac aggtggtttg gcaactctaa ttgccacgga aagtgaaact
    22621 ttgcagacgg ttaatcccat gttaacttta gatggcttgc ggattatcta tgagagaaat
    22681 gctcagcaaa tttaccatgt aaaagaggga agttaaacgt gcctgtagaa atagtagtta
    22741 gatgtaatga ggggggtatg ggagaatgcc ctataaaatc tgtccgtttt gtggtaaagt
    22801 ttcttactct gctgcagaat cgaagacaat tgaatggcgt tgcccttact gcaatgaaga
    22861 tatctcacat ataatcgggc ttacttcttt accaaaagat aaagaagtaa agtaaactgt
    22921 ggataggtta aaactaatct gcagtttttt ttcgagaaga attgctgtga cgcgagcaca
    22981 aaaaatgact agcatcaaag gcagcaggca ggcctttttt ttatttgttt attggtgact
    23041 gaacttgtat aattaagtgt aaataagaaa attactactt ggtaaaagta agcggaggtt
    23101 gccatgcata aaaaaactgt cgttctagta cttttgttaa ccttactctg tgctgcggtg
    23161 ggctgtagag ctaaaccagc agtgcctgcg cgttcaggta aacttacggt ctatacttct
    23221 ttttacccac tgtacgactt tacacggaaa attggtggta atcatgttaa tgtcatcaat
    23281 ctggtgcccg cgggggccag cgttcatgat tgggagcctg ggccgcaaac gctggcttct
    23341 ttactagaag ccgatttgtt aattgtcaat ggtctcggca tcgagccctg gttaaataag
    23401 ctagcagcta cactcgaagg aaacgtgcct attgttaata cttcagctgg tgtcgtcctg
    23461 ctgtacggct ctgatgatca tgtgcacaat gagcatagcg gtgtggctga cgatgaggcg
    23521 gccgcagctg cagtacctga cccccatatt tggttagatc ctgccaacgc cttacaacag
    23581 gctgaaagaa ttgccgcagc tctgatagat ttagcgccgc agtatgcgga agaattccag
    23641 gataatttgg cggcttttcg tcaggccata gcagagttgg atttagcttt ccaagagact
    23701 ttacaaacgg cagcacggca tgaatttgtg gtcacgcatt tagctttcag ttatttagca
    23761 aagcgctaca atttaaccca gctggctatt agtggtctta ctccacaagc ggagcctagt
    23821 ccagctcaaa tgagcgagtt aatcaaactg atgcaagaac ataacatccg ctatatcttt
    23881 caggagcctt ttacctcggg tcgtttagca gcagcacttg ctgcagaggt aggggcggaa
    23941 attttaacac tccatcccct agcaggttta acgccagagg aagaaaaaaa cggcgaagat
    24001 tactttacaa ttatgcggca aaacctggct caactagcta aagcattgag agattaaggg
    24061 aggactgaac aaatgattaa tccagtgata caactagagc atgtatcctt tcactatgga
    24121 ccacaaatga ttctcgagga tgttaattta gcagtaatgc caggtgaatt tttaggtatc
    24181 gtaggtccca atggttcagg aaaaagtacg atgctgaaaa taattgtggg tttgctgcag
    24241 cctaatcagg ggcgggtatg cctttttggt aacgagcgag ccaaatttaa acagtatacg
    24301 cgtcttggtt atgtagcaca aaatgctact tcctttaatc atgcctttcc tgcaacggct
    24361 tatgaaattg tgttgtccgg cttaacacca aaactaggta ttttaaggca gcctggttca
    24421 gaagcaaagg agcaggtgga gcaggcgtta cagcaagttg gcgcagagga gctggcaaaa
    24481 cagccaatcg gcgacctctc tggagggcag cagcaacgca ttttaatagc gcgcgcttta
    24541 gtagctaagc ccgagctatt aatcttagat gaaccaacgg taggcgttga tttacaggca
    24601 cttgaccaat tttatcagct tcttcttacg ctgcggcaag cgcatggttt aacaatttta
    24661 ctggttagtc atgatcttgg tctggttagt gaacatgtta cgacgttagc ttgcctcaat
    24721 aaaaaaatat gttttcatgg cccgccagca gatttttggt caacagagat agctgctcaa
    24781 gtgtatggtc cggccgctaa acatgtatta ccggcacatc aaggagggtt aggttaattg
    24841 gatattttac gcttcgcctt tatgcaaaga gcttttttgg caggtattat ggtagcattg
    24901 ctttgcccaa ccataggtat ttatttagtt ttacgcagaa tgtccatgat gggtaatatg
    24961 ttctcacatc tatcgttagc tggtgtcgcg ctaggtatat ttacaggtat ctatcccttg
    25021 gcaacagctc ttactttatc tttagcggct gccgctgccg tggaagtact gaggcgagcc
    25081 tatcgcatct ttagtgactt ggcgattgct attcttattt ctgcaggcac tagtttagcc
    25141 gttattttaa ttagcttgtc acgcgccttt aatgctgact tattttctta cctttttggc
    25201 agtgtcattg ctatttccac acaagatctt tatctgatag cagcggtagg cttcctggtc
    25261 ttcagtataa ttttaatgat gcaaaaagaa ctcttttata ttgcctttga tgaagtaggg
    25321 gcaagggcgg caggaatccg agtagactta gtgaatatga tctttgtagc agctacttct
    25381 ttgaccatag ccatggcaat gcgggtggtg ggcatgctgt tagtctcctc tttgattact
    25441 gtgcctattg ctgccggcct acaattgggg agaagttttc gggccacagt atactggtct
    25501 tatgccatag cgcttatttc cgtcgtttcg ggtcttgtgc tggcctacta tggcgatttg
    25561 gccccgggtg gtaccattgt gatgtgtgca ctagctttat ttgtcttagc gatattctat
    25621 ggcaagatga aaagaagtgg aggtaaagag catgagcaga cagccactgg cgcagttgaa
    25681 ggcaaagggt tataagttga cacagcaaag gcagcagatt ttagaagtgc tgcttaccgc
    25741 tccttcccgt cttagcgccc aagaagtcca tagcttgtta aagcaacaac aagtatctat
    25801 tagtcttgat actgtttatc gtaatttacg gttactggtg gagattggtg ccgtacaaca
    25861 aatatctttg caatctggtg cagtttatga attaattctg caggatgaac ttcattacca
    25921 tgttatttgt gtagattgtg aaaaagtagt ttgtattcct gcctgctctg cgttgcagaa
    25981 ttgtttacag caggcaggag cggcaggatt tactatcctt aatcatagtt tggagattta
    26041 tgggcgctgt tcatactgcg ataaaaaaag ttccggctgc taaaacagcc ggaacttttt
    26101 cgtttggttt tacgacggct aaattacgcc tcgatagatc ttaataatgc gctgagttaa
    26161 ctttctatta tcgtattctt tgaccgccaa gtcacgccca aaggtctgca gttgttctaa
    26221 atataatttg ctttgggtga gctttgcgag gtcgtagaaa atgtttttat aacagggctc
    26281 attgcccgct agaccactaa ggtctaaaaa cttttcttta gcagcttttt ctggggtcaa
    26341 gatgccctgg caattgcgac cgagaatgat gatagcattt ccagcggcca tgccctcaat
    26401 cagagcacgg ccggtgccag ccataatatc tgtttgcgcc agtaagggag caacttcatt
    26461 agtccaaccc agatactgtg cagttttgct gttgggcttt ttgtccgctg ctacgtaaaa
    26521 ctctaccccc tctaagagat cgactgcttt acaaaaatgg ctatatcctt tttgcttgcc
    26581 tgcatctacg cggccgacat aggcgatctt gagaggttcc gttttttctc ccggtttaaa
    26641 ttcacttaag tccacaccat taggaatgac gtgaactttc tgctgaaatt ggcgcatact
    26701 gttagccaca cgaactgaaa tgcaaataag cgctttagcc tctagcaaaa acggttgggc
    26761 ttcgcggctg ttgatgccta aaccatggca ggtgatgact aacgaggctt gaaaatgtct
    26821 tgccagggtc attgctaaag cataagttaa aggagaatga gcatgaatga cctgggggcg
    26881 ccactgagcg aggtagttta agctagcttg cagatcacca ggcttaacaa tggcggcacc
    26941 tatttcctcc atcgtttgtt tatagagagc taacgcttga taagaggggt tgccgtccat
    27001 aatcaaacgc gcatcaagtc cttgtttttg ttgctctcgc acgagagaaa aggcatgtgt
    27061 tgtttgccca gaataaaaaa accgattaag atgaaggatt ttcaaattat ttcacccctt
    27121 tcttttatta ttgtatgaag aggaaaagta aggggtgaac acaatataat tgctgctcat
    27181 agaaaaaagg agtatgaaac gagttcatac tcctcagagc ttaacgcacg ccaataaaca
    27241 tttgtgtcac atagacttga cgcccgagct gataaacgcc gatccccgtt tgattaaaac
    27301 gtgaattcag cagggcattg cgatgtatgc tgctgcccat aaaaagcttt acagcattgg
    27361 cagcagatga tgttttagct aaattctcac cagcataact ataacgaatg ccgttattac
    27421 gcatcatatc ataagcactg ccataagttg gtgaacggtg agcaaagtaa cggttctctg
    27481 ccatatcttt actttttaag cgtgctaatt tgactagctg ataattaatt gttagtggtt
    27541 ggagacctct tagtgtgcgt tcacgattaa taccagcgaa aacagttttt tctgccaggg
    27601 taaggctggt tggattaatt gcgggcgtgg gagcaggtgc tggggcaggt tccagtttag
    27661 gcgctggtgt tggttgtgca ttttggaatt gagcaaacca agcaggttgc ggccaacggg
    27721 gagggagagt tgttgcggat acgctgcctg cagtcattaa aactactagt aaagtcactg
    27781 taagaaccat cgagagacgt ttcatctaaa acctccgcat gcgcatattt tataacatct
    27841 atagcgtaac aaattgcttg tccttaggtc aaacagaaag aagtagggta ccctagcaat
    27901 atcttgtagt aagtacagcc tgatttgtta caatctctat tctggacaga agaggctatt
    27961 ttttaggaga tgtcttattc tggaacaccc ctgccaaagt accccaaaca ccttttttat
    28021 tttctaactc tgtttctagt agggctattt tcttactttg ttctttttct ttgtttagtg
    28081 ccgccacaag atcattgcgt aaacgtgcca gttgttttct ttgttcgcta tttaaggatt
    28141 ctgtgtgcga caaagtattt tttaattctg cattacttgt ttgcagtgcc tgcagttcct
    28201 gctccagccc atgattcttt tgctgcacct ctttactac
//
LOCUS       DASRMS010000199         1295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_53018, whole genome shotgun sequence.
ACCESSION   DASRMS010000199 DASRMS010000000
VERSION     DASRMS010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1295
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53018"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="VFC74_09460"
     CDS             complement(<1..746)
                     /locus_tag="VFC74_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25594.1"
                     /translation="MTECKFSADNLDFFLECFPIDKSSFTQLCGWIQAPKNLQQFIAV
                     AENLNELLSVSLHSELAAELGLDKLPTEQWRALLRDIQSQLPNIPRALADIQPNEQVR
                     PLKLIGSYPIYATLTASEVDYPTEEIEKIRVIHLIGMSAAIAYQQMHTAGVIKELSSG
                     FEAGLREIRDLEKTDKRPTVALPDWRGAVSLTEVQAELEEWIEKNSNDSKWGAGLRLL
                     IESVAEQRSRASRHYRMRMSTQIQRSGQHI"
     gene            complement(790..>1295)
                     /locus_tag="VFC74_09465"
     CDS             complement(790..>1295)
                     /locus_tag="VFC74_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25595.1"
                     /translation="MDSVHQPATGLLAEVYRVLSSVQFNAASQRQILEQIQQCSATDS
                     LGLPLIEVIQQRENSYLVCGNVRLYQLLQAAGAKILHAQILPAETCPNTRAGRAFRQG
                     INTILMYDCTAKSPHPQLRELKKHLKNHSSQELLELIKSDAFLKNALSISARDLRTKL
                     KKKALWN"
BASE COUNT          318 a          294 c          296 g          387 t
ORIGIN      
        1 ctaatgtgct gaccactgcg ctggatttga gtactcatgc gcatacggta atggcgtgat
       61 gctcgcgacc gttgttcagc gacagactct attaacagtc gtaggcctgc accccattta
      121 gaatcattgg aatttttttc aatccattcc tctaactctg cctgaacctc agtcagtgat
      181 actgcaccgc gccagtcagg tagtgcgact gttgggcgtt tatctgtttt ttctaggtcg
      241 cgtatttcac gcaaaccagc ctcaaaacca ctgctcaatt ctttaataac gccggcagta
      301 tgcatttgtt ggtaggcaat agcagcgctc atgccaatca ggtgaatcac tcttattttt
      361 tcgatttctt ctgttgggta atcaacttct gaagccgtca gcgtggcata aattggatag
      421 ctgccaatta gctttaatgg tctgacctgt tcgtttggct gaatatctgc cagcgctcta
      481 gggatgttag gtagctgcga ctgaatatct ctcagtaatg ctcgccattg ctctgtaggt
      541 aatttgtcta aacctagctc tgcagccagt tctgagtgta ggctaacgct caatagctca
      601 ttcagattct ctgccactgc aataaattgc tgtaagtttt tcggtgcttg aatccagccg
      661 cacagctggg taaaagacga tttatcgata ggaaaacact cgagaaaaaa atctaggttg
      721 tctgcagaaa atttacactc tgtcatgtta gctccagtaa tcaggtgtta gtttttaagt
      781 gtttgaccat caattccaaa gggctttttt tttgagctta gttcgcagat cccgtgcact
      841 gatacttagc gcatttttta aaaacgcatc gctcttaatt aactccagca attcttggct
      901 agaatgattt tttagatgtt ttttcagctc gcgcagttgt gggtgaggcg atttagccgt
      961 gcagtcatac atcaaaatag tgttgatacc ttgacgaaat gcacgtccag cacgagtatt
     1021 cgggcatgtc tcagccggca gaatctgagc gtgtaagatt tttgcacctg ctgcctgaag
     1081 cagctgatag aggcggacgt tgccgcagac taaatagcta ttctcacgct gctgaataac
     1141 ctcaataaga ggaagtccca gcgaatcagt agcgctgcat tgctgaatct gctcgagtat
     1201 ttgccgctga gacgcggcat taaattggac agatgataaa acccggtaga cctcggctag
     1261 tagccctgta gctggctggt gcactgaatc cattg
//
LOCUS       DASRMS010000200         3388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_120959, whole genome shotgun sequence.
ACCESSION   DASRMS010000200 DASRMS010000000
VERSION     DASRMS010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3388
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120959"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="VFC74_09470"
     CDS             <1..413
                     /locus_tag="VFC74_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007286595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HZK25596.1"
                     /translation="RIPAYIIIIATFVTMVDMLLNAYQPELHSQLGIFIPLIVVNCII
                     LGRAEAFASKNPVVSSVADGLGMGLGFTLALTILASIRELVASGTLFGANILGAAYQP
                     FKVFASPPGAFIALGLLMALVNSLAKNSAWHKEG"
     gene            417..992
                     /gene="rsxA"
                     /locus_tag="VFC74_09475"
     CDS             417..992
                     /gene="rsxA"
                     /locus_tag="VFC74_09475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013380941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HZK25597.1"
                     /translation="MTSILAIVFGAIFINNFVLSRFLGICPFLGVSKKVETSLGMGMA
                     VTFVMGMASLVTWLLEHYILEEFNLQYLRTIVYILVIAALVQFVELVIRKVSPVLYQA
                     LGIFLPLITTNCAVLGVALLNVQLEYNLLESVVFGIAAAIGFTLALVIMAGIRERLEL
                     VFIPKAMRGVPIALIVAGILSIAFMGFQGMM"
     gene            1005..1304
                     /locus_tag="VFC74_09480"
     CDS             1005..1304
                     /locus_tag="VFC74_09480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HZK25598.1"
                     /translation="MILPAVICLGGLGLIFGCLLAYAAQKFAVEVDPKIAEVQSVLPG
                     ANCGACGYAGCEQFAKAVAAGEAPVAGCKPGRAKVAAQIAEILGTEVPKAETGKK"
     gene            1736..3067
                     /gene="rsxC"
                     /locus_tag="VFC74_09485"
     CDS             1736..3067
                     /gene="rsxC"
                     /locus_tag="VFC74_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HZK25599.1"
                     /translation="MSPKTFKGGIHPGDFKSLTEKKPVVPAKPPAVAIIPLSQHIGAP
                     CEPLVAVGDEVKMGQKIGECEASISAPVHASVSGKVIKLGTCKSSQGRTINAVFIEND
                     FQDTWHESVKPNPDWEQIPAEEIPKIVREAGVVGMGGAAFPCHYKLVPPEQVEYVVVN
                     GAECEPYLTCDHRLMLERPDDLVVGVKLVMKAVNAKKGLIGIEDNKPDAIEAVRKAAA
                     DEPDVEVHALPTKYPQGSGKQLVSVLTGRETPSGCQSTTVGCLVQNVGTCVAVADAVR
                     FGRPSIERIVTITGAGVKEPKNLLVRVGTLLEEVVAECGGFNDNLRKLILGGPMMGPT
                     QSGPADLPVVKGTCGILCLTEQEVQMEEINPCIKCGKCVDVCPMGLMPLFLASAVEHQ
                     LYDKAEAYHAVDCIDCGCCSYICPAKRPINQWISMAKNEIKAKKRRAAAAK"
     gene            3095..>3388
                     /locus_tag="VFC74_09490"
     CDS             3095..>3388
                     /locus_tag="VFC74_09490"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZK25600.1"
                     /translation="MDKQLVVSSAPHLKSPETIKSVMVDVLVALVPAAIAAVTFFGTR
                     ALLTMVLTTLSAMLTEALILRNKNIFGDGSAAVTGLLLAMTLPPAPPWWLCVLG"
BASE COUNT          856 a          656 c          869 g         1007 t
ORIGIN      
        1 tacgtatccc ggcctatatc atcatcattg ccacctttgt gacgatggtg gatatgctgc
       61 ttaatgctta tcaaccagag cttcactccc agttaggcat ttttatcccc ttaattgtgg
      121 tgaactgtat tattttaggg cgggccgaag cttttgcctc caaaaacccg gtagttagct
      181 ctgtagcgga tggtctgggc atgggtctgg gctttaccct cgcgttaact attcttgctt
      241 ctattcggga actggtggcc agcggcacac tgtttggcgc caatatttta ggggcagctt
      301 atcaaccctt taaagtgttt gcttcaccgc caggtgcgtt tatcgccctg ggtttactaa
      361 tggcgttagt gaatagttta gcaaaaaatt cagcttggca taaggagggg tagataatga
      421 cgagtatctt agcaattgtc tttggtgcga tctttattaa caactttgtc ttgagcagat
      481 ttctcggtat ctgcccgttc ctgggtgttt ccaagaaagt agaaacgtcg cttggtatgg
      541 gtatggcagt tacctttgtt atgggtatgg cttctttggt aacttggctg ttggaacact
      601 atatcttaga agaatttaat ttacagtatc tgcgtaccat cgtgtacatt ttggtaattg
      661 cagcgctggt gcagtttgtt gaattagtaa tccgcaaagt aagtccggtc ttgtatcagg
      721 cactgggtat tttcctgccg ctgattacca ctaactgtgc tgttcttggc gtagcgctct
      781 taaatgtgca gctggagtat aacttattag aatctgtggt ctttggtatt gcggcagcga
      841 ttggctttac gctggcgcta gtgattatgg ccggtatccg tgagcgttta gagttggttt
      901 tcattcctaa agccatgcgc ggtgtaccaa ttgccttaat tgttgcagga attctatcca
      961 ttgcttttat gggctttcaa ggcatgatgt aggaggcgta gtaaatgatc ttaccagcag
     1021 ttatctgcct gggaggcttg ggccttattt tcggctgttt gctggcttat gctgcacaaa
     1081 agtttgctgt tgaagtggac ccaaaaatag cagaggtaca aagcgtttta ccaggtgcta
     1141 attgtggtgc ctgtggctat gccggttgtg aacagtttgc caaggctgtg gcagctggtg
     1201 aggctccggt agctggctgc aagcctggac gtgccaaagt ggcagcacaa attgctgaaa
     1261 ttctaggcac tgaagtgccc aaggcagaaa ccggtaagaa gtgattgccg cgctatcctt
     1321 gagtattgta cggtaaagat gtaaaatatt actgcaaaaa gaaaatgtaa cgaaaaacgg
     1381 tgctgaagtg aagtttcagc accgttttgg cgtgtaaaag atgcagaggc atctaaagac
     1441 ttttaaaggc atcctgcctc ctttgacaaa attcgcatat tcgttgtaca ctattgtcaa
     1501 cacctgtata gagaagctag tgcgaaatag gataggtata atgggaatct gcagattctc
     1561 attgtttttt gttgctggca acattttttt tactgtgttt tggctaatcc catttctgca
     1621 aaaggctgag aaaatattaa caagggcagg attattaatt attttctcga atattgctat
     1681 gttggccctt attgtaatct tttattaata atttaagtaa gaggtgaatt agtatatgag
     1741 ccctaaaaca tttaagggag gcattcatcc gggggatttt aagagtctta cggaaaagaa
     1801 gcctgttgtt ccggcaaagc cccccgcagt ggcgataatt ccattgtcgc agcatattgg
     1861 tgcaccgtgt gaaccattgg tagccgttgg tgatgaggtg aaaatggggc agaaaatcgg
     1921 tgagtgtgaa gctagtattt cagctcctgt tcatgccagt gtttctggta aagtaattaa
     1981 gttgggaact tgtaaatcta gtcaaggacg tacaattaat gccgtcttta ttgaaaatga
     2041 tttccaggat acttggcatg aaagcgtaaa acccaaccct gactgggaac aaatcccggc
     2101 cgaggagata cctaagattg ttcgtgaagc aggtgtagtg ggtatgggtg gagcagcatt
     2161 cccctgccat tataagctgg ttccgccgga acaggttgaa tatgtggtag taaacggcgc
     2221 tgaatgcgag ccttatttaa cttgtgacca tcgtttaatg ttagaacgtc cggatgattt
     2281 agttgttggt gttaagttgg tgatgaaagc agtaaacgct aaaaaaggct taatcggtat
     2341 tgaggataac aagcctgatg ctattgaggc cgtaagaaaa gctgctgctg atgaacctga
     2401 tgtggaggtt catgctctac caactaaata tccccagggt tcaggtaaac aattagttag
     2461 cgttttgact ggtcgggaaa ctccttcagg ctgccaatca acaacagttg gttgtctggt
     2521 acaaaacgtt ggtacctgcg ttgccgtagc cgatgctgta cgttttggca ggccttcgat
     2581 tgagagaatt gtcacaatca ccggcgcagg tgttaaagag ccgaaaaatc tattagtgcg
     2641 tgtaggtacg cttttggaag aagtagttgc agagtgtggc ggttttaatg acaatctgcg
     2701 caaattaatt cttggtggcc cgatgatggg tccgactcag tccggaccag cagatttacc
     2761 agtagttaag ggtacctgcg gtattttatg cctcacggag caagaagtgc agatggaaga
     2821 aattaatccg tgcattaagt gtggtaagtg cgtcgacgtt tgtcccatgg ggttaatgcc
     2881 actcttctta gcctctgctg tagaacatca gctgtacgat aaagcggaag cttatcatgc
     2941 ggtagattgt attgattgtg ggtgctgttc ctacatttgt cctgctaaac ggccgattaa
     3001 tcagtggatt agtatggcta agaacgaaat taaagccaaa aaaagacggg ccgctgcggc
     3061 taaataagaa gcaaaggtaa ggagggatac cctaatggat aaacaattag ttgtttcatc
     3121 agcgccacac ttaaaatcac cggaaactat caaaagtgta atggtggacg ttttagtggc
     3181 gttagtacca gctgcgattg ccgctgtaac cttctttggc actcgcgctc tgctcaccat
     3241 ggtactcacc accctgtctg ccatgttgac agaagcactt attctgcgca acaaaaatat
     3301 ttttggcgat ggcagtgcgg ctgtcaccgg tctgctgcta gccatgactt taccgccggc
     3361 ccccccttgg tggctatgtg ttttaggt
//
LOCUS       DASRMS010000201        11507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_793569, whole genome shotgun sequence.
ACCESSION   DASRMS010000201 DASRMS010000000
VERSION     DASRMS010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 11507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11507
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793569"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..2465
                     /locus_tag="VFC74_09495"
     CDS             333..2465
                     /locus_tag="VFC74_09495"
                     /inference="COORDINATES: protein motif:HMM:NF039835.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="InlB B-repeat-containing protein"
                     /protein_id="HZK25601.1"
                     /translation="MGLKYSVLRKPVNYLLVLLLVILQVVIAAPAEAAFSWQIVGSGS
                     VSTDIASYISLGVADNGTEYVAYSAVNDEYKATVRKHVAGEWLSVGPDKLDFKHASFI
                     NLRVHNNTPYLSFTSTPGSPQPSYAYVLKFDSSSNTWKTLGGGSVTNGVAWYNSLYIT
                     DDGTIYVAFLDKFEPQGGYGGILKVFVYNETHDTWTRIHHAEDSELRLNHSFLTLAVH
                     NQTPYVSYRDTSTEKAAVVKYDASTMRRTTLDDFASSTPVANSVSLSLGQDGTPYVAF
                     GKDGQYEKGATVMKYTTAEGWQRVGDSVSSYTSQQHKLVVYNNIPYLVFHDSNFKCVK
                     FTGDSWLPFAETPAGALDFVMHGGVPYVTYAAPADTSAIVLKGVEDTFTVTVQSADGT
                     VTGAGNYAPGAKVTLQATPKDSFDFVNWTDAENKVSTQNPYTFTMPAANVAITANYQQ
                     RTPVTYQVTVTSANGTVTGAGSYEAGAQVTLQATPKDGFDFVNWTDAENKVSTQNPYT
                     FTMPAADVTITANYQQQTTTPGTYKVTVTANNGAVTGAGSYKKGDPVTLQAAANTGYQ
                     FVNWTTSSGKVLTTQNPYIFNMPEGNVVLAANFAQLQATKHAVQVSATNGTVKGSGDY
                     AAGVTVRLEAVAASGYELVSWTTTDGVVVSRANPYVFVMGNEAISLQANFQETEVPEL
                     PKTSGHAQVYYEIGLLLAAVGIYLKRKR"
     gene            complement(2528..3817)
                     /gene="purD"
                     /locus_tag="VFC74_09500"
     CDS             complement(2528..3817)
                     /gene="purD"
                     /locus_tag="VFC74_09500"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HZK25602.1"
                     /translation="MRVLVVGGGGREHVLVWKLLQSPHVQKVYCAPGNAGIAEIAECI
                     PISTDNIAALVDFALEQEIELTVVGPELPLTAGIVDEFTAAGINIFGPNRAAAKLEGS
                     KVFAKQLMEKYQIPTAESRTFTNVDKALAYLQEKGVPIVVKADGLAAGKGVVVAATLK
                     EAEIAVQRMLVNEEFGEAGKRVIIEEYLTGEEVSVLAFTDGQTVIPMVSSQDHKAAYD
                     HDAGPNTGGMGAYSPAPVFTEQLLSQVEATILQPTIAGLQAEGIVFKGVLYAGLMITA
                     DGPKVLEYNVRFGDPECQVVLPRLESDLATVLLAVANSNLAGHKLKWYQNHTACVVMA
                     AGGYPGAYQKGKIITGLTEVRQHNDAYVFHAGTAKKGTKVVTNGGRVLGVTAWGDTLQ
                     AALDKAYQVVEKINFADAHFRRDIGHKAIKRLDKASK"
     gene            complement(3834..5375)
                     /gene="purH"
                     /locus_tag="VFC74_09505"
     CDS             complement(3834..5375)
                     /gene="purH"
                     /locus_tag="VFC74_09505"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HZK25603.1"
                     /translation="MTTKLALLSVSDKTGIVGFARGLAAQGYTIISTGGTQKTLADAG
                     VPVQSVAEITGFPEILDGRVKTLHPYIHGGILARRDLASHQQQLAEHQIKTIDLVAVN
                     LYPFAATIAKKDVTLAQAIENIDIGGPTMVRSAAKNYKYVTVAVNPARYADILTELQA
                     NGTVSEAMRYQLAVEAFSHTAEYDALISDWLYQQLEEAPRFAPTMVLPFSKVQDLRYG
                     ENPSQQAAFYREPQAAVGTVASAQQLHGKELSFNNLNDLNAAWEMVQEFDEPAAVAVK
                     HANPCGIAVAADLFTAYVCAYEADSVSIFGGIVALNRTVDAQTAKKMSEIFLEVIIAP
                     DYEDEALTILTSKKNVRILRAPLPAARAAIDVKKVSGGLLIQTLDQKVISPATWQTVT
                     KAEPTPQQIADMVFGMKVVKHVKSNAIVLVKNGQTVGIGAGQMNRVGAARIAIEQAGA
                     KADGSVLASDAFFPFADTVEAAVQAGVTAIIQPGGSQNDQDSIDRCNEQGLAMMLTGV
                     RYFKH"
     gene            complement(5365..5976)
                     /gene="purN"
                     /locus_tag="VFC74_09510"
     CDS             complement(5365..5976)
                     /gene="purN"
                     /locus_tag="VFC74_09510"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HZK25604.1"
                     /translation="MKKIAVLASGSGTNLQAIIDAVAAGTIKGAQVALMISDKQNAFA
                     LKRAQEHQIPTKYFNSKNFTTREDYDQAIITYLQEQQIDLVILAGYMRLVTPAFVAAF
                     PQRIMNIHPALLPAFAGTQGVADALNYGVKVTGCTVHFVDEGMDTGPIILQEAIPVND
                     HDTTESLHQRIQVLEHKLYPQAINLWLAGRLKIEGRRCLVDDN"
     gene            complement(6001..7050)
                     /gene="purM"
                     /locus_tag="VFC74_09515"
     CDS             complement(6001..7050)
                     /gene="purM"
                     /locus_tag="VFC74_09515"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020492831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HZK25605.1"
                     /translation="MIITGGAAVVSYTYKESGVDIDAGNEAVRLLRDHVRSTHRAEVL
                     TDIGGFGGLFALKQQYCEPVLVSGTDGVGTKLKIAFLLDKHDTIGQDAVAMCVNDIVV
                     QGAEPLFFLDYLAVGKLQPEKVAAIVSGIAAGCRLAGCALIGGETAEMPGFYANDEYD
                     LAGFAVGVAERSRLVDGSCTQVGDVVIGLPSTGLHSNGYSLARKVLLEKTGWAMSMYR
                     PELGKTLGEEMLTPTKIYVSTVLKLLAEVKVCSMAHITGGGLLENVPRSLPEGLGVTI
                     KKDAWQVPAVFKLIQEQGRVPEKEMFRTFNMGIGYVLIVPQQEAELAINLLQQAGEKP
                     VLLGAVVSGTGVQFS"
     gene            complement(7007..8473)
                     /gene="purF"
                     /locus_tag="VFC74_09520"
     CDS             complement(7007..8473)
                     /gene="purF"
                     /locus_tag="VFC74_09520"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HZK25606.1"
                     /translation="MGFEVTDLEADKLHEECGVIGIYAPDSDVAQLTYYGLYALQHRG
                     QESAGIAVANGKAINGEKGVGLVSEVFQDTSKFKRLQGQIAVGHVLYAADVSSLPANV
                     QPLMMRYRNGHLAIVHNGQLVNGQQLRLQLENEGSIFQTTSDSEVMAHLIARSGETDI
                     VAAVKKSLLSLQGAHTYLLMTPNKLIGARDPHGFRPLSLGKTSHGYVLASETCAFDSI
                     GAEFVRDIEPGELVVIDQDGVHAEYFTKPAKQALCIFEFIYFARPDSNIHGRNVHLVR
                     KRLGRRLAQEHPVEADIVTGVPDSSLSAASGVAEELGLPYEMGFIKNRYIGRTFIKPA
                     QEIRALGVRLKLNAVRQIVEGKRVVMVDDSIVRGTTSMHIVEMLRKAGAKEVHVLISS
                     PPVVAPCFYGIDTSSKGELIAARMTVEEMKEAIGADYLGFLSAEGMLECTNLAPDGFC
                     TACFNKQYPIEVNDCPLDKKLIKENDNNGRCSGCVLHV"
     gene            complement(8552..9079)
                     /gene="purE"
                     /locus_tag="VFC74_09525"
     CDS             complement(8552..9079)
                     /gene="purE"
                     /locus_tag="VFC74_09525"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004034864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HZK25607.1"
                     /translation="MKTPPLVGIVMGSDSDLPVMRAAAEIMEEFKVPYELAVMSAHRT
                     PARVEQFAQSAAARSLRVIIAGAGGAAHLAGVVAAFTTLPVIGVPILTKALAGADSLY
                     SMVQMPAGIPVATVAINGAKNAGLLAVQILATADEALSKQMVNYKEKLRQQVEVKADQ
                     LSALGWRKYLAAQEV"
     gene            complement(9208..10494)
                     /locus_tag="VFC74_09530"
     CDS             complement(9208..10494)
                     /locus_tag="VFC74_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003475389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HZK25608.1"
                     /translation="MEKFFKLKEHNTNARTEIVAGITTFMTMAYIIFVNPDMLSATGM
                     DKPALVTATILAAAITTILMGLVTNLPFALASGMGLNAFFAFTVAPQYGWEAALGAVL
                     ISGIVFFVFGVFGIIDQIDKAIPTSMKRAVAAGIGLFIALIGLINAGIIVGNPATVVA
                     LGNIKEAGPMVALIGLLITAVLMALKVKGAILIGILLSTIIGMFNGVTQIPQSIGDLV
                     GAPASIAPILFKLDFPGALKLGFMVIFSLVFVDMFDTLGTLMGTSARAGFLDKDGRLP
                     QIKGAMIVDAIGTMFGACLGTSTVTTYVESSAGISEGGKTGLTAVTTGVLFLLALFFT
                     PLALIVPGAATAPALIIVGVLMMGAVTEIDFNDFTEAFPAFLAIAIMPFSYSIASGIS
                     AGFIAYPLMKVAAGRAKEVHWFSYILFVISLAYFLL"
     regulatory      complement(10774..10875)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11239..>11507
                     /locus_tag="VFC74_09535"
     CDS             11239..>11507
                     /locus_tag="VFC74_09535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25609.1"
                     /translation="MENQFYDQQYLKLCSIADKEGRAFARTALESFADRESLQMFLNS
                     LFYNHSIMESDQFAVYLGLMYEFIGQEMPDDMDCYIVMDQESNIF"
BASE COUNT         3207 a         2903 c         2408 g         2989 t
ORIGIN      
        1 aatatactga atatttattc aatggaattc cccttgaaaa aacccctcag ctaggggaag
       61 caagcaattc aagattgaga taatttccgc tccacaaaaa tcttctgccc tcagtcttag
      121 ctcatagaca tcgccataga acgcctaagg cgatgagcag cgtaagataa aaaagggaac
      181 ttaaaaaata gaacagcgac cataaaatta ttttatagta cctaggcgaa ataagacaaa
      241 tttcgagaca ggaaaaggaa aaactttgca taatttaaca agcgaataac gtaaacgtca
      301 gaaatttttt tagaagagga aagggtggtc taatgggatt aaagtacagc gttttacgta
      361 aacctgtcaa ttacctttta gtattactgc tggtaatctt acaggtcgta attgcggcgc
      421 cggcggaagc agccttcagc tggcaaattg tcggcagcgg tagtgtttcc acagatatcg
      481 cctcctacat ttcgctgggg gttgcagaca atggaacgga atatgtagcc tattcagcag
      541 tcaatgatga atacaaagca actgtacgca agcatgtagc cggtgaatgg ctgtctgtag
      601 gaccagacaa gctcgatttc aagcatgctt ccttcatcaa tcttagggtt cataacaata
      661 ccccctatct gtcgttcacc tctacaccag gtagtcctca gccttcatat gcctatgtat
      721 tgaagtttga cagttcttcc aacacctgga aaactttggg cggcggctct gtcaccaacg
      781 gcgtagcgtg gtataattct ctttatatca cagacgatgg cactatctat gtggctttcc
      841 tagacaagtt tgaaccccag gggggatatg gcggcatctt aaaggtcttc gtctacaacg
      901 aaacccacga tacgtggacg agaatacatc atgcagagga tagtgaactc cggctcaatc
      961 acagtttttt aaccctagcc gttcacaacc aaacaccgta cgtctcatat cgtgatactt
     1021 caaccgaaaa agcagcggtt gttaagtacg acgcctcaac gatgaggagg acgacattag
     1081 atgacttcgc ttcctctacc cctgtcgcca actctgtctc tctcagcctg gggcaggatg
     1141 gtacacccta cgtagccttc ggtaaagatg gtcaatatga aaaaggtgct acggtgatga
     1201 aatatacaac cgctgagggg tggcagcgtg tcggtgacag cgtttccagt tatacatcac
     1261 agcagcataa gcttgtggtc tacaataaca ttccctatct ggtgtttcac gacagcaact
     1321 tcaagtgtgt aaaatttact ggcgacagtt ggctgccttt tgcagaaacc cctgccggtg
     1381 ccttggattt cgtgatgcac ggcggcgtac cttatgtcac ttacgctgcc cctgctgata
     1441 cttcggcaat tgtgttaaag ggtgtcgaag ataccttcac tgtaaccgta caatctgcag
     1501 atggtaccgt tacaggtgca ggaaactatg cgccaggcgc gaaggtaaca ctgcaggcga
     1561 cgccaaagga cagctttgat ttcgtcaact ggacggatgc cgaaaacaaa gtaagcacac
     1621 agaaccctta cacctttacc atgccagccg caaacgtggc gataactgca aactatcagc
     1681 aacgaacccc tgtcacctat caggttaccg taacttcggc taacggtact gtcacaggtg
     1741 cgggaagcta tgaggctggc gcgcaggtaa cactgcaggc gacgccaaaa gacggctttg
     1801 atttcgtcaa ctggacggat gccgaaaaca aagtaagcac acagaaccct tacaccttta
     1861 ccatgccagc cgcagacgtg acgataactg caaactatca gcagcaaaca acaacccccg
     1921 gcacctataa ggttaccgta acagcgaata atggcgctgt tacaggtgcg ggaagctata
     1981 aaaaaggtga tccagtgact ttacaagccg ccgctaacac tggctatcag ttcgtgaatt
     2041 ggactacctc atccggcaaa gtattgacaa ctcaaaaccc ctacatcttt aacatgcctg
     2101 agggaaatgt agtgttagct gcaaattttg ctcagcttca ggcaacaaaa catgcggtgc
     2161 aagtttccgc caccaacggg acagtcaaag gaagcgggga ctacgctgca ggggtaacgg
     2221 tgcggctaga ggcagtggct gcgtcgggat atgaacttgt cagctggacg accacagatg
     2281 gtgttgtggt ctcccgcgcc aatccatatg tctttgtgat gggcaatgag gcgatctccc
     2341 tgcaggcgaa cttccaggaa acagaagtac cggaactacc aaaaaccagc gggcacgctc
     2401 aggtctatta cgaaattggc ctactactgg cagcagtagg aatatatctg aaaagaaagc
     2461 gataatttat aaatcactaa atgataaacg gggcaaaacc tgattacttt ggttttgccc
     2521 cgttttttta tttacttgcc ttgtctagac gtttaatagc tttatgacca atatcccgac
     2581 ggaagtgagc gtccgcaaaa ttaatcttct caacaacttg gtatgctttg tcgagggcag
     2641 cctgtaaggt gtccccccaa gcagtgacac ccagtacacg accaccattg gttactacct
     2701 ttgtgccctt ctttgctgtg ccggcatgaa agacataggc atcgttatgc tgcctaacct
     2761 ctgtcaaacc ggtaataatc ttaccctttt gataagcacc aggataacca cctgccgcca
     2821 ttactacaca agctgtatgg ttctggtacc actttaactt atgaccagca aggttactgt
     2881 tggcaacagc caacagaaca gtggcgagat cactttctag acgaggtaaa acaacttgac
     2941 attccggatc gccaaaccgc acattgtatt ctagtacttt gggtccatca gctgtaatca
     3001 tcaagccggc ataaagtact cctttgaaaa caataccttc tgcttgcagt ccagcgatag
     3061 taggttgtaa aattgttgct tccacttggc tcagcagctg ttcagtgaaa accggcgctg
     3121 gtgagtaggc ccccatgcca cctgtatttg gtccggcatc atggtcatag gcggctttat
     3181 gatcttgaga ggaaaccatg ggaattactg tttgtccatc tgtgaaggcc aaaaccgaga
     3241 cctcttcgcc tgtgagatat tcctcgatta tcacacgttt gccagcttca ccaaattcct
     3301 catttaccag catgcgctgc acagcaattt ctgcttcctt taaagtcgca gcgaccacta
     3361 cacctttacc tgctgctaaa ccatcagcct taacaacaat gggcacgcct ttttcctgga
     3421 gataggctaa agctttgtct acattagtaa aggttctgct ttctgccgtt ggtatttgat
     3481 atttctccat cagctgcttg gcaaaaactt tactgccttc caacttggcc gctgcccggt
     3541 tcgggccaaa aatgttaatg cctgctgctg taaattcatc cacaatgcct gccgtgagag
     3601 gcagttctgg tccaacaact gtcaattcaa tttcctgctc cagcgcaaag tcaactaaag
     3661 ccgcaatatt atctgttgaa atgggaatgc attcggcaat ctcagcaata ccagcattac
     3721 caggtgcgca atatactttt tgcacgtggg ggctttgtag taacttccag acaagcacat
     3781 gttcacgccc gccaccgcct acaactaaaa cacgcattgt ttgcctcctt cgtttagtgc
     3841 ttgaaataac gcacgcctgt caacatcatc gccaatccct gctcattgca gcgatcaata
     3901 gaatcttgat cgttttgtga accgccgggt tggataatgg ctgtgacgcc ggcctgcact
     3961 gctgcctcca ctgtgtcagc gaaggggaag aacgcatctg acgctagcac actgccatct
     4021 gctttagcgc cggcctgctc aatggcaatc cgggcagcac caacacgatt catttgtccg
     4081 gcgccgatgc ctactgtctg accattttta actaacacaa tagcgtttga tttaacatgt
     4141 tttactacct tcataccaaa aaccatatct gctatttgtt gtggcgtagg ctctgctttg
     4201 gtaacagttt gccaagtcgc aggcgaaatt actttttgat ctagtgtttg tatcagtagg
     4261 cctcccgaga cctttttcac atctatggcc gcccttgctg caggcagtgg tgcgcgcagg
     4321 atacgaacat tctttttgct ggttaaaata gttaaggcct cgtcttcata gtccggggca
     4381 attattacct ctaaaaatat ttcactcatc ttcttcgctg tctgggcgtc tacagtgcgg
     4441 tttaaggcga caataccacc gaaaatggag acagaatctg cctcataagc acatacataa
     4501 gcagtaaaca gatctgcagc cacagcaata ccacaaggat tagcatgctt aaccgctaca
     4561 gcagccggtt cgtcaaactc ctgcaccatc tcccaagccg catttaaatc attaagatta
     4621 ttaaaggata attccttgcc atggagctgt tgggcactgg ctacggtacc gacagcagct
     4681 tgtggctcac ggtaaaaggc agcttgctgg cttgggtttt caccgtagcg aagatcttgc
     4741 accttactaa aaggcagtac catcgtaggc gcaaaacgcg gtgcctcttc taactgttga
     4801 taaagccagt ccgaaattag ggcatcgtat tcggcagtat gactaaaagc ctccactgcc
     4861 agttggtagc gcatagcctc actgacagtg ccgttggcct gtagttcagt taaaatatct
     4921 gcatagcggg caggattaac agccacagta acatatttgt agttcttagc cgcagaacgc
     4981 accatggtgg gaccgccaat atcaatgttt tcaattgcct gtgccaaagt aacatccttt
     5041 ttagcaatgg tagccgcaaa aggatataaa ttgactgcaa ctaaatctat cgtcttaatt
     5101 tggtgctctg ccaattgctg ctgatggctt gctaaatcac gccgcgccaa aattccgcca
     5161 tggatgtagg gatgcagcgt ttttaccctg ccatcaagaa tttccgggaa tcctgtaatc
     5221 tctgctacac tctgcacagg cacacctgcg tctgctaatg ttttttgcgt gccaccggtg
     5281 gaaataatag tgtagccttg cgctgccaac cctcgagcaa agcccacaat acctgttttg
     5341 tctgagacac taagtaaagc cagtttagtt gtcatcaacc agacaccttc ttccttcaat
     5401 ctttaatctg cctgccagcc acaaattaat ggcttgcgga taaagtttat gttccaggac
     5461 ttgaatccgc tgatgtaaac tttctgttgt gtcatgatcg ttaaccggaa tggcttcctg
     5521 caaaataatt ggtcccgtat ccatcccttc atcaacaaaa tggacagtac aacctgttac
     5581 cttaaccccg taattcaggg catcggctac accctgcgta cccgcgaaag caggcagtaa
     5641 agctggatga atgttcataa tccgctgcgg aaaagcggcc acgaaagctg gcgtcactaa
     5701 acgcatatat ccggccagga taacaagatc tatctgctgc tcttgtaagt atgtaatgat
     5761 cgcctggtcg tagtcctccc gggtggtaaa attcttgctg ttaaagtact tggtgggaat
     5821 ctgatgctct tgcgcacgtt tcagggcgaa ggcattttgt ttatcactaa tcatcagcgc
     5881 cacctgcgcc cctttgatgg tacctgcggc cacggcatcg ataatcgcct gcaagttagt
     5941 gccgcttcca gaggccagca ctgcaatttt cttcatttct cgtcttccct tcccgacttt
     6001 ctagctgaac tgtacccccg taccgctgac aactgcgcct aaaagaactg gtttctctcc
     6061 ggcttgctgc agtaggttga tggccagttc tgcctcttgc tgtggcacta tcagcacata
     6121 acctatgccc atattaaaag tacgaaacat ctctttttcc ggtacccgac cctgctcttg
     6181 gattagttta aacactgcag ggacctgcca agcgtctttc ttaatggtca cccctaaccc
     6241 ctctggcaaa gagcgcggca cattctccag caaaccacca cctgtgatat gagccatgct
     6301 acaaactttc acttctgcca agagcttaag aacggtgctt acataaattt tagttggggt
     6361 cagcatctcc tcgccaagcg ttttgcctag ctccggccgg tacatcgaca ttgcccaacc
     6421 tgttttttct agcagtactt tgcgagctaa agaatagccg ttgctgtgca aacctgtgga
     6481 aggcagacca atcaccacat cgcccacctg tgtacaactg ccatcaacta aacgtgaacg
     6541 ctctgctacc cctactgcaa aaccagctaa atcatattca tcattagcat aaaacccagg
     6601 catttcagct gtttctccac caattaaagc acagccagct aaacgacagc cggcagcaat
     6661 gccgctgaca atcgctgcta ctttttctgg ctgcagcttg cccacagcca aataatccaa
     6721 aaagaataat ggttccgcac cctgcaccac aatatcatta acacacatcg ccactgcatc
     6781 ctgcccaata gtatcatgtt tgtctaacag aaaggcaatt ttaagtttgg tgcctacgcc
     6841 gtctgtccca gaaaccagca ctggttcaca gtactgctgt ttaagagcaa aaagaccgcc
     6901 aaacccgcca atatctgtta atacttcagc acggtgagtg gaacgaacat gatcacgcag
     6961 caaacgaact gcttcattac cggcatcaat atccactcca gattccttat acgtgtagga
     7021 cacaaccgct gcacctcccg ttattatcat tttccttaat taatttcttg tcaagagggc
     7081 aatcgttcac ttcaataggg tattgcttat taaaacaagc cgtacaaaaa ccatcgggcg
     7141 ctaagttagt acattccagc ataccttcag cactaagaaa gcctaaataa tcagccccaa
     7201 tggcttcctt catttcctcc accgtcatgc gggcagcaat taactcaccc ttggaagaag
     7261 tatcaatacc ataaaagcag ggagcaacta ctggtggaga actaataagt acgtgcactt
     7321 ctttagcccc agctttacgc agcatctcaa caatgtgcat cgaggtagta ccgcggacaa
     7381 tagaatcatc caccatcacc acacgtttcc cttcgacaat ctgccgcacg gcattaagtt
     7441 taagacgtac acctaaagca cgaatttcct gcgcaggttt tatgaaagtg cggccgatat
     7501 aacgattttt tataaaaccc atttcgtaag gcaagcctaa ctcttctgct acccctgaag
     7561 ctgccgacag tgaggaatcg ggtactccgg tcacgatatc tgcctctaca ggatgttctt
     7621 gtgctaagcg gcgtcctaaa cgtttcctga ctaaatgcac attacgtcca tgaatgttgc
     7681 tatccggtcg ggcaaaataa ataaattcaa aaatgcagag tgcttgtttc gccggcttgg
     7741 taaaatattc ggcatgtaca ccgtcttgat ctatgaccac cagctcaccc ggctcaatat
     7801 cgcgtacaaa ttcagcacca atggaatcaa aagcacatgt ttctgaagcc aagacgtaac
     7861 catgcgatgt tttgccaagt gacaggggac gaaaaccatg cgggtcacga gcgccaatta
     7921 atttgtttgg cgtcatcagc aaataagtat gcgcaccctg taaagaaagc agcgatttct
     7981 tgacagcagc tacgatatct gtttctcctg aacgggctat caagtgagcc attacttcac
     8041 tgtcactagt agtttgaaaa attgatcctt cattttctag ctgtaaccgc agctgctgtc
     8101 cattgaccag ctgtccgtta tgcactatgg ctagatgacc attgcggtaa cgcatcatca
     8161 acggttggac attagccggc aagctagaaa catcagcggc ataaagtaca tggcctacag
     8221 cgatctgccc ctgcagacgc ttgaatttac tagtgtcctg aaaaacttca gagaccaatc
     8281 ctacgccctt ttctccatta attgctttac cgttagctac ggcaatacca gcactttcct
     8341 gaccacgatg ctgcagtgca tagagtccat aataggtaag ttgagccacg tcactgtctg
     8401 gcgcataaat tcctatcaca ccacattctt catgcagctt atctgcttca agatctgtta
     8461 cttcaaatcc catgaacgag cctcctgcta aaacgttgtt agttagttga gaaacttgcc
     8521 ttatcaaaca gctgatgctt cttgtttgca gttacacttc ttgagcggct aaatactttc
     8581 tccaacctag tgcagaaagc tgatcagctt tgacttccac ctgctgacgc aatttttctt
     8641 tatagtttac catctgcttg cttaatgctt cgtctgccgt cgccaaaatt tgcacggcca
     8701 acaaaccagc gtttttagca ccattaatgg cgaccgtagc tacagggatg cctgcaggca
     8761 tctggaccat tgagtataaa gagtctgccc cagctaaagc cttggttaaa atcggcacac
     8821 caatcactgg tagtgtggta aaagccgcaa ccacaccagc taaatgggca gcaccaccgg
     8881 caccggcaat gatcacacgc aaactgcgtg cggctgctga ctgggcaaac tgttccaccc
     8941 gtgccggagt tcgatgggca gacatcactg ccagttcata gggcaccttg aattcttcca
     9001 ttatttctgc agccgcacgc atgacaggca aatcagaatc actgcccata acgattccca
     9061 ccaggggtgg tgttttcatt aatcttgtac ctcccgttaa tttaagtctg aagttagttt
     9121 gtctcaattt gtcgctgaaa agactgtctt tagcaaaaaa cgtgtaaaga taaaggggcc
     9181 gtagttatac gacccctgcc tgttgtctta gagtaaaaag tatgcaagtg aaattacaaa
     9241 gagaatatag ctaaaccaat gtacttcttt agcccgacca gcagctactt tcatcagcgg
     9301 gtaggcaatg aaacccgcgg aaatcccact agcgatggag taggaaaatg gcatgatagc
     9361 gatggctaaa aaggcaggaa atgcttccgt aaagtcatta aaatcaattt cagttaccgc
     9421 acccatcatt aagaccccga caattatcaa tgctggtgct gttgctgcac caggcacaat
     9481 taaagccagc ggcgtaaaga aaagtgctaa gagaaaaagc acaccagtgg tcacagcagt
     9541 aaggccagtc ttgccgcctt cggaaatacc agcggaggat tctacatatg tggtaacagt
     9601 acttgtacct aaacaggcgc caaacatcgt gccaatcgca tctacaatca tggccccttt
     9661 gatctgcggt aaacgtccat ccttatccag gaaaccggca cgagcgctag tacccattaa
     9721 agtgcccagg gtatcaaaca tatcaacaaa gaccagggag aatatcacca taaagcccag
     9781 tttgagggca cctgggaagt ccaatttgaa taaaataggt gctatactag cgggagctcc
     9841 aactaaatca cctatcgatt gaggaatttg ggtcacacca ttaaacatgc caataatcgt
     9901 agaaagcaga atcccgatca aaatagcgcc ttttactttt aaggccatta atacagcagt
     9961 aatgagcaaa ccaattaaag ctaccatagg acctgcttct ttaatattac ctaaggcaac
    10021 gacagtggcg ggatttccca caataatgcc agcgttgatt aaaccaataa gtgcgataaa
    10081 gagaccaatg cctgcggcta cggctcgttt catactagtg ggaatagcct tgtcaatctg
    10141 gtcaattatc ccgaaaacac caaagacaaa gaatacgatg ccggaaatta gcacagctcc
    10201 tagggcagct tcccaaccat attgcggagc tacagtaaag gcaaaaaaag catttaaacc
    10261 cataccggaa gccaaagcga aaggcagatt agtaacaaga cccattaaaa ttgtcgtaat
    10321 agcggcggct aagattgtag ctgtaactaa tgccggctta tccataccag tagcactaag
    10381 catatcaggg ttaacaaata taatataagc catggtcata aaggtagtaa ttccagcaac
    10441 aatttctgtc cgtgcattgg tgttatgttc tttcagttta aagaatttct ccatttggat
    10501 aacctccttt tattcctctc aataaagtgg ttagatactt agaaaagcga ctgcctgtat
    10561 ctctcacccc caaatactaa aagtcattta cgcacaatag acagcagcgg cagaataaat
    10621 tcagcagggg aactatccca aatatcctga accaatagaa aagcccagac aggtaggtcc
    10681 cttggagtga aacctacccg actgggcttt tatcccttcg gtgtagcaca ttgtgcctgc
    10741 gccacttgga cctgctcgca attgcgacgg aaccctaggc gcactttccc ccgtagtcag
    10801 ataatttacg gtcatctggt agaaactttc aggccatatt cctgaactta tacgaagata
    10861 ttaaattcac ctttagatta tcagattcga catagcctgt caataaccat ttatactatt
    10921 cccactctct gttttgactt tttcacgcgc tccagcgggc tgtttcacgc ggttctaagg
    10981 cattattttc agctttatcg acgctttttc tcgtctatta aggtcgatag tacgctttta
    11041 gtacggtttt ttcacgcatc tcaggcgtta attttgtctt cactattcta agttttgaac
    11101 tattatattc cgcagagact tctaaatcaa ttttattatg ttaatatctc acctctctgc
    11161 caccgcctct tttggggcgg ttttttattg aaggaaattt tcaaaataag catttaaaaa
    11221 gaatccagga gggtcacaat ggaaaatcaa ttttacgatc aacagtatct taaactctgc
    11281 tctattgcag ataaagaagg aagagccttc gctcgtactg cattagaatc ctttgccgat
    11341 cgagaaagtc tacaaatgtt tttaaactcg ctgttttaca accactccat tatggagagt
    11401 gatcagttcg ccgtttatct tggcctgatg tatgaattca tcgggcagga aatgccggat
    11461 gatatggact gctatattgt catggatcaa gagtccaata tcttttt
//
LOCUS       DASRMS010000202         1108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_258693, whole genome shotgun sequence.
ACCESSION   DASRMS010000202 DASRMS010000000
VERSION     DASRMS010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258693"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1108)
                     /locus_tag="VFC74_09540"
     CDS             complement(47..1108)
                     /locus_tag="VFC74_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HZK25610.1"
                     /translation="MQYIQEAFDTNWIAPLGANVNGFERELAAKVGAKHAAALTTGTA
                     AIHLALRALNIGEGDLVICPSLTFSATANPVIYQNATPVFVDSDYETWNMDPQALELA
                     FEKYGAKIKAVLVVHLYGLSTDMDRIMEICNSYNVPVIEDAAESLGGYYKGKHTGTIG
                     ELGVFSFNGNKIITTSGGGMLVSADEEKIKKVRFWSTQSRDPARHYEHSELGFNYRMS
                     NIVAGIGRGQLKVLDQRLAKKKYIFEFYKRELGSLMGVDFMPINHWNEPNYWLSVITL
                     EGKVRPIDLMKALEAQNIETRPVWKPMHLQPFFSDCDYIGGNVSEKLFENGVCLPSDT
                     KMTDKDLDRVCEAIKPLWE"
BASE COUNT          293 a          278 c          200 g          337 t
ORIGIN      
        1 agatttcagc ctcttcttat ttttgtttac agccattcta atcactctat tcccacaagg
       61 gctttatcgc ctcacacacc ctgtccaaat ccttatccgt catcttggta tcactgggca
      121 gacaaactcc attctcaaac aacttttcac tcacattccc accaatatag tcacagtcgg
      181 aaaaaaacgg ttgcaggtgc atgggcttcc aaactggtct ggtttctata ttctgagctt
      241 ctaatgcttt cattagatct attggtctta ctttgccttc cagggtgatc acgctcagcc
      301 aatagtttgg ctcattccag tgattgatgg gcataaagtc tacacccatt aaagagccaa
      361 gctctctttt ataaaattca aagatatatt tctttttggc caatctctga tccaatactt
      421 taagttggcc tctgccaatc ccggcgacaa tattgctcat gcgatagtta aagcctaatt
      481 cgctatgttc atagtgcctg gccggatctc tactctgcgt agaccagaat ctaaccttct
      541 taattttttc ttcatcggcg gaaaccagca taccaccgcc ggatgtagtg ataattttat
      601 taccgttaaa tgaaaaaact cccaattccc cgattgtacc tgtgtgtttg cccttataat
      661 agccgcctaa agactctgca gcatcttcta tgacaggaac attgtagcta ttgcaaattt
      721 ccatgatcct atccatatca gttgataaac catataaatg cacaactaaa accgctttta
      781 tcttggcgcc atatttttca aaggccagct ccagtgcttg gggatccatg ttccaggtct
      841 cataatcact gtcaacaaag acgggagttg cattctggta gataaccgga ttagctgtag
      901 cagaaaaggt aagggacggg caaattacaa gatcgccttc cccgatgttt aaagctctaa
      961 gggccaggtg aatagccgct gtaccggtag ttaaggctgc cgcatgctta gctcctacct
     1021 tggctgccag ttcacgctca aagccattta cattggctcc caaaggcgct atccaatttg
     1081 tatcaaacgc ttcttgtatg tattgcat
//
LOCUS       DASRMS010000203         1363 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_871326, whole genome shotgun sequence.
ACCESSION   DASRMS010000203 DASRMS010000000
VERSION     DASRMS010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871326"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..324)
                     /locus_tag="VFC74_09545"
     CDS             complement(148..324)
                     /locus_tag="VFC74_09545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25611.1"
                     /translation="MTKIIDELKERIVDELEELNDKEKWCPVCVEAMYYMIKTWKYLC
                     EIEEKEKLPPPSTK"
     gene            complement(755..>1363)
                     /locus_tag="VFC74_09550"
     CDS             complement(755..>1363)
                     /locus_tag="VFC74_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25612.1"
                     /translation="LANTFNTDIGNIQQSLCALNGGIDKLSGQIGLSGQQVINAIQTG
                     NMNIVQQLMNCCCETKALILEQANATRMATMEQTNTLSDRMRTGFAETHQLIDAQTAQ
                     MVAGFQSIKDMFTQNKIDTLQATVNQLQNNANNSAQTAYLQNYINSQLAPMQTGMNTL
                     MNRVPSNPVPAVVVGNASTGGYAYGGWNNSCCGCMYGNGNNG"
BASE COUNT          355 a          310 c          281 g          417 t
ORIGIN      
        1 ctagagcggc ttggctatat ggctcatctc tgcacggatg gcttcaatta tgattatgaa
       61 gaagaaacgg tagcaatgcc acttttcatt tcttgggccg cagaataaaa aaaatagggg
      121 actgccgcaa aggcaatccc cttaatttta tttagtagaa ggtggaggta gcttttcttt
      181 ttcttcaatt tcacacagat atttccaggt ttttatcata tagtacatag cttctacaca
      241 aactggacac catttttctt tatcattaag ctcttctaac tcatctacta tacgttcttt
      301 tagttcatca attatcttcg tcattgcggc caccccccct atttcactct ataaattaca
      361 atatttgcgg tgcttactac tcccgcagtt gcaattactt gcagagtaac tgtattattt
      421 acgcaattac aagaaggtag cacgttgaca attgtggtaa atcctaatga acccgcatta
      481 ttctcggcta cactagtgat tgttgtaaga gtttcgggct gcgctactcc atctttataa
      541 agctgaagac tgaaagctgt agtagcggtt gtcgcggctc ctacaccgtt gaaagcaatt
      601 acataccgtc ccggcgcact tatgcgaaaa ctggtcccgt tcgtgacacg caccctgcaa
      661 tcatcatact ttattgtcgg gaacgctatg ggagcattgg cggtataagt agtgctagtg
      721 ttactaaaag cttggaacat aagggacacc ccctttaacc attattacca ttaccataca
      781 tacagccgca gcagctattg ttccagccgc cataagcata gccgccagtg cttgcgttac
      841 caacgaccac ggctggaaca ggattgctag gcacgcggtt cattagagta ttcatacctg
      901 tctgcatagg cgctagctgg ctattgatgt aattctgtag atacgcagtc tgcgcgctat
      961 tattggcgtt gttctgtagc tgattaaccg tagcctgaag agtatcaatt ttattctggg
     1021 tgaacatatc tttaatgctc tggaagccag caaccatctg cgcagtttgt gcatcaataa
     1081 gctgatgtgt ttctgcaaaa cccgtgcgca ttctatcgct taaagtgtta gtctgctcca
     1141 tagtagccat tctagtagcg ttcgcttgct ccaaaattag agctttagtt tcgcagcaac
     1201 agttcattag ttgctgtaca atattcatat taccagtctg aatggcgtta atgacttgct
     1261 ggccacttaa accaatttgg ccgctcagct tatcgatgcc gccattaagg gcacacagag
     1321 attgctggat attgccaatg tcggtgttaa aagtgtttgc aag
//
LOCUS       DASRMS010000204        75922 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_871363, whole genome shotgun sequence.
ACCESSION   DASRMS010000204 DASRMS010000000
VERSION     DASRMS010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 75922)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75922)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75922
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871363"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..295
                     /locus_tag="VFC74_09555"
     CDS             182..295
                     /locus_tag="VFC74_09555"
                     /inference="COORDINATES: protein motif:HMM:NF020553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HZK25613.1"
                     /translation="MSLQARQITEDMTIAQVLRLKPNSVKVFMEVGMHCFG"
     gene            831..2222
                     /locus_tag="VFC74_09560"
     CDS             831..2222
                     /locus_tag="VFC74_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK25614.1"
                     /translation="MKKPLSPAIKTFYGFGDMGFSLMASVETFFFVYFLTNVAKFSLP
                     MVAIVGTITSVADAALSPFYGGIIDGTKPMKWGRVRSWMLLCPPIVVPLYIFQYSNIG
                     ANETVAAAIVCAGSILSHIFWNLPWVANLSLISVLANNPGERAFLSSRRATWTMIAGM
                     LFSFIGSTMAIKIGEVTGNEALGYQALAGLMGLVFLIGYWTVFKITDGYEETGAAAQA
                     SAAKGNQVSVGVMLKSALQNPPLLVLLVGDFFRYMVNFIMSSSAAYYFTYVAKNMALL
                     PIYLFVGSLGGVIGAYFAGILCNKYSTRTVSIWGLFAMALSLIACKFVAFNIILFFGL
                     VLVARTMLGVLSASIVALYSDAAVYSEWKTGKSAASFVMGLMTISLKAAVISRGTVIP
                     FVLATAGFVASADPATASLELQNAVVNVFVLIPGVLALISAFIIGLGYRLTRERLTEL
                     QAEIDARKAATAQ"
     gene            2362..2712
                     /locus_tag="VFC74_09565"
     CDS             2362..2712
                     /locus_tag="VFC74_09565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25615.1"
                     /translation="MKNLEEIKQKEKSVRETLLFKTTVLLDQLLTYVPTRSILAVNDI
                     PVKVICGYDGQKSLLINKRPLSTADDCEWVIENYEALKQAVEELVNPEAEEIQKVIER
                     AEELIEKVDNITKT"
     gene            2908..5463
                     /locus_tag="VFC74_09570"
     CDS             2908..5463
                     /locus_tag="VFC74_09570"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HZK25616.1"
                     /translation="MELEQTISMAIPLFAFFLYTGLMILSIKSSQTKVTRLFQNYLLL
                     MMDWSLGSFMMRTGYAPAPLFWNRMMVVGMTGVPFIFYRFSEALLSRAQSLTSKIGYF
                     LYGLLLIANFKGLLVQDVILSAGDLSYTIGPLAVPFALISGGYVLLATILLVGEGFKN
                     PASFWSNRLLYPSIGTVLMFIGCTLNLFPEIGKYPFDILTNTINAFLLTYAIYRYQLL
                     NITISIRKGLVYSFLTAMLTGSYLLLVYLLETVIRNNLGYTAFIIALIMAAIIAIIFE
                     PVKNWLRVRIDKVMFGEIYDYRKTLKQFSYLKASILDLDELTHSTLGLLTKALQVNSL
                     ALLLLDQESNFFVYASVNMDETVAKTVRIDKTSPIIRRLAQHNPILTWQEIETAPEFQ
                     GLWQLEKDFFIAMQISHFVGIKLGGELIGILVLPHKFSRDPYTDDDREMLLMLANEAA
                     VAIQNAQIYSEVKRQAVRDELTKLYNYRFFQEFMDKEIARCQRTKQVFSVIYLDLDFF
                     KAYNDIFGHLAGDEALVTVGRAISESIRPSDIATRMGGDEFGIILPGATSHNALGVAK
                     RIKNAVQAYFPGTSKDSVLTISQGIATYPQHSQNKQQLLSCADTALYEAKNMGRNRVC
                     LYQPQLVRSDGTTASSTTAEIERNFLIQQVEDAYLSVIYTLAAAINAKDNYTYQHSEA
                     VTYYSIAMAVILGFAEEQKKILRYAAMLHDIGKIGIPGYILNKPTALTREEREVIESH
                     VLVAEGIVSHTPYLREVAPIILHHHESYDGTGYPHGLIGEEIPIEARILTIADAYHAM
                     ISDRPYRQGMLPDVALQQMDKLSGRQFDPKLLPIFASIIREQQKRRAAGEAYA"
     gene            5447..6022
                     /locus_tag="VFC74_09575"
     CDS             5447..6022
                     /locus_tag="VFC74_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25617.1"
                     /translation="MKLMPSAARNIIAMGVAGYLLLLIYDLASLRKLPYRFLFSLLGY
                     SMQAYAIVSAALFEQQLAVSKYLWLLAWPLMLAGLSWLLYCLFLYPPLRRTYRDKKGP
                     ALTTEGPYALSRHPGVYGYTFFVLSLAVISRSQLLLSAGCLWSVINLIYVFLQDRFIF
                     MALLEGYEQYRRNTPMLIPNLQSIKRFWATK"
     gene            6041..7618
                     /locus_tag="VFC74_09580"
     CDS             6041..7618
                     /locus_tag="VFC74_09580"
                     /inference="COORDINATES: protein motif:HMM:NF015291.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HZK25618.1"
                     /translation="MITLQELLNKDRRKDAWLRHCGFIDLSLVEFQEVQERLLLEQIE
                     MLARSYLGKKLLHGNFPRNGEEFRKSIPLTTYQDYHPYFKDKQEDILPLKPSYWVCTS
                     GAIDAYEYKWAPFPPGLAQTHIKNFLASVIFSVCTKKGEFSLNEYAKFLYAMAPPPYL
                     TGMVPYGLEKEFPFTYLPSLETAEKMSFEERNNDGFRLGLTQGIDLFFGVSSVLVRMG
                     EKFAQTKNDFSHQEKLNIRALLRMARGMVRCKLQKRELLPRDIWSLKGIICAGTDTNF
                     YKERIEYYWGKKPLEIYGGTEIGIAGTQTWDYEGMTLFPDANYWEFIPEDEYFRSKAD
                     LNYQPTTVLLSELKAGKRYELVITSLKGGAFVRYRVGDMIRVLSLKNSVLSIDLPQII
                     YEDRIDDVIDLAGFTRIYERTIWEALKEAGITHSHWLACKAFTGGHPLVKLYLELPAG
                     CTQELMEDKIHQALCKVNADYDDLGHMMAYRPLQLAAAPSAAFAKLKEKQGQASVDPA
                     IFRRFNPPEYLIKELFA"
     gene            7926..9296
                     /locus_tag="VFC74_09585"
     CDS             7926..9296
                     /locus_tag="VFC74_09585"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25619.1"
                     /translation="MTDIRALEKEREQIFINLYRGKVPTRVPVSNPMALEACLEYAGA
                     NPVEVLWDVSKTEAVFDQVNTDFYSDTSVGGTRRYPSFYQVLGARSFVMSSTGAMQHP
                     NVHGMEAAEYDDFIDNPYDTIMEKILPRLYTELATDAATRAAVLAKAMNIRSSEFAVL
                     GRIGVKMREKYGFAAIPGNATTAPFDFIADFFRSFTGICSDIRRYPEKVIAACEAMTP
                     ITIKKGRIPFPSKIGVTGIPLHMAPFMRTKDFEKFYWPSLKKVVETFHAMGINASLFV
                     EHDFMRFLDYLSELPENTILRFEYGDPKIVKEKLGSKQIISGFYPVMLLHTGTKQQCL
                     DKAKELMDILAPGGRFWFNTDKGPMDMHGNIAENLRAVLEFVYDYGKYEHPDEPYNLA
                     HEPQPQADKVINEINANLNSKYYYTWQDYQTRYPELAQRTEQIIARQLQKNEDAFFDF
                     IINLCS"
     gene            9427..10674
                     /locus_tag="VFC74_09590"
     CDS             9427..10674
                     /locus_tag="VFC74_09590"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015759363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HZK25620.1"
                     /translation="MAENLNPFEIVQQQISEAGKYLNLDPAIEAVLKQPKRVLTVTFP
                     VKMDDGTTRVFTGIRSQHNDAIGPCKGGIRFHPDVTVDEVKALSMWMTFKCGVVGLPY
                     GGSKGGVICNPKELSQGEIERISRGYIEGIAAIIGPDKDIPAPDVYTNAQIMAWMMDE
                     FSRLRGTNQFGVVTGKPIIVGGSLGRDKATAQGCVFVVIKAVEKLGIDLNGATVAIQG
                     YGNAGSQAAFLLHKLGCKIVAVSDSRGGIYKPEGLDLAALYQHKTDTRSCINFPGSQC
                     VSNAELLELPVDILIPAALENQITASNAPRVKARIVAEAANGPTTPEGSKILYEHGTT
                     VIPDILANAGGVTVSYFEWVQNLMNYYWSEEEVFEKLQVNMDNAFDQVYAISQQHQVD
                     LRTAAFIVSIKRVAEAIKIRGIV"
     gene            10826..11110
                     /locus_tag="VFC74_09595"
     CDS             10826..11110
                     /locus_tag="VFC74_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017751729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HZK25621.1"
                     /translation="MTAYVYIVKCADNTLYTGWTTSLVRRVRVHNQGKAAKYTRARRP
                     VELVYWERLDSKVAALQREWEIKQLTRAEKLALCNMKLKYLLRERGDETT"
     gene            11187..11675
                     /locus_tag="VFC74_09600"
     CDS             11187..11675
                     /locus_tag="VFC74_09600"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HZK25622.1"
                     /translation="MKQNKKLVWVLVFVLTALEQGIKIIINRYYLQVKVPLVPPWIYF
                     APLFNRDYSWINSLLQLGVGKVVHIMIVVAAIILIYFFYRYVADRYKVTALVGISFAL
                     FFAGALCSLLDKVFWDGSLDYLLVTGFFTFDLKDAYLNVAVALIVLMLAIDHQGMRTA
                     EF"
     gene            11881..13437
                     /locus_tag="VFC74_09605"
     CDS             11881..13437
                     /locus_tag="VFC74_09605"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013624748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK25623.1"
                     /translation="MTKVTAFEQPPHPYWLASSPQTNYPRLEEDVTVDAAIIGGGITG
                     ITCAYLLKKAGLTVAVVEADHLAQGATGHTTAKISSQHGLIYQKIKQQMGQAAAQQYA
                     EANETAIRQFEKIIKEEEIACDFSPQAAYVYTERDGYREKIEAEAELASKLGIEATVA
                     ETIALPFPVKAALRFAKQAQFHPRKFLLPLAESVPGNGSYIFEQSRIVGIDNDHNYLL
                     KTADGNRIHAEKVIIATHYPCYNKSGFYFTRIYQGRSYVVAVKAQQPYPGGMYITAEE
                     PGRSFRSLPTAEGELIMVGGEHHKAGQGADTREHYQALAQSAGEIFPQSAICCRWSTQ
                     DCITLDGLPYIGQFLPSTPNLYLATGYQRWGMTNSMVAAMLLRDLITKGESPWQDVYN
                     PSRLTLAASAKPFVTVNLNVANQLIKGKLATAPTEVELKPGEAKVMMVNDDKVGAYRD
                     QQGTLHLVDTSCTHLGCELQWNAAELSWDCPCHGSRFTYKGEIIEGPAIKALKAGQEH
                     RINRKEKSLT"
     gene            complement(13452..13661)
                     /locus_tag="VFC74_09610"
     CDS             complement(13452..13661)
                     /locus_tag="VFC74_09610"
                     /inference="COORDINATES: protein motif:HMM:NF019751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type small acid-soluble spore protein"
                     /protein_id="HZK25624.1"
                     /translation="MDNQRAEQIIKASEKIEVQYQDVPVWIENVQGSTADISIIGTNR
                     KITVPLAELEDTGRPISEQNIYGLQ"
     gene            14156..15520
                     /locus_tag="VFC74_09615"
     CDS             14156..15520
                     /locus_tag="VFC74_09615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZK25625.1"
                     /translation="MSTKEPLSNKVINLFSIGLSPFMLFMGPVMAYYPYFLTDIAKIS
                     ATTVGVILVVTRIVDAFAIIFVGGTIERSNLKWGKYVSWIYIGAPLTLVFFALMWVNP
                     GGMGMAAKAIYFTVIYIVGHIFMNFAWSGASSLIPSMTANPMDRVKLSARRTQFQTVT
                     MLIYSASHMPIIMYFGRNNEGTGYLTFMLIALVIQLVGYLLSAKIAKPYDTTTASGAA
                     VPSIGDMLKGIVTNKPLLILMLYGLFNFSVLSLANGLNIYVFKYVHNNVLLISLFLTV
                     NSLVNLASSFIGQFIGQRVSSKRTVMMIGTAVAMFSFLGAFFFARNTIVFMIFRWISA
                     LSGMGMVVNFAVYADAADYAEWKTGKSAKALIMSVSSLPAKTSGALVGAVTGFGLAAI
                     GYTADAAATPELVQGLIYLSTLLPVACAFIALIALSFYKLDNATMTKINDELTVRRAA
                     AAKQ"
     gene            15723..16613
                     /gene="era"
                     /locus_tag="VFC74_09620"
     CDS             15723..16613
                     /gene="era"
                     /locus_tag="VFC74_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HZK25626.1"
                     /translation="MYRSGFIALVGRPNVGKSTLMNALVGEKMAIISDKPQTTRNQIR
                     GILTTEDYQMIFLDTPGIHKPHHKLGEKMVQISLRTLREVDLILFLTDAAAGPGRGDE
                     FVIQKMQAVDTPVILLVNKADRSGPEEAQHLIEHYRPMYPFVGALAISALEGTNLAGL
                     TELMLKHMAEGPQYYPEDMITDQPERLIVSELIREKVLQFTREEVPHAVAVEISSMSA
                     RSDRDLVDIQANIYVERDSQKGIVIGKGGSMLKEIGKLARLDIEAFLGSAVNLQLWVK
                     VKNDWRNKEGTWQALGLELE"
     gene            16625..17368
                     /gene="recO"
                     /locus_tag="VFC74_09625"
     CDS             16625..17368
                     /gene="recO"
                     /locus_tag="VFC74_09625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HZK25627.1"
                     /translation="MTELRCQAVVLRVRSFKEADKLVTLLTRDAGLITAVARGSRKTK
                     SKFSALVEPLTLGHFLLYQGRTLYTFIQGEIIKTYRSLQGDLARFAYAQYFCELCERS
                     LLEGMPSEAIYMLLVTALEALEQDHDPARVARCFELSLLVELGISPILDGCSHCGSTS
                     GPYHFDPTEGVLSCRACPQSVESFPVSAATIAITKRLLEMGFHKLSVCVIPPTESKEI
                     HRLAADLLQYSLGIGKLKALDFLQKTGLW"
     gene            17455..17871
                     /locus_tag="VFC74_09630"
     CDS             17455..17871
                     /locus_tag="VFC74_09630"
                     /inference="COORDINATES: protein motif:HMM:NF025601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HZK25628.1"
                     /translation="MEITLQKIEQVRERSGLNYTEARELLEEAKGDVVQALVILENEE
                     GDLGMDMEMDEMKGMVSHSVVDPVKRVFHKSNRTRIRVKNEDGTLLEIPATLGIAGAL
                     LAPRATAISTMALLMAQYSLEVDVPEYVALDEPEWT"
     gene            18255..19148
                     /gene="glyQ"
                     /locus_tag="VFC74_09635"
     CDS             18255..19148
                     /gene="glyQ"
                     /locus_tag="VFC74_09635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015758492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HZK25629.1"
                     /translation="MDFQTMILKLQNYWARQNCVLAQPYDVEKGAGTMNPTTFLRALG
                     PEPWAVAYVEPSRRPTDGRYGENPNRLYQHHQFQVIMKPAPDEIQALYLDSLRELGIV
                     PEEHDIRFVEDNWESPTLGAWGLGWEVWLDGMEITQFTYFQQVGSIDVDAVSSEITYG
                     LERIAMYLQNKENIYDLAWVDGVITYGEIFHRAEVEHSCYSFEEADIEMLFTLFAMYE
                     KEGQRLLKKGLILPGYDQVLKCSHTFNVLDARGAISVAERTAYIGRVRHLARLAAQGY
                     LQQREALGYPLLRAERSAKGA"
     gene            19141..21228
                     /gene="glyS"
                     /locus_tag="VFC74_09640"
     CDS             19141..21228
                     /gene="glyS"
                     /locus_tag="VFC74_09640"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HZK25630.1"
                     /translation="MRKESFLLEIGTEEIPARFISPALAQLAEAAAAEMKQQRLAYED
                     IKVYGTPRRLALLVSKVAEKQQTLEEKKKGPAVKAAYDAHGKPTPAAQGFARSQGVAV
                     EELFTADVSGVPYVFAVRREAAHDVQTILPQLCARLIRSLNFPKPMFWHSKDVRFARP
                     IRWLTALWGSTPIPFSFAGLTAANVTYGHRFLAAEAVKLKEAADYPQVMQQNFVMAEP
                     QERKTKIAALVKEAARALGGHSVLHEDLLEEVVNLVEYPRVITGEFASAYLEVPQEVL
                     ITAMRTHQRYFPVFGEDNKLLPNFITISNGTSEEYAKNVRAGNERVLRARLADARFFF
                     EEDRKQPLAAYVDKLDKIVFMEALGTMRQKTARTIKLVQRLAEELKVDASVREAAERA
                     TFLAKADLMTQMVSEFPELQGTMGMHYARLSGESAAVATAIDEHYAPRFAGDTPPATL
                     PGALVALADKMDTLAACFALGLIPTGSQDPYALRRSAAGVVVTLADHSLPITIERLSE
                     LALSELQANLTQPAAEVTAQLADFMLQRVRYTFVEQGLRYDVIEAVLAGAVHNLPALW
                     QRAQVLQDKLNSPELTQILIPFTRVANLSRSEHSEVVQPKLLTAVAEKELYQALLTAE
                     KSVQAAAGRSDFTAVFTALAPLYQPIENFFADVMVMAEDVTVRQNRLALLAQIKKLFL
                     TLGDISTIVPEKK"
     gene            21334..21957
                     /locus_tag="VFC74_09645"
     CDS             21334..21957
                     /locus_tag="VFC74_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HZK25631.1"
                     /translation="MELNNRQKEILAIVKQDGPISGEEIAGRLNLSRAALRPHLAVLT
                     TSGLLDARPRVGYFYTGNVLTGISEILTRFTVGDIKSRPIVIREETSVYDAIVTLFLD
                     DVGTLFVVDEKGNLQGVVSRKDFLKTALGGVDLHKMPVGVIMTRMPQVHTVTEEETVL
                     EVVKRISEHEVDSLPVVRIQDSQTYKVVGRVTKTNIARLLLELSEGR"
     gene            21983..22804
                     /locus_tag="VFC74_09650"
     CDS             21983..22804
                     /locus_tag="VFC74_09650"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HZK25632.1"
                     /translation="MKRKPIVYVVSDSIGETAEFVVQAVASQFNSGHVEIRRIPFVTD
                     LETIVDVVEEASLGNAMIAYTLVLPSLREAIREESDLHGLIAVDVMGPMMDAFRRLMQ
                     LDPHQEPGLIRRLDEEYFRRVAAIEFAVKYDDGKDPRGMLLADVVLVGVSRTSKTPLS
                     MYLANKRLKVANLPLVPEVEPPEELFWVPASKVIGLTINPRQLNEIRQERLKALGLHA
                     QANYASMERILEELDYAEKIMRKIRCPIFDVSNKAVEEVANKILQLVREEARDGE"
     gene            22794..25439
                     /gene="ppdK"
                     /locus_tag="VFC74_09655"
     CDS             22794..25439
                     /gene="ppdK"
                     /locus_tag="VFC74_09655"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HZK25633.1"
                     /translation="MENKHVYLFKEGNAQMRELLGGKGANLAEMTKIGLPVPQGFTVS
                     TNACLEYLKRGEQTIWDELREEVMAALQTLEVISGKKFGDKENPLLVSVRSGAVISMP
                     GMMDTVLNLGLNDESVLGLAVAANDERFAYDCYRRFIQMYADVVQNIDHYNFEQKLEL
                     KKQSLGVKFDTELDAAALKELVEEYKAIVLKETKKHFPQEPVEQLILAMQAVFASWNN
                     QRAIIYRQIHKISDSLGTAVNVQMMVFGNLGDDSGTGVAFTRNPADGTNKLYGEFLVN
                     AQGEDVVAGIRTPLAISNMAEYLPEAYQEFTKVCDLLEKHYRDVQDIEFTVERGKLFL
                     LQTRSGKRTAAAAIKIAVNMAEEGLITKDEALLRVDPAQLNQLLHRRIDQDAKPEPIA
                     KGLPASPGAACGEIVFTADEADRLAGEGVKVILLRPETTPDDIHGIVAAQGVLTSRGG
                     MTSHAAVVARGMGKCCVSGCEEIKIDLLAKTMTAGEHVFKERDLISLDGSNGEVYAGT
                     VKLIAPELTDEFNTLLAWTDDVKDLGIRANADTPEDARKAREFGATGIGLCRTEHMFM
                     AQDRLPVVQKMILAKNEAERENALAELLPMQQGDFEGIFRAMDGLPVTIRLLDPPLHE
                     FLPNQEDLLVDVTLLKERGNDPQQLREKEELLKQVRGLHEINPMLGHRGCRLGIVTPA
                     IYRMQALAIFRAVVALIAEGKTIIPEVMIPLVGLETELELMRALVVQAADDVMAEAGI
                     NFDYKVGTMIELPRACMTADKIAAHADFFSFGTNDLTQTTFGYSRDDAEGKFLLDYLQ
                     QKVLPENPFAELDREGVGALVKIAVEKGRAVKPGLKLGICGEHGGDPASIEFCHLVGL
                     NYVSCSPFRVPIARLAAAQAKLKNK"
     gene            25610..26092
                     /locus_tag="VFC74_09660"
     CDS             25610..26092
                     /locus_tag="VFC74_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25634.1"
                     /translation="MIAVVYFSRDNNTKAGAEFLAASLEGTLVSLVEEKRSSFMGGAW
                     KALRGKPVKLKGEPWQEVQAVENIYLMTPIWANNGAPAINEFLLKTDFSGKKVTIITF
                     QADASFKGSELAHQTIAALIEKKGGMVLACHACHGARPGAFAGKEYIERQVAQVTLQQ
                     "
     gene            complement(26149..27297)
                     /locus_tag="VFC74_09665"
     CDS             complement(26149..27297)
                     /locus_tag="VFC74_09665"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HZK25635.1"
                     /translation="MTKKRVLSLLLAILLFLLSVPAAAQDFNTVAGVAALMEASTGQL
                     LYLKNADEPRPPASITKVMTLLLSFEAIESGEIQWDTTTIVSERAWQKEGSSMFLQVD
                     TEVSVEELISGMSIVSANDGCIAMAELLAGSEEAFVQRMNQRAQELGMTNTQFQNATG
                     LPAENHYMSARDIAILARELIVKHPKILEFESQREYTYNNIKQQNRNPLLGRFQGADG
                     LKTGWTPESGYSLAGTAEQNGMRLITVTLNTGSDQERLIASQELLNYGFRNFVFTQPA
                     AKDDALGETPVKDGKKQTVALTVTEDLIVLAPPNRAGDLKLVVAEEKTLQAPVEQGSQ
                     AATLLVQLDGETIASKPLVTATKVPRANIFVRFFRSIANFIRGLIKRA"
     gene            27493..28197
                     /locus_tag="VFC74_09670"
     CDS             27493..28197
                     /locus_tag="VFC74_09670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006873869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK25636.1"
                     /translation="MSEGLILVVDDEEHIRELVRLYLEKAGFAAALAADGEEAIQKVK
                     SLVPQLIILDIMLPKMDGWDVCREVRKIAETPIIMLTAKGEEFDKVLGLELGADDYLT
                     KPFSPRELVARVKAILRRVVVDDIAANEDIISLPGLNINQSSHEVHVREREVNLTPKE
                     FDLLWYLAKNTGKVFTREQLLTAVWGYDYYGDLRTVDTHIKRLREKVEREGTGTNAYQ
                     IKTIWGVGYKFEVQDA"
     gene            28194..29954
                     /locus_tag="VFC74_09675"
     CDS             28194..29954
                     /locus_tag="VFC74_09675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25637.1"
                     /translation="MKLKIFSKLLTTYLVIILITLFVVGLALTQLLQNYFYHAREGEL
                     QAKGQELADIYAAQMLGFQDPRVTSDLLFALDRFLDARVQLVDRSMLGLATCPGFEDL
                     GLPLTTAELDLLLNGHTITKRGYHSQSKLQMATVVVPVAARQEVLGAIFLHAPLTGIT
                     QTVKQVQELIFYAAILANILAMIVGYYMSKSISHPLQQMTRAALEVAGGNYQQQVEVP
                     SADEVGQLAQSFNHMSATLYQTVEALSQEKNKLENIMVSMNEGVLAIDQDAQVLLANP
                     QARELLQLESLDLAGQKITQLLPHAAMDELFMAVITKGEGQVQEYAVDGGRILSLQIA
                     PLQRGKKLWGAVGILQDVTEVRQLEEMRRNFVANVSHELRTPMTSIQGYVEALLDGLA
                     EDKESQQRYLQVILDETIRLNRLVNDLLDLSRLETRQLAWPLEPIDLRPLLEEVSAKL
                     QPQFAQLELTTALEVPVQLPLVVGNRDRIQQVLINLLSNAISFTPAGGTVTLAAAEAG
                     KWVRVSVQDTGLGIPAAEQEMVWQRFHKVDKARTRKFGGTGLGLSIVKQIVEAHGGSV
                     GLESAVGQGSTFFFTIPLAH"
     gene            30629..32416
                     /gene="dnaG"
                     /locus_tag="VFC74_09680"
     CDS             30629..32416
                     /gene="dnaG"
                     /locus_tag="VFC74_09680"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HZK25638.1"
                     /translation="MQKLPTEFIDELRTHYDIVELIAQYVQLKRSGRNYFGLCPFHAE
                     KTPSFSVSQDKQIFHCFGCGEGGNIYSFLMKIEGLTFPEAVRQLAERAGIPLPQIASS
                     PVLQKAELAKNRLRQIMQLANRYFQYKLQQPGGEAARQYLQTRGVTEAARENFALGWT
                     PDAWRELKTFLLRKGYQEKELLTSGLIIANKNTAYDRFRGRIIYPIANPQGEVIAFGG
                     RALGDEQPKYLNSPETPIFDKSKTLYALHLARDAIRRNEQAIIFEGYMDVIAAHQAGI
                     RQAVASLGTSLTESQARLLRNQAKEVVIVYDADAAGQAATWRGLQILREAGCLVKVGI
                     LPQGLDPDDYLRRFSGEAFQKEVIEQALLLVDYQLNSLAEQYNIEKDDERIRLFVKII
                     DVLAAVDNAMEREDYVQKAANLLQLPPTAIREELAKKKQPVTRPQQQRKSWPKQAATE
                     KAPLQILVLWARFPFLIAETAANLDSESIPEELREVFTAAQITAAFSPALLLDLLPSQ
                     KYRQLISRLLIEDDYDEKTASKALADCVKYLKCVHIAQQRKEVEAQMAKLDAVAAKGE
                     ITELSRKWLELRKMEESINQAKEGGTGVG"
     gene            32409..33542
                     /gene="rpoD"
                     /locus_tag="VFC74_09685"
     CDS             32409..33542
                     /gene="rpoD"
                     /locus_tag="VFC74_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009168776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HZK25639.1"
                     /translation="MDKKVTQKTENDKNQQLKKIREQLIIKGKKSGLLTYKEIIEALQ
                     ELDLSPEDIDDFYDHLSHLGVDVVDDTSEEEGVEPPEDDDKNEDSSETVTKITAAEGI
                     SVNDPVRMYLKEIGKIPLLTAEQEIELALRMEQGEEEAKRALAEANLRLVVSIAKKYV
                     GRGMLFLDLIQEGNLGLIKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIP
                     VHMVETINKLIRISRQLVQELGREPNPDEIAEVMNITEDRVREILKIAQEPVSLETPI
                     GEEDDSHLGDFIEDQDVQAPAEAAAFELLKEQLDDVLETLTPREKRVLTLRFGLEDGR
                     SRTLEEVGQVFGVTRERIRQIEAKALRKLRHPMRSKRLKDYLE"
     gene            33627..33701
                     /locus_tag="VFC74_09690"
     tRNA            33627..33701
                     /locus_tag="VFC74_09690"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33659..33661,aa:Asn,seq:gtt)
     gene            33703..33778
                     /locus_tag="VFC74_09695"
     tRNA            33703..33778
                     /locus_tag="VFC74_09695"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33736..33738,aa:Ile,seq:cat)
     gene            33790..33864
                     /locus_tag="VFC74_09700"
     tRNA            33790..33864
                     /locus_tag="VFC74_09700"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33823..33825,aa:Glu,seq:ttc)
     gene            33870..33945
                     /locus_tag="VFC74_09705"
     tRNA            33870..33945
                     /locus_tag="VFC74_09705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:33903..33905,aa:Val,seq:tac)
     gene            34135..34596
                     /locus_tag="VFC74_09710"
     CDS             34135..34596
                     /locus_tag="VFC74_09710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25640.1"
                     /translation="MWLLLIMLFLSPFIVIGYLVARLDRFLAGGGIVKDEVEVHPAAI
                     VFGQSSLAQEVTKILHKNKVRVFTIQEPFILAHKDNVQFLFALSENDADNLVLCKIGK
                     RIYGIEKAICLCNEQINKRLFGSEKISFLSGEEATACLFYQTVLHKAEVKP"
     gene            34593..35951
                     /locus_tag="VFC74_09715"
     CDS             34593..35951
                     /locus_tag="VFC74_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003413851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK25641.1"
                     /translation="MRELQLKLKLKSFTYPQMIALGYLIVITIGTLLLSLPLANYNHQ
                     SPGIFNALFTATSATCVTGLIVFDTYTQWSLFGQLVILLLIQIGGLGFMTIITMFSFL
                     LKRKIGLKERGLLRECVNTIYIGGVVRLTKRILLGTLLFEGLGAVILAIRFIPKMGIA
                     AGIYNGIFHAVSAFCNAGFDLMGRYGQYSSLTAYASDAVVCFTIMLLIIIGGIGFFVW
                     DDLAKNKFYVNRYQLHTKIVLAATISLIMAGSLSFFLLERNNLFAHLGVGEQIIASFF
                     AAVTPRTAGFNTVEVAALAPASKLLTMVLMFIGGSPGSTAGGIKTTTFAVILISLWSS
                     LKNARSENIFGRRLEENALRRASAVVSFNLLLVFSAVLLISATNVSVPLSNVLFEVLS
                     ALGTVGLSVGATSIFNPGARLVLVLLMYCGRVGSISFALLFTEHLKPSSVQKPTAKIS
                     IG"
     gene            35961..36614
                     /locus_tag="VFC74_09720"
     CDS             35961..36614
                     /locus_tag="VFC74_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011838176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZK25642.1"
                     /translation="MKSMLVIGIGRFGKHLSRKLIELSNEVMIVDKEEEKLTELLPLV
                     TSAQIGDCTNEEVLRSLGISNFDVCFVCVGSNFQNSLEITSLLKDLGARHVISKASRD
                     IHVKFLLRNGADEVADPERDIAEKLAVRYSANNVFDYIQLTKDVCIIEIPPLDKWVGR
                     SIKEVNFRVRYQVNILATKVGEMVLPLPSADHIFKADEHLMVLGHRSDVEKILKLLN"
     gene            36642..38033
                     /locus_tag="VFC74_09725"
     CDS             36642..38033
                     /locus_tag="VFC74_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009002884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4118 domain-containing protein"
                     /protein_id="HZK25643.1"
                     /translation="MRVKYYLRLCAIIIGTTIASLALSQLGVNKENILMVFIVGVLLV
                     TAVTNGYHYGIVASCVSVMIFNYFFTQPVHTFAISNTNDLALIFFFLSASVISSSLTA
                     RFQRQLLISQKNEETAKLLYDVAQRLLNVTGESNIIQQGMRCIKEYTGDDSKVELNGN
                     NQTYYSPNFAPAQNEKSIELPIMGLAKRLGTLMVYNVKLTIKQEWLVKTVCAQMGIAL
                     DREFVYNERETIRIAMEREHLKSNLLRSISHDLRTPLTGIAGASSYLVQRGSSLERDD
                     YERLVKDINEQAVWMTTLVENMLNMTRIDNGKLEVKKEIEVVDDVVGEAISHVTGLDE
                     RPFKVTLPQELVALPMDGKMIVQVLINLLNNAVTHTPKGTPIELSVKQGESYVEFSVA
                     DGGKGLAYLSVDKIFNSFVTSGKVGADGKKGIGLGLAICKAIVQSHGGSIQAGKSELG
                     GALFSFILPTEVE"
     gene            38033..38728
                     /locus_tag="VFC74_09730"
     CDS             38033..38728
                     /locus_tag="VFC74_09730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HZK25644.1"
                     /translation="MTEHTTVLVVEDDKYIKSLVTMLLKDEGYKTFTAATGKEAIALF
                     YANNPDITLLDLGLPDIDGMEVISQIREKSNAPIIVISAREEEIDKTSALYCGADDYM
                     TKPFYPGELVARIRVAQRKMNNAINPDNVEQFTFDNLTVDYTKGLVFVDEQEVHLTPM
                     EYKLLRILIANRGKVLTHNFIIKQIWGYGEAGDAKNLRVFMASLRRKIEKDTANPRFI
                     LTQVGIGYRFANK"
     gene            38852..39211
                     /locus_tag="VFC74_09735"
     CDS             38852..39211
                     /locus_tag="VFC74_09735"
                     /inference="COORDINATES: protein motif:HMM:NF022171.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HZK25645.1"
                     /translation="MRTIPALIVKYVLTLAAAYLASYFAGGVYWGLIIVLALLAAIAN
                     YVLADLIVLPVASNLVASLVDGGVAALLGFFMLARDTSLNLNTVWFVFTFALIVTGCE
                     YFFHIYLQTQEKSTNSE"
     gene            39660..41042
                     /locus_tag="VFC74_09740"
     CDS             39660..41042
                     /locus_tag="VFC74_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25646.1"
                     /translation="MTDTKALKEERISIYHDVADNKIPKRVPIGVSIGFEATMQYFGY
                     DLRNIYWNPSLLNKDEQKADELCQKVYSDTLPFSTSSLRFPTLYQFIESQSFVMSSSG
                     FMQHPEVVGMLPEDYDYLIEKPWDCILERIIPRHHKAFNTDEPIKMALNFTKGILGYL
                     NDPREGAALSATMRERYGYYPGAPAGSGGFTAMPFDFLADQLRSFSGISTDVRRRPEQ
                     VAEACEALYPIVFRRGLPKVVSNYGTVSIPLHMPTFMREKDFEKLWWPTAKRLVEEYA
                     SMGIHCNLFCEDDWMRYLDHLSELPTDTTLRFEFGDPKLVQEKLGDKFIISGLYPMQL
                     IKNGTKQQVIDKAKEVVDALAPGGKYTFGFDKGIQVGADLNVENLAALTEFLQEYAVY
                     DNPGETAGKQFNKNDYKAPTAASYKIDSKYFSTWEEYKAQYPNISDFAQDKIENYENQ
                     MLTLLTWLLM"
     gene            41239..42549
                     /locus_tag="VFC74_09745"
     CDS             41239..42549
                     /locus_tag="VFC74_09745"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HZK25647.1"
                     /translation="MSFLTYGVGDNKRWYYPMSAFMMGAMRIAPWALFYPHVQTAFNL
                     ETTANIILVSTFTGLGSMILGPPISGTILDKYGPKIPFALSGIFSLAGYLTLMVVLGK
                     ETWAEAQTLWYVGGFLIGLGAGFYAGSYTATVAKWFPDRLGVAMALGGSGMSIGTMIL
                     TPLIASFIVRYGFTGNIFMILGIVGFCAITLLGAIPWRLPKADWKPEGLGAKAKASGK
                     PVQEQKQFTFAEAVRDRRYWILAGGFLGAAFSNMLFSQNVTLIIIEGLTSVGMGRADI
                     MATVVPYYLSLTSFAGIFGTFLWGWVLDKLGPFVALPLMYFISGVLIWVFYMGYTSVT
                     FIYVVGAILVLAMRGEGTLHYAAVPAVFGRQHIGKIMSTLNSMSVGIGIALGPFLGSY
                     IRDITGGFKTSLILAVSLRMMGTAFALVGYFLTKKEKAKKDVTA"
     gene            42788..44716
                     /locus_tag="VFC74_09750"
     CDS             42788..44716
                     /locus_tag="VFC74_09750"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HZK25648.1"
                     /translation="MSLDSLRKHVNRVKQEWENFFAGKEVDGEVIPNVIKRSWVRSRA
                     YGVDPLSTLNDSWEPEIKWAAESNPLLQLNVNFTNPYDSAGYIIQLYDAAGYPLINSK
                     ATAPMIEKRMGTNSVWLALTQNKLVSVFGYEHYRASYHNTYGAAAPIHGNDGQVLGVI
                     NVVCNELELAPNIASMASQFAGVVEISLSLLNRNGKQSPLEAFFYQFPQGIAWYSENK
                     LIRYNQKILDMLQINEQAGIQAALEKQLSIWLSGRLINRKSVSMNILGQKKEFYFTSM
                     PIATGPGRGKEKFLLLEEKGNPKGSQQLVQHGTTLVEFDSIIGESKQLEATKRTAQKV
                     ASADEPVLIIGESGTGKELFAQAMHRASMRSGKFVAVNCGAIPAELAESELFGYEHGA
                     FTGALRNGKEGLLESASGGTIFFDEIESMPLFIQVKLLRAIATGRIMKVGSSQETPID
                     IRVISATKKDLLKEADAGNFREDLYYRLSTFILHLPALNERGNDIILLAQHFIKKTQQ
                     KYGKYPLKITRDFWRALTCYHWRGNVRELENIMGRVVFMAEQQTITAAALPAPIQESL
                     KLQPVQAALAESADLEEHADGLLQTGEEKIIAAVLQKTNFNISQASQILGISRKTLYN
                     RINSSERLSDLKSLKAKV"
     gene            44951..45589
                     /locus_tag="VFC74_09755"
     CDS             44951..45589
                     /locus_tag="VFC74_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014184967.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25649.1"
                     /translation="MLDLKVLTEAVGELDEEKVVSELEAFVASNPTEQEAQKAVAACQ
                     AGMAIVGDLFEQGEYFVGDLIFAGELLTNAINVLKPVLGSGTTDSIGTLVLGTVAGDL
                     HDIGKNIYKSMMEAAGFEVHDLGIDQPASAFIEKIKEVKPDVVGMSGVLTLALESMKE
                     TVDALKEAGVRDSVKIIIGGNPVTKEASEQIGADAFTTNAAEGVKIAQGWVS"
     gene            45846..46559
                     /locus_tag="VFC74_09760"
     CDS             45846..46559
                     /locus_tag="VFC74_09760"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HZK25650.1"
                     /translation="MKLTPRLQAIAELIPPGSVIADIGTDHAYLPIYLLLEQISQSAV
                     AADINEAPLEQAQETVAAFNCHQKIDLRLGDGLEILQEADGVDTIVIAGLGGRTIASI
                     LETGSDKLRQLKRIILQPMNETGYLRLFLAKNGLAIVHEELVLEGKHLYEIILAHPGQ
                     EIEEDPFKLSLGPRLLEKKPPLLPLLLKEKIRKLRIVYHSLQRAKKKDVSGKIRDVQK
                     ELDCLQEVLAGATQRTDTD"
     gene            46531..47649
                     /locus_tag="VFC74_09765"
     CDS             46531..47649
                     /locus_tag="VFC74_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HZK25651.1"
                     /translation="MQLNVQTLIKILEELAPKHLALEWDHVGLQLGSMQGTVKKIYVA
                     LDVNAEVLAEAVMKGADFIVTHHPFIFKPLTTIRTDQEKGKLIQQALQTGIRIYAAHT
                     NLDIAFGGVNDVLAARLGLQGTQVLRITDQEELLKVVVFVPQRDEHNVRTAMAAAGAG
                     CWGNYSHCSFYTTGTGTFMPQKGSNPYLGEQGQLEKVEEVRLETIIPASLSKRVVRAM
                     LKAHPYEEVAYDLYPLANKGKEYGLGRIGNLQHSLLLSEFCHLVKERLEIPFVRVTGD
                     TDKLISKVAVCGGAGSDLIAAASFAGAEALVTGDLKYHEAQEAQVLGVAVIDAGHDGT
                     ERIIVPALCDYLQQQLAAAGYHIEIIASSIDTTLWNLF"
     gene            47798..48505
                     /locus_tag="VFC74_09770"
     CDS             47798..48505
                     /locus_tag="VFC74_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25652.1"
                     /translation="MGELQKLYELQQVELKIESLQKTLKELPVFAAFKLLQAETADAK
                     EALGWAETKLTEQDKRLHRLEMDLQDAEAEQEKAQTQLFSNSNQSSKGLEQLERRSDA
                     LGKERLEKEEMLLLAMEGKDNLAQTLQKSKDDYRSRRKKLRQLQKTGNAEIQELKNKI
                     NDLQEQRKQLQEQISESLLKDYRQQRPRYNGKPLAHLENDCCSGCRVMVFKTLKSNLN
                     QPNSKVFCENCGRRLVP"
     gene            48583..48930
                     /gene="rnpB"
                     /locus_tag="VFC74_09775"
     ncRNA           48583..48930
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFC74_09775"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(49100..49462)
                     /locus_tag="VFC74_09780"
     CDS             complement(49100..49462)
                     /locus_tag="VFC74_09780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK25653.1"
                     /translation="MITELNNCYESLSSNNCPCKNYCPLGNALKLIGGKWKTPILCAL
                     HHDGTTRYNELKRKIRGITNTMLASSLKELVNDGLLYRKQYDGIPVRVEYSLTDACED
                     LMPILNLLAIWGVKMSQE"
     gene            49583..49888
                     /locus_tag="VFC74_09785"
     CDS             49583..49888
                     /locus_tag="VFC74_09785"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019225840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK25654.1"
                     /translation="MVINEEVKEVLEGSAFLTLVTVNVDGTPHPIIAGKGQVVNDTVV
                     FGIYKMVITQKNLMSNKSAWVVGATKAEGAKGYRLTGTAEAKDKQLIFTPTKIDVLL"
     gene            50261..51724
                     /gene="rmuC"
                     /locus_tag="VFC74_09790"
     CDS             50261..51724
                     /gene="rmuC"
                     /locus_tag="VFC74_09790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020375217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HZK25655.1"
                     /translation="MLETILLVFAILNLVVVLLMLQKMGHVDNRELKQMMREEIAALR
                     NEITLSARQNREELNASVRAFGDSLSKQSMEMAMLQKVQLDTLTASNEQRLDALRKSM
                     EAKLTHLQGQQNTDALQNRTELAKSLHSFEEKFHTSVNELNATQRQKFSDLAEQLEKL
                     VQTSADKLERMRGTLEAKLQELRTDNTEKLEMIRATVEEKLQATLEKRLGESFKQVSE
                     RLEQVHQGLGEMQNLATGVGDLKKALVNVKVRGTLGEIQLASILEEILTPEQYAVNVA
                     VRPGRNERVEFALKMPGKDEHPVWLPIDAKFPMEDYQRLLDAYESADLAQAEACAKQL
                     EASIKKCAKDIRDKYLDPPATTDFGIMFLPFEGLYAEVLRRNGLFETLMRDYKVAITG
                     PTTLAAFLNSLQMGFRTLTIEKRTSEVWSLLGAVKTEFGRFGALLDKTRKKLQEASNT
                     IDVASRKSRTIERKLKDVQELPDLESEQVLLPVEENS"
     gene            51857..53065
                     /locus_tag="VFC74_09795"
     CDS             51857..53065
                     /locus_tag="VFC74_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024423.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HZK25656.1"
                     /translation="MIKKELVKLLFDAASIQRWNDHNRPYKGFTELDKQAQKMVYAYL
                     LAKFEQSDHDVCVNWRQLIEGGFFEFLHRIILTDIKPPIFHKLMAEKGDLLNQWVITQ
                     LTEALAGVNDNFLEKFKAYLFQPEYSALEKSILKASHYLATNWEFKLVYTLNANLYGL
                     EETKATIEDEIEEHYHLAGVQKLQLGKKTSHFMDLVGQLRFQQRWAQTARLPETSVMG
                     HMLMVAMLSYLVSTELNTCDQRLVNNYLAGLFHDLPEVLTRDIVSPVKRSVVGLDELI
                     KDIEQRQVEEKLLPLLPAAWHKELRYFTEDEFANKVILDGKVTIVEGSTIGNQYNQDR
                     YSPLDGQLIRACDHFAAYMETYLSISHGIKSHFLEEGYQQLLLQYQDKSVSGFPFWQW
                     FNYFRHPETN"
     gene            53113..53919
                     /locus_tag="VFC74_09800"
     CDS             53113..53919
                     /locus_tag="VFC74_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007063604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HZK25657.1"
                     /translation="MSNESFNLEAYFKRINYQGKTDVTAETLKGLHIAHTLNVPFENL
                     DVHFKKPIRLDRESLYQKIVENKRGGYCFEMNGMFSFVLKEMGFKVLDVLARGTRDGK
                     EFFSQSHQVMVVEIGDELWLADVGFGNDGITAPVLLEEGVDQQQFTHTYRILKDPKHG
                     YILQSKVDGEYKYMYAFHLINCHPMDFEMANYFMATYPNSFFSIMKMCTMPTKEGRVT
                     LTDIRNEKKFKQLVNGQVTETNLNSEDEFKEKLQQYFALDYEAIKQSAAK"
     gene            complement(54056..56635)
                     /gene="clpB"
                     /locus_tag="VFC74_09805"
     CDS             complement(54056..56635)
                     /gene="clpB"
                     /locus_tag="VFC74_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HZK25658.1"
                     /translation="MDLNKFTEKAQLALSGAQDLAVQRSHQALDVEHVLLALLLQENG
                     LIPRLLTKADTSVSNLQQVLEQTLSKMPAVTGAAATYISQRLNQLLVQAQEEAKRLKD
                     EYVSVEHLVLAMFDDTAMTKIFKKQGLTRDTFLQALTAVRGNQRVTSANPEETYEALE
                     KYGRDLTTMAAQNKLDPVIGRDQEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIVEGLA
                     RRIVAGDVPESLKDRRLVALDMGSLIAGAKFRGEFEERLKAVLEEVTKSAGRIILFID
                     ELHTVVGAGKAEGAMDAGNMLKPMLARGELHCIGATTLDEYRQHIEKDAALERRFQPV
                     LVEEPSVESTISILRGLRERYELHHGVRIQDAALVSAALLSHRYISDRFLPDKAIDLI
                     DEAAAKLRTEMESMPEELEILERRVLQLEIEREALRKEEDQPSQERLSALEKELAELK
                     TTRDTLRAQWDNEKQAIGGLGALREELEKARTEMEQAQRDYDLNKVAEYQYGKIPALE
                     KKLQEAESKLVGSEAKLINEAVTTEDVAEVVARWTGIPLSRLVEGEKEKLLHLEEILH
                     KRVIGQEEAVQAVADAVLRARSGLKDPKKPIGSFIFLGPTGVGKTELARSLAEALFDS
                     EDNMIRLDMSEYMEKHTVARLIGAPPGYVGYEEGGQLTEAVRRKPYSVLLLDEVEKAH
                     HDVFNVLLQILDDGRLTDSQGRTVDFKNTIIIMTSNLGSIYLLENVTSSGEILDTVQE
                     QVMAELRSHFRPEFLNRVDDIVLFKPLTLAETKQIVELQLTLLRNRLAERYIGLELTE
                     AAKERLAQAGYDPVYGARPLKRYIQKEMETTLARRLIAGELTEHSIVLVDASDDGLTF
                     TVS"
     gene            complement(56931..58610)
                     /locus_tag="VFC74_09810"
     CDS             complement(56931..58610)
                     /locus_tag="VFC74_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015359763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HZK25659.1"
                     /translation="MTDIEQTTTIKIILPDQTSVTKEAGRSLLEISEEVAEHYASPIV
                     AAIVNDKMKDLTHLPQPSSVVKFLDLGTTEGARIYQRSLTFLLIYATRLLFPDAQISV
                     EHSLGKGLYCEINKTPALTTEDVAALQHKMRELVLADLPIEKKKIPLQQAIDCFKQAG
                     FDDKVRLYKNWPTEALTVYSLGALTDSLHGYMVTRTGILQWFAVHFRAPGLVLRFPNA
                     ADPFTIPPYIEQHKLFGIFRETEEWSEILGFHTVGILNERIEAGQGPEIMRINEALHE
                     KKIAQIADSIAARKDELRIVLIAGPSSSGKTTFTQRLRIQLLVNGIRPFPISLDDYFV
                     DREHTPLDENNQPDFEHLEAIDLELFNNHLLRLIDGEKIEIPRYNFHTGKREYHGHTI
                     ELQENQLLLIEGIHGLNERLTEAISRRHKYKIYISALTALNVDNHTRIHTTDTRLLRR
                     IIRDHQFRSYDALETIRRWPSVRRGEERNLFHLQEEADIMFNSSLVYELAILKQFATP
                     LLQEISHTEPEYIDAKRLLLFLSCFASLELQGIPNNSILREFIGGSCFHEI"
     gene            complement(58730..59698)
                     /locus_tag="VFC74_09815"
     CDS             complement(58730..59698)
                     /locus_tag="VFC74_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HZK25660.1"
                     /translation="MIKLPANLTPQYYAAEEQYKKASLPEDKVAALQEMLATIPKHKG
                     TEKLQADLKKRLSQMREESQQKKQQKGTYNPFHVEKQGAGQVVLTGCPNTGKSALVGA
                     LSRAKVKVGDYPYTTTIPAAGMMQYEDILIQLVDTPPFTNDNLPAGLIGTLRNADALL
                     LLIDLSTGDCLEQAETTLSLLEERGIIDPSGESPVALPYLTLGLKADLPDATSNLEIL
                     QELRPDIQIQPLSIDHDLTALRANIFMLLDVIRIYGKAPGKKADRERPFILKSGSTVL
                     DLAAQVHRDFPQKLKSALVWGSARFAGQAVPRDYLLQDKDIVELVL"
     gene            59865..60392
                     /locus_tag="VFC74_09820"
     CDS             59865..60392
                     /locus_tag="VFC74_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HZK25661.1"
                     /translation="MTAASKKSTVKVLVPVLLLALVFGVWYVKNAQSDKIPTGGVNPD
                     FALAVTEEFDLEHLKSYGLPIMLDFGSDDCAPCQAMAPVLEELNLELQGKAIIKFIDV
                     WKYEELGQDYPLTLIPTQVFIDAEGKPYTPSNPKAMEMTLFTHRETNEHLFTTHVGPM
                     TKEEILAALAEMGMK"
     gene            60392..61087
                     /locus_tag="VFC74_09825"
     CDS             60392..61087
                     /locus_tag="VFC74_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015261488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HZK25662.1"
                     /translation="MDSSQWLQSLATMIAAKMWLAPLFAFAAGVLTSFTPCALSSIPL
                     VISYVGSSGEGDTKNAFWLSAIFALGSAVTFTILGAAASLLGRLMQSVGSWWYLVLGV
                     LMLLMALQTWEIFSFIPSSYAVSRNKQRGFGGAFVAGILGGFFSSPCSTPVLVVLLAF
                     VAQEGSLLWGVVLLLLYSLGHSLLVLLAGTSIVFVQKLSRSDKYGKFSNILRILMGTL
                     MTLIALYMFYLGF"
     gene            61417..62799
                     /locus_tag="VFC74_09830"
     CDS             61417..62799
                     /locus_tag="VFC74_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HZK25663.1"
                     /translation="MQNKELVNGYLPDENPPVWQLFLYAIQQVIVMFPATVAVAIITG
                     FHISTTIFASGLATIAFVLVTGRKIPLYYGSSFSYVAAIGSLMASESLAGYPLNDKIA
                     IAQFGIIMSGFVSIAAGIVVNRFGRDAIEKILPASVTGPIAMVIGLTLAATALQDAAS
                     IAIDTAGTINVAEAQIALASNFAWTISLVTLLSTILFSVYLKGVWGQLPLLLGPVLGC
                     ITTYIIKLATGISLFKVLPAAANTSIFALPMFTLPKISWLAVAAIMPIAIATIPESTA
                     HVYQLDIYVNDLAKKKGSSKRYNIADKLGLNLIGDGIGDIVSGLIGGPAGTNYGENIS
                     AMAITRVFSVPVLIGASIIAMIIACFTPLINSIYAIPTAVIGGLEVYLFGAIAAQGIA
                     IMVEKSVDLFSSKNIAIIATIMVIGIGGQYGFGGNIPFFGLQVPTIAGAAIFGIILNL
                     LLSFGKKKDA"
     gene            62957..64882
                     /locus_tag="VFC74_09835"
     CDS             62957..64882
                     /locus_tag="VFC74_09835"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012281279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HZK25664.1"
                     /translation="MGENLTRKIIGEHLAAGEMQAGTEIALRIEQTLTQDATGTMAYL
                     QFEAMDVARVKTQLSVSYIDHNTLQTGFENADDHRYLQTVAAKYGIYFSRPGNGICHQ
                     VHLERFGVPGKTLLGSDSHTPTGGGLGMLAIGAGGLDVAVAMAGGPFYLTMPKVVQVK
                     LEGQLQPWVSAKDIILAVLRCLSVKGGVGKIIEYTGDGVKTLSVPERATITNMGAELG
                     ATSSIFPSDEVTRAFLAAQGREEQWVELLPDADAAYDETLTIDLSQLEPLVAAPHSPD
                     AVKTVQELGELPVAQVAIGSCTNSSYADLMQVAKIIKGKKVHQDVSLVISPGSRQVLT
                     MLAQNGALADLITAGARILESTCGPCIGMGQAPASDAVSVRTFNRNFLGRSGTASASV
                     YLASPEVAAATALTGRLTDPRTLGTYSPISQPEQFLIDDGMIIPPAASGEGVEVVRGP
                     NIKPLPVNSPLPTDLTAPVLLKVADNITTDHIMPAGAKVLPLRSNIPAISEFVFAAID
                     LDFTSRAKAAGKGMVIGGHNYGQGSSREHAALAPMYLGVKIVLAKSFARIHCANLVNF
                     GILPLTFCEEADYEALKQDDILSITALTTQLQAGNTITVTNDTSGVSFSVKHELTNRQ
                     IQIILAGGLLNFTRGQA"
     gene            65010..65738
                     /locus_tag="VFC74_09840"
     CDS             65010..65738
                     /locus_tag="VFC74_09840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005931734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK25665.1"
                     /translation="MKRAIIHIDEEKCNGCGLCIPSCAEGALQIVNGKAKLIADNLCD
                     GLGACLGDCPQGALIIEERDSDAFDEEAVEQFLAAQKEQTPPTSTPFIGCPGSRTRVL
                     NTPAPTETAVEGEEIPSALGHWPVQLNLVNPIAPYFKEADLLIAADCVPFAYADFHRQ
                     FLRGHALVIGCPKLDDAPLYVQKLTALIRSNNLKSITIVVMEVPCCGGLHGIIREAMR
                     RAECTVPVEVVQVSVDGKINFRSK"
     gene            65864..66931
                     /locus_tag="VFC74_09845"
     CDS             65864..66931
                     /locus_tag="VFC74_09845"
                     /inference="COORDINATES: protein motif:HMM:NF022233.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraprenyl-beta-curcumene synthase family
                     protein"
                     /protein_id="HZK25666.1"
                     /translation="MSSILENTHDYTLILPYVTRIFPLVDRELNVWLAKASTIPDPEL
                     RKQALASIDKKRFHCQGGSIYALYTREKSTELIHFIVALQTISDYLDNLCDRVPGASE
                     ESFRRLHQAILAAVDIEETCQDWYEHYPFCNDGGYLKQLVARTRETLVRLPGYQDVRT
                     TLRSLLQLYVDLQVYKHIEEPLRVERLVHWYSQNHSLASDVYWWEFSAASGSTLAVFL
                     LTAMAAAGPVSKSEIEQVLACYFPWLCGFHILLDYFIDLDEDQQHHDLNFVDCYPTPL
                     VAERGLLNFLSAALQKTAALHRPAFHRTVVYGLLALYLSDPKANVPERKKLSQQLLRT
                     GGNEALWLHRCCLLLRKLGKI"
     gene            complement(66928..67902)
                     /locus_tag="VFC74_09850"
     CDS             complement(66928..67902)
                     /locus_tag="VFC74_09850"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HZK25667.1"
                     /translation="MTIAKTPLMELQALPNLKLVEARLQELSRGTDLWTPQFSAAVTT
                     GGKRLRPALVLLCGSFYPVGQSKLVDAATAAELIHTASLVHDDIIDHASLRRGRPTIS
                     ATWGTQQAVLYGDFLFARAFSILTEHGLASILGKMTRAISLMCEGEIEQSSRLFNCQL
                     QEDDYFDYIYKKTAYFLSACCAAGAEVCGLPQEFSQCLATFGLQLGQAFQLIDDLLDI
                     TSDSKTTGKPVLQDLREGYLTLPLIKLVQHPDYGWRAQQIIAEKRFVPENLDFICQSL
                     HDSGIIKETYEKARALILQAKENLTQLPLKPARVLLARLADYVVSRSS"
     gene            68185..68874
                     /locus_tag="VFC74_09855"
     CDS             68185..68874
                     /locus_tag="VFC74_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25668.1"
                     /translation="MQNIKNNKPLVKVIVLLICGGLVLSSVAAAIISITEPQPTEDPS
                     AIYRSQLAQVETVIVQTEKSLEEKPGNVALLKQLGNAYYTAGQLQAALNEAEKSLESF
                     AQALEPYGKVLEQEPDNVEVLVDRAVAAYASENNEIAQEDFEKAIAVAPNHAKARFNY
                     GVFLYFGLAQPEEALAHWQKVVELNPEDEQELVATTKTWIKAVEAELAEQAAELEAET
                     NEEDSAKTDAE"
     gene            69084..69290
                     /locus_tag="VFC74_09860"
     CDS             69084..69290
                     /locus_tag="VFC74_09860"
                     /inference="COORDINATES: protein motif:HMM:NF019485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1657 domain-containing protein"
                     /protein_id="HZK25669.1"
                     /translation="MTVATQLKQTLVSLKGAQATLRLYEQLSRQQEVRHAYQQALQTV
                     EAVVGDLEVRTREIESQEPQYQGS"
     gene            69303..70163
                     /locus_tag="VFC74_09865"
     CDS             69303..70163
                     /locus_tag="VFC74_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004630645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HZK25670.1"
                     /translation="MPAWLMVLLRSSVLFVLVFFFIRVLGTKHPAKMTPFRFVNYAVI
                     AVIIGLLASTAIDSFILGLAALSVWLVMPFVLDYLELRSKAVHDLLEGKEAIIIKQGK
                     IMEENLKRARLTGEELLRDLRAKNIFSISDVEFAVLEANGELNVMLKSDQQPLTAHDL
                     AWQVTPQTEPQTVILDGNILNDPLTELGLNSDWLQTELVKAGVSLDNVFLGQVNSVGE
                     LYIDLFNDAIKVENPSVRQALFATLEKAQADLTSFSLETQQEQAKAMFARNAADLQSV
                     LDGLRPHLLR"
     gene            70179..70658
                     /gene="spoVAC"
                     /locus_tag="VFC74_09870"
     CDS             70179..70658
                     /gene="spoVAC"
                     /locus_tag="VFC74_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003446022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AC"
                     /protein_id="HZK25671.1"
                     /translation="MSNKKNKKLSAAQQEYQKFAKDREPKRPLLTNCLHAFIAGGLIC
                     TLGQFFQWFYMTYFNFTEITAGSPTQATLIIISALLTSLGVYDHLAQWAGAGTIVPVT
                     GFANTITSAAIEHRSEGYVLGVGGNMFKVAGPVITFGVFSAFVVAIIKIIIQKLGGI"
     gene            70660..71670
                     /gene="spoVAD"
                     /locus_tag="VFC74_09875"
     CDS             70660..71670
                     /gene="spoVAD"
                     /locus_tag="VFC74_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015757892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AD"
                     /protein_id="HZK25672.1"
                     /translation="MLQGHQSWVFDKHPVIISTGVAGGPFEAAGALADDFDILHDDTW
                     LGQESFEKAEKKLLEEACEKAIDKAGMKKEDVHFFLSGDLMNQIISSSFAARTLGIPY
                     LGLFGACSTSMAGLAVASFIINAGGASHVLAAASSHNSSVNKQYRYPTEYGAQNPPTA
                     QWTVTGAGAALVAAAGEGPRVTGATIGRIVDLGISDPFNMGAAMAPAAVDTIEAHFRD
                     FNVDASHYDLIATGDLGRVGHNIAGDLLIKHGVDIPEDKFTDCGLLIYKDNQPVIAGG
                     SGCACSATVTYGHLLNRMRRGEIKRILVVATGALLSPLSYQQKESIPGIAHAVVMERE
                     GD"
     gene            71672..72025
                     /gene="spoVAE"
                     /locus_tag="VFC74_09880"
     CDS             71672..72025
                     /gene="spoVAE"
                     /locus_tag="VFC74_09880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007061968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein AE"
                     /protein_id="HZK25673.1"
                     /translation="MEKYLIAFVIGGTICVIGQIMLDVFRLTPAHMTVTLVVTGAILG
                     GLGLYEPLVKFAGAGASVPISSFGNSLVKGALSEAKSTGLVGVLTGIFEVTSAGISSA
                     IIFGFLASLFFKPKG"
     gene            72055..72258
                     /locus_tag="VFC74_09885"
     CDS             72055..72258
                     /locus_tag="VFC74_09885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014185966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1657 domain-containing protein"
                     /protein_id="HZK25674.1"
                     /translation="MTVGTQLQQAIAGIQSAAASMKTFALETQDTQAQQTFNQLAQTL
                     DTAVDTLKQRQQYVQSQEPQYKE"
     gene            72483..73313
                     /locus_tag="VFC74_09890"
     CDS             72483..73313
                     /locus_tag="VFC74_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25675.1"
                     /translation="MKIAILSGKGGTGKTTVATSLVASLPACQYIDCDVEEPNGYIFL
                     NPEVTNTIAVNVRVPKVDQDKCSGCGSCAEACQFNALAVVKDKVLVFPEICHHCGACL
                     LACPEGAISEIDREIGVVEANADNTFLQGRLNIGEPLSIPVIQQLKKHLHGELPIILD
                     CPPGASCAVVQSIEDCDYCILVAEPTPFGLHDLQIAVRLVKLMQIPCGVVSNKATPGN
                     NTIQDFCAEHELELLMEIPFSREIAAAYSRGILPVTTCPEWQEKFCALYQKVEKGAQK
                     "
     gene            73310..74203
                     /locus_tag="VFC74_09895"
     CDS             73310..74203
                     /locus_tag="VFC74_09895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25676.1"
                     /translation="MKQIAFVSGKGGTGKSTLVSSLSLLVTDKMLADCDVDAPNLHLL
                     VAGNVIKEDYFFGAKEAIINAEKCQQCGLCSETCRFGAISDELEIIPLKCEGCGACVL
                     VCPHEAIRLEEVKTGRTYLTNTTQGTFSHALLDIGAEGSGRLVTEVKKNILESYKQEK
                     WLLIDGSPGIGCVVIASITGSDAVVAVTEPTKSGVSDLERVLGVAHHFQIPSFVCINK
                     YNLNLEITAQLEEYCQLHNCPVIGKIPFDESIVAALQAGKTPVEAGNNKVTQEIIQLW
                     QRLTAALAEEEPLLTSIGEEG"
     gene            74225..74692
                     /locus_tag="VFC74_09900"
     CDS             74225..74692
                     /locus_tag="VFC74_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010243367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HZK25677.1"
                     /translation="MVRPTKWRKVEFIPDHQLFRPMDIDVTLENVLQIEELEAIRLKD
                     LEGLDQEECAKRMEVSRPTFQRILSAARLKIADSLINAKAIRIAGGNYTRNICPVKCL
                     NCGKEWEESYENFEKVLSGKYNCPDCASTQVACARREKKKFCQGHCRRHGLQD"
     gene            complement(74783..75121)
                     /locus_tag="VFC74_09905"
     CDS             complement(74783..75121)
                     /locus_tag="VFC74_09905"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZK25678.1"
                     /translation="MIIAVPMVEDQLCLHFGHCEKFAFFDVDTTEKTIKGVKLLVPPP
                     HEPGLFPVWVREQGAELVITGGMGGHAQQLFQSAGVQVLTGAPAIKPEEVVKSFLQST
                     LKLSDNTCDH"
     gene            complement(75151..75513)
                     /locus_tag="VFC74_09910"
     CDS             complement(75151..75513)
                     /locus_tag="VFC74_09910"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HZK25679.1"
                     /translation="MKVAVSVQGTTLDDPMDERFGRAARFIIYDLEDETYKLLDNQQV
                     YNAPQGAGIQAAQNVINAGAEAIITGHVGPKAFRVLKSTDVSVHLARKITVQEAITSF
                     KEGTLETAETNDVEDHWV"
BASE COUNT        21110 a        15074 c        18119 g        21619 t
ORIGIN      
        1 agtatagtta ccccgaacat ttgccgatac tataatggat aagattatat aaaaaagtta
       61 aatgaaactt ggtaaaattc tgtaggaata ctgcgtgtat tcttgacgat cagccaattg
      121 atagagtaca attaacaagt aaaaagaata ttatggaata tattctggaa ggaggatctg
      181 catgagtcta caggcacggc aaattactga agatatgacg attgcacaag tcttacgcct
      241 taagcccaat tcggtaaagg tatttatgga agttggtatg cactgttttg ggtgaggtgc
      301 tgcaactggg gaaacagtgg cccaggccgc catggttcat ggcgtcgatg ttagtaaact
      361 aattgctgat ttaaataagc tacattaaaa accgcgcaag cggttttttt acttgtatac
      421 tttctaattg acattaatca tcacctgcag ctataaattg tcttttgctc tctggagatg
      481 acacgagaga atgctagagg gaatttaaga aaatattttt cactaatgtt agtttaacct
      541 ttgttttagt caagctgtgt ggtataatcg gaatagttag aatagttgga taatgcttgt
      601 tctagcgtta atgacaactg gtcgcctttt aattttggca aagcacataa aaatagtcgc
      661 atctaagtta aacagctagc atccgaacaa aaccttgtca tattgttaag ctattccttc
      721 ttaatgatta gggaagttag tatgtataag gggggacgca atgctaagct aataactgat
      781 tagatgactt gtcgttggac tttgaaaaat taaaaaggag cgtgagttat atgaaaaagc
      841 cgttaagccc agctatcaaa acattttatg ggtttggcga catgggcttt agcttgatgg
      901 cgtccgtaga aactttcttc tttgtttatt ttcttaccaa cgtggctaaa ttttccctgc
      961 ccatggtggc gattgtaggc accatcacta gtgtcgctga tgctgcttta tcacctttct
     1021 atggcggaat tattgatggc accaaaccaa tgaagtgggg acgtgttcga tcctggatgc
     1081 tgctttgccc acccattgtt gttcctcttt atatcttcca atacagcaat attggcgcta
     1141 acgaaactgt tgccgcggcc atcgtctgcg caggttctat cctcagccat atcttttgga
     1201 atctaccgtg ggttgcgaac ctttctttaa tttctgtctt agccaataat ccaggggaaa
     1261 gagcattctt atcatcaaga cgagcaactt ggacgatgat tgctggcatg ctgttctcct
     1321 ttattggttc gaccatggcg atcaagattg gtgaggtaac tgggaatgaa gctctgggtt
     1381 accaggcact ggcaggactg atggggcttg tcttcctcat tggttactgg acagttttca
     1441 aaataactga tggctatgaa gaaacaggtg ccgcagcaca ggcaagtgcc gccaagggca
     1501 atcaagtgtc tgttggcgtt atgttaaaaa gtgcgctgca gaatcctcct ttgctggtct
     1561 tattggtggg tgactttttc cgctatatgg tgaactttat tatgtcatcc tctgccgcct
     1621 actatttcac gtatgtggcc aaaaatatgg cactactgcc catttatttg ttcgtcggct
     1681 ctttaggcgg cgtaattggt gcttattttg cagggattct ctgtaataag tactccacaa
     1741 ggacagtttc aatctggggt ctctttgcga tggcgttatc cttaatcgcc tgcaagtttg
     1801 tggccttcaa tattatcctg ttctttggct tagtgttagt ggcacgaaca atgcttggcg
     1861 tgctatctgc ctcgatcgtg gccctgtact cggatgctgc tgtctatagt gagtggaaaa
     1921 ccggcaaaag tgctgcctca tttgtcatgg gtctaatgac gatatctctg aaagcagctg
     1981 tcatttcccg gggcacagta attccgtttg tgctggctac agctggcttt gtggcctcag
     2041 ctgaccccgc aactgcttct ttagaattgc aaaatgcggt tgtcaacgtt tttgtcttga
     2101 ttcctggtgt cttggctcta atttctgcct ttattattgg ccttggttat cgcttaacca
     2161 gagaacgcct tactgaatta caggccgaaa ttgatgcgag aaaagcagca acagctcaat
     2221 aagtattttt atacatgagg actgaaaaag gttgttaacc atgatcatgg ttaacaacct
     2281 tttttctttt ccgcaggatg tatgaacaag accttttctc ctaaaactaa agatagcaaa
     2341 ttttagggag agcaggaatt gatgaaaaat ctagaagaaa ttaagcagaa ggaaaaaagt
     2401 gtgcgtgaaa cacttttatt taaaacaaca gttttacttg atcaactact aacttatgtc
     2461 ccgacccgtt ctattcttgc tgtcaacgat atacctgtga aagtaatctg tggttatgat
     2521 ggtcaaaaat cgctgctgat taataagcgg ccgctttcta ctgcggatga ttgtgaatgg
     2581 gttattgaaa attacgaggc tttaaagcag gcggttgagg agttagtgaa tccagaagca
     2641 gaagaaattc aaaaagttat tgagcgagcg gaagaattaa tcgaaaaagt agataatatc
     2701 actaaaactt aaaacgaagc atctaggctg ctagaacgac attactcctg agggggtcgt
     2761 gtcgtttgtt gtttagatag gggacggtgg agacgcgttt ttatagtact taggtcctat
     2821 taataaagaa atagcaggaa aatatgacta attgtcgaaa aagaaaaaga ataaaaaaag
     2881 atgtgtagcc tgcagggggt aatatacgtg gaattagagc aaacaatatc aatggctatt
     2941 cccttgtttg catttttttt gtatactggc ttaatgatct tgagcattaa aagttcccaa
     3001 accaaagtta cgaggctctt tcaaaactat ctattattga tgatggattg gagtttaggc
     3061 tcttttatga tgcggacagg ctatgctccg gctcctttgt tttggaaccg gatgatggtt
     3121 gtcggcatga ccggagttcc ttttatcttt tatcgcttta gtgaagcgct cttaagcaga
     3181 gcgcaatctc taacctctaa aataggatat tttttatacg gacttttact cattgccaat
     3241 ttcaagggac ttcttgtgca ggatgtcatt ttaagcgcag gagatttaag ttatactatt
     3301 ggtcccttgg cagtaccatt tgcgctgata agcggcggct atgttttact agcaaccatt
     3361 ttattagtgg gtgagggctt caaaaaccca gcttctttct ggtcaaaccg tctgctttat
     3421 ccttccattg gtacagtttt gatgtttatc ggctgcacac taaacttatt cccagaaatc
     3481 ggcaagtatc cctttgatat tcttaccaat accattaatg cttttttact tacttatgcc
     3541 atctatcgtt accagttact taatattact atctctattc gcaaaggttt agtttattct
     3601 tttttaacgg caatgttgac gggaagttat ttactgcttg tttaccttct agagacagtt
     3661 attcgtaata atttaggcta cacagctttt ataatcgctc taatcatggc cgcgattatt
     3721 gcgattattt tcgaaccagt aaagaactgg ctacgtgtgc ggattgataa agtgatgttt
     3781 ggcgagattt atgattatcg aaaaacactg aagcagttta gttatcttaa agcctctatc
     3841 ctggatttgg atgaattgac acattcaact ttgggccttt taacgaaagc gctgcaggtt
     3901 aattccctgg cgctgttact gctagatcaa gaaagtaatt tttttgttta tgcaagtgtc
     3961 aatatggatg aaactgtagc caagacagta cgaattgata aaactagtcc cattatccgt
     4021 cgtttagcgc agcataatcc tattttaacc tggcaggaaa ttgagacggc accagaattt
     4081 caagggctct ggcagctgga aaaagacttc ttcattgcca tgcaaatttc tcattttgtg
     4141 ggcattaaac tcgggggaga gttgattggc attctagttt taccccataa attttctcgg
     4201 gatccctata cagatgatga ccgtgagatg ttattaatgc tggctaacga agcagcagta
     4261 gctattcaaa acgcgcagat ctacagtgaa gtaaaacgtc aggcggtgcg cgatgaacta
     4321 accaaacttt ataattaccg tttcttccag gagttcatgg ataaagaaat tgcccgctgt
     4381 cagcgtacta aacaagtctt ttctgtaatt tatttagatc tagatttctt taaagcatat
     4441 aatgatattt ttggtcattt ggcaggtgat gaagcgctgg tcaccgtggg tcgagctatt
     4501 agtgaatcga tacggccctc agatatagct acgcgcatgg gcggggatga attcggcatt
     4561 attttgcccg gtgcgacaag ccacaatgcc ttgggggtag caaagcgcat taaaaatgcg
     4621 gtgcaagctt attttcccgg gactagcaag gatagtgtcc tgaccatcag ccaggggata
     4681 gctacctatc cgcaacatag tcagaataag cagcaattgc tttcctgtgc ggataccgcc
     4741 ttatatgagg cgaaaaatat gggacgaaat cgggtttgcc tttatcaacc acagctggtt
     4801 agaagcgatg gcaccactgc tagtagtaca actgctgaaa tcgaaagaaa ttttctcata
     4861 caacaggtgg aagatgctta tttgtcagtt atttatactt tagcggcagc catcaacgct
     4921 aaagataact atacttatca gcactcggaa gctgtaactt attattcgat agccatggct
     4981 gtaatattag gttttgctga ggagcaaaag aagattctgc gttatgcggc tatgctgcat
     5041 gatatcggga aaataggtat ccccggatat attctaaaca agcctactgc cctgactaga
     5101 gaggagcggg aagtcattga aagccatgtg ctggtagctg agggcatcgt cagtcatacc
     5161 ccttacctac gagaagtggc gccaataatt ttgcatcatc atgaatccta tgacggcacc
     5221 ggctatccgc atggattaat aggtgaagaa ataccaatag aagccagaat cttaactatc
     5281 gctgatgctt accatgctat gatcagtgat cgtccctatc gtcagggaat gctgcctgat
     5341 gtggcactgc agcagatgga taagttatca ggaaggcaat ttgatcctaa actgctgccg
     5401 atctttgcca gcattatcag agaacagcag aagcgcaggg ctgcaggtga agcttatgcc
     5461 tagtgcggcc aggaacatta ttgccatggg cgtggccggg tatctacttc tgcttattta
     5521 tgatttagcc tcattgagaa aactacctta tcgctttctt tttagtttgt tgggttatag
     5581 tatgcaagct tatgcgattg tcagcgcagc cttgttcgag cagcagctgg ccgtaagtaa
     5641 atatctctgg ttgttagcgt ggccactgat gttggctggt ttaagctggt tactttattg
     5701 tcttttcctt tatccgccgt taagaaggac ttatcgcgac aaaaaaggtc ccgccttaac
     5761 aaccgaagga ccctatgctt tgtctagaca tcccggtgta tatggctata ctttttttgt
     5821 tttatcacta gctgtcatca gccgttccca gcttttattg tctgctggct gcctttggag
     5881 tgtgattaat ctaatttatg ttttccttca agatcgtttt atttttatgg ctttactaga
     5941 gggatatgag caatatcgtc gcaatacacc gatgttaatc cccaatttgc agagtattaa
     6001 acgcttttgg gcaacaaagt aatgcatttg aggtgtaatt atgatcactt tgcaagaact
     6061 actaaataaa gatcgtcgca aagatgcttg gctcagacat tgtggtttca tagacttgtc
     6121 gctggtcgag tttcaagagg tgcaggaaag attactactg gagcaaattg agatgcttgc
     6181 tcgaagttat cttggcaaaa aacttttaca cggcaacttc cctcgtaatg gggaagaatt
     6241 tcgcaaatct atccccctga ctacctatca ggattatcat ccctacttta aagataaaca
     6301 ggaagatata ctaccgttaa aaccaagcta ttgggtctgc acttcaggtg ctattgatgc
     6361 ttacgaatat aaatgggccc cttttccccc aggtttagcc caaacccata ttaagaactt
     6421 tcttgcctct gttatctttt ctgtgtgtac taaaaagggg gagttttcgc ttaatgaata
     6481 cgctaaattt ttatacgcca tggccccccc gccttatttg acagggatgg tgccctatgg
     6541 cttagaaaaa gagttcccct ttacctattt gccttcacta gaaacagcgg aaaaaatgag
     6601 ctttgaggaa cgtaacaacg atggtttccg tttaggctta acccagggga ttgatctctt
     6661 ttttggtgtt tctagtgttt tagtgagaat gggagaaaag tttgcgcaaa ctaaaaatga
     6721 tttctcacac caggaaaagt tgaatatacg agctttattg cgcatggcca ggggaatggt
     6781 acgctgcaaa ctacaaaaaa gagaactttt gccgcgtgat atctggtcac taaaaggtat
     6841 tatttgtgca ggaacagata ctaattttta taaagagcgg attgaatatt attgggggaa
     6901 aaagccctta gaaatttatg gcggcaccga aataggtatt gcaggtacgc agacctggga
     6961 ttatgagggc atgaccttat tcccggatgc aaattactgg gagtttattc ccgaagacga
     7021 gtactttcgc tcgaaagcag atctaaatta ccagcctact accgtgctgt tgtcagaatt
     7081 aaaagcagga aaaaggtatg aattggtgat taccagcctg aaaggtggtg cttttgttcg
     7141 ctaccgcgtt ggtgatatga ttcgcgtctt aagcttaaag aattcagtct tgtccattga
     7201 tttaccgcaa atcatctatg aagatcgtat tgatgatgtg attgatctcg ctggctttac
     7261 tagaatctat gagcgtacca tttgggaagc tttaaaagag gcaggcataa cccatagtca
     7321 ttggctggct tgcaaagcat ttacgggagg tcatcccctg gtgaaattat acttggagct
     7381 gcctgcgggc tgcacacagg agttgatgga agataaaata caccaagccc tgtgtaaggt
     7441 taatgctgac tatgatgatt taggtcacat gatggcctat cgtccactgc agcttgcagc
     7501 agcgcctagt gctgcttttg cgaaactaaa agagaaacaa ggacaggcgt cggtcgaccc
     7561 cgctatcttt agaagattta atcctccaga atacttaatt aaggaactct ttgcctagaa
     7621 acaaaacaac ctgacttttt agctcaggtt gttttgtttt tggtaggtta atgatcaaca
     7681 tgaactgctc tatgctaaat aagtgagggt ttttagagaa ctacaaatta ttttactgct
     7741 gtctggaggt attagcctga agttgttgaa taattatcta ttcttacagt tttcatacta
     7801 attctcgtta gccaatatta ttagcaggag acgctaacag actgcttgag taaagttgat
     7861 cttgcttact tgagccgctc aattttgggc agcttggtaa gtcaaagaaa ggggaaacta
     7921 actaaatgac agatattagg gctttggaaa aagagcggga gcaaatcttt attaacttat
     7981 accgcggcaa agtaccgaca cgtgtgccgg taagtaatcc catggcgctt gaggcttgtc
     8041 ttgaatatgc cggagcaaac cccgtcgaag ttttatggga tgtgagtaag acggaagccg
     8101 tttttgatca agtcaatact gatttttatt cggatacgtc agtggggggc acgcgcagat
     8161 atccctcctt ttatcaggtt ttaggtgcca gatcttttgt gatgagttcc accggagcga
     8221 tgcagcatcc taatgtgcat gggatggaag ctgcagaata tgatgatttt attgacaatc
     8281 cctatgacac aatcatggag aaaatcttgc cccgcttata tacagaacta gctacggatg
     8341 ctgcgaccag agctgcggtt ttagcaaaag cgatgaacat ccgcagtagt gaatttgcgg
     8401 tcttaggtag aattggtgtc aaaatgagag aaaaatatgg ttttgcagct atccccggta
     8461 atgccacaac cgcgccattt gattttatag ctgatttttt ccgtagtttt acagggatct
     8521 gcagtgacat tcgacgttac ccagaaaagg tcattgccgc ttgtgaagct atgaccccca
     8581 ttaccattaa aaaagggagg attccctttc cgtccaagat cggggtaaca ggtatcccgc
     8641 tgcatatggc cccttttatg cgcaccaaag attttgagaa attttattgg ccttctttga
     8701 aaaaggtagt ggaaactttt catgctatgg ggatcaatgc ttcccttttt gtagaacacg
     8761 actttatgcg ttttttagac tatttatctg aactgccgga aaacacgatc ctgcgctttg
     8821 agtatggtga tcccaagata gtaaaagaaa aattaggcag taagcagatc atttccggtt
     8881 tttatccggt gatgttacta cataccggca ccaaacagca atgcttggat aaagctaaag
     8941 agctcatgga tattttagcc cctggtggtc ggttctggtt taataccgat aaaggcccca
     9001 tggacatgca tggtaatatc gccgaaaatt tacgggctgt actggagttt gtttatgact
     9061 atggtaaata tgaacatcct gacgagccct acaatcttgc ccatgagccg cagccgcagg
     9121 ccgataaagt aattaacgaa atcaatgcta acctgaactc caagtattac tatacctggc
     9181 aggactacca aacccgttat cccgaactgg cacaacgtac agagcaaatc attgcacgtc
     9241 aactgcaaaa aaacgaagat gctttctttg atttcattat caacctctgt tcttgatagc
     9301 aaatgggcag ctaacagcta gttaacaaga aaagtacgcc ttaagaaatc agcgtacttt
     9361 tcttgcagtt ttaggaagat gttgatataa tggcaaaaat aattattcat aaaggagtag
     9421 gggctaatgg cggaaaattt gaatcccttt gaaattgtac agcagcagat tagcgaagcg
     9481 ggtaagtatt taaatttaga cccagcgatt gaagccgtac ttaagcagcc gaagcgtgtg
     9541 ctgacagtta cctttccagt aaagatggat gatggcacaa ctagagtatt tacaggaatt
     9601 cggtctcaac acaacgatgc tattggacca tgtaaaggcg gcattcgttt tcatccggat
     9661 gtgacggtgg atgaagttaa ggctctttcg atgtggatga ctttcaagtg tggtgtagtc
     9721 ggccttcctt atggtggtag taaaggcggc gtaatttgta atcctaaaga gttgtcacag
     9781 ggcgaaatcg agaggattag ccgtggttac attgagggaa tagctgctat tattggccca
     9841 gacaaagata ttccagcccc agatgtttat accaatgccc agattatggc ttggatgatg
     9901 gacgaattca gtcgtttgcg gggtactaat caatttggtg tagtgacagg gaagccaatt
     9961 atcgttggtg gctcgttagg ccgcgataaa gcaactgcac agggttgtgt gtttgtagtt
    10021 atcaaggctg tagaaaaact tggtattgat ttaaacggag cgactgttgc cattcaaggg
    10081 tacggtaatg ccggttcgca agcggcattt ctgttacata aactaggctg caaaatagtg
    10141 gcagtgagtg actcccgtgg tggtatttat aagccagaag gactggactt ggctgctctt
    10201 tatcagcata aaactgatac ccgttcctgc attaatttcc cgggtagtca gtgcgtatcc
    10261 aacgctgaat tattggagct gccggttgac attctcattc cggcggcctt ggaaaatcag
    10321 attaccgcca gtaatgcccc tcgggttaaa gcaagaatcg tagctgaagc agcgaacgga
    10381 cctactaccc ctgagggcag taaaattctt tatgagcatg gtaccacagt tattcctgat
    10441 attctggcta atgctggtgg tgttacggtt tcatattttg aatgggtgca gaatttaatg
    10501 aattattatt ggtcagaaga agaagttttt gaaaaattgc aggtgaacat ggataatgct
    10561 tttgaccagg tatatgcaat tagtcagcag catcaagtag atttacgcac agccgccttt
    10621 attgtttcca ttaaaagagt agcggaagca attaaaatta gagggattgt gtaagctttg
    10681 tagtaaaaca agaccatggc ctggaaattt gtgccatggt cttgtttttt tagttggtct
    10741 gcctgaggct gctttctttc ctagccgcta aaggatataa tattatagta tggttgggaa
    10801 taaaatagtt tgtgaggtaa gtcaggtgac agcctatgtt tatatagtaa aatgtgcgga
    10861 taataccttg tatactggct ggacaacgtc gttggtgcgc cgtgtcaggg tgcacaatca
    10921 aggcaaagcg gctaaatata cgcgggctag gcgtcccgta gagctggttt actgggaaag
    10981 gctagattca aaagttgctg ctttgcagcg ggagtgggaa attaaacaac tgaccagagc
    11041 ggaaaaatta gctttatgta atatgaagct caagtattta ttaagggaac gcggtgatga
    11101 gacaacatga aacagatcat taaattggtg ctcatcgtca ttggatctta aagaagaaga
    11161 tattttaacg ggagaggaac cagtgaatga agcagaataa aaaattggtg tgggttttag
    11221 tatttgtttt aacagcgctg gagcagggta taaaaattat tataaatcgc tattatctac
    11281 aggtaaaggt ccccctggtg ccgccatgga tttattttgc tcccttattt aatcgagatt
    11341 actcttggat taattcgctg ctgcagctgg gtgtgggtaa agtagtacat ataatgattg
    11401 tagtagcagc tataatactt atctactttt tttaccgtta cgttgccgat cgctataaag
    11461 ttaccgcctt agtcggcata tcttttgcac ttttttttgc gggcgcactt tgttctttgc
    11521 tagacaaagt tttttgggac gggagtttag actatctact tgtgacgggc ttctttacct
    11581 ttgatttaaa agacgcttat cttaatgtgg ctgtagcctt gattgtgttg atgttggcta
    11641 ttgatcatca gggaatgcgc accgcggaat tctagttttg ttagatcaag gacggctgcc
    11701 tcttgaggga gttgaagggg cggtgatgat gctggttagt gataagacat ccaactcgcc
    11761 caacgtgggc gagtaaagaa agacgccgat ttacaatcga ggcgtctttc ttgcattatt
    11821 ttggctgtgt tgcttatatt agtaacgact attaagtaat gatgaggtga atagatccta
    11881 atgactaaag taacagcttt tgaacaacct ccccatccct attggctggc ctctagtccc
    11941 caaactaact atcctcgttt agaggaagat gtcacagtgg atgcagcaat tatcggcggc
    12001 ggtattacag ggattacttg tgcctactta ttaaaaaagg caggtcttac cgtggcagtt
    12061 gtggaagcag atcatctggc tcaaggagct accggtcata cgacggctaa aattagctcg
    12121 caacatggcc taatttatca aaaaatcaag cagcaaatgg gacaagctgc agcacagcaa
    12181 tatgctgagg ccaatgaaac agctatccgt cagtttgaga agattatcaa agaggaagaa
    12241 atagcgtgcg atttttcacc acaagctgcc tatgtttaca cagaacggga cggttataga
    12301 gaaaaaatag aagcagaggc cgagctggcg agcaaactag gcattgaggc cacagtagca
    12361 gagacaattg ctttaccttt ccccgtaaag gcagcattac gttttgctaa acaagcacaa
    12421 ttccatccca gaaaattttt gttaccgtta gcagagagcg tgcccggcaa tggcagttat
    12481 atttttgaac aaagtaggat tgtaggtata gacaatgacc ataactacct gttaaaaaca
    12541 gctgatggta ataggattca tgctgaaaag gtgatcattg ctacgcacta cccctgctac
    12601 aataaatctg ggttctattt taccagaatc tatcaagggc gctcttatgt cgtggcagtc
    12661 aaggctcagc agccataccc agggggtatg tatatcacgg cggaagaacc gggccgctcc
    12721 tttcgctctt tgcccacggc tgagggtgag ctaattatgg tgggcggaga gcaccataag
    12781 gcgggccaag gggctgatac cagagaacac taccaagctt tagcgcaatc tgctggggag
    12841 atttttccac agtcagctat ttgctgccgt tggtcaacac aggattgtat tactttggat
    12901 ggcctgccct atatcggtca gtttttgccc agcacaccca atttgtatct agcaactggt
    12961 tatcagcgct ggggaatgac taatagtatg gtcgccgcga tgctcctgcg agatttaatt
    13021 accaagggtg aaagtccttg gcaggatgtt tataaccctt cacggctaac gcttgctgct
    13081 tcagcgaaac cctttgttac tgtcaacctc aatgtggcta atcaactaat taagggcaaa
    13141 ctggctactg cgccaacgga agttgagctt aagccgggcg aagccaaagt gatgatggtt
    13201 aatgatgata aggtgggcgc ttaccgtgat cagcagggta ctctacacct tgtggataca
    13261 agctgcacac atttaggttg tgaactgcag tggaatgccg ctgaattatc ctgggattgc
    13321 ccttgtcacg gctctcgctt tacttataag ggagaaatta ttgaaggtcc ggcaataaag
    13381 gcgcttaagg caggccaaga gcatagaata aaccgtaaag aaaaatcctt gacctaggtc
    13441 aaggattttg ttcattgtag gccgtatatg ttttgttcac taatcgggcg tcctgtatct
    13501 tctaattcag caagggggac agttattttg cggttggtgc ctataattga aatatctgct
    13561 gttgaaccct ggacattttc aatccatact ggaacatcct gatactggac ttcaattttt
    13621 tcactagcct tgataatttg ttctgctcgt tgattgtcca cgagatccct ccttttgttt
    13681 tagtatgtga caaaggaaga gtggactatg cagcttttgt ttttttctgt ccgtcatgct
    13741 cttgctaagc agcagaatta attgtataat atatttagaa tattgcgggt atggcttagt
    13801 tcattatctg ccggcctaaa aagaagacct aactgctgaa aagcaggcgt tattcttgtg
    13861 aaagctcttg taaattgaag gtaacaacta tataatatat tcagaatatt aagaagaaag
    13921 gccctctttc tcgatcgcca tagtctagca cttaaaaggt ggttatgtct atgaaagtgt
    13981 aaatcatcat catgtacaag ccttatttgg ttgtgccttt atctatgcag cgagaagaac
    14041 tttagttcag cagtaatgct gcaagcagct gcgattaggc agcgctaacc caattttctt
    14101 tttacattaa gtatctactg aagaatggta tcaataagaa caagggggaa tttaaatgtc
    14161 aacaaaagaa ccattaagta ataaggttat taacttattt agcattggtt tgtctccgtt
    14221 tatgctcttt atgggccctg tcatggctta ctatccttat tttctgactg atattgccaa
    14281 aatatccgca actacagttg gcgtaatact tgtagtcacc cggatcgttg atgcttttgc
    14341 gataatcttt gtcggtggta ccattgaaag atcaaactta aagtggggca aatatgtatc
    14401 ctggatttac attggtgcac ctcttactct ggtcttcttt gccctcatgt gggtaaatcc
    14461 tggtggcatg ggtatggcag ctaaagctat ttacttcacg gtgatatata ttgtgggtca
    14521 tattttcatg aactttgcct ggagtggcgc ctcgagcttg attccctcga tgacggctaa
    14581 tcccatggat cgtgttaaac tttcagccag acgtacacaa tttcaaacag tgacgatgct
    14641 aatctatagc gctagtcata tgcccatcat tatgtacttt ggtcgaaata acgaaggtac
    14701 aggttatctt acctttatgc tgattgcctt agtaatacag ttagtaggct acctgctctc
    14761 ggccaaaata gctaaacctt atgatactac gacagcatct ggtgctgctg taccttctat
    14821 tggtgatatg ctcaagggga ttgtcactaa taaaccacta cttattttaa tgctttatgg
    14881 gctctttaat ttctccgtgc tatctttagc taatggttta aatatttatg tctttaaata
    14941 tgtacacaat aatgtgcttt taatttctct tttcctcacg gttaactctt tagttaattt
    15001 agcttcttcc tttattggcc aatttattgg tcaaagagtt agcagtaaac gtacagtaat
    15061 gatgattggt actgcggttg ccatgtttag tttccttggt gcctttttct tcgccagaaa
    15121 caccatcgtc tttatgatct tccgctggat cagtgctctg tcaggtatgg gtatggtcgt
    15181 taacttcgca gtttatgctg atgctgctga ctatgctgaa tggaaaactg gcaaaagcgc
    15241 taaagcttta attatgtccg taagttcctt gcctgcgaaa acttcaggtg cgctagttgg
    15301 cgctgttacc ggtttcggtt tggccgccat cggttatacg gcagatgctg cagctactcc
    15361 tgaactagta cagggactta tttacctttc gacgttactg ccggttgctt gtgccttcat
    15421 tgccctgata gccttgtctt tctataagct agacaatgcc acaatgacaa aaattaatga
    15481 tgaattaact gtgagaaggg cagcagctgc aaagcaatag tttatggtct aaacgtaggg
    15541 tgtcggttta accggcaccc tattgttttt gccggcagta aaatagaatt taacgattaa
    15601 cgaagctgtt tagatagcca ggaaaaagcg taagcttttt ttgcagggca gcatgactta
    15661 tggttaagcc tgcaggataa ttcacagctg agggagaaaa atagacttga ggtggtagac
    15721 aaatgtatcg ttctggcttt atagctttag ttggccggcc caatgtgggt aaatccactt
    15781 taatgaatgc cctagtgggc gagaaaatgg ccattatctc agataaaccg cagacaacac
    15841 gcaatcaaat tcgcggcatt cttacgacag aagattatca aatgattttt ctcgacacgc
    15901 ctgggattca caaaccacat cataaattag gcgaaaaaat ggtgcaaatc tctttgcgta
    15961 ctttgcgtga agttgatttg atactctttt taacagatgc agctgctggt cccggtaggg
    16021 gtgatgagtt tgtgatacaa aaaatgcaag cagtggacac gcccgtgatc ttactggtga
    16081 acaaagcgga tcgcagcggc ccggaagagg ctcaacattt aatagagcat tatcgcccca
    16141 tgtatccttt tgtcggcgct ttagccattt cagcccttga gggaacaaat ttagctggtt
    16201 taacggagct gatgttaaag catatggcag agggtcctca gtattatcct gaagacatga
    16261 ttactgatca accagagcgc ttaattgtct ctgagttaat tagagaaaaa gttttgcagt
    16321 ttacccggga agaagtgcct catgctgtgg cggtggagat ttcctccatg tctgcacgta
    16381 gtgatcgtga tttggtggat attcaagcta atatttatgt ggagcgtgat tcgcaaaaag
    16441 gcattgttat tggaaagggc ggctccatgc tgaaggaaat cggcaaatta gcacgcctgg
    16501 atattgaagc atttttaggc agcgccgtta atttacagct atgggtgaaa gtaaaaaatg
    16561 attggcgtaa taaagaagga acttggcagg cattgggttt agaactggaa taggggaata
    16621 gttgatgaca gaattaagat gtcaggctgt agtgctgcgt gtgcgcagct ttaaggaagc
    16681 cgataagttg gtgacgctgc tgacgcgtga tgccggtctc attaccgcgg tagcacgagg
    16741 aagccgcaaa acaaaaagta aattttccgc cttggtggag cccttaacac tagggcattt
    16801 tcttttgtat caaggacgaa ctctttatac ctttattcaa ggtgaaataa ttaaaacata
    16861 ccgcagcctc cagggggact tggcacgttt cgcttatgcc cagtattttt gtgagctgtg
    16921 tgagcgttct ttactggaag gaatgccttc tgaagcgatc tatatgttgt tggtaacagc
    16981 attggaagct ctggagcagg accatgaccc ggcacgtgta gctcgttgct ttgagctaag
    17041 tctcttggta gaattgggca tcagtccgat tttagatggc tgtagtcatt gtggcagtac
    17101 aagcggccct tatcattttg atcccacaga gggcgtttta agctgtcggg cctgtccgca
    17161 atctgtagag agtttccccg ttagcgcggc gactattgcg attacgaagc gtttgctgga
    17221 aatgggattt cataagcttt ccgtctgtgt gattccgccg acagaaagta aagagattca
    17281 ccgtctggct gcggatttac ttcagtattc ccttggtatc ggtaaactaa aagcgcttga
    17341 ttttttacaa aaaaccggct tatggtaatg acggctcaat aaaattaatt gtcaaatatt
    17401 tttactgccc ttgtgacaaa ctagcaatat taatgccaaa ggagggcaga aaaaatggaa
    17461 ataaccctac agaaaattga gcaggttcgc gaacgcagcg gcttaaacta cacggaagca
    17521 cgggaattac tagaagaagc caagggtgat gtcgtccaag cactggtcat tttagaaaat
    17581 gaggaaggtg atttgggcat ggatatggag atggatgaga tgaaaggtat ggttagtcat
    17641 agtgttgttg atccagtgaa acgtgttttt cataaaagta atcgcacgcg catccgtgtc
    17701 aaaaatgagg atggcacttt actggaaatc ccagccacat tggggattgc cggtgctctt
    17761 ttagcccctc gggccaccgc aattagtacg atggctttgt taatggcgca gtactcttta
    17821 gaagtagatg tgccggagta cgtagcgctt gacgagcctg agtggactta aggtaaagag
    17881 gtattgtctc ttggcgtaaa aagcttgacg tcttgtctgg tttttgttac agtattgtca
    17941 aatgaaaaca ccgtgagaag aggagtacga tgcggaccag tcagcgaacc ggggaatggt
    18001 gcgagcccag tctgggataa gcatgggaag gcgcttttta acagcggtgc aaaatgcgag
    18061 tgggcatcta agatgccaag tagggtggaa ccgcgggaac ttcccgtccc tatgggacgg
    18121 agttcctttt ttttatcctg acaggaaatt tacatagctt aatttagcaa gcgcgctaag
    18181 aaagccagta taaaagctgc agtttgcaac tgaaagcgca agtttattta attaaattta
    18241 ggagggtatg gagaatggat tttcagacta tgattctgaa actacaaaat tattgggcca
    18301 gacaaaactg tgttttggcg cagccttatg atgtagaaaa aggtgctggg acgatgaacc
    18361 ctactacctt tttgcgtgcc ttaggccctg agccttgggc tgtcgcctat gtggaaccat
    18421 cgcggcgtcc gacagatgga cggtatggcg agaatcccaa ccgtctgtat cagcatcatc
    18481 agtttcaggt gattatgaag ccagcaccag atgagattca agctttgtat ttagacagtc
    18541 tgcgagaact aggcattgtt cctgaagagc atgatattcg gtttgtggaa gataattggg
    18601 aatcccctac tttgggggcc tggggtttag gttgggaagt ttggcttgat ggtatggaaa
    18661 tcactcagtt tacttatttt cagcaagttg gcagtatcga tgtagatgca gtgtcctcag
    18721 aaattactta cggtttagaa cgtattgcca tgtatctgca gaataaagaa aatatctatg
    18781 atttagcctg ggttgacggt gtgattacat atggcgagat ttttcaccgg gcagaagtag
    18841 aacattcttg ctacagtttt gaagaagctg atatagaaat gttatttacc ttgtttgcga
    18901 tgtatgaaaa agaaggccag cgcttactga aaaaagggct gattttacct ggctatgatc
    18961 aagttttaaa gtgttcacat acttttaatg ttctagatgc tcgtggtgcc attagtgtgg
    19021 cggaacggac agcttatatt ggtcgtgtga ggcatctggc tagattggca gcacaaggtt
    19081 atttacagca gcgagaagca ttaggttacc ccctcttgcg ggcggaaagg agtgctaaag
    19141 gtgcgtaagg agagttttct gttagaaata ggtacagagg aaattccggc acgttttatt
    19201 agtcctgctt tagcgcagtt agcggaggca gcggcggcgg aaatgaaaca gcagcgtcta
    19261 gcatatgaag atattaaagt ttatggcaca ccgcgtcgtt tagcgctttt agtctcaaaa
    19321 gtggcagaaa aacagcaaac attagaggag aagaaaaaag gtcctgcagt aaaggctgct
    19381 tatgatgccc acggtaagcc tacgccagca gcacaaggct tcgcacggtc ccaaggagtg
    19441 gccgtagagg agctgttcac tgcagacgtg tctggagtac cttatgtttt tgctgtgcgc
    19501 agggaggctg ctcatgacgt acagaccatt ttacctcaat tatgtgcaag gttaattcgc
    19561 agcttaaatt ttcctaaacc aatgttctgg cacagtaaag atgttcgttt tgcccgtccc
    19621 attcgctggc tgacagcact ctggggttct acccctatcc cctttagctt tgcgggttta
    19681 acggcggcaa atgtgaccta cggccaccgt ttcctagcag cagaagctgt taagctaaaa
    19741 gaggcagcag actatccaca ggtgatgcag caaaattttg ttatggctga accgcaagag
    19801 agaaaaacaa aaatagcagc tttagtaaaa gaagctgcca gagcacttgg tggtcattcg
    19861 gttcttcatg aagatctctt agaagaagta gtaaacctcg tcgaatatcc gcgtgtgatc
    19921 actggtgaat tcgccagtgc atatttagaa gtacctcagg aagtgttaat cacggcgatg
    19981 cgcacgcatc aacgttattt ccctgtattt ggtgaagata acaagctttt gcctaacttt
    20041 attaccatca gtaatggtac cagtgaagaa tatgccaaga atgttcgtgc cggtaacgaa
    20101 cgtgttttgc gtgcccgttt agcagatgcg cgcttctttt ttgaggagga ccgcaaacaa
    20161 ccactagcag cttatgtgga caagctagat aagatcgtct ttatggaagc attgggaacc
    20221 atgaggcaaa aaacagcaag aaccattaag ttggtacaaa gactagcaga ggaattaaag
    20281 gtagacgcct ctgtgcgaga agctgccgag cgcgccactt tcttggctaa agcagattta
    20341 atgacgcaaa tggtgtctga gtttcctgaa ttacaaggaa ctatgggcat gcattatgcc
    20401 cgcttgtccg gtgaaagtgc agcagtagca acagctattg atgaacatta tgcgcctcgt
    20461 tttgcaggcg acaccccacc agccactttg cctggagcgc ttgttgcgct agcagataaa
    20521 atggatactt tagcggcttg ttttgctctt ggcttaattc ctaccggctc acaagatccc
    20581 tacgctttac gacgtagtgc tgctggggta gtggttactt tagctgatca tagcttacca
    20641 ataaccattg agcgcttatc tgaactggca ctgagcgagc tgcaggcaaa cttaacgcag
    20701 cctgcagccg aggtcacagc acagctggca gattttatgc tgcagcgtgt acgttacacg
    20761 tttgtcgagc agggattgcg ctatgatgtc attgaagctg ttttagcagg cgctgtgcat
    20821 aacttgcccg ctttatggca aagagcgcaa gtgctgcagg ataaactgaa ctcgcctgaa
    20881 ttaacacaga ttctcattcc ctttacacgc gtagcaaatc tttcccgtag tgagcacagc
    20941 gaggtcgtgc agccaaagct cttgacggcg gttgcggaga aggaacttta tcaagcttta
    21001 ctcactgctg aaaagagcgt acaagctgcg gcaggacgta gtgactttac ggcggtcttt
    21061 acagcacttg cgccacttta tcagccgata gaaaactttt ttgccgatgt gatggtgatg
    21121 gcagaagatg tgaccgtacg gcagaatcgc ttggccctac ttgcacagat aaaaaaacta
    21181 tttttaacgc tgggcgatat ttccacgatt gtgccggaaa aaaaataaat ttgtcagttt
    21241 gtcaggcagg aaactgaata tgtatatagt atatactata tgaagttttt ttgagatata
    21301 gtgtatacta tttgacgtgg ggagggtccg ataatcgaac taaataatag gcaaaaggaa
    21361 attttggcga ttgtaaagca agatgggccc atttcgggag aagaaattgc cggtcgcctc
    21421 aatctgtctc gggctgcttt gcggccgcat cttgctgtcc tcacgacttc aggtttacta
    21481 gacgcacgcc cacgtgtagg ctatttttat acgggcaatg ttttaactgg aatctcggaa
    21541 atacttacac gttttaccgt aggtgatatt aaaagtcgtc ctattgtgat tcgggaagaa
    21601 acctcagtgt acgacgctat tgtgaccctc tttttagatg atgttggcac cctatttgta
    21661 gtcgatgaaa aaggcaattt acaaggtgtg gtatcacgca aggattttct caagacggca
    21721 ttgggtggtg tagatctgca taaaatgccg gtaggtgtaa ttatgacgcg catgccgcaa
    21781 gtccatactg tgacagaaga agaaacagtg ctggaggtag ttaagcgtat cagtgagcac
    21841 gaggtagatt ctttacctgt agtgcgcatc caagactcac aaacatataa agttgtgggg
    21901 cgagttacaa aaacaaatat agcccgtctg ctgctggaat tgagcgaagg ccgctaaaga
    21961 ggtgataaaa cgcttggcag aaataaaacg taaaccaatc gtctatgtgg tttctgattc
    22021 tattggtgag acagcagaat ttgtcgtgca agctgtggcg agccaattta attcaggaca
    22081 tgtggaaatt cgtcgtattc cctttgtcac agatctggaa acgatcgttg atgtcgtaga
    22141 agaagcttca ctgggtaatg cgatgattgc ctataccttg gttttaccat ctttaagaga
    22201 ggctattcgc gaagaaagtg atctccatgg tttaattgcg gtggatgtga tggggccgat
    22261 gatggatgct ttcaggcgcc taatgcagct agatcctcac caagaaccag gcttaatccg
    22321 tcgtctcgat gaagagtatt ttcgccgcgt agcggccatt gaatttgctg taaaatatga
    22381 tgatggcaag gatccacgag ggatgttgtt agctgatgtc gttttagtag gtgtatcccg
    22441 tacctccaaa acacctttat cgatgtatct tgctaacaaa cgtcttaaag tggccaattt
    22501 gcctttggta ccggaagtgg aaccccctga agaattattc tgggtgccgg cctctaaagt
    22561 gataggtctc acgattaatc cccgtcaact taatgaaatt cgtcaagaaa ggcttaaagc
    22621 gctagggtta catgcccaag ccaactacgc tagtatggag cgcattctgg aagaattaga
    22681 ctacgcagaa aaaattatgc gcaaaataag gtgccctatt tttgatgttt ccaacaaggc
    22741 tgtcgaggaa gtggccaaca aaattctaca attagttagg gaggaagctc gggatggaga
    22801 ataagcatgt ctatttattc aaagaaggaa atgctcaaat gcgggagcta ttgggtggca
    22861 agggggcaaa cttggccgag atgaccaaga ttggccttcc cgtaccacaa ggttttacag
    22921 tttcgacaaa tgcctgcctg gaatatttaa aacgtggcga gcaaaccatc tgggatgagc
    22981 tgcgggaaga agtaatggca gcgctgcaaa ctttagaagt aatttccggt aaaaaatttg
    23041 gagacaaaga aaaccccctt ttagtttcag tgcgttctgg cgcggtgatt tcaatgccgg
    23101 gcatgatgga tacagtactt aatctaggct tgaacgatga atcagtttta gggttagctg
    23161 tggcagctaa tgatgagcgt tttgcttacg attgctaccg tcgctttatt caaatgtacg
    23221 ctgacgtggt gcaaaacatt gatcattaca atttcgagca gaaactagaa ctgaaaaagc
    23281 agtctttggg tgtcaagttt gatactgaac tggatgcagc tgctttaaaa gaacttgtcg
    23341 aagaatataa agcaatcgtt ttgaaagaaa cgaaaaaaca cttcccgcaa gaaccagttg
    23401 agcagttaat tttggctatg caagctgttt ttgcttcctg gaataatcaa cgtgctatta
    23461 tctatcggca gattcataaa atatctgaca gcttaggtac tgccgttaac gtgcaaatga
    23521 tggtttttgg taaccttggt gatgatagcg gcaccggggt ggcttttaca cgtaaccctg
    23581 ctgatggcac taataaacta tatggtgagt ttttggttaa tgctcaaggc gaagacgtcg
    23641 tggccggtat ccgcacacca ctggccatta gtaatatggc agaatacttg cctgaagctt
    23701 atcaggaatt tactaaagtc tgcgacttac tggaaaagca ctaccgtgat gtacaggaca
    23761 ttgaatttac ggtagaacgc ggcaaactgt tcttgctgca aaccagatct ggtaaaagga
    23821 cagctgcggc agctatcaag attgctgtta atatggcgga agaaggttta atcactaaag
    23881 atgaagccct gcttcgtgtt gaccctgctc agcttaatca actattgcat cgccgtattg
    23941 accaagatgc taaaccggaa ccaattgcta aaggcttacc ggcttcacct ggtgctgcct
    24001 gtggcgagat cgtctttaca gctgatgagg ccgaccgttt agccggtgag ggcgttaaag
    24061 taatcttgct gcgtccggaa actacccctg atgatatcca cggtattgtg gctgcacaag
    24121 gcgtcttaac cagccgtggc ggcatgacca gtcatgctgc ggtagtagcg cgcggtatgg
    24181 gcaaatgctg tgtatccggt tgtgaagaaa taaaaattga tttattagct aaaaccatga
    24241 ctgctggcga gcatgtcttc aaagaacggg atctcatctc ccttgatggt tctaatggtg
    24301 aagtttatgc gggcacggtt aaattaatcg ccccagaatt aactgatgaa ttcaatacgc
    24361 ttttagcttg gaccgatgac gttaaagatt tagggatacg tgccaacgct gacaccccag
    24421 aagatgcgcg taaagcgcgt gaatttggcg ccacaggaat tggtctttgc cgtactgaac
    24481 atatgtttat ggcgcaagat cgtctgcctg tcgtgcaaaa gatgattttg gctaaaaatg
    24541 aagcagaacg tgaaaacgct cttgctgaac ttttacccat gcagcaaggc gatttcgaag
    24601 gtatcttccg ggccatggac gggctgcccg tcaccattcg cttattagat cctccactgc
    24661 acgagttcct gcctaaccaa gaagacctgt tagttgatgt aaccttgtta aaagagcgcg
    24721 gcaatgatcc gcagcagctg cgtgaaaaag aagaactctt aaagcaagta cgtggcctgc
    24781 atgagattaa tccgatgtta ggtcatcgtg gctgccgtct cggcatagtt acaccggcta
    24841 tctaccggat gcaggcattg gcaattttcc gtgctgttgt tgctttaatc gcagaaggca
    24901 agaccattat tccagaagta atgattcctt tagttggtct ggaaacagaa ttagagttaa
    24961 tgcgtgcttt agtagtgcag gctgctgatg atgtgatggc cgaagcggga attaactttg
    25021 actataaagt ggggacaatg attgagctgc ctcgtgcctg catgactgct gataaaattg
    25081 ccgcacatgc tgatttcttc tccttcggga caaatgactt aacacaaact acttttggct
    25141 attctcgtga cgatgcggaa ggcaaattcc tcctagacta tctgcagcaa aaagtactgc
    25201 cggaaaaccc cttcgcagaa cttgaccgcg agggtgtcgg ggctctggta aaaatagctg
    25261 tagaaaaagg tcgtgccgtc aagccaggcc ttaaacttgg tatttgcggt gagcatggcg
    25321 gtgacccagc ttcaattgag ttctgtcact tagtagggct aaactatgtt tcctgctcac
    25381 cctttagagt tcccatcgcc cgccttgccg ccgcacaggc aaaactaaaa aataagtaat
    25441 aagtaacaac aagagctgtg cccactaacc ggcacagctc cttaatttaa ggaaaaaaag
    25501 catagtaagc gagagataaa aagaggtgca atccaataaa gaaagtagta ttatttagtc
    25561 aacaacgcta tggatagatc gtttacaagg tagaggaggt ttgtatattt tgattgcggt
    25621 agtttatttt tctcgcgata ataatacaaa agccggggcg gaatttctgg cggcctcgct
    25681 tgaagggacg ttggtaagtt tagtggagga aaagcgaagt agttttatgg gcggggcctg
    25741 gaaggcattg agaggaaaac cagtaaaatt aaaaggtgag ccatggcaag aagtgcaagc
    25801 ggtggaaaac atctatttga tgacgcctat ttgggccaat aatggtgctc cggcaatcaa
    25861 tgagtttttg ctaaagaccg acttttctgg taaaaaagta acgattatta cttttcaggc
    25921 ggatgctagc tttaaaggca gcgaactagc acatcagacg atagctgcat tgattgaaaa
    25981 aaagggcggc atggtgctcg cttgtcatgc ttgtcatggg gccaggcctg gcgcctttgc
    26041 gggtaaagaa tatatagaaa gacaagtagc tcaagtaacc ctacagcagt gatagtaacg
    26101 cagataaaag cctcttaccc atgggtaaga ggcttttgtg ataacaattt aagctcgttt
    26161 aattaagcca cggataaaat tagcgatact gcggaagaag cggacaaaga tattggctcg
    26221 cggtaccttg gtggcggtga ccaatggctt gctggcaata gtctcgccgt ctagctgtac
    26281 cagcagcgtt gctgcctgac tgccctgctc tactggtgct tgcaatgttt tttcttcagc
    26341 aacaacaagc tttaaatcac ctgcacgatt tggcggtgcc agcacgatta aatcttcagt
    26401 gactgttaaa gctactgttt gctttttacc atctttaact ggagtctcgc ctagagcatc
    26461 atccttggct gcaggctgcg tgaaaacaaa attacgaaaa ccgtagttca gcaactcttg
    26521 actggcaatt aaacgttctt gatcactgcc tgtattcagg gtgacagtga ttaagcgcat
    26581 gccattttgc tctgctgtcc cggctaaact gtagcctgat tcaggtgtcc agccagtttt
    26641 taaaccatct gccccttgaa agcgacctaa aagcgggttg cggttttgtt gtttaatatt
    26701 attgtaggtg tattcacgct gcgactcgaa ttctaagatc ttggggtgtt tgacaattag
    26761 ctcgcgagcc aaaatggcaa tatcacgggc actcatataa tggttctctg cgggcagacc
    26821 ggtagcgttt tgaaactggg tattagtcat gccaagttcc tgggctcgct gattcatccg
    26881 ctgcacaaaa gcttcctcac taccagctaa tagttcagcc atggcgatac aaccatcatt
    26941 tgccgagaca atggacatgc cgctaattaa ttcttctaca gaaacttcag tatctacctg
    27001 cagaaacatg gaggagcctt ctttttgcca ggctcgttca gagacaatgg ttgtggtatc
    27061 ccactggatc tcgccgcttt ctattgcttc aaaggataac aagagtgtca tcactttagt
    27121 aatactggcg ggtggacgag gttcgtcagc atttttcaaa tacagcaatt gtccggtaga
    27181 agcttccatt agggcagcca caccggcaac agtattaaaa tcttgtgcag ctgcaggtac
    27241 agagagtagg aacagcagta tagcaagtag taatgagagc acacgttttt ttgtcattta
    27301 atacgcctcc tggagcagaa acttttacat acgtatatct tatacgaaaa catagggaag
    27361 aatccagagc taaagcaagg aaagtaaaaa atttgtccgc attacctaaa aaaaatgcta
    27421 aattgtgaag cttgtcttta tcatgtataa tattaataat taattattca gcagggaaag
    27481 tagggagcgg gcttgagtga gggtttaatt ctagttgttg atgatgagga gcatattcgt
    27541 gaactagtcc gtctttattt agaaaaggcc ggttttgccg cagccctggc tgcggacggt
    27601 gaggaagcga tacaaaaagt aaaaagtctt gtaccacaat taatcattct tgatattatg
    27661 ctgcccaaaa tggatggctg ggatgtctgc cgtgaggtgc gaaaaattgc cgagacaccc
    27721 atcatcatgc taacggctaa aggtgaggaa tttgacaaag tgttagggtt agaactggga
    27781 gcagacgact atctgaccaa gccattttca ccacgggaac tggtggccag agtaaaagct
    27841 attttacgcc gggttgttgt agatgatatc gccgcaaatg aagatataat ctctttacca
    27901 ggtctgaata ttaaccaaag ctcccatgaa gttcatgtgc gggagcgtga agtgaacctg
    27961 actcctaaag agtttgattt gctctggtat ttagcaaaga acaccggcaa agtttttacc
    28021 cgggaacaac tgttgactgc cgtgtggggc tatgattact acggtgactt gcgtactgta
    28081 gatacgcata tcaaacgctt acgtgaaaag gtggagcggg aaggaacagg aacaaacgct
    28141 tatcagatta aaactatctg gggcgttggc tataaatttg aggtgcagga cgcatgaagt
    28201 taaagatttt ctcaaagctg ctgacgacat acttggtgat cattctgatc accttatttg
    28261 ttgtgggctt agcactaacc cagcttttgc aaaactattt ctatcatgcc cgtgagggtg
    28321 aattacaggc caaagggcaa gaactggcag acatttacgc agcgcaaatg ctaggttttc
    28381 aggatccacg tgttaccagc gacttactat ttgcgctgga tcgttttctt gatgctcggg
    28441 tccagctggt ggacagatcg atgttgggat tggcgacttg tcctggtttt gaagacctgg
    28501 gcctgccatt aacgacggca gaacttgatc tgctcttgaa cggccatact attaccaaac
    28561 gcggctatca ttcacagtca aagctgcaaa tggcaacagt ggtggtgccc gtggcagctc
    28621 ggcaagaagt tttgggcgcc atcttcctgc acgcacccct aaccgggatt acacagacgg
    28681 tgaagcaagt tcaggaactt attttttatg cagctatttt agctaatatt ttggccatga
    28741 tcgtgggcta ctatatgtct aaatctattt ctcatcctct gcagcaaatg acccgtgcgg
    28801 cgctggaagt agccggtggt aattatcagc agcaggtaga agtaccctca gccgatgagg
    28861 tgggacagct ggctcaatct tttaaccaca tgtccgctac actttaccaa acggtggaag
    28921 ctctctctca ggaaaaaaac aaactagaaa atattatggt cagcatgaat gaaggggtgt
    28981 tggcaattga ccaagacgca caagttttac tggccaaccc tcaggctaga gagcttctgc
    29041 aactagaaag cctggatctt gccggacaaa agattacgca gctcttgccg cacgctgcta
    29101 tggatgaact ttttatggcc gtgatcacca agggcgaagg gcaagtgcag gaatatgctg
    29161 ttgacggggg acgaattttg tcactgcaaa tagcaccgct gcagcgtggt aaaaagcttt
    29221 ggggagcagt aggtattttg caggatgtga ctgaagtacg gcagttagaa gaaatgcgcc
    29281 gcaattttgt cgccaatgtc tcacatgaac tacgtacgcc gatgacgtct attcagggtt
    29341 atgtagaggc cttattagat ggcttggcgg aagataagga aagtcagcag cgttatttac
    29401 aagtaatttt agatgaaaca attcgtctta atcgtttagt caacgactta ttagacttat
    29461 ccaggctaga aacgagacaa ctagcttggc ccctagagcc aatcgacctg cgtcccttat
    29521 tagaggaagt ctctgccaaa ttgcagccac aatttgccca gctagagcta acgacagctc
    29581 tagaagtacc ggtgcagtta cctttagtcg ttggtaatcg tgatcgtatc caacaagtgc
    29641 tgattaattt actgagtaat gctatctcct ttactcctgc tggcggtaca gttactcttg
    29701 ctgctgcaga agcagggaag tgggtgcgtg tcagtgtgca ggacactggc cttggtattc
    29761 ccgctgccga gcaggaaatg gtgtggcagc gttttcataa ggtggacaaa gcccgcacac
    29821 gtaagttcgg cggcacaggc ttgggactat ctattgtcaa gcagattgtg gaagcgcacg
    29881 gtggttctgt ggggctggag agcgctgtgg ggcaaggttc cacttttttc tttacgatac
    29941 ctttagctca ctagttacaa taacacataa agagaaaaac taccccttgt ttacagagtg
    30001 ggtagttttt ttgcgtactt ttgctgttcc caacttggca aaattatctc aacatctgta
    30061 atgaaggaat ttaagactaa tcaaagaact atactttgag gaacgtttcc ttattttatt
    30121 tcctggcagc aggacaagca ctgaattacg atatttttgt gagctaataa gagctttgtt
    30181 aactcaggcc gtggaaaaat gcataaacgc tagcaattct gcaataattc gtataataag
    30241 cagtaattgg tatataatat ttgaagcatc ttcgtcttta tcactgataa aagaaggaat
    30301 caatcaagca atgtagaatt aaaaatgtag taattactgg ctaagtttga cacagttacg
    30361 ggtgttagac aagggaggaa aaagaatcag cacggggtgc agagaggcct aacgttggtt
    30421 aaaacgctag acgagacctt gtctctcgaa ttagggatga ggttttttaa atggttacct
    30481 cttcttacag cgttactagc aggattttat tcactaatgg cgaaataagt aacccttgac
    30541 tagcctagcc taaaagcgcg acatgcttga tagcagttat tagatgtagc tcctcctaca
    30601 gggcagataa gaaacgcagg tgagacaggt gcaaaagctg ccaactgagt ttattgacga
    30661 actgcggact cattatgata tcgtggagct aattgctcaa tatgtgcagc tgaaaagatc
    30721 tggccgtaat tactttgggc tttgtccttt tcatgctgaa aaaacaccat cttttagtgt
    30781 ttcacaagat aaacagattt tccattgttt cggttgtggt gagggaggaa acatttattc
    30841 ctttttaatg aaaatagaag gtctgacctt tccagaagcg gtacgccaac tagcggaacg
    30901 tgcagggatc cccttgccgc agatcgctag ctcacccgtc ttgcaaaaag cagaattagc
    30961 taaaaaccgt cttcgccaaa ttatgcagct ggctaaccgc tattttcagt acaaactaca
    31021 gcagcctgga ggagaagcgg cgcgtcaata tctgcaaacg cgtggagtta ctgaagctgc
    31081 aagagaaaac tttgctttag gctggacacc agatgcctgg cgggaactaa aaacattctt
    31141 attacgtaaa ggatatcagg aaaaagagtt attaacaagt ggtttgatta ttgccaataa
    31201 aaatactgcc tatgaccgct ttcgcggtcg catcatctat cccatcgcta atccgcaagg
    31261 tgaggtgatt gcctttggtg gtcgagcgct aggtgatgag cagcctaaat atttgaattc
    31321 tccagaaacc ccgatatttg ataaaagtaa aacattgtat gccttgcatc ttgctcgcga
    31381 tgccatacgc cgcaatgagc aagctataat tttcgaaggt tatatggatg tcattgcggc
    31441 acaccaagct ggcatcaggc aagcagtagc ttcgcttggt acctctttaa cagagtcaca
    31501 agcaagactg ttacgcaacc aggcaaaaga agtagtgatt gtttatgacg cagacgctgc
    31561 tggacaagcc gctacttggc gtgggttgca aattttacga gaagctggtt gtttggtgaa
    31621 agtgggcatt ctgccacaag gattggatcc tgatgattat ctgagacgtt ttagtggtga
    31681 agcattccaa aaagaagtta ttgaacaagc tctgctgctc gttgattatc aacttaacag
    31741 tttggctgaa caatataata ttgaaaaaga cgatgagagg atacgtctct ttgttaaaat
    31801 tatagacgtt ttagccgctg ttgacaatgc catggagcgt gaagattatg tgcagaaggc
    31861 ggctaattta ctccagcttc ctcccacagc gatacgtgaa gaattagcta aaaagaaaca
    31921 gcctgtcaca cggccgcagc aacagcgtaa atcttggcca aaacaggcag ccacggaaaa
    31981 agcgccttta caaatcctcg tattatgggc tcgctttccc ttcctgattg cagaaactgc
    32041 tgctaattta gacagcgaga gtattccgga agaactgcgc gaagtcttta ccgcagcaca
    32101 aataactgcc gctttttcgc ccgcgctttt actagattta ttgccctcgc aaaaataccg
    32161 gcaactaatc agccgtttgt tgatagaaga tgattatgat gaaaagactg ccagcaaagc
    32221 attagcggat tgtgtgaaat atttgaaatg tgtccatatc gcgcaacagc gcaaggaggt
    32281 tgaagcgcaa atggcgaaat tggatgctgt cgccgcgaaa ggggaaatta ccgagttgtc
    32341 aagaaaatgg ctggaattgc gtaaaatgga agaaagcata aatcaagcca aggaaggagg
    32401 gacaggtgtt ggataaaaaa gttacacaaa agacggaaaa tgataaaaat caacagttaa
    32461 agaaaatccg cgagcaacta attataaaag gcaaaaaaag tggacttctt acctataaag
    32521 aaattattga agcgctgcag gaattggatt tatcgcctga agatattgat gatttctatg
    32581 accacctctc ccatcttggt gtagatgtag tcgatgatac aagtgaagaa gaaggcgtag
    32641 agcctcccga agacgacgac aaaaatgaag acagctccga aaccgttacg aaaattacag
    32701 cagctgaggg aattagcgtt aatgatcctg tacgcatgta tttaaaagag attggtaaaa
    32761 tcccactttt aactgctgaa caagagatag aactggcctt gcgtatggag caaggggaag
    32821 aggaagcaaa acgtgcttta gctgaggcta atctgcgttt agtggttagt attgccaaga
    32881 agtatgttgg ccgggggatg ctctttttag atttaatcca ggaaggtaat ttaggactaa
    32941 ttaaagcagt agaaaaattc gactaccgta aaggttttaa gtttagcacc tatgctactt
    33001 ggtggattcg gcaagctatt acccgtgcaa ttgccgatca agctcgtaca attcgtattc
    33061 ctgtgcatat ggtagaaaca attaataaat taattcgtat ttcccgtcag cttgtacaag
    33121 agttgggtcg tgaacctaac cctgatgaaa ttgctgaggt tatgaatatt accgaagacc
    33181 gagtgcgtga aatcctgaaa atcgcccaag aacctgtttc tttagaaaca ccaattggtg
    33241 aagaagatga ttctcatttg ggagatttca ttgaggatca ggatgtccag gcaccagctg
    33301 aagccgcagc atttgaattg ttaaaagaac agcttgatga tgtgctggaa actctgacgc
    33361 cacgcgaaaa aagggtgcta acgctgcgtt ttggtctcga ggatggtcgt tcccgtaccc
    33421 tggaagaagt agggcaagtt tttggggtta ccagagaacg tatccgtcag atcgaagcca
    33481 aagccctacg caaacttcgt caccctatgc gcagtaaacg acttaaagat tatctcgaat
    33541 agatcccaga aaacacctga acttaaaggg ttgacttgta cggtatttat acgctataat
    33601 aatcaatgca ccacatgaga taactttcct cgatagctca acggtagagc gaccggctgt
    33661 taaccggtag gttgtaggtt cgaatcctac tcggggagcc aagggcccat agctcaacgg
    33721 ttagagctcc cggctcataa ccggtcggtt ctaggttcga atcctagtgg gcccaccaat
    33781 tacaaatttg gccccttggt caagcggtta agacaccgcc ctttcacggc ggtaacgcgg
    33841 gttcgagtcc cgcaggggtc accatataag ggcgattagc tcagctggga gagcgcctgc
    33901 cttacaagca ggatgtcggc ggttcgatcc cgtcatcgcc caccattgta aatttaagca
    33961 gccatgatgg ctgctttttt agtgtctcat taaacaggct acggtcctta tacaatctta
    34021 atattttaac ggcaaatctt tgtagtttac ttatatttga ggtgatggtc tttacacaat
    34081 cttaatatgg aaaggggtag aatgggggca gataggaagt gagaaggtga aaacatgtgg
    34141 cttctgctaa ttatgctttt tctatcccct tttattgtca ttggttatct tgtcgctagg
    34201 ttagatcgat ttttggctgg cggtggcatc gttaaggacg aggtggaggt tcatccagcg
    34261 gcgattgttt tcggccagag cagcttagcc caagaggtta caaaaatatt gcataaaaac
    34321 aaagtaagag ttttcaccat acaggaacca ttcatcttag cacacaaaga taatgttcaa
    34381 ttcttatttg ccttatcaga gaatgacgcc gataatcttg ttttatgcaa aattggcaaa
    34441 aggatctatg ggatcgagaa agcgatttgc ttgtgtaacg agcagattaa caaaagattg
    34501 tttggcagcg aaaagattag ttttttatct ggtgaagaag caacagcctg cttgttttat
    34561 caaactgtct tacacaaggc ggaggtgaag ccgtgaggga gctgcagcta aaattaaaat
    34621 tgaaatcgtt cacttatccg caaatgatag cccttggtta tttaatcgtg attaccatag
    34681 gcacattgtt actgtcttta ccgctggcaa actacaacca ccaatcacct ggaattttca
    34741 atgctctttt tacagcgacc tccgctactt gcgtgaccgg attgattgtt tttgacacct
    34801 atactcaatg gtcccttttt gggcagttag taattttgct gctcatccaa ataggtggtt
    34861 taggctttat gaccatcatt acaatgtttt cctttctgct taaacgcaag attgggctta
    34921 aagagcgagg gttattgcgt gaatgtgtta acaccatcta catcggtggg gtggtacgtt
    34981 taacaaaaag aatactgctg gggactttat tgtttgaagg actcggagcc gttattttag
    35041 ccattagatt tataccaaag atgggaatag cagcaggaat ttataatggc atctttcatg
    35101 cagtttcagc tttctgcaat gccggctttg atttgatggg taggtatgga caatactcgt
    35161 cactaactgc gtatgcctct gacgctgttg tttgttttac tattatgctg ctaattatta
    35221 ttggtggtat tggatttttc gtgtgggatg atttagcgaa aaataagttt tacgtcaatc
    35281 gatatcaact gcacacgaaa atcgtactgg cagccactat cagtctgatc atggcgggct
    35341 cactttcctt tttcctccta gaaagaaata atctatttgc tcacctggga gtaggggaac
    35401 aaatcattgc ttcattcttt gcggctgtta ccccacgaac agcgggtttt aatactgttg
    35461 aggtggcagc gttagcacct gctagcaaac tattaactat ggtcttaatg tttatcggtg
    35521 gcagccccgg atccacggct ggtgggatta aaacaacaac atttgcggta atcttgattt
    35581 ctttgtggtc aagtttgaaa aatgctagga gtgaaaacat ttttgggcga cggttagaag
    35641 aaaatgcgtt gagacgagct tcggccgtcg tgagcttcaa tctgttacta gtctttagtg
    35701 cggtgctgtt aattagtgcc acaaatgtgt ctgtaccgct cagcaatgtg ttgtttgagg
    35761 tcctttctgc ccttggtaca gttggtctat ccgtcggggc gacaagtatt tttaatcctg
    35821 gggcccgttt agtattagtt ttattaatgt actgcggccg tgttggtagt atctcttttg
    35881 ctttgctatt tacagagcat ctcaaaccgt catctgtgca aaagccgacg gcaaaaataa
    35941 gtataggtta ggtgtggcat atgaaatcaa tgctagtgat tgggattggc cgttttggaa
    36001 aacatttaag tcgcaaacta attgaactta gcaatgaggt tatgattgtc gataaggaag
    36061 aggaaaaact tacggaactg ttgccgctgg ttacaagtgc ccagataggt gattgcacaa
    36121 atgaggaggt tctgcgctca ctgggcatta gtaactttga tgtgtgcttt gtttgtgtcg
    36181 gtagtaactt tcaaaatagt ttagagatta ccagcctctt gaaggatcta ggggctaggc
    36241 atgtaattag caaggctagc agggatattc atgttaaatt tctcttgcgc aatggtgcag
    36301 atgaggttgc agacccggaa cgggatattg cggaaaagct agcagtacgc tatagtgcaa
    36361 ataatgtctt tgattatatt cagctgacaa aagatgtttg tattattgag attccaccct
    36421 tggataagtg ggtcggccgg agtatcaaag aggttaattt tagagttcgt tatcaggtta
    36481 atattttagc aactaaagtt ggtgaaatgg ttttgccctt gccctcagca gaccatatct
    36541 tcaaggctga tgagcatctg atggtgctgg gccaccgcag tgatgtggaa aaaatattaa
    36601 aattactaaa ttgagtggta caatagctgt gaggtggcac gatgagagta aaatactatt
    36661 tacgcttgtg cgcgattatt attggtacaa cgattgcttc tcttgccttg agccaattag
    36721 gggtcaacaa ggaaaatatt ctcatggtct ttatcgtggg cgtattgctg gtcactgctg
    36781 tcactaacgg ttatcactat ggtatcgtgg cctcctgtgt cagtgtgatg atctttaatt
    36841 actttttcac gcaacccgtg cacactttcg ccatttctaa tactaatgat ctggcattga
    36901 tatttttctt tttaagtgct tcggtcatct cttctagctt aaccgccagg tttcaacgtc
    36961 agttattaat ttcacaaaag aatgaagaaa ctgccaagtt attatacgat gttgcacagc
    37021 gactgcttaa tgtcacaggt gaaagcaaca tcattcagca gggaatgcgc tgtattaagg
    37081 aatatactgg cgatgacagt aaagtcgaat taaatgggaa caaccaaact tattacagtc
    37141 ccaactttgc gcctgcacaa aatgaaaaaa gtattgagct gccgattatg gggttagcaa
    37201 aaaggttggg cacactgatg gtatataatg ttaagcttac gattaaacaa gaatggctgg
    37261 ttaaaacagt atgtgcgcaa atgggtatcg ccttagaccg tgaatttgtt tataatgaac
    37321 gcgagacgat tcgtattgcg atggaacgtg aacatttaaa aagcaatcta ctgcgcagca
    37381 tcagccatga tttgcgtaca ccgctgacag gtattgccgg ggccagcagt tatctggtac
    37441 agcgtggcag cagtctggag cgagacgatt atgagcgttt agttaaggat attaatgagc
    37501 aggcagtatg gatgacaact ttggtagaaa atatgcttaa tatgacaagg attgataacg
    37561 gtaagttaga ggtcaagaaa gaaattgaag tggtagatga tgtcgtcggt gaggctattt
    37621 cccatgtcac cgggcttgat gagaggccgt ttaaggtaac tttaccccaa gaactagttg
    37681 ccctgcccat ggatggtaaa atgatcgtgc aggtattaat aaacctttta aataatgccg
    37741 tcacgcatac acccaagggt accccgatcg agttgtcagt aaaacagggg gagagttatg
    37801 tcgagtttag tgttgctgac ggcggtaaag gcctagcata cttaagcgtg gataaaatat
    37861 ttaattcttt tgtcactagc ggtaaagtag gtgccgacgg taaaaagggt attggtctcg
    37921 gtctagctat ttgcaaagct atcgtccagt cgcatggtgg tagtatccaa gcgggtaagt
    37981 ctgaactggg aggagcgttg tttagtttta tcctgcccac ggaggtggaa taatgacgga
    38041 gcatactaca gtactcgtag ttgaggatga caaatatatc aagtctttag taacaatgtt
    38101 gttaaaagat gaaggttata aaacttttac ggcagccact ggtaaagaag caattgcttt
    38161 gttttatgcc aacaatcccg acattacatt gcttgattta gggttgcctg atattgatgg
    38221 catggaagtg attagtcaga tcagggaaaa aagtaatgca ccgattattg ttatttccgc
    38281 gcgggaagag gaaattgata aaactagtgc gctttactgc ggcgctgatg actatatgac
    38341 taaaccattt tatcccggtg aactagtggc gcggattagg gttgcccaac ggaaaatgaa
    38401 taatgcgatt aacccagata atgtggagca atttaccttt gataatttaa ctgtagatta
    38461 cactaaagga ctggtctttg tagatgaaca agaagtgcat ttaacgccca tggagtataa
    38521 actattaagg atattaattg ccaatcgcgg taaggtttta acacataatt ttattattaa
    38581 gcaaatttgg ggttatggag aagcgggcga cgccaaaaat ttacgtgttt ttatggcaag
    38641 tctgcgccgt aaaatcgaaa aggatactgc taacccccgc tttattttaa ctcaagttgg
    38701 cattgggtac cgttttgcca acaaataatt ctcactatcc caactcgctg ttgtgatgct
    38761 gcgatatggt gagtgcgccg aattcacatg tgatgcataa gctattagca taggcaaaag
    38821 cttatagtag aacatgcgag ggggtgtagg tatgagaacg ataccggcac taatagtcaa
    38881 atatgtttta actctggctg cggcttacct tgctagttat tttgcgggag gcgtttattg
    38941 gggtctgatt attgttctgg cactgttggc tgctatagca aactatgtac ttgctgattt
    39001 aattgtcctg ccggtggcca gcaatttagt cgcctccctg gtcgatggag gggtagcagc
    39061 gctgctgggc ttctttatgc tagcgagaga tacttcactt aacctaaaca cagtttggtt
    39121 tgtttttact tttgctttga tagttacagg atgcgaatat ttcttccata tttatttgca
    39181 aacgcaagaa aagagcacca actcagagta gtaaagcgcc ttggctgggt aaaaagtgaa
    39241 cagtggctca aaagaatggg caaatactgc acagtggttt aagcaggggg gaacgaaccc
    39301 cctgcttttt tggtggggct aaacttactt tgcggaggaa gagcagggcc gcagttattg
    39361 ctggcctagc gacggggtta agcgaaatat cccttatttt caaggtcttt gtcacattaa
    39421 gctggagcag tgttctgtcc ttttaagggc ggcaaaggat acacttgacc aattaggtaa
    39481 gttttatttt gtttggcatg aaacttgcac tataagttaa tgaaagataa tgaagtccac
    39541 tgctagtgtt aagaaggggg gatgcggcaa agtttcgttt tcagtgcaag acagagccct
    39601 atcggtgtcc tagtagtgac tgcaaatgta actaagaaaa agataggtgg tgcataagca
    39661 tgacagatac aaaagcttta aaagaggaaa gaattagtat ttatcacgat gtggctgata
    39721 acaagattcc taagagggtg cccattggtg tttcaatcgg ttttgaggca actatgcaat
    39781 actttggtta tgatttaaga aatatttact ggaaccctag cttattgaat aaggacgaac
    39841 agaaagctga cgaactgtgc caaaaggtat attctgatac tctacctttc tccacatcct
    39901 cgctgcgttt ccctactctg tatcaattta ttgagtcaca atcatttgtc atgagctcca
    39961 gtggcttcat gcagcatcct gaagttgttg gtatgctgcc tgaagattat gattatctga
    40021 ttgaaaaacc gtgggattgc attttagagc gaataattcc gcgtcaccat aaggccttta
    40081 atactgatga gcctattaaa atggccttga actttactaa aggtattttg ggttatctca
    40141 atgatccccg cgaaggggca gccctgtcgg caacaatgcg cgaaagatac ggttattatc
    40201 cgggtgcacc tgctggttct ggcggtttta cagcgatgcc ttttgatttt cttgctgacc
    40261 aactaagaag ctttagcggc attagtacag acgtcagaag aaggccagag caagttgccg
    40321 aagcctgcga agcactttat cccatcgtct tcagacgtgg tttgcccaag gttgtttcta
    40381 attatggtac ggtgtcaatt cctctgcata tgccgacctt tatgcgtgaa aaagactttg
    40441 aaaagctgtg gtggccgact gcaaaacgtt tagttgaaga gtatgcttcc atgggtattc
    40501 actgtaactt attctgtgaa gatgattgga tgcgttatct ggatcatctg tccgaactgc
    40561 ccacagacac taccctgcgt tttgagttcg gtgatcccaa attagtgcaa gagaaattag
    40621 gcgataaatt tatcatcagc ggcctgtatc ctatgcagtt aatcaaaaac ggcaccaagc
    40681 agcaggtcat tgataaagct aaagaagttg ttgatgcttt agcacctggc ggtaagtata
    40741 ccttcggctt tgataaaggc attcaagttg gcgccgattt aaacgtggag aacctagctg
    40801 ccttaacaga gtttctccag gagtatgctg tctacgataa tccaggagaa acagcaggaa
    40861 aacagtttaa taaaaatgat tataaagcac cgactgctgc ttcttataag attgacagta
    40921 aatatttcag cacctgggaa gagtataaag ctcaataccc caacatttct gattttgccc
    40981 aagacaaaat cgagaattac gaaaatcaaa tgcttacttt gctcacttgg ttgttaatgt
    41041 aagagtaagc tgaatataag attctccatc taacatgtac aaatgctgta caattgttta
    41101 caacagggga ccttatcccc ctgttgtagc aattggcaaa actgatcact aattgtttac
    41161 atgcaaaaac ggcagcgtat cccgaacatt aatcacttta gctgactttt aattaacatc
    41221 aatgagggag gtcataccat gtcttttcta acgtatggcg taggcgataa taaaagatgg
    41281 tattatccta tgtctgcttt tatgatgggc gcaatgcgca ttgcaccttg ggcactattt
    41341 tatccgcatg tccaaactgc atttaattta gaaacaacgg cgaatattat tttggtttct
    41401 acttttacag gactaggttc gatgattctg gggccgccaa tttctggtac gatattagat
    41461 aaatatggcc ctaaaatccc ctttgcttta tctggtattt tttccttagc tggttacctg
    41521 accttaatgg tcgtgttagg caaagaaact tgggcagagg cccaaaccct gtggtatgtc
    41581 ggcggctttt taattggtct tggtgccggc ttctatgctg gcagctacac agctacagtg
    41641 gcgaaatggt ttccggatcg gcttggcgtt gcgatggcgc ttggcggctc gggaatgagt
    41701 atcgggacta tgatcctgac tccactgatt gcctcgttta ttgtgagata cggttttacc
    41761 ggcaacatct ttatgatttt aggtattgtt ggtttttgtg ccattacttt gctcggagcg
    41821 attccctggc ggttaccgaa agctgattgg aagccagaag ggttaggcgc taaagctaaa
    41881 gcttctggga aacctgtgca agaacaaaaa cagtttactt ttgcggaagc tgttcgtgat
    41941 agaagatatt ggattctagc tgggggtttc ttgggagcgg cattttccaa tatgctgttc
    42001 tcacaaaacg tcacactaat tatcattgaa ggcctcacct ctgtaggcat gggacgtgca
    42061 gatatcatgg ccacagttgt tccttactac ctttccctga cctcctttgc cggtatcttt
    42121 ggtacattcc tgtggggttg ggtgctggat aaattaggtc cgtttgtcgc cctgccgcta
    42181 atgtacttca tttctggggt actaatctgg gttttctata tgggctatac cagtgttact
    42241 tttatctatg ttgtgggcgc tattctagtg ttggccatgc gcggtgaagg aaccctgcat
    42301 tatgccgcgg taccagcagt ctttggccgg cagcatattg gtaaaatcat gtccaccttg
    42361 aactccatgt ctgttggtat cggtattgcc ttggggccgt ttcttggctc ctatatccga
    42421 gatatcactg gtggttttaa aacttcactg attctcgctg tctcgttaag aatgatgggc
    42481 acagcttttg ctttagttgg ctacttttta accaagaagg aaaaagcgaa gaaggatgtt
    42541 acagcataag catcataatg tgtaggaccc tgccaatagc ggcggggtcc ttttatatca
    42601 tctgtaaaaa gtaggtctag tactaacatt gtgggaataa ttatcaagta tgttgaaatg
    42661 aataaaggac tttccatgaa ggatatagaa catttactaa agttcgaacc tttattgatt
    42721 aaatctgtgg agcattaacg atgcggtaat tgagacgtac aagcgtagat gataggagtg
    42781 ctgaaagatg tctctggatt cacttcgtaa acatgtaaac cgcgttaaac aagaatggga
    42841 aaactttttc gctggtaaag aggttgacgg agaagttatc cctaatgtga tcaaacgatc
    42901 ttgggtgcgt agtcgcgctt atggcgtaga tccactcagt acgctgaatg actcgtggga
    42961 gcctgaaata aagtgggcgg ctgaatctaa tccgctgctg cagttaaatg ttaattttac
    43021 taacccttat gatagtgcgg gctacatcat ccaattatat gatgcagcag gttaccccct
    43081 aattaactcc aaggccaccg ctcccatgat agaaaaaaga atgggtacta attcggtttg
    43141 gctggcttta acccaaaaca agttagtatc tgtgtttggc tatgaacact atcgcgccag
    43201 ctaccacaac acttatggtg cagcagcacc aattcatgga aatgatggtc aggttcttgg
    43261 tgtgatcaat gtggtatgca atgagctgga attagcaccg aatattgcat caatggcctc
    43321 acaatttgcc ggcgtagtgg aaatttccct atctttacta aatcgcaatg gtaagcaatc
    43381 tccgttagaa gcattctttt atcaatttcc gcaaggcatc gcctggtata gtgagaataa
    43441 attaatccgc tataatcaaa agattttaga tatgctgcag attaatgagc aagcggggat
    43501 acaagcagcg cttgagaaac agctgtcaat ttggcttagc ggacgcttga ttaaccgtaa
    43561 atcggttagt atgaatatct tgggtcagaa aaaagagttc tactttacct ccatgcctat
    43621 tgctactggc ccgggacggg gtaaagagaa attcctgctg ttagaggaaa aaggtaatcc
    43681 caaaggaagc cagcagcttg tccagcatgg tacaactctg gttgaatttg atagtattat
    43741 tggcgagagt aaacaacttg aagcgacaaa gcgtactgcc caaaaagtgg ccagtgccga
    43801 tgaaccagta ctgatcatcg gggaaagcgg tacaggcaaa gagctctttg cgcaagccat
    43861 gcacagagca agcatgcgta gtggtaagtt tgtagctgtt aactgtggtg ctattccggc
    43921 agaattagcg gagagtgaat tgtttggcta cgaacacggt gcctttacag gtgccttaag
    43981 aaatggtaaa gaggggctgt tggaatcggc ttctggagga accatctttt ttgacgagat
    44041 tgaaagtatg ccattattta ttcaggtaaa acttttacgt gccattgcta cagggcggat
    44101 tatgaaggtg ggcagcagcc aagaaacgcc tattgatata cgtgttattt ctgctactaa
    44161 aaaagactta ttaaaagaag cagatgctgg caattttcgt gaagatcttt attatcgcct
    44221 cagtactttt attttgcatc tcccagcctt gaatgaacga ggtaacgata ttattctttt
    44281 agcacagcat tttattaaaa agacacaaca aaaatacgga aaatacccat tgaaaatcac
    44341 cagagacttc tggcgagctt taacctgtta tcactggcgg ggaaatgtta gagagttgga
    44401 gaacattatg ggcagagttg tttttatggc cgagcaacaa acaataactg cggctgcctt
    44461 gcctgcgccg atacaggaat cattgaaact gcagcctgtg caagcagctt tggctgaaag
    44521 cgcagattta gaggaacatg cagacggact attgcaaaca ggtgaggaaa agattattgc
    44581 cgcagtatta caaaaaacaa actttaacat aagccaagct tcccagatct taggcatcag
    44641 ccgcaaaacc ctttacaacc gtattaatag tagtgagcga ttaagtgact taaagtctct
    44701 taaggcaaaa gtgtaggcag cgagcgtaaa ttaaaaaaat ctcgtagaag aataaaggat
    44761 aatcttcttt tctagcgaat tgtataattg ttattatata gtaacaattt gcactacttt
    44821 taatttttag atcgtgtttg attagcgccc atagctgttg ccaaagcgac agtgtatata
    44881 ggtatgggtc tcagcgtgtg ctgagaacaa cagcgccttg tgcgtgcaag ttaaaaaagg
    44941 aggattgtac atgttagatc ttaaagtttt aacagaagca gtgggagaac tggatgagga
    45001 aaaggtagtt agtgaactgg aagcattcgt agctagcaac ccaactgaac aggaagcaca
    45061 aaaagcggtt gcagcctgcc aagctggcat ggctattgtc ggtgatttat ttgagcaagg
    45121 tgaatacttt gtaggtgact taatttttgc tggtgaatta cttacaaacg ctatcaacgt
    45181 tctcaagcct gttctaggca gcggcactac tgacagcatt ggtaccctcg ttttaggtac
    45241 agttgctggt gacttacatg atatcggtaa aaacatctat aagagtatga tggaagctgc
    45301 tggctttgaa gtacacgatc tgggtattga ccagcctgct agcgcattta tcgagaagat
    45361 caaagaagtt aagcctgatg ttgttgggat gagcggcgtg ttaacattgg cccttgagtc
    45421 aatgaaagaa accgtggatg cactgaaaga agctggcgtt cgtgacagcg ttaaaatcat
    45481 cattggtggt aaccctgtaa ctaaggaagc tagtgagcaa atcggtgctg acgcctttac
    45541 aactaatgcg gctgaaggcg ttaaaattgc ccaaggttgg gtttcttaat tagtatagct
    45601 agctaataaa gtgacaaagg gaatattccc tttgtcactt tttcttactg ctgggaaatt
    45661 ttcttgtttt acacttacac tgattgttag cagtagagca ccatggttcc cctcgactgt
    45721 tggcaggaaa tacctgtctc tagcgagaaa atagatgtat acacctataa acctaaaaag
    45781 ataatatgaa ttaatatata attttaggta taagtggaaa aaatgaaggt ggaggtgtat
    45841 gcacattgaa actcaccccg cgtttacagg caattgctga attaattcca ccgggaagtg
    45901 tcatcgcgga tattggtaca gatcacgctt atttacctat atatctgttg ctggaacaaa
    45961 ttagtcaaag tgcggtggcc gcagatatta acgaggcacc attagaacaa gcacaagaaa
    46021 cagtggcagc ttttaattgt catcaaaaaa tagatttacg acttggtgac ggcttggaga
    46081 ttttgcaaga agctgatggc gtggatacta tcgttattgc cggcttaggc ggacgaacta
    46141 ttgcctccat tctagaaaca ggaagcgata aactccgaca acttaagcgg attattttac
    46201 agccaatgaa tgaaacaggt tatttaagat tgtttttagc taaaaatggt ttagctattg
    46261 tgcatgaaga gctagtttta gaaggtaaac atttgtatga gattattttg gctcaccctg
    46321 gccaggaaat agaagaggat cctttcaaac tttcgctggg acctcgcttg ttagagaaaa
    46381 agcctcccct tttaccatta ttattaaaag agaaaatccg caaattgcgt attgtttacc
    46441 atagtttaca acgtgcaaaa aagaaagatg tttcgggcaa aatccgtgat gtgcagaagg
    46501 aattagattg tctgcaggag gtgttagcag gtgcaactca acgtacagac actgattaaa
    46561 atattggaag agctagcacc taaacattta gccctggaat gggatcatgt cggcttgcag
    46621 cttggtagta tgcaaggcac tgtcaagaaa atttacgtag ctttggatgt taatgcagaa
    46681 gttttagctg aagctgtgat gaagggtgct gattttattg tcacccatca cccatttatc
    46741 tttaagccgt tgacgactat tcgtactgat caagaaaagg gtaaattaat tcagcaagcc
    46801 ctgcaaacag gcatccggat ttatgctgct cataccaatt tggatatagc atttggcggc
    46861 gttaacgatg ttttggcggc acgacttggc ttgcaaggta cacaggtttt acggatcacg
    46921 gaccaggagg agctgctgaa agttgtggta tttgtgcccc agcgtgatga gcataatgtc
    46981 cgtactgcga tggcggctgc aggtgctggg tgctggggaa attatagtca ttgtagtttt
    47041 tataccactg gtacgggtac ctttatgccc cagaaaggat cgaatcctta ccttggcgag
    47101 caaggccagc tagagaaggt tgaagaagta cggctggaaa ctattatccc tgccagctta
    47161 agtaagcgtg tagtgcgagc gatgctgaaa gcacatcctt atgaggaagt agcgtatgat
    47221 ttgtatccct tagcaaacaa gggaaaagag tacgggttag gtagaattgg taatctacag
    47281 cactcattac tattatcaga attctgtcat ttggttaaag aaaggttaga gattcctttt
    47341 gtgcgtgtga caggtgatac agataaacta attagcaaag tggctgtttg tggcggtgcc
    47401 ggcagtgact taattgctgc tgcttctttt gccggggctg aggccttggt aactggagat
    47461 ttaaaatacc atgaagccca ggaggcgcag gtcttgggcg tggcagttat tgatgcgggg
    47521 catgatggga cggaacgtat tattgtgcct gccctttgtg attatctgca gcaacagttg
    47581 gctgctgccg gttatcacat tgagatcatc gcttcgtcaa ttgatacaac actctggaac
    47641 ttattctaga caggtgaata acctgtcttt tattgtgctt ctatgcttat accttttgcc
    47701 atgaagtgat gcagcatggg gacagtaaaa aataaaaagc ttgccctggg aagctaaaaa
    47761 acgcaagctt tttaatctaa ataaggaggt tgctaggatg ggagaattac aaaaacttta
    47821 cgagctgcag caagttgaat taaagattga gagtttgcaa aaaaccttaa aagaactgcc
    47881 cgtttttgct gcatttaaac tgttgcaagc tgaaacagcg gatgctaaag aagcattggg
    47941 gtgggctgaa acaaaactaa ctgaacagga taagcgtctt caccgtttag agatggatct
    48001 acaggatgct gaagcagagc aggagaaagc acaaactcag ttgtttagta atagtaatca
    48061 gagcagtaag gggttagaac aactagagcg gcgcagtgat gctttaggca aagaacgtct
    48121 ggaaaaagag gaaatgctgc tcttggcgat ggaagggaaa gataatctag cgcaaacact
    48181 gcaaaaatca aaggatgatt atcggtcacg gcggaagaaa ttgcgccagt tacagaaaac
    48241 aggtaatgct gaaatccaag agttgaaaaa caaaattaat gacttgcaag agcaacggaa
    48301 gcagctgcag gaacaaatca gtgaatccct tttaaaggat taccgtcagc agcgcccacg
    48361 ttataacggt aaacctttag cgcatctgga aaacgattgc tgcagcggct gccgtgtgat
    48421 ggtttttaag acgctgaaaa gtaatttaaa tcaaccaaat agtaaagtct tttgtgagaa
    48481 ttgcggccgc cgtttagtgc cctaaagtgt aaatccattc atttcctttt gacttttgga
    48541 caaaggaatg ctaaactaga taatcctgag acaatttcat ataaagtagg tcggatagtc
    48601 gcggggacaa ttgttctcga ggaaagtccg agctccatag agcagggtgc tgggtaacgc
    48661 ccagtgaggg tgacctcaag gaaagtgcca cagaaagata ccgccttcgt gtgaggtaag
    48721 ggtgaaatgg tgcggtaaga gcgcaccagc agtcaggtga ctggctggct aggtaaaccc
    48781 cacctggagc aagaccaaat aggggagagt tgacgttgct cgccgatctc ccgggttagg
    48841 tcgctagaga tgttaggcaa ctaacatccc agagagatgg ctatcgtccc gtaagggaaa
    48901 cagaactcgg cttacagacc tgctttatac aaagttccat agacactacg ctttaagcgt
    48961 agtgtctatt ttgtcatgga catagaagca actttataag ccgagtttga gtttcaacaa
    49021 ttcctgttgg gtaaattcac ctttgtaaag ccttgttaaa aaggcgctgt aaacaccgta
    49081 ttttatatat ttgcacacat tattcttggg acatttttac tccccaaatt gcaagtagat
    49141 ttaagattgg cattaggtct tcacaggcat ccgttaaaga atattctact ctcacgggaa
    49201 ttccatcata ctgttttcgg tataataagc catcatttac taattccttc aaagaactgg
    49261 ccaacattgt gttcgttatg ccgcgaattt tacgttttag ctcattatat cttgttgttc
    49321 cgtcgtggtg taaggcacat aaaatgggtg ttttccactt tccgcctatc aacttcaatg
    49381 cgttccctaa cggacagtaa tttttacaag ggcaattatt gctactgaga ctttcatagc
    49441 aattatttag ttctgtaatc atattttccc tacctactca ttatattctg cgtaattgaa
    49501 aatgaactat tgcagtagta ttattatacc aggtagtaaa agcaaagtta agagctgcca
    49561 aacaatttta ggaggtaaaa ttatggttat taatgaggaa gtaaaggaag ttctcgaggg
    49621 ttcggcattt ctaacgcttg ttacggtgaa tgtagatggc acccctcacc ctattattgc
    49681 gggaaaagga caagttgtaa acgatacagt agtctttggt atttacaaaa tggtgataac
    49741 gcagaagaat ttgatgtcaa acaaaagtgc ctgggttgta ggggcgacaa aggctgaagg
    49801 tgctaaaggt tatcgactca caggtacagc ggaagcgaag gataagcagc ttattttcac
    49861 tccgacaaaa atagatgttc tgctctaaat attaataaac atatatcaaa aagggaagaa
    49921 gatactatca aaacaactag agtacttctt cctttttctt tgttatgatt taacatgaca
    49981 atgtttatgt tgccgatgat tttaatagga atttcttaaa aaggaataag caggcatctt
    50041 ctggcaaaca gaaaaacctg cttatttttt gtgcgaccaa agctttctgc ttgcgctgct
    50101 agaccagaca gttcctattt tgtggctcac aacttgctca ggaagtaaat aattatggaa
    50161 aaggtaacag ccctttatca aggcgacaat tagttaagca ggaagtatct gaaattcgtg
    50221 gaattgagta ataactggcg aaagatgagg gaggtttttt atgctggaga ctatactatt
    50281 agtatttgca attctcaatt tagtagttgt actgttgatg ctgcaaaaaa tggggcacgt
    50341 ggataatcgt gaacttaagc agatgatgcg tgaagagata gctgctttgc gcaatgaaat
    50401 tactttgagt gcgcgtcaaa atcgcgagga attaaacgcc tctgtgaggg cttttgggga
    50461 ctcactttct aaacaaagca tggagatggc gatgctgcaa aaagttcagc tggatacact
    50521 gaccgccagt aatgaacagc ggttggatgc cctgcgcaaa agtatggagg caaagttgac
    50581 tcatttgcag ggtcaacaaa atactgatgc tttgcaaaac cgtactgaac tggcaaaatc
    50641 gctgcactca tttgaggaaa agttccatac cagtgtcaat gagttgaatg ctacgcaacg
    50701 acagaagttt agtgatttgg cggagcagct ggagaagctt gtacaaacca gtgcagataa
    50761 attagaaaga atgcgtggta ctctggaggc aaaactacaa gagctgcgca cagataatac
    50821 agaaaaacta gagatgatca gagccacagt ggaagaaaaa ctgcaggcta cgctggaaaa
    50881 acgtttaggt gagtctttta aacaggtcag tgagcggctg gaacaggtgc atcaggggct
    50941 aggtgaaatg caaaatttag ccacaggtgt gggtgatttg aaaaaggctt tagttaatgt
    51001 taaagtccgc ggtacgctgg gtgaaattca gctggcgagc attcttgagg aaatcctaac
    51061 gccagaacag tatgcggtta acgtggctgt gcggccgggc aggaatgagc gggttgagtt
    51121 tgcgctgaag atgcccggta aagatgaaca tcctgtttgg ttacccattg atgctaaatt
    51181 tcccatggaa gattatcagc gcctgttgga tgcttatgag agtgcggact tggcgcaagc
    51241 agaagcttgt gcgaaacagc tggaagcaag tattaagaaa tgtgccaagg atattcgcga
    51301 taaatatcta gacccgccgg caacaacgga ttttggcatc atgtttttac cctttgaagg
    51361 gttatatgcc gaggttttac gccgtaatgg cctctttgaa acactgatgc gtgattataa
    51421 agtagcaata acagggccta ccaccttagc agctttctta aatagcctgc aaatgggttt
    51481 tcgcacctta actattgaaa aacgtaccag tgaagtttgg tcactgctgg gggctgttaa
    51541 aactgaattt ggtcgttttg gcgctttgct cgataaaact agaaaaaaac tgcaggaagc
    51601 cagcaatact attgatgttg cttcccgtaa atctcgtact attgaacgga agctaaaaga
    51661 tgtgcaagaa ttacctgatt tagaaagcga acaggtatta ttgccagttg aggagaatag
    51721 ttgaagttaa aatattgtgc cagagagaaa gttcagaaag gtcagtaaaa agtactgctc
    51781 ttgtgcagga aattgttagc ttatgtcgaa tcatagctaa tgcgctcttg ccagaagcag
    51841 aatttaggag tttatcatga tcaaaaaaga actagttaag ttattgtttg atgcagcgag
    51901 tattcagcgc tggaatgatc acaatcgtcc ctataaaggt tttacggaat tagataagca
    51961 agctcaaaag atggtttacg cttatttgct agctaaattt gaacagtcag accatgatgt
    52021 ttgtgtcaat tggcgtcagt taatcgaagg tggatttttt gaatttttac atcgcattat
    52081 tttgacggat attaaaccac caattttcca taaattaatg gccgaaaaag gtgatctttt
    52141 aaatcagtgg gtcattacac agctaacgga agcattagca ggggtaaatg acaatttttt
    52201 agagaagttt aaagcttatc tgtttcagcc agagtatagt gcgctggaga aaagtattct
    52261 taaagcttcg cattacttgg cgactaactg ggaatttaaa ctggtctata ctttgaatgc
    52321 taatctctat ggtttggaag agaccaaagc taccattgag gatgagatcg aggaacacta
    52381 ccatttagcc ggtgtgcaaa aattacagct tggcaagaaa acaagtcatt ttatggattt
    52441 agtagggcag ctgcgttttc aacagcgctg ggcgcagaca gctaggctgc cggaaacttc
    52501 tgtcatgggt catatgctga tggtcgcgat gctttcgtac ttggtgagca ccgagttaaa
    52561 tacctgtgat cagcggttag ttaataacta tctggcagga ctttttcatg atttgcctga
    52621 agtgctgact cgtgatattg tctcccccgt gaagcgttct gttgttggct tggacgaact
    52681 aatcaaagat attgaacagc gtcaggtaga ggaaaagctg ctgcctttac tgcctgcagc
    52741 atggcacaag gaactgcgct actttacaga agatgaattc gccaataagg tcattcttga
    52801 cggaaaggtt actattgttg aaggcagcac catcggtaac cagtataatc aggatcgata
    52861 ttctccgctt gacggtcagc taatccgcgc ttgtgatcat tttgctgctt acatggaaac
    52921 ctacctatcg atttctcatg gcattaagtc tcattttctc gaagaaggat accaacaact
    52981 tttgctgcaa tatcaggata aatcagtatc tggttttccc ttttggcaat ggttcaacta
    53041 ctttcgtcac ccagagacaa actaattgtc tcttaattac actaaacaaa ctacactgga
    53101 ggttttactg ctatgtctaa tgaatcgttt aaccttgaag cctattttaa acgcattaat
    53161 tatcagggga aaacagatgt tacggcggaa acactcaagg gtctgcacat tgcacatacg
    53221 ctgaatgtgc catttgaaaa cttggatgtt cattttaaga aacctattcg tttagaccgt
    53281 gaatctctgt atcaaaagat agtagagaat aagcgcggtg gttattgctt tgaaatgaac
    53341 ggcatgtttt cttttgtcct gaaagagatg ggttttaagg tgctagatgt cttagcgcgg
    53401 ggaaccagag acggcaagga atttttttct caatcccacc aagtgatggt tgtggaaatt
    53461 ggcgacgaac tatggctagc tgatgttggt ttcggcaatg atggtattac ggcacctgtt
    53521 ttattagaag aaggggtgga tcaacagcag ttcactcata cctaccgtat tttaaaagac
    53581 cccaaacacg gttatatttt acaatctaaa gtagacggtg agtataagta tatgtatgct
    53641 tttcacctga tcaattgtca ccctatggat tttgaaatgg ctaattattt tatggccacc
    53701 tatcctaatt ccttcttcag tattatgaaa atgtgcacga tgccgactaa agaagggcgg
    53761 gttaccctga cagatatcag aaatgaaaag aaatttaagc agcttgtcaa cggccaggtg
    53821 actgaaacta atctcaatag tgaggatgaa tttaaggaaa aattacaaca gtattttgct
    53881 ctggattatg aggcaattaa gcagtctgct gcaaaataac cacagcagat caagagagtt
    53941 tagtgcgcct agcgtaacaa caagaaagca gataataatt aacggagcgc agccgcgctc
    54001 cgttaattta tggtgtgatg gttagttatg tcagattgcc ctagatggcc atgtgttaag
    54061 agaccgtaaa ggtcaagccg tcgtcactag catctaccaa gacgatacta tgttctgtca
    54121 gctcgccggc aattagcctg cgggctaaag ttgtttccat ttctttttgg atataacgtt
    54181 taagaggacg tgccccatat acaggatcat aaccagcttg ggccagcctc tctttggccg
    54241 cttccgttaa ctccagtcct atatagcgtt ctgccagccg gttacgcaat aaagtcagct
    54301 gcagctccac aatttgtttg gtttctgcca gagtcagagg tttaaagagc acaatgtcat
    54361 cgacgcggtt taaaaattca gggcggaaat ggctccttaa ttcagccatg acttgctctt
    54421 gaactgtgtc gagaatttca ccgctgctgg taacattctc taataagtag atgctgccaa
    54481 gattagaggt catgatgatg atggtgtttt taaagtccac agtgcgtccc tgactatctg
    54541 taagccggcc atcatcaagg atttgcagca gtacattaaa aacatcatgg tgagcttttt
    54601 caacttcgtc caacagtaag acactgtatg gtttgcggcg caccgcttct gtgagctggc
    54661 cgccttcttc gtaaccaaca tagccggggg gagctccgat caggcgggct acagtatgtt
    54721 tttccatata ctcactcata tcaagccgaa tcatattgtc ctcactatca aacaatgctt
    54781 cggctaagga gcgggctaat tcagttttac caacaccggt gggtcccaga aaaatgaagg
    54841 aaccaatcgg ttttttcggg tcttttaggc cagagcgggc acgtagcacg gcgtcggcaa
    54901 ctgcttgtac tgcttcctct tgtccaatca cccttttgtg cagtatttcc tctaggtgca
    54961 aaagtttttc tttttcgcct tctactagac gggaaagggg gattcctgtc cagcgggcga
    55021 ctacttccgc tacatcctct gttgtgactg cctcgttaat tagtttggcc tcgctgccga
    55081 ctagcttgct ttctgcttct tgcagcttct tttctagtgc tggtatcttg ccatactgat
    55141 attcggcgac cttatttaaa tcatagtcac gttgtgcctg ctccatttca gtccgcgctt
    55201 tttctagttc ctcacgtaag gcacccaagc cgccaatggc ctgcttttca ttatcccatt
    55261 gtgctcgtaa agtatcgcgt gttgtcttca gttcagctaa ttctttttct aaggctgaca
    55321 gacgctcttg ggagggctgg tcctcttctt tacgcagtgc ttctcgttca atttctaatt
    55381 gtaggacacg acgctccagt atttctagct cttcgggcat gctttccatt tctgttcgca
    55441 gctttgcggc tgcttcatca attaaatcaa tcgctttatc gggtaaaaag cggtcagaga
    55501 tataacggtg gctaagcagg gcagcggaaa caagcgctgc atcttggatg cgtaccccgt
    55561 ggtgtaattc atagcgttca cgtaaacctc gcaagatgga aatagtgctt tccactgatg
    55621 gttcttcaac taatactggc tgaaagcgac gctctagagc agcatctttc tcaatgtgct
    55681 gccgatattc gtccagggtg gtagcaccaa tacaatgcaa ttcacctcgt gctagcattg
    55741 gttttagcat attgccggca tccatagcac cttctgcttt ccctgcaccc accacggtgt
    55801 gtaattcgtc aataaaaaga attatccggc ctgctgactt tgtaacttcc tcaaggacgg
    55861 ctttcagcct ttcctcaaat tcgccccgaa atttggcgcc agcaattaag gaccccatat
    55921 ctaaagccac tagacgtcgg tctttaaggc tttcagggac atccccagcc acaatgcggc
    55981 gcgccagtcc ttcgacaatg gcagttttgc ctaccccagg ttccccaatt aaaacagggt
    56041 tatttttggt gcggcgagag agtacttgta tcacgcggcg aatctcttgg tcacgaccaa
    56101 tcaccgggtc taatttgttt tgtgcggcca tcgtcgtaag gtcacggcca tatttttcta
    56161 aggcttcata tgtttcttct gggttagcgc tggtgacccg ctggtttccc cgcacggcag
    56221 tcagggcttg gaggaaggtg tcacgtgtta agccttgttt tttaaagatt tttgtcatcg
    56281 ccgtatcatc aaacattgcc agcacaagat gttccacgct gacatattca tccttcagcc
    56341 tttttgcctc ctcttgtgcc tgcactaaca gttggttgag tcgctgcgaa atataggtcg
    56401 ctgcggcacc ggtgacggca ggcattttag agagggtttg ttcaagaacc tgctgtagat
    56461 tactaacact agtatctgct ttggttagga ggcgtgggat gagcccattt tcctgcagta
    56521 gtaatgccaa caagacatgc tccacgtcaa gggcttggtg acttctttgc accgccaaat
    56581 cctgcgcccc tgataacgct aattgcgcct tttcggtaaa cttattaaga tccatacgcc
    56641 acactccctt tttctaatct tctaacctaa tcgtaagtat tattccacat cttgtcaatt
    56701 aacaaaagct tatctttttg cttaatcaag attcagcaca agcttgaagg gatgagaatt
    56761 tgccacccct tcgtcggctc tgcgggtcct gaccaccccg caagattaaa agggaatcgc
    56821 ctgaaaaaca ccatcggcta agaaagatgc aattcaaggt tgatataatt ttttatccaa
    56881 taagaaaagc tccctgctgg gagcttttca cttgagagga gtgcgtctgc ttaaatttca
    56941 tggaagcagg agccgccgat gaactcgcgc aggatagagt tattagggat gccctgtagt
    57001 tccagcgatg caaagcagga cagaaaaagc agcagtcttt tcgcatcgat gtattccggt
    57061 tcagtgtgac tgatttcttg taaaaggggc gtagcaaact gttttaggat cgccagctcg
    57121 taaactaggg aagaattaaa cataatatct gcttcctctt gtaggtggaa cagattgcgc
    57181 tcttcaccgc ggcgtacaga gggccagcgg cgaatagttt ccagggcgtc atagctgcgg
    57241 aactgatgat cgcgaatgat gcggcgtaat aaacgcgtgt cagtagtgtg gatgcgggta
    57301 tgattatcta cgttcagtgc ggttagggcg gaaatataga ttttatattt gtggcgacgc
    57361 gaaattgctt ccgttaggcg ttcgttcaag ccatgaattc cttcgatcag gaggagttgg
    57421 ttttcttgca gctcaatcgt gtgaccatga tattcacgtt ttcctgtatg aaagttgtat
    57481 ctggggattt ctattttttc tccgtcaatt aagcggagca gatgattgtt aaatagttct
    57541 aaatcaatag cttccagatg ctcaaaatca ggttgattat tttcatccag gggcgtatgt
    57601 tcacggtcga caaaatagtc atcaagtgaa atggggaaag ggcggattcc gttaactagc
    57661 agctgaatac gtaagcgctg cgtaaaagtt gttttgccgg aagaggaggg accggcgatc
    57721 aaaacaatgc gcagttcgtc tttacgtgct gcaatggaat ctgcaatttg ggcaattttt
    57781 ttctcatgca aggcctcatt aatgcgcata atttccggtc cttgccctgc ttcaatgcgt
    57841 tcattgagaa taccaacagt atggaaaccc aaaatttcag accattcttc agtttcgcga
    57901 aagattccga agagcttgtg ttgttcaata taggggggaa tggtaaaagg atcggcggcg
    57961 ttaggaaaac gcagcactaa acccggtgca cgaaaatgta cggcaaacca ctgcaaaatt
    58021 cctgtgcgcg taaccatata accatgtagt gaatccgtta atgctcctaa agagtagaca
    58081 gttagtgcct ctgtcggcca gtttttatag agacgtactt tatcatcaaa tcctgcttgc
    58141 ttaaaacagt caattgcttg ctgaagcggt atttttttct tttcaatagg caaatcagct
    58201 aaaaccagct cccgcatttt gtgctgcagg gcagccacat cttcagtggt taaggcaggg
    58261 gttttattaa tctcacaata aagcccttta cccaaggaat gttccacact gatctgggcg
    58321 tcaggaaaaa gaagcctcgt cgcataaatg agcaggaagg taagactacg ctgatagatt
    58381 ctggctcctt ctgtagtgcc taagtcgaga aacttaacta cggaagaagg ttgcggcaga
    58441 tgtgtcaaat ctttcatttt atcattaacg atggccgcta cgatgggcga agcataatgc
    58501 tctgccactt cctcgctgat ttctaacaag ctccgacctg cttctttagt tacgctggtt
    58561 tgatcaggta ggataatctt gatcgttgtc gtttgttcta tatctgtcac tgtagatccc
    58621 tctttcacaa gtattatcat tctgctgtcc tgagaatata cggcacaaca atggcgatgc
    58681 cttagtatct gaaaaaatga agtttaacta ggttaaacct gactccaagt tagagcacta
    58741 gctcgacaat atctttgtcc tgcaggaggt agtcacgggg aacggcttgg ccagcaaagc
    58801 gagctgaacc ccagacgagg gcgcttttga gcttttgggg aaaatcacga tgcacctgtg
    58861 cggctaaatc aagcactgta gaaccgcttt tcagaataaa gggacgctct cgatccgctt
    58921 ttttccccgg tgctttgcca taaatgcgaa tcacatccag cagcataaaa atattagctc
    58981 ttaaagctgt caaatcgtga tcaatagaga gaggttgtat ctgaatgtcg ggacgtaatt
    59041 cctgcaaaat ttccaggttg cttgtggcat ccggcaggtc agcctttaag ccaagcgtca
    59101 ggtagggcaa ggcaacaggg ctctcaccgc ttggatctat gatgcctcgc tcttccagca
    59161 gtgacagcgt agtttctgcc tgttccaggc aatcacctgt gctcaaatca attagcagta
    59221 gcagcgcatc ggcattgcgt aaagtaccga ttaagccggc agggagatta tcgttagtaa
    59281 acggcggggt gtccactagt tgaattaaaa tatcttcgta ctgcatcatg ccggctgcgg
    59341 gaattgtggt tgtataggga tagtccccga ctttaacttt tgctctgctt aaggccccca
    59401 ctaatgctga tttacctgta ttagggcaac ccgttaagac aacctgccct gcaccttgtt
    59461 tctccacatg aaaggggttg taagttcctt tttgctgctt tttttgttga ctttcctccc
    59521 gcatttgcga gagacgcttt tttaaatcag cttgtagttt ttctgtacct ttatgtttgg
    59581 gaatggtggc cagcatttct tgcagtgctg ctactttatc ttcaggcagg ctggcttttt
    59641 tatactgttc ttcagcggca tagtactgtg gggttaaatt tgcgggcaat tttatcacca
    59701 cactttccat gttctggtgt tattcttttt ctctttgggt cttggtgatt cctgctgagc
    59761 cttagttagt taattcacat cttgaatctc aggaatgcct gcgttactat gaaatagtat
    59821 atattaatgt ggattagact aataaataaa aatgggaggt tgacatgacg gcagcaagca
    59881 agaaatcgac ggtaaaagta ttagtgcctg ttttattact ggccttagtt tttggagtct
    59941 ggtatgtgaa aaatgctcaa tcagataaaa ttcccaccgg gggagttaac ccagattttg
    60001 ctttagcagt tacggaagag ttcgatctag aacacctaaa gtcatatgga ctgccaatta
    60061 tgcttgactt tggctctgat gattgtgcac cttgccaagc tatggctccc gtgttagagg
    60121 aattaaatct ggaattgcaa ggtaaagcca taattaaatt cattgatgtt tggaaatatg
    60181 aagagttagg gcaagattac cctcttactt taattcctac acaggtgttt atcgatgctg
    60241 aaggtaagcc ttacacgcca agcaatccga aagccatgga aatgacacta tttacgcatc
    60301 gtgagactaa tgagcatctg tttacaacgc atgttggacc aatgactaaa gaagaaatac
    60361 tggcagcttt agcggaaatg gggatgaaat aatggacagc agtcagtggc tgcagtcgtt
    60421 ggccacgatg attgctgcca agatgtggtt ggcaccactc tttgcttttg ctgctggtgt
    60481 gctcacctct tttaccccgt gtgcgctctc tagtattccc ttagtcatta gttatgttgg
    60541 cagcagtggc gaaggtgata caaaaaacgc tttttggctt tctgccatct ttgctctagg
    60601 gagtgctgtg acttttacaa ttttaggtgc tgctgcctcg cttttgggta gattaatgca
    60661 aagcgtcggt tcctggtggt atctggtttt aggggtgtta atgcttttaa tggcgctgca
    60721 aacatgggaa atttttagtt ttatcccctc ttcgtacgca gtaagcagaa ataagcaaag
    60781 gggttttggg ggggcctttg tcgctggtat tttagggggc tttttctctt caccttgttc
    60841 cacaccagtg ttagttgtct tactggcctt cgtggcacaa gaagggagtc tgctgtgggg
    60901 agtggttctc ttattactgt actccttagg tcatagtttg ttggtgctgc tggccggaac
    60961 atcgattgtt tttgtccaaa agttgtcccg cagcgataag tatggtaagt tcagtaatat
    61021 tctaagaatc ctcatgggta ccttaatgac cttgattgct ctgtatatgt tttatcttgg
    61081 tttctaattt attgaggtaa aggatagttg taaaaaatgg tttcccctgt tttctagtga
    61141 gacattatcg catgtgaatc actctcagat ccttaaccgc acaaaaaagt gttgaaaaaa
    61201 atagatttct atggtactat gacgaaagta taatattttt aagctggtac agagatgccg
    61261 acaagatatt aatgctaagg ttatagctag gagaatagcc ttatattgag gtataatctt
    61321 gtccgtgcgc tcgggcaagt tttttttgtg cccagatgga ggaagatagc aaactgtatt
    61381 gcttgggtat caaataatta gtaaggggat gttaagatgc aaaacaaaga attagtaaac
    61441 ggttacctcc cagatgagaa cccacctgtt tggcagctct tcttgtatgc tatacagcag
    61501 gttattgtga tgttccccgc cacagttgcg gtggcgatca ttacaggttt ccatatttca
    61561 acgacgattt ttgccagtgg actagcaaca attgcttttg tgctcgtcac cggcaggaaa
    61621 atcccacttt attatggttc gagtttctct tatgtggccg ctattggcag cctaatggcc
    61681 tctgagtctc ttgcaggata tcccctgaat gacaaaattg ctattgccca attcgggatt
    61741 atcatgtctg gtttcgtttc aattgctgca ggtattgttg taaatagatt tggccgggat
    61801 gccattgaaa agattctccc agcctccgtt actggcccta ttgccatggt tatcggctta
    61861 acgctggcag ctaccgctct acaggatgcg gcttctattg ctattgacac tgctggcacc
    61921 atcaatgtgg cagaagcaca aattgcgctt gccagtaatt ttgcctggac tatttcctta
    61981 gtaaccttac tttccacaat cttattctcg gtgtatctta agggagtttg gggacagctg
    62041 ccactgctgc ttggacctgt cctaggctgt atcactacct atatcattaa actggcgaca
    62101 ggtattagtc tctttaaggt tttaccggcg gcggcaaata ccagcatctt tgctttgccc
    62161 atgtttactt tgcccaaaat atcatggctg gcagtggctg ccattatgcc cattgctatt
    62221 gccacaattc ctgaatctac agcgcatgtt tatcaactag atatttatgt aaatgattta
    62281 gccaagaaaa agggatcaag caaaagatat aacattgcgg acaagctagg gcttaatttg
    62341 atcggtgatg gtattggtga cattgtttca ggcttaattg gcggtcccgc cggcaccaac
    62401 tatggtgaaa atattagtgc gatggcgatt actagggtct tctccgtacc agtgttaatt
    62461 ggggcttcaa taatcgcgat gattattgct tgcttcaccc cattgattaa tagtatttac
    62521 gctattccca cggcagttat tggcggcctg gaagtctatc tgttcggtgc catagcggca
    62581 caaggtatcg ctattatggt agaaaaaagt gttgatttat ttagctctaa aaatattgcc
    62641 atcattgcta caattatggt cattggtatt ggcgggcaat acggtttcgg cggcaacatt
    62701 ccattctttg gtctgcaggt gccgacaatt gccggcgctg ccatctttgg tattatcctc
    62761 aacttactgc tcagctttgg taaaaagaaa gatgcttaag tagaagcaga aaataaataa
    62821 atcagttttt cacaaaaagg aagccttggg cgatcaaggc ttctttgcat ctagactatt
    62881 gtcttggtgt gttactcgtt gacaaaaaaa gctgttaatg atacattcga atgaagattt
    62941 agaagggggc agaaccgtgg gagagaactt gacacgtaag attattggcg agcatttggc
    63001 tgcaggggaa atgcaggcag gaacagaaat tgccctgcgt attgagcaaa cattaacaca
    63061 agacgctacg ggtaccatgg cttacttaca atttgaagcc atggatgtag ctcgtgttaa
    63121 aacgcagctt tccgtctcct acattgacca taatacactg caaactggat ttgaaaatgc
    63181 ggatgatcac cgttatttgc aaacggtggc tgctaaatat ggtatttatt tttcacgtcc
    63241 aggtaacggt atttgccatc aagtgcatct ggaacgtttt ggtgtgccag gtaaaacact
    63301 actaggttca gatagccaca cgcctacagg tggcggactg ggtatgctcg ccattggcgc
    63361 gggtggttta gatgtggctg tggcgatggc cggcggtccc ttttatttaa ccatgcccaa
    63421 agtagtgcag gtgaaactag agggtcaatt acagccgtgg gtttcggcca aagatatcat
    63481 tttggccgta ctgcgctgtc tcagtgtcaa aggcggtgta ggtaaaatta ttgaatacac
    63541 tggtgatggg gtaaagactc tttctgtacc tgaacgggcg accattacca acatgggggc
    63601 agagttaggg gcgacaagct ctattttccc tagcgatgaa gtgacaagag cgttcctcgc
    63661 tgcccaaggg cgggaggagc aatgggtgga attgctgcct gatgccgatg cagcttatga
    63721 tgaaactttg accattgacc tatcacaact agaacccctg gtggcagctc cccatagtcc
    63781 cgatgctgtg aaaacagtac aagaactcgg tgagcttcct gtcgctcagg tggctattgg
    63841 cagctgcact aattcctcct atgctgacct aatgcaagta gctaaaataa ttaagggtaa
    63901 aaaggtgcat caagacgtca gtctggttat ttcgcccggt tcacgtcaag tgcttaccat
    63961 gctggcccaa aacggtgctc tcgcagacct aattactgcc ggtgcccgga ttttagaatc
    64021 aacctgcggc ccttgtattg gtatggggca ggcaccagca tctgatgcag tctctgtaag
    64081 aacgtttaac cgcaactttt tgggacgttc cggcactgcc agtgccagcg tatatttagc
    64141 tagcccggaa gtagcggcgg cgacagcttt aacaggtcga cttaccgatc cacgcaccct
    64201 ggggacttac tcgcccatta gccaaccgga gcaatttctg attgatgacg gcatgattat
    64261 tcctccggca gcgtcaggag aaggtgtgga agtagtacgc ggacccaata ttaagccgct
    64321 gcctgttaat agtccattac cgactgattt aactgccccg gtactcttaa aagtggcgga
    64381 caatattaca actgaccata tcatgccagc tggggctaaa gtactccctt tgcgctccaa
    64441 tataccagcg attagtgaat ttgtttttgc cgccattgac cttgatttta ccagccgcgc
    64501 taaagcagca ggcaagggta tggtaattgg cggccacaac tatgggcagg gctcaagtcg
    64561 tgagcatgcc gcactagccc caatgtatct aggggtaaaa attgtcctgg ccaaatcctt
    64621 tgctcgcatt cactgtgcta atttagtgaa cttcggtatt ttgccgctga ccttttgtga
    64681 ggaagctgac tatgaggccc ttaaacagga tgatatctta tcaattactg ccctgacaac
    64741 acagctgcag gcaggtaata ccatcaccgt gacaaatgac accagcggcg ttagcttcag
    64801 cgttaagcat gagctgacga accgccagat acaaattatc ctcgctggtg gtctgctaaa
    64861 ctttacgcga ggtcaggctt aagcgaatta aactaaaatt ctggcgcagc aagcgccaga
    64921 attatttttt ccgctttgat ttacatcaca gtttactctg ccaagctgtg ttatactttg
    64981 tttaaagcaa ttttgggagg tcaagacaga tgaaaagagc aattattcat attgatgaag
    65041 aaaaatgtaa tggctgtgga ttgtgcatac cttcttgtgc tgagggcgca ttgcaaattg
    65101 ttaatggcaa agccaaacta attgccgata acctttgtga cgggctgggc gcttgtcttg
    65161 gtgattgtcc ccaaggagcg ctaatcattg aagaacggga tagtgacgcc ttcgatgaag
    65221 aggctgttga acaattttta gcggcacaaa aagagcagac accgccaact tcaactccct
    65281 ttattggttg tcccggttcg cggacacgtg tcttaaatac gcccgcccca acggaaacag
    65341 cagtcgaggg agaagagatt ccttccgctt tagggcactg gcctgtgcag ttaaacttag
    65401 tcaatcccat tgccccctat tttaaagagg ctgatctgtt aatcgccgca gactgtgtgc
    65461 cttttgccta tgctgacttt catcgtcagt ttctgcgtgg ccatgcttta gtaattggtt
    65521 gtccaaaatt ggatgatgcg ccgctttatg tgcaaaagct aactgcattg attcgcagca
    65581 ataacctaaa aagtattacc attgtggtga tggaggtgcc gtgctgcggc ggactgcatg
    65641 gtattatccg tgaagcgatg cgacgtgccg agtgcaccgt ccccgttgaa gtcgtacagg
    65701 tttctgtaga cggcaagatt aattttcgct caaaatagca taagttgttt actagggggg
    65761 cgaacatagt tcgtcccttg ctcataggta tgactatggg tatcaagatt aggatatagt
    65821 aatgagtgta taaacccaaa aatcagtcac tggagggaca agcttgtcat ccattctaga
    65881 gaatactcat gactataccc ttattttgcc ttatgtcacg cgcatttttc cccttgtcga
    65941 cagggaacta aatgtatggt tagcaaaagc cagcaccatt ccggacccag aactgcgcaa
    66001 gcaagcttta gccagtattg ataaaaagcg attccactgc cagggtggta gtatttatgc
    66061 actttatacg cgagagaaga gtaccgagtt gatccacttt attgtggcat tacaaacgat
    66121 tagtgattac ttggataatt tatgtgatcg tgtgcctggc gctagtgaag agagttttcg
    66181 ccggcttcat caggcaattt tagccgcggt agatatcgag gaaacctgcc aagactggta
    66241 tgaacattat ccattctgca atgatggggg ctatttaaaa cagttagtag ctaggaccag
    66301 ggaaacacta gttcgtttgc ctggttacca ggatgtacgc acaacactgc gtagtttact
    66361 gcaattatat gtggatttac aggtgtacaa acatattgaa gagcccttac gtgtggaaag
    66421 gcttgtacac tggtattccc agaatcatag tttagcttcg gatgtctatt ggtgggaatt
    66481 ttccgccgct tccggttcaa ctttggctgt tttcctgcta acagctatgg cggctgcagg
    66541 gccggtcagc aaaagtgaaa tcgagcaggt tttagcctgc tactttcctt ggctgtgcgg
    66601 ctttcatatc cttttagatt acttcataga tttagatgaa gaccaacagc atcatgacct
    66661 gaactttgtc gactgttatc caacaccctt agtcgcagaa cggggactgt tgaacttttt
    66721 gtctgcagcg ctgcagaaaa cggccgcctt acaccgccca gcttttcatc gtactgtagt
    66781 ttacggtttg ttagcgctct atctttctga tcctaaagca aatgtgccgg aacggaaaaa
    66841 attatcgcag caattgctgc gaactggtgg caatgaggcg ctgtggctgc accgctgctg
    66901 tttgttatta cggaagctgg gtaagattta agaggaacgg ctcaccacat aatcggctaa
    66961 tcttgcgagc agcactcgtg ccggtttaag cggcagctgc gtaagatttt ctttggcctg
    67021 gaggatcagc gcccgggctt tttcgtatgt ctctttaata ataccgctgt cgtgcaagct
    67081 ctgacagata aaatctagat tttcaggaac aaaccgtttt tctgcaataa tttgctgggc
    67141 gcgccagcca tagtcaggat gctgcactag tttgattaaa ggtaaggtca gatatccttc
    67201 ccgtagatct tgtaagactg gtttgcccgt tgttttacta tcactcgtga tatcaagtaa
    67261 atcatcaatc agttgaaagg cttgccctag ctgcaaacca aacgtggcta gacactggct
    67321 gaattcctgc ggcaggccgc agacttctgc tcccgctgca cagcaggcag aaagaaagta
    67381 agctgtcttt ttataaatat aatcaaagta gtcgtcttct tgcagctggc agttgaagag
    67441 tctgctagac tgctcaattt ccccttcaca catcaaacta atggcgcgtg tcattttacc
    67501 taaaatactg gccaggccat gttcagttaa gatggagaag gcacgggcaa ataagaaatc
    67561 accataaagg acagcttgct gcgtgcccca ggtggcagag attgtggggc gcccccgccg
    67621 taaggaggcg tgatcaataa tatcgtcatg tacgagggag gcggtatgga ttaattctgc
    67681 cgccgttgcg gcatctacta acttgctttg tccgactggg taaaacgaac cacacaacaa
    67741 gactagtgct ggacgtaagc gtttgccgcc agtagtgaca gcggctgaga actggggtgt
    67801 ccagagatca gtgccgcggc ttaattcctg cagacgggct tcgaccagtt ttaagttggg
    67861 tagagcttgc aattccatta agggtgtttt ggctatggtc acatttatca cctattattg
    67921 taattacaat ttatcctcac ccattttctg ctgattattc tccagggtat aattgtcaac
    67981 cagtaatcat tcaaaaaaaa ttcaagattt tttcacattg atttggtata atgtaatttg
    68041 tatcgaaaga aggggaacca catttgcgct ccttgcttga ctgctaaaag tgccaaactc
    68101 ccctgcttta agctgtgagt cttaacagca tcagctacat gcttttattt ctgaactagc
    68161 caggtaaaaa agggaggtct ttttttgcaa aatataaaga acaataaacc attagtgaag
    68221 gtgattgtct tactgatctg tggggggtta gtcctttcct cggtggcagc agcgattatt
    68281 agtatcacag aaccgcagcc gactgaagat ccctcagcga tctatcgcag tcagctggcg
    68341 caagtagaaa ctgtaattgt acaaactgaa aagagtctgg aggaaaagcc agggaatgtt
    68401 gcgctcttaa aacagctggg gaacgcttac tatactgcgg gacaactcca ggcagcgcta
    68461 aatgaggcag aaaagtcgct ggagagtttt gcgcaggctt tagagcccta cggtaaagta
    68521 ttagagcagg aaccggataa tgttgaggtg cttgttgatc gtgctgtggc cgcctatgct
    68581 tcagaaaaca atgaaatagc gcaagaggat ttcgaaaaag ccatagctgt agctcctaat
    68641 catgccaaag ctcgttttaa ttatggtgtt ttcctttatt ttggcctggc tcagcccgaa
    68701 gaagcacttg ctcactggca gaaagtagta gaactaaatc ctgaagatga gcaagagctg
    68761 gttgccacga ccaaaacctg gattaaagcg gtagaagcag agttagcaga acaagccgca
    68821 gagctcgaag cagaaacaaa tgaagaagat tctgcgaaaa cagacgcaga gtaaaataat
    68881 aacctctgcg gttaacgtca gcaatgataa aaagaagctt tatgactggg aataaattcc
    68941 cagtcataaa gcttctttct ttaagtaaac aaactttaag taaacaaaaa aaatagcttc
    69001 tgacaatagg aacattttaa ctgtaaatag tggtatgtaa aggcagataa tttcagataa
    69061 taagacaagg acggaggtat ataatgacag ttgccacaca acttaaacaa acactagtct
    69121 ctttaaaagg ggcacaggct acactgcgtc tgtatgaaca acttagccga cagcaggaag
    69181 tgcgccatgc ctatcagcaa gcgctgcaga ctgtggaagc agtggttggt gatttggaag
    69241 taagaacaag ggaaattgaa agccaagaac cacagtatca aggctcataa agaggtgaaa
    69301 aaatgccagc ttggttaatg gtgttactgc gctcaagtgt actgtttgtt ttagttttct
    69361 ttttcattcg tgtgctggga accaaacatc ctgccaaaat gacacctttt cgttttgtca
    69421 actatgcggt aattgctgtg attatcggtt tactagcgtc tactgctatc gacagtttca
    69481 ttttaggtct ggcagcactc tcggtgtggt tagttatgcc ttttgtgctc gactatttag
    69541 aactacgtag taaagctgtt catgatctgc tggagggtaa ggaagcaata ataatcaaac
    69601 agggaaaaat catggaagaa aacctcaaac gtgctcgcct gactggggaa gaattattgc
    69661 gagatttacg cgctaaaaat atctttagta tctctgatgt ggaatttgcg gttttggaag
    69721 ctaatgggga gcttaatgtg atgctcaaat cagatcagca gccactgaca gcacatgatt
    69781 tggcatggca ggtgacgccg caaacggaac cgcaaactgt catcctggat ggcaatatcc
    69841 ttaatgatcc cctgactgag ctaggtctca atagtgactg gctgcaaacc gaattggtaa
    69901 aagcgggcgt ttctctggac aatgtctttc ttggtcaagt aaatagtgtt ggtgagctat
    69961 atatagattt gtttaacgat gccattaagg tcgagaatcc atctgtacgg caggccctgt
    70021 ttgctaccct ggagaaagca caagccgatt taacaagctt ttccctggaa acacagcagg
    70081 aacaggcaaa agcaatgttt gcccgcaatg ctgccgacct gcaaagtgtg ctggatggtt
    70141 tgcgaccgca ccttttacgc taaagaaggg agctaaaaat gtcaaataag aaaaataaaa
    70201 agttatcggc tgcacagcag gaatatcaaa aatttgctaa agatagagaa ccgaaacggc
    70261 cattgctaac taattgtctg catgccttta ttgctggcgg cctgatctgt acgcttggtc
    70321 aattctttca gtggttctat atgacttact ttaattttac cgaaatcact gccggcagcc
    70381 caacccaagc gacactaatc attatttctg ccctcttaac tagcttgggc gtctatgacc
    70441 atctggcaca atgggctggt gccggtacca tcgtgccggt aacaggtttt gccaatacga
    70501 tcacctctgc tgccattgaa caccgtagtg aaggttatgt cttgggcgta ggcggcaata
    70561 tgtttaaagt tgccggccct gtcattacct ttggcgtttt ttcagccttc gtggtggcca
    70621 tcatcaaaat tatcatccaa aagttaggtg gtatttaaaa tgctgcaggg tcatcagagt
    70681 tgggtatttg ataaacatcc ggtcatcatc agtactggtg tggcaggggg tccttttgag
    70741 gccgcgggag ctttagctga cgatttcgat attctgcatg atgacacatg gctaggacag
    70801 gagagttttg aaaaggccga gaaaaagttg ctggaagagg cctgtgaaaa ggctatcgat
    70861 aaagcaggta tgaaaaaaga agatgtgcac ttctttttaa gcggtgacct catgaatcaa
    70921 attatctcct ctagttttgc cgcgagaaca ttgggtattc cctatttagg cctgttcggt
    70981 gcctgctcca cctccatggc cggtctggca gtagcttcat ttattattaa tgctggtggc
    71041 gccagccatg tcttggctgc tgcttccagc cataacagtt ctgttaacaa acagtatcgc
    71101 tatcctactg aatatggcgc acaaaaccca cccaccgcac aatggacagt aacaggagca
    71161 ggagcagctt tagtggcagc agcaggtgaa ggaccgcggg tgacaggtgc gacaattggg
    71221 cgcattgtag atctgggtat ttctgatccc tttaatatgg gggcagcaat ggccccggca
    71281 gctgttgata ctattgaagc acatttccga gactttaatg ttgatgcctc ccattatgat
    71341 ttaattgcga cgggggattt aggaagagta ggtcataaca tagcgggcga tttactgata
    71401 aagcacgggg tagatatccc tgaggataag tttaccgact gcggtttact gatttataag
    71461 gataatcagc cagtaattgc gggcggcagc ggttgcgcct gttcagcgac agttacttat
    71521 ggtcaccttt taaatcgtat gcgccgcggc gagataaaaa gaattttggt ggttgcgact
    71581 ggtgctttgc tgtcaccatt atcatatcag caaaaggaaa gtatccccgg tattgcccat
    71641 gcggtagtaa tggagcggga aggagattaa gatggagaag tatctaattg cctttgtcat
    71701 tggcggtact atttgtgtca tcggtcaaat aatgcttgat gttttccgcc tgaccccagc
    71761 ccatatgaca gtcacactag ttgttaccgg tgctatttta ggtggcttgg gtctttatga
    71821 gccgttagtt aaattcgccg gtgccggtgc gtcagtgccc attagcagtt tcggtaattc
    71881 gctggtcaaa ggagcgttat ctgaagccaa aagcactgga cttgtggggg tgttaacagg
    71941 aatttttgaa gtaaccagtg ccggtatttc atctgctatt attttcgggt ttttggcctc
    72001 cttgtttttt aaaccaaaag gttagtgacc tagggagggg aaaggaggga gcagatgaca
    72061 gttggcacac aacttcagca ggctattgcc ggtatccaaa gtgctgcagc cagtatgaaa
    72121 acctttgcct tagaaacaca ggacacacag gctcaacaaa cttttaatca gttggcacaa
    72181 acactagata ctgctgtgga cacactcaag cagcgtcaac agtatgtcca aagccaagaa
    72241 ccacagtata aagaataaaa gcagaaagct ggaggcctcg ccgcctccag ctttttctgt
    72301 ttaagactaa attcgaacgc actcctgcag cattgtttta cgctaaccgt gctattttgt
    72361 cgtaggcagg gttaaattat tgacatatga ctaaaataac agtagaatga atatgggcat
    72421 atgccaataa tatagtaagg atttagtgca gacatattta attaacgagc gggaggttag
    72481 cgatgaaaat agcgatttta agcggcaaag ggggcacagg gaagacaacg gtagcgacta
    72541 gtcttgttgc ctcactacct gcatgccagt acatagattg tgatgtggaa gaaccaaatg
    72601 gttacatatt tcttaatcca gaagtgacca acaccattgc tgttaatgtt agggtgccta
    72661 aagttgatca agacaaatgt tcaggttgcg gcagctgtgc cgaagcttgt caattcaatg
    72721 ctttagctgt agtcaaggac aaggtgcttg tttttcctga aatctgtcat cactgtggtg
    72781 cctgtctgct tgcttgtcca gagggtgcta tctctgaaat agacagggaa attggtgtag
    72841 tagaagccaa tgcagataat acctttttac agggtagatt gaatataggt gaacccctta
    72901 gcatcccagt tattcagcag ttaaaaaaac acctgcatgg tgagttgccg ataattctcg
    72961 attgtccgcc tggtgcatct tgtgccgttg tacagtcaat cgaagactgt gattactgta
    73021 tcttggtggc agaacccacg ccgtttggtc tccatgattt acaaattgct gtgcggctgg
    73081 taaaactaat gcagattccc tgcggtgttg tgagcaataa agctacacca ggtaataata
    73141 cgatacaaga tttctgtgcg gaacatgagc tggagctcct tatggaaata cccttctcca
    73201 gagaaatagc tgcagcttac tcccggggta ttttgccagt aacgacctgc cctgagtggc
    73261 aggagaagtt ctgcgcatta taccagaaag tagagaaggg ggcacaaaaa tgaagcaaat
    73321 tgcctttgtc agtggtaaag gtggtacagg gaaaagcact ttggtttcct cactcagctt
    73381 acttgtaaca gataaaatgt tagcggactg tgatgtcgat gcccctaatc tgcacctttt
    73441 agtggcagga aatgtcatca aggaagatta ctttttcggg gcaaaggaag ctataattaa
    73501 tgcggaaaaa tgtcaacagt gcggcttatg ctctgagacc tgcaggtttg gtgccattag
    73561 tgacgaatta gaaattatac ctttaaaatg cgaaggttgt ggtgcctgtg ttttggtgtg
    73621 tcctcatgag gctatccgtt tggaagaagt gaaaactggc cgtacttacc tgacaaacac
    73681 gacccagggt acgttctcac atgcgctgct ggatattggt gccgaaggtt caggtagact
    73741 ggtaactgag gttaaaaaaa atatccttga gagctataag caggagaaat ggctgcttat
    73801 cgacggctct cccggcatcg gttgtgtagt tattgcctct atcacaggta gtgatgccgt
    73861 ggtcgccgtt acagaaccaa caaaatctgg cgtaagtgat ttagaaagag tgttgggtgt
    73921 agcgcaccac tttcagattc ctagcttcgt ctgtattaat aagtataatc ttaacctgga
    73981 gattacggcg cagctggaag agtactgcca actgcataat tgtccagtga ttggcaagat
    74041 accatttgat gagagtattg tcgcagctct gcaggcaggt aaaacacctg ttgaagctgg
    74101 gaacaataaa gttacccaag aaataataca attatggcag cggttaaccg cagctttggc
    74161 tgaagaagaa ccccttctta cctctatagg ggaagaaggg tgatagaaag gggattaatc
    74221 tgccttggta agacctacaa aatggcgtaa agttgagttt ataccagacc atcagctttt
    74281 ccgccccatg gatatagatg taaccctaga gaatgtttta caaattgaag aattagaagc
    74341 aattcgccta aaagacctag aaggattgga tcaagaggaa tgcgctaaaa gaatggaagt
    74401 atcgcggcct acttttcaac gtattctgag cgcagctaga ttaaaaattg ctgacagtct
    74461 aattaatgct aaagcaatta gaatcgcagg gggcaattat actcgtaata tttgtcccgt
    74521 taagtgtctt aactgcggca aggaatggga agaaagctat gagaactttg agaaagtctt
    74581 aagtggcaag tataactgcc ctgattgtgc ttcgacacag gttgcctgtg cacggcgtga
    74641 aaagaaaaag ttttgtcagg gtcactgcag aagacatggt ttacaagact gataaaaagc
    74701 taaaagggtt gagtgctgac tcaacccttt tagctttgta atgaggggaa aattataacg
    74761 ctgttatgtt agttgtcaca ccttagtggt cgcaggtatt gtcgcttagc ttcaaggtgc
    74821 tctgaagaaa gctcttaacc acttcctctg gcttgatggc gggagcacca gtcaaaactt
    74881 gtacacctgc ggactgaaaa agctgttgtg catggccacc catgccgcct gttataacca
    74941 gctcagcacc ttgttcccta acccacacgg gaaaaaggcc tggttcatga ggtggtggca
    75001 ccaacaattt taccccttta atagtttttt ctgtagtgtc tacatcaaaa aaagcaaact
    75061 tctcacagtg gccaaaatgc agacaaagct ggtcttctac catcggtaca gcaataatca
    75121 tatgtatctc tccctcaata gtgtttttat ttatacccaa tgatcttcta catcgttagt
    75181 ttcagctgtt tctagagtac cctctttaaa gctggtaata gcctcttgta cagtaatttt
    75241 ccgtgctaaa tgaacggaaa catcagtaga ttttaatact ctaaatgctt taggtcccac
    75301 atgcccagta attatcgcct ccgctccagc attgatcaca ttttgtgcag cctgaatacc
    75361 tgctccctgt ggggcattgt aaacttgttg gttgtctaat aacttatacg tctcatcttc
    75421 taaatcataa ataataaatc ttgctgctct accaaagcgt tcatccatgg gatcatccaa
    75481 tgttgttcct tgtacactga cagcgacttt catatttacc ctccacccaa gtagtaatta
    75541 gcatatgccc acaatcaata taccatagta ttgagcatat gtctataact tattcgtaaa
    75601 tttttgccaa agttaataac ttgcgttaga tctctttccg cagacataca acatcttaca
    75661 ttcccttagt cgctgcttca cacagaattg tttgccgccg gagaaaaaag gtttgatcat
    75721 gggattaagt atgaaaagtc gatggtaagt atcaacttcc gtcgactatt actatttaat
    75781 gggtagcttc cttctcgatg gtaggcttaa actgtataag ttaggtccat agatcaaaca
    75841 agtccagctt gaatcctaca taaaaacgta attatttaaa attaaaaata taatgcttgt
    75901 gttttcaaaa tagatgcgat ag
//
LOCUS       DASRMS010000205         3472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_996838, whole genome shotgun sequence.
ACCESSION   DASRMS010000205 DASRMS010000000
VERSION     DASRMS010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3472
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996838"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            148..558
                     /gene="queF"
                     /locus_tag="VFC74_09915"
     CDS             148..558
                     /gene="queF"
                     /locus_tag="VFC74_09915"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HZK25680.1"
                     /translation="MSQSDFYALGKVVKEPTRKLDVFPKPAGVQTIVMESDEVTSLCP
                     ITGQPDWETVIIEYLPDQLCIESKSLKLYFWSFRQEGAFCEALAAKIANDVGAACQPH
                     SCQVTVIQKPRGGITIRATACYEKGKVAEVKQES"
     gene            563..1225
                     /locus_tag="VFC74_09920"
     CDS             563..1225
                     /locus_tag="VFC74_09920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HZK25681.1"
                     /translation="MTSTLSRQAKVFLLLSCCFTVLLVVSNIIAGKIIIVGTLMAPAA
                     VICYSLTFAITDTLAEIWGKERTQFVVNLGFLTVILSAVFIRLALVLPSAPLWHGQEV
                     FAAVLGSNLRMVAASLTAYVISQYHDVWAFTFWKEKTNGRHLWLRNNLSTTVSQLLDT
                     VVFITLAFYGTGVSLWSMIAGQYLVKVIIAFCDTPLVYALVYLIKKYAKFEELNSPAK
                     QT"
     gene            1354..1587
                     /locus_tag="VFC74_09925"
     CDS             1354..1587
                     /locus_tag="VFC74_09925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25682.1"
                     /translation="MTIIRMEMGPRIDARDLENLRNHLANLDRGDEITIRVEAHDTEL
                     IASALTEHGFNWQPHGSHSGQDYFLTGKKIKRH"
     gene            1850..2176
                     /locus_tag="VFC74_09930"
     CDS             1850..2176
                     /locus_tag="VFC74_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25683.1"
                     /translation="MESYFYDQDLAMVYKISPVAATVVEDEDKKVPTAILVHANVKVT
                     NFKREKVRRTISEVYPYDQYDFDTAKEAFTNNVLQKVLGNATAISEEEYNVIKQRVEP
                     ASHCTN"
     gene            2325..>3472
                     /locus_tag="VFC74_09935"
     CDS             2325..>3472
                     /locus_tag="VFC74_09935"
                     /inference="COORDINATES: protein motif:HMM:NF012308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serpin family protein"
                     /protein_id="HZK25684.1"
                     /translation="MRQTVVILLCLLLAMPLVGCSKGTIPGKKAEAAERVDSRLVAGN
                     TDFAFRLFTQLRQPAGDTNTFFSPASIAFALGMVYNGAAGETQAEMAQVMGVASLTLE
                     ELNKANADLLTILQNPDAKVETSIANSLWPDDDARLKADFMQRNRDFYAAEVETLDYA
                     QAGAHKTINSWVEKQTNGKIKDLFDRLEGDTRLVLVNALYFNGEWTEAFDEELTQEAP
                     FTLNQGTQKDVPLMHKKAAFECLEDDSFTAARLPYGKERLSMYVFVPKKDLTDFYQQL
                     NPDSWQKWLSLFAEEEVSVYLPRFKAEYKQQLNSALQEIGMKQAFIPDLADFRAMAEE
                     ELFISDVIHQAYLEVHEKGTEAAAATGVVMPTESLPAEPFTVRADRPF"
BASE COUNT         1021 a          682 c          776 g          993 t
ORIGIN      
        1 atacatatcc ccgagttagc aatacataaa taaaaaataa actgcatatt taattaatgc
       61 agcttgtcga aatgtcgaat tcttgataaa gttagatttg tggcggtcga agtaacccgc
      121 cttatcaaaa caaaggaggt aattccattg agtcagtcgg atttttatgc tttgggtaaa
      181 gtggtaaaag agcctacccg caaattagat gtcttcccca aaccagcagg tgtgcaaaca
      241 atagtgatgg aaagtgacga ggtgacaagt ctctgtccca ttactggtca gccggattgg
      301 gaaactgtaa ttattgagta tctacctgac caactgtgca ttgaaagcaa aagtttaaaa
      361 ctttactttt ggagttttcg tcaagaaggc gctttctgtg aagctttggc agcgaaaatt
      421 gctaacgatg tgggtgcggc ctgccaaccg catagttgtc aagtaaccgt gatccaaaag
      481 ccgcgcggcg gcattactat tcgtgctact gcgtgctacg agaaaggtaa agttgctgaa
      541 gtcaagcagg agtcttaatt agatgacaag cacactctca cgtcaagcta aagtttttct
      601 cttgttaagt tgttgtttta ccgtcttatt agtcgttagt aacataattg ctggtaaaat
      661 tattattgtt ggtactttaa tggcaccagc agctgtaatt tgttactctt taacctttgc
      721 cattacggat accctcgcag aaatttgggg caaagaacgc acgcaatttg ttgtcaattt
      781 gggttttctg acggtcatct tgtcagcagt ttttattcgc ctagccctgg tgctgccatc
      841 cgctccgctt tggcacgggc aggaagtctt tgctgccgta cttggtagta atttacgtat
      901 ggtcgcagct agcctcaccg cttatgtcat cagtcagtac catgatgttt gggcctttac
      961 cttttggaaa gaaaagacta atggtcgaca cttatggctg cgtaacaacc tatcaacaac
     1021 tgtttcgcaa cttttagata cagtcgtttt tattacgcta gccttttatg gcacaggagt
     1081 atcattatgg tctatgattg ctgggcagta tctagttaaa gtgatcatcg ccttttgtga
     1141 tacacccctg gtttatgcgc tggtctacct aatcaaaaaa tatgcgaagt tcgaagaatt
     1201 aaattcccca gcgaaacaaa cttaagcaaa aagcggccaa atggccgcct tttgctttta
     1261 aaattgctat tttctatcaa agtagtgttt atttttttga gacggggaaa atttagatta
     1321 agataatctt ttgcgagcga aaggagtgtt ttagtgacga ttattcggat ggagatggga
     1381 cccaggattg atgcccgcga tttagaaaac ttacgcaatc atttggctaa tctagaccgt
     1441 ggagatgaaa ttacaattcg tgttgaggcc catgatacag agctaattgc ctcggcacta
     1501 acagaacatg gttttaactg gcagccgcat ggcagccata gcggacagga ttatttttta
     1561 actggtaaaa agatcaaacg ccattgattt aactaacaga gccttccctt taataggtga
     1621 gggctctgtt aagtttaatc acaatctgga atatcttaaa gataaccagc aatagactat
     1681 agttgaatga agattacaaa agcattaacc ctgctattgc tggcaggaaa aatcagtttt
     1741 gtggcgaatt ttgtattaag taaggagatg tattatttcc agggttgggg aaaatattta
     1801 caaccatggg aagcaatctc aaactgtaag caaaaaggag ggctatacaa tggaaagtta
     1861 tttttatgac caagatctgg ccatggtgta taagattagt cccgttgcgg ctaccgtagt
     1921 agaagacgag gataaaaagg tccctaccgc tattctggtg catgcgaacg tcaaagtgac
     1981 taattttaaa cgggaaaaag tacgccgtac catatctgaa gtgtatccct acgaccaata
     2041 tgatttcgat actgcaaaag aagctttcac taataatgtg ctgcaaaaag tgttaggtaa
     2101 tgctactgcg attagtgaag aagaatataa tgtcataaaa cagcgcgtgg agccggcttc
     2161 tcattgtact aactaaagct gaatagtaac ctctaaacag acttgtcctt tgtgcaagcc
     2221 tgtttttctt tgtagctagg aaatcttttg ccgctgcaac gtggaacttt taagtattat
     2281 gctcgtctat acttagataa tagtgtaaac aaggaggcct accgatgaga caaacagtgg
     2341 ttattttgct ttgcttactg cttgctatgc cacttgttgg ctgtagtaag ggtacaatcc
     2401 cggggaagaa agctgaagca gctgaacgcg tagattcgcg tctggtagca ggcaataccg
     2461 attttgcttt ccgtcttttt actcaactac gccaacctgc aggtgatact aacaccttct
     2521 tttctccagc cagtatcgcc tttgcgttgg gcatggtcta taacggagca gccggtgaaa
     2581 cacaggcaga aatggcgcaa gtgatgggtg tcgcctcact caccttggag gaactcaata
     2641 aagctaatgc agatctctta acaattctgc aaaaccccga tgccaaggta gaaacaagta
     2701 ttgctaattc cctctggcca gacgatgatg ctcgccttaa agcagacttc atgcaaagaa
     2761 accgcgactt ttatgctgct gaagtagaaa ctcttgacta tgcacaagct ggtgcgcata
     2821 aaacaatcaa cagctgggtt gaaaagcaga ctaatggtaa aattaaagat ctttttgatc
     2881 gcctagaagg ggatacacga ctggtactgg ttaacgccct ttattttaac ggagagtgga
     2941 cagaagcctt tgacgaggaa ttaacgcagg aagcaccttt tactcttaat caggggacgc
     3001 aaaaggatgt acctctaatg cataaaaaag ctgcttttga atgtctggaa gatgactctt
     3061 ttactgctgc acgcctgcct tatggcaaag aacggttaag tatgtatgtt tttgttccca
     3121 aaaaggattt aactgacttt tatcagcagc ttaacccaga cagttggcaa aaatggttaa
     3181 gtctctttgc cgaagaagaa gtaagtgttt atttgcctcg ctttaaagcg gaatataagc
     3241 agcaattaaa cagtgcccta caggaaatag gcatgaagca agcatttatt cctgatttag
     3301 ctgatttcag ggccatggca gaggaagaat tatttattag tgatgttatc catcaagcct
     3361 atcttgaagt gcacgaaaaa ggaacggagg cagctgctgc cactggagta gtaatgccca
     3421 cagaaagttt accggcagag ccctttactg tacgtgctga ccgccccttc tt
//
LOCUS       DASRMS010000206         9581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_660859, whole genome shotgun sequence.
ACCESSION   DASRMS010000206 DASRMS010000000
VERSION     DASRMS010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_660859"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..612)
                     /locus_tag="VFC74_09940"
     CDS             complement(250..612)
                     /locus_tag="VFC74_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HZK25685.1"
                     /translation="MSQKILNILSGEPGEGMKELLFNQGDVKAVRVILEAGQVSADCI
                     VDAPVLLFVISGEGSVIIDDQTFALSEGDVTVVPSGINRHLAASDRQFSLLAVQSHQA
                     EKSCGLCALLESCVNLKD"
     gene            complement(593..1165)
                     /locus_tag="VFC74_09945"
     CDS             complement(593..1165)
                     /locus_tag="VFC74_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014902361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HZK25686.1"
                     /translation="MKKLATQQAILAAAKELFIANGYKGTTTVQIAKQAGISEMTLFR
                     HFAKKEDIFLQVIAPVISFLDQLAVAKQQNIQQTVREMLQNRLLFLLEEKELVRLVVL
                     ESYVGEGQSNPIVEVVQKLEEHFTALQTDERELLIRLIAGFILSGIFLPKNKSSDMQM
                     MESLLQSVIYPLLDSFTHGQEEMKNVTKDS"
     gene            complement(1268..2422)
                     /locus_tag="VFC74_09950"
     CDS             complement(1268..2422)
                     /locus_tag="VFC74_09950"
                     /inference="COORDINATES: protein motif:HMM:NF024839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HZK25687.1"
                     /translation="MLCLIICCTGKVVSAATADSSCRECHSGAAADLDRGSKGEFMLC
                     TDCHGSDHNGPGTGEPGTPTPKTCAQCHAEEVAQFNEGKHYWAIDAMEAVPTYQKMPS
                     EVTDKGCVVCHQVGQKWDDGSQGRCDSCHTRHVFSAAEASTPESCGTCHTGDHPQYEI
                     WANSKHGKLYNMEGGEERAPACLDCHDSHRVVTAWGFLGLRPGEQEDEEWLTARNKVQ
                     QALETMGPGLAPNVTRETYVEWEGLREEMVTRCTECHAESYVREDLAKSDALVREADL
                     MMANVVTVADMLYDQNLVDEQSRFMIYRDSTAHRFATFMGGFHNSALYSWDKGYLGLT
                     SDMVALRDRAITAKKASLVQTAVIAAVVLIFLLIITTIYLLRKRKQAEQK"
     gene            complement(2524..2769)
                     /locus_tag="VFC74_09955"
     CDS             complement(2524..2769)
                     /locus_tag="VFC74_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25688.1"
                     /translation="MKRTTFIILAVVMMALGGIYLRAGVERTEPPQATGPASPASHDL
                     DDTHADCLSCHAVIKSSHDQRFGEGNYSDCLSCHAQK"
     gene            complement(2868..4871)
                     /gene="ligA"
                     /locus_tag="VFC74_09960"
     CDS             complement(2868..4871)
                     /gene="ligA"
                     /locus_tag="VFC74_09960"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HZK25689.1"
                     /translation="MDREKALAVIEQLKQEITEHNYRYHVLDEPTISDTDFDSLVRQL
                     LAIEEQYPELVTSDSPTQRVGGQPRAGFVQVEHRLPLLSLDNALNLGDLRDFVRRATN
                     LVPEADMEFVVELKVDGLAVSLQYENGVLVRGATRGNGEVGEDITHNLRTVKSIPLRL
                     RQPLTLEARGEVYMPRSSFLALNEERAAQDQPLFANPRNAAAGSLRQLDPKVAAARNL
                     AMVTYSLGYSPHLHPSTHYEALMSLQKLGLRVNPKLKILHDLEAVVEYCESWREKRYD
                     LPYDIDGLVVKINNLALQEQLGNTAKSPRWAIAYKFPAEQAVTQVLGITVQVGRIGTL
                     TPIAELAPVKVAGTIVKRASLHNEDILREKDVRIGDEVIIQKAGEIIPEVVEVVKTKR
                     SGEEQPFQMPTVCPACGSPVNRLPGEVALRCFNLACPAQVLERIVHFASRGAMDIEGL
                     GPAMAEQLLAAELIQDAADIYSLHEKREALLQLERKAKKSVANLLTAIEKSKKQPLWR
                     LLYGLGIRFVGDRTAKLLAEHFASLDVIMAVSREELEAVAEIGPKIAESICDFFSLDS
                     SQTLVARLRHAGLNFVQEKEKIGNLPLAEKVFVLTGTLPTYSRDEAAQLIEAAGGKVT
                     GSVSKNTDYLVAGDKAGSKLDKAKQLEITILDETGLQQLLPER"
     gene            complement(4882..7005)
                     /gene="pcrA"
                     /locus_tag="VFC74_09965"
     CDS             complement(4882..7005)
                     /gene="pcrA"
                     /locus_tag="VFC74_09965"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HZK25690.1"
                     /translation="MTQINDGLNEMQRQAVECVEGPLLVLAGAGSGKTRVITHRIAYL
                     IKQELAKPWEILALTFTNKAAEEMRTRVAQLVGTIPGMWVSTFHAAAVRMIRANAALL
                     GLTSSFVIYDSMDQMTLVRNIMKDLNLDSTQFKPRGLLAAISRAKNDLLDEHRYEDTA
                     DDYYTRTVARIYKSYQERLRLNNVLDFDDLLLYVVKLLQEQPAVLSSYQQKFRYILVD
                     EYQDTNRVQYEIVLLLSGEHRNLCVVGDDDQSIYQFRGADIRNILDFERDWPEATVVK
                     LEENYRSSGNILEAAYHVVKNNAGRKAKKLWTSRDKGEAVILQVAEDEHQEARYITAE
                     IRKLSSQYEQFAILYRTNAQSRALEEALMRENIAYQMIGGVRFWERKEVKDILAYLRV
                     LDNPADDISLLRIINVPRRGIGNTTIERLRALAAEKHCPISEILPTAQEAGLTPGPLK
                     KVQAFTTLMENLTKMGAYLSVDELAEQVMTKSGYLDVLRAERTEQAQSREENLREFLT
                     VAQEFQRTSEDQTLTTFLTQLALISDLDTLSDEDVPRVVLMTLHAAKGLEFPVVFMAG
                     MEEGLFPHKRSFETADGIEEERRLCYVGITRAQQLLYLTHARRRNIFGAIMAGLPSRF
                     LEEIPPSVLQEQAETPQPAAAAPAPGEVGDLAVGDSVEHGKWGQGEVRSVDTLSDGDI
                     VLTIHFPSIGLKKVIARYAPLRKVK"
     gene            complement(7443..7739)
                     /locus_tag="VFC74_09970"
     CDS             complement(7443..7739)
                     /locus_tag="VFC74_09970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HZK25691.1"
                     /translation="MASSKFKDKNTDLLFEAILLLENEEECYQFFEDICTINEIKAMA
                     QRLHVAKMLQEGNTYQQIEQETGASTATISRIKRYLNYGAGGYKLLLERLKTNT"
     gene            7898..8683
                     /locus_tag="VFC74_09975"
     CDS             7898..8683
                     /locus_tag="VFC74_09975"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HZK25692.1"
                     /translation="MIRKLTMEDHQQVENYLQVLPSFSLFLVADIEKYGYDSSVFTVW
                     GIFSHTGDLLHVFGKYYHTLVVYSNTAAYPAAEVNAFLSDQAIEYDTILGRSVLVEPF
                     LADLSFAQIFRNYYCELNRENFTPLINQAINVTKADLSQTKAILNLLASIKEFPRASF
                     QEDALNTYIADDGVYLIMEDDEIASLAMCSARTAGLANIGGVCTAKPYRRKGYASTVV
                     SALSKKYLEQGLICCLGYNNPEAGAIYQRLGYQTVGHIIIATK"
     gene            8915..9298
                     /locus_tag="VFC74_09980"
     CDS             8915..9298
                     /locus_tag="VFC74_09980"
                     /EC_number="1.-.-.-"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009614713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HZK25693.1"
                     /translation="MKKINDQLKEILSHPADGAVAIVTQGKDGPHVVNTWNSYIKQSA
                     DGRWLAPASRMNKTEENLAADNRVKLTVSSREVQGKMYKGTGFLITGTAEFLKDGPDF
                     ETIKGKFDWARAALAITIETAEQTL"
     gene            complement(9355..>9581)
                     /locus_tag="VFC74_09985"
     CDS             complement(9355..>9581)
                     /locus_tag="VFC74_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25694.1"
                     /translation="KEEDIGKFCAFVLTYDPAFTFWKYQFILAVKETLVKKLKLPWSA
                     VFLEMEKELQRMSERRQRHDFIPARLKAIK"
BASE COUNT         2553 a         2329 c         1978 g         2721 t
ORIGIN      
        1 aaatagttgt gtcccataca tatttatgca aaaacacgaa ggccgcgaac cctttattta
       61 caggggttgc ggcctttatt tcgcattttt tgctgtcaaa gtcaggatta taccataacc
      121 accactgctc ggcaacttta acgacgttcc gccctctcca aaaacaaaaa aaagtaccac
      181 acttgggtac ctcttttaat gaataggcat tgttaaagca aattagctaa actgtgacca
      241 tcaagcgttt taatctttta ggtttacgca gctttcaagt aatgcacaga gaccgcagct
      301 tttttcggct tggtggcttt gcactgccaa aagagaaaat tgacggtcgc ttgccgctaa
      361 atgtctgttt atccctgacg gcaccacggt aacatcacct tcgctgaggg caaaggtctg
      421 atcatcaata atcactgaac cctctccgga aatgacaaac aataagacag gcgcatccac
      481 gatacagtct gctgacactt gcccagcttc caggattaca cgtacggctt taacgtcgcc
      541 ttggttaaaa agcagctctt tcatcccttc accgggttca ccagatagga tattaagaat
      601 cttttgtgac atttttcatc tcctcctgcc catgagtaaa gctatcaagt agtggataga
      661 taacggattg cagcaagctc tccatcattt gcatatcaga acttttgttt ttgggtagaa
      721 agataccgga cagaataaag ccggcaatca gacgaattaa taactcacgt tcatctgtct
      781 gcaaagcagt aaaatgttct tctagctttt gcacaacttc aacaatcgga ttgctctggc
      841 cctcgccaac gtaactttcc agcaccacta gacgaacaag ctctttttcc tctaacagaa
      901 acagtaaacg gttttgtaac atttctctca ctgtctgctg tatgttttgc tgtttggcaa
      961 cagctaactg gtctaaaaaa gaaattacag gggctatgac ttggaggaaa atatcttcct
     1021 ttttagcaaa atgacggaaa agagtcattt ctgaaatacc tgcctgtttg gcaatctgca
     1081 cagtcgtagt acccttataa ccgttagcaa taaataattc tttagcagcg gctagaatag
     1141 cctgttgcgt tgctaatttc ttcatttgat acccccagca ggttactttc ttcaagctta
     1201 ctgcttaaac acgctaactg tcaagagaga gtaacatccc ctcttgacag ctagaggatg
     1261 cggtagatca cttttgctct gcttgcttac gttttcttag taaatagatg gtggtgatga
     1321 ttaagagaaa aataagcact acagctgcaa tcacagctgt ttgcacaagt gaagcttttt
     1381 tagcggttat cgctctatct cgcaacgcta ccatatctga ggttaggcct aaatagccct
     1441 tatcccagga gtataaagct gaattatgaa aaccacccat aaaagtagca aaacgatggg
     1501 cggtgctatc gcgataaatc ataaaccgtg attgctcatc gactaaattc tgatcgtaca
     1561 gcatatcggc aacagtaacc acgttagcca tcattaaatc agcttccctt accagagcat
     1621 cacttttagc caagtcttcc ctgacatagc tttcagcatg acactcggtg cagcgggtaa
     1681 ccatttcttc ccgcaaacct tcccactcaa cataggtttc ccgcgtaaca tttggtgcca
     1741 atcccgggcc catggtttcc aatgcttgct gtactttatt cctggctgtc aaccactctt
     1801 catcttcttg ctcgcctgga cgcagcccca aaaatcccca cgctgtcaca acacggtggc
     1861 tgtcatggca gtctaaacaa gccggagccc gttcttcccc gccctccatg ttgtacaatt
     1921 taccatgctt tgagttagcc cagatctcat actgggggtg atcccctgta tgacatgtac
     1981 cacaagactc tggggtacta gcttcggctg ccgagaatac atgacgcgta tgacatgagt
     2041 cacagcgacc ttggctgcca tcatcccatt tttgaccaac ttgatgacat actacgcagc
     2101 ccttatcagt tacttcactt ggcatcttct ggtatgtagg tacagcctcc atagcatcaa
     2161 ttgcccagta atgcttgcct tcattaaact gtgccacctc ttctgcatgg cattgtgcgc
     2221 aagtctttgg tgttggtgtg ccgggctctc ctgtaccggg gccattatga tctgagccat
     2281 ggcaatctgt acataacata aactcccctt tgctgccacg atccagatct gcagccgcac
     2341 cactgtggca ttcacggcag ctgctgtctg ctgtcgctgc cgacacgact tttcccgtac
     2401 agcagatgat cagacagagc aataggcatc ctccccatcg tcgcctcaca gctaatccct
     2461 ccttaaactg ttagcattca ctaacattat cagctgggag gttaattttg tcaagtactt
     2521 ttgctatttc tgggcatggc aactcaaaca atcactataa ttaccttcac caaaacgctg
     2581 gtcatgggag cttttaatta cagcgtgaca gcttaaacaa tctgcatgcg tatcatcaag
     2641 atcatgacta gctggtgatg ccgggccagt tgcctggggt ggctctgtgc gctccacacc
     2701 tgctctgaga tagatgccgc ccaaggccat cattacaaca gctaaaatga taaaagttgt
     2761 ccgcttcatt ggctcctcca caagtttttc cttagtatac ccgaggcaga gaagactgcg
     2821 cagacaaaaa agaaactccc tatttttaag ggagtgaaaa tttatattta gcgttctggt
     2881 aatagttgct gtaaaccagt ttcatctaga atggtgatct ctagttgttt agctttgtct
     2941 agtttactgc cagccttgtc acctgccacg agatagtcgg tgtttttact gacactgcct
     3001 gttactttac cgcctgctgc ctcgattaac tgcgcagctt catcacggct gtaagtaggt
     3061 agggttcctg tcaaaacgaa aaccttctca gctaaaggta aattgcctat cttttctttt
     3121 tcctgtacaa aattaagacc agcatgtcgt aggcgggcca ctaatgtttg tgaggagtca
     3181 agactaaaaa agtcacagat actttctgca attttagggc ctatttccgc taccgcttct
     3241 aactcttcac gactaacagc catgatcaca tctaaagaag cgaaatgttc agccaagagt
     3301 tttgctgtgc ggtcgcctac aaaacgaatg cctagcccat ataacagacg ccataaaggt
     3361 tgttttttac ttttctcaat cgccgttagc aaattagcta ccgatttttt tgctttacgc
     3421 tccagttgca gcagcgcctc tcttttttca tgcagagaat aaatatctgc cgcatcttga
     3481 attaattcag ccgctagcag ctgttctgcc atcgcggggc ccagcccttc aatatccata
     3541 gcaccacggg aagcaaagtg cacaatccgc tccagcactt gagctgggca ggcaaggtta
     3601 aaacaacgca gggccacctc accaggcaga cggttaacag gactgccaca agccggacat
     3661 actgtcggca tctgaaacgg ttgttcttcc ccgctgcgtt ttgtttttac aacttccacc
     3721 acttccggta taatctcgcc tgctttttgg ataatgacct catcgccaat acggacatct
     3781 ttctcccgta aaatatcttc attatgcagg ctggcacgtt taacaatcgt acctgccacc
     3841 tttacggggg ctaattccgc tatcggcgtc aaggtgccaa tgcggccaac ctgtaccgta
     3901 atccctaaaa cttgcgttac ggcttgttca gcaggaaatt tgtaagcaat cgcccaccgc
     3961 ggacttttcg ctgtattgcc aagctgctcc tgcagcgcta gattgttaat cttgacaact
     4021 aaaccatcta tatcataagg caggtcatag cgtttttccc gccaactttc acaatactcc
     4081 accaccgctt caagatcatg caagatctta agcttggggt taacacgtaa gcctaacttt
     4141 tgcaaagaca ttaaagcttc gtagtgcgtc gacggatgca gatgcggaga atagcccaag
     4201 ctgtaagtaa ccattgccaa attacgcgct gcggccactt tggggtctaa ctgccgtaag
     4261 ctgccggcag cagcattgcg gggattggcg aataagggct gatcttgtgc ggcccgctct
     4321 tcattgagag ccaaaaacga ggagcggggc atatacactt cgccgcgagc ttctagggtt
     4381 aagggctgac gcaaacgcag cggtatgctt ttgacagtgc gtaaattgtg ggtaatatct
     4441 tcaccaactt cgccattacc acgggtagca ccccttacta acacgccatt ctcgtactgc
     4501 aaggacacag ctaaaccatc tacttttaat tctacgacaa attccatatc ggcttccggc
     4561 actaaattcg tggcgcggcg gacaaaatca cgcagatcac caagattaag ggcattgtcc
     4621 agggaaagca aaggtaagcg atgctctact tgtacaaaac cagcgcgagg ctgtccacca
     4681 acacgctgtg tgggggagtc ggatgtaacc aattcaggat actgctcttc tattgccaac
     4741 agctgacgca ctaaactatc aaaatcagta tcagaaattg tcggctcgtc gagtacatga
     4801 taacgatagt tatgttctgt aatttcttgc tttaactgtt ctattactgc cagggctttt
     4861 tccctgtcca tcttcggcac ctcactttac tttacgcaaa ggcgcataac gagcgataac
     4921 ttttttcaag ccgatactgg ggaaatgaat tgtgaggaca atatctccgt cactaagggt
     4981 atcaacactt cttacttctc cctgccccca tttaccgtgt tcaacactgt cacctacagc
     5041 taaatccccc acctcaccgg gcgctggagc agctgccgcc ggctgtggag tctctgcttg
     5101 ctcttgtagt acagaagggg gaatttcctc gagaaagcgt gaaggcaggc cggccattat
     5161 ggcgccaaag atgttgcgac gacgagcatg tgttaaataa agaagctgct gggcccgagt
     5221 aatacctaca taacacaaac gccgttcttc ctcaatacca tcggctgttt caaaagaacg
     5281 tttgtgggga aacaatcctt cctccatacc ggccataaaa acaactggga attccagacc
     5341 tttggcagca tgcaaggtca tcaagacgac ccggggtaca tcttcatcac ttaatgtatc
     5401 caaatcacta atcaaagcta gctgtgtaag aaaagtagtt agtgtttggt cttcactagt
     5461 acgttgaaat tcttgggcta cagtgagaaa ttcacgcagg ttctcttctc ggctttgtgc
     5521 ctgctctgtt ctttcggcac ggaggacatc taaatagccg cttttggtca tcacctgttc
     5581 tgccagttcg tctacactta agtatgcccc cattttagtt aaattttcca taagtgtagt
     5641 aaatgcttgt acttttttta acggaccagg agttaacccc gcttcttgcg ccgttggcag
     5701 aatttctgaa ataggacaat gcttttctgc agctaaggct cgtaagcgtt caatcgtcgt
     5761 attgccaatg ccgcggcgag gtacatttat aattcgtagt aaactgatat catcagcagg
     5821 gttgtctagg acccgtagat aggccaaaat atcttttact tctttgcgct cccagaaacg
     5881 cactccgcct atcatctgat aagcgatgtt ttctcgcatt agtgcttctt ctagagctct
     5941 ggactgggca ttagtgcgat aaagaatggc aaactgttca tactgcgagg aaagtttgcg
     6001 aatttctgcc gtgatatagc gtgcttcctg gtgttcatct tccgcaacct gcaagatcac
     6061 cgcttcaccc ttatctctgc ttgtccataa tttttttgct tttcgacctg cattgttttt
     6121 taccacatga taagctgctt ctaaaatatt gccactggaa cgatagtttt cctccagctt
     6181 taccacagtg gcctcaggcc aatcccgttc aaagtctaga atgttgcgta tatcagcacc
     6241 acggaactgg taaatagact ggtcgtcatc accgaccacg cacaggttac gatgttcccc
     6301 ggacagcagc aggacaattt catattggac acggttggta tcctgatact catccactaa
     6361 aatgtaacga aacttttgct gatagctgct caagactgca ggctgctcct gcagtagttt
     6421 taccacataa agcagtaagt catcaaaatc taaaacatta tttaagcgta gtcgctcttg
     6481 gtaactcttg tagatacgag ctacagtgcg cgtataataa tcatctgcag tatcttcata
     6541 acgatgctca tcaagcagat cattttttgc ccgactaatg gcagccaaca gaccacgcgg
     6601 cttaaattgc gtactatcga gattaagatc tttcattata ttacgcacca aagtcatttg
     6661 gtccatacta tcataaataa caaaagatga tgtcagacct aacagggcag cattggcgcg
     6721 aatcatacgc acagcagccg catgaaaagt ggaaacccac atccctggga ttgtccccac
     6781 taactgggca acacgtgtac gcatttcttc tgcggcttta ttagtaaagg taagagcgag
     6841 tatttcccat ggtttagcta gctcttgttt aatcagataa gcaatgcggt gggtgatgac
     6901 acgtgtttta ccgctccctg cccctgccaa aaccagcagc ggcccttcta cacactccac
     6961 agcctgacgc tgcatttcat ttaatccatc attgatttgt gtcattacag cctccctgtt
     7021 tcacatatcg ccttcaacta cgcctgcttt tcagtactgt gctcagcagc ttgagataaa
     7081 aagcagcata actgacttgc agaactgatg tagtaggggc gaactgcgtt cgcccgcacc
     7141 ttaattgaag tttagtccta tcttcgcgtt ttttcagtaa tgcgccttgc ttgttcttta
     7201 gggggttacg ccgtaggggc gaactgagtt cgcccgcacc ttaattgaag tttagtccta
     7261 tcttcgcgtt ttttcagtaa tgcgtctttc ctgttcttta gggggttacg tcgtaggggc
     7321 gaactgcgtt cgcccgtacc ttaatttaag tttagttcta tcttcgcgtt ttttcagtga
     7381 tgcgtcttgc ttgttcttta gggggttacg tagaaaaagc agtattaact gcttttcttg
     7441 ttttatgtat tggtttttag ccgctctaac agcagtttgt agccgcccgc accatagttc
     7501 agatatcgtt ttatgcggct aatggtagct gtactggcac ctgtctcctg ctcaatctgc
     7561 tggtaggtat tgccttcctg cagcattttg gctacatgca aacgctgtgc catagcttta
     7621 atttcgttaa tagtacagat gtcttcgaag aactgataac actcttcttc gttttccaac
     7681 agcaaaatcg cttcaaaaag cagatcggta tttttatctt taaattttga actggccaca
     7741 gttacacctc cggaaggtta tcagtttcta tttcgtgatc ccgttaatag tttccttcct
     7801 tactcttaaa ttctttgtaa tcctcttccc aaatattatc tgctttggta aaataacaaa
     7861 aagtgcaact atatgttgtc aaataggggg gctgaaaatg attcgtaagc tgacgatgga
     7921 ggatcatcag caagtagaaa attacttaca ggtgctgcca tcattttcct tgtttctggt
     7981 agcagatatt gaaaagtacg gttatgatag tagcgtcttt acagtctggg gaatatttag
     8041 tcacacaggg gatttactgc atgtttttgg caaatattat cataccctgg ttgtctatag
     8101 taataccgct gcttatcctg cagccgaagt caatgctttc ttaagtgatc aagcgattga
     8161 gtatgacacc atactgggtc gcagtgtttt agtagagccg tttttagcag atctttcctt
     8221 cgctcagatc ttccgtaatt attactgcga attaaacaga gaaaatttta cccctctgat
     8281 taatcaagca ataaacgtaa ctaaagcgga tttatcccag acaaaagcta ttctaaattt
     8341 attagcctcg attaaggaat tccccagggc aagttttcag gaagacgctc tcaacaccta
     8401 tattgcagat gatggagttt atttgattat ggaagatgat gaaattgcct cactggcaat
     8461 gtgttcggct cgcacagcag ggctggctaa tattggtggt gtgtgcacag ctaagccata
     8521 tcgccgcaag gggtatgctt ccacagttgt ctccgctctt tcaaaaaaat atcttgagca
     8581 aggattaatt tgttgtttgg gttacaataa tcccgaggct ggagcaattt atcaacgcct
     8641 cgggtaccaa actgtgggtc atattattat tgccacaaaa taacaagtaa gcttgctgga
     8701 tacagttgct catggcctgc cctcggcact ataacagtag ttctttacta aaaatctggc
     8761 atcaattatc aaacagtaca caaaaaggac cagccggttc tttttgtgtt tatcagaact
     8821 tttcaagaaa aactaaatta tggccatact aaagtcaggc agcaatgtat gccactttga
     8881 ctagaaaaat tcgctaccag gaaaaacaaa ggagatgaaa aaaattaacg accaactaaa
     8941 ggaaatttta tcacacccgg cggatggtgc tgtggctatc gttacacagg gtaaagacgg
     9001 cccccatgtc gttaatactt ggaatagcta catcaagcaa tcggctgatg gcaggtggtt
     9061 ggctcctgcc agtagaatga acaaaacgga agagaatctt gccgctgata accgcgttaa
     9121 actaactgtc agcagtcggg aagttcaagg caagatgtac aaaggaacag gttttttaat
     9181 caccggaacc gcggagtttt taaaagacgg tccagatttc gagacgataa aaggaaagtt
     9241 tgactgggcc cgagctgctt tggccattac tattgagaca gctgaacaaa cactgtaatt
     9301 atgagcgcaa aggcaacaaa gccgccactc tactgcgagt gacggcttta caatttattt
     9361 aatggccttg agacgtgccg gaataaaatc atgccgctgc cgacgttcgc tcatccgctg
     9421 cagttccttt tccatttcca gaaacacagc actccacggc aactttagtt ttttaaccaa
     9481 ggtctcttta accgctaaga taaattgata tttccagaat gtaaaagcag gatcataggt
     9541 taggacaaaa gcacaaaatt taccaatatc ctcttccttg g
//
LOCUS       DASRMS010000207         3543 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_394956, whole genome shotgun sequence.
ACCESSION   DASRMS010000207 DASRMS010000000
VERSION     DASRMS010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_394956"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..287
                     /locus_tag="VFC74_09990"
     CDS             78..287
                     /locus_tag="VFC74_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25695.1"
                     /translation="MTNKTNNQQIKSQFDFILKMLSTKSEHGLALHACGNEQNSYHRG
                     YSDGLNEATELVKVLQTQINILNGE"
     gene            290..1294
                     /locus_tag="VFC74_09995"
     CDS             290..1294
                     /locus_tag="VFC74_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HZK25696.1"
                     /translation="MGYKKKTPANTLQLGTEVLAGASETESLSDRIARYSGGRSRALE
                     MLDYLDELPVIEYQTRYGTRTRNPSPPQVRSGLRNCANYLHFHEYYTIDKIRLASAQF
                     CKQHLICPLCAMRRGTKSLNSYLERFKIIKAAQPHLKLCMLTFTVQNGECLEERFKHL
                     TGTLKKLMQRRNNYNKGVRGAKYSELCKVQGGVYSIETTNIGNGWHPHLHMLCLVDAN
                     VVQHDLRNEWHGLSGDSFMVDVSPIKGDPVDGFMEVFKYAVKFSDLTLEQNWHAAQIL
                     KGKRLLNAFGLFRGVEVPEELTDELLEDLPYWDRFYRFAFGHYQITGERPPQQPPADA
                     "
     gene            1530..1850
                     /locus_tag="VFC74_10000"
     CDS             1530..1850
                     /locus_tag="VFC74_10000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25697.1"
                     /translation="MNELNEYLHYRALAAKHDNAQVFAGLMDSVVESGSAAVPTRQIA
                     TVVAATLVDELDDLLAILKMTKRQFMEMALIDAMQKANEILDGYGVNDFYEDQAALAA
                     GGAE"
     gene            1847..2161
                     /locus_tag="VFC74_10005"
     CDS             1847..2161
                     /locus_tag="VFC74_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25698.1"
                     /translation="MSAPMFTLCGQVMNVYVQPGGTSKKTGEEYDPRDKVQILGSLPM
                     QGGTHKMELITLNTEDARLFEQAINRTVRVAVGFYVTGRTVGYFIPQGSTPKLVTAAA
                     AG"
     gene            2297..2524
                     /locus_tag="VFC74_10010"
     CDS             2297..2524
                     /locus_tag="VFC74_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25699.1"
                     /translation="MSIKDLDLNTNLKLVGLVPLKLTGHCKTYTPTYQEHHFTILEVG
                     DAYQLTGPEHFEQSYMTLKEALQDCYLNWGA"
     gene            2525..3493
                     /locus_tag="VFC74_10015"
     CDS             2525..3493
                     /locus_tag="VFC74_10015"
                     /inference="COORDINATES: protein motif:HMM:NF013603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HZK25700.1"
                     /translation="MHRHKKTPANTLQFDTEALARVSETESLGDRITRYSGGRSRAVE
                     MLDYLDTLEADHGVKKARAGLRTCANYLHFREYYTVDKVRLHSAQFCKQHLICPLCAM
                     RRGAKTLNSYLEKYHLIMAEQPHLKLYMLTFTVQNGECLEERFKHLTGSLRKLMQRRN
                     NYNKGIRGAKYSELCKVQGGVYSIETTNIGNGWHPHLHMICLVDSHVLQHELRNEWNE
                     LTGDSFMVDVSPIKGDHVGGFMEVFKYAVKFSGLSLEQNWHAAKILKARRLLNPFGLF
                     MGVVVPEDLLDEPLEDLPYWDRFYRYAFGHYQITGECPPQQPPANA"
BASE COUNT          988 a          843 c          853 g          859 t
ORIGIN      
        1 cgctcttact tgatactata gacataaagc ggtagcagcc cgccccacgg ggcttacaga
       61 gcagggactt gaagaaaatg actaacaaaa caaacaacca gcagattaaa tcacagtttg
      121 attttatctt gaaaatgctt agcaccaaat cagaacacgg ccttgcattg catgcttgcg
      181 ggaatgaaca gaacagttat cacagaggtt attcagatgg tcttaatgag gccacagaat
      241 tagtcaaagt tctgcaaaca caaatcaata ttttaaatgg ggaataataa tggggtacaa
      301 aaagaaaacc cccgctaaca ctcttcaatt aggcactgaa gtattagcag gggcatctga
      361 gactgaatct ctaagcgata gaatagcacg ctatagtggt ggacggtcaa gagcattaga
      421 aatgcttgat tatctggatg aattaccagt gattgaatac caaacccgct acggaactcg
      481 cacccgcaac ccatcaccac cacaggtacg ctctggcctg cgcaattgtg cgaactattt
      541 acattttcac gaatattaca cgattgataa aatcagactt gcaagcgccc agttctgcaa
      601 acagcactta atttgcccgc tttgtgccat gcgtagaggt acaaaatctt taaacagtta
      661 tttagaacgg ttcaagatca taaaggctgc acagccccac ctaaaactct gcatgctgac
      721 ttttacggtt caaaatggtg aatgcctaga ggaacgcttt aaacacctta caggcacgct
      781 caagaagctc atgcaaaggc gtaacaatta caataaaggc gttcgtggtg ctaaatactc
      841 cgaactctgc aaagtgcagg gcggtgttta ctccatagag acaaccaata taggcaatgg
      901 ctggcatccc catctgcaca tgttgtgctt ggtcgatgca aacgtagtgc agcacgatct
      961 gcgcaacgag tggcatgggt tatcaggcga ttcttttatg gtggacgtta gtccgattaa
     1021 aggcgatccc gtagacggct tcatggaagt ctttaaatac gctgtaaagt tctcagacct
     1081 tacgctagag caaaactggc atgctgctca gatactcaaa ggtaaacgcc tcctgaacgc
     1141 ctttgggctg tttaggggcg tagaagtacc tgaagagctt accgatgaac ttttagaaga
     1201 cctgccctac tgggacaggt tttaccgctt tgcctttggt cattaccaaa ttacgggcga
     1261 acgcccgccg caacaacccc ccgccgatgc ctgatatgtc aagggtcgtg gaataaatag
     1321 gacgcgcttc accgtccgtt ttatgccgcg aaagcccttg tacacacaca aaaccaaaaa
     1381 agcctagccg tagaggggca tggggtgctc ttccccatgc tcctctgccc agtggcgaac
     1441 ggctcttgct tttgcttttg cctttgatta gtatgatttc cagcttatac agaaagaata
     1501 ggcttaaact acttactaga ggctacacaa tgaacgaatt aaacgaatat cttcactacc
     1561 gcgcattagc agcaaagcac gacaatgccc aagtttttgc tggcctgatg gattccgttg
     1621 ttgagtcggg ttctgcggct gttcctaccc gccaaattgc caccgttgtt gctgccacgc
     1681 ttgtcgatga gttggacgac cttttggcta tcctgaagat gaccaaacgc cagtttatgg
     1741 aaatggccct tattgatgcc atgcaaaagg ctaatgagat tcttgatggg tatggtgtta
     1801 acgactttta tgaagaccaa gcagcgttgg ctgcgggcgg tgcagaatga gtgcgccaat
     1861 gtttacgctt tgcggccaag ttatgaacgt ctacgttcaa ccaggtggga caagcaaaaa
     1921 gactggggaa gagtacgacc cacgggacaa ggtgcagata ttgggttctt tgcccatgca
     1981 gggtggcaca cacaaaatgg agctaatcac actcaacacg gaagacgctc gactgtttga
     2041 gcaggccatt aatagaactg tacgtgtggc agtgggtttt tacgttactg gccgcaccgt
     2101 tggttatttt atcccccaag gttcaacccc aaaactggtc actgcggcag cagctggcta
     2161 ggggcgacga ggtgggggga gcgatggcag aaccttggtt gcccgccccc tctccgagcg
     2221 ctcttacttg atactataga catatctcgg tagcagcccg aaccacgggc cttacagagc
     2281 actaaggaat tacaaaatga gcattaaaga cttggatttg aacaccaacc taaagctggt
     2341 tggtcttgtg cctctcaagc tgactggcca ctgcaaaacc tacacaccta cttaccaaga
     2401 gcatcatttc acgattttag aagtcggtga cgcttaccag ctgactggcc cagaacactt
     2461 tgaacagagc tacatgactc ttaaagaagc attgcaagat tgctatttga attggggggc
     2521 atgaatgcat aggcataaaa aaacccctgc taatactctt caattcgaca ctgaagcact
     2581 agcaagggtc tctgagactg aatctctagg cgatagaata acacgctaca gcggcggcag
     2641 gtcaagggct gttgaaatgc tcgactactt ggacacgctg gaagctgacc acggtgttaa
     2701 aaaagctcgg gcaggactgc gcacatgcgc aaattatttg cactttaggg aatattacac
     2761 ggttgataag gtacgtctgc acagtgccca gttctgcaaa cagcatctaa tctgcccact
     2821 ctgtgccatg cgtagaggtg caaaaacact aaatagctat cttgagaagt accacctgat
     2881 aatggccgag caaccccacc tgaaactcta catgctgact tttacggttc aaaacggtga
     2941 atgcctagag gaacgcttca aacacctcac aggctcgctt aggaagctta tgcaaaggcg
     3001 taataattac aacaaaggta ttagaggcgc taaatactcc gagctttgca aggtgcaggg
     3061 cggtgtgtat tcgattgaaa ccacaaacat tggtaatggc tggcatccgc acttgcatat
     3121 gatttgcttg gttgattcac acgtgttgca gcacgagctg cgcaatgagt ggaatgagtt
     3181 aacaggtgat tcttttatgg tggacgtaag tccgattaag ggcgaccacg tgggcggctt
     3241 tatggaggtt ttcaagtacg ctgtgaagtt ctccggcctc tcccttgagc aaaactggca
     3301 tgccgccaag atattaaagg ccagacgtct cttgaacccc tttgggctgt ttatgggcgt
     3361 ggtagtgcct gaagacctgc tggacgagcc tcttgaagac ttgccttatt gggacaggtt
     3421 ttaccgttac gcctttggtc attaccagat aacgggcgaa tgccctccgc aacaaccccc
     3481 cgccaatgcc tgatatgtca agggtcgtgg aataaatagg acgcgcttca ccgtccggtt
     3541 tat
//
LOCUS       DASRMS010000208        27603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_797592, whole genome shotgun sequence.
ACCESSION   DASRMS010000208 DASRMS010000000
VERSION     DASRMS010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 27603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27603
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797592"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..198)
                     /locus_tag="VFC74_10020"
     CDS             complement(<1..198)
                     /locus_tag="VFC74_10020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25701.1"
                     /translation="MTIFYDILWLKCLMMVSGGEALTEPLLRIALRSKEVGAKEVFIP
                     LHLNEYLSPKQYNEFYWPTLKE"
     gene            405..602
                     /locus_tag="VFC74_10025"
     CDS             405..602
                     /locus_tag="VFC74_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019123276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HZK25702.1"
                     /translation="MKGKVKWFNAEKGFGFIEKEEGGDVFVHFSAIQSEGFKTLEEGQ
                     DVEFDVVEGNRGEQAANVVRL"
     gene            791..2185
                     /locus_tag="VFC74_10030"
     CDS             791..2185
                     /locus_tag="VFC74_10030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015360087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HZK25703.1"
                     /translation="MRKSYDVAMIGGGPAAIYAAYELVSKYPDITVAIFEAGNDIYTR
                     NCPLAEKKVNHCINCKSCDVMRGFGGAGAYSDGKYNLTAEFGGWLTDYLPAEDVLALI
                     DYVDQINLAYGAPKDLYGTGNSDLGKRAMAHDLHLLNAKVRHLGTENNLLILKQLYDH
                     LTAHCHLYCNTPVAEILQNEQGFSLQLEGQETVDCKYLIAAPGRAGSEWFAHQCQKLG
                     LSLFNNQVDVGVRVEIPAVVFQHITDDVYEAKLLYRTNQYGDLVRTFCMNPKGYVVAE
                     NTDGIVTVNGHSYRDEKMHSKNTNFALLVSNRFTQPFNEPHQYGKRIASFSNMLGGGV
                     LVQRFGDLVKGRRTNEHRLSQSFTRPTLQATPGDLSLVLPKRHLDNIIEMIYALDKIA
                     PGMANNDTLLYGVEVKFYSSRLQLTEQLETEIPKMFAVGDGAGITRGLSQASASGVHV
                     ARIIGERLQKQQDK"
     gene            2338..3264
                     /locus_tag="VFC74_10035"
     CDS             2338..3264
                     /locus_tag="VFC74_10035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase family protein"
                     /protein_id="HZK25704.1"
                     /translation="MREIQKAALIGLGGIGCAYASRIMDTEPRLLQVVAAAKRAARWQ
                     QGLQINGRHYQFTLTSPHGPAEPLDLILVAVKHYQLPQAILDLQRFVGPETIILSLLN
                     GISSEETLAASFGWEHVLYGLCLSVDAVREGNKIEFSRAGKVYFGESRNEHYSPRVEA
                     VQRFFRRCQIPHEVPLDMRRQQWWKFMINVGLNQLSGVLRAPYGVFQQLPEAAALMQA
                     AMAEVVTLAQALGIDLSSTEIEDFMQQFQTWPAAGKTSLLQDVEAGRKTEVEMFAGEV
                     CRLGRQANVATPINQMLLFLIQTKEKMAQLRC"
     gene            3455..4681
                     /locus_tag="VFC74_10040"
     CDS             3455..4681
                     /locus_tag="VFC74_10040"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004622619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cofactor-independent phosphoglycerate mutase"
                     /protein_id="HZK25705.1"
                     /translation="MKYVVVLGDGMADYPVESLGGRTPLQVAAKPHIDALAAQGYVGM
                     VQTVPAGLAPGSDTANLAVLGYDPRLYYTGRSPFEAASMGVPLADDDLTFRCNFVTLS
                     AAAVYADRVMLDHSADEISSAEAEILLAAVSEQFSTAELAFYPGVSYRHLLVWHGAPT
                     EWQLTPPHDILDRRIGDYLPGGTAAAQVLAMQEASVALLEAHPINIERQKQGLRPANA
                     IWLWGEGTKPQLPAFSEKYGLHGAVISAVDLIKGLGRCAGLDVLEVAGATGNLETNFP
                     GKVQAALAALQSGTDFVYLHLEAPDECGHRQETANKVKSIELLDQQIIQPLCAGLAAA
                     GAPYRLLLLPDHATPLALRTHTADPVPFLIYDSTVQVDQGPTQYDEAAAKDGGQFVGE
                     GYTLMDLFLAPSQGGE"
     gene            4683..6176
                     /gene="thrC"
                     /locus_tag="VFC74_10045"
     CDS             4683..6176
                     /gene="thrC"
                     /locus_tag="VFC74_10045"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005425049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HZK25706.1"
                     /translation="MAEVMYRSTRGESRTVAAAEAIVNGIAADGGLYVPTEIPQLTIP
                     WAELAALSYQDLALQIMRLFLTDYTEAELAAAIEQAYDAKFDTPALAPLATHGSVHFL
                     ELYHGPTLAFKDMALSILPHLLRLAAQKCGLTQEVVILTATSGDTGKAALEGFAEVPG
                     VKIIVFFPEHGVSPVQKRQMVTQAGANTYVIGIEGNFDDAQSGVKAMFTDAQLRQQMQ
                     QAGYVFSSANSINIGRLVPQVVYYYYAYLQLLSQGKVRAEQAINFAVPTGNFGNILAA
                     YYARCLGLPIGKLICASNTNKVLFDFLTTGVYNKDRQFFVTMSPSMDILISSNLERLL
                     YHASGENTAWVKDQMTYLTQQGHYQVPSTVQTAMSSFVGGYATEEATAAAISSVYQQS
                     HYLIDPHTAVAYAVLQQYLADSGDPTPTVVVSTASPFKFAKDVLEAIAGQQPTEDEFT
                     LLSKLAQTAAQPVPAAIRDLPTRPTLHKTVCEKDEMRQVVESILQLS"
     gene            6214..7905
                     /locus_tag="VFC74_10050"
     CDS             6214..7905
                     /locus_tag="VFC74_10050"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006715527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HZK25707.1"
                     /translation="MKKKFADFTYQRPDLTALQQEWHYLTAQFAKATTAAAQEEVVQQ
                     INLHRQEFESLSSLAHIRHTINTTDDYYAAEQDFFDEAEPHYQELITDYYRQLVRSPF
                     KEELALRYGQQLFTLAAMTLKTFSPAVITDLQQENKTVSQYKKLLASADISFAGEKRN
                     LAQLEPFQQAPERATRQAAAEARYSFFSQHCADFDELYAKLVMVRTSIAQKLGFANFI
                     PLAYLRRHRSDYQAEQVAAFRRQIEQHVVPLATQLREKQRQRLGLSELLYYDEGIFHP
                     QGNALPQGTPEELIAQARSMYEQLAPETGVFFAEMTERSLMDLLSRSGKSPGGYCDYL
                     AAFQAPFIFANFNGTAGDVDVLTHEAGHAFQVWLGRQRQIPEYHFATMDAAEIPSMGM
                     EFFAWPYMELFFADQAKQYRYEHLSSAYLFLPYGCLVDHFQHDVYTEPQLKPVQRRRL
                     WRQLEQRYLPHRDYAGQDFLASGGYWFQQGHIFEVPFYYIDYVLAQTCVFQLWQRALT
                     DPQAAWQDYLTFCQAGGSRSFGELLPAANLVSPFVEGVTDKVSQQVQAWLVKNEP"
     gene            7952..9376
                     /locus_tag="VFC74_10055"
     CDS             7952..9376
                     /locus_tag="VFC74_10055"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015044785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HZK25708.1"
                     /translation="MTTFNEFNISEGILKAVSLMGFEEATPIQEKAIPLALAGEDLIG
                     QAHTGTGKTAAFGIPLAERLRVDNPYIQALVVTPTRELAVQVAEELNRIGQFMRIRTL
                     PVYGGQDIGRQIRALKKQPQIIVGTPGRLMDHMRRNTIRLDHIKTVVLDEADEMLNMG
                     FIEDIEMILQQTPSERQTLLFSATMPLAIQRLAQRFMKAPQVIAIRSKEVTVPNTDQY
                     YMVVEEREKLDVLCRLLDTQPPDLAIVFGRTKRRVDELNEALSKRGYSAEGIHGDLTQ
                     ARRDSTMHQFKEGIIDILVATDVAARGLDISGVTHVYNFDVPQDPENYVHRIGRTGRA
                     GKSGMAITFVTPRELNHLHGIEKGTKHKIHRMPIPTMKDAIEGQQRITVDKLVNTLET
                     EDTSYYHAMAVELMTEHDHTELLAAALKLLTKEPDTTPIKLTEEAPHYSRNPRSSNKR
                     FSYPQRRNNKYSSGNRDRNRRPKK"
     gene            9590..11218
                     /locus_tag="VFC74_10060"
     CDS             9590..11218
                     /locus_tag="VFC74_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HZK25709.1"
                     /translation="MKRYIRGLGWTLCLLLVVSVCYFGFRGQAFAEGETPAEGQSLVG
                     LETGESWAKNWDAAGVYGPSEGLQTVSGQTTISAAGVELQNMVINGDLYLQSTIGSGT
                     VMLRNVTVTGELTVLGGGNLTIVNGQVNRLLVNNPGQAVTIAAEGTAAIWSVRVQSEC
                     QLEEKTGEEATGFGHVYSTTAETLSLSGNFPTLTVENVSGAVNFLSGKIGKVYMESQA
                     NDSVLTLGAQAEIEVIDLVSAVRIKGTGTINKAVVLAEGSELDMDAKEYQFAAGKSVL
                     VTGGIVDVEGKHVVTLQDLADVTLAPGKSTSKTVVVEPTDAQVTVTSSNTSAATATLN
                     GSTISLKALAAGKATITVTAVKEGFSTAKETFTVTVSAPALPKVTVQGLSGITLAKGK
                     SATKTVTANPADATLKVTSSNPAVAAASLSGKTITVSAKAAGTTTITVTAAKAGHTSA
                     RATFKVTVPAPQAPPPPAAKVEAVVEKAENSPVIGSTSIRVRLKNTNTPEKYNVTLAG
                     QKMTNRGSYFELAVPANDSLAKKSTAELKALVKF"
     gene            11259..13046
                     /locus_tag="VFC74_10065"
     CDS             11259..13046
                     /locus_tag="VFC74_10065"
                     /inference="COORDINATES: protein motif:HMM:NF013954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HZK25710.1"
                     /translation="MKRWLGILTLLIAIGLCWQGEAAAAINAYVVKDTNKVIYQYTLG
                     DLLTSYNQNSQLWQDFDKRMMQAGVYAVYDDKSLLYVDFVALLNAYNAGMEVTAYTES
                     ATAKEVTMTEIITQVTTAQDGKLVFRETLRSGNIVSAINNASTANEVRQLLVIGADKL
                     ALDLKSFNTLNSYGKNQVSQSVLDSRPAAGYSAADAIKAVFNTAVTSALSKQQAAVQA
                     MNNAGTAAVMQTALNNHAKILELDLTRFAKLTAEQQATLLGIMLNGKPYANAELVTTT
                     YISGLQTVEMTHTITYVDYDHNLSYFVDKQMSQNPQTDLLGGGWRTADRNLVEWFINA
                     ANFVDEMRPAAIIKDGPARMRTGPGTSYDVVCQLEIGSGPYFRINQAQDSAGYTWYRL
                     LTTGSVGWVREDLLTLTEKKGEGIFQFLLLSETTHVDPSEVNAKILKGKGILDGQAEA
                     FISGAETYKINEIFLISLALHESGNGTSTLATGVKYKDKTVYNMYGIGAVDSDPINKG
                     AEYAYNSGWFTPEAAIIGGAKFAGEKYIHNATYQQNTLYKMRWNPDRPGQHQYATDIG
                     WASKQVAGIKKLYDLMSSYVLHFEIPRYQ"
     gene            13278..13796
                     /locus_tag="VFC74_10070"
     CDS             13278..13796
                     /locus_tag="VFC74_10070"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012740050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HZK25711.1"
                     /translation="MKNPEVTIEMEDGGMIKAELALDTAPNTVKNFVSLVQSGFYDGL
                     IFHRVIPGFMIQGGDPQGTGMGGPGYGIKGEFQLNGFKNPLKHARGVLSMARSARPNS
                     AGSQFFIMHADSPHLDGQYAAFGQVTAGIEVVDQIAAAPTGAQDRPQREQRMKKVTVE
                     TFGEEIGEPEKL"
     gene            13926..15086
                     /locus_tag="VFC74_10075"
     CDS             13926..15086
                     /locus_tag="VFC74_10075"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HZK25712.1"
                     /translation="MREVYLDNSATTQVVPEVAAAMQEMLTENFGNPSSLHRRGLLAE
                     RAKKEARRVVAQACDVKEQEILFTSGGTEANNLAIKGVARRWRRRGNHLLTTKIEHPS
                     VLYAYQALEQEGFEVTYLDVDEDGLVTAEAVTAAMRADTLLVSIMHVNNEVGSILPVA
                     EIGPQIKAKNPQTLFHVDAVQSLGKLTLRPHAWQADLLTVSAHKIHGPKGCGALYRKD
                     GLLLSPLLHGGDQEQGVRPGTENMAGIVGFATAVRLILAQQEDNYQQLVKLKQMFSEG
                     LQTRLPEIRQNGPLNGAPHILNLTVPDIKGEVLVHALAEEGIYLSTGSACHAHRAAPS
                     HVLLAMGRSPQQIAASLRLSFSALLTAADVEYALEKITAAVTTLRLMTRRKP"
     gene            15083..16252
                     /gene="thiI"
                     /locus_tag="VFC74_10080"
     CDS             15083..16252
                     /gene="thiI"
                     /locus_tag="VFC74_10080"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014254703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140741 - tRNA-uracil-4 sulfurtransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uracil 4-sulfurtransferase ThiI"
                     /protein_id="HZK25713.1"
                     /translation="MKALYLVRYGEIGLKGKNRRFFENALTANIRRALRGVAECRVQQ
                     RHSRNFVEVLSGDVLAVEARLQEVFGIVSISPVATAPLTLADINVVAQREFSKVAKQG
                     LRFKVNTRRANKQFPLRSPQVSAEVGAHLLESFPALVVDVHEPEAILDVEIREQEAYI
                     YTRRLPGPGGLPVGVAGKGLLLISGGIDSPVAGWMTMKRGVKLEAIHFHSYPFTSERA
                     KEKVIDLCRELARYGNKITLHVAHFTEIQKELRLKTPERLTVTLMRRMMFRIAERLAA
                     QQGALALITGESLGQVASQTMESINVIERVTQIPVFRPLIGMDKQEIMTISRKIETYP
                     ISIRPYEDCCTIFLPDFPATRPRLKDVEAAEASLDLEALIAESLAKTETLEIRAN"
     gene            complement(16314..17504)
                     /locus_tag="VFC74_10085"
     CDS             complement(16314..17504)
                     /locus_tag="VFC74_10085"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011099522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HZK25714.1"
                     /translation="MLSKKAQSITPSPTLVIDAKAKQMVKDGVDVVGFGAGEPDFETP
                     QHIKEAAMQAIRAGFTRYTPVAGALDLREAICEKLHTDNKLQYQPQEIIVSNGAKHSL
                     SNTLAVLLNPGDEVIIPAPFWVSYPEMVKINDGVPVIVPTTAAQHFKLTVTALAQAHT
                     SKTKAVILNSPNNPTGQIYSRAELTELAEFALQHNLYLISDEIYEKLIYGQEEHLSIA
                     ALAPEIKERTIVVNGVSKAYAMTGWRIGYTASTAAIATLMNSVQSHVTSNANAIAQKA
                     TIAALRGPQACVEEMVIAFAQRRDYMVQAVQKLPGLICIEPQGAFYVFIDISHLLGRT
                     YHGQLLGSSVDFAALLLEHAKVALVPGTAFGAPNYLRLSYALSLPQIEKGLARLAQFI
                     AQLV"
     gene            complement(17552..18634)
                     /gene="serC"
                     /locus_tag="VFC74_10090"
     CDS             complement(17552..18634)
                     /gene="serC"
                     /locus_tag="VFC74_10090"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HZK25715.1"
                     /translation="MENRVYNFNPGPATLPLTVLQQAQAELLNYKGTGMSILEMSHRA
                     KEFDEIVLGTEALFKELTGLGDDYRVLFCQGGASQQFAMLPLNFLTQGKSADYLITGS
                     FADKAFKEAKKIGSAQIAATTADDKHNRIPTQEELTLQREAAYVHLTTNNTIYGTEWH
                     YIPQTDAPLVADMSSDILSRPWEYQKFALIYAGAQKNLGPAGVTVVLIRQDMLSDINA
                     ALPTMFNYDTFASKNSMYNTPPTFSIYLLQLVLKWVQAQGGLTVLGQQNKEKAALLYE
                     ALDEDDFYRGHAQVESRSLMNVTFRLPNEELEKAFVAAAAEIGLAGLKGHRSVGGIRA
                     SIYNAMSPQGCETLASFMHDFKKRNR"
     gene            18799..19446
                     /locus_tag="VFC74_10095"
     CDS             18799..19446
                     /locus_tag="VFC74_10095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HZK25716.1"
                     /translation="MQTTIFKGVSILLIRSRETGSLGANCYLVACEQTRLGLLIDPGA
                     DAAQIIEMVQEAGITIKYIVNTHGHVDHVGANAAVREAFAAPIVIHTADSQLYRRPKE
                     SLTLFFGDAELAEADQLVQDGDQLQVGQLAVTVLATPGHTKGSICLLVGDALFSGDTL
                     FAGSIGRTDFPGGSASEIITSIKEKLLVLPPTTKVYPGHGPQTTIGTEKAHNPFL"
     gene            complement(19694..21895)
                     /locus_tag="VFC74_10100"
     CDS             complement(19694..21895)
                     /locus_tag="VFC74_10100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HZK25717.1"
                     /translation="MRRVTRFVLITAVVVFLLSVGAMGFLAATVTGMGEIPDVQTPLT
                     STFYDYEGNVLATRFEQNRFEVPLDKMPKYLPEAFIAIEDHRFYDHFGLDLVGLARAM
                     LRNLRAGRIVEGGSTITQQLAKNLFLTHEQTMTRKLQEMLLTIQLERKFSKDEILEKY
                     LNTIYFGHSAYGVEAAARTYYNKSVQDLTLAEAALMAGITRGPAYYSPYNSEETRAAA
                     FKRQNTILTRMVDEGFIEEQEKKEALAQELVFEGRDQLKADKQFGAYYIDYLINNEIM
                     AKITDYTTDPQIIYRGGLHIYTTLDNQLQQIAEEVFANPGFLPETVKNDQGEDVPLQA
                     ALVALDPADGSIRALVGGRNFQKSQFNRAVNKRSPGSSFKPFTYAAALEQGGYTAATI
                     RESGPVSYKIPGNAEPYQPTEYGERYYGSLRLREAIARSSNIVAVKINEEISPAKTVD
                     LAHRVGITSKLDPVLSLPLGSSEVTPLEMAVGYATFANQGMKVEPRFITKITDTDGRV
                     LYQAGAPQRTAVLEPRIAYLLTEMMTSVMKPGGTASSLGPLVNHPVAAKTGTSQGHRD
                     SYMAGYAPDLVTAVWVGNDDNLSLGWGQTGSVRAGQIWANFMRQALQDTPAKDFERPA
                     GLVEVLICPETGLLHNTRCSLEPIRELFIAGTEPTKKDSWPECQYCPQEPDWNWGDGW
                     WFNRPGRDGDDDDDEPEGQQPPNGENEAEEGNNGNGGGGSFWEELFQELRP"
     gene            22089..22547
                     /locus_tag="VFC74_10105"
     CDS             22089..22547
                     /locus_tag="VFC74_10105"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011876870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HZK25718.1"
                     /translation="MLPTPKKFKIVAAAAEGNHKLTAFDNALLEAGIGNVNLVRISSI
                     LPPSAQQDQDLFLPPGSLVPTAYGAMTCDQPGLKIAAAVGVGFSSDTFGVIMEYSGTC
                     SKEEAEAKIASMLADAFARRGMPLVKTDIRGVEHTVESIGCALAAVTLWY"
     gene            22575..23402
                     /gene="speE"
                     /locus_tag="VFC74_10110"
     CDS             22575..23402
                     /gene="speE"
                     /locus_tag="VFC74_10110"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HZK25719.1"
                     /translation="MELWYTEKQTPHLGITCLLQETLCHFKTPYQDLAVIDTLQFGKM
                     LVLDGMVMTTEKDEFVYHEMLTHIAMQAHPRPQHVLVVGGGDGGVIREVIKYDTVETA
                     TLVEIDEAVVEAAKKYLPTIASGLSDPRVNVLIADGINHIKQAVGQYDVILIDSTEPV
                     GPAVGLFTAEFYQDVYRALKKDGLMVAQTESPFFNRDLIADVNRHLQHVFPLTKLYLA
                     SVPTYPSGLWSFTAASKCYDPLACQEKPVQTKYYNYAVHQGAFALPNFVRELVEQKK"
     gene            23399..24262
                     /gene="speB"
                     /locus_tag="VFC74_10115"
     CDS             23399..24262
                     /gene="speB"
                     /locus_tag="VFC74_10115"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HZK25720.1"
                     /translation="MKSLQAWCEQGGRYLASSDNYQDSRLVLVGAPMDFTVSFRPGSR
                     GGPQAIRAASPGLEEYSLAAGKDLRDASFYDAGDILLPIGNTKRSLQLIEAATAAIFA
                     EQKFPLLMGGEHLISLGALRAAAQHHKNLVVLHLDAHADLRTDYLGEADSHAAVMYHA
                     HQELGLELFQFGIRSATAEELAYANQHTHFYPYNVLEAVRQVLPQLTNCPVYLSLDID
                     VVDPAYAPGTGTPEPGGITAAELLTVVSLLSTLNVVGMDLVEVSPAYDPAGITAMLAA
                     KVIREMILAFA"
     gene            24481..26094
                     /locus_tag="VFC74_10120"
     CDS             24481..26094
                     /locus_tag="VFC74_10120"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011971329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HZK25721.1"
                     /translation="MGTKYIFVTGGVVSSLGKGITAASLGCLLKSRGLKVTIQKFDPY
                     INFDPGTMSPYQHGEVFVTDDGAESDLDLGHYERFIDINLNKSSNVTTGKIYWSVISK
                     ERKGDFLGGTIQVIPHITNEIKARIERAAQHTDVDVVITEIGGTVGDIESLPFLEAIR
                     QWKSDVGRENVMYIHVTLIPYLTKSGELKTKPTQHSVKELRSIGIQPDIIVCRTERPL
                     TQELKDKIALFCDITPGGVIENNDADSIYEVPLLLHKEGMDEIVVKQLGLDDVNCDLS
                     DWKHLVQRIKNLEQRVTIGLVGKYVSLRDAYLSVAEALTHGGYENNCEVEIRWIQAED
                     VEEQGAAALLADVDGIIVPGGFGDRGIEGKIAATSYAREQKIPYLGICLGMHCATIEF
                     ARHVCHWEDAHTTEINPETTHPVIDLLPEQKEIEDLGGTMRLGLYPCKLTAETKAAQA
                     YGEELIYERHRHRYELNNHFRERLAQNGLVMSGISPDDHLVEMIELADHPWFVATQFH
                     PEFKSRPNRAHPLFRDLIKAALQYHVNKK"
     gene            26177..26353
                     /locus_tag="VFC74_10125"
     CDS             26177..26353
                     /locus_tag="VFC74_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HZK25722.1"
                     /translation="MAHVINDECISCGSCEPECPVDAISEGAEKYHIDPELCIDCGAC
                     AEVCPVEAIHPDEE"
     gene            26695..27066
                     /locus_tag="VFC74_10130"
     CDS             26695..27066
                     /locus_tag="VFC74_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HZK25723.1"
                     /translation="MSATIMVVDDRVGIRKLLQEVLQSAGYDVITAASGDEAVVLVQQ
                     NSIDLVLLDMKMSGIDGLDTLTLIKKVAPQIIVLIMTAYEELEVLKEAHRRGAADCIS
                     KPFDIEELKATIARQLRAVSA"
     gene            27152..>27603
                     /locus_tag="VFC74_10135"
     CDS             27152..>27603
                     /locus_tag="VFC74_10135"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013843658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HZK25724.1"
                     /translation="MPFVTLKEVLTAAKLGNYAVGAFNTNNMEIVQAIVEAAEEEKSP
                     VILQASQGALKYAGIRYITAMVQAAVASSTVPIVLHLDHGTSFEQTMLCLKHGFTSVM
                     FDGSKYSLAENIALTRRVAEVAHAMGASIEGELGKIGGTEDDISVDER"
BASE COUNT         7310 a         6130 c         6866 g         7297 t
ORIGIN      
        1 ctcttttaag gtcggccaat aaaactcgtt atattgttta ggcgaaagat attcattcaa
       61 gtgcagcgga ataaagactt cctttgctcc cacctcttta gacctcaagg caattctcaa
      121 cagcggctct gttaaagctt cgccccctga aaccatcatt aggcatttta accatagtat
      181 atcataaaaa attgtcactt tagtatcgcc gtactgtagt gatcaggatt aaataaaatt
      241 attttcacta aaagtttgca tttattattt ttatgtgcta ttcttgtaaa gtattgaaaa
      301 gatgtaacga agcaaacgat tgaattgcct actaggcaaa aagtagtttg acgtcccaac
      361 ctctttttga ttcaaaaaat attacatttt taggaggatt cactttgaaa ggtaaagtaa
      421 aatggtttaa cgcagaaaaa ggttttggat ttattgagaa agaagaaggc ggcgatgtct
      481 ttgttcattt ctctgcaatc caaagcgaag gttttaaaac gctggaagag gggcaggatg
      541 ttgagtttga tgttgtagaa ggcaatcgtg gcgaacaagc ggctaacgtc gtgcgtttat
      601 aaattttaaa accaaaagga catttcctaa acggaaatgt cctttttcta ttgccggata
      661 attattatga gcagcgttaa gcgccaaaac ttttgctgat tttacatatc actggacagt
      721 tggctgtgaa aactgtataa tacatggggt ttgtttcaaa cctagcatct atagtatcgg
      781 agggttttta atgcgcaaat catatgatgt agcaatgatt ggcggcggtc ctgcagcaat
      841 ttatgcagca tatgaactgg tatcaaaata cccagacatc actgtagcta tttttgaagc
      901 aggcaacgat atctataccc gtaactgtcc ccttgccgag aaaaaggtaa atcactgtat
      961 taattgtaag tcttgtgatg tcatgcgtgg ttttggtggt gctggagcct attcagacgg
     1021 taaatataat ctgacagcgg agtttggtgg ttggttaact gattatctac cggcagaaga
     1081 tgtgctggcc ctcattgatt atgtagacca gattaattta gcatacgggg cgccaaagga
     1141 tctgtatggc actggcaata gcgatctggg gaagagagcc atggctcacg acctgcactt
     1201 gcttaatgcc aaggtacgcc atcttggtac agaaaataat ttgctgattc ttaagcaatt
     1261 gtatgatcac ctcactgctc actgccacct ctactgcaat acccccgtgg cagaaattct
     1321 gcaaaacgaa caagggtttt ctctacagct cgagggacag gaaacagttg attgtaagta
     1381 tctgatcgct gccccaggtc gtgcaggttc agaatggttt gcccatcagt gccaaaaatt
     1441 aggtctgtcc ttatttaaca atcaggttga tgttggcgta cgggtagaaa ttcctgcggt
     1501 agtatttcag catattactg atgatgtcta tgaggctaaa ttactatacc gcaccaatca
     1561 gtatggggat ttagtacgta ccttttgtat gaatcccaaa ggctatgtgg tagcagaaaa
     1621 tactgatggt attgtgaccg ttaatggtca tagctatcgg gacgaaaaaa tgcacagtaa
     1681 aaacacaaac tttgccctgc tggtcagcaa tcgttttacc cagcccttta acgagcccca
     1741 tcagtatggt aaacgcattg cttcattttc caatatgctg ggcggtggtg tcctggtaca
     1801 acgctttggt gatttagtta aaggccgcag aaccaatgag catcgtctct cacaaagctt
     1861 tacccgcccc actttacagg caaccccagg tgatttaagt ttagttttgc ccaagcgtca
     1921 tctcgataat attattgaaa tgatttatgc tctcgataag atagcaccag gcatggccaa
     1981 taatgacact ctgctctatg gtgtagaagt caaattctac agctcccgtc tccagctgac
     2041 agagcaacta gagacagaaa tccctaagat gtttgctgtt ggtgatggcg ccgggattac
     2101 acgcggctta tcacaagcca gtgccagcgg cgtccatgta gcacggatta ttggtgaacg
     2161 tctgcaaaaa cagcaagaca agtagctaac taacttggta atgctgacaa gtgctctgta
     2221 ttgcctaatg gtacagagca ctttttgtta acccactccc tgtttacatc cccgctatag
     2281 gcaaacgagc ggggagataa attaagcttc accctagcga gcaaaggcgg tgatttgatg
     2341 cgtgaaatcc aaaaagcggc gttaattggt ttaggtggta ttggttgtgc ttatgccagt
     2401 cggattatgg atactgagcc gcgcttactg caggtggtag cagcagcaaa gcgggctgca
     2461 cgctggcagc aggggctgca aatcaatggc cgccactatc aatttacact tactagtccc
     2521 catggcccag cagagccgct tgatttaatc ctggtggcgg ttaaacacta tcagcttccc
     2581 caggccatcc ttgatctgca gcgctttgtg ggaccagaaa ctatcatctt atccttactg
     2641 aatggtattt ccagtgagga aacgttagca gcaagttttg gttgggagca tgtactttat
     2701 ggcctttgtt taagtgtgga cgcggtgaga gaaggaaata agattgagtt ttctcgtgcc
     2761 ggtaaagtat attttggcga gtcgcgcaat gagcattact cgccacgggt ggaggctgtg
     2821 cagcgctttt ttcgccgctg tcagattcct catgaggtac cgcttgatat gagacgccag
     2881 caatggtgga aattcatgat taacgtgggc cttaatcagc tctcgggtgt cctgcgggca
     2941 ccatatggtg tgtttcagca gctgccggag gccgctgctt taatgcaagc ggcgatggca
     3001 gaagtggtga cactggcaca agcgctgggc attgacttgt ctagcaccga gattgaggat
     3061 ttcatgcagc agtttcaaac ctggccagct gctggtaaaa catcgctgct gcaggatgtg
     3121 gaggcaggtc gcaaaacgga agtggagatg tttgccgggg aggtttgccg ccttggtcgt
     3181 caagctaatg tagccacacc gattaatcaa atgctattgt ttttaattca gaccaaggaa
     3241 aagatggcac aactacgctg ttaaacttaa caccgtttct ttatcggcta gtaaattagc
     3301 tcaagcttgc cttgctttct ttcctcgtcg ctggtgtttt taaggggcaa ggtttgcggc
     3361 gaggcaaagg tggcaatttg gtaggttttt ctagccttta cgtccacttt atgatataag
     3421 tttagcaagg gattttaaag gagtggagtg caggatgaaa tatgtggttg tcttaggcga
     3481 tggtatggca gattatccgg tagaatcttt aggcggcaga acgcctttac aagtcgcagc
     3541 taaacctcac attgatgccc ttgcggcgca aggctatgtg ggcatggtgc agacggtgcc
     3601 ggcggggtta gccccgggca gtgacacggc aaatttagct gtccttggtt atgacccccg
     3661 cctttattat acaggtcgtt caccttttga agctgctagc atgggggtgc cgttagcgga
     3721 tgatgattta acttttcgct gcaattttgt gacactctct gccgcggcag tttatgcaga
     3781 tagagtgatg cttgaccata gtgcagatga aatatcatcg gctgaagcag agattctttt
     3841 agccgcagta agtgaacaat tctctaccgc agagctggct ttctatcccg gggttagtta
     3901 tcgacacctg ctggtctggc atggtgcccc gactgaatgg cagttaacgc ctccacacga
     3961 cattcttgac cgtcgcattg gcgactatct gcctggtggt acagcggcag cccaggtgct
     4021 ggcaatgcag gaagcaagcg tggccctgct ggaggcacac cccattaaca ttgagcgcca
     4081 aaagcaaggc ctacgtccag ctaatgctat ttggctttgg ggtgaaggga caaagccgca
     4141 gctgccggca tttagcgaaa aatatggctt gcatggcgcc gttatttccg cggtagattt
     4201 aattaaaggt ttaggccgct gtgcaggcct ggatgtactg gaagtagcag gtgccacagg
     4261 taacttggag actaactttc caggaaaagt gcaggcagct ttagcagctt tgcaaagcgg
     4321 caccgatttt gtctacctgc acctggaagc accagatgaa tgtggtcacc gtcaggaaac
     4381 ggcaaataaa gtgaagtcca ttgaactgct ggatcagcag atcatacaac cgctatgcgc
     4441 tggactggct gcggcaggag caccatacag actgctgctt ttgccagacc atgcgactcc
     4501 tttagcgctg cgcacccata cagctgaccc cgtacccttt ctaatctatg atagtaccgt
     4561 tcaagttgac caggggccaa cacagtatga tgaagctgcg gctaaggatg gcgggcaatt
     4621 tgtcggggaa ggctatacgc tgatggatct ctttttagcc cccagccaag gaggagagta
     4681 agatggcaga agtaatgtat cgcagtactc gtggtgagag ccgcaccgtc gccgcggctg
     4741 aagctattgt taacggtatt gctgctgatg gcggcttgta tgtgccaaca gagattccac
     4801 agctcactat accatgggcg gagctggccg cactctctta tcaggacttg gccctgcaaa
     4861 ttatgcgcct ttttctcact gattacacag aagccgagtt agcggcggcg attgagcagg
     4921 cctatgatgc caaatttgat accccagcgc tagcaccatt ggcaacacac ggctctgtcc
     4981 actttttaga actttaccac gggccgaccc tggcctttaa agatatggcg ctttccattt
     5041 taccgcatct tttacgcctc gcggcgcaaa aatgtggtct cacgcaggaa gtggtgattc
     5101 tgacagcaac ttctggtgat acgggcaaag ctgctttaga aggttttgct gaagtgccgg
     5161 gtgtgaaaat tattgtcttt ttccccgaac atggggttag tcctgtgcaa aaaaggcaaa
     5221 tggtgaccca agccggggca aatacatatg taattggcat tgaaggaaac tttgatgatg
     5281 cccaaagtgg tgtgaaggca atgtttaccg atgcgcagct gcgtcagcag atgcagcagg
     5341 caggttatgt tttttcttct gctaattcca ttaatattgg ccgcctcgta ccgcaagttg
     5401 tttattacta ctacgcttac ctacagcttt taagccaagg taaagtgcgg gccgagcaag
     5461 caatcaattt tgccgtacct accggcaatt tcggcaacat cctcgccgcc tattatgcac
     5521 gttgtctggg attgcccatt ggcaaactaa tttgtgcttc caataccaat aaggtacttt
     5581 ttgattttct caccactggc gtttataata aagaccggca gtttttcgtt actatgtctc
     5641 cttccatgga tattctcatt tccagtaatc tggagcggtt attataccat gccagcggtg
     5701 aaaataccgc ttgggttaaa gatcagatga cgtacttaac tcaacaaggt cattatcagg
     5761 tgccaagcac cgtgcaaact gccatgtcat cgtttgtggg cggctacgct actgaagagg
     5821 ccacggcagc agcgattagc tctgtttacc agcagtcaca ttatttaatt gatccgcata
     5881 ctgccgtagc ctatgctgtg ctgcagcagt atctggcaga cagcggtgat cctaccccta
     5941 cggtagtagt gtcgacggcc agtcctttca aatttgccaa agatgtactc gaggctatcg
     6001 ctggacagca gcccactgaa gatgaattta ccctgctgag taaattagcc caaacagcag
     6061 cacagcctgt accagcagct attcgtgatt tgccaacaag accaacttta cacaaaacag
     6121 tttgtgagaa agatgaaatg cgccaggtag tagagagtat tctgcagctg tcttaactac
     6181 agctgttgtt ctgcggcggg aaaggagtat tttgtgaaaa aaaaatttgc cgattttacc
     6241 taccaacgac cagatcttac cgcgctgcag caagaatggc attatttaac cgcacagttt
     6301 gctaaagcca ccacagcagc agctcaagag gaagttgtgc agcaaatcaa tctgcaccgc
     6361 caggaatttg agtccttgtc ttcgctggcc catattcgcc ataccattaa tactaccgat
     6421 gactactatg cggcagaaca agattttttt gatgaggcgg aaccacatta tcaggaatta
     6481 attacagact attatcgtca gttggtacgc tctcccttta aagaggaact tgctctgcgt
     6541 tatgggcaac agctttttac tttagcggcc atgactttaa aaacattttc gcctgcggtc
     6601 atcaccgatt tacagcaaga gaataaaaca gttagccaat ataaaaaatt actagcctcg
     6661 gctgacattt cgtttgcggg ggaaaaaaga aatttagctc aactagaacc ttttcaacaa
     6721 gctcccgagc gtgctacacg tcaagccgct gcagaagctc gttatagctt tttttctcag
     6781 cactgtgctg attttgatga actttatgct aaactagtga tggtacgtac ttcgattgca
     6841 caaaagctag gttttgccaa ctttatccct cttgcttatc tgcggcgaca ccgctctgac
     6901 taccaggctg agcaggttgc tgccttccgc cgtcagatag agcagcatgt cgtacccttg
     6961 gccacgcagc tgcgagaaaa acaacgtcag cgcttgggct tgtctgaact gctttattat
     7021 gatgagggta tctttcatcc ccaaggcaac gcactgcccc aaggcacacc agaggaactg
     7081 attgcccagg cccgcagcat gtatgagcaa ttggcaccag aaactggcgt cttctttgca
     7141 gagatgacag aacgcagtct gatggattta ttaagtcgct cgggcaaatc acctggcggc
     7201 tactgcgatt atttagcagc ttttcaggca ccctttattt ttgccaattt taatggtacg
     7261 gccggtgatg tagacgtact cacgcacgaa gccggacacg cttttcaagt ttggctcggc
     7321 cggcagcgac aaataccgga atatcacttt gcgaccatgg atgcggcaga gattccctcc
     7381 atggggatgg agttttttgc ttggccctac atggaactct tttttgccga ccaagctaaa
     7441 cagtatcgct atgaacattt aagcagtgct tatctttttt taccctacgg ttgtttagtg
     7501 gatcattttc aacatgatgt ttatacagaa ccacagctga agcccgtaca gcgtcgtcga
     7561 ctctggcggc agctggagca gcgttatctc ccccacagag attatgccgg acaggacttc
     7621 ttagccagcg gcggctattg gttccagcag ggacatattt ttgaagtgcc cttttactac
     7681 atcgactatg tgctggcaca aacatgtgtg tttcaactct ggcagcgtgc tttaaccgat
     7741 ccccaggctg cttggcagga ttatctgacc ttctgccagg ctggtgggag ccgctctttt
     7801 ggcgagttat tacctgcagc aaatctcgtc tcaccttttg tggagggcgt cacagacaaa
     7861 gtgtcgcagc aggtccaagc ctggttagta aaaaacgaac cctaggtagt aaaaaataag
     7921 tatcctgtag cataagaaag aaggtgtaag tatgacaaca tttaatgaat ttaacattag
     7981 tgagggtatc ctcaaggctg ttagtttgat gggttttgaa gaagcaacac ctattcagga
     8041 aaaagccatt ccgctcgccc tggccggtga agatttaatt ggccaggccc ataccgggac
     8101 aggcaaaact gccgcctttg ggatccccct cgccgaacgc ctgcgagttg ataaccccta
     8161 tattcaggcc ttagtcgtta ctcctacacg tgaactggcc gtgcaggtag cggaagaact
     8221 aaatagaatt ggccagttta tgaggatacg taccttgccg gtgtatggcg gccaagatat
     8281 cggccgacaa attcgcgcgc taaaaaaaca gccgcaaatc attgtgggca ccccaggccg
     8341 tttgatggat catatgcggc gcaacacgat tcggctggat catattaaaa cagtggtctt
     8401 ggatgaagct gatgaaatgc ttaacatggg ttttattgag gatattgaaa tgattttgca
     8461 gcaaacacca tcagaacggc agaccttact tttttccgcc accatgcccc tggctattca
     8521 acgcttggcc caacgtttta tgaaagcacc gcaagtgatc gccattcgtt ccaaggaagt
     8581 tacggtgcct aatacagacc aatactatat ggtcgtcgaa gagagagaaa agttagatgt
     8641 tttatgccgc ctgctcgata cacagccccc ggatttagcc attgtttttg gccgcactaa
     8701 acgccgggtg gatgaactta acgaggcgct cagtaaaagg ggttattcag ctgaaggtat
     8761 ccatggcgat ctcacgcagg cacggcgaga ttccaccatg catcagttta aggaaggaat
     8821 tatcgacatt ttggttgcta cagatgtcgc agcgcgtggt ctagacatca gtggtgtgac
     8881 gcatgtctat aactttgatg ttccacaaga ccctgagaat tatgtgcatc gcatcggccg
     8941 cactggtcga gcgggcaaaa gcggcatggc cattaccttt gtcaccccgc gcgaattaaa
     9001 tcatttacac ggcattgaaa aaggtaccaa acataaaatt caccgcatgc ccattcccac
     9061 catgaaagac gccattgaag gacaacaacg cattacagta gataaactag tgaacacgct
     9121 agaaacggaa gacacttcct attatcatgc gatggcggtt gaattaatga ctgaacatga
     9181 ccataccgaa ttgctggcgg cggccctcaa gctgctgacc aaagagccgg atacaacgcc
     9241 cattaagctg acagaagaag cgccgcatta ttcacgtaac ccgcgctcct ccaataaacg
     9301 cttttcctac cctcaacgac gcaataacaa atatagtagt ggtaatcgcg accgcaacag
     9361 acgacctaaa aaataaaata aaagtaaaaa cctacaaccc ctattgcgga aacagcaata
     9421 ggggtttttc gttgccatcc cttaactagt gctttagtcc caatttttat caatttttaa
     9481 agtggcgcag gaattatgga aactgatgtc gaaatagcca caatcaagct tgctctattt
     9541 atgtctcctg gcaagcttac taaaatctgt gtaaaaaggg gttttaagag tgaagcgata
     9601 cataagaggt ctgggatgga cactgtgtct actcctggtt gtcagcgtgt gttattttgg
     9661 ttttcgggga caagccttcg ctgaagggga aaccccagca gaaggtcaaa gtttagtagg
     9721 tctggagaca ggagaaagct gggctaaaaa ctgggatgcc gctggtgtat atggccccag
     9781 tgaaggactg caaaccgtct caggtcagac gaccatctct gccgccgggg tagagttaca
     9841 gaacatggta atcaacggtg atctgtatct acaaagtacc attggcagcg gtacagttat
     9901 gctgcgtaat gtcactgtca ccggcgaact aacagtttta ggcggcggca atctgaccat
     9961 tgttaacggt caggtaaacc gcctgcttgt caataatcct ggtcaggcgg tcacgatagc
    10021 tgctgaaggc actgcggcca tctggagtgt tcgtgtgcag tccgagtgtc agttagaaga
    10081 aaagactggt gaagaagcaa ccggttttgg tcatgtttat agcactaccg ccgagacctt
    10141 atctctctca ggcaatttcc ctactttaac ggtggagaat gtcagtggtg cggttaattt
    10201 tctttccggt aagattggca aagtgtatat ggaatcgcaa gccaatgatt ctgttttaac
    10261 gctgggggca caagcagaga tcgaggttat tgatcttgtt tctgctgtac gcattaaagg
    10321 aacaggcacg attaacaaag cagttgtttt agctgagggc agtgaattgg acatggatgc
    10381 taaagagtat cagtttgccg caggcaaatc cgttttagtt actggcggga ttgtcgatgt
    10441 tgaaggcaaa catgtggtca cactgcagga tcttgctgat gtgactttgg ctcccggtaa
    10501 atcaaccagt aaaaccgtgg ttgttgagcc gacggatgct caagtaactg ttacttccag
    10561 caataccagt gccgccaccg ctactttaaa cggcagcacg atttccctta aagctctggc
    10621 tgctggtaaa gcaacgatta ccgtaactgc cgtcaaggaa ggtttttcta cggctaaaga
    10681 aacctttacg gtgactgtca gtgctccggc actacctaaa gtaacagtgc aggggcttag
    10741 cggcatcacc ttggccaagg gcaaatcagc tactaaaaca gtaacagcca acccagccga
    10801 tgctacgctt aaagtcacct ccagcaaccc tgctgtcgcc gctgccagcc tttccggtaa
    10861 gaccattact gtcagtgcta aagcagcggg cactacgacg attacggtta cggcagctaa
    10921 agctggccat accagtgcca gagcaacctt taaagttaca gtacctgcac cacaagcacc
    10981 gccgccacca gcagcaaaag tggaggctgt cgtagaaaag gcagaaaata gcccggttat
    11041 cggctctaca agcattcgtg tcaggttgaa aaataccaat acacccgaga aatacaatgt
    11101 tactttagct gggcaaaaaa tgactaaccg aggcagctac tttgaattag ccgtacctgc
    11161 caatgatagt ttggcgaaaa aatctaccgc agaattaaaa gcgctagtga aattttaaag
    11221 ttaccagtaa ctgattaccg gaagaaaggg gggagctcat gaaacgttgg ctggggatat
    11281 tgacattact aattgccata ggcttatgtt ggcagggaga agctgccgcg gcaattaacg
    11341 catatgttgt taaagatacc aacaaagtta tttaccagta cactctaggt gacttattga
    11401 catcctataa tcaaaatagc caactatggc aggactttga taaacggatg atgcaagccg
    11461 gggtttatgc tgtttatgat gataagtccc tgctctacgt agactttgtg gccttgctca
    11521 acgcctataa tgcggggatg gaagtcaccg cttacaccga aagtgccacc gccaaagagg
    11581 tcaccatgac agaaattatt acacaagtca ccactgctca agatgggaaa cttgttttta
    11641 gggaaacttt gcgttctggt aatattgtta gtgccatcaa taatgcgagt actgccaatg
    11701 aggtgcgcca actattagta attggtgcag acaagttggc tctcgactta aagagtttta
    11761 atactcttaa tagttatggt aaaaaccagg taagtcagtc cgtgctagat agccggccag
    11821 cagcaggtta tagtgcagca gatgcgatta aagctgtctt taacactgcg gtcaccagtg
    11881 ccttaagtaa gcagcaggcg gctgtgcagg ccatgaataa tgctggcacc gcagcagtaa
    11941 tgcagactgc tctaaacaat catgctaaaa ttttagaact tgatctcacc cgctttgcaa
    12001 aacttaccgc agagcagcag gctactttgc tagggattat gttaaatggt aagccttatg
    12061 ccaatgctga gctggtgaca acaacctaca tcagtgggct gcaaacagta gaaatgactc
    12121 ataccatcac ctatgtggac tatgatcata atttaagcta ctttgttgat aagcaaatga
    12181 gtcaaaatcc acaaactgat ttattgggtg gtggctggcg tacagcagac cgtaatttag
    12241 tggagtggtt cattaatgcg gctaactttg ttgacgaaat gcggccggcc gcgattatta
    12301 aggatggtcc agctaggatg cgcaccggtc caggcacatc ttatgatgtc gtttgccagc
    12361 tggagatcgg cagtggtccg tatttcagga ttaaccaggc tcaagatagt gccggttata
    12421 cctggtacag attgctgacc acaggcagtg tgggctgggt acgggaagat ctgttaactt
    12481 tgacagagaa aaagggtgaa gggatttttc aattcctgct gctttccgag acaacgcatg
    12541 ttgatcccag tgaagtaaat gccaaaatcc tcaaagggaa aggcattttg gatggtcagg
    12601 cagaagcctt catcagtggt gcagaaacat ataagattaa tgagatcttc ttgatttctc
    12661 tggccctgca cgaaagtggt aatgggacaa gtactttagc cactggtgtc aagtataaag
    12721 ataagaccgt gtataatatg tatggtattg gtgccgttga tagtgatccg attaataaag
    12781 gggcagagta cgcctacaat agtggttggt ttacacctga agcagccatc attggcggtg
    12841 caaaatttgc tggtgaaaaa tatatccaca acgctaccta tcagcagaac acactctata
    12901 aaatgcgttg gaatccggat agacccggac agcaccaata tgccacagat atcgggtggg
    12961 caagcaagca agtggccggg ataaaaaaac tctacgatct catgagctct tatgtgctgc
    13021 atttcgagat cccccgttat cagtagtaaa aaagatcgta aacagggagc aaatgagctc
    13081 cctgttttgc attgccaaat aatagaggca gcggtcttga ctgtgctctt tttgtggtat
    13141 actattactg ttgcgttttg ctgcggccta gtgaatctat gcccgcgcgc tgttaaagga
    13201 tgtttgcctg attttagaaa gcgtctgcag tacttgatgt tacatagttc atttttccta
    13261 agaaaggatg gtcactaatg aaaaacccgg aagtaacaat tgaaatggaa gatggcggta
    13321 tgattaaagc agaacttgct ttggatactg ccccgaatac agttaagaat tttgtctctc
    13381 tagtgcaaag tggtttttat gatggcttaa tctttcatcg tgtcatccct ggttttatga
    13441 ttcaaggcgg tgacccacaa ggcacaggca tgggtggtcc cggctatggg atcaaaggcg
    13501 aattccaact aaatggtttt aagaaccctt taaaacatgc acgcggtgtg ctttctatgg
    13561 cgcgttccgc gcgtcctaat tctgccggtt cccaattctt tatcatgcat gcagattcac
    13621 ctcatttaga tggccaatat gctgcctttg gccaggtaac tgcagggata gaagtggttg
    13681 atcaaattgc tgctgcgccc accggcgccc aagatcggcc gcaaagggaa cagcgcatga
    13741 aaaaagttac agtggagact tttggcgaag agattggtga gcccgagaaa ttgtaaacct
    13801 gttaacggga ggggcgcaca ccgtagcgcc cctcctgctt ggttagggtg cagataaaaa
    13861 taggcaatag ttttgagcaa cttatggtaa aatacaaagg aagtttttgt aggcggggga
    13921 ttagtgtgcg tgaagtatat ctagataata gtgctacgac gcaagtggta ccagaagtgg
    13981 cagcggccat gcaggagatg cttaccgaga atttcggtaa tccctcttct ctgcaccgtc
    14041 gcggcttact ggctgagcga gccaaaaagg aagctcggcg ggttgtcgcc caggcgtgcg
    14101 atgttaagga gcaggaaatc ctttttacct ctggcgggac agaagctaat aatttagcga
    14161 ttaaaggtgt ggcccggcgc tggcggcggc gcggcaacca cttgctcacc accaaaatag
    14221 aacatccttc cgtcttgtat gcctaccagg ctttagagca ggaaggattt gaggttacat
    14281 atctggatgt ggatgaggat ggtttagtca cggccgaggc tgtgactgcc gcgatgcgag
    14341 cggatacact gctagtcagc atcatgcatg tgaataatga agtaggctct atcctgccgg
    14401 tggcggagat tggtccgcag attaaagcca aaaatcccca gactctcttt catgttgatg
    14461 cggtgcagtc gttaggcaaa ttgactttgc ggccgcatgc ctggcaggcg gatttactga
    14521 ctgtttcggc gcacaagatt catggcccca aaggctgtgg tgccctgtac cgcaaggacg
    14581 gcctgctcct atcaccgctg ctgcatggcg gcgaccaaga gcagggggtg cggccgggta
    14641 cggaaaatat ggcaggcatc gtcggttttg ccacggcagt gcgcttgatt ttagcccagc
    14701 aagaggataa ttatcagcag ctggttaagc taaaacaaat gtttagcgag ggtttgcaga
    14761 cacggctgcc cgagatcaga caaaacggcc cccttaacgg tgcaccacac attcttaatc
    14821 ttacagtgcc tgacattaaa ggcgaagtac ttgtacatgc tttagccgaa gaaggtatct
    14881 atctttccac aggttctgcc tgtcatgcac accgtgccgc gcccagccat gttttattag
    14941 caatggggcg ttccccacag caaattgccg cctcgctgcg cttaagtttt tctgctttgc
    15001 tgaccgcagc agatgtggaa tatgccctgg agaaaattac tgcagcggtg acaacactac
    15061 gcctgatgac acggaggaaa ccatgaaggc tttatattta gttcgttatg gtgaaattgg
    15121 cttaaaagga aaaaatcgca gattttttga aaatgcgctg acggctaata ttcgccgcgc
    15181 actgcgtggc gtagcagaat gccgtgtaca gcagcggcat agccgtaatt ttgtcgaggt
    15241 gctcagtggt gatgtcctgg cagtagaagc caggctgcag gaggtctttg gcattgtttc
    15301 catcagccca gtggccaccg caccacttac tttagcagac atcaatgtag ttgcccaacg
    15361 tgagtttagc aaggtggcca agcaaggttt gcgttttaaa gtaaataccc gtcgcgctaa
    15421 taaacaattt cccctgcgtt ctccccaggt tagtgccgaa gtaggcgcac atttactgga
    15481 gagttttccc gctttggttg tggatgtcca tgaacctgaa gctatccttg atgtagaaat
    15541 tcgcgaacag gaagcatata tttatacgcg ccgtctgcct ggtcccgggg ggctgccggt
    15601 aggtgtggcg ggcaaaggat tgctgctgat ctccggcggc attgacagcc cggtagccgg
    15661 ctggatgacc atgaaacgcg gcgttaagct ggaggccatt catttccata gttacccctt
    15721 caccagcgaa agagcgaaag aaaaggtcat tgatttatgc cgtgagctgg cacgttacgg
    15781 caataaaata actttgcatg ttgctcattt cacggaaata caaaaagagc tgcgcttaaa
    15841 gaccccagag cgcctcacag tgactttaat gcggcgcatg atgtttcgta ttgccgaacg
    15901 gttagcagcg cagcaagggg ccctggcgtt aattactggc gaaagcttgg gccaggttgc
    15961 cagccaaacg atggagagta ttaatgtgat tgagcgagtt acgcagatac ctgtttttcg
    16021 gccgctgatt ggcatggata agcaggagat tatgacgatc tcacgaaaaa tagaaactta
    16081 ccccatttcc attcggccct acgaagattg ttgtacaatc tttctgcctg attttccggc
    16141 aacgcgaccg cgtttaaaag atgtggaagc agctgaagcc agccttgact tggaggccct
    16201 cattgcggaa agcctggcaa aaacggaaac attggagatt agagccaatt aaacagtaag
    16261 ttatgagcat taaaaaactg cctgtcgctt ggacaggcag ttttagtttt agtttatacc
    16321 agctgagcaa taaattgtgc cagacgagcc aggccttttt cgatttgcgg caggctgagg
    16381 gcgtaggaga ggcgcagata gttaggtgcc ccaaaggccg tgccgggtac caaagctact
    16441 ttagcatgtt ccagcaggag agcggcaaaa tcaacactgg agccaaggag ctgaccgtgg
    16501 taagtgcggc cgagcaagtg actgatatcg ataaagacat agaaagcacc ttgtggttct
    16561 atacagatga gtccaggcag cttttgcacc gcctgcacca tataatcacg gcgctgggca
    16621 aaagcgatga ccatttcttc cacgcatgct tgcggcccac gtaaagcggc gatggttgcc
    16681 ttttgggcaa tggcattggc gttactggta acatggcttt gcacgctatt catgagggtg
    16741 gcaatggctg ctgtggaggc agtataacca atccgccagc cagtcatggc ataggctttg
    16801 gaaacaccat tgaccacaat agtacgctct ttaatttccg gtgctaaagc ggcaatgctg
    16861 agatgctcct cttgtccgta gatcagtttt tcatagattt catctgaaat cagataaaga
    16921 ttgtgctgca gtgcaaattc agcaagctct gttaattccg cgcggctata aatttgaccg
    16981 gtcggattgt tgggactgtt gagaatgaca gctttagtct tgctggtgtg tgcttgcgca
    17041 agggcagtca cagtaagttt aaagtgttga gctgctgttg ttggtacaat gaccggcaca
    17101 ccatcattaa ttttaaccat ttccggatag ctgacccaaa aaggggcagg aataatcacc
    17161 tcatcacctg ggttcaagag gacggccaaa gtattagaga gtgaatgctt ggcaccgtta
    17221 ctgacgatga tctcctgtgg ttggtattgc agcttgttat cagtatgtag tttttcacag
    17281 atagcctcac gcaaatccag ggcgccggct actggggtgt agcgggtaaa acccgctcgt
    17341 atggcttgca tggccgcttc tttaatatgc tgtggggttt caaagtcagg ctcccctgcc
    17401 ccaaacccga ccacatctac accatccttg accatttgtt ttgctttggc atcgatgact
    17461 aaggtgggtg aaggggtgat ggattgtgct ttttttgata acatgcgccg gatcactctc
    17521 cttttgtgta ctcttatcct atgctaaact gctagcgatt gcgcttttta aagtcatgca
    17581 tgaaacttgc caaggtttca cagccttgcg ggctcatggc attataaatg gaggcacgga
    17641 tgccgccgac tgagcggtga ccctttaagc cagccaggcc aatttccgcc gcggcagcga
    17701 caaaagcttt ttctagctct tcattaggta ggcggaaagt aacattcatc aaactgcggc
    17761 tttccacctg cgcatggcca cgataaaaat catcttcatc gagagcttca taaagcaagg
    17821 ccgctttttc tttgttttgc tgacctaaaa cggtaagtcc accttgtgcc tgtacccact
    17881 tcagtactag ctgcagcagg tagatgctaa aagttggtgg tgtattatac atggagtttt
    17941 tgctggcaaa ggtatcatag ttaaacatgg tgggtaaagc tgcgttgatg tctgataaca
    18001 tatcttgcct gattagcact acagttacac cagctggtcc taaattcttt tgtgctccgg
    18061 catagatcag ggcaaatttt tgatactccc acggacggct taaaatatca ctggacatgt
    18121 cggcgaccaa gggggcatct gtttggggaa tatagtgcca ttctgtaccg taaatagtat
    18181 tgttggtggt aagatgtaca taagcagcct cgcgctgcaa ggttagttcc tcttgtgtgg
    18241 gaatgcggtt atgcttatca tcagctgtgg tagcagcaat ctgggcagag ccgatttttt
    18301 ttgcttcttt aaatgcttta tcggcaaaac tacctgtgat cagataatca gcacttttac
    18361 cttgcgttaa aaagttcagc gggagcatag cgaactgctg actggcgccg ccttgacaaa
    18421 ataaaacacg atagtcatcg ccgaggccag ttaattcttt aaagagagcc tctgtgccga
    18481 gcacaatttc gtcgaattct ttggcacggt gggacatttc taagattgac ataccggtac
    18541 ctttgtaatt cagtagttcg gcttgcgctt gttgtaacac tgttaggggt aaagtagctg
    18601 gacctgggtt gaaattataa acgcgatttt ccattgagtt gcctcccaca taggaatttc
    18661 ctttaattat aagctaagag cagcagaaag ctcaagataa ttcggctgaa aaagtaaaaa
    18721 ttgtgaaaaa aagtgcaaga ggtctgtcaa aagaggtaaa aagcagaaaa ggatttcccg
    18781 tgggcaggtg gaaatactat gcagactact atcttcaagg gggtatctat tttgctcatt
    18841 cgtagtagag aaaccggctc actaggtgcg aactgttatc ttgtggcctg tgaacaaacg
    18901 cgcttaggcc tgctgattga tcccggtgcc gatgccgcgc aaatcattga gatggtgcaa
    18961 gaggcaggga taacgataaa atatattgtc aatacgcatg gacatgtgga tcatgtgggt
    19021 gccaatgcag cagtacggga agcctttgcg gcgccgattg tgattcatac cgccgacagc
    19081 cagttgtacc gcaggcctaa agaaagcctg actttgtttt ttggtgatgc tgagcttgcc
    19141 gaggcggatc agttagtcca agatggcgat caactgcaag ttggtcaatt agctgtgacg
    19201 gtgctcgcta cgccaggtca tactaaaggc tctatttgcc tgttggtagg agatgcgctt
    19261 tttagtggtg atacactttt tgctggttcc atcgggcgca cggatttccc cggcggttca
    19321 gcaagcgaga tcattacctc gattaaagag aaattactgg tgctgccgcc gacaactaaa
    19381 gtttatccgg gacatggacc gcaaaccaca attggcacag aaaaagcgca taatccattt
    19441 ttgtagggcg attgccaaac actcaggatg tgggggcgaa ctgagatgac ccgcatattt
    19501 ttatttttta tgcaccgatg taaggtttgc ataaatagct ttaataggaa cgggcgcaca
    19561 cagtgacgcc cctacgcggg aatgtgtcgg ttgtcgggtg attattgcca aacacctagg
    19621 ttttcggggc gaactgagtt tgcccgcaca gatgaaaaaa atagctggcc gcaggccagc
    19681 tattttaagt tttttagggc cgcagttcct ggaacagctc ttcccagaag ctgccgcctc
    19741 cgccattacc attgttgcct tcttcagcct cgttttctcc atttggcggc tgctggcctt
    19801 ctggttcgtc atcatcatca tcaccatcac gtcccggacg gttaaaccac cagccatcac
    19861 cccaattcca gtctggttcc tggggacagt attgacattc gggccaactg tcttttttcg
    19921 tcggttcagt gccggcaata aagagctccc gtattggttc cagtgagcag cgggtgttgt
    19981 gcaagagacc tgtttctggg cagatgagca cttcgactaa gcctgcaggg cgctcgaaat
    20041 ctttagccgg ggtatcctgc aaggcttgcc gcataaaatt ggcccaaatt tgaccggcac
    20101 ggactgagcc ggtttgtccc cagcccaggg agaggttatc gtcattgcct acccaaacag
    20161 ctgtgactaa atctggcgca tagcccgcca tataggagtc acggtgtccc tgtgaagtac
    20221 cggttttcgc ggctaccgga tgatttacca gcggaccaag tgaggaagca gtgccgccag
    20281 gtttcatgac acttgtcatc atctcagtca gcagataagc aatacgcggt tcaagaaccg
    20341 ctgtgcgttg cggcgcccct gcttgataga ggacgcgacc atcggtatcg gtaattttag
    20401 taatgaagcg tggttctact ttcatccctt ggttagcaaa agtggcgtag ccaacggcca
    20461 tctctaatgg tgtcacttct gaggagccca ggggcagtga aagcacggga tccagtttgc
    20521 tggtaatgcc cacacggtgg gctaaatcca ctgtcttagc gggactgatt tcctcattga
    20581 ttttgacagc gacaatatta ctggagcggg caatagcttc gcgcagccgt aaactgccat
    20641 aataacgttc gccgtattct gtaggttgat aaggttcagc attgccgggg attttgtagc
    20701 ttaccggtcc actttcacgg attgtggctg ccgtataacc gccttgctct aaggcggcag
    20761 cgtaggtaaa aggtttaaag gaagagccag gtgaacgctt gttgacagcg cggttaaatt
    20821 gacttttttg gaagttgcgc ccgccgacga gggcccggat ggaaccatct gcggggtcta
    20881 gggccaccaa agctgcctgc agcggtacat cttcaccttg gtcattcttg actgtctccg
    20941 gcaaaaagcc tggattcgca aagacctctt ctgcgatttg ctgcagctga ttatccaagg
    21001 tggtataaat atgcagaccg ccgcgataaa tgatttgcgg gtcagtggta tagtctgtaa
    21061 tcttggccat aatctcatta tttatgaggt agtctatata ataggcacca aattgtttat
    21121 cggctttgag ctggtcacgg ccttcaaaga ctaattcttg tgctaaagcc tctttctttt
    21181 cctgctcttc aataaaacct tcatctacca tgcgggtgag gatggtattc tgtcgtttaa
    21241 acgccgctgc ccgcgtctct tcactattat agggtgagta ataagcaggt ccgcgggtaa
    21301 tgccggccat caaagctgct tccgccagcg ttaaatcctg gaccgattta ttataatagg
    21361 tacgtgctgc tgcttctacc ccataggcac tatgaccgaa gtaaattgta tttaaatact
    21421 tttccaagat ttcatctttg gaaaattttc gctccaactg aattgttaac aacatttcct
    21481 gtagtttacg cgtcattgtt tgttcatgcg tgaggaataa atttttagcc aattgctggg
    21541 taatggtgct gccgccttcc acaatgcgtc cggcacgtaa attacgcagc atggcgcgcg
    21601 ccagaccgac aagatccaga ccaaagtgat cataaaaacg atgatcttca atggcaatga
    21661 aggcttcagg tagatatttc ggcattttat ccagcggtac ttcgaagcgg ttctgctcaa
    21721 aacgtgttgc caggacatta ccttcataat cgtagaaggt gctggttagt ggtgtttgta
    21781 catcagggat ttcgcccatg ccggtaactg tggccgccaa aaaacccatg gcccccacgc
    21841 taagtaagaa cacaactact gctgtgatta gcacaaaacg ggtaacacgg cgcataccgt
    21901 cacctacttt gtcgaaagat ttttaaactt aaataattat atcacgaatc gctggcaagt
    21961 gctgagtaat tatttgcatc cccggtaaga tgtggtataa tgctttcggt atgcctaaaa
    22021 aagcacattg accggctcaa ttgggcaaca ctagaagcgt atacctttga taaaaggagg
    22081 ctatttatat gttacccaca ccaaagaagt ttaaaatcgt ggctgctgct gctgagggca
    22141 accataaact gacagcattt gataatgcct tactagaagc tggtattggc aacgttaacc
    22201 tggtacgaat ttctagtatt ttaccaccgt ccgcccaaca agatcaggat ttatttttac
    22261 ctcctggttc gctcgtaccg actgcttatg gtgcgatgac ctgtgatcaa ccaggcctta
    22321 aaattgctgc ggcagtgggc gttggctttt cctcagatac ttttggcgtc atcatggagt
    22381 attccggcac gtgcagcaag gaagaagcag aagctaaaat tgcctcgatg ctggcagatg
    22441 cttttgcccg tcgcgggatg cccttggtaa aaacagatat ccgtggtgtg gagcatactg
    22501 tggagtccat tgggtgtgct ttagctgcag ttacactttg gtattaaaac ttaggacagg
    22561 aagggaattg tgccatggaa ttatggtata ccgaaaaaca aaccccccat ttaggtatta
    22621 cttgtctgct tcaggaaaca ctttgtcatt ttaaaacacc atatcaagat ctggcggtta
    22681 tcgacacact gcagtttggc aaaatgcttg tgctggacgg catggtgatg accacagaaa
    22741 aggatgagtt tgtttaccat gaaatgttga ctcacatcgc gatgcaggct catccgcggc
    22801 cacagcatgt tttagtcgtt ggtggtggag atggcggcgt gattcgtgaa gttatcaagt
    22861 atgatacggt agaaacagct actttagtgg aaattgatga agctgtggtg gaagctgcga
    22921 aaaagtatct gccgaccatt gcctccggac tcagtgaccc cagggtaaat gtcttaattg
    22981 ctgatggtat caatcatatc aagcaggcag tggggcaata tgatgttatt ttgatcgatt
    23041 ctacagaacc ggttggtcca gcagtggggt tatttactgc ggaattttac caagatgtct
    23101 atcgtgcttt gaagaaggac ggactgatgg tcgcccaaac agaatcacct ttctttaacc
    23161 gtgacttaat cgccgatgtt aatcggcatc tgcagcatgt cttccctctt accaagctct
    23221 atttagccag cgtgcccaca tatcccagcg gcctgtggag ttttacagca gcttctaaat
    23281 gttatgaccc gcttgcttgc caagagaagc ctgtgcaaac aaaatattat aattatgctg
    23341 tgcatcaagg tgctttcgct ttgccaaatt ttgtgcgtga actggtggag caaaagaaat
    23401 gaagtccctg caggcatggt gtgagcaagg cggccgctat ttagcctcca gtgataatta
    23461 tcaggacagc cgcctggtat tagtaggtgc acccatggat ttcacggtat cttttcgccc
    23521 cggttcgcgc ggcggcccgc aggctatccg ggctgcttca ccaggactgg aggaatacag
    23581 cctggctgcc ggcaaagatt tacgtgacgc tagtttctat gatgccggtg acattttact
    23641 gccgatcggt aatacaaaac gctccctgca gttaattgaa gcggccacag ctgctatttt
    23701 tgccgaacaa aaatttccgc tgctcatggg cggcgagcat ttaatcagtt taggcgccct
    23761 gcgagcggca gcccagcacc acaaaaatct ggtcgtgctg catctagatg cacatgcgga
    23821 tttgcggaca gattatttag gtgaagctga ctcccatgct gcggtgatgt accacgccca
    23881 ccaagaattg gggctggagc tctttcaatt tggcatacgt tctgccacgg cagaggagct
    23941 tgcctatgcc aaccagcata cccactttta cccatataat gtgttggaag cagtgcgcca
    24001 ggtgctgccg cagctgacaa attgtcctgt ctatctttct ttagatatag atgtagttga
    24061 tccagcttat gccccaggga caggtactcc agagccaggc gggatcacag cagcagaatt
    24121 attaaccgtg gtcagcctgc tctcaacctt aaacgtggta ggcatggatt tagtggaagt
    24181 ctcacctgcc tatgatccgg caggaattac cgcgatgttg gcggctaaag taattcgtga
    24241 gatgatcctg gcctttgcct gagcctacca acaaggctag cttgtatgta gtactcataa
    24301 ataatttatg gataataaca caacttaaaa agaggatttt gctgcaaaaa acaggagttc
    24361 ctataataaa gtagtattta ataatatgtt tgaaaaaaac acaacactac gtccgttgca
    24421 aacgccaagg cgcgacgatc acgcgacgat cggatgggga aagggtgaac agataaaatc
    24481 atgggcacaa agtatatctt tgttaccggt ggcgtggtgt cttctttggg gaagggcatt
    24541 acggctgcct cattggggtg tttgctgaaa agcagaggcc tcaaggtaac aatccagaaa
    24601 tttgatccgt atattaactt cgatccgggc accatgagtc cttaccagca tggcgaagta
    24661 tttgtcacag atgacggggc cgaatctgat ttggaccttg gtcactacga acgcttcatc
    24721 gacataaact taaacaaaag ctctaacgtc accacaggaa agatttactg gtctgtaatc
    24781 agtaaagaaa gaaaaggtga ctttttaggc ggtactattc aggtaattcc acacatcacc
    24841 aatgaaatta aagcccgtat tgaacgtgct gcacaacata ctgatgttga tgtggtgatt
    24901 actgaaattg gcggtacggt tggtgatata gaaagcctac cctttttaga agcaatccgg
    24961 cagtggaaaa gtgatgtcgg ccgtgaaaat gtgatgtata ttcacgtcac cttaattcct
    25021 tacttaacta aatcgggtga attaaaaact aaaccaacgc agcattctgt taaggaattg
    25081 cgttcgattg gtattcaacc agacattatt gtttgtcgca cagaaagacc tttgacccaa
    25141 gagctgaaag ataaaattgc cctcttttgc gatattactc ccggtggcgt gattgaaaac
    25201 aatgatgctg attcaattta tgaagtgccg ctgctgctgc ataaagaagg tatggacgaa
    25261 atagttgtaa agcagctggg tttagatgat gtaaactgtg atttaagcga ctggaagcat
    25321 ttagtgcagc ggattaaaaa tctagagcag cgagtgacaa ttggtttagt cggcaaatat
    25381 gtttccctcc gtgacgccta cttgagtgta gcagaagctc tgactcatgg cggttatgaa
    25441 aacaactgtg aggtagagat tcgctggatc caggcagaag atgtggagga acagggagcg
    25501 gcagctttgt tagctgatgt agatgggatt atcgtacccg gtggttttgg cgatcgtggg
    25561 attgaaggca aaattgcggc taccagctat gcgcgtgagc agaaaatacc ctatttgggg
    25621 atctgtttag gcatgcattg tgccaccatt gagtttgctc gccatgtctg ccactgggag
    25681 gatgcgcata ctaccgaaat taacccagag acaacacatc ctgtcattga tttgctaccc
    25741 gagcaaaagg agattgaaga tctaggcggt acgatgcgtt taggacttta tccctgtaag
    25801 cttacagcgg agaccaaggc cgcacaagcc tatggcgaag aactcattta tgaacgtcac
    25861 cgccaccgtt atgaacttaa taatcatttt cgcgaacgcc tggcacaaaa tggcttggtg
    25921 atgagtggaa tttctcctga tgatcattta gtggaaatga ttgagctagc cgatcatcct
    25981 tggtttgtcg ccacccaatt tcatcccgaa tttaaatcca gacctaatcg tgcccatccg
    26041 ctatttcgcg atttaatcaa ggctgcctta cagtatcatg tgaacaaaaa ataaaataat
    26101 gtcaaataca gttgtaatat tagtggttgt gtggtaaaaa gattagtgta taactagtat
    26161 gaaaggaagt gtccagatgg ctcatgtaat taatgatgaa tgtatttctt gcggttcttg
    26221 cgaacctgaa tgtcctgttg atgcaatttc tgaaggtgct gaaaaatacc atattgatcc
    26281 agaactatgt attgattgtg gtgcctgtgc agaagtttgt ccagtggaag ctattcatcc
    26341 ggacgaggaa tagtcatttg cattttagtg tgaacgaaaa gcgtgctgta ggtcgacaag
    26401 cacgcttttt tgcttttttg accttcgtct cttttgcata atgctgatct aaagaggaaa
    26461 aagtagcact gagtcataag catacgcaaa aggaggtcta gctatgttct tccgcatccg
    26521 taaatcacgt acgcgtcgca tgttagattg gttctcctct gtttttgccg gtgtgcgtcg
    26581 ttggttctca aagtagagta tttgccgttt ttacaaaaga ttgtcagcaa gcaggattct
    26641 acttccctag agagaatata tccataagat ggataagaat gagaggtaga aagtgtgtct
    26701 gctacaatca tggtcgttga tgatcgagtt ggtattcgaa aattattaca ggaagtttta
    26761 cagagtgccg gttatgatgt cattacggca gctagtgggg atgaagctgt tgttttagta
    26821 cagcagaact cgattgattt agtcctactc gacatgaaaa tgtcgggcat tgatggtttg
    26881 gataccttaa ctctcattaa aaaagttgca ccacaaatta tcgtactgat catgacagct
    26941 tatgaggaat tagaagtgtt gaaagaagcg caccgccgcg gggctgctga ttgtatttcc
    27001 aagccatttg atattgaaga acttaaagct accatagcta gacagctacg tgcggtaagc
    27061 gcctaaatct aaataaaaat tcaactgtta tctggctgtt aatactgctc agcagcatta
    27121 aacagcctct ttaaggagga agcataacaa aatgccattt gtcaccctga aagaagtact
    27181 cacagcagca aaactaggta attatgccgt cggtgccttt aatactaata acatggaaat
    27241 agtccaggcg attgtggaag ctgccgaaga agaaaaatca ccagtaattt tacaagctag
    27301 tcaaggtgcc cttaaatatg cgggcattcg ctacatcaca gccatggtgc aggcggcagt
    27361 tgcttccagt actgtgccca ttgtcttaca tctggatcac ggtaccagct tcgaacagac
    27421 catgctctgt cttaaacatg gttttacgtc agtgatgttc gacggttcta aatactccct
    27481 agcagaaaat attgccttaa cccgcagagt ggctgaggtg gcacatgcca tgggtgcttc
    27541 gattgagggg gaacttggca aaattggtgg cacggaagac gatatttctg tggatgaacg
    27601 tga
//
LOCUS       DASRMS010000209        16065 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_797946, whole genome shotgun sequence.
ACCESSION   DASRMS010000209 DASRMS010000000
VERSION     DASRMS010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 16065)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16065)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16065
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_797946"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VFC74_10140"
     CDS             complement(<1..870)
                     /locus_tag="VFC74_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25725.1"
                     /translation="MISPEAKKLAEERAARMQAAVELKEPDRVPFTGVGGDVIPAYAG
                     ISAYEYEFDFEKNRQAAIKWFTDFPSDSAPRAGITGIGCRVMPVAFADFPDLADSFGS
                     INGPMNDILKMNFARFPGRELGEESSPQFIGCCNMTPDEYDQLIADPVRFNFEKIVPR
                     IAGNLKDLGSPTALATMARLGMEKARQNVEMAKTIQALKQLGYSFGARGSCLAPLDYI
                     GDQLRDIPNLILDLRRYPDKVKAAAEALTEPLLRIALRSKEVGAKEVFIPLHLNEYLS
                     PKQYNEFYWPTLKE"
     gene            complement(883..1506)
                     /locus_tag="VFC74_10145"
     CDS             complement(883..1506)
                     /locus_tag="VFC74_10145"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25726.1"
                     /translation="MRKELLKSFVDMDEQEAVRIAKELAATENGAAVVLAVCQEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMEFTLPKLQQGDLSNIGTMVLGTVQGDVHNIGK
                     DIYKVMAEAAGIKVIDIGIDVPHEKFVEAIKEHQPDLVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLG"
     gene            complement(1623..2861)
                     /locus_tag="VFC74_10150"
     CDS             complement(1623..2861)
                     /locus_tag="VFC74_10150"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25727.1"
                     /translation="MINPEAKKLAQERDARMKAAIALKEPDWVPFMGLGGDVVAAYAG
                     FTSYEFNFDYEKAHQATIKYLKDFPSDRAMAGMSSLGNLVMTLGFLDFPDLANSFFMV
                     NGPMNDILKVKYCRFPGRELPENSSPQFIGGTYMQPEEYDELIADPVKFSYEKILPRT
                     ITTLQELGSPTALAAMARFGLEIAKQRAAMMRLAQDLAALGYASSSFGAFACAPLDYI
                     GDQLRDIPNLILDLRRYPDKIKAATRSLLETLLSLTLQSKAIGADSAMFPLHLNEYLS
                     PELYKEFYWPTLKEYVLRLREEGIRSQIFFEGRHDAHLDTILELPAGWGSAYFEKTDV
                     RKAKKLLQGHTCIMGGIDVGVVISSTPAKIDEYIKELLGDMMPGGGFILCANVNNLPR
                     ETPVENIRAVYEAVEKYGKY"
     gene            complement(3123..4355)
                     /locus_tag="VFC74_10155"
     CDS             complement(3123..4355)
                     /locus_tag="VFC74_10155"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25728.1"
                     /translation="MVSPEMQKLAAERAARMQAANDLKEPDRVPFRGLGGDVVAAFAG
                     ITAYEYEFDFAKSREAALKWLKEFPSDSPMMGAGGLDLLPMAVAFSDFPDLAGGFMTV
                     NGPMNDILKMKFGRFPGRELDEYSSKQFIGRSNMGPEEYDELIANPQKFSYEKVLPRL
                     SDNFKDMSSPTALATLARAGLERVRQGAEMAKTVQSQMELGFPFGPGGGLSYAPLDYI
                     GDHLRDIPNTVMDLRRYPDKVKAAAESILEVLFKMATANKNAKSITYPLHLNEYLSPK
                     LYQEFYWPTLRSFILRLAEKGIRAGMFCEGWHDPHLETVLDLPAGWGSCSFEKTDVRK
                     AKEVLKGHTCISGGIDVGVIISGTPAIIDEYIKKLLDDMMPGGGYILGPNVGNLPRNT
                     PLENVHAVYEAVEKYGKY"
     gene            complement(4454..5692)
                     /locus_tag="VFC74_10160"
     CDS             complement(4454..5692)
                     /locus_tag="VFC74_10160"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25729.1"
                     /translation="MVSPEMQKLAEQRAARMQAAIELKEPDRVPFGGLGGDVIAAYAG
                     ITAHEYEFDFEKNRAAAIKWLKDFPADSSTGGIGGLDNLAMSVSFVDFPDLANQFFMV
                     NGLMNDALNMKFARFPGRQLDENSSRQFIGGSNMSADEYDELIANPQKFSYEKILPRI
                     ALNLQDLGSPASLAAMARLGMVKAKQAAEMKKTGEAIAALGYPRGGGMTYAFAPLDYI
                     GDHLRDLPNVVLDIRKYPDKVKAATKAILEVLLKFTLQGKAAGAKTASFPLHLNEYLS
                     PKLYREFYWPTLKEYILRVREEGMNSRLFCEGWHDPHLETFLELPAGWGFAAFEKTDV
                     RDAKKVLQGHTCISGGIDVGVIISSTPAKIDEYIKALLGDMMPGGGYILGTNVGNLPR
                     NTPIENIHAVYEAVEKYGKY"
     gene            complement(5704..6327)
                     /locus_tag="VFC74_10165"
     CDS             complement(5704..6327)
                     /locus_tag="VFC74_10165"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25730.1"
                     /translation="MREELLKSFVDMEEVEAVRIAKELVETDGGPVAVLETCKEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMAFTLPKLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGIKVIDIGIDVPHEQFVEAIKEHQPDIVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLG"
     gene            complement(6671..7903)
                     /locus_tag="VFC74_10170"
     CDS             complement(6671..7903)
                     /locus_tag="VFC74_10170"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25731.1"
                     /translation="MVSPEMQKLAQERAARMKAVNDLKEPDRVPFGGLGGDIIAEYAG
                     ISSYEYNFDFDKNRQAIIKWNTDFPADSPQGGVGGLDNLSLSVAFVDFPDLANSIFML
                     NGPMNDVLDMKFARFPGRQLDEDSSQQFIGGKNMEPEEYDALIADPQKFAFETILPRI
                     SGNLKDMSSPTALAAMARLGIVKTQQGFEMMKTGQALAELGFPQGGGFGFAFAPLDYI
                     GDHLRDLPNVVLDIRKYPDKVKAATQSILEVLFKMCMQNVYAETIMFPLHLNEYLSPK
                     LYNEFYWPTLKEFILRLAENGKKSRLFCEGWHDPHLETFLELPVGWGGAMFEKTDVRV
                     AKKVLQGHTVIQGGIDVGVIISGTPAKIDEYIKALLGDMMPGGGYIVGPNVGNLPRNT
                     PMENVAAVYEAVEKYGKY"
     gene            complement(7916..8539)
                     /locus_tag="VFC74_10175"
     CDS             complement(7916..8539)
                     /locus_tag="VFC74_10175"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25732.1"
                     /translation="MREELLKSFVDMDEQEAVRIAKELVETDGGAVAVLETCKEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMAFTLPQLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGINVIDIGIDVPHEKFVEAIKEHQPDIVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLA"
     gene            complement(8680..9918)
                     /locus_tag="VFC74_10180"
     CDS             complement(8680..9918)
                     /locus_tag="VFC74_10180"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25733.1"
                     /translation="MVSPEMQKLAQERAARMQAALDLKEPDRVPFGGLGGDVIAAYAG
                     ISAYEYEFDFDKNRQAAIKWLKDFPADNPTGGIGGLDNLAMSVSFVDFPDLANQFFMV
                     NGPMNDALNMKFARFPGRQLNEYSSRQFIGGSNMSADEYDALIADPVKFSYETILPRI
                     AGNLAELGSPASLAAMARLGIVKAQQAAEMRKTGAALAELGYPQGGGSTFAFAPLDYI
                     GDHLRDLPNVVLDIRKCPDKVKAAAEAILEVLFKFTMQGKAVGAKTAGFPLHLNEYLS
                     PKLYREFYWPTLKDYILRLGEEGMKSRLFCEGWHDPHLDTFLELPAGWGTAAFEKTDV
                     RKAKKMLQGHTCISGGLETGVIISGTPAKIDEFVKRLLGDMMPGGGFILGADVGNLPR
                     NTPIENIQAVYEAVEKYGKY"
     gene            complement(9931..10554)
                     /locus_tag="VFC74_10185"
     CDS             complement(9931..10554)
                     /locus_tag="VFC74_10185"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25734.1"
                     /translation="MRKELLKSFVDMEELEAVRIAKALVETDGGPVAVLETCKEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMAFTLPKLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGIKVIDIGIDVPHEKFVEAIKEHQPDIVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLA"
     gene            complement(10676..11917)
                     /locus_tag="VFC74_10190"
     CDS             complement(10676..11917)
                     /locus_tag="VFC74_10190"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25735.1"
                     /translation="MVSPEMQQLAKERAARMQAALNLKEPDRVPFGGLGGDVIAGYAG
                     ISAYEYEFDLEKNRQAALKWLKDFPADSPTGGLGGLDNLTMSVAFVDFPDLANQFFMV
                     NGPMNDALNMKFARFPGRQLDEYSSRQFIGGSNMSADEYDALIADPVKFSYETILPRI
                     AGNLAELGSPASLAAMARLGIVKAQQAAEMRKTGAALAELGYPQGGGGMTFAFAPLDY
                     IGDHLRDLPNVVLDIRKYPDKVKAATEAILEVLLSFSLQGKAVGATTAMFPLHLNEYL
                     SPKLYREFYWPTLKDYILRLADEGMKSRLFCEGWHDPHLDTFLELPVGWGTAAFEKTD
                     VRVAKKVLHGHTCISGGLETGVIISGTPAKIDEYVKRLLGDMMPGGGFILGADVGNLP
                     RNTPIENVAAVYEAVEKYGKY"
     gene            complement(11930..12553)
                     /locus_tag="VFC74_10195"
     CDS             complement(11930..12553)
                     /locus_tag="VFC74_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25736.1"
                     /translation="MRDQLLKSFVDMEELEAVRIAKELVETDGGPVAVLETCKAAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMTFTLPKLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGIKVIDIGIDVPHEKFVEAIKEHQPDIVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLSKELLG"
     gene            complement(12716..13948)
                     /locus_tag="VFC74_10200"
     CDS             complement(12716..13948)
                     /locus_tag="VFC74_10200"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25737.1"
                     /translation="MTTDPQKLAAERAARYNAAVELKEPDRVPFTGLGGDVVGSYAGI
                     TSYEYCFDFEKSHQATLKYLKDFPSDQPLIPMTSVANTVSSLAFVDFPNVASLVSNID
                     GPTNDVLGVRYGRFPGRELGENSSPQFIGTDYMQPEEYDELIADPVKFCYEKMIPRTT
                     RNLEDLGSPAALATMARLGLEKARQGAAMQKFMRELMELGFPLSGMSMFLAPIDFIGD
                     FLRDIPNLIKDLRRRPDKVKAATEALLEPLLKLVLQGKKTGVEMSFIPLHFNEYLSPK
                     LYQEFYWPTLKQGILSMLEEGMKSRVFFEGRHDPHLETILDLPAGWGIGFFEKTDIRR
                     AKKMLEGHTCIMGGIDTGMVIGSNPAKLDEYIKELLGEMMPGGGFILSPNVSNMPRET
                     PIENIRAVYEAVEKYGKY"
     gene            complement(13960..14583)
                     /locus_tag="VFC74_10205"
     CDS             complement(13960..14583)
                     /locus_tag="VFC74_10205"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25738.1"
                     /translation="MRKELLKSFVDMDEREAVRIAKELVETDGGPVAVLETCKEAMEE
                     VGRLFEAGEYFLSELIAAGDIFNQIMAFTLPKLQQGDVSSIGTMVLGTVQGDVHNIGK
                     DIFKVMAEASGVHVIDIGIDVPHEKFVEAIKEHQPDVVGMCCLITLGVDSMKQTVEVI
                     KEAGLRDGIKIIIGGGRVSEAVMEHVGADAWADDAARGVRLTKELLG"
     gene            complement(14645..15880)
                     /locus_tag="VFC74_10210"
     CDS             complement(14645..15880)
                     /locus_tag="VFC74_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25739.1"
                     /translation="MTTNPQKLAAERAARIKAAYDLQEPDRVPFTGMGGDVVASFAGI
                     TGYQFNFDYELANQAVIKYLQEFSTADQPVGAGSAIVNNVTSLAFLDFPDLAAGLAII
                     DGPINDILGVEIARFPGRELPENSSSQFIGNAYMKPEEYDQLIADPLKFLYETVMTRS
                     TRSLANLGSPASLAAMARYGMERAKKGIASQKFGQELQQLGYPGGARGMFIAPLDFIG
                     DQLRDIPNLIKDLRRYPDKTKAAAKAMLEPLLRLALGAKNAGADLAFIPLHLNEYLSP
                     KLYNEFYWPTLKEGILRMLQEGMKSQVFFEGRHDPHMETILELPAGWGAGYFEKTDVR
                     KAKKLLQGHTCIIGGLDVGMVIASTPAKLDEYIKELLGEMMPGGGFILAPNVGNLPRE
                     TPIENIRAVYEAVEKYGKY"
BASE COUNT         4330 a         4206 c         3097 g         4432 t
ORIGIN      
        1 ctcttttaag gtcggccaat aaaactcgtt atattgttta ggcgaaagat attcattcaa
       61 gtgcagcgga ataaagactt cctttgctcc cacctcttta gacctcaagg caattctcaa
      121 cagcggctct gttaaagctt ccgcagcagc tttaacctta tccggatacc ggcgcaaatc
      181 tagaatcaag ttgggaatat ctcgcagctg gtcaccaata taatccagag gtgctaaaca
      241 tgaacctctg gcaccaaatg aataacctaa ttgcttgaga gcctgaatgg tttttgccat
      301 ttccacgttc tgtctggctt tttccattcc taaacgagcc atggttgcca gggctgttgg
      361 cgatcctagg tcctttaaat tgccggcaat gcgcggtact attttttcaa aattgaacct
      421 caccggatcg gcaattaact gatcatactc atcaggtgtc atattgcagc agccgataaa
      481 ttgcgggctg ctctcttcac ccaattccct gcccggaaaa cgagcgaaat tcatcttgag
      541 gatatcattc attggtccgt taattgaacc gaaactgtca gctaaatcgg gaaaatccgc
      601 aaaggctacc ggcataactc ggcagcctat cccagtaata cctgcccttg gtgcactatc
      661 actgggaaag tctgtgaacc actttattgc tgcctgacgg tttttttcga aatcaaactc
      721 atattcatag gcggaaatcc cggcgtaggc agggatcaca tctccgccta cgcctgtaaa
      781 cggtacacga tctggctcct ttagctcaac tgccgcttgc attctagcgg cacgttcttc
      841 tgctaatttt ttggcttctg gactaatcat tttgttcacc ccttatccca gtaattcttt
      901 acttaaacga acaccacgcg cagcatcatc agcccaggca tcagctccta catgctccat
      961 cacagcttca ctcacgcggc cgccgccgat gataatctta ataccatcac gcaggccggc
     1021 ctctttaatc acttcgactg tctgcttcat ggaatccaca cctaaagtaa ttaagcagca
     1081 catgcctact aagtctggct ggtgctcctt aatagcttct acgaacttct catgcggtac
     1141 atcaataccg atatcaatta ccttgatgcc cgccgcttct gccataactt tgtagatgtc
     1201 tttaccaatg ttgtgtacgt ccccctgcac ggtacctaaa accatggtac caatattact
     1261 cagatcacct tgctgcagct tcggcagggt aaattccata atctgattaa aaatgtcgcc
     1321 ggcagcaatt aattcagaca agaaatactc accagcttca aacaacctgc caacctcttc
     1381 catcgcttct tggcaaacgg ccaaaactac tgctgctcca ttctcagtcg ctgccaattc
     1441 cttggcaatt cttactgctt cctgctcatc catgtccaca aaacttttta gtaattcttt
     1501 acgcatatct ttcacccctg ttttcttaat atcatgatca gcattacact gtcatctcaa
     1561 aaagagcaat tatattacat cttctccact ggctaacagc agggcctact atttattaac
     1621 atttagtact taccgtattt ttctacagcc tcatagactg ctctgatgtt ttccacggga
     1681 gtctcgcgag gcaagttgtt aacattggcg cacaggataa agccaccacc cggcatcata
     1741 tcacctaaaa gctccttaat atattcatcg attttagctg gtgtactact aatcaccaca
     1801 ccaacatcaa taccacccat aatacaggta tggccttgca gcaatttctt cgctttacgc
     1861 acatccgtct tttcaaaata agcagagccc caaccagcag gcagttctaa aatagtatcc
     1921 agatgtgcat catgacgacc ttcaaagaat atttgtgatc taataccttc ctcacgtaaa
     1981 cgcaaaacat attcttttaa ggtaggccaa taaaattcct tgtatagttc tggtgagaga
     2041 tattcattca aatgcagggg gaacatggca ctatctgcac caattgcctt gctctgtaaa
     2101 gttaaactta ataaagtttc cagtaaagac ctggtcgccg cttttatttt atctggatat
     2161 ctgcgtaaat ccagaattaa attcgggata tcgcggagtt ggtcacctat atagtcgagg
     2221 ggagcacaag caaaagcccc aaaagaagaa gaggcataac ctaaagcagc taggtcctgt
     2281 gctagtcgca tcatagctgc cctctgttta gctatctcca ggccaaaacg agccatggca
     2341 gccaaggcag taggggaacc caactcctgc agtgtggtaa ttgtacgggg gagtattttt
     2401 tcgtagctga acttaactgg atccgcaatt aattcatcgt actcttctgg ctgcatataa
     2461 gtaccgccaa taaactgcgg gctgctgttt tctggcagct cccggccagg aaaacgacag
     2521 tacttcacct ttaaaatatc attcatcggg ccgttaacca tgaaaaaact atttgccaag
     2581 tcgggaaaat ccagaaaacc cagagtcata accaagttgc ctaaagacga catacctgcc
     2641 atcgcccgat cactgggaaa gtccttgaga tactttatgg tggcctggtg tgctttctca
     2701 taatcaaaat taaactcgta actagtgaaa ccggcgtaag ctgccacgac atcaccgccc
     2761 aaccccataa agggaaccca gtcaggctct tttaatgcaa tagctgcttt cattcttgca
     2821 tcgcgttctt gtgccagttt ttttgcttcc ggattaatca ttttcttcac cccttgacca
     2881 agattcttta tttaaacgac accacgtgct gcttcgtaag cctaacatca gcatcacata
     2941 atctaaacaa ctgcagctta aacgcgttga cggaattttc aaataactct ttctgcacta
     3001 agtgagctgt ttactgtctg tcaatcttca ccagtatact ttatgtctga tcaagctgct
     3061 gtattccttg tataacgtaa tttaactgac aggggagatc tcccctgtca gttaaataaa
     3121 gattaatact taccgtattt ttctacagcc tcatagactg catggacatt ttccagtggt
     3181 gtgttgcggg gtaagttgcc cacgttaggc ccaaggatat aaccgccacc cggcatcatg
     3241 tcatcaagta gctttttaat gtactcatca attattgccg gagtaccact aatgataaca
     3301 ccgacatcaa tgccgccaga aatacaggta tgacccttca gcacttcttt agctttacgt
     3361 acatctgttt tttcaaagga gcaagaaccc caaccggcag gtaaatccag aacagtttcc
     3421 agatgaggat catgccagcc ctcacagaac ataccggccc taatcccctt ctcagccaga
     3481 cgcaggataa acgatctcag ggttggccaa tagaattcct gatacagttt aggagagaga
     3541 tactcattca ggtggagagg gtaagtaatg cttttagcat ttttatttgc tgttgccatc
     3601 ttaaacaaga cttctaagat agattctgcg gccgctttaa ccttatccgg atatctgcgc
     3661 aaatccatta ctgtattggg gatatcacgc aggtggtcac caatataatc tagcggagca
     3721 taagagagac cgccaccggg gccaaaggga aagcctaatt ccatttgtga ttgtactgtt
     3781 tttgccattt ctgcaccctg tctgactctt tccaggcctg cacgagccaa ggtagccaaa
     3841 gcagtaggtg aactcatatc tttaaaatta tcgctgagac gagggagtac tttttcatag
     3901 gaaaattttt gcggattggc tattaactca tcatactctt ccggccccat attacttctg
     3961 ccaataaatt gcttactgct gtattcgtcc agttctctgc ctggaaaacg accaaacttc
     4021 attttaagga tatcattcat aggtccatta actgtcataa agccaccagc taagtcgggg
     4081 aaatctgaaa atgctaccgc cataggtagt aagtctaaac cacctgcacc catcattgga
     4141 ctatcactgg ggaattcttt aagccattta agcgccgctt cacgactttt tgcaaaatca
     4201 aactcatact cataagcggt aattcctgca aaagcggcaa caacatctcc gcctaaacct
     4261 ctaaacggaa cgcgatcagg ctcctttaaa tcattagccg cctgcattct cgctgcacgt
     4321 tctgctgcta atttttgcat ttctggacta accattttat cactcctttt ctaatagtaa
     4381 aacgctgtta tgtcatgcac aagggtacta gttgagaggg aaactatccc tctcaactgt
     4441 tattcactac agcttaatac ttaccgtatt tttctacggc ctcgtagacg gcatggatgt
     4501 tttcgattgg tgtgttgcgc ggcaggttac caacattggt gccaagaata tagccgccgc
     4561 caggcatcat gtcgcccaaa agggccttaa tgtactcatc aattttagca ggagtactgc
     4621 taatgatgac gccgacatca ataccacctg aaatacaggt gtgaccctgc agtacctttt
     4681 tcgcgtcacg tacatctgtt ttttcaaagg ccgcaaatcc ccaaccggca ggcagttcta
     4741 aaaaggtttc cagatgcgga tcgtgccaac cttcgcagaa taaacgcgaa ttcatgccct
     4801 cttcacgtac acgcaagata tattctttta gcgtcggcca atagaattct ctatacagtt
     4861 tgggagagag atactcattc aaatgcagcg ggaaactggc agtttttgca cctgcagctt
     4921 tgccctgcag cgtaaacttg agtaaaacct ctaaaatagc tttagttgct gcttttactt
     4981 tatcggggta tttgcggata tcaagcacca cattgggaag atcacgtaag tggtcaccga
     5041 tataatccag cggtgcaaaa gcataggtca taccgccgcc ccgtggataa cctagtgccg
     5101 ctatagcctc ccctgttttc ttcatttctg cggcctgttt agcttttacc atacctaaac
     5161 gggccatagc tgccaatgaa gcaggtgagc ctagatcctg caaattgaga gcaatacgag
     5221 gcagaatttt ctcgtaactg aacttctgcg gattagcaat taactcatca tactcgtctg
     5281 ccgacatatt actgccacca ataaactgtc ggctgctgtt ttcatccagc tgtctgcccg
     5341 ggaaacgggc aaacttcata ttaagggcat cattcatcag tccgttaacc ataaaaaact
     5401 ggttagctag atccggaaaa tcgacgaacg acactgacat ggctagatta tctaacccgc
     5461 caatgccccc ggtggaacta tcagcgggga agtctttaag ccatttgatg gcagcagcac
     5521 ggtttttttc aaaatcgaac tcatactcat gagcagtaat accggcataa gcggctatca
     5581 catcgccgcc taaccctcca aacggcacac gatcaggctc tttcaattcg atagccgcct
     5641 gcattctggc tgcacgttgt tctgctaatt tttgcatttc gggactaacc attttgtcac
     5701 cccctatcct agtaattctt tacttaagcg aacaccgcga gcagcatcgt ctgcccaggc
     5761 atcagcgccc acatgctcca tcacggcttc gctgacacgg ccgccgccaa taataatttt
     5821 aatgccgtcg cgtaggccag cttctttaat cacttccact gtctgcttca tggaatccac
     5881 acccaaggta attaagcagc acataccgac gatgtccggc tgatgctcct tgatcgcttc
     5941 cacaaactgt tcatgcggta catcaatgcc aatgtcgatt actttgatgc cagaggcttc
     6001 ggccattact ttaaaaatat ccttgccaat gttgtgcacg tcaccttgta ctgtacctaa
     6061 gaccatcgta ccaatactgc tgacgtcgcc ttgctggagc tttggtaggg taaacgccat
     6121 aatttggtta aatatgtcac cggcagcaat taattctgat aagaaatact caccagcttc
     6181 aaatagcctg ccaacctctt ccatggcctc tttgcaggtc tccaaaaccg ctactggacc
     6241 gccatcggtc tcaaccaatt ctttggcaat tctgacagct tcgacctctt ccatgtcgac
     6301 aaaactcttt aatagctctt ctcgcattaa aattccacct ccgtttttta tctgcacccg
     6361 gaaaagagaa tttttccaca gtttcgtaga tagtcctgat attttcacta ggagtttcac
     6421 atgaatatta tcttgggaca tctctacgta actgtatact tttaaatccg acagatcagc
     6481 tatcttcctg gcatggcgca acatggtata aaatgacaga ggacttatcc cctgtcagct
     6541 gctaatttaa tataagatat atttgctata ctattttaca gttaacaggg tgccttgata
     6601 tctaacgcca gctgacagct aacatcttat acaactgaca ggggacttat cccctgtcag
     6661 ttgtacaaaa ttaatactta ccgtattttt ctacagcctc ataaacggca gctacgtttt
     6721 ccattggtgt gttgcgcggc aagttgccta cgttaggacc aacgatataa ccgccacccg
     6781 gcatcatgtc acccaagagg gccttaatgt actcatcaat tttagcggga gtgccactaa
     6841 taatgacgcc gacatcaata ccgccctgaa taacggtatg accttgtagt acctttttcg
     6901 cgacacgaac atctgttttt tcaaacatcg caccgcccca gccaactggt aattctaaga
     6961 aggtctccag atgggggtca tgccagcctt cgcagaataa gcgggatttt ttcccattct
     7021 cagctaaccg caggatgaat tcctttagtg ttggccaata gaattcatta tacagcttcg
     7081 gagagagata ctcattcaag tgcaagggga acatgatagt ctccgcatac acattctgca
     7141 tacacatctt aaataaaact tctaagattg attgagttgc ggcttttact ttatctgggt
     7201 atttacggat atcaagtacg acattaggca gatcacgtaa atggtcaccg atatagtcta
     7261 ggggagcaaa agcaaaacca aagccaccgc cttgtggaaa acctaattcc gccagagctt
     7321 gcccagtttt catcatttca aaaccctgtt gagtttttac tatccctaaa cgagccatgg
     7381 ctgccaaagc agtcggtgaa ctcatatctt ttaagttgcc gctgatgcgg ggcagaatcg
     7441 tctcaaaagc aaacttctgc ggatcagcga ttaaagcatc gtactcttcc ggttccatat
     7501 ttttgccacc gataaactgc tggctgctgt cctcgtcaag ctgcctgccc ggaaaacgag
     7561 cgaacttcat gtccagtaca tcattcatag gaccattaag cataaaaatg ctattggcca
     7621 aatccggaaa atcaacaaag gctaccgaca gtgataggtt atctaaccca ccaacgccgc
     7681 cttggggact atccgcaggg aagtctgtgt tccattttat aatggcttgg cggtttttat
     7741 caaaatcaaa gttgtattca taactggaga tgccggcata ttcagcaata atgtcgccgc
     7801 ccaaacctcc aaacggtacg cgatcaggtt cttttaaatc attaaccgct ttcattcttg
     7861 ctgcacgttc ttgtgctaat ttttgcattt caggactaac cattattttc cctccttacg
     7921 ctagtaattc tttacttaaa cgaacaccac gagcggcatc gtctgcccaa gcatcagcgc
     7981 ccacatgttc catcacagct tcgctgacac ggccaccacc gataataatc ttaatgccat
     8041 cgcgcaggcc tgcttcttta atcacttcta cagtctgttt catggaatcc acaccgaggg
     8101 taatcaagca gcacatgccg acgatgtccg gctgatgctc tttgatagct tccacaaact
     8161 tttcatgcgg tacatcaatg ccgatatcaa ttacattgat gccagaagct tctgccatta
     8221 ctttaaagat gtccttacca atgttgtgca cgtcaccttg tactgtacct aaaaccatcg
     8281 taccaatact gctgacgtcg ccctgctgga gctgcggtag ggtaaacgcc ataatctggt
     8341 taaaaatgtc gccggcagca attagctctg ataagaaata ctcaccagct tcaaatagcc
     8401 tgcctacctc ttccatggct tctttacagg tttccaaaac cgctactgcg ccgccatcgg
     8461 tctcaactag ttctttagca atccttactg cctcttgctc gtccatgtcg acgaaacttt
     8521 tcaacaattc ttctcgcatg cgtgtcacct ccaagttttt ttaatctgaa tagcattaca
     8581 atgttatgtc tcaaagaagg gttatattac acccattgac gctgaataac cggccatgct
     8641 gacagggact tcccctgtca gctgccagtt aaagacaatt taatacttac cgtatttttc
     8701 tacagcctca tagactgctt gaatgttctc aattggtgtg ttgcgcggca agttgccgac
     8761 atcggcgccg agaataaagc cgccgcctgg catcatgtcg cctaagagcc tcttaacaaa
     8821 ctcatcaatt ttagccggcg taccgctaat aatcacgcct gtttccagac cgcctgaaat
     8881 acaggtatga ccctgcagca tttttttcgc tttacgcaca tctgtctttt caaaggcagc
     8941 tgttccccaa ccggcaggta gttctaagaa agtatccaga tgtggatcat gccagccttc
     9001 acagaataac cgggatttca ttccttcttc acctaaacgt aagatgtaat cttttagcgt
     9061 cggccaatag aattctctgt acagttttgg cgagagatac tcattcaaat gcagcgggaa
     9121 accggcagtt ttggcaccta cagctttgcc ctgcatcgtg aatttaaata atacttctaa
     9181 aatggcttca gctgccgctt ttactttgtc ggggcacttg cggatatcaa gcacaacgtt
     9241 aggtagatca cgtaaatggt ccccaatata atctagaggc gcaaaagcga aggttgaacc
     9301 gccaccctgc ggataaccta attccgctaa agccgcacca gtctttctca tttcagcagc
     9361 ctgttgagct ttgactattc ctaaacgggc catggctgct aaagaagcag gagagcctag
     9421 ctccgctaaa ttgccggcga tccgaggtag aattgtctcg tagctgaact ttaccggatc
     9481 ggctattaaa gcgtcatact catctgcgga catattgctg cccccaataa actgtcggct
     9541 gctgtattca ttcagctgcc ggcccggaaa acgagcgaac ttcatgttga gggcatcatt
     9601 catggggccg ttaaccatga agaattgatt agctaagtca ggaaagtcca caaaggatac
     9661 tgacatggca agattgtcta aaccaccaat gccgcctgtg ggattatcag ctgggaaatc
     9721 tttaagccat ttaatagccg cctgacggtt tttatcgaaa tcaaattcat attcataagc
     9781 gctaattccg gcataagcag ctatcacatc gccgcctaac cctccaaacg gtacacgatc
     9841 aggctctttt aaatcaagag ctgcttgcat tctagctgca cgttcttgtg ctaatttttg
     9901 catttcagga ctaaccatta ttttccctcc ttacgctagt aattctttac ttaaacgaac
     9961 accacgagcg gcatcgtctg cccaagcatc agcaccgaca tgttccatca cagcttcgct
    10021 gacacggcca ccaccaataa taatcttaat gccgtcgcgt aggcctgctt ctttaatcac
    10081 ttctacagtc tgtttcatgg aatctacacc cagggtaatt aagcagcaca tgccgacgat
    10141 atccggctga tgctctttga tcgcttccac aaacttttca tgcggtacat ctataccaat
    10201 gtcaattacc ttgatgccag aagcctctgc cattacttta aagatgtcct taccaatgtt
    10261 gtgcacgtca ccttgcactg tacctaagac catcgtacca atactgctga catcgccctg
    10321 ctggagcttg ggtagggtaa acgccataat ctggttaaaa atgtcaccgg cggcaattaa
    10381 ttctgataag aaatactcac cagcttcaaa aagcctgcca acctcttcca ttgcttcttt
    10441 acaggtttcc aaaaccgcta ctggaccgcc atcggtctca accaatgctt tggcaatcct
    10501 tactgcctct agctcttcca tgtccacaaa actctttagt aattctttac gcatactttt
    10561 tcctcccatt tttcgatttg tgttgattta cagcacactt ggataatact ttttacgctg
    10621 acactgacga tgctggcagg ggttaacccc cagccagccg ccagtaaagt actaattaat
    10681 atttaccgta tttttctaca gcttcataga cggcagcgac gttttcaatt ggtgtgttgc
    10741 gtggtaagtt gcccacatcg gcgcctagaa taaagccgcc gcctggcatc atgtcgccta
    10801 agagcctctt cacatactca tcaatcttgg ccggtgtgcc gctaatgatt actcctgttt
    10861 ccagaccgcc cgagatacag gtatgaccat gcagtacttt tttcgcgaca cgtacatctg
    10921 ttttttcaaa ggctgcagtg ccccagccaa cgggtaattc taaaaatgta tctagatgcg
    10981 gatcatgcca gccttcacag aatagccttg atttcatgcc ctcgtcggct aaacgcaaga
    11041 tataatcttt taaggtcggc caatagaatt cccgatacaa ttttggcgag agatactcat
    11101 tcaagtgcaa ggggaacatc gcagtagttg cgccaacagc tttaccttgc agggaaaaac
    11161 tgagcaatac ttctaaaatg gcttcagttg ctgcttttac tttatcgggg tatttacgaa
    11221 tatcaagcac aacgttaggc agatcacgca aatggtcacc aatataatct agtggtgcaa
    11281 aagcaaatgt catgccgcca ccaccctgcg gataacctaa ttccgctaaa gctgcccctg
    11341 tttttctcat ttcagcagcc tgttgagctt tgactattcc taaacgggcc atggctgcta
    11401 aagaagcagg agagcctagc tccgctaaat tgccagcgat ccgaggtagg attgtctcat
    11461 aactgaactt tactggatcg gcgattaacg catcatactc gtctgcggac atattactgc
    11521 cgccgataaa ctgacggctg ctgtattcat ccagttgccg gcccggaaaa cgggcgaact
    11581 tcatgttgag ggcatcattc atcgggccat taaccatgaa aaactgatta gctaaatcgg
    11641 ggaaatctac aaaggcaaca gacatagtca ggttatctag accaccaagg ccccccgtag
    11701 ggctgtcagc tgggaaatct ttaagccatt taagtgccgc ctgacgattc ttctctaaat
    11761 caaattcgta ctcgtaagca ctaatgccgg cataaccagc gatcacatcg ccgcctaaac
    11821 ctccaaaggg tacacgatct ggctctttta aattaagagc tgcctgcatt ctagccgcac
    11881 gttcttttgc taattgttgc atttctggac taaccactaa tatcacctct tatcctaata
    11941 attctttact taaacgaaca ccacgtgcag cgtcatctgc ccaggcatca gcgcccacat
    12001 gctccatcac agcttcgctg acacggccgc cgccgataat aattttaatg ccgtcgcgta
    12061 aaccggcttc tttaatcact tccactgtct gtttcataga atccacaccc aaggtaatta
    12121 agcagcacat cccaacgatg tcgggctgat gctccttgat agcttccacg aacttctcat
    12181 gcggcacatc aattccaata tcaattacct tgatgcctga agcttctgcc attactttaa
    12241 aaatgtcctt gccaatgttg tgtacgtcgc cctgcacggt acctaagacc atggtgccaa
    12301 tactactgac atcgccctgc tgaagcttcg gtagggtaaa cgtcataatc tggttaaaga
    12361 tgtcaccagc agcaattaat tctgataaga aatactcacc agcttcaaat agcctgccaa
    12421 cctcttccat agctgcttta caggtttcca aaacggctac tgggccgcca tcggtctcaa
    12481 ccaattcttt ggcaatcctt actgcttcta gctcctccat gtccacgaaa ctctttaata
    12541 attggtcacg cattacactc tacacctccg ttttttatct agatctgaaa agggagtact
    12601 cccctccgat atctaaaaca gccgtaccgc tttagatccg aaagattgcc ttgcgctgat
    12661 gaagatggtg atcttgccga caggatgtat ccaccctgtc agcaagatac ttactttagt
    12721 acttaccgta cttctccaca gcttcgtaga cagcccggat attctcaatg ggagtttccc
    12781 gtggcatatt gctgacattg ggagaaagaa tgaaacctcc acccggcatc atttcgccca
    12841 aaagttcctt aatgtactca tctagcttgg cagggttact accaataacc atgcccgtat
    12901 caatgccacc cataatgcag gtatgtcctt ctagcatttt tttagctctg cggatatcag
    12961 tcttttcaaa gaagccgata ccccagccgg cgggtaagtc taaaatcgtc tctaggtgcg
    13021 gatcatgacg accctcaaag aaaactcgtg atttcatacc ctcctcaagc atacttaaga
    13081 tcccttgctt gagagtcggc cagtaaaatt cctgataaag ctttggtgat agatactcat
    13141 taaaatgcaa ggggataaag gacatttcta cccctgtttt tttgccctgc aaaacaagtt
    13201 ttaaaagggg ttctaacagt gcttcagttg cagccttaac tttatcagga cgtctacgta
    13261 aatccttaat tagattagga atatcgcgca gaaaatcgcc aataaaatct atgggagcta
    13321 aaaacattga catgcctgaa aggggaaaac ctaattccat cagctccctc ataaactttt
    13381 gcatggctgc tccctgccga gccttctcta aaccgagtct ggccatggta gccaaagctg
    13441 caggtgaccc caaatcttct aaattcctgg tagtacgagg tatcatcttt tcataacaaa
    13501 actttacggg atctgcaatc aattcatcat actcttccgg ctgcatataa tcagtgccga
    13561 taaattgagg actactgttt tcgcctaatt ctctcccggg aaaacgaccg tacctaacac
    13621 ccaggacatc attagttgga ccatctatat tagaaacaag cgaagctaca ttagggaaat
    13681 ctacaaatgc taaggacgaa acagtatttg ctacactagt catgggaatc aacggctggt
    13741 cactaggaaa gtcttttaaa tatttaagcg tggcctggtg agatttttca aagtcaaaac
    13801 aatattcata gctggtaatc cctgcataag agcctactac atcgccgcca agacctgtaa
    13861 aaggtacccg atcaggctct tttaactcaa cagctgcgtt atatcttgcc gcacgttcag
    13921 ctgccagctt ttgtggatct gtcgtcatgt tgtctccccc taccctagta attctttagt
    13981 taaacgaaca ccacgcgcag catcatcagc ccaagcatca gcgcccacat gctccatcac
    14041 agcttcacta acacggccgc cgccgataat aatcttaatg ccgtcgcgca ggccagcttc
    14101 tttaattacc tctactgtct gcttcatgga atccacaccc aaagtaatta aacagcacat
    14161 cccgacgacg tccggttgat gctctttgat cgcttccacg aatttctcat gcggtacatc
    14221 tatgccaatg tcaatgacat ggacgccgga agcttccgcc attactttaa agatgtcctt
    14281 accaatattg tgtacgtcac cttgaacagt acctaagacc atggtgccaa tactactgac
    14341 atcgccttgc tggagcttgg gtagggtaaa cgccataatc tggttaaaaa tgtcaccggc
    14401 agcaattaat tctgataaga aatattcacc ggcttcaaac agtctgccaa cctcttccat
    14461 cgcctctttg caggtctcca aaaccgccac tgggccgcca tcggtctcaa ctagttcttt
    14521 ggcgatcctt accgcttctc gctcgtccat atcgacaaaa ctctttaaca actctttacg
    14581 catttaagaa attcacctcc ggttttttta tctatagcca ataaagggat ttaacccatc
    14641 agttttagta cttaccatat ttctccactg cttcgtagac ggctctaata ttctcaatcg
    14701 gagtctctct tggcagatta ccaacgttag gagcaagaat aaaaccgcca ccaggcatca
    14761 tttcacctaa aagctcttta atatactcat cgagcttggc aggagtacta gcgataacca
    14821 tacccacatc aagaccaccg ataatacagg tatggccttg caacagtttt ttggctttgc
    14881 gtacatcggt cttttcgaag taaccggcac cccagcccgc aggcagctct aaaatagtct
    14941 ccatatgcgg atcgtgacga ccttcaaaga agacttgtga tttcatgcct tcctgcagca
    15001 tccgtaggat gccttcctta agagttggcc aataaaactc attatagagt tttggtgaca
    15061 gatactcatt aagatgcagg ggaataaaag cgaggtctgc acctgcgttt ttagcgccca
    15121 gcgccagtct taaaagcggc tccagcattg ctttcgccgc agctttagtt ttatcaggat
    15181 atcggcgcag atccttaatt aagttaggaa tatcacgcaa ttgatcgccg ataaaatcca
    15241 aaggtgcgat aaacatgcct cttgccccac cagggtagcc caactgttgt aattcttgac
    15301 caaacttctg cgaagcaatt ccttttttgg ccctttccat tccgtaacga gccatggctg
    15361 ccaatgaagc aggagatccc aggttcgcta aacttcttgt agaacgggtc atcactgttt
    15421 catacaaaaa cttgagggga tcggcaatta attgatcgta ctcttccggt ttcatgtacg
    15481 cattgccgat aaattgtgaa ctgctgtttt cgggcaattc tcgtcccggg aagcgggcga
    15541 tctcgacacc cagaatatca ttaatgggcc cgtctatgat cgctaaacct gcagctaaat
    15601 caggaaaatc caagaaagca agcgaggtga cattattaac aatggctgaa cctgcgccca
    15661 ccggctgatc tgcggtagaa aactcttgaa gatatttaat gactgcctga ttagccaatt
    15721 catagtcaaa gttaaactga tagccggtaa tgccggcaaa agaagctacc acatcaccgc
    15781 ccatacctgt aaagggcacc cgatctggtt cttgtaagtc gtaggcggcc ttgatgcgcg
    15841 ccgcacgttc agctgccagc ttttgtggat ttgtcgtcat gttgtctccc cctaccctag
    15901 taattcttta gttaaacgaa caccccgtgc agcatcatca gcccaagcat cagcgcccac
    15961 atgctccatc acagcttcac taacacggcc gccgccgata ataatcttaa tgccgtcgcg
    16021 caggccagct tctttaatta cctctactgt ctgcttcatg gaatc
//
LOCUS       DASRMS010000210         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_533954, whole genome shotgun sequence.
ACCESSION   DASRMS010000210 DASRMS010000000
VERSION     DASRMS010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_533954"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /locus_tag="VFC74_10215"
     CDS             <1..1351
                     /locus_tag="VFC74_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HZK25740.1"
                     /translation="FILFTVGVVLQSQRARTTLLASSGENRAALAQRYSQAGMIFSAD
                     GEKLAYSDTDGHRSYSSDPLLAMAALHVVGDYTHNIAYTVESLWQNELLGEGRSFADQ
                     LKLDLLGQGLQGNDLILTLRSDLMKKAYSLLDGRRGAAVILNYETGAILASVSSPSVH
                     PDKVVAWQDIPDTALFNRAFSGEYVPGSTFKIITSAALLQSPFSTEDTVICQGTTPLM
                     PGGVSETHINAGHGEVSLQAAMTQSCNHYFGFMGLKLGPKRIISAAEQMAFNQKIELD
                     RFHLAKSQCLIGREDDPALTWAAIGQAAGEDKITVSPLHLALISGMIANNGRMVEPYL
                     VESMVYPSAVETNVSKRGSLMAQIDPGLAQELTELLRQVVSSGTGVNAQVPGLEVCGK
                     TGTAEGFNENGELTISSLFTGFSSNSDYPFAVAVVLDDSSAGSSAIAGQLLAAAASN"
     gene            complement(1359..1994)
                     /locus_tag="VFC74_10220"
     CDS             complement(1359..1994)
                     /locus_tag="VFC74_10220"
                     /inference="COORDINATES: protein motif:HMM:NF025644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHHW family protein"
                     /protein_id="HZK25741.1"
                     /translation="MDNKNNNSIKKKPMRTPQLRVASSKNESVKEADQSNAYVNPWIS
                     SDSSVSEVKTTGQEHHEKPRRRPIRKIYKNRTTHLNSIIIYATFMVALVAVGIMTLLI
                     PDKDISTRENRALAQFPKISSESILSGKISSDLDSWYSDQFPGRDGLVTIWKKIDGAL
                     SLQFTDDDITIVEGSDDLGQGALDPDAPEDQSDPLYTSPFFGGGEGENPNP"
     gene            complement(2004..>2357)
                     /locus_tag="VFC74_10225"
     CDS             complement(2004..>2357)
                     /locus_tag="VFC74_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25742.1"
                     /translation="YTWMTFILGWVFFKMESMAGIRILLQRMFQPRSDFVSNRGIVLL
                     QNHLVFLVAAFVFAMPLLPWLRSKKTVQRAMQNLKTMEPLYGISSSVFYMSILFFCTM
                     SLVASGFNPFLYFRF"
BASE COUNT          624 a          500 c          579 g          654 t
ORIGIN      
        1 gtttattctg tttacggtag gagtcgtttt gcaaagccag cgtgcgcgaa caactttatt
       61 ggcatcctca ggagaaaaca gagcggctct ggctcagcgc tattcacagg cgggcatgat
      121 ttttagcgca gatggcgaaa aactggctta ctctgataca gacggacaca ggagctatag
      181 cagcgaccca ttgctggcta tggctgcgct acatgtggtc ggggattata cacataacat
      241 agcttatacg gtcgagtcct tatggcagaa tgaacttttg ggtgagggga gatccttcgc
      301 agatcagctt aagttggatt tgctgggcca gggtctgcag ggcaacgatc tgatactaac
      361 tttgcgtagc gacctgatga aaaaagctta ttcgttgctg gatggcagac ggggtgctgc
      421 cgtaatatta aactatgaga ccggagcaat tttagcctcg gtttcttcgc cctcagtgca
      481 ccctgataaa gttgtagcct ggcaggatat tccagacacg gctcttttta atcgtgcttt
      541 cagtggcgag tatgtacctg gatctacttt taaaataatt acatcagcag ctttactgca
      601 aagtcctttt tctacagaag acacagtcat ttgccagggt actacgccgc ttatgcccgg
      661 aggcgtgagc gaaactcata ttaatgcggg tcacggtgaa gtcagcctac aagctgccat
      721 gacgcagtca tgtaatcatt attttggatt tatgggtttg aaattgggac ccaaaagaat
      781 aatcagtgca gctgaacaaa tggcatttaa ccaaaagatt gaattggatc gctttcatct
      841 ggcgaaaagt caatgtttaa ttgggagaga agatgacccg gctctgacct gggctgctat
      901 tggccaggcc gcaggtgaag ataaaatcac agtcagcccc ctgcatctcg ccttgatatc
      961 aggaatgata gctaataacg gacgtatggt agaaccttac ctggtagagt ctatggttta
     1021 cccttcagct gtcgaaacca acgtcagcaa gcgtgggagt ttgatggcac agattgatcc
     1081 gggcttggct caggaactca cagaattgct gcgccaggta gtaagcagtg gtacaggtgt
     1141 taacgcgcag gtaccggggc ttgaagtatg tgggaaaacg ggtacagctg aaggatttaa
     1201 tgaaaatgga gagttaacaa tttcaagtct tttcaccggt tttagcagta acagcgacta
     1261 tcctttcgca gttgcagtgg ttctggatga ttcatcagcc ggatcatccg ctatagcagg
     1321 tcaacttctg gctgctgccg ctagtaattg aagttggatc agggattggg attttcccct
     1381 tctcctccac cgaaaaaagg actggtgtaa agtggatcgc tctgatcctc cggagcgtcc
     1441 ggatccagag ctccctgacc cagatcatcg ctgccttcaa caatggttat atcgtcatca
     1501 gtaaattgca ggctgagcgc cccatcaatt tttttccata tcgtaaccaa accgtcacgg
     1561 ccggggaact ggtcggaata ccaactgtca agatcgctgg agatttttcc ggataatatt
     1621 gattcgctgc ttatctttgg gaattgagcc aaagctctgt tttcacgtgt ggagatatct
     1681 ttatcaggaa tcagcaaggt cattattcca actgcaacca gggcaaccat aaaagttgca
     1741 taaataatga tgctgttcag atgtgttgtt ctgtttttgt atatctttct gattggtcgt
     1801 ctgcggggtt tttcgtggtg ttcttgtccg gtggttttga cctcagaaac cgaagaatca
     1861 gaactgatcc agggattaac ataagcatta ctttggtcag cttctttaac gctttcattt
     1921 ttgctggagg caactcgcag ttgtggagtt ctcattggtt ttttcttgat cgagttatta
     1981 tttttgttat ccatgtaata ctcctaaaac ctgaagtaga gaaaggggtt aaagcctgat
     2041 gctaccagtg acatggtgca gaaaaaaagt atgctcatgt aaaagacaga actgctgatt
     2101 ccgtacaatg gttccatggt tttcaagttt tgcattgcgc gctgaactgt ttttttgctt
     2161 cgcagccagg gcaaaagggg catagcaaac acaaaagcag caactaaaaa caccaggtgg
     2221 ttctgcagca gcactatgcc gcgattgcta acaaagtcgc tgcgaggttg aaacatcctt
     2281 tgcagtaaaa ttcttatacc tgccatagac tccattttaa agaaaaccca gcctaaaata
     2341 aaagtcatcc aagtgta
//
LOCUS       DASRMS010000211         1032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_397575, whole genome shotgun sequence.
ACCESSION   DASRMS010000211 DASRMS010000000
VERSION     DASRMS010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_397575"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            466..657
                     /locus_tag="VFC74_10230"
     CDS             466..657
                     /locus_tag="VFC74_10230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25743.1"
                     /translation="MKSQITILEIALESNFAIATAYNWAKHPDFPKGVKAAYHQKTTY
                     CRADVKAWLKKRYHREVLA"
     gene            654..965
                     /locus_tag="VFC74_10235"
     CDS             654..965
                     /locus_tag="VFC74_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25744.1"
                     /translation="MNSHESLIKDMDGFGRIAAALSNAIFVTTSSIEQSEAVEGFTRQ
                     CNKMMQAFDNAIDNTDPALHESLAEVAADEHGRVVDMANIIVQGTLDESIQVLMQGDL
                     L"
BASE COUNT          317 a          229 c          250 g          236 t
ORIGIN      
        1 cgggtgaagt tagctgtaga agcccagctg gagatttttc aatactggag gatacggcgg
       61 aaaacgcaaa cgccgcgaaa agattgtttc tagagtgggc taacaatctt cagagagcag
      121 atcagtgaaa catatacgca gagctgcgca tgccgcgtat accgtgggtt gcagcggttt
      181 tatggtttat tgttatctgt agaatatgca gatagaataa gaccagccta ttttggtagg
      241 caaaaaaaag ccccatcata aagacagggc aattttaaca ttcaaattca tggccacggt
      301 aatggccaga catctaaaac cagcctgcaa gcaacgtttt agaagtaaaa agcggttagg
      361 ctttgagcat attgtataca catacacaca gtactgcaaa gccaaaaaca cccccgcctt
      421 taattctaat attttgcttg agctggttaa cataaggctc aagacatgaa atcacaaatc
      481 accatcttag aaattgcgct cgaaagcaat tttgcaattg ctaccgctta caactgggca
      541 aaacacccag acttcccgaa aggcgttaag gccgcatacc accagaaaac cacttattgc
      601 cgcgctgatg ttaaagcatg gttgaagaag cgctaccatc gtgaggtgct ggcatgaaca
      661 gccacgaatc actaattaaa gacatggacg ggttcggtcg cattgctgct gcactgagta
      721 atgctatttt cgttaccacc tcaagcattg agcaatccga agccgtggaa ggttttacac
      781 gccagtgtaa taagatgatg caggcgttcg acaacgcaat tgataacaca gacccagctc
      841 tgcatgagtc actggcagaa gtggcagcag atgagcacgg gcgcgtggtg gatatggcta
      901 acatcatcgt acaaggtacg ctcgatgagt ctattcaggt gctgatgcag ggtgatctgt
      961 tatgagtgcc attacacgcg ggcaagccga agccaagcgc acagaaattg cagatattgg
     1021 ccagcgtatc ag
//
LOCUS       DASRMS010000212         5688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_664248, whole genome shotgun sequence.
ACCESSION   DASRMS010000212 DASRMS010000000
VERSION     DASRMS010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5688
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_664248"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="VFC74_10240"
     CDS             <1..221
                     /locus_tag="VFC74_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25745.1"
                     /translation="SLLELSYKWLLSRKAVDSIICGVSKFSHITQNMEFFKDNAAEAE
                     NTALSASVLAQCDEAWQLLKGKYWNYHY"
     gene            complement(506..1861)
                     /locus_tag="VFC74_10245"
     CDS             complement(506..1861)
                     /locus_tag="VFC74_10245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZK25746.1"
                     /translation="MSVSKPLSNKVINLFSLGLSPFMILMGPVGAYFPYFLTDIVKVG
                     AATVGGILLLTRIADAVSIVLVGGVISKTNLKWGKYCSWIYIGAPLTLVFISLLFTNP
                     TFLSMSAKTIYYCVAYILGSFALNFAWSGASSITPNMAVDPKSRVLLSARRMQFSTVA
                     RLFYSASHMSIILYFGRTNEGAGYFAFIVMAGFLQLFGYLLAASLAKPYDIKKAVPGS
                     TPSLGDMWKQVAGNPPLLVLMLYGVCNGTILSIAGGLNVYYFKYVANNMLLISVFLTA
                     SSIVGMVATVAGQFVAQKIESKRTVMMIGAVISLISYIAAYFFARNTILYMACRWLGA
                     FAGLAGVVNFANYSDVADYAEWKSGKNAKGLVMSISALPMKIASAVAGAITGFGLAAI
                     GYSAAAAATPQLIKGIILLSTLLPAVCAMLGIIILTFYKLDNKTMAKIHEELAARKAT
                     A"
     gene            2549..3703
                     /locus_tag="VFC74_10250"
     CDS             2549..3703
                     /locus_tag="VFC74_10250"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HZK25747.1"
                     /translation="MTQVVIKECSDYQLDKVKQQLNESIMLLGGWKRLVSAGMKVLLK
                     VNLIGPKTPDSAAVTHYEFVRALTQLLQEQGCTVWIGDSSGGAIAGIAPTASSFKVSG
                     LEKVAKDEGAVIKNFDKEGVIEVKTGNAEQMYLAKPMFEADFVINLPKLKTHSAAIYT
                     GAVKNLFGCVPGLKKGEYHRQAPDPQDLGMIIVDIHQSAPVGLHIMDGIMAMQGEGPT
                     AGEVYSAKKILMSTDPLALDTVAAAMLGMAVDDIPVLVSARARKLGESDLQSIEITGD
                     YAEPPALPGFKYPKRFRSSKKRNNSAVIKVIDFLKARPKIDTKRCKQCNMCVESCPVK
                     AIDKESKKINYNTCIECMCCHELCLHKAIDLKNDNWLAALFMRFYRKRAE"
     gene            3767..4423
                     /locus_tag="VFC74_10255"
     CDS             3767..4423
                     /locus_tag="VFC74_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25748.1"
                     /translation="MPKLKSNLLKLVLVLFSFSILASLLPQVILPIASVYGANVAGED
                     IVLFIYYSGFNPFAVSAITAGQLLFSAWYLIRVGRLKEFISWVTSQIPQQSTATRWQK
                     VLIIGLLLVGILGLRSVSSLRTHEQMAPLEYDAKIEIDFADHNNGTIYEFALDDYQVY
                     HFVYSLDTKDPVTLKLTTMNPSGFLYTPGKIDYHVPGCRKTAEGPVHRFHPRTWRLSD
                     "
     gene            4347..4832
                     /locus_tag="VFC74_10260"
     CDS             4347..4832
                     /locus_tag="VFC74_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25749.1"
                     /translation="MYQGAEKLQKGQFTGFILEPGDYQIELDALNQLGELTMYISSTP
                     PTTEEYQFLEIIKALRDGSFTAAHYQEPGYELIYHGELKDVGNQLLYTLNQSGTFLQI
                     SSFVVGTYEELDLRYLEENREFHLLDDKNCTIGYGFDAKAKGELRLSTVGAELYIYAK
                     K"
     gene            complement(4868..5350)
                     /locus_tag="VFC74_10265"
     CDS             complement(4868..5350)
                     /locus_tag="VFC74_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25750.1"
                     /translation="MKLGQLKKITLLLLAVLFFLTGCVDLSNSDPEALGVLGYTDPNF
                     WATQSAVPISITANEGGTASGAGWSNETDEGIYADSAELVAATTLGSSVNVATETTGK
                     NLVLLSESGEVTVPLTNIGALGDNYDALANIETVKAALQADLNAAFPKTNPAALTFTV
                     "
BASE COUNT         1720 a         1218 c         1196 g         1554 t
ORIGIN      
        1 tgagtctgct ggaactttca tacaaatggc tgctttcacg caaggctgtg gacagcatta
       61 tctgcggcgt cagcaagttc agccacatta cgcagaatat ggagtttttc aaagacaatg
      121 ctgcagaagc tgaaaacact gccttatctg caagtgttct ggcgcagtgc gatgaagcat
      181 ggcaactttt gaaaggtaaa tactggaact atcattattg atttccctct cacaggtaac
      241 ccctgataga cggacacggt ataaaatgat gtccgtagta ttatcactac accctctaca
      301 taaaaaaaca gccctatctg ccctcaccca catcaggtga aaaagcaagt agggctgttt
      361 gctatatcta gattcattct agtgctttac ttgatgagaa attgctcatc ttggaaatta
      421 cagagcttct tgcggagaat ttccaagtaa gcgtaatgtg caagtctgtc cgaaagttca
      481 tatttagcgt attgtaaagt tcaggctacg ctgttgcttt tctagcagcc agttcctcgt
      541 ggattttagc catggtttta ttgtcgagct tataaaaggt caagattatg atgccaagca
      601 ttgcacaaac tgccggcagc aatgttgata acaaaatgat gccttttatg agttgagggg
      661 tggcagctgc tgcagcactg tagccaatcg cggctaaacc aaaaccggta atggctcctg
      721 caacggctga ggcaattttc attggtagag cgctgatgga cataactaaa cctttagcat
      781 ttttacctga cttccattct gcgtagtcgg ccacatcaga gtaattggca aagttaacaa
      841 caccagccaa acctgcaaaa gctcccagcc agcggcaggc catatataat atggtgttac
      901 gagcaaaaaa gtacgccgct atatagctga tcaaactaat gacagcacca atcatcatca
      961 ccgtacgctt gctttctatt ttttgagcta caaattgccc agccaccgtt gctaccatcc
     1021 ccacgatgga ggaagcggtc agaaagacag aaataagcag catattattt gctacatatt
     1081 taaaatagta gacatttaac ccaccggcta ttgagaggat cgtcccattg cagacaccat
     1141 aaagcatcaa aacaagcaaa ggcggattac cggccacttg tttccacata tcacctagag
     1201 aaggtgtgct gccaggaaca gcttttttaa tatcatatgg ttttgccaaa cttgcagcga
     1261 gtagataacc aaatagctgc agaaagccag ccataactat aaaagcaaaa taaccggcac
     1321 cctcgtttgt gcgcccaaaa taaaggatga tcgacatatg gctggcgcta tagaatagcc
     1381 tggccacggt agaaaattgc atccgtcttg cagaaagcaa aaccctcgat tttgggtcta
     1441 cggccatatt gggtgtgatg ctggaggcac cactccaagc aaaattcaag gcaaaagaac
     1501 ccaaaatgta tgcgacacag taatagatgg tttttgccga catactcaga aacgtaggat
     1561 tagtaaaaag cagcgagata aataccagcg ttaaaggcgc gccgatataa atccaggagc
     1621 aatatttacc ccattttaag ttcgtttttg aaatcacacc accgactaag acaatcgaaa
     1681 cagcatcggc aatacgtgtt agtagtaata tccccccaac agttgccgca cctactttaa
     1741 caatatctgt gagaaagtag ggaaagtatg cccccaccgg acccatgaga atcataaacg
     1801 gtgaaagccc aagactaaac aagttaatga ccttattact taacggttta ctcactgaca
     1861 ttacaactgc ctcctttttc ctcgttatct ttacccaaac ctgttgatct gccagcctgt
     1921 acctcaatca ctgttcagac tcaatctgct ttcttaggtg aaatattaga atcatactgg
     1981 tacttttaag tgggcaagga cagcgaggat aattgttggc aagagtagtt gattaattgc
     2041 accagctgtt gaagtattct ggctactagc attatatact tgggaatctg atttagaagt
     2101 aaagtctgga tattaattcc acattcctta cggcacggta acacaggctc cgggttcatt
     2161 ttacatattt ttcggcgttg ttttttgtcc tataaagctg tagtttgcga agtgttgagc
     2221 agcacctaat tatgtaaaat tacggcaaga tgtattccaa tcggctttaa cagtcaaaaa
     2281 cgattgtttt acagcggaac aggttatagc caattcctgc ttaattttga atcattatat
     2341 ttagttaaga tttagactgg caggagtcaa gtctctcaac aataaaaacg cactcagcct
     2401 agtacccgga gatccttccg ccataggtta ggcaaagaca gcaaaagaaa tcatagcaga
     2461 taaaagatgg cataaacttg ccgatatcaa ccagcaagtg ctaaaatatc ggtatgaaga
     2521 attgtaaaac taaacaggtg gtgacagcat gacgcaagtt gtgattaaag agtgcagcga
     2581 ttatcagcta gacaaggtaa agcaacaact taacgaaagt atcatgctct taggcggctg
     2641 gaagcggctt gtctcagcgg gcatgaaagt tttactgaaa gtaaatttga ttggacccaa
     2701 aactcccgat tctgctgctg taactcatta tgaatttgtt cgtgcgctaa cacagcttct
     2761 gcaagagcag ggatgtacag tctggatagg tgatagttca ggcggcgcca ttgcaggaat
     2821 agcgcctaca gccagtagct ttaaagtatc tggactagag aaagtagcaa aagatgaagg
     2881 cgcagtgatt aaaaactttg acaaagaggg tgtcatcgaa gtaaagacag gaaacgcaga
     2941 gcagatgtat ctagctaagc cgatgtttga agcagacttt gtcatcaatt tgccaaaact
     3001 taaaacgcac tccgcagcta tttacaccgg ggcggtgaaa aacctctttg gttgcgtccc
     3061 cggtttaaaa aagggcgaat accatcgtca agctcctgat ccgcaagatt tgggtatgat
     3121 catagtagat attcatcagt cagcgccagt cggactacat atcatggatg gaattatggc
     3181 aatgcaaggg gaaggtccaa ccgcgggaga agtttactct gcaaagaaaa tcctcatgag
     3241 cacagatccc ttagcccttg atacagttgc agcggcaatg ctcggcatgg cagttgatga
     3301 tattcccgtt ttggtcagcg ccagagcacg aaaacttggt gaatccgatt tgcagtcaat
     3361 tgaaatcaca ggtgattatg ccgaacctcc tgctctgccc gggttcaaat atccgaaaag
     3421 gttcagaagc agtaaaaaaa gaaacaatag tgccgtcata aaagtcatcg atttccttaa
     3481 agccagaccg aaaatagaca caaaacgctg caagcagtgc aatatgtgtg ttgaaagctg
     3541 ccccgtaaaa gctatcgata aagaatcaaa gaagatcaac tataatacct gtatagaatg
     3601 tatgtgctgc catgaactct gtttgcacaa agcgattgat ttgaagaatg ataactggct
     3661 tgccgcgcta tttatgcgat tttatcgcaa gagagccgaa taataactgg tgagccagat
     3721 ctgatcgaag ataaaaaaac agtctacctg ctctatggtc cccatattgc cgaagctaaa
     3781 atccaacttg ctgaagctgg tgttggtact cttctccttc agtatcttgg catcactgct
     3841 gcctcaggtc atcctcccca tcgccagcgt gtacggagca aatgttgctg gtgaagacat
     3901 cgtgcttttc atttattata gtggatttaa cccctttgca gtctccgcca ttactgctgg
     3961 tcagctgctt ttttcagcct ggtatttaat aagggttgga cgactaaaag aatttatttc
     4021 ctgggtgact tcccaaatac cacaacaatc tacagcaact cgctggcaga aagtattgat
     4081 aatcggacta ctccttgtcg gaatactagg cttgagaagt gtcagcagct tacgaacaca
     4141 cgagcaaatg gcaccgcttg aatatgatgc aaaaatcgag atagactttg ccgaccataa
     4201 taatggtacc atttatgagt ttgccttaga cgattatcaa gtttatcatt ttgtctattc
     4261 tcttgacaca aaagatcctg taacgttgaa gcttaccact atgaatccct cgggattttt
     4321 atatactccg ggaaaaatcg attaccatgt accagggtgc agaaaaactg cagaagggcc
     4381 agttcaccgg tttcatccta gaacctggag attatcagat tgagctggat gctttaaatc
     4441 agcttggtga gctgaccatg tatatctctt ctactccacc aacaactgaa gaatatcaat
     4501 ttctagaaat tatcaaagca ttacgcgatg gcagttttac tgcagcgcat taccaggaac
     4561 caggatatga acttatctac catggagaat tgaaagatgt cggtaaccag ctgttatata
     4621 ctttgaacca gtctggtact tttcttcaaa tctcatcctt tgttgtaggg acgtatgaag
     4681 aattggatct gcgctatctg gaggagaacc gtgaatttca ccttctcgat gataaaaact
     4741 gcacgatagg gtatggtttt gatgccaaag ccaaggggga gttacgtctc tcaaccgttg
     4801 gtgcagaatt atatatctat gcaaaaaaat aagaggggtc tgcccctcta cttgtgatat
     4861 tgcttactta tacagtaaat gttaatgccg cagggttagt ttttggaaag gcggcattta
     4921 gatccgcctg cagagcagct tttactgttt caatattagc gagggcatca tagttatcac
     4981 ccaatgcgcc aatattagta agaggtactg tcacctcacc ggattcagac agtaaaacaa
     5041 gattttttcc tgtggtttct gtggcaacgt taacgctgct gcccagcgtt gttgccgcta
     5101 ctagttcggc agagtcagca tatatacctt catccgtttc attactccag cccgctccgg
     5161 acgctgtacc tccctcatta gcagtaatac taattggaac tgcactttgt gtggcccaaa
     5221 aatttgggtc agtataaccc agaacaccaa gcgcttccgg atctgaatta gaaaggtcta
     5281 cacaaccagt tagaaaaaaa agtactgcga gcaataaaag agtaattttt ttcagttgcc
     5341 ccaatttcat atttcttccc cccgtttttt tgaatgccaa tcaaaaaagc tgggctgctt
     5401 tataacactg cagattgttt accagatgcg tgtatgcttg tccatccggc ctatgtactt
     5461 cgttttgttt tgtttgagat tatgagatga tcttcaaata ttacgcagtg tagcataccc
     5521 cttctatttt agtcccaatt ttcacaaaat acaatatatt attccttaat attttctttt
     5581 cccctccgca gctctatttt gggaactaaa tataattggt taatatgaga tttgtgttga
     5641 agaaggaata acaacaaggc agtttctaat tgacggtaag ttttatga
//
LOCUS       DASRMS010000213         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_535312, whole genome shotgun sequence.
ACCESSION   DASRMS010000213 DASRMS010000000
VERSION     DASRMS010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535312"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..206
                     /locus_tag="VFC74_10270"
     CDS             <1..206
                     /locus_tag="VFC74_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25751.1"
                     /translation="YLRSSLVELGAPGEMVSYFMGHWEHGEEPHQKLSSISPIDIAIQ
                     LAPYLNTIEKQIGWTVQGGLAHV"
     gene            199..>3205
                     /locus_tag="VFC74_10275"
     CDS             199..>3205
                     /locus_tag="VFC74_10275"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HZK25752.1"
                     /translation="MCEVKYWPGWSFIKTQDGILGHNRRRRQQVENQLNQQKKLLQLL
                     DARAPGCALGAENIQISNLAIDEVNAWIDEQDSANRATTHLRRFLRAGLAKGAQELGW
                     HVTLPPTELTIISEKNPITPDGFIQLAQLRKQICWFKERLVNPPSDPSISLIYGQLLF
                     SATVFGGLADPKAMLQLPYAKEKIRHHKDYVWVDLFTDDYPKHAQVERGLRRWFPDPV
                     SLILLTRLPELPQGYEDKNKLRRWLNQALAQYICSLLGDVLGRATYKEQQVLIRHWRA
                     TVGVYHHTILPPYLVRHCTGESSSTAWPELVWLRVLKQQALIQSAADEIAGLTMDEPE
                     PIIAPVALTINDSKKNLIFTAVRAALYKSSQTTEPSLKTLLERLGKLKTEAEQASPLL
                     YCLIDWVKKRLRSTEIRRSTAYQQLTSIGKNILSTFDETDLPTLAAEDFQAGYEAMLE
                     NTPSAITRQNKADLLRRFHAHASNILQLAPINLSYADEGFFALPDANIITETEYQLLY
                     QFLKSQKDTSLIAHTQLIVLILGYRCGLRISEVRHLQLVDLQYPGTLEILSTERLKQL
                     ERYAATLLIRGDQFNKLKSTSSRRQLPLNILMSSEERVELLSYHQAQRVKVGKRKVQG
                     KFIFSEDNGKTPMDFTELQRGLHEQMRLLTGDPDLRFHHLRHSFASHALQASQNLTEP
                     LQLPAHWKNHMLQGVSRKHLHQISSYVGHSSGNVTLQHYTHNLDCIVRDLLWPHSFDH
                     RKTGHGELQRYRGSLHTALCLQSLLDISADNLRQHNRQYDGKILGWLPQRLKLQPASD
                     PNQWQFYSENLARLVAGTSNEPRDLRDLNFENWCGFLERWSQLQTGDELIEMYKLDET
                     NTRAQVSLLSDLLKVQTRFDNSRLKQPRKIFKAATTASSHYFIVRPPRYKLERDIAAQ
                     IYKNSLRLLQSTQNGDMAKTALEYFYDNYRHWDKSVTCRHDPSNLPKLQALEQFLVRV
                     LPNDKVIVEQERNPAGHRYPMIGKLQIVTV"
BASE COUNT          940 a          733 c          754 g          778 t
ORIGIN      
        1 actacctgcg tagcagtctg gtcgaactcg gtgcacctgg cgaaatggtt tcatatttta
       61 tgggccattg ggaacacggt gaggagccgc atcaaaaact atccagcatc agcccaatag
      121 acatagcaat tcagttagcg ccttatctca atactattga aaaacagatt ggctggacgg
      181 tacagggagg gttggcccat gtgtgaggtg aaatactggc cgggttggtc ttttataaaa
      241 actcaagacg gcattttggg ccataaccgt cgacgcaggc aacaagtcga aaatcagctg
      301 aatcagcaga aaaaactact gcagctattg gatgcccgtg ctccaggctg tgcgctaggt
      361 gcagaaaata ttcaaatttc taatctagct atagatgagg tcaacgcatg gattgatgag
      421 caggatagcg ctaatcgtgc aaccacccac ctgcgtcgat ttttgcgggc tggactggct
      481 aaaggagcgc aagagcttgg ctggcacgtt acgctaccac caactgagct gactatcatc
      541 agcgagaaaa accccattac tcccgatggg tttattcagt tagctcaatt gcgtaagcaa
      601 atttgctggt ttaaggagcg attagtaaac ccgcctagtg atccgagcat tagtcttatc
      661 tatgggcaac tgctatttag cgccacagtt ttcggcggtc tagcagaccc gaaagcaatg
      721 cttcaacttc catatgccaa agagaaaatt cgtcaccata aggactatgt ttgggtcgat
      781 ctatttacgg atgactaccc aaaacacgcc caggtagagc gcggtttacg cagatggttt
      841 cctgaccctg tatcactcat tttactgact cgactgcctg aattaccaca agggtatgag
      901 gataaaaaca aattaaggcg ttggctgaat caagctttgg cacagtatat ctgctcattg
      961 ttaggtgatg ttctaggtcg ggcaacatat aaagaacaac aagtcctgat aagacactgg
     1021 cgcgctactg tcggtgtgta ccaccatacg attttaccgc catatctcgt gcggcactgc
     1081 actggtgaaa gcagctcaac cgcttggcca gagttggtgt ggctgcgagt attgaagcaa
     1141 caggctctaa tccaatcggc tgctgatgaa attgctgggc tgaccatgga tgaacctgaa
     1201 ccaataattg cgccagtggc attaaccatt aacgatagta aaaagaatct gatttttaca
     1261 gctgtgcgtg ctgctttata taaaagcagt caaacgacag agccaagttt aaaaaccttg
     1321 ctagaacgcc tgggcaaatt aaaaacagaa gccgagcaag cgagtccgtt gctgtactgc
     1381 ctgattgatt gggtcaagaa acgcctgaga agcacagaga ttcggcgtag tactgcctac
     1441 caacaattaa ccagcatcgg gaaaaatatt cttagtactt ttgatgaaac agacttacca
     1501 acactggcag cagaggattt tcaggctggc tacgaagcca tgctagaaaa tacaccctca
     1561 gcaattacgc gacaaaataa agcagatttg ttgcgcaggt ttcatgctca cgcaagcaat
     1621 atcctgcagc ttgcccccat aaatctcagc tacgcagatg aaggtttttt cgcattgccg
     1681 gacgccaata tcatcacaga aactgaatac caactgctgt atcagtttct aaagtcacaa
     1741 aaagacacat cactcattgc acacacgcaa ctgattgtgc tgattttagg ttataggtgt
     1801 ggattgcgta ttagtgaagt aaggcattta cagttagtcg atctgcagta ccctgggacg
     1861 ctggagatat tatctacaga gcggttaaaa cagctcgagc ggtatgccgc aactttatta
     1921 attcgcggtg atcagtttaa caaactaaaa agtacgagca gccggcgtca gctgccatta
     1981 aacatactca tgagcagcga ggagcgagtt gaactgctga gctatcatca agcccagcgc
     2041 gttaaagttg gtaaaagaaa ggtgcaaggg aaatttattt tctctgagga taatggcaag
     2101 acgccgatgg atttcactga gttgcaacgt ggcctacacg aacaaatgcg gctgttaact
     2161 ggtgatccag acctgcgatt tcatcacctg cgccacagct ttgccagcca tgcgcttcag
     2221 gcgagtcaaa acttaactga gccattacaa cttccagctc actggaaaaa ccatatgctg
     2281 caaggtgtta gcaggaaaca cttacatcaa atatctagct atgttggcca ctctagcggg
     2341 aatgtgacat tgcagcacta cactcataac ctcgactgta ttgtgcgaga tctgttgtgg
     2401 ccgcacagtt ttgaccatag aaaaaccggg catggtgaac tgcaacgata tagaggctct
     2461 ctgcataccg cgctctgcct gcaaagtttg ctagatatca gcgctgacaa cctacggcaa
     2521 cataatcgcc aatacgacgg aaagatttta ggttggctgc cgcagcggct taaactgcag
     2581 cccgctagtg atcctaacca atggcaattt tatagcgaaa atctggcaag actggtagcc
     2641 ggcacatcaa atgagcccag agatctgcgt gatttaaatt tcgagaattg gtgtggtttt
     2701 ctagagcgct ggagccagtt acagactggt gatgagctga ttgaaatgta taagctggac
     2761 gaaacaaaca cacgagctca ggtctcactg ctttcagatt tactgaaggt gcagactcgg
     2821 tttgataatt cgcggctcaa acaaccccgt aagatattta aggcagcaac cacagcgtca
     2881 agccattatt ttatcgttcg gccacccagg tataagctcg agcgcgatat tgctgctcaa
     2941 atctacaaaa atagtttacg cctgctgcaa tctactcaga acggagatat ggctaaaaca
     3001 gcactagagt atttttatga caattaccga cactgggata agagcgtcac ttgccgccat
     3061 gacccaagca acctgcctaa actacaagct ctggagcagt ttctagtcag ggtgttgcct
     3121 aatgataaag tcatagtaga gcaagaacgt aaccccgctg gtcacagata tccgatgatt
     3181 gggaagttgc agatagttac agtag
//
LOCUS       DASRMS010000214         1049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_398164, whole genome shotgun sequence.
ACCESSION   DASRMS010000214 DASRMS010000000
VERSION     DASRMS010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398164"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..188)
                     /locus_tag="VFC74_10280"
     CDS             complement(<1..188)
                     /locus_tag="VFC74_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25753.1"
                     /translation="MKTLTAIPLSELIKKAGSKRKVAQLAGIQNLGSLSHESVLIGDR
                     MYRPSISTADWAKVNKELV"
     gene            complement(185..688)
                     /locus_tag="VFC74_10285"
     CDS             complement(185..688)
                     /locus_tag="VFC74_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25754.1"
                     /translation="MNEINEEIKLTIDTMQCDIDDGAHSELQSHLYSLLEIKRNELQQ
                     RLVERSWAEPVKPLTTEELMDENEKLRMQLSACGVAALSNTANSAEKMRCISSEYKSA
                     SWQSVCDAVDREMAFREQCEKLFDALQIITETAQRCDGWSSFPIHDIKLAEDAMDNFK
                     VQMSKLK"
BASE COUNT          227 a          271 c          214 g          337 t
ORIGIN      
        1 accaattctt tatttacctt ggcccaatct gctgtactga tgctgggccg atacatccta
       61 tcgccgatca acacagattc gtgcgacagg ctcccaaggt tctgaatgcc tgctagctgt
      121 gccactttac gcttgctgcc tgcttttttt attagctcgc ttagtgggat tgcggttagg
      181 gttttcattt caatttactc atttgaactt taaagttatc catagcgtct tccgcaagct
      241 taatatcgtg tattgggaat gaagaccagc catcacacct ttgtgcagtt tctgtgatga
      301 tttgtagcgc atcaaacagc ttttcacact gctcacgaaa tgccatttca cgatctacag
      361 catcgcacac gctttgccat gaggcgcttt tgtattcaga gcttatgcag cgcatttttt
      421 cggcgctatt agcggtattg gatagggctg caactccaca cgctgacagc tgcattcgaa
      481 gcttctcgtt ctcatccatc agctcttcgg tggtcaacgg cttaacaggc tctgcccacg
      541 aacgctcaac caatcgctgc tgcagctcgt tgcgcttgat ttctagcagg ctgtacaggt
      601 gactctgcag ctcactatgc gccccgtcgt cgatgtcgca ctgcattgta tctatcgtta
      661 gtttgatttc ttcgtttatt tcgttcatgt tcattcctat ttatctgatt ctgttgcgtc
      721 agataaatta tctataacct tcccaacaga agtagcagtg taaggctcat ctttatctgt
      781 cagcgctcct ccgagcgtca cagcgtctgc cacaacagcg ataggtgttt caattacaac
      841 tccaacagct gcttttgtta cgcttttaac attccaaaca tattaacccc tctatcgtga
      901 tgagacccac cgcgtaccag ttggcagagg cacgggtcgt gttgaactat tagctgcacg
      961 gatatctcga cagctgtcct gtttctgcga aatactctaa ttcttttgat agtattttcg
     1021 cctgatctct agttagatgc attctggta
//
LOCUS       DASRMS010000215        10865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_126744, whole genome shotgun sequence.
ACCESSION   DASRMS010000215 DASRMS010000000
VERSION     DASRMS010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 10865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10865
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126744"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(376..1623)
                     /locus_tag="VFC74_10290"
     CDS             complement(376..1623)
                     /locus_tag="VFC74_10290"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25755.1"
                     /translation="MTNTAQVRREAKISRFVAAETLGTPDRVPTFTVPTFFPTKMTGM
                     TVKDAFYDQEKLLEAYHKYLEVYDPDMYFGPNVAVGVSGAALDILETKQIMWPGNGVD
                     DNASFQLVEGEYMTQAEYDQFLNDSSDFNLRVYLPRVHNALAGFGKFPPLLSIANGYN
                     SPFIGAFADPKILQSLKKIVEAAEIQAKWMENYAAFEKEAAEHGHVNLMGGIALAPFD
                     LISDMLRGMKGTMFDMFQEPDKLLAAQDKFLPLAINSAIGMTKAANNPRVFIPLHRGA
                     DGFMSNKQFEKFYWPHLKKIIEAITDAGCIAYPFFEGTYDERLEYLQELPSGKTVAWF
                     DRSDMVRVKEVLGGKLCIAGGMPITLLQASTVAGVKEYTKDMIDTVGRDGGFIMTPSS
                     VLDEAEPALVKAWHDAVNEFGVY"
     gene            complement(1630..2262)
                     /locus_tag="VFC74_10295"
     CDS             complement(1630..2262)
                     /locus_tag="VFC74_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356206.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25756.1"
                     /translation="MSGKLFEAIVDMRDKDALQLTQEMLDNGVNPRDILAECQEAMAE
                     VGRLFEAKEYFLPELMMSGEMLKQISEILKPLMEGSADEGEKLGRVVLGTVKGDIHDI
                     GKDIVAFMLEINGFEVFDVGIDQPPQVFVDKIKEVNPHVVGLSGFLTVAFNSMRDTVE
                     MIKEAGLREQVKIMIGGGTVTEDLVGYTGADAFGQDAMTAVQLSKTWVKE"
     gene            complement(2499..3737)
                     /locus_tag="VFC74_10300"
     CDS             complement(2499..3737)
                     /locus_tag="VFC74_10300"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25757.1"
                     /translation="MEKSAQARREAKIKRFVTAETLGVPDRVPTFTVSGFFPTKLTGM
                     TVKDAFYDQEKWLEANRKYLEVYDPDMYFAPSSASGAVHDILETTQIMWPGNGVDDNA
                     SFQFVEGEYMTQAEYDMFLNDPSDFNLRVYLPRIHKALAGFANLPPLLSIARGYNSPF
                     LGAFANPELVQSLEKLVEAAKVQAQWSKAYAGFEQEASANGHVNLIGGITLAPFDSIS
                     DTLRGMKGTMFDMFQEPDKLLAAQDKLLPLLINAAIGMTKATKNPRVFIPLHRGADGF
                     MSVKQFEKFYWPYLKKLIEAITDAGCIAYPFFEGTYDARLEYLQELPSGKTVAWFDRS
                     DMVRVKEVLGGKICIAGGMPITLLQTSTVAGVKEYTKQMIEAVGQDGGFIMTPSCALD
                     EAEPELLKAWQDAVNEFGVY"
     gene            complement(3745..4377)
                     /locus_tag="VFC74_10305"
     CDS             complement(3745..4377)
                     /locus_tag="VFC74_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014356206.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25758.1"
                     /translation="MSGKLFEAIVDMRDKDALQLTQEMLANGVNPRDILAECQEAMAE
                     VGRLFEAKEYFLPELMMSGEMLKQISEILKPLMEGSADEGEKLGRVVLGTVKGDIHDI
                     GKDIVGFMLEINGFEVFDVGIDQPPQVFVDKIKEVNPQVVGLSGFLTVAFNSMRDTVD
                     MIKEAGFREQVKIMIGGGTVTEDVVGYTGSDAFGSDAMAAVQLAKAWVKE"
     gene            complement(4439..5815)
                     /locus_tag="VFC74_10310"
     CDS             complement(4439..5815)
                     /locus_tag="VFC74_10310"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HZK25759.1"
                     /translation="MEKKTPSIGLVSSTYGLAWLGFTLLTSMNTTYYIYFLTEVVKFT
                     PAAMGALLSGVRLIGLISSLVVGIVIEKTNFRWGKYRSWLLFAAPGASIFYALMFTNP
                     NLSPTMKMVYLAFVYVMMQNFLNLLSASSMSFIPIIGQSPTARVLLSSRRAQFMTGGR
                     IFFSIISMPVILFFNRGVEANAFGYFAVTALFGLVCIGGHLMFFNMTKEYDKPQPKGT
                     ASGPTLSLKDMLEQLTKNPPLLLLIVSNSAMMTAFMTMTALAAYYFSYVVNNIVMIAT
                     YMTVISVTQLLGALATPNICCKIDKRSAYIFGLLMVSTGHLLAWAFAKTALLFIIING
                     IGYFGYAIATSTLGAMFSDTCDYAEWKTGKSARGLIMSMSGIPPNIGIFLSSLVLSLG
                     FTAIDFVAGMESTPALVSGINVLINLVPSGLMLIGAVAIFFYSLTNTRVQQLQQECAA
                     RQAAATKA"
     gene            complement(6316..6786)
                     /locus_tag="VFC74_10315"
     CDS             complement(6316..6786)
                     /locus_tag="VFC74_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition protein"
                     /protein_id="HZK25760.1"
                     /translation="MVEILREALSTSLVTICRLAIILMPLMIVLEYAKHFNVLEKLTK
                     YLGWLPRGLGISEQAVFPLLVGMIVGITYGAAVIIDYARQGTISKRDMTLVGIFLAVN
                     HSVVEDNLLFASLGASLLVLLPLRFLFAFLLTRAVAMIAKVDEAELETEAAPQK"
     gene            complement(6786..7223)
                     /locus_tag="VFC74_10320"
     CDS             complement(6786..7223)
                     /locus_tag="VFC74_10320"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HZK25761.1"
                     /translation="MDYWKPARQGLRKALRTTLLLAKIIIPVTFVIVSLKRIGIIGQL
                     ADLLAPFLKILGLPGEAALPLFLGFFVNIYAAIGAISLLALTGWQITIIAVMILTCHS
                     LLMETPVLKLSGVHPAAAVLIRFGSAMILGFALHLLYQVVGVY"
     gene            complement(7424..7726)
                     /locus_tag="VFC74_10325"
     CDS             complement(7424..7726)
                     /locus_tag="VFC74_10325"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HZK25762.1"
                     /translation="MFFPMLYFHRWQGTFSKREIRQNRTNIVKIKEVLGDHMCVTGDM
                     PPAILELGTPDDNYNYARKLIDLFGKSGFIMSSGCSVPPHAPLENIKAVIAATLEG"
     gene            complement(7665..9002)
                     /locus_tag="VFC74_10330"
     CDS             complement(7665..9002)
                     /locus_tag="VFC74_10330"
                     /EC_number="4.1.1.112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005805510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxaloacetate decarboxylase subunit alpha"
                     /protein_id="HZK25763.1"
                     /translation="MTKLKVTETSLRDGHQSLIATKLTTDEILPILNEMDNAGFYSLE
                     VWGGATFDACLRYLNEDPWTRLRKIKANVKNTQLQMLSRGQNLLGYKNYPDDIVDRFL
                     KNAIFEGIEIIRTFDALNDIRNLKKSIEVIKREGAHCQCAISYTTSPVHSTEYYLELI
                     KAFANEGADSICIKDMSGILLPYDAYHLVKSIKEVTRLPLEFHTHSTSGVAQMSMLKA
                     IEAGVDIVDTAISPLSSGTSQPATESIALSLLGTKYDPQLNLDSLNRTADYFKEVMKK
                     YQENDALTAKVLMTEPKILQYQIPGGMLSNLIFQMKELNAADKYEAVLAEVPKVRADL
                     GYPPLVTPMSQMVGAQAVFNVISEGRYKVIPKEIKNYVRGMYGRSAAPIADEIKRKII
                     GDEAVITVRPADLLTDEYDKLKAEIGELAKSEQDVLSYALFPQVARDFFEKRN"
     gene            complement(9170..10147)
                     /locus_tag="VFC74_10335"
     CDS             complement(9170..10147)
                     /locus_tag="VFC74_10335"
                     /inference="COORDINATES: protein motif:HMM:NF022986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3231 family protein"
                     /protein_id="HZK25764.1"
                     /translation="MKITALLTKKPEKKANDVDIREAFTLWDILNSKYTTLEQLLMCD
                     SFTEDAELKLLLKKIELEIEENITILQKQMKKYNIISPNKNRAAVVNYTNQDLMTDEA
                     IAMQMLLYEQEHVENLVLAVYSIITNDNLRKVIIEMLLRTLKSTDMLMKQAAIRGWVG
                     IPPAYQHLPADTTEKIHCGEAGCLWDMLTYRYDTLHSTEIMTNIVKDVDLKVIFTAGI
                     QLLKDQIKQLEKELVYFGITLPKRPGEITAALDNKDLWEDSHIYRMTLMGMQGAGTLH
                     VRAFKKLSSSHRLRNYMMKLLWQEVEKIDDLIRYGKVKGWLQQVPRYGP"
     gene            complement(10238..10621)
                     /locus_tag="VFC74_10340"
     CDS             complement(10238..10621)
                     /locus_tag="VFC74_10340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003373666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25765.1"
                     /translation="MNRKMLAAVSAISTEHQVILRGYVQLSGGVHGFLFGHYCKNTLF
                     YKDLFKVTRDVFRVNRLAKQNLRQIKKLLKKYGYRRIWAKGVFSIYGDLRPLAVAAGL
                     GQWENGLVANAKYGSDFLISGIFFK"
BASE COUNT         3123 a         2566 c         2019 g         3157 t
ORIGIN      
        1 gttaaaatta tacctaaatc agtcattatt tctctcctag cattcttagc aattaagtta
       61 agcttattct atcataatat cacaaaaaaa ctctactaaa ttcagctttt tagttacctg
      121 tgacggcttc gatgaattat agcttattta atttcttaat tgacataatt tctctgtaac
      181 gaaaaactgc ccagctacat aagacagtct agcagtaaat tattaagtta aacttaagaa
      241 tgtcttaaat ttttgctttt ctttagtttc tacaagccgc tgcgctttcg gtacgtggat
      301 aaatccaatt aaactttgtc agtaaaaaca acagtgtgac aggcagcaaa cctgtcacac
      361 tgttttaact aaatactaat aaacgccaaa ctcattgaca gcatcatgcc atgctttaac
      421 taatgctggt tcggcttcat ctagaacact gctcggcgtc ataataaaac cgccgtctcg
      481 acccacagtg tcaatcatat ccttggtata ctctttaaca cctgctacag tggaagcctg
      541 caacaaggtg attggcatgc caccggcaat acaaagctta ccgcctaata cttctttgac
      601 gcgcaccata tctgaacgat caaaccaagc aacagtttta ccagaaggca attcctgcag
      661 atattctaac cgctcatcat aagttccttc aaaaaaggga taagcaatac aaccggcgtc
      721 ggtaatcgct tcgatgattt ttttcaggtg tggccagtaa aacttttcaa actgtttgtt
      781 tgacataaag ccatcggcac cacggtgtag agggataaag acacgcggat tgttcgccgc
      841 tttagtcatg ccgatggctg agttaatcgc cagcggcaaa aatttgtctt gcgcggctaa
      901 tagcttatcc ggttcctgga acatatcaaa catggtaccc ttcatacccc gcagcatatc
      961 ggaaattaaa tcaaagggag ccagggcaat accgcccatt aaatttacat gcccatgttc
     1021 tgctgcttcc ttttcaaaag cagcatagtt ttccatccac ttggcctgaa tttcagctgc
     1081 ctctactatt tttttcagcg actgcaatat tttaggatca gcgaaggcac cgataaatgg
     1141 cgaattataa ccgttagcaa tcgagagcag gggtggaaac ttaccaaagc ctgctagggc
     1201 attgtgaaca cgaggcaaat aaacacgcag gttaaaatca gaggaatcat ttaaaaactg
     1261 gtcatactct gcttgtgtca tatactcgcc ttcaacaagc tggaaggaag cgttatcatc
     1321 gacaccatta ccaggccaca taatctgctt tgtttccaga atatcaagcg ccgccccact
     1381 tacaccaacg gcaacattgg gtccaaaata catatcgggg tcataaacct caagatattt
     1441 atggtatgct tctaacaatt tttcctgatc ataaaaggca tctttcacgg tcatacccgt
     1501 cattttagtg gggaaaaagg tcggtactgt aaaagtcggc acacgatccg gtgttcccag
     1561 cgtttccgca gcaacaaacc tactgatctt ggcttcacgt cgtacttgtg cggtatttgt
     1621 cattcagact cactccttta cccaagtttt agacagctgg acagctgtca tggcatcctg
     1681 tccaaaggca tcagcgccag tgtaacctac gagatcttcg gtaacggtac cgccaccgat
     1741 cataatttta acttgctcac gtaaaccagc ttctttaatc atttctacgg tgtctcgcat
     1801 ggagttaaag gctacggtta aaaagccgct caaaccgaca acatgaggat taacttcttt
     1861 gatcttatct acgaagacct gaggaggctg atcaataccc acatcaaaga cctcaaagcc
     1921 gttaatctcc aacataaaag ccacaatatc tttaccgata tcatgaatat cacctttaac
     1981 tgtacctaaa accactctgc cgagtttctc accctcatca gcgctgcctt ccattaaagg
     2041 ttttaggatc tctgatattt gctttaacat ttctccactc atcattaatt ccggtagaaa
     2101 atattccttt gcttcaaata gtcggcctac ctcagccatc gcctcttgac actccgccag
     2161 gatgtcacgt gggttaaccc cattatccag catttcctgg gtaagctgca gagcatcctt
     2221 gtccctcatg tcaacaatcg cctcgaatag ttttcctgac attaacaatc ctccttttct
     2281 tttccctcat cgtttcaacc ttgctccaga cacctaataa aggaacctat ttgccacttt
     2341 tataatattc tgactatttc tttcatatac caccgcccgc gcaattaaag tgtttgtggt
     2401 gtttttcgtt gtttttggca ttcttcttcc ctgcgctgta ccagaagatc tgaaaaaaaa
     2461 cagtgtggca ggaaataaac ctgccacact gactcatact aataaacgcc aaactcattg
     2521 acagcatctt gccatgcttt aagtaactct ggttcggctt catctaaagc gcaacttggt
     2581 gtcataataa agccaccgtc ttgacccaca gcttcaatca tctgcttggt atactctttc
     2641 acacctgcta ccgttgaagt ctgcagcagt gtaatcggca taccaccggc aatacagatt
     2701 ttaccgccca atacttcttt gacacgcacc atatcggaac ggtcaaacca agcaacagtt
     2761 ttaccagacg gcaattcctg cagatattct agccgcgcat cataggtgcc ttcaaagaag
     2821 ggataagcaa tacaaccagc atcggtaatc gcttcaatca gttttttcag gtatggccag
     2881 tagaacttct caaactgttt tactgacata aagccatcgg caccacggtg gagggggata
     2941 aagacacgtg gatttttagt cgctttagtc atgccaatag cggcgttaat cagcagcggt
     3001 aatagtttat cttgcgctgc caagagttta tctggttcct ggaacatatc aaacatggta
     3061 cccttcatgc cacgcagtgt atcagaaata ctatcaaacg gggctagggt aataccgcca
     3121 atcaagttga catggccatt ggcactggct tcttgttcaa aaccagcgta agccttactc
     3181 cactgggctt gaactttggc ggcttctact aatttttcca gcgactgcac taattctgga
     3241 ttagcaaagg cgcccaggaa gggagaatta taaccgcgag caatagatag cagcggcggt
     3301 aaattggcaa aacccgctaa agccttatgg attcggggca ggtaaacacg taagttaaaa
     3361 tcagaaggat catttaaaaa catatcatac tctgcctgtg tcatatactc accttcgaca
     3421 aactggaacg aagcattatc atccaccccg ttaccaggcc acataatctg tgttgtttcc
     3481 aagatatcat gaaccgctcc acttgcactg ctgggcgcaa aatacatatc ggggtcataa
     3541 acttcaagat atttacggtt tgcttctaac catttctcct gatcatagaa ggcatctttt
     3601 acggtcatgc ccgtcaattt agtggggaag aaaccggata cggtgaaagt cggcacacga
     3661 tctggtactc ctagggtttc tgcagtaaca aaccttttga ttttggcttc acgacgtgct
     3721 tgtgctgatt tttccattcc ttactcactc ctttacccat gctttggcta gctggacagc
     3781 tgccatggca tctgaaccga aagcatcaga gccagtgtaa ccaacaacat cttcagtaac
     3841 ggtaccgcca ccaatcataa ttttaacttg ctcccggaag ccagcttctt ttatcatgtc
     3901 aacggtgtct cgcatggagt taaaggctac agttaaaaag ccgcttaagc ccacaacttg
     3961 gggattaact tctttgatct tatccacgaa gacctgggga ggctgatcaa tacccacatc
     4021 aaagacttcg aagccgttaa tctctaacat aaagcccaca atatctttac caatatcgtg
     4081 aatatcacct ttaactgtac ccaaaaccac tctgccgagt ttctcaccct catcagcgct
     4141 gccttccatt aaaggtttta ggatctccga tatttgcttt aacatttctc cactcatcat
     4201 taattccggc agaaaatatt cctttgcttc aaagagtcgg cctacctcag ccatcgcctc
     4261 ttgacactcc gccaggatgt cacgtgggtt aactccatta gccagcattt cctgcgtaag
     4321 ctgcagagca tccttgtccc tcatgtccac aatcgcctca aatagttttc ctgacatcga
     4381 caatcctcct ttttttctta acaataaatt gaatcaacca cagagatact atttaccttt
     4441 aagctttggt tgcagcagcc tgccgagcag cacactcctg ctgtagctgc tgtactctag
     4501 tgttagtcag gctgtagaag aagatcgcca cagcacctat cagcattaag ccgctgggaa
     4561 ctaagttaat cagcacatta attccgctga ccagtgctgg cgttgattcc atcccggcca
     4621 caaaatcaat ggcggtaaaa ccaaggctta agactaggga actcaagaag ataccaatat
     4681 tagggggaat gccgctcatc gacatgatca ggcctctagc acttttgcct gttttccatt
     4741 cggcatagtc acaggtatca gaaaacattg cgccgagagt actagtagca atggcgtacc
     4801 cgaaataccc tatcccgtta atgataataa agagcagtgc cgtcttagca aaggcccagg
     4861 caagcaggtg acctgttgaa accatgagta agccaaaaat ataagcagat cttttatcga
     4921 ttttgcagca gatgttaggt gtagctaaag ctcccaacag ctgagtgaca cttatgactg
     4981 tcatataagt cgcaatcatc acgatattat taacaacata actgaagtag taagcagcca
     5041 gtgccgtcat cgtcataaag gctgtcatca tcgctgaatt tgaaacgatt aataacaaca
     5101 gtggcgggtt ttttgtcagc tgctcaagca tatcctttaa cgatagtgta ggaccggatg
     5161 ctgtaccttt gggctgaggc ttgtcatatt cttttgtcat gttaaagaac atcagatggc
     5221 caccaatgca caccagacca aaaagcgctg taacagcaaa gtaaccaaag gcattagcct
     5281 caacgccgcg gttgaagaaa aggattacag gcatactgat aatactaaag aagatccggc
     5341 cgcccgtcat aaattgagca cgccgcgaag aaagcaagac tctcgctgtt ggtgactgac
     5401 cgataatcgg gataaaagac atagaagatg cactaagcaa attaaggaaa ttctgcatca
     5461 tcacataaac aaaagccaga tataccatct tcatcgtagg tgataaatta gggttagtga
     5521 acatcaaagc atagaagatc gaagcgccgg gggcggcaaa taacagccaa gaccggtatt
     5581 tgccccacct aaagtttgtt ttttctatca cgatgcccac gaccaatgaa gatattagtc
     5641 caatcagtcg caccccactc agtaatgcgc ccatcgcggc tggggtaaac ttaacaacct
     5701 ctgttaaaaa atagatataa taggtggtat tcattgatgt gagtaatgta aaaccaagcc
     5761 aagccagacc ataagtactg gatactaaac caatgctggg agttttcttt tccattgctt
     5821 ttcctccttg tttctgaact tacttctttg ctgcgtgata aataaacaag gccactttta
     5881 catgcactgc accggacaag ctatttaaaa tattttgact atttagtcca ttgtccgaca
     5941 taattgatga aaagctgttt taggctgact cttctctagg agtcagctag gcataggttt
     6001 tacacctccc attgtgaaga taatactaat tatattacgc aataattaaa tattcaatac
     6061 cttttttatt tttgcactat ttagcacagc attatctgat taaaagggcg caggactgac
     6121 aatctttgcc cggtctgctt cttctcacgt acagaaaaag atgtgtaact agaaacagtc
     6181 catgtcacac atcttcttga gcattgatga gggcagcaaa ctcgtttaac aactgctaca
     6241 gtgatacttg cgccagtgac tcttcccgag ccgaggtaac agagcttgat aggaacgacc
     6301 cgtaccacaa actcactact tttgcggagc tgcctcagtc tccagctccg cttcatccac
     6361 tttggcaatc atggccactg ccctggtgag cagaaaggca aacagaaagc gcagcggtaa
     6421 aagtaccaat agactagctc ccaaggaggc aaaaagcaaa ttatcttcaa ctacactatg
     6481 attaacggcc aaaaagatac cgaccagcgt catatccctt ttgctgatcg taccctgtcg
     6541 ggcgtaatca ataatcactg cagctccgta ggtaataccg acaatcatac caacaagcaa
     6601 aggaaacacg gcctgctctg agatacccaa accacgaggc agccagccca aatatttcgt
     6661 tagtttttct aagacattaa aatgtttggc atactctaaa acaatcatca agggcatcaa
     6721 aataatagct aaacggcaga ttgtgaccag acttgtactt aaggcctcac gcagtatttc
     6781 taccatcaat aaacccccac aacttgatat agcagatgta aagcaaatcc taaaatcatc
     6841 gcgctgccaa acctaatcag cactgccgca gctgggtgca ctcctgacaa ttttaatact
     6901 ggtgtctcca ttagtagtga gtgacaagtc aaaatcatca ccgcaataat ggttatttgc
     6961 cagcctgtca gtgccaggag agatatggca ccaatcgcag cataaatatt gacaaagaag
     7021 cctaaaaaga gaggcaacgc cgcttctccc ggtagcccga gtatttttaa aaatggtgct
     7081 aataagtccg caagctgccc aataatccca atcctcttta aggacactat cacaaacgtt
     7141 acagggataa taatttttgc taatagtaat gtcgtccgca aagccttacg taaaccttgt
     7201 ctagcaggtt tccaatagtc caagcaaatt cctcctagcg tagagtcagt aggtgcttat
     7261 gctgttattc tcttactttg gcctgattcc ttttgttagt agatactact tcccagtgtc
     7321 tcacctccta tgcttaattc gctctatttt ttaacagaaa aaaacggcgc acctttcaag
     7381 ttggcgtcgt tctgtaattt atggtcttag gctgtcttag gcgttagcct tctagtgtgg
     7441 ctgcaataac agccttaatg ttctcgagtg gggcatgcgg cggcaccgaa catcctgatg
     7501 acattataaa accactcttg ccaaatagat ctattaattt acgggcatag ttgtaattat
     7561 catccggtgt gcctaattcc aggatagcag gcggcatatc ccccgtcaca cacatgtgat
     7621 cacctaaaac ttcttttatt tttacaatat tagtcctgtt ttgcctaatt tctcttttcg
     7681 aaaaagtccc ttgccacctg tggaaataaa gcataggaaa gaacatcctg ttcagatttt
     7741 gccagctcac ctatttcagc ttttaattta tcatattcat ccgtcaataa atcagcaggt
     7801 ctaaccgtga ttactgcctc atcgccaatt atttttctct ttatttcatc agcaataggt
     7861 gctgctgatc ttccgtacat tccccgcaca taattcttaa tctctttagg aataacttta
     7921 tatctgccct ctgagataac gttaaagacg gcttgtgctc ctaccatttg gctcatgggc
     7981 gtcactagcg gaggatagcc tagatctgct ctgaccttag gtacttccgc gagtactgcc
     8041 tcatatttgt ccgcggcatt taattctttc atttggaaga ttagatttga cagcatgccg
     8101 ccaggtattt ggtactgcaa tattttaggc tccgtcatca gtactttggc cgtcagagca
     8161 tcattttctt ggtacttttt cataacttcc ttaaaataat cagctgttct atttaggctg
     8221 tctaaattaa gctgtggatc atattttgta cccagcaaac ttagggcaat tgattcagtt
     8281 gctggctgtg atgtgccgga tgagagagga gatattgcgg tatctactat atctacgcca
     8341 gcttcaatgg cctttaacat cgacatctga gctaccccac ttgtagagtg cgtatggaac
     8401 tctaggggaa gcctggtaac ttctttaatc gattttacca gatgataagc atcataaggc
     8461 aatagtatac cggacatatc cttgatacaa atcgaatctg ctccttcatt tgcaaatgct
     8521 ttgattaatt ctaaataata ttctgtacta tgaactggac ttgtggtata ggagatggcg
     8581 cattgacaat gagcaccttc tcttttaatg acttcaattg attttttcag atttctaatg
     8641 tcattgaggg catcaaatgt tcttatgatc tcaattcctt caaaaatggc atttttaagg
     8701 aatctatcta caatgtcatc aggataattt ttatagccta aaaggttttg ccccctagag
     8761 agcatctgca gctgcgtatt tttaacatta gctttaattt tcctcagcct tgtccaggga
     8821 tcttcattta agtatcttaa acaagcatcg aaagttgctc ctccccatac ttctaacgaa
     8881 taaaaacctg cattatccat ttcatttaat attggcaata tctcatctgt tgttagctta
     8941 gttgctatta aagattggtg tccatctctt aatgatgttt ccgtaacttt taattttgtc
     9001 atgcctgccc ccgcatagtt tttactatgt tttcagtgtc ctcattggat tacaatgaat
     9061 tgatgatacc atatcccctc atcgactgct agataagaca atgatgcaag gcatcatgtc
     9121 cataggtcct ggtcagttct gcgctcctct tggcagcgac aagaagatat tatggaccat
     9181 acctaggaac ctgctgcagc caacctttga ctttaccata gcgaataagg tcatctattt
     9241 tttcaacctc ctgccacaac agcttcatca tatagtttct cagccggtgg cttgatgata
     9301 actttttgaa ggccctcaca tgtaatgtgc ctgcaccctg catgcccatc agtgtcatac
     9361 gataaatgtg cgagtcttcc cacaggtctt tattatctaa agctgcagtg atttcaccag
     9421 gacgtttagg caaggtgata ccaaagtaaa ccagttcctt ttctaattgt ttaatttggt
     9481 ctttcagaag ttgaatccca gctgtaaata tcactttcag gtcgacatct ttgacaatat
     9541 tggtcataat ttccgtagag tgcagcgtgt catagcgata ggttagcata tcccaaagac
     9601 acccggcttc gccacagtga attttttccg ttgtatcggc aggaagatgt tgataggctg
     9661 gcgggatacc gacccagcca cgtatagctg cctgtttcat taacatatcc gtacttttta
     9721 aagtgcgcaa aagcatttct atgatcactt tgcgtaaatt atcgtttgtg ataatactat
     9781 aaacagctaa aactaggttt tccacatgtt cctgttcata cagtaacatt tgcatcgcaa
     9841 ttgcttcatc agtcattaaa tcttgattgg tataatttac gacagctgct ctatttttgt
     9901 ttggcgaaat aatattatat tttttcattt gcttttgcaa aatagtgata ttttcttcaa
     9961 tctccagctc tatttttttc agcaacaatt ttaattcagc gtcttctgtg aaagaatcac
    10021 acattaatag ttgttctaaa gtcgtgtact ttgagttcag tatgtcccac aaggtaaaag
    10081 cctcacgaat atcgacatca ttagccttct tttctggctt tttagtcagc agtgcagtta
    10141 ttttcatcat caccctcctc atcaggtcca agtttagcat gtgcaagact tttcttgtaa
    10201 atacgtaacg cgctcaacta aaatgaaggg cagtttgtta tttaaaaaag atcccagaaa
    10261 tcaaaaaatc tgagccgtat ttggcattgg caacgagacc attttcccat tgccccaagc
    10321 ctgcggccac ggccaaaggt cgcagatcac cataaattga aaaaacacct ttggcccata
    10381 tgcggcggta accgtacttt ttaagcagtt tttttatttg ccgcaaattt tgtttagcta
    10441 gccgattaac tctaaagacg tctcgggtca ccttgaagag atctttataa aataaggtgt
    10501 ttttacagta atgaccaaaa agaaaaccgt ggacgccacc acttaactga acataaccac
    10561 gaagaataac ctgatgttca gtactaattg cagaaactgc agccagcatt ttacgattca
    10621 ctatgcagac aactccctta caaaatgtgt tatcacttac acatttgtag tcttttcttg
    10681 aaaggacggg agttatacgc ataaaccaaa aagcgccatg aaatcacttc atagcgcttt
    10741 aaattagttt tgctggttag tcacctagat agccttgcag cgattgatac tcatcacgtg
    10801 tttttcgggg atcgcgaaaa ttaataatca tgttttgttc ttcagtctgg ataatcagat
    10861 aaggc
//
LOCUS       DASRMS010000216         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_732179, whole genome shotgun sequence.
ACCESSION   DASRMS010000216 DASRMS010000000
VERSION     DASRMS010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_732179"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(534..1625)
                     /locus_tag="VFC74_10345"
     CDS             complement(534..1625)
                     /locus_tag="VFC74_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25766.1"
                     /translation="MWANLVNFYRASKSWMPKAFESKSAKQTDYNRFMSANLPNARIA
                     LTKAMASVSACVADAFIVSQGSLRSIQVSKLASSWQTDINLGGLQISPETTIAQFSKA
                     LIDNNNWILAGDQLSFVSYQQYVDANNTPHIICTAYEVTLNIASTEILRDYLPEFCSN
                     SSTAGNLGTNTNISIGGFAYILSRNVGGGALQVSTQQLITNNEVLITQYSTASNVQAA
                     IDSYGVDSDTFLDTGSTSVSRAEQPLFLSYITNGTQRASAGEVFSPSFSDLATTGLTI
                     ALSTEFDTEVSKIKVSQSTGNDSSPYPDITIDGGITHTPGTALINITKERFGDTYFYP
                     SAYVKRIEITLSDGSVITAAFKIGSQSVD"
BASE COUNT          627 a          467 c          525 g          653 t
ORIGIN      
        1 gtcgccatac tcagggcacg tctaatggtt attggcaagg tttccaacgg ctcaagtgac
       61 ccgtttactt ggcaatctct aattgccagt attattgcgc gaaattcctc cgcgctgaac
      121 tctttgtcgg cagacaaaaa atcgtcaaac cacgatgcgt aatacctaat tacgcggtca
      181 tactttctgg gcatacattt tgaaattaag tgaaacaatt tttagacgct gtaaaggtat
      241 aaaaaattcc gctctattcg tcacgaaccg agcggaaaag tgtctaaaaa tgttcaaaat
      301 gtataaaatc gcaccaaaaa cggggtagca atatgctatt atatctttat gttattatat
      361 atatatatta ttattagcta cccctcgggg taattaaagt gcccgttggg aacttatacc
      421 ccccccccgg ggtagtttaa ctaccccatc aaaaaatggg gtgttcctac ttttgacaca
      481 cccaaggcag aagaacctgg ggaagcgccc ggggggaggt acgaaaatta tgtttagtct
      541 accgactgag aaccaatctt gaaggctgcc gtaataacag aaccgtcgga aagggtaatt
      601 tcaatgcgtt taacataagc gcttgggtag aaatatgtat ctccaaatct ttctttagta
      661 atgttaatta gtgcagtgcc aggagtgtga gtaatacctc catcaatagt aatgtctggg
      721 taagggctgc tatcattacc cgttgactga ctaactttaa tttttgaaac ctctgtgtca
      781 aactctgttg acaaggcaat agtcaaacca gtcgttgcaa ggtcactaaa tgacggcgag
      841 aaaacctctc cagcgctggc gcgttgtgta ccgttagtta tataactaag gaatagtggc
      901 tgttctgcgc gtgaaactga tgtagagcca gtgtcaagga atgtatcaga atcaactccg
      961 taactatcaa tggcagcctg cacattgctt gctgttgaat actgagttat cagcacctca
     1021 ttgttggtta taagttgctg agttgacact tggagagcac cgccaccaac attacgtgac
     1081 agaatatacg cgaaaccacc tatactaatg ttagtgtttg taccgagatt gccagccgtt
     1141 gaagaatttg agcagaattc ggggagatag tctcgtaaaa tttcagttga cgcaatgttt
     1201 agcgttacct cgtaggcagt acaaataata tgtggtgtgt tatttgcatc tacatactgt
     1261 tgatagctca cgaatgataa ctggtcacca gcaagtatcc agttattgtt gtcaataaga
     1321 gcttttgaga actgcgcaat tgtagtttct ggactaatct gcaatccacc caggttaata
     1381 tcagtctgcc aagaacttgc cagcttagac acctggatac ttcttaaaga accttgcgaa
     1441 actatgaatg cgtcagcaac acacgcgctt accgaagcca tagcctttgt aagtgcaata
     1501 cgtgcgtttg gaaggtttgc actcatgaat ctgttataat ctgtctgctt ggctgatttg
     1561 ctctcgaaag cttttggcat ccagcttttg gaagctctgt agaagtttac aaggttagcc
     1621 cacataactc tgcgtccttg ctgtgatgga gaacgtctgg cactctcacc ataatttgag
     1681 ctattgagag cctggcgagc tatctgcctg tttaaactta catagtatgt agtgtcacca
     1741 atgcgacctt taatctttcg atttgctgag ttacgaataa ttcccataat ccatttatat
     1801 taagttagac ataaagaagt gttgcaacgg cacaaagata cacattatat attatatacg
     1861 cacgcgcgcg aaggtaaatt taacactttg tacgttgaaa ttgtaatatg aaggtttaaa
     1921 tttgactata ttatgtgttg aatagcaaac ggatgtgtaa gtttatgttt agtattagat
     1981 tagttaagat gaacggacgg cagcacatca gagcttcgct cgcttcgctc gctcgcgttt
     2041 acgtggggtg gtgtctctgc tggggagtgt gtctgcgaag tgcagagaca cccctcaaaa
     2101 agggggagct gctgaagcta agggaggtgc cgccgcttga tttggacgcg ccccccccaa
     2161 acaccctttg ccattgtggg tctgtgctga tgtcctttat gtggtgtgca gatgcgcccc
     2221 taaaaagact ctttggcggg ggggctgggg ggtggagtgg tgtgcgcgtt gg
//
LOCUS       DASRMS010000217         1671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_800718, whole genome shotgun sequence.
ACCESSION   DASRMS010000217 DASRMS010000000
VERSION     DASRMS010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1671
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_800718"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..767
                     /locus_tag="VFC74_10350"
     CDS             <1..767
                     /locus_tag="VFC74_10350"
                     /inference="COORDINATES: protein motif:HMM:NF024296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FctA domain-containing protein"
                     /protein_id="HZK25767.1"
                     /translation="IDGVYTVSVTGAGSTSFPAISYDRVGSYTYTLYQEPGENDKAVY
                     DDTVYLLTVYVTNQENGTGLDTQWVLRLPQTEDKQEAEFTNFYEAVLPVTPPLIQLEK
                     LAKESSFANAGDLIHYYFIVTNDGMVPVVKLIVNDPKLGIDNLIIDLKQDPLMPGETY
                     TYEFSQAYVVTQADVEAGKVDNTLTVKGENEEELAYEATDDLTITREQILPHIPKTGE
                     SRTYWTGIAALMLGGAVALILINRRRNLKIQETDQE"
     gene            complement(1222..>1671)
                     /locus_tag="VFC74_10355"
     CDS             complement(1222..>1671)
                     /locus_tag="VFC74_10355"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HZK25768.1"
                     /translation="TTRAPAPTTQAPVVTTAPTTTKAPAATVDGSFNRSSCDEFIALL
                     NQYRAGKGVGALQKSSSLTGVAEKRSVEIVSNFSHAGIAKYGNYGENIFMGAGAERYN
                     YASTALDAFKKSAGHDTNQLYAAYNYVGVGHYITPSGAHYWTVIFSF"
BASE COUNT          490 a          357 c          411 g          413 t
ORIGIN      
        1 gtatagatgg cgtttacacc gtatccgtca ctggtgccgg cagcaccagt ttccctgcca
       61 tcagctatga ccgggtcggt agctacactt ataccttgta tcaggagccg ggtgaaaatg
      121 ataaggccgt ctatgacgat accgtgtatt tgctgaccgt ctatgtgacc aaccaggaaa
      181 atggcaccgg gctggataca caatgggtgc tgagactgcc ccaaacagaa gataagcaag
      241 aggctgagtt tacaaacttt tatgaagctg tactgcccgt tacacctcct ctgattcagc
      301 tggagaagct ggccaaagaa agcagcttcg caaatgccgg agaccttata cattactact
      361 tcatcgtaac caacgacggt atggtgccgg tagtgaaatt gatagtcaac gatccgaaac
      421 tgggtatcga caacctgatt atcgacttga agcaagatcc gctgatgccg ggagaaactt
      481 atacctatga gtttagtcag gcttacgtgg tcacccaagc agatgtcgag gcaggaaaag
      541 tagacaatac cctgacggtg aaaggcgaaa atgaggaaga actggcgtat gaggcaacag
      601 acgatctgac gatcacaaga gagcagattc tgccgcatat cccgaaaacc ggtgaaagca
      661 gaacatactg gaccggcata gcagcactga tgttgggcgg agcagtagca ctcatactga
      721 tcaacagaag acgtaacctg aaaatacagg aaactgacca ggaataaaat agctaattga
      781 tgcgtgcata gtttcgtgcc tggcaccaaa ttatgcacca tgagttcaga aggggcgttt
      841 cggaagaagc gccctttctt attttgtttt tggacctgct ttttggcttt aaaataagtt
      901 ataataagta aaagtcgaaa tctacaaaaa ctcttattgg atttgaggtg aatcacatgg
      961 acgagaaaaa aacctgtgca gatcaggcag ctgagaaagc cgaagtgatt aaaaaggagc
     1021 ctaaagaaca ggagcattct tcttctgagc acgaaagatg cgcggactgt tctaatccaa
     1081 attgtcaatg ctagttagat aagcagaaga cagagaactt tattacactt gtttttttaa
     1141 aaattaagat ggcataaggg ggcaaaataa atatgccccc tttttaatgc ctgacccctg
     1201 gcagtttgta gattttttta tttaaaagct gaagatcaca gtccagtaat gtgcgccgct
     1261 gggggtaatg taatgtccga caccaacgta attataagcg gcgtaaagct gatttgtgtc
     1321 gtgccctgct gattttttga aagcatccag ggcggtggaa gcataattat atctctccgc
     1381 tcctgctccc atgaaaatat tttcaccgta attgccatat tttgcgatac cggcatgact
     1441 gaaatttgaa actatctcta ccgagcgctt ttcggcgaca ccggttaagg aactgctttt
     1501 ttgcagagca ccaacgcctt tgccagcacg atattgattc agcaaggcga tgaattcatc
     1561 acaactagag cggttaaaag agccgtcaac agtagctgcg ggtgccttgg tcgtagtcgg
     1621 agctgttgtc actaccggtg cctgagtggt cggtgccgga gctctggtgg t
//
LOCUS       DASRMS010000218         1787 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_399237, whole genome shotgun sequence.
ACCESSION   DASRMS010000218 DASRMS010000000
VERSION     DASRMS010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1787)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1787)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1787
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_399237"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1473
                     /gene="rsxC"
                     /locus_tag="VFC74_10360"
     CDS             139..1473
                     /gene="rsxC"
                     /locus_tag="VFC74_10360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003419040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HZK25769.1"
                     /translation="MSLKTFKGGVHPGHHKGATEKKPVVPAKAPSVAIIPLSQHIGAP
                     CEPLVAVGDVVKKGQKIGDSKGFISAPVHASVSGKVSKIAPCNTALGRTVDAVFIAND
                     FQDTWHEEVKPNESWEQLTVDELKKVVREAGIVGMGGATFPTHVKLSPPAEQKIEYVL
                     VNAAECEPYLTADHRAMLERAEDLIVGLKLVMKAVNAPKGYIGIEDNKPDAVAVMQKA
                     AAGEPNIEVYALHTKYPQGAEKQLIKVLTGREVPSGGLPAAVGCVVQNVGTCIAIADA
                     VRFGVPSIERIVTITGSGIKEPKNMLVRVGTPLEQVVEECGGFTDDLRKVILGGPMMG
                     FAQAGTENLPVTKGTSGILCLTDKEVEVDELHPCIKCAKCVEVCPVSIMPIFIGSSVE
                     QGMYDLAETYNAMDCIECGCCSYICPAKRPLTQWIRMAKGEIAAKRRAAAAK"
     gene            1494..>1787
                     /locus_tag="VFC74_10365"
     CDS             1494..>1787
                     /locus_tag="VFC74_10365"
                     /inference="COORDINATES: protein motif:HMM:NF015100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HZK25770.1"
                     /translation="MDKQLVVSSSPHLKSAETIKSVMVDVLVALVPAAIAAVVFFGTR
                     ALLTMVLTTLSAMLTEALILRNKNIFGDGSAAVTGLLLAMTLPPAPPWWLCVLG"
BASE COUNT          476 a          359 c          471 g          481 t
ORIGIN      
        1 aattatacat aaaatagtag atatggcata attaaatttt atggcaaggg caggattatc
       61 tctgaccttc tcgaatatgt ctatggttag gcctttattc ttcaatactt taatcattta
      121 aaaggggtga attgacaaat gagcttaaag acgtttaagg gaggcgttca tcccgggcac
      181 cataaaggtg caacggaaaa gaagcctgtt gttcctgcca aagccccgtc tgtggctatt
      241 attccactct cacagcatat tggtgcacca tgtgagccac tggtggctgt aggtgatgtg
      301 gtaaagaaag ggcaaaaaat cggtgatagt aaaggattta tttctgcacc ggtacacgct
      361 agcgtttcag gcaaagtaag taagatcgct ccctgtaata ctgccttagg gcgaactgtc
      421 gatgctgtgt ttattgccaa tgactttcaa gatacctggc atgaagaggt taagcccaac
      481 gaaagctggg aacagctgac agttgatgaa ttaaaaaagg tggttcgtga agcaggtatt
      541 gtgggcatgg ggggggcaac atttcctacc catgttaaat tgtcaccgcc cgcagaacaa
      601 aagattgaat atgtactggt taatgcggcg gaatgtgagc cctatttgac agcagaccat
      661 cgtgccatgc tggaacgggc agaagatcta attgtcggcc tcaaattagt aatgaaggct
      721 gtgaatgcac cgaaaggata tattgggatt gaagataata agcctgatgc cgtcgcagta
      781 atgcaaaagg ccgctgccgg tgaacccaat attgaggttt atgctctgca taccaagtat
      841 ccgcaagggg cagaaaaaca attaattaag gtattaactg ggcgtgaagt gccctctggc
      901 ggcttgcccg cagctgttgg ctgtgtagta caaaacgttg gtacctgtat tgctattgct
      961 gacgctgtgc gtttcggtgt gccttcgatt gagagaatcg tcacaatcac cggttccggc
     1021 attaaggagc ccaagaatat gctggtgcgc gtaggtactc cgttagaaca agtagtggaa
     1081 gagtgcggtg gttttactga tgatctccgt aaagtaattc ttggcggccc aatgatgggg
     1141 tttgcccaag caggcacaga aaatttacct gtaaccaagg ggacctctgg gatcctttgt
     1201 ctgacagata aagaagttga ggtcgatgaa cttcatcctt gtattaagtg cgccaaatgt
     1261 gtagaagttt gtcccgtaag tattatgccg attttcattg gctctagtgt agagcagggg
     1321 atgtatgatt tggctgagac ctataatgcg atggactgca ttgaatgtgg atgctgttct
     1381 tacatttgcc cagcaaagcg gccgcttaca cagtggattc gcatggctaa aggtgaaatt
     1441 gccgccaaaa gacgagccgc tgcggccaag taaggtaaag gagggatacc ctaatggata
     1501 aacaattagt tgtttcatca tcgccgcact taaaatcagc ggaaactatc aaaagtgtga
     1561 tggtggatgt tttagtggca ttagtaccag ctgcgattgc cgctgtggtc ttttttggta
     1621 ctcgtgcgct gctcaccatg gtgcttacca ccctgtctgc catgttgaca gaagcactta
     1681 ttctgcgcaa caaaaatatt tttggcgatg gcagtgcggc tgtcaccggt ctgctgctag
     1741 ccatgacttt accgccggcc cccccttggt ggctatgtgt tttaggt
//
LOCUS       DASRMS010000219        23960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_128067, whole genome shotgun sequence.
ACCESSION   DASRMS010000219 DASRMS010000000
VERSION     DASRMS010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 23960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23960
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128067"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            56..655
                     /locus_tag="VFC74_10370"
     CDS             56..655
                     /locus_tag="VFC74_10370"
                     /inference="COORDINATES: protein motif:HMM:NF016196.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 family protein"
                     /protein_id="HZK25771.1"
                     /translation="MIFIFLPLTGAVIGWITNVLAIRLLFRPQKPWRLWPLPFTLQGL
                     IPRRRTELAVNIGEIVARELFSLDELPAHLDLSQLQEEVKQFVREAVERWCEQKMGFL
                     PQSVRNYCSQMLRERLADEVARQFPVLADSMFTHMQQQVDVRAVVIEKVNALALTEIE
                     TLVLQVAHRELKQIEWLGAVLGFIIGIFQALLTLFIGKA"
     gene            726..2633
                     /gene="thrS"
                     /locus_tag="VFC74_10375"
     CDS             726..2633
                     /gene="thrS"
                     /locus_tag="VFC74_10375"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003519104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HZK25772.1"
                     /translation="MLKVTLRDSSVREVPTETRITDILGELGGRLKKEALAAVVNGVV
                     VDLQHLLTEDADLRFVTFADEAGQDVYRHSSSHILAAAVKRLYDNVKLGIGPSIKDGF
                     YYDFDYQNSFVPEDLEKIEAEMAKIIKEDLPFERFELPREAAKAFFAELGEDYKVELI
                     RDLPIDAVISFYRCGDFVDLCAGPHLTSTGKIKAVKLLSLAGAYWRGSEKNPMLQRIY
                     GTSFPKKSLLEEYLVRIEEAKKRDHRKLGKELDLFSIQDEGPGFPFLHPNGMIIRNEL
                     EAYWREEHQKAGYQEIKTPIILNRVLWEQSGHWDHYKDNMYFTKIDDLDYAVKPMNCP
                     GAMLIYKTKMHSYRDLPIRMGELGLVHRHELSGTLHGMMRVRNFTQDDAHIFMLPSQI
                     KDEVKKVIALTEKIYATFGFDYHLELSTRPENSMGSDEMWEVATNILREVLEERGLPF
                     VINEGDGAFYGPKIDFHLTDSIGRTWQCGTVQLDFQMPSKFELTYVGEDGQKHTPSAL
                     HRVIFGSLERFMAVLIEHFAGAFPVWLAPRQVVVLPISEKHHAYALSVVESLQQAGVR
                     VEADLRNEKVGYKIREAQVQKVPYMLVVGDKEMAAQEVAVRERSAGDVGSLALEAFIS
                     KVQEEIAAKAH"
     misc_feature    2662..2781
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2869..3369
                     /gene="infC"
                     /locus_tag="VFC74_10380"
     CDS             2869..3369
                     /gene="infC"
                     /locus_tag="VFC74_10380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HZK25773.1"
                     /translation="MLVNEQIRAKEVRVIDSEGNQLGVMSAREAVQKAMERNLDLVAV
                     SLNAKPPVCRIMDVGKYKFEQSKRDKESRKNQKIITVKEVKVRPKIEEHDFQVKLKNA
                     RRFLTAGDKVKATVMFRGREISHPELGRKLLERLAEEVADLSSVERFPKVEGRNMVMI
                     LAPKSN"
     gene            3387..3581
                     /gene="rpmI"
                     /locus_tag="VFC74_10385"
     CDS             3387..3581
                     /gene="rpmI"
                     /locus_tag="VFC74_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HZK25774.1"
                     /translation="MPKMKTHRGAAKRFSKTGSGKFKRSRANHSHILEKKNPKRKRRL
                     NKSTLVSVSENKRVARMLPY"
     gene            3617..3970
                     /gene="rplT"
                     /locus_tag="VFC74_10390"
     CDS             3617..3970
                     /gene="rplT"
                     /locus_tag="VFC74_10390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HZK25775.1"
                     /translation="MPRVKRGVATKARHKKVIKLAKGYFGAKSKLFRTANQAVMKSLS
                     NAYRDRRLRKRDYRKLWIARINAAARMNGMSYSRLINGLKRAGVDINRKIMAELAVND
                     AQGFAELVSVAKNNL"
     gene            4036..5382
                     /locus_tag="VFC74_10395"
     CDS             4036..5382
                     /locus_tag="VFC74_10395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015379 - potassium:chloride symporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HZK25776.1"
                     /translation="MHINPFQASKVSPAKILVGGFALLIVLGTLLLSLPQAVPTGRLP
                     LGDALFTATSAVCVTGLVVVDTGTQFSRFGQTVIMLLIQFGGLGIMTVATIIFTLLGR
                     KISLRERLVIQEALNQETLHGLVRLVKTVVLLTFAAEALGALLLATRFIPIYGWRTGV
                     FYSVFHAVSAFCNAGFDIIGSYRSLTPFATDPVIVLTIALLFIIGGIGFTVWIEFLQR
                     RSIARLSLHAKIVILMTVILLLGGTLIILAVEATNPQTFGQFDWGGKLLNAFFMAATP
                     RTAGLNTVPTNLLLSPTIFLLIVLMFIGASPASTGGGIKTTTFGVLMVTVYSAIRGDE
                     DVLWQQKRIPGDLIRRALTITVVAMLLVCLVTFLLLVTEDGSFTFLDYLFEATSAFAT
                     VGLTTGVTASLSSWGRLFIIVTMFAGRVGPLTLVFALARHTRKTSVRYLPERIMIG"
     gene            5403..6059
                     /locus_tag="VFC74_10400"
     CDS             5403..6059
                     /locus_tag="VFC74_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011100421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HZK25777.1"
                     /translation="MKAKQFAVIGMGRFGSSVASTLYEMGHDVLAIDSEEEKVENIVE
                     KVTHAVVADTTSEAALKSLGITNFDVVIVAIGEDIQASILTTLVLKELGVKHIVAKAK
                     AMQHGKVLQKIGADRIVFPERDMGIRVAHNLVANNVLDFIELSPDYSIMEIIVPDEMV
                     GKSLLELDLRGKYGANVMAIRSEDRKINVSPIATDRLHAGDILIVLGNNDNMQKLGIS
                     "
     gene            6060..6851
                     /locus_tag="VFC74_10405"
     CDS             6060..6851
                     /locus_tag="VFC74_10405"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HZK25778.1"
                     /translation="MVLIESLHNQQLKLYRSLAKRKYRQKYGLLPLEGTRLVGEALLQ
                     KVAQPKFILLRGEATLEDFALPCAPPVTTTVLNVAEKVFAQTAFTESPQGILAVVEQP
                     QMPLSAVFTQQPALILVVDGVQDPGNLGTMIRSAAAVGASGVVLLPGTVDATNPKTLR
                     ASMGAYFALPVVETSFTTLHSSLQKHQVQLVITGSQAARSYEDYDWQQSVAVVIGNEG
                     AGVSPPVREAAATAVAIPMAQAVESLNAAIAMSVLFFEAARQRRQ"
     gene            6979..7077
                     /locus_tag="VFC74_10410"
     CDS             6979..7077
                     /locus_tag="VFC74_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqzL family protein"
                     /protein_id="HZK25779.1"
                     /translation="MISAEFFWIVFERTGSVAAYLLYRQLITARLC"
     misc_binding    7082..7308
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7478..8503
                     /gene="pheS"
                     /locus_tag="VFC74_10415"
     CDS             7478..8503
                     /gene="pheS"
                     /locus_tag="VFC74_10415"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012033106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HZK25780.1"
                     /translation="MQEKLQAIATQARAEITAANTQEILKQVQVRYLGKKGEMTSLLR
                     GMGQLSPEERPLIGSLANKIRDELNELFATRQNELRQEERLAQMAKQRLDVTLPGRPQ
                     ALGYLHPLTQVMDEIGEIFLGMGFTIDEGPEVETDYYNFEALNLPKDHPAREMQDSFY
                     FSPEMLLRTHTSPVQVHTMERVAPQVPVRIIAPGKVYRRDDDATHSPMFHQVEGLVID
                     RNISLADLKGTLLLFAQQMFGPHQQVRLRPSFFPFTEPSAEVDISCMVCAGKGCRVCK
                     DTGWIEILGSGMVHPRVLAMSGYDPEEYTGFAFGMGVERIAMLKYGVDDLRLFFLNDL
                     RLLRQFR"
     gene            8527..10941
                     /gene="pheT"
                     /locus_tag="VFC74_10420"
     CDS             8527..10941
                     /gene="pheT"
                     /locus_tag="VFC74_10420"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HZK25781.1"
                     /translation="MRVPYTWLKDFIEIELTAEELAETLTRAGIEVDEIVTLAPRFSE
                     VVVAEVISIDKHPQADKLFVTQVSDGQDTMTVVAGIDNIKAGDKVPLAKPGAKLPGDV
                     KIKRTKLRGIESHGMLCSAEELALSLNPGINGILVLDPETPVGQPLADALGLNDPILI
                     LDLTPNRADCLGLVGVAKEVAALTGKRVQLPDNTLTINLAQAEQIVPKIRIESPELCA
                     RYTGLVIKDIKIALSPIWLQIRLLQAGVRPISNIVDVTNYVMWEWGQPLHAFDYQTLA
                     AQTIVVRRAETEEKLVTLDNNERSLSNEMLVIADAEQAVAIAGVMGGLATEITDETQA
                     VLLESAHFNPVSIRRTGRSLGMYSEAQQRFEKGVDVNGCVNAMQRAARLIELLGAGSV
                     AGEIVDEYAAPSYPRQVRLRPERARKLLGLEISQREMTAIFQRQGFTVEEGTYLHVMV
                     PTLRADLQEEVDLIEEVARIYGYDKIDTSLPGGQLIQGRLTEKQRALAKTRQLLVACG
                     LNEAICYSFVSPQQFDKLNLTAADPLRKTLTIANPLSDEQSVMRTMLTGGLLDALVYN
                     QNRNQHNVKLFELGTVFLAADVPLAQQPEEKTMLGLALMGSFPAEHWQEKPQAVDFYT
                     LKGIVENLFARLGVTGYSFKASELSWCQPGQTAEVWLDDVKLGWLGHLHPEALDNYDL
                     AQPVFVAELDMHYILARVQLVAAYSSLPRYPAVLRDLAVVVPDDTTAAEVVRVIREAG
                     GKLVEDVALFDQYRGEQIPQGHRSLAFAVTYRDADRTLNDQEVTKVHQQIEAALGQLL
                     GAGLRG"
     gene            11027..11293
                     /gene="zapA"
                     /locus_tag="VFC74_10425"
     CDS             11027..11293
                     /gene="zapA"
                     /locus_tag="VFC74_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HZK25782.1"
                     /translation="MSQLPEKNRVSVKIYEEEYTMRSSASPEYMRRLASYVDEKMKQI
                     GQTNSRLGINKVAVLTSVNLADELFRLRKEVRELENLLKKKNSK"
     gene            11290..11838
                     /locus_tag="VFC74_10430"
     CDS             11290..11838
                     /locus_tag="VFC74_10430"
                     /inference="COORDINATES: protein motif:HMM:NF014704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HZK25783.1"
                     /translation="MMNWFDFLLVAIVVFFAIVGWQKGLVRQLFDLLGVIASYFVALR
                     YSNQFVYWLNQYLPLTLWFNQLFPTPTAWGIDLGAVLIRLVGFALLFGAVSLVFRTTG
                     AAVHSIFSLPILDLVNGLGGLLLGAIRGVFLALILIGAANLIGTPFLARAVTESAIAT
                     TVLAVLPVVYEQMLRLLFTDFV"
     gene            12050..13585
                     /locus_tag="VFC74_10435"
     CDS             12050..13585
                     /locus_tag="VFC74_10435"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HZK25784.1"
                     /translation="MRNRDVAEMLDRAAQILALRGENRYRIRAYKKAARAVASLDERI
                     DVLVLERRLQDLEGVGPALADKIKEIVETGKLAMLERLETAKLPLAVTNRSILLASAL
                     TFSAELLPALVALPGVKQTQVVGQTRRRQETVSGLEFVLTVSDMVQAKKALSAYPYLH
                     QQQWQEDRCLAVHAYGMEITLYLTAEEKFVQQLWQTTGSAQHVQKIAALVKEKSGYDL
                     LTAADKEKKCFATEAEIYALAGLPEIPPELREDSGEVEAAQLGQLPHLIEAADYRGDL
                     HLHTNWSDGTASVEKMVETAVELGYEYLAITDHSQSLKIARGLSLERLKEQKAFIRSL
                     QNKYNIRIFAGIEADILDDGSVDASDEVLAELDVVVASVHSGFKQSREKITRRICRAL
                     QNPYVHILGHATGRLLGKRDPYDVDLAEVLRTAAATGTSLEINSSPDRLDINDQVARQ
                     ANEAGINVAINTDAHSQLELANVILGLAMARRGWLEKSDVLNTHSAQELLHLLRHKSK
                     QRG"
     gene            complement(13582..14175)
                     /locus_tag="VFC74_10440"
     CDS             complement(13582..14175)
                     /locus_tag="VFC74_10440"
                     /inference="COORDINATES: protein motif:HMM:NF016154.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YetF domain-containing protein"
                     /protein_id="HZK25785.1"
                     /translation="MTELLTQSIKLAGRSLAIYLVTLIMVKLMGKREVGQLSPLDFVV
                     GVIIGSVAAAPMVDLQLPLLPTLIPLVTLGLLEILAAVLALNNQKIRLFLEDNPTIII
                     ADGQIIMANMAKVRMNIDDLKQELRKLGIADINEVEEGVLESGGTFSVIKKKEHQPLT
                     PQDLQTVTLHNLDRVVRSHLHKNRADLKIMVEQLGQR"
     gene            complement(14180..14728)
                     /locus_tag="VFC74_10445"
     CDS             complement(14180..14728)
                     /locus_tag="VFC74_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25786.1"
                     /translation="MGNLQKDQQTFAELDRTRKETLAQADNVTNQQLQLNPQPTPQEE
                     LSQLVSQLESSVRQQQLKTEQTIQQTLQQASTALNDAQNIDALFLQVQTLQQAINQPG
                     GSNNPQSYPQLLQQLESKVRQQLQQADEQLAQALQQTVSSMAQAQVTMTDKQTYNSLV
                     EKLKQCEKVVQKWQTTNPPAVH"
     gene            14854..15444
                     /locus_tag="VFC74_10450"
     CDS             14854..15444
                     /locus_tag="VFC74_10450"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HZK25787.1"
                     /translation="MKRTYTKQLENNFFHRPTEDVARALLGKVLVHQGTAGVTAGRIV
                     EVEAYLYRNDAACHAARKQTIRNRPMFGPPGTAYVYLIYGLHYCFNVVTGLEGEGEAV
                     LVRALEPLAGLNIMAERRGTAVTKKMAAGPGNLCRALAITKAHNGHNLNRPPLYLADD
                     DFVVSELVTTTRVGINVATDLPLRFYIANNPFISRK"
     gene            15449..17788
                     /locus_tag="VFC74_10455"
     CDS             15449..17788
                     /locus_tag="VFC74_10455"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HZK25788.1"
                     /translation="MKREIRLLEFNKIRQLLAEQCLTPLGRELAEDLVPALDVNLCRR
                     WQNETTEAASLMTRHQLGLENVPDLRSVFSLAGRGSLLGEEQLGAVLRLLLAATRVKL
                     FFKEKEEYPVLAGLAAAMVMLPELREELKQTIDEEGRLRDNASPELARLNRALRNGEH
                     DLRDRFDRLIKSPANQKLLQESLITMRGDRLVVPVRVEYRAQVLGVVHDQSASGATLF
                     VEPLWAVEANNKLAVLRREAEREKERILARFSQWVGGEAEGLTTTLTLYAELDFIMAK
                     GRQSLKLKGVQPQLNNQGYLKIIQGRHPLLIGDVVPISLEMGEHLRTLVITGPNTGGK
                     TVTLKTVGLFSLMAQSGLHVPALEGTELAVFPKICADIGDEQDISQSLSTFSGHLKNI
                     VEILQTLVPGALVLLDEVGAGTDPTEGAGLAMAILAYLHQHGAVTVATTHYSQLKSFA
                     YLTPGMENASVEFDVATLRPTYQLLVGVPGVSNAFNIAKRLGLPDEIIDQGREFLSQE
                     ETHLEEVVADLVADRKRLEYVSLQAEDERQQALSLLTKVEREQEELKLRKDEILAKAR
                     AEALEIVVRAKRETQQLLKELRRLAAQSLPPAADQIEESERKLQQLNEEVRDFTAPAP
                     TGQPLTLQELAPDMEVLVTSLGQRGIIVQVTEQQIQVQIGSMRVSVDPSELTPIAAAA
                     AKAKPEIKVTSLYVRRDVPPEIDLRGHTFAEAALEVDNYLDEAAMAEHKEVRLIHGKG
                     TGRLRAGLKDYLQGHPHVASLRMGHPNEGGTGVTVVEIK"
     gene            complement(17874..20576)
                     /locus_tag="VFC74_10460"
     CDS             complement(17874..20576)
                     /locus_tag="VFC74_10460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HZK25789.1"
                     /translation="MSKETKEGKNNHNKNGANKNLRKQRLKRLFLILSIIFVVFAAVT
                     AITTYAAVKYYMADLPPFDPTELEPAQTSFMYDKDGELVTPLMGAENRIIVPLDQMSP
                     DLVDAFIAIEDDRFYDHPGFDARGMFRAFIYNLTNQSGKMQGGSTITQQLVKNTFLTP
                     ERTIKRKLQELYLSYQLERIYSKEEILEFYLNRIFFDFNAHGVEAAAQTYFGKSANDV
                     TLGEAAMLAGIPNLPGKYSPYRDFEEAKKRQEIILTRMAEINAITQAEAEAAKAEEIV
                     LSGIPERSYPHPWFVDHVILDEAPEKLATLPEFADLTIAEIKTKLYTSGLHIYTTLDQ
                     RIQQDVVNILDNPKYYRKSFRVEETKPIQPQTAVVVADPTTGHVAALVGGRLYETEIN
                     VFNRSTKGRMQAGSVLKPIIAYGPAFNEGLASPGTVLDDAPKVFKGNYFPNNYDMSFR
                     GLVTVRNAIANSYNIPAIRLLDQLGVEKGKEYAKRMGLTSLTNDHGLSMVVGGLNEGV
                     SVWDTTQAFSVLANEGIRTDLTTITKIVDSDGNVLYEQEPNKEEILSPQAAWLTTSAL
                     IDTVRIGTASALRIGRTVAVKTGTSEYAHDAWMAAYTPQYVTVFWMGQDSWPKPKAEG
                     DYRSFEVTPRFMNPILKAVHKDLKKVEFKRPDGLRKVTICSKSGLRPGEFCPDEHIVT
                     DWFTSSTIPKGTCEMHVELEVCSISGLWPTEFCPQELLETKAFLNRPEYIKTDGRWPG
                     RSGRTPRDAELMPPKEDELCDKHTSRPEDVKNLKAKQVSDNGNVELSWKAVNGASGYL
                     IARKGPGEGAFVSLTSSPVTATTFTDQGLAPGAYSYQLTAISAEGVKSSTTTTTIKVE
                     PPPPPPPPPVEEEPPVEPPPGQEEPPPDDDDDDDGKGNGNGKGN"
     gene            21000..21659
                     /gene="yunB"
                     /locus_tag="VFC74_10465"
     CDS             21000..21659
                     /gene="yunB"
                     /locus_tag="VFC74_10465"
                     /inference="COORDINATES: protein motif:HMM:TIGR02832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sporulation protein YunB"
                     /protein_id="HZK25790.1"
                     /translation="MLRRRLIVKRETRVFFLLALLIFLMIRMFMLIEHNLRPTILRSA
                     GIRANYIATEAVNKAILENVAQDVDYQDLILLVKDNSGRIVFSQVNNVTISGIIAATN
                     LHAQEALKTVAREKISIPFGQVFNSYLLANVGPRIPVKISPRGVINTNLIDKFESAGI
                     NQVRHKIYLEVQAEMQVVIPLMADKINVSTTILLVDAIYPGEVPNTVIDLQYPLSGQH
                     E"
     gene            complement(21662..21967)
                     /locus_tag="VFC74_10470"
     CDS             complement(21662..21967)
                     /locus_tag="VFC74_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25791.1"
                     /translation="MTELATNQDIEFVHEEREDVVEETISNEMAADNETGTVQEEVEK
                     EVTAMSSHQSAAGTPKDNEFKRGFIFGLGFWAAGALVLSVPFGAILFAVYSVMTTML"
     gene            complement(22231..22698)
                     /locus_tag="VFC74_10475"
     CDS             complement(22231..22698)
                     /locus_tag="VFC74_10475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25792.1"
                     /translation="MQRKQLKAVLVMLCSTILLTFYTLFILKSNQASQIFSLGNPLYN
                     VELVHYNNLSADYINLFIICLHITTAAIFYFSKDQLIKGLLYLNCGELVLWVLCLGLE
                     IQLSHTHFKAMASLAQFLSLQLSTNILFLFLMLYSKLKENLLIVRRRKYKYRY"
     gene            complement(22772..23668)
                     /locus_tag="VFC74_10480"
     CDS             complement(22772..23668)
                     /locus_tag="VFC74_10480"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HZK25793.1"
                     /translation="MKTKEKLQAVFAIFVTTVFWGLSYSSTKVLLGTLLPAQIAFYRL
                     LIACAALGLLFLATHKKSVQRKDLLRVMAGGFFGNFIYFLFENNGLRFTTAGMGSLII
                     ATIPVLNVLVGAIIFKEKSNPQRWLGVLLSFIGVYLLIRSGHDGELSFTSLKGNLLVF
                     GAACTWVIYTRLNEPLLQNYDSMVINLYQAATGMFLLSGLALPQSAHAVLLQPEVAFN
                     LLYLGIFCSAVAYFFYLYALQKLGAAAVTSFLNLIPVFGVLGGAVLLKETLVTGQLLG
                     GAIVIVGVSLVTMTGKAQTQQG"
BASE COUNT         6550 a         4903 c         5814 g         6693 t
ORIGIN      
        1 tagtaattgt agtctttgat tttttgttaa aactgtagtt gaaggaagat tgactgtgat
       61 atttattttt ttgccgctaa ctggagcagt tattggttgg attactaacg tattggcgat
      121 tcgtttactg tttcgaccgc agaaaccatg gcgcttgtgg ccgctgccct ttaccttgca
      181 gggcttgatt ccgcgacgcc gtacggagct ggctgtcaat attggtgaga ttgtcgctcg
      241 ggagctattt tcgttggacg agcttcccgc ccacctcgat ctttcgcagc tgcaggagga
      301 agtaaagcag ttcgtgagag aggccgtgga gcggtggtgt gagcagaaaa tgggtttcct
      361 gccccaaagt gtacgcaact attgcagcca gatgctgcga gagcggctgg cagatgaggt
      421 ggctcgccag ttccctgtgc tggcagactc catgttcacg catatgcaac agcaagtgga
      481 tgtgcgtgct gtggtcatcg aaaaagttaa tgcactagct ttaactgaaa ttgaaacact
      541 ggtactgcag gtagcacatc gagaattgaa acagattgag tggctaggag cggtgttagg
      601 gtttataatt ggcatctttc aggcgctttt gaccctattt attggaaaag cttgacacca
      661 cgcaagccag gatttaagat gaaagatata actaattttg aaatgattgt gggatggtga
      721 taaggatgct aaaagtaaca ttacgtgaca gttctgtaag ggaagtgcct acagaaacac
      781 gtatcactga tatacttgga gaactgggcg gtcgcctaaa aaaagaagca ttagctgctg
      841 tcgtcaatgg ggttgtagta gatctgcagc atctcctgac agaagatgca gatttgcgtt
      901 ttgtaacttt tgccgatgaa gcaggtcaag atgtgtacag acattcatca agccatattt
      961 tagcagcggc agtaaaaagg ctctatgata atgtgaagct gggtattggt ccgtcgatta
     1021 aagatggttt ttattatgat tttgattatc aaaattcctt tgtgcctgag gatctagaga
     1081 aaattgaggc tgaaatggcc aaaattatca aagaggactt accctttgaa cgctttgagc
     1141 tgccaaggga agctgcaaaa gctttttttg ccgagctagg tgaagattat aaagtggaac
     1201 tgattcgtga tttgccaatt gatgcggtga ttagttttta ccgttgtggt gattttgtgg
     1261 atctttgcgc cggtcctcat ctgacttcca ccggcaaaat aaaggctgtt aagttattaa
     1321 gtttggctgg tgcctactgg cgcggcagtg aaaagaaccc catgctgcag cgtatttacg
     1381 gcacttcttt ccccaaaaaa tctttgctgg aagaatatct ggtacgaatc gaggaagcga
     1441 aaaaacgtga tcatcgtaaa ttaggtaaag agctggatct gtttagcatt caagatgaag
     1501 gtcctggttt cccctttctt catcctaatg ggatgatcat ccgcaatgaa ttggaagcct
     1561 attggcgtga agagcatcaa aaggctggtt atcaggaaat taagacaccg attattttaa
     1621 accgagtttt atgggaacaa tccggacatt gggatcatta taaggacaat atgtatttta
     1681 ccaaaattga cgacctcgat tatgccgtta aacccatgaa ctgccctggt gcaatgctca
     1741 tttataaaac aaaaatgcac tcttaccgtg acttgcccat tcgcatggga gaactgggac
     1801 tggtgcatcg gcacgagtta tcagggacgt tgcatggcat gatgcgcgtg cgcaacttta
     1861 cgcaggatga tgctcatatt tttatgctgc cctcgcaaat caaagatgag gttaaaaaag
     1921 ttatcgccct aaccgagaag atttatgcca cctttggttt tgattatcat ttagagcttt
     1981 ctacccgacc ggaaaactcc atgggctcag atgagatgtg ggaagtggct acaaatattt
     2041 tacgtgaggt actggaagaa cgcgggctgc cctttgtgat taatgaaggc gatggcgctt
     2101 tctatggtcc gaaaattgat tttcatctta ccgactccat cggtcgtacc tggcagtgtg
     2161 gtacagtgca gttagatttc caaatgccca gtaaattcga gctgacttat gtgggcgaag
     2221 atggtcaaaa gcacacaccg tcggctttgc atagagttat ttttggttct ctggaacgct
     2281 ttatggcggt actaattgag cattttgccg gagcatttcc agtctggctg gcgccgcggc
     2341 aagtagtggt gctgccaatt tctgaaaaac accatgctta tgctttgtcc gtggtagaaa
     2401 gcctgcagca agctggcgta agggtcgagg cagacttgcg taacgaaaag gttggttata
     2461 aaattcggga agctcaagtc caaaaagtgc catatatgtt agtcgtgggt gataaagaga
     2521 tggcagccca agaagttgcc gtacgggaac gtagtgctgg ggatgtaggt tcactagcgc
     2581 tcgaagcttt tatctctaaa gtgcaggagg agattgccgc aaaagcgcat tgacattgtt
     2641 attgctgtat gatatagtaa aaaacgcaag aagaagccac cgcttctcac cccaaggcag
     2701 cgccgcacgg gttacttaaa gtaaaactca aagtttactt tgtgtgaaag caggtggagg
     2761 cacctgcttt tttgttacgt ctaattatga tagtactgaa aagtgtccat agaatgagtg
     2821 cattttcttg taataatctg ctggaggtga tagacaatta gtaaggattt gcttgtaaat
     2881 gaacaaatcc gtgccaagga agtacgggtt atagattctg aggggaacca acttggagtt
     2941 atgtcagcac gtgaggctgt gcaaaaggcg atggagcgga atttggattt agttgcagtt
     3001 tcgctcaatg cgaagccgcc cgtctgccga atcatggatg ttggcaaata caagtttgaa
     3061 cagagcaaac gagataaaga atctcgcaag aatcagaaaa taatcactgt caaggaagtc
     3121 aaagttcgcc ctaaaattga ggagcatgac ttccaggtga agctcaaaaa tgcgcgtcgt
     3181 tttttaactg ccggtgataa agtaaaagct actgtgatgt tccgtgggcg tgagatttca
     3241 cacccggagc tgggaagaaa acttttagaa cgtttggcag aagaagttgc agatttgagc
     3301 agcgtggaac gttttcccaa agtggaagga cgtaatatgg tgatgatttt ggcaccgaaa
     3361 tcgaactagt gtaggagggt atattcatgc ctaaaatgaa aacacaccgg ggtgcagcca
     3421 agcgctttag caagacaggt agtggtaagt ttaaacgcag ccgtgcaaac catagccata
     3481 tcttagaaaa gaaaaatccg aaacggaaac ggcgcttgaa taaaagcacg ttggttagcg
     3541 tttccgaaaa taaacgtgtt gcccgcatgc tcccctatta attgtaaaac ataaacgaag
     3601 caaggaggtt ttactaatgc caagggtaaa acggggcgta gccactaagg cacgccataa
     3661 aaaagtaata aaactggcaa aaggttattt cggggcgaag agcaagctat tccgcacagc
     3721 taaccaagct gtgatgaaat cattgtccaa tgcctataga gaccgtcgcc tgcgtaaacg
     3781 tgattacaga aaattatgga ttgcacgcat taacgctgct gcccggatga atggtatgtc
     3841 ttacagccgt ttaattaatg gcctgaaacg tgcgggcgta gatattaacc gcaaaataat
     3901 ggcggagtta gctgttaatg atgcgcaagg tttcgctgag ttagtcagtg ttgccaagaa
     3961 taacttataa tttttaacca tttaagtcag aagtaaccaa cttctggctt cgtctttttc
     4021 cttggaggtg agtggatgca cattaacccc tttcaagcat ctaaagtatc tccggccaaa
     4081 attttggttg gtggttttgc tttattaata gtgcttggta cactgttatt atctttgcct
     4141 caggctgtcc ctacagggcg cctgccattg ggagacgctt tatttacggc gacatctgcc
     4201 gtttgtgtga ctggtttagt tgtagttgat accggcacac aatttagtcg ttttggacaa
     4261 actgtgatta tgttgctgat ccagtttggt ggtctgggaa ttatgactgt agcaacaatt
     4321 atttttaccc tcctaggtag aaaaatatcg ctgcgggaac gtttggtgat tcaagaagcg
     4381 ctgaatcagg aaaccctgca cggactagtg cgtttagtta aaactgtggt tttgctgacg
     4441 tttgctgcag aagcgcttgg tgccctactt ttagctactc gtttcattcc catttacggt
     4501 tggcggacag gtgtcttcta ctccgtcttt catgctgtct ccgcattttg taacgctggt
     4561 tttgatatta ttggcagtta tcgtagtttg actccttttg ctacagatcc ggtgattgtc
     4621 ttaactattg ctttattatt tattattggc ggcatcggtt ttactgtctg gatcgaattc
     4681 ttgcagcgcc gcagcattgc gcgcttaagt ctacatgcaa aaatagtaat actaatgaca
     4741 gtaattttgc tgctgggcgg cacattaatt attttggcgg tagaagccac aaatccacaa
     4801 acttttggcc agtttgattg ggggggaaaa ctgctaaatg cttttttcat ggcagctaca
     4861 ccacgcacag caggtttgaa taccgtgcca actaacctat tgctgtcacc aacaattttt
     4921 cttttaattg tgttgatgtt tattggcgca tcaccggcat ctacgggtgg gggcattaaa
     4981 acaactactt ttggtgtttt aatggtgact gtatattcag ctatccgtgg cgatgaggat
     5041 gtgctgtggc agcaaaaaag gattccggga gatttgattc gccgtgcgtt aaccattact
     5101 gtggttgcga tgctgcttgt ttgtttagta acatttctcc tgttagtgac tgaagatggt
     5161 agttttacct ttttagatta tctgtttgag gctacatcgg cctttgctac cgtcggactt
     5221 accactggag tcaccgcatc actttcaagc tggggtcgcc tttttattat tgttactatg
     5281 tttgccggcc gcgttgggcc cctaacttta gtctttgcgc tggctcgcca caccagaaag
     5341 acttctgtgc gttacttgcc ggaaagaata atgattggtt aagcaaagaa gaggtggttg
     5401 atttgaaagc aaaacagttt gcagttattg ggatgggccg ctttgggtca agtgtggcta
     5461 gtacgctgta tgaaatgggt catgatgtgt tggccattga cagcgaagaa gagaaggttg
     5521 agaacattgt cgagaaggta acacatgccg tagtggccga tacgaccagt gaagctgcct
     5581 taaaatcgct gggtattact aactttgatg tggtaattgt agctattggt gaagacattc
     5641 aggctagcat tctcacgacc ttagtactga aagaactagg tgttaaacat atagttgcta
     5701 aagctaaggc tatgcagcat ggcaaagtgc tgcaaaagat cggtgcagat cggattgttt
     5761 ttcctgaacg tgatatggga attcgtgtag ctcacaattt agttgccaat aatgtgcttg
     5821 attttattga attatctccg gactacagta ttatggaaat tatcgtgccg gatgaaatgg
     5881 taggcaaatc actgctagag ttggatctac ggggcaaata tggagctaac gtgatggcta
     5941 ttcgtagtga ggataggaaa ataaatgttt caccaatagc taccgaccgt ctccacgcag
     6001 gagatatttt aatcgtctta ggtaataatg ataatatgca aaagttaggg atatcataaa
     6061 tggttttaat tgaaagtctg cacaatcagc agctaaaact gtacagaagt ctggccaaac
     6121 gcaaatatcg gcaaaagtat ggcttgcttc cgttggaagg aacacgcttg gttggtgagg
     6181 cactgctgca aaaggtggcc cagccaaagt ttattttgct gcggggagag gctactttgg
     6241 aggattttgc tctgccttgt gctcctcctg ttacgaccac agttttaaat gtggcggaga
     6301 aggtttttgc gcaaacagct tttactgagt cgccgcaggg tattctcgca gtagtagagc
     6361 aaccgcaaat gccgctgtca gcagtattta cgcagcagcc tgctcttatt ttagttgttg
     6421 acggggtaca agatcctggt aatttaggta cgatgattcg ttcagcggct gctgtgggtg
     6481 ccagtggtgt cgtgctgtta cctggtacag tagatgcaac gaaccccaaa acattgagag
     6541 catccatggg ggcttatttt gcccttcccg tggtagaaac aagctttaca actttacata
     6601 gtagtttaca aaagcatcag gtgcagctag taattactgg cagccaagca gctaggagtt
     6661 atgaagatta tgactggcag cagtctgttg ccgtggtcat tggcaatgaa ggagcaggtg
     6721 tctcaccacc cgtgagagaa gctgccgcta cagccgtggc tattcccatg gcccaagcgg
     6781 tagaatcgct taatgccgcg attgctatgt ctgtcttgtt ttttgaagca gcgagacaac
     6841 gccgtcagta agtcagcaga agcggcctgg gagctcctga aatcagcacg tcctaacttg
     6901 cacctcatac ttgtgtcgga aagttcgata tgctataata agggcaatcc cattccaaac
     6961 ataaggagtg gtccatgaat gattagtgct gagtttttct ggattgtgtt tgaacgaaca
     7021 ggttctgtag ctgcctattt gctttatcgg cagctgataa cagcacgtct atgttaatat
     7081 gctaaaggca atgaagggaa gagtatgcta acttctgtac atacaggaaa gaacgcctta
     7141 gactgagagc gttctgtatg gatatggctg aagttcctcc ccgagccgcc gaattgaagg
     7201 attactgtgt aggttcggta cgggtactcc cgttacagag ttgaagtgag tatgtcgcca
     7261 agtctactaa attgggtggt accgcggaag atttctttcg tcccttaaga ttggggcgat
     7321 ttttattttt tgccagctaa ttagtgcaat actagcaggg tgtgtttact gccaagcgcc
     7381 ataagggagc gattgagcgc gccgttcctg tgtgcgccgc tctactttaa cagttgtcgt
     7441 gatatatact ttatgatatt cgcaaggagt gatgcaaatg caagagaagc ttcaggccat
     7501 tgcaacccag gcacgtgctg aaataactgc ggctaatacg caggagatct taaagcaggt
     7561 gcaggttcgc tatcttggta aaaaaggaga aatgacctca ttattgcggg ggatgggaca
     7621 actttccccg gaggaacgcc ccctgattgg cagtctagcg aataaaatcc gtgatgaatt
     7681 gaatgagtta tttgctacgc gccaaaatga gctgcgtcaa gaagaacgtc tagcgcaaat
     7741 ggccaaacaa cgtcttgatg tgacgctgcc agggcgtccg caagcgctgg gttatttaca
     7801 tcctttaacg caagtgatgg atgaaatcgg tgaaattttc ctcggcatgg gctttactat
     7861 tgatgagggg ccggaagtag aaacagacta ctataatttt gaggccttga atttacctaa
     7921 agaccatccg gcacgagaaa tgcaagactc tttctatttt agcccggaaa tgctcctacg
     7981 gacccatact tctccagtac aagtacatac gatggaaaga gtggcgcccc aggtaccggt
     8041 gcgtatcatc gccccaggga aggtgtatcg tcgtgacgat gatgccaccc attcgccgat
     8101 gtttcatcag gtagaagggt tagtgatcga ccgcaatatt tcactggcag atttaaaggg
     8161 tacgctgtta ctctttgccc aacaaatgtt tgggccgcac cagcaagtac gcctgcgccc
     8221 cagcttcttc cccttcaccg aacctagtgc tgaagttgac atttcctgta tggtttgtgc
     8281 aggcaaaggt tgtagggtct gcaaggatac tggctggatt gaaattcttg gttcggggat
     8341 ggtacatccc cgtgtcttgg cgatgtctgg ctatgacccg gaagaatata ctggttttgc
     8401 ttttggcatg ggcgtggaaa gaattgctat gcttaagtac ggcgttgatg atctgcgctt
     8461 atttttcctt aatgatctac gactgttacg tcaattccgt taacgaaagg cagaggaggc
     8521 attagtatgc gagtacccta tacttggtta aaagatttta ttgagataga gctgactgct
     8581 gaggagttgg cggaaacact gacacgagct gggattgaag tagatgaaat tgtaacttta
     8641 gccccccggt ttagtgaggt agtagtagct gaagtaatct ccattgataa acacccgcag
     8701 gcagataaat tgtttgtcac ccaggttagc gatgggcagg ataccatgac ggtagtggcg
     8761 ggcattgata atattaaggc cggtgacaaa gttcctttgg ccaaaccagg agctaaactg
     8821 cccggagacg taaaaatcaa gcgcaccaaa ttacgcggta tcgagtcgca cggcatgttg
     8881 tgttctgcag aagaattggc gctgtctcta aatccaggca ttaatggcat tttagttttg
     8941 gatccagaga ctccggtggg acaacctctg gcggatgctc tcggcctcaa tgatcctatt
     9001 ttgatacttg atttaacacc taatcgggca gattgcctgg gtttagtggg cgtggctaaa
     9061 gaagtggcag ctttaacagg taaaagagtg caattgcctg acaatacctt aacaattaat
     9121 ctcgctcaag cggaacagat tgtccccaaa atccgcattg aaagtcctga gttatgtgcc
     9181 cgctatacag gtttagtcat caaagatata aaaattgcac tttctcccat ttggctgcaa
     9241 attcgtctgc tgcaagcagg tgtacgcccc attagtaata ttgtggatgt taccaactat
     9301 gtaatgtggg agtgggggca acccctgcac gccttcgatt accagacttt agcagcgcaa
     9361 acgattgtgg ttcgtcgcgc tgaaacagag gaaaagcttg ttactttaga caataatgaa
     9421 cgcagcttgt ccaatgagat gttagttatt gctgatgctg aacaagcagt agcaatagca
     9481 ggagtgatgg gcggtttagc gacggaaatc acagacgaaa cgcaggcagt acttttagaa
     9541 tcagctcatt tcaatcctgt ttccatccgc cggacgggcc gtagtttagg tatgtattca
     9601 gaagcacagc agcgttttga aaaaggtgtc gatgttaatg gctgtgttaa tgcgatgcag
     9661 cgagcagcgc gcttgatcga actgctgggc gccggcagtg ttgccggtga aatagttgat
     9721 gaatacgctg ctcccagtta tccacgtcag gtccgtttgc gccccgagcg ggctcgcaaa
     9781 ttattgggat tagaaatttc acagcgtgaa atgacagcca tttttcagcg tcaagggttt
     9841 acggtagaag aaggaactta tttgcatgtg atggtgccca cgctgcgtgc tgatttgcaa
     9901 gaagaagtgg atttaattga agaagtggcc agaatatacg gctacgacaa aattgacaca
     9961 tcgctgccgg gtggtcaact gattcaagga agactgacgg aaaaacagcg ggccttggcg
    10021 aaaactcgcc agctgctggt cgcttgcggt ctgaacgagg ctatttgtta ctctttcgta
    10081 agtccacaac aatttgacaa attgaatctc acggctgcgg atcccctacg taaaaccctc
    10141 acaattgcta atccactttc tgatgagcaa agtgtgatgc ggacgatgtt aacaggtggt
    10201 ttgttagatg cacttgtcta taaccaaaac cgcaaccaac ataatgtaaa attatttgaa
    10261 ttaggcacag tatttttggc agcagacgtt cctttggctc agcagccaga agaaaaaact
    10321 atgttaggtc tggcgttaat ggggtccttc ccggcagagc attggcagga gaaaccgcaa
    10381 gcggtggatt tctacacttt aaaaggaatt gtggaaaatc tattcgcacg tttaggcgtc
    10441 actggttata gttttaaggc tagtgaattg tcttggtgtc aaccaggcca aactgctgaa
    10501 gtttggttag atgatgttaa gttaggctgg ttggggcatt tacatccgga agcgctggat
    10561 aattatgatt tagcgcagcc tgtttttgtg gctgagttag acatgcatta tattttggca
    10621 cgggtacaac tggtggcggc atatagctcg ctgcctcgtt acccggcggt gctgcgtgat
    10681 ttagcggtgg tggtcccaga tgatactact gctgctgaag tagtaagagt aatacgggag
    10741 gcaggcggaa agcttgttga ggatgttgcc ctgtttgacc agtatcgtgg cgaacaaatt
    10801 ccccaaggac atcgcagctt ggcttttgct gtaacctatc gcgatgctga ccggacgctc
    10861 aatgaccaag aggtaacaaa agtacatcaa caaatagagg cagcgttagg ccagctgtta
    10921 ggtgcaggct tgcgtggtta atattttgcg tggcgtgagc cgtagcagga ataaggtcag
    10981 caaaagaaga ataatacagg gaacaagctt ggggaggtct gccaccatgt cccagttacc
    11041 agaaaaaaac cgtgtatcgg tgaaaattta tgaagaagaa tacaccatgc gcagttctgc
    11101 ttcgccggaa tatatgcgac gcttggctag ttatgttgat gaaaagatga agcaaatagg
    11161 tcagaccaat agccgtttag gcatcaataa agtggcagtt ttaacatcgg taaatctggc
    11221 tgatgaactc tttcgcctgc gcaaagaagt acgtgagctt gaaaatttac tgaaaaagaa
    11281 aaatagcaaa tgatgaactg gtttgacttt ctattagtcg ccattgttgt attttttgcg
    11341 atcgttggct ggcagaaggg tctggtacgt cagcttttcg atctgcttgg cgtaatcgca
    11401 tcttattttg tggccttgcg ttatagtaat caatttgtgt actggctaaa ccagtatcta
    11461 cccctaacat tatggtttaa tcagctattc cccacaccta ctgcctgggg gatagatcta
    11521 ggcgctgttc tgattcgtct ggtgggtttt gccctgcttt ttggtgcagt ttctctggtt
    11581 ttccgtacaa ccggtgccgc tgtccacagt atttttagtc tgcccatctt ggatttggtc
    11641 aatggtttag gtggcttgct tttaggtgcc attagaggtg tttttctggc gttaattctt
    11701 attggtgctg ctaacctgat aggtacgcca tttttagcac gggctgtgac agagtcagct
    11761 atcgctacta ccgtattggc agtgctgccg gtagtttatg agcagatgtt aaggttacta
    11821 tttacagatt ttgtttaagt gccagcgaac acatttcgcc tttactaacc ctggtgtctg
    11881 tggcaagtta atttgtaaat taaagtcttt gtacgggcga actatgttcg cccgctactt
    11941 ttatcagcaa gaaaattata ttgctgataa aaagcagctc attaggcatg cttaaattgg
    12001 ggaaggataa aaagtagcta attcaacaga tagacgtcgg agggatagca tgcgtaatcg
    12061 tgatgtggca gaaatgttag acagagctgc acagattcta gctttgcggg gtgaaaaccg
    12121 ttaccgcatt cgggcttata aaaaagcggc acgtgctgta gccagtttag atgagcggat
    12181 cgatgtttta gtcctagaaa gacgcctgca agatctagaa ggtgtcggcc cggcattagc
    12241 agataaaatt aaagaaattg tcgagacggg taaactagcg atgttggagc gtttagaaac
    12301 cgctaaactg ccccttgcag tgacaaaccg ttctatctta ttagcttcag ccttaacttt
    12361 tagtgcagaa cttttacccg ctttagtggc gcttcccggc gttaagcaga cacaagtagt
    12421 aggccaaaca agacgtcggc aggaaacagt gagcggtctg gagtttgtgc ttactgtcag
    12481 tgacatggtg caggcaaaaa aggcgctgtc tgcttacccc tatctacacc aacagcaatg
    12541 gcaggaagat agatgtctag ctgttcatgc atacggtatg gaaattactt tatatttaac
    12601 cgcggaagag aaatttgtgc aacagttatg gcaaacaact ggttcagctc aacatgtaca
    12661 gaagatagcg gcactggtta aagaaaaatc aggctatgat ctattaactg cggcggataa
    12721 agaaaaaaaa tgttttgcca ctgaggcaga aatctatgcc ttggcgggat tgccggagat
    12781 tccaccagaa ttacgagaag acagcggaga agtggaagcg gcacaacttg gtcagctgcc
    12841 gcatttgata gaggctgcag actacagggg tgatctgcat ctgcatacta actggagtga
    12901 tggcacggcc agtgttgaaa aaatggtaga aactgcagta gagctggggt atgaatactt
    12961 ggcgatcaca gaccattccc aatcactaaa gatagcacgc ggcctttcgc tagaacgctt
    13021 aaaggaacaa aaggccttta ttcgttcact gcaaaataaa tataacattc gcatctttgc
    13081 cgggattgag gctgatatcc tagatgatgg cagtgttgat gcttcagatg aagtattagc
    13141 ggaactcgat gtggttgtcg cctcggtgca tagtgggttt aaacaaagtc gtgaaaaaat
    13201 aaccagacgc atttgtcggg cactgcaaaa cccctatgtg catatcctcg gacatgctac
    13261 tggccgctta ctgggcaagc gtgatcctta tgacgtagac ttagctgagg tgctccgcac
    13321 agctgcggct accggtacgt cgctggaaat aaactcgtcc ccagatcgtt tagatatcaa
    13381 tgaccaggtg gcaagacagg ctaatgaagc cggtattaat gtggctatta acaccgatgc
    13441 acatagtcag ctagagttgg ctaatgtaat actaggcttg gccatggcac gtcgggggtg
    13501 gctggaaaag tcagatgtgc tcaacacaca ttctgcgcag gagctgctgc atcttctgcg
    13561 ccataaaagt aagcagcgcg gctagcgctg ccctaattgc tcgaccatta tttttaagtc
    13621 ggcccggttt ttatgtagat gagacctgac aacccgatca agattatgta atgttactgt
    13681 ctgtaaatct tgaggtgtta gcggttgatg ttctttcttt tttataacac tgaaagtacc
    13741 accactttcc aaaacacctt cttcgacttc attgatatca gcaataccta gtttgcgtaa
    13801 ttcttgcttt aaatcatcaa tattcattcg tactttggcc atgtttgcca taattatctg
    13861 cccatcagca ataataattg tcggattatc ttcaaggaaa agtctgattt tctggttatt
    13921 aagagccagg acggctgcta gaatttccaa taatcccaga gttactagag gaattaaagt
    13981 cggtaagagc ggtaactgta gatccaccat cggtgcggcc gctaccgaac caataattac
    14041 ccctaccaca aaatcaagcg gagaaagttg tccaacctcc cgcttaccca tcaacttaac
    14101 catgattagc gttaccaaat aaatagccag gcttctgcca gctagtttga tagactgcgt
    14161 taatagttct gtcataaccc taatgcacag ctggcggatt ggtagtttgc catttttgca
    14221 ctactttttc acactgcttt agcttttcca caagactgtt gtaagtttgc ttatcagtca
    14281 ttgtaacctg ggcttgcgcc atactggaca ctgtttgctg cagagcttgt gctaactgct
    14341 cgtcagcctg ctgaagttgt tgtcgaacct ttgattcgag ttgctgcagc agctgaggat
    14401 aggactgtgg attattgctg cctcctggtt gatttatggc ttgctgcagt gtttgcactt
    14461 gcaagaaaag agcatcgata ttttgcgcat catttagagc ggtgctagcc tgttgtaaag
    14521 tttgttgaat ggtttgctcg gtttttagtt gctgctgcct cacggaagat tctagttggg
    14581 agacaagttg tgatagttcc tcctgcggtg ttggctgtgg gttaagttgt aattgttgat
    14641 tagttacatt atcagcttgg gctaatgttt ctttcctggt gcggtctagt tcagcaaaag
    14701 tttgttggtc tttttgtaaa ttacccatct tgaaaacccc ctttacttat tgtttatagt
    14761 ttgccgaatt agcccaggat tataaacact atgccaccta tgctaagtaa tataaatcta
    14821 agcaagtatc aaaagagcag gaagtgtttc ttgatgaagc gaacttatac taaacaacta
    14881 gaaaataatt ttttccaccg gcccacagaa gatgtggcgc gtgctttgtt ggggaaagtt
    14941 ttagtgcatc aaggaacggc aggagtgact gcagggagaa ttgtagaggt ggaggcttat
    15001 ctgtatcgca atgatgcggc ctgtcatgcg gcacgaaagc agacgatccg taacaggcca
    15061 atgtttgggc caccgggtac agcttatgta tatttaattt atggccttca ttattgcttt
    15121 aatgtagtga caggattaga gggtgaggga gaagctgtac tggtgcgagc gctcgaaccc
    15181 ttagccggtt taaacattat ggctgaacga cgtggtacgg cagtgacaaa gaaaatggct
    15241 gccgggcctg gtaatctatg tcgcgcactg gcgattacta aggcacataa tggtcataat
    15301 ttaaaccgac caccccttta tctggcggat gatgattttg tcgtcagtga actggtcacg
    15361 accacgcgcg taggaattaa tgtggcgaca gacttaccgc tgcgttttta tattgctaat
    15421 aacccgttta tttcaaggaa gtgatagctt gaaaagagaa attaggctgc tggaatttaa
    15481 taaaattcgc cagttgctgg cggagcaatg tttaacacct ctggggcgag aactagcaga
    15541 agatttggta ccagctcttg atgtaaactt atgccgccgc tggcaaaatg aaactacgga
    15601 agcggcctca ttaatgacac gtcatcaact tggcttagag aatgtgccgg atttacgcag
    15661 cgtatttagc ctggctggtc gcggttcgct gctaggtgag gagcagttag gggctgtgtt
    15721 acggcttctt ctagcagcaa cacgagttaa gctttttttt aaggagaaag aagaatatcc
    15781 ggtgttggca ggtttagcgg cggccatggt gatgctgcca gaattacgcg aagagttaaa
    15841 acaaaccatt gatgaagaag gacgcttgcg tgataatgcc agtcctgaat tagcgcgtct
    15901 gaatagagca ttgcgcaacg gtgaacatga tctgcgtgat cgttttgacc gcttgataaa
    15961 aagccctgct aatcagaagc tattgcagga aagcctgatt acgatgcgtg gtgacagatt
    16021 ggtggtgccc gtacgtgtgg agtatcgtgc ccaggtacta ggagtagtgc atgatcaatc
    16081 tgccagtggt gctactcttt ttgtagagcc cttgtgggca gtagaagcta ataataaatt
    16141 agctgtgctg cgccgagagg ctgaacgcga gaaggagcga attctcgctc gtttttccca
    16201 atgggtgggc ggagaagcgg aaggtctgac cacaactttg acgctgtacg cagaattgga
    16261 ttttatcatg gccaaagggc gacagagtct aaaacttaaa ggtgtgcagc cgcagctgaa
    16321 taatcaaggc tatctaaaaa tcatccaagg tcgtcaccct ttactcatcg gtgatgttgt
    16381 acctattagc ttggaaatgg gtgagcattt acgaacactg gtgattactg gccccaacac
    16441 tggtggtaaa acagttactt taaaaacagt tggacttttt tccttaatgg ctcagtctgg
    16501 gctgcatgtt cctgccctgg agggcacaga acttgctgtc ttccccaaaa tctgtgccga
    16561 tatcggtgat gaacaagata tcagccagtc tctatcaact ttttccgggc atctcaaaaa
    16621 cattgtcgag attctccaga ctttagtgcc gggagcttta gtcctgctag atgaagtagg
    16681 agccggtacc gatcccaccg agggggcagg cttagccatg gccatcctgg catatttaca
    16741 tcagcatggc gccgtgaccg tagccacaac ccactatagt caattaaaaa gtttcgccta
    16801 tttaactccc ggtatggaaa atgcctcggt agaatttgat gtggccacac tgcgaccgac
    16861 ataccaatta ctcgtaggtg ttcccggcgt tagtaatgct tttaacattg caaaacgttt
    16921 aggtttgcct gacgaaatca ttgaccaagg cagagagttc ctctcacagg aagaaaccca
    16981 cctagaagaa gtcgtagcag atttagttgc ggaccgtaaa cgcttggaat atgtttcact
    17041 gcaggcagaa gatgaacgtc agcaggcact tagtttgctc actaaagtag aacgggaaca
    17101 agaagaactt aagctgcgca aagatgaaat attagctaaa gctagagctg aagcactgga
    17161 aatagttgta cgagccaaac gggagacaca gcagctttta aaggagctgc gtaggctggc
    17221 ggcccaatct ctgccaccag cagcagacca aatagaggaa agtgaacgta aactgcagca
    17281 gcttaacgaa gaagtccgtg attttacggc gcccgcacca acagggcagc cgttaacgct
    17341 gcaggaactt gctccagata tggaagtact agtaactagc ctggggcagc gcggtatcat
    17401 tgttcaagta acggaacagc agatacaggt acaaatcggt tccatgcggg tttcagtgga
    17461 tccctctgaa cttacaccga tcgctgctgc agcagccaag gcaaaaccgg agatcaaagt
    17521 aacttcattg tatgtgcggc gtgatgtccc gcctgaaata gatttgcgtg ggcatacttt
    17581 tgctgaagct gcactagagg tagataatta tttggatgaa gcggcaatgg cagaacataa
    17641 agaagtgcgg ctgattcatg gcaaagggac aggtcgcctg cgggccgggc ttaaggatta
    17701 cctgcagggt catccccatg tagcctcgct gcgtatggga catcccaatg aaggtggtac
    17761 aggcgttacg gtggtggaaa taaaataaag acgctgctgt aagcttgatt tctttagcta
    17821 acaaaaagta ccctgctgta tttacaacag ggtactttgt ttattcagct ttgttaattg
    17881 cccttaccat ttccattgcc cttaccatca tcatcatcat catcatcagg tgggggttcc
    17941 tcttgccctg gcggcggctc taccggcggc tcttcctcta ccggtggtgg cggcggcggt
    18001 ggcggtggtt ctactttgat ggttgtggta gtagttgacg attttacgcc ttctgcacta
    18061 atggcagtca gctggtaact ataggcgcca ggcgctaacc cctggtcagt aaaggttgta
    18121 gcagtaacgg ggctgctggt taagctgacg aatgcacctt cgccgggacc tttgcgagcg
    18181 attaggtagc cgctggcgcc atttactgct ttccagctca actcaacatt accgttatca
    18241 ctaacctgtt tagctttaag gtttttaaca tcttctggac gtgaagtgtg tttgtcgcac
    18301 agctcgtcct ctttgggcgg cattaattcc gcatctcttg gtgtccgtcc agaacgtcca
    18361 ggccagcgac catctgtctt gatatattca ggacgattaa gaaatgcttt tgtctccagt
    18421 aattcttgtg ggcaaaactc tgtcggccat aggcctgata ttgagcagac ctccagttca
    18481 acatgcattt cgcaggtgcc tttggggatt gtgctgctag taaaccagtc ggttacaatg
    18541 tgttcatcag ggcaaaactc gcccggacga agtccacttt tgctgcaaat tgttactttt
    18601 cgcaagccat cagggcgttt gaattctact ttcttcagat ccttatgcac agcttttaaa
    18661 atcggattca taaatctagg tgtaacttca aaagagcgat aatcaccttc agcttttggt
    18721 ttaggccagg agtcctgccc catccaaaag acagttacat attgtggtgt ataagccgcc
    18781 atccaggcgt catgagcata ttcactggtg ccagttttta cagctaccgt gcggccaata
    18841 cgcagcgcac tagctgtacc gatgcgaact gtatcaatta aggcactagt ggtaagccag
    18901 gcagcctgtg gtgataagat ttcctcttta ttgggttctt gttcatacaa cacgttaccg
    18961 tcactatcaa caatcttagt aatcgtcgtt agatcggtac gtattccttc atttgctaac
    19021 acggaaaaag cttgggtggt atcccagaca ctgacacctt catttaaacc accaacaacc
    19081 atactcaatc catgatcgtt agtcagagag gtgagcccca ttctttttgc atactctttt
    19141 cctttttcta cccctaattg atccagcaga cgaatagctg gaatgttata tgaattagcg
    19201 atagcattac gcacagtaac taatccccgg aagctcatgt catagttatt gggaaaatag
    19261 ttgcctttaa aaacttttgg tgcatcatct agtactgttc caggtgacgc taatccttca
    19321 ttaaaagcgg gaccataagc aatgatgggc tttaagaccg aaccagcctg catacgtccc
    19381 tttgtggacc ggttaaaaac attaatttca gtctcgtaca aacgtccgcc tactaaagca
    19441 gctacatgtc ctgtggtggg atcagcaact actaccgcag tttgcggttg aataggtttt
    19501 gtttcttcga cgcgaaagct tttacggtag tatttagggt tatctaaaat attaaccaca
    19561 tcttgctgaa ttctttgatc taaggtggtg taaatgtgta aaccactggt gtatagtttt
    19621 gttttaattt cagcgattgt taaatcagca aactctggca gggtggcaag tttttccggt
    19681 gcttcatcca aaataacatg gtcgacaaac cacggatgcg gataggaacg ttccggtata
    19741 cctgacagca caatttcttc tgccttggcg gcttcagctt ctgcttgtgt gatagcatta
    19801 atttccgcca tccgagttaa aatgatttct tgtctttttt tggcttcctc aaaatcacgg
    19861 tatggtgagt atttgccagg taggtttgga atacccgcta acatggctgc ttcacctaaa
    19921 gtcacatcgt tagcactttt gccgaaatag gtttgtgctg ctgcttctac cccatgggcg
    19981 ttaaaatcaa agaaaatacg gttcaaataa aattcaagaa tttcctcttt actatagatg
    20041 cgttccaact gataagataa atatagttcc tgtaatttac gtttaatggt acgttccggc
    20101 gttaagaaag tgttttttac tagctgctgg gtaatggtgc tgccgccttg cattttgccg
    20161 ctttgattag tgaggttata aataaaggca cgaaacatgc cacgtgcatc aaatccggga
    20221 tgatcataaa aacggtcatc ttcaatggca ataaaggcat ccactaaatc tggcgacatc
    20281 tgatcgagcg ggacaatgat tcggttttcg gcgcccatca gtggggtgac gagttctcca
    20341 tctttatcat acataaaaga tgtttgtgct ggttccaatt ctgtgggatc aaagggaggc
    20401 agatcggcca tatagtactt cacggcagcg tatgtagtaa tagctgtaac tgctgcaaag
    20461 acaacaaaga taatggagag aattaaaaat aaacgcttta gtctctgttt gcgcaaattt
    20521 ttattcgcac catttttatt atgattgttt ttcccctctt ttgtttcctt tgacataatg
    20581 ttctcctttc ccgaaagaaa actctctgcc gccattataa catacctatc agattacggt
    20641 aaacggtcaa atcattaggt aattataata ataactgtgc aaaagaaggc agctaggata
    20701 gaattacagt agaatagatg attatggcag agaatgagca aaataggagt ttacagcgat
    20761 attacagcac aacaactgcc aataatagca atacaagcta gtagcgataa gtaacaggtt
    20821 aggaactatt aaaaaaactt aaattatcaa ccacttatgc tttcagaaaa atatggcctt
    20881 gtcctttagt aagcagctac aacagcagca atatagtttt gctatggaaa actgaaaatg
    20941 acagcttttt aatcacattt tgcatttctt ctcatataat gtatggggag tgatgcgcta
    21001 tgctacgacg ccgcctaatt gtcaaacgag aaactagagt cttttttctt ttagcactcc
    21061 ttatttttct aatgatccgc atgttcatgc tgatagaaca taacttgcgc cccaccattt
    21121 tacgctcggc tggcattagg gcaaattata tagcgactga ggctgttaat aaagctattt
    21181 tagaaaatgt agcgcaagat gtagattatc aggatttaat ccttttagtt aaagataaca
    21241 gcggccgtat agtattttct caggtcaata atgtaacaat cagtggcatt atcgctgcta
    21301 cgaatctaca tgctcaggaa gctctgaaaa ctgtggcgcg tgaaaaaata tctattccct
    21361 ttggacaggt gttcaatagt tatctcctag ctaatgtggg gccgagaata ccggtgaaaa
    21421 tatcgcctcg tggcgtgatc aacaccaact tgattgataa attcgagagc gccgggataa
    21481 atcaagtacg tcataaaatc tatttagaag tgcaggcaga aatgcaggta gtgattcccc
    21541 tgatggccga taaaataaat gtctcgacta ccatcctgct ggttgacgct atttatcccg
    21601 gcgaagtgcc gaatacggtt attgaccttc agtatccgct gagcggtcag catgaataga
    21661 gctaaagcat ggtagtcatt actgaataga cggcaaataa aatagcacca aatggtactg
    21721 acaggaccag ggcaccggca gcccaaaaac ctaagccaaa tataaaccct cgcttaaact
    21781 cattatcttt tggcgtccct gctgccgatt gatgcgagct cattgccgta acctcttttt
    21841 ccacttcttc ctgtacagtc ccagtttcat tatcagctgc catctcattt gagattgtct
    21901 cctcaaccac atcttcccgc tcttcgtgca caaactcaat gtcctgatta gttgccagct
    21961 ctgtcattca gcttcctccc cctaaagaaa aatcctttac attctatggt taaaatcaag
    22021 caaacatgcc tgaagagcag ggttgaggtt attgatctaa cttaaatttg cttaatcaat
    22081 tttgtgccga taaggcaatt atgtgcgcta cagttgctgg ctgtaacttt gtaccgtttg
    22141 ccttgggccg caaatccttc tgcaaagcga agtgattcat tagaagtaca aaagaagtac
    22201 aaaaaatgcc cgactgctgt cgggcattag ctagtaccta tatttatatt tccgtcgtct
    22261 aacgataagc aaattttctt ttaatttgct gtaaagcatt aagaataaga ataaaatatt
    22321 agtacttaac tgtaagctta aaaactgtgc taaggaagcc atagctttaa agtgagtgtg
    22381 agaaagctgg atttccaagc ccagacataa aacccacagc actagttcgc cgcaattaag
    22441 gtatagtaat ccttttatta gctggtcctt gctgaaataa aagatcgctg cagtagttat
    22501 atgtaaacaa ataataaaca agttgatata gtctgctgac aaattattat aatgaactag
    22561 ctctacatta tagagaggat ttcctaagct aaagatttgt gaagcctgat ttgattttaa
    22621 aataaacaag gtataaaaag taagcaggat tgtgctgcaa agcattacca gaacggcttt
    22681 aagttgcttt ctttgcattc tatgcccccc gtttagatac attaaaatat aattaccctc
    22741 ataaaatact ttgttattta actgcaggag actacccttg ctgggtttga gctttcccgg
    22801 tcatggtcac taaactgaca ccaactatca caattgcacc accgagcagc tgtcctgtta
    22861 ccagagtttc ttttaaaagc actgctcctc ctaagacccc aaataccggg atgaggttaa
    22921 gaaagctcgt caccgctgct gccccaagtt tctgtaaagc ataaaggtag aaaaagtaag
    22981 ccactgcaga acaaaaaata cctagataca gtaagttaaa agccacttca ggctgtaaaa
    23041 ggacagcatg agcactttgc gggagtgcca gcccactaag cagaaacatg cctgtcgctg
    23101 cctgatataa attaatcacc atactatcat agttttgcag caaaggttca tttaagcgtg
    23161 tgtaaattac ccaagtacag gctgcaccaa aaaccaatag attaccttta agactagtga
    23221 aggatagttc accatcatgt cctgaacgaa tcagcaggta aacgccaata aaagagagca
    23281 atactccgag ccagcgttgg ggattgcttt tttctttaaa tataattgct ccgaccaaga
    23341 catttagcac aggaatggtg gcaataatca gggagcccat gcccgcagtg gtaaaacgta
    23401 agccgttatt ttcaaataag aagtaaataa agttcccaaa gaacccccca gccatgactc
    23461 gcagtaaatc ttttctctgc acagattttt tgtgtgttgc taaaaagagc aggccaagtg
    23521 ctgcacaggc gattagcaaa cggtaaaagg caatctgtgc tggtagaagt gtacctaata
    23581 gcacttttgt actactataa gaaagacccc aaaagacagt agttacgaaa atagcaaaaa
    23641 ctgcctgtag tttctctttt gttttcatat gctcctcttt taccatttaa tactgcatta
    23701 tagcatagga ttacaaagtg ggcaattagt tagtaaaagg ttgaccttcg ccgttaggca
    23761 tactatgatt aaaagggtca ttaagaggga cctggctgtc agggagggga tagggtatgt
    23821 cctgtacaat acggtcggca acacatgagg atctgcgaga tattttagct atctacaatc
    23881 acgctattct taacactacg gctgtctatc attatcaccc acatacatat acagagcgtt
    23941 tagcatggta tgagcaaaaa
//
LOCUS       DASRMS010000220         1281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_61469, whole genome shotgun sequence.
ACCESSION   DASRMS010000220 DASRMS010000000
VERSION     DASRMS010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61469"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1281)
                     /locus_tag="VFC74_10485"
     CDS             complement(<1..>1281)
                     /locus_tag="VFC74_10485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25794.1"
                     /translation="SGIRNAFKELGANISVALSGILNAVAPIVTQIINWLSKIITYIS
                     AFIAMLSGKNTITVAKKQNESYAKSLDKIAKAANKAQLALAGFDELNNLTTQKDSGGS
                     GAGISPGSLFEEKEVDSILPGFVKNWFEKLKGAIQDGDWRSVGKTIAEGLNAAVNSLD
                     DWIDNTLEPKGMKMASNLAQGINGFFEKFDSYKLGQSIAHGINAAVGTARTFLKETDF
                     QKIGASIMEFINGALDDIDWGEVGATASAGVGAILDAIDGAISELNWNSLATAVEDAI
                     MGIDWGNLLGKTLRIALRAALFGTEATAPGGSFFKSVLGDLPGEAKELAQETITGWFD
                     GLKPKTPFGQWCRDYLLTPFVDAIKKWFQISSPSKVTMQLGGYVSAGFLDGIKDKLKN
                     IAAWVQNNIFMPVMNAVKKAFGVAGNVASRFLEIG"
BASE COUNT          318 a          353 c          238 g          372 t
ORIGIN      
        1 cctatttcta aaaacctgct tgcaacattt ccggcaacgc caaacgcttt ctttacagca
       61 ttcataacag gcatgaaaat gttgttttgc acccatgccg cgatgttctt aagtttatcc
      121 tttatgccgt caaggaatcc cgccgaaaca tacccgccaa gctgcatagt tactttagat
      181 ggcgaggaaa tctgaaacca tttttttata gcgtcaacaa acggtgtgag aaggtaatcc
      241 ctgcaccact gcccaaacgg cgtcttgggc tttaacccgt caaaccaccc tgttattgtt
      301 tcttgtgcga gttcttttgc ctcgcctggt aaatctccaa gcacactctt aaaaaagctc
      361 cccccgggag ctgtcgcctc tgtgccaaac aacgctgcgc gcaaagcaat tctaagagtt
      421 ttccccaata gattccccca gtcaataccc ataatcgcat cttcaacagc ggtagcaagc
      481 gagttccaat tcaattcaga tattgcaccg tcaatagcat caaggattgc tcccacacca
      541 gcggaagcgg ttgcgccgac ttcaccccaa tcgatatcat ccaatgcacc attgataaac
      601 tccatgatgc ttgccccaat tttttgaaag tcagtctcct tcaggaacgt tctggccgtt
      661 ccaaccgccg catttattcc atgtgctatg ctttgcccca gtttgtacga atcaaacttt
      721 tcaaagaatc cgtttatgcc ttgagctaaa ttgctcgcca tcttcatgcc ctttggctca
      781 agcgtattgt ctatccaatc gtcaaggcta ttaacagccg cattaagccc ttctgctatc
      841 gttttgccta ctgaacgcca atcaccatct tgaatcgccc ccttaagctt ctcgaaccag
      901 tttttaacaa aaccaggcaa aatagagtca acctcttttt cttcaaatag actgcccgga
      961 gatattcctg cgccgctgcc gccgctgtcc ttctgggtag taaggttatt taactcgtca
     1021 aatcctgcaa gggcgagctg tgccttgttc gcagccttag ctatcttgtc aagcgattta
     1081 gcataacttt cattctgctt tttagctacc gttattgtgt tcttccctga tagcatagct
     1141 ataaaggccg aaatataggt tataattttg cttaaccagt tgatgatttg cgtgactatc
     1201 ggcgcgaccg catttagtat gcccgacaag gcaacgctta tatttgcgcc cagctctttg
     1261 aaggcgttac gtattcctga t
//
LOCUS       DASRMS010000221        32417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_400292, whole genome shotgun sequence.
ACCESSION   DASRMS010000221 DASRMS010000000
VERSION     DASRMS010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 32417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32417
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400292"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..989)
                     /gene="mutM"
                     /locus_tag="VFC74_10490"
     CDS             complement(165..989)
                     /gene="mutM"
                     /locus_tag="VFC74_10490"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HZK25795.1"
                     /translation="MPELPEVETIRQGLEQVLPGRVFASIDVHYAGSIKAPSVAEFTA
                     QLPGHQVAATGRRGKYLHLYLDDLSVIVIHLRMTGKLIFSTTALARDKHTHVVFNFTD
                     GTCLAFHDVRKFGTIYWLTQERLCEIKGLACLGPEPLGQDFCVEYMVSKFAKRKGSIK
                     ALLLNQEFVAGLGNIYADESLHCARIRPDRAAASLTTEEIKTLYQAVCEVLTEAIKCR
                     GTTMSDYRDASGGYGQFQERLRVYQRTGQACLQCGTSIERIVVAGRGTHFCPSCQK"
     gene            complement(1006..3642)
                     /gene="polA"
                     /locus_tag="VFC74_10495"
     CDS             complement(1006..3642)
                     /gene="polA"
                     /locus_tag="VFC74_10495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HZK25796.1"
                     /translation="MKDKQKFLIIDGSSLVYRAFFALPLLQTKQGLYTNAVYGFTTML
                     LKVLEDEAPDYVVVAFDKSKKTFRHEKYSAYKGRREKTPQELSEQFSYVREVLAALQI
                     PVEEMAGFEADDLIGTLAVLGKKHQLTPVIVSGDADIFQLVDIPAEVIFTRRGITQVE
                     RYNEETLKNKYGLTAQQFIDYKGLKGDASDNIPGVPGVGEKTAQHLLAQFGTMAGVYE
                     HLAEIKGKLRSRLEDNREQAYLSKYLATIVTDVPLNFVPAHYQRQAPQLEGLRRIFTQ
                     LEFNSLLDKLPDSESRAAEQITTEAYRQVTLNEWREFFPALAEESAVAITPLPLDAAW
                     QETLKGLAVATSVETIFLPLTEQRDLAVVLEMLLDSSFTTLTYDSKAWINICWQLTGK
                     EPTAPLFDAALAAYLLDPLENGYPPQKLAQHYLQRHLPQAPGKKETDTTCESNYLCAA
                     VRTLFDLYPVLLAKLQEFDLAALYHQLELPLAAVLARMERQGVAVDQATLAALRDEFS
                     QRIAVLQQDIFELAGEEFNLNSPKQLAVILFEKLGLPALKKTKTGYSTNAQVLEELAP
                     HHEIVEKILQYRTLIKLLGTYLAGMAKLINPQTGRIHTTFKQTVTATGRLSSAEPNLQ
                     NIPIRLEEGRRIRRAFVPGTEGKVLLSADYSQIELRVMAHISGDQVLISSFKAGEDIH
                     LRTASEVFDVPPEEVSKALRERAKAVNFGIIYGISDYGLSRQLGITRQEAKSYIERYF
                     ERLPGVHDYMQRIVAEAKQNGYVTTILNRRRYLPDINHRNFNKRSFAERTAMNTPIQG
                     SAADIIKLAMLNVERQLQPWRDKARMILQVHDDLVLEVDEAILPEVVKIVRREMEEAI
                     QLSVPLLVEVKVGHNWAEMQKI"
     gene            complement(3639..4232)
                     /locus_tag="VFC74_10500"
     CDS             complement(3639..4232)
                     /locus_tag="VFC74_10500"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HZK25797.1"
                     /translation="MTCEEVIESMAEYLDELLPAERKNAYERHLQTCALCRKLTTETK
                     SAVQWVQQAEKLTPPPNLRSAVLSTLKQEKQANRTHLAPGLVQIMAAAAVFVLLIAGN
                     LLPWAADGTPEIRGYDGSAVMFDNSSPPAEQFSGAESEEQVIKKADESAVVAAQRQTK
                     RPAQPRFSPRLVVNLVLSTVIIILLMMARKKRRGLLP"
     gene            complement(4229..4858)
                     /locus_tag="VFC74_10505"
     CDS             complement(4229..4858)
                     /locus_tag="VFC74_10505"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HZK25798.1"
                     /translation="MSRLPEREKILVRRCQAGDLLAFDQLMQRYEKKVYALCYRMSGN
                     PEDTADLAQEAFLKAFRALPLFKGQAQFSTWLYRIAANTCLDEQRKRKKYQPMLSLDQ
                     PISTKDGELQRQIPDDAPDPLTATIRRELQFEIQTLLTQLPPAQRAVLALRDLDGFSY
                     EEIAAILQVKMGTLKSRLNRARASLRELYLQKEEQIQPEHIQQRKGAKK"
     gene            complement(5006..5659)
                     /gene="rpe"
                     /locus_tag="VFC74_10510"
     CDS             complement(5006..5659)
                     /gene="rpe"
                     /locus_tag="VFC74_10510"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HZK25799.1"
                     /translation="MVKIAPSLLAADFSRLAEEVKKVETAGADWLHFDVMDGHFVPNL
                     TMGPQLVKSLRHTSQLFFDVHLMIEQPDRFLEAFVAAGADLLTVSAEACVHLHRVLQM
                     VRKLGVKAGVALNPATPLSVLEHVLDDLDLVLIMSVNPGFGGQKFIPAMLPKIEKLKN
                     MIGQRKIEIQVDGGINQTTASLVQAAGATVLVAGTAVFGEQNVVQAIKMLQTETKVV"
     gene            complement(5662..6528)
                     /gene="rsgA"
                     /locus_tag="VFC74_10515"
     CDS             complement(5662..6528)
                     /gene="rsgA"
                     /locus_tag="VFC74_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HZK25800.1"
                     /translation="MIIRAVGGFYDVKTADGEEYRCRARGRLKKTNTGIYVGDYVRFT
                     RLSDQEGVLEEIEPRRTHLLRPQVSNVDQVIIVCAPQNPPLSLQLLDRLLVLAEAQQL
                     RSVICVNKEDLRLDDLADFHHLYSSLAGYTVLFTSAKYNQGIDQLRQELNERVSVLAG
                     PSGVGKSSLLNRLQPGLELRTAAVSEKSKQGRHTTRHVELLSLDSGGLIADSPGFSQL
                     ELIGISSNNLAFYFPEFFQYIVDCRFTSCTHQGEPACAVREAVGAGKLTHSRYDNYLF
                     FLHEIKQQERSY"
     gene            complement(6557..8380)
                     /locus_tag="VFC74_10520"
     CDS             complement(6557..8380)
                     /locus_tag="VFC74_10520"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HZK25801.1"
                     /translation="MIGKILGNRYQIVEKIGDGGTAFVYKGMDNLLNRHVTIKVLRPE
                     YVSDQDFVRRFRREAQAAASLSHPNIVSIYDVGYEDGIHYIVMEYIHGRSLKELITEL
                     ERLPVRMAVEYALHMAQALEHAHKHGIIHRDVKPHNILISEDGRIKVTDFGIAQAVTA
                     STITYNNGSILGSVHYFSPEQARGGLTDEKSDIYSLGIVLFEMLTGQVPYQGQSPVSI
                     ALKHLQEPFPKAKALNPEIPDSINQIISRAVEKEPEDRFPSVQAMASELTAWLQGREG
                     RRSTMEPVPITGGKGMSKGQHKTAAKRPRARTAAIAGAVLLLALFAFGVVRLLQVFYV
                     PEVEVPTVEGETLQKATIILEEAGLTAALADQIPSDTVPAGAVIRQDPAAGKKVKEGR
                     KITLTLSTGPQLVQVDSVVGKMRREAALLLEENFVVEINEVHSVEAPNTVVSQDPGAG
                     NRIAKGSTVTLNISKGGQPFKLKDLRGLSLEEATTWLELFNLNIRHVAEENNEDIGAG
                     LIYSQFPEPNEMVQAGDFVDLYLSKGPKELPTPELEQYPIEINTEDIPAGETITVVIH
                     DETGDRTETYTSDGQPITIIGFGSGTVEVKWNDQVDTKSFP"
     gene            complement(8410..9162)
                     /locus_tag="VFC74_10525"
     CDS             complement(8410..9162)
                     /locus_tag="VFC74_10525"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HZK25802.1"
                     /translation="MKRLQAVGISHRGKVRSNNEDRYLIDDDEAIAIYAVADGMGGHA
                     AGEVASSMALEVVHNFSADRRQEFLMAADNRQTVRPLLEEMLAQANAEVLNAGTTNPD
                     YAGMGTTLTMLMVIAGQSWLAHIGDSRAYLLREDDLLPLTDDHTLVSQLVRTGQISAE
                     EQDGHPQRNILTRALGTDEVAVFDLLPLTLLQNDGLLLCSDGLYGLVSNAEIKHIMTQ
                     DNAPLEKIQELVALANEKGGTDNITAVLIYNI"
     gene            complement(9159..10214)
                     /gene="rlmN"
                     /locus_tag="VFC74_10530"
     CDS             complement(9159..10214)
                     /gene="rlmN"
                     /locus_tag="VFC74_10530"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012159288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HZK25803.1"
                     /translation="MQKIDLLSMTPTELTGFLRDLGEPAFRTKQIMEWLWQKDAASFM
                     AMSNLPLALREQLGKRAVIGTIAVAAQESSIDGTEKILFVLPDGQTVETVVLPYKIGY
                     SACISTQVGCRMGCRFCASGLPGFVRNLKMGEIMAQVLYVRRTLRAQGRELKGMVLMG
                     SGEPLDNWAATIPFLEAVQDPQRLGMSLRHLTLSTSGLAPKILQLATLGWSLNLAVSL
                     HAPNNEVRNRLMPINKKYPLEVLLPACDEYAQKTGRRVTYEYILIEGVNDEKQHAKQC
                     AHLLAGRLCHVNLIPFNTVKELPWQASPPEKIHTFKAILSAKGINVTIRRKMGADIAA
                     ACGQLRNNYCGEPKNEG"
     gene            complement(10377..11735)
                     /gene="rsmB"
                     /locus_tag="VFC74_10535"
     CDS             complement(10377..11735)
                     /gene="rsmB"
                     /locus_tag="VFC74_10535"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF011494.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HZK25804.1"
                     /translation="MTAKKTLNAREASLLALNKIEEEDAFANLALAAVFAAHTMDVRD
                     KRLATEITYGVTSYRLTLDWLISQIAGRPVTKLDRPVLQALRIGFYQLFYLDRLPAPA
                     VVHSTVELIKKSKKRALAPFVNGVLRGFLRKKDALVWPNPELEPLAYLTIRYAHPEWL
                     VKRWLKRFGREAAEKLLAANNQTPPPSARVNTLRTSRDQLLMRLGEENIQAIASKVVP
                     DGILVSGGGHLTAAAAYHEGLFQIQGESAMLTSHVLNPPTGAKILDGCSAPGGKTTHL
                     AQLMENAGEVVALDLYEHRLALVQANASRLGVKIIQTASLDVRSILPQHFGMFDHVLL
                     DVPCSGLGVIRRKPDIKWRRQEKDIAALAAVQREILDAAAQVLKPGGTLVYSTCTNEP
                     EETEDIIKAFLHRQQNFAAADLTPYLPLSWHEQIGTYGIQLYPHLQEVDGFFIAKLQK
                     KA"
     gene            complement(11737..12417)
                     /locus_tag="VFC74_10540"
     CDS             complement(11737..12417)
                     /locus_tag="VFC74_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HZK25805.1"
                     /translation="MFLPDFTWLIVLPALFFAIWAQSQVQSAFQRNTRLQARSGLTGA
                     QVSRQLLNEAGLTNVQVELGQGQLTDHYDPRQQVVRLSPAVYQSSSLAALGIAAHETG
                     HAMQHAEGYLPLAFRNNIFPVANLGSRLAFPLFFLGFLFRGGLLMEIGIMLFGFAVLF
                     QLVTLPVELNASSRAIALLDNRGFLTSAEIGPTREVLRAAALTYVAAVAVSITQLLRL
                     LILRNRRR"
     gene            complement(12454..13197)
                     /locus_tag="VFC74_10545"
     CDS             complement(12454..13197)
                     /locus_tag="VFC74_10545"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HZK25806.1"
                     /translation="MIRKRLYLGLLAGAMVLFAGVLGYLWLSFFQHDAGFFRYLMIFA
                     VIIILGGLAFIALGFSGIILSLVSDHDLVWLHKPINFTVSLLYPVVIWIGKMFKIAQE
                     RIQCSFVEVNNQLVKAKRGQLAPERLLVLLPHCLQMTDCDKRITITTDNCARCGRCPI
                     GGILNLCDSYGCHVQIATGGTLARDAVKKLRPKAIVAVACERDLASGILDCIPLPVLG
                     VTNERPHGPCFNTQVNLLAVEKAIQFFTT"
     gene            complement(13226..14158)
                     /gene="fmt"
                     /locus_tag="VFC74_10550"
     CDS             complement(13226..14158)
                     /gene="fmt"
                     /locus_tag="VFC74_10550"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011878046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HZK25807.1"
                     /translation="MAKTTLVFMGTPEFAVPSLQALAGSFHILAVVTQPDRRAGRGRK
                     MQSPAVKKAALALEMPVVQPEDLRQADFAAWLAGLQPDYLVTCAYGKILPLALLALPR
                     LGALNVHASLLPYYRGSAPIHRAIMNGDQESGITIMHMDQGMDTGDMILQQALPITAA
                     DTTGSLHHKLADLGAKLVVEAIIAINNGQAQRRIQDNQLATYAPPLTREDEQIDWCQS
                     SQVIHNQVRGMNPWPGAYTELNDQRLKIWEGRPVAKHGNPCGAVLAVDQEGLLVATGD
                     GAYLITKLQPPGKKTMLAADFLRGHHLALGTILS"
     gene            complement(14179..14643)
                     /gene="def"
                     /locus_tag="VFC74_10555"
     CDS             complement(14179..14643)
                     /gene="def"
                     /locus_tag="VFC74_10555"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003517641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HZK25808.1"
                     /translation="MALRQIRKNGDPALRQKAREVRKITPAITKLLNEMAETMYDAQG
                     VGLAAPQVGISKRLVVIDVQDDRGLLKLINPKIIDRSGKETAIEGCLSFPGIAGEVER
                     AAQVTVEALDETGTKITIEAEGLLARACQHEFDHLEGVLFVDRATRFIEQEE"
     gene            complement(14676..16868)
                     /gene="priA"
                     /locus_tag="VFC74_10560"
     CDS             complement(14676..16868)
                     /gene="priA"
                     /locus_tag="VFC74_10560"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HZK25809.1"
                     /translation="MEIFADIIVSIHNLPLEHPFTYLVPAKIKLVVGSRVLVPFGNRK
                     LVGYCVRLHRELPGESKGKIKEIIRALDEEPLLTAELVALATWGAQRYLCQRIKFLEA
                     MVPSSGRTTKGLRAKKTKTAVLLAAGQAAKATGKKQQLALQLLSRQGALALTQLADAG
                     VGAATVRSLEKKGWLEIKEQIVRRDPLADTAVEAQTALSLTNAQREALAYINQSEELK
                     PVLLFGVTGSGKTEVYLRAIAQRLSAGLASIVLVPEIALTPQMTERFVSRFGDRVAVL
                     HSRLGIGERYDEWCRIARGEALVIIGARSAVFAPVKKLGLIILDEEHETTYKQEESPR
                     YHARELALWRAQWHGAKVILGSATPSLESYHAAEGGNYQLLRLPERIAQRPLPPVEVV
                     DMREELKDGHKSIFSRPLITALQETVAAGKQIILFLNRRGYATFVLCRECGHVMRCPA
                     CNVALKYHAAATTLRCHYCDHHEPYPNTCPVCSGRYIRHFGTGTQKVEAELLKQFPDY
                     RVLRLDADTTTHKGDHQKILTAFKQQKADVLVGTQMIAKGLDFPNVTLVGVITADTAI
                     NLPDFRAGERTFQLLTQVAGRAGRGAAGGKVVVQTYTPEHYAIVAAKTHDYESFYAEE
                     SQARAALGYPPYGFLLRLLVSGQQEGAVVDSCSFLVSTFAEDIELLGPSPCPIEKVRN
                     RFRWQLVVRGDSLEPLLAAAKKAAAAFLETPLAGSVRLALDVEPQSLL"
     gene            17059..17670
                     /locus_tag="VFC74_10565"
     CDS             17059..17670
                     /locus_tag="VFC74_10565"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HZK25810.1"
                     /translation="MTKTFFLLIRENRSWIILAILFFVMGLVSAYSALGQEPAFLQLL
                     EESLGALSELGEEIFSGSPLTGVFVLFGNNLIATMQIIFLGIFLGLPSLSAAIANGAV
                     LGFLSFQLVQEGIAPLPFLLSGVLPHGIFELPAFFLCVALGLKLGYHVIFPLPELTRR
                     QTFQKIFKEIGIALPYILGLLAIAAVIEVFITPLLLGSLMGLI"
     gene            complement(17759..18964)
                     /gene="coaBC"
                     /locus_tag="VFC74_10570"
     CDS             complement(17759..18964)
                     /gene="coaBC"
                     /locus_tag="VFC74_10570"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008409970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HZK25811.1"
                     /translation="MLSGQSIVVGVTGGIAVYKAAELCRLFIKAGAEVRVVMTEAAKE
                     FVTPLTFQTLTGNRVYDQLFIGSDRFSVEHVGLAQEADLIVLAPATANTIGKIANGIA
                     DNLLTTTVMAAKCPILLAPAMNTHMYENPIHLANLKKLESYGYALVGPDQGELACGDS
                     GRGRMSSPQDIFAVACRLLRQKKVWQGRRLLITAGPTQEALDPVRFLTNHSSGKMGYA
                     LAQAAAELGAAVTLVSGPVMLPPPYGVDLVSVTTAQEMYDAVLARFASTDVVISAAAV
                     ADYRPSTSSEQKIKKSGELVINLTRNPDILAALGQIKKQQILVGFAAETENLRENALA
                     KLERKNLELIVANDILQEGAGFAVDTNIVRLYYRDGREQQLGKLAKREVADILLAAIS
                     ELLATAEAS"
     gene            complement(18976..19173)
                     /gene="rpoZ"
                     /locus_tag="VFC74_10575"
     CDS             complement(18976..19173)
                     /gene="rpoZ"
                     /locus_tag="VFC74_10575"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013623926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HZK25812.1"
                     /translation="MIYPSIDTLMTKVDSKYTLVIAAAKRARMLNENHPALIKSNATK
                     QVSIALEEIAADKIGYERIKK"
     gene            complement(19170..19787)
                     /gene="gmk"
                     /locus_tag="VFC74_10580"
     CDS             complement(19170..19787)
                     /gene="gmk"
                     /locus_tag="VFC74_10580"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011590947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HZK25813.1"
                     /translation="MPRPGLLIVLSGPSGAGKGTVCKALLEKDPKLVVSVSKTTRQPR
                     CGEKNGVNYCFVTEEEFKAAIAQQDFLEYACVYDHYYGTPRVAVEEKLAAGNNVILEI
                     DPQGAQKVMKSYPDGVFIFLLPPSGTELRKRIINRGTESTESLQKRLAAASQEIKRAS
                     AYNYVVVNDQITSASERIHAIIQAEQLRVVRNQSLIQILAEEVKG"
     gene            complement(19809..20078)
                     /locus_tag="VFC74_10585"
     CDS             complement(19809..20078)
                     /locus_tag="VFC74_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003458433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF370 domain-containing protein"
                     /protein_id="HZK25814.1"
                     /translation="MQIKLINIGFGNIVSANRIIAIVSPESAPIKRVIAEARDRGMLI
                     DATYGRRTRAVIVTDSGHVVLSAVQPETVKHRLHTRETPAADDED"
     gene            complement(20204..21085)
                     /locus_tag="VFC74_10590"
     CDS             complement(20204..21085)
                     /locus_tag="VFC74_10590"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HZK25815.1"
                     /translation="MIGSMTGYGQAENSVDEITRIKVEMRSVNHRYLDISIRMPREIQ
                     TLEERIRRRVQQKLGRGRVDIFISWHDETDAAVNVTVDRPLVRAYQRALQEVSELYAI
                     KESASLQLLAGFPDVLRVEKPQVDLDEVWERLAFVLDQALNNLIEQRQAEGARLLEDF
                     KLRLTKVAQAAELVETRTPLIIEEHRSKLEERLNEVLGQADYDPARVLTEVAIFADRS
                     NVTEELVRLTSHLTAFQATLKETGAIGRKLDFLTQELFRETNTIGSKANDYEVAKLVV
                     EMKTEIEKIREQVQNIE"
     gene            complement(21182..23920)
                     /locus_tag="VFC74_10595"
     CDS             complement(21182..23920)
                     /locus_tag="VFC74_10595"
                     /EC_number="7.2.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014795118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005388 - P-type calcium transporter
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-translocating P-type ATPase, SERCA-type"
                     /protein_id="HZK25816.1"
                     /translation="MNKLPWSQLPTAELEQHFQVDALKGLSSSEAAQRLKENGHNSLA
                     EKKKVPLLVIFLWQFRDFMVLVLLGATLLSALLGEYSDAVVIIGVVLLNAILGFIQEY
                     RAEQSLEALRELTAPTAKTLRDGVRYEIPAEELVPGDIVLLEAGDRIPADIRLCEVRQ
                     LLVNEASLTGESEPVTKISDPLSEEVTVLGERFNMVFMGTMAVGGHASGVVVATGMAT
                     EMGRVAHLIQDAENEDTPLQRRLEQLGRYLVIGCLLICALVVVMGVAQGLPAYNMLMA
                     GISLAVAAIPEGLPAVVTIALAIGVQRMVRKNAVVRRLPAVETLGCATIICSDKTGTL
                     TQNKMNVQEIWAGGRHYRVAGEGYSPQGQFLSGTRQIKPGKDAGLMLTLTASVLCNNA
                     QLLKGNMEIQPLWRGKTASWEIQGDPTEGALVVAGARAGLWREDLERSMQRLGEIPFD
                     GARKRMSVLYSGTVGQILYVKGAPEVIMDKCSQIFYHGRVVEFSHSLRQEVMQQNETM
                     AALALRNLAVAYRPLTNTQTVSEELENKLIFVGILGMIDPPRPEVLGAIQKCSAAGIK
                     TVMITGDHKTTAMAIARKLHLLPLQGQVLTGRELDELNDKELEKAVTNTYVYARVAPE
                     HKLRIVRALKRRGHIVAMTGDGVNDAPAVKEADIGIAMGKSGTDVTKEAAALVLTDDN
                     FTTIVSAVEEGRGIYDNIRKFIRFLLACNTGEILTMLVAMLVGLPIPLKAIQILWINL
                     VTDGLPAMALGVDPVDSDVMARLPRSPRESIFARGLWQKIVGRGTVIGLTTILIFAWA
                     LQQGWELDEARTMAFTTLIITQMVYVFDCRSEQRSMLQIPLFSNTWLVAAVASSMGLL
                     LAVLYHPLLTAIFLTRPLQSEQWIIILTACFLPVLINALYSLLKSAFAPRVVIMKK"
     gene            24074..25804
                     /locus_tag="VFC74_10600"
     CDS             24074..25804
                     /locus_tag="VFC74_10600"
                     /inference="COORDINATES: protein motif:HMM:NF017633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT RNA binding domain-containing protein"
                     /protein_id="HZK25817.1"
                     /translation="MAYDGLTMACVQQELKHSLIGARIEKIYQPSNREIVLHLRTREA
                     ALMLLCSADPQLARVHLTTAKPANPAVAPAFCMLMRKHLTGARLLDIEQDQLERVLTF
                     VFRSYDQFGDNTKKRLVCEVMGKHSNIILTMPNEQEIPQVLGSIKTVDETMSRLRVVM
                     PGEIYFPPPAQDKLNLFDFTEEELTAALLQAENKAADNALVQAVGGLGRELAREIIRR
                     AAANEPAHPLAMVRPLTIELQRLAAAFKNGKLTPCLRKLEDQKPEFSLLAPTSAQQIE
                     LTFYATVNEALDAYYTAVVQELQTNEVRQQLLHTINKHLTRSEKKQYLQKNELQEMEG
                     ADKYRIWGEMLTASLHQLRSGMKEAHVINYYAENIEKLKIPLDPSLSPQANAQRYFKR
                     YRKLSDGKKYLRQRLKATYSQLNYLESLLVAAQNADLPALLEIREEMESDGLIRSRSH
                     KKRVTAPPSQPLHFLSPDGIEVLVGKNNRQNDQLTMRLAASADVWLHVKDVPGSHVII
                     RHAEPPTSTLLFAAALAAHYSKAAASANVPVDYTQVRHVKKPRGAKPGMVIYDHHNTV
                     YVTPSAGHKQ"
     gene            complement(25869..26177)
                     /locus_tag="VFC74_10605"
     CDS             complement(25869..26177)
                     /locus_tag="VFC74_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25818.1"
                     /translation="MAQESSLFAKVEEQEVKGEIFQVTHRILHIPREVYLVVLKDHEK
                     PFSEVGAQIFVEEYLKWLGEENGIIGMVRLAEEDGQVILDAAIRYLINPLEGPTCCTE
                     "
     gene            complement(26222..28114)
                     /locus_tag="VFC74_10610"
     CDS             complement(26222..28114)
                     /locus_tag="VFC74_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HZK25819.1"
                     /translation="MQSRRIGGRGSRYLTEEEKQNKPEVTSQLLKRIFSYLLPYWKQL
                     CVVIITIMISAVLGVYPTLLTGRIIDEGLLGKNLKTLLLLIIASFVLTIASNLVNVLQ
                     SYINTWVAQHIIYDMRNNMFTHLQKMSHNFFTTNKQGDIITRMTSDIDGVRNVIVNTL
                     TSIIQNIATLIVALAAMYQKNWILATLAVVLVPLLTIPTKRVGKTRWSLALESQKYND
                     QINQILNETLSVSGQMLVKLFTKEKTEYDKYEASNREMVNISIKESMVGRWFRVVLNT
                     FTSVGPMLIYLGGGMIIIFNPHSNLTVGDITVMVTLLSRMYRPVNSLMNVQVDVIRSL
                     ALFSRIFEYFDIQPEIENAADAIIPSAWKGKLEFDNVVFSYSSDVPVLRGITFSVPAG
                     KSVALVGASGAGKSTIINLIPRVYDVQGGSILLDGTDIRKLDLNYLRRKIGMVTQDTY
                     LFNATLQDNLLYAKADATQEELEEACRKANVHDFIMSLPDKYETIVGNRGLKLSGGEK
                     QRVSIARVILKDPPIIIFDEATSSLDSISENLIQDAIEPLLKGRTSIVIAHRLSTILT
                     ADEILVIKEGVIKERGVHSELVIKEGIYTELYETQFRRAIEEHEERKISRIATTQDNI
                     VQLMKE"
     gene            complement(28202..28762)
                     /locus_tag="VFC74_10615"
     CDS             complement(28202..28762)
                     /locus_tag="VFC74_10615"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HZK25820.1"
                     /translation="MSKEHSDLLNKFFRLQRLLGRFYHERSKKHGPMGNIHRGQGRIL
                     ALLKLKPEISQKELSHLLDMRPQSLGELLEKLEKNGYITRTPSEEDQRVLIIHLTDAG
                     KELSVLNEQQADAEDLFKSLSVEEQVALNDYLDRIVAVLEQKFQDDGHQPESAGSSSS
                     YGTKHFHSTGSHHHHRHKRFKGGGPS"
     gene            complement(28938..29579)
                     /locus_tag="VFC74_10620"
     CDS             complement(28938..29579)
                     /locus_tag="VFC74_10620"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HZK25821.1"
                     /translation="MMDIQLVRTEFKLPNKIVYLDVVDDIEALITDLSDADAVPCWAE
                     IWPSARALARYIDKNLNLLDKSVLELGSGLGLPGTICGIRGGQVMFSDYHQAAVELSL
                     HNAALNGVKAEGFLGDWRAFTLQQRFDLLLGSDIFYDPQLNAYLLTIFKDNLKAGGQI
                     LISHQRRLPTYHFVEQLKRQLKLQEVRIDCIEREEESVYGQFFVSIHHLQPAP"
     gene            29616..30089
                     /locus_tag="VFC74_10625"
     CDS             29616..30089
                     /locus_tag="VFC74_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25822.1"
                     /translation="MFRWRRIKSTREVWRKGEKLRRAGKTKAAEELLLKAAASTSPLD
                     KHYAYLKLIQLYQELNNAGDNRQQDLIVTCQKDIALFPDFYEAWLIEYFNNVPTPYFP
                     SFSVLAEIYEREGRLQETIDLCELAIGYNLQETRGEDFPEKLERLYRQRDLQQEK"
     gene            30150..30335
                     /locus_tag="VFC74_10630"
     CDS             30150..30335
                     /locus_tag="VFC74_10630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25823.1"
                     /translation="MMTTFHWIHIIVGVWLAFVNFMPILPSELLMLNNAIIGIVVTLY
                     NAYFLFAKNNTDVKQKS"
     gene            30392..30532
                     /locus_tag="VFC74_10635"
     CDS             30392..30532
                     /locus_tag="VFC74_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25824.1"
                     /translation="MSEFKNDSRESDFASVAIKYGAWLLGLIIVLYFVSRNVFPFIQS
                     LF"
     gene            complement(30529..31176)
                     /locus_tag="VFC74_10640"
     CDS             complement(30529..31176)
                     /locus_tag="VFC74_10640"
                     /inference="COORDINATES: protein motif:HMM:NF018626.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HZK25825.1"
                     /translation="MLRRKSSSLIIITLVIVVIVVLQWIKVPGESLVEKKPSNQRITA
                     DNILLGARAEAKKRVRYNAAYAVIAYPGGDISQTQGACTDVVIRALRNADIDLQVLIH
                     QDMQANFALYPQDYGLDAPDPNIDHRRVRNQMVFFSRFGQELPLTVADQLETWQWGDI
                     VSWRCADGQLHTGIVSDRTNRQGIPLVIHNNSVTKEEDCLQRWDIIGHYRYPAVF"
     gene            complement(31303..31467)
                     /locus_tag="VFC74_10645"
     CDS             complement(31303..31467)
                     /locus_tag="VFC74_10645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25826.1"
                     /translation="MNHLEYMVVYRTLQGQQAGIYKEMTQKQLDRLLHKLKQEGCVVN
                     SVEIKRRCFR"
     gene            complement(31557..32303)
                     /locus_tag="VFC74_10650"
     CDS             complement(31557..32303)
                     /locus_tag="VFC74_10650"
                     /inference="COORDINATES: protein motif:HMM:NF019976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HZK25827.1"
                     /translation="MWLYLSLVLAVIYLALRLPALVSGLRRRKTKQMLAQLDHHDYYI
                     LHNLILQITPGRTLLIEHLVISTHGIFVIKLCEKIGRIEGAEHGDFWVKILQYKKRFP
                     NPLQQCKVQTKALRELFSNARVPITPIVLFNIFTEVKADVAAHLIDVEDLLPTIKSYE
                     KVYLKAEQMQAIISMVEGKRAKKVTTQARIYDIRELLQERNRLVKADICPKCNSALMI
                     REGKHGTYKSCSKYPKCKFLYAISAQRKEL"
BASE COUNT         8703 a         7996 c         6701 g         9017 t
ORIGIN      
        1 cgggtcggga ctttgcctct ggcttccttc agattccacc tcacgatgga cacccttgcc
       61 tttggctaat ggttcccact gcaaagccca tagcggactt tcaccgccaa gctaccgccc
      121 atgccgggca caccaaaaaa agggcgaact aggttcgccc ttcattactt ttgacagctt
      181 ggacagaaat gtgtacctcg tccggctaca acgatacgct ctatggatgt accacactgc
      241 agacaagctt gtcctgtgcg ctggtaaaca cgtaatcgtt cctgaaactg accatagccg
      301 ccactagcgt cacgataatc actcatggta gtcccgcgac atttgattgc ttcagttaac
      361 acttcacaaa cagcttggta aagagttttt atttcctctg ttgtgaggga agcggctgca
      421 cgatcaggac ggatcctggc gcagtgcaga gactcgtcgg cataaatatt acccaaaccc
      481 gctacaaact cttggtttaa gagcagggct ttaatgctgc cctttctctt ggcgaactta
      541 cttaccatat actccacgca aaagtcctga cctaaaggtt ccggacccag gcaggctaac
      601 cccttaatct cacacaatcg ctcctgggtc aaccaataaa tagtgccaaa tttccgcaca
      661 tcatgaaaag ctagacaggt accatctgta aaattaaaga ctacatgggt atgcttatct
      721 ctcgccaaag cagtagtaga aaagattaat tttcctgtca tccgcaaatg gatgacgata
      781 acacttagat catcgagata gagatgcaag tattttcccc ggcgtcccgt cgcagcgact
      841 tggtgcccgg gcaattgtgc tgtaaactct gcgaccgagg gggcttttat ggaaccggcg
      901 taatgtacat cgatgctggc aaaaacgcgc cctggcagta cctgttccag cccctgacgt
      961 attgtttcca cttccggcaa ttccggcatt ttgtcactcc atttatcaga ttttttgcat
     1021 ttcagcccaa ttgtgaccaa ctttcacctc aacgagcaga gggacgctga gctgaatggc
     1081 ctcttccatc tctctacgta ctatttttac tacttcaggt aaaatagctt catcaacttc
     1141 caacactaaa tcatcatgaa cctgtagtat cattcgtgct ttatctcgcc aaggctgcag
     1201 ctgccgctct acattaagca tcgctagttt aataatatcc gctgccgagc cttgaatggg
     1261 ggtattcata gctgtacgtt ctgcaaaact gcgtttattg aagttacgat gattaatgtc
     1321 tggcaaatag cggcgtcgat ttaaaatagt ggtaacatag ccgttttgtt tagcctctgc
     1381 cacgatgcgc tgcatgtaat catggacgcc cggcagacgt tcaaagtaac gttcgatata
     1441 cgatttggct tcctgcctgg taattcccaa ctggcgggaa agaccataat cactgatgcc
     1501 ataaattatg ccaaagttaa cagctttagc ccgttcacgt aaagctttgc ttacttcctc
     1561 gggcggcaca tcaaagacct cggaggctgt acgcagatgg atgtcttctc ctgccttaaa
     1621 ggagctgatc agcacttgat cacctgaaat atgagccatc acccgcaatt cgatttgcga
     1681 ataatcagca gataaaagga ctttaccttc agttccgggg acaaaggcac gacgaatacg
     1741 acgcccctcc tcaagcctga tcggaatgtt ttgcagatta ggctctgcac tgcttaaacg
     1801 ccccgtagca gttactgttt gcttaaaagt ggtatgaata cgcccggttt gcggatttat
     1861 gagtttagcc atacccgcta gataagtgcc gagtaatttt attaaagtac ggtactgtaa
     1921 tattttttcc acaatttcat ggtgtggtgc taactcttcc aaaacctggg cattagtaga
     1981 ataaccagtt tttgtttttt tcaaagctgg caaccctaat ttttcaaaaa ggatcaccgc
     2041 caattgtttt ggcgaattta aattgaattc ttctccggcc aactcaaaga tatcttgctg
     2101 cagcacagcg atccgttgac tgaactcatc acgcaaggct gctaaagtcg cctgatctac
     2161 ggctacccct tgccgctcca tacgtgctaa cacggcagct aacggtaatt caagttgatg
     2221 ataaagggcg gccagatcaa attcttgcag ttttgctaat aagacaggat ataaatcaaa
     2281 aagcgtacgc acagcagcac aaagataatt actttcgcag gttgtatccg tctctttttt
     2341 gccgggagcc tgcggcaggt ggcgctggag ataatgctgc gctaattttt gtggtggata
     2401 accattttcc agcgggtcga gcagatatgc agctaaagca gcatcaaaca gtggtgcagt
     2461 gggctctttc cctgttaact gccaacaaat attaatccag gctttgctat cgtaagttaa
     2521 agtagtaaaa gaactgtcga gcagcatctc aagcactact gctagatcac gctgctcagt
     2581 taacggtaaa aatatggttt cgacacttgt tgctacggca aggcccttca gcgtctcctg
     2641 ccaggcagca tcaaggggca gcggcgtaat agccacagca ctttcttctg ccagggcagg
     2701 aaaaaattct ctccactcat ttaatgtcac ttgacgataa gcttccgtag taatttgttc
     2761 cgccgcccga ctttcactgt ctggtaattt atccagtaaa gaattaaact ccagttgagt
     2821 aaaaattcgg cgtaacccct cgagctgcgg cgcctgacgc tgatagtgtg caggcacaaa
     2881 atttagggga acatctgtca caattgtcgc taaatactta cttaaatagg cctgttcgcg
     2941 gttatcctca agtctggacc gcagttttcc ttttatctct gccagatgct cataaacacc
     3001 agccattgta ccaaattgcg caagcagatg ctgtgcggtt ttttcaccga cgcccggcac
     3061 acccggaata ttgtctgagg catccccctt cagcccttta taatcgataa attgctgagc
     3121 cgttaaacca tatttgtttt ttaaagtctc ttcattatag cgttctacct gcgtaatccc
     3181 gcggcgcgtg aaaattactt ctgccggaat atccactaat tgaaagatat cggcatcgcc
     3241 actaacaatc acaggcgtca attgatgctt tttgcccagc actgctagtg taccaatcaa
     3301 atcatcagct tcgaagcccg ccatttcctc caccggaatt tgtagtgctg ctaaaacctc
     3361 gcgcacataa gaaaattgtt ctgacaactc ttggggtgtt ttttcccgcc gacctttata
     3421 tgcactatac ttttcatggc gaaatgtttt tttgcttttg tcaaaggcta cgaccacata
     3481 gtctggtgcc tcatcctcta gaacttttag cagcattgtc gtaaaaccgt aaacagcgtt
     3541 agtatataac ccttgctttg tttgcaaaag cggcagtgca aagaatgcac ggtaaaccaa
     3601 gctgctacca tcaataatta aaaatttctg cttgtccttc acggcagcaa cccccttctt
     3661 ttttttctgg ccatcattag taagataata ataaccgtgc tcagtaccag attgactacc
     3721 aaacgcggcg aaaatctagg ctgtgctggg cgtttagttt gtctttgtgc ggcgacaacc
     3781 gctgactcat ctgctttttt tattacctgt tcttcacttt cagcccctga aaactgctcg
     3841 gctggcggag aggagttatc aaacatgaca gcactaccat cataaccgcg tatttcaggt
     3901 gtcccatcag ctgcccatgg cagcaggtta ccggcaatta ataggacaaa gacggcagcc
     3961 gcggccataa tttgtactaa accaggggct aaatgagttc ggtttgcttg tttttcttgc
     4021 tttaaggtgc taagcacagc actgcgtaag tttggtggcg gcgtgagctt ctctgcctgc
     4081 tgcacccatt gtactgcact ttttgtttct gttgttagct ttctacaaag agcgcaagtt
     4141 tgtagatgtc gttcgtaagc atttttccgt tcagccggta gcagctcatc tagatattct
     4201 gccatgctct ctattacttc ttcacaagtc attttttagc cccctttctt tgttggatgt
     4261 gctcaggctg aatttgttcc tctttttgca gataaagttc acgcaaactt gcgcgcgccc
     4321 tatttaaacg tgatttaagc gtacccattt ttacctgtaa aatagccgct atttcctcat
     4381 agctgaaacc atcgaggtcc cgcagtgcca agactgcgcg ttgtgcaggc ggcagttgtg
     4441 ttagtaaagt ttgaatttca aactgcagtt ctcgcctgat agttgcagtt aggggatctg
     4501 gagcatcatc aggtatttgc cgctgcagtt ctccgtcctt tgtggaaatt ggttgatcta
     4561 gcgagagcat gggctgatat tttttgcgct tacgctgctc atctaaacag gtgttggccg
     4621 caatacgata aagccacgtg gaaaactggg cttggccttt aaataacggc agcgcccgaa
     4681 aggcctttaa aaacgcttct tgtgctaaat cagcagtatc ttccggattg ccagacatgc
     4741 ggtaacataa ggcatatact tttttttcat aacgctgcat caattgatca aaggctaaca
     4801 aatcgccagc ctgacaacgt ctcacaagta ttttttccct ctctggcaat ctgctcacct
     4861 tgcccttcaa tgatcttgtc ccaccacaaa ttatagcacc ttggcactcc ttggagcaag
     4921 ctgctcaccc ttacgatcac acagccagaa taaataaagc cagaattact gatcgtgtaa
     4981 ttctggcttt agcttgtcat acggactaaa ctactttagt ttccgtttgt aacattttaa
     5041 tagcttgtac cacattctgc tcaccaaaaa cggctgtgcc agcaacgagt acggtggcac
     5101 ctgctgcttg gaccagcgat gctgtagttt gattaatgcc accgtctacc tggatttcaa
     5161 ttttacgctg cccaatcata tttttcagct tttctatttt tggtaacatc gcggggataa
     5221 atttttggcc gccaaaaccg ggattaacac tcataattaa tactaaatcc agatcatcaa
     5281 gcacatgttc cagcacagag agtggtgttg ccggatttaa agccacaccg gctttgaccc
     5341 caagcttacg taccatctgc agcacgcggt gcaggtgcac acatgcttct gctgacacag
     5401 ttagtaaatc tgccccggca gcaacaaaag cttctaaaaa tctatctggt tgttcgatca
     5461 tcaaatgaac gtcaaaaaat aattgactag tgtggcgcag tgactttact aactgcggcc
     5521 ccatcgtcag gttagggaca aaatgaccat ccatgacatc aaaatgcagc caatctgctc
     5581 ctgctgtttc tactttttta acttcttccg ctaaacgaga gaaatctgca gccagcaatg
     5641 aaggtgcgat ctttaccata gttagtaact cctctcctgc tgctttattt catgtaaaaa
     5701 gaataagtag ttatcataac ggctgtgggt tagtttgcct gcacctacag cttctcgcac
     5761 agcacaggca ggctcccctt gatgagtaca actagtaaag cgacaatcaa cgatgtattg
     5821 aaaaaactca ggaaaataga aagctaaatt attactgctg attccaatca attctagttg
     5881 actaaaccct ggcgaatcgg cgatcagccc gccgctgtca agcgagagta actcaacatg
     5941 acgcgttgta tggcgtcctt gtttactttt ttccgaaacc gcagctgttc ttaactctag
     6001 gccgggctgc aaacggttca gcaaagaaga cttgccaacc ccggagggtc ccgccaagac
     6061 agatactcgt tcgtttagtt cttggcgtaa ttggtcaata ccttgattgt atttggcact
     6121 agtaaaaagc accgtataac cagcgaggct gctataaaga tggtggaaat cagccagatc
     6181 atcaaggcgc aaatcctctt tattcacaca aataactgac cttaactgtt gggcttccgc
     6241 taaaaccagc aggcgatcaa gcagctgcag cgaaaggggt ggattttgcg gtgcacagac
     6301 aattatcacc tgatccacat ttgatacctg ggggcgcaac aaatgtgtcc ggcgcggctc
     6361 gatttcttcc agcacaccct cctggtcaga aaggcgtgtg aaacgaacat aatcgcctac
     6421 ataaataccg gtatttgttt tttttaaacg gccgcgggca cggcagcgat attcttcacc
     6481 atctgctgtt tttacatcgt aaaagccgcc cacagctcta attatcatac cccgcagagt
     6541 caatcaccct ttctttttag gggaaacttt ttgtatcaac ctgatcattc cattttactt
     6601 ccactgtacc actgccaaac ccgataatgg taatgggctg accatcacta gtgtaagttt
     6661 ctgttctgtc ccctgtttca tcgtgaataa ctactgtaat agtttcacct gccgggatat
     6721 cttctgtgtt aatttcaatg gggtattgtt ccagctcagg tgtgggtagt tctttgggac
     6781 ccttactgag atataaatct acaaagtcac cagcctgtac catttcatta ggttctggaa
     6841 attgactata aataagcccg gcgcctatat cttcattgtt ttcttcagca acatggcgta
     6901 tgtttaaatt aaacagttct aaccaggtag ttgcctcttc cagtgataac ccccgcagat
     6961 cttttagttt aaagggttga ccccccttac taatattcaa agtcacggta gaacctttcg
     7021 caattcgatt acccgcaccc ggatcctgcg agactacagt atttggtgct tccacgctgt
     7081 gtacttcatt tatttccact acaaaattct cttctaaaag taaagcagct tcccgacgca
     7141 tttttcccac gacactatca acttgcacga gctgcggccc tgtacttaat gttaaagtaa
     7201 tctttcgacc ttctttgact tttttgccag ccgctgggtc ttgacgaatt actgctcctg
     7261 ccggcacagt atcactggga atttggtcag ctaaagctgc tgttaaccct gcctcttcta
     7321 aaattatagt ggccttttgt agtgtttctc cttctactgt cggcacctcc acctcaggca
     7381 catagaagac ctgcagcaga cgtacgacac caaaggcaaa gagcgctaac aataatactg
     7441 cgccagcaat agctgctgtg cgtgcccttg gtctctttgc tgccgtttta tgttggcctt
     7501 tgctcatacc cttgccacct gtgataggca ccggctccat cgtggagcgt cgcccttctc
     7561 ggccttgcag ccaagcagtg agttcagagg ccatggcctg caccgaagga aaacgatctt
     7621 ctggttcttt ctccaccgcc cgactaataa tctgattaat cgaatctggt atttcaggat
     7681 ttaaggcttt ggctttgggg aagggctctt gtaaatgttt tagcgcaata gaaacaggtg
     7741 actgcccctg ataaggaacc tgaccagtca gcatctcaaa caagacaata cctaaagaat
     7801 agatgtccga tttttcgtct gtcaggccgc cccgagcttg ctctggtgaa aaatagtgta
     7861 ctgaacctaa aattgagcca ttattgtaag ttatagtgga agcagtaacg gcttgagcaa
     7921 taccaaaatc agtgactttg atgcgcccgt cttcactgat taaaatattg tgcggcttca
     7981 catcacgatg aataatgccg tgtttatggg cgtgttctaa agcctgcgcc atatgtaaag
     8041 cgtactccac ggccatgcgt acaggcaggc gttccagctc agtaattagc tctttaagag
     8101 acctgccatg aatatattcc atcacaatat aatgaatacc atcttcataa cccacatcat
     8161 aaatactaac aatattagga tgtgaaagac ttgctgccgc ttgtgcttcc cgacgaaaac
     8221 ggcggacaaa gtcctggtca ctaacatatt cgggacgtaa tactttaatg gtgacatggc
     8281 gattaaggag gttatccatc cccttataaa caaaggctgt acctccatca cctattttct
     8341 ctacaatttg atagcgattc cccagtatct tcccaatcat ctttttcacc tctagtcgtt
     8401 gaccaagagc taaatattat agattaaaac agctgtaata ttatcggtgc cgcccttctc
     8461 attggccaga gctactagct cttgtatctt ttccagcggg gcattatctt gtgtcataat
     8521 gtgtttaatc tcagcattgc tcaccaggcc atataagcca tcgctgcaga gcaacagtcc
     8581 gtcattttgc agcaatgtta agggcagcag atcaaaaaca gcaacctcat ctgtgcccaa
     8641 agctcgcgtt aatatgttgc gctgcggatg accatcttgc tcctctgcac taatctgccc
     8701 tgttctaact agttgtgaaa ccagggtatg atcatctgtc aggggtaaaa gatcatcttc
     8761 acgcaataaa taagcacgac tgtcaccaat atgagcaagc caagattggc cggcaataac
     8821 catcaacatt gttagtgtcg tgcccatacc tgcataatct ggattagtag tgcctgcgtt
     8881 caacacttct gcattagctt gagccagcat ctcttcgagc agtgggcgaa ctgtttgccg
     8941 attgtcagct gccatgagaa attcctgtct acggtccgcg gaaaaattat ggacaacttc
     9001 caaagccata ctgctggcaa cttcgccggc agcatgacca cccatcccat ctgctaccgc
     9061 atagatagca atagcttcat catcatctat tagataacga tcttcattat tagaccgaac
     9121 ctttccccga tggctaattc ctaccgcttg caatcgtttc acccctcgtt ctttggctcc
     9181 ccacagtagt tgttacggag ttgcccacag gcagcagcaa tatcggcgcc cattttacgc
     9241 cggatagtta cattgatacc tttagcagat aaaatagctt taaaagtatg tattttttcc
     9301 ggtgggcttg cttgccaagg caattcttta acagtattaa agggaataag attgacatga
     9361 cacagccgtc ccgcaagtaa gtgggcacat tgtttagcat gctgtttttc gtcatttaca
     9421 ccctcaatta atatatactc ataggtaaca cgtcggcctg ttttttgggc atactcatca
     9481 caggccggca gtaatacttc caggggatat tttttgttaa tgggcattag ccgattgcgc
     9541 acctcattat tgggagcatg taaagagact gctaaattta aagaccagcc caaagtggct
     9601 aattgcaata ttttaggagc taggcccgaa gtagaaagag tgagatgacg cagactcatg
     9661 cctaaacgct gtggatcctg aactgcttct agaaatggta ttgttgccgc ccaattatct
     9721 agaggttcac cagaacccat cagcaccatc cccttaagtt cacgaccctg ggcacgcaaa
     9781 gtgcgtcgta catacaacac ttgggccata atttcaccca tcttcaaatt gcgtacaaaa
     9841 ccggggagac cagaggcaca aaagcggcac cccatccgac agccgacctg tgttgaaata
     9901 cacgcgctgt aaccaatctt atacggcaga acaacagtct caacagtctg tccatcaggc
     9961 agcacgaata gtattttttc ggtaccgtct attgaacttt cctgtgctgc cacagcaatt
    10021 gtaccaatca cagccctctt ccccaactgc tctcgcaagg caaggggtaa attactcatg
    10081 gccataaagg aagcagcatc cttttgccac agccattcca tgatttgttt tgtcctaaag
    10141 gcaggttctc caagatcacg caaaaaccca gttagttcag ttggtgtcat gcttaaaagg
    10201 tctatttttt gcaactttac acgtcctaaa caaagagtaa gctcccacgt ttaaattcca
    10261 aattgagcca gcgtctgtta ttgctagcgc aagtatatca aaagagcttt taaaaagcaa
    10321 cgtttctcaa ggcatagttc agcggctggc gccagcggct ggcgccagcc tctggcttat
    10381 gctttcttct gcagtttagc aataaaaaaa ccgtcgacct cttgcaggtg tgggtagagc
    10441 tgaataccgt aagtacctat ctgctcgtgc caactaagag gaagataagg ggtaagatct
    10501 gccgcagcaa agttctgctg acggtgcaaa aaggccttaa tgatatcttc tgtttcttcc
    10561 ggttcattag tgcaggtact gtaaactaaa gtaccgcctg gctttaaaac ttgtgccgcg
    10621 gcgtccaata tttctcgctg cacagcggcc agagcagcga tatccttttc ctgacgccgc
    10681 cacttaatat cgggtttacg ccggataact cccaagccag agcaaggtac atccaggagt
    10741 acatgatcaa acatgccaaa atgttgagga agtattgacc gcacatctaa actggctgtc
    10801 tggataatct tcacaccaag tcgactggcg ttggcctgaa ctaacgctaa acgatgctca
    10861 tacaaatcta gtgctactac ttcacccgca ttttccatca actgtgccaa gtgagttgtt
    10921 ttaccacctg gagcactgca cccatctaat atttttgccc ctgtaggcgg gttcagaaca
    10981 tgggatgtta acatcgcact ttctccctgt atctgaaaga gaccttcatg gtaagcagct
    11041 gcggcagtaa gatgaccgcc gccactaacc aagatgccgt caggaaccac cttactggcg
    11101 attgcttgga tgttttcttc tcccagccgc atcagaagct ggtcacggct tgtccgtagc
    11161 gtattaacac gtgccgacgg cggcggcgtt tgattgttag ctgccagcag tttttctgca
    11221 gcttctctgc cgaaacgctt gagccagcgc tttaccagcc attcagggtg cgcatagcgg
    11281 atagttagat aagcaagtgg ttcgagttcg gggttgggcc acaccagtgc atccttttta
    11341 cgtaagaaac cacgcaggac gccattgaca aaaggggcta gggcgcgttt cttactcttt
    11401 ttaatcagtt ccactgtgga atgaacaaca gcaggtgccg gcagacgatc taaataaaat
    11461 aattgataaa agccgatacg taaagcttgt aaaacaggtc ggtccagttt agtaactggc
    11521 cgacctgcta tctggctgat taaccaatcg agggtcaatc gataagaagt gacaccatat
    11581 gttatttccg tagctagtcg cttatcgcgt acatccatag tatgagctgc gaacacagct
    11641 gctaaagcca gattagcaaa agcgtcttct tcctcaattt tgttaagtgc cagcaaactt
    11701 gcttccctgg catttaatgt ttttttcgcc gtcatcctat cttctccgat ttcttaaaat
    11761 cagtaaacgc agcagttggg tgatagagac agcaacagca gccacataag taagggcagc
    11821 tgcacgcaat acttcccgcg tcggaccaat ttctgcgcta gtcaaaaacc ctctattgtc
    11881 taataaagca atagctctgc tagaggcgtt aagctcaaca ggtaaagtta cgagctgaaa
    11941 taacaccgca aaaccaaaga gcataatgcc gatttccatc agtaggccgc cacggaacaa
    12001 aaatcccagg aaaaataaag gaaaagccaa gcgtgagccc agattggcca cggggaaaat
    12061 attgttgcga aaggctaacg gcaagtaacc ctccgcatgc tgcatggcgt gccctgtttc
    12121 atgcgcagcg atgcccaaag ctgccagcga agagctttga tagacagcgg gagaaaggcg
    12181 taccacctgt tggcgcggat catagtgatc agtaagttgt ccttgcccta gctcaacctg
    12241 gacatttgtc aagcccgctt cgttcaataa ttggcgggag acttgcgccc cagttaaacc
    12301 ggaacgcgcc tgcagtcgtg tgttacgttg aaaagctgat tgcacctgac tttgtgccca
    12361 gatggcaaaa aacaaggcag gcagcacgat caaccaggta aaatcaggca gaaacataaa
    12421 catctctatc gcctccttga gtattgtcaa gctttaagtt gtgaaaaatt gaatcgcctt
    12481 ttccacagcc aacaaattaa cctgtgtatt aaaacaaggg ccgtggggtc gttcattagt
    12541 tacacctaac actggcagcg gaatgcaatc taaaatgcca ctagcaagat ccctctcaca
    12601 agctactgct acaatagctt tagggcgtaa tttcttcact gcatctctag ccagggtgcc
    12661 ccctgtagcg atctggacat gacagccata gctatcacag aggttcaaaa tacctccaat
    12721 cggacagcgg ccgcagcgag cacaattatc agttgtaatg gtgatacgct tgtcacagtc
    12781 tgtcatctgc aggcagtgcg gtaagagtac cagcagccgc tcgggtgcaa gctgtccgcg
    12841 ttttgctttc actaattgat tgttgacttc gacaaacgaa cattgaattc tttcttgagc
    12901 tatcttaaac attttaccta tccaaataac aacaggatac agtagcgaaa cagtaaaatt
    12961 aattggttta tgcagccaaa caagatcatg atccgagact aatgacagga taatgcccga
    13021 gaaaccaagg gcaataaatg ctaggcctcc caaaattata atcacagcaa aaatcatgag
    13081 atagcgaaaa aaaccggcat catgctgaaa aaaggataac cacagataac ctaaaacacc
    13141 agcaaataac accatagcgc cagccaacag gcctagatag agcctttttc taatcatcgc
    13201 cctttccctt ccttcctttc catctctagc tcagtatagt accaagtgca agatggtgac
    13261 cacggagaaa atcagcagct aacattgttt ttttccccgg tggctggagt ttagtgatca
    13321 gataagcacc atcaccagta gctactagta aaccctcttg gtcaactgct aagactgcac
    13381 cacaaggatt cccgtgcttg gcaaccggcc taccttccca tattttcagg cgttgatcat
    13441 ttaactcagt atacgcccca ggccacggat tcataccgcg cacctgatta tgaataacct
    13501 gactactctg gcaccaatcg atctgttcat cttctcttgt taaaggtggc gcataagtag
    13561 caagttgatt gtcttgtatt ctacgctggg cctgaccatt attaattgcg ataattgctt
    13621 ccacgactaa tttcgcccct aaatctgcca atttatgatg taaagaacct gtcgtatccg
    13681 ctgccgtaat gggtagggcc tgctggagaa tcatatcccc ggtatccata ccttgatcca
    13741 tatgcatgat cgtaatgccg ctctcttgat caccgttcat gatcgcccgg tgaataggtg
    13801 ccgaaccgcg ataatacggt aacaatgaag cgtgaacatt tagcgcacca agacgcggca
    13861 gagctaatag tgccagggga agaattttcc cataggcaca ggtcaccaga tagtccggct
    13921 gcagtcctgc caaccaagca gcaaaatcag cttgccgcag atcctcaggc tgtacgacag
    13981 gcatctctaa agctaaagca gcttttttca cggcaggtga ctgcattttt ctaccgcggc
    14041 ctgcgcgtcg atctggctgc gtgacgactg ccaggatgtg aaagctgccc gccaatgcct
    14101 gcagtgaggg cacagcaaat tctggtgttc ccataaaaac aagggtcgtt ttagccattc
    14161 tttcctcccg tgaaactctt actcttcttg ttcaataaaa cgagtagctc gatccacaaa
    14221 caaaactcct tctagatgat caaactcatg ctgacaagct cgggctagta aaccctcagc
    14281 ctcaatggtt attttagtgc ctgtttcatc tagcgcctcc acagtaactt gcgcggcgcg
    14341 ctctacttct ccggcgatcc ctgggaaact caggcagcct tcaatagctg tttctttacc
    14401 actacggtct ataattttag gattaatgag ttttaataac cctctgtcgt cctgtacatc
    14461 aatcaccacc agacgcttac taatgccaac ttgaggagca gcaagcccca cgccttgagc
    14521 atcatacatc gtctctgcca tttcatttaa taatttagta attgcaggtg ttattttacg
    14581 gacttcccgc gctttttggc gcagagccgg atcaccgttt ttgcgtattt gacgaagtgc
    14641 catcatcatc gtcctctcta tagtgcttgc ttctattata ataaactttg tggctctacg
    14701 tccagtgcta gccgcacgct tccagcaagc ggcgtttcca aaaaagcagc tgcagctttt
    14761 ttggcagctg ctaacaacgg ttctaaagaa tcgccacgca ctaccagctg ccagcggaag
    14821 cgattacgta ccttctcaat ggggcaaggt gaaggaccaa gcagctcaat atcttccgca
    14881 aatgttgaaa ctaaaaatga acagctatcc accacggctc cctcttgctg accactaacc
    14941 agcaaacgaa gcaggaaacc atagggcggg tagcctaagg cagctctagc ttggctctct
    15001 tcagcataaa atgattcata atcatgtgtt ttcgccgcca caatcgcata atgttctggc
    15061 gtatatgtct gcaccactac tttcccgcca gcagcaccac gtccagcacg accagcaact
    15121 tgtgtcagca gctgaaaagt acgttcgcca gctctaaaat caggcaaatt aatggcagta
    15181 tctgctgtaa ttacaccaac taaagtaacg ttgggaaagt ctaatccttt ggcgatcatt
    15241 tgcgtgccaa ctaaaacatc cgctttttgc tgcttaaagg cagttaagat tttttgatga
    15301 tcacctttgt gcgtggttgt atcggcatca agacgaagca ctcggtaatc tggaaactgt
    15361 ttaagcaatt cagcttctac tttttgtgta cctgtgccaa aatgtcgaat atagcggccg
    15421 ctgcaaacag gacaggtgtt agggtagggt tcatgatgat cacagtaatg acaacgcaat
    15481 gttgtggcag cggcatgata tttaagggca acattgcacg ctggacaccg catcacatgc
    15541 ccacattctc gacagagcac aaaagtggca taaccacggc gattaagaaa aagaataatt
    15601 tgtttccctg cggccacagt ctcttgtaag gcagttatta acggacggct aaagatgctt
    15661 ttatgtccat ccttcaattc ctcccgcata tctaccactt ctacgggggg caaaggtctt
    15721 tgggcaatcc gttccggcaa ccgcagcagc tgatagttac ctccttcagc tgcatgatat
    15781 gactccagtg aaggggtcgc actgcctaag ataaccttgg cgccatgcca ttgtgctcgc
    15841 cacaaggcaa gttctctcgc atgataacgc ggcgactctt cttgtttata ggtagtttca
    15901 tgttcttcat ccagaataat taaacctaat ttttttacgg gggcaaaaac tgcagaacgg
    15961 gcacctatga tgaccaatgc ttcaccacgt gctatccggc accattcatc atagcgttca
    16021 ccgataccca gccgactatg taaaaccgcc actctgtcac caaaacgact gacaaatcgt
    16081 tctgtcattt gcggcgtcag agctatttcc ggcactaaaa caatgcttgc cagcccggca
    16141 gataagcgct gggcaatggc ccgtaaatac acttctgtct tcccactgcc agttacacca
    16201 aacaataaaa caggttttaa ctcctcggac tgattaatat aagcaagggc ttcgcgttgt
    16261 gcgttggtta gggaaagtgc cgtttgtgcc tctacagcag tatctgctaa aggatcacga
    16321 cgtacaattt gttccttgat ttccagccag cccttctttt ctaaagagcg tactgtggcg
    16381 gcaccaacgc ccgcatctgc taattgtgtc agcgcaagtg ctccctgccg tgagagcagc
    16441 tgcaaggcta actgctgctt ctttcctgta gctttggctg cctgacctgc tgccagtaac
    16501 actgcggtct tggtcttttt agctcgtaag cctttggtag tacgaccgct ggacggaacc
    16561 atggcttcta aaaactttat ccgttggcaa agataacgtt gggcacccca tgtagccaac
    16621 gccaccaact ctgcagtgag cagaggttct tcatctagtg ctctaattat ttctttaatt
    16681 ttgcctttac tctcccctgg cagctcccgg tgcagccgca cacaatatcc taccagtttg
    16741 cggttgccaa agggtaccag aacacggctg ccgacaacaa gtttaatctt tgctggcaca
    16801 agataggtaa aggggtgttc tagcggtaaa ttatgaatgc tgacaataat atcggcaaat
    16861 atctccacaa tagcctccaa ttaataaagt tagattgaat acttccacat aaagtgtcac
    16921 ttaccttgtt agccaggaaa ttatcccagg ctgcgcaggg ccttctatga agggcataag
    16981 gtcaagaggc gaatgtatct cgatattcct tgcaaatact tgacaggtgg cagtataatg
    17041 tacaaggagg tataaagaat gacaaaaacc ttttttctac tgatccggga aaaccgctca
    17101 tggattattc tggcaatttt atttttcgtc atgggtcttg tttctgccta ctcagcccta
    17161 ggccaagaac cggccttttt acagttatta gaagaatccc taggtgccct aagtgagttg
    17221 ggggaagaaa tattcagcgg ttccccgctg acgggggtct tcgtgctctt cggcaacaac
    17281 ttgattgcca ccatgcaaat tattttttta ggcatctttt tgggcttgcc atccctctcc
    17341 gcggcgattg ccaatggtgc cgttttaggc tttctctctt ttcaacttgt tcaggaaggg
    17401 attgcaccgc tgcctttttt attatctggc gtcttaccac acggcatctt cgagctgccc
    17461 gctttctttc tttgcgtcgc gttgggttta aaactagggt atcatgttat ttttcccctg
    17521 cctgaactca cacgtcgcca aaccttccaa aaaatattta aagaaattgg cattgcctta
    17581 ccttatattt taggcttatt agctattgcc gccgtaattg aagtatttat tacgccactt
    17641 cttctcggct ccttgatggg cttgatctaa ctaaatagtt tttaaatact tgagagccct
    17701 gtttttttgt aacagggctt ttctatgctt tcccatggct aacaaagggg gtttttaatt
    17761 aagaagcttc tgctgttgct aataattcac taattgccgc aagcaggatg tcggcaactt
    17821 cgcgtttggc cagcttgcct aattgctgct cacgcccgtc acgatagtaa agacggacga
    17881 tgttggtgtc aacggcaaaa cctgcccctt cctgcaggat gtcattagcc acgattagtt
    17941 ctagattttt acgttctagt ttagccaagg cgttctcacg cagattttct gtttctgcag
    18001 caaagcccac taaaatttgc tgctttttta tttgccctaa agccgccaag atatcaggat
    18061 tacgagtcaa gttaatcact aattctcctg attttttaat tttttgctca ctgctggtac
    18121 ttggccggta atctgccaca gcagctgcgc tgatgaccac atccgtacta gcaaaacgag
    18181 ccagtaccgc gtcgtacatt tcctgcgccg tggttacgga cactaaatcc acaccgtatg
    18241 gcggagggag catgaccgga cctgaaacaa gtgtgacggc ggcccccaat tcggctgcag
    18301 cttgagcaag ggcatatccc attttgccgc tggagtggtt tgtcaaaaag cgaacaggat
    18361 ctaaggcctc ttgcgtaggt ccagcggtaa taagcagacg gcgaccttgc caaactttct
    18421 tctgccgtaa aagacgacag gccacggcaa aaatatcttg tggagacgac atgcggccgc
    18481 gaccactatc gccgcaagcc aactcacctt gatcaggtcc aacaagagca taaccgtagc
    18541 tctcaagctt cttaaggtta gctaaatgaa tagggttttc atacatatgt gtgttcatag
    18601 ccggcgccag taaaattggg catttagcgg ccatcaccgt tgtcgttaaa aggttatcgg
    18661 caataccatt ggcaatttta ccaatagtat tagcagtagc tggggctaag acaattaaat
    18721 cagcttcttg cgccaagcca acatgctcta cagaaaagcg gtcgctgcca ataaaaagtt
    18781 ggtcatagac ccggttgccg gtgagagtct gaaaggtaag tggggtgaca aactccttag
    18841 ctgcttccgt catcaccaca cgaacttctg cgccagcctt aataaaaaga cggcaaagtt
    18901 cagctgcttt atagacggca atgccgccgg taactcctac gacaatgctt tgaccagata
    18961 gcattacact tccccctatt ttttgatgcg ctcgtaacct atcttatcag cagctatttc
    19021 ctccaaggca atggaaactt gttttgttgc gtttgactta atgagtgctg ggtgattttc
    19081 attaagcatc cgcgctcttt tagcagcagc aatcaccaat gtatatttac tatcgacttt
    19141 agtcattaag gtatcaatgg acggataaat caacctttaa cctcctcagc aagtatctga
    19201 attaaactct ggttgcgaac aacacgcagc tgttctgcct gaataatagc atgtatacgc
    19261 tcactagcag aggtgatctg atcatttacc acaacatagt tgtatgcact ggcccgttta
    19321 atctcctgtg aagcagcggc aagccttttt tgtaaacttt ccgtactttc tgtaccgcga
    19381 ttaataatgc gcttccgcaa ttcagttccc gacggaggta ataaaaaaat aaatacgcca
    19441 tctggatatg atttcatcac tttttgcgca ccttgtggat caatttccaa aattacgtta
    19501 ttgccggcag caagcttttc ttccacagct acccgtggtg tcccgtaata atgatcataa
    19561 acacatgcat attccaagaa atcttgttgc gctattgctg ctttaaattc ctcttccgta
    19621 acaaaacaat agttaacccc gtttttttca ccgcagcgtg gttgacgtgt tgttttagag
    19681 actgaaacca ccagtttcgg gtctttttca agtaatgctt tgcagaccgt accctttccg
    19741 gcaccggatg gtccagataa gacaattagt aaacctggtc taggcacaat attcctccgg
    19801 ttgtattttt agtcttcgtc gtcagcggca ggtgtttctc tggtatgtaa cctgtgcttg
    19861 actgtttcag gctggaccgc ggaaagtact acatgtccgc tatctgtcac aataacggca
    19921 cgcgtgcggc gtccataagt agcatcaatt agcatgcctc tgtctcttgc ctctgcaata
    19981 actcgtttaa tgggtgccga ttctggacta acaatggcaa taatgcggtt ggctgaaacg
    20041 atgttgccaa atccgatatt gatcagctta atttgcaacg ttagaacctc cttttatctg
    20101 gtaacacccg ccatagctta aagtttttgc atttactctg ctaatagtat aacggatatc
    20161 cagtaaaaaa tgcaagagtc aaatataagc gccgttttct cactcattct atattttgca
    20221 cttgctcacg tatcttttcg atttccgttt tcatctctac aactaatttc gccacctcat
    20281 aatcattagc tttagagccg atcgtgttag tctcacgaaa taattcttgg gttaaaaaat
    20341 ctagcttacg cccaatagca cctgtttctt ttagtgttgc ctgaaatgct gtcaggtggc
    20401 tggttaatcg tactagctcc tctgtcacat tactgcggtc ggcaaagatg gcaacctctg
    20461 ttaatacccg tgcagggtca taatcagctt gtcctaggac ttcatttaag cgctcttcaa
    20521 gtttgctgcg gtgttcctct ataattaaag gtgttcttgt ctccaccagc tcggctgcct
    20581 gtgccacttt tgtcaagcgc agcttaaaat cttctaataa acgagcacct tctgcttgcc
    20641 gctgctcgat taagttgttt agtgcctgat cgagtacaaa tgccagcctt tcccacacct
    20701 catccaagtc aacttggggt ttttctacac gcagcacatc aggaaagcct gctaaaagtt
    20761 gtaagcttgc cgactctttg atcgcataga gctcacttac ttcctgcagt gcacgttggt
    20821 atgcccgcac gagcggcctg tcaactgtta catttacggc ggcgtctgtt tcatcgtgcc
    20881 aggaaataaa aatatctact ctgccgcgtc ctagcttttg ctgcacacgc cggcgtatcc
    20941 gctcctccaa ggtttgaatt tcccgcggca tacggataga aatgtctaga taacggtggt
    21001 taactgaacg catttctact ttgatgcgtg taatttcgtc tacactattt tcagcttgac
    21061 cataaccagt catactcccg atcatcgaag tcccttctct ctgttcctta ttgcaaccaa
    21121 tactacaaaa tctattgtat cagaaaaata gcatttggca aatatcgtca agtttaacaa
    21181 tttacttttt cataattaca acacgagggg caaaagcact cttcagcaga gagtaaagag
    21241 cattaattag taccggtaaa aaacaagctg tcaagatgat tatccattgt tcactttgca
    21301 ggggtcgagt aagaaaaatc gctgttaata atggatgata cagcaccgct aagagtaagc
    21361 ccattgatga ggctaccgct gctaccagcc aggtgttgga aaacaaaggg atttgcagca
    21421 tacttctctg ctcactgcga caatcaaaaa cataaaccat ttgcgtgata atcagcgtcg
    21481 taaaggccat cgtcctggcc tcatcaagct cccaaccctg ctgcagcgcc caggcaaaaa
    21541 tgagaattgt tgtcaagcca attaccgtcc ctctgcctac tatcttttgc cataatccgc
    21601 gcgcgaaaat gctttcacgt ggtgaacgcg gcaaccgcgc catgacatcg gaatctactg
    21661 ggtcaacgcc cagcgccatg gctggcaggc catcagttac taaattaatc cagagaattt
    21721 gaatagcttt aagtggtatt ggcagcccca ctagcattgc taccagcatc gttaagattt
    21781 caccagtatt gcaggccaag agaaagcgaa taaacttacg aatgttatca tagatgcctc
    21841 gtccttcttc tacggcactc acaatcgtcg taaaattatc atcagtcaag actaaagccg
    21901 cagcttcttt agttacatcg gtaccactct tacccatggc aatgccgata tcagcctctt
    21961 taacagcagg cgcatcattg acaccatcac ctgtcatggc cacaatatga cctcgccgct
    22021 ttaacgctcg gacaatgcgt aatttatgtt ccggggcaac acgagcataa acataggtat
    22081 tagtaactgc tttttctagt tctttgtcat taagctcatc caattctcgc cccgttaata
    22141 cctgaccctg tagaggcagc aaatgtaatt tgcgggcaat ggccatagct gttgttttat
    22201 gatctccggt aatcattact gttttaatgc cggcagctga gcacttctgg atggcaccaa
    22261 gcacttcagg gcgtggtggg tcaatcatcc ccaggatgcc cacaaaaatt aacttatttt
    22321 ctaattcttc tgaaacggtt tgggtgttag tgagcggtcg atacgctacc gctaggttac
    22381 gtagggccaa ggcagccata gtctcgtttt gctgcatgac ctcctgacgc aggctatggg
    22441 aaaattccac tacccgcccg tgataaaaaa tttggctgca tttatccata attacctcgg
    22501 gtgcaccttt tacatacaga atttgtccta ccgttccact atagagaaca gacatccgtt
    22561 tacgtgcacc atcaaatggt atttcaccaa ggcgctgcat actgcgctcc agatcctccc
    22621 gccataaacc ggcacgtgct cctgccacca caagagctcc ctctgtcggg tcaccttgga
    22681 tttcccagct tgctgttttg cctcgccata atggctgtat ctccatattg cccttcaata
    22741 actgcgcatt attgcagagg acgctagcag ttaaggttaa cattaagcca gcatctttac
    22801 ccggttttat ctggcgtgta ccacttaaga attgtccctg ggggctgtat ccttcaccgg
    22861 ctacgcggta atgcctaccg ccagcccaaa tttcctgcac attcatttta ttctgtgtca
    22921 atgtacctgt tttatcagag cagataattg tggcacagcc caatgtttct actgctggca
    22981 aacgtctgac cacagcgttt tttcgtacca tgcgctgcac accaatagct aaggcgatag
    23041 ttaccaccgc tggtaaacct tcaggaattg cggcaaccgc caaggaaatg ccagccatca
    23101 acatattgta agcaggcaaa ccttgcgcca cgcccatcac cactaccagc gcgcagatta
    23161 atagacagcc tatcaccaga taacgaccta attgttccaa acgtctttgt aaaggcgtgt
    23221 cttcattttc agcatcttgt atcaaatgag ccacgcgtcc catctctgtg gccatccctg
    23281 tagctacgac gacgccgctg gcatgacctc cgaccgccat cgtacccata aataccatat
    23341 tgaacctctc acccaaaacg gttacctctt cagacaaggg gtcagaaatt ttcgtcactg
    23401 gttctgattc accagtcagt gatgcttcat tgaccaataa ttgacgaact tcgcacaagc
    23461 ggatatcggc cggaatgcgg tctccagctt ccagcaagac gatatcacca gggaccagtt
    23521 cttctgccgg aatttcgtat cgcacgccat cccgcagagt tttagccgtc ggggcagtca
    23581 attcccgcag cgcctccagc gattgctcgg cccggtactc ctggataaac cctaaaatag
    23641 cgtttagcag cacaacacct ataattacta cagcatctga atactcacct aaaagagcag
    23701 ataaaagtgt agccccaagc agcaccaaca ccataaaatc ccgaaactgc cacaaaaaaa
    23761 tgacaagtaa gggaactttt ttcttttctg ctaagctatt gtggccattt tctttaagcc
    23821 tttgggcggc ctcgctgctg gataggcctt taagagcatc aacctgaaaa tgttgttcta
    23881 gttcagcagt tggtaactga ctccaaggca gcttgttcat cacatcaccc catggttaga
    23941 gatatttgtc ctaccgccat atatatgggc acagctgcaa aaaaagcact tcccaggaca
    24001 agatttatta gtttctgttg ccttgctccc cttttgactg ctatactaaa tgtgttaaca
    24061 aggaggatac gcaatggcat acgatggctt aacgatggct tgtgtacaac aagaattaaa
    24121 gcactcttta ataggtgcac gcatagaaaa aatatatcag cccagtaacc gagaaatcgt
    24181 tttacatctg cggacgcgtg aggccgcact gatgctgctt tgctctgcag atccacaact
    24241 ggcacgagta catctcacta cggctaaacc agccaatcct gcagttgcac cagctttttg
    24301 tatgctgatg cgcaaacatc tgacaggtgc ccgcctttta gatattgaac aagatcagct
    24361 ggaacgggtc ttaacttttg tttttcgctc ttatgatcag ttcggcgata acactaaaaa
    24421 aagattagtc tgtgaggtta tgggcaaaca ttccaatatc attctaacta tgcctaacga
    24481 acaagaaata ccccaagtac ttggcagtat taaaactgtc gatgagacga tgagccgtct
    24541 ccgggtagtc atgcctggag aaatttactt tccaccacca gctcaagata aactgaatct
    24601 ttttgacttt acagaggaag aactaactgc cgctttactg caagctgaaa ataaagctgc
    24661 cgataacgca cttgtacaag ctgtgggggg actaggaaga gaactagcta gggagattat
    24721 ccgacgcgct gcggccaacg agcctgctca tcctttagca atggttcgac ctctaacaat
    24781 agaattacag cggctggctg ccgcctttaa gaatggtaaa cttaccccct gcctgagaaa
    24841 gctggaagac caaaaacctg aattctcgct gctagctcca acctcagccc aacagatcga
    24901 gttaaccttc tatgctaccg taaatgaagc actcgatgct tattatactg cggtcgtgca
    24961 ggagctgcag acaaatgaag tcaggcagca gttactgcat actattaata agcacttaac
    25021 acgcagtgaa aaaaaacagt atctgcaaaa aaatgaactg caagaaatgg agggcgctga
    25081 caaataccgc atctggggtg aaatgctgac tgccagcctg caccagctgc gcagcggcat
    25141 gaaagaagca catgtcatta actactacgc cgaaaatatt gaaaaactaa agatacccct
    25201 agacccatcc ctctcccccc aggccaatgc ccaacgatac tttaaaagat atcgcaaact
    25261 tagcgatggt aaaaaatatt tacgtcaacg tttaaaggcg acttatagcc agcttaatta
    25321 tcttgaatct ttactagtgg ccgcacaaaa tgcagacttg ccagccctgc tcgagatacg
    25381 tgaagaaatg gaaagtgacg gattaatccg cagcagatca cataaaaaac gtgtaacggc
    25441 accaccttca cagcccttac attttctttc tcctgatggc attgaagttc ttgtcggtaa
    25501 gaataaccgt caaaacgatc aactgacgat gcgtttagct gcgtcagccg atgtctggtt
    25561 acatgttaaa gatgtgccgg gctcacatgt gattattcgt catgctgaac cgccaaccag
    25621 tacacttttg tttgctgccg ctttagcggc tcactacagc aaagctgcgg cctcagctaa
    25681 tgttccagta gactatacac aagtacgaca tgtcaaaaaa ccacgcggcg ctaaacccgg
    25741 catggtcatc tatgatcacc acaatactgt ctatgtaacg ccaagcgcag gacataaaca
    25801 ataaaaaaca taaaagacaa agaaagcgta cctacaaagg cacgctttct ttgtctgctg
    25861 catttatact attcggtaca gcaagtgggt ccctctagcg ggttaataag atagcgaatg
    25921 gcagcatcta agataacctg accatcttct tcagccaagc gtaccatacc aatgatacca
    25981 ttttcttcac ctagccactt taggtactct tctacaaaaa tttgcgctcc cacctcacta
    26041 aagggttttt cgtgatcctt aagcacaact aaatacactt cccggggaat gtgcaaaatt
    26101 cggtgagtaa cttgaaagat ttcacccttt acttcctgct cttcgacttt tgcaaatagc
    26161 gaactctcct gcgccattaa cctcacctcg cttacaactt ataaaccgga tcgttctacc
    26221 tttattcttt cattaactgc acaatattat cttgcgttgt cgcgatacga gaaatctttc
    26281 gttcttcatg ctcctcgatg gcacgccgaa attgtgtttc atacagttct gtatagattc
    26341 cctcttttat gacaagctca ctgtgcaccc cacgttcttt aatcactccc tctttgatca
    26401 caagtatttc atcagcggta aggatcgttg agagccggtg ggcaatcaca atacttgttc
    26461 tgcctttgag cagaggttca atagcatcct gaattaaatt ttcagaaatg gagtcgagtg
    26521 atgatgtcgc ctcgtcaaaa ataataatag gcggatcttt gagaattact ctggcaatgg
    26581 agacacgttg tttttccccg ccagacagtt taaggccgcg gttgccgacg atggtctcat
    26641 acttgtcagg taacgacatg atgaagtcat gcacattagc cttgcgacaa gcttcttcca
    26701 attcctcttg tgtggcatcg gctttggcat acaggagatt atcttggagc gtggcgttaa
    26761 acaggtaggt gtcctgtgta accataccaa tcttgcgacg taaatagttc aaatcaagct
    26821 tgcggatatc agtaccgtca agcagaatag aaccgccctg gacatcgtaa acccgcggga
    26881 tcagattgat aatggtggac tttcctgccc cggacgcacc aactaaagct accgacttgc
    26941 cggctggcac agagaaagtt atgccgcgta aaacagggac atcactactg tatgaaaaga
    27001 caacattatc aaactccagc ttacctttcc aagcagaggg aatgatcgca tcagcagcgt
    27061 tttcaatttc aggttgaata tcaaagtatt cgaagatccg cgagaacaat gctaatgatc
    27121 taatcacatc aacctgcaca ttcatcagcg agtttactgg cctatacatt ctggacagta
    27181 atgtcaccat cacggtgatg tcaccaactg tcaggttgct gtgcgggtta aagataataa
    27241 tcatcccgcc gccaagatag atcagcatcg ggcctacact ggtgaaagta ttgagaacga
    27301 ctctgaacca ccgtccaacc atactttcct taatactaat attcaccatt tcacggttac
    27361 tagcctcgta tttgtcatac tcagtcttct ccttggtgaa cagtttaaca agcatctgcc
    27421 cactaacact gagcgtttca tttagtatct gattgatttg gtcgttatat ttttgcgatt
    27481 ccaaagccag tgaccagcgt gttttgccca cccgtttcgt cggtatcgtc agcaaaggga
    27541 ctaaaactac cgccagcgtt gccagaatcc aatttttctg atacatagct gctagtgcta
    27601 caattaaagt agcaatgttc tgaataatac tggttagcgt attgacgatg acattcctca
    27661 ccccgtcgat atcactggtc atgcgggtga tgatatcacc ttgtttgttt gtcgtaaaaa
    27721 aattgtgcga cattttttgc agatgagtaa acatattgtt gcgcatatca taaataatgt
    27781 gctgcgctac ccaagtgttg atatagctct gcagcacatt aactaaattc gaggcgatcg
    27841 ttaaaacaaa agaagcgata atcagcagca gcagtgtttt tagatttttt cccagtagtc
    27901 cctcgtcgat aatgcgacct gtaagcagcg ttgggtagac accgagcact gcagatatca
    27961 tgatcgttat aataacgacg catagttgtt tccaataagg aagcaaatag ctaaagatgc
    28021 gcttaagcag ttgactggtg acttcaggtt tattttgttt ctcttcctcg gtaagatacc
    28081 tgctgcctcg accaccaatt cgcctactct gcataaaata ctctcctctg cttcacttgc
    28141 ttgcagctat caacccgcag caagcctatt gatctcttat caataacgct catgcctttt
    28201 tttatgacgg gcccccccct ttgaaccttt tatgccgatg atgatgatgg ctcccggttg
    28261 aatgaaaatg ttttgtgccg tagctagaag aagaacctgc agattctggt tgatgaccgt
    28321 cgtcttggaa cttttgctct aatactgcta ctatgcgatc aagataatca tttagtgcca
    28381 cctgttcttc tacactcaaa cttttaaaca agtcctctgc atcggcctgc tgctcattta
    28441 aaactgataa ttcttttcct gcatctgtca ggtggatgat cagaacgcgc tggtcctctt
    28501 cagacggtgt tctggtaatg taaccgttct tttcgagttt ttccagtaat tctcctaaag
    28561 attgcggccg catgtcaagt agatgtgata gctctttctg gctgatctcc ggcttgagtt
    28621 ttagtaaggc taaaatacgt ccctgacctc gatggatatt tcccatgggc ccatgctttt
    28681 tagatctctc gtgatagaat ctaccaagca aacgctgtaa ccggaaaaac ttgtttaaaa
    28741 gatcgctatg ctctttgctc atggtaagcc tccctcctaa agttactaag gtaccttctc
    28801 ttcttattat aaggtagctt agtaatttgt caagtggcta taaccctgtt aaatatgact
    28861 ttagtattgt tattagttaa aacttttgac ataatagtct ttataaaata agcagcacgc
    28921 tagcgtcttg ctggagatta tggagctggt tgcaaatggt gaatgctgac aaaaaattgt
    28981 ccgtaaactg attcttcctc tctttcgatg caatctatac gcacttcctg cagctttaat
    29041 tgcctcttta attgttccac aaagtggtag gttggcaggc gacgttggtg ggaaataagt
    29101 atttggccgc cagctttaag gttatcctta aaaatagtaa gcaggtaggc attaagctgc
    29161 ggatcataaa aaatatccga gcctagcagt aagtcaaaac gctgctgcaa agtaaaagca
    29221 cgccagtccc cgagaaatcc ctctgccttt acaccattta aggcggcatt atgtagggat
    29281 agttctactg ccgcttgatg ataatcagaa aacatgacct gaccaccacg gataccgcag
    29341 atcgtaccag gcaaaccaag tccactgcct aattccaaga cagacttgtc aagtaagttc
    29401 aaattcttgt cgatatagcg cgctaaagcg cgtgcagagg gccagatttc agcccagcaa
    29461 ggcactgcat cagcatccga taaatcagtg attaaagcct cgatatcatc gaccacatca
    29521 agataaacaa ttttattagg cagcttaaat tccgttctga ccaattgaat atccatcatc
    29581 tcacctattc ccactattat tatggaggtg tcattatgtt tcgttggcgt cgcattaaaa
    29641 gtacacgtga agtatggcgc aagggagaaa agttaagacg agcaggcaaa acaaaagcag
    29701 cggaagaact tctgctcaaa gctgcagcgt ccacctcacc attggacaag cattatgcct
    29761 accttaaatt aattcagctt tatcaggagc taaataatgc aggtgataat cgtcagcaag
    29821 atttaatagt gacctgtcaa aaggatattg cactattccc agatttttat gaggcctggc
    29881 tgatagaata ttttaataac gttcctactc cctattttcc ctctttttct gtgttagcag
    29941 aaatttatga gcgtgaaggc aggctgcagg aaacaatcga tctttgtgaa ttggcaattg
    30001 gttacaacct acaagaaact agaggcgagg attttccgga aaagttagaa cgtctttatc
    30061 gtcagcgaga tttgcagcaa gagaaatagc atttgttagt ataatttgct tccaaacagt
    30121 gcaatttagg tgaaaaggag gtgataaata tgatgaccac ctttcactgg atccacatta
    30181 tagtgggtgt ttggttagca tttgtcaact ttatgccaat cttaccctcc gaacttctaa
    30241 tgttgaacaa cgccatcatc ggcattgttg tgacccttta caacgcctat ttcttgtttg
    30301 caaaaaataa tacagatgtc aagcagaaaa gttgagctag gcaccttagg gtgcccagcc
    30361 aattattgtt cctgatcaga ggggtgagta aatgagtgaa tttaaaaatg actcacgaga
    30421 aagtgacttt gcatctgtgg cgattaaata cggtgcttgg ctgctgggtt tgattattgt
    30481 gctttacttt gtttcccgca acgttttccc ctttattcaa agtcttttct aaaaaacagc
    30541 gggataacga taatggccga ttatatccca gcgctgtaag caatcttctt cttttgtcac
    30601 actgttatta tgaataacaa gcggtatgcc ctggcgatta gttctatcac taacaatgcc
    30661 agtatggagt tgaccgtctg cacagcgcca ggatacaata tcgccccact gccatgtttc
    30721 cagttggtct gccactgtca ggggaagctc ctggccaaaa cgagagaaga ataccatttg
    30781 attgcgtacc cgacgatgat caatattagg gtcaggtgca tctaaaccat aatcttgtgg
    30841 gtaaagcgca aaatttgctt gcatatcttg gtgaattaac acctgcaaat cgatatctgc
    30901 attacggaga gcacgaatta ccacatccgt gcaagctccc tgcgtctggg aaatatctcc
    30961 gcccgggtaa gctatgaccg cataagcagc attatagcgg accctttttt ttgcctcggc
    31021 tctcgcaccc agcagaatat tatcagctgt aattcgttgg ttggacggtt ttttctccac
    31081 taaagattct cctgggactt taatccattg taaaacgact attacaacta ttaccagagt
    31141 aataattatt aaactactac tttttctacg cagcatcgta ccactcctta ctaatgggga
    31201 tattccctgt ggagggcaat aatcctgcag gcaagctgcc caattgtata gcgatctaat
    31261 cttttgtcta ccacgcaaaa cagccgctag taatagcggc tgttaacgga aacaacgtct
    31321 ttttatttct acagaattaa cgacgcagcc ttcttgcttt agtttatgaa gcagcctatc
    31381 taattgtttt tgggtcattt ctttataaat accagcttgt tgtccctgca gtgtgcggta
    31441 tacaaccata tactctaagt ggttcactag atccgtctcc ctatcttgtt tgctaattag
    31501 cttggttcgc tactattatg ttacaacaag aaggggatta gtatattaat aatttactat
    31561 aattctttcc tctgtgcgga aatagcatat aaaaacttac atttagggta tttgctgcag
    31621 cttttataag ttccatgttt tccttcacga atcattaatg cactattgca tttaggacat
    31681 atatcagctt tgaccagtct gtttcgttct tgcaatagtt ccctgatatc ataaattcga
    31741 gcttgagtgg taactttttt tgcccgttta ccttcaacca tggagataat agcttgcatt
    31801 tgttctgctt ttaaataaac cttttcgtaa ctctttattg tcggcaaaag gtcttccaca
    31861 tcaatgagat gcgctgctac atcagccttt acctcggtaa agatgttaaa gagaacaatt
    31921 ggtgttatcg gtacccgggc attgctgaat agctctcgca aagctttggt ttgcacctta
    31981 cactgctgta aaggatttgg aaatcttttt ttatactgca aaattttcac ccaaaaatca
    32041 ccgtgctctg ctccctcaat ccgacctatt ttttcgcaca gttttatcac aaagataccg
    32101 tgggtagaga tgacaagatg ctcaattaat aaggttctgc ctggtgtgat ctgtaaaata
    32161 agattatgta gtatataata atcgtgatga tcaagttggg caagcatctg ttttgttttc
    32221 cgcctgcgca gcccgcttac aagtgctggt agtctcagcg ctaagtaaat tactgccaag
    32281 acaagtgata aatataacca caattgcgag caccccttcc aagttttttt aacttacgaa
    32341 ggcaacatca ctaaaggaaa aacatttatt aactcctgcc gtactatcat agtattatag
    32401 acgttaataa tttcgac
//
LOCUS       DASRMS010000222         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_400618, whole genome shotgun sequence.
ACCESSION   DASRMS010000222 DASRMS010000000
VERSION     DASRMS010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_400618"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            533..739
                     /locus_tag="VFC74_10655"
     CDS             533..739
                     /locus_tag="VFC74_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25828.1"
                     /translation="MINHKAHWSAIRSNVFAVYETLAKSREPMAYKDMKSGLDEESFF
                     SAVSFLYVRNKIRPVRGGGWEIVK"
     gene            736..3057
                     /locus_tag="VFC74_10660"
     CDS             736..3057
                     /locus_tag="VFC74_10660"
                     /inference="COORDINATES: protein motif:HMM:NF024547.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HZK25829.1"
                     /translation="MRAVDYEDYLNANFIIFPLHPIVNGKCSCERENCDSPAKHPKAS
                     NWQHTQPYDEDQLEYLEDAEGIFFGNQLLDHYGVLVDGKIVVDVDGRNGGFESAKKLQ
                     HIRDQCEFIVRTGSGSGEHWYFDGKDAGRLAQSLAEYPGIDFKSTGFVVGAHSLHVSG
                     LRYEAIKGSPQSITQAPQELVELLRKPERETFKIEGVALEASELHDAVMRIENGAHDY
                     SRWLAVGMGLHHATNGGAVGEEIWEKWTIKTGRTDTESISAKWHSFGKSRNPVTEGTL
                     LSWAKDGGYIQSVTFESDVEWDEPNAQPEREKQLISDFNPDNLTGLAGDVFKWIDSQC
                     LFPRKNLSLAAALQVIGNAAGMRYRVDSIYKTTLNLMTFAIADSGSGKEAVYQAASSL
                     LDAAGVGLAVHGGIKSEQELVRNAVRNQASLYMIDEFGAMLSKIGSAKKKGNASYLEA
                     VPAEIMKIFTKANSSYLVSGDMREEIADRADKKIASIQKRLDDGRGGEKAEQELEHAK
                     TEREQTNSGIPKPFLSFFGISEPNSFDAAIRMDPDMLVNGFLGRALLFRESEALPMRR
                     SDYNPRQMPITLSSRLSQLYKNGSAGAEFERVQLYGDEETITVSEDARAELDSVYAYW
                     RTRGLELEHEGQGLHTITTRVWEMVIKIAGILSIPSEMGRQPIVTLDNVRQAHAIVRK
                     VTDFKISHCITIIGADSKDDGERVAGLFEGVIGVLESYPQGIGAGVLRNRNKKYGRIN
                     VDKCIEKLIEQKRVKEIEYTDTRNRSQKKYKLI"
BASE COUNT          893 a          650 c          821 g          722 t
ORIGIN      
        1 attatactaa atactatata cttcttcttt tagatggctt tacaggctcc atactactct
       61 tttaatgcat atctacagtg tagctgtctc tagcacgtac ttatctctag gcgttgatta
      121 tctactaatc atctctaatc attaaaaaac cctttaaaat caacagcttg gaaagatatc
      181 gttcctgcta accattggta atcatcttga taatcatctc aaacccttct gtgagtgctc
      241 aattttgctt gtaaatgtaa ttattttgtt tatagttggc gggacataag cacaagtgag
      301 gccgcaatga aaaacccata caaagagcta actttcatga acactgtttt cgataggttg
      361 acaaaggtcg ggtcgggcaa agaggttttt tgctgtgttg atcctgcctc tggagtggag
      421 cagccagttg aatcagttcg atctgcaatc aacagagcat ctactgtttt acgcatgaag
      481 tttgcaaccc gactagtaga tgggcagcta atcataaaaa ggatctcgca acatgattaa
      541 ccataaagcc cactggtcag caataagaag caatgttttt gctgtgtacg aaacgctagc
      601 caagagccgt gaacccatgg cgtacaaaga tatgaaaagc gggttagatg aagaatcttt
      661 tttttctgct gttagctttt tgtatgtgcg caacaagata aggcctgtgc gcggcggtgg
      721 ctgggagata gtcaaatgag ggctgtagat tacgaagact accttaacgc caattttatt
      781 attttccctt tgcatccaat tgttaacgga aagtgtagct gtgagcgtga aaactgcgac
      841 tcgccggcta agcaccctaa agcatcaaac tggcagcaca ctcagccgta cgacgaagac
      901 caactggaat acctcgaaga cgcggaaggc atcttcttcg gcaatcagct actggatcat
      961 tacggtgttt tggtggacgg caaaattgtc gttgatgtcg atgggcgcaa cggcggcttt
     1021 gagtcagcta aaaagctgca gcacattcgc gatcagtgcg agtttatcgt gcgcacgggc
     1081 agcggtagcg gggagcactg gtattttgat ggcaaggatg cagggcggtt agctcagtcg
     1141 ttggctgagt acccaggtat tgattttaaa tcaacagggt ttgtcgtagg cgcgcactcg
     1201 ctacacgtta gcggcttgcg ctatgaagcc attaagggat caccgcaaag catcacgcag
     1261 gcaccacaag agcttgttga gttgctacgc aagcctgagc gagagacgtt taaaattgag
     1321 ggcgtagcgc tagaggctag cgagcttcat gatgcagtta tgcgcataga gaacggcgcg
     1381 cacgattact caaggtggct cgcagtaggg atgggcttgc accatgcgac aaatggcggc
     1441 gcggtagggg aggaaatatg ggagaagtgg acgataaaaa cagggcgcac cgacacagag
     1501 tcaattagcg caaaatggca cagcttcggg aaaagccgca acccagtaac cgagggaacc
     1561 ctgctctcgt gggcaaaaga tggcggctac attcaaagcg ttactttcga gtctgacgta
     1621 gagtgggacg agcccaatgc gcagcctgag agagaaaaac agcttataag tgactttaat
     1681 ccagataact tgacgggttt agctggcgat gttttcaaat ggatcgacag tcagtgcttg
     1741 ttccctagaa aaaacctttc cctggcggct gccctacaag ttatcggcaa tgcggcaggt
     1801 atgcgctatc gcgtagacag catatacaag acaacgctca acctcatgac gtttgctatt
     1861 gcagatagcg gatcaggaaa ggaggcggtc taccaagctg cctcatcgct attagatgct
     1921 gcaggtgtag gtctcgcagt ccatggcggc ataaagtctg agcaagagct ggtgcgcaac
     1981 gcggtgcgca accaggctag cctttacatg atcgacgagt ttggcgctat gctttcaaag
     2041 atcggcagcg caaaaaagaa gggcaacgct agctatcttg aagctgttcc cgctgagatt
     2101 atgaagattt tcaccaaagc aaatagcagc tacctagtgt ctggcgacat gcgcgaggag
     2161 atcgcagata gagcagataa gaaaatcgca tcaatccaga agcggctcga tgatggcagg
     2221 ggtggcgaaa aagcagagca ggagctggag cacgcaaaaa cagaaagaga acaaacaaac
     2281 agcgggatac ctaagccgtt tttatcgttc ttcggcatta gcgagccaaa cagttttgac
     2341 gccgcgatca ggatggaccc agatatgcta gtgaatggtt ttttaggtcg agcgctgctg
     2401 tttagagaaa gtgaagcatt accgatgcgt cgcagtgatt acaatccaag acagatgccg
     2461 atcacgctgt ctagccgatt gagccagctg tataaaaacg gctcagcagg cgcagagttt
     2521 gagcgggtgc aactttacgg cgatgaggaa acaataactg taagtgaaga cgctagagca
     2581 gagcttgaca gcgtttatgc gtactggaga acgagagggt tggagctaga gcatgagggg
     2641 caaggactgc acacgataac cacgcgcgtg tgggagatgg taatcaagat agctgggatc
     2701 ttatctatac catcagagat gggcagacag cctatcgtta cgcttgacaa tgttaggcag
     2761 gctcacgcga tcgttagaaa agttactgac tttaagataa gccactgcat aacaattatc
     2821 ggcgcagaca gcaaagacga tggtgagcgc gtagcaggcc tttttgaggg cgtgataggc
     2881 gtgctggaga gttacccgca aggaattgga gctggtgttc tgcgaaacag gaacaagaag
     2941 tacgggcgca ttaacgtaga caaatgtata gagaagctca ttgagcaaaa acgcgtaaaa
     3001 gagattgagt acactgatac gcgcaacagg tcacaaaaaa aatacaaact aatatgattt
     3061 tatttgttgc attattaatc tgctag
//
LOCUS       DASRMS010000223         1081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_61893, whole genome shotgun sequence.
ACCESSION   DASRMS010000223 DASRMS010000000
VERSION     DASRMS010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61893"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..748)
                     /locus_tag="VFC74_10665"
     CDS             complement(215..748)
                     /locus_tag="VFC74_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009289 - pilus [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HZK25830.1"
                     /translation="MRKIPFALILACGLASGSAFAATQGTVTIMGEVVDQTCTIVVGD
                     VDKTVTLATVSTTAFTAANQTVAKQSFTIGLENCEAGANVYAAFSAPVADIDVGTHTL
                     INKAAAGASNVNIILLEQNEGQILLGDPSYPPSTQPVPTATGDVTLEYSAAYYSTDAT
                     VTPGLVSAVANYVIAYQ"
BASE COUNT          346 a          261 c          206 g          268 t
ORIGIN      
        1 cttgcactaa agcgggcaca tcaccggtgt tgcttaactg aatattcacc tcacgctgat
       61 cggcaggata aatcactcgc gtaccggtaa tcaccacaga tgcgtgagaa aactgtacaa
      121 atgataacaa agcaacaaag accagcaaaa cacgcataga ctactccatc aagcaaaaag
      181 gggcccgaag accccttatg taaatcaact atgtttattg ataagcaata acgtagtttg
      241 ctacagctga cactagacct ggagtaacag ttgcatctgt ggagtaataa gcggcagagt
      301 attctaacgt aacatcccca gtcgctgtag gaaccggctg agtacttggc ggataggacg
      361 gatcgcccaa cagaatttga ccttcattct gttcaagcaa aattatgttc acattgcttg
      421 cacctgcagc tgctttgttt atcaaggtat gagtgcctac atctatgtca gctactggcg
      481 cagaaaatgc tgcatagaca ttggcacccg cttcacaatt ttccaggcca atcgtaaagc
      541 tctgtttcgc caccgtttga tttgcagcag taaaagctgt cgtagaaaca gttgccagag
      601 tgacggtctt atctacatca cccacaacaa ttgtacaggt ttggtcaact acttcaccca
      661 taatggttac cgtgccctgt gtcgctgcaa acgccgaacc actggccaaa ccgcaggcta
      721 aaattaaagc aaaaggtatt tttctcatct aaaactcccg ttcatataac taaatgtaag
      781 taacgcataa aaatgtggca actataatag ctagctataa taagccgaga tgcaatagct
      841 gactcatcaa gaaaatactt tcctattaat ccttaccatg tgtacatgat aggcacgttc
      901 tttaaaatat acaaactcaa tatcatataa gttcaaaaaa acccctcaca tctattgcta
      961 aatgtaagca agaaagctgt tgtctatagt acctagcgta acagcccgcc agacggcaat
     1021 cgtaggggta cagcgcgcgc aatcatcacc caacaatagc tgattatctg tccatctgaa
     1081 c
//
LOCUS       DASRMS010000224         1977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_401333, whole genome shotgun sequence.
ACCESSION   DASRMS010000224 DASRMS010000000
VERSION     DASRMS010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_401333"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="VFC74_10670"
     CDS             complement(<1..624)
                     /locus_tag="VFC74_10670"
                     /inference="COORDINATES: protein motif:HMM:NF012613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK25831.1"
                     /translation="MKTKKVVAAFLTLVMVLGSSAAIFAKDWSGQQSFSDVETSHWAH
                     GYVENLKARNIIGGYPDGTFRPDNLLKRSHTAKMIALAAVLNHENKTADFSDVPKDSE
                     FTAYIAALVDAGAVQGFTDGTFRPRNDIKRSHAAKIITKAFGLEIGTLEVNFLDLPSD
                     PVLANAINVLASNGIVKGYDNGNYFRPDDYTNRAQFAKMLCIAIAVAS"
     gene            complement(913..>1977)
                     /locus_tag="VFC74_10675"
     CDS             complement(913..>1977)
                     /locus_tag="VFC74_10675"
                     /inference="COORDINATES: protein motif:HMM:NF019192.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HZK25832.1"
                     /translation="AMLAQNPAVLNPIILGAADLRIPTGTTLDLLEGVTALDVFGNDL
                     SDRITADPETLDTSIAQTREVIYSVTDDYNRTAIKTTTIEVYKPEVLKGLLEGALAEG
                     KAIDLSLYTTSTAAALQAAITAADEVLADDEATQEQVDSAAAALMAAMNALILKPADG
                     PTPPPGPPAEDEPISPSFSDVTSDDWFFEYIESLYALGIVSGYGESGEFRPNNNLNRQ
                     EAAKMIAIAAGKKAGKGFVSKFTDVKVNSWAFEYIAALEEYGVVSGYKGTAEFRPLKN
                     IKRSHVSKMVVIAFGLDYGDVKKTFTDIQGADCEEFIKILASNGIVDGYKGTSKFKPD
                     DEISRAEFTKIIDLAMKIRG"
BASE COUNT          511 a          476 c          433 g          557 t
ORIGIN      
        1 ggatgcgacg gcaatggcga tgcaaagcat cttggcgaat tgagcccggt tggtgtaatc
       61 atcgggcctg aaatagttgc cgttgtcgta acctttaaca attccgttac ttgccaaaac
      121 gttgatggca ttagccagaa cggggtcgga aggcaaatca aggaagttaa cctccagtgt
      181 accgatctcg agcccaaaag cctttgttat gatcttggca gcatggctcc tcttaatatc
      241 gttacgaggc ctgaatgtgc cgtcggtaaa tccctggaca gcgcccgcat caaccaaagc
      301 tgctatataa gctgtgaact ctgaatcttt tggcacatca gagaaatccg cagttttatt
      361 ttcgtgattc aaaacggcag ccaaagcaat catctttgca gtatgggagc gcttcaagag
      421 attatccggc ctgaatgtgc catcaggata acccccaata atattccggg ctttaaggtt
      481 ttcaacgtaa ccgtgagccc agtggctcgt ctcaacatca gagaaactct gttgtcccga
      541 ccagtctttc gcaaaaatgg ctgcagaact tcccaaaacc attacgagcg tcaaaaatgc
      601 tgcgactact ttttttgttt tcactaccta gcacctcacc gtttctgaat caaaaaacta
      661 ctttttctct gctacacgaa gacacttaaa cgccaaaata acgtcttata tacataggta
      721 cagaaaacta taaaactacg caccctcgag aaaagaatat tttatggcaa atttgttggt
      781 ggaaatgttt atattagtat gctttaagga cttttgctga tgaaggcgcc taaaacgtat
      841 aacacgatgc taagccgaga gcgccaagtg cacgatatga cgttataatg ccttgtcgta
      901 atgcagtaat tgttatccgc gaatcttcat agccaaatca ataattttag tgaattcagc
      961 tctgctgatt tcatcgtcag gtttaaattt acttgttcct ttataaccat ccacgatgcc
     1021 gttgctggca agtatcttga taaactcttc gcagtctgcg ccttgaatat cggtgaatgt
     1081 ctttttaaca tcaccatagt ctagcccgaa ggctatgacc accatctttg acacatgact
     1141 gcgcttaatg ttctttaatg gcctaaactc ggcagttcct ttgtagccgg agacaactcc
     1201 atattcttca agagcggcta tatattcaaa ggcccaggaa tttactttca catcagtaaa
     1261 cttggataca aaacccttgc cggctttttt gccggcggct atcgctatca tcttggcagc
     1321 ttcttgcctg tttaagttat tattgggtct gaattcaccg ctttcaccat agccgctgac
     1381 tatccccaaa gcatagaggc tctcgatata ttcaaaaaac caatcgtcag aagttacgtc
     1441 cgaaaaagat ggacttatcg gctcatcttc ggcgggcggc ccgggcggcg gagtcggtcc
     1501 atcggccggc ttgagaatca acgcattcat ggctgccatc agagcagcgg cagcgctgtc
     1561 aacttgctcc tgcgtggctt catcatctgc caggacctca tctgcagcag taattgcagc
     1621 ttgcagggcc gccgcagtgc ttgtggtata taggctaaga tctattgctt tgccttcggc
     1681 taaagcgcct tcaagtaatc cttttaaaac ttccggtttg taaacctcga ttgtagttgt
     1741 ttttatagct gttctgttgt aatcgtctgt aacgctgtag attacttccc ttgtttgggc
     1801 aattgatgtg tcgagcgtct ccggatctgc cgtgatccgg tcgctaaggt cattgccgaa
     1861 cacatcgagc gctgtgacac cttccagaag gtcaagcgta gtacctgtag ggatgcgcaa
     1921 atctgcagct ccaaggatga tcggatttag cacggccggg ttttgagcta acatagc
//
LOCUS       DASRMS010000225         1000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_803022, whole genome shotgun sequence.
ACCESSION   DASRMS010000225 DASRMS010000000
VERSION     DASRMS010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803022"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..429)
                     /locus_tag="VFC74_10680"
     CDS             complement(<1..429)
                     /locus_tag="VFC74_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HZK25833.1"
                     /translation="MSIVEVKVPQFSESVSEGTLMEWNKQVGEAVSQDETLIEIETDK
                     VIMEVPSPADGVLVEIIVQSGEDVESEQLLAKIDTAATATAAAPAAEAKEEAAPAAAV
                     AANKGDVASPAAAKILSEKGLGASDVAGSGKDGRVTKADAQ"
     gene            complement(531..>1000)
                     /gene="kgd"
                     /locus_tag="VFC74_10685"
     CDS             complement(531..>1000)
                     /gene="kgd"
                     /locus_tag="VFC74_10685"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF028180.4"
                     /note="kgd; produces succinic semialdehyde; part of
                     alternative pathway from alpha-ketoglutarate to succinate;
                     essential for normal growth; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HZK25834.1"
                     /translation="SSPLKDFTEGEFKLVISETNPEINKEKVNRLLVCSGRVYSDLVS
                     NRAEREIDDIAIIRVEQLYPFPHKEFEAELQQYPNVKEIVWVQDEPQNQGPWFYIQHH
                     IYQNMAAGQKLSYAGRAASSSPAVGYLALHNEQLKTLLEQAFGPRLRGFSLTK"
BASE COUNT          262 a          232 c          206 g          300 t
ORIGIN      
        1 ttgtgcatcc gctttagtta cacggccatc tttaccagag ccggcaacgt cagaagcgcc
       61 taagcctttt tcagataaga ttttagcggc agccggagat gctacgtcgc ccttgttagc
      121 agctacggct gctgctggag cagcttcttc cttagcttct gcagcaggag cggcagcagt
      181 tgcagtggca gcagtatcga tcttggcaag taactgctca gactcaacat cctcaccact
      241 ttgtacaatg atttcaacta atacaccatc agctggggat ggtacttcca tgattacttt
      301 atcagtttca atttcgataa gtgtttcgtc ttgagaaact gcttcgccta cctgcttgtt
      361 ccactccatc aatgtacctt cggagacgga ttctgaaaac tgaggtactt taacttctac
      421 gattgacata attttgttcc tttaagctat gttttaactt taatagtttg cttttaaatt
      481 tttaaatcca aaacaggctt taacggcagt taaagcctga gtgcctaatg ctatttagtg
      541 agactaaaac cacgtaaacg cggtccaaaa gcctgttcga gcaatgtttt taactgctcg
      601 ttgtgcaatg ctaaataccc taccgctgga gaagatgaag cggcacgacc ggcataactc
      661 aacttctgac ctgcggccat attttggtaa atatggtgct gtatatagaa ccatggacct
      721 tggttttgcg gctcgtcttg tacccaaaca atttctttga cattagggta ctgctgtaac
      781 tcagcctcaa actctttatg agggaaagga tacagctgct caacacgaat aatagctatg
      841 tcgtctattt cacgctctgc acggttgctg actaaatccg agtaaacacg gcctgaacaa
      901 accaacaggc gattaacttt ttctttgtta atctcaggat tggtttcact gatcaccaac
      961 ttgaattcac cctcggtaaa gtcttttaat ggtgaggaag
//
LOCUS       DASRMS010000226         5350 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_402434, whole genome shotgun sequence.
ACCESSION   DASRMS010000226 DASRMS010000000
VERSION     DASRMS010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5350)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5350)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5350
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_402434"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            402..2084
                     /locus_tag="VFC74_10690"
     CDS             402..2084
                     /locus_tag="VFC74_10690"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HZK25835.1"
                     /translation="MKKQVMLSKLVIFMLIFLFLMSLAGCLQDTFSREVKHEQPLDSE
                     QERQRQGEEQRLAAERMLAERQQREADLQKELGAFFVPLPPLEIPENPVVKAKGLYVT
                     GHTAANSKRFAELLELIEETELNAMVIDVKNDNGLMTYRSEIEIVNEVNGNKGAVIED
                     IDAVLHELKARDIYPIARVVVFRDPHLPEVKQAWAIQKRNGGGQWRDPKDFAWVNPYE
                     KNVWDYNIAIAKEAALKGFREIQFDYVRFPENAKRVDREAEFPGSNDRRKDEAIEGFL
                     AYAREQLAEYNVHIAADVFGVIATSQGDAEGIGQNWEKVSSQVEYICPMIYPSHYGRG
                     YFGIAVPDANPAGTVTRALVDSLKRNALLEKPAIIRPWLQSFTATWVKGHIAYGPAEV
                     RAQIKAALALGIDEFLIWNAGNRYTPNSYLTADEANSLAADQAAERSAQGRDALGRTQ
                     QTALTDYLTAVQNKKWRDAFLWQSRDFAQSEAEYQTWVASWTSSLDSYVAAEQGAAAY
                     TVDVTFRLAETTKKLSGEIFTVVRENGVWKVQPSPAFLKSLTAEQDSDTAAE"
     gene            2245..2961
                     /locus_tag="VFC74_10695"
     CDS             2245..2961
                     /locus_tag="VFC74_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006570880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F420-0--gamma-glutamyl ligase"
                     /protein_id="HZK25836.1"
                     /translation="MTTAGAIAELKANPGRELTREVDGQLFNRYPIRTHFVEVGESYI
                     GLVEKYVLPYYQEGDILSMGEKVIALCQRQVKFKSEMKVSLLARILSRFAMKNPAGPA
                     MDNVYKMQTAIDLCGWWRVLFAAIFSAIGKLFGKHGVFYDFLGNNVRNIDGFCVVGWE
                     YYGDKGILAPAQPDRVCEEIKEKFGIDCMVVDANDLNIEILGHNKDIPYSKEFLADLI
                     RDNPAGQTQEQTPFILIRSK"
     gene            complement(3079..4170)
                     /locus_tag="VFC74_10700"
     CDS             complement(3079..4170)
                     /locus_tag="VFC74_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HZK25837.1"
                     /translation="MNSKTAILSSKTFLPDHFWRTYASFVVDSTRAAIVAIDINGQIT
                     LFNRQAELVFQKKRQDVIGASLASTFPQLDEHEHYLYLALKCERGMTEVENNYCPYTG
                     TEGVFIHSVAIVQNPSGEISGAIWMRKDQTSRRRFQNEINSAEVQALVSQIAAGTAHE
                     IRNPLTTTKGYIQLARQKTEQDVSAYLETALEGIEQIEEIITDLLSLFHPNIEGLQLT
                     SINSLLVELLQLVQHAGTLVNIVIRTILDKQAPLCLVDAKLIKYVLLNILRNAMEAMP
                     DGGCLTVKTAYHRETNTVTISVSDTGKGIKREDLRKIFDPFYTTKKEGSGLGLTLSNR
                     IMHHHNGYIDVDSTCGEGSCVHLHLPVTH"
     gene            complement(4327..4401)
                     /locus_tag="VFC74_10705"
     tRNA            complement(4327..4401)
                     /locus_tag="VFC74_10705"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4367..4369),aa:Val,seq:cac)
     gene            complement(4408..4483)
                     /locus_tag="VFC74_10710"
     tRNA            complement(4408..4483)
                     /locus_tag="VFC74_10710"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4448..4450),aa:Ala,seq:cgc)
     gene            complement(4508..5176)
                     /locus_tag="VFC74_10715"
     CDS             complement(4508..5176)
                     /locus_tag="VFC74_10715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HZK25838.1"
                     /translation="MKPANTGDRNQWTYSKRHLFTDRGFITIEGPVAKEIIEQFAICS
                     GLNQFRQPVQQQASLVEIAALPEGLVYLAHIHNTIVGYVTFHSPQFARWTNTGLDILE
                     LGAIEVSRYWRDMGIAAALIQIPFSTDFLEDKIIISLECYWFWDLRGKRINTWQYRQL
                     MQKLLEKGGFQTMITDDPDICDHPANLLSVRFGSLVPKEIFPLFESVCYQGKKENDPL
                     IFSP"
BASE COUNT         1533 a         1077 c         1267 g         1473 t
ORIGIN      
        1 cccgtttatg ctaatcctcc ccggccctgc tgttcaggcg gtccgctggg acaaaaaaca
       61 acatagagca agcagctaaa tcctcaagac tttttaatta acatcagtgc tggcagctat
      121 cagccagcac tttttgttat cttattttat agtaaaaatt aaagcaaaaa caattttaaa
      181 cggcgtaatt gcagctttaa gtgctaggaa gatcttaatg aaattaacta attgtttaat
      241 ataggataac taggggaagg tgctgattta tagccgaata cttattatat gtaagatagc
      301 tcagaaaaat gctagctaaa cacatattta cgagttcgac aggattcccc ccacccgcta
      361 gagtataagt atagctagaa tgcgaagagc gaggggatat gttgaaaaaa caagttatgt
      421 taagtaagct ggtaatcttc atgctcattt tcttattcct gatgagttta gctggttgct
      481 tacaggatac tttttcacgc gaagtaaagc atgagcagcc tttagactct gagcaggaac
      541 gtcaacgaca aggggaggag caaagattag cggcggaaag aatgctggca gaacgtcagc
      601 agcgtgaggc tgacctgcag aaggagctgg gtgccttttt tgtgccgctg cctcctttag
      661 agatcccgga gaatccggtg gtaaaagcaa aaggactcta tgttacaggt cacactgctg
      721 ctaatagtaa gcgttttgct gagttattag agctgattga agagactgag ttaaacgcca
      781 tggttatcga tgtcaaaaac gataatgggc taatgaccta ccgcagcgag attgagattg
      841 ttaatgaggt aaatggtaac aagggcgcgg taattgagga tatagacgct gttctccatg
      901 agctaaaggc gcgtgatatc taccctattg cacgtgtggt agtttttcgt gatccccatt
      961 tgccagaggt taaacaggcc tgggccattc aaaagcgaaa tggcggcggc caatggcgtg
     1021 atcccaagga ttttgcctgg gtaaatcctt acgaaaaaaa tgtctgggat tacaatattg
     1081 ccatcgctaa agaggcggct cttaagggtt ttcgtgaaat tcaattcgac tatgtacgtt
     1141 ttccagaaaa tgcaaagcgc gttgaccgtg aagccgaatt ccccggcagt aacgatcgcc
     1201 gcaaggatga ggccattgaa ggttttcttg cctatgcacg cgagcagctg gcggagtata
     1261 atgtgcatat tgctgccgat gtttttggtg tgattgctac ttcacagggt gatgctgagg
     1321 gtatcgggca aaactgggaa aaagtatcat cacaggtaga atacatctgc ccgatgatct
     1381 atcctagcca ttacggacgt ggatattttg gtattgccgt acctgatgcc aatcccgccg
     1441 gtactgtcac ccgggcccta gtcgactccc tgaaacgtaa tgccctgctt gaaaagccgg
     1501 caataattcg cccttggttg caaagcttta cggcgacttg ggtcaaaggt catatcgctt
     1561 atggtccagc agaagtgcgg gcgcaaatta aagcggcact agctctgggt attgatgaat
     1621 ttttaatttg gaatgcaggt aacagatata cccccaattc atatttaact gcggacgaag
     1681 ctaattccct ggcagccgac caagcggctg aacgttccgc gcaggggcgt gatgctctcg
     1741 gccgtacaca gcaaactgcc ctcacagatt atctgacagc tgtgcaaaat aagaagtggc
     1801 gcgatgcttt tctctggcag agtagagact ttgcgcaaag tgaagcggaa taccagactt
     1861 gggtcgcgag ttggacaagc tccttggact cctatgtggc agcagagcag ggggcagcag
     1921 cctatactgt tgatgtcact tttagattag ctgagacgac aaagaagtta agtggggaaa
     1981 tctttaccgt tgtcagggaa aatggggtat ggaaagtgca accttcccca gcatttttaa
     2041 agtcattaac agcagaacaa gatagtgaca ccgcagcaga gtaattaccc ggcggggcta
     2101 caagttggta atccgcctaa ggttttaagt ataatcttag ccaaattcgg atagagtaaa
     2161 ggttatgatt tctactcaat ttacagcaga ttactgcagg atttcgctta cacaggtcga
     2221 atcaatactg ttgaggtgag caaaatgaca acagcagggg ctatagctga actaaaagct
     2281 aatcctggcc gcgaattgac cagggaggta gatggccaat tatttaacag ataccctatt
     2341 cgtacccact ttgttgaggt tggagaaagt tacattgggc ttgtggaaaa atatgtactt
     2401 ccctattatc aggaagggga tattctttcc atgggcgaaa aagttattgc tttatgccaa
     2461 aggcaggtta agttcaaaag tgagatgaag gttagcttac ttgctagaat tctttcacgc
     2521 tttgcgatga agaatccagc cgggccggca atggataatg tttataaaat gcaaacggca
     2581 atagatttat gcggctggtg gcgagtatta tttgcggcta ttttttccgc aattggtaag
     2641 ttatttggca aacatggtgt tttctacgat ttccttggta ataatgtgcg caatattgat
     2701 ggtttctgtg tagtgggctg ggagtattat ggtgataaag gtattctagc accagctcaa
     2761 ccggaccgcg tttgtgagga aattaaggaa aaattcggta tagactgcat ggttgtagat
     2821 gcaaacgacc ttaatatcga aatcctcggg cataacaaag acattcccta tagcaaagaa
     2881 tttttagctg atttgattag ggacaaccct gctgggcaaa cccaagaaca gacacctttc
     2941 atcttaattc gcagtaaata aataaaatca tcgcgaaaaa gtagcgcaag cgcgctattt
     3001 ttttgcagaa aaacttgctc aattactaaa ggctgcactt gctagtgaaa aagaaaaaac
     3061 ctgcgcaagg caggtctttt aatgggtgac gggtaggtgt aagtgtacac aagaaccttc
     3121 cccgcaggta ctgtcgacat caatataacc gttgtggtgg tgcataatgc gattggagag
     3181 ggtaagtccc aaacccgaac cttctttttt tgtggtataa aagggatcaa atattttgcg
     3241 caaatcttct cttttaatgc ctttacctgt atcagaaacg gagatcgtta cagtattcgt
     3301 ctcacggtga tatgctgttt ttacagttag gcagccacca tcaggcatgg cttccatagc
     3361 attacgtagt atatttaaga ggacgtactt aattaattta gcatccacta aacataaagg
     3421 tgcctgttta tcgaggatag tacggatcac aatattgaca agcgtgccag cgtgttgtac
     3481 taactgcagc agttctacga gtaaggagtt gatggaggta agctgtaatc cttctatgtt
     3541 ggggtgaaaa agtgataaca aatctgtgat gatctcttca atttgttcaa taccttctaa
     3601 cgccgtctct aaatatgccg aaacatcctg ctctgttttt tgcctagcga gctgaatata
     3661 tcctttagtt gtcgtcagtg ggttgcgtat ttcgtgtgct gtgccagcag caatctggct
     3721 gacaagcgcc tgaacttctg cggaatttat ttcgttctgg aaacggcggc gacttgtctg
     3781 atccttgcgc atccaaattg caccgctgat ttcgccagat gggttttgca cgatagccac
     3841 actgtgaata aaaacacctt ctgttccagt gtagggacaa tagttgtttt ctacttccgt
     3901 catgccccgt tcacatttta aggccagata tagatagtgt tcgtgctcat caagttgggg
     3961 aaaagtactt gctagagagg cgccaataac atcctgtctt tttttctgaa agacaagttc
     4021 tgcctgtctg ttaaacagtg taatctgacc gtttatgtcg atagccacaa tagctgcccg
     4081 agtactatca acgacaaaac tagcgtacgt ccgccaaaag tgatcaggta agaaagtttt
     4141 agagcttaaa attgctgttt ttgaattcaa tgcaaagccc ccccttggca aaaatagcga
     4201 acaatatcaa ttatagcaga ttaacaatga aaaatttagc agtttatgtc gtaaaactaa
     4261 aatatatttt agtttttatt aaaaaaacca cgaacgctga tgacgttcgt ggctactgga
     4321 attttatggt ggggctaact gggatcgaac cagtggcctc ttgaatgtga gtcaagcgct
     4381 ctccccctga gctatagccc cattagatgg tggacgtgag gggattcgaa cccctggcct
     4441 cttgaatgcg agccaagtgc tctcccgact gagctacacg cccttaatct gaacttattt
     4501 tatagcatca aggcgaaaaa atcaagggat cgttttcttt tttcccctgg tagcaaactg
     4561 actcaaaaag aggaaaaatt tctttaggca ctaaggagcc gaagcgtaca gatagtagat
     4621 tggcaggatg atcacagata tcaggatcgt ctgtgatcat ggtttggaag ccgccctttt
     4681 ccaatagttt ttgcattagt tggcgatact gccaggtatt aatacgtttg ccgcgtaaat
     4741 cccaaaacca gtaacactcc agtgaaataa taattttatc ttccaagaaa tcggtagaaa
     4801 aaggtatttg tattagagct gccgcaatgc ccatatcccg ccagtaccgg gatacttcaa
     4861 tcgcccctag ttccaaaata tctaatcctg tattggtcca acgtgcaaat tgcgggctgt
     4921 gaaaagttac atagcccaca atggtgttgt ggatgtgggc aagatagacc agcccttcag
     4981 gtagggcagc tatctctact aatgatgctt gctgctgcac tggttgtcta aactgattta
     5041 agccgctgca aatggcaaac tgctcaataa tttccttggc aacagggcct tcaatggtaa
     5101 tgaagccgcg gtctgtaaat agatgacgct ttgaatatgt ccactggtta cgatcgccgg
     5161 tgttggcagg tttcatcacg tcacctcatt tatttttctt agttatatta tgattagccc
     5221 acaaaaagca ggaacataat gacaaattga acactttctg gtataatgga gatagtagat
     5281 atgccggaaa ggcggtgcgc tgtaatgttt tgtaggaatg aaactcagca aacgacgata
     5341 atggatccac
//
LOCUS       DASRMS010000227        21634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_935457, whole genome shotgun sequence.
ACCESSION   DASRMS010000227 DASRMS010000000
VERSION     DASRMS010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 21634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21634
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935457"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFC74_10720"
     CDS             <1..781
                     /locus_tag="VFC74_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006521792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HZK25839.1"
                     /translation="LDVVLRVVPFEVPQVDELGLPAVVKEFSRLPRGLVLVTGPTGSG
                     KSTTLAALIGMINEERSMNIVTVENPIEFLHTHNKSIVRQREVGTDTQSFATALRHML
                     RHDPDVILIGEMRDMESISIALTAAETGHLVFSTLHTQTAPLAIHRIVDVFPEGVRDY
                     IRLQLADSLKGVISQQLLPRADGHGRVAAVEVLVNTPAVSNMVREGNEHQLYTAMQTA
                     RATGMQTMDSALADLCLRGLVTKEEALSRSVNRTELERALR"
     gene            781..1533
                     /locus_tag="VFC74_10725"
     CDS             781..1533
                     /locus_tag="VFC74_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HZK25840.1"
                     /translation="MLLPALAFIYGLFIGSFLNVCIYRLPAGESIVTPPSHCPNCGRR
                     LGARELLPVVSFLWQRGRCRGCGSAIAWRYTLVELATAILFAVLAWRFGWPDILWQAA
                     FYAVLVVIFFIDLDHQLIPDQLVLLLLGITVLVLVLERGVPWGTALLGGLVGFGLFLL
                     LAVLSSGGMGGGDIKLAAVLGLWFGWPQLLLVMFLSFLGGGLVGGALLLLKLKNRKDG
                     IAFGPFLVLAAFIVSMWGNQLITWYLRIAGFI"
     gene            1624..2001
                     /locus_tag="VFC74_10730"
     CDS             1624..2001
                     /locus_tag="VFC74_10730"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HZK25841.1"
                     /translation="MHKKSRGFLLVELLVTLALLAMVAPPLLNLVLLGGQTLTIAYRQ
                     TVAINLAREALEWQRSAGFCGSENWLEEEVIGFREFSRELKVTRLPLTEPAWPLKAIC
                     VTVSWQEGSRRQKVQVVTWQTWR"
     gene            2014..2481
                     /locus_tag="VFC74_10735"
                     /pseudo
     CDS             2014..2481
                     /locus_tag="VFC74_10735"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
     gene            2478..3521
                     /locus_tag="VFC74_10740"
     CDS             2478..3521
                     /locus_tag="VFC74_10740"
                     /inference="COORDINATES: protein motif:HMM:NF025699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HZK25842.1"
                     /translation="MTRTRGSALLWLIFTVLLFTLISCSLLKIALGDLRVSGHLVNAE
                     RAQYAAEAGIEYAAAILPWQCRSLAAGWQYQQTEPPEFAVTAEAESSSLLRIHAQGRA
                     GGMVQDVEVLASLRPLGKQALITQQLLAQDLTVTGHVAADEVVFRETPTYIAGDLRTA
                     WLEEQDGGTYHCSGYLWGDIRPYALEVDFIGLAQEAALWGWTEPDCAGEVYQVDGSQV
                     GPLFAPGKTAIALQLPWEGLIVALGEVTLQTWVPGSKVMLVAAGEVELAGVDEVWEGS
                     LFIYSGEKIYCDPGGQLQLAGCLVSPLLELTGLKITYDDQTVLNQLELVPAELLQVAP
                     TFDLQWLELAPRR"
     gene            3518..3961
                     /locus_tag="VFC74_10745"
     CDS             3518..3961
                     /locus_tag="VFC74_10745"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HZK25843.1"
                     /translation="MKKHGFSLLELVMVVVILGILLGAAVPAISNLGQWPLKTAAQEL
                     AAAMREARETAIAEGQTCTLVFYDIGGRYRLDLPAGTIWTTLPEELQMIANFPAVDNR
                     ATLYYRYTGAPNRGGYVRLQDQAGRRLYVIVTPVTGRVRLDSAPP"
     gene            4125..4640
                     /locus_tag="VFC74_10750"
     CDS             4125..4640
                     /locus_tag="VFC74_10750"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HZK25844.1"
                     /translation="MRSIAGKNIVLIGFMGTGKTEVGKLLAASLNRRLVDTDQTIVET
                     EGRPITEIFAQEGERYFRRLETAVIEQLCQQRGLVIPTGGGAILDPHNVALLQASGLV
                     IWLDASVAELERRLAHDQSRPLLTGETGVGELYQQRQAAYRQAAHERVDTTAKPPLAV
                     VAEIMAIIRKN"
     gene            4780..5844
                     /locus_tag="VFC74_10755"
     CDS             4780..5844
                     /locus_tag="VFC74_10755"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011195993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HZK25845.1"
                     /translation="MDRLNVLRKQLQLEGREAILVANPINVAYLSGFTGTAGSLLITN
                     SQAYLLTDFRYVEQAQQQAPAFETIDTKNKLWPLAADLLADNACQTLTVEADYLTVDT
                     YEEVVTGLAAIAVEAAASPVTPIRVVKTAEEIKAMEKAQKLTDAAFSQILEQIKPGVR
                     ERELALELEFMMRRGGASGLSFTMIVGSGSRSALPHGVASEKILAVGDAVVLDYGCIV
                     DGYCSDMTRTVFVGQASDKQREVYQLVLAAQEAALAKLQAGVSGRDGDAFAREVITAA
                     GYGEYFGHGLGHGVGREIHESPRLSASYEGLLEPGMIVTVEPGIYLPGEFGVRIEDMV
                     VIEEQGIRNLTASSKELYCL"
     gene            5860..6417
                     /gene="efp"
                     /locus_tag="VFC74_10760"
     CDS             5860..6417
                     /gene="efp"
                     /locus_tag="VFC74_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HZK25846.1"
                     /translation="MISTSDFRTGLTIELDGVVFVIVDFQHVKPGKGAAFVRTKLKNA
                     KTGSTTERTFRAGEKMERAIMDRRAMQYLYNSGDEYHVMDTETYEQLALTGEQLGDGL
                     KYLKENMEISVLFYQEQVFGVEVPNFVELEVAETEPGIKGDTASGGSKPATMETGLVV
                     QVPFFINAGDVLRIDTRSGEYMERV"
     gene            6451..6915
                     /locus_tag="VFC74_10765"
     CDS             6451..6915
                     /locus_tag="VFC74_10765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25847.1"
                     /translation="MDDLTSRVAYLKGLAAGLGFDPGSREGKLFTQLIDVIDALAESV
                     SELQEDYVDIVEYAEAIDEDLNDLEEDFYDEDELLDDDEDFDDEEFDEDDMFSVECPE
                     CHEIVYIDDDMLEDDDVVEILCPNCERVVFVNDDRDFDDFQDLEVLDEEGQE"
     gene            7121..8116
                     /gene="spoIIIAA"
                     /locus_tag="VFC74_10770"
     CDS             7121..8116
                     /gene="spoIIIAA"
                     /locus_tag="VFC74_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AA"
                     /protein_id="HZK25848.1"
                     /translation="MKDENWQEQILPLLPPQLRAIFTSLPAALAASLEEIRLRAGQPL
                     MVQSGRGEVWLGLAGPVTALGESCRITAADLQHILLQMTEYSLYARDEELKRGYLALP
                     GGHRAGFAGRVVMEGNQVKLLRDINALNIRIARQFKGAALKILPHVYCDKTQRVRHTL
                     LVSAPQAGKTTMLRDLARLLANGDQQKGRPGFKVGIVDERSELAGCFRGEPQLDVGVR
                     SDILDGCTKAEGMMMLVRSMSPQVIVTDELGRAEDARAVEEAVNTGASILATAHGQNA
                     RELIRRPSLAYLLENGLFERVIVLSRRHGPGTVEDIYTGHSLQEWLRAGRECNVD"
     gene            8106..8630
                     /locus_tag="VFC74_10775"
     CDS             8106..8630
                     /locus_tag="VFC74_10775"
                     /inference="COORDINATES: protein motif:HMM:NF021090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AB"
                     /protein_id="HZK25849.1"
                     /translation="MWIKWLGAVLILAAALMWGNLQAAKLRARVRELEEFRLALRLLA
                     AEIGYTATPLPRALEQVGKRLGNQGVQAFLAAVRQQLQDPQTADASTAWLAAVHSVKP
                     LLCLTPEDWAVVARASAGLGGLGRDDQLKQLAAAETQLAAQAVEAAALCASNEKMWRY
                     LGALSGLTVVILLL"
     gene            8661..8867
                     /gene="spoIIIAC"
                     /locus_tag="VFC74_10780"
     CDS             8661..8867
                     /gene="spoIIIAC"
                     /locus_tag="VFC74_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AC"
                     /protein_id="HZK25850.1"
                     /translation="MNGFNVDLIFRIAGIGIFISVLNIVLKQAGKEEQAQMLTLGGVV
                     VVLLMVIQLINELFSSIKTIFNLY"
     gene            8913..9299
                     /gene="spoIIIAD"
                     /locus_tag="VFC74_10785"
     CDS             8913..9299
                     /gene="spoIIIAD"
                     /locus_tag="VFC74_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AD"
                     /protein_id="HZK25851.1"
                     /translation="MEIIQIVAFALIATFMVVVVRETKPVYAMLIAVAAGVIIFLRVA
                     VYLSTVLQMLLDMTLRANISLVYLNTLLKILGIAYLAEFGAQICRDAGESIIAGKVEF
                     AGKMLILVMAMPLLVAVLETIIKFVP"
     gene            9307..10476
                     /gene="spoIIIAE"
                     /locus_tag="VFC74_10790"
     CDS             9307..10476
                     /gene="spoIIIAE"
                     /locus_tag="VFC74_10790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0030436 - asexual sporulation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AE"
                     /protein_id="HZK25852.1"
                     /translation="MKKIGTVILLTLCLWLTTSPVLAKPATELVQQQAQELEMTEIES
                     FWEELQRESGEYLPDFTWHNILEWFRPGEGQGLSFSEIINGLWRFMWREIYFNLNLLA
                     KLLFLAVIAALLKNLEKAFANDSISALTQAVVYLALVSIAMQSFAVAIGIGRDSVNNM
                     VELILAVIPLLLALLASLGSFASAALFRPIIIFAVNFFAALTRDVVFPLIFLNTILSI
                     VNYFSPRVTVSKLADLLKNSAVWTMGLCMTIFTGLMSIYGVASTVGDAVSLRTAKFLT
                     SSFLPVVGGMISDAVETVAGATLILKSTVHIAGILVLFYLVAFPLIKILVLVFIYKLA
                     AALIQPLGETSLCDSLNTLGNCLALVFAAVAVVSVIFYFALTVISGAGNASFMLR"
     gene            10485..11081
                     /locus_tag="VFC74_10795"
     CDS             10485..11081
                     /locus_tag="VFC74_10795"
                     /inference="COORDINATES: protein motif:HMM:NF021121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage III sporulation protein AF"
                     /protein_id="HZK25853.1"
                     /translation="MLAVLSEVVRNIVILIVLVTILEMMLPRKEFRPFVNMVVGLVLM
                     LMLLAPLRSLLQLPGLLDPVVEMRDALTEETIARQQAEIEQMNWDLTLVRYRQLVEEK
                     VAQLLREADLEIVSMDLQVEEDVNHLEFGVPRQISVVAQPLQPPAGLIEPVEPIEVGI
                     GEGELVPGGGVYEAEFAAQIAAALGISAELVQVRVIYH"
     gene            11095..11697
                     /locus_tag="VFC74_10800"
     CDS             11095..11697
                     /locus_tag="VFC74_10800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25854.1"
                     /translation="MSEQFWQRVLDKVQGRGDKDGKKQYSTWFIVALVAVGIYFMYIS
                     AMPDKSSGEQPLIQPGGGAAVTSFKSDYKDQVERQLSAELQSVKGVDAVRVFVTLASG
                     SEYEYIHNQETTNRKTEEEDSGGGNRIIEEETTKTQAVMQQTGSGQQAVVVREKTPEI
                     AGVLVTVKGDGSARLLEDVTLAVRAALYLPAHRVKVLEME"
     gene            11712..12344
                     /locus_tag="VFC74_10805"
     CDS             11712..12344
                     /locus_tag="VFC74_10805"
                     /inference="COORDINATES: protein motif:HMM:NF024097.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIIAH-like family protein"
                     /protein_id="HZK25855.1"
                     /translation="MERETSRKVTTIVLLVLALAAVWYAKQFQNDRIDIVDPAKQSET
                     EDPLGQGEPAAEEEPGTGEGENEGDSEGDSEETTAPVVTAPESMGLESYEAYFVEYRL
                     QRDRIRASEVEMFNTMIENPNVSAEAKKEAEAQLLSLIERMEKELMVENMLKAQGFKD
                     AVFFIKDGKAHAVVKAAKLDQTQFMQIAEVVNSVTGLSMDDISVVEHGGS"
     gene            12442..12843
                     /locus_tag="VFC74_10810"
     CDS             12442..12843
                     /locus_tag="VFC74_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HZK25856.1"
                     /translation="MSAEEEFIPTELGTIKIADDVVAIIAGLAATEVEGVAGMSGGLA
                     GGIADMLGKRNLAKGVKVEVSNQQALIDIFIIVKFGARIPDVAWKIQESVKKAIESMT
                     GLKTASVNVHVQGVSFDTAKGEEQEEDLQIR"
     gene            12927..13460
                     /gene="amaP"
                     /locus_tag="VFC74_10815"
     CDS             12927..13460
                     /gene="amaP"
                     /locus_tag="VFC74_10815"
                     /inference="COORDINATES: protein motif:HMM:NF033218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HZK25857.1"
                     /translation="MNTRERLYLVIFAVLLSAMAVLVGGAALSLYPLEVLQDTVGAVY
                     GHLEYAILAVLLLALAVWAFLTSVRRKNTVESLAQPGPLGEVSISFKAIENLVLKATR
                     NVNGVRDIKTRIVYTESGLVIYLRATTLPDEKIPELTAGLQAAVKEYVESSTGSTVAE
                     IKVLIENVVTDTIKATR"
     gene            13477..13698
                     /locus_tag="VFC74_10820"
     CDS             13477..13698
                     /locus_tag="VFC74_10820"
                     /inference="COORDINATES: protein motif:HMM:NF021542.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HZK25858.1"
                     /translation="MPAKTLRQFIVEHWGKILGGALGLIVGLCILLLGFWRSVLLFLC
                     IAVGVYLGRLFDRHEGIQGILQRIWPDND"
     gene            13712..14131
                     /gene="nusB"
                     /locus_tag="VFC74_10825"
     CDS             13712..14131
                     /gene="nusB"
                     /locus_tag="VFC74_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013810553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HZK25859.1"
                     /translation="MSRRAAREVAFKTLFQYEQGKNSIEPSLSELLTESGLNPQSALF
                     ARELVNGTVDKQEEIDQTISPYLQNWQLERLAAVDRSVLRLAAFELLYREDIPPAVSI
                     NEALELCKAYSGEESAKFLNGVLDKLAHTKGGSKEEN"
     gene            14131..15084
                     /locus_tag="VFC74_10830"
     CDS             14131..15084
                     /locus_tag="VFC74_10830"
                     /inference="COORDINATES: protein motif:HMM:NF013012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-sialoglycoprotein endopeptidase"
                     /protein_id="HZK25860.1"
                     /translation="MFLGLDTSCYTTSLAVIDTQGRLLSEARQLLKVPQGERGLRQAN
                     GVWQHLQNLPQLAQQLQTEIGPLRLQAVAASSQPRPVAGSYMPVFMAGLSLARSLAAL
                     AGVPCIELSHQEGHLWAGLWSAKVRWDDFYALHVSGGTTELLSVKQAERLQIVELGGS
                     ADLQAGQFIDRVGVAMGFSFPAGPQLEALAQTAGGEIAQVPVSVQGFNMSFSGPESHV
                     QRLLAGGSVNQAAVARGVELCIAESLRRVIINARQQQGEKPVLLVGGVMANKFIRTFL
                     AEQLDGHCAFAQPRFAGDNAVGAALYAQQAGKKIKLANRKR"
     gene            15168..16394
                     /gene="xseA"
                     /locus_tag="VFC74_10835"
     CDS             15168..16394
                     /gene="xseA"
                     /locus_tag="VFC74_10835"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HZK25861.1"
                     /translation="MASQNKRIFTVGQLTQYLKNIFAADPQLNRLRVSGEISNFKHHS
                     SGHMYFTLKDAQAAVRCVMFRSANARLTFPPANGVKVVASGKLSIYERDGQYQLYVDS
                     LLPEGVGSLFAAFEALKAKLAAEGLFEAAGKQELPRFPQRIGVITSPTGAAIRDIVTT
                     ITRRFPPAEILIVPVLVQGPEAPRQIAEAIDFLNKVPALDVIITGRGGGSIEELWAFN
                     EEIVARAIFASQIPVISAVGHETDFTIADFVADVRAATPTAAAELAVPEQRELQQFLE
                     TSQNRLLLKLRTKLGQERRYLEQLAASRVLTRPLERVEQAKQTLDNLSSRLETQMVYH
                     LQNQTAKLQLLSGKLGALNPEAVLERGFAICYDQAGQVVRAADQLQTGDAVEIRLRRG
                     RASAEILQTKTEEENE"
     gene            16387..16641
                     /gene="xseB"
                     /locus_tag="VFC74_10840"
     CDS             16387..16641
                     /gene="xseB"
                     /locus_tag="VFC74_10840"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HZK25862.1"
                     /translation="MSKKTTPIAGLNFEEALAKLEQVLQQLERSDCPLEEALALFQEG
                     MQLVQHCRTKLNHAEDKISILLKEAEEFVPFNGEGGSHDL"
     gene            16631..17512
                     /locus_tag="VFC74_10845"
     CDS             16631..17512
                     /locus_tag="VFC74_10845"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004337 - geranyltranstransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="farnesyl diphosphate synthase"
                     /protein_id="HZK25863.1"
                     /translation="MTFKPAEVIVEVNEALQQFLPPPTTFPTEIHRAVHYSVFAGGKR
                     LRPLLTLAAADIFTVPRQHALPAACAIEMIHTYSLIHDDLPAMDNDDYRRGRPSNHKV
                     FGEGMAILAGDALLTLAFETLATKASAYFPAETVVALQQELSQASGMAGMIGGQVVDI
                     VSEGQAANAEILDYIHRHKTGALFRCCLRCGALMGQAAAAELAALTDYAEKIGLAFQI
                     VDDILDITGEAAALGKQTGSDANKQKMTYPALYGLQQAQAKADTLLEEALLLLQPFGA
                     AAGSLQALTLMLVQRHS"
     gene            complement(17707..18096)
                     /locus_tag="VFC74_10850"
     CDS             complement(17707..18096)
                     /locus_tag="VFC74_10850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25864.1"
                     /translation="MALSKDKKKQAICIKCGEAGLYLIPTQHHVYIECRSGHAWQEKY
                     LDQGGTITRPHTAVECIEDLFTPEEKILYERITTELEEHTDYYKNADTLEKVMHLCQK
                     CQASEQEIYTIFKIITLYQRAMETTQN"
     gene            complement(18077..18349)
                     /locus_tag="VFC74_10855"
     CDS             complement(18077..18349)
                     /locus_tag="VFC74_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25865.1"
                     /translation="MSNDHGAAPAYNFLINASNQANLAGTQELNATQINSVFVLVTPY
                     YIDRFNLAGLNLDIRIDEEGILWVDQQAFEPIEIVRGVKVIGIIQR"
     gene            complement(18386..18658)
                     /locus_tag="VFC74_10860"
     CDS             complement(18386..18658)
                     /locus_tag="VFC74_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25866.1"
                     /translation="MQKSKLVYLSNLSNEANMCQARTTTLYQLNVGVLIGVDYLRESA
                     LAKQKIPLAITIDKMGDLTIEGIDGITINDEHLRMHSDRKITDSEE"
     gene            complement(18645..18836)
                     /locus_tag="VFC74_10865"
     CDS             complement(18645..18836)
                     /locus_tag="VFC74_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25867.1"
                     /translation="MSERKIKTPELEVSLDLPNWAYSPLLEELYQLRLNTELTNEEKT
                     AKLQSILHTMLKDDQDAEE"
     gene            complement(18847..19131)
                     /locus_tag="VFC74_10870"
     CDS             complement(18847..19131)
                     /locus_tag="VFC74_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25868.1"
                     /translation="MKNPWITIKPLRHKKIPEIIIYKSGQVLLEGEVFKEDDPRYRNL
                     SVNIEAHNKLNTGEETLQMLALQKEAEKGETITVSESKDKNKSEQTTIKF"
     gene            complement(19145..19324)
                     /locus_tag="VFC74_10875"
     CDS             complement(19145..19324)
                     /locus_tag="VFC74_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25869.1"
                     /translation="MTEESKQPARQRQNDVFQNDVFMEAEIIDQLNTNQQSNMTLSTE
                     SVITQTESMLKLNLT"
     gene            complement(19528..19683)
                     /locus_tag="VFC74_10880"
     CDS             complement(19528..19683)
                     /locus_tag="VFC74_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25870.1"
                     /translation="MPDVLLASVVNQKVVNQNAQQILVLVAAQIVNVLEAQFGFSTNL
                     DNDTINS"
     gene            complement(19712..19924)
                     /locus_tag="VFC74_10885"
     CDS             complement(19712..19924)
                     /locus_tag="VFC74_10885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25871.1"
                     /translation="MKIGFTRKGLTLDSKPFNPLDFSVNGYGIESTDEPMQFDAFEIL
                     EKLAGARGEGVTREEQVKVLQSIIPK"
     gene            complement(19944..20183)
                     /locus_tag="VFC74_10890"
     CDS             complement(19944..20183)
                     /locus_tag="VFC74_10890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25872.1"
                     /translation="MKKFAYQYATGTIPQKTVRQFGAVDFFAASVINQKAINQNAQQI
                     LVLVAAQVLNITEAQFAFATNEDQDVVQGGIDNLL"
     gene            complement(20214..20393)
                     /locus_tag="VFC74_10895"
     CDS             complement(20214..20393)
                     /locus_tag="VFC74_10895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005587456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25873.1"
                     /translation="MSRQFTGDFFSASVINQKAVNQNTQQILVLVAVQVANLIEAQFG
                     FATNDDSDTIEGLAI"
BASE COUNT         5327 a         4367 c         6100 g         5840 t
ORIGIN      
        1 gctggacgtg gtgctgcggg tagtaccctt tgaggtgccg caggtggatg agttaggact
       61 gccagcggtg gtgaaagagt tttcgcggtt gccccgggga ttggtactgg tgacgggacc
      121 gacgggtagt ggtaaaagta ctaccttggc tgccttgatc ggtatgatta atgaggaacg
      181 gtccatgaat attgtgactg tggaaaaccc cattgaattt ttacatacgc ataataagtc
      241 tattgtgcgg cagcgtgaag tggggacaga tacgcaatcc tttgcgacgg cgctgcggca
      301 tatgctgcgc catgacccgg atgttatttt aattggtgaa atgcgggaca tggagagtat
      361 ttctattgct ctgacggcag cagaaacggg ccatttggtt ttttctactt tacatacgca
      421 aactgcacca ctggctattc accggattgt cgatgtcttt ccggaaggag tgcgcgacta
      481 tattcgtctg cagttggctg attccctgaa aggtgttatc tcgcagcaat tattgccccg
      541 tgccgatggc catggccggg tcgcggcggt ggaagtgctg gttaacacac cggcggttag
      601 taacatggtg cgggaaggaa atgagcatca gctttataca gcgatgcaga cggcacgtgc
      661 gactgggatg cagacgatgg atagtgcgct ggctgattta tgcctgcgtg gtctggtcac
      721 taaagaggaa gcattatccc gtagcgtcaa taggactgaa ttggaaaggg cgctgcgtta
      781 atgcttttgc ccgctttggc ttttatctat ggtctgttta ttggttcttt tcttaatgtt
      841 tgtatttatc gtttgccggc aggggagtcc attgtcacac ctccttctca ctgtcccaac
      901 tgtgggcggc gcttaggggc gcgggaactg ctccccgtgg tcagcttttt atggcagcgc
      961 gggcgctgcc ggggctgtgg cagcgctatt gcctggcgct atactttagt ggaattagca
     1021 acagcgatcc tgttcgccgt cttagcctgg cgttttggct ggccggatat tttgtggcag
     1081 gctgcttttt acgcagtgct ggttgtgatt ttctttatcg atctagacca tcagttaatt
     1141 cccgatcaat tagtgctcct tttattgggc atcactgtgt tggtcctggt cttagaacgt
     1201 ggtgtgccct ggggcacggc cttgctgggc ggcttagtgg gttttggctt gttcttactg
     1261 ctggcggtgc tgagtagtgg cggcatgggc ggtggtgaca ttaaactggc tgccgtttta
     1321 ggtttatggt ttggctggcc gcagctgttg ttagtaatgt ttttgtcctt tctcgggggc
     1381 ggcctggtag ggggtgcgtt gttgctatta aaactgaaaa atcgtaaaga cggaattgcc
     1441 ttcggtcctt ttctggtgct ggcagctttt atagtcagca tgtggggcaa tcagcttatt
     1501 acgtggtatt tacgcatcgc aggttttatc taaaacttta gacaagtaaa atgcaagcac
     1561 cagatactct ggtgcttttt tggcgtttct gtgtcgaaaa tttgtgagga gaagggatgg
     1621 gtgatgcata aaaaaagcag ggggttctta ctggtcgagc tgttggtaac gttggctttg
     1681 ctggcgatgg tagcaccacc gttattaaat ctggtgctgc tgggaggaca gacattaacg
     1741 atagcttatc gacaaacggt ggcaattaat ttagcacgtg aagccttgga gtggcagcgc
     1801 agtgcaggct tttgtggcag cgaaaattgg ttagaggaag aggtgatagg atttagggag
     1861 tttagccgtg aacttaaggt aacgcgtctg cccctgacag agccagcttg gccgctcaag
     1921 gcgatttgtg tgacggtcag ctggcaggaa ggcagcagac gtcaaaaggt gcaggtagta
     1981 acttggcaga cttggaggtg aagaaatggg gaaaatggtt ttacgctgat ggagttacta
     2041 gtggcagttt ctttgctggt ggtggtgttg acggcggtct taaccatctt accatggggc
     2101 ggtcagggct gggaaagaca gacgacgcag tccaacgggc agcagcatgc ccgcatcgcc
     2161 atggaagaat tggttaatga gttgttatat gcataccgca ttgaagtcaa cccagtcatg
     2221 gggatgatta catattacaa agaaaatagg ggcgatctgc agcgttatcg tgtttatctt
     2281 ttgggagagc agctgttgtt ggatttaccg gagggaactg cggtgccgct ggccagctgt
     2341 attgaaggca tcatcctgca tccggcagga acgctgtacc caggggatgt gctgacctta
     2401 actttgcagg ttaaccagca ggtgaggcat gaagtgagaa ctgctgtggt ggccaagaat
     2461 ctcacaggtg aagccttatg actagaacgc gcggttctgc tttgctgtgg ctgatcttta
     2521 ctgtgttgct ctttacgctt atcagctgct cactattaaa gatagcgctg ggcgatttgc
     2581 gagttagtgg gcatttagta aatgccgaac gggcccagta tgccgcggaa gctggtattg
     2641 aatatgctgc ggcgatcttg ccttggcagt gccggtcact ggcggcaggt tggcagtatc
     2701 agcagacaga accgccagaa tttgcagtta cagctgaggc agaaagcagc tctttactgc
     2761 gcatccatgc ccaaggaaga gcaggaggga tggtgcagga tgtggaagtg ctggccagct
     2821 tgcgtccttt gggcaaacag gcgctgatta cccaacagct gctcgctcag gacttgacag
     2881 tcacgggaca tgtggccgca gatgaggtgg tgttcaggga aacgccaacc tacatcgctg
     2941 gtgatttgcg tacggcttgg ctggaggagc aggacggggg aacttatcac tgttcaggct
     3001 atctgtgggg ggatattagg ccctatgcgc tggaggtaga ttttataggc ctggctcagg
     3061 aagctgccct gtgggggtgg acagaaccgg attgcgcagg agaagtgtat caggtggatg
     3121 ggagccaggt tggaccgctt tttgccccgg gtaagactgc cattgcgctg cagttgccgt
     3181 gggaggggct gattgtcgcc cttggcgaag taacgctgca aacttgggtt cctggaagca
     3241 aggtgatgct ggtggcagct ggcgaggtag agctagcagg cgtagatgaa gtatgggagg
     3301 gaagtttatt tatctatagt ggagagaaga tttattgtga tcctggcgga caactgcagt
     3361 tagcaggttg tttggtgtca ccactgttgg agttaacggg cttaaagatt acttatgatg
     3421 atcagactgt attaaaccag ctagagttag tgccggctga attgttacag gtggcaccaa
     3481 cttttgattt gcagtggctg gagttggcac cgcggcgatg aagaagcatg gtttttcttt
     3541 gctggagttg gtgatggtag tggtgatcct gggtatttta ttgggggcag ctgtgccggc
     3601 aataagcaat ctgggccagt ggccgttgaa aacagcagcg caggaattag cagcggcgat
     3661 gcgggaagca cgtgaaacgg cgattgccga agggcagact tgcacgctgg tcttttatga
     3721 tatcggcgga cgataccgcc ttgatctgcc ggcagggaca atctggacga cgctgccaga
     3781 ggagttacag atgattgcca actttcccgc tgtagataat cgtgctaccc tttattatcg
     3841 ttatacaggg gcgcctaacc gcgggggata tgtgcgtctg caggatcagg caggcagacg
     3901 cttgtatgtg attgtgacgc cggtgaccgg acgggtacgc cttgatagtg ctcctcccta
     3961 ggaataaatc acgaagtagg caggacatgg taaaactatc gcgaattata gtatggcaat
     4021 gccgcagggt attgccatct tgcttgtggt ttagtaaaat ctatttgcta cagccttatg
     4081 gtttgtgtat actgaggtaa atatgaggca gcagggggtt tgccatgaga agtattgccg
     4141 gaaaaaacat agtgctgatt ggctttatgg gtacaggaaa aacagaagtg ggcaaactgt
     4201 tggctgcttc cttaaaccga cgtttagtgg atacagatca aaccattgtg gaaacagaag
     4261 gtcgccccat tacggagatc tttgctcaag agggtgagcg ttactttcgc cgcctagaaa
     4321 cagcagtaat cgagcagctt tgtcagcaaa gaggactggt catccccact ggcggtggtg
     4381 caattttaga tcctcacaat gtagctttgc tgcaggccag cggtctggtg atttggcttg
     4441 atgccagtgt cgcagaacta gaacgtcgtt tggcccatga ccaatcccgg cctttgctga
     4501 caggtgagac cggtgtgggc gaactttacc agcaacgaca agctgcttat cggcaggctg
     4561 cccatgagcg tgtcgatacg acagctaaac cacctttagc tgtggtggca gaaattatgg
     4621 ccatcatccg taaaaactga gagaaatact ggagaataac tggttaggag aaggattctt
     4681 ttaccgttaa agcgaaatac tatcagataa aagagcacga gcctgtacaa tcgcggcagg
     4741 ttaaagatgc gacaatttgg tggttaggga gagataacaa tggacaggct gaatgtgttg
     4801 cgtaaacagt tgcagctaga gggcagagag gctattttgg ttgccaaccc gattaatgtg
     4861 gcttatctaa gcggctttac tggtacagct ggcagtctct tgattacaaa cagccaagct
     4921 tatctgctga cagacttccg ttatgtggag caggctcagc agcaggcacc agctttcgag
     4981 acgatagaca cgaaaaacaa gctttggccg ttggcagctg acttgttggc cgacaacgcc
     5041 tgccaaacgc tgacagtaga agcagattat ctgacggtag atacatatga agaagttgtg
     5101 acaggtttag cggcaattgc agtagaggcg gcagcctcgc cagtaacccc catccgtgtt
     5161 gttaagacgg cagaagaaat taaggcgatg gaaaaggccc aaaagttaac ggatgccgcc
     5221 ttcagtcaga ttttagaaca gattaaacct ggtgtgcggg aaagagagct tgccctagaa
     5281 ctcgagttta tgatgcgcag gggcggtgcc agcggtttgt cttttacgat gattgtcggc
     5341 tctggcagcc gttcagcttt gccccacggg gtggcatctg aaaagatttt agcggtggga
     5401 gatgcagtag tgcttgacta tggctgcatt gtggatggct attgttctga tatgacgcgg
     5461 acagtttttg tcgggcaggc cagtgacaaa caacgtgaag tttaccagct tgttttagca
     5521 gcgcaagaag cggcgctagc aaaactgcag gcaggcgtga gcggcagaga cggcgatgct
     5581 tttgcccgtg aagtgatcac agcagcgggg tacggagagt acttcggtca tggcttaggc
     5641 catggtgtag gacgcgaaat tcatgagtca ccacgtttat cagccagtta tgaagggctg
     5701 ctggaacccg gcatgattgt tacggtggag ccggggattt atctgcctgg tgagtttggt
     5761 gtacgcattg aagatatggt ggttattgaa gagcagggga ttcgaaattt aacagcgagt
     5821 tccaaggaat tatattgctt ataagatgga ggggacttga tgatttcaac cagtgatttt
     5881 cggacaggct taacgataga actagacggc gttgtgtttg ttattgttga ctttcaacat
     5941 gtcaaaccag gtaaaggagc tgcttttgtc cggacaaaat taaagaatgc caaaactggt
     6001 tctaccactg agcgcacctt ccgggccggt gaaaagatgg aaagggcgat tatggatcgc
     6061 cgcgcaatgc aatatcttta taattcgggt gatgagtacc atgtgatgga tacagaaaca
     6121 tatgaacaac ttgctctgac tggtgagcag ttgggcgatg gtctgaagta tctaaaggaa
     6181 aatatggaaa tcagtgtact cttttatcag gaacaagttt tcggtgttga ggtgcccaat
     6241 tttgtggaat tagaagtagc ggaaacagaa ccaggcatta aaggtgatac tgcttccggt
     6301 ggttcaaaac cggcgacgat ggagacgggt ctggtagtac aagttccctt ctttattaat
     6361 gccggtgatg tgttgcggat agacacccgt tcgggtgagt acatggaaag ggtataaagc
     6421 ccattacaat tgaaggagga tactaatccg atggatgatc ttacatcgcg tgtggcgtat
     6481 ttgaaaggac tggcagcagg actaggcttt gatccaggga gccgtgaagg aaaactgttt
     6541 acacaactga ttgatgtcat tgacgcttta gctgaatcag taagtgaact gcaggaagat
     6601 tatgttgata ttgtagagta cgccgaagct attgatgaag accttaatga tttggaagaa
     6661 gatttctacg atgaagatga gctgttggac gacgatgagg actttgacga tgaagagttt
     6721 gatgaggatg atatgttttc ggtagaatgc ccggaatgcc atgaaatcgt ctacattgat
     6781 gatgacatgt tagaagatga tgatgtggta gaaatcctct gccctaactg tgagcgtgtt
     6841 gtttttgtca atgatgatcg ggatttcgat gacttccaag accttgaagt tttagatgaa
     6901 gagggtcagg aataacctgg agtcaggttt aacttaaaaa acatacttaa aaagccgttt
     6961 cacctaattg gtgaaacggt tttttttggg tttgccctgc atgggcgaag gcagcgtttg
     7021 atgacttcaa gtaaataatt caagcctgac cccaggtata caagcctgac cccaggtata
     7081 ttagtgagcc tgtcctcata gtaatgtagg ggtgaaggca atgaaagatg aaaattggca
     7141 agagcagatt ttaccacttt taccgccgca gctgcgagct atttttacga gcctgccagc
     7201 tgccttggca gctagcctgg aggaaattcg gctgcgggct ggacaacctt tgatggtgca
     7261 gagcgggcga ggtgaagtgt ggctggggct agctggtcct gtgacggcct taggcgagag
     7321 ctgccggatt acggcagctg atttgcagca cattttgctg cagatgactg aatactcgct
     7381 ttatgcgcgg gatgaggaat taaaaagggg ctacttggcg ctgccaggcg gccaccgtgc
     7441 cggttttgcg gggcgggtgg tgatggaagg taaccaagtt aaattgctgc gcgatattaa
     7501 tgcgctgaat attcgcattg cacggcaatt taagggggct gccttaaaaa tactgcccca
     7561 tgtctactgt gacaagacgc aaagagtacg gcatacttta ctagtttcgg caccgcaggc
     7621 ggggaaaacc actatgctgc gtgatttagc caggctgctg gccaatggtg atcaacaaaa
     7681 gggacggccg ggttttaaag tggggatagt ggacgagcgt tcggaattag ccggttgttt
     7741 tagaggtgag ccgcaattag atgttggtgt acgcagcgat attttagatg gctgtaccaa
     7801 ggctgagggc atgatgatgc tggtccgttc catgtcaccg caggtgattg tcacagatga
     7861 gctgggcaga gctgaagatg ctcgcgcggt ggaagaagcg gtgaatacgg gcgcaagtat
     7921 tttggccaca gcgcacggac aaaatgcaag agaactcata agaaggccga gcttagcata
     7981 tctattggag aacggattat ttgagcgggt gatagtgttg tcccgacgcc atgggccggg
     8041 tactgtggaa gatatttaca cgggacattc tttgcaagag tggttacgtg ccgggaggga
     8101 gtgcaatgtg gattaaatgg ctgggggccg ttttgattct ggcggcagcc ctgatgtggg
     8161 gcaatttaca ggctgccaaa ttacgtgccc gcgtgcgcga actggaagag ttcagactgg
     8221 ccttacgctt attggcagca gaaattggct acacggctac acccttgccg cgcgctttag
     8281 agcaagttgg taaacgtctc ggcaaccagg gggtgcaggc tttcttggcg gcggtccgcc
     8341 agcagctgca ggatccgcag actgctgatg cttcaactgc ctggttggct gccgtgcaca
     8401 gtgtcaagcc gctgctgtgc ttaacaccag aggattgggc ggtggtggca cgggcctcgg
     8461 caggcttagg cggcttggga cgagatgacc agctgaaaca attggcggcc gcggaaacgc
     8521 agcttgctgc ccaggcggtt gaggccgcgg cactgtgtgc tagtaatgag aagatgtggc
     8581 gttatttagg agcgttgtcg ggcttaacgg tcgtaattct gcttctctaa aaatctctgg
     8641 tgttggaagg agaaagttgc atgaatggat ttaatgtaga tcttatcttt cgtattgctg
     8701 gcattggcat atttatttca gtacttaata ttgttttaaa gcaggcggga aaagaggagc
     8761 aggcccaaat gctgacccta gggggcgtag tagtagtgct gttgatggtc atccaactca
     8821 tcaatgaact cttctctagc atcaaaacaa tttttaacct ttattagatt gacagtggta
     8881 agggacacgt caaagctgaa agggagaggc cattggaaat cattcagatc gttgcttttg
     8941 cactgatagc tacattcatg gtggtggtgg tgcgggaaac aaaaccggtc tatgccatgc
     9001 tgattgccgt ggccgctggg gtaattatct tcctgcgtgt ggccgtctac ctgtccacag
     9061 tgctccaaat gttattggat atgactttgc gggctaatat ttccttggtt tatttaaata
     9121 cattgcttaa aatcctgggc attgcttatc tggcagagtt tggcgcgcaa atctgtcgtg
     9181 atgccggtga aagtattatt gccggcaagg tggaatttgc cggcaaaatg ttaattttgg
     9241 taatggccat gccactgtta gtggcggtgt tggagacaat tattaaattt gtaccgtagg
     9301 tggcatatga aaaaaattgg cacggttatt ttactaactt tatgcttatg gctgaccacc
     9361 agtccggtgc tggcaaaacc agcaactgaa ctggtacagc agcaggcaca agagctggag
     9421 atgacagaaa ttgaatcttt ctgggaggaa ctgcagcggg agtctggtga atatttgccg
     9481 gactttacct ggcacaacat tcttgagtgg ttccgcccgg gagagggaca aggcttgtca
     9541 ttttcagaga tcatcaatgg tttatggcgc tttatgtggc gggagattta tttcaattta
     9601 aatcttttgg ccaagttact ttttctggcc gtaatcgcgg cgctgttgaa gaacttggaa
     9661 aaagcgtttg ccaatgatag tatctctgcc cttacccagg cggtggttta tttagcactg
     9721 gtttccattg cgatgcaaag ttttgccgtg gcgataggca ttggccggga tagcgtcaat
     9781 aacatggtgg aattaattct ggccgtgatc cccctcttgt tagcattact ggcgtcttta
     9841 ggcagttttg cctctgcggc tctctttcgc cccatcatta tttttgcggt caactttttt
     9901 gcggccttga cgcgggatgt cgtcttcccc ttgatttttc tcaacaccat tctctctatt
     9961 gttaattatt tttcgccccg tgtgactgtc agtaagctgg ctgacttact gaagaatagt
    10021 gcagtttgga cgatggggct ctgcatgacg atctttactg gtttgatgtc catctatggg
    10081 gtggcttcca cggtcggtga tgctgtcagt ctgcggacgg ctaaattttt aactagttct
    10141 ttcttgcctg tggttggcgg tatgatttca gatgctgtgg aaacggtggc cggcgctact
    10201 ttaattttaa aaagtacagt gcatatcgcc ggcatcctgg tgctctttta tctcgtagct
    10261 tttcccctga ttaaaatttt ggtgctagtc tttatttaca aattggcggc tgctttaatt
    10321 cagccgttgg gggagaccag cctctgtgat tcgctgaata cattaggaaa ttgtttggct
    10381 ttagtttttg ctgctgtggc tgtggtgagt gtgattttct attttgcgct tactgtgatc
    10441 tccggcgctg gtaatgcttc gtttatgtta aggtaggtga gactgtgcta gctgttttat
    10501 cagaagtggt gcgtaatatt gtgatcttga ttgttttagt gacgatcttg gagatgatgc
    10561 tgccccgcaa agagtttcga ccatttgtaa atatggtggt aggtttggtg ttgatgctga
    10621 tgctcttggc acccttgcgt tcacttttgc agctgccagg cttgctagac ccggtggtgg
    10681 aaatgcgtga tgccttgaca gaagaaacta tcgccagaca gcaggcagag attgagcaaa
    10741 tgaattggga tttgacgctg gtacggtatc ggcagctggt cgaagaaaaa gtggcgcagc
    10801 tcttgcggga ggctgattta gagattgtca gcatggacct gcaggtggag gaggatgtta
    10861 atcatttaga gtttggtgtg ccgcggcaaa ttagcgtggt ggcacagccg ctgcagccgc
    10921 cagcggggtt gattgagcca gttgagccaa ttgaggtagg gattggggag ggggaactgg
    10981 tgcccggggg cggtgtatat gaagcagagt ttgctgccca gatcgcggca gcactaggaa
    11041 tttcggcgga gcttgtgcaa gtgcgggtaa tttatcactg agggggtgtg tgtagtgagt
    11101 gaacaatttt ggcagcgcgt attagataaa gtgcagggca ggggggacaa ggacggcaaa
    11161 aaacagtatt ctacctggtt cattgtggca ttagtggctg tcggcattta ttttatgtac
    11221 attagtgcca tgccggataa aagttcaggc gagcagccgc tgattcagcc gggtggaggc
    11281 gcggcagtaa ctagctttaa aagtgattat aaagatcagg tagagcggca gttaagtgca
    11341 gaattacaaa gtgttaaggg tgtagacgct gtgcgggtct tcgtcaccct agccagtggg
    11401 tcagaatatg aatatattca caatcaggaa accactaacc gcaagactga agaagaggat
    11461 agcggcggcg gcaaccggat tattgaagaa gaaaccacca aaacacaagc tgtcatgcag
    11521 cagacaggct ccggtcaaca ggcagtagtt gtgcgagaaa agacaccgga gattgccggc
    11581 gtgttagtaa cagttaaagg tgatggcagt gctcggttat tagaagatgt gacgctcgcg
    11641 gtcagagcgg ccctatattt acccgcgcat cgggttaaag tattggaaat ggagtaggag
    11701 gaggggaagg catggagagg gaaacttcaa gaaaagtaac gacaattgtc ttgttggtgt
    11761 tggccttggc ggcggtgtgg tatgctaaac aatttcagaa tgaccggata gatattgttg
    11821 atccagcaaa gcagtcagaa acggaagatc cgcttggtca gggggagcca gcagctgagg
    11881 aagaaccagg aacgggtgaa ggggagaatg aaggcgatag tgagggtgac agtgaagaga
    11941 cgacagcgcc cgtggtcacc gctccggaaa gcatgggttt agaaagttat gaagcatatt
    12001 ttgtggagta ccgcctgcag cgcgaccgta ttcgtgcaag tgaagtagaa atgttcaaca
    12061 ccatgattga gaatcccaat gttagtgccg aggcgaagaa agaagcggaa gcgcagctct
    12121 taagcttgat agaacggatg gaaaaggaac taatggtgga gaacatgtta aaggcgcaag
    12181 gctttaaaga tgcagtcttt ttcattaaag acggcaaagc ccatgcggtg gtaaaagcag
    12241 ctaaactaga tcaaacacaa tttatgcaaa tcgccgaagt agtcaacagt gtgactggtc
    12301 tcagcatgga tgatatctct gtggtggagc atggcggctc ataacaggaa aattgctgct
    12361 aacatggaat tgtattgcac aagaatcttt ggtataatat tcaatattac agcatggctg
    12421 taatcaaggg aggtgtattt tttgtcagcg gaggaagaat tcataccgac agaactggga
    12481 acaattaaaa ttgccgatga cgtcgtggcg attattgccg gactggcagc tactgaggtc
    12541 gagggcgtcg ctggcatgag tggtggcttg gctggcggta ttgccgacat gctgggcaaa
    12601 cgtaatcttg ctaaaggtgt taaagtagaa gttagcaatc aacaggccct tatcgatata
    12661 ttcatcattg ttaagtttgg tgctcgcatt cctgatgtgg cctggaagat ccaagaaagt
    12721 gtaaaaaaag ctattgaatc aatgacggga ttaaaaacgg caagtgtgaa tgtgcatgtg
    12781 cagggtgtaa gttttgatac ggctaaaggg gaagagcaag aggaagatct gcaaatccgt
    12841 taagctactt aagttaagcc cctgtttgtg caggggctta acgtcataca gactactgca
    12901 tagcgcgaaa ggagatgtgg aaacctatga atacgcgcga aagactatat ttagtgatct
    12961 ttgctgtact cctcagcgct atggcggtgc tggtgggggg ggctgccctt tcactttatc
    13021 cgctggaagt tttgcaggat acagtggggg cagtttatgg tcatttagag tatgccattc
    13081 tggccgtgct gctgctggcc ttagccgtct gggcattttt aaccagtgtg cgccgcaaga
    13141 acactgtaga atcattagcg cagcctggac cacttggtga agtgagtatt agctttaaag
    13201 cgattgagaa tttagtgctt aaagctaccc gtaatgttaa tggggtcaga gatatcaaaa
    13261 cacgcatcgt ttacaccgag tcgggtttag tcatctactt gcgggccact accttgccgg
    13321 atgaaaagat tcctgagctg acagcaggac tgcaagcagc tgtcaaggaa tatgtggaaa
    13381 gctccaccgg cagcacagtg gcagaaatca aggttttgat tgagaatgtg gtgactgaca
    13441 cgattaaggc tacccgttag gaaagaaggt gtaaacgtgc ctgcgaaaac attgcggcaa
    13501 tttattgttg aacactgggg gaaaatactc ggtggcgccc tgggactgat tgtaggttta
    13561 tgtattctat tattgggctt ttggcgtagc gtactgctat ttttatgtat cgctgtaggt
    13621 gtttacctgg gacgtttatt tgatcgtcat gaagggatcc aaggaatttt acagcgcata
    13681 tggcctgata acgactgact ggagtgtgct tatgagccga cgtgcggcaa gagaagttgc
    13741 ctttaaaaca ttatttcaat atgaacaagg taaaaatagt attgaaccta gtctgtcaga
    13801 gctgctgacc gagagcggtt taaatcctca gtcggcactc tttgccagag agctggtcaa
    13861 cggtacggtg gacaagcagg aggaaattga ccagacgatt agcccgtatt tacagaactg
    13921 gcaattggaa cgcctagcgg ctgttgaccg tagtgtcctg cgcttggcag ctttcgaatt
    13981 gttatatcgt gaagatattc cccctgccgt ttctattaac gaagcactag agttatgtaa
    14041 agcttacagc ggtgaagagt cggccaagtt tcttaatgga gttttggaca agctggccca
    14101 cactaaaggc ggcagcaagg aggaaaacta gtgtttttag gcctcgatac atcatgttat
    14161 actacgtccc tggctgtcat tgacacgcag ggacgtttgc tttctgaagc acgacagctg
    14221 ctaaaagtgc cccagggtga gcgaggcttg cgccaagcaa atggggtctg gcagcacctg
    14281 cagaacttac cacaactggc gcaacagctc cagacagaga taggtcctct gcggctgcag
    14341 gcggtggcgg cttccagcca gccgcggcca gtggctggct cctatatgcc tgtctttatg
    14401 gctggccttt ccttggcccg gagtctggcg gcgctggctg gagtaccgtg tatagagctt
    14461 tctcatcagg aggggcatct ctgggcaggg ctgtggtcgg ccaaggtccg ctgggatgat
    14521 ttttatgcgc tgcatgtttc cggtggtacg acggagttgt tgtctgtcaa gcaggctgaa
    14581 cgcctgcaga ttgtggagct gggtggttca gctgacctcc aggccggcca atttattgac
    14641 cgtgtcggcg tggctatggg gttttccttc cccgctggtc cgcagttaga ggccttagcc
    14701 caaacggccg gtggggagat tgcccaggta ccggtgtcgg tacaggggtt taatatgtct
    14761 ttttcgggtc cggaaagtca tgtacagcgg ctgctggcgg gcggctctgt caaccaggca
    14821 gcagtggcgc gtggggtaga actttgtatt gctgaatcac tgcggcgggt catcattaat
    14881 gcccgtcagc agcagggcga aaaacccgta cttttggtcg gcggggtcat ggccaacaaa
    14941 tttatccgca catttttagc agaacaactg gatggtcact gtgctttcgc tcagccccgc
    15001 tttgccgggg ataatgctgt cggggcggca ctttatgcgc agcaggccgg caagaagatt
    15061 aagcttgcta acaggaaaag gtaaagagca tcacgaatat aaacaaaata ccagtgagaa
    15121 attcaagcag gtaataaata atagagtagt ggagtgttgt aagttagatg gccagccaaa
    15181 acaaacgtat ttttactgtc ggacagttaa ctcaatatct gaaaaatatc tttgccgctg
    15241 acccccagct taatcggctg cgagtaagcg gcgaaatttc taattttaag caccatagtt
    15301 ccgggcatat gtatttcact ttaaaggatg cgcaggcagc ggtgcgttgt gtgatgtttc
    15361 gcagtgctaa tgcccgtctt actttcccac cggcaaatgg tgtgaaggta gtagccagcg
    15421 gcaagttgtc catctatgaa cgtgatggcc aataccagct ttatgtggat agtctgctgc
    15481 cggaaggtgt aggcagtctg tttgccgcgt ttgaagcatt gaaagctaaa ttagctgcgg
    15541 aggggctctt tgaagccgct ggcaagcagg aattgccgcg ctttccgcag cgcatcgggg
    15601 taattacttc gccgaccggg gcggcgatac gtgatattgt caccactata acacgtcgtt
    15661 tcccgccggc tgaaatactg attgtaccgg tgctggtcca agggccggag gcgccgcggc
    15721 agattgcgga agctattgat tttctcaata aggtgccggc actggacgtg atcattaccg
    15781 gacggggtgg cggttctatt gaagagttat gggcctttaa cgaagaaatt gtggcgcggg
    15841 ctatctttgc ctcgcagata ccggtgattt cagcagtggg gcatgaaact gattttacga
    15901 tcgccgattt tgtggcggat gtgcgggcgg ctactcctac ggcagcggca gaattagcgg
    15961 tgccagaaca gcgggagctg cagcagtttt tagaaaccag ccaaaaccgt ctgctgctaa
    16021 agctgcgtac caaactaggg caagaaaggc gttacctgga acagctggct gcttcacggg
    16081 tgctaaccag acctttggag agggtagagc aggccaaaca aacgcttgat aatctgtctt
    16141 cgcgcctaga aacacaaatg gtctaccatc tgcagaatca gacagccaaa cttcaattgc
    16201 tgtcagggaa attaggggcc ttaaatccgg aggctgtgct ggaaagaggg tttgctattt
    16261 gttatgatca agcgggacaa gttgtgcggg cggcagacca actgcagaca ggcgacgcgg
    16321 tagaaattcg cttgcggcga ggtagagcat cggcggaaat attgcagaca aaaacggagg
    16381 aagaaaatga gtaaaaaaac aaccccgata gcgggtttaa actttgaaga agcactggct
    16441 aaattagaac aggtgctgca gcaattggag cgcagtgact gtcctttaga agaggcgctg
    16501 gcactatttc aggaaggtat gcagctggtg cagcactgtc gtactaaact taatcatgcg
    16561 gaagataaaa tttctattct cttaaaagaa gcagaggagt ttgtgccctt taacggcgaa
    16621 ggtggcagcc atgaccttta agccggcaga agttattgtg gaggttaacg aggccttgca
    16681 gcaattctta ccgcccccca ctactttccc cacagagata catcgtgcgg tgcattatag
    16741 tgtttttgcg ggggggaaaa gattgcggcc gctgttaact ttagccgccg cagatatctt
    16801 taccgtgccg cgtcagcacg ctttacctgc cgcttgtgcc atagaaatga ttcataccta
    16861 ttcgctcatt catgatgatt tgccggccat ggataatgat gattatcgcc gaggccgccc
    16921 ctcgaatcat aaagtatttg gtgaagggat ggcgatcttg gccggtgatg ctttactaac
    16981 cttggctttt gaaactttag ctaccaaagc aagcgcttac tttcctgcgg aaactgtggt
    17041 ggctctgcag caggagctca gtcaagcgtc agggatggcg gggatgattg gcggacaagt
    17101 agtagatatc gtctctgaag ggcaggcggc caatgcggaa atattggact acattcatcg
    17161 tcacaaaaca ggtgctctct ttcgttgttg tctgcgctgc ggggcgctga tgggacaagc
    17221 tgccgcggcc gagctggctg cgctaactga ctacgccgaa aagatcggtt tggctttcca
    17281 aattgttgat gatattttgg atattactgg tgaagcggct gccctgggca aacaaacggg
    17341 ttcagatgcc aataaacaaa aaatgactta tccggcgctg tatggtttac agcaggcaca
    17401 agctaaagca gataccttac tggaagaagc tttgctgctt ttacaaccat tcggcgcagc
    17461 tgccggcagc ctgcaagctt taaccctgat gcttgtccaa cgccatagct gatttgtagg
    17521 ggcgaactgc gttcgcccgc acattatttt catcatcagt aggtagagtt gtggttgtaa
    17581 tagtttctgg cgcacaaagt gacaccccta cgggttgaac cagaatgagg tccaatttaa
    17641 caaaaggctg ccaaggattt taatttcatc aagcaaaaag atgacagggg cttacctttg
    17701 ccatctttaa ttttgtgttg tctccattgc tcgctgataa agtgtaataa ttttaaaaat
    17761 cgtataaatt tcttgttcac ttgcttgaca cttttggcag agatgcatta ctttctccag
    17821 ggtgtcagca tttttataat agtcggtatg ctcttccagt tctgtggtga tccgctcata
    17881 taatattttt tcctctgggg taaataaatc ttcaatgcat tctactgccg tgtggggacg
    17941 agtgatggtg ccgccttggt ccagatattt ttcctgccaa gcatggccgc tcctacattc
    18001 aatataaaca tggtgctgag tgggaatcag ataaagacct gcttcgccac atttaatgca
    18061 gattgcttgc ttctttttat ctttggataa tgccaattac tttaactccc ctaacaattt
    18121 caatcggctc aaaggcctgt tggtccaccc ataagatacc ttcttcatcg atccggatat
    18181 ccaagttcag gccagccaga ttaaatctgt ctatgtagta gggggttact aaaacaaaga
    18241 cagagttaat ttgcgtagcg ttaagttcct gggtaccggc aaggtttgcc tgattggaag
    18301 cattgatgag gaagttatat gctggggccg caccatggtc attgctcatc acaatcgact
    18361 ccttttaaaa tgctgacaag ttaagctact cttcactgtc tgtgatcttg cgatcactgt
    18421 gcatccggag atgctcgtcg ttaatggtga tgccatcgat gccttcaatg gtcagatctc
    18481 ccattttatc tatggtgatg gctaaaggta ttttttgctt ggcgagggcg ctttctctca
    18541 ggtaatccac accgatgagg acgccgacgt ttaattggta gagagtggta gtgcgtgcct
    18601 gacacatatt ggcttcattg gagagattag aaaggtagac caatttactc ttctgcatct
    18661 tgatcatcct tgagcatagt gtgtaaaatg ctttgcaatt ttgctgtttt ttcttcgtta
    18721 gtcagttcag tgttgaggcg taactgataa agttcttcta acagagggga ataagcccag
    18781 tttggtaagt ccaaacttac ttccagctcc ggcgttttaa tttttcgttc gctcattgca
    18841 gccaccttaa aatttaatag ttgtctgctc actcttattt ttatctttgc tttcactgac
    18901 agtaatggtt tcgccttttt ctgcttcttt ttgcaaagct aacatctgga gtgtttcttc
    18961 acctgtattt agtttattgt gtgcttcgat attgacggaa aggttacgat agcggggatc
    19021 atcttcttta aaaacttcac cttccagcaa aacctgccct gatttgtaga taataatctc
    19081 tggtatcttt ttatggcgta aaggcttaat ggtaatccag gggtttttca atttttatca
    19141 ccttctaggt aagattcaat ttaagcattg attcggtttg cgtaatcact gattccgtgg
    19201 agagggtcat atttgactgt tgatttgtat ttaattgatc aataatttca gcttccataa
    19261 atacatcatt ttgaaataca tcattttgac gctggcgcgc aggctgcttg ctctcttccg
    19321 tcatccctag agcctccttt gccttactgg cgtttatctc acttcacagt ttagtaaagc
    19381 tgtatacgca gcccgtttag caaccggctg ctgaaagcac agactggtaa taaagcactt
    19441 gctagctaca taatatgtgc aggcgacgag agttgttcat gggcaaagac ccctagtgca
    19501 aaaaacaagg ggtctttgcc gcggcagcta tgagttgatc gtgtcattat ctaaattagt
    19561 ggagaagccg aattgtgctt ccaggacgtt gacaatttgc gcagctacta acacgagaat
    19621 ttgctgggca ttttggttaa ctaccttttg attgacaaca ctggccaata atacgtctgg
    19681 catttctttc cctcccgctt taatcgttta tttatttggg tataatggac tgcaagactt
    19741 taacttgttc ctcacgtgta actccttcgc cccttgcgcc cgctagtttt tctagaattt
    19801 caaaagcatc aaattgcatc ggttcgtctg ttgattcaat gccataacca tttacggaaa
    19861 aatcaagggg gttgaagggc tttgaatcaa gcgttaaacc ttttctcgtg aagccgattt
    19921 tcaaggtttt cgctcctttc tctttagagt aaattatcta tgccgccttg gacaacgtct
    19981 tgatcttcat tagtggcgaa agcaaattga gcttcagtaa tattaagcac ctgggcggcg
    20041 actaaaacta ggatttgctg tgcattttgg ttgatagcct tttggttaat cacacttgcc
    20101 gcaaaaaaat ctacagcgcc aaattgtctg acagtttttt gcgggatggt gccggtggca
    20161 tactgataag caaatttttt catctacata ctcctttcta gcactatcaa gtactaaata
    20221 gccaagccct caatggtgtc actgtcatca ttggttgcga aaccaaattg agcctcaatt
    20281 agatttgcaa cttgcaccgc aacaagaact aaaatctgtt gtgtgttttg gttaactgct
    20341 ttttgattaa tgacactagc gctaaaaaaa tcaccagtaa attgtctaga catgcgctca
    20401 cctcctgtat tgctacatag tatgaaaggg tgtaaagcag tgatactatc agagacagca
    20461 tacttaatat atggataaat tattatacaa tatgatacac agcaggctat catttggtga
    20521 caggcacaga caagaaagat atccgcgcca cgccaggcta aggatgctta atcggcagat
    20581 aagggcaaaa tgagccgcag aggtaaatgc gctaattgcc ctaaatagcc tcatttaggt
    20641 ctaacgtggg ctgccaagcg agttgtagct ttaaaggtgg tatgctacaa tagacccaac
    20701 cagtaggtga cgcagtattc tagttgatgc ggcactttcg aagacgggcc taaaaatccg
    20761 tatagggcat atcgatgaag tttctggtgc tggctgccga cgcccagttg ggggctggtg
    20821 ctgggagtta aggggttggg gcgatctgca aaggcatgca ggcgtagacc ctagcctcgt
    20881 ggagacccaa gtatgtggtg gattaccgta gtaggtaaag gattacggct tgggaagaac
    20941 ctgtatgcgg ttcgcgctgt gtacagtgta gcctgccttg agtggtgacg gtggatgctt
    21001 aaaattatgg aggaaaccgt tgttgcaaaa gaggatagaa agtgggcacc gtgttataga
    21061 ggaaaactcc tagactgttc taacagggaa agtcgttagg gattaaagta cggactcagt
    21121 ggcaatccag tctcattgtt ggcgacacaa tgaaattgat tataaaggga aaccgcctag
    21181 tacggcgacg actaggtaat taattgggaa aacctgctgg acctcaagcc gtaacgttta
    21241 tctaacgggt tgctcaccta cggttctatg gtatataatt ggcataatta ggccttgggc
    21301 ctgctataaa gagtttgttc acaaactctt ttttcctatt gtaaaaacta aacaaaatgt
    21361 gtctttaact gttcgctgtg atgagcagca tctttgtacc aatcatagtt tggcagtcgc
    21421 tattgctttc taaataagca tagtctgcag ctgataaaat cgaggtggga gaagttgggg
    21481 caaaactcac aaccaggtaa gcagctaggc tgggttgtta cgatcacaat ctggacattt
    21541 ttgctttctg tgctgttaag tttcgtctcg cagtccgttt ttctcggtct gcgttcaagt
    21601 attatcgctt tagcgatact tttaattatt atca
//
LOCUS       DASRMS010000228         5132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_803646, whole genome shotgun sequence.
ACCESSION   DASRMS010000228 DASRMS010000000
VERSION     DASRMS010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 5132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5132
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_803646"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..85
                     /locus_tag="VFC74_10900"
     rRNA            <1..85
                     /locus_tag="VFC74_10900"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            169..285
                     /gene="rrf"
                     /locus_tag="VFC74_10905"
     rRNA            169..285
                     /gene="rrf"
                     /locus_tag="VFC74_10905"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            858..2330
                     /locus_tag="VFC74_10910"
     CDS             858..2330
                     /locus_tag="VFC74_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024240.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HZK25874.1"
                     /translation="MESNVYVETPNNLPIYDECDILVVGGGCAGHSAAIAAARAGAKN
                     IILMERYGYMGGDVTGGYVIMVPKLSWYGKSFVRGLQEEWFTRLEKIPNAVLGPKLNE
                     IGMTDPAMINAWKAIHGCVSESTPQRLVRAVYYEPNQLKIEMDKMLLEEKDKIRVQYH
                     SWGTKPIMEGNEVKGVIFESKEGRKAIFAKTVIDATGDGDIFRQTGTPFRALADSNTR
                     SSTTALVWRIGGINWDLYYEWTKVNPEANKALRAGLARVAGYRCMPIPSNQNDVCWIN
                     NWHANKDCANIKDATHTEIQTRDTMRDVLEYLRVTCPVAFRNAYLYDIAPQLGTRCSY
                     RLQGEYIMTPNDFAFAPKFDDVIAWHSTICMINDCAPIEIPYRAILPQQIDNLLCPGR
                     HISADDVAIDWLTLIPQCIGTGQAAGVAAAVALADGSNVRNVNIKKVQDILVEQDVPL
                     PRNDKTDPSYTACCEEHEYGLYTDLAKKAKAEGIVEYRQW"
     gene            complement(2535..3914)
                     /locus_tag="VFC74_10915"
     CDS             complement(2535..3914)
                     /locus_tag="VFC74_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25875.1"
                     /translation="MSFFKVFMLILFYPILPIMYFVLINEAKPRKNIILGVTLPRLAL
                     NDSDLIDICNQFKYALRKFVLLLAIPPFAVFCVDRSSIMMLILMYWLIFAIIVPFVPY
                     TRYHRKLKALKQSKNWKVGTTQKILVDIKAAAEVENQTNAWLFIPAIIISAYPVVYEL
                     TAGREQTHISIFLGCCIMLFTTVLFYIFYRLASQLRTEIIDEDSKYNIALTRVRRYNW
                     ARFWIWAAWLNTFYSIIFWLGINGRTSFTVFMLSTIIYAAAMLWAVLKAEFTTRSMQH
                     KLAAESTKPIYMDEDSHWINGMFYYNPQDQKTMISNRVGIGMTMNMARPAGKIVIAFS
                     LLCLLIIPFLPIWMIAEEFTPLKVEKRDSAIVITHLSEIATIDTAAIESIELLEELPT
                     SRKIAGTGLENLKKGTFDVEGYGRGKLYLDPQQPPFILINTSHGFIIINMHDEICDFV
                     KTKFPLTGA"
     gene            complement(3911..4291)
                     /locus_tag="VFC74_10920"
     CDS             complement(3911..4291)
                     /locus_tag="VFC74_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HZK25876.1"
                     /translation="MILALDFSSETPIYLQIRNQILLAIAEGTLNHGDKLPTIRALAN
                     ESGINAMTVNKAYQLLKQEGIISIDRRSGTKVSLPYDSKAETLAKLTKELKLLLSEAK
                     VKGISETEMLELFNRISKEMEGTR"
     gene            5070..>5132
                     /locus_tag="VFC74_10925"
     rRNA            5070..>5132
                     /locus_tag="VFC74_10925"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1455 a         1105 c         1175 g         1397 t
ORIGIN      
        1 aaagaagatc tggttgatag gccggaggtg taatggcagc aatgtcttaa gctgaccggt
       61 actaataggt cgaggacttg acctaaaaat atctatgtga aattgcgaca ggggacggtt
      121 ctcattgtcg catgctctga aaatcgctgt tcagttttga gagaactttc tggtggaaat
      181 ggcggagggg ttccacccgt tcccattccg aacacggccg ttaagccctc cagcgccgat
      241 ggtacttggg acgcgagtcc ccgggagagt aggtctctgc cagatccctt tttctacgac
      301 gcactgagta atcggtgtgt cgtttgtttt atcccgcacg attgtctccc ggtgacatgt
      361 cccctccttg ggcgaatgct acataaccag gtgaccacgc tattggaggt ggtagctcat
      421 tgtatactct ttagcgtaac ttgtatcctg ataactccgt tcgctttgct cactgggtta
      481 ggcctgagta ggtctctgcc agatttaact ttgaacgacg cactaactta tgttggtgcg
      541 ttgtttcttt atgtgcccgc actgttacct cccaatgtgg gagatgaaat tacttgatcg
      601 gctgcataat gtgaaaatat gctaaaaata atgaattgcc ttacaatggc accagtgcta
      661 taatatggtt attaactcct agctattatg cttgtgttct tggacgatca tgctctccaa
      721 taatgtcggt gcgtgatctg gagttatccc tacttctatg tgaagcgcct gcatttaacc
      781 tcgcttggcg cttaatagct ataaagtact ttgataaagt acaaaagtaa aaaacattaa
      841 atgaacggag gaatatgatg gaaagcaatg tgtatgtgga aacccccaat aacctgccca
      901 tctatgatga gtgcgatatc cttgtagttg gtggcggttg tgccggacac agcgcagcca
      961 tcgcagcagc gcgtgcgggt gcgaagaata tcatcctgat ggaacgctat ggctatatgg
     1021 gcggtgatgt tacaggcggt tatgtcatca tggtgccgaa gctatcctgg tatggtaagt
     1081 cctttgtccg cggcttgcag gaggaatggt tcacgcgtct agagaaaatc cccaatgccg
     1141 tgttaggacc taagctcaac gagattggca tgaccgaccc ggccatgatt aacgcatgga
     1201 aggccattca cgggtgtgta agcgaaagca cgccccaaag acttgtccgt gcagtctatt
     1261 acgaaccaaa tcagctgaaa atcgaaatgg ataagatgct gctggaagaa aaggataaga
     1321 tacgagtgca gtatcactcg tggggaacaa agcccattat ggaaggcaat gaagtcaaag
     1381 gagtcatttt cgaaagcaag gaagggcgca aagcaatttt cgccaagaca gtgattgacg
     1441 ccacggggga cggcgacatt tttcgccaga caggaacgcc gtttagggcg ctggcagaca
     1501 gcaatacccg ttcaagtacg acagcacttg tctggcgtat cggcggtatc aactgggatc
     1561 tctattatga atggacgaaa gtcaatcctg aagcaaacaa ggcactgcgt gcagggctcg
     1621 ccagagtcgc tgggtaccgt tgcatgccga ttccctccaa tcagaatgat gtctgctgga
     1681 tcaataactg gcacgcgaat aaagattgtg ccaacattaa agatgccaca catacggaaa
     1741 tccagacacg cgacacaatg cgggatgtac tcgagtattt gcgcgtaacc tgcccagttg
     1801 ctttcagaaa tgcctatctt tatgatatag ctcctcagct cggcacacgt tgcagctaca
     1861 gacttcaggg cgaatacatt atgacgccga acgatttcgc ctttgcgccc aaatttgatg
     1921 atgtgattgc ctggcattcc acaatctgca tgattaatga ttgcgcaccc attgaaatcc
     1981 cgtatcgggc gattctgccg cagcaaatcg ataatttgct ctgcccgggc cgccatattt
     2041 ctgcggacga cgtggctatt gactggctca cactgattcc tcagtgcatc ggcactggac
     2101 aggcggccgg agtagccgcc gcagttgcat tagcagacgg ttcgaatgtc aggaatgtga
     2161 acattaaaaa agtccaagat atcttagtgg aacaggatgt tccgctgccg cgcaatgaca
     2221 aaacagaccc atcgtatacg gcctgttgtg aagaacatga atatggcctc tacacagatc
     2281 tggccaaaaa agccaaagcg gaaggaatcg ttgaataccg ccaatggtaa agctgttcag
     2341 cttgtaagag cctgcgcacc acggtgacgg gggacggttt ttttcacacc tgtatttagc
     2401 aaagtttcgc ccttggttgt gcatgctaat acctggtatc ctgataaata cactcgcttt
     2461 gcttactgtg ttagcccagt gtatttatca tcaccatatt ttaacgacgc actaatcaat
     2521 aagtgcgtcg ttttttatgc gcccgtcaga gggaacttcg tctttacaaa gtcacaaatt
     2581 tcatcatgca tattaataat aatgaagccg tgactagtat taattaaaat aaagggaggc
     2641 tgctgtggat ctaaatataa cttacccctg ccatagccct ctacatcaaa agttcctttc
     2701 ttcaggttct ccaatccagt acctgcgatt tttcttgagg ttggcagctc ttccaaaagt
     2761 tcaatagatt ctattgctgc tgtatcgatt gtagctattt ctgataaatg agttattaca
     2821 attgctgaat cccttttctc aactttaagc ggagtaaact cttcagctat catccaaata
     2881 ggcagaaagg gtattatcag gagacatagt aatgaaaaag caattacaat tttgcctgcc
     2941 ggtcttgcca tattcatagt cataccaata ccaacacggt tgctgatcat cgttttctga
     3001 tcttgcgggt tgtagtaaaa catgccgtta atccaatggc tgtcttcgtc catatagatg
     3061 ggtttcgtgc tctctgctgc taatttatgc tgcatgctgc gtgtggtaaa ttccgctttt
     3121 aacacagccc acagcatagc tgcagcataa atgatcgtag aaagcataaa gaccgtaaat
     3181 gaagtccgac cgttgatccc taaccaaaag atgatactgt aaaaagtgtt tagccaagct
     3241 gcccagatcc aaaagcgtgc ccagttatat ctcctaaccc ttgttaatgc tatattatac
     3301 ttactatctt catcgattat ttccgttcgt agttgtgaag caagccggta aaatatatag
     3361 aataaaaccg tggtaaacag cattatgcag cagcctagaa atatactgat gtgtgtttgt
     3421 tcccgaccgg ctgttaactc atatacgaca gggtatgcac tgatgattat ggccggaata
     3481 aacaaccatg catttgtctg attctcaact tcggcagcag ctttgatatc tacaagtatt
     3541 ttttgtgtgg tacctacctt ccagttcttg ctctgcttta aagctttcag ctttcgatga
     3601 taccttgtat agggtacaaa aggtactata atcgcaaaaa taagccaata catcaaaatt
     3661 agcatcatga ttgaagagcg atcgacacaa aaaacagcaa agggtggaat tgctaatagc
     3721 aaaacaaact tacgcagcgc gtatttaaac tggttacaaa tatctattaa atctgaatca
     3781 tttaacgcca agcgcggtaa tgtaacacca agaataatat ttttccgtgg tttggcttca
     3841 tttattagta caaagtacat aatcggcagg atagggtaga agaggatcaa cataaaaact
     3901 ttaaaaaaac tcatcttgtc ccctccatct ctttactaat ccggttaaac aattcaagca
     3961 tttctgtttc gctgatgcct ttgacttttg cttcggatag aagcagcttt aactctttag
     4021 tcagtttagc cagtgtttct gctttgctgt catagggaag actgactttg gtaccacttc
     4081 gccggtcaat agagatgatc ccttcctgtt tcagcaactg ataagcttta ttgactgtca
     4141 ttgcattaat tcctgattca ttggccagag ctcgaattgt tggcagttta tcaccgtgat
     4201 ttagcgttcc ctcagctata gccagcaata tctggtttct gatttgtaga taaatgggcg
     4261 tctcactgga aaaatccaaa gctaaaatca tcgtcaacac tcccttgtat tatatattat
     4321 aatacatgta atgcatggta gacaagataa taatcagttt tttaacaggt actgaaaaac
     4381 aagtaatagt ttaaacaatt gcttgttagt aggaaaattg ccagttaggt gtgcaggatg
     4441 gtagtgtaat tatgccagta tataccagtt gccattaagg ttaaaatgtg atacactgaa
     4501 actcgtgccg caagacataa acattttact tgcgtacggg ttagccagga cttactttac
     4561 catgaaatgg atgttgacat gagagccagg gcatggtaag ataatattcc ggtcgtgaga
     4621 gcggcaagga tgtaaaacac agagtattca gaagcaagca gaacgcgaag caagacctgg
     4681 aggcaccgca ggcgtatacg atatacgttg aggatgccgc aaggttgaag cgacaaagag
     4741 atgcgcagct gatggacgat ctggagctct ttgaaaactg aacaggaata tgtaacgcca
     4801 tgcgaagtac acatcgatac attcgatata attggcagtt caaaagcaag tagtgcgagg
     4861 cgcaagaggt tccggcagcg gaggcgtatc gataatacgt tgatgatgac gggttctcgt
     4921 agcaacgacg caatacgaag cttttcaaca gtcaatacat tacgcttcat caaggcagtg
     4981 ctactagtgc tgaaccggat gagcaacttt ggtttcaaat aagagccaag aacaatctgt
     5041 aaacgggaag cttaatcgct tttcactata ttacggagag tttgatcctg gctcaggacg
     5101 aacgctggcg gcgtgcttaa cacatgcaag tc
//
LOCUS       DASRMS010000229         1563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_64133, whole genome shotgun sequence.
ACCESSION   DASRMS010000229 DASRMS010000000
VERSION     DASRMS010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1563
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64133"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="VFC74_10930"
     CDS             complement(<1..866)
                     /locus_tag="VFC74_10930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013362180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease"
                     /protein_id="HZK25877.1"
                     /translation="MTEYKAIAESNNFIVLDKYTKQLQVAESYQSEDELERELVQDLV
                     NQGYEYLPTLNNPQAMLANVREQLQVLNKVTFSEGEWQRFVETYLDKPSDSITDKTRK
                     IHDDYILDFVFDDGRIQNIYLVDKNNITRNKLQVIKQFEQTGSQANRYDVTILVNGLP
                     LVHIELKKRGVAIREAFNQVHRYSKESFNSDNSLYKYLQLFVISNGTDTRYFANTTQR
                     NKNSFDFSMNWAKADNSLIKDLKDFTATFFQKNTLLNVLLHYSVFDVSDTLLVMRPYQ
                     IAATERILWKIK"
     gene            complement(954..>1563)
                     /locus_tag="VFC74_10935"
     CDS             complement(954..>1563)
                     /locus_tag="VFC74_10935"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HZK25878.1"
                     /translation="YNYYRDQLLSFEEGEVEWKTLGDVAQLRRGRVMSKGYLVENAGK
                     YPVYSSQTANNGLIGRIKTYDFEGEFVNWTTDGANAGTVFYRTGKFSITNVSGIIKVS
                     NASQLNCKYLYYWLSIEAKKHVYSGMGNPKLMSNQVAKIPVPIPPLAEQNRIVAILDK
                     FDALTTSITEGLPREIELRQQQYEYYRDLLLSFPKPSDKQVA"
BASE COUNT          381 a          367 c          350 g          465 t
ORIGIN      
        1 ctttttatct tccataaaat acgctcggta gcggcaatct gatacgggcg cattaccagc
       61 agggtatcgc tcacatcaaa caccgagtaa tgcagcaaca cgttcagcaa ggtgtttttt
      121 tgaaagaaag tggcggtaaa atcttttaaa tctttaatca ggctgttgtc ggccttggcc
      181 cagttcatgc tgaagtcaaa actgttttta ttacgttggg tggtgttggc aaaataacgg
      241 gtgtcggtgc cgttagaaat gacaaacagc tgcaaatact tatacagcga gttgtctgag
      301 ttaaagctct ccttactgta gcgatgcacc tgattaaaag cctcgcgaat ggccacacca
      361 cgttttttca gctcaatatg caccagcggc aaaccattca ccagaatcgt cacatcgtag
      421 cgattagcct ggctgccggt ttgctcaaac tgcttaatca cctgcaactt gttgcgggta
      481 atgttgtttt tatcgaccag gtaaatattt tgaatgcggc catcgtcaaa cacaaaatcc
      541 agaatataat catcgtgaat tttacgggtt ttgtcggtaa tgctgtcgct gggtttgtct
      601 aagtaggttt caacaaaacg ctgccattca ccctctgaaa aggtcacctt gttcaatact
      661 tgcaactgtt cccgcacatt ggccagcatg gcttgtgggt tgttcaaagt aggcaggtat
      721 tcatagccct gattgaccaa atcctgaacc agctcgcgtt ccagctcatc ttcactttgg
      781 tagctctcgg ccacttgcag ttgcttggtg tatttatcca acacaataaa gttattggat
      841 tcggcgatgg ctttgtattc cgtcacggct gtttctcctc accgccggcc ttcgcacggc
      901 cggccagtgt tgttattttg atatttgacc atttcgagcg ggtaaaaggc atattaggcc
      961 acctgtttat cactcggctt agggaagctc aacagcaggt cgcggtagta ttcgtactgc
     1021 tgttggcgca gctcgatttc gcggggtaag ccttcggtga tggaggtggt gagggcatcg
     1081 aatttatcaa ggatagcgac aatgcggttt tgttcagcca atggtgggat ggggacgggt
     1141 atcttggcaa cctgattgct catgagtttg gggttgccca tacctgagta aacatgcttc
     1201 tttgcttcaa ttgaaagcca gtaatataga tatttgcagt tgagctgaga ggcattactg
     1261 acttttatta ttccgctcac attagtaatt gaaaacttgc ctgttctata aaaaacggta
     1321 cccgcattag cgccatctgt tgtccagtta acaaattctc cttcaaagtc gtatgttttg
     1381 attctcccaa ttagtccgtt attagcggtc tgggagctat atactggata cttcccagca
     1441 ttttccacca agtatccctt tgacataaca cgaccacgcc ttagctgcgc cacatccccc
     1501 aacgtcttcc actccacttc cccttcttca aaactcaaca actggtcgcg atagtagttg
     1561 tat
//
LOCUS       DASRMS010000230         1186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_669570, whole genome shotgun sequence.
ACCESSION   DASRMS010000230 DASRMS010000000
VERSION     DASRMS010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1186
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669570"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1186
                     /locus_tag="VFC74_10940"
     CDS             <1..>1186
                     /locus_tag="VFC74_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25879.1"
                     /translation="IKTNNMSTKSGEVISGQVDKLPLLKYFKHPVESVSELYRRYPMG
                     GEFGWFTFVYDLKTFAYWDIELKNWRVITGSGGLEQMPYIGTNKNWWLDDVDTGWKAV
                     FEFSDFTEENIDLFMRPALEAAETADTAADRADTSANAADAATASTNQAIENANIATG
                     KANTATTEANTARDNANTAAGNADEKASMASAAASNANAAAAAANTAQGNAIDAASGA
                     WIAVEKSELASSNANAAASTANTAASVANTATSEANTARDRANTSADNADEKAAIANT
                     AASRANDAANEIDETVIPNSQQATADAIGAKNDYYDNVKPDIEAQGLYAKEQGDYAKA
                     QGEAVEGRVSLINNNTLTIEEVTAMAIVAMQNRIVALEKILSEGILDKIKIMNLDIID
                     NFN"
BASE COUNT          435 a          224 c          298 g          229 t
ORIGIN      
        1 aataaaaaca aataatatga gcacaaaaag cggagaggta atatcgggac aggtagataa
       61 gctccccttg ttaaagtact tcaagcaccc tgtagaaagc gtatcagagt tatacaggag
      121 atatccaatg ggcggtgagt ttgggtggtt cacgtttgtg tatgatttaa aaacatttgc
      181 gtactgggat attgagctaa agaattggcg tgtaattact ggatcaggcg gactggaaca
      241 gatgccatac ataggcacga acaagaactg gtggctggat gacgttgata ccggatggaa
      301 ggctgtattt gaattttcgg acttcacgga agagaatata gatcttttta tgcgacctgc
      361 actagaggca gcagagactg cagatacagc agcagataga gcagacacaa gcgcaaacgc
      421 agcagatgca gcaacggcat caaccaacca ggctatagag aatgcaaaca tagcaacagg
      481 gaaagccaat acagcaacca cagaggcaaa tactgcacga gataatgcaa atacagccgc
      541 aggcaatgca gacgagaaag ccagtatggc tagtgcagca gccagtaatg caaatgcagc
      601 agcagcagca gcaaacactg cacaaggtaa tgcaatagat gctgctagtg gggcgtggat
      661 tgcagtagaa aaatccgagc ttgcatcaag taatgcaaat gcagccgcaa gtactgctaa
      721 cactgcagca tcagtcgcaa acacagccac ctcagaagca aacactgcca gagacagagc
      781 aaacacaagt gcggacaatg ctgatgaaaa agcagcgata gctaatactg cagcaagcag
      841 ggcaaacgat gcagctaatg agatagacga aacagttatc ccaaactcac aacaagccac
      901 agcagatgca ataggggcaa agaatgacta ttacgacaat gtaaagcctg atattgaggc
      961 acaggggctt tatgcaaaag agcaaggtga ttatgcaaag gcacagggtg aggcggttga
     1021 agggagagtt agcttgataa ataataacac cttaacaata gaagaggtaa cagcgatggc
     1081 aattgtagca atgcaaaata ggatagttgc acttgaaaag attttatcgg aaggtattct
     1141 tgacaagata aaaataatga accttgatat aattgacaat tttaat
//
LOCUS       DASRMS010000231        16165 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_936407, whole genome shotgun sequence.
ACCESSION   DASRMS010000231 DASRMS010000000
VERSION     DASRMS010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 16165)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16165)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16165
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_936407"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFC74_10945"
     CDS             complement(<1..579)
                     /locus_tag="VFC74_10945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HZK25880.1"
                     /translation="MAKQKFERNKTHVNIGTIGHVDHGKTTLTAAITSCLSTAGFAQK
                     QAFDQIDKAPEERERGITISTSHVEYETEKRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLARQVGVPYIVVFMNKADQVDDDELLELVEMEVRDLLTE
                     YEFPGDDTPVVIGSALKALEHGCGSRDCPDCKV"
     gene            complement(612..2687)
                     /gene="fusA"
                     /locus_tag="VFC74_10950"
     CDS             complement(612..2687)
                     /gene="fusA"
                     /locus_tag="VFC74_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020491310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HZK25881.1"
                     /translation="MTRTISLGRTRNIGIMAHIDAGKTTTTERILFYTGRVHKLGETH
                     DGAATMDWMVQEQERGITITSAATTAFWHDHVINIIDTPGHVDFTVEVERSLRILDGA
                     VGVFCAKGGVEPQSETVWRQADKYHVPRIAYVNKMDITGANFFNVLESMKQRLNANVV
                     PIQLPIGVEDQFIGIVDLIRMKSIIYADDLGTHSDETEIPTEMLELAQEYREKMLEAA
                     AEFDEDLMMSYLEGEEISEEQIRAALRKGTCAVQLVPVLCGSSYKNKGVQPMLDAVIH
                     YLPSPLDVPPVQGMNLETEEEVVRVASDEAPFAALAFKIMADPYVGKLTFARVYSGTL
                     ASGSYVYNSSKGKRERVGRILRMHANHREEIKEIRSGDIVAIVGLKNTTTGDTLCNEQ
                     APVILENITFPEPVIDVAIEPKTKADQEKMSLSLVKLSEEDPTFRARTDEETGQTIIS
                     GMGELHLEIIIDRLLREFKVGANVGKPQVAYKETIRSHARVEGKFVRQSGGRGQYGHC
                     WLELEPLESGQGYVFENKIVGGVIPREYVPAIDAGCKEAMNTGVLAGYPVIDVKVAVV
                     DGSYHDVDSSEMAFKIAGSMAFRAGVLKAKPALLEPMMGVEITVPEEYMGDVMGDVNS
                     RRGRIEGMDPRIGVQVIRAKVPLSEMFGYATDLRSRTQGRGTYSMEFSHYEEVPKSLA
                     AEIIEKQKG"
     gene            complement(2759..3229)
                     /gene="rpsG"
                     /locus_tag="VFC74_10955"
     CDS             complement(2759..3229)
                     /gene="rpsG"
                     /locus_tag="VFC74_10955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HZK25882.1"
                     /translation="MSRKGPASKRDVLPDPLYNSKLITKFINSLMLDGKKGVAQAIFY
                     DAMDTIREKTGSDPLEVFETAVKNVSPVLEVKARRVGGANYQVPVEVSAHRRSILALR
                     WLTRYSRERGERTMSERLAAELLDAVNSTGGAFKKKEDTHRMAEANKAFAHYRW"
     gene            complement(3262..3636)
                     /gene="rpsL"
                     /locus_tag="VFC74_10960"
     CDS             complement(3262..3636)
                     /gene="rpsL"
                     /locus_tag="VFC74_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HZK25883.1"
                     /translation="MPTLNQLVRKGREQVVKKSTAPALEKSPQKRGVCTRVSTTTPKK
                     PNSALRKIARVRLTNGIEVSTYIPGIGHNLQEHSVVLVRGGRVKDLPGVRYHLVRGAL
                     DAAGVENRQQSRSKYGAKRKKK"
     gene            complement(3710..3964)
                     /locus_tag="VFC74_10965"
     CDS             complement(3710..3964)
                     /locus_tag="VFC74_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HZK25884.1"
                     /translation="MDLEVLKQAQQTVTGTKQTIKAVNSGQAENVYLARDADERVVQP
                     IITACSANNVPVSYVESMAELGKACQIKVKAAAAAILRTD"
     gene            complement(4084..7731)
                     /gene="rpoC"
                     /locus_tag="VFC74_10970"
     CDS             complement(4084..7731)
                     /gene="rpoC"
                     /locus_tag="VFC74_10970"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015358019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HZK25885.1"
                     /translation="MLDVNNFDALKIGLASPEQIRNWSRGEVKKPETINYRTLKPERE
                     GLFCEKIFGPQKDWECHCGKYKRVRYKGIVCDRCGVEVTRAKVRRERMGHIELAAPVS
                     HIWYFKGIPSRMGLLMDMSPRALEKILYFAAYVVIDPGDTGLVKKQLLTEMEYREHRE
                     KYDHLFRVGRGFKADMGAEAVKKLLQEIDLEEMVKELRAELRSATGQKRIRATRRLEV
                     AEAFRQSGNNPAWMILEAIPVIPPDLRPMVQLDGGRFATSDLNDLYRRVINRNNRLKR
                     LLDLGAPDIIVRNEKRMLQEAVDALVDNGRRGRPVTGPGNRPLKSLSDMLKGKQGRFR
                     QNLLGKRVDYSGRSVIVVGPELKLYQCGLPKEMALELFKPFVMKRLVNEGFAHNIKSA
                     KRMVEKVRPEVWDVLEVVITDHPVMLNRAPTLHRLGIQAFEPVLVEGRAIKIHPLVCT
                     AYNADFDGDQMAVHVPLSAEAQAEARLLMLSAQNILNPKDGRPVVTPTQDMVLGSYYL
                     TLEKEGARGEGKTFSSVEETLLAYQTGVIELQARIAVDITDFSKVFTGREELRRLPGP
                     KLFITTVGKLIFNDIFPVDFPYLNNPDFSRPVDEDDFILQKGQNIPDLIKQHSLSDAV
                     KKSFLGTIVAMCYRKYGNARTAEILDDIKQLGFTYATRAGITVGVVDVTIPEEKQEIL
                     AAAEKSVEKIEMQFRRGLITNDERYDRVIEIWTKAKDDVTKALMSSLDPLNPIYMMAN
                     SGARGNISQITQLAGMRGLMADPTGRIIDLPIKANFREGLTVLEYFISTHGARKGLAD
                     TALKTADSGYLTRRLVDVAQDVIVREDDCGTNQGVIVSEIHEGSEVIEDLHDRINGRF
                     ALADILDAETGELLVAKDEFINEDLADTIIAKGITKVQIRSVLTCHTRHGVCKKCYGR
                     NLATGRIVDVGEAVGIIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVEELFEAR
                     KPKGLAIIAEEDGVAQIVEARQRREVQVTPDDGETKSYPIPYGSRVKVKDGDQVEAGQ
                     ELTEGSVNPHDLLRVKGPRGVQIYLLHEVQRVYRLQGVDINDKHIEVMVRQMLKKVKI
                     EEAGDTELLPGGLIDSFIFEDLNEEILAQGGEPAVARPLLLGITKASLATDSFLSAAS
                     FQETTRVLTEASIKGKSDPLLGLKENVIIGKLVPAGTGMNRYRNIRILPLVELPEQAE
                     AAKNSELTREE"
     gene            complement(7776..11549)
                     /gene="rpoB"
                     /locus_tag="VFC74_10975"
     CDS             complement(7776..11549)
                     /gene="rpoB"
                     /locus_tag="VFC74_10975"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HZK25886.1"
                     /translation="MFKVIDAGTRERRTYARIDEVLDLPNLIEIQQTSYKWFLEEGLQ
                     EMFRDISPIQDFTGNLVLEFIGYFLGEPKYSVDECKERDATYSVPLRMRVRLINKETG
                     EVKEQEVFMGDFPLMTENGTFIINGAERVIVSQLVRSPGVYFKSQIDFSGKVLHSATI
                     IPNRGAWLEYETDANDVIYVRIDRTRKVPVTVLLRAIGYGTNNDILRLYDHDTRILAT
                     LEKDHTDSFESGILEIYKRLRPGEPLNVDNARSLFESLFFEPKRYDFASVGRYKINKK
                     FALAPRIKQQKLLENVVDAEDNILARAGEIITTEMAESFEGKGIVRVRIEHNEREVTV
                     TNNGQIPDTTKHLTREDIVATIGYMLNLADGIGDVDDIDHLGNRRLRSVGELLQNQFR
                     IGLSRMERVVRERMTIQDVEAITPQALINIRPVIASIKEFFGSSQLSQFMDQTNPLAE
                     LTHKRRLSALGPGGLSRERAGFEVRDVHHSHYGRMCPIETPEGPNIGLIGSLSTFARI
                     NEYGFIETPYRKVDKEKGRVTEKIVYLTADDEDHYTVAQANAELDDEGYFLSQRVTCR
                     HRHETITRPPSAIDFMDVSPKQLVSVATALIPFLENDDANRALMGANMQRQAVPLLKA
                     DAPLVGTGLEYKTARDSGVVILADKNGEVVYVTATMIVIRRSEDPDAPNRLINGRTGE
                     IFPGEPRLNYQRGCDIYTLQKFKRSNQGTCMNQRPIIHKGQQVAAGDVIADGPSTDQG
                     ELALGRNVLVAFMPWEGYNYEDAILLSEKAVKEDVFTSIHIEEYESEARDTKLGPEEI
                     TRDIPNVGEDALKDLDERGIIRVGAEVRAGDILVGKVTPKGETELTAEERLLRAIFGE
                     KAREVRDTSLRVPHGEAGMVVDVKVFSREQGDELPPGVNQLVRAYVAQKRKISEGDKM
                     AGRHGNKGVVARILPEEDMPFLPDGRPVEIVLNPLGVPSRMNIGQILETHLGWAAKVL
                     GIHIATPVFDGAREEDVFTAFTEAGLPETGKSVLYDGRTGDPFDNQVTVGYIYMLKLA
                     HLVDDKIHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEILTVK
                     SDDVVGRVKTYEAIVKGENIPEPGVPESFKVLVKELQSLALDVKIIDEETGEMEIRES
                     DEDGEVQDLGVNIEGTEDENAPNTKLVLEEDDEDDLDSEDLKDAADDELSDNELKIFP
                     LTAEDEDSEEDDELVLDDELTDLDLEDELDEDEDDLDADLDDLADDDDGDDDE"
     gene            complement(11848..12222)
                     /gene="rplL"
                     /locus_tag="VFC74_10980"
     CDS             complement(11848..12222)
                     /gene="rplL"
                     /locus_tag="VFC74_10980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018214412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HZK25887.1"
                     /translation="MSKVVEVLDIVKEMTVLELSELVKAFEEEFGVSAAAPVAMAAMP
                     VAGDAPAVEEQTEFDVILLAAGEKKVATIKVVREVTGLGLKEAKEVVDSAPKAVKEKI
                     SKEDAEALKAKLEEAGASVELK"
     gene            complement(12275..12805)
                     /gene="rplJ"
                     /locus_tag="VFC74_10985"
     CDS             complement(12275..12805)
                     /gene="rplJ"
                     /locus_tag="VFC74_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HZK25888.1"
                     /translation="MSISKETKRQVVAEVKEDLSNAKTAIITDYRGLNVQQITTLRRA
                     LRAENVKYTVAKNTLVRIAAHELGYEGLDLYLEGPTAIAFGFEDPVAPAKVLSKYAKE
                     FDKLELKGGLLEGGLIDASRIKALADLPAREVLLAHVAGAFASPMTSFAGAAQALLRK
                     FVGTLDAVREKKAAEA"
     misc_feature    complement(12882..13029)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(13083..13793)
                     /gene="rplA"
                     /locus_tag="VFC74_10990"
     CDS             complement(13083..13793)
                     /gene="rplA"
                     /locus_tag="VFC74_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011928387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HZK25889.1"
                     /translation="MAKHGKKYLDARKLIDREQPYEPEEALALAQKTATTKFDGTVEL
                     AVRLGVNPKHADQQVRGAVVLPAGIGKAVTVAVFAKGEKAKEAEMAGADFVGAEDLVA
                     KIQEGWFDFDVAVATPDMMGTVGKLGRILGPRGLMPNPKTGTVTMDVARAINEIKAGK
                     VEYRVDKNSIIHVPIGKVSFAVEKLLENFYTIIDTLQKVKPAAAKGQYLRSITVSTTM
                     GPGVPISTQKAAQLGKVK"
     gene            complement(13853..14278)
                     /gene="rplK"
                     /locus_tag="VFC74_10995"
     CDS             complement(13853..14278)
                     /gene="rplK"
                     /locus_tag="VFC74_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013381851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HZK25890.1"
                     /translation="MAKKVKGLVKLQIPAGKATPAPPVGPALGQHGVNIMGFCKEFNE
                     RTAQQAGLVIPVVITIYEDRSFSFITKTPPAAVLLKKAAGLDTASGEPNKVKVATLSR
                     DKVRKIAELKMEDLNANDVEAAMRMVEGTARSMGITIQK"
     gene            complement(14301..14825)
                     /gene="nusG"
                     /locus_tag="VFC74_11000"
     CDS             complement(14301..14825)
                     /gene="nusG"
                     /locus_tag="VFC74_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013119195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HZK25891.1"
                     /translation="MSKNWYVVHTYAGYENKVKTNLEKRVESMEMQDKIFRVLVPMEK
                     EFEIKNGKRKSTLKKVFPGYVLVEMTITDDSWYVVRNTPGVTGFVGPGSKPIPLSESE
                     IAQILKQMGMDEPKPKIDFVTGESVRVVEGPFANFIGKVEEVLPDRRKIKVLVSMFGR
                     ETPVELEYFQVEKL"
     gene            complement(14854..15048)
                     /gene="secE"
                     /locus_tag="VFC74_11005"
     CDS             complement(14854..15048)
                     /gene="secE"
                     /locus_tag="VFC74_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010244917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HZK25892.1"
                     /translation="MAANVKGLTKYFKEVRQELKKVHWPNRRELTVYTSMVLTTVFFI
                     GIFFWLLDAGFTELLKLILQ"
     gene            complement(15128..15277)
                     /gene="rpmG"
                     /locus_tag="VFC74_11010"
     CDS             complement(15128..15277)
                     /gene="rpmG"
                     /locus_tag="VFC74_11010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HZK25893.1"
                     /translation="MRVKITMACGECKRRNYITNKNKRTTPDRIELNKYCPFCKRHTA
                     HKETK"
     gene            complement(15571..>16165)
                     /locus_tag="VFC74_11015"
     CDS             complement(15571..>16165)
                     /locus_tag="VFC74_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015755958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HZK25894.1"
                     /translation="YIPDPERDVDKPFLMPVEDVFTITGRGTVATGRVDRGVVKVQDE
                     VEIVGLSEKPRKTVVTGVEMFRKIMDQAVAGDNIGCLLRGIDRDSIERGQVLAKPASI
                     KPHTNFMAEVYVLKKEEGGRHTPFFQGYRPQFYLRTTDVTGVITLPEGVEMVMPGDNI
                     QMKIELITSIALEEGLRFAIREGGRTVGAGVVTSIIA"
BASE COUNT         4192 a         4100 c         3303 g         4570 t
ORIGIN      
        1 tactttgcaa tcggggcagt cacggctgcc acagccatgc tccagagcct tgagcgcaga
       61 accaatcacc acaggagtat cgtcaccagg gaattcatat tccgttaaga ggtcacgtac
      121 ttccatttcc acaagctcta acagctcgtc atcgtctact tggtcagctt tgttcatgaa
      181 aacaacaatg taaggtacgc caacctgacg ggccagcagg atgtgctcgc gtgtttgcgg
      241 catcgggcca tcagctgcgg aaacaactag gatcgcgccg tccatttgtg ccgcaccagt
      301 aatcatgttt ttaacatagt cggcgtggcc ggggcagtca acgtgcgcat agtgacgctt
      361 ttctgtttca tattccacgt gagaggtgga aatggtgata ccacgttctc tctcttcagg
      421 tgctttatcg atttgatcga aagcttgctt ttgggcaaag cctgcagtag agaggcagga
      481 tgtgatagct gcagtcagcg tggttttacc atggtcaacg tgaccgattg taccaatatt
      541 aacgtgggtt ttgtttcttt caaacttctg ttttgccatt gtaaggtgaa cctcctttaa
      601 ttcaacaaag attatccttt ttgtttttct attatttcag cagccaggct ctttggcact
      661 tcctcataat gtgagaactc catagagtag gtaccgcgtc cttgggtgcg agaacgtaga
      721 tctgtggcat agccgaacat ttccgacagc gggactttag cacgaatcac ctgtacaccg
      781 atacgtggat ccatgccctc gatgcgtccg cgacggctgt ttacatcacc cataacatcg
      841 cccatatact cctcaggcac tgttatttca acacccatca tcggttcgag aagtgcgggt
      901 ttggctttaa gtacaccagc tctaaaagcc atagaaccag caattttaaa ggccatttca
      961 gaagagtcaa catcatggta tgagccgtca acgacggcaa ctttaacgtc aatcaccggg
     1021 taaccagcca gaacacctgt attcatagct tctttacagc cagcatcgat ggcggggaca
     1081 tattctctgg gaataacacc gcctacgatt ttgttttcaa agacgtaacc ttgacccgac
     1141 tccaaaggct ctagctctaa ccaacagtga ccgtactgac cacggccgcc ggattgacgt
     1201 acgaattttc cttccacccg agcgtgacta cgaatggttt ctttgtacgc tacctggggc
     1261 ttaccaacat tagcaccaac tttaaattcg cgcaagagac gatcaataat aatttccaag
     1321 tgcagctcgc ccatccccga gataatggtc tgcccagttt cttcatcggt tctggcccta
     1381 aaggtggggt cttcttccga cagtttaact aaggagagag acattttctc ttgatctgct
     1441 ttggtcttgg gttcaatcgc cacatctatt accggttctg ggaaagtaat gttttccaga
     1501 ataacaggag cctgctcatt acaaagggta tcgcccgttg tggtattttt tagacccaca
     1561 attgccacaa tgtcaccaga tctaatctct ttaatttcct cacggtggtt agcatgcatt
     1621 cgtaggatac gtccaacccg ctctcgttta cctttggatg agttgtaaac gtaggaaccg
     1681 ctggcaagag taccggagta aacacgtgca aaggtgagct taccaacata gggatcggcc
     1741 ataattttaa aagctagggc tgcaaaaggc gcttcgtcgc tggcgacacg tacaacttct
     1801 tcttccgtct ccaagttcat accttgtact ggagggacat ccaacggtga aggcaagtaa
     1861 tgaataactg catccaacat tggttgcaca cctttatttt tatacgagga tccgcagagg
     1921 acaggaacca gctgcaccgc acaggtacct ttacgcaagg ccgcacggat ttgttcttca
     1981 ctgatctcct cgccttctaa atagctcatc atcaggtcct cgtcaaattc agcagcagct
     2041 tccagcattt tttcccggta ttcctgggcc agttcgagca tctcagtggg aatttcagtc
     2101 tcatcgctat gagtccccag gtcgtcagca tagataatgg atttcatacg aatgagatcc
     2161 acaataccaa taaattgatc ctccacaccg atcgggagct ggatggggac cacatttgca
     2221 ttcagccgct gtttcatcga ctctagtaca ttaaaaaagt tcgcacctgt aatgtccatt
     2281 ttattcacat atgcaatgcg cggcacatgg tacttatccg cttggcgcca gaccgtttcg
     2341 gactggggct caacaccacc tttggcacaa aaaacaccta ctgcgccgtc gagaatacgc
     2401 aggctgcgtt ctacttctac tgtaaaatca acgtgcccgg gtgtatcaat aatattaatg
     2461 acatgatcat gccaaaaggc agtggttgcg gcagaggtaa tggtaatacc acgctcctgc
     2521 tcctgcacca tccaatccat tgttgctgcc ccatcatgcg tttcacccag tttgtggaca
     2581 cgccctgtat aaaacaggat tcgttctgtt gttgtcgttt tgccagcatc aatatgtgcc
     2641 ataattccta tgtttcttgt cctgcccagg gaaattgttc ttgtcatttg gtcccctcct
     2701 ttcttagctt gatgttattt ttttgtaaaa cagtcaagac atttagtaca ccatcaacct
     2761 accagcgata atgagcgaaa gctttgttag cttcggccat gcggtgggta tcttctttct
     2821 ttttaaaagc tccacccgta ctatttacgg catcgagcag ttcggctgct agacgttctg
     2881 acatggttct ttcaccacgc tcccgggagt atcttgttaa ccaacgtaag gctaaaatac
     2941 tacgacggtg agccgaaact tctacgggca cttggtagtt agcaccacca acacgacgcg
     3001 cttttacttc tagcactgga gaaacgtttt taacggcagt ttcaaaaact tctagcggat
     3061 cgctgcctgt tttttcacgg atagtgtcca tcgcatcata aaaaatcgct tgtgctacac
     3121 cctttttacc atcaagcatt aacgaattaa taaatttagt gattaattta gaattataga
     3181 ggggatcagg caaaacatcc cttttagatg ccggtccttt acgagacata ccctaacctc
     3241 ctttcacagg ggttttttta tttatttctt tttacgtttt gcaccgtatt tagaacggga
     3301 ttgttgacgg ttttccacac cagcggcatc taaagcgcca cgaactaggt gataacgcac
     3361 accaggcaga tccttcacac gaccgccgcg cacaagcact actgagtgtt cctgtaagtt
     3421 atgaccaata ccgggaatat atgttgagac ttcaatcccg tttgttaaac gtacacgagc
     3481 aattttacgc agcgctgagt taggcttttt gggcgtggtt gtggagacac gagtacaaac
     3541 accgcgcttt tgtggagact tttccagagc aggcgcagtt gattttttca caacttgctc
     3601 tcttccctta cgtaccaatt gattcagagt cggcattcca ctccacctcc ttccttaaag
     3661 ctacaagtgt aaaccccatc cactccacag gggagtggat gggaaataat taatccgtgc
     3721 gaagaatcgc agcagctgct gccttcactt taatttgaca ggcttttcct agctctgcca
     3781 ttgattcaac atagctaaca ggcacattgt tagcactgca ggcggttata attggctgaa
     3841 ccacacgttc atcggcgtct cgagcaagat agacgttttc tgcctgaccg ctattaaccg
     3901 ctttaattgt ttgttttgta cctgttactg tctgttgggc ttgttttaaa acctcgaggt
     3961 ccatcaggca gcctccattg tttcctatta aaaacacact ttgatatttt accatcatca
     4021 agttcccttg tcaatcattt agtcttgttc tcttagcatt aatatctatc ttttacagag
     4081 cctttattct tcccttgtca attcgctgtt ttttgcagct tcagcttgtt cgggcagctc
     4141 gactaagggc aggatacgaa tgttgcggta acggttcatc ccggtacctg ctggtactaa
     4201 cttaccgata atcacatttt ccttcagccc gagcagcgga tcacttttac ccttgatcga
     4261 tgcttctgta agcacacgcg ttgtctcctg gaacgaagcc gccgataaga aggagtcagt
     4321 agccagtgag gctttggtga ttcctagtaa aagcggacgg gcgacggcgg gttctccgcc
     4381 ttgcgccagg atctcttcat tcagatcttc gaagataaag gaatcaatca aaccgcccgg
     4441 cagcagctcg gtatcgccag cctcttctat tttaactttc ttcagcatct ggcgcaccat
     4501 aacttcgata tgcttatcgt tgatatctac gccctgcaaa cggtaaactc gctgtacttc
     4561 atgcaaaaga taaatctgta cgccacgggg acctttcacg cgcagaaggt cgtgaggatt
     4621 aacagaaccc tcagtaagct cctgacctgc ctctacctgg tcaccgtctt ttactttcac
     4681 gcgcgaaccg taaggaatgg ggtatgactt tgtttcaccg tcatccggtg tcacctgtac
     4741 ttcacggcgt tggcgggctt ccactatctg cgctacgcca tcctcttcgg caatgatcgc
     4801 cagacccttt ggtttacgtg cttcaaataa ttcttctacc ctaggcaaac cttgcgtaat
     4861 atcatcacca gctacaccgc cggtatggaa agtacgcatt gttaattgag tgcccggttc
     4921 gccaattgat tgggcagcaa taatgccaac tgcttcacca acgtccacaa tacgtcctgt
     4981 ggccaagttg cgaccataac attttttaca gacaccgtgt cgggtgtgac aagttaaaac
     5041 cgaacgaatt tgtactttag taatgccttt ggcgataatc gtatcagcga gatcttcatt
     5101 aataaattca tctttggcga ctagcagttc acccgtttct gcatctaaaa tgtcggccaa
     5161 ggcaaagcgg ccattaatgc ggtcatgaag atcttcaatc acttcactac cctcatggat
     5221 ttcagatacg atcacacctt gattggtgcc acagtcgtct tctcgtacaa ttacatcctg
     5281 cgctacatcc accagacgtc tcgtgagata gcccgagtct gctgtcttta aagcggtatc
     5341 tgccaaacct ttacgggcac cgtgggtgga aatgaagtat tccaacactg ttaatccttc
     5401 acggaagttg gccttaatcg gcaagtctat aatgcggcca gttgggtcag ccatcaaacc
     5461 acgcatcccc gccaattggg tgatttgcga aatgttaccc cgggcaccgg agttagccat
     5521 catataaatc gggttaagcg gatctagtga gctcattaag gctttagtta catcatcttt
     5581 agcctttgtc cagatctcaa tgacacggtc gtagcgttca tcattggtaa ttaaaccacg
     5641 gcggaattgc atttctattt tttctactga cttttcagca gcagcgagaa tttcttgctt
     5701 ttcctccgga atagttacat caacaacacc cacggtaatc ccggcccgtg tggcataggt
     5761 aaagcccagc tgcttaatat cgtctaaaat ctctgcagtg cgggcgttac catatttgcg
     5821 ataacacatg gccacgatcg tacccaagaa agacttttta accgcatcac taagggaatg
     5881 ctgcttaatc aggtcgggga tgttttgtcc tttttgcagg ataaaatcat cttcgtccac
     5941 tggtctgctg aagtcagggt tattaaggta agggaaatcc actggaaaga tatcgttaaa
     6001 gattagtttg cctaccgtgg tgatgaaaag tttaggtcca ggcagacggc gcaattcttc
     6061 acgtccggta aataccttgg aaaaatcggt gatatcaaca gcaatacgtg cctgtagttc
     6121 gattactccc gtttgatatg ccaaaagcgt ctcttcgacg gaagaaaaag ttttgccttc
     6181 gcctctagcc ccttcttttt ctagcgtcaa gtaatagctg cccaaaacca tatcttgcgt
     6241 gggtgtcact acaggacgac catctttggg gtttaagatg ttttgtgctg aaagcatcag
     6301 gaggcgtgct tctgcttgtg cttctgccga taacggtacg tgtaccgcca tttggtcacc
     6361 atcaaagtca gcattatacg ctgtacagac tagcgggtgt atcttgatgg cccggccttc
     6421 aaccagaacc ggttcaaaag cttgaatacc taaacggtgc aaggttggtg ctcggttaag
     6481 catcacagga tgatctgtaa ttacgacttc cagcacatcc cagacttccg gccgcacttt
     6541 ctctaccatg cgtttagcac ttttgatatt gtgggcaaaa ccttcattga ccaagcgctt
     6601 catgacaaaa ggcttaaaaa gttccagggc catttctttg ggcagaccgc attgatataa
     6661 cttcaattct ggtccaacta caatcacgga acgaccggag tagtccacgc gcttacctaa
     6721 caagttctgc cggaagcgcc cttgtttccc tttgagcatg tcagagagag atttcagcgg
     6781 gcggttgcct ggtcccgtaa caggccggcc acggcggcca ttatcgacta aggcatcaac
     6841 tgcttcttgc agcatgcgtt tttcgttacg cacaataata tcaggggcac ccagatctag
     6901 taaacgcttt aagcggttat ttctgttaat gactctgcgg taaaggtcat tcaaatcaga
     6961 ggtggcaaaa cgtcctccat ccaactgcac catgggacgt aagtctgggg gaatgactgg
     7021 aattgcctct agaatcatcc aggcaggatt gttgccagat tgccggaaag cctctgctac
     7081 ttctaagcgt cgcgtagcac gaattctttt ttgtcctgtg gcagaacgta attcggcgcg
     7141 caattctttg accatctctt caaggtcgat ctcctgcagc agctttttta cagcctctgc
     7201 acccatatca gctttaaaac cacgacctac tctaaacagg tgatcatact tttcccgatg
     7261 ctctcgatat tccatttccg taagcagctg ttttttgact aaaccggtat caccgggatc
     7321 gatgacgacg taggcggcaa agtaaaggat tttttccaag gcacgcggcg acatatccat
     7381 caacagtccc atccggctgg gaattccttt aaagtaccag atatgggaga ctggagcagc
     7441 taattcgata tgccccatgc gttctcttct tactttagcg cgtgtaactt ctacaccgca
     7501 gcgatcacaa acgatacctt tataacggac gcgcttgtat ttaccacaat gacattccca
     7561 atccttctgc ggcccgaaaa tcttctcgca gaataagcct tcccgttctg gctttaaggt
     7621 tcggtagtta atcgtctcag gctttttcac ttctcctcgc gaccagttgc gaatctgctc
     7681 gggtgaggct agaccgattt tcaaagcatc aaaattgtta acatctagca agggcatttc
     7741 ctccctttct cttgatccac agataaccca cgctgttatt catcatcgtc accatcgtca
     7801 tcatcagcca agtcatctaa atcagcatcc aaatcatctt catcttcatc aagttcatct
     7861 tccaaatcaa ggtcggtaag ctcatcatcc aaaactagtt cgtcatcttc ttcgctatct
     7921 tcatcttcgg cagttaaagg aaatattttt agttcattat ctgataattc atcgtctgca
     7981 gcgtctttta aatcttcact atcaagatcg tcttcgtcat cttcttctaa gaccaattta
     8041 gtatttggcg cattctcatc ttcggtgcct tcaatattaa cacctaaatc ctgcacttca
     8101 ccatcttcat cgctttcgcg aatttccatt tcgccagtct cttcatcaat gatttttacg
     8161 tctaaggcta gactttgcag ttctttcacc aaaaccttaa aggattccgg cacgccaggt
     8221 tcaggaatat tttccccttt gacaatagct tcatatgttt taacccgacc aaccacgtcg
     8281 tcagacttaa ccgtcagaat ttcctgcagg gtataagctg caccgtaggc ctcaagggcc
     8341 catacttcca tttcaccaaa gcgttggccg ccaaactggg ctttacctcc cagcggctgt
     8401 tgtgtgacta aagagtaggg tcccgtggaa cgagcatgga ttttatcatc aactaaatgt
     8461 gccagtttaa gcatatagat atagccgaca gttacttgat tgtcaaaggg atcaccagtt
     8521 cgtccatcat agaggactga tttgccagtt tcaggcagac ctgcttcggt aaacgctgtg
     8581 aagacgtctt cttcccgggc gccatcgaaa acaggtgtgg caatatgaat accaagtact
     8641 tttgcggccc agcccaagtg tgtctctaat atctgaccaa tattcatccg agagggtaca
     8701 ccaagcggat taagtacaat ctccactggt cggccatcag gcaagaaagg catatcttct
     8761 tcaggtaaaa tacgggcaac tacacctttg ttaccatggc gacccgccat tttgtcccct
     8821 tcagaaattt tgcgtttttg tgctacgtat gcacggacca gttgattgac accaggtggc
     8881 aattcatcgc cttgctcacg cgaaaagact ttaacgtcaa cgaccatccc cgcttcacca
     8941 tgggggacac gtaaggaagt atcacgtact tcacgtgctt tttcgccaaa gatagcgcgc
     9001 agcaaccgtt cctctgctgt taattctgtt tcacccttag gtgttacttt acctacgagg
     9061 atatcgccag cgcgtacttc ggcaccgacg cgaataatac cacgttcatc tagatctttt
     9121 aaagcatcct cacctacatt gggaatatca cgagttattt cttccggtcc aagtttggta
     9181 tcacgtgcct ctgattcata ctcttcaata tggatagagg taaagacatc ttcttttacc
     9241 gccttctcac tcaggagaat agcatcttcg tagttatagc cttcccaagg cataaatgct
     9301 actagcacat ttcgccctaa ggccagttca ccctgatcag tagacgggcc atcagcaatc
     9361 acatcacccg cggcaacctg ttgccctttg tggataatgg ggcgctggtt catacatgtt
     9421 ccttgattgg aacgcttaaa tttttgtagg gtatagatgt cacaaccacg ttgatagttg
     9481 aggcgtggct ctccggggaa gatttcaccg gtacgcccat tgatcaggcg gttgggggca
     9541 tcaggatctt cgctgcggcg aataactatc atggtggcag tgacatagac aacttcacca
     9601 tttttatcag ccaagattac aaccccggaa tcacgggctg ttttgtactc caagcctgtc
     9661 ccgacgagcg gggcatccgc cttcagcagc ggtactgcct gacgctgcat gtttgcaccc
     9721 atcagagcac ggttagcatc gtcattttct aaaaaaggaa ttagggccgt agccaccgaa
     9781 actaattgtt taggcgagac atccataaaa tcaatggcag agggcggccg tgtaatcgtc
     9841 tcatgccgat gtcggcaggt gacgcgctga cttaagaaat atccttcatc atcaagttca
     9901 gcattagcct gagccactgt ataatgatct tcatcatcgg ccgtcagata aacaatcttt
     9961 tctgtcacac gccccttttc tttatcaact ttgcggtaag gcgtttcgat aaagccatat
    10021 tcattaatac gggcgaaagt tgataaagaa ccaatcaggc caatgttggg accttcaggt
    10081 gtctcaatgg gacacatccg accatagtga gagtggtgca cgtcacgcac ttcaaatcca
    10141 gcacgctctc gactcaagcc gcccggaccc agtgcagaaa gacgacgttt atgtgtcagt
    10201 tcggccaaag gattggtttg atccataaat tgtgataact gactcgagcc aaaaaactct
    10261 ttgatggaag ctattacagg acggatgtta atcaaggcct gcggcgtaat agcctccaca
    10321 tcctgaattg tcatacgttc acgtaccacg cgttccattc tcgataaccc aatgcggaat
    10381 tgattctgca gtaattcgcc gacactgcgc agtcgacgat tacccaaatg atcaatatcg
    10441 tccacatcgc caatcccatc cgctaaatta agcatatagc cgatggttgc cacgatatct
    10501 tcccgagtta aatgtttagt cgtatcggga atctgtccgt tattagtcac tgtgacttcc
    10561 cgttcgttat gctcgatacg cacacggaca atacctttgc cctcaaacga ctccgccatt
    10621 tccgtagtga tgatctcacc ggcgcgagcg agaatattat cttccgcatc aactacattt
    10681 tctaataatt tttgctgctt aatacgcggc gctaaagcaa attttttgtt aattttataa
    10741 cgtccgacac tggcaaaatc ataccgttta ggttcgaaaa atagtgactc gaaaagtgaa
    10801 cgggcattat ctacgttgag aggctcacct ggacgcaagc gcttatagat ctctaaaatc
    10861 cctgattcaa aagaatcggt atgatctttt tctagtgttg ctaaaatgcg tgtatcatga
    10921 tcataaagtc gtaaaatatc attattggtg ccatagccaa tagcacgcaa aagcaccgta
    10981 accggcactt tcctcgtacg atcaatccgt acataaataa catcgttagc atcagtttca
    11041 tattccaacc aggcaccacg gttaggaata atggttgcgg aatgcagcac cttaccactg
    11101 aagtcaattt ggcttttaaa atagaccccg ggcgagcgaa ctaactgact aacaattacc
    11161 cgctcagcac cattaataat aaaggtaccg ttttccgtca tcagtggaaa atcacccata
    11221 aatacttctt gttctttcac ttcgccagtt tctttgttga tcaggcgaac ccgcatgcgt
    11281 aacggcaccg agtaggtagc gtcgcgctct ttgcattcat ctacggaata cttgggttcg
    11341 cccaggaaat atccaatgaa ttccagtacc agatttcccg taaaatcctg aattggcgaa
    11401 atatccctaa acatctcctg cagcccttct tcaaggaacc acttataaga tgtttgctga
    11461 atttctatca gattgggcag atccagaacc tcgtcaatcc gtgcataggt gcggcgctcc
    11521 cgtgttcccg cgtcgatgac tttgaacatt caggcattca ctcctgtccc taggttttac
    11581 ccgctataga aataacaata ccttcctcaa aatacttacc taaaaagtaa acacagctaa
    11641 cggcactcgt aacatcttat tctttcccga tcgaaacaat aatatgcata aatatagcaa
    11701 agaaggcatt gttaagcaat ttacgatttt atcacaagtg tttacctatg tcaagaaaat
    11761 gaggaagatt tttccctagt ttcactggta ttgagaaatg ggaaaaaggc acccgcaaca
    11821 gggtgccttt taaagctata aaaacactta cttaagttcg acactggcac cagcttcttc
    11881 aagtttagct ttcaaagctt cagcatcttc tttgctgatt ttttctttaa ctgcttttgg
    11941 tgcactgtca actacctctt tagcttcttt taagccaaga ccggtaactt cgcgcacaac
    12001 tttaatggta gctacttttt tctcaccagc agcgagcaga attacatcaa attcagtttg
    12061 ttcctcaaca gcaggagcat caccggcaac aggcatagct gccatggcca caggtgcagc
    12121 tgcacttaca ccaaattctt cttcaaaagc tttaactaac tcggacagtt caagcactgt
    12181 catttcttta acgatatcaa gaacttcaac aactttagac atttatagtt cctccttata
    12241 attaaacaat tttttttggt attttaattt gcacttaagc ttcggcagct tttttttcac
    12301 gtacggcgtc aagcgtacca acaaatttac gcagcaaggc ttgggctgca ccagcaaaac
    12361 tagtcatcgg tgaagcgaaa gcaccggcaa catgagctaa caatacttca cgcgcaggca
    12421 gatctgctaa ggctttaatg cgactagcat caatcagccc gccttccagt aatccacctt
    12481 tgagctccag cttgtcgaac tccttggcat actttgataa aactttggca ggagcgaccg
    12541 ggtcttcaaa accaaaagca atagcagtag gtccttccag ataaaggtct aatccttcat
    12601 aacccaactc atgcgcagca atccgcacca gagtattttt ggcaacagtg tatttcacat
    12661 tttctgcccg cagggcgcgg cgcaaggtgg taatttgttg gacattcaaa ccccgataat
    12721 ctgtgataat cgctgtttta gcattactta aatcttcttt aacttcggca acaacttgcc
    12781 tctttgtttc cttacttata ctcatcgttc cacctccttt acgaacaata accaaaagct
    12841 tgcagtgttt agcactctat aaactacagc ggcagttttt aactacagta taaaatggcc
    12901 tccgtgcaga cacacggagg ccatcacggg ttgaaattaa caacacagtt gatgacattc
    12961 cgacctcggc aggcaataat ttaagtcctc gcggacacct gctgtctgca gtcaaaacta
    13021 acttagtatt tatacatgct cgggcgcatc gtatcatata cacccgtttt tggcaagatt
    13081 aattatttta ctttacccag ctgtgcggct ttttgcgtac ttatcggtac tcctggtccc
    13141 atagttgtag aaacagtaat ggaacgcaga tactggcctt tcgctgcagc tggcttaact
    13201 ttttgcaaag tatcgataat ggtgtagaag ttctctagca gcttctctac ggcgaacgat
    13261 actttgccaa tgggtacatg gataatactg tttttatcta cacgatactc cactttaccg
    13321 gctttaatct cattgatggc acgagcgaca tccatagtta ctgtaccggt tttgggattg
    13381 ggcatcaggc ctctcggacc taagatacga ccaagcttac cgacagtacc catcatatcg
    13441 ggtgtagcta ccgctacatc aaaatcaaac caaccctctt gtatcttggc caccaaatct
    13501 tcagcgccca caaaatctgc tcctgccatt tctgcttctt tggctttctc acctttggca
    13561 aaaacagcta cggtaacggc tttaccgata cctgcaggca gcaccacagc gccgcgaacc
    13621 tgctgatcag catgcttggg gttgacaccc agtctgacag ccagttcaac agtaccatca
    13681 aacttggtag ttgcggtttt ctgggccaaa gctagcgctt cttccggctc ataaggttgt
    13741 tcacgatcaa ttagctttct cgcgtctaaa tactttttac cgtgtttagc catttctttc
    13801 cctccttgtg gtgcgagcga ggcggcaaca cctctcccac gcaaccaata aattattttt
    13861 gaattgtaat acccatactg cgtgcagtac cttcaaccat ccgcattgct gcttctacat
    13921 cattagcatt aagatcttcc attttaagct ctgcaatttt acgaacttta tcccgtgata
    13981 atgtggcgac tttaacttta tttggttccc cagaagcagt atcaagaccg gctgcttttt
    14041 tcaacaagac agctgcaggc ggggttttag taataaagct aaaggaacgg tcttcataaa
    14101 tggtaatcac aacagggatt actaaaccag cttgttgagc agtacgctca ttaaactctt
    14161 tacaaaagcc catgatgtta acgccgtgct gaccaagagc aggacctacc ggaggcgcgg
    14221 gagtagcttt acccgcagga atttgcagtt taacaagacc ttttactttc tttgccatct
    14281 attacacctc ctatctttta ctatagcttc tctacctgaa aatattctag ctcaaccgga
    14341 gtttcccggc caaacataga aacaaggact ttaatcttac gcctgtctgg taaaacttct
    14401 tccactttgc caataaaatt agcaaacggt ccttcaacga cccgcacact ttcgccagtt
    14461 acaaaatcaa ttttgggttt aggctcgtcc atgcccatct gcttaagtat ctgggcgatc
    14521 tcgctttccg agaggggaat gggcttagag ccgggtccaa caaatccggt aactcctgga
    14581 gtgttacgaa caacatacca cgaatcatca gtaatagtca tttccactag cacatagccg
    14641 ggaaagacct tttttaacgt agactttcgc ttgccgtttt taatttcaaa ctctttttcc
    14701 atgggcacga ggacacgaaa gatcttatcc tgcatctcca tggactctac gcgtttttcc
    14761 agatttgttt ttactttgtt ttcataacct gcatatgtgt gcaccacata ccagttttta
    14821 ctcataggcc aggggaatac ccctacctcc ttactattgg agaatcagtt tcagcagctc
    14881 tgtaaatcct gcatcgagca gccaaaagaa aatcccaatg aaaaatacgg ttgtcagtac
    14941 cattgaagta tagactgtta gttcacggcg gttaggccag tgaacttttt tcaattcttg
    15001 tctgacttcc ttaaaatatt ttgtgaggcc tttgacattg gcagccactt aaccacatcc
    15061 ttgcaacaaa aatcaaatat tgcttacgct cgtctacgca gcaaacgttt tgcaaacgag
    15121 ttagacgcta ctttgtttct ttatgggcgg tatgtctttt acaaaacgga caatatttgt
    15181 ttagttcaat tcgatccgga gtggttcgct tgttcttgtt cgtaatataa ttccgccgtt
    15241 tacactcacc gcaagccata gtaattttca cgcgcattgc tcacaccccc taaacacgat
    15301 gttgggctct taaaatgcct gtaaactcaa gataaacccc aggatgcgcc tgagatttca
    15361 ctttttcaaa tttaccatac tttatagtgc gttgtcaaac tttttgtcat tagcgcagcg
    15421 ccacaaataa ctaatatgac tatttattat aaccggaaaa gtatttagca tacagccccg
    15481 ctagcagtga tcgctaacgg ggttgtatgc tcattgccca accatcgaga cacatcaagg
    15541 gtgggttgct ctgttagtgg aggaacagtt ttatgcgata atgctagtaa caacaccagc
    15601 gcccacagtg cggccgcctt cacggatcgc aaaacgtaga ccttcttcca aggcaatgga
    15661 agtaatcaac tcaattttca tttggatatt atcgccaggc atcaccattt ccacgccctc
    15721 gggtaaggta atcacacctg tgacgtcagt tgtgcgcagg taaaactgag gacgataacc
    15781 ttggaagaaa ggggtatgac gaccgccttc ttctttcttt aggacataaa cttcggccat
    15841 aaagttggtg tgaggcttaa tgctggcagg tttagcgagg acctgaccac gttcgataga
    15901 gtcacgatca ataccacgca gcagacagcc aatattgtca ccggctacag cttgatccat
    15961 aatcttgcgg aacatttcga cacctgttac gacagtttta cgtggttttt cagataaacc
    16021 aacaatttct acttcgtctt ggaccttaac tacaccgcga tcaacacgac cggtagctac
    16081 tgtaccacga ccagtaatgg tgaaaacatc ctctacaggc attaagaaag gcttatcaac
    16141 atcacgctcg ggatcgggaa tataa
//
LOCUS       DASRMS010000232        13790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_669919, whole genome shotgun sequence.
ACCESSION   DASRMS010000232 DASRMS010000000
VERSION     DASRMS010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 13790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13790
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669919"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            352..990
                     /locus_tag="VFC74_11020"
     CDS             352..990
                     /locus_tag="VFC74_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823746.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HZK25895.1"
                     /translation="MNELAKALRDLSEDKVNELVDEKLAQKVSPIEIINELNEGIVAV
                     GELYASGQYFLTELMFSGEIMQGLLSKLEPFIAVKSSEGTIVGTVVIGTVRGDIHDIG
                     KNIVVNLLRSHRFKVIDLGVDVPAEKFVEMVRTSGTKVVGLSALLNTTYPEMKNVVTA
                     LEKAGLRDQVKVIIGGTIVSEAVREFTGADAYATDAVKGIEYCKRVYQAAQV"
     gene            1105..1257
                     /gene="scfA"
                     /locus_tag="VFC74_11025"
     CDS             1105..1257
                     /gene="scfA"
                     /locus_tag="VFC74_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="six-cysteine ranthipeptide SCIFF"
                     /protein_id="HZK25896.1"
                     /translation="MEKKHIVTVNPEAAEAFSVHTGCGECPTSCQSACKTSCTVANQP
                     CIKEKN"
     gene            1444..2796
                     /gene="scfB"
                     /locus_tag="VFC74_11030"
     CDS             1444..2796
                     /gene="scfB"
                     /locus_tag="VFC74_11030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0031179 - peptide modification [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioether cross-link-forming SCIFF peptide
                     maturase"
                     /protein_id="HZK25897.1"
                     /translation="MVHTFKMHDLNLALDVESGALHILDDASYYAVEMLIKGAAPLEV
                     KQALAAAFGQAMAEQVAAEIADLQTQGLLFSEKRALDLTTDDAVKALCLNLAHECNMR
                     CTYCFASEGHYGGTRGLMPLDTAKEAVDFLLRASKERRHCEIDFFGGEPTLNFDVLKE
                     TVAYGREQAAKLDKVLKFTITTNALHLTEEMISFINDEQLSLVLSLDGREAVHDRFRV
                     LPSGAGSWRQVLANCQKIVAGRQDNNYYLRGTYTRHNLDFAEDVKEMVRHGFTSLSLE
                     PVVLAAEAEQALQQEDLPSLFAEYEKLVALLWDWEQEGKKIYFFHFELDLHQGPCAKK
                     RALGCGAGNAYLAVTPDGKIYPCHQFADQAEFCAGQVATGVDSDSLNRFQVVDSVEKE
                     ACRDCFARFFCGGGCHAAAWNINHDFLKPYALGCELHRKRVECGLYLQAKRSLAKMNG
                     "
     gene            2802..4142
                     /locus_tag="VFC74_11035"
     CDS             2802..4142
                     /locus_tag="VFC74_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HZK25898.1"
                     /translation="MDIFTLREEEELKQHGPLALRLRPKSLSEFAGQKHLVGVGKPLR
                     RLIESDVLSSLLFYGPPGTGKTTLAEIIAVKTNAAFVRVNAVASGVSELRKIMEAARM
                     RLAQDGKRTVLFIDEIHRFNKAQQDALLPAVEKGLVVLIGATTENPYFTVNAPLLSRM
                     RIFPFEPLLENDITELLLRALVVPEARLAAVNLTEEALEHLAIMANGDARSALNALEL
                     AAALAQADEQGQKIIDLQLAEEAVQRRAVVYDRDGDAHYDVISAFIKSVRGSDPDAAL
                     FWLARMLEAGEDPLFIARRIVILASEDIGNADPHGLLVAVAATQAVQMLGMPEGRIAL
                     AQATTYLAAAPKSNAAYLGIDEALALVKKSPGERVPVHLRDTSYSGAKKSGQGKKYLY
                     PHNYQGSFVVQNYLPEGMTRPKLYQPGENGYERSIKERLARWDAQRQRKGEKDD"
     gene            4135..4776
                     /locus_tag="VFC74_11040"
     CDS             4135..4776
                     /locus_tag="VFC74_11040"
                     /inference="COORDINATES: protein motif:HMM:NF013378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HZK25899.1"
                     /translation="MIDRFDTVAAPVRVKISVGACRFIASVARSDSEGKAKAFIEQIS
                     QEFSDATHNAYAYKIGLGDAAICRQSDAAEPAGTAGPPMLQTIENAGLTNVTVVGTRY
                     FGGVKLGIGGLVRAYRACAEAGLQAAARVTEVFYETVKIAISYELIGTVLREIASCNG
                     EVSDVKYGEQGARVLGTVPLSSLENLQIKLLEATRGKAELTLGDTNNSREKAE"
     gene            4781..5887
                     /gene="mnmA"
                     /locus_tag="VFC74_11045"
     CDS             4781..5887
                     /gene="mnmA"
                     /locus_tag="VFC74_11045"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018085646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HZK25900.1"
                     /translation="MTAKNKKRVVVAMSGGVDSSLAAALLTEQGYDVIGMTMRLWVAD
                     EFEDEAKQSSRGCCSLSSVEDARKVADKMGIPYYVVNFKEQFRQKVVDYFIDQYRQGR
                     TPNPCIACNRYMKFDLLLQRALDLEADYLATGHYARVSYDRNKQRYRLQKAKDAAKDQ
                     TYALYNLTQEQLAHTLFPLGGYLKSEVRQLAADYGLAVATKPDSQEICFIPDNDYKRF
                     LKEESELTAEPGPILDGAGNQLGTHQGLVNYTVGQRKGLGLAVGKPMFVVKLDLDRNQ
                     LIVGEDKDVYFSSLIAADCNYILLADLTAPLPVKVKIRYHAQETAGIVTPLTRGHVRV
                     DFAEPERAVTPGQSVVFYQGQDVLGGGIIQEAIR"
     gene            5902..7140
                     /locus_tag="VFC74_11050"
     CDS             5902..7140
                     /locus_tag="VFC74_11050"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HZK25901.1"
                     /translation="MKAEIIATGTELLLGQTVNTNARFLSEKLAEKGIDVYWQTVVGD
                     NAQRLETALRLALSRSDLILTSGGLGPTMDDLTKETVAKVLSLPLVVDADWNEQLTTM
                     FKLRGNVMTNNNLKQALLPSGAKLITNDNGTAPGVWLEHQDKTFVLLPGPPRELEPMF
                     TDKIMPLLPASGSSLVSRTLKIVGIGESSLEDKLAELIAAQTNPTIAPLAKLGEVHLR
                     LTAKAQTRAECQQLLDLTEAKLTERLGEAVYAKDEQTMATVVAELLEKSQLKLALAES
                     CTGGYLAHTLTNIAGSSKFFVAGLVTYSNAAKSKLLDIPLQFIEEHDAVSAEVGRAMA
                     SNVRRAAQSDLGIGITGIAGPTGGTAAKPVGLVYIALATPATVTCQRFNFFGKRENIK
                     ERAVISALNMLRLYLMQQLG"
     gene            7145..8560
                     /locus_tag="VFC74_11055"
     CDS             7145..8560
                     /locus_tag="VFC74_11055"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HZK25902.1"
                     /translation="MFRENRRQIFTLLLLAIIGMVAFVSLLGTFTTGMEALQVELGLQ
                     IFDRGYTQLEIPPLGRVRAQTHLPPLMLKVRLDNINLQQLQELMEHTDDEAYLANLQD
                     TARRKIEIFIARLLALAFIGGFAGPFFFGIRERKQLIAAGLIGIIVLGSLLAGAYFSY
                     QPLAFMNPEFEGILKAAPWLFGILEEALFSVQTLGEQLELVAVSINSLFQQVERLEPL
                     GTTEGDLKVVHVSDLHNNPAGIDFLRQVIDTFNVHMVIDTGDITDFGTEIEAALADPI
                     EEFGIPYIFVPGNHDSPGVIERMKTLANVTVLEEAQIEVLGLRIAGIVDPSAGDSGMV
                     VAVEPVLDEYARRLEKLIKKSGQSPHIVAAHHPRIASFFLPQIPVVLTGHLHRYDIRV
                     QSGSVMINAGTTGAAGIRGLQTGQETPYSLVLLHFGLRSDGELYLKAADTIHVYQLRS
                     GFSLERRLFGSTASEHDLEAD"
     gene            8745..9785
                     /gene="recA"
                     /locus_tag="VFC74_11060"
     CDS             8745..9785
                     /gene="recA"
                     /locus_tag="VFC74_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HZK25903.1"
                     /translation="MSKEKEKALEMAILQIEKQFGKGSVMKLGASAAQMNIAVISTGS
                     LAIDRALGVGGYPRGRIIEIYGPESSGKTTVALHAIAEAQKAGGYAAFIDAEHALDPK
                     YAKNLGVNTDEMLVSQPDTGEQALEISESLVRSGAIDILVIDSVAALVPKAEIEGEMG
                     DSHIGLQARLMSQAMRKLSGAISKSNTTAIFINQIREKVGVMFGNPEVTPGGRALKFY
                     ASVRLEVRRTETLKQGADAVGNRTRAKVVKNKVAPPFKQAEFDIIYGEGISREGDLLE
                     LGVEIDLIQKSGAWFSFGDVRLGQGRENARQFLKENQDIALHIERKIRETYNMPINAL
                     PEETETAKAEKK"
     gene            9782..10246
                     /locus_tag="VFC74_11065"
     CDS             9782..10246
                     /locus_tag="VFC74_11065"
                     /inference="COORDINATES: protein motif:HMM:NF014668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HZK25904.1"
                     /translation="MSSDPLKRARGLAYRLLARRARTVKEIRDNFKRKEIPAEVSTQI
                     IDELLAAGYLNDFAYAQQYIEYHVTNKQHGPLWLRASLQRAGIERELISRALSAVFLP
                     GCEEALAEQYLTKLCARADLSPQKAMRSLLSRGFTTQAARQAAAKVIDFQEF"
     gene            10637..12178
                     /gene="rny"
                     /locus_tag="VFC74_11070"
     CDS             10637..12178
                     /gene="rny"
                     /locus_tag="VFC74_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HZK25905.1"
                     /translation="MSTGYVVLIAVITAIVAAAAGYLFRKQFAERAIGSAEQAAKTIL
                     QEAEKQAQAAKREKIIEAKEEVHRMRRDLDQESRERREEFKRSERRLLQKEEALDRKI
                     AQNERKEEEVKQKEAEVTSIQEKITEALQQQLSELERIAVMTTDEAKEILLSRVRDEV
                     KHEMALMIKEIEGQAKESAEKKAREIITGAIQKFAADHVSETTVSVVSLPNDEMKGRI
                     IGREGRNIRALETMTGIDLIIDDTPEAVILSGFDPIRREIARIALEKLVADGRIHPAR
                     IEEMVEKARQEVDVQIREVGESATFEAAVHGLHPELVKLLGRLRYRTSYGQNVLRHSI
                     EVAHLAGAMAAELGLDIQFAKRAGLLHDIGKAVDHEVEGPHVTIGADLARKYKESRQV
                     INAIAAHHGDEEYLTLEAVLVQAADAISAARPGARRETLEAYIKRLEKLEAIADSFEG
                     VEKAYAIQAGREIRIMVKPDKVDDTSSVTVAREIVKRIEEELEYPGQIKVTVIRETRA
                     VDYAK"
     gene            12378..12896
                     /locus_tag="VFC74_11075"
     CDS             12378..12896
                     /locus_tag="VFC74_11075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25906.1"
                     /translation="MGQIKQQSKQDAYMTNLMISLLMRFPEIMSIRFDMPQDKAKFTF
                     ILQGKPEKEQYKGFTDLLKESLAAFQDLTGEVLQVKTTMQRSGKVSLLVLSSSTKSLS
                     LEGIHLLCGLVCSSFAESVIRDADTSVDLQDDELLRQEEIIEYLLSHNTGTKKDNLLA
                     FREAGKVFVYDK"
     gene            13004..13789
                     /locus_tag="VFC74_11080"
     CDS             13004..13789
                     /locus_tag="VFC74_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HZK25907.1"
                     /translation="MRFLLIGDVVGRPGRHCVRDLLPRIIENYKIDFIIANGENLAAG
                     TGFNERTAMELFSYGVDVLTMGNHVWDKKEALHYIEKEKRIVRPVNYPAGTPGQGYAL
                     YHCKGQAIGVINASGRVFMPTLDCPFRAVDKAVAYLQQKTNIILIDFHAEATSEKIAL
                     GRYFDGRVSAVVGTHTHVQTADERILTKGTAYITDLGMTGALESVLGVEPELVLRKFL
                     TQMPVRFEVAKDGPSQFNGVIVDVSEETGKACEISRFTDLHEF"
BASE COUNT         4027 a         2667 c         3368 g         3728 t
ORIGIN      
        1 atataatatt gatgaaaatg tagttagtta aattattttt tactataaat gcatatacta
       61 atgttgcaat ttgtctctta gctgctgcta gacgttcagc taacgttcag tacggactgc
      121 ttctctgaac gttttaagat aaataatacg cattagttgg aggtgagtta cttaaaaacg
      181 catgacattc tacgttagtg taagctgtta aaaagtgcag tttttaagag taacatatga
      241 tgtgctatca ttttcaacaa agattcacaa gggaacttat acatttaaat gtgtgaagtc
      301 tgttttaaga atggtgaaga tgcactcgca gaaaggggta agcattcaat catgaacgaa
      361 ttagctaaag cattacgcga tttaagtgag gacaaggtga acgagctggt agatgaaaaa
      421 ctagcacaaa aagtttcacc gattgagatt attaatgagt taaacgaagg cattgtggcc
      481 gttggtgaat tgtatgcttc aggtcaatat tttcttaccg aactgatgtt ttctggtgaa
      541 ataatgcaag gattactgtc taagctggaa ccttttattg ccgtcaaaag ttccgaaggc
      601 acaattgttg gcactgtcgt cattggcaca gttcgtggtg atattcatga catcggcaaa
      661 aatattgttg ttaacctact ccgtagtcat cggtttaaag taattgatct aggcgtagat
      721 gtaccggcag aaaagtttgt agaaatggta cggacaagtg gcacgaaggt agtaggttta
      781 agtgctttat tgaatacaac atatccagaa atgaaaaatg tggttacggc cttagagaag
      841 gctggcttaa gagatcaagt taaagtaatt attggcggca ctattgtcag cgaagctgtg
      901 cgtgaattta ccggtgcaga cgcctatgct actgatgctg taaaaggtat tgagtactgt
      961 aagcgtgttt atcaagctgc acaagtatag agacaactaa cggtgctcaa gcaaacgctt
     1021 agcaccgttt ctgtttgcat atctttaccg tttgcctcat ttgtgctata attaggctgt
     1081 aatctggaga aggaggcaaa tcctatggaa aagaaacata ttgttactgt aaatcctgag
     1141 gctgcagaag ctttcagcgt acatactggt tgtggtgagt gtcccacatc ctgccaatcg
     1201 gcttgtaaaa cttcatgtac tgtcgctaac caaccttgta tcaaagagaa aaactaatca
     1261 tcaggataac tacttaatga agtaagcgtg atagcgtgct ataagcaatt aatgcacgct
     1321 tttcttgtta aatgaacatg aactaagaaa gtcttaatcg cggaattacc cttttattga
     1381 gcaggtcggc gcgacaaaga accgtcccct gtcacacttg agatgaaatg aggaagagag
     1441 ccaatggttc atacatttaa aatgcatgat ttaaatttag cgctagatgt ggaaagtggc
     1501 gctttacata ttttagacga tgcttcttat tacgccgtgg agatgctgat aaagggtgcc
     1561 gcccctttag aagtaaaaca agcactggcg gcagcttttg gccaagcaat ggctgagcag
     1621 gttgcggcag aaatagctga cctacaaact caaggattat tgttcagtga aaaaagagca
     1681 cttgatttaa ctacggatga tgccgtcaaa gcactttgtt tgaatttagc ccatgaatgt
     1741 aatatgcgct gtacgtactg ttttgctagt gaaggacact atggcggcac gagaggcctc
     1801 atgccgcttg acactgctaa agaagctgtt gacttcttgc tgcgggccag taaagaaaga
     1861 cgccattgtg aaattgactt tttcggcggc gaaccgactc taaattttga cgttttaaaa
     1921 gagactgttg cttatggacg tgagcaggca gccaaactag acaaagtatt aaaattcact
     1981 attaccacca acgccttaca tttaactgaa gaaatgatca gttttatcaa tgacgaacaa
     2041 ttgagtctag ttttgtcgct agatggccga gaagctgtac atgacagatt tcgtgtgctg
     2101 cccagcgggg ctggcagttg gcggcaagta ttggctaatt gccagaaaat agttgctggg
     2161 cggcaggaca ataactatta cctgcgcggc acttatacac gacataacct tgattttgct
     2221 gaagatgtca aggaaatggt gcgtcatggt tttacttctc tttcgctaga accagttgtt
     2281 ttggcggcgg aagcagaaca ggctttacag caagaggatt tgccaagctt atttgcggaa
     2341 tatgaaaaat tggttgctct cttatgggac tgggaacaag agggcaagaa aatatacttt
     2401 ttccattttg agttagattt acaccaaggc ccctgtgcca aaaaacgagc tttaggttgt
     2461 ggagcaggga atgcttattt ggctgtgacg ccggatggga aaatatatcc ttgccaccag
     2521 tttgctgatc aagcagagtt ttgtgctgga caagtggcaa cgggtgtcga tagcgacagt
     2581 ttaaaccgct ttcaagtggt agactcggtg gaaaaagaag catgccgaga ttgttttgcc
     2641 cgctttttct gtggcggtgg ttgtcatgcg gcggcgtgga atattaatca tgattttcta
     2701 aagccatatg ctttaggctg tgaactacat cgaaaaagag tggaatgtgg tctttattta
     2761 caggccaaac gcagcttggc caaaatgaat gggtgattat aatggatatc tttaccctgc
     2821 gtgaggaaga ggaattgaaa caacatggtc ctttagcttt acgcttgcgt cccaaaagcc
     2881 tttcagagtt tgcaggacaa aaacatctgg ttggtgtggg taagccttta cggcgtttaa
     2941 ttgaaagtga tgttttatca tctctgcttt tttacggacc accaggaacc ggcaaaacca
     3001 cattagctga aataattgct gtaaaaacaa atgcagcctt tgtacgtgtt aatgctgtgg
     3061 cctccggtgt gagtgaatta cgtaaaatca tggaggctgc gcggatgcgt ttagcccagg
     3121 atggcaaacg aactgtgctt tttattgatg aaatccaccg ttttaataaa gcgcagcagg
     3181 atgctttact gccggctgta gaaaaaggat tggttgtttt gattggtgca actactgaaa
     3241 acccctattt tacagtaaat gcacctctgc tttcccgtat gcgtattttc ccttttgaac
     3301 ctttgcttga aaatgatatc accgaactat tactgcgcgc actcgtcgtg cccgaagcac
     3361 gtctcgctgc tgtcaacttg acagaagagg ctctggagca tctggcgatt atggcaaatg
     3421 gtgatgctcg ctcggcttta aatgctttag aattagccgc ggctttagca caggcagatg
     3481 agcaggggca aaaaataatt gatttgcagt tagcagaaga ggctgtgcag cgtcgtgccg
     3541 ttgtctatga ccgggacggc gacgcacatt atgatgtcat ttctgccttt atcaaatctg
     3601 tgcgcggttc agatcctgac gctgcgcttt tttggctggc gcgcatgctg gaagctggtg
     3661 aagatccgct atttatcgcc cggcgtattg ttattctggc ctctgaagat atcggtaatg
     3721 ctgatcccca tggcctgtta gtagcggtgg cggccacaca agctgtgcag atgctgggaa
     3781 tgccggaagg gcgtatcgct ttggcgcaag caactactta tctggcggcg gcaccaaaaa
     3841 gtaatgctgc ctatcttggc attgatgaag ctttagcctt ggttaaaaag agccccgggg
     3901 aaagggtgcc ggtacattta cgtgatacta gctacagtgg agcaaaaaaa tctggtcagg
     3961 gcaaaaagta tctctaccca cataattatc agggtagttt tgtagtgcaa aattacctgc
     4021 cggagggtat gactaggcct aaattatatc aacctggaga gaatggctat gaacgttcca
     4081 ttaaagaacg tctggcgcgt tgggatgcac aacggcagag aaaaggtgaa aaggatgatt
     4141 gatcgttttg atacagtggc ggcacctgtg cgcgtaaaaa tatcagtagg tgcgtgtcgc
     4201 tttattgcca gtgtcgcacg cagtgatagt gaaggaaaag ctaaagcttt tattgaacaa
     4261 ataagtcagg aatttagtga cgccactcac aatgcctatg cttataaaat tggactgggt
     4321 gatgcagcta tttgtcgtca gagtgatgcg gctgaaccgg cgggtacggc gggccctccc
     4381 atgctgcaaa cgatcgaaaa tgctggctta actaatgtta ctgtagtggg gacacgctat
     4441 ttcggtggtg tgaagctagg cattggcggt ttagtgcgtg cttaccgggc ctgtgccgaa
     4501 gctggtttac aggcagccgc aagggttact gaagtttttt atgaaactgt taaaatcgcc
     4561 atctcttatg agttaattgg cacagtgctg cgggaaattg cctcctgtaa tggtgaagta
     4621 agtgatgtta aatatgggga gcaaggagcg agggtactgg gtactgtacc tttatcgtcc
     4681 ttggaaaact tgcaaattaa acttcttgag gctacccggg gtaaagcaga actcactttg
     4741 ggtgatacta acaatagtcg agaaaaagcg gagtgatagc atgacggcaa aaaataaaaa
     4801 acgtgtcgtg gtagcgatga gcggcggtgt tgatagttca ctggccgctg ccttgctgac
     4861 tgagcagggt tatgatgtga ttggtatgac catgcggtta tgggtcgccg atgaatttga
     4921 ggatgaagcc aaacaatcca gccgcggctg ttgttcgctc tcctcagtag aagatgcccg
     4981 caaggtagcc gataaaatgg gtatacctta ttatgtggta aattttaagg agcagtttcg
     5041 gcaaaaagtt gttgattatt ttatagatca atatcggcag ggacgcaccc ctaatccctg
     5101 cattgcctgt aatcgctaca tgaaatttga tctgcttttg cagcgcgctt tagatttaga
     5161 agcagactat ctcgccacag gccattatgc acgggtaagc tatgaccgaa acaaacaacg
     5221 ctatcgccta caaaaagcta aagatgctgc caaagatcaa acttatgccc tctataattt
     5281 gactcaggag cagttagccc acacgctatt tcctttaggt ggctatttaa aaagcgaagt
     5341 gcgccaactg gcagctgact atggtttagc agttgcgact aaacctgata gccaggaaat
     5401 ttgctttatt cccgataatg attataagcg tttccttaag gaagagagtg agctgacggc
     5461 tgagccagga ccaattttag atggcgccgg caaccagctg ggaactcacc aaggtttggt
     5521 gaattacacc gtggggcagc gcaagggttt aggccttgcc gtgggtaaac caatgtttgt
     5581 ggtgaaatta gatcttgatc gtaatcaact aattgtggga gaagataaag atgtttattt
     5641 tagctccctg attgcagcag actgcaatta tattttgctt gcagacttaa cggcaccgct
     5701 gccagtaaaa gttaaaatac gctatcacgc ccaggaaaca gcggggatag taacaccact
     5761 gaccagaggt catgtacgtg tagattttgc cgaaccagaa cgtgcagtca caccaggcca
     5821 atccgtcgtc ttctaccaag gtcaggatgt actgggcggc ggtattattc aagaagcgat
     5881 ccgctagaga gaagggaagt tatgaaggca gaaataattg ctacaggcac agaattattg
     5941 ttagggcaga cagttaatac caacgctcgc ttcttatcgg aaaagctggc ggaaaaaggc
     6001 atagatgttt actggcagac tgtggtcggc gataatgccc aacggttaga aacagcctta
     6061 cgtttagccc tgtcacgttc tgacttaatt cttacctcag gcggcctagg ccctaccatg
     6121 gatgatctca ctaaagaaac tgttgctaaa gttttatcac tgcccttggt agtagatgct
     6181 gactggaatg aacaattaac cacgatgttt aaactgcgcg gcaatgtgat gactaacaat
     6241 aatcttaagc aggcactgct gcctagcggt gccaagttaa ttaccaatga caacggtaca
     6301 gcccctggag tatggctgga gcaccaagac aaaacttttg tgctcttacc aggaccaccc
     6361 cgtgaattag aaccaatgtt tacagataaa attatgccat tattgccggc tagcggcagt
     6421 agtttagttt cccggacctt aaaaatagta ggtatcggcg aatcaagctt ggaggacaag
     6481 ctagctgagc ttattgcggc acaaaccaat cccaccattg cccccttggc aaaattagga
     6541 gaggtgcatc tacgcttaac agctaaagct caaaccagag cagaatgcca acagttatta
     6601 gatctgactg aagcaaaact aactgagcgg ctcggtgagg cagtttatgc caaagacgag
     6661 cagacaatgg caactgtggt ggcggaactt ttagaaaaaa gccaactaaa gctggccctg
     6721 gcggaatcat gcacaggggg ttatttagca cataccctaa ccaatattgc cggcagttct
     6781 aaattctttg tggcaggact tgttacttat agtaatgctg ccaagagtaa acttttagac
     6841 atccctctgc agttcataga agaacacgat gctgtttctg cagaagtagg acgagcgatg
     6901 gccagtaatg tcagacgagc agcacaaagt gatttaggta ttggtattac cgggattgcc
     6961 gggcctacag gtggaacagc tgccaaacca gttggacttg tttatatagc tttggccaca
     7021 ccagccacag taacttgcca aaggtttaat tttttcggga aacgggagaa tataaaagaa
     7081 cgggcggtca tcagtgcatt aaatatgctg cgtctgtatc ttatgcaaca acttgggtga
     7141 taggttgttt agagaaaatc gtcgtcaaat ttttactttg cttctgttag caattatcgg
     7201 tatggtagcc ttcgttagtt tgttaggtac ttttactact ggtatggagg cactgcaagt
     7261 agaactaggc ttgcagattt tcgaccgcgg ctatacacaa ttagagatac cccccttggg
     7321 tcgtgtgcgc gcccaaactc atctgccgcc gttaatgcta aaagtacgtc tagataatat
     7381 taatttacaa caattacagg aattaatgga gcacactgat gacgaggcat atttagctaa
     7441 tctgcaggac acagctcgtc gtaaaattga aatatttatt gctagattgc tggcgctggc
     7501 ctttatcggc ggcttcgctg gtcctttttt tttcggcata cgtgagcgga agcaattaat
     7561 cgcagctggt ttgattggta ttattgtttt aggcagttta ctggctggag cctatttttc
     7621 ttatcaacca ttagctttta tgaatccaga atttgaaggt atcctcaaag cagcaccttg
     7681 gttatttggt attctggagg aagccttatt tagtgtgcaa actttaggtg agcaattaga
     7741 actagttgct gtcagtataa actcgctctt tcagcaagta gaacgtctag agccgctcgg
     7801 cacgactgaa ggcgacctta aagtagttca tgtctctgac ctccacaata accctgccgg
     7861 cattgatttt ctgcgtcagg tgattgatac tttcaatgta catatggtca ttgacacggg
     7921 cgatatcact gattttggta cagaaataga ggccgcctta gctgacccca ttgaggaatt
     7981 cggcattccc tatatctttg tgcctggaaa ccatgattca cctggtgtta ttgagcgcat
     8041 gaaaactctt gctaatgtta cagtattaga agaagcacag atagaagtac ttggtttacg
     8101 tattgccggc attgttgacc cttctgccgg ggacagtggg atggtagtgg ctgttgaacc
     8161 tgtacttgac gaatatgccc gccgtctgga aaagctgatt aagaaaagtg ggcagtctcc
     8221 ccatatcgtc gccgcccatc atcctcgcat tgcttccttt tttctcccgc agatccccgt
     8281 tgttttaacg gggcatttac atcgctatga catcagggtg cagagcggct cagtaatgat
     8341 taatgccggc accaccggtg ctgcgggcat acgcggtcta caaaccgggc aggagacccc
     8401 ttattcttta gtcttgttac actttggctt acggtctgat ggcgaattat atttaaaagc
     8461 tgcagataca atccatgtct atcaactacg cagcggtttt tcactcgaaa gacgcctttt
     8521 cggcagcacc gccagtgagc atgatttaga agcggattaa ccagagcttg agcggtaaga
     8581 agggcgtaca gtaatgagat agttcttaaa cctaggaagt gcagtaatga gcgtgcaaca
     8641 attacaaaaa gtatcataaa tatttttttt atagcaggaa ttgttgttta cacggagaat
     8701 ttaacattta aggcagggga acaaacgttt ggtaggaggt ttatatgtca aaggaaaaag
     8761 aaaaagcctt agaaatggca atattgcaga ttgaaaaaca gttcggtaaa ggttcagtga
     8821 tgaagcttgg tgcttccgct gcacaaatga atatagctgt aatttcaaca ggttcactag
     8881 ctattgacag ggctcttggt gtaggcggct atccacgtgg ccggatcatc gaaatatatg
     8941 ggcctgagtc ttctggtaag actactgtag ctttacatgc tatcgctgaa gcacaaaagg
     9001 ctggtggtta cgcagctttt attgatgctg agcatgcttt ggatcccaag tatgcaaaaa
     9061 atttaggtgt taatacggat gaaatgctgg tatcacagcc ggacactggt gaacaagctt
     9121 tagaaatttc tgaatcactt gtgcgcagcg gcgccattga tatactggta atcgattcgg
     9181 tggcagcact agtccccaaa gcagaaatcg aaggagaaat gggtgactct catattggtc
     9241 tgcaggcacg tttaatgtcc caagcgatga ggaaactttc cggtgctatt tcaaaatcaa
     9301 atactactgc tatttttatt aaccaaataa gggaaaaggt tggcgtcatg tttggcaacc
     9361 cggaagtaac tccaggtgga cgtgctctta aattttatgc ttccgtgcgt cttgaagtac
     9421 gccgtactga aacactcaaa cagggtgctg atgctgtagg caaccgtacg cgggctaaag
     9481 tggttaaaaa taaagtggcc ccacctttca aacaggcaga atttgacatt atatacggag
     9541 aaggtatttc acgtgaaggt gatctgcttg aactaggcgt ggaaattgat ctgatacaaa
     9601 aaagtggtgc ttggttttct tttggcgatg tgcgtttagg gcaaggccga gaaaacgcgc
     9661 gtcaattttt aaaggaaaat caagatattg ccctgcatat tgagcgtaaa attcgcgaaa
     9721 cttacaacat gcccatcaac gctttgcctg aagaaactga aactgctaaa gctgagaaaa
     9781 aatgagtagt gaccctctca aaagagcccg aggtcttgct tatcgcctgt tagcaagacg
     9841 tgcacgtaca gtaaaagaga tacgcgataa ttttaagcgt aaagaaatac cagcagaagt
     9901 gtcaacgcaa ataattgatg aattattagc tgctggctat cttaatgatt ttgcctatgc
     9961 gcagcagtat attgagtatc atgtcacaaa taaacagcat ggaccactgt ggttgcgagc
    10021 ttcactacaa cgtgcaggga ttgagcgtga gctaattagt cgtgcgctgt ctgctgtttt
    10081 tctgccgggc tgcgaagagg cactagcaga gcagtattta acaaagcttt gtgcgagagc
    10141 tgatctctca ccgcaaaaag ctatgcgcag cttgctctca cgcggcttta cgacgcaggc
    10201 agcgcgccaa gcagcagcaa aagtcatcga ctttcaggaa ttttaagtgt cacaactgtt
    10261 tgctcctagg agttagcagc gataaaattt ataaaaagct tgcaaacaga atttaaaagt
    10321 tgcaagtttt ttttttgagg aaaaatcttg tcttgtactt aatgcacaaa aacagtaaat
    10381 aattagcttt ccttgacata atgccctaaa acagataaaa tttaagggag aaaaagtaat
    10441 cgtgtgcgtc tcctgctaat tgggcgtttg gcatattgcc tgaacatata tatcttctac
    10501 tggtaaaaaa tcataatttc aatctgattc cagttaagat cagtatgcgc ttctctatag
    10561 ctgtgtgacg gcatagctat ttttattttt ctaaacaact atcttaatta aaactaacta
    10621 taggaggtga aatgaattga gtactggtta tgtagttctt attgcagtaa ttacggcaat
    10681 tgtggctgca gctgcaggat atttatttcg caaacagttt gctgagagag cgataggatc
    10741 agcagagcaa gcagcaaaaa ctattttgca ggaagctgag aagcaggcac aagccgcaaa
    10801 acgagaaaaa attattgaag caaaagaaga agtgcacaga atgcgcagag atctggacca
    10861 ggaaagccgt gaacgtcgtg aggaattcaa acggagtgaa cgtcggctgt tacaaaaaga
    10921 agaggctcta gatagaaaaa ttgcgcaaaa tgaaagaaaa gaagaggaag ttaaacaaaa
    10981 agaagcagag gtcaccagca ttcaagagaa aataacggaa gcgttacagc agcaattatc
    11041 tgagttggaa cgcattgctg tgatgacaac cgatgaagca aaagaaatat tactttcacg
    11101 cgtacgcgat gaagtgaagc atgagatggc cttaatgatt aaggagattg aaggccaagc
    11161 aaaagagagt gctgaaaaaa aagcacgaga gattatcact ggtgctattc aaaagtttgc
    11221 tgctgatcat gtgtcagaaa caactgtgtc tgtagtttcg ttgcccaatg atgaaatgaa
    11281 gggccgaatt attggtcgtg aaggccgcaa tattcgggca cttgaaacca tgacaggcat
    11341 agatttgatt attgatgata ctccggaagc agtgattctc tccggatttg accccattcg
    11401 tcgtgaaatt gcgcgcatcg ctttagaaaa attggttgcg gacggccgca tccatcctgc
    11461 tcgcattgaa gaaatggtag aaaaagcgcg gcaggaagta gacgtccaaa ttcgtgaagt
    11521 aggagagtcg gctacatttg aagccgccgt gcacggccta caccctgaac tagtcaagtt
    11581 acttggccgc ttgcgttatc gtacaagcta tgggcagaat gtgttgagac actccataga
    11641 agtagcccat ttagccggcg ctatggcggc agaactaggt ttggatatcc aatttgctaa
    11701 acgcgctggt ttactgcacg atatcggtaa agctgtagac catgaagtag aaggccccca
    11761 cgtgacaatc ggtgcagatc tggcacgcaa atataaagaa tcccgtcagg tcatcaatgc
    11821 cattgccgct caccatggcg atgaagagta tttaacactc gaagctgtac ttgttcaagc
    11881 tgctgatgct atttctgcag ctcgaccagg agcacgtcgg gaaacactgg aagcctacat
    11941 taaacgcctg gaaaaactag aagcaattgc agattcgttt gaaggcgtgg aaaaagccta
    12001 cgctattcaa gcaggacgtg aaattcgcat tatggtaaaa ccggataaag tcgatgatac
    12061 ctctagtgtt accgtagcac gcgaaatagt aaagcgcatt gaagaagaat tagagtatcc
    12121 cggtcagatt aaagtaacgg tgattcggga aaccagagct gtagattacg ctaaatagcc
    12181 ttatttgatc actaaagaca gaatggctta agcagccatt ctgtcttttt tacccatagt
    12241 tgcctggtgt tgaacatgca ggtggtagat atccataaat tgcagcttaa catttagcta
    12301 agacctgcag gattttacag ggcttctatg gaataacgaa gagagtatgg agatgcgacc
    12361 gggggttaga caaaaaaatg ggacaaataa agcaacaaag taaacaagat gcctacatga
    12421 ccaatttaat gatttcttta ttaatgcgct ttcctgagat tatgtctatt cgctttgata
    12481 tgccacaaga caaagcaaaa tttaccttta tactgcaagg gaaacccgaa aaagaacaat
    12541 acaaggggtt tactgatttg ttaaaggaat cattggcagc tttccaggat ttgacgggtg
    12601 aagttctgca agtgaaaaca acaatgcagc gttctggcaa agtaagttta cttgttttga
    12661 gttctagtac caaaagttta tctttagaag gtatacatct gctgtgtggt ttggtctgca
    12721 gttcgtttgc ggaatcagta atccgtgatg ctgacaccag cgtcgacctg caggatgatg
    12781 agctattacg ccaggaagaa attattgaat atttattaag tcataacacc ggaacaaaaa
    12841 aagacaactt actagccttt cgtgaggctg gtaaggtttt cgtctatgac aagtaaaaat
    12901 aaataaaaac attcactcat taggctgtgc acacagcctt ttatgtataa gtgaagggaa
    12961 aaaaaggtac actttccagt atggcgtgaa atgaggtgtc agaatgcgtt ttttattaat
    13021 tggtgacgtt gttggtagac ccggccgtca ctgcgtcaga gatttattgc caagaattat
    13081 cgaaaattat aagatagatt ttattatagc taatggtgag aatctggcag caggaacagg
    13141 ctttaatgaa agaacagcca tggaactatt tagctatggt gttgacgtgc tcacgatggg
    13201 caatcatgtg tgggataaga aagaagcttt acactatatc gagaaggaaa agcgtattgt
    13261 acgtcctgta aattacccag caggcacacc tggccaagga tatgcactct atcactgtaa
    13321 aggtcaagcc attggtgtga tcaatgccag tggacgtgtg ttcatgccta ctcttgattg
    13381 tccttttcgc gctgtggaca aagctgtggc ctatctacag caaaaaacaa atattatctt
    13441 aatagatttc catgctgagg ctacctcaga aaaaatcgct ctgggacgtt attttgatgg
    13501 acgtgtttca gcggttgtgg gaacccatac gcatgtacaa actgctgatg aacgaatatt
    13561 aacgaaggga actgcctata tcactgacct gggcatgaca ggagcgctag aatcagttct
    13621 tggcgtggaa ccagagcttg ttttgcgtaa atttctcact cagatgcctg ttcgttttga
    13681 ggtggccaag gatgggccca gtcagtttaa tggtgtgatt gtggatgtta gcgaagaaac
    13741 agggaaggcc tgtgagatta gcagatttac agacttgcac gaattttaag
//
LOCUS       DASRMS010000233         1092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_937129, whole genome shotgun sequence.
ACCESSION   DASRMS010000233 DASRMS010000000
VERSION     DASRMS010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_937129"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1027)
                     /gene="trpD"
                     /locus_tag="VFC74_11085"
     CDS             complement(<1..1027)
                     /gene="trpD"
                     /locus_tag="VFC74_11085"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HZK25908.1"
                     /translation="MKISYQEALTRLIEHREIFEDEMVDLMRDLMQGQMPPEIAAAFL
                     MGLRVKKETVDEITAAATVMREFSQTVTLKKPEEALDMCGTGGDGSNTFNISTTAMFV
                     AAAAGVPVAKHGNRSASSPAGSADGLEALGAFVETPPERVAESLETTGIGFMFAPFHH
                     SSMKNIAAVRKLLGVRTMFNILGPLTNPANAGNQLMGVFHPDLVGIQVRVLKQLGANH
                     VLIVHGLDGMDEATLGATTLVGELKDGKVTEYEIHPEDFGMQMVSNRSYRVTNKEESR
                     DIILSVLKNEASPALDVVILNAGLAIYAGNKADSIKEGIEIARELLASGAAFNKTQQF
                     CEFSQTLR"
BASE COUNT          278 a          256 c          251 g          307 t
ORIGIN      
        1 atcttaaggt ttgactaaat tcgcagaatt gctgcgtttt gttgaaggct gcaccgctag
       61 ctaaaagctc ccgggcaatt tcaatgccct ctttaatgct atctgcctta ttccctgcat
      121 aaatagccaa gccggcattt agaataacta catcaagagc cggacttgct tcatttttta
      181 agacgctaag aataatatct ctggattctt ccttgttagt cactcgatag ctacggttag
      241 acaccatttg catgccgaag tcctcagggt gaatttcgta ctcagtcacc ttgccgtctt
      301 taagctcacc aacaagtgtg gtagcgccga gagtagcctc gtccataccg tctagtccat
      361 ggacgatgag tacatggttg gcaccaagct gtttcaatac gcgtacttgt atgcctacta
      421 agtcagggtg aaatacgccc attaattggt tgccggcatt agcagggttg gttagtgggc
      481 caagaatgtt aaacatcgtc cggacgccaa ggagtttgcg tactgcagcg atgtttttca
      541 tactgctatg gtgaaaaggg gcaaacataa aaccgatgcc cgttgtttct aaactttcag
      601 caacgcgttc aggcggtgtt tctacaaaag cccctagagc ttctaagcca tcagcactgc
      661 ctgcaggact agaggcactg cggttaccat gtttggctac cggtacaccg gctgcagcag
      721 ctacgaacat agcagtagta gaaatattaa aggtattgct accgtcacca ccggtgccgc
      781 acatatccaa agcttcttcg ggttttttta aggtgactgt ctgactgaat tcacgcatga
      841 ctgtggcagc tgcggtgatt tcatcgacgg tttctttttt aacgcgtaga cccattaaaa
      901 aagccgcggc gatttcaggt ggcatctggc cctgcatgag gtcgcgcatg aggtcaacca
      961 tctcgtcttc gaatatttct cgatgttcga taagacgtgt aagggcttct tgataagaaa
     1021 ttttcatcag atttactccg cacggaaacc aagtttaaga gaaataaaac ctggttgtac
     1081 cggcatttat tt
//
LOCUS       DASRMS010000234         1538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Oscillospiraceae bacterium isolate SMAG_U10222
            k141_670984, whole genome shotgun sequence.
ACCESSION   DASRMS010000234 DASRMS010000000
VERSION     DASRMS010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37963748
            Sequence Read Archive: SRR5678806
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Oscillospiraceae bacterium (soil metagenome)
  ORGANISM  Oscillospiraceae bacterium
            Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae.
REFERENCE   1  (bases 1 to 1538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/09/2024 21:55:06
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,218
            CDSs (total)                      :: 2,163
            Genes (coding)                    :: 2,153
            CDSs (with protein)               :: 2,153
            Genes (RNA)                       :: 55
            rRNAs                             :: 1, 3, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 3, 1 (16S, 23S)
            tRNAs                             :: 46
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 11
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 11
            Pseudo Genes (frameshifted)       :: 3 of 11
            Pseudo Genes (incomplete)         :: 8 of 11
            Pseudo Genes (internal stop)      :: 1 of 11
            Pseudo Genes (multiple problems)  :: 2 of 11
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1538
                     /organism="Oscillospiraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670984"
                     /isolate="SMAG_U10222"
                     /isolation_source="soil"
                     /db_xref="taxon:2485925"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="43 N 92.5 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(536..>1538)
                     /locus_tag="VFC74_11090"
     CDS             complement(536..>1538)
                     /locus_tag="VFC74_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HZK25909.1"
                     /translation="IQAKWLLEGTLPRAALPRWNACQRYLIDRLKPAGADVGAKDASR
                     VLRLVETVNSKSNEICRVVHVENGADGQPVRYNFEYLAEILLPVARWDIEKQKQERDE
                     RQKRKQLKLLDGYKVSSNLRGFSGRQLAWHRLEDLRTLATLRGGVSEGDRMRHLFWRL
                     NFLLLSGATNSSLMYHEAATLAREVDSGWGYNSKELMTLYSKAKQYEAGEKVSFGGKD
                     FAPLYTPRNDTLISLFEITDAEQRELRTIISKGMALERDKDRKQTARRAAGAVDRETY
                     IEAASDKQAEAQTLRAQGLSVRAIAAQMGISKTAAGRYIQSENIKCPKSVRITNGEA"
BASE COUNT          405 a          420 c          315 g          398 t
ORIGIN      
        1 atagaattat cttttgcaag caagcaaaga gatcaaatga gtaatacccg cgaactttag
       61 atggccctgg aaggccgtac actcaccgcc aaaacgcgaa ccctagaaag ccatccttta
      121 gcctgcaaaa cagctaaggc aagcaaacca cgcagccagt taatcagtta tgtgtgttga
      181 accaaccgac aactagaacg agcactcact ttcgctccta gtagcggata tgaatatcct
      241 tttaaaagac taagagctaa aaacaaaaca ccgtaggctt cgccattagt aatacggacg
      301 gacttgggac acttacattc gcaacctgaa ttgcgcactt taccctagtg aatacgttta
      361 tctgaccagc taaagctctc cacaaatccg tacacgcaac gaaacaaggc aacccctaca
      421 acactagcca caccttttta aactattaca agacaaacac acagcctgtt aaccagttat
      481 atatgtttga cctaaccaac gctgaagcaa aaacaaagag tactaggcct acggcttagg
      541 cttcgccatt agtaatacgg acggacttgg gacacttaat gttttcagac tgaatatacc
      601 gccctgctgc tgttttacta atgcccattt gtgccgctat agcccgtaca ctcaagcctt
      661 gcgcccttag tgtttgtgct tctgcttgct tgtcactggc agcctctatg taggtttccc
      721 tatccactgc accagctgca cgtctagcag tctgtttacg gtctttgtcg cgctctaaag
      781 ccatgccttt actgataatc gtacgtagct ctctttgttc ggcatccgtg atttcaaaca
      841 gactgatcaa tgtgtcattg cggggcgtgt aaagcggtgc aaagtcttta ccgccaaagc
      901 tgaccttttc ccctgcctcg tactgtttcg cctttgaata cagcgtcatt aactctttgc
      961 tgttgtaacc ccagccagaa tcaacctctc gcgctaaagt cgcagcttcg tgatacatca
     1021 agctgctgtt agtagcaccc gataaaagca aaaagtttaa acgccagaat aagtggcgca
     1081 tacggtcgcc ctcgctcaca ccaccgcgca atgtggctaa agtgcgtagg tcttctagcc
     1141 tatgccatgc gagctggcgg ccactaaagc cccgtaggtt gctgctgact ttgtagccat
     1201 ccagcaattt aagctgctta cgcttctggc gttcatcacg ctcttgtttt tgtttttcaa
     1261 tatcccatct ggcgaccggc aaaagaattt ctgctaaata ctcaaagttg tagcgcacag
     1321 gctggccgtc tgctccgttt tcaacgtgga caacacggca gatttcattg cttttactgt
     1381 ttactgtttc gaccaggcga agtacccgac tagcatcttt agcgcctacg tctgcacctg
     1441 ctggcttcaa tcggtcaatt agataacgct ggcaagcgtt ccagcgaggc aatgcagcac
     1501 ggggcaacgt gccctctagc aaccacttag cctgtatg
//
